LOCUS       DANJFZ010000001        94475 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.001, whole genome shotgun sequence.
ACCESSION   DANJFZ010000001 DANJFZ010000000
VERSION     DANJFZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 94475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.001"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            347..748
                     /locus_tag="PWN45_000001"
     CDS             347..748
                     /locus_tag="PWN45_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDL0621805.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            708..800
                     /locus_tag="PWN45_000002"
     CDS             708..800
                     /locus_tag="PWN45_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621806.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1117..1446)
                     /gene="ecb"
                     /locus_tag="PWN45_000003"
     CDS             complement(1117..1446)
                     /gene="ecb"
                     /locus_tag="PWN45_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HDL0621807.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(1640..1807)
                     /locus_tag="PWN45_000004"
     CDS             complement(1640..1807)
                     /locus_tag="PWN45_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621808.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(1935..2438)
                     /locus_tag="PWN45_000005"
     CDS             complement(1935..2438)
                     /locus_tag="PWN45_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0621809.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(2431..3018)
                     /locus_tag="PWN45_000006"
     CDS             complement(2431..3018)
                     /locus_tag="PWN45_000006"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDL0621810.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(3030..3830)
                     /gene="racE"
                     /locus_tag="PWN45_000007"
     CDS             complement(3030..3830)
                     /gene="racE"
                     /locus_tag="PWN45_000007"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL0621811.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(4066..4881)
                     /gene="sdhB"
                     /locus_tag="PWN45_000008"
     CDS             complement(4066..4881)
                     /gene="sdhB"
                     /locus_tag="PWN45_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDL0621812.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(4881..6647)
                     /gene="sdhA"
                     /locus_tag="PWN45_000009"
     CDS             complement(4881..6647)
                     /gene="sdhA"
                     /locus_tag="PWN45_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDL0621813.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(6699..7313)
                     /locus_tag="PWN45_000010"
     CDS             complement(6699..7313)
                     /locus_tag="PWN45_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDL0621814.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(7637..9418)
                     /gene="uvrC"
                     /locus_tag="PWN45_000011"
     CDS             complement(7637..9418)
                     /gene="uvrC"
                     /locus_tag="PWN45_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL0621815.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            9584..9679
                     /locus_tag="PWN45_000012"
     CDS             9584..9679
                     /locus_tag="PWN45_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621816.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(9742..10056)
                     /gene="trxA"
                     /locus_tag="PWN45_000013"
     CDS             complement(9742..10056)
                     /gene="trxA"
                     /locus_tag="PWN45_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL0621817.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(10229..12577)
                     /locus_tag="PWN45_000014"
     CDS             complement(10229..12577)
                     /locus_tag="PWN45_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0621818.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(12587..14299)
                     /gene="polX"
                     /locus_tag="PWN45_000015"
     CDS             complement(12587..14299)
                     /gene="polX"
                     /locus_tag="PWN45_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDL0621819.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(14372..14893)
                     /locus_tag="PWN45_000016"
     CDS             complement(14372..14893)
                     /locus_tag="PWN45_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL0621820.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(14894..15160)
                     /gene="zapA"
                     /locus_tag="PWN45_000017"
     CDS             complement(14894..15160)
                     /gene="zapA"
                     /locus_tag="PWN45_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL0621821.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            15530..16468
                     /locus_tag="PWN45_000018"
     CDS             15530..16468
                     /locus_tag="PWN45_000018"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL0621822.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(16639..19041)
                     /locus_tag="PWN45_000019"
     CDS             complement(16639..19041)
                     /locus_tag="PWN45_000019"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL0621823.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(19041..20099)
                     /gene="pheS"
                     /locus_tag="PWN45_000020"
     CDS             complement(19041..20099)
                     /gene="pheS"
                     /locus_tag="PWN45_000020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL0621824.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(20480..21220)
                     /locus_tag="PWN45_000021"
     CDS             complement(20480..21220)
                     /locus_tag="PWN45_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL0621825.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(21460..21567)
                     /locus_tag="PWN45_000022"
     CDS             complement(21460..21567)
                     /locus_tag="PWN45_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621826.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(21604..21927)
                     /gene="isdG"
                     /locus_tag="PWN45_000023"
     CDS             complement(21604..21927)
                     /gene="isdG"
                     /locus_tag="PWN45_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDL0621827.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(21946..22680)
                     /gene="srtB"
                     /locus_tag="PWN45_000024"
     CDS             complement(21946..22680)
                     /gene="srtB"
                     /locus_tag="PWN45_000024"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDL0621828.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(22742..23707)
                     /gene="isdF"
                     /locus_tag="PWN45_000025"
     CDS             complement(22742..23707)
                     /gene="isdF"
                     /locus_tag="PWN45_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDL0621829.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(23720..24598)
                     /gene="isdE"
                     /locus_tag="PWN45_000026"
     CDS             complement(23720..24598)
                     /gene="isdE"
                     /locus_tag="PWN45_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDL0621830.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(24595..25671)
                     /gene="isdD"
                     /locus_tag="PWN45_000027"
     CDS             complement(24595..25671)
                     /gene="isdD"
                     /locus_tag="PWN45_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDL0621831.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(25671..26354)
                     /gene="isdC"
                     /locus_tag="PWN45_000028"
     CDS             complement(25671..26354)
                     /gene="isdC"
                     /locus_tag="PWN45_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDL0621832.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            26563..27615
                     /gene="isdA"
                     /locus_tag="PWN45_000029"
     CDS             26563..27615
                     /gene="isdA"
                     /locus_tag="PWN45_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDL0621833.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            27818..29755
                     /gene="isdB"
                     /locus_tag="PWN45_000030"
     CDS             27818..29755
                     /gene="isdB"
                     /locus_tag="PWN45_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDL0621834.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(29909..30082)
                     /gene="rpmF"
                     /locus_tag="PWN45_000031"
     CDS             complement(29909..30082)
                     /gene="rpmF"
                     /locus_tag="PWN45_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0621835.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(30162..30719)
                     /locus_tag="PWN45_000032"
     CDS             complement(30162..30719)
                     /locus_tag="PWN45_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL0621836.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            30846..31985
                     /locus_tag="PWN45_000033"
     CDS             30846..31985
                     /locus_tag="PWN45_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL0621837.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(32047..32529)
                     /gene="coaD"
                     /locus_tag="PWN45_000034"
     CDS             complement(32047..32529)
                     /gene="coaD"
                     /locus_tag="PWN45_000034"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL0621838.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(32531..33073)
                     /gene="rsmD"
                     /locus_tag="PWN45_000035"
     CDS             complement(32531..33073)
                     /gene="rsmD"
                     /locus_tag="PWN45_000035"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL0621839.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            33143..33532
                     /locus_tag="PWN45_000036"
     CDS             33143..33532
                     /locus_tag="PWN45_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621840.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(33535..33789)
                     /locus_tag="PWN45_000037"
     CDS             complement(33535..33789)
                     /locus_tag="PWN45_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL0621841.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            34028..34954
                     /locus_tag="PWN45_000038"
     CDS             34028..34954
                     /locus_tag="PWN45_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0621842.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(35017..35451)
                     /locus_tag="PWN45_000039"
     CDS             complement(35017..35451)
                     /locus_tag="PWN45_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0621843.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(35467..36504)
                     /locus_tag="PWN45_000040"
     CDS             complement(35467..36504)
                     /locus_tag="PWN45_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDL0621844.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(36831..37292)
                     /locus_tag="PWN45_000041"
     CDS             complement(36831..37292)
                     /locus_tag="PWN45_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDL0621845.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(37317..38228)
                     /locus_tag="PWN45_000042"
     CDS             complement(37317..38228)
                     /locus_tag="PWN45_000042"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDL0621846.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            38668..39579
                     /locus_tag="PWN45_000043"
     CDS             38668..39579
                     /locus_tag="PWN45_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDL0621847.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(39719..43171)
                     /locus_tag="PWN45_000044"
     CDS             complement(39719..43171)
                     /locus_tag="PWN45_000044"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL0621848.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(43725..44951)
                     /gene="ftsW"
                     /locus_tag="PWN45_000045"
     CDS             complement(43725..44951)
                     /gene="ftsW"
                     /locus_tag="PWN45_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDL0621849.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(45265..45540)
                     /locus_tag="PWN45_000046"
     CDS             complement(45265..45540)
                     /locus_tag="PWN45_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL0621850.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            45681..46163
                     /locus_tag="PWN45_000047"
     CDS             45681..46163
                     /locus_tag="PWN45_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621851.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            46165..46332
                     /locus_tag="PWN45_000048"
     CDS             46165..46332
                     /locus_tag="PWN45_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDL0621852.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(46472..48319)
                     /gene="typA"
                     /locus_tag="PWN45_000049"
     CDS             complement(46472..48319)
                     /gene="typA"
                     /locus_tag="PWN45_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL0621853.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            48421..48612
                     /locus_tag="PWN45_000050"
     CDS             48421..48612
                     /locus_tag="PWN45_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDL0621854.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(48766..49593)
                     /locus_tag="PWN45_000051"
     CDS             complement(48766..49593)
                     /locus_tag="PWN45_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDL0621855.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            49750..50364
                     /locus_tag="PWN45_000052"
     CDS             49750..50364
                     /locus_tag="PWN45_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDL0621856.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            50548..51900
                     /locus_tag="PWN45_000053"
     CDS             50548..51900
                     /locus_tag="PWN45_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL0621857.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(51982..52401)
                     /locus_tag="PWN45_000054"
     CDS             complement(51982..52401)
                     /locus_tag="PWN45_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0621858.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(52684..53703)
                     /locus_tag="PWN45_000055"
     CDS             complement(52684..53703)
                     /locus_tag="PWN45_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0621859.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(53777..54850)
                     /locus_tag="PWN45_000056"
     CDS             complement(53777..54850)
                     /locus_tag="PWN45_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0621860.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(54850..55659)
                     /locus_tag="PWN45_000057"
     CDS             complement(54850..55659)
                     /locus_tag="PWN45_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0621861.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(55665..56462)
                     /locus_tag="PWN45_000058"
     CDS             complement(55665..56462)
                     /locus_tag="PWN45_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0621862.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(56455..57549)
                     /locus_tag="PWN45_000059"
     CDS             complement(56455..57549)
                     /locus_tag="PWN45_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0621863.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(57562..58101)
                     /locus_tag="PWN45_000060"
     CDS             complement(57562..58101)
                     /locus_tag="PWN45_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0621864.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(58245..58520)
                     /locus_tag="PWN45_000061"
     CDS             complement(58245..58520)
                     /locus_tag="PWN45_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL0621865.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(58688..60094)
                     /gene="lpdA"
                     /locus_tag="PWN45_000062"
     CDS             complement(58688..60094)
                     /gene="lpdA"
                     /locus_tag="PWN45_000062"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL0621866.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(60098..61390)
                     /locus_tag="PWN45_000063"
     CDS             complement(60098..61390)
                     /locus_tag="PWN45_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDL0621867.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(61481..62458)
                     /locus_tag="PWN45_000064"
     CDS             complement(61481..62458)
                     /locus_tag="PWN45_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL0621868.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(62462..63574)
                     /gene="pdhA"
                     /locus_tag="PWN45_000065"
     CDS             complement(62462..63574)
                     /gene="pdhA"
                     /locus_tag="PWN45_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL0621869.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(63745..64371)
                     /locus_tag="PWN45_000066"
     CDS             complement(63745..64371)
                     /locus_tag="PWN45_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDL0621870.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            64736..65287
                     /locus_tag="PWN45_000067"
     CDS             64736..65287
                     /locus_tag="PWN45_000067"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0621871.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            65617..65739
                     /locus_tag="PWN45_000068"
     CDS             65617..65739
                     /locus_tag="PWN45_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621872.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            65768..65986
                     /locus_tag="PWN45_000069"
     CDS             65768..65986
                     /locus_tag="PWN45_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0621873.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            65986..67683
                     /locus_tag="PWN45_000070"
     CDS             65986..67683
                     /locus_tag="PWN45_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0621874.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(68032..68694)
                     /locus_tag="PWN45_000071"
     CDS             complement(68032..68694)
                     /locus_tag="PWN45_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL0621875.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(68827..69846)
                     /locus_tag="PWN45_000072"
     CDS             complement(68827..69846)
                     /locus_tag="PWN45_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL0621876.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            complement(69843..71204)
                     /locus_tag="PWN45_000073"
     CDS             complement(69843..71204)
                     /locus_tag="PWN45_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL0621877.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            71402..71635
                     /gene="nrdH"
                     /locus_tag="PWN45_000074"
     CDS             71402..71635
                     /gene="nrdH"
                     /locus_tag="PWN45_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL0621878.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            complement(71872..73590)
                     /gene="ptsP"
                     /locus_tag="PWN45_000075"
     CDS             complement(71872..73590)
                     /gene="ptsP"
                     /locus_tag="PWN45_000075"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL0621879.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(73593..73859)
                     /locus_tag="PWN45_000076"
     CDS             complement(73593..73859)
                     /locus_tag="PWN45_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL0621880.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(74013..74555)
                     /locus_tag="PWN45_000077"
     CDS             complement(74013..74555)
                     /locus_tag="PWN45_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDL0621881.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(74609..75781)
                     /locus_tag="PWN45_000078"
     CDS             complement(74609..75781)
                     /locus_tag="PWN45_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL0621882.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            complement(76206..77501)
                     /locus_tag="PWN45_000079"
     CDS             complement(76206..77501)
                     /locus_tag="PWN45_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621883.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGDFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            77653..77787
                     /gene="graF"
                     /locus_tag="PWN45_000080"
     CDS             77653..77787
                     /gene="graF"
                     /locus_tag="PWN45_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDL0621884.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     regulatory      78194..78294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            78371..78946
                     /locus_tag="PWN45_000081"
     CDS             78371..78946
                     /locus_tag="PWN45_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0621885.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     gene            78961..80361
                     /locus_tag="PWN45_000082"
     CDS             78961..80361
                     /locus_tag="PWN45_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0621886.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            80354..81160
                     /locus_tag="PWN45_000083"
     CDS             80354..81160
                     /locus_tag="PWN45_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0621887.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(81425..82672)
                     /locus_tag="PWN45_000084"
     CDS             complement(81425..82672)
                     /locus_tag="PWN45_000084"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL0621888.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(82694..84172)
                     /gene="purH"
                     /locus_tag="PWN45_000085"
     CDS             complement(82694..84172)
                     /gene="purH"
                     /locus_tag="PWN45_000085"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL0621889.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            complement(84187..84753)
                     /gene="purN"
                     /locus_tag="PWN45_000086"
     CDS             complement(84187..84753)
                     /gene="purN"
                     /locus_tag="PWN45_000086"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL0621890.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            complement(84756..85784)
                     /locus_tag="PWN45_000087"
     CDS             complement(84756..85784)
                     /locus_tag="PWN45_000087"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL0621891.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            complement(85777..87261)
                     /locus_tag="PWN45_000088"
     CDS             complement(85777..87261)
                     /locus_tag="PWN45_000088"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL0621892.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            complement(87240..89429)
                     /gene="purL"
                     /locus_tag="PWN45_000089"
     CDS             complement(87240..89429)
                     /gene="purL"
                     /locus_tag="PWN45_000089"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL0621893.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            complement(89422..90093)
                     /gene="purQ"
                     /locus_tag="PWN45_000090"
     CDS             complement(89422..90093)
                     /gene="purQ"
                     /locus_tag="PWN45_000090"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDL0621894.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            complement(90095..90358)
                     /gene="purS"
                     /locus_tag="PWN45_000091"
     CDS             complement(90095..90358)
                     /gene="purS"
                     /locus_tag="PWN45_000091"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL0621895.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            complement(90358..91062)
                     /locus_tag="PWN45_000092"
     CDS             complement(90358..91062)
                     /locus_tag="PWN45_000092"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL0621896.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            complement(91066..92190)
                     /gene="purK"
                     /locus_tag="PWN45_000093"
     CDS             complement(91066..92190)
                     /gene="purK"
                     /locus_tag="PWN45_000093"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL0621897.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(92177..92659)
                     /gene="purE"
                     /locus_tag="PWN45_000094"
     CDS             complement(92177..92659)
                     /gene="purE"
                     /locus_tag="PWN45_000094"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL0621898.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            92860..93720
                     /gene="folD"
                     /locus_tag="PWN45_000095"
     CDS             92860..93720
                     /gene="folD"
                     /locus_tag="PWN45_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL0621899.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
BASE COUNT        30712 a        17338 c        13386 g        33039 t
ORIGIN      
        1 tatatatcaa aacagctata tgtataacaa tttggagtgt ttgtctatcc aacttagcaa
       61 acaaaatgta gagaaatgta aatatcgttt tagcaataaa aattattcaa tcatagttga
      121 gtgacgttta ctactattat tatactatca acaaattcaa taatacctca aataaaaata
      181 ataccatcaa attcatcatt attctttttc gattgcatta aaccttatta atttgcactt
      241 aattttaatt aatgaagtgt taactggtaa atgtatgaaa ccgtttgtaa aatagcagtg
      301 tactcgcatt agtaagtaca aatttatttt acagggagaa cttattatga aaaaaaatat
      361 cacaaaaact attatcgcat ctacagtaat cgctgcaggt cttttaactc aaactaatga
      421 tgctaaagct ttctttagtt atgaatggaa aggtttagaa atcgcaaaaa atttagcaga
      481 tcaagctaaa aaggacgatg aacgtattga taagttaatg aaagagtctg ataaaaatct
      541 aactccttac aaagctgaaa ctgttaatga tctgtacctt attgttaaaa aattaagcca
      601 aggtgatgtg aagaaagcag ttgtcagaat taaagatggt ggtcctagag attactatac
      661 ttttgactta actcgtcctt tagaagagaa cagaaaaaat attaaagttg ttaaaaacgg
      721 tgaaatcgac tcgattactt ggtattaaaa aacatactga attaaatagt tgtacgccaa
      781 acgttagaaa acaatgctaa cgtattggcg tgctttttta ttttaagtaa cttccaattt
      841 atttagcatc tttacgactg tttaataaag cacgtatgat taacacggtt gccactatat
      901 ccgttacaat ttttatgatt gttaatacat tcgatttcct tttcacaata gccacctcgc
      961 ttgttcaaaa cataaacaac tattgcattc acttttaagt aattatttat attatttatc
     1021 ccaataagct caccattcaa ataaacacaa tattataaaa taattatacc tttggatata
     1081 gcaaaaagcc acactctata gcatggcttc tatcaattat tttaaagtat tatattttaa
     1141 aactagatcg atttgtcttt gtaatttttt cttttcataa ctgtgttgga aatgaattaa
     1201 attaacagct ctttgtgctt tacggtgtgt tgcaacagtt cttgtacgtt tgaaaaagtt
     1261 tacagctttt tgtgcatcca caactttttt atttacttgt tttttaaagt ttgtttgata
     1321 ctgatcatat tttttagcag cttcaacgtt tttagtttgt gcatgagatt caccggcaaa
     1381 cgttgatacc gttaaactaa tagcagctat gcttaaaatt gatttcccaa taaaattctt
     1441 tttcataatt aataacacct atcctcaaat atttattttg tatatattta taataataga
     1501 cagatacaaa aaactacatt aactatttat taactaaaca ttaatatttg tgtcactaca
     1561 tttctgaatt tcaagaattt tgctactcca tattttaacc atcaaattat tgatataatt
     1621 tatgacttta gaaatccttt taaatatgta tacgattcat gattttaact aaaatttcaa
     1681 atttcctgac ttcttggaat cttaatttta tactttttaa taatacactt ttaaccaaat
     1741 aatcaacatg attcaaccat atagggttat caacaatcac attctccaaa attacaattc
     1801 ttttcataac ataaatgtgt tattcattcc aattatctta taaaattgtc agaaatactt
     1861 ttaaacaaac attcatgaca cgacctccta gttaaatcag tctataaact aaaaaaagcc
     1921 atagctactt ctatttaaaa agtagtatga ctgattgttt cgtgatgtcg atttttgaaa
     1981 ttgatggtgc catgtaaagt ttgatcatca ataataactt cagcatatgt ttcttccatt
     2041 gaacttctag attgagatat acttccagga ttaataacat gaacaccatt aatatactca
     2101 tattttgcca catgtgtatg tccataaaat gcaaacaaac aacctaattc aagtcctttt
     2161 tcagctaata aatctcttgt tcgattgact tgatataaat gtccatgagt ataaaatgct
     2221 ttcacgtcat tatattttgc gaccgcttca ttttcaaatt ctgggtaaaa atcacaattg
     2281 ccttttactc tattaaataa gctaagttcc gtatcatcat acgcgaattc tgaatctcct
     2341 aaatgtaaat atacatctgc atctgggtgc atttcataaa tttgatataa aacgcctgat
     2401 tcagtatggt tatcactcac aataatccat ttagacattt ttatcacctt caagaaaagc
     2461 ttgaagtaaa ttaatcgcat ttcgtctatg actaatttgc cctttttgtt cttttgaaag
     2521 ttgagccatg gttttatcta atttcggtac ataaaatatc ggatcatatc cgaaaccatt
     2581 ttcgccatat tttccatctg caatttcacc tgaaacagta cctttaaata cttttgtttc
     2641 catatcaggg ccactcatac ttatgacaca aacaaattgc gcacgacgat cagttgtatt
     2701 accaagctta tttaataatt tttcaatatt tgcttcatcg cttttatttt caccagcata
     2761 acgtgcagag tatatacctg gctcaccatt taatgcaaaa acttctagtc cactgtcatc
     2821 agctatgacc gttttattca atgcttttgc agcagcttct gattttaata tagcattttc
     2881 ttcaaatgtt gatcctgttt cttccacatc aaaatctggt attagttctg aaatacctat
     2941 tacgtggtaa tctggaaata ttactttaaa gtcatttatt ttcccttgat tattcgatgc
     3001 aataacaatc tctttcatta aaaatcctcc tagtcattca ctgatatacg ttccacattg
     3061 acagataaat ttaaccactc tttgataatg ttagtaatgt gagtaggatc acctgttgca
     3121 aaaaatcgat gatctggatg ttcagtataa cttgcatgtt cattactaaa tgttagcaat
     3181 gcactaactt cacgagccgt ttctaatcca gacgaaatca ctgtcttttt accaccaaaa
     3241 taatcataga taggtttata gagcaatgga tagtgggtac atcctaaaat gacagtatca
     3301 gactcactat tacgccaacg tttcagtgtt tgatgaatga caatgcttgt aattgttgga
     3361 tcactatatc tcatttgttc tacaagtggc acaaaacctg gacaggcaac gccatgtacc
     3421 tctacatgtg gattgatacg tttaatatgc gtacgatatg cttcagattt aattgtgcct
     3481 tccgttccta gtactaatac attttgattt ctagtagtca ttattgctgt tctagcacct
     3541 ggttcaatta cgccaatcac tgggattgat aaggtctttt gtaaatattc taaagctaca
     3601 gcagttgctg tattacaagc aatcacgagc atttttatat caaattccat taatttacga
     3661 gcgatttcaa ctgtatattg ttttacttgt tctcctggtc ttggcccata tggacatcgt
     3721 ccaatatcac ctaagtaata aatcgtctca tttggcaact gacgcataat ttctttagct
     3781 actgtcaaac ctccgacacc agagtctatt acacctattg gtttattcat attcggtcat
     3841 ccttactttt aatttaattt ttagtttatc ataactaagc attggatttt agtattatgc
     3901 actgtgttta ccatttttgt cattataata tttattttaa atcagcccac tatcatattg
     3961 tcatgtaatc tgcttattaa aaaaatccct tccaagttat tgtgtatctc cattcaattt
     4021 aattttgaaa ggaacataac attttaactc aaaagggatt aatttttatt ctacttcatg
     4081 gtctgaacca aagaatgatt taaacatgtg gaatgttgtt tctctgttca ttgctgcaat
     4141 ggatgttgtt aatggaatac ctttagggca agcattaaca cagttttgtg aattaccaca
     4201 ctgctgtaag ccaccagtac ccattaatgc atttaaacgt tcatctttag tcatagatcc
     4261 tgttgggtgc aaattaaaca aacgaacttg cgagattgct tgtgcaccaa cgaatttatt
     4321 attttcagta acattaggac aaacctctaa acatacacca catgtcatac atttagataa
     4381 ttcataagct gtttgacgtt ttttctctgg catacgtggt cccggaccta aatcatacgt
     4441 tccatcaatt gggatccatg ctttcatacg ttttaagtta tcgaacattc tagaacgatc
     4501 aacttgtaag tcacggataa ctgggaaagt attcattggc tctaaacgaa taggttgttc
     4561 taattgatca acaatcgcag aacaagattg tcttgcacga ccattgataa ccatagaaca
     4621 tgctccacat acttcttcta agcagttcat atcccagaca acaggtgttg ttttttcacc
     4681 tttaatatta actgggttac gtctaatttc cattaaacaa gcaatgacgt ttaaattttc
     4741 acgatatgga atttcaaatg tttcttcata aggcttagaa tcacttgtat cttgtcgttt
     4801 aataattaat tttactgttt tttgtttcgg tttagattgt gtttcatgtt gtggagtgtt
     4861 tttcactgat tgttcagtca ttatttttta ccccctttag acttacttgt gtaatcacgt
     4921 ttacgaggtg gtattaaact cacatcgacg tcatcataag taaactgcgg tttttcaaat
     4981 gcgccttgga atgaggccat tgtcgttttt aaccactctt catcattacg ctctgggaat
     5041 tctggtttat aatgggcacc gcgtgattcg ttacggttat atgcaccaat cgtaataaca
     5101 cgtgcaagta ctaacatgtt ccatagttga cgggtaaaga ataccgcttg gttactccaa
     5161 gtttgagtat cttccatatc aatatcttca taacgtttca tcaattcaac aatcttttta
     5221 tctgtttcta acagtttttc attttcacga acaacagtta catttgctgt cataatttca
     5281 ccaagttcac ggtgtaattt atatgcattt tctgtaccgc gcatagctaa taatttatca
     5341 aaacgttctt gctcttcagc tttacgcttt tcaaaaatac tttcgtccat atcagtatat
     5401 gatcgatcaa tatttgaaat ataatcaatc gcgtttggac ctgctactgt accaccataa
     5461 atcgctgata acaatgaatt ggcacctaag cggttaccac catgttgaga gaagtcacat
     5521 tctccagctg caaataaccc tttaatattt gtcatttgat cataatctac atatagacca
     5581 cccattgaat agtgaacagc tgggaaaatc ttcattggta ctttgcgtgg gtcatcacca
     5641 gtgaattttt cataaatctc aatgatacca cctagtttta catctaactc atgtggatct
     5701 ttatgtgaca aatcaagata taccatgttt tcgccattta tacctaattt ttggttaata
     5761 catacatcga aaatttcacg cgttgcgata tcacgaggta ctaagttacc ataatcagga
     5821 tatttctctt ctaagaagta ccaaggctta ccatctttat atgtccaaat tcgtccacct
     5881 tcaccacgtg ctgattcact cattagtcgc agtttatcat caccagggat tgcagtagga
     5941 tgaatttgaa tgaactcacc attagcataa atagcgcctt gttggtaaac aatggaagcc
     6001 gctgatcctg tattaatcat tgagtttgtt gttttaccga aaataatacc agggccaccc
     6061 gttgccataa taactgcatc tgaaccaaat gtttcaatct cagcagttgt catattttgt
     6121 gcaacgatac ctcttgcact atcatcgtca cctttaacta tgccaaggaa ttcccatcct
     6181 tcatacttcg taactaatcc atctacttca tatgcacgaa cttgttcatc caatgcatat
     6241 aataattgtt gtccagttgt tgcccctgca tatgctgttc tgtgatgtaa tgtaccaccg
     6301 aaacgtctaa aatctaatag accttcattt gttctattga acattacgcc catacggtct
     6361 aataaatgaa taattttagg tgctgcctct gtcatcgctt taacaggtgg ttggtttgca
     6421 aggaaatcgc caccatacac tgtatcatca aagtgaatcc aaggagaatc gccttcccct
     6481 ttagtattga ccgcaccatt aatgccacct tgggcacaaa cagagtgcga acgctttact
     6541 ggtacaactg agaacaaatc tacatgtgca cctttttctg ccgctttaat tgttgacatt
     6601 aagcccgcta ggccacctcc gacaacaata agatgtttct ctgccataaa aatttcactc
     6661 ccctaaattt tcaatctata tttgttaaat gcgatgtatt acataaaggc aataattgca
     6721 gtaacaccaa tatacgaaat aactaaaaat acgattaatg aaacccatgt aaatactcgt
     6781 tgtgattttg gagattgaag tccaccccaa gtaactaaga atgaccataa gccatttgca
     6841 aagtggaaca caacagcaat aatacaaata atataaaata ttgcccatcc aggatgttgc
     6901 aatgtttcgt gcattaaatc gtaattcact tctttgccgt aaaatgcttt ttgtaaacgt
     6961 gtttgccata aatggatacc aataaagata aatgttaaga taccactcac tctttggaag
     7021 aagaacatcc agtttctaaa aatcgagtaa tgtccaacat tttcttttgc tgtaaatgca
     7081 atgtgtatac caaacaaacc gtgatataac aacggaatgt atataaataa aaattctaca
     7141 ataattagaa atggtaatga ttccataaag ttagatgcct tattaaacgc ttcagcacct
     7201 tgtgttgctt ggtgattcac taataaatga acgaccaaaa atgcacctat tgggataata
     7261 cctaataacg agtgaatacg tcttagataa aattcatttt ttgattgagc caaaaggagt
     7321 cccccctgtg aacgaatatt taatttattg agctatttat attaaacgta cgcttaaccc
     7381 cctaaagtga tatcgtttct agcgttgtca ttatcattaa gcgaaacatt ttaaagacaa
     7441 atacacactg cacgatcacc aaactgcatg tcgaacaatg taacatttgg attcgatatt
     7501 taaaattgct tgtgatgata aactttctca tttagaaaac gcttccacgt acattcaaaa
     7561 aaataacttt gttaaccata ttgtaacatt atttcatata ttttggggca tgagaatgat
     7621 tctcacgccc agtaatttat ttatgcaatt gttcatgtag gttctttgcg acgttttcag
     7681 gaatacctat atttttaaaa tcttcaagtg tagcttcctt cattttcttg attgaaccga
     7741 atgaacgcaa taataatgtt ttacgtttgt taccgatacc atctatatca tcaagtattg
     7801 atttcaagcc tgtcttttga cgtgtttgtc tatgaaatgt gattgcgaat ctgtgaacct
     7861 catcttggat acggtgcaac aaataaaatg cctggctatt tttcttcagt ggtacaattt
     7921 ctgcactagc gccatataat aattcagatg tttggtgttt atcatttttc tgcaaacctg
     7981 caacagggat atcaagacct aattcgtttt gtagcacatc aataaccccg ttcatatgtc
     8041 ctttaccacc atctactatt attaaatcag gtaatggtaa tccttcgttt aaaacgcgag
     8101 aatatcgtcg tcttactact tctctcattg atttgtaatc atctggacct ttaaccgttt
     8161 tgattttata ctttctataa tttttcttat ctggtttacc gtcgacaaat gtaaccattg
     8221 ctgacactgg atccacacct tgaatattag aattatcgaa tgcttcaatt ctaattggtg
     8281 tttgaattcc catttgtgtt ccaagttctt caatagcttt aatcgttctg gactcatcac
     8341 gtgatattaa ttcaaattta ttatttaagg atactttagc gttatgtgca gctaggtcaa
     8401 ccatatcttt tttgggacct cgcgcgggtt gaacgatttt agtgtccaca acagattgaa
     8461 tcatttcttt atccaaatta cgtggtacat gaacttcctt aggtaaaata tgttggttta
     8521 agctataaaa ttgtccaata aatgtataaa attcttcttc ttctgtttgc tgtaatggaa
     8581 tcatcgttgt atctcgcttt atcatattac cttgtcgtat aaagaaaact tggatacaca
     8641 tccatccttt atcaacacta taaccaaaga catcacgaat cgttttatct gatgacataa
     8701 ttttttgttt gtttgtcaga ttttgaatat gttgaattaa atctctatat tctttagccc
     8761 gttcaaaatc aagtgattca cttgcagtta acattcgctc ttctaaactt tttaaaattg
     8821 ttttgtcttc cccattcaga aaatcagtaa tttccttcgt catttgtgcg tatttactca
     8881 aatcaacgtc atatacacat ggtcctaaac attgtccaat atggtaataa agacataatt
     8941 tatctggcat cttatcacat ttgcgatatg gatatattct gtctaataac tttttagttt
     9001 cttgagcaga atatgcattc ggatacggtc cgaaatattt gccagtacct tgttttacag
     9061 ttctcgtcac tagtagtcta ggatatttct ccttcgtaat tttaataaat ggataacttt
     9121 tatcatcctt taataatata ttatatcttg gttgatattg tttaatcaga ttcaattcca
     9181 gtaaaagtga ttctgtttca cttgacgtga caataaactc aaagcgacgt atttcaccaa
     9241 ccagtctcgt tgttttagca tcatgagcac ccgtaaaata tgatcgcaat cgatttctta
     9301 gctttttagc tttgccaaca tatatcactt gatcattacg atctttcatt aaatagcatc
     9361 ctggttccat aggtacgaca tttaatttat ttttaattcg ttgcttatag tcttccaaaa
     9421 atatcccccc ttctaatttt aaaatgaatg atgctacaaa agtgcttcaa atttatcacc
     9481 actaattcta gttaataatc aacatcttcg agtatgactt ttcatgcaca tcatgtaata
     9541 ttgaacatgg ttttatcaaa ccaactgcta caaatctatc agcatgctag attatcattt
     9601 aacgttcata gatttattaa tcccaccatt ctcaagtcgt aaacaatatg atgacttatc
     9661 attcaacttt ttatattaaa aacacaaaca aaaactggag caattcatta aagaaatgcc
     9721 ccagtcgtca ttggttgtaa cttataaatg tttatctaaa acttcagcta agttttcttt
     9781 tggttggaaa ccaacaactt tatcaactgg ttgaccgtct ttaaagacga ttaatgttgg
     9841 aatactcatc acttcatatt tagctgcagt tgatggattt tcatcaacat ctaattttaa
     9901 aatgtcagct ttaccttcat agtcagctgc taattcttct aataccggag cgatcatttt
     9961 acatggacca caccatgttg cccaaaaatc tactagttgt acaccagatt ctacttttga
    10021 atcaaaatct gcatctgtta cttttacgat tgccataaat gccaatcctc cttaattatt
    10081 attatgatgt tcaaattata tcagataaca tgcttcatgt atttctattt gctcaatttt
    10141 tatgcttatt tcaaataaca tgtttgaaat gtattcttgt tgctttacat tattttaact
    10201 gcagctattt aatttatcaa attataattt attttagtgt tgcaacggta acgccaaatc
    10261 caccttcgct tggcatacca cctctaaagt cactaacact tttatgcttt ttcaaatgtt
    10321 gttgtacacc tttttgaagt gcacctgtac ctttaccatg aatgatataa acttgttcgt
    10381 aattacttaa aacggcttga tctaaatatt gatctagttc aattaaagca tcctcataac
    10441 gatagcctcg taagtcaagt tcagttttaa ttgtttgacg attttgacgt gtaaccattt
    10501 tcgttggctt aactttttct ttttgttttt tctctaaatc ttcaataggt aacttcattt
    10561 taataattcc catttgaaca attgcttctt catcattgac aatttctaaa acttcaccct
    10621 tttgaccgta agataatact tttacttcat caccagcaac aattttatcg tatttttgct
    10681 tttgtacatt ttgctttata gatttcgctt cataatgatc atctaatcgt ttcttcttat
    10741 caatcaattc atgttcttta acatctgcac ctttttgttc acgcaattgt cttaagtctt
    10801 taataatatc gtcagcttct tttgttgcag ccttaatctt ctgatttgct ttttctttcg
    10861 cttcctctat tagagacttt tcataatttt ggaattgttg atactgctta gataaatcat
    10921 catgcacttg ctccgcttct tttacaagac ggtccagttc taacctctgt gtctctacac
    10981 gtttgtaatt acgctctaat gattcaatca tttcatttat ttctttttca tcagtaccaa
    11041 tcatcgtctt agccttatta ataatattca aactaagacc taacttttta gaaatgtcaa
    11101 aagcatttga acgacccggc acacccatta ataacttata cgttggactc aaagtatcta
    11161 catcaaattc tacactcgca ttcataacgc cttctcgatt ataactatat gctttaagtt
    11221 caggatagtg cgtcgttgcc attactagag aaccaatttt tctaacatga tctaaaatgc
    11281 tcattgctaa tgcagcacct tcactcggat ctgtacctgc acctaattca tcaaataaaa
    11341 ctaaactatg tttgtctgca tgctttaaaa tttcaactat attcgtcata tgagatgaaa
    11401 aagttgataa tgattgttct attgattgtt catctccgat atcgcaatat acatttttaa
    11461 atacacttaa ctgactacca tcaagtgtgg gaatcaacaa tcctgattga gccataacaa
    11521 taattaaacc taatgttttt aatgttacag ttttaccacc tgtattcggt cctgtaataa
    11581 ttaccgtttc aatatcttcc ataaattcga tggtattagc tacaacagtc tcacgattta
    11641 ataatggatg gtatgcttta ggtaaatata cagtacggtc ctctttaaat atcggctttg
    11701 ttcctttaat acttctacta tatctcgctt ttgcgattaa aaaatctaac tgacccatga
    11761 cttgttctgc cacaagtagt gcatctttgt ccgcagccac ataaccagtt agttgcgtta
    11821 aaatgcgttc tttttcaatt gcttcgtcat gacgtaatcg actaatttga ttattcattt
    11881 caacaactga tgatggctca atatacaatg tttgtcctga agcagattga tcatgtacaa
    11941 tcccattaaa atcttgtcga tattcagctt tgacaggtat aacgtttctt tcattcctaa
    12001 ctgttacaat agcatctgat aattttttct gatttgcttg gcttttaaca atacggtcca
    12061 aattttgtct aatacgttga ttcgtgctag aaattttact tctaatccct tgcaattcat
    12121 aactcgcatt atcatataaa tcatacgtat cgcatgtttc atttatttgt tgaaaaagat
    12181 cagttaacac aggtaattga ttcatcttgt catctaatat tgggtattta acaccttcat
    12241 cttcttcaac caattgatta taaaatgtct tgaattgatt ttgtacttga attaatcttt
    12301 ttatcaagtt aagctctgat acatttaaaa cgccgccaat atcagcgcga tgaatgaatg
    12361 ctgatacttt agataagcca ctcaagcttg gtaaacgatg cttattatag atttgagcaa
    12421 tctcatccgt ttcttccatt tgaaaaacaa ccgtttcaaa attagtagct ggcatcattt
    12481 gattgacctt ttccaagcct aagtcactaa tagtttcatt ggcaacgagt gattttattt
    12541 tttcaaattc taagacgtct aatgtttttt gtctcataaa atccctctat ttcttaagtt
    12601 ttgtattatt ttcaataaag tctttaaaag cttcacgcga taatgcgtta atcactctat
    12661 cttttgttac aaatcctttt tgcgcagttg ctacgccata attcataaaa tctaaatgat
    12721 ttgtatgatg cgcatcagtg ttaatagtta atttcacatt tggatattta cgaacgatat
    12781 cagcgctcag atccagtcga tgtggattgg cattaatttc taatactgta ttcgtttctt
    12841 cagctaatgc cattaattgt tcaatattcg gtttataacc atctcttcta cctataatac
    12901 gccctgttgg atgcgctata tgtcgcacgt atggattgcg acatgcatta gctaatcgtt
    12961 ccataatttg ttcttctgat tggttaaagc tttgatgaat agctccaatt acataatcaa
    13021 gttgtgctaa aatttcatca tcataatcca gcgagccatc aggtaatata tccatttctg
    13081 tacctgaata aatatcaatt tcactatatt ctttatctaa agccttaatt tcttcgtttt
    13141 gtcttaaaag tctttctact tgtaagccat tagcaacacg taaactttgt gaatgatcag
    13201 taattaccat gaatttataa ccttttgcga tatttgcttc taccatgtct cgaatagaaa
    13261 acgcaccatc actatacgtt gtatgcatat gaatatcacc attaatatca tctattgtaa
    13321 tgatattact tagatcttta tcaaattcgc taccatcttc tcgcatagca ggtggtataa
    13381 aattcacatt aaaatgttca tatatcttgg cttcactatc atattgaatt aatgtaccat
    13441 cagcttgttc aattccatat tcacttactt tttcatcacg tgctttagca agttgtcgaa
    13501 ttcttatatt atgttctttt gacccagtaa aatgctgcaa tgtatgataa aaagcacttg
    13561 gttcaattaa tcgaaaatcg acaccaatcg tttcatcatc atacgctaat tctaatgaaa
    13621 cttttgtgtt ccccactgca acttctttta ctttattggg aatatttaat aattgctgct
    13681 gcactgcttt tgggttatcg gtacttatta tgaaatctaa atctttgctc atttctttaa
    13741 aacgacggaa gcttcctgca gatgaatatt gatcgatata atttaatgta tctatataat
    13801 caatgatttc ttgattaagt cttctcattt gatcaattgg atatctatct ttcttagcac
    13861 caagttgttt cacagcttct aatatgtttt gttccgtttt cttagcaaat ccgcttaatt
    13921 cactaacttt tccattttca caagcaactt gaagtgacgc tttatcaaca atattcaact
    13981 ctttatatag cttagcaatt ttcttgcttc caagtccttg aattttcaaa agtggaataa
    14041 gaccttccgg aacttcttcc tgtaattgct gtaaatactg agattcaccg gtctcacggt
    14101 aatcattgat tacttctgca acacctttac caatgccttt taactccgtt acatcagata
    14161 tttcatctaa tggtcgttca tctaattcaa gactttgagc tgcttttcga tacgctgata
    14221 ttttaaaagt attttcccct tttaattcca tataagtagc aatttgttct aatagtttga
    14281 taacatcttt ttttgtcata ataacactcc ataaaaagaa gaccaggacg tatcattaat
    14341 atataccttt gtcctgacct cttatgataa tttataaatt aatggtgaaa gccgataaat
    14401 atgggatgtg aaaaatcaat gaatgactca caatagatat tttaagctga tgttgtaatg
    14461 cttcgttagg atataatgcc aatagatata acgtaaagtg taagacaatt atcgtcataa
    14521 acacaccaac tatcattccc attgcacgac tgaaaatatg aatgttttga tacgctatta
    14581 ttttatcaaa agttacgata attagatata aaatgaactt acaaaacaat gtaatcatta
    14641 aaaaagctac aatcgcttca aatcgatttt gtagatgatt aaaatgaaac gcaaaagttg
    14701 tattaaatgc tgttgtttta ggatatggaa taaatacaat taatctttct acaatagatt
    14761 tgtaaaattg actggcaatc cacaatgata caatcgttgc actcaaatgt atcatagata
    14821 accaaaaacc tcgtctgaat ccaacgatga caaaatacac aaagaaaatg attatgataa
    14881 aatcaatgac catttattgc tcacgctgct gcaatttgtg aatttgttgt ttcaaacgtc
    14941 gattttcttc ttctagtagt actttttcat gcataatatt cacagcagtt agtattgctt
    15001 ttcttgaagt atctaaacct gctgctttat accctaattc ttttatttta tcatcaacta
    15061 aatgtgctac atatcgtatg tgctctgggt tatcttcccc aacaattgta aaaagctgat
    15121 cattaattga tacatttacc ttgtttttaa actgtgtcat ttataatttc tcctgatcct
    15181 ttttttaaaa tctaaattca cgttataaaa tatgactgga tagtttgtct gaatttgata
    15241 ctaatattgt tatattgcaa ttatgataaa acaacaacac aatctctata gatgacttaa
    15301 tgttcttttt ataatgaaat aatgtaaaga attttctatt caatacttta tcatgtttaa
    15361 attgtgtcac tataacattt tcataaacat tatacatgac cactatgtat tttgtaagta
    15421 tccgcaatta attctttaca acatacataa atgtttctga cgttattatc atttatgata
    15481 tgattatttt tgctaaagac aatgaaattt tatgaaagga tttacacaaa tggcgaatat
    15541 cgtttttaaa ttgtcggata aagacataac gacattaatg tcacgcattt cttttgatac
    15601 tgagaattta cctcaaggta tgaaagcacg tgcaaagtat caaaatacaa ctgtaaatat
    15661 ttaccaatca ggtaaagtca tgtttcaagg taatcatgca gaagctgtgt ctgaagaact
    15721 tctaccgcag cattctcaat tgaatactaa taaaactaaa aagaaaaata tggctaattc
    15781 ttttcttgaa caaactttaa tgtatgatca atttaactgt attggtagtg atgaggctgg
    15841 aagtggcgat tactttggtc cgttgactgt gtgtgctgct ttcgttacca aagagcatgt
    15901 ccctatttta aaaactttgg gtgtagatga ttcaaaaaaa ttaaccgata ccaaaattgt
    15961 agaacttgct gagcaattgg ttactttcat accacattca ttattaacac tacacaatga
    16021 aaaatataat attcagcaag caaaaggctg gactcaagta aaaatgaaag ctgcccttca
    16081 taatgaagcc atcaaaaatg tacttgaaaa aattgattcg tctcaattag actatatcgt
    16141 cattgatcag ttcgcaaaac gcgaagtgta tagccattac gcattatcag atataccact
    16201 tccaaagaaa acaaaatttg aaacaaaagg agaatcgaag tcattagcca ttgcagtggc
    16261 aagcataatt tctcgatacg catttgttac atatatggat caaatatcaa aaaatattaa
    16321 tatgacgatt cctaaaggtg ccggtgctaa agtcgatgta atcgccgcca aaattataaa
    16381 aaaatacggt ctttcacgtt tagataccat ttcaaaaaag cattttaaaa atcgtgaaaa
    16441 agcccaaaaa attttaaagc ctctttgatt tttaatcatt agaggctttt atgcagttgt
    16501 gcgaagcatc atctgtattc cgctgttcct ctttgacttt ttaaatgcat tggaaccaaa
    16561 ttggtgaacc caaaaataaa accccatgtt agggatatca atctacttca gatatcctta
    16621 tacatggggt ttaaatcatt atctaataac agcaccttgt tcaattaatg ctgcttcaat
    16681 ttccgcttgt acttttgaaa cgcgctcatc tgtcaatgtt tcttctgtgt ctaaataatt
    16741 taaacgtatt gcaattgatt ttttaccttt ttctaaatgt tcgccctgat atacatcaaa
    16801 gacaagtgta tcttttaata tattgccacc atgtgcatga atcgttgata ataaatcagc
    16861 tgctggaata ttttgatcta cttctaatgc aatgtcacga gacatgcctg ggaatctcgg
    16921 aattggctgg taattaatgt aacctaccga cacagccatt aatgcatcaa aattcaactc
    16981 aaaaacatac gtacgtttta aatcattatc agctgctaat attggatgta attcaccaat
    17041 aaaaccaaca actttattct ctaataagat ttcagcagta cgacctggat gtaatccatc
    17101 aatatcagca cggcgataac taaattcaag atttaacttt tcagatactc gatccacgac
    17161 accttttgct aaatagaaat caaccgtttc tttcttatct tgccattgat tgactacata
    17221 atctccagtt aaaataccac ttaaatattc aacttgatct ggtagttcac cttctccatt
    17281 agcaaagaag acattgccga tttcaaataa ttttacatct ttatttttgc gtgccacatt
    17341 atatgatgcc gcttcgatta aatgtggtaa taaactttga cgtaatgacg catgcgcttc
    17401 actcattggc atcaataaat caattgtttg acgctgttgc atcgaaaatg cagtagcatc
    17461 ttctttagaa actaacgaat acgtaatagc ttggtctaat ccagcacctt ctaacacttc
    17521 tttaaccatt ctagttttat attggcgatc agttagctga ccactagtaa ctttatcgaa
    17581 gacaggtaac gttgatggaa tatcgtcgta tccatatata cgtgcaactt cttcaattaa
    17641 atcttcttta attgtaatat ctttacgacg tgatggtact agcactgtaa taacatcatc
    17701 atttatttct gtatcaaacc ctagttgatt aaaaatagta acaatatcat tttgtgacaa
    17761 atcaaatcca atagtgcgat taattttatc agcagtgatg tcgattggtg taataaatgc
    17821 accaagttct cctgaagaca ctctatcttt tagcactttt ccgtttgcat aagtttgtaa
    17881 taaataacat gcacggtcga ctgcttcatc tacaaattca gtagctattc ctttttcaaa
    17941 acgactagat gattcactgc gtaaatttaa acgtcttgat gtatgacgaa ttgaaactgg
    18001 atcaaaaata gcaccttcaa tcactatatt tgatgtttgt tctttaactt ctgaaaaatc
    18061 gccacccata acaccagcta atgcaattgg agtttgtcca ttagtaatga caatatcgct
    18121 cgttaataat tcacgttctg tatcatctaa tgttgtcatt ttttcgcctt cattagcttg
    18181 acgaacaaca atttgttgtg aaccaatcgc atcttgatca aacatgtgca atggttgacc
    18241 gtattctaat aacacataat ttgaaatgtc aacaacatta ttaataggac gtatacccgc
    18301 ttttattaag cgtgcttgca tccaaattgg cgagggttca attgtcacgt cgtgaacaac
    18361 acgtgcacta taatatggta ctttatcttc attttcaatt gtcacagtca gttcatcatt
    18421 tgcagataaa tcaagctcat ttgatgttgt ctctggctta gtcatttttg tattatataa
    18481 tgctgcaact tcataagcag tacctatcat acttaaagca tctgcacgat tcggcgttaa
    18541 atcaaattcc attacttgat catctaaata taaagcttgt aaggcatctg ttcctggaac
    18601 ttgggcttca ctaaatacat aaatgcctga ttcaaaactt ttcggtatat agttacttga
    18661 aataccaatt tcttgtaacg aacaaatcat accttctgaa cgttcaccgc gtaatttggc
    18721 acgcttaatt ttaataccac caggcaatct gccacctact ttagcaacaa tgacatattg
    18781 tcctgcatca acgttcggtg caccacaaac gatttgtaca ggttcgtctt ctccgatatc
    18841 aacttggcaa acatttaatt tatcagcatc aggatgtttc tcttttgact taacgaagcc
    18901 gacaactaaa tttttgatat cttttgtgta gtcaattaaa tcatccactt caatacctgt
    18961 gcgcgtaata cgttctgcca aattacttac agaatcatcg attgttacat attctttcaa
    19021 ccattcattt gatatcaaca ttacatgtca cctctatctt ctaccgcttt aaattgatct
    19081 aaaaatctca catcattagt atagaaatga cgaatatctt ctataccata tttcaacatt
    19141 gcaatacggt ctggtcccat accaaatgca aatccagagt actctgaaga atcaaaacca
    19201 gccatttcta atacattagg atgtaccatt ccagcaccta aaatttcaat ccatcctgtg
    19261 tgtttacaca cattacaacc ttttccttta catttaaaac atgacacatc aacttctaca
    19321 gaaggttcag tgaatgggaa gtaacttgga cgtaaacgaa tttcacgatc agcaccaaat
    19381 aatttcttag ctaacaattc taaagtacct ttcaaatcac tcattttaac gtttttatca
    19441 acaactaatc cttcgatttg tgtaaattga tgactatgtg tcgcatcatc agagtcacga
    19501 cgatacactt taccagggca aataatttta actggacctt gaccatgacg tgattccatc
    19561 gtacgtgcct gcactggtga tgtatgcgta cgtaataaaa tttcatccgt aatatagaaa
    19621 ctatcttgca tatcacgtgc agggtgtgat ttaggtaaat tcagcatttc gaagttataa
    19681 tgatcttgtt caacttcata tccattcaca atttcataac ctaaacctaa gaataagtct
    19741 tcaatttctt ctattgtacg tgttaatgga tgctttgaac cgatttcaat atgacgacct
    19801 ggtaatgata catcaattgt ttcttcagcc aattgcttat ttaatttttc tttaactaac
    19861 atctgttgtc tttcatctaa ttcattttga attgtttgac gcaattcatt cactttttga
    19921 ccaaacgcag gtttatcttc attcggcaaa tccttcatca atttcattag tccgctaact
    19981 gacccttttt tacctaagta tttcacttta acttcttgca gtgcacgttc atcatttgct
    20041 tcattaatat ctacaagcgc ttgttgtttt aactctgaca ttgtttgttg ttcagacata
    20101 tgtcactgac ctccctattc gaactattca ttttctattt atcatatttg ttgtttaact
    20161 aaaaaaagac tttcatcctc ttaaaaaata agggacgaaa gtcatttccg tggtaccacc
    20221 caaatttatg cctaagcata cacttgttta atattgataa cggattgtct ttccgactta
    20281 aatctcttta agtaaccaaa aaggtgaaaa gactttaaat aattggaata acttgcagtc
    20341 acgattattc tccctaaaac aattatttat ataaatgtcc atgccttttt attggtttat
    20401 attcaattgt gtatgcattc acgtagaaat gttctgttaa acaattaatt ataattatat
    20461 cggaaaactt tcaacacggt caacctttca aatgataaag taaaatacta cctgcaatcg
    20521 ctacatttaa actttcagct ttaccataaa taggtatgat taaattttgt gtagtatgtg
    20581 ctaataattc aggattaaca ccttctccct cattacctaa tagtaatgca aaagaatcac
    20641 ttgaagtaac ttctttgtat gccactgcgt tttcaagtgc tgtaccataa acaggaccat
    20701 taaattgagt aataaacgta tcgagatctt gtgtcataac tggcaaatgg aaaacactac
    20761 cttgactcgc tcgcaacact ttatcttgat aaggatctgt cgtacccttc tccattatta
    20821 cagcatccat tccagcagca tccgcagttc taattaatgt gcctaaattt ccaggatctt
    20881 gaacacgatc gatgagcaat acttgttgtg ctttagaaat atcataatgc ggcttctcaa
    20941 tgattgcgaa aaacccttgt ggtgtcactg tacctgataa agattcagca actttcatgt
    21001 ttatttcaaa aacttcttgc gcgtatgcga taatttgctg atctaatctt gccggttcaa
    21061 ttgcaaataa ttgtgtaatt acaattccac tttgataagc ttcttcaatt aaatgcacac
    21121 cttcaattaa agctaatcca gttttatccc tctcacgttt cttttttagc ttgttcgctt
    21181 gtttaattct attattttgt gcagaagtaa tttgttccat tgatagctcc tcgctttatt
    21241 tttaaaaata aaaatattaa tcattaataa gatgaaaaca tttgattgta tagttaatat
    21301 taattaatcg cttttatcac tcataatatt tcaaattgta taaatttctt ttatcgatac
    21361 tactactata aatcatacgc cccaaaatat cattattaat tcttttcttc ttcaaaataa
    21421 atcaaaatga tataattgat gattattttc aaagcacatt caaatcaaac tatgttttag
    21481 caatttgttg ttagcatgtt tgtgttcatt aaaaaaacga ccatcatcgg tatcatgtat
    21541 ggtcgttaca aaagctaaca ataccaattg tcataacaag tactgcaacc tctttaaatt
    21601 caattatttc atgtaactat agcctatatc atatgtaatt actttgttat ttataatcgg
    21661 gctactttca tcttcatttt tacttctaac atgtttatgc gctgctttaa agacatcaga
    21721 ttttaaccaa tccgtaaaag cttgctttga tttccaaact gttaaaattt tcacttcatc
    21781 aaaatcttct tgttctaaag tttgtgtaac aaacatgcca tcaaagcctt ctaatgtttc
    21841 aatcccatgt ctcgtgtaaa atcgttctat aatatctttt gctgttcctt ttgttaacgt
    21901 cagcctattt tctgccataa atttcataat tatcctcttt tctgtttaac ttaccttaat
    21961 tatttttgcg acaacaacaa ttcttttcgt cgtttcacta tatgcatctt cgcacgttga
    22021 taaagtcatt attctatctt ttaccgttac attaacatct gaattaatta cagatttacg
    22081 ttttgtctca tctaaaaatt gttgataatc ttgatcattt tcaaaatctg tacgtatgta
    22141 attatcttta gtagtagttt tatatgcact aaatacttgc aattgatatt taccatattt
    22201 attgtcaaat tcaattatct tgtgtttttc ataaaacgat tgctttaaat aatcttctaa
    22261 cacatcaaac atcgtattat caccgacatg gtgcccgtat aaaatagtat tatgatttaa
    22321 attcttcaat tcatttctaa aatccataaa aatactacct ttacgtcgat gttctcgctc
    22381 aaaatctaaa tttaaataat cgtgatttgt cttaccttgt agtactggat aatttaatga
    22441 tgttcctgat aattttatcc atccaacaat gtctttattt attttttcaa gtgattcaaa
    22501 ttgtggtctc acatgttctt gatgtttgct catcagcatt tgaaattttt gttgtaattt
    22561 ctcataattt gcgcgttctt gcttgtcttc aatatatgtt tgaacaattt tgtaaccaaa
    22621 aatgataata attacaacca ataaaatttg tacaatagtt aaaaatcgct tcattctcat
    22681 aaaaatcctc ttttattaac gacgtttctt cagtcatcac taaaccagtt gttgtaccgt
    22741 tttagattcg atttcgttga ctttgacaaa ttaagtaaat tagcattgga ccaccgacaa
    22801 tcattaaaat agcattggct ggaatttcta aaggaggctg tatcactcgt cctaataaat
    22861 cagccactaa caatagccat gcaccaataa ctgtagaaaa cggaataagt actctgtaat
    22921 tgcccccaac tagctttcta accacatgtg gcacaataat acctaaaaag gctagttgtc
    22981 caacaatcgc aacagttgca cttgctaaaa atactgctaa taaacctgtt aaccatctgt
    23041 aacgatcaat attaaaaccg atacttcgcg cttgtatgtc gtctaaattt agtaaattca
    23101 atttagggga caatagtaat gttaatatta atcccaataa tgctgatact gctaatatgt
    23161 atacgtcgct ccatattttc attgttaagc cttgaggaat tttcattaaa gggttttgag
    23221 ttaaaatttc taaaacacca tttaataata cgaataacgc aacacctact aatatcatac
    23281 ttacagcatt gaatctaaat ttagaatgca acaatataat tattaaaaat ggtattaaac
    23341 ctccaataaa acttaataat ggtaagtaaa agtacaattg tggaataaac aacatacaaa
    23401 gtgctctcat tataagtgca cctgaggaaa cgccaatgat attcgcctct gccaaaggat
    23461 tttgtagtgc tgcttgtaat aatgctccag aaactgctaa cattgcgcca accatcaatg
    23521 caattaatat acgtggcaat cgcaaatcaa tgattgaatc cactgcttca ttgctaccag
    23581 ttgtaaattt tgtaaatagg tcattaaatg acaatttaat tgtaccggtt acaaacgaaa
    23641 tataagcagt tgcgattaaa atgactaaca aacataaaaa tagtagtttc tttttatttt
    23701 ttatcatcat atcaactcac tattttttat ccttataaaa taaatcatat aattgagtca
    23761 ttgccttatc agcatcaaca tttgctgtaa taccgaatgg cacttcctct aagtcataaa
    23821 catgattatt tttcacagct ttaaaatgtt tccaaatatc attttgttta aattcttttt
    23881 gaaacatttt cttaacttct tcaggcattc cgtgtggtaa tcgtaaaata atatctggat
    23941 tgatattcaa caaattttca gtattagacg aaatatattg acgatctttc actttaataa
    24001 cattttctcc acctgctatt ttaactaaat caccaatata tgatttatca gttgctacta
    24061 aatagctacc cggtacaccc attaatatta atactttggg atgtttcttt tgtttagctg
    24121 ctttattttc aattttttgc tttacagaat ttaaatggtc atttaattct tttgcttgtg
    24181 ctttacgatt aaattgatca cctaattgtg taatcgactt ttgcatccct tttaaactat
    24241 caaaatcata aaagtagcct ttcatattta attgtttgta aaatggttgc atttcatctt
    24301 taatcgttga cacactcaaa acatgtgttg gttttaactt tttaacagct tcaacattcg
    24361 gctccattgg ttgaccaatt tccggtacat ctttataacg attaggcaat gtcttatatg
    24421 acgtgggttt gccgacaatt ggtaaatcca atttgtccaa tgtcattgtc aatgcaacag
    24481 ttgttggtac gattctgaat tcgccggatt tagttgattc ttgagaactg gaagattgac
    24541 agctggttaa gataacgacg cttatcacta aaatggttaa atactttatg attctcaaac
    24601 ttggttacct ctctttctgc tgtataaatg tgccgaaata agtaaaatag ttgctaacac
    24661 aatgccagct gcaaataaca tatattgctt tttatgattt ggttcatcaa gttctttaat
    24721 atcactagat aattgactac tattcttgct atcatcatct ctattaaaag ctggtaacgc
    24781 tttttctttt tcaactactt ttttcttttc cttttttgat ttttcatctt tatgtttcgg
    24841 ttgctctttg tgattatttt caaccttttt tattgttgga gggctatttt caacttttaa
    24901 tgtacctgat gctttaggtc gctcattttc aatcttttta ttactgtctt gttgcacttt
    24961 atctttttgt acaggaatat catttaatcc tttttcagta aaaaggctag tacgtctttc
    25021 cttagtatct gatttttgac tatctttttt gtcatcaata tcttttttaa gctgatcttg
    25081 attattttct tgattttgat gttttgaagg cacttctttt gcatcatcat gttgtacttt
    25141 tttctcttta aaatgaatag aatacaccat ttctttgtta tctagatttt tcttttcagc
    25201 caacgtttta acatgcatat tgattttatc catatgctgt tcaattaaaa ttcttactat
    25261 tgtgtcatcc ttttcatctt ttataacaaa aggaaattct tcatttttgt cattttgaaa
    25321 aacaagttgt tctttatcta tttgaccttt tagctttact tgcaagtaaa ttttaccagt
    25381 catatctgaa atcaattgcg ccttttcgtc aactagttgt tgttcctcag ttacattttt
    25441 attttcttga ttaaaaatta gtaactctaa cgctttattc tcttttatct tttcatcaaa
    25501 aattgatgta gttaattctg ttttatcttt tgcttcaggt tgattaaatt tggctgatgg
    25561 atattttatc tcatcagcca acacgctacc aatcattgtt gtatatgtaa gtatacctaa
    25621 gctaataata gctataatag attttgtagc aatttgttta acatttctca ttattccaca
    25681 ttgcctttag atttttttct aacaaacaat gtgattgcga ttaataatgc taaggatgca
    25741 actggtattg catatatata tgcaggtgta ccagcatttg tttgtggatt ttctactttg
    25801 tctttattcg aactattaga ttcactttga ccagtagtcg ttccatcaga tcctttgtca
    25861 ctacctgaag cagaattttt atcatcttta cctggtgcat tagcacctgc tacatcagtt
    25921 ggtccattaa atttatatgt aatgttgtaa tgatggtcat atttgaatgg ctttccattt
    25981 actttttcat cgatataaac gtcaattttt ccatctattt taccgttcaa cttacttact
    26041 tcaaattcag aagtgcgttc atctttggca gtgtttttac taataatatt ttctttatgt
    26101 ccttcgatac tcattccagt aatccaatga ctgtggttga cagttatttg aacatacaat
    26161 ttaccatttt tcttaatgta ctttgccggt ttattaaaat agtcattagc aattgacgtg
    26221 tcattggtat tgtatttgta aacctcataa ttcaaagtac cgctatctgc ggcatttgca
    26281 gaattactga atgtcgcgat gatgataatt aacgctaaaa tcgttgtatt aaaaactttt
    26341 aaaatatttt tcaaaacata atcctccttt ttatgattgc ttttaagtct ttagtaaaat
    26401 cataaataat aatgattatc attgtcaata tttattttat aatcaattta ttattgttat
    26461 acgaaaatag atgtgctagt ataattgata accattatca attgcaatgg ttaatcatct
    26521 catataacaa cacataattt gtatccttag gaggaaaaca acatgacaaa acattattta
    26581 aacagtaagt atcaatcaga acaacgttca tcagctatga aaaagattac aatgggtaca
    26641 gcatctatca ttttaggttc ccttgtatac ataggcgcag acagccaaca agtcaatgcg
    26701 gcaacagaag ctacgaacgc aactaataat caaagcacac aagtttctca agcaacatca
    26761 caaccaatta atttccaagt gcaaaaagat ggctcttcag agaagtcaca catggatgac
    26821 tatatgcaac accctggtaa agtaattaaa caaaataata aatattattt ccaaaccgtg
    26881 ttaaacaatg catcattctg gaaagaatac aaattttaca atgcaaacaa tcaagaatta
    26941 gcaacaactg ttgttaacga taataaaaaa gcggatacta gaacaatcaa tgttgcagtt
    27001 gaacctggat ataagagctt aactactaaa gtacatattg tcgtgccaca aattaattac
    27061 aatcatagat atactacgca tttggaattt gaaaaagcaa ttcctacatt agctgacgca
    27121 gcaaaaccaa acaatgttaa accggttcaa ccaaaaccag ctcaacctaa aacacctact
    27181 gagcaaacta aaccagttca acctaaagtt gaaaaagtta aacctactgt aactacaaca
    27241 agcaaagttg aagacaatca ctctactaaa gttgtaagta ctgacacaac aaaagatcaa
    27301 actaaaacac aaactgctca tacagttaaa acagcacaaa ctgctcaaga acaaaataaa
    27361 gttcaaacac ctgttaaaga tgttgcaaca gcgaaatctg aaagcaacaa tcaagctgta
    27421 agtgataata aatcacaaca aactaacaaa gttacaaaac ataacgaaac gcctaaacaa
    27481 gcatctaaag ctaaagaatt accaaaaact ggtttaactt cagttgataa ctttattagc
    27541 acagttgcct tcgcaacact tgccctttta ggttcattat ctttattact tttcaaaaga
    27601 aaagaatcta aataaatcat cgtcacactc ataacttaat atatttttta ttttaaattt
    27661 tatttaacct atgtcataga tatttcataa tctataacat aggttatttt tttataaaat
    27721 aacgttgcaa ttaactaaca tttcaatgta caatacaagt aatcaattga taatgattat
    27781 cagttgataa tatacaatta ggagttgttt ctacaacatg aacaaacagc aaaaagaatt
    27841 taaatcattt tattcaatta gaaagtcatc actaggcgtt gcatctgtag caattagtac
    27901 acttttatta ttaatgtcaa atggcgaagc acaagcagca gctgaagaaa caggtggtac
    27961 aaatacagaa gcacaaccaa aaactgaagc agttgcaagt ccaacaacaa catctgaaaa
    28021 agctccagaa actaaaccag tagctaatgc tgtctcagta tctaataaag aagttgaggc
    28081 ccctacttct gaaacaaaag aagctaaaga agttaaagaa gttaaagccc ctaaggaaac
    28141 aaaagaagtt aaaccagcag caaaagccac taacaataca tatcctattt tgaatcagga
    28201 acttagagaa gcgattaaaa accctgcaat aaaagacaaa gatcatagcg caccaaactc
    28261 tcgtccaatt gattttgaaa tgaaaaagaa agatggaact caacagtttt atcattatgc
    28321 aagttctgtt aaacctgcta gagttatttt cactgattca aaaccagaaa ttgaattagg
    28381 attacaatca ggtcaatttt ggagaaaatt tgaagtttat gaaggtgaca aaaagttgcc
    28441 aattaaatta gtatcatacg atactgttaa agattatgct tacattcgct tctctgtatc
    28501 aaacggaaca aaagctgtta aaattgttag ttcaacacac ttcaataaca aagaagaaaa
    28561 atacgattac acattaatgg aattcgcaca accaatttat aacagtgcag ataaattcaa
    28621 aactgaagaa gattataaag ctgaaaaatt attagcgcca tataaaaaag cgaaaacact
    28681 agaaagacaa gtttatgaat taaataaaat tcaagataaa cttcctgaaa aattaaaggc
    28741 tgagtacaag aagaaattag aggatacaaa gaaagcttta gatgagcaag tgaaatcagc
    28801 tattactgaa ttccaaaatg tacaaccaac aaatgaaaaa atgactgatt tacaagatac
    28861 aaaatatgtt gtttatgaaa gtgttgagaa taacgaatct atgatggata cttttgttaa
    28921 acaccctatt aaaacaggta tgcttaacgg caaaaaatat atggtcatgg aaactactaa
    28981 tgacgattac tggaaagatt tcatggttga aggtcaacgt gttagaacta taagcaaaga
    29041 tgctaaaaat aatactagaa caattatttt cccatatgtt gaaggtaaaa ctctatatga
    29101 tgctatcgtt aaagttcacg taaaaacgat tgattatgat ggacaatacc atgtcagaat
    29161 cgttgataaa gaagcattta caaaagccaa taccgataaa tctaacaaaa aagaacaaca
    29221 agataactca gctaagaagg aagctactcc agctacgcct agcaaaccaa caccatcacc
    29281 tgttgaaaaa gaatcacaaa aacaagacag ccaaaaagat gacaataaac aattaccaag
    29341 tgttgaaaaa gaaaatgacg catctagtga gtcaggtaaa gacaaaacgc ctgctacaaa
    29401 accaactaaa ggtgaagtag aatcaagtag tacaactcca actaaggtag tatctacgac
    29461 tcaaaatgtt gcaaaaccaa caactgcttc atcaaaaaca acaaaagatg ttgttcaaac
    29521 ttcagcaggt tctagcgaag caaaagatag tgctccatta caaaaagcaa acattaaaaa
    29581 cacaaatgat ggacacactc aaagccaaaa caataaaaat acacaagaaa ataaagcaaa
    29641 atcattacca caaactggtg aagaatcaaa taaagatatg acattaccat taatggcatt
    29701 attagcttta agtagcatcg ttgcattcgt attacctaga aaacgtaaaa actaataaat
    29761 cgtctttata tttaattatt aaattaacaa attttaattg gcggatgagg tatccagtta
    29821 cctcgttcgc caattatttt tcgcaatata aaaagtccca cttaaaacaa tcattttaag
    29881 cgggactttt tatattgagt aactaaaatt atttagctgc tacttcttcg ccattgtaag
    29941 aaccacagtt tttacataca cggtgtgata atttgtattc gccacagttt gggcattcag
    30001 tcatacctgg tactgaaatt ttgaaatgcg tacgacgttt gttttttcta gttttagaag
    30061 ttcttctttt tggtactgcc atgatatatc ctccttagat tataaacgaa aaatactaaa
    30121 tgttagttta attaacaaca ttatatcatt aattaaacta cttattgctc tttatcatat
    30181 aattgttgta atttttgaag ccttggatca acttgtcgtg attctgaatc atcttgttct
    30241 tgctgtttag caagctcatc taattgatcc tcatcgatta cttcccaacc attacctact
    30301 gtcaacattt ggtcactttg ctctgaataa gctctcattg gtttctcaat aataactata
    30361 tcctcgacaa tatcctgaag attaaccata ccatctttaa taatgtgata gtgttcatct
    30421 acatcatctt gatcatcgtt atactgattg tacccttcta aatcaaatac ttctgtagta
    30481 gttacatcta gtgggacttt tactggtaca agagtacgtg cacaaggcat tgtatacgtt
    30541 ccagtaatgt gaatatccgc aacgacttct gttgacttaa tggttaactg accttggatt
    30601 gtaattggag ataaatcaat taaatctaat gattctttta aattgtcaaa actcaccgtt
    30661 tgatcaaatt caaatggctt accttgatat ttccttaatt gcgtaattga ccatttcata
    30721 tggcttcacc tctaacaagc acaaaattta ttttaacttt ctaagaacaa gttgtcaaga
    30781 ttttttctta acatatcatg ttctgttaca atattaataa tgaataccga aaggacgaat
    30841 tgcaaatgaa aagcgttggc ttaatcacag aatataatcc ctttcataat gggcatcaat
    30901 atcatattaa tcaatctaaa aaacttacaa atgctgacgt tactattgca ataatgagtg
    30961 gtaactttgt catgcgtggc gaaccagcaa tctataataa gtttactcgt gcaaaaatgg
    31021 cattatcaac agctgattta gttatcgaac taccagcaac tgccagttta tcatctggcg
    31081 atcattttgc cgaactagca gttaaagtcg cagattatat gagtgtcgat acaattgcat
    31141 ttggtagtga aaataatgat atcaaaacat taaagcaatt agcacacagc attaatgaaa
    31201 ttgaacaatc tgaatccttt tcacaaaaag taaaagaagg taaaagctac cctcgtatca
    31261 ttagcgaatt attagagcat catgaagcac tagcaagtcc taacaacata cttggtatta
    31321 gttacctgaa agcaattgct aaaaatgcta aaaacatcaa tgcaatttct atcaaacgag
    31381 aaaatgctca acatcatgat tcattaattc aacaccatca gtttgcaagt ggtacatcta
    31441 ttagaacatc aatcattagt caagatgatc attggcatca tgtggtacct aaagatattc
    31501 aacacttgta cgtaacacct catataacat taaatcaaat atttccatat ttgaaatatc
    31561 aaatcatagc tatgactaca gattcattaa aaaatattta tactgttact gaaggttttg
    31621 aaaatcgttt gaaatctaac atttatgaag caactgattt tcatcatttt gtaaaattac
    31681 taaaaacaaa gcgttataca tatacgcata tccaaagact gttaatgaat gtattattaa
    31741 acattaaacc tacagatgtt acgagtaaca ttcacgcagt taaagtatta gcgatgaacg
    31801 accgtggcag acaatattta aagcatttaa aaacagcgtt cccagaaaga caatatataa
    31861 ccaatattaa caaaagcaat gcacattact tcacaaatga aatcaaagca acacacatat
    31921 acaatgcaat aagcgggcaa caacaaacag atttcaacac gcctgtcata cagcaatatc
    31981 gttaacaaat gaaaacagta ttttaggatt gtaaaccatg ataaacctaa aatactgtta
    32041 tttttattac ttaaatttct tcttcaatgc cttttcaaca taaggtggaa cgaattcaga
    32101 aatatctgct cgataagctg caacttcttt aacaatactt gaacttataa atgaataatt
    32161 agtactagac atcatatata acgtttcaat ttcattgttc aactttttat tcattgaagt
    32221 taagcgtaat tcatattcaa aatcactgac tgctcttaaa ccacgtatga ttgttttagc
    32281 tcctacttgt tcacaataat cgactagtaa accactaaat tgatgaacct tgacattagg
    32341 taaatgttta acagattgtt caattaaatc catacgctct tctaaactaa acgtaccttc
    32401 ttttttacta tttttaagaa cacagacatg aatttcatca aatctatctg tacttctctc
    32461 aataatgtct aaatgaccat aagtaatggg gtcaaaacta cccggaatga ccgctattgt
    32521 atgttccatg ctattctccc ttttctaata acaatgtgtc tgtcaaccca taatggtaac
    32581 gtttaatcat attaaacggt tgataatcta tttcttcatg attgctaaat tcacaaacga
    32641 tgataccatt ttctttcaat aaattaaact ctgaaattag ttttaaagct ttatcaatga
    32701 gacctttatt ataaggtgga tctaagaaaa tgacatcaaa ttgaatatca cgttttgaca
    32761 atgcttttaa agctctatct gcattatttt tataaacttc agattgtgcc tctaaatcca
    32821 aattcgcaag atttgattta ataactttta cagctttaaa attttgatca acaaagatta
    32881 ccttatccat acctcgagag agtgcttcta ttccaagcgc cccgcttcct gcaaataaat
    32941 ctaaacctat acctgacaca tcatataaac tattaaagat accttcttta actttatcca
    33001 tagttggtct cgtattacgg ccttccatac tttctaaagc tttactttta tgtttacctg
    33061 caatgacgcg catgttgttc acacttccaa ttcatttagt tatttaatat aatttattga
    33121 gaaaaaggag aatgataaac caatgaaaca aacatttatt acacttggtg aaggtctaac
    33181 agatttgttc gaattcatga cgatgattga atataaccat caacgtattg ataaaattat
    33241 ctattttcat tcaccacaag ctgaaaataa aaagtcatct gtagcaatca ttatgaaccc
    33301 tacaactggc aatcatttcc aagcatttta tatcatgata aacgctatta aatatccata
    33361 tccagattca aataaaaagt ttcaaatgat aaatgattgt gctgaaaaat tcgacatacc
    33421 aattttaggt atcgatgtac agccccctca agcatttcat gatttatcgt tatattataa
    33481 ttatttaatt agtgtgttaa ggctccaaaa atggatacca gaacttcaat aatattaatt
    33541 atatatttcg tgtttctctt tttcgtaagt tttctttaag tatttatatg gtgagccatc
    33601 aatgtgtcta acgtatttaa gttgcattag tttatgtaca atttgatcaa catcttgctc
    33661 attcacatac ataattacaa atttacgatc tctatttgaa tgaacgatat gtccatattt
    33721 tcggatttgt cgttcatgtt tcatatgttt taaataaact acaatactag ttcttgggat
    33781 taaattcatt taaacctcac tccatttatt ttcagcatta atcatatcat gtctttatta
    33841 attttaaaat attaagctac cttaattatg tcttatgtaa ttgctcgata aatgtattta
    33901 gattatatta aacatcatta tattaattca attctcaaga gcatcactat atgattaacc
    33961 acacatttcg ctgtagtcag aactatgaat ttgttaagct ataaaagtaa acgaggagga
    34021 attttttatg aaaaaagtaa acagaaacat tttaaatgcg gtactagtcg gtgctggatt
    34081 tttaagtagt ctattaatga tcaataaaaa taaagtgatt actaaaaagc aaaccatccc
    34141 tgcttttttc aaaggcaatg caccatatat atttgcgcat cgcggtggta tggctttaag
    34201 accagagcaa acgcaacttg catttgacta tgctaaacaa ttaggcgttg atggctttga
    34261 aacagatgtg agactcacaa aagatcaaca gcttatcgtt tttcatgacg caactgttga
    34321 tagaacgacg aacggctctg gaaaagttag tgcccataca ttagcagaat taaagaaatt
    34381 agacgcagct tatcacttca aagatatcaa tggactaaca ccatatcgtg gtcatgcaca
    34441 cacagctata ctaaccttcg atgagttgct gaagcaatat cctgatatgt atataaacgt
    34501 agacttaaaa gatgctcctg aatcctatga aggctcaatc gcaccacaaa ttatgtttga
    34561 tacaattgca gaaaatcaag catttgaccg cgttcttgtg acaagttttt ataaagaaca
    34621 aatcgttcgt tttaataaaa ttgcacaagg atccgtcgca attggtgcta gccaacagga
    34681 agttacagaa gcatttttaa aatatcattt attaggtggt aggtactacc aaccattagc
    34741 acaaacgttc caaatgccta ctcattttaa aggcattgat ttgacttcat ctcgttttat
    34801 caaatggtta aatgatatga atatcattcc aggctattat ggcgtaaata gtataaatct
    34861 gatgaatgat ttataccaaa aaggtgcaca cacgattgtg acagatcgac cagatttagc
    34921 acaacaattc aaacaaacaa tcccgaataa ataaaaacat cgagtcagat gctagttagt
    34981 ggtttcaata accacctttt tctgactcga tgttttttat aatgaacaat tacaggatcc
    35041 gcccgtcgca caaccactat gtgatgtttg aaagaatgga ttacctgttt ctattttgac
    35101 atgttctgaa acagcgtacg caattttcgt aacaacttca tctattaaat gttgaagtgc
    35161 catttcccta gctttgtaat gcatcacaac gtcaagcatc tcatatgctc ttttgcgttg
    35221 tcttgtctct aacataactt tcttataatc aggatgatat ttcccgaatc gcattacttc
    35281 atcatatttc tcttttgatt ttaaaaatga ttgataaagt aaatgtgctt cgtcattgtt
    35341 ttcgagcgcc tgtttagctt gctcaaatga tgcataaata tctgatgcaa ctatcatatc
    35401 agctagttca tcaatatcat ctaatatcgc tagtgaagct tcattaatca taaatacccc
    35461 tcattgttat ttttctaaga aaattagtga ataataatct ctcattacat ctccacccat
    35521 ctcatcgtat tttgaattta atagacgaga tcgatgtata tctgaattca tccaactatg
    35581 gattaatgtt ggtacatcat taaacgcata accaacattt tgagcagttg ttttataagt
    35641 aacgtgattt ttatctaatt gccctcttaa tgcgtcctct gtaaattcaa cactatcaga
    35701 accattagag gtcgcttcat ataagttatt agatgcaata tgtgctaaat cgctattgat
    35761 tttcaatggt tttaatcctt ttaattttct catttcattc gttacttcat aaagagaaat
    35821 taattgattt ggattttgct caactggacg cttattatgc tcttctgacg tagaattaga
    35881 atttaattga taaggttcaa tatctgctaa catttctttt gttaaaaatc gtacacttag
    35941 cacctttttc gattgttgat cagaatacac ttgtgcatat atgtcgccat atttaatcag
    36001 tgtttgtgtt tttaaatctt catctgaaag ttcaaattca tattttttac catcaacttt
    36061 aaaggacggt tctggattaa tacttgtatg attaaaaatt tctgcagaat gttgtcctat
    36121 ttttaacgga ctaacattga ctttctcacc tgtagcatac actgaaacga tttcttcacg
    36181 tttagttgaa acaatgtaat aactgttttt gtctttaaac acataatttt tgtaaccatc
    36241 tctaaaaggg tagacacgat ctgcttgtcc aaatttagaa gtaagcactt taatatcttt
    36301 acctacccat gtaccaacac cttctttagg ctttggattt tcagcatggt tatttgattt
    36361 attcacctgt tcagatctat ttactttatt acctttattc gggttctcta atacatcaaa
    36421 ttttaatctc ggtgaataaa aaagatatat taaaaatcca actaaaaata atacaccgac
    36481 gactcttata attaactttt tcatcaatca accacctaaa aagtattaat actattgtaa
    36541 aaaacaacac attaattagc aaattttcaa cactgacata actgtgtcgt ttcgataaaa
    36601 caaaatcatc ttcaggcata attttaacgt cataaagatt ttccctacac tctatatcat
    36661 aacctatctt tatgttttca ggttgaattt cattattaag attaaaatat gtataaaaaa
    36721 atggacagtt aaggtatcaa atgaatacca tcaactgtcc gactattctt catcaaaaaa
    36781 cctgataaaa caaattgcct tatcagatta gtatcatttg tataagcata ttaatgacca
    36841 aatgttgctt taatcagtga tgtcgtttct ccacctggat ataatacata taataataag
    36901 taaactgcta cacctgtaat tgcagtacag aaccatataa ctgaagcgaa tggcccgaat
    36961 ttgcggtgta cattatattt atctttaaat gcagtaataa tttgaactag gcctagaata
    37021 ccaccaattg ttgctaaatt aatgtggaaa aataagaaaa tcgtataata tttcttaatt
    37081 gatgctggtc cgccaaatgc tgtattaccg ataaaaatcg ttctacttgc atagattaag
    37141 aaaaaagtta aagcaaaaac agctgcagct aacataacat ttttgtgttt atttatttcc
    37201 cttttccaaa taagtctcca accaatggca attaaaattg cactaatgac aatacatgtc
    37261 gtactaatcg ttggtaaaat tggaacgccc atatttttca tcctaactta attaatctag
    37321 atcaaagtaa gtaatgaaac aatcacagct aacacgaaaa agatcactaa ataatttagt
    37381 gaatatataa acatttgtgt tgcccatttt gtttgatctg aatttttctt aaatgttgtt
    37441 aaacctaatg caatccatcc taaatttaat aatgtagcta acactacgaa tacgacacct
    37501 aaatttatta gtaataaagg tactggcaat aaaataatca accagataaa catactgaca
    37561 cgtgtacgtt taaagccctt aactgatggt aacattggaa tatttgcaag tgcatattca
    37621 tctttacgtt taatagctaa ggcataaaaa tgaattggtt gccaacaaaa tacaactaaa
    37681 aacagcgcaa tcgctgttaa actaatttgt ccttcaattg caacccatcc aattagtggt
    37741 ggtactgctc caggaaaact cccaatcact gtgttccatg ttgtatgtct tttagaccat
    37801 attgagtaat aagacacata acctacaatc cccataagac caagtacgcc tgatggtata
    37861 ttcaataaaa acaaacaaat ttctccaact aacatcatac caaaacttaa tagtaataaa
    37921 ttttgatctg taattctatt atttacagtt ggtctatttt gtttactagg cataatacga
    37981 tcaatatctt ggtcgtaata attatttaac gcacatgcgc cacccataat taaagtagat
    38041 ccaaatagca ttaataaaat ttgaggtatt gatgataaga aggaatgatt tgtcattaca
    38101 actgctagcc atgcgcccgc aaaagccgga attaagttac cttgaacaag tcccatttta
    38161 attatctgtt gcaattcttt gaagttaact ctgctaatat tttgtgacaa agtatgctct
    38221 ttgctcataa tccccctcct taaatttgtt catataagat tatgatatct tagattgcat
    38281 aaaaagacta ggtttaataa aattaaattg tgacaaatta acgacaagag aaaatgtcaa
    38341 ttttgtgaca caaataacat ttaatttatt gctataatgt atatgttaga aaattttaat
    38401 aagtagaatc atgcatctaa aagagattaa tatttaagct tcaaatttga gtaaacgtgg
    38461 attacataat tatcccaata aaaaaatcat tacgattaag ttctttttat gtcgtccaca
    38521 tacaatactt gtaaaattaa atcatatttc ctgcgttgga tcccatcttt tcatatccta
    38581 caatcaggtc tatttatagt atcatctcaa atccggctat taattctaat tctcagtgat
    38641 gcgttttttt atgatggggt gtataaattg tttggcaaaa agaatttaaa atggttaggt
    38701 gtcgtagcaa cgttaatgat gacatttgta caacttggtg gagccttagt taccaaaacc
    38761 ggatcagctg atggttgtgg ttcttcttgg ccactatgtc atggtgcgtt gattccagaa
    38821 ttctttccta ttgatacgat tattgagtta agtcatagag ccgtttcagc tttgtcttta
    38881 ttaatggtct tatggttagt tatcactgca tggaaacata taggctatat taaagaaatt
    38941 aaacctttat caatcattag tgttggattc ttattattgc aagcattaat cggagctgct
    39001 gctgttattt ggcaacaaaa cgattacgtt ttagcattgc actttggtat atcattaatc
    39061 agtttctcat ctgtattttt aataacattg attattttct ctatagatca aaaatatgaa
    39121 gctgacgaat tatatatcaa aaagccatta agacgtttaa catggttaat ggcaatcatc
    39181 atttattgtg gtgtttatac tggtgcacta gtgagacatg cggatgcaag tttagcatat
    39241 ggtggttggc cattgccatt ccacgatctt gtaccacatt cagaacaaga ttgggttcaa
    39301 ctcacgcatc gtatcatggc ttttatagtg tttacgatta ttatgattac ttatattcac
    39361 gctgttaaaa attatccaaa taaccgtact gttcattatg gttatacagc tgcatttata
    39421 cttgttatat tacaagttat cacaggtgca ttatctatta tgacaaatgt taacctgata
    39481 atcgcacttt tccatgcatt atttatcact tatttatttg gtatgacaac atactttatc
    39541 atgctaatgt tacgatcagt aagaagtgac aagcaataac aaaaaagcaa accgtaattt
    39601 taatggcacg cccattaaaa ttacggtttt ttatatcaat atttaaaatt aaacctaagc
    39661 catgtaaaaa cgagattaca cgtcaattgt tgtgtaatct cgttttattt taatcatttt
    39721 agtcagttgc tttttcaatt tcgattaata aatcgcctgt cgctattgtg tcaccattat
    39781 ttacagttac ttgtttaatc acaccgtcaa atggtgcttg aattgttgtt tccattttca
    39841 tagcttcagt aattagcaac ggctgattag ctttcacagt ttcacctaca ctaaccttga
    39901 cttcagttac tgaacctggc atttgagcac cgatatgact tggattactc ttatctgctt
    39961 ttggcttaac gttcgcattt gtatgcacat tttcatcttt aatgtaaata cgtctcgctt
    40021 gaccattcat cgcatagtaa atcgttctat taccattttc atctggttca ctaatcgttt
    40081 ctagtttaat aattaatcgt ttacctttat cgatttcgat ttctactgtt tcaccattac
    40141 gcattccaaa gaagaatgta ggcgtatcaa gtaacgataa gtttccgtat tgatttctag
    40201 tttgaatata ttgttcatat acttttggat atagtacata actaataata tcttgctccg
    40261 taacaggacc ttgttgctct tcttcaagca actcacggac tttttcaaaa tcaactggct
    40321 ctagatattc acctggacga gctgttagtg cttcttggcc ttttaaaata accgcttgta
    40381 aatctttatt aaaaccattt acaggttgtc ctatttcacc tttgaagaac gacactactg
    40441 attctgggaa atctaattta tagccatctg taatcacgga ttgttcatca agatcatttt
    40501 gtaccatata aagtgccata tcaccaacta ctttagacga tggtgttact tttacgatat
    40561 caccaaataa gaaattcact ctgcgataca tatctttgac ttcatcaaat ctttcgccta
    40621 aacctaaact tttagcttgt tgacttaaat tcgaatactg tccaccaggc atttcatgtt
    40681 gataaatttc agtattcggt gatttgatat cactttcaaa gtctgaataa taagtacgta
    40741 cagttgacca ataatgacta agtgactcca taccttcaat atcagttcta aggtggcgtg
    40801 ggaagccatt taatgcataa tataacgaat tggcgcttgg ctgacttgtt aaaccactca
    40861 ttgaagcaac agcagtatca atgatatcga caccagcatc tattgcttgt ttgtatgtta
    40921 ataaaccatt accacttgta tcatgagtgt gaagatgaat tggtaaatct acagctgatt
    40981 ttaactcacc aatcaattcg taagcggctt taggttttaa taagcctgcc atatctttaa
    41041 tcgctaaaat atggaaacct tcacgttcta actctttagc tagtttgaca taatactcta
    41101 aagtataaat gtttgatcgc tcaggattta aaatgtcacc tgtataacaa atagtacctt
    41161 ctgagatttt gcccgcttct tgtactgctt cattggcaac tttcatttga tctacccagt
    41221 ttaatgaatc gaaaattcta aagacatcta tgcctgcttt agcactttct tgtacgaatt
    41281 tatgaataac attatcagga tagtttttat aaccaactgc gtttgaagca cgtaacaaca
    41341 tttggaataa tacatttgga atagctttac gtagacgttc aagtcgttcc catgggtttt
    41401 ccttcaagaa attatatgcc acatcaaatg tagcaccgcc ccacatttct agtgagaaac
    41461 catctttaaa tacgtccgct gttttggatg cgatattaat catatcctta gttctaactc
    41521 gtgtagctaa taatgattgg tgtgcatctc taaaggttgt atctgttagt aagacatcat
    41581 cctgcttttt aacccattca gctacacctt ttggacctac ttcatcaagc aattgtttcg
    41641 taccactaaa tgaagcgatt ttacttgaag atacagttgg aattgatgct aattcatagt
    41701 ctggtttcgg acgtttctca acatttggga aaccattaat tgttacatta cctatatatt
    41761 ctaatgtttt agtacctcta tctagagacg gctgaatgtt gaaaagttct ggtgtttctt
    41821 caataaattt agttgtgtaa tcaccacttg tgaacttttt attcttcatt acattaatta
    41881 agaatggaat attagtttta acaccacgaa tacgcatttc tcgtaatgag cgtaccattt
    41941 tttcttctgc ttgtttaaat gatatcgcgt gtgtagataa ttttacgagt aatgaatcat
    42001 aataaggtga tatctcagca ccttggaaac catctccagc atcaagacgt acaccaaagc
    42061 ccccgcttga acgataagca atgattgttc cagtatccgg catgaaatca tttaacggat
    42121 cttctgttgt aatacgacat tggatggcat agcctaatgt tgtaatatct ttttgttgcg
    42181 gcatattaat ctcttcacca aataaatcgg cacctgctgc aactaaaatt tgtgtcttaa
    42241 caatatcaat tcctgttacc atctctgtaa ttgtatgctc tacttgtaca cgagggttaa
    42301 cttctataaa gaagaattcg tcaccagata ctagaaattc aacagtacct gcattgacat
    42361 atttaatatt ttccatcaat tgaattgcag catcacaaat acgttgacgt aatgttgatg
    42421 ataatccaac tgatggtgca acttctacaa ctttttgatg acgacgttgt actgaacaat
    42481 cacgttcaaa taagtgtacg atatttccat gttcgtcacc tatgacttgt acttcaatat
    42541 gctttggatt atcaatgtat ctttctatgt aaacttcact attaccaaat gatttttcag
    42601 cttctgattt tgctctatgg aaagcatctt ctaattcact ttcttcacga acgattctca
    42661 tacctttacc gccgccacca cttgtggctt taatcattag cgggaaacca gcttcttctg
    42721 caaattcttt tgctaattca tatgatttaa ttggaccgtc tgtaccagga ataactggta
    42781 aatctgcctt gatagccgtt gtacgagctt taactttatc tccaaacata tctaaatgtt
    42841 ctaaatgagg accaataaat ttaattcctt cttctgcaca acgacgcgca aattgttcat
    42901 tttcacttaa aaatccatag ccaggatgaa tcgcatccac attcgcttgt tttgctacat
    42961 caatgatacg ctcaatattt aaataacttt cagcaggacc taaatcactt ccaactaaat
    43021 aggattcatc tgctttatat ctatgtaatg aacttttgtc ttcattcgaa taaattgcaa
    43081 ctgtgctgat gtctaattct gccgccgctc tgaatatacg aattgcaatt tctccacggt
    43141 tagcaacaag taactttttt atttgtttca atagcgatac actcctcaaa ctattagaat
    43201 tttctaacta attagataat aaaattttat cttaaagcgc tctgttttgc tatagttatg
    43261 tttcaaattt tcaaaattta acattcttga gacaattaaa acctccgctt cagaaataat
    43321 aatttcaaaa atgactatgc aacaacaggt agttcacgtt tttgttgtga aacattttcg
    43381 atttctacaa ctctaaaaaa ttaaaaataa aattgcaaaa catcaacatt tattatcaat
    43441 agcgataact ttatcttatc atcatgattc taatttcgcc accacattta gtaattttta
    43501 gtcataaaat ttagttataa ttatacgttg ttttgtttat aaaatttgat aatcatgagt
    43561 aatctcgtaa tatcaaaaca aaaaggaagt taagcgttgt ttggttgcct aacttccgtt
    43621 attgaactca tcagtccaat ttgacaaaga gtatataaat agccaatgtt tagcattgct
    43681 taatgacaga agtcatctcg tactaaatat tggctagtat ttttttaatt aaattgtctt
    43741 cttatatcaa ctttttgttg ttgtttcttt cgttgctggt ctactttgat ttgtttacct
    43801 acaatcagaa gtaaacccat agcaatactt aaactaatca ttgatgatcc accaaagctg
    43861 ataaatggca atggcacacc agttaatgga attgttgccg aaataccgcc aatgtttaca
    43921 aacgtttgac ttccaaagta tgtggcaatc ccaacacaca caagtttata aaaatatgat
    43981 gatgttttat ttgcaaactg gaaggcacga tatacaataa agaactctaa agtaataact
    44041 agcaatcctc cgattaaacc taattcttcg caaataattg caaaaataaa atctgtatgt
    44101 ggttctggta aatagcccaa tttcattgca ctatttccta atccttttcc aaatacgcca
    44161 ccgttaccta tcgcaagcaa tgaattggaa atatggtatc cagttcctga ttcgaattgg
    44221 aatggatctg ttagcgtact aaatctggca gttaaataac ttggtaacca accagccatt
    44281 aatgcaatga caaatactac taagaatcct agcactgctg gtataccaaa tcttaggact
    44341 ttgtttaccc caattcctga ataaaaaatg atcgcaacta aaataattaa tattagtaat
    44401 gtttgcccta cgtctttttg taggaaaact aaaaacgtac aacctaatgc taatacaata
    44461 ggacttaaaa ttaattttgg tttacttaat actctaggca tttttttact gatcataaat
    44521 gggatatata atatgattgc aatttttaat aactcagatg cctgtaagtt cataaatcct
    44581 aagtttatcc aacttttaga accattaata tctttaccta ttactaacgt cagtaataat
    44641 agtgagacga tagttataat catacctttt tgcactttaa tattactcag taatttaaca
    44701 ttcattaaaa atgcaataaa aaatacaatt ataaaactca ttatgacata tgctaattgt
    44761 cggttgtaaa aatacgttcc tggaacatcg ataccaccag tcaatgtgcc tttagttgct
    44821 ggaaccatac ttgcactata taccatcact aatccaatta aactcaatac aatatatgta
    44881 actaataacg gataatcaat aaactttgag gttttaccaa tataccgtaa aatacttcta
    44941 aaattcttca tatattcagt catccaattc tacaatttat acttcaaatg atttcgtttt
    45001 atactttaat tttaaaaatc catgttactt attatataca tgacacagtc ataatcacaa
    45061 atattaacag gtctcttgag gtaatttata gaatatttgt ttacttaata tcgtgaattg
    45121 ctaaaaccca ataaaagtga ctgacatgtc atcaagaaga caacataaca gccactttaa
    45181 tcagtaatta aactataaat ataaaatcgg cgcagctcaa ttgtcgatat ctcacgccat
    45241 tttaatatgc ttatatatta aaccttaaac aagtgtaaaa gcttcatgta attttgaaag
    45301 ttctttctca agacgtaaca taatttgttt gccatcttcg cggtcaacta aacctaattt
    45361 aacagcaaaa tcaacttctt tttgaagtcc aaacatttgt gtatctagta cttcttcata
    45421 taatgggcat gaaggtaatg ttaaattatc tagttgaact ttaatcagat gcaagatttc
    45481 atcagcatct ttagtcaatt gtttcaaagc tgcatttttc attgttgctt gtttcgccat
    45541 gacacgctcc ccctctatat aaattctcaa gtcttactaa aagtttatct tactaaatat
    45601 taaaaagcaa gtagatttaa aatgtgtcgt tagcaatttg tgttaaaata atgctatata
    45661 cattgatagg gagtgggaaa atgatacaaa ttaaaggagc cgtaaagttt cctataacat
    45721 tagatagtac gacttggatt ttcgatgata gaaaagttag catcgaagat ttagaaaaag
    45781 gtgtatttga tggttcaaaa ccaatcgact ttgaagataa tcatgaatgg aatcgtgcta
    45841 tcctagaagg acaaactaat ccgcctactt taaattcaga aataaaatat aaaaaacgtg
    45901 ccatgttaga aggttcattt gtcattaata tgacaccatt tttcaaacat tccgaaccac
    45961 atgactctgc aactactatt cgattatcaa atgagcatga tcatttagat ataccgatag
    46021 atctattacc ctatttattt ttccaatttg caaaagacgg taaacgactt tacagcgata
    46081 atggtgtaga tagttttatt tacaatcaag aagatggtta ttcatatcga atcaaccatg
    46141 taactcatat tgaggtgatt taatttgaga caagtgcaat gcattatctg tgatgcaaaa
    46201 gtatttatag atgagcgcac aactgagtct aaacgactta aaaacaatcc tattcgaaca
    46261 tttatgtgtg atgattgtaa aagtcgatta gacacaccta aacaacgtgc gcaacactat
    46321 ccgctcgatt aatatctgaa taaacaagtt agaaatttga aaatgtgaaa gtaacaggaa
    46381 ataatagcat ttaaacactt tttcaaatat tatatgccaa aagagaagct aagcaacttt
    46441 catcgctcag cttctctttt attatgttaa attattcgtt ttcttgcatc atttgtttga
    46501 tacgctttgc ttctttttca cgaacatttt tgtttaaaat tttctttctt aaacgtatac
    46561 tttctggtgt aacctcaaca agttcatcat cattaatgaa ttgtaacgct tcttccaatg
    46621 ttagaatacg cggtctattc attgtttgtg tttggtcttt cgttgcagaa cgtacgttag
    46681 tttgatgttt tgttttagtg atgttaacag ttaaatcatt ttcacgatta tgttcaccaa
    46741 caatcatacc ttcataaact tcagtaccag gttccatgaa gtttacacct ctatcttcaa
    46801 gtcccaaaat ggcataagta cttgcagaac cttgatccat tgaaattaat gcaccattac
    46861 gacgaccgcc aatttgtgct ttaatacgtg gtctaaattc ttcaaatgta tggttaataa
    46921 taccgtaacc tcttgtcatt gacataaatt cagtcgtata accaatcata ccacgagccg
    46981 gtacattaaa gattaaacgt gtaagtccat tatcagttgt agtcatatca accatttcac
    47041 ctttacgtgc acctaatgat tcaataacag cacctgcatt ttcttgtggc acttcacatt
    47101 gcacacgttc aaatggttca cacattacac catctatttc tttaataatt acttgtggtt
    47161 ttgaaacttg taattcataa ccttcacgac gcatattttc aataaggatt gataaatgca
    47221 attcaccgcg accagcaact acccatgtat ctggagaatc tgtgttagaa actttcaaag
    47281 atacatctgt ttctaattgt tgatttaaac gttcttgaat ttgacgtgct gttacaaagt
    47341 caccttcacg gccagcaaat ggagaattgt taactttaaa tgtcatttca agagtaggct
    47401 catcaatacg tagaactggc aatgcttctt gatggtcatg tggtgttaca gtttcaccaa
    47461 cattaatgtc ttccatacct gaaacagcaa ttaaatctcc agcttgtgct tcttcaattt
    47521 ctaaacgttt taatccaaag taaccaaaga ttttagttac acggaagttt ttcactgtac
    47581 cgtctaattt aattagtgat acattatctc cgacacgcat tttacctctg aatacacgac
    47641 caataccaat acgtccaaca taatcattgt agtccaacaa tgctacttgg aattgtaatg
    47701 gctcatcact gttatcaatt ggagctggta cataatcaat aattgtttca tataatgatt
    47761 gtaaattatc atcttgcttt tcaggatcta agctagctgt accatttact gctgaagcat
    47821 aaacaacagg gaattctaat tgttcatcgt ttgcttctaa ttcaataaat aaatctaaaa
    47881 cttcatctac aacaccctct ggacgtgctg atggtttatc aattttatta acaacaacaa
    47941 caggtttcag gttttgttct agcgcttttt taagtacaaa acgtgtttga ggcattgtac
    48001 cttcatacgc atctactact aagacaaccc catcaaccat tttcataata cgttctactt
    48061 ctccaccaaa gtctgcatgt cctggtgtat ccaaaatatt aatacgtgta cctttataat
    48121 caacagccgt atttttggct agaatcgtaa ttccacgctc tctttcgata tcgttagagt
    48181 ccattgcacg ttcatcgaca tgttcatttt ctctgaatat accagattgt tttaacaact
    48241 catctactaa agttgtttta ccatggtcaa cgtgagcaat aattgctata ttgcggacat
    48301 cttctctttt attagtcata taaatagttt ccttctttct taagtaaaat cccactttta
    48361 attacaactt ttttattata tcatataatt gagaaaagat aagcaaaagg tggggatagg
    48421 atgaaacaaa aaaaatctaa aaatatcttt tgggtatttt ctatattagc tgttgttttc
    48481 ttagtattat ttagttttgc tgttggtgca tcaaatgtac caatgatgat tttaacattt
    48541 atattactcg ttgcaacctt tggaattgga tttactacaa agaaaaaata tcgagaaaac
    48601 gattggctat aagttatatc gacaataaca cgcgttgatt taagaccaca catgttcatg
    48661 atgaacattt aactggtttt atattggcgc gtgtttttat tatttaaggt ataaaagcgt
    48721 aatatgttac ctaattgtta ggactacata ttacgctttt ggtaattatt ttgattttct
    48781 tttaaatcgt tgttcatgta attgtattaa cgcatcatgg tggggctcta aataatcatt
    48841 gctaatttct tgatggagac cacgatttcc aactaagatt gaatttggac cactaattgt
    48901 taatggttct cctagtaaat tggaagcttg tccatttact tcatataaaa taaccaatcc
    48961 gccagcaaaa tcccatggtt gaagtcttgg cgtcatatat gcttctaaat tacctgtagc
    49021 aactgaaacg atttcaagcg ctgcactacc atatgccctt gcacttctag aatcattaac
    49081 aatttcttta aagatttctc ctaaaattgg tttagttaac cagttcggat tgatcccaat
    49141 aatgctttgt cttagattag aatcattcaa tggtttcaag ggttggctac cacgatatgc
    49201 accttcccct actttagcat gatataagac atcagccata acatcatata caaaacctgc
    49261 ataaggttta ccatcgatat aaataccaat tgaaattgcg aaattttctt gttgatgaac
    49321 aaaattcaat gtaccgtcta ttgggtcaac aatccatacc gtacctttgg aagtatcgat
    49381 gtcatgacca tgcccttctt cacctaatac ttgatgattg ggatatgttt ctaaaattgt
    49441 atcaaaaatg aaatcttctg ttgctttatc tacatttgta acaaggtcat tcggatttga
    49501 ctttgtttca attgttaaat tttgctccat caattgttta attctaattc ctgcttcttg
    49561 aataagtcct tgggcaaatc cataaagtgc cataacatca tctcctaaca tctttattat
    49621 acatcaacat tttataaaaa taacttctta tgataaaaat gaaaatacta attttaatga
    49681 gagcgtctta agccgcaatt gataaaacat atgctacaat atttttaaac actatacagg
    49741 aggtactcta tgacaaaata tacatttaaa cctaaagatt tcaaagcgtt caacgtagaa
    49801 ggcttagacg cacgaatgga agctttaaac gaatacatac gaccacaact ccgtgaatta
    49861 ggagaatatt ttagtgactt ctttacaagt caaactggtg aaacatttta tcctcacgta
    49921 gcaaagcatg ctagaagaag tgtgaatcct cctaaagata catgggttgc ttttgcaaca
    49981 aacaaaagag gctataaaat gttacctcat ttccaaattg gtatgtttga agatcaactg
    50041 tttgttatgt ttggaatcat gcatgaagca aaagataaag caacacgtgc aaaagttttt
    50101 gaaagaaaat ttaaagctat tcaacaatta cctgatgatt atcgtgtttg cttagatcat
    50161 atgaaacctg ataaaccatt tattaaagat ttaacggatg atgatttaat agaagcgata
    50221 caaagagcca tcaatgtgaa aaaaggtgaa ttctttatag cgcgtgcaat cacaccacaa
    50281 gataaaagat taaaaagtga caaagcattt attgcatttt tagaagaaac cttcgatcag
    50341 ttcttaccat tttattctgc ataaataact ttgtttaaat aatagagcac ttaatcacat
    50401 ccatgatttc gtgctctttt ttcttaatat taaatcgaac gttcaacata ataattcata
    50461 cttttaaaaa aattaaaata aatttaggtt gacctaaaca ttttattagg ttattatatt
    50521 gtccataaga agtagaggtg agtcaaaatg aataataaac gacattcaac aaatgaacaa
    50581 ttaagtttag acgaaataaa caatacaatt aaattcgatc atcgcagttc aaataaacag
    50641 aaatttttat catttcttgg acctgggtta ttagtcgctg ttggttacat ggatcccgga
    50701 aactggataa catcaatgca aggtggcgcc caatatggct atactttgct attcgtaatt
    50761 cttatttcaa gtttatcagc aatgttactt caaagcatga cagtgagatt aggaatagca
    50821 acaggtatgg acttagctca aatgacacga cattatttat caagacctat tgctataatc
    50881 ttttggatca ttgcagaact agcaattatc gctacagata ttgctgaggt tattggtagt
    50941 gctattgctc ttaatctcct atttaacata cctttaatcg tcggtgcact aataactgta
    51001 cttgatgtat ttttactact ttttataatg aaatatggtt ttagaaaaat tgaagctatt
    51061 gttggtacat taattttcac agtgttattc atctttatat ttgaagtcta tatttcatca
    51121 ccacagttga atgctgtgtt aaatggattt ataccacata gtgaaatcat tacaaataac
    51181 ggcattctct atattgcatt aggtattatt ggcgctacaa ttatgcctca taatttgtac
    51241 ttacattcat caattgtaca atctagaaca tactcaagac ataacaatga agaaaaagcg
    51301 caagcgatta aatttgctac gatagattcg aacattcagt tatcaatcgc atttgtagtc
    51361 aattgcttat tattagtgtt aggagcatca ctatttttca actcaaatgc tgacgattta
    51421 ggtggtttct atgatttata tcacgcctta aaaactgaac ctgtactagg tgcaacaatg
    51481 ggtgcaatca tgagtacatt atttgcagtt gcattattag cgtcaggtca aaattcaacg
    51541 attactggta ctttagcagg acaaattgta atggaaggat ttttaagatt acacatacca
    51601 aattggttaa gacgtttaat tacacgttct cttgctgtca ttcctgttat cgtatgctta
    51661 atcattttta aaggtaatgc agctaaaatt gagcaactac ttgtattctc tcaagtattt
    51721 ttaagtattg ctctgccatt ctgtttaata ccattacaat tggctactag taataaagac
    51781 ttgatgggac ctttctataa taaaacatgg gttaatatca tttcatggac acttattatt
    51841 attctaagta ttttaaatgt ctatttaatc gttcaaacat tccaagaact acaaagttaa
    51901 aaataaaacc attaaaatgt tggttgtatc taataattaa caacttaaca ttttaatggt
    51961 ttttcttttg ctatgaacat attaatgata aatatactca tcgtccttat gtgttgtttc
    52021 ttttgattct tttttagaaa tcaacataat catcacgata acccataaga caattgcaat
    52081 taaattaaga gccatcaaac ttacaattat cgcgataaca aaaagtataa tcgataattt
    52141 tctacgtcct ttaatacaaa ttgtcgcaaa tataccataa agtgttgaaa ttaataaaat
    52201 aattgctaat gcacccattg ttgtctgaag catatctaaa gaaaccttgc caccgttctg
    52261 agataattgc ttcgctaatt cttcacgttg tgatgcatta ccacttttta aagaaaaata
    52321 ggatagcccc ataactaata aataaataat actaatacta tttgcaatcc aagccatgat
    52381 taattcaggt tttctattca tcttaaaatc ctttctttgt ttaatatttt aataagtata
    52441 ctacgagcct atagatatac gcacattaaa ttacttgtat attaacatat ttttcatcta
    52501 atttttataa taaacatctg aattcagatt gatgataatt taggtgtata gtaatgagaa
    52561 atcgtcattt aaaattatga cacctaaaca ctttaattaa acgttatcga taattaacct
    52621 catataaaaa cccagattaa taagcaaaag acctctgctt actaatctgg gtatcatcaa
    52681 tatttattct tgctcttttt tgtccttaac ttcacttgaa ttgcgttctt cagaaatatc
    52741 attgttgtaa atttgacgtc gctcttcaaa attcattcga cgttgactaa ctgcgctagg
    52801 ttgatttttc ctacgttctt tcattcgttg gttataagct ttacgttttt cttttttctt
    52861 agctttaatt tcagcttttt ctcgcttttg ttgtgctttc aattcttttg gatcaacttc
    52921 aggttctgca taataaccat catcttcaat acctagttct tcagtacttt tttgtttgta
    52981 tttagcctgt ctggataata ctgatggttc ttcctcagtg cgctcatcat gattatattc
    53041 actttcaact gcacgtttag gaaattgatc atatttatct ctttcctgtt cttgatcata
    53101 tacagtattt gatttatcca attctttttc atatttaggc acatcataat aatcatcttt
    53161 cggttggggg cgctcataca caggttcacg ataatcataa atcggtccgt tatactcttc
    53221 ataatattgt ggcgcaacca tttcaacttt atcttttctt ataaatagca tcatcgcaat
    53281 aataaagaat aatatcggaa tgattaaagt aacgataagt aaaactaaag gaatcgttac
    53341 aattgctgat attaagaata agaaacctga caagattctt atattcatag atatgagagc
    53401 taaaaatgaa atcaacaaac aaacaataaa gtaaacgata atagcccata taccattttg
    53461 taaccatatt acaaattgtg ttgtattata gccatttcca gctaataatt gctggataaa
    53521 tgcattattg tttaatgtat tttcaagatt agcaatcgat gtgttattac tgaatgaaac
    53581 aagtgctata aacatcgtaa tgacagtaag cactaataac atcacccaac ataaccaacc
    53641 tagaactttt tcagttaatc tacttactgg acgtttaatt tgagtaaatt gttctccagt
    53701 cattcgttac aactccttat agtacttatc ccgttattat aactaaatat acagtaaata
    53761 actactattt atgattttat tttaatgaca ttttgaaatt caaaaagttt tcattgtatt
    53821 cacttaaaac ttcaggtcct aaatctttat aaacttcaag gcgttcttgc tctttcttag
    53881 tcggataaaa acgatggtcg tctttaatct ctttaggcaa caattgtcga gcagccttgt
    53941 ttggcgttgc atagcctacg aattctgtat tttgcttgtt atttttagca tctaataaaa
    54001 aattcataaa tttatatgca ccctctttat tttgtgccgt ttttggaatt accatattgt
    54061 cgaaccataa attcgatcct tctttaggaa taacataatt atatttatcc ccttcttgca
    54121 ctagaggtgc tgcaacacca ctccaaacaa ccgctatgtt accttcattt tgttgaagca
    54181 tcatggtaat ttcatcacct acgacacctc ttacttgtgg tgctagtttg gttaaatctc
    54241 gctctgcttc ttttaaatgg tgcgaattac ggtcattaag attataccca agtttattca
    54301 aactcatgcc tataatctct ctagcaccgt caactagtaa aatttggttt ttaaatttag
    54361 gattatacaa tgacttccaa ctatcaaatg attcatttgg atacttttct ttattatata
    54421 aaatacctac agttccaaag aaataaggta aagaatattt attgcctcta tcaaatgaca
    54481 tattcatata atctgaatct aaatttttaa tattaggtac cttattatga tctattggta
    54541 acaataaatg atctcttttc aatttttgaa ctgtatattc actaggaaaa gcaacatcat
    54601 aatgtgtacc gccattgcga attttggctt ccatcgcttc atttgaatcg aaagtttcat
    54661 aaacgacttg aatgccagtt tctttttcaa atttcttaat taactctgga tcaatatatt
    54721 cgccccaatt gtatacgtaa attttttgat ttgtatgcac ttgttcttta gatttaaacc
    54781 aatgacttaa agtaagacaa agcataccca caactaatgc acctataatg agttgtaaaa
    54841 atcgtttcat tattttacac ctcgcttgat tagttttttc ttatttatca cgtattgaat
    54901 caaataatat cctagtattc ctaatacaat aacagcaaac aataatgttg aaatcgcatt
    54961 aatttccata ctaattcctt ttctcgccat agcataaact tcaactgata acacactaaa
    55021 gccattacca gtaacgaaga aacttactgt gaaatcgtct agtgaataag ttaaagccat
    55081 aaagaatcct cctataatag aaggtaaaat attaggaata ataatgttgc ttaataattg
    55141 tggttcagtc gctcctaaat ctcttgcagc atttaacata ttattattca tttcatacag
    55201 ttgtggtaag acgataatca caactatagg tatgcaaaat gcaatatgag atattagaac
    55261 tgtccaaaat cctaaaccaa gaccagtaaa atggccaatc gttgtaaaca taattaagaa
    55321 tgatgcacct atgacaacgt cggatgatac catcaagaca ttattcaatg ttagtaaagt
    55381 tactttaaac tttttatttc ttaaataata aatagcaatg gcaccaaatg taccaataac
    55441 tgtagaaatt gaggctgcta aaagtgctac agctatcgta ttaaaaatga ccgacattaa
    55501 atgatcattt tgaaataatg attgataatg ctctaatgta aaatgttcaa agtgaatcat
    55561 attaccagcc gaattgaatg aatagaacat taaaaagaat attgggatgt ataaaatcgc
    55621 taaaagtatc ccgatataca gctttccata ccatttcata tgattcaccc tctcccatta
    55681 gatgattttg taatgattaa aatgaatgcc ataaatacaa ttaagaatat agctatagtt
    55741 gatcccatac cataattttg aattgttaaa aattgttcct ctattgccgt acctatattt
    55801 atgactttat tacctgcaat taatcttgta atcataaata atgaaagtga tggaataaag
    55861 gttacttgaa tcccagtcat aacaccttct tttgttaacg gcatgattac ttttctaaaa
    55921 gtatagaaag gactggcacc taaatcactt gaggcctgca ataaattatt aggaattgct
    55981 ttcatgctat taaatatagg taaaatcata aatggtatat aaatgtaact tgccactact
    56041 aaaaacgcac cagttgtaaa taacaaattg aatgatggta aattaaataa gtggaaaaat
    56101 tgattaatca cgccatcatg acttaataaa cctataaaag catatgtctt taacaataaa
    56161 tttatccatg ttggaataat cattatcatt aataagatat tttgaaattt cgaacgagta
    56221 atataatagg cagctggata actgatagtc aaggtaataa ttgttattga agcggcatat
    56281 aaaattgaat atgcaaacat tttcaaatat tttgtagtaa aaatttgttg ataattcgtg
    56341 aaactaaaat gtccattgat atctaaaaat gaaaaataaa tgagtaatac aactggtaca
    56401 ataataaata taaccatcca tagtaaatac gggattaaga gaaatttatt agtattacgc
    56461 attgtctact tcctcatagc tttcaatacg tttatcaaat tcttcttctg tttctccagg
    56521 aaccataata tgaatggctt caggatcaaa ataaagacca acttcactgc ctacttcagc
    56581 ttttttagtc gtttgtatta cccattcata acctttattg tctatacaac atatttcata
    56641 gtggacccct ctaaataaca tagaatcaac agttgcttta aataatcctt cttcagcttt
    56701 gattaatgat atatcttctg gtcgaataac gacttctact tttttatttt caggaatacc
    56761 catatcgaca cattcgaaat cttgcccata aatattcacg acataatctc taaccatgcg
    56821 cccttcaaca atattagatt ctccaataaa atcagctaca aatcgattca ctggttcgtc
    56881 atatatatct gttggtgtgc caaattgttg aattttccca tcttttaata caaaaagaaa
    56941 gtcacttaat gctaacgctt cttcttgatc atgtgttaca aatataaatg taatacctaa
    57001 tctagattgc aattctcgta attcatattg catttcagta cgcaatttca aatctaatgc
    57061 ggataaagat tcatctaaca ataatatttc tggttcattt acgatagcac gtgcaattgc
    57121 aacacgttgc ttttgtccgc cactcatttc attaatattt cttttttcat aacctgaaag
    57181 ttttactaat tttaatgcct cagttacttt ttgatcaatt tcggtttttg ataatttttt
    57241 taatttcaaa ccaaaagcga tattatcata gacgtttaag tgtggaaata atgcataatc
    57301 ttgaaagact gtattcactt tacgtttatt tggtggtaaa ttaccaattg gtttgttttg
    57361 ataaatcact tcaccactgt caggatattc aaaccctgca attaatttta aaattgttgt
    57421 tttaccacaa cctgaaggac ctaataatgt ataaaagtat cctgattcaa tatcaatatc
    57481 tatgtcatct aagatattaa gatcatcata gcttttacta actgatttta atgataataa
    57541 cggttccaaa taagatcccc cctataaata tgaagctgtc gctacaataa gtattcgtgt
    57601 ttcattgttt gattcgtttg acaaacgatg attatctcgt gctttaaaat acaaaacgtc
    57661 ttcttcttgt gcttgatata tctctttgcc taaattaagt gttatctgac ctgacataca
    57721 ataaataaac gtatcagact ctgatggatt aaaattttta tatgatgctc caggctttaa
    57781 agttaatatt aatggctcca tatcatattc atttgacttt gaaactaacc aatttaatat
    57841 ataaccttca tcatactcat cataaataac ttgttcttcc ttcttgtata atactttttc
    57901 attttcactg tctttaaaaa attcacttgg cgtcgttcct aacacctcta taatatttaa
    57961 gaaagtttcc atacttggtg aggcatgttc actttctatt tgtgaaatgt agcctttcga
    58021 taagtctgta cgttcagcaa gttcttcttg cgttaaattt ttaattcttc taagattttt
    58081 aattttatta cctatgttca tttatttttc cacctaaaaa agctatatta agtttacttt
    58141 tatcaaacat tttgtttaac acttgataaa ggtatcaaaa tatagcttta ttagcaatag
    58201 ttttaaagaa aaattcctat taaattgtgt taatttcgaa cgaattattt tttagctgtt
    58261 actctttgtt cttcagagtg agtttttaca tcttgaactg ctttgtaact attatagcca
    58321 ctactttttt cgaaagtatt aaaaatttgt ttttcctctg ctttagcagg cacaatttct
    58381 ttaaatcttt tatatgcacc cataaagtcg cctgccgtca cgccggattc ataatacttc
    58441 tctacatgat taaagaaatt tatcactgaa atcatctctt cattactcca gtctaaatca
    58501 attggatact catattccat tgttgaaccc tccatttcaa ttcttttgtt agcatcgcta
    58561 gtataacatg catgtcacat gttaacgata tctcaaatca atttccttac attactttac
    58621 ctaaaacaaa aaagccgtag catatgctac agcttttaat gactgaatct ttatagataa
    58681 tcagttatta cattgtatgg attgggtatc cgatagcttt ttctgctgct tccatagtca
    58741 tctcacctaa tgttggatgt gcatggattg ttaatgcgat atcttcagca ttcataccag
    58801 cttcaattgc taaacctaat tcagagataa tatctgatgc accagtacca actacttgag
    58861 caccgattaa agtatcatct tctttaagtg taataagttt aacaaatccg ttagtatcat
    58921 ctaatgataa tgcacgacca tttgctgcat atgggaattt agaagcttta attgctaaac
    58981 cttcttcttt agcttgcgct tctgaataac caactgtagc taattctggt tcagtaaagc
    59041 atactgctgg cataccaatg taatcaactt cagcagcttg accatcaatt gcttcagcag
    59101 caactttagc ttcatagcta gctttgtgag caagtggtaa acctggaacg atatcaccaa
    59161 ttgcatagat attgctgata gacgtacggc tttgtttatc aacttctaat aatccacggt
    59221 cagcgaattt aacacctaat tcttctaggc ctaattcgtc tgtgtttgga cgacgaccta
    59281 cagttactaa tacataatca gcttcgattg ttttctcttc gcctttagct tcataagtaa
    59341 ctttaactcc gttatctgtt tcttcagctg atttagccat agcttcagta acgatttcaa
    59401 cacctttttc tttcatacct tttttaacag gttgtgtcat ttgtttttcg aagccaccta
    59461 agatatcttt agcaccttca aggatggtta cttctgaacc aaagttagca aatgctgtac
    59521 ctaattctga tccaatgtat cctccaccaa ctacaactaa tttacctggt acttcttgta
    59581 agtttaaagc acctgttgag tcgataacac gtttaccgaa tttgaaatta ggaatttcaa
    59641 ttggtcttga acctgttgca atgattccat ttttaaagtt gtatgtttgt gcgctctttt
    59701 cgtccataac acgtaagcta ttgttatcta cgaaatatgc ttcaccttta acgatgttaa
    59761 ctttgttacc tttaagtaag ccttcaacac caccagttaa tttattaaca actgatgatt
    59821 tgaattcttg aactttttgg aagtttaaag aaacactttc agcaataaca cctaagtttt
    59881 cagaatgttg tgcttcaaca aaacggtgag aagcatgtag taatgctttt gaaggaatac
    59941 atcctacgtt taagcaaaca ccaccaagat tacctttctc aacgattgtt actttttgtc
    60001 ctaattgagc tgcacgaatt gctgcaacgt atccaccagg acctgctccg attactatag
    60061 tatctgtttc aattgggaaa tctccaacta ccatgtttta cccctccatt aataataatt
    60121 ctggattatt taataaacgt ttaatgtgat tcattgcatt ttggccagtt gcaccatcaa
    60181 tttgtctgtg gtcaaagctt aatgataatg ctaatactgg tgctgcaaca atttctccat
    60241 ctttaacgat aggtttttga gcaatacggc caattcctaa gattgctact tctgggtgat
    60301 tgataactgg agtgaaccat tgtccaccag ctgaaccgat attactgatt gtgcatgtag
    60361 cacctttcat ttcatcggct gttaatttac catcacgtgc tttaacagct aattcattaa
    60421 tttcatctga aatttggaaa atagacttac gatcagcatg tttaacaaca ggtactaata
    60481 atcctctatc agtgtctgct gcaataccga tattccagta atgtttatga acgatttcac
    60541 cagcttcttc attgaatgaa gtgttaagtg ctgggtattt tttcaatgca gaaacaagtg
    60601 ctttaacaac ataaggtaag aatgttaact tagtaccttg ttcagctgcg atttctttaa
    60661 atttcttacg gtgatcccat aatgcttgaa catcaatttc atccattaat gttacatgag
    60721 gtgcagtatg cttagagtta accattgctt tcgcaattgc tctacgcata gcagggattt
    60781 tttcagttgt ttctgggaag tcgccttcta atgttactgc tgcaggtgct gcaggagttt
    60841 cagcaacttc ttcacttgta gctgaagcag ctgattcatt tgaagctgtt ggtgcaccac
    60901 catttaagta tgcatctaca tcttcttttg taatacgacc atttttacca gatccagaaa
    60961 ctgctttaat gttaacacct ttttcacgtg cgtatttacg tactgaaggc attgctttaa
    61021 cagttctgtt ttcatctact tcttcagttt gagtagctac aggtgcttgc tctgctggcg
    61081 cttcctcttt cgcaggttct tctttagatg atgaatcatc atcatgacct ttaaattgca
    61141 tatcttctgc atcaggtgca tcgattttaa caataacgtc accaactaca gctactgtac
    61201 cttcttctac cataacttct tctacagtac cagatactgg tgatgggatt tctactactg
    61261 atttatcgtt ttgtacctca gctaaaacat cgtcttcttc aatagtatct ccagctttaa
    61321 caaaccattt tacaatttca ccttcgtgga taccttcccc gatatcgggt aatctaaatt
    61381 caaatgccac gtttttgccc tcctaagatt tcgtaaatta aacatttcat tttaatcaat
    61441 gagactaaaa tacgctaact tcgttaactt ttaaaatgta ttaaaattct aaagtttctt
    61501 ttgctttttc gatgatgtca tttttgtttg gtaaccaaac attttcagct tgagtgaatg
    61561 gataaattgt atctgctgct gcaactcttc caataggtgc ttctaatgaa aggattgcac
    61621 gttcacttaa ttcagctaca actgctgcac caacaccagc ttgacgttgt gcttcttgaa
    61681 ctacaactgc acgaccagtt ttttcaactg aagctacaat tgtgtcaaca tcgattggtt
    61741 gaacagtacg taagtcaatt acttcaacag aataaccatc tttttcaagt tcttctgcag
    61801 ctttcattga ttcttgaacc atcgcaccgt atgtgatgat tgaaatgtca ttaccttctt
    61861 ttttcacatt agccttacca atgtcaattg tatattcttc ttcaggtact tcttcacgga
    61921 atgaacgata caatttcata tgctctaagt atacgactgg gtcattactt ctaatagaag
    61981 aaattaataa acctttcgca tcgtatgggc ctgaaggaat aacaaccttt agacctggag
    62041 attgagctaa aataccttct aagttatctg cgtgtaattc tggtgtgtgt acgccaccac
    62101 caaatgggct acgaattgtt acaggtgcag ttttagtacc gcctgaacgg aaacgagtac
    62161 gtgcaatttg tccagcaatc gcatcaaata cttcgaatac gaaacctaag aattgtactt
    62221 ccataaccgg acggaatcct tcaactgcaa gacccatcgc taaaccacca atacctgatt
    62281 cagctaaagg tgtatcgaat actctatctt caccaaattc tttttgtagt ccttcagtaa
    62341 cacggaaaac accgccgtta acaccaacgt cttcaccaaa aattaaaaca tcttggtcat
    62401 ttttaagttc agttttaagc gcatcattaa tcgcttgaac cattgtcatt tgtgccatgg
    62461 cttacttcga ctccttctct ttgtaaattt catattgttc tgctaagttt tgaggcatat
    62521 cttcatacat aatttccatt agagaagtaa cagtttgttt ttcagtgtta tcagcctctt
    62581 taattgctgc tttaatatca gcttttgcac gttcaataac ttcattttct ttgtcttcat
    62641 tccataaacc tttgttttca aggaatttac ggaaacgtac taatgggtct tttttctccc
    62701 attcagcatc ttcgtctgaa gttctgtaac gagttggatc gtcaccagcc attgtatgag
    62761 gaccataacg atatgtcata gtttcaatta atgttggacc ttcacctgca actgcgcggt
    62821 cacgtgcttc tttagttgct tgatatacag ctaacgcatc cataccatca acttggatac
    62881 caggaatacc tacagcaatt gctttttgag ctaatgtttc agcagcagtt tgcttgcttc
    62941 ttggtgttga aattgcatag ttattgtttt gaataacgaa aattgcaggt gctttataag
    63001 ctgctgcaaa gttaatacct tcgtagaaat caccttgtga agaaccaccg tcaccagtgt
    63061 aagtgattgc aactgcattt ttaccacgtt ttttaagtgc aaatgcaaca ccagcagctt
    63121 gaatgtattg tgcaccgata ataatttgtg ggcttaatgc attaacgcct tcagggaatt
    63181 gatttccttt gaagtgacct cttgagaata agaaagcttc agttaatggt aaaccatgcc
    63241 aaataatttg aggaacatct ctgtatcccg gtaaaatgta atcttctttt tctaaagcgt
    63301 attgtgacgc taattgtgat gcttcttgac cagcagttgg tgcatagaaa cctaaacgtc
    63361 cttgtctgtt taatgagata gaacgttgat caaggatacg agtccatacc attctttcca
    63421 ttaattccac taattgttca tccgtaagat caggtactaa gtcttcattt acgacgttac
    63481 cattctcatc caaaatttga accatttcaa atttcgattg agtatcattt aaaactttta
    63541 ctgcatcgaa ttgggcttgt aacttaggag ccattcaatt caccatacct ttcccataat
    63601 tgtatagaaa ttcattttat ccttactaat tgtaccacaa ataaatggga ctgttaaacc
    63661 atttgaaaaa gttctgtttt aataaaaaaa gatacagata ataggaactg tattagttgt
    63721 ttcctaccat ctgtattata ttaattattt aaattgatca acgtcttgct tttctttacc
    63781 aaccttattt agtacttttg tatacttatc tgatacttct tttaactttt tatagttctg
    63841 ttctattgct tttgattttt cgttaacacc ttgttgtgtc gcgtcatttt gatttaaata
    63901 tttaaataat gttttctctg agtttacagc ctttttatat gctttcgcgt aatcactgtg
    63961 taacttatat ttttctttta atacatcatc taattgtttt acttctttac gtttaacatc
    64021 attatcaatg ttatcaacgt gactttttgc tttcttaaag tcttgttcag acttcttaat
    64081 tgcgtcttct tctttttcaa attcctttag acgatcatcg gcatttttaa ttaaatcctt
    64141 tactgcttta ccgcgaactg ctgtatcttt actattgaca tcttcagtta attttttctt
    64201 tttctcatct aattcagcaa ttttcttacc aacagtttta attggttctt catcatcttt
    64261 aattttatcc acttgcttta aatatgcctt aatttctttt ttatccgtag tacatcctgc
    64321 aagcaagaca ctagatgcta atactactgc gattgttttt ccaaatttca ttacttgtac
    64381 ctccacttgc tatttaaatt aaatgataaa taaaatctgc ttggaaatca attttaaaca
    64441 attacattac acatcttcat tactaagtat atctgttgac agttacgtca tttataatat
    64501 aatttttaaa gtcaagatga taaataaatt tctaaagtat cattgcttga aaccaacact
    64561 ttaatcattt tttaagcaat tagcatatct gaaaatttac atatactatc taaaataaac
    64621 attggcagta gttggttaac tttgtagata tttacacata aaaatgtatg agtatcttaa
    64681 caaccttttt actgtcattc aactaaaaca aattaaaata aggaaggtgc aatatatgtt
    64741 aacaatgaaa gacatcatta gagatggtca tccaactttg cgtcaaaaag cagctgagtt
    64801 agaattacca ttaactaaag aagaaaaaga aacattaatc gccatgagag agtttttagt
    64861 aaatagtcaa gatgaggaaa tcgcgaaacg atatggttta cgttcaggcg ttggtttggc
    64921 tgcacctcaa attaatattt ctaaacgtat gattgctgtt ttaataccag atgatggcag
    64981 tggcaaatct tatgactata tgcttgtgaa cccaaaaatt gtaagtcata gcgttcaaga
    65041 agcttattta ccaactggtg aaggttgcct tagtgtcgat gataatgttg ctggtctagt
    65101 tcaccgtcat aatagaatta caattaaagc caaagacatc gaaggtaatg atatacaatt
    65161 acgactaaaa ggatatccag caattgtttt ccaacatgaa attgaccatt taaatggtgt
    65221 aatgttctat gatcacattg acaaagatca cccattacaa ccacatacag atgcagtaga
    65281 agtttaaaac acatttttct aattatcaaa gcttaggata aatatgatgt cctaagcttt
    65341 tccttttaca actttttcga ataaacaaca gttaaatata ttcacctttc taccaaactt
    65401 tttatcccct catttaaatt ttaccgtttc atataaaatc ctttaattct ttcttaacat
    65461 taatttataa tctctacatt tattttaata aatagaactg cacatttatt cgaaatactt
    65521 agatttctag tgagataaac tgctttattt attatcattc atcatgtaaa ataagattta
    65581 actgaaattt tagtgttatt tcactaattt tttaaaatga acgacatgat gaacctagtt
    65641 attaaccaaa tcgttattaa gttacattat agagatgatt ggaatgaatt tatcgatata
    65701 tactccaata cgattttact agggttaaca ataaattaaa caaacattct taggaggaat
    65761 ttttaacatg gcagtattta aagtttttta tcaacataac agagacgagg taattgtgcg
    65821 tgaaaataca caatcacttt atgttgaagc tcaaacagaa gaacaagtac gtcgttactt
    65881 gaaagatcgt aattttaata tcgaatttat cactaaatta gagggcgcac atttagatta
    65941 cgaaaaagaa aactcagaac actttaatgt ggagattgct aaataatgaa acaattacat
    66001 ccaaatgaag taggtgtata tgcacttgga ggtctaggtg aaatcggtaa aaatacttat
    66061 gcagttgagt ataaagacga aattgtcatt atcgatgcag gtatcaaatt ccctgatgat
    66121 aacttattag ggattgatta tgttatacct gactacacat atctagttca aaaccaagat
    66181 aaaattgttg gcctatttat aacacatggt cacgaagacc atataggcgg tgtgcccttc
    66241 ctattaaaac aacttaatat acctatttat ggtggtcctt tagcattagg tttaatccgt
    66301 aataaacttg aagaacatca tttattacgt actgctaaac taaatgaaat caatgaggac
    66361 agtgtgatta aatctaagca ctttacgatt tctttctact taactacaca tagtattcct
    66421 gaaacttatg gcgtcatcgt agatacacct gaaggaaaag tagttcatac cggtgacttt
    66481 aaatttgatt ttacacctgt aggcaaacca gcaaacattg ctaaaatggc tcaattaggc
    66541 gaagaaggcg ttctatgttt actttcagac tcaacaaatt cacttgtgcc tgattttact
    66601 ttaagcgaac gtgaagttgg tcaaaacgta gataagatct tccgtaattg taaaggtcgt
    66661 attatatttg ctaccttcgc ttctaatatt taccgagttc aacaagcagt tgaagctgct
    66721 atcaaaaata accgtaaaat tgttacgttc ggtcgttcga tggaaaacaa tattaaaata
    66781 ggtatggaac ttggttatat taaagcacca cctgaaacat ttattgaacc taataaaatt
    66841 aataccgtac cgaagcatga gttattgata ctatgtactg gttcacaagg tgaaccaatg
    66901 gcagcattat ctagaattgc taatggtact cataagcaaa ttaaaattat acctgaagat
    66961 accgttgtat ttagttcatc acctatccca ggtaatacaa aaagtattaa cagaactatt
    67021 aattccttgt ataaagctgg tgcagatgtt atccatagca agatttctaa catccatact
    67081 tcagggcatg gttctcaagg tgatcaacaa ttaatgcttc gattaatcaa gccgaaatat
    67141 ttcttaccta ttcatggtga ataccgtatg ttaaaagcac atggtgagac tggtgttgaa
    67201 tgcggcgttg aagaagataa tgtcttcatc tttgatattg gagatgtctt agctttaaca
    67261 cacgattcag cacgtaaagc tggtcgcatt ccatctggta atgtacttgt tgatggtagt
    67321 ggtatcggtg atatcggtaa tgttgtaata agagaccgta agctattatc tgaagaaggt
    67381 ttagttatcg ttgttgttag tattgatttt aatacaaata aattactttc tggtccagac
    67441 attatttctc gaggatttgt atatatgagg gaatcaggtc aattaattta tgatgcacaa
    67501 cgcaaaatca aaactgatgt tattagtaag ttaaatcaaa ataaagatat tcaatggcat
    67561 cagattaaat cttctatcat tgaaacatta caaccttatt tatttgaaaa aacagctaga
    67621 aaaccaatga ttttaccagt cattatgaag gtaaacgaac aaaaagaatc aaacaataaa
    67681 taatcaaaaa gctactaact ttgaagtgaa gttttaatta aactcaccac ccattgttag
    67741 tagctttttc tttatatatg atgagcttga gacataaatc aatgttcaat gctctacaaa
    67801 gttatattgg cagtagttga ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct
    67861 agtcaggggc cccaacatag agaatttcga aaagaaattc tacaggcaat gcgagttggg
    67921 gtgtgggccc caacaaagag aaattggatt cccaatttct acagacaatg taagttgggg
    67981 tgggacgacg aaataaattt tgagaaaata tcatttctgt cccactcccg attatctcgt
    68041 cgcaatattt ttttcaaagc gatttaaatc attatcatgt ccaatcatga ttaaaatatc
    68101 acctatttct aaattaatat ttggatttgg tgaaatgatg aactctttgc ctcgtttaat
    68161 tgcaataatg ttaattccat attgtgctct tatatctaaa tcaatgatag actgccccgc
    68221 catcttttca gttgctttca attctacaat agaatgctcg tctgccaact caagataatc
    68281 aagtacactt gcactcgcaa cattatgcgc aatacgtcta cccatatcac gctcagggtg
    68341 cacaaccgta tctgctccaa ttttatttaa aatctttgca tgataatcat tttgtgcttt
    68401 agcagttact ttttttacac ctaactcttt taaaattaaa gtcgtcaacg tacttgattg
    68461 aatattttca ccaattgcca caatgacatg atcaaagtta cggataccta aacttttcat
    68521 aactgcttca tctgtagtgt ctgcaacaac cgcatgagta gcgatatcac tatattcatt
    68581 cactctattt tcatcatggt cgatggccat tacatccatg tctaatgcat tcaactcacg
    68641 aacgatacta cctccaaaac gacctagacc gatgactaca tattctttac ccatactcgc
    68701 cctccattaa atgattttca tcaattcatt gaaaatataa atttaaaatt attataaatg
    68761 agtaccccaa ctaaattatc taaatgcagt aatgcaagta aatgaaagtt ggggtatcgt
    68821 ctcaacttat gatttctttc cttcaacata ttctttgtcg aaaacaaata atcttaataa
    68881 taatattaac gatggaagta ataaaagtaa acctaaaata aagacaatca ctaatgtcca
    68941 gcccatttct ggattaacat atgcatctgt aatttttaca aacggatata aaaggtatgg
    69001 caatttacta attccatagc caaagaacgc gaacatcatt tgtaaaataa caaatacaaa
    69061 agccaaacca tgttttttct taaagaatgt taacaatgaa gctaatgcaa agaataagaa
    69121 acttatacca aacatccacc aatagtcaaa aacagctgaa taaaaatgtt cagaattttg
    69181 aatgcgtaat gatagaaata cgaataaaca aatgataatc atcggcggcc ctaaaaatat
    69241 gtgccattgt cttgttaaat tatatgctgg ttcgtcattt gcttttttag cataatatgt
    69301 caaaaatcct gatgaaatat ataaaactga aataattgcc aagaatacta cagaccaagc
    69361 aaatgggctt aataataact gcacccaatc tagatcgata acattgtttc gaacattaat
    69421 atagccacct tctgtaatag ttaaagcagt agataatgaa gctggaatta ataatccact
    69481 taccccatac atgatcatcc atgctaattt tgtatcttga ccataatttt caaatgcata
    69541 aaaactgttt ctcaacgata tcataatcag tgctattgaa cctggtatta acaataccgt
    69601 gcctaaatat ttgattgact ctggaaagaa acctacgaat cctacgaaga agaaaacaaa
    69661 gaatacattc gtaacttccc aaactgggtt taaataacgt gaaattaagt gattaatttt
    69721 cttttcatca ccagttaact ttgaatgcaa tgcgaagaaa cctgccccaa aatctataga
    69781 agcaataatg atatagcaaa ataaaaacaa ccataacact gttataccta taaatgcata
    69841 aatcattttt ctatttctcc tccttgcttc ttggctaaac gatttacatc ttcatacgcc
    69901 ggtttatttt taaacatacg aattaatacg tatgcacatg tatacattaa aatgatgtac
    69961 aatatgccaa ataaaattgt aacgaaggtt attccgcctg cttgtgttgc tgcttctgcc
    70021 acgcgcatat aaccacgaac aatccaaggc tgtctaccca tctctgttaa gaaccatcca
    70081 aattctatag ctagcattga agctgggcct gttaataata ttccataaag catccattta
    70141 tgagtagaaa actttctaag ctttttaaac attaaagtta agacataaac acctgaaatg
    70201 acaaaacata aaattcccat cgttaccatt aaatcaaaga aataatggac gatcataggc
    70261 ggatgtaaac tttttggaaa atcatttaac ccttgtactt tagttttgac actattatct
    70321 gctaagaaac tcaatagtcc aggtaattca atcgcacctt taacttgctg agtcttttca
    70381 tctaacacac caaataataa taatttggca tgggaagatg tatcgaaatg ccattcataa
    70441 gctgctaatt tttcaggttg gaatttatgc aaaaattttg cagataaatc ccctgccaac
    70501 atagaaagta atgttgaaaa gaatccaact atcatagaca ttttcaaagc tttcttatgg
    70561 tagacagtat ctttaggttg acgattacgc aataatttaa aagctgctat tgatgcaata
    70621 acaaatgcca tcgtcatacc ggctgtagta attacgtgaa atgatcgaac tataaacgat
    70681 gggttaaaca tcgcttctat aggttgaaca ttgaccatct ttccattctt caactcaaaa
    70741 cctgcaggcg tattcataaa tgaattcact gaagtaatga agaatgctga gaaagagcca
    70801 ccaataatta ctggtatact aattaagaaa tgtgtccatt tatttttaaa acgatcccaa
    70861 gtatataaat atatacttaa gaaaatagct tcaaagaaga acgcaaatgt ttccataaat
    70921 aatggaagtg caataacgtg tccacccatt tccataaatg taggccaaat caatgataat
    70981 tgaagtccta taattgtacc tgtaacaact cccactgcta cagtaattgt ataagcttta
    71041 gcccatcttt tggccatagc tatatattga agatcatttt tgcgaatacc taaaaattct
    71101 gcaattgcga acattaaagg cataccaaca ccaatcgttg caaaaatgat atgaactgct
    71161 aaagtcatag ctgtcaaaaa ccgactgatt tcaactgtat ccatttaaaa acatcacctt
    71221 tttctttttt tgatgacaac acaatgaact taattataat tgctataatg tgtattttta
    71281 aaacattatg cgctatgaaa ttgtgtataa tttatgtcag ttcacaatgt gttcacaaat
    71341 ttgaatttca atgtataatt gtgtatatta cattagaata aagcacgaag gagcatgata
    71401 catgtcagaa ataatcgttt atacgcagaa tgattgtcca ccttgtacat ttgtaaaaaa
    71461 ttatctaaat gagcatcaca ttgattttga agagagaaat atcaacaatc aacaatatcg
    71521 aaacgaaatg atagattttg atgctttttc aactccgttt attttgttga atggcaatcc
    71581 aatgtaccat gttgatcttg atgaaatcaa caaagtatta aatatccaag attaattttc
    71641 atctaaatca acttcatcat ttatagctat cataaattaa atatcgattt ttaaattaac
    71701 taacattaat taaagaacta cattttgtta atgacacata gttgtttaat tagttagcaa
    71761 caacattcca ttgaaattaa cataaatcat aaaaaagaac ccgaagtatt cataattact
    71821 tacaatgaat acttcgggtt tatttataat tctgtctttc gaaagaatta attattttac
    71881 gtagttgtta actaattcaa taacttcttc ttgcgttgca cagtcgactg cacggttagc
    71941 aagttcagtc atttcatttt tacttaaacc attaatttgt cttcttgctt tcagaataga
    72001 cgttgcactc atagagaact catctaaacc taaaccaagc aataatggaa tagctgtttc
    72061 atctccagcc atttcaccac acatacctgt ccatttacct tctttatgtg acgcttcaat
    72121 aacttgttta actaaacgta agattgaagg gttatatggt tggtatagat atgatacacg
    72181 ctctgacata cggtcagcag ctaatgtgta ttgaattaaa tcatttgtac cgatactgaa
    72241 gaaatctact tctttagcaa agacatcagc taatgctgct gttgcaggta tctctaccat
    72301 gattcctaat tctatatcat ccgaaatgtc atgaccttca tttttaaggt tttctttttc
    72361 ttctaataat atagctttag cttctctaaa ttcgttaatt gttgcaacca ttgggaacat
    72421 gatatttaac ttaccataaa ctgatgcacg taataatgca cgtagctgtg gtctgaaaat
    72481 atcttgttgc gcaaggcata aacgaatcgc acggtaacct aagaatggat tcatttcttc
    72541 aggcaagttt aagtatgata attctttatc tccacctata tctaaagtac gtacaacaac
    72601 acgtttaccg cccattgctt ctaatacttc tttataagct tcaaattgtt cttcttctgt
    72661 aggcatttgg tcacgaccca tatataaaaa ctcagttcta tataagccga taccttgtgc
    72721 accattttca ataacacctg gcaaatcatt aggtgtacca atatttgcag caagctctgc
    72781 gtgaacacca tcaactgtaa cagtatcagc atcacgtagt ttttgtaatt ctttcttgtc
    72841 agcaaaataa cgctcacgtt tatcttgata agcgattaac tcatcttcag ttggattaac
    72901 gattacatca ccatttaatc catctacgat aatcatgtcg ccttgtttaa cttcttgagt
    72961 aattgatttt gtaccaacaa ttgctggaat ttctaaagaa cgactcataa ttgcagagtg
    73021 acttgttctt ccgccaatgt ttgtagcaaa accttgtacg aattctttat ttaattgagc
    73081 agtatcagat ggcgttaagt cattccctac aataacaacg ctttcatcaa tcatactcgg
    73141 attcggtaat tctacaccta aaatatgtga taacacacgt ttagaaacgt cgcgaatatc
    73201 agccgcacgt tctttcatgt attcgttatc catagattca aaaattgtaa caaattgtgt
    73261 tgttacatcc gttaatgctg tagcagcatt agcgttttca tttttaatct tatcttggat
    73321 tggttgaatt aattcagggt catctaaaac taataaatgt gcatcaaaga tagcagcttt
    73381 atcagcacct agttgaacct ctgcattatt tctaatttta gttaactcaa ctttagaagc
    73441 ttcgatagcg ctattgaact ttgcaacttc tccttcaaca tcagtgactt tttcattttt
    73501 gtcgaatgtt aagtctggct caactaataa ataagcttta gcaattgcga caccatcaga
    73561 tgcggcaata cctttaatta atttagacat gattatttag tcaatccttc ttttgataag
    73621 acgtcactga ttgcttgaat ggcgtcagat tcatcactac cgtcagcata aattgtaatt
    73681 tcagcatctt taccaacacc aaggctcata acacccatga ttgattttaa gtttactttc
    73741 ttaccgttat attctaattg aatatcagaa tcgaattttg aagctgtttg tactaacatt
    73801 gttgctggtc tagcgtgaat accagtctcg tcgatgatta catatgaatt ttgttccata
    73861 attacatttc tccttcgtat agttagaatc ttcaatatta ttacaatact aacttagttt
    73921 tatacattaa ctttaccaaa accatgcttt aaattcaatt cgtttaaaac cggtttcagt
    73981 aaaatttgca aattgcaaaa ctattatagc acctacatga catttttaat aacattttcg
    74041 catttttgaa tgtgatcttt tccaatttta ttcataaaat agtaactaat agatgcagac
    74101 acagcttgtc ctacaacagg aaaccatttt gtttgttttg cagcagtacg tttagctaca
    74161 tctctaatta caatttttaa aaatgcattt gaaattcttt taccaataaa ttgactacct
    74221 tgaattgctg ctgcagacat aattctttct ttcacatcat ccccaaggct attaacttgc
    74281 ttatgatcta aaccataaat tttattaaca tcttcgataa tatctttcat taattttaaa
    74341 tcaacaccaa aatctaaacc cggaattgga acaacgctca ttgttgatga cattaaagac
    74401 tttctcttta caattgcttc agcacgttgt cgtctttctc tcaattcttg aatcgtcgtt
    74461 ggcattgcac cttttccgtc aacattttct attttaaaga ctgaattacc gattttattt
    74521 gttaaatttg atgttaaatt gtttttgaat cccataatat tatcaccctt ctttttctca
    74581 ataagttact ataccccttt ttatcaaatt aatgtctaat atttacaaaa acagctttta
    74641 aatatttaga tggcttataa tgtggatgcg ttttaaaatc ttttggtaat cccataactt
    74701 cagttaattc ataatcaacg ccactctctt caagcgtctt tttaatagta tttttaaatt
    74761 gctttaatgg atatacactt gcgtttgtac ataacaataa tgttccttca gatgataaga
    74821 tatttaaggc gccattaatt aatttgtcat aatctttttg cactgaaaat gtacgttttt
    74881 tgttacgcgc aaagctaggt ggatcaatca cgatcgtgtc ataactatgt ccatgtcgtg
    74941 cagcatattt atagaaatca aaagtgtcca tgacataaat atattgggat ttaggatcaa
    75001 tagcatttaa tccaaaattt tcttcagtta aactacgaga acgattagcc aaatctacac
    75061 ttgttgttga agatgcctca cttgctgcta ttgcagaaaa agcacctgta taactaaata
    75121 agtttaaaac atggcgttct ttggcatatt gatcccttaa tttcttgcgc acttcttttt
    75181 gatctaaaaa gatacctgtc atcaaaccat cttcaaggtc tacattataa aatgtgaagt
    75241 tttcttcgat aacaattgga aactcaggtg catctccttc aacaaaacca ccactatatt
    75301 cgctgtcttt aaatcttact ttttcgtaaa tagatttata atcaaatact tttctaaccg
    75361 cttcaagaat ggcatattta aatttataaa taccttttga gtaccattga atcaacaaat
    75421 gaccatcgta attgtcgatt gttaatcccc caacaccatc accttcagca ttaaataatc
    75481 taaaagcatt tgttccatct atattaaaat aataatcacg ttctgctaat gcagtattaa
    75541 acaattttac aaagaaagct gtgttgattt gttgagcttt atcgtatgtt agaacccaac
    75601 ctaatccttt atgttggcga ccaacataag ccgttgcgac atattgtgat ttatcagtca
    75661 caatttgaaa aatatctcct tcttttaaat gatcttgtga atagatatcc tcttcttcaa
    75721 ttaaaggata tccattaaaa tattttgttt ctttgccttt gttcagagtt gctattttca
    75781 tctgacaact tcctttctgt gtttcgttgt agttttatat agattaaatt gtttgccaaa
    75841 tttcaatatc atctacttct aatcttactg ttgattttaa caaattagta attcgaatga
    75901 tatatgttaa atttctacaa aggaaatact atatatcgtt tcgcaaattc cataagtcgc
    75961 ttcaactatt tactagtttt cttgatttta caaatggata acttttcaac tattttattt
    76021 gcactaaaac tttgtataca attttcaata cttaattcac atcacctgaa tcaatcaaat
    76081 ctagttacaa aaaaagtact atccataaac ttcattgacc tgtatctaag taagttagag
    76141 atatatctaa cttatagcgt acacatcaaa atgtttaata gatagtactt ttataatgct
    76201 tttaattatg gtcgctctcg taattgtaac gaataagaaa tgatgattgc caataacatt
    76261 aatagtaatg ttggcgtaat gaacactgaa agtaatagtc catgaatcat tatatttata
    76321 aattccgtta acaatttaaa gaataaaatg ctccccataa ataattgtaa ataataaaac
    76381 ttacctttca agtggaaagt taacgttgta gcgatatatg cgataataat gaataacaga
    76441 attaataatg taacccattc aataacatat tgtgtctgtg ataatttcca atcaccagat
    76501 acaaataatg cattacgatt attaaattct aaaaatgtga ataaaaagat gacgacacca
    76561 caaaatagtt cgacatacat tggtttaacc catttcggtt ttctcatcca atcaggaata
    76621 tcgtcttctt gaatatcgat aatatcatta actttctttt tgaatttatc tcttttagct
    76681 ctaattttat ctaaatcgac acttcttctt gtcttacgtg catattctgt agtcttatct
    76741 tggagttgtt tcaagtgaat tttagtttga ttcgtgacat tccctttact tgttcttgat
    76801 aattctggct cctcttcacc taattcttgc tttgttaaag gtaaagctcg gcgcaagaac
    76861 aagaaattcc aaatagccat ttgaccaagt aaccacataa ttataaagaa agcattgaca
    76921 cttaagatat caattgactc tatttcatta gcatcaattc tgccatacaa tgcaatttgt
    76981 gtaattaagt aacttatacc ataactcaca attatccaca tgaaatgatt ttttacattt
    77041 tcctgattca attcatcttt atagacgata tatcctatat gaattgtaaa tggaataatg
    77101 aaaatcactg caaagaatcc ataaacttga gtcattaata taagagtaat aataaagtac
    77161 atgataccga taactaagaa aaagatagaa atatcatagt catacttaat agttctaagt
    77221 aatgtatgac caatcaacat aactaaaata ccgaatataa ttatcacaat tgcactaact
    77281 atagggaaat cacctataaa tcgactcatt actttaataa tttcactgaa aaatgcattc
    77341 agaaattccc acacattatg aacatgtgta tcaacttttt taccaccatt taattgttta
    77401 attaatggca agttaattaa aataatgaga ccagtgaaga gtgaaacgat accgattaaa
    77461 tagctaaaaa taatttcggc gtgtttccta agaaaagaca ttatgttcac ctcaaaatca
    77521 ttatatatta atctaatgtt gttgtcttta gataaatgaa taaatagtgc tatagactta
    77581 gaaaagttta acgaaagtac aaccgggtaa gatttatata taaataatta tccaatttgg
    77641 gaggaattaa tcatgtctaa tgaaaatcaa aataaaaaag cagcagaaaa agctaaagaa
    77701 gttgaagaaa aattaaaaga taaaaaagaa gaaaaaacag aagatattaa tcaaacaaaa
    77761 caagatatcc aagatacact aaattaattt aaatcccctt acaactttta gttgttaggg
    77821 gattttatgc atgtgctcgg cgcatgtatc cctctccttc cttacaactt ttagttgtta
    77881 ggggctttta tgcatgtgct cggcgcatgt atccctcttc ttccttataa actataattc
    77941 gtaagccatt atgcacgggt tcttcactgt tgttaaagag gtatgattga taagtatccc
    78001 tcttcattta tcagctcaat attattacat gtcacatatt tttatatctt taattactaa
    78061 cattcctact acatcttcaa tttaaatcca atgctaatat tataaatttt aaatcgaatt
    78121 aaacgaaatt tcatgaagac aacctattga cataagttta caaacgtctt acagtataat
    78181 taattaaata aatcgcacac actaggggtg ttttatactg agatgaggct tgccctcaaa
    78241 ccctttgaac ctgatctagc ttgaactagc gtaggaaagt gttactatac atatgtttta
    78301 ctaatatata ttgtgaacgc ataactttcc tatggatggt tgtgcgtttt tttattagga
    78361 ggatgtaaaa atgtcaaaag gtttaaagct atctgaaata ctagtaaccg tacttatttc
    78421 agtagtattc gcagttattt acaatttatg gtggtttgtt tacaatggtg tgcaagctac
    78481 aggccttcac ctcgaacaat taacaaacgg ggtttggttc atggcagcga ttgtctgcta
    78541 tctaattatt ccaaaaccag gtattgcttt actggctgaa tttgctgcag gtgccggcga
    78601 aactatcatt atgggtcgtt tcgatattcc gacaatcgtt tacgctttta tacaaggttt
    78661 agcgtgtgaa cttgtctttg caatttttaa atatcaatca cgttcagtta tggtagctat
    78721 gttagcaggt ttttgtacag caatcgcggc tttcccgata gattatttct atggttattt
    78781 aaatgaagtt gcaggttgga acttaacatt attcatcgta tttagattaa ttagtggtgc
    78841 agtagtcgcc ggcgtcctat cttacctttt agtaaaagct ttagataaga caggcgtaac
    78901 aaaattattt agaccagctg ctaaagaaga ttacgacaac ttataagagg agcattcaga
    78961 gtgttaaaag taagtgattt acgattaaaa tatccaagtg gtcaacgtaa aattttcgat
    79021 catttaaata tcactattca agacaaagaa aaagtacttt tactcggtcc ttctggttgc
    79081 ggtaaaagta cacttctgaa tgtattaagt ggtattgttc ctaatttaat tgaattacct
    79141 atgaaatatg atgaactaat cgttgaccca ttaagtggcg ttattttcca agaccctgat
    79201 agccagtttt gtatgccaaa agtatacgaa gaacttgcat tcgttttaga aaatagacaa
    79261 ttaccacgtg aagacatgga tgcgttaatt atcaatgctt taaatatggt caatttaaat
    79321 gttacccctg aaacgtatat caaagattta agtggcggga tgaaacagaa attggcaatt
    79381 gttgaaacca ttcttcaaca atcaaaaaca ttgtttttag atgaaccgac agcaatgtta
    79441 gatgttcaag caacagaaga tttatggact aaactaattg aactttggga agatcaaacg
    79501 gttgtaatcg ttgaacataa agttaaacac atctggaatc atgtcgaccg cgtcattttg
    79561 atggattata acggaaatat cattgccgat gaatgtcctg aaatcatatt acagaagtat
    79621 gttcatttac tcagtgaata tggtgtgtgg catccacgtg catgggaatt cgcaccaagt
    79681 cgtgttgact ttccaacaac aaactcacac ttattacaat ttaaaaatgg acgtattatt
    79741 cgcggtaaat caacattgct ctcattctca gatttagaaa ttggtctagg tgagtggatt
    79801 acaattacag gggcaaatgg tagtggtaaa acaaccttgc ttgaatcaat tatgcaattg
    79861 attaaatatc aaggtgatgt ttattttgaa aatcagcgtt taacaaaaat taaacatgca
    79921 gcaaaacaca tgtacctagt ttatcaaaac ccagaattac aatttataac aaattcggtt
    79981 tatgatgaaa ttaacattca ttttaatcac ctttctaaag atcaaagtga tgatgaaacg
    80041 atacaacttt taaaactttt agatttacaa aatgtaaaag atcaacatcc ttatgagttg
    80101 tctattggtc aaaaacgacg ccttagcgta gctaccgcac taagttctaa agctgatatt
    80161 atctttttag atgaaccgac atttggactt gatagccata atacattcca gttgatcaaa
    80221 cttttccaaa agcgaattaa tttgggacaa agtattgtca tggttacaca tgacgatgaa
    80281 ataattgaac gttatccatc aagaagactt aaaatttcag atggtgcgtt actagattgt
    80341 gatggtgata cgaatgtatg agcaatggaa agttcggcaa tcatttgttg atgatgtcaa
    80401 cattataacg aaactatttc ttggaattat actatttttc tttattatat ttgtgcataa
    80461 tttcgatttt atgctttata tcactatctt aatgttagtt ttcttattga tgtttaatgg
    80521 tactcagttt aaaattacag caatttttat aactataagt attggctttg cattactctc
    80581 ttccctgttc atgattttat acgggaatgg tagccataca attttgaaat tcggatttat
    80641 ccatattaca acagaaagtt tagttcgtgg tttacatgta tcattaagaa ccattgctat
    80701 ttcatttttc ggtatcttaa tcgcacttac atctcaggtt gtcatgatat tttatagttt
    80761 aatgcaacat ttaaaggtta aatcaaaagt tgcttatgca ttcatggctg ccattcgtat
    80821 gataccttta atgattagtt ctttaataca gcttcgcaga tctttaaaaa tgcgctatca
    80881 gatgatagac gcggcaaatt atagagggtt aaagcgattt aagcatttaa ttatacctct
    80941 attaagccaa aatattagaa aggcacatca attatcagtt gcgatggagt cgaaaggatt
    81001 tagagatggt cctcgtactt actattacaa cgtacctttc tcatataaag atataatctt
    81061 tattatagtt ttaattgcca ttattactat ggcatattat tgttcaatca ccttacccat
    81121 cacgggcata gatgatgttc gtcttggtcg tgtaggttaa attttatcca taattgtata
    81181 gatactaaat tagaaattca ttgtatagaa aaagaaaacc atttattcca tactaaacaa
    81241 aagaggtgcc acaaacatgt tattttgttc atccaaacat gttgtggcgc ctcttttgta
    81301 ttacatatgc ataatcacaa atttggtaca gaaatcaatg tcctagactc tacaactaaa
    81361 aaagctatga aaaaatctat cgttatagat tcgttcatag ctaatcttag tattttaaat
    81421 ttacttattt aagttgtagt gctttattcg caatgtcatg acgatagaat aaatggtcac
    81481 tttgtatttg tgatactttt ttgtatgcgt ctcgctgtgc atcttgtaca ttgtcacctt
    81541 ttccgatggc aagtataact ctaccacctg aagtaacaaa ggtatcacct tgcttcttta
    81601 atccactaac aaaatagttt tcatttaaat caaagccact tactttatgc cctttttcat
    81661 atgcatcagg atatcctttt gatgccaaca tgacccctac aatagattca tttttccatt
    81721 tgaattcagt acgttttcct tcatctaaat caataatatg ctgcattaaa tcactttcca
    81781 tgcgacttaa taatacttga gcttcaggat caccaaaacg ggcattaaat tctattactt
    81841 ttggaccatc tttagttaaa atagcaccaa tgtataatac accgaagaat tgataacctt
    81901 cattaagcat tgcctttgca atgggttgtg caattgtttc atttgtaagt tttaaaacat
    81961 cgtcactaat atgtggtact ggacaataag cccccatacc accagtattt ggtccttcat
    82021 catgatcaaa tgcgcgttta tgatcttgtg caatacagtc gaaaggtact gctaaatcac
    82081 cattaacaaa tgtcattagc gagaactctt caccttctaa aaacgtttca aatacaacag
    82141 taccttcttc ttcatcacca tacataatct caatagcact tctggctgct tcaatagtat
    82201 ctgcaataat aacgcctttc ccagcagcta acccatcttt cttgacaaca acgggcaatt
    82261 cacagttttc aatatatgtt aaagcatcct tttttcgctc aacttcttta taatcagcag
    82321 ttggaatatt atatttttcc attatctttt tagcaaataa ttttgagcct tcgatttgag
    82381 ctgcttgctt atttggacca aacactttga aaccattcgc tcgtaaaatg tctgctaatc
    82441 catcaattag cggctgttct ggacctataa ctacccaatc aacattttgc cgtttagcaa
    82501 aatctagtat cgcttgatga tcagattctg aaatttcagt gtgtacttca gctataggtg
    82561 tcattgcctc attacctgga atgacaaaca cttgtttaac tagattcgat tgattaagtt
    82621 tatatgcaag tgcatgttct cgtccaccag caccaattac taatacattc atttatgtta
    82681 cgccccttta aaattagtgt ttaaaatgtc gagtgcctgt gactaccatt gcaataccat
    82741 gtttattagc catatcaatt gaatcttgat ctttaatcga accactcggt tggataattg
    82801 cctttatacc atgttgtgct gcaagttcaa ctgtatctcc catagggaaa aatccatcag
    82861 atactaacgc tacatgatca ttgatttcaa tagctctctc taacgcaatt ttagcagcac
    82921 cgacacgatt catttgtcca gcacctatac ctacagtttg tttattatta cttaaaataa
    82981 ttgcattact ttttactgat ggtacaactt tccatcctaa taacattgct tcccattgtt
    83041 catctgtagg tgctacttct gtaacaactt tcatttcttc ttttggcacg acataattgt
    83101 ctttatcttg aactaaatat ccgccagata ctgaaacaaa ctcttcttcg ttactgtcta
    83161 tagtcatatc aatttctaat aatcttacat tcttcttttg ttttaaaata tctaatgctt
    83221 catctgtaaa ttttggtgca ataatgactt ccaaaaagat actatgcaat tgctctgcta
    83281 actcaggtgt tacagctcgg tttaatgcaa caattccacc aaatattgat tgactatccg
    83341 cttcatacgc atgttgaaat gcttgttcta tcgtgtcacc gataccaaca ccacatggat
    83401 tcatgtgttt aaccgcaact gtagcaggtg tatcaaactt tttaactaaa gctagtgtag
    83461 catctgcatc tttaatattg ttatagctta attgtttccc atgtaattgt ttagcgcctg
    83521 caatcgtgtg cttagcattc gaagttctca caaaatacgc tgattgttgt ggattttctc
    83581 catatcttaa agtttcttta tcccctttaa agaaacgtac aatcgcttca tcatattctg
    83641 cagtatgctc aaaaacttta atcattaatg attgtctata tgactcatct aacgaatcgt
    83701 ttcttaatcg cgtcaatact tcttgataat ctgccggatg tacaattgtt gttacatgtt
    83761 tatagttttt agctgcagca cgtaacattg ttggaccacc aatatcaata ttttcaattg
    83821 cttcgtccat cgtcacatca gggtttgcaa cagtttgttg gaatggatat aaattaacta
    83881 ctaccatatc aattaaatct atatgttgtt ctgataattc atttaaatgc tgcggtttat
    83941 ttcgatcagc taaaatgcca ccatgaacag ccggatgtaa tgttttaaca cggccatcca
    84001 ttatttctgg aaaatgtgtt aagtctgaaa cagaacgaac tggtacattt gcttcatcta
    84061 atatacgttt agtaccacct gttgaatata attcataatt taattgcgtt aacgctttag
    84121 caaactctac aatacctgtt ttatttgata cgctcaaaat agctttcttc atcacataca
    84181 tactccttat tttacaattt tagcaataac acttggatat aactcatatt ccaatttttt
    84241 tactttctct tctaattgtt cttttgaatc gtccggtcta atatcacatt ttctctgttc
    84301 aataatttct cccgtatcca taccactatc aacataatgt actgtcgaac cagtaatagt
    84361 atcgccacta tgatatgctt ggcctattgc gtcaatcccc ttatatttcg gcaatagaga
    84421 tggatgtata ttcaatattt taccttcaaa tgaagctaat aagtctggac caattagacg
    84481 catgtagcca gctagaataa tccactctac cttatcttca tttaataatg ttactaaatg
    84541 ttgttcatac gctgcttttg aatcaaattg ttttggttca ttaatataaa caggaatatc
    84601 gtgctttttt gctctatcta tacaaaacgc attttgatga tccgtatata gcgccgtaac
    84661 ttcaatattt tcaagttttc ctgattcaac atgctcaact atattttcaa agttacttcc
    84721 tgaacctgat gcaaaaatcg caattttaac cattgttata cccccaacaa ttcaattgca
    84781 gttgactcat ttttcacaat atgaccaatt tgataggctt ccacattttg ttctgctaaa
    84841 atcttcaaag cgcgtgatac atctttttca tcaacgataa ccgtatagcc aatacccata
    84901 ttaaaaatgt tatacatttc atttgtgtct atattgcctt gttgttgtaa ccaatcaaat
    84961 atttttggtg ttggaaatga tgtagtatca attctagcag catatccggc tggcaatgca
    85021 cgtggaatat tttcataaaa acctccacca gtaatatgat tcattgcctt aatagaaact
    85081 tcttttttca aagcaagtac aggtttgaca tataatttag ttggttctaa aaagacatcg
    85141 ataaatggac gattatcgaa gtttgatgcc aaatcaatgc ctgattcatt aattaatttg
    85201 cgcactaaac tgtatccatt tgaatgaatg ccacttgacg caagccctat aacaacttgt
    85261 ccctctttca cttctgaacc atctacatag tcatcctttt caactgctcc aacagcaaat
    85321 ccagctacat catattcgcc ttcgtgatac atttcaccca tttcagcagt ctctccaccg
    85381 ataagtgcag tattcgtttc aacacatgca tcactaatac ctttaacaat ttgttcaata
    85441 acttcaggaa caactttgtt tgtagcaatg taatctaaaa aatataatgg ttctgcacct
    85501 gtcgttaaaa tatcattaac acacattgcg actgcatcga tacctatcga atcatgttta
    85561 ccatagtcga tagctagttt taatttcgta cctactccgt ctgttccaga aactaaaact
    85621 ggcgctgtca tatttaattg tgataaatca aatgtagcac cgaatccacc taaaccaccg
    85681 ataacttctt tacgcatcgt acgtttaaca tgactagaca ttctttctac agcttcataa
    85741 ccagcatgaa tatttacacc agattgttca tatgctttag acatttaaat ttccctcgct
    85801 atcaaaaaag tgtttgtttt tagaaatata ttgcttttgt cgatgactta aatgcgcttt
    85861 ataatttgct tcataatcat ataaccctgc aggataatct ccagtgaaac tttctacaca
    85921 taagccacta tatggcgcgt cataatctaa accaattgat tcaattaacc catctacaga
    85981 tagatatgct aatgaatcag cgccaatata atctttaatt tcttcaggtg atttgcttgc
    86041 agaaattaat tctgccgtag ttgaaacatc gattccgtaa aaacttggaa acataaattc
    86101 cggtgatgct atacgcacat gcactttatt tgcaccagaa tcttttaaca ttttcacaat
    86161 gcgtcgaatt gtcgtaccgc gaacaatgga atcatcaaca agaatgatat ttttcccatc
    86221 tactatatct tttaccgcag ataacttcac tctcacacct tgctcacgta attcttgagt
    86281 tggttgaata aatgttcttg caacatattg atttttaact agtcccattt catatggcaa
    86341 acctatttct tcagcataac cactcgcagc tgatagcgat gaattgggta caccgatgac
    86401 catatcagca tttacagggc tttcttgggc taatttttta ccagaagctt tacgtactgc
    86461 atggacattt ttaccagcta ttgttgagtc tggtctagca aaataaatat attccatcgc
    86521 agaaattgca gttgtcgtat gatgtgtata agatttaact gtaatacctt tatcgttaat
    86581 cacgacatat tcacctgcat gaatatcttg aacaaattct gcacctaaca catctattgc
    86641 acatgtttca cttgcaagga tgtatgtccc atctttcatt ttacctacaa caagtggtct
    86701 gatagcattt ggatctactg cgccatataa cgcatcttta gttaaaatcg caaatgtaaa
    86761 accgccttta acttttcgca aactttcttt caacgcttcc tcaaaagtag gagctttact
    86821 tcgacgtatc aaatgcataa tgacttcagt atcagaagac gaatggaaga tagcaccttg
    86881 tttttctaaa ttctgacgca atgatttagc attaatgagg ttaccattat gacaaatacc
    86941 tacactcata tcataaaagt gatacagaaa cggttgaata ttttcaatac ctttattacc
    87001 tgaagtagca taacggacgt gaccaattgc atgttgatat ccttttaatc gttccatttg
    87061 atcatcttta atcgcttcag ttagtaagcc taatcctcgc tcgcctttta attcattttg
    87121 atcagaaaca actatacctg caccttcttg accacgatgt tgcaaactat gaagtcccat
    87181 atatgttagt tgcgctgctt caggatgatt ccaaatacca aacacgccac attcttcgtt
    87241 taatcctgag tagttaaaca ttgagcaatt gccccttccc atatttgttt aatatctgaa
    87301 acattttcac taatctctgt atatggtgtt gttaccttga aattatcact atctgttaaa
    87361 agtccaattt ctattgcatt atcaatattt aaagttttac ctgatttaac agaaacaaca
    87421 tatcggcctt gcgtctcact aaacaattgt gcatttgtta tatctattga agattttaat
    87481 cctaaaccgt aatgcgcact tagtttagct aaggtaatca gtaagccacc tttaccaact
    87541 gtttgaacat gtgataatag tccttcacga atagcggtct tgattgattc acctttttca
    87601 acttctgaac tcaaatctaa tgactcaaat tcatgattaa ctttgccata aattaacttt
    87661 tcaagttgac taccaccaaa gtcgtcctta gtatcaccga ttaaatataa tttatctcca
    87721 acttgaggtt caaaatcatt taaataattt acattttcaa tcaaacctac cattccaaca
    87781 actggtgttg ggaaaataga agtacctttc gtttcgttat ataaagatac attaccagaa
    87841 actactggtg tcttaagaat gtcgcatgct tctgccatac ctttcgttga atctatcaac
    87901 tgttgataga tttctttctt ttcaggagaa ccataattta aacaatctgt cattgctaat
    87961 ggtgttgcac ccacggcaat taaatttcga taagcttcag ctactaccat ctttccacct
    88021 tcatatggat tgttatatac ataacgcgct tcaccatcaa ttgttgaagc aattgcctta
    88081 tttgtgcctt ccacacgtac taccgatgct tgaagtcctg gcttaattat cgtattggca
    88141 ccaacttgtt ggtcgtattg atcatataaa tagtgtttag atgctatagt cggatgctta
    88201 agtaatttaa agaaagtatc tttaacatcg atgtgtgtat aatcattttt agaagtatta
    88261 taatcttttt cttctccttc taaaatatat acaggtgctt catcagctag tggttcaact
    88321 ggaatgtcag cataaacttc gtcatcatat gttaaaacaa aacgatttgt atctgtaact
    88381 tcacctataa cagcactatc caattcgtgc ttatcaaata aatctaagaa tttttgttca
    88441 gtaccttttt caacaactag taacatacgt tcttgagttt ctgaaagcat catttcataa
    88501 ggagaaatac ctggctcacg tgttggcact tgttctaatc tcaaatgtaa cccactacca
    88561 ccttttgccg ccatttcaga cgatgaagat gttaaaccag cagcacccat atcttgaata
    88621 ccaactaatt catcaaatgt aattgcttca agtgttgctt ccattaattt tttacctaca
    88681 aatggatcac cgatttgtac agaaggtcgt ttactttcgc tttcttccgt caattcttca
    88741 gatgcaaaag tagcaccatg aataccatct cgaccagttt tcaaaccaac ataaatgacc
    88801 gaattaccta caccttttgc tgtgcctttt tgaatcatgt cgtgattgat aacaccaaca
    88861 cacattgcat taacaagtgg attgccatca taacgttcat caaattcgat ttcaccagca
    88921 gttgttggaa taccaatgca gttaccataa cctccgatac cctttacaac acctttaagt
    88981 aatctttggt tttgtttatt atctaattct ccaaatctaa gactgtttaa caaattaata
    89041 ggtctagccc caatagagac aatgtcacga atgattccac caacgcctgt agcagcccct
    89101 tgatatggtt caattgctga tggatgattg tgagactcta ctttaaatac tacggcttga
    89161 ttatcaccta tatcgactac ccctgcacct tcaccaggcc ccataagcac atggtcacct
    89221 gacgtaggaa attgctttaa aaacggttta gaatgtttat aagagcaatg ttcactccac
    89281 ataacagaaa agatacctgt ttctgtaaag ttaggttgtc tgcctaaaat atcgcaaact
    89341 ttttcatatt cttgatcact taatcccata tcttgatata ctttttcaag tttaatttct
    89401 tcaacgcttg gttcgataaa tttagacatg ttgttccctc caacttttta ccatcgcttc
    89461 aaataatttc acaccactat cagtacctaa caacgtttct aaagctcttt caggatgtgg
    89521 catcatgcca catacattgc ctttttcgtt aacaattcct gcaatatcat catatgaacc
    89581 gttcggatta ttcacatatt tcagaataat ttgattgtta gcttttaatt gttgatatat
    89641 ttcatcagta caataataat gaccttcacc gtgagctaca ggatatataa ctttttcacc
    89701 ttgttcataa agatttgtaa atgccgtttg attattcact atttctaact cttcatttct
    89761 actaataaat aaatgtgaat cgttatgcaa taatgcacca ggtaataagc ctatttcagt
    89821 taaaatttga aacccattac aaacacctaa tactggctta ccttcagctg caagacgttt
    89881 aacttccgaa ataatcggcg ctacactagc cattgcccca gatcttaagt aatccccgaa
    89941 tgaaaatcca ccaggaataa gtacgccatc aaatccactt agtgatgttt ctctataatc
    90001 tacatattcc gcttcaacac cacttttaat agcagcatta aacatgtctc tatcacaatt
    90061 cgaacctgga aaaacaagaa ccgcaaattt cattttatgc attctccttt tcatcatcta
    90121 acactttata gctatattct tcaatcactg tatttgcaaa caatttttca cttagagttg
    90181 taataatgtt gtgtaccttt tcatcactaa cctcatccac tgtcatatat aatacttttc
    90241 ctacacgaat atcattcact tgtgcataac ctaagtcatg tacagctcga gtaagcgttt
    90301 gtccttgcgt atctaatact tgtggttgta atgtgatatg tagttcaatt gttttcatta
    90361 ttttaaatcc tccaatttgt ttaaaaatat ttgatatgtt tcaatcagtg atccagtgtt
    90421 atttctatat acatctttat caaagtttgc attggtagct ttatcccaaa ttcgacatgt
    90481 atctggagat atttcatccg ctaacaaaat ttgaccagtc tcagtctttc caaattcgat
    90541 tttaaaatct actaatttta aattcatagc atccattaat tgtttcaaca cattattaat
    90601 ctttaatgct ttggatttta gtatttcaat atcttcatct gatgctatat tgagcaattt
    90661 aacatggtca tccgttatca acggatcatt taacgcatca tttttataga aaaattctac
    90721 aagtggttct ctaaaaactt caccattttc aaaacctaaa cgctttgtaa tagatccact
    90781 agcaatatta cgaacaacta cttctaatgg aattattttc acaggcttaa ctaattgttc
    90841 tgtttcagat aattgtttaa taaagtgact ttctattcca ttttcttgta aatatttaaa
    90901 tataatagaa gtaatttgat tatttaatcg ccccttacct gccattgtgt ctttcttagc
    90961 cccgtttcca gcagtaactt catctttata ttcaactctt aattcatttt cttgatttgt
    91021 tgagaaaatg cgcttcgctt ttccttcata taataatgtc atgctttaat tactcccctc
    91081 aaatttagcg tacatatctt gttcagtttg gtttacatca ttcgttagta cagtcatatg
    91141 ccccattttt ctgctatctt tacgctcaga cttaccataa atatgtaagt gccactctgg
    91201 atgttcatta aattcatttt ccaataaatc taaatcttta cctagtaagt tcatcatgac
    91261 tgctggcttt aataattcaa ttgaatttgg taatgattgt ccggtaactg ctaaaatatg
    91321 agtatcaaat tgtgaataat cacatgcttc aattgaataa tgtccggaat tgtgaggcct
    91381 tggtgctatc tcgttcacat acaattggtt gttactatct ataaaaaatt caactgtaaa
    91441 tgttccaatg aaatgaatcg attggataat tttattaact tgctctttcg cctcagctgt
    91501 tttatctatt ctcgctggaa caattgtttt gaaaagtatt tgatttctat gctcattttc
    91561 ttgtaatggg aaaaaagtga tttgattgtt gtttcctctt gtaacagtaa gagatacttc
    91621 tttcttgata ttcaaatatt tttcagctac gcattcacta gtttcaatta atttaaaacc
    91681 ttcttgtaag tctttttcgt tgttaattaa aacttgacct ttgccatcgt agccaccaaa
    91741 tctagttttt acaataaaag gatatcctaa tgtttcaatt gctttgtcaa tatctgtaga
    91801 ttcttttact gaaatgaacg ggacaacttt ggtaccagca ctttttaatg tttctttttc
    91861 agttaagcga tcttgtaata actgtatagc ttggtaacct tgcggaatat tgtacttttc
    91921 acataatagt tttaattgtt gggctgaaat gttttcaaat tcataagtaa tcacatcaca
    91981 tttttgtcct aattgattga gtgccttttc atcgtcatac ttggcttgta taaattcgtg
    92041 tgcaacgtat ctacatggac aatcttcaga aggatccaat acaaccactt tataacccat
    92101 tttttgagct gattgtgcca tcatctttcc aagctgacca ccaccaataa tgccaatagt
    92161 cgcaccaaac tttaatttat tgaagttcat tttgcatgtc ctccactttt tgaattaacg
    92221 aagattcata ctgatttagt ttttcaacta aagaaggatt ttgaatactt aacattcttg
    92281 ctgcaagtat acctgcgttt ttagcacctg ctgcaccaat tgcagtcgtt gcaaccggaa
    92341 tacctcctgg catttgaaca attgataata aagaatctat accctttaaa ctttttgttt
    92401 caatcggcac tccaataact ggtagcgtcg ttaatgatgc aaccatacct ggtaaatgtg
    92461 ccgcaccgcc agcgcctgca atgataatgt ttatacctct ttctctcgct tcagaagcaa
    92521 attgaaccat catttttggc gtacgatgtg cggatactac ttgtttttcg tacggaattt
    92581 caaaataatc caacatgtta caactctctt gcataatttt ccaatcggaa gaactgccca
    92641 taatgactgc tactttcact ttgtacaccc tttcaaaagt ttgaattgtg aattacttta
    92701 gttgtatatt atagatatag cataacaagc aatttctgct ttttcaatca aaaatcgaac
    92761 tttattttga ttttttgttt gaatttacgt cttttgctat gtaaattagt tttataaact
    92821 aacaaagtta ggatattgac aataggagga gaagttttta tggttgctaa aattttagat
    92881 ggtaaacaaa ttgccaaaga ctacagacag gggttacaag atcaagttga agcgctaaaa
    92941 gaaaagggtt ttacacctaa attatccgtt atattagttg gtaatgatgg cgctagtcaa
    93001 agttatgtta gatcaaaaaa gaaagcagct gaaaaaattg gtatgatttc agaaatcgta
    93061 catttggaag aaacagctac tgaagaagaa gtattaaacg aactaaatag actaaataat
    93121 gatgattctg taagtggtat tttggtacaa gtaccattac caaaacaagt tagcgaacag
    93181 aaaatattag aagcaatcaa tcctgaaaaa gatgtggacg gttttcatcc aataaatata
    93241 gggaaattat atatcgatga acaaactttt gtaccttgca caccgctcgg catcatggaa
    93301 atattaaaac atgctgatat tgatttagaa ggtaaaaatg cagttgtaat tggacgaagt
    93361 catattgtcg gacaaccagt ttctaagtta ctacttcaaa aaaatgcatc agtaacaatc
    93421 ttacattctc gttcaaaaga tatggcatca tatttaaaag atgctgatgt cattgtcagt
    93481 gcagttggta agcctggttt agtaacaaaa gatgtggtca aagaaggagc agtaattatc
    93541 gatgttggca atacgccaga tgaaaatggc aaattaaaag gtgacgttga ttatgatgcg
    93601 gttaaagaaa ttgctggagc tattacacca gttcctggtg gcgttggtcc attaacaatt
    93661 actatggtat taaataatac tttgcttgca gaaaaaatgc gtcgaggtat tgattcgtaa
    93721 agagcctgag acataaatca atgttctatg ctctacaaag ttataatggc agtagttgac
    93781 tgaacgaaaa ttcgcttgta acaagctttt ttcaattcta gtcaaccttg ccggggtggg
    93841 acgacgaaat aaattttacg aaaatatcat ttctgtccca ctccctaata actgagtttt
    93901 aataaagtct tttaacccac attaaatatt attttgcaat tgcaatgaat aacaagaaaa
    93961 atctgggaca ttaatcgatc aaatgctccc ttcaaagtag acattgaata aatgaaggct
    94021 ttgaagggag catttcactt tgtacttggc tcaacaattt tatatagaca gtagttaatt
    94081 gaatgaaaat aagcttgtaa caagttttca attctagtca gggccccaac aaagaagctg
    94141 acgaaaagtc agcttacaat aatgtgcaag ttggggatgg gccccaacac agaagctgtg
    94201 actatgataa agtactacta catagttaat cattagtggt tctttatcat tttcgcctcc
    94261 cttttcttat tgttttgata cacaaaaatt taagttcaaa ctgtcgaata aagttatatt
    94321 tgatttcaaa ttatccctaa attattaatt ttacaattgt ggcagatttt caaaataata
    94381 attatttcct cattatttat aaatttatat ttaaatttca ttctttatag ggtaagatta
    94441 ggactatagt atgatgtgta aataatataa attaa
//
LOCUS       DANJFZ010000002        60684 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.002, whole genome shotgun sequence.
ACCESSION   DANJFZ010000002 DANJFZ010000000
VERSION     DANJFZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 60684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.002"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            50..721
                     /locus_tag="PWN45_000096"
     CDS             50..721
                     /locus_tag="PWN45_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDL0621900.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(851..1654)
                     /gene="coaW"
                     /locus_tag="PWN45_000097"
     CDS             complement(851..1654)
                     /gene="coaW"
                     /locus_tag="PWN45_000097"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDL0621901.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            2078..2938
                     /locus_tag="PWN45_000098"
     CDS             2078..2938
                     /locus_tag="PWN45_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0621902.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            3050..3580
                     /locus_tag="PWN45_000099"
     CDS             3050..3580
                     /locus_tag="PWN45_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL0621903.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            3916..5526
                     /locus_tag="PWN45_000100"
     CDS             3916..5526
                     /locus_tag="PWN45_000100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL0621904.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(5635..6156)
                     /locus_tag="PWN45_000101"
     CDS             complement(5635..6156)
                     /locus_tag="PWN45_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDL0621905.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            6374..7234
                     /locus_tag="PWN45_000102"
     CDS             6374..7234
                     /locus_tag="PWN45_000102"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL0621906.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            7700..8959
                     /locus_tag="PWN45_000103"
     CDS             7700..8959
                     /locus_tag="PWN45_000103"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0621907.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            9048..9383
                     /locus_tag="PWN45_000104"
     CDS             9048..9383
                     /locus_tag="PWN45_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0621908.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            9621..11048
                     /locus_tag="PWN45_000105"
     CDS             9621..11048
                     /locus_tag="PWN45_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDL0621909.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            11295..12611
                     /gene="rho"
                     /locus_tag="PWN45_000106"
     CDS             11295..12611
                     /gene="rho"
                     /locus_tag="PWN45_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL0621910.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            12729..12983
                     /locus_tag="PWN45_000107"
     CDS             12729..12983
                     /locus_tag="PWN45_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL0621911.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            13330..13929
                     /locus_tag="PWN45_000108"
     CDS             13330..13929
                     /locus_tag="PWN45_000108"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0621912.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            13930..15006
                     /gene="prfA"
                     /locus_tag="PWN45_000109"
     CDS             13930..15006
                     /gene="prfA"
                     /locus_tag="PWN45_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL0621913.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            14993..15829
                     /gene="prmC"
                     /locus_tag="PWN45_000110"
     CDS             14993..15829
                     /gene="prmC"
                     /locus_tag="PWN45_000110"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL0621914.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            15913..16959
                     /locus_tag="PWN45_000111"
     CDS             15913..16959
                     /locus_tag="PWN45_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL0621915.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            16956..17375
                     /locus_tag="PWN45_000112"
     CDS             16956..17375
                     /locus_tag="PWN45_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDL0621916.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            17482..18006
                     /locus_tag="PWN45_000113"
     CDS             17482..18006
                     /locus_tag="PWN45_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL0621917.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            18033..19271
                     /locus_tag="PWN45_000114"
     CDS             18033..19271
                     /locus_tag="PWN45_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL0621918.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            19299..19928
                     /gene="upp"
                     /locus_tag="PWN45_000115"
     CDS             19299..19928
                     /gene="upp"
                     /locus_tag="PWN45_000115"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL0621919.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            19952..21079
                     /gene="wecB"
                     /locus_tag="PWN45_000116"
     CDS             19952..21079
                     /gene="wecB"
                     /locus_tag="PWN45_000116"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL0621920.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            21147..21599
                     /locus_tag="PWN45_000117"
     CDS             21147..21599
                     /locus_tag="PWN45_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL0621921.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            21620..22348
                     /gene="atpB"
                     /locus_tag="PWN45_000118"
     CDS             21620..22348
                     /gene="atpB"
                     /locus_tag="PWN45_000118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL0621922.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            22391..22603
                     /gene="atpE"
                     /locus_tag="PWN45_000119"
     CDS             22391..22603
                     /gene="atpE"
                     /locus_tag="PWN45_000119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL0621923.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            22801..23322
                     /locus_tag="PWN45_000120"
     CDS             22801..23322
                     /locus_tag="PWN45_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL0621924.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            23322..23861
                     /locus_tag="PWN45_000121"
     CDS             23322..23861
                     /locus_tag="PWN45_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL0621925.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            23883..25391
                     /locus_tag="PWN45_000122"
     CDS             23883..25391
                     /locus_tag="PWN45_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL0621926.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            25422..26288
                     /locus_tag="PWN45_000123"
     CDS             25422..26288
                     /locus_tag="PWN45_000123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL0621927.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            26310..27722
                     /gene="atpD"
                     /locus_tag="PWN45_000124"
     CDS             26310..27722
                     /gene="atpD"
                     /locus_tag="PWN45_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL0621928.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            27742..28146
                     /locus_tag="PWN45_000125"
     CDS             27742..28146
                     /locus_tag="PWN45_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL0621929.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            28484..28576
                     /locus_tag="PWN45_000126"
     CDS             28484..28576
                     /locus_tag="PWN45_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621930.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            28708..28941
                     /locus_tag="PWN45_000127"
     CDS             28708..28941
                     /locus_tag="PWN45_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL0621931.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            29052..30317
                     /gene="murA"
                     /locus_tag="PWN45_000128"
     CDS             29052..30317
                     /gene="murA"
                     /locus_tag="PWN45_000128"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0621932.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            30351..30791
                     /gene="fabZ"
                     /locus_tag="PWN45_000129"
     CDS             30351..30791
                     /gene="fabZ"
                     /locus_tag="PWN45_000129"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0621933.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(30850..31290)
                     /locus_tag="PWN45_000130"
     CDS             complement(30850..31290)
                     /locus_tag="PWN45_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDL0621934.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            31484..31831
                     /locus_tag="PWN45_000131"
     CDS             31484..31831
                     /locus_tag="PWN45_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0621935.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            32268..32963
                     /gene="sceD"
                     /locus_tag="PWN45_000132"
     CDS             32268..32963
                     /gene="sceD"
                     /locus_tag="PWN45_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDL0621936.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      33167..33269
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33349..34038
                     /gene="tenA"
                     /locus_tag="PWN45_000133"
     CDS             33349..34038
                     /gene="tenA"
                     /locus_tag="PWN45_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDL0621937.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            34031..34861
                     /gene="thiD"
                     /locus_tag="PWN45_000134"
     CDS             34031..34861
                     /gene="thiD"
                     /locus_tag="PWN45_000134"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL0621938.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            34845..35636
                     /gene="thiM"
                     /locus_tag="PWN45_000135"
     CDS             34845..35636
                     /gene="thiM"
                     /locus_tag="PWN45_000135"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL0621939.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            35638..36279
                     /locus_tag="PWN45_000136"
     CDS             35638..36279
                     /locus_tag="PWN45_000136"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL0621940.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            36365..37237
                     /gene="yidC"
                     /locus_tag="PWN45_000137"
     CDS             36365..37237
                     /gene="yidC"
                     /locus_tag="PWN45_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0621941.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(38050..38697)
                     /locus_tag="PWN45_000138"
     CDS             complement(38050..38697)
                     /locus_tag="PWN45_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0621942.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(38725..40209)
                     /gene="cls"
                     /locus_tag="PWN45_000139"
     CDS             complement(38725..40209)
                     /gene="cls"
                     /locus_tag="PWN45_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0621943.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            40375..40668
                     /gene="csoR"
                     /locus_tag="PWN45_000140"
     CDS             40375..40668
                     /gene="csoR"
                     /locus_tag="PWN45_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDL0621944.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            40680..40889
                     /locus_tag="PWN45_000141"
     CDS             40680..40889
                     /locus_tag="PWN45_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL0621945.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            41017..41154
                     /locus_tag="PWN45_000142"
     CDS             41017..41154
                     /locus_tag="PWN45_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621946.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(41418..42620)
                     /locus_tag="PWN45_000143"
     CDS             complement(41418..42620)
                     /locus_tag="PWN45_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL0621947.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            42938..44008
                     /locus_tag="PWN45_000144"
     CDS             42938..44008
                     /locus_tag="PWN45_000144"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL0621948.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            44023..45381
                     /locus_tag="PWN45_000145"
     CDS             44023..45381
                     /locus_tag="PWN45_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL0621949.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            45898..47418
                     /locus_tag="PWN45_000146"
     CDS             45898..47418
                     /locus_tag="PWN45_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0621950.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(47787..48482)
                     /locus_tag="PWN45_000147"
     CDS             complement(47787..48482)
                     /locus_tag="PWN45_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0621951.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(48482..51139)
                     /locus_tag="PWN45_000148"
     CDS             complement(48482..51139)
                     /locus_tag="PWN45_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDL0621952.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            51277..51387
                     /gene="kdpF"
                     /locus_tag="PWN45_000149"
     CDS             51277..51387
                     /gene="kdpF"
                     /locus_tag="PWN45_000149"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDL0621953.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            51411..53087
                     /gene="kdpA"
                     /locus_tag="PWN45_000150"
     CDS             51411..53087
                     /gene="kdpA"
                     /locus_tag="PWN45_000150"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDL0621954.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            53106..55133
                     /gene="kdpB"
                     /locus_tag="PWN45_000151"
     CDS             53106..55133
                     /gene="kdpB"
                     /locus_tag="PWN45_000151"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDL0621955.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            55153..55713
                     /locus_tag="PWN45_000152"
     CDS             55153..55713
                     /locus_tag="PWN45_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDL0621956.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            55921..56400
                     /locus_tag="PWN45_000153"
     CDS             55921..56400
                     /locus_tag="PWN45_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621957.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            56393..57976
                     /locus_tag="PWN45_000154"
     CDS             56393..57976
                     /locus_tag="PWN45_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDL0621958.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            57963..58454
                     /locus_tag="PWN45_000155"
     CDS             57963..58454
                     /locus_tag="PWN45_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDL0621959.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            58458..58817
                     /locus_tag="PWN45_000156"
     CDS             58458..58817
                     /locus_tag="PWN45_000156"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL0621960.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            58883..60031
                     /gene="alr"
                     /locus_tag="PWN45_000157"
     CDS             58883..60031
                     /gene="alr"
                     /locus_tag="PWN45_000157"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL0621961.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            60116..60286
                     /gene="mazE"
                     /locus_tag="PWN45_000158"
     CDS             60116..60286
                     /gene="mazE"
                     /locus_tag="PWN45_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDL0621962.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            60283..60645
                     /locus_tag="PWN45_000159"
     CDS             60283..60645
                     /locus_tag="PWN45_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0621963.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
BASE COUNT        21366 a         8900 c        11487 g        18931 t
ORIGIN      
        1 gtcatttact attttatgta tgacatattt taaaaagtga gggtcaagca tgtcttataa
       61 agcatatcca ttctttagag atatattaat aaatgaatgt atttatttcg cctctaaaaa
      121 taaaaaacta gtacgcctaa attataaaag tgaagcgtat gtaggcgttt ggacagaaga
      181 aagtgtggcc gtatcatttt taacaagtcg tgatattcca tttgataaag ttgtaaaaat
      241 ggacgttgat cgttttgcta cttatgaatt agatgaattg tttgatgaac aagaccatat
      301 tattatgaat caaacaatgg aagaagaagg gcatctacta aacgttgtag ctgttacaca
      361 agaagtgatg acggaattag ataaaattag aatcaaagaa tttgtccaag atgtagcgaa
      421 atatgatgaa gtatacggct taactaaaaa aggtagtaag cagtttattc tcattagtga
      481 aaatgatagc gacgaaaaaa agccgcatat tatgcctgta tggagtatta aaaacagagc
      541 gttaaaagtt cgagatgaag attttgaaga gtgtgattta attacgattg aaggttctgt
      601 tttcggagaa tggctagatg aacttagaga tgatcataaa gccgttgcga tagatttaaa
      661 aactggcgtg gttggtacaa ttgtttcagc gcaaaaattg tctaatgaac ttacatttta
      721 attgaattga gttgtaagta attaagtgat aataaatgga acaatacgta ttcaaaacac
      781 ttagaccata aaataaaagg ccatttatat agcgtttatt taaaacaacg cgcatataaa
      841 tggtcttttt ctatttttct aaatataatg caccaatagc acctgaaaat gcgccgtttt
      901 caacatagta cggtttgcaa ccgcgtaaca cagtataatc ttccacaact ttgcgtaata
      961 aagcgttatt atgaaatgaa gaaccgatat aaacgatatt ttcagtttta aattcacgtg
     1021 caacagtaat ggccattgtc gtaacaactt cgccaacgac accaataacg gctgctaatt
     1081 tattgctagg tgtaaaatca gcatctaaat gatgtagtac atgaccaaaa ttagctgctg
     1141 ttaaatcacc gggaatgggt ggttcggtat ctttataaat atgtctaacc tttaaatcga
     1201 tagtgttacg atcaccgtgt tgtgccatgt cagttaactg tttataatca gtgatttgac
     1261 ttagtaaata accgagtcct tgaatcatgc ctccacctgt accgataccg cctacacgac
     1321 gttgtgattg gccgtcgaaa taatgtagtg acgtaccggt accaacattt gcaaaaatat
     1381 aatctgctaa gtcatggcct tgctctttta acaaaatacc tagtccttga gatgcagcat
     1441 caaactctac aaaaatttgt gcaggaatgt tgatgttttc agcaatgaca cctgcattac
     1501 ctccagttaa gcataatttt tcaatttgct gttggtttaa ccattccaca acttgatcaa
     1561 tatttttagt taattcagtt ttaaaagtac gttggttatc ttgctcttga acgattttaa
     1621 ttagtgtacc gccagcgtca atgccaactt tcataagatt cccacctcat tattaatgtc
     1681 tatccttaaa taatagtata gtaaaatgac taaaaaacaa gtaataatag taattattaa
     1741 caaatttgat gccattgcat ttcaacattg taagcgtatc gcaattaatg ttttacaaac
     1801 gtggacgtta agttatatat attattttct aggaattttg aagttgtata ggattgttag
     1861 ttagtgacgc aatattaaaa gtagttcgta cgcagtgtat ttgtaagtct ctgattaaaa
     1921 tgataagtaa tgaggaatag tacattaatt ttgaaattta aaaaatataa ataagtaatt
     1981 tatttaactt agagcaaata atggtatcgt agtgaaataa taggtaaaat aatatgggga
     2041 ttcatgcttc atatataaaa agataggggt taaatatatg gctaaagaac tttgttttga
     2101 aggtatcact ttaaaagcat ttgatgaaca atatcgttca gcaattaatg attttgactt
     2161 gaatgaaaga caacaaatat attcatcttt acctaaagaa gttattgatg atgcaattaa
     2221 tgatgctgat aggattgcta acgtagcaat aaacgataaa aatgaagtgg tgggcttttt
     2281 tgtattacat cgttactatc agcatgaagg ttatgataca cctgaaaatg tcgtttatat
     2341 tcgttcatta tcgattaatg aaaaatatca aggttttgga tatggcacga aaataatgat
     2401 gtcattgccg caatatgttc aaggtgtatt tcctgatttt aatcatctat atctagtagt
     2461 agatgcggaa aatgacaatg cttggaacct atacgaacgt gctggattca tgcatacagc
     2521 gactaaagaa gaaggaccaa ttggcaaaga acgactatat tacttggact tagattcaaa
     2581 acatgtttca tcattaaagc ttgaagaaga aagtcgttca gaagtgacca atgtacatat
     2641 cattaattta atgattgatg gccaaaaggt tggctttatc gcattggagc agattggtga
     2701 acgcatgaac attgctgcta ttgaagtgga taaatcatat cgctttaatg gtattggttc
     2761 aagtgctctg cgacaattgc caacttactt aagaaaaaac tatgacaacc ttaatgtgat
     2821 tacgatgatt ctgtttggag agaataatga ttttaaacca ttatgtttaa atagtaattt
     2881 cgttgaaatc gaacaaactg atgattatgt cgttttcgaa aaatatttaa attactaaca
     2941 gtgattgcga aatatgatat tgtcatttat aatttagttt tgttactata tataaatgaa
     3001 ttcagacgta taaatttaga ttatatcctt cgaaaggaag tattgggcaa tgaaaattca
     3061 agattataca aaacaaatgg ttgatgaaaa atcatttatt gatatggctt atacattatt
     3121 gaatgataaa ggcgaaacaa tgaacttata tgatatcatc gatgaattta gagcgttagg
     3181 tgattatgag tacgaagaaa ttgaaaatcg tgttgtacaa ttttacacgg atttaaacac
     3241 agatggtcgt tttttaaatg ttggagaaaa tttatgggga ttacgtgatt ggtattcggt
     3301 agatgatatt gaagagaaaa tcgcaccaac tattcaaaaa ttcgatattc tggatgcaga
     3361 tgatgaagaa gatcaaaact taaaattatt gggcgaagat gaaatggatg acgacgatga
     3421 tattccagct caaacagatg atcaagaaga actaaatgat ccagaagatg agcaggttga
     3481 agaagaaatc aatcattcgg atatagtcat tgaagaagat gaagatgaac tagacgaaga
     3541 cgaagaagtg tttgaagacg aagaagactt caacgattaa ttttttgttt gacttttagt
     3601 tgaaagatga taaaatttta ttcgggctcc tttaaatagg acacgtgtat aaaatttata
     3661 cgctcccctt acagaatttg tgagagggag cgttttttta tttaattgag taaatcaaga
     3721 aatgataacg caaaaatcaa agttgtaaat gatatacata gtgacatagc agtatggaaa
     3781 cggtaagtaa acagaattta attttgtcga attcgacaaa taaacaactt gaatgagctt
     3841 gctttaatgt tatgttttac gtaattttta caattgatga ggaagcattc cctttaataa
     3901 ttaggaggtc aagacatgac aaaatttatt tttgtaacag gtggcgtagt ttcatcatta
     3961 gggaagggta ttacagcatc ttctctaggt agattattaa aagatagagg tctaaatgta
     4021 acaattcaaa aattcgatcc atacttaaat gttgacccag gtacaatgag tccttatcaa
     4081 catggtgaag tattcgtaac ggatgatggt gcagaaactg acctagactt aggacattac
     4141 gaaagattta ttgatattaa tttaaacaag ttttcaaatg tgacagccgg taaagtgtat
     4201 tcacacgtat tgaaaaaaga acgtcgtggt gattacttag gcggaacagt tcaagttatt
     4261 ccgcatatta caaatgaaat taaagaacgt ttattacttg caggggaaag tacgaatgca
     4321 gacgttgtta tcactgaaat tggcggtaca acaggtgata ttgagtcatt accgtttatt
     4381 gaagcgattc gtcaaattcg tagcgattta ggtagagaaa atgttatgta tgttcactgt
     4441 acattactgc cttatattaa agctgctgga gaaatgaaaa cgaagccaac acaacatagt
     4501 gttaaagaat tacgaggctt aggtattcaa ccagacttaa tcgttgtaag aactgaatat
     4561 gaaatgacac aagatttaaa agataaaatt gcattattct gtgacattaa taaagaaagt
     4621 gttattgaat gtcgtgatgc agactcttta tacgaaattc cattacaatt aagccaacaa
     4681 aatatggatg atatcgttat taaacgttta caattaaacg cgaaatatga aacacagctt
     4741 gatgaatgga aacagttgtt agatatcgtt aataatttag atggtaaaat tacaattggt
     4801 ttagtaggta aatatgttag cttacaagat gcatatttat cagttgttga atcattgaaa
     4861 catgctggat atccttttgc caaagatatt gacattagat ggattgattc aagtgaagta
     4921 acagatgaaa atgcagccga ataccttgca gatgtcgacg gtattttagt accaggtgga
     4981 tttggtttcc gtgcaagtga aggtaaaatt agtgcaatta agtatgctag agaaaacaat
     5041 gtaccattct ttggtatttg tttaggaatg caacttgcaa cagttgaatt ttcaagaaac
     5101 gtattaggcc ttgaaggcgc acattcagct gaattagacc cagcaacacc atacccaatt
     5161 atagatttat taccagaaca aaaagatatc gaagatttag gtggtacatt acgcttaggc
     5221 ttatatccat gttcaattaa agaaggcaca ttggcacaag atgtttatgg taaagcggaa
     5281 attgaagaaa gacatcgtca tcgttatgaa tttaataatg actatagaga acaattagaa
     5341 gcaaatggta tggtgatttc tggtacaagt ccagatggac gtttagtaga aatggtagag
     5401 attccgacaa atgatttctt tattgcttgt caattccacc cagaattctt atctagacca
     5461 aatcgtccgc acccgatttt taaatcattt attgaagctt cattaaaata tcaacaaaat
     5521 aaataaattt gctaataaaa ccggtacttt cattgttaaa cattgaaagt accggttttt
     5581 cgtataattt taatattatg ttagtgacaa ggtatgaaat aacaatagtg acttttataa
     5641 ttctaagtct cttgtcattt caatcatttg tgtataaatg tcatagtata cataattcaa
     5701 tgccatcgca tgtggttgga caatcttatc gtaatcttca gtgtagacta taggtcttgg
     5761 tgtagataaa tcgataaaat gtacgagatg atcagggaaa tcatctgttt taggtttgtt
     5821 gcttattaag accacatcga tatctaagtc gataagtttt tgaatatcta atgcaacttg
     5881 atcattataa aatggtgcga ataataatac acgatcagtt gagtcaattt ctttaaagtc
     5941 tttaatagcg tcaagttttc ggctagattt taaacgctca tcactatgta gaatgaaact
     6001 ttcgaaaaac tgtaaatcat cataaccttt tacataaaca taaccttcgc caccaattgc
     6061 ttgaattaaa cattgggcgg ccatttgaat ttctaaagat tgtttttcta gcctattaaa
     6121 gatacctatt agttgtgtgt ttaagatttt tgacatcttt atcctccaat ctacttataa
     6181 aatattgtaa ttaatgacta catattatgc aacggcttaa attgtataaa aatgtatacg
     6241 tttgcattta gtataactat cgcatttttc aaaaaataca catttaatct gcagtatttc
     6301 aatgcattga cgctattttt ttgatataat tactttgaaa aatacgtgcg taagcactca
     6361 aggaggaact ttcatgcctt tagtttcaat gaaagaaatg ttaattgatg caaaagaaaa
     6421 tggttatgcg gtaggtcaat acaatattaa taacctagaa ttcactcaag caattttaga
     6481 agcgtcacaa gaagaaaatg cacctgtaat tttaggtgtt tctgaaggtg ctgctcgtta
     6541 catgagcggt ttctacacaa ttgttaaaat ggttgaaggg ttaatgcatg acttaaacat
     6601 cactattcct gtagcaatcc atttagacca tggttcaagc tttgaaaaat gtaaagaagc
     6661 tatcgatgct ggtttcacat cagtaatgat cgatgcttca cacagcccat tcgaagaaaa
     6721 cgtagcaaca actaaaaaag ttgttgaata cgctcatgaa aaaggtgttt ctgtagaagc
     6781 tgaattaggt actgttggtg gacaagaaga tgatgttgta gcagacggca tcatttatgc
     6841 tgatcctaaa gaatgtcaag aactagttga aaaaactggt attgatgcat tagcgccagc
     6901 attaggttca gttcatggtc catacaaagg tgaaccaaaa ttaggattta aagaaatgga
     6961 agaaatcggt ttatctacag gtttaccatt agtattacac ggtggtactg gtatcccgac
     7021 taaagatatc caaaaagcaa ttccatttgg tacagctaaa attaacgtaa acactgaaaa
     7081 ccaaatcgct tcagcaaaag cagttcgtga cgttttaaat aacgacaaag aagtttacga
     7141 tcctcgtaaa tacttaggac ctgcacgtga agccatcaaa gaaacagtta aaggtaaaat
     7201 taaagagttc ggtacttcta accgcgctaa ataattaata tttagtcttt aagttattaa
     7261 taacgtaggg atattaattt taaaagaagc agacaaaatg gtgtttgctt cttttttatg
     7321 tcgtataagt aataaataaa acagtttgat tttaaaatga aagcgtaaaa atggtaaaat
     7381 atatcaaaat tgattgtgat ataattataa ggaaaatgag caatttatga aaaaagttta
     7441 cgtgaaaacc gtagaattaa aactaaataa ttgtcaaaac aacgtcaata tttagttgaa
     7501 taatcagact ttagaccatg gtcaagtggg gaagaacagc atatattagt aaaggtgaat
     7561 gatttgttat taatcaatcg aaaatagaaa gacaagattt taacgattaa aataaactat
     7621 tttacaaata aagtaaaatt aatttataat gctaataatg caaaaaatta aaaagtaatg
     7681 gacaaaggag ataaatgata tggctcaaga ggtaataaaa ataagaggtg gacgcacact
     7741 aaatggggaa gttaatatca gtggcgcgaa aaacagtgct gtagctatca ttcctgcaac
     7801 attattagct caaggacatg tgaaattaga agggttaccg caaatctctg atgttaaaac
     7861 tttagtgagt ttattagaag atttaaatat taaggcatca ctaaatggta cggaattaga
     7921 agttgataca actgaaatac aaaatgctgc attaccaaat aataaagttg aatcattacg
     7981 tgcttcatac tatatgatgg gtgccatgtt aggtagattt aaaaaatgtg tgattggttt
     8041 accgggagga tgtccacttg ggccacgtcc aattgatcaa catattaaag gatttaaagc
     8101 tttaggtgct gaaattgatg aatcaagtac gacatcaatg aaaatcgaag ctaaagagtt
     8161 aaaaggtgca catatttttc tagatatggt tagcgtaggt gcaacaatta acattatgct
     8221 agctgcagtt tatgcaacag gacaaactgt aattgaaaat gctgctaaag aaccggaagt
     8281 agttgatgtt gctaacttct taacaagtat gggagctaat attaagggag cgggtacatc
     8341 aacaattaaa atcaatggcg tcaaagaatt acatggttct gagtatcaag ttatacctga
     8401 tagaattgaa gcaggcacat atatgtgtat cgctgcagca tgtggagaaa atgttatatt
     8461 aaataatatt gttccgaaac atgtagaaac attaactgct aaatttagtg aattaggtgt
     8521 gaatgttgat gtaagagacg aaagaattcg catcaataat aatgcgccat atcaatttgt
     8581 tgatattaaa acactagtat atccaggttt tgctactgat ttgcaacagc ctattacacc
     8641 attattattt atggcaaatg gtccttcatt tgtaactgat acgatttatc cggagcgttt
     8701 taaacatgtt gaagaattaa agcgcatggg tgcaaatatt gaagttgacg aaggcacagc
     8761 aacgataaaa ccatcaacat tacatggtgc tgaagtttat gctagcgatt taagagcagg
     8821 agcttgttta attattgctg gtttaattgc tgagggtgta actacaattt acaatgttaa
     8881 acatatttat agaggttata cagatattgt agaacactta aaagctttag gtgcagatat
     8941 ttggacggaa actgtataga tttatcttgt tttgtggtat aataattata ctgattaaaa
     9001 aatggtgttc gtttacattc taaatttatt gatgagaggt gaagatgatg gaagtatgtc
     9061 cgtatctcga agaaactttt aaaatacttg gtagaagttg gaatggatta atcattaatt
     9121 atctctcaag atgtaatgac tgttcagcac acttttccga tatgaaaaga gatttgaaaa
     9181 caataacacc acgtgcttta agtcttaagt tgtcagagct tgcacaatgg gagttagttg
     9241 agaagcaaat catttctacg agtccagtac aaattattta tgtgctaact gaaaaaggta
     9301 aagcgttagc agaggcttta catccaattg aagcatgggc gcaatcatat gtcgatttaa
     9361 cagatcaacg tactgctaaa taattacatg acgtttaaac atagcattga ttataactat
     9421 ttctaagtct tcgcattatt tgcgatgatg tgggaatagt tatttttatt taaaaatata
     9481 aaaaaataga tgcagcaaaa ttttaaagca ttttattttg aacatattaa aagggagcgt
     9541 atcataatgg aatgtaatgt ttatatcgta tgcattacgg ataaataata tataaatcat
     9601 tcttgaggag tgaaagaata atgagagact acacaaagca atacattaat ggcgaatggg
     9661 tagaaagtaa tagtaatgaa acgatagaag ttataaatcc agcaaccgaa gaagtaatcg
     9721 ggaaagttgc taaaggtaat aaagctgatg ttgataaagc cgtcgaggcg gcagacgatg
     9781 tttatttaga gttccgtcat acatctgtga aagaaagaca agcgttatta gataaaattg
     9841 taaaagaata tgaaaacaga aaagacgata ttgtacaagc tattacggat gaattaggtg
     9901 ctcctttatc attatctgag cgtgtccatt atcaaatggg actaaaccat tttgttgcag
     9961 cgagagacgc attagataac tacgaatttg aagaacgccg cggagatgat ttagttgtta
    10021 aagaagcaat cggtgtatct ggattaatta caccgtggaa cttccctaca aaccaaacat
    10081 cattaaaatt agcagcagca tttgcggctg gtagtccagt tgtacttaaa ccatctgaag
    10141 aaacaccatt tgcagctgtt attttagctg agatttttga taaagtcggt gttcctaaag
    10201 gtgtatttaa ccttgttaat ggtgatggtg ctggtgttgg gaatccttta tctgaacatc
    10261 ctaaagtacg catgatgtca tttacaggat caggccctac tggttctaaa attatggaaa
    10321 aagccgctaa agattttaaa aaggtatcat tagagcttgg tggcaaatca ccatatatcg
    10381 tcctagatga cgtagatatt aaagaagcgg ctaaagcaac aacaggcaaa gttgttaata
    10441 atactggtca agtatgtaca gctggtacac gtgttttagt gcctaacaaa attaaagatg
    10501 cattcttagc tgaattaaaa gaacaattta gccaagtgcg tgtcggtaat ccaagagaag
    10561 atggtacaca agtaggccct atcattagta aaaaacaatt tgatcaagta caaaattata
    10621 ttaataaagg tattgaagaa ggtgctgaat tattttatgg tggtcctggt aaaccagaag
    10681 gacttgaaaa aggatacttt gcacgtccga caatttttat taatgtagat aatcaaatga
    10741 cgatagcaca agaagaaatt tttgggccag taatgtcagt tatcacttat aacgatttag
    10801 atgaagcgat tcaaattgca aatgatacaa aatatggttt ggcaggatat gttattggta
    10861 aggacaaaga aacattgcat aaagtagctc gttctattga agcaggtaca gtagaaataa
    10921 acgaagcagg tagaaagcca gatttaccat ttggtggcta taaacaatct ggtttaggtc
    10981 gtgaatgggg cgattatggt attgaagagt tcttagaagt gaaatctata gctggatatt
    11041 ttaaataaat ttgatttttg aattaaaaat cgcaataaaa cagtgcacat gactaattaa
    11101 gttttgtgta ctgttttaat tttgcaattt ttataaatag attttgtaat taaaataaaa
    11161 atttgctata gttattcatg tatttaaaag gttggggatt agcataatgg gattgtgcta
    11221 gcacagttat ttatgcattg tcatgcctat ctattactta ctaactaaaa aataatgaaa
    11281 tgggtgtaaa ctatatgcct gaaagagaac gtacatctcc tcagtatgaa tcattccacg
    11341 aattgtacaa gaactatact accaaggaac tcactcaaaa agctaaaact cttaagttga
    11401 cgaactatag taaattaaat aaaaaagaac ttgttctagc tattatggaa gcacaaatgg
    11461 aaaaagatgg taactattat atggaaggta tcttagatga tatacaacca gatggttatg
    11521 gttttttaag aacagtgaac tattctaaag gggaaaaaga tatttatata tctgctagcc
    11581 aaattcgtcg ttttgaaatt aaacgtgggg ataaagtaac tgggaaagtt agaaaaccta
    11641 aagataacga aaaatattat ggcttattac aagttgactt tgtcaatgac cataacgcag
    11701 aagaagtgaa gaaacgtccg catttccaag ctttgacacc actttatcca gatgagcgta
    11761 ttaaattaga gacagaaaca caaaattatt caacgcgcat catggattta gtaacaccga
    11821 ttggtttagg tcaacgtggt ttaatagtgg cgccacctaa agcaggtaaa acatcgttat
    11881 taaaagaaat agcgaatgca atcagtacga acaaaccaga tgcaaagcta tttattttgt
    11941 tagttggcga gcgtcctgaa gaggtaacag atttagaacg ctcagtagaa gctgctgaag
    12001 tcgttcattc aacgtttgac gaaccaccag aacaccatgt taaagtagct gaattattac
    12061 ttgaacgtgc aaagcgttta gtagaaattg gggaagatgt cattatttta atggattcta
    12121 taacgagatt agcacgcgct tataacttag ttattccacc aagtggtcgt acattatcag
    12181 gtggtttaga tcctgcatct ttacacaaac caaaagcatt cttcggtgca gcgagaaata
    12241 ttgaagcggg tggaagttta acaatacttg caactgcatt agttgatacg ggttcacgta
    12301 tggacgatat gatttacgaa gaatttaaag gaacaggtaa catggagtta catttagatc
    12361 gtaaattgtc tgaacgtcgt atcttccctg caattgatat tggcagaagt tcaacgcgta
    12421 aagaagaatt gttgataagt aaatctgaat tagacacatt atggcaatta agaaatctat
    12481 tcactgactc aactgacttt actgaaagat ttattcgcaa acttaaaagg tctaagaata
    12541 atgaagattt cttcaagcag ctacaaaagt ctgcagaaga aagtactaaa acgggtcgac
    12601 ctataattta ataaacatta tataggggct tgcgttttga attaattacc tttataatta
    12661 cacagtattg ggtaaaaact cacaaataac tctgttccag atggttcagg gcaaaggagc
    12721 tgaaaataat gaaacaagga attcatcctg agtatcacca agtaatcttt ttagatacta
    12781 ctacaaactt caaattctta agcggttcta caaaaacatc ttcagaaatg atggagtggg
    12841 aagatggaaa agaataccca gttattcgtt tagatatttc atctgattca catccattct
    12901 acactggacg tcaaaaattc gctgctgcgg atggacgtgt ggaacgtttc aacaaaaagt
    12961 ttggtcttaa atcaaacaac taatatagta atcgttaagc tgtctgcatc ctataatgga
    13021 attgtagaca gcttttttat ttataagcgt agtaagggta tatacttacg actgtgttca
    13081 aagaagtgac tcaataccat gttgttaata cataatgata aatttccccc tttattaaag
    13141 tttgtattga gtagaaacat cgcacgaata agaagcaaca aattagaata gctgtatata
    13201 aatatgcaag acaaatttat aaaataattg ttcatgtaca atatcgatat aattatgaaa
    13261 aagacattta tgaatatttg aaatatgata taataaagcg attatgtttt gaaaaaggtg
    13321 gaactctcaa tgtatgaaac ttaccattcc ggttggatag aatgtattac aggtagtatg
    13381 tttagcggta aatccgaaga actaataaga cgtctaagaa ggggtatata cgctaaacaa
    13441 aaagtcgttg tatttaaacc ggcgattgat gatcgatatc ataaagaaaa agtggtctct
    13501 cataatggta atgccattga agcgattaat atttctaaag caagtgaaat tatgacgcac
    13561 gacttaacta atgttgatgt tataggaata gacgaagtgc aattttttga cgatgaaatt
    13621 gtaagtatag tagaaaaact atcagcagat ggacaccgtg ttattgttgc tggattagat
    13681 atggatttta ggggcgaacc gttcgaacca atgcctaaat tgatggctgt cagtgaacaa
    13741 gtaacgaaac ttcaagcagt ttgtgctgtt tgtggttctt catctagtag aacgcaacga
    13801 ttaataaatg gtaaaccagc taaaatcgat gatccgatta tcttagtagg tgccaatgaa
    13861 agttatgagc cacgttgtcg cgcgcaccat atcgtagcac ctagtgataa taataaggag
    13921 gaattataag tgtttgatca attagatatt gtagaagaaa gatacgaaca gttaaatgaa
    13981 ctgttaagtg acccagatgt tgtaaatgat tcagataaat tacgtaaata ttctaaagag
    14041 caagctgatt tacaaaaaac tgtagatgtt tatcgtaact ataaagctaa aaaagaagaa
    14101 ttagctgata ttgaagaaat gttaagtgag actgatgata aagaagaagt agaaatgtta
    14161 aaagaggaga gtaatggtat taaagctgaa cttccaaatc ttgaagaaga gcttaaaata
    14221 ttattgattc ctaaagatcc taatgatgac aaagacgtta ttgtagaaat aagagcagca
    14281 gcaggtggtg atgaggctgc gatttttgct ggtgatttaa tgcgtatgta ttcaaagtat
    14341 gctgaatcac aaggattcaa aactgaaata gtagaagcgt ctgaaagtga ccatggtggt
    14401 tacaaagaaa ttagtttctc agtttctggt aatggcgcgt atagtaaatt gaaatttgaa
    14461 aatggtgcgc accgcgttca acgtgtgcct gaaacagaat caggtggacg tattcatact
    14521 tcaacagcta cagtggcagt tttaccagaa gttgaagatg tagaaattga aattagaaat
    14581 gaagatttaa aaatcgacac gtatcgttca agtggtgcag gtggtcagca cgtaaacaca
    14641 actgactctg cagtacgtat tacccattta ccaactggtg tcattgcaac atcttctgag
    14701 aagtctcaaa ttcaaaaccg tgaaaaagca atgaaagtgt taaaagcacg tttatacgat
    14761 atgaaagttc aagaagaaca acaaaagtat gcgtcacaac gtaaatcagc agtcggtact
    14821 ggtgatcgtt cagaacgtat tcgaacttat aattatccac aaagccgtgt aacagaccat
    14881 cgtataggtc taacgcttca aaaattaggg caaattatgg aaggccattt agaagaaatt
    14941 atagatgcac tgactttatc agagcagaca gataaattga aagaacttaa taatggtgaa
    15001 ttataaagaa aagttagatg aagcaattca tttaacacaa caaaaagggt ttgaacaaac
    15061 acgagctgaa tggttaatgt tagatgtatt tcaatggacg cgtacggact ttgtagtcca
    15121 catgcatgat gatatgccga aagcgatgat tatgaagttc gacttagcat tacaacgtat
    15181 gttattagga gagcctatac agtatatagt tggctttgcc tcattttatg gtagaacgtt
    15241 tgatgtaaac tcaaattgtt tgataccaag acctgaaact gaagaagtaa tgttgcattt
    15301 cttacaacag ttagaagatg atgcaacaat cgtagatatc ggaacgggta gtggtgtact
    15361 tgcaattact ttgaaatgtg aaaagccgga tttaaatgtt attgctactg atatttcact
    15421 tgaagcaatg aatatggctc gtaataatgc tgagaagcat caatcacaaa tacaattttt
    15481 aacaggggat gcattaaagc ccttaattaa tgaaggtatc aagttgaacg gcttgatatc
    15541 taatccacca tatatagatg aaaaagatat ggttacgatg tctccaacgg ttacgagatt
    15601 cgaaccacat caggcattgt ttgcagataa ccatggatat gctatttatg aatcaatcat
    15661 tgaagattta cctcacgtta tggaaaaagg cagcccagtt gtttttgaaa ttggttacaa
    15721 tcaaggtgag gcacttaaat caataatttt aaataaattt cctgacaaaa aaatcgacat
    15781 tattaaagat ataaatggcc acgatcgaat cgtctcattt aaatggtaat tagaagttat
    15841 gcctttgcta tgattagtta agtgcatagc tttttgcttt atattatgat aaataagaaa
    15901 ggcgtgatta agttggatac taaaatttgg gatgttagag aatataatga agatttacag
    15961 caatatccta aaattaatga aataaaagac attgttttaa acggtggttt aataggttta
    16021 ccaactgaaa cagtttatgg acttgcagca aatgcgacag atgaagaagc tgtagctaaa
    16081 atatatgaag ctaaaggccg tccatctgac aatccgctta ttgttcatat acacagtaaa
    16141 ggtcaattaa aagattttac atatactttg gatccacgcg tagaaaagtt aatgcaggca
    16201 ttctggccgg gccctatttc gtttatattg ccgttaaagc taggctatct atgtcgaaaa
    16261 gtttctggag gtttatcatc agttgctgtt agaatgccaa gccattctgt aggtagacaa
    16321 ttattacaaa tcataaatga acctctagct gctccaagtg ctaatttaag tggtagacct
    16381 tcaccaacaa ctttcaatca tgtatatcaa gatttgaatg gccgtatcga tggtattgtt
    16441 caagctgaac aaagtgaaga aggattagaa agtacggttt tagattgcac atcttttcct
    16501 tataaaattg caagacctgg ttctataaca gcagcaatga ttacagaaat acttccgaat
    16561 agtatcgccc atgctgatta taatgatact gaacagccaa ttgcaccagg tatgaagtat
    16621 aagcattact cacccaatac accacttaca attattacag atattgagag caaaattgga
    16681 aatgacggta aagattggtc ttctatagct tttattgtgc cgagtaataa ggtggcgttt
    16741 ataccaagtg aagcgcaatt cattcaatta tgtcaggatg acaatgatgt taaacaagca
    16801 agtcataatc tttatgatgt gttacattca cttgatgaaa atgaaaatat ttcagcggcg
    16861 tatatatacg gctttgagct gaatgataat acagaagcaa ttatgaatcg catgttaaaa
    16921 gctgcaggta atcacattat taaaggatgt gaactatgaa gattttattc gtttgtacag
    16981 gtaacacatg tcgtagccca ttagcggaaa gtattgcaaa agaggttatg ccaaatcatc
    17041 aatttgaatc aagaggtata ttcgctgtga acaatcaagg tgtttcgaat tatgttgaag
    17101 acttagttga agaacatcat ttagctgaaa cgaccttatc gcaacaattt actgaagcag
    17161 atttgaaagc agatattatt ttgacgatgt cgtattcgca caaagaatta atagaggcac
    17221 actttggttt gcaaaatcat gttttcacat tgcatgaata tgtaaaagaa gcaggagaag
    17281 ttatagatcc atacggtgga acaaaagaaa tgtatgtaca tacctatgaa gaacttgtaa
    17341 gtttaatttt aaaattaaaa gatattattt gctagtagga attctgtata attagtatat
    17401 gttaacagtt aaataagtta taatagagga gtgtagcact ttgctatatt ccttttttat
    17461 ttttaggaag ggtgggcttt tatgaaagat ttgacaatgt tattagacga attaaaagat
    17521 atgtcctttt ttaataaagg agatatatgt ttaattggat gttcaacatc ggaagtcatt
    17581 ggcgaaaaaa tagggactgt cggttcgatg gaagttgcag aaactatttt taatgcatta
    17641 gatgttgtga gtaaagagac aggcgttact tttgcttttc aaggatgtga acatatcaac
    17701 agagcgataa caatagaaaa atctcagtat aatccattaa caatggaaga agtttccgtc
    17761 gttccagatg tacatgctgg agggagtttg gcaacatacg cttttcagca tatgaaggat
    17821 ccgatcgtgg tcgaacatat tactgttcct tgtggaattg atattggtca aacgttaata
    17881 ggcatgcata ttaagcatgt atgtgtacct gtaaggacct cagtaaagca agtaggacag
    17941 gcaattgtaa ctattgccac atctaggccg aaaaagattg gtggcgaacg tgctaaatac
    18001 caataagttt ttttgaaaga aggggagatt ttatgtctta tatcaccaag caagataaag
    18061 ttatcgcaga agcaatcgag agagaatttc agagacaaaa tagcaacata gagttaatcg
    18121 catcggaaaa ttttgtatcg gaagcggtta tggaagcaca aggttcagtg ttgactaata
    18181 agtatgctga aggctatcca ggacgccgat attatggtgg ctgtgagttt gtagatgtta
    18241 ctgaaagcat cgcaattgat cgtgctaaag cattgtttgg agctgaacat gtcaatgttc
    18301 aaccacattc aggttcacaa gcgaacatgg ctgtttactt agttgcatta ggaatgggcg
    18361 acacagtttt aggtatgaat ttgagtcatg gtggtcactt gacacatgga gcgcctgtta
    18421 attttagtgg taaattctac aatttcgttg aatatggagt agataaagac acagaacgaa
    18481 tcaattatga tgaagttcgt aaattagcgt tagagcataa gcctaagctt attgtggcag
    18541 gagcatcagc atattcaaga acaattgact tcaaaaagtt taaagaaatc gcagatgaag
    18601 taaacgctaa gttaatggta gacatggcac atattgcagg attagtagcg gcaggtttac
    18661 atccaaatcc agtagaatat gctgattttg taacaactac aacacacaaa acattacgcg
    18721 gaccacgtgg tggtatgatt ttatgtaagg aagaatataa aaaagacata gataaaacaa
    18781 ttttccctgg tattcaaggt ggacctcttg agcatgttat tgcagcaaaa gcagttgctt
    18841 ttggagaagc gttagaaaat aatttcaaaa cgtatcaaca acaagtggtt aaaaacgcaa
    18901 aagttcttgc agaagcatta attaatgaag gatttagaat tgtttctggc ggtacagata
    18961 atcacttagt agctgttgat gtaaaagggt ctataggact tactggtaaa gaagctgaag
    19021 agactttaga ttcagttggt atcacatgta acaaaaatac cattccgttc gatcaagaaa
    19081 aaccttttgt aacgagtggt atacgtttag gtacacctgc tgcaacaacg cgtggatttg
    19141 atgaaaaagc ttttgaggaa gttgcaaaaa tcatcagttt agcattgaaa aatagtaaag
    19201 atgaagaaaa attacaacaa gctaaagaac gcgttgcgaa attaacagct gaatatcctc
    19261 tatatcaata aacatacaca aagataggag taatttttat gagtaaagta cacgttttcg
    19321 atcatccatt aattcaacac aaactaagct atattagaga tgtaaataca ggtactaaag
    19381 aatttagaga acttgtagac gaagttggta tgttgatggc atatgaagta actagagact
    19441 tagagcttca ggatgttgat attgaaacgc ctgtaactaa aatgacggct aaacgattag
    19501 ctggtaaaaa attagctatc gttccaattt taagagctgg tttaggtatg actgatggta
    19561 ttttaagttt agtacctgca gccagagttg gacatatcgg tttatatcga gatcccgaaa
    19621 cattaaaggc tgtagagtat tttgctaagt tacctcaaga tattactgaa agacaaatca
    19681 ttgttgttga tcctatgtta gcaacaggcg catcagcaat tgaagctatt acttcattga
    19741 agaaacgtgg cgctaaaaat attcgcttca tgtgtttaat tgcagcacca gaaggcgtag
    19801 aaaagatgca tgaagcacat ccagatgtag atatttatat tgctgcactt gatgaaaagc
    19861 taaatgacaa agcatatatc acaccagggt taggcgacgc tggagataga ttgtttggta
    19921 ctaaatagaa gaatcggaga taagtagttt gatgaaaaag attatgacca tatttggaac
    19981 aagacccgaa gccataaaga tggctccttt agttaaagct ttggaacaag aaaaaatgct
    20041 cgagccaatt gttgttgtta cagcacagca tagagaaatg cttgattcag tattaagtac
    20101 ctttgaaatc aaaccaaaat acgatttgaa tattatgaaa tcaggacaga cactttctga
    20161 gataacttct aaatcaatca ctcaattaga acaagtgatt caattagaaa aaccagacat
    20221 ggtacttgta catggtgata cgatgacgac atttgcaggt ggtttagcgg cattttataa
    20281 ccaagtgccg attggacacg ttgaagcagg gcttagaagt tatgataaat attcaccttt
    20341 tccagaagag gtgaatagac aattagttgg tgtattagct gacttacatt ttgcacctac
    20401 taaaaatgct gcatcgcatt tgttaagtga agggaaatat tcagaaagtg ttgttgtcac
    20461 tggtaatact gcgattgatg ctatgaaata tacagttgat gacaattata aatctaacat
    20521 catggataaa taccatgaca aaaaattcat tcttatgaca gcacaccgac gagaaaatat
    20581 cgggaaaccc atggaaaata tatttaaagc ggttagacgc ttaatagatg agtatactga
    20641 tttagcactg gtatatccaa tgcataaaaa cccgaaagtt cgagaggtgg ctcaaaagat
    20701 tttaggtagt catgacagaa ttgaattgat tgaacctttg gacgtagtag atttccataa
    20761 ttttgctaaa aaatcttatt ttattttgac agattcaggt ggaattcagg aagaagcgcc
    20821 atcatttaat aagccagttt tagttttgcg aagtgttaca gaacgtcctg aaggtgttga
    20881 agctggcaca ttaaaagtca ttggaacaaa taagcaaaat gtgtatcaag ctgcaaaaga
    20941 attaatcgat gatgagagat tatatcatca aatgtctgaa gcatctaatc cttacggtga
    21001 tggatttgct tcagaaagaa tagttaatca tattaagtat tatttgaatt taatcactga
    21061 aaaaccaagc gatttctgag gaaaatagac tttttgtcta attgtgtcta aattgtgtct
    21121 aactatgctt atttttttac aacaatttga atagaattga caccacaaat cgcctatatt
    21181 atcatttagt ttgtatgagt ggaacggttt tcatgattga tatcagtcac acggaggcat
    21241 ataaaatgag tcgtttctac aacattttta aacagttcat tcaatattat ttttatctaa
    21301 taataatatt gggaggatta tacctttata cacaccatgc atttatctta ggattaatca
    21361 ttggtgttac tggttctgca atcaacacat gtatttttga atgctattta gctaaagcta
    21421 aaagaccaga cactatgcat atttcaactg gaaatatgtg gcgatactta gttgcaatta
    21481 ttgcctgtat gatttggtac cttaataaag cgcatgtaag tatcatcggt ataattattg
    21541 gtttaatgat ttcatatgtt gtagttatca tacgtccttt actaaaggtg agcaaataaa
    21601 ttaagaaaga ggtgagatta tggatcacaa atccccgctc gtgagttgga atttattcgg
    21661 ttttgatatc gttttcaatt tatcaagtat attgatgata cttgttacgg cgtttcttgt
    21721 ttttctactt gctatcattt gtacgcgtaa tttgaaaaaa agaccaactg gcaaacaaaa
    21781 tttcgttgaa tggatttttg atttcgtgag gggaatcatt gaaggtaaca tggcttggaa
    21841 aaaaggtggt caattccact tcttagcagt aacgctgatt ctgtacattt ttatagctaa
    21901 tatgttaggt cttccgtttt ctatagtaac gaaagatcac acattgtggt ggaaatcacc
    21961 gacagctgat gcaacagtga ctttaacgtt gtctacaacg ataatactgt taactcactt
    22021 ttatggaatt aaaatgcgtg gtacgaaaca atatcttaaa ggttatgtac agccgttttg
    22081 gccattggca attattaatg tttttgaaga gttcacttca acattaacgc ttggtctgcg
    22141 tttgtacggt aacatatttg caggtgagat actattaaca ttacttgctg gcttattctt
    22201 taacgaacca gcatggggtt ggattattag tatcccagga ttaattgttt ggcaagcatt
    22261 ttcaatattt gtaggaacaa tccaagcata tatctttatt atgctttcga tggtttatat
    22321 gtcacataaa gtggcagatg aacactaaaa atttcaataa ttatatacaa tcacaggagg
    22381 aaattaaatt atgaatttaa tcgcagcagc aatcgcaatt ggtttatcag cattaggagc
    22441 aggtatcggt aacggtttaa tcgtttcaag aacagttgaa ggtgtagcac gtcaaccaga
    22501 agcacgtggt caattaatgg gtatcatgtt cattggtgta ggtttagttg aggcattacc
    22561 tatcatcggt gtagtaattg cattcatgac atttgctgga taattaacag ataaaagagg
    22621 tcgggacaaa gcgcatagga cataattcat gatgcatata tagtaatatc tttgaacttt
    22681 attaaatagt tgagatatga acgcaccatg cctatcgcat aaattcagta ggtcctaacc
    22741 tcgtcgtttt tttctatata acactagcga ttattttaat gaaaggagtg tcatgaaccc
    22801 gtgactgaaa cagctaactt attcgttctt ggtgcagctg gaggcgttga gtggggtact
    22861 gtgattgtac aggtcctaac tttcatcgtg ttacttgcgt tacttaaaaa gttcgcatgg
    22921 ggtccattga aagatgtaat ggataaacgt gaaagagata ttaacagaga tatcgatgac
    22981 gcagaacaag ctaagttaaa tgcacagaaa cttgaagaag aaaataaaca aaaacttaaa
    23041 gaaacacaag aagaagttca aaagatttta gaagatgcta aggttcaagc acgtcaacag
    23101 caagaacaaa ttattcatga agcaaacgta cgtgcaaacg gtatgattga aacagcacaa
    23161 agtgaaatca atagccaaaa agaacgtgcc attgcagata ttaataatca agtatctgaa
    23221 ctatcagtgt taattgcttc taaagttctt agaaaagaaa tttctgaaca agaccaaaaa
    23281 gcattggttg acaagtatct aaaagaggca ggcgataaat aatggtaaaa gtagctaaca
    23341 agtatgctaa agcattattt gacgtgtcat tagatacaaa taatttagag actattaatg
    23401 aagaattaac agttataaat gaagcagtaa aagataaaat tgagcaattg agaatggttg
    23461 acagtaatcc aactcaaaca gcagaacaac gtcgtgagtt gattaatggt gtatttactg
    23521 acattaatcc atacattaaa aatatgatgt acgtgttagc agataataga catatctcat
    23581 taatagctga cgtattcaag gcgttccaaa gcttatataa cggacactac aatcaagatt
    23641 ttgcaacaat tgagtcaaca tatgaattga gtcaagaaga gttagataag attgtcaaac
    23701 tagtaactca acaaacgaag ttatctaaag ttattgtaga tacaaaaatt aatccagatt
    23761 taattggtgg atttagagtt aaagtcggca caactgtatt agatggtagt gttagaaatg
    23821 atcttgtcca attacaaaga aaatttagaa gagttaatta attataaaga ggagtgacat
    23881 agatggccat aaaagctgaa gaaatcagtg cattacttcg ctcacaaatt gaaaattatg
    23941 agtcagaaat gtccgtaact gatgtaggta ctgtattaca aattggtgat ggtattgcat
    24001 taattcacgg attaaatgac gttatggctg gtgagctagt agaattccat aacggcgtac
    24061 ttggtttagc ccaaaacctt gaagagtcaa acgtgggtgt ggttatttta ggaccataca
    24121 caggtattac tgaaggtgac gaagttaaac gtactggtcg tatcatggaa gtaccagtag
    24181 gtgaagaact aatcggaaga gttgttaatc cattaggaca acctattgat ggacaaggac
    24241 cgattaacac aactaaaaca cgtccagtag agaaaaaagc tactggtgta atggatcgta
    24301 aatcagtaga tgagccatta caaacaggta tcaaagcaat tgatgcttta gtaccaattg
    24361 gtagaggtca acgtgagtta atcatcggtg accgtcaaac aggtaaaaca acaattgcaa
    24421 ttgacacaat tttgaaccaa aaagatcaag gtacgatttg tatctatgtt gctattggtc
    24481 aaaaagattc aacagtaaga gcaaatgttg aaaagttaag acaagcaggc gctttagact
    24541 acactattgt tgtagcagca tcagcttctg aaccttctcc attattatat attgcaccat
    24601 attcaggtgt aacaatgggt gaagaattca tgtttaacgg taaacatgtt ttaatcgttt
    24661 atgatgattt aactaaacaa gcagcagctt atcgtgagtt atcattatta ttacgtagac
    24721 ctccaggtcg tgaagcatac ccaggtgacg tattctactt acatagtaga ttattagaaa
    24781 gagcagcaaa attaaacgat gacttaggtg gcggttcaat tactgcatta ccaattatcg
    24841 aaacacaagc tggtgatatt tcagcttatg taccaacaaa cgttatttca attacagatg
    24901 gacaaatctt cttacaatct gatttattct tctcaggtgt aagaccagcg attaatgccg
    24961 gacaatctgt atctcgtgtt ggtggatctg cacaaattaa agcaatgaag aaagttgctg
    25021 gtacgttacg tcttgactta gcgtcataca gagaacttga atcatttgca caattcggtt
    25081 cagaccttga tgaatttact gcaagtaaat tagaacgtgg taaacgtact gttgaagtct
    25141 taaaacaaga tcaaaacaaa ccattaccag tcgaacacca agtgttgatt atttatgcat
    25201 taacaaaagg atatttagat gatattccag ttgtagatat cacacgtttt gaagacgagt
    25261 taaaccactg ggcagaatca aatgctactg aactgttaaa tgaaatcaga gaaactggtg
    25321 gcttaccaga tgctgagaag tttgacacag caattaacga attcaaaaaa agctttagca
    25381 aatctgaata ataaacaagt ttagtataag gtggtgagat agtggcttct cttaaagaaa
    25441 tagatactcg aataaaatca accaaaaaaa tgaagcagat tacgaaagcg atgaacatgg
    25501 tatcaagttc aaaacttcgt agagctgaaa aaaatacaaa acaattcaca ccatatatgg
    25561 ataaaatgca agatgcaatt actgcagttg caggggcaag tagcaataca aaccatccaa
    25621 tgctaagacc tcgtaaaatt actagaagtg gatatttagt tatcacgagt gataaaggtt
    25681 tagcaggtgc atatagtgca aacgtgctta aaaaattgat tactgatatt gaagcgaaac
    25741 atcaagatag tagcgaatac agtattgtag ttttagggca acaaggtgtt gatttcctaa
    25801 aaaatagagg ttatgacatt gagtattctc aagtagacgt acctgatcaa ccttctttca
    25861 aatctgttca agcactagct aaccatgcta tagacttata cagtgaagaa gaaattgatg
    25921 aattaaatat atactatagt cattatgtca gcgttcttga aaacaagcct acatctagac
    25981 aagtattacc attatctcaa gaggattcta gtaaggggca tggtcatttg tcttcttatg
    26041 aatttgagcc agataaagaa tctatcttaa gtgtaatctt gcctcaatat gttgagagtt
    26101 tgatttacgg aacaatatta gacgcaaaag caagtgagca tgcaacacgt atgactgcga
    26161 tgaaaaatgc cactgataat gcaactgaac ttattgatga cttatcatta gaatataaca
    26221 gagcgagaca agcagaaatt acgcaacaaa ttactgaaat tgttggtggt tccgcagcgc
    26281 ttgaataata tttaaaggag gaaaatagca tgggaattgg ccgtgtaact caagttatgg
    26341 gtcctgtaat tgatgttcga tttgaacata acgaagttcc taaaattaat aacgccttgg
    26401 ttattgatgt gcctaaagaa gaaggtacaa tacaactaac attagaagtt gcgctgcaat
    26461 taggtgacga cgttgttcgt acaattgcga tggattcaac tgatggtgtc caaagaggca
    26521 tggatgtaaa agatacaggc aaagaaatta gtgtacctgt tggtgacgaa acattaggtc
    26581 gtgtatttaa tgtactaggt gaaacaattg accttaaaga agaaattagt gattctgttc
    26641 gccgcgatcc tatccatcgt caagcaccag cattcgatga actttcaaca gaagttcaaa
    26701 ttttagaaac aggtattaaa gtagtagatt tactagcacc ttatattaaa ggtggtaaaa
    26761 tcggattgtt cggtggtgcc ggtgtaggta aaacagtatt aatccaagaa ttaattaaca
    26821 acatcgctca agagcacggt ggtatttctg tattcgccgg tgtaggtgaa cgtactcgtg
    26881 aaggtaacga tttatacttc gaaatgagtg acagtggtgt aattaagaaa acagccatgg
    26941 tattcgggca aatgaatgag ccacctggtg cacgtatgcg tgttgcatta tctggtttaa
    27001 caatggctga atatttccgt gacgaacaag gtcaagacgt attattattc atcgataaca
    27061 ttttcagatt tacacaagct ggttctgagg tatctgcatt attaggtcgt atgccttctg
    27121 cagtaggtta ccaaccaaca cttgctactg aaatgggaca attacaagaa cgtattacgt
    27181 ctacaacaaa aggatcagtt acttctattc aagcggtatt cgtacctgcc gatgactata
    27241 ctgacccagc gcctgcgaca gcgtttgccc atttagatgc aactacaaac ttagaacgta
    27301 aattaactga aatgggtatt tatccagccg tggatccatt agcgtctaca tcaagagcat
    27361 tggaaccatc aattgtaggt caagaacatt atgaagtagc tcgtgatgta caatcaacac
    27421 ttcaaaaata ccgtgaatta caagatatca ttgctatctt aggtatggac gaattatctg
    27481 atgaagataa acaaacagtt gaacgcgcac gtagaattca attcttctta tctcaaaact
    27541 tccacgtagc ggaacaattt actggtcaaa aaggttctta tgtacctgtt aagacaacag
    27601 ttgcaaactt taaagatatc ttagatggta aatatgacca tattccagaa gatgcattcc
    27661 gtttagttgg tagcatggat gatgttattg caaaagctaa agatatgggt gttgaagtat
    27721 aacaattagg aggaatggat aatgaataca ttaaacctag atattgtcac tcctaatggt
    27781 tctgtttaca atcgtgataa tgttgaactc gttgttatgc aaacaacagc tggtgagata
    27841 ggtgtcatga gtggacatat tccaactgta gctgctttaa aaacaggctt tgtaaaagtg
    27901 aaatttcacg atggaactga atatattgct gtaagcgatg gctttgttga agttagaaaa
    27961 gataaagttt caatcattgt tcagactgca gaaactgcaa gagaaattga tgttgaaaga
    28021 gctaaattag ccaaagcaag agcagagtct cacttggaaa atgatgacga caatactgat
    28081 attcatagag ccgaaagagc tttagagaga gcaaataacc gtttgcgtgt ggctgaatta
    28141 aaatagtaaa taaagggtcg aagatgtgat ttcatatctt cgaccctttt ttgaattata
    28201 ttgatttaaa gatacaaaac atgagagggg ggaaggaatt gataaagaac cattaaagat
    28261 ttatgatgta gtggttcttt atcattaaac acagctaatg tgtatttaaa aataggaata
    28321 catgagtaaa actcatgtat aagaaatact aatttctaaa gaaaaagtat ttctttatgt
    28381 tggggccccg tcaactactg ccaaatacaa cactatagag tctagacatt gatttatgtc
    28441 cgactcccaa gaatagtttt acttttttac aatcactaat agattgctaa aatcaaaatt
    28501 tccttcacca ctatctacag tcgacatttc attttttgaa attatctaca ttttttcata
    28561 ccaagatatt ttatagttat gatatttatg taaaaagaat tatatagtaa gttagcttaa
    28621 actttactaa aaacgggtat taaactttgt atcattattt aaatttttca tgtacaatgt
    28681 aatacagtaa tcttatgagg tgataaaatg gattatatcg gacaatatgc agttatccat
    28741 ttagtgttac atgttgtatg tatttgtatt gcctattggg ctttacaatc aattagatta
    28801 gatcaatttt ttaaaaaagg atacgccact caattacaag tgtgtatgat atttgttgct
    28861 attttattag gcactgcagt aagcaatttt attgtagatt tgttacaata ctcgacgcag
    28921 gtaaaatatt taataaaata agtctaactc tatgatttgt aatcaaaact agatataatt
    28981 aaataatgac ttaaaataat tttaaaatag ggaaatgtaa agtaatagga gttctaagtg
    29041 gaggatttac gatggataaa atagtaatca aaggtggaaa taaattaacg ggtgaagtta
    29101 aagtagaagg tgctaaaaat gcagtattac caatattgac agcatcttta ttagcttctg
    29161 ataaaccgag caaattagtt aatgttccag ctttaagtga tgtagaaaca ataaataatg
    29221 tattaacaac tttaaatgct gacgttacat acaaaaagga cgaaaatgct gttgtcgttg
    29281 atgcaacaaa gactctaaat gaagaggcac catatgaata tgttagtaaa atgcgtgcaa
    29341 gtattttagt tatgggacct cttttagcaa gactaggaca tgctattgtt gcattgcctg
    29401 gtggttgtgc aattggaagt agaccgattg agcaacacat taaaggtttt gaagctttag
    29461 gcgcagaaat tcatcttgaa aatggtaata tttatgctaa tgctaaagat ggattaaaag
    29521 gtacatcaat tcatttagat tttccaagtg taggagcaac acaaaatatt attatggcag
    29581 catcattagc taagggtaag actttaattg aaaatgcagc taaagaacct gaaattgtcg
    29641 atttagcaaa ctacattaat gaaatgggtg gtagaattac tggtgctggt acagacacaa
    29701 ttacaatcaa tggtgtagaa tcattacatg gtgtagaaca tgctatcatt ccagatagaa
    29761 ttgaagcagg cacattacta atcgctggtg ctataacgcg tggtgatatt tttgtacgtg
    29821 atgcaatcaa agaacatatg gcgagtttag tctataaact agaagaaatg ggcgttgaat
    29881 tggactatca agaagatggt attcgtgtac gtgctgaagg ggaattacaa cctgtagaca
    29941 tcaaaactct accacatcct ggattcccga ctgatatgca atcacaaatg atggcattgt
    30001 tattaacggc aaatggtcat aaagtcgtaa ccgaaactgt ttttgaaaac cgttttatgc
    30061 atgttgcaga gttcaaacgt atgaatgcta atatcaatgt agaaggtcgt agtgctaaac
    30121 ttgaaggtaa aagtcaattg caaggtgcac aagttaaagc gactgattta agagcagcag
    30181 ccgccttaat tttagctgga ttagttgctg atggtaaaac aagcgttact gaattaacgc
    30241 acctagatag aggctatgtt gacttacacg gtaaattgaa gcaattaggt gcagacattg
    30301 aacgtattaa cgattaattc agtaaattaa tataatggag gatttcaacc atggaaacaa
    30361 tttttgatta taaccaaatt aaacaaatta tacctcacag acagccattt ttattaattg
    30421 ataaagtagt tgaatatgaa gaaggtcaac gttgtgtggc tattaaacaa gtatcaggaa
    30481 acgaaccatt ctttcaaggg cattttcctg agtatgcggt aatgccaggc gtattaatta
    30541 ctgaagcgtt agctcaaaca ggtgcggtag ctattttaaa tagtgaagaa aataaaggta
    30601 aaatcgcttt atttgctggt attgataaat gtcgttttaa acgtcaagta gtacctggtg
    30661 atactttaac gttggaagta gaaatcacta aaattaaagg accaataggt aaaggtaatg
    30721 ctaaagctac tgtcgatggt caacttgctt gtagttgtga acttacattt gcaattcaag
    30781 atgtaaaata aaacaaaaaa aacattcaaa gatttaatgt gttggcataa tctttgaatg
    30841 ttttttattt tactcttcta atttttcatc ctttaacttt ggtttagact gcatcattcg
    30901 attaaatgat ttttttaatt cttcaccaga taatccatca tcaataagtt gttctaataa
    30961 actttcagca tactgttgac gtaaagtgta aatatgacat tcaaatacga tagacattgt
    31021 tgttttaaaa tttttaatac ttttcacagt agattcaaat aaagctggat cattggctgg
    31081 acgcgtgatg acgacacgaa tatccaataa ctgttcattg tcttgatatt ccttaaatgt
    31141 ttcataatgc tcattagaaa tgacaatttc aagcaaccaa cccgtgccac tttcctcttt
    31201 attaattatt acgccatctt ctaattcata ttctataatt ctgccgtgtt cattcacaat
    31261 ttgaaacctt actgctttaa aagttttcat attcccacat cccattagtt taatatttat
    31321 gatttttgaa ttatttagaa gatttaaggt catttcatga ctaatttctt taaatataac
    31381 aaataacgag atgctatagc tattaattat ataaaatttg ccattttgac catttttaag
    31441 ctagtaatta agatataggc ataggaggtg aatcacacac tacatgctaa ataaaatcgt
    31501 aattgtcggg agactgacga aagacgcaca aatatttgaa aaggaggata gaaaaattgc
    31561 aacgttttgt gttgcaacgc accgaaatta taaagatgaa aatggagaaa tcgtctgtga
    31621 ttacttattc tgtaaagcat ttggcaagtt agcttctaat atagaaaaat atactaatca
    31681 aggtacattg gttggtataa ctggtcaaat gagatcaaga aagtatgata aagacggaca
    31741 aacacacttt gtcactgaat tatatgttga aacaataaaa tttatgtccc ctaaatccca
    31801 aaataatgaa attctctcag atagtattta gatattgact ctcaaaatat agataatcat
    31861 gacttattag aaatttaaat ccaattatcc taaacatcct taatatacat ttaatccatc
    31921 ctaaattttt tatagatcta attaacttga aatatactca cttaaaaacg acttacatga
    31981 ctagtactgt tgtgtaggtc gtctttttgt gtagtttttc taatagaaaa tagacataga
    32041 ttgcaaaacg attacataaa aaattgcaaa taaaaaaaca tttttactgt catgacaatt
    32101 ctcgatagaa tcatatctga aattatgggg gatttcgttg taatcactgt gtaaaaaaca
    32161 aggctatttt tttattttgt tgtaacctgc acggatttta tagatgttac agtagtctct
    32221 gtaaaacttt tagagaaaca aattacttgt aggaggattt taagattatg aagaaaacat
    32281 tactcgcatc atcattagca gtaggtttag gaatcgtagc aggaaatgca ggtcacgaag
    32341 cccatgcaag tgaagcggac ttaaataaag catctttagc gcaaatggcg caatcaaatg
    32401 atcaaacatt aaatcaaaaa ccaattgaag ctggggctta taattataca tttgactatg
    32461 aagggtttac ttatcacttt gaatcagatg gtacacactt tgcttggaat taccatgcaa
    32521 caggtactaa tggagcagac atgagtgcac aagcacctgc aactaataat gttgcaccat
    32581 cagctgttca agctaatcaa gtacaatcac aagaagttga agcaccacaa aatgctcaaa
    32641 ctcaacaacc acaagcatca acatcaaaca attcacaagt tactgcaaca ccaactgaat
    32701 caaaatcatc agaaggttca tcagtaaatg tgaatgctca tctaaaacaa attgctcaac
    32761 gtgaatcagg tggcaatatt catgctgtaa atccaacatc aggtgcagct ggtaagtatc
    32821 aattcttaca atcaacttgg gattcagtag cacctgctaa atataaaggt gtatcaccag
    32881 caaatgctcc tgaaagtgtt caagatgccg cagcagtaaa attatataac actggtggcg
    32941 ctggacattg ggttactgca taagccattt atgcatagct aattaatagt tatataagta
    33001 acttttagat tggaatatat cggggaattc tcgatatgtt ccggtttttt tatttcgcaa
    33061 atatatgtca ttatagaatt attatttaaa gataataata caactgagca acaataattg
    33121 aaaatgttga aaccgtttgc taagattaat ttgcaattga atactaagga actactaggg
    33181 gagcctaatg atatggctga gatgaattgt tcagaccctt atgacctgat ttggttagta
    33241 ccaacgtagg aaagtagtta ttgaagtgat ggggctagta tagcaacatt gccatcaaac
    33301 tactttcatg ttggaagtag tttttttatt tagggaggat tttttacaat ggaattttca
    33361 caaaaattgt accaagctgc aaagccaatc attaatgata tttatgagga tgactttatt
    33421 cagaagatgt tatccggaga tataggagct gacgcattac gccattactt aaaagctgat
    33481 gctgcgtatt taaaagaatt tacaaactta tatgctttgt taattccaaa aatgaatagc
    33541 atgaatgacg taaaattttt agtagagcaa atagaattta tggttgaagg tgaagtactt
    33601 gcgcatgata ttttagcgca aattgttggt gaatcatacg aagaaatcat taaaacaaaa
    33661 gtttggcctc caagtggcga ccattacata aaacatatgt atttccaagc gcatagtcgt
    33721 gaaaatgcta tttatacaat tgctgcaatg gcaccttgtc catatattta tgcagaatta
    33781 gctaagcgct ctcaaagtga tcataagctt aatagagaaa aagatacggc aaaatggttt
    33841 gatttttata gtactgaaat ggatgacata attaacgtat ttgaggcatt aatgaataaa
    33901 ttagccgaat caatgtctga caaggaattg gaacaagtaa agcaagtatt tttagaaagt
    33961 tgcatacatg agcgtcgatt tttcaatatg gctatgacac tagaacaatg ggaatttgga
    34021 ggaaaagtaa atgattaaac ctaaaatagc attaaccatt gcaggtactg atccaacagg
    34081 tggtgccggc gtaatggctg atttaaaatc atttcattca tgtggtgtat atggtatggg
    34141 cgtcgttaca agtattgttg ctcaaaatac attgggcgta caacatattc ataatttaaa
    34201 tcatcaatgg gtagatgaac aacttgatag tgtcttcaat gataccttac ctcatgctat
    34261 taaaacgggg atgattgcta cagcagatac tatggaaacg attcgtcatt atttaatgca
    34321 acatgaatct attccatatg taattgatcc tgttatgttg gcgaaaagcg gtgattcatt
    34381 aatggataat gacacaaagc aaaacttgca gcatacgtta ttgccattag ctgacgtagt
    34441 aacaccgaat ttaccagaag ctgaagaaat aacgggacta accattgata gtgaagaaaa
    34501 aattatgcag gctggccgca tctttattaa tgagattggt agtaaaggtg tcatcattaa
    34561 aggcggtcat tcaaatgata ctgatatagc aaaagattat ttatttacta acgaaggtgt
    34621 tcaaacattt gaaaatgaac gatttaaaac aaaacatacg catggaacag ggtgtacatt
    34681 ttcagcagtt ataacggcag aacttgcaaa aggtagacca ttatttgagg ctgtacacaa
    34741 ggctaaaaag tttatttcaa tgagtataca atatacgcct gaaatcggcc gtggtagagg
    34801 tccagtgaat cattttgcat atttaaagaa agagggatta gacgatgaat tatctaaata
    34861 aaatacgtat tgaaaaccca ttaacaattt gttatacaaa cgatgtagtt aaaaatttta
    34921 cagcgaatgg tttattaagt attggtgcta gccctgcaat gagtgaagct cccgaagaag
    34981 ctgaagaatt ttacaaagtt gcacaagcgc tattaatcaa tatcggtact ttaacagcag
    35041 aaaatgaaca agatattatt gcgattgctc aaacggcaaa tgaggcaggc ttacctattg
    35101 tatttgaccc tgtagctgtt ggtgcttcta catatcgaaa gcaattttgt aaattattat
    35161 tgaaatcagc gaaagtatca gtaattaaag gcaatgcatc tgaaatatta gcgttgattg
    35221 atgatacagc aactatgaaa ggtacagata gtgatgctaa tcttgatgcg gttgcaatag
    35281 cgaaaaaggc ttacgcaaca tataaaactg caatagtaat cacaggtaaa gaggacgtta
    35341 ttgttcaaga taataaagcc ttcgtattag ctaatggatc tccattatta gcacgagtaa
    35401 ctggagctgg ttgtttatta ggaggcgtta ttgctggatt tttatttaga gaaacagaac
    35461 cagacataga agcgttaatt gaagcggtaa gcgtatttaa tattgctgct gaggtagctg
    35521 ctgaaaatga aaattgtggt ggtcctggta cgttttcacc attgttgctt gatacgttat
    35581 atcatttaaa tgaaacaacc tatcaacaac ggattcgtat tcaagaggtg gaataatatg
    35641 tttaaccaat cgtatctaaa tgtgtatttt atttgtggga cctccgatgt tccgagtcat
    35701 cgaactattc atgaagtatt agaagcagca ttaaaggcag ggattacact tttccaattt
    35761 cgagaaaagg gtgaatcagc tttaaaagga aacgataaac ttgtattagc taaagaatta
    35821 cagcatctct gtcatcaata tgatgtgcct tttatcgtta atgatgatgt atccttggca
    35881 aaagaaatta acgcggatgg tatacatgtc ggtcaagatg atgcgaaagt caaagagata
    35941 gcacaatatt tcactgataa aattattgga cttagtatta gtgatttaga tgagtatgca
    36001 aaatctgatt taactcatgt tgattatata ggtgttggac caatctatcc tacgccgtcg
    36061 aagcatgacg cgcatatacc agtaggtcca gaaatgattg caacgtttaa agaaatgaat
    36121 ccgcaactac cgattgtagc tattggcggt atcaatacta ataatgtggc gccgattgta
    36181 gaagcaggtg ccaatggaat ttcagtcatt tcagccattt ctaaaagtga aaatattgaa
    36241 aagactgtta atcgattcaa agattttttt aataattagt taaatttttc cacttattgt
    36301 ttcatacgta ctcattaatg tgatagaata aatcctatgt gaatataatg atagaggtgg
    36361 aaaaatgaag aaaaaagcgt tactaccatt atttttaggt attatggtct ttttggctgg
    36421 ttgtgactat tctaaacctg aaaaacgtag tgggtttttc tacaatacat tcgtagatcc
    36481 aatgaaaaat gtattggatt ggttgggaaa taacttatta aacgacaatt atggtttagc
    36541 tattattatc cttgtattgg taattcgtat tattttatta ccattcatgt tgtcaaacta
    36601 taaaaatagt catatgatgc gtcaaaaaat gaaagttgca aagccagaag ttgaaaaaat
    36661 tcaagaaaaa gtgaaacgtg cgcgtacaca agaagaaaaa atggctgcaa accaagaatt
    36721 aatgcaagta tataaaaagt atgacatgaa cccgattaag agtatgttgg gttgtttacc
    36781 aatgctaatc caattaccaa tcatcatggg attatacttt gtacttaaag accaacttgt
    36841 agatggtttg tttaaatatc cacacttctt atggttcgat ttaggacgtc ctgatatttg
    36901 gattacaatt attgccggtg ttttatactt tatccaagca tatgtatcaa gtaaaacgat
    36961 gccagacgaa caacgtcaaa tgggttacat gatgatggtc atttcaccaa ttatgattat
    37021 ctggatttca ttaagctcag catcagcact tggtttgtac tggtcagtca gtgcggcgtt
    37081 ccttgtagtt caaacacact ttgcgaacat ttattatgaa aaagtcgcta aaaaagaagt
    37141 acaacctttc attgaagcgt atgaaagaga gcacaacggc ggcagcaata aaaaaggcaa
    37201 aaacacacaa gttgtgtcta aaaagaaaaa gaaataacct tagcaagtcc aaataatgtg
    37261 caccctaaaa gttgaagata attttaactt tatagggcgc acattatttt tcgcttcaat
    37321 agcgaaactt agtgtcacat tatacaatat gtgataaata gaaacagcag taagatgatt
    37381 tcaaatgtga aagtcatcct actgctgttt ttatttgaaa taatattaac gagttttcag
    37441 tatgtaaaaa gtggttaata acagagttgt cattcttcat cgcaatgtca tcttgacaca
    37501 atgattcatc agtgtagcag tatatgtcgc aaatttccac agcaattcac ttatacatgt
    37561 aacagtgtga aataataacg tcacaacggc ataatacaca ttgcaacgtc ccaacaacat
    37621 atcatagaat cgtaacggca tgacatcaac accaaacctg gctataacac cataacagcg
    37681 caacgccaca tcatagcact acatcaagtc gcatcgtagc accagcacat caaagtaaca
    37741 tggcatcaac atcaaaccgg tctataacac cataacaccg caatgccaca tcatagcact
    37801 acatcaagtc gcatcgtggt accagcacat caaagtaaca tggcatcaac gccaaacctg
    37861 tctataacac cataacaccg caatgccaca tcatagcact acatcaagac gcatcgtggt
    37921 accagcacat caaagtagca tgacatgtca ccaagctccc acaacaacac atcaaagtaa
    37981 catggcatga caccaagctt ccacaacaac acgccattac atcgcatcat catactacaa
    38041 cttcaatgtc tagtcgtgac aattccattc cgtaaaaaac tgtttcaagt acatcatcat
    38101 aaagtcgtga cgttctttag caatcatctt cgccgtatct gtatgcatta aagattctaa
    38161 cttaagtaat ttttcaaaga aatgtttaat tgcagatggt ggcaactgtt caactaaatc
    38221 atcattaatc ttatctagtg acatatgttc tgtccacata ggttcaccaa agtgtcctgc
    38281 aaattgaaat gttcgtgcaa cacctatagc gcctatagca tcaagacgat ctgcatccct
    38341 gacaatttga ccttctaaag ataaagtgac atgatcattt ttgccattgc gatagctcat
    38401 attattaata ataaataaaa tgtgctcttg atcttcggtt gatagtgata aagaagataa
    38461 aaatgacttc aattcaacat attgtttgtt agcatctaca actttttcgt caacggtatc
    38521 atgaagcaaa catgcgagtt caatgactaa agtatcatta acaccttcat tttcagcgat
    38581 tgatttagct aaagcagtga cacgatatac atgcgcaata tcatggcctg tataatcatt
    38641 ttgatgaata gattccatat attttttggc agcactaatg acgtctgatt gttgcattaa
    38701 agttacactc ctcatatttc tattttataa gataggtgac aataattgtg aaataccttc
    38761 tttaaattta atccaaagac ttcgcttagc ataacgtgct tttgttaatt cagaagatac
    38821 tgctaaatca tctataaaag cttgttttaa ttttttggca atttgttggt cataaataaa
    38881 agcgttgact tcgaaattca atgtgaaact gcgatggtcc atattagctg ttcccacact
    38941 tgcaatttca tcatctataa caagtgtttt tgagtgtaaa aagccattgt cgtagtgaaa
    39001 tactttaaca ccggcatcta ataaggatgc tgcatttttt aaagtagccc aaaaaacaaa
    39061 cggatggtca ggtttattag gaatcatgat attgacatca acaccaccta atgccgcaat
    39121 tttaatagaa tctaaaaagg cttgatcagg tatgaaatag ggagattgaa tataaatcga
    39181 ttttttagca gatgaaatca ttttcaaata gccgtattta atctgttccc attcttcgtc
    39241 aggaccacta gaagctattt gaacgccaat tgttccacca gaatttacat ctgggaaata
    39301 acgatcatca taggagatgt ggtcacgtgt ggcttgtgaa ttccaatcta gaataaatcg
    39361 taattgcaat gcattcactg catccccgac aattcgtaaa tgcgtatctc gccaatagcc
    39421 gaattttttt gatttaccta agtactcatc accaacatta aaaccaccaa catatccaat
    39481 ttgcccatct attacaacaa tttttcgatg gtttcgattg ttcatacgca agttaattaa
    39541 aggtaatttt gatgggaaaa atgcttcagc atgtccacct ttattgcgaa acgggcgtaa
    39601 gcctttttta cgcagtccac gagaacccat gtcatcataa agaattttaa cttctacacc
    39661 ttgttccgct tttttaccaa gttcatttaa aatggtacga cctaattcat cattttgaat
    39721 aatatagtac tgaaaatgaa tataatcagt agcattacgg atgtcttgta ttaggtcatc
    39781 aaatttttct tggccgtctg tgtatatttt taaatcgttg tctgttgtta aaaatgctgc
    39841 gttattatat aacaacattt gaatcatttc tttaaatttt acaatttgat aattggaatt
    39901 tgaaaagttt tcatttttta aagcagctaa ttgctcatca acgattaact ctaatccttt
    39961 tttatcttcc ttatcaattt tgaaaatttg gtcacgttga atttgtcgtc ctaatagtaa
    40021 gtataaaatg aagccgaata aaggcaagaa aactaagact agtaaccaag cccagataga
    40081 attggcagaa cgtctttcca tgaaaataat ggtaaaggcg aataataagt ttaaaataaa
    40141 tgcaccaata aatattgaat ttaaaataat attagaatgc ttgagtgcaa tggataataa
    40201 ctctatcatt tgaaacctcc catcgaaaat cttaaacgag tagtatatct tattaattgt
    40261 atcatgaagc aatttaaata tcatgacaat ttagtttgaa aaacacactt tgtgttgacg
    40321 ttgtgtttta tgggatatag tatagtaagg aatgtaaatg aaggagtgaa tgctatgact
    40381 gaacaagata atgcacatca ttctgaacaa ataaaaacga atcttaaatc acgtttaaat
    40441 cgaattgaag gacaagtgag agcgattaat cgcatgattg aagaagatgt ctattgtgat
    40501 gatgtcctta cgcaaataag agcgacacgt tcggcgttaa acagtgttgc gataaagtta
    40561 ttagaacaac atatgaaaag ttgtattatg aataaagtta atcaaggtgc tcaggaagag
    40621 gcaatggaag agttattagt gacttttcaa aaattgatta aagactaaag gagtttaaga
    40681 tgatacatca aaatacgatt tacacagcgg gaattgaaac agaagaacaa gtaagtcaat
    40741 tgacagaacg catttcaaat atgataggtg ttcatcaagt gaatattaat ataatagatg
    40801 gtcaagtaac tgtatcgtat gagacaccag caaatttgaa tagtattgaa aaagaaatct
    40861 atgatgaagg atacaaaatt gtattttagg gtataatgta gagtgcgcta tagattttaa
    40921 ttttgaaaat aaattaaaaa ttttgtaaat gatgtagtaa aggtatgtcg aatagggtaa
    40981 gataatattg aaagcgatcc aaacgaggtg ataataatga aacgtcctga aaagattcaa
    41041 aatgtagtca aactattgtc atcattaggt gtgaatatta aaaaaactaa atctcgttta
    41101 gacattatta atactttgcc agcatctaat aaagtaagtc acgaattaaa ataattgact
    41161 gtgattgtga atcacacacc gttaaagcta tcgtcggact attcgatgat agcttttttg
    41221 actggattta ctaaaatgaa agcaagtact atataacaaa atccctcttt agagtgtaca
    41281 ttttacaatg tgtacttcaa agagggattg ttattatcga gtaatatcta tatatgacat
    41341 tttaaatcga cgacgtaaat agactatata acgtcatctt gaaaagttat gatatttgaa
    41401 actcaaaata gtttaaatta attacttttt ggatggtata aatcgacata tcgttttggt
    41461 tcatgataat aaattgataa gactatacct attccagtca tcatactcca tagcgcacta
    41521 ccaccataac taataaatgg taatggaata ccagtgattg gtaacaactg aattgtcata
    41581 ccaatatttt gtaaaatatg gaacacaagt aaagtgacga aaccaacgat aaagattttg
    41641 ttaaattgat cttcaatttt cgcagctaat cttattagat ggaagattaa aaataaaaat
    41701 attaagatca atatgacaga accgataaag ccaagttcct ctccaatcac tgaaaagata
    41761 aagtcagtat gattttcagg tatataaact tcaccgtgat tgtatccttt acctagtaac
    41821 tgtccagaac cgatagcttt aagtgattca gttaaatgat agccatcacc actactatat
    41881 gtataggggt caagccatga attgattcgt cccatttgat acagttggac acctaataaa
    41941 ttttcaatta atgcgggtgc atatagaata cctaaaatga ctgtcattgc accaacaata
    42001 cctgtaataa agataggtgc taagatacgc catgttatac cacttactaa catcacacct
    42061 gcaataatag cagctaatac taatgtagtt cctaggtcat tttgcagtaa tattaaaata
    42121 cttggtacta acgagacacc aataattttg aaaaataata acaaatcact ttggaatgat
    42181 ttattgaatg tgaattgatt atgtctagaa acgacacgcg ctaatgctaa aattaaaata
    42241 attttcatga attcagatgg ctgaatactg atagggccaa acgtgtacca acttttggca
    42301 ccattgataa taggtgtaat aggtgactca ggaataacga gcaagcctat taataataga
    42361 cagattaaga aatacaataa atatgtataa tgtttaatct ttttaggtga aataaacatg
    42421 atgatacctg caaaaattgc acctaaaatg taataaaaaa tttgtctgat accgaaatta
    42481 gcactgtatt gaccaccgcc cattgccgag ttaataagca gaacactgaa aattgctaaa
    42541 acagctatag tggctactaa tacccagtct actttgcgaa gccaatgctt atccggctgt
    42601 tgacgagatg aataattcat tgcaaactcc ttttatactc actaatgttt atatcaattt
    42661 tacatgactt tttaaaaatt agctagaata tcacagtgat atcagctata gatttcaatt
    42721 tgaattagga ataaaataga agggaatatt gttctgatta taaatgaatc aacatagata
    42781 cagacacata agtcctcgtt tttaaaatgc aaaatagcat taaaatgtga tactattaag
    42841 attcaaagat gcgaataaat caattaacaa taggactaaa tcaatattaa tttatattaa
    42901 ggtagcaaac cctgatatat cattggagga aaacgaaatg acaaaagaaa atatttgtat
    42961 cgtttttgga gggaaaagtg cagaacacga agtatcgatt ctgacagcac aaaatgtatt
    43021 aaatgcaata gataaagaca aatatcatgt tgatatcatt tatattacca atgatggtga
    43081 ttggagaaag caaaataata ttacagctga aattaaatct actgatgagc ttcatttaga
    43141 aaatggagag gcgcttgaga tttcacagct attgaaagaa agtagttcag gacaaccata
    43201 cgatgcagta ttcccattat tacatggtcc taatggtgaa gatggcacga ttcaagggct
    43261 ttttgaagtt ttggatgtac catatgtagg aaatggtgta ttgtcagctg caagttctat
    43321 ggacaaactt gtaatgaaac aattatttga acatcgaggg ttaccacagt taccttatat
    43381 tagtttctta cgttctgaat atgaaaaata tgaacataac attttaaaat tagtaaatga
    43441 taaattaaat tacccagtct ttgttaaacc tgctaactta gggtcaagtg taggtatcag
    43501 taaatgtaat aatgaagcgg aacttaaaga aggtattaaa gaagcattcc aatttgaccg
    43561 taagcttgtt atagaacaag gcgttaacgc acgtgaaatt gaagtagcag ttttaggaaa
    43621 tgactatcct gaagcgacat ggccaggtga agtcgtaaaa gatgtcgcgt tttacgatta
    43681 caaatcaaaa tataaagatg gtaaggttca attacaaatt ccagctgact tagacgaaga
    43741 tgttcaatta acgcttagaa atatggcatt agaggcattc aaagcgacag attgttctgg
    43801 tttagtccgt gctgatttct ttgtaacaga agacaaccaa atatatatta atgaaacaaa
    43861 tgcaatgcct ggatttacgg ctttcagtat gtatccaaag ttatgggaaa atatgggctt
    43921 atcttatcca gaattgatta caaaacttat cgagcttgct aaagaacgtc accaggataa
    43981 acagaaaaat aaatacaaaa ttgactaact gaggttgtta ttatgattaa tgttacatta
    44041 aagcaaattc aatcatggat tccttgtgaa attgaagatc aatttttaaa tcaagagata
    44101 aatggagtca caattgattc acgagcaatt tctaaaaata tgttatttat accatttaaa
    44161 ggtgaaaatg ttgacggtca tcgctttgtc tctaaagcat tacaagatgg tgctggggct
    44221 gctttttatc aaagagggac acctatagat gaaaatgtaa gcgggcctat tatatgggtt
    44281 gaagacacat taacggcatt acaacaattg gcacaagctt acttgagaca tgtaaaccct
    44341 aaagtaattg ccgtcacagg gtctaatggt aaaacaacga ctaaagatat gattgaaagt
    44401 gtattgcata ccgaatttaa agttaagaaa acgcaaggta attacaataa tgaaattggt
    44461 ttacctttaa ctattttgga attagataat gatactgaaa tatcaatatt ggagatgggg
    44521 atgtcaggtt tccatgaaat tgaatttctg tcaaacctcg ctcaaccaga tattgcagtt
    44581 ataactaata ttggtgagtc acatatgcaa gatttaggtt cgcgcgaggg gattgctaaa
    44641 gctaaatctg aaattacaat aggtctaaaa gataatggta cgtttatata tgatggcgat
    44701 gaaccattat tgaaaccaca tgttaaagaa gttgaaaatg caaaatgtat tagtattggt
    44761 gttgctactg ataatgcatt agtttgttct gttgatgata gagatactac aggtatttca
    44821 tttacgatta ataataaaga acattacgat ctgccaatat taggaaagca taatatgaaa
    44881 aatgcgacga ttgccattgc ggttggtcat gaattaggtt tgatatataa cacaatctat
    44941 caaaatttaa aaaatgtcag cttaactggt atgcgtatgg aacaacatac attagaaaat
    45001 gatattactg tgataaatga tgcctataat gcaagtccta caagtatgag agcagctatt
    45061 gatacactga gtactttgac agggcgtcgc attctaattt taggagatgt tttagaatta
    45121 ggtgaaaata gcaaagaaat gcatatcggt gtaggtaatt atttagaaga aaagcatata
    45181 gatgtgttgt atacgtttgg taatgaagcg aagtatattt atgattcggg ccagcaacat
    45241 gtcgaaaaag cacaacactt caattctaaa gacgatatga tagaagtttt aataaacgat
    45301 ttaaaagcgc atgaccgtgt attagttaaa ggatcacgtg gtatgaaatt agaagaagtg
    45361 gtaaatgctt taatttcata gagattagtc gagggacctt ttacttataa aaatgatttg
    45421 aattaatact aaaagattac aaagaagagg tggttttgtg tgtaaataca aaattgcctt
    45481 tttcttttta tgttaaatct ataaatttga aactaaatca aggttaattc tatgtacaca
    45541 ctttatatag gaagtagttt gaatgtttat ataatgtttt acaaaaagat gtagtattat
    45601 aatgtctaat ttcacatgtg tttcagtaaa atttgttgtg gaatgttaac gatatacgta
    45661 ttttataaaa aattttttat aatgattatt cgaatgatgc gtaacgctta catcttatct
    45721 aatgctagct ttttgacaaa aatatgacaa tcaattaatg tgattctaat aaatattcgc
    45781 aaattgcttt attgcgatta aatttttttg gtggtactat atagaagttg atgaaatatt
    45841 aatgaactta tatgcaaaag tatattgaga aataaacagg taaaaaggag aattattttg
    45901 caaaatttta aagaactagg gatttcggat aatacggttc agtcacttga atcaatggga
    45961 tttaaagagc cgacacctat ccaaaaagac agtatccctt atgcgttaca aggaattgat
    46021 atccttgggc aagctcaaac cggtacaggt aaaacaggag cattcggtat tcctttaatt
    46081 gagaaagtag tagggaaaca aggggttcaa tcgttgattt tagcacctac aagagaattg
    46141 gcaatgcagg tagctgaaca attaagagaa tttagccgtg gacaaggtgt ccaagttgtt
    46201 actgtattcg gtggtatgcc tatcgaacgc caaattaaag ccttgaaaaa aggcccacaa
    46261 atcgtagtcg gaacacctgg gcgtgttatc gaccatttaa atcgtcgcac attaaaaacg
    46321 gacggaattc atactttgat tttagatgaa gctgatgaaa tgatgaatat gggattcatc
    46381 gatgatatga gatttattat ggataaaatt ccagcagtac aacgtcaaac aatgttgttc
    46441 tcagctacaa tgcctaaagc aatccaagct ttagtacaac aatttatgaa atcaccaaaa
    46501 atcattaaga caatgaataa tgaaatgtct gatccacaaa tcgaagaatt ctatacaatt
    46561 gttaaagaat tagagaaatt tgatacattt acaaatttcc tagatgttca tcaacctgaa
    46621 ttagcaatcg tattcggacg tacaaaacgt cgtgttgatg aattaacaag tgctttgatt
    46681 tctaaaggat ataaagctga aggtttacat ggtgatatta cacaagcgaa acgtttagaa
    46741 gtattaaaga aatttaaaaa tgaccaaatt aatattttag tcgctactga tgtagcagca
    46801 agaggactag atatttctgg tgtgagtcat gtttataact ttgatatacc tcaagatact
    46861 gaaagctata cacaccgtat tggtcgtacg ggtcgtgctg gtaaagaagg tatcgctgta
    46921 acgtttgtta atccaatcga aatggattat atcagacaaa ttgaagatgc aaacggtaga
    46981 aaaatgagtg cacttcgtcc accacatcgt aaagaagtac ttcaagcacg tgaagatgac
    47041 atcaaagaaa aagttgaaaa ctggatgtct aaagagtcag aatcacgctt gaaacgcatt
    47101 tctacagagt tgttaaatga atataacgat gttgatttag ttgctgcact tttacaagag
    47161 ttagtagaag caaacgatga agttgaagtt caattaactt ttgaaaaacc attatctcgc
    47221 aaaggccgta acggtaaacc aagtggttct cgtaacagaa atagtaagcg tggtaatcct
    47281 aaatttgaca gtaagagtaa acgttcaaaa ggatactcaa gtaagaagaa aagtacaaaa
    47341 aaattcgacc gtaaagagaa gagcagcggt ggaagcagac ctatgaaagg tcgcacattt
    47401 gctgaccatc aaaaataatt tatagattaa gagcttaaag atgtaatgtc ttgagctctt
    47461 ttttgttttc aataattgat tctctgtaga tatcaaagta ctaacgtttt aaaggttaaa
    47521 tatttaattg gattgagatc tgtatgcggt tatatcattc tgtgtaaata tggttctcca
    47581 ccaaatgtgg tgagtatata atttaaagaa ctatttttaa attaagaata atcgaacata
    47641 aataaacttt atgaaatttc agtatcatgt tcttataaaa aacaataggg ctttttgctg
    47701 acgctagtgc gcgataaata ataagttgaa tataaaaaag atcactgcca atcattcgtt
    47761 taatggcagc gatctttttt atttaattat ttctctttcc actgcaacat ttgataacca
    47821 atgcgtggat gtgttttaat aatatctttt gcgtcctcat gacattgtga aagtttttgt
    47881 cttaatgatg tcatatgcac tcttaaacta ggcatttcag tcttattaac atagccatat
    47941 attcgtttta atatcatctc ataagtgagt acttttcctt tatgatttga caatagttct
    48001 aacaagctaa attcatttgg cgtcaaatgt acctcctgat tattaataac aacagatttg
    48061 gagccaaagt cgatgcttag caaaccgtta gtaaatacaa tgttagtttc ttgatgtgac
    48121 ttagcgattc tctcgatgac tcgtattcgt gcccgaagct catcaacatt aaaaggttta
    48181 gtcatatagt cattcgcacc gttatctaaa gcttgaataa ttgtttgttc ttcttgtctt
    48241 gcacttatta caatgatagg aatgtcagta tgttgcctga tttctgaaat caaacataat
    48301 ccatctttat ctggtaaacc taaatctaat aaaatgacat ctggtttatc aatttgaatt
    48361 ttaaagtgtg cttgtgtggc attgtcggct gtagttacat tgtaataatc taaagttaat
    48421 gcaacatcaa gtaaatgtgt gattgcgtga tcatcttcaa ttatcaatat tttagattgc
    48481 attatacgtc tccttcgtta aagtctgtat atatattgaa atagaatata ctgccgtgtg
    48541 gttggttcgg tttatattgt aagtttgatt gatgtttgtg taggatagtc tgtactaaat
    48601 ataagcctag tcccatgctt tctttttggt tatctttaaa atatttattt gatcctgtgt
    48661 aaaaaggctc gaatatcttt tgttgttctt ctaaactaat tccaggtcct tcgtctataa
    48721 cggcaaattc gatttgttca tagctagcat aacgaataga taaattgatt ttggtgtcag
    48781 tagaagtgtg tttaactgca ttttcaatca aattgaataa agcttgtaaa atcaacttac
    48841 tgtcaatgtg tataaactgt aaatttactg aggatgatac agttatacgc ttttttaaat
    48901 ggcgacgttc taaaatcata tcgatttctt ctactaattc actaacgaga taaggttgca
    48961 attttatctg aacatttgat gactgtaatt ttgttaatga taaaatattt gtcactaata
    49021 gatataaata ctgactttct tgaaaactat gtacaagtaa ttgttccttt tctatgatag
    49081 acatatcttt actatgtgat actaaaatat ctaaatttcc cataattgtt gttaacggtg
    49141 tacgtatgtc atgcgaaatt gatcttaaaa aatttgaatg tgtcagttga cgttcagcct
    49201 gtaacatgga ttctctcgtt tgtttaagta acgtcacatt ttcaacggcg agagaaagtt
    49261 catttaacat tgattctaat attgatgcat catatggatt aatcacttga gaactttggt
    49321 aatcaatggc tagaatgcct ttaatcggag atgtgccaat tggtatcaac catttattaa
    49381 tgcctggaaa tgtatctgtt gttgcaccag cttgtctttc atttttaatt acccagctta
    49441 atgcttgttc atgctgttga gtcgtattat cgatatggtt ttgcaatggt attgttttaa
    49501 ttactttcga ttgattgata acgtatatag taattgattg ttgcaataat tgattaattt
    49561 ggtatccagc atttattagt aagttttcaa ctgtataagt ttgtttaatc gaatcattaa
    49621 attgaaataa taaatctgta cgataaagtt gctttttagt aatggagtat tggaatttaa
    49681 tttgttttaa taaagcactc gttaaaatac ttgttaaaat gctaacgata aatgtaatag
    49741 gatagtcaaa gcgatatact tcaaatgtat atctaggttc cgtaaaaaaa taattaaaaa
    49801 caaatacgtt aataattgct gctaaaaagc caatgatgaa ggaccgcgtc caaatggata
    49861 ataaaatgat gccgatgaga aaaatcatta aaataatcgt actagactca tgcttatcaa
    49921 gttgataaat ccacagtccc atcattacac aaattatttg aatcaaaatc atttttaaca
    49981 tatctatggc gaaacgtttg cctttaggac gatagggtga tttgttcatt ttcaattcaa
    50041 caggtatttg tttgattgga acgatttcga ttttatagct atgttcaaag gacattaaat
    50101 ggtcaattaa aggtgtattg aaaaagtctc gccacttatt tctaatatgt tgtccgataa
    50161 ttaatttggt tacatcttga tttttacacc attcgtctaa tcctaatgca acggtttggc
    50221 tataaactac ttttactttt gctcctaatg attttgcaag catgagatgt tgatgcactt
    50281 gcttttgact atctttatat tgcctgtttt tttcgaatac atctatataa atagcagtga
    50341 acttcgcatg ttctttttga gcaatatgga atgcctcttt aattactgct tcattataaa
    50401 tgctcccact aattgccaca gcaatatgag gtttgagtga cgttttatgg ttgtgtcgga
    50461 ctttttcttt atcactcatc aagtcggcaa ctgttcttaa cgtcaatgta cgcagttcgc
    50521 ttaggtgggc atacgtaaag aaattactaa atgctacatc tagcctatcc tttttatata
    50581 ccttgccagc ttttaagcgt ttaattaatt gttcaggtga gatatctacg acttctaata
    50641 catcggcgct cattatgaaa tagtcgggta cacgttcttt aacatgtaca ccggtcatca
    50701 gttcaatttg actacttaaa ctttcaatat gttgaatgtt caaagtggta tgaacatcga
    50761 taccatgatt taaaatttct tcaatatcca tatatcgttt ctcatgacga tctctagaaa
    50821 tattcgtatg tgctaactca tcgataagta ctattgtcgg cgattcttcg attatgcggt
    50881 cgacatctaa atattgaaaa tgatgactac catgtttagt aaaattggtt gtgattttag
    50941 gtaattgttc agctaatgca tttgtttcat cacgctgatg cggttcaata tatcctattt
    51001 taatatcaac gttactttga aatagttcaa tggcatttga aagcatctca aatgttttac
    51061 caacacctgg actgtaaccg atgtaaattg ttaaagatcc acgctttttt cctatgttta
    51121 gcgattcagt gtttgacata accttcacct cgatagcaat atcataatat tcttcacaat
    51181 taaatgctat aaaatttatt tctgcataca catcttaatg atttcttaat aactaagttt
    51241 gaaaattaat atttctctca taaacaagtt gttacgatgt tgttgtgttg taaggaggtt
    51301 atggcaatgc tcatcgtttt attactggta gttattgcat tagttttgta tttattttat
    51361 gccttaattc gtagtgaaaa attttagaaa tatattaagt gagggttatc atggaaataa
    51421 ttttgtttct aacaatgatg gttatgatta cgtatgtttt tagtggatat ctatatcgcg
    51481 tagcattggt gcaatcgtca agagtagatt taatatttac tagatttgaa aatatgtgtt
    51541 ttaaaattat cggcacagat ttagaacaca tgtcagctaa aacatatgtc aaacattttc
    51601 tggcatttaa tggatttatg gggtttataa catttgtatt gctaatagtt cagcaatggc
    51661 tttttttaaa tccaaatcat aatttaaacc aatcgataga tttagcgttt aatacagcaa
    51721 tatctttttt aacaaatagt aatttacaac actataacgg tgaatcagat gtgacatatt
    51781 taacgcaaat gattgtaatg acatatttaa tgtttacatc tagtgcatca ggttacgccg
    51841 tttgtatagc gatgttgaga cgtttaactg gattaactaa tatcattggt aatttttatc
    51901 aagatattgt tcggtttatt gtccgagtac ttttaccatt atcatgttta attagtattt
    51961 tattgatgac tcaaggtgta ccacaaacgt tgcatgctaa tttaatgatt cggactttaa
    52021 gcggacatat tcaacatatt gcatttggac ctattgcatc acttgaatca ataaaacatc
    52081 ttggtacgaa tggtggagga tttttagcag gaaattctgc gacacctttt gaaaatccaa
    52141 atatttggag caattttata gaaatgggca gtatgatgtt acttcctatg tcaatgttgt
    52201 tcttatttgg tcgcatgtta agtagacatg gtaaacgagt acatcgtcat gcgttgatat
    52261 tatttgtcgc aatgtttttc atttttatag caattcttac attaactatg tggagtgagt
    52321 atcgtggtaa tccaatacta gcgaatttag gcatttatgg accgaatatg gaaggtaaag
    52381 aggtacggtt tggagcaggt ttgtcagcac tatttacagt tattacgacg gcatttacaa
    52441 cgggttctgt taataacatg catgatagct taacgcctat aggtggatta ggaccaatgg
    52501 tattaatgat gctaaatgtt gtatttggtg gcgaaggcgt aggactcatg aatttattga
    52561 tatttgtctt actgacggtg tttatatgca gtttgatggt tggtaaaaca ccagaatatt
    52621 taaatatgcc aattggcgcc cgtgaaatga aatgtattgt cttagtcttt ctcatacacc
    52681 caattttaat tttagtattt tcagcacttg cttttatgat tcctggagca agtgaaagta
    52741 taacgaatcc gtcttttcat ggtatttcac aagttatgta tgaaatgaca tcagctgctg
    52801 cgaacaatgg atcagggttt gaaggactga aagatgatac aacattctgg aatatctcta
    52861 caggaatcat tatgttgctt tctcgttata taccaattat tttgcaatta atgattgcat
    52921 caagtttagt gaataaaaaa tcataccatc aagataaata tacaatagcg attgataaac
    52981 cttattttgg cgtatcacta atcgtattta tcgttttact aagtggtttg acatttattc
    53041 cagtactatt acttggtcct attggtgaat ttttaacttt aaaataaagt aaagggatgg
    53101 aaaaaatgca tcatgtaaat aaatatttca atcaaacaat ggtgatagaa gctttgaaaa
    53161 tgagttttta taagctgaat cttaaacaat taataaaaaa tccgataatg tttgtcgttg
    53221 aggttggaat gattttaacg ttgatattaa tatgttttcc tgacatattt ggaactagct
    53281 atctatcgcg aggttatttg attacaattt ttatcatatt attaataact atattgtttg
    53341 ctaatttttc agaagccttt gctgaaggtc gtggaaaagc gcaagctgat agtttaagac
    53401 aagcacaatc aaatttaact gctcgattga tagaagaaaa tggtgcgtat cggatagtga
    53461 acgccacaga gttaaaagcc ggacaaaata ttcgggtaga aaatggagaa acaatccctg
    53521 ctgatggcgt tgtaataaat ggattagcaa ccgttgatga atctgcaatt actggtgagt
    53581 ctgcacctgt gataaaagaa tcaggtggcg attttgatgg agtcattggc ggtacacttg
    53641 taacatctga ttggttagaa atacgtgttg agagtgaagc gggtacatct tttttagata
    53701 aaatgattgc cttagttgaa ggggcggaaa gaaataaaac accaaatgaa attgcacttt
    53761 ttacattatt aacaacgtta acaattatat ttttagtcgt tattgtaacg ttatatccaa
    53821 tagcatctta cctgcattta attttaccta ttgcgatgct cattgcgtta acagtatgtt
    53881 taataccaac gacaatcggt ggtttgttat cggcgattgg tattgcaggc atggatcgtg
    53941 ttactcaatt caatgtatta gcgaaaagtg ggcgtgctgt agaagtatgt ggagatgtcg
    54001 atgtaatgat tttagacaag acaggcacaa tcacttatgg caatcgaatc gctagtgaat
    54061 ttttaccagt gaatcaacaa atgcttgaaa aattgatagt cgctgcttat atgtcatcaa
    54121 tctacgatga tacgccagaa ggaaaaagta ttgtacgatt agcgaagcag atgtatataa
    54181 atgagctgcc taaagacatt gatggcacat ataagccttt tacagctgaa actagaatga
    54241 gcggaatcat tacaaatgaa atatcggtat tcaaaggtgc acctaatagt atgataaatc
    54301 ttgtcaaaca acaacaaggc aatattcctc ttaatattga gtctctctgt atggatgtat
    54361 cgagtaaagg tggcacacca ttaatagtca ttgaaaacaa tgtcatgctt ggcgtgattt
    54421 atttaaaaga tgtgattaaa gatggtcttg tagaacgatt tactgaattg agaaaaatgg
    54481 ggattgaaac agttatgtgt acaggtgata atgctttaac agcagctaca attgcaaaag
    54541 aagccggggt tgatagattt gttgcagagt gtaaacccga agataaaatt aaagtcatca
    54601 aagatgaaca agcgaaaggc catattgttg cgatgacagg agatggtaca aatgatgcac
    54661 ctgcgctagc acaagcgaat ataggattag ctatgaactc agggacaatt agtgctaaag
    54721 aggcagctaa tctaattgat ttagattcga atcctacaaa gttaatagag gttgtaaaaa
    54781 taggcaaaca attattaatg acgagaggtg cattaacgac atttagtttg gctaatgacg
    54841 tagcaaaata ctttgcaatt ttacctgcac taatgatgtc aacaattcca gaaatgactt
    54901 cactaaatat tatgcattta tcgtcaccta aatctgcaat tatatcagct ttgatattca
    54961 atgcattaat cattgttgcc ttaattccaa ttgccatgaa aggtgtaaaa gtaaagggtt
    55021 acagcattga tagaatcttt ataaataata tgttgattta tggattaggt ggattaatcg
    55081 tcccgttttt aggcattaaa ttaatagata tgattgtaca attttttgtg taaagaggaa
    55141 ggagcctgta ccatgaatac gataagaaat agtatttgtt taacgataat aactatggta
    55201 ttatgtggat ttttattccc gttagctatc acgttaattg gtcaaatatt tttttatcaa
    55261 caagcaaatg gcagtttaat tacgtatgac aatcgtatag ttggatcaaa attgattgga
    55321 cagcactgga cagagacacg atattttcac gggagaccga gtgcagttga ttataatatg
    55381 aatcctgaaa aattatataa aaacggcgta tcttcaggtg gaagtaatga atcgaatgga
    55441 aacaccgaat taatagcacg catgaaacat catgttaaat ttgggaattc aaatgtaaca
    55501 atagatgctg caacaagttc tgggtcgggc ttagatccgc atattacggt cgaaaatgca
    55561 ttgaagcaag cacctcgtat tgctgatgct cgtcatgtat ctacttcaag agtggccgac
    55621 ttgattcaac atcgtaagca gcgtggtgtg ttaacaaatg attatgtgaa tgtgcttgaa
    55681 ctaaatatcg ctcttgataa gatgaaagat tgattataga tgaaaggcat catgttgata
    55741 aaattataca tggtgtcttt ttagcaattc aagcactttc tgcttaatta aatttttaga
    55801 tgtaccttta ggaagaataa attagcatga aatattccat atgctataat tatcatcata
    55861 taaaaataaa atgaaataga ttataaatcc gtataatttt ttatttgggg agaggggtat
    55921 atgtcagatt atactaagtt gactaagagt cctaagtcag cattgttgta ttactacatt
    55981 acgaatggct tagaatttat attgagtgta gctgtgtatg tcatttttta ttttatatgg
    56041 ttaagatttg aatggccaca ttacttactt tatattttgt tggttttagg tgctctgagt
    56101 acattaaaat tatttattaa gcctttatgg caatatcatt gtcgctttta taaagttgat
    56161 caactaagtg tacaatatcg tacatcattt tttatatata aagaagaaac gagtcgaatt
    56221 gaacgtttac aatacttgtc gattaaatct aatccaatta gtaaagtgct taatttgtat
    56281 aaagttggat ttatgactgc aggtcatact atttatttac cgatgatgtc tcatgacgat
    56341 gttaaaataa tcgaagagag aactatgtcg aatttgagag gggttgaaag tgatgtctga
    56401 acctcaaaaa ttacatccga tttcatattt taatggcata atcaatgcta tcaaacaaaa
    56461 catagtcgtc ttttttattt tcattatctt tcaattgaaa gattttgatt atacgaatcc
    56521 cgaatcatat ttatggatag gtattgtatt tgtattcttt ttaatatcat acatttctca
    56581 aattgtcgga attatgaaca cgcgatattg gattgaggat aattatttca ttttaacaac
    56641 aggtattttt aataaaaagc gtaaagaatt aaatataaaa cgtattcagt ccgttgatat
    56701 gacacaaggg gtcgtcaatc aaattattgg tggtgtggat ttacaaatca aaacaccaag
    56761 tgatggtatt gtcttaagtg ttatttcgaa aaaacaaggt gaatatcttg aacgatatat
    56821 tgatcaattg caaacagagt tgaagacaga agtggcgcaa aagtctagtg ctgagaagaa
    56881 tgacaactgt aatcatgaca ttgaaggtaa tgtggaagac atcgcagaag gtcgcttaga
    56941 tgaagtgcag aacactgata gtatgcttaa tcagaaaaat aaacgcattc ctatatatca
    57001 gatgaatttt aaagaatcac tatttatggc aatgaccagt ggcgccattg gtgtaacgtt
    57061 agcagcttta gtaccgattt acggggctgt aagagagttg atcccatggt cgaagctaaa
    57121 tcatgaaatt agtcaatggg ttcaaattgt aagtatttca gtattaatca tcattttact
    57181 agtattaata gtggcatata ttattggaac gattataaca atgataagat tttatggctt
    57241 ttcggtgatg cttgaaaatg agcaacttaa aattgaatat ggtctcttta acaagaagaa
    57301 agtaactgta ccaacgaaac gattgcaagc tgttgttgaa cgtcagtcat atatacgaag
    57361 attatttggc tacacagcaa tacattttat catcacaagt gattttgaaa ataaaaaaat
    57421 cgaagatgat gacaatgatt caggtagtgt gccgatattg ccgtttatta aacgagacaa
    57481 agcatatcaa attatccaga atttagtacc agatttatct tatcaacaag ttaatgaagg
    57541 tatgcctttg tcaggtttcc acagacactt tttaatacct agtattattg tcatagctat
    57601 ggcaggtata ggctgttatt tttggaatca attattattg ttatttacga ttataacaat
    57661 agtcattatt ggtttatttg tgatcaaagg atatctttat gtcaaacatg cgggttttaa
    57721 aatgacagaa gaacaaatta ctatccagaa tttcgagcta ttgaaacgaa atatttatta
    57781 ttttaaagcg catcacatta taggatttga cacttggcaa catacattac ttaaacgcaa
    57841 caatttaaat aattttagct ttattgttgc taaaggtgtg ggagcccaat cgattaactt
    57901 aaaatacgca agaagtgaag atgcaaataa attgaaatca tggtatttaa gaggtgaaaa
    57961 tgatgaggga ttataatttt atgtcaccac acgctaaaaa agtgatgagg ttaagtgctg
    58021 tgctgttttg gatacccata gcgcttgtac ttttaatcgc atttaatatt ttgaactggc
    58081 tattttggga gattttagat aaccatttat ccattgttag tagtgtgatt ataatattac
    58141 ttgttgctgt tttcacgctt gttatagcgc cagtctatcg atataagcac tgcagatatg
    58201 catttgaaaa acaccatttg cgtgtacgaa acggcatttt atttttggat gaaaaggtaa
    58261 taccatattt tcgtattcaa aatattgata tcgatgttgg tcctataatg agacgctatc
    58321 gattagcaac tttgaccctt tatacagcgg gtggtcaagc tgaaattgaa ttaatagata
    58381 gaagtgaagc acgtagattg aaacgatggt taaataacga gagacattta gaaaacaata
    58441 aaaatgagga ataatcaatg atacatggaa ttggtgtaga tttaatcgaa atcgatcgaa
    58501 tacaagcgtt atatagtaag caaccaaaat tggttgagcg gattttaact aaaaatgaac
    58561 agcacaaatt caacaatttc acacatgagc aacgtaaaat tgaattttta gctggcaggt
    58621 ttgctacaaa agaagcgttc agtaaagcat taggcacagg cttaggaaaa catgtagctt
    58681 ttaacgatat agactgttac aacgacgaac ttggcaaacc aaagattgat tacgaagggt
    58741 ttatcgtaca tgttagtatc tcacacactg agcattatgc gatgagccaa gttgttttag
    58801 aaaagtcagc attttaaatt tgttatacaa tgataattaa gatctagttt taaaacgtgt
    58861 gggaattaca ggaggaagta atatgtcgga taaatattat agatctgcgt atatgaatgt
    58921 agatttaaac gctgttgcat caaatttcaa agtattcagt acattgcatc caaataaaac
    58981 agtgatggct gtcgttaaag ccaatgccta tggactaggt agtgttaaag tagcacgtca
    59041 tttaatggaa aatggcgcca cattttttgc tgtagcaacg ttagatgaag cgatagaact
    59101 tagaatgcat gggattactg ctaaaatttt agtcttaggt gtgttaccag ctaaagatat
    59161 tgataaagcg atacaacacc gagttgcctt aacggttccg tctaaacagt ggttgaaaga
    59221 agcaattaaa aacatttctg gtgagcaaga gaaaaagtta tggttgcaca ttaaattaga
    59281 tacaggaatg ggacgtttag gtattaaaga tactaaaacg tatcaagaag tgattgaaat
    59341 cattcaacaa tatgagcaac ttgtatttga aggcgtgttt acacactttg cctgtgctga
    59401 cgaaccagga gatatgacaa ctgaacaata tcaacgtttt aaagatatgg tcaatgaagc
    59461 aattaaacct gaatatatac attgtcagaa ctcagcaggc tctctattaa tggattgcca
    59521 attctgtaat gcaataagac caggaatttc cctttatgga tattatccat cagagtatgt
    59581 acagcaaaaa gttaaagtac accttaaacc aagtgtgcaa ttaattgcta atgtagttca
    59641 aacaaagacg ctacaagcgg gtgagtctgt aagttatggt gcaacttata cagctactga
    59701 cccaactaca atagcattgt tacctattgg atatgcagat ggctatttac gcataatgca
    59761 aggtagcttt gtaaatgtaa atggtcatca atgcgaagtt attggtcgcg tatgtatgga
    59821 tcagacaatt gttaaagtgc cagatcaagt taaagctgga gattcggtga ttttaataga
    59881 taatcataga gaaagtccac agtcggtaga ggtggtagct gaaaagcaac atactattaa
    59941 ttatgaagtg ctttgtaact tgtcgagacg tttgccgcga atctatcatg atggtgatca
    60001 acgttttgta acaaatgaat tgttaaaata atatggtcag tgcatataaa atttgttatc
    60061 attagagtaa ttaaaggtca tttaataact tttggaatca attggaggtt ctcatatgtt
    60121 atcttttagt caaaatagaa gtcatagctt agaacaatct ttaaaagaag gatattcaca
    60181 aatggctgat ttaaatctct ccctagcgaa cgaagctttt ccgatagagt gtgaagcatg
    60241 cgattgcaac gaaacatatt tatcttctaa ttcaacgaat gaatgattag acgaggagat
    60301 gtttatttag cagatttatc accagtacag ggatctgaac aagggggagt cagacctgta
    60361 gtcataattc aaaatgatac tggtaataaa tatagtccta cagttattgt tgcggcaata
    60421 actggtagga ttaataaagc gaaaataccg acacatgtag agattgaaaa gaaaaagtat
    60481 aagttggata aagactcagt tatattatta gaacaaattc gtacacttga taaaaaacga
    60541 ttgaaagaaa aactgacgta cttatccgat gataaaatga aagaagtaga taatgcacta
    60601 atgattagtt tagggctgaa tgcagtagct caccagaaaa attaggcgtc tattatatgt
    60661 atttttcaga gataaataaa atat
//
LOCUS       DANJFZ010000003        51634 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.003, whole genome shotgun sequence.
ACCESSION   DANJFZ010000003 DANJFZ010000000
VERSION     DANJFZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 51634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.003"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(408..506)
                     /locus_tag="PWN45_000160"
     CDS             complement(408..506)
                     /locus_tag="PWN45_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621964.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="PWN45_000161"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="PWN45_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL0621965.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="PWN45_000162"
     CDS             complement(1314..1631)
                     /locus_tag="PWN45_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL0621966.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PWN45_000163"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PWN45_000163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL0621967.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="PWN45_000164"
     CDS             complement(3487..4011)
                     /locus_tag="PWN45_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0621968.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="PWN45_000165"
     CDS             complement(4149..4250)
                     /locus_tag="PWN45_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621969.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="PWN45_000166"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWN45_000166"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PWN45_000167"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PWN45_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL0621970.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PWN45_000168"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PWN45_000168"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL0621971.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PWN45_000169"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PWN45_000169"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL0621972.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9005..9097)
                     /locus_tag="PWN45_000170"
     tRNA            complement(9005..9097)
                     /locus_tag="PWN45_000170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9059..9061),aa:Ser,seq:tga)
     gene            complement(9366..10829)
                     /locus_tag="PWN45_000171"
     CDS             complement(9366..10829)
                     /locus_tag="PWN45_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDL0621973.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="PWN45_000172"
     CDS             complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="PWN45_000172"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDL0621974.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15527..16411
                     /locus_tag="PWN45_000173"
     CDS             15527..16411
                     /locus_tag="PWN45_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0621975.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16526..17644
                     /locus_tag="PWN45_000174"
     CDS             16526..17644
                     /locus_tag="PWN45_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0621976.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17641..18423
                     /locus_tag="PWN45_000175"
     CDS             17641..18423
                     /locus_tag="PWN45_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0621977.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18592..19077)
                     /locus_tag="PWN45_000176"
     CDS             complement(18592..19077)
                     /locus_tag="PWN45_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0621978.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19067..19462)
                     /locus_tag="PWN45_000177"
     CDS             complement(19067..19462)
                     /locus_tag="PWN45_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL0621979.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19611..19880
                     /locus_tag="PWN45_000178"
     CDS             19611..19880
                     /locus_tag="PWN45_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621980.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="PWN45_000179"
     CDS             complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="PWN45_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDL0621981.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="PWN45_000180"
     CDS             complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="PWN45_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDL0621982.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22280..22939)
                     /locus_tag="PWN45_000181"
     CDS             complement(22280..22939)
                     /locus_tag="PWN45_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0621983.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22943..23968)
                     /locus_tag="PWN45_000182"
     CDS             complement(22943..23968)
                     /locus_tag="PWN45_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDL0621984.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24262..25404)
                     /locus_tag="PWN45_000183"
     CDS             complement(24262..25404)
                     /locus_tag="PWN45_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDL0621985.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25388..26302)
                     /locus_tag="PWN45_000184"
     CDS             complement(25388..26302)
                     /locus_tag="PWN45_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL0621986.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26519..27856)
                     /locus_tag="PWN45_000185"
     CDS             complement(26519..27856)
                     /locus_tag="PWN45_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL0621987.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28021..28134)
                     /locus_tag="PWN45_000186"
     CDS             complement(28021..28134)
                     /locus_tag="PWN45_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621988.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28312..29046)
                     /locus_tag="PWN45_000187"
     CDS             complement(28312..29046)
                     /locus_tag="PWN45_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDL0621989.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29083..29757)
                     /locus_tag="PWN45_000188"
     CDS             complement(29083..29757)
                     /locus_tag="PWN45_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0621990.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29795..30211
                     /locus_tag="PWN45_000189"
     CDS             29795..30211
                     /locus_tag="PWN45_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0621991.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30440..30802)
                     /locus_tag="PWN45_000190"
     CDS             complement(30440..30802)
                     /locus_tag="PWN45_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDL0621992.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30964..33669)
                     /locus_tag="PWN45_000191"
     CDS             complement(30964..33669)
                     /locus_tag="PWN45_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDL0621993.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33682..35166)
                     /locus_tag="PWN45_000192"
     CDS             complement(33682..35166)
                     /locus_tag="PWN45_000192"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDL0621994.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35687..35752
                     /locus_tag="PWN45_000193"
     CDS             35687..35752
                     /locus_tag="PWN45_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDL0621995.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35784..35849
                     /locus_tag="PWN45_000194"
     CDS             35784..35849
                     /locus_tag="PWN45_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDL0621996.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35901..35969
                     /locus_tag="PWN45_000195"
     CDS             35901..35969
                     /locus_tag="PWN45_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDL0621997.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36033..36095
                     /locus_tag="PWN45_000196"
     CDS             36033..36095
                     /locus_tag="PWN45_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDL0621998.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36754..37956)
                     /locus_tag="PWN45_000197"
     CDS             complement(36754..37956)
                     /locus_tag="PWN45_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0621999.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38075..38335
                     /locus_tag="PWN45_000198"
     CDS             38075..38335
                     /locus_tag="PWN45_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622000.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38625..38939)
                     /locus_tag="PWN45_000199"
     CDS             complement(38625..38939)
                     /locus_tag="PWN45_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622001.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38958..39281)
                     /locus_tag="PWN45_000200"
     CDS             complement(38958..39281)
                     /locus_tag="PWN45_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622002.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39278..40606)
                     /locus_tag="PWN45_000201"
     CDS             complement(39278..40606)
                     /locus_tag="PWN45_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622003.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40596..41408)
                     /gene="lpl10"
                     /locus_tag="PWN45_000202"
     CDS             complement(40596..41408)
                     /gene="lpl10"
                     /locus_tag="PWN45_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HDL0622004.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            complement(41591..42376)
                     /gene="lpl9"
                     /locus_tag="PWN45_000203"
     CDS             complement(41591..42376)
                     /gene="lpl9"
                     /locus_tag="PWN45_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HDL0622005.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            complement(42395..43207)
                     /gene="lpl8"
                     /locus_tag="PWN45_000204"
     CDS             complement(42395..43207)
                     /gene="lpl8"
                     /locus_tag="PWN45_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HDL0622006.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            complement(43386..44162)
                     /gene="lpl7"
                     /locus_tag="PWN45_000205"
     CDS             complement(43386..44162)
                     /gene="lpl7"
                     /locus_tag="PWN45_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HDL0622007.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
     gene            complement(44232..45023)
                     /gene="lpl6"
                     /locus_tag="PWN45_000206"
     CDS             complement(44232..45023)
                     /gene="lpl6"
                     /locus_tag="PWN45_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HDL0622008.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            complement(45055..45825)
                     /gene="lpl5"
                     /locus_tag="PWN45_000207"
     CDS             complement(45055..45825)
                     /gene="lpl5"
                     /locus_tag="PWN45_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDL0622009.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTEKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            complement(45844..46644)
                     /gene="lpl4"
                     /locus_tag="PWN45_000208"
     CDS             complement(45844..46644)
                     /gene="lpl4"
                     /locus_tag="PWN45_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HDL0622010.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            complement(46693..47500)
                     /locus_tag="PWN45_000209"
                     /pseudo
     CDS             complement(46693..47500)
                     /locus_tag="PWN45_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(47519..48319)
                     /gene="lpl2"
                     /locus_tag="PWN45_000210"
     CDS             complement(47519..48319)
                     /gene="lpl2"
                     /locus_tag="PWN45_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HDL0622011.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            complement(48350..49123)
                     /gene="lpl1"
                     /locus_tag="PWN45_000211"
     CDS             complement(48350..49123)
                     /gene="lpl1"
                     /locus_tag="PWN45_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDL0622012.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            49491..49799
                     /gene="spn"
                     /locus_tag="PWN45_000212"
     CDS             49491..49799
                     /gene="spn"
                     /locus_tag="PWN45_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDL0622013.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(49902..51410)
                     /locus_tag="PWN45_000213"
     CDS             complement(49902..51410)
                     /locus_tag="PWN45_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDL0622014.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(51432..>51634)
                     /locus_tag="PWN45_000214"
     CDS             complement(51432..>51634)
                     /locus_tag="PWN45_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDL0622015.1"
                     /translation="KELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKK
                     LQTHRMGDVIDGRNIEKIEVNL"
BASE COUNT        15877 a        10406 c         7064 g        18287 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatggcgg aggaagaggg attcgaaccc ccgcgagccg ttaagcccct gtcggttttc
     9061 aagaccgatc ccttcagccg gacttgggta ttcctcctaa acacaatatt tatcatatat
     9121 gaacgttctg taaaagtcaa tattgttttt aagcattaca tgattaccac aaatcattgt
     9181 cttccactac agttgaccta taaacgaatg taaaaaatca ctacttataa ttaatctatt
     9241 tgttgtctta caaacctttg tttttaggtt acaaagatgg catgtgacgt tgatagatac
     9301 tagatttttt caaactcgat tcagcatgtc acaacgtcaa cgtaaccacc atttccatac
     9361 aaagattata cacaaacttt actagctaaa tactgatcta ctgctttcgc tacgcctcta
     9421 ccttctttaa ttgcccatac aactaaactt tgaccacgtc tagcatctcc agcagcaaat
     9481 accttttcat tattagtttg atagtttgta tcatccgcca cgattcgatt tctatccgtt
     9541 ttaatgttaa aagcattcgg tactgttggt tctgtacctt cgaagccgat tgataataat
     9601 acaaggtcag caggccaaaa tctttcaggt ccttctttca tgaccatacc attttcgcct
     9661 tgctctaaaa tttgagtata caaaccacgt atgtgtcctt tatcgtcaac atcgtaacgc
     9721 attgtttgaa caccatatgc acgtggttcc ttaccaaact tagcttcgta ctcttggtgc
     9781 gcatagtcca ttttaaacac cggcattgct aaaggccatg atgcattttc tgtaaatgta
     9841 attgcttctg gcaatttcgt atatttatta aattgaacaa tcgatttaca attttctctt
     9901 aatgctgtcg ctacacagtc tgcccctgta tcaccagcac caatgataat gacattctta
     9961 tcttttgcag ttattgttat atcatcaatt tctccattta acaactgcgt ttgttcagtt
    10021 aaataatcca tagcgaaatg tataccatca cccatgcgtc cttctaaagg taaatctcta
    10081 cctttttgtg caccagtaca taatataatg gcatcatact cagattctaa cgttgcttta
    10141 tcaatatcaa cgccgacttc aacaccatta atgaaagtaa tgcccgcttc ttccattaac
    10201 ttaatacgac gtcgaaccac atctttatca agtttcatat tcggaatacc acacattaat
    10261 aaaccgcctg attctctagc acgttcataa atagttactt gatatcctag tagattaagt
    10321 tcttcagcag cagctaatcc tgctggaccg cttccaacga ttgccacttt ttcatctcta
    10381 cggcgactcg gaactttcgg cgctacccaa ccattttcaa aagcttcatc aataattgtg
    10441 cgttcaatac ctttaatcgc aatcgattct ctattaatct tcatcacaca agcactttcg
    10501 catggtgcag gacatacacg ccctgtaaag tcaggaaagt tatttgtttc gcttaagcgt
    10561 tcataagcag ttttaaaatc ttgatgatac actaagtcgt tccattcagg aatgtagttt
    10621 ccaattggac aacctattgt ttccctacca tactgttgtc cggtttgaca aaacggcgtt
    10681 ccacaatcca tacatcgtgc accttggata gaggcatctt ctttagtaaa tcgttgttga
    10741 tatgctttat gatgcttcaa acggtctacc agtgataatt cacctaagta ctgtttgtca
    10801 tacttcataa atcctttaaa ttcacccatc gtatctcccc ctttccttaa tacacaacgg
    10861 ctggtttatg tttagcatcg attgttttac tgtcatcgta aaatgcagct aacatcgctt
    10921 catcttcatt gtcatgtaat gatttgtgca aatgaatttt ttgcatcatt aattgataat
    10981 ctttaggaat aactttaacg acgacatctt caatgcgatc aaaatgtttt aacacatgaa
    11041 tcgctctcgt actattcgtg tgtgacacat gttcttccag catttgctta atgaatgctt
    11101 tttcttcttg gtgtttaatc tttgtaaacg aaagcgtatc tagttgatta ttttcaacaa
    11161 aagcttctac atcagacggg ataacgtaag caataccacc actcatacct tgaccgaagt
    11221 tcttacctac atcacctaaa ttaatgacat gtccaccagt catatactct aatccatggt
    11281 cgccgatacc ttcaacgaca acatctacac cactatttct aatacagaat ctttctcctg
    11341 cactaccgtt aataaatgcc ttaccacttg tcgcaccata gaatgagacg ttaccagcaa
    11401 taatttcatt ttgtcgttct tcaaaaggtg ctttgacaat gaccgtacca ccagataatc
    11461 ctttaccaac atagtcattc gcatctccag tatgatgaat cattaagcct ttcggtgcat
    11521 atgctgcaag actttgacca gcatgaccat tcgtataaac attaattgta ttttcaggaa
    11581 gtcctgcttc tccatattgt ttcgaaatct cactacctgt aataacccct acatcacgtt
    11641 gttcattatt tactgtaaag ctacctgtat agcgacgccc ttcagcaata tatggcttcg
    11701 ttacttcata taaatttgtt aaatcaaatc catgctcaag attatgattt tgttgaattt
    11761 cttttgtgtt tggcccatcg aaaggacata acagtttttc aacatcaata ctagccgctt
    11821 tgctattcgc ttttaatgtt gatgatcgtt gtaataaatc agttcttcca actaagtctt
    11881 ctacacgttt caaacctaaa gatgctaaaa tttctcttaa ttcttgtgca ataaaatgca
    11941 taaaattaac aacatgatgt gctttacctc tatataaagc acgtaaatct ttgttttgag
    12001 ttgcaactcc tactggacat gtatctttat ggcatacacg catcataata cagcccaaca
    12061 ccactaatgg tgcagttgca aatccaaatt cttccgctcc aagcgcacat gcgtacgcta
    12121 catctttacc agttaataac ttaccgtctg tttctaactt aacacgactt cttaagtcat
    12181 ttagttttaa tgtttgatgt gtttctgcta aaccaatctc ccaaggaaca ccggcatgct
    12241 gaatactcgt tttgggtgaa gcccctgtac caccatcgta accactgatg acaattttat
    12301 ctgcaaatgc ttttgccacc ccagatgcaa tggtaccaac acctgttttc gaaactaatt
    12361 ttaccgcgat atctgcatct ttattcgcat ttttcaaatc atgtatcagt tgcgctaaat
    12421 cttctattga ataaatatca tgatgtggcg gtggtgaaat cagaccgata cctggcgttg
    12481 accctcttgt cttcgcaatc cacggatata ccttagtacc aggtaattga ccaccttcac
    12541 caggctttgc accttgcgca actttaattt gaatttcttt ggcatgttgt aaataatcac
    12601 tagttacacc aaaacgccca gaagcaactt gtttaatcgc acttactttg ttgcttccat
    12661 caacttgtac ttcataacgt tttgcatctt cgccaccttc accactatta ctctttccac
    12721 ctaattggtt catggcttgt gctaacgttt catgtgcttc cgctgaaatc gatccataac
    12781 tcatcgcccc tgtattaaag cgtttgacaa tgtcacttac cggttcaact tggtcgatgt
    12841 caatcggtgt acatgcttta aattcaagta aatgtctaat gtgatctgtt ctatttttgt
    12901 tcaccgcttc agagtatgct ttaaattgcg catagtcatt ttctttacat gcgtgctgca
    12961 ataagaaaat agattccgga ttaaaagcat gatgttgacc ttgttgtctc cattggaatg
    13021 tactacctga tgcaagataa ttatcatcac tttgttgacg tgctttattt tcagcatcaa
    13081 tttgatcaat cgaaatacca gataacttag actgtgtccc agtaaaataa cgatcaatca
    13141 catcatgaga caagccaatc gcttcaaata tttgtgcccc ttgataactt tgcactgtcg
    13201 aaattcccat cttagccatt actttaatga caccttctga caatacatcc gtatatgtct
    13261 taacattatc gacaacggtg ccttgtaacc cttctgtcaa tgtcagttgt tcaactgtac
    13321 gttgcgctag gtatggcaca attgcattcg cgccatatgc gagtaaacaa gcaacatgat
    13381 gcacttctcg tgtctcacca gatttagcga ctaaacttgt agacatacgt aaatctgctt
    13441 taataagtaa ttgatgcaca tgacttattg cgagtaacat cggcattgca aagccattgc
    13501 tatcaactaa tccactatca tctaacacta gaatttgagc gccttgcttt acagcattca
    13561 ctgcttctcg gcctaatgct tctaacgcat cttccaaatc cccttcatat accgttgata
    13621 aataagttaa tttaaaatgt tcctgatcaa tcgctgctaa gtgtgattca ttcaataccg
    13681 gccttttcaa ttgaatacga tctaaaaccg tttcgtcagg tgctagtaag ttaccttcgc
    13741 cacctaaata agaaagttca ctcgttacga ttttttcacg atacgcatca attggtggat
    13801 tcgtaacttg tgcaaacagc tgtttaaagt aattaaatag tgattctggt cgctcgttca
    13861 acactgcaat tggcgcatca tatcccattg caccgatagg atccttctta ccttctacaa
    13921 gttcctgaat atacttatga atctcttctt tcgtgtatgc aaactgacgt tgtaatttaa
    13981 ataacgtctc atctttccat tgcgaatctt gatattgtat attttcaaaa tcaaagtcaa
    14041 ctttatggtt atcaatccac gctttatatg gtaattctcc agcaatcgca ccttttaaat
    14101 cattattttc aatgacttta ttctgtttaa aatcaacaag caataacttt ccaggattca
    14161 attgaccttt aaaagcaaca ttactttcag gtacgtccac aacacccact tcagatgaaa
    14221 agacaataaa gttatcttta gtaatcgtat aacgacctgg acgtaatcca tttctatctg
    14281 taagcgcgcc aagtttgtca ccgttacaga acgaaatcat tgtaggacca tcccacggtt
    14341 ccattaaata actataaaat tcataaaacg cacgtacatt tgcatcattc gcttcattat
    14401 ataaccaagg ttcaggtatg agtaacatcg ctgccttttc tggctccatg gctaacgata
    14461 agaactctag cgcattatct acaatggcag agtcactacc atcctcatcg acaatttgaa
    14521 acactttatg ttgatcctcg ccaaataatg tttcgattaa tttatgttgg cgtgctcgca
    14581 tccagtttac attaccttta atcgtgttaa tctcaccatt atgcattaac atacggttag
    14641 gatgtgccct tttccaactc gggaatgtat tcgtactaaa tctcgaatgc actaacccta
    14701 gctttgattg atataaatca tccgataaat ctgtatatag ttttttaatt tggtctgatc
    14761 gtaaccaacc tttatataca attgttttgc gtgataagct cgtaaaatac aattctaaat
    14821 cgcactgagt cgaatagaac tctaattgtt ttctcgctaa aaacaaacgc ttttcaacat
    14881 cttcaatgtc cctaatatca ataaacactt gttgaatgac tggcatcgta tctgctacat
    14941 gtttagcaat ggcatcttta ttaactggta cattacgata accaagaatt gataaccctt
    15001 cgccttcaaa atatttttta aaaactactt catgttcaga acctaaaatg cgttctttgg
    15061 aaaaaaataa ccccacggca tattcacctt cacctgggat atcaaagtcc gttacatgtt
    15121 gtttgaaaaa tgcaaaaggt atttcagtca taatacctgc gccatcacca gtgatgccat
    15181 ctgcgccgac cccgcccctg tgatctaagc gtcgcaacat ttcaagcgat ttatcaatga
    15241 tgtcgtgaga ccttttatta tccatattcg cataaaaacc aataccacac gcatcatgtt
    15301 cctcacgata gtcatataag cctttaatta atttctcatt gtgcatgatg cacacccctt
    15361 ttttagaata ttctgatatt agttattaat gataattact ataatctaaa aaatttatct
    15421 tttcaatata taaaatagaa gatattcatc taaaaaatag aacatttatc aataatattg
    15481 ctataatagt tacaaaacac tcatttttgg aggtgcctat atcgctatgg aaattaaaca
    15541 attaagatat tttatcgagg tcgctaaaag agaacatatc tccgaaacag cacttgaact
    15601 aaacattgct caatcagcga taagccgtca aattacatta ttagaacaag aacttaatgt
    15661 gtctttattt aaaaagcaag gacgcaatat tacgctcaca tctgaaggga aattattatt
    15721 taatgaagct ctgcgtatta ttgaacattt agatagcacg attgaacaat ttcaaagcca
    15781 tggattaact aaaaataaaa gtatctacat tggctatgat gagagtgacg tatcccacat
    15841 gttactacca cttatccaaa cgtttcattt acaaaatgat acacatgtca taccgagctt
    15901 attagaacat gacacaatta taaatagcgt attaaatggg aatatagaca ttggtttcac
    15961 ggaacttaca ccagaaatac gtaaacacaa acaattacat atgctacctt tattcgaaga
    16021 gcactatcat ttatatgcgc cttcagacga tccaattacg atggcgacac acccaccatt
    16081 aattcagttt gagcatagtc atatttattg cttggcacca tttgccgaaa ctgtaaaaaa
    16141 acaattgcgc aagattacta aatcagatgt atacacaatc agtagccaac cacttgcgca
    16201 gtatctcctt cgtcaaaaag aaggttacat catttcatca caaaatatac atttaccaga
    16261 atctaaatca tggatagata taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc
    16321 tattacaaag gagccttata caaagagtga tattggcata ttattaacac ttattcagca
    16381 attaatgact aaaacgtcta cattccatta agtaattcga tgctatcaat gatgattttc
    16441 gttttacact tagcagtaat atctttgaaa ataaaaaatt aagggcgtat aatcaccata
    16501 ttaattcatt catagaattg gggttttgtt tttgaaatat agaaatttat ttacaaagcc
    16561 atttaatata attttaggtt tgttctgcat cgcattcatt ggtgctattt tatttacatt
    16621 caataatgag aacttttaca acaaaccaat tggtcaaatt atagatgtca aacatgtttc
    16681 ttcgacacct acaaaagatg cacaaaataa tcgtgatatt aaatataaaa atcaattaaa
    16741 agtcaaaata ttaaatggtc aatttgctgg agaaacgacg acgattaatc atcagtacgt
    16801 caaatcgcaa gcagattctg aggcatttcg cacacacgaa aaagtattat tacatatttc
    16861 taacaagccg agtgatgctt atattataga aaagaagcgt gataccttaa cggttatcat
    16921 cactggtttg ttcttattaa ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat
    16981 tttatcgtta atcttgaatt ctattgccat attaatcgca atttacattc atattcaaca
    17041 tagcaatatt aatttattct tactcatgac cattgccatg atctgttcaa caatactcac
    17101 tttattgctc gttacaggct ggcatatgcg tacactcatt accatagcca gtacaattat
    17161 cggaactttt ttgagtatag gtttaacaga attaatcata tatatggcag atggtaaagg
    17221 cattaaatat gaaacgatga actttttatc attaccaccg aaagatatct tcctagcctc
    17281 agtgttaata ggttcacttg gcgctattat ggatgttgca attacaattg ctagtggtat
    17341 gcatgaaatt ttacagcgta cgccacacat ttcaatgagg agatgggcgc ttgctgggcg
    17401 aaacatcggc caagatatta tgggaacaat gactaacatc ttattattct catatttatc
    17461 cggtgcacta cctatgtttt taatcttttt aaaaaatgcc aatacagtga cttatactat
    17521 ttcgatgaat tggtcgttag aaattgcgcg cgcactaaca ggtggtatag gtatcgtact
    17581 tacaattcct atcacaatat tgtttatgga aatatttgaa acattaagga gggcgaaaca
    17641 atgaatgctg tatttatatt ggcgttattg ctcctctttc tgatgataat tttcggaggt
    17701 aaaaagggat tcatctctta tttaacccta tttttaaatt tcgctatttt aattattagt
    17761 attgtattga ttatttttgg cgtaccgatt tatgtcgtca cattcttttt ctgcattatt
    17821 attggcgctt gtaatttatt cgtactcaat agttacaatg ttaaaacaca agctgcattt
    17881 atcagcacta tcgttacaac aatattgttg attacactca tttatttatc ggtctacttc
    17941 ggtcatctac aaggcttttc aaccgaacaa caagatgaaa catatgtgta ttcaatgaat
    18001 attggcattg atatggttca atttatggtg ttcactatta ttcttgctgt tattgcagct
    18061 gtgatagatt taacaattac cattagttca cccatttatg aattgcatca agtgaatcca
    18121 acgcttacgc aatacgaatt attccaatct ggcatgcgtg ttggtagaga aattttagcg
    18181 acatctgcga acacgattta ccttgccttt tttggtggcc aacttgcttt atttatttgg
    18241 tttttcaaac ttaaatattc atttggtcat attataaact cgaaaatatt tgcccaagaa
    18301 tttatttcta ttattttagg cggtattgcc gttgcaatca gtattccgat tacggcttgg
    18361 attacggcat ttttaataaa aagaccatct cgacagcgca aaaatgatga gtctatcaat
    18421 taatcgcaac tagcaatata acttaagtta agcgacctgt ggatatatgt caattgttca
    18481 ctaggtcgct ttttactata gtaaattgtc cgatgatcta taacaaaaag cacctttatt
    18541 catcattaat tgttgtggtc gacgatgagt aaagatgctt ttaaatttaa tttattttat
    18601 agttaacgcc ataatatgaa caggtcttcc gacgaacata ctatctccca tatattgata
    18661 tccttgttgg cgataaagtg ccatggcatg tggattgtca gtattaaccg ttaacacaat
    18721 ctcattaata tcctgaaatg ttgaagtgat aaatgacgcc aatttttcca ttaccacttt
    18781 acctattcct ctattacgat aacgttgatc aacactaaat gacctgaaaa atactgcatc
    18841 ttggttatcg ctaaatggtg cgaccccttt tccacgatgt aatgtaaaaa aggccacaca
    18901 ttgcctgcca tccattacaa cgatgggata cctttcatta tcagacattg cagctgtaat
    18961 attttcctct ggcgttttaa caaacttcaa atcactttca cttaattgaa aagcttctaa
    19021 catcgaataa tcttgtggga cataatgtcg taacatgatg gtgtcattat ttgtcatgtt
    19081 taccaccata agtttcaatc caaactttga cagcaggcgc aatcaacgca tcattatctt
    19141 tatcaatcca gcgtatatcc gtaatttcat tattgatagt tacgttttcc caatcgattt
    19201 tggttaatgc gcgaaatcca tttaactcag ttaacatatc ctgttgtgga tatgcaggac
    19261 ctacaattgt cccgacatat tcaatttcat ccattgttaa tgttaaattt aattcttctt
    19321 ttacttctct taacagcgcg tgtacttgtg attccccttc ttctatttta ccacccggga
    19381 aataatactt ttcgcgattc cttacttgaa caagcaatat tttatcagct gtttcttcaa
    19441 ctaaacagac acatttaatc atctttgaca ccccaacttt gtgaaatcaa tttttcaaat
    19501 tatactgtac aattatgtta tcatatatga gtagttatag cgcaaaacgt tagcaattca
    19561 gcgcacccaa cttttcatat aaacagaaga tactaggggg aattattatt atggcaaaac
    19621 gttccaaatc acaacgttta tcaagtttac taaatgtcgc aggtttcata gtcgacggct
    19681 acaatggcta taaatatcat gctaaaaata aaaaattagt atatctttca ttaggtttaa
    19741 gcactgtagg aaccgtgtta gacttttaca tttcaattaa gtcaccacgt aagttcaaaa
    19801 aagcagtggc agttgttact ttaataacaa acggtgctag attatttaca agcattcgca
    19861 aagtaaaaca tgaatactaa ttcagaaaag gattggtcga acatagaaca tgaagttcat
    19921 tcgaccaatc ctttttcata tacaaaaatt cctattacaa caatcacgac tttgatagcc
    19981 ccgccataaa atttaggatt caatccaact tttcagcttg tgaaatgtaa taggaattca
    20041 ttatatattt atatacgtaa gactttagtg aatatatcta taattattta cttggtaagc
    20101 tggtaccgtt ctgtaagtta aaatacctgg tgcagctgaa tagttcattt ctgaaactaa
    20161 aatactacca tcgttattta cacgttctac aaacataaca tgaccatagt aacctacatc
    20221 tgtttgagcg atagaaccta cagtaggtct attgtcgata gtgtaaccat ctgctgccgc
    20281 tgcgttatcc cagttattag cattccacca ataagtacta atacctttac caatttcagc
    20341 acgacgatta aatacatgat atgtacattg accccatgta tataagtttt ggtgactgaa
    20401 tactggtgta ttgtaacctc tatttgtcgt tgttgcagat cctgagttcg tagatgcatt
    20461 accagttact ttcaattttt gacccggata tataaagaaa ttatttaagc catttaagct
    20521 cataattttt tgataagttg taccatattt tgatgcaatt aatgacaatg agtcacctgc
    20581 ttgtactgta tagtatgatc cgccacctga gttcgttgat ggacggctac tattgctcgc
    20641 agcgttactt gagctagcag tacctgatac ttttaatttt tgacctggat aaataaagaa
    20701 attatttaaa ccattaagtc gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat
    20761 taatgataat gagtcgcctg cttgtactgt gtagtatgat ccgccacctg agttcgttga
    20821 tggacggcta ctattactcg tagaattact tgagccagat acttttagta cttggtttgg
    20881 gaaaattaga ttagatgtta aattgtttaa tgactttaat ttagcaatcg aaatcccata
    20941 cttatttgaa attgcccaca ctgattcacc cggttttact gtgtgagttg tagccgcatt
    21001 tgcttgagtt gccgcaacag cgctaatcgc gcttgtccca ataatagctg caattacttt
    21061 tttttgcact ttaaaatcct cctcttgctt aactttccta acattctttt atccgaattt
    21121 atgaatacta catcattata cgattttatt atgtataata ggttgatgtt tgatgacatt
    21181 atgattacaa aaaaatcata tactgtatca tcaaatttta taattacccc ttaaaattat
    21241 tacaacttat tagattttac aatatctaaa ttattacaat ttcataatat ttcactataa
    21301 aatgattaca atccctttct ctattggaaa taattttatt ctccaacaat aacgccctac
    21361 aaacataagc atgaactttg cttgtaggac gcaatataat ttattttgct aatgacgttt
    21421 ctattgcctt aatctcatct ttagataaat taacaggttt ctctccatct ttgacatctt
    21481 cctgcaacgc tttttgagct tcttttgaat gatacaattc tacgatttta gcatatgttt
    21541 tgttatccaa gtctttgtca ttaactgcaa caatattaat atatggcttt actgcatctg
    21601 aatttgattt ttctaaaaat atcggatcat ttttaggatc tttacccgct ttagttgcta
    21661 caccgttatt aataactgca atatcgacat cagataaagc acgtgcagtt tgttgtgcat
    21721 ctactgcagt aatttttaaa tgttttggat ttgacgttat atctttcacc gtgcctgcta
    21781 atccgaaatc ttttttcagt tttattaaac cagctgcttc taatagttta agtgcacgtg
    21841 cttggtttga cacatcattt ggaatgacaa ctttagcacc atctttaacc tttttgacat
    21901 ctttaatttt atctgagtaa atgcccaacg gtgctaaaac tgttgtactt aatgctgaaa
    21961 tctttgttcc tttatgcgcc tttttatatt gatctaaaaa tgcaaaatgt tggaatgcat
    22021 tcatatcaat atcaccatca tttaatgctt tattcggtaa attgtaatct gagaagtgct
    22081 taatctccac atcaatatca tcttttttag ctaattcttt aaccttctcc caagccttag
    22141 tgtcatttga tgcgacacca attgttactt ttttatcatt gttaccacca cacgctgcta
    22201 ataatacaag tgctacgata actaacccaa tcaatctttt cattctatca attcctttca
    22261 aaatcttcac tatatatcat taatgtctac gtatgaatct agctagaaca ttccctagcg
    22321 tttgaatcac ttggacaata atgactaata caataacggt aataataatg accgtcgtat
    22381 caaatctttg ataaccatac actaaagcta agtctcctat accaccaccg ccaacagctc
    22441 ctgccatcgc cgtacttcca ataagtccaa taatcgcagt ggtaattgct aatactaacg
    22501 aacctaaagc ttcaggaatt aaaaaatatc taatgatttg tagtggtgaa gcgcccatcg
    22561 ctttcgccgc ttcaataatc ccctcgtcta cttccaataa tgagttttca acaagtcttg
    22621 caatgtaagg tgccacatat actgttaaag gcacgatggc agcagtcgta ccaattgaag
    22681 tacctactac taattttgtg aatggcacaa tcgcaattaa caaaataata aatggtagtg
    22741 accttaaaat attgattaaa ggatttaaaa cttgatgtat cactatattg ggccatatgc
    22801 cttgttttcg agtaattacc aataagacac ctaatggaat accaatcact gctcctaaaa
    22861 ataaagcaat agataccata tatagcgttt cgtacaatgc ttgtaataac tgtgcactgt
    22921 ctaaatcaga accaaacata tgttaatgca cctcctcaaa ttgaatattt ttctctttga
    22981 aatattgatt tattgccgtg tcttcaaatt gttgatccat attaaatcga agccacatat
    23041 aacatacggt gttaccttgt atttctgaca tagatgaaaa taaaatttta acctctctgc
    23101 cacaaatttg aatcaagtca tttataatcg gttgtgtcac ctgagtttcc tcgacgaaga
    23161 ttttataatc tttaaaatcg ccaacttgtt cgtcattcaa tcgacgaatc aatgatgtac
    23221 ttggctcagt ctgtataact gtagacacaa aattttgagc aatcgtcgtt ttaggatgac
    23281 taaacacctc tttaacagtt cctgtttcaa ccactttccc cttttccatt acagcaacac
    23341 gattacaaat gtctttaata acgcgcattt catgtgtaat catcataatt gtaatgccaa
    23401 aggtttgatt gacattcttt aataacgtca atatcgaagc agtcgttgct ggatccaatg
    23461 cgcttgttgc ttcatcgcat aggagtattt tcggattagt aacaagcgct cttgcaatag
    23521 ccaccctttg cttctgccca ccagataatt catcaggaaa ttggtctttt ttatcactca
    23581 atcctacaaa ttcaagcatt tccgttactc gttgcttaat ttctgttttg cttttcttac
    23641 ttaaaatgag tggcattgct acatttttaa atacggtagc tgaatttaat aaattgaaat
    23701 gctgaaatat cataccgata tctttcttaa tatccctcat cattttatcg ctataattcg
    23761 taatatcatg tccatctaca atcacttgtc cattcgaggc agcttcaaga tgattcacga
    23821 gtcttaccaa cgtacttttt cctgcaccac tatatccaat cacaccaaaa atatcattgc
    23881 gattgaccgt aaatgatacg tccttcaaag catctatttt ttgcttcttt ttattaaagg
    23941 tcttacttac ctgtcgaaac tcaatcatca tcgcccctcc tacaaattct tattaatcca
    24001 ctaggaataa gagcattata tgtaaaattg catatatcgt caatacaatt tgccgaattt
    24061 tctaaaaaat taaaaaataa gtaattcatg tgacaatgac gaattgtgag actactatga
    24121 catttatcaa attaaatcca taaaaatgtc caccaatcct ccacaacgca attactaaat
    24181 attaacatcg cacaaaaaag cactagcata ttcaagaaca acaaacgttg aactcaaaat
    24241 atatgccagt gctgctatta tttataaagt atctagtgct tgttttaaat catcgactaa
    24301 atcttcagta tcttcaatac ctacagaaat tcttacaagt ccgtctgtaa taccttcttt
    24361 agctcgaata tctgctggaa tggatgcatg tgtcatcaat gcaggtactg aaattaaact
    24421 ttccactgca cctaaacttt cagctaatgt gtaatacgat gttgctttaa tcaattgttt
    24481 ggcactttct gtatttttca cttcaaatgc aatcacacct gtatggccat ccgcttgagc
    24541 catatggaca tcatgattta aatgactttc aatacttgga tggaacactt gttgcacagc
    24601 tggatgtgct tgtaacattt taataatttc aataacgctg cgattaattt gttccatacg
    24661 taaacctaat gttttaatac ccctcacaag taaatagcta tcttgaggtc ctaaaatgcc
    24721 acctgttgaa tttgaaataa atgctaaacg ttctgcaagc ttgtcatccg atgttgcaac
    24781 taaaccagca acgacatcac tatgtccacc taaatatttc gttgcagaat gtaagacaat
    24841 atcgatacct aaatctaatg gattctgata ataaggtgtc ataaatgtgt tatcaacaac
    24901 tgaaatcaaa ccgtgttctt tcgcaatttc agcagacttt ttaatgtcag taacacgtaa
    24961 taatggatta gaaggtgttt caataaacaa catctttgtt gttgggcgta tcgcttgtac
    25021 aattgaatct gtatgcgttg tatctacaaa atccacttca atgccaaatc gtgtaaatac
    25081 ttttgtcaat gcgcgataag taccgccgta tacatctgaa tttaaaataa tatgatctcc
    25141 tttgtccaac agcataacaa ctgcactgat tgctgcaaca cctgaactaa atgcaaagcc
    25201 atgtttgcca ttttctaatg tcgcaataac gctttctaca gaacttcttg ttggattcgc
    25261 agtacgagaa tattcatatc cttgacgtaa atcaccaata tcatcttgta aatatgtact
    25321 tgtttgataa attggtgttg taacggcacc tgtataatcg tctgttgtgt gcccaccatg
    25381 aattaattta gttttcttgt tcattattat tctcctcata attaaatatt tgcttagaca
    25441 tatatcgatc actaccatct ggaaatacga caacaatcgt accttcagat aattgcgctt
    25501 ttaaattcaa tgcaccttgt aatgctgcac ctgaagaact gcctactaac aacccttcat
    25561 ttatagccaa acttttgaca tttcgaaagg catcttgatc tttaatcgta aatatcccat
    25621 ctacaagacg tctctctaaa aatatcggcc atttctcaga accgatacct tcagtgtcat
    25681 gtgcatgagc tggccctcca tttaacacgg acccttctgg ctcaacggca taacattgca
    25741 cgtgatgttg ctttaaataa cgtgcggtac ctgtaaatgt accgccagag ccaataccag
    25801 ccacaaaata atcaatttgc tgtaatgctg aagtcaattc gggtcccaat gtatgaaaat
    25861 atgtatccgg attatgttcg gattcaaatt gattcatata aacggcacca tatttttcag
    25921 cataggaacg tgcagctaat tgtgccccat gcataccttc agactgactc gtccttgaaa
    25981 cttctgcacc aagcgctatc ataatattaa tcttttcttc tgaaaaacca tacggcgcaa
    26041 agatcttaca tttcaaatga tgtctattcg ctgcaatagc taaccctatg cctgtattac
    26101 cagcagtcgc ttcaacaata gtttgacctg cacgcacacg cccttcttga attgccttct
    26161 ctactaaata tttcccgagt ctgtctttaa cactgcctcc aggattccat tgttcaagct
    26221 tggcataaat tttaacttta tcatcactat aatgttctaa cagtactaat ggtgtattgc
    26281 caattaaatc ataagtaatc atagatgcac cctcatctga catgccgatc aaatgaatga
    26341 aacctttctt catgtctcaa ttttaattct tacttttcag ataagaatta taaacgacat
    26401 tttgttattt tgcaattatc taagtttcga ttaattcaga accagtacta aattttcaat
    26461 tccaaacaaa aaaacacctg agcaacacaa atacttgtgt gtcagatgct tctatatatt
    26521 aactaaataa ttgcacgata aagactaaaa taataacgac aggcatcgca tacttaatta
    26581 agtaatacca accactgaat aatctaaatc gatctttacc aaaatattgt tgtaataatt
    26641 ttttatctaa taattgtcct acgacaagcg tagtacctaa tgcgcctaat ggcatcaata
    26701 cattcgaaac gatgaaatcc atattatcaa aaatcgttcc cgcaccgaat cttacatctt
    26761 ttaagatacc aaaagataag gttgctggaa tactaatgat aaatactaaa atactaccga
    26821 tcactgcgac ttttttacgt tttgtattgt cattcttcgt gaagttagaa acatttaatt
    26881 ctaataaaga aatagatgac gttaaagccg caaataagaa cagcactaag aatcccaaat
    26941 agaataatgt gcctagatgc atttgactaa agaccattgg cagtacttta aataataatc
    27001 caggcccttc ttgtggttca tagccaaaac tatgtaaagc cggaaatata gctagacctg
    27061 ccaatacaga tacaaagata ttcataacaa cgatagaaat agctgatgac ttaatcgtca
    27121 tgtctttaga ggcataactc gcataagtaa tcatacctgt agttcctaat gataacgtaa
    27181 agaatgattg acctagcgca aacaagatgc catcagcagt aatctctgat actcttggtt
    27241 gtaaaataaa tttcacacct tctaaggcgc catctaatgt taaagactta atcacaatga
    27301 cgattaaaaa gacaaacagc aatggcatca taactttcga tgccttttct aatccttttt
    27361 caacacctaa catgacaata atcatcgtag cgaatatgaa tataccttgc cctagaacgg
    27421 ttaaccaagg atttgatatt accgcttcaa aattcatttc ttggagatga ttgatgcgtt
    27481 gaaatataac taattgccat aatacttgtc cgatgtaaat gacaatccaa ccaccgataa
    27541 cactatagaa gccaaataaa ataaacactg ccaaattacc gttccagcca atgatattga
    27601 gccatttttt accagttaat ttactatata tttgtgttgt atatgtccgt cccattttcc
    27661 caacagtgaa ttccataatg agtaatggca acccaacaaa aatggtgaat attaagaaca
    27721 tagctagaaa ggcaccgccg ccataaatcc ctgccatata tgggaatttc cacatggcac
    27781 caagaccgat tgcagaaccc gcactagcta aaataaatcc agttgatgac ttccattgtg
    27841 attgttgtct tttcatcatt cacatctcct ttaaagcgtt aaagcgtcta cgtgtttaag
    27901 taacactcta acatgtatgt aaataacatg caatacacct gcatcaaaat tgtttcatta
    27961 atacggtagc acctggattt atcataatat aatcaactct tgtaatgtgt tgtcattacg
    28021 ctacattaaa ttttctggtt caacgctgta tacgtgcgcc aatatctcgc cacatacaac
    28081 accatctata tccccaacat ctcgccacat aacaacttca ggtgcaatgc ccataaaatt
    28141 tccacgacat tacaatacaa atcaacttct ggtataactc cataatattt ccacgacatc
    28201 acactacaaa ttaactttag ctaagttccc tatactatca cgccacaaaa aacactcaaa
    28261 tgacaaatat aataacatcg tcactcaagt gttaattttt aaaatcttca attatgttaa
    28321 agcattgaaa aagcgaataa cattttcttc taaaatatct ctgccttctc catgcgtcat
    28381 taaatgatgc gaatccttca gaccatttaa ttctttgtct actgatccta aatgttcata
    28441 aataaaatgt gcacttgttt cataggatgg cgcatcttta ccaccaaata atatattagc
    28501 tggcatttta ataacattta aatttgtcat gatatcatca ataaaatgtt gaaacttcgt
    28561 aatttcacct tcataatcat caattgctgc taattgcgca ctcgatgctt gctgatctaa
    28621 atttaaaata ttcgacatgc gttgactata ataaactaaa tgttctatta agccatcgtc
    28681 actcttttcc tttggtgctg acatgacagc gatacgtttc aaaggatagt gttgcgccaa
    28741 ttttaatgtc attaatccac ctaaagacac acccgttgca ctgatagatt cataaccttc
    28801 attgactaaa aattggtaag ctttctcaac ttcttcccac caatcatcta cattatatgt
    28861 catgaaatct ttcaacaata aaccatgacc tggataattc ggtgcataac aactaaatcc
    28921 ttggtcattt aactcagctg caagatgctt cacatcccga tttgtacctg taaatgaatg
    28981 taataataat atcgcatgtc catttgtgcc ttttaaatac gatggactcg gtgttttaat
    29041 tctcattttt ctatatacct ccactatgtc taaagatgtt tgctaaacgc gttgtcgtcg
    29101 atgattaata aagtatgggt ataccattaa gaataacgct acccaaatga ttgctagtga
    29161 cgtgccgcca atgacatctg aaaagtaatg tgcatgaaaa taaaggcgac aaaataatat
    29221 gctaagccat aatattccca taaccaacgc actcaacact tttgttattg tcttagcagc
    29281 aagtgaaata ataatgatca ttaaggcgaa atataataat gtgctggcgt tggaatgtcc
    29341 actcggaaat gaaaagcctg tatcaacggc taaatgatta tatggtcttg gacgtaatac
    29401 agtatcttta attaatttgt tcatgatgac acctgaaacc aaatatgtca caaaccaaac
    29461 cgctaaatgc ctctgtttaa taaacagtat gattgtgacg ataatggaaa tcaacacgac
    29521 acctttgaca tctccaattt ccgcactaaa cgtcatatag taattaaaca aattgttaac
    29581 atactgacgt tgtggctcac cgaaataatc tgtaaaccat gttaatgatc ccatatctat
    29641 atttttaagc cattcttgat ttgtcactac actgtaaaac atacctataa atacaatcag
    29701 cgcgattaaa aataaaggca ctgtcatttt cggtgatgtt aattttttat ctatcatctt
    29761 acaatctcct cgtatcatca ttttcatttt acaaatgtta tccataatat caatgtgcca
    29821 caaatttcac tttaccgaca atatcaaaat tataaagttc atattgttat gtatattgca
    29881 aataaaacat tgtataattg aaataacaat attttgctat tttcaattta gtacgattta
    29941 tatttattat acagaggggg taaggcgtat caatagagtt atttttgtct atatagcgtt
    30001 aatcattaca ttagttagat tctttacccc tattcatcca tcattttcaa atttgattta
    30061 ctggatattt gtattatatt ttattcctat tatactatgc gttatcggtt tcaaggccga
    30121 aaaacttatt gcaacaatgg tcattatacc taatttttta ggaatacttt atcgattata
    30181 tgcctacgtc acacatatac tctttatgta aaaggatagt gggcatgtct cgcaacaaat
    30241 atgcttgcgt cgacttgtca ccattcgcaa attttatgac atacgccttt cacgggctat
    30301 attcagaccc acgcattcat ccacgtaata aacacatcat gtaatagaaa aacagcacac
    30361 ccaaatatat ggcgttgcgc tgtttaacca agcatacttc tatagcttta ataagccagc
    30421 agaagcatac ctaaccttct taaatatgct tttccaaatt atcctcaagt ttgaatacga
    30481 taatacgttc acctgtaact gtacttaaat cactatggaa gctcatcact ttgatacctg
    30541 taattttaaa aatgatatca ttcaaatctt gctcaccgga ttcaactaat tcagaacgtg
    30601 ttcgtttaat atttaataat ccttcattcg tactacatac acgatattca gctggcgtta
    30661 agataccttg taaactaata atcaccatat ctcttaaaat gtctgattta actgacaagg
    30721 aacctctgcc aaggaaatct ttttcccatt gcgtaatggc tttactgatt tcagcttcga
    30781 tttcaccttt cgttcttttc atatcactaa ctccaataat attaaaattg attacttcat
    30841 ctttgtatcg ttatcacgac atcaactatt acattaagtt tatcattttt agtatatttt
    30901 aagaagctag aacattgtag atatgatgat atattagtta cttagcatcg caacatatca
    30961 tcgttaaatc caacttttaa aacgcccttc ctcattaacg ctcattaaac gcagccaatg
    31021 attagacacc ttcctagcga aatgctcatt attcgcgagt agtcttgcta caacatagtc
    31081 gggtgcctga ataacgacaa gtaaacgaat tggcgaatga tacatcgtcc gatcagcagc
    31141 cataacagat tgccatgata agccatacat cagatcactc gcattacctt gcatgacacc
    31201 aacacctgac gtgacggttt gtgtcgcttt attcccactt ccgtaaaaat gcggcgcaac
    31261 tgtcgacgca taatattgta aattaatcca ttgtgccaca agtgccggac cagaaatgat
    31321 ggtatttaat aatgtgccat ctttatcttt acgccaatca taattgtgta aaaatgtccg
    31381 cccttctaaa tcaatgcctt ttgttaattg gcgtcgtcca attataaatg atgcattttt
    31441 agccaatccc cattctggac gtacctcact ccaatcactc gcaaaccgct gcgcttcttc
    31501 cactggatga ttcacacgac caatcgttgg cagtttgtcc aaacgttcgc gattcgcgtg
    31561 ttcagaaatc atcggcatcg cgtcattcaa tgattcatat gcatctaaag caatagaaga
    31621 taatgtgtct ggcacatata cccatgccaa cgtatcagta gacgtatgat gttctgctac
    31681 cgcaaaaaca gttgtctctg gaatatacac acctgattgt tttaatcctt gtctgacatt
    31741 tggacgatta catatcatcg ctaataactt agcattaaaa ccgcttgatg cgccaccaca
    31801 agccccacat tcaagtgatg catgatgtgg attattgtga gaatgactag catgacctgc
    31861 taacacaacg aacggcgcaa atgcttcggt taaatccatc aatttcaacg cttgtaacgc
    31921 gaaatcaatt tgctcttgct cagtaaatcc aacaggtaag tctgatgttc ggtcaaactc
    31981 acgatcaatc gtcaactttg tttcaggctt tttcaaccac ttttgtttta ttttttgtaa
    32041 agacgcgcga ctttttctag gcataatcga attgacaatg gtacttaagc ttaaaaatgg
    32101 cccacttaat tcaggcaata acagactagg catgacatta tttttcatca atttaaatgt
    32161 gtaaaacatc gatgacattg tctgttgctg ttgtcgataa acattcatat cgtagcggtc
    32221 tgcaaattct ttaatgcgat atgccggcgg taccatgaca ggtaatgaat catgtttgaa
    32281 ttgttcgtct acggcatctt tttgaatagg taatccaaag aagcctgcaa taccaatcgt
    32341 ttcaaagggc cctgctgctt cgatatgtct acgaaatggt tctgaacgaa catctataca
    32401 aaatgcaatt tgcgctttcg ttgatgtgcc catctgattt agctcgctat tattttcatc
    32461 aactgcttgt gtgtcattta acaatactga atgtggctga ttagcgttat cattttctga
    32521 gacatttact tggtttacat ctaatgcgcc cgccacactt tcatgacctg ctttaatttt
    32581 ttgttttaac tgagattcgt atgtcatttc ccaggcaatt agccataaat ttttaaatac
    32641 atttttattc atagttgctg caaaatgaat aaacgtttga atttcattga cgtcatgttg
    32701 tagtaataca tcgctaggca tatcactgta gtaacaccat gatgcaacag tttgcttaaa
    32761 ccaattttcc gatctacttt cacaatcttt agcgactgac ttaaactcat caccaactag
    32821 caattgttcg acaactaacc gaattgccaa ataatccgtt aacaaatgtt gttcaaagta
    32881 atgctgttgt gaacggtaat acaacatacc tgcccaaccc ggtaacgcca aaagatgtcc
    32941 ttcaacataa gcttggtagt cttcctgatc tattgaaaaa tgagttaata ctgactctat
    33001 cgtcatttca ggatcattgg gtaagccttt aatcacttgg cgctgtgctt tagtaaaact
    33061 atggtcatgt tgcgctaaat gcaaccatgc atggtaaaaa ctttgctcac gcttcggcat
    33121 tgtccaactc gatagaaatt gatcgatata aagtttcgtc catttaatca tttgacgatt
    33181 cacttgttcg ctaagtggct caccttgttc atctattatt gcatcactca tcggacgtac
    33241 atcatagtga tgatatgatt cagccatatc acgttttgat ttttctaata gtagatcagc
    33301 aacaacatca acatttgaat gattcatata tgatgcaggt acgtctttta atgttttaat
    33361 gttatcaata taaagattga tgtagtgttg cgggatattg tagtgatgtt caagtaacat
    33421 atcagtaaca agttgattaa agacactttc atctaattca ccacgtgcca cagcgctttc
    33481 tattaatgct ttatttggga aaatatccac atctcgaaca tcacgtaacc attttgcgac
    33541 atcttcaaac gtatccgctt ctaatccttc ccatggattt cgtgctgcaa aaatcgaaat
    33601 tggtgataat ggtgtaataa cacgtttcgc attttcaatg actgaattga tatttaactg
    33661 tgttgtcata cctttcacct cctataaata cttcttcaaa taattcggat gactttctat
    33721 cgctttcgag cgtgcttcac ctagattaac taaccacacg tacaataccg caaaagcctt
    33781 agagtatcga tgccgcgcca cccaaatact taataaactg ccaaagatta aaataacaac
    33841 actaatgatg acactcactg taggcggcgt tgtcgcatgt gttgttatat tttgtaatac
    33901 agcgtaaaaa taattatgtg tgatgacgta gataaatgtc acgattgcaa tcaaaatcat
    33961 accaacaaga cgtgccatgc gtcctttact aaaggctacc atttgattcc aagatacaag
    34021 taatgaccat cctagaatga gtgcacttaa cacttcatat gcacttctgt cactactcat
    34081 ccaaaataga aatgccacga taatagctaa tacacgtccc atgacaatcc agccataagc
    34141 gtctttagca gatgcttgtt ttggaatatt gaatcgcttc acgatagaac ctgattgtaa
    34201 aaataatgtt gctttaaaaa taccgtgcaa tattaaatga ataatcgctg ctgaatatac
    34261 acccaatgca cattgaacta acataaagcc catttgactc atcgtagagc ccactaattg
    34321 gcgtttatag tcaacttgaa ctaagctaat acccgatcct aacaatacag aaatactaga
    34381 aaggataagt aataatgata acgcaaatcc attatcaaat atcggcgcaa aacgagttag
    34441 aataacacca cctgcattca caattcctgc atgcataatt gccgatactg gcgttggtgc
    34501 cgttacagat tcaatcaacc atcgatgaaa aggaaattgt gctgccggta tcatgacagc
    34561 taatacaagt agtacattcg tcaacaatga ccatgtcgga tgaactatat gttgtggtac
    34621 ccgccactcg ccagtcgcaa tataaatagt tacaattgct ccaacgaatg caagccaacc
    34681 acataaaaat gtcatgcttg ataatttcgc agactcacgt ggcactttcc aaaaacgatt
    34741 aacgttcatc agcaatgtta aacataataa tgtaataccc cagcagagtg ccatcagtct
    34801 taagtcttca gacatccatg ctaaagatgc aaacgacgta atcgcagtga acaatggaaa
    34861 gtaatgtcta taatgatgat cacctagtaa atatcgcatt gaaaactttt gaataataaa
    34921 gccaagcgcc attacaaagc cagctaataa ccaagataaa cgatctattt taaatggacc
    34981 taagacatgt tgaccatgaa taccgaaaaa gccaatgact gcaaataata ctggcatgac
    35041 tagtatgtat aaatgtaatt taatatatct cattggcata actggtgcta aaaacaacaa
    35101 gccacttatc aatgcaatga taagcgcaat aacaaacagt gaaaatagca attgaaaact
    35161 taacactgca taacctcctt atttctaatc tctcgcataa ttgcttatgt ataaaaataa
    35221 aaacctacaa tagtagattc tgtacataat ggcagaaaat ttactattgc aggtttcagt
    35281 ttaactagac actgcatcac ggtacgttga tataccttgt tgcagtgttc tctttaagcg
    35341 tgctcccatg cacatatgta tataaaatgt tacttctgtc tgttcaattc atcttcataa
    35401 atatgctttg cctagacgag acctaacgtg ttattcgttt taaacttata acataaaata
    35461 taattaaatt tctgcttcat gtcaaattca tgagcttaac ctctattaaa ccaatgattg
    35521 taaagatttt gtaaatgcac ctgtacagtt aggcagtatt tcccgtcctt ttaaaataaa
    35581 aaattcgcag ttatgatcat aacaattcaa gttaggaaaa aaatcaatta cgcacaagat
    35641 aactatgtac aatgaagtta actcataagc aaaggaggta atcttaatgg gtatcatcgc
    35701 tggcatcatt aaagttatca aaagcttaat cgaacaattc actggtaaat aagatttcat
    35761 aacaaacaaa ggaggtcttt cacatgggta tcattgcagg aatcattaaa ttcattaaag
    35821 gattaattga gaaattcact ggtaagtaag ttataaaaat ctcatagata tgaacatctt
    35881 atttgaaggg ggccattcac atggaattcg tagcaaaatt attcaaattc tttaaagatt
    35941 tacttggtaa atttttaggt aacaactaat ctcaaacatt aacgatcaac aactcatcac
    36001 tatgttaaat caacatacag gaggacaaaa cgatggctat tgtaggtact atcattaaaa
    36061 tcatcaaagc aattatcgac attttcgcaa aataatttaa gcgaattgaa tacttaaaat
    36121 tctcaggcca ctataccaat agggatggtg gctttgcttt gtgttgattt attgatgtga
    36181 ggtgagtctt gttagtttgt tgcaaataaa tggtcttggt gttttttgta taggacgttc
    36241 ttagtgggac atacggaata ttcgtgatct ttgtagtctg acgcgttata tttttgtggc
    36301 gtgttttatg tttgatactc gagttctgag acattcatga tttggcatgc gaaatcttaa
    36361 tgattttcat gatctagcgc aagatatatt ggccacgtgc ggaattgcgt tgcacgttta
    36421 gactgaaaca ctcgtgtgac cgtaagtgtt aatagtacat tgatagctgc atttacttca
    36481 ctcattttta tgactgttaa acaatgattg taccttcaat taacagttgg tacgatggtt
    36541 ttgccatttt tcatcaacgt aaatataaaa aggactaaga cacatacatg tcctagccct
    36601 atggataaaa tgcaaatttc tgctttatca aaactatcac actttagata gattgaaaac
    36661 aaaaagatcc taagaacacc ttaacttttt attaattgtc ataaattgca aacaattaag
    36721 ccacaattca aaaatgatta tacttcattc aacttatcgt gctggtctaa tttgccattg
    36781 atatggatct tcaaattgtt gccaatctgc atcaatttct tgcgcattga ctaagcatgc
    36841 gtcgagttct tttgttaatt tttcttcatc taattctgta ccaataatga caaattgtgt
    36901 atgacgatcg ccatattctg gatcccattc agctgcgaca tcttgacgtt ctgctaatat
    36961 ttgtgtttgt tgcgcttcag acatactagc cacccaatat gtaactggat gaatattgca
    37021 agatgaccct gcttgagata ataaacatgc tacgtgattg tattgtgcta gccatacgat
    37081 accttttgat cgaacgacat tatttggcat gctttctaac caatcattga accttttagc
    37141 atggaaaggt agacgacgtt tatatacaaa cgatgatata ccatattctt ctgtttcagg
    37201 tgtatgcgat gcatgcccac cagactcaag ttctttgatc catcctgctg actcgctcgc
    37261 tttttcaaaa tcaaaacgct gcgtattcaa gacttctttt aaatctactt cagaatttgt
    37321 tgtcttaata attttagcag tcggttgcaa tgcgcttaac attttttcta acttcgctag
    37381 ttcttcttca ctaattaaat caattttatt aataatcaat acatcacaaa attcaacttg
    37441 gtcaattaat aaatcagcaa tcgaacgctc atctgtttcg tcaacgcttt gatcacgatc
    37501 catcaataaa tcttctgagt tgatgtcatg tacgaagcgg ttagcatcca caactgtaac
    37561 cattgtatct aaacggcaaa tcgctgtaag atcaatgcca agttcatcat caatatatga
    37621 gaaagtttgt gcaacaggta ctggctctga aatccctgtt gactcaataa caatttgatc
    37681 gatgccacct tttttcacta aacgctcaac ttcttttaat aaatcgtctc taagtgtaca
    37741 acagatacaa ccattagaaa gttcgactaa tttttcatct gtacgcgata gtcccccacc
    37801 atctgcgaca agatctttat cgatatttac ttcactcata tcatttacaa ttaccgcgat
    37861 acgtcgacct tctcgatttt gtaaaatatg atttaacaac gttgtcttcc ccgagcctaa
    37921 ataaccactt aataccgtaa ctggaatttt agccatatat ataccctcac ttcaatttat
    37981 ttgtaaatag gaataattcc gttttacatt atataggagc gtttcctctt tcgcaatctt
    38041 cgataataaa aaaatagtat acttaattaa attattgagc gctttacttt ataatggaga
    38101 caaagatata tctcacgaaa gagaatcgag gtgtataaac atgttatttg tcattttagt
    38161 tttatatgtt actggtattg catttattct actcagtgtt tttggttcaa agactgaagg
    38221 attatctacg aaacatactt tatataccat tggcagtgct attataacga ttgctatttt
    38281 catttcaatt ggctatgcca ttcaatactt aactgcagcg ctttatggtt tgtaaggtga
    38341 aggtgatgag taacgggtag ttcgggagag gttaacttgc gttgattttg ataaagtgat
    38401 catagctttt agtacttgag gatttttatt gttgctgtta cgaatgtggt catgtttaat
    38461 gcgggacagt aatttaagtt gtttttttac aattgagagt gtgatatttc gattcggttc
    38521 gaattacttt acatgggaat aatataaatt aaaaagaagc ggcctagtgt cagttgtgaa
    38581 tatactgaac attggtcgct ttatttagta gtatgatatg tagtttagct attaattttt
    38641 ttcaggtcat ccttaatgct gtctatctca gacatggcac ttttaaccca atctccttga
    38701 gctgcacctt taaaattagc tttaaaagct tcgcaatgtt gcgccatttg ttcaattaat
    38761 actttttctt caccttttaa tccgttttca atatctttgt atttatgctt atgttcaggt
    38821 gcaataactg tgcgaatatt ttctttttgc gcttccattt tagatatgag attaagtgtt
    38881 tctactgtag tacttatatc tggcattctt aaggtcatat ctggttctat tagagtcatt
    38941 taatctcctc caaattatca gtcacttagc ttatctaact gcttttcata agactttttt
    39001 aagtcttctt tatattcttc taatttccat tcttgctttc taattatctc attgatttct
    39061 tgctcatacg cttctatatt tctcgtcatt ttcctttggg catcttcact ataattcatt
    39121 tcgtgcttca aatattgaaa aacatcataa ctacgctcat tttcttttcg agttctatgc
    39181 ctaagttcat ttaaattttg cagttcattc tctatacgtt ttttctcaga ttcatatgat
    39241 tcttctaatt catttttatt attaatttga ttaaacatca tagtaatcca cctatttgtg
    39301 atgcaagcac ttgatcttta tcaacgattt cattaatact gagtctaact ctcataatat
    39361 aatttgatac agaagaccct atcgttgaaa gttgtctttc ttttgcagat atcatttgtt
    39421 caggttctct taccatacta ctttcattca cttgaccttc atctaaagca ttgagaactc
    39481 catttacact caaatcttca cctacagctt ttccagcttt ttgagcgtct tcccaatttt
    39541 ttccaaattt ctttttcttt tcattataca tttttttgat ttgattaatt tcatcgtcta
    39601 tcagttgact gaatgactgc gcaattgtta aagctgttaa actttctaaa actttttgct
    39661 gcgaagaaga taatcctcct ccatttgtag tcatcatatt tgctctaagt aattctatac
    39721 tagctaattt agagatagca ttatttgaat tctttacaaa tgacttatta ttttcttctg
    39781 cataaccttg tagtttttta agttcttttt ttatagcttt ttgttcttct tcggtcagaa
    39841 agccttccat ttcatgacct ttaccatttt taaagacttt atcattaccg aaaaaaacat
    39901 aatgcttata tttcttcaga ttttctgtca atgcatcatt ttctgcaaca aacctagtaa
    39961 tattaccttt ataattttta tataattcta ataaacgttt tccatctggg gaattaggat
    40021 tcaaactagt tatagaaata ggagctggat tataaaccac aatgttcgga ataccattac
    40081 tcattcctag aactaccgca tctcttccac ctagtgaatg tccagtaata aaatcaatgt
    40141 catacttact ttttatttta tttataaatt cttgtgtatt cgcatatcta tagttttgat
    40201 cagatgcagg agaatataat attttaagat ctgcatatcc atctttaact gtttccaaag
    40261 cattggtaac attttgtctt gaaaattcac cttcttttaa ctttttaaag gcttcgtctt
    40321 gtaaattagt cccagtcatc ccgagagtta ctttgcctgt atctttattt aaaaaagcag
    40381 aagttgttac tccagtaaga gaatcagtgt aactatcaac atattcaaga ttagaaggga
    40441 attttttcat ttttttcaaa tcattaaatt gattaatcac gtctcttttt gatagaccat
    40501 tattattagc attctcaact tcgtatgacg ctttcgaaat agcacttgta gactcagtat
    40561 tttgattaat tgcttttaaa tctattttat acttcctact caacattctc accttcagac
    40621 ggtccaaaat tcaaataatc tcttaatgtt gtctcttgac ctttatcttt tgaaaattta
    40681 tattctattt ttttatatcc tactgagctt ccttttatat caccatctat atataatttt
    40741 agatttggag agttattagt tgtaattgga taaatttctc taagtttttt tacattttta
    40801 tcactattgt tcattttata ttgtgcttca tagctcggta cgttttcatt agttgttata
    40861 tttccatctt gataattttt taaatcttta aaatcagcat actgactaaa aaatttaaaa
    40921 ttttcaattt tttgtttaag ttttttgtct tctacattat ccaaaagaac aatcttatta
    40981 ttttttagtt caacacgata ttttttctca ttatgatttt tgctaatatc atcataaact
    41041 ttatttacaa aataataacc tgttgccttt ttggcatttc tattaagata taacaccatg
    41101 ccttcatcat ctaatactcc tggtttgtta ctttttgaaa aacctgtaag tatagtccac
    41161 gtacctttat cgcctttttt aaactcacca tctcgatatc cttctttgtc gtataaatcc
    41221 tcgagattct taattggata catatctaac gttttcgcaa aactcttttt gatttgttct
    41281 tcttttgaat ctgttttatt cgtttgttcc tcttttttct gttcatcttt catatttcca
    41341 catcccccaa taaaaatgat taaaagtaat acactcatgt acaaagcaat tttttttata
    41401 tactccatca tttcacatct ctccgtttta attttataaa catttgaata tttcatacaa
    41461 cttattaaaa tagtgattat tcaactttta tgttatcaaa caaaactaac ttattcaatt
    41521 taatacatgt aattcatttt aactttatta ttaaatgttc taacgttatt ttttatatta
    41581 gtaattatat ttatttatcc tcgcttggat taaagtagat actatctgtg aagtaaatat
    41641 tttcttcctt gttttcaata aaggtaaatt caatattttt atatccgact gatgaacctt
    41701 ttaaattacc tgagcctttc aataataatt tgggcgcttt ttgcgtcggg atattatatc
    41761 gcttccttaa ttgttcaaca ttgtaatcac tgtttttcaa ttgatatttt gcagagtaaa
    41821 ttggtacttc tgggttatat gacacttcgt cctctttata gttttccaat tctttgaaat
    41881 tcccgtattg tacaaagaag ttaaattctt cgatttcttt ttttactttt tcgtcatcga
    41941 ttggttttaa tggaatcatt ttattatttt ccattttcac aggatatctt tttgtatgat
    42001 tgtgtgtcat tccatcgcta tcttcaacaa cttctctaac aatataatgc cctttagccg
    42061 ttctagtatt tctgttaatt tctaaaactg ctcctctaga ttccagattt tctcctttta
    42121 attggatttt catttcagat ctaattagcc aagtaccttt atcatctttt ttaaattcgc
    42181 catctcgata tccttctttg tcgtataaat cctcgagatt cttaataggg tagatatcta
    42241 acgttttcgc aaagctcttt ttgatttgtt cttcttttga atctccaatg atcttattat
    42301 caccatcaca agatgtaaca aagatgatta aaatcaacaa actaatgcac aatccaatcc
    42361 tttttataga cttcattgtt acacaccatc ctcatcattc atcatcttca tctaccttcg
    42421 ccggctggaa attcaaagaa tctattactg acaaatcgct ttttccacct tttgaaaagt
    42481 caatttccaa ctttttataa cccacagaac ttcctttcaa attaccatca atatgcattt
    42541 ttaataccgg tgctttatca gtaggaatat tataacgact tcttaattgc ttaacatttt
    42601 catctttatt gctcatttta aattttgcgt cataacttgg aacattctca ttaattgaga
    42661 catcaccatt gttgtagttt ttcaattctt taaggtttgc atattgtcca aaaaatttaa
    42721 agttttctat tctctttttt agatttgtat cttctacttt atctaataag ataattttat
    42781 tatttttcat ttcaacttta taattttttc tatctgggaa tttgccctta ttatagaatg
    42841 tcttaacaaa ataatgtccc tttgcagtcc gtgtatttct atctaagtat aagaccatac
    42901 cttcattact caatccacct tgtttattac ttttggcgaa atctgtatat atcgtccaca
    42961 tccctttatc acccttttta aattcgccat ctcggtatcc ttctttgtcg tataactcct
    43021 cgagattctt aattggatac atatctaatg ttttctcaaa actctttttg atttgttctt
    43081 cttttgaatc tgttttattt gtttgttctt cttttttctg ttcatctttc atatttcgac
    43141 atccaacaat aaaaatgatt aaaagaaata cactcatgta taaagccatt ctttttatat
    43201 accccatcat ttcacatctc tctgttttaa tttttataaa tatttgtata gttcatacaa
    43261 cctattaaaa taataatttt tctactatca tgtcatcaaa caaaactgac tatatgaatt
    43321 taatacagtt tgttaattta actttattat taatttttct aacgtttttg taatactaaa
    43381 tatacttatt tatcctcact tgggttgaaa ttaatactat ctgtgaagta aatattttct
    43441 tccttatttt caacaaaggt aaattcaata tttttatatc caactgatga gccttttaaa
    43501 ttacctgaac ccttcaataa taatttaggc gcttttttcg tcgttatatt atatcgctta
    43561 cgtagttgtt ctacattata atcactattt ttcaattgat attgtgcaga gtatattggt
    43621 gcttctgggt tatatgtcac ttctccgtct ttatagtttt ccaattcttt gaaattcccg
    43681 tattgtacga agaatttaaa ttcttcaatt tcttttttta ctttttcatc atcgattggt
    43741 tttaatgtaa tgattttatt attttccatt ttcactggat attttcgttc atcactatat
    43801 accttgccct cactgtcttc tttaactatc ctgacaaaat attcaccagt gcatgttcta
    43861 ctatttctat ttaaacgtat gaccatacct ttagatacca tattttcatt tttcagttct
    43921 gttgtcattt cagatctaat aacccacttc cctttgtcac cctttttaaa ttcgccatct
    43981 cgatatcctt ctttgtcata aaaatcttct agatttttcg tagggtatac atctaatgtt
    44041 ttcgcaaagc tctttttgat ctgtgtttct ttcgaatctc cagtgatctt attactttcg
    44101 catcctgcca ttaatacgat taaaattaat ataaatatgt ataatttctg aaaacacttc
    44161 atcttgtcac atctctttgc acattttttg atatttgatc atttcatcta accacacatt
    44221 ttaatttaac tttacttact tggtatgaat tcaacactat cgttaaaata aatattctca
    44281 ccttttcttt caacaaaagt aaattcaata tcgttttgac caactgagga attttttaaa
    44341 tctcctccac ctttcaaaag taacttcggt gtttcttttg ttgaaatgtc atatctttta
    44401 cgtagttgcc ttacattgta atcatcgttg tgtaattgat atttagccga ataaattggt
    44461 gcttcaggat tatatgaaaa ctcaccattg ttgtattttt cgaaattttt aaagtttcca
    44521 tattgcacaa aaaacttgaa gttttcaatt tcttttttta ttttctcgtc attaatttgt
    44581 tcagtaggaa taattttatt tcctaccatt ttcacttcgt atttttcctc cttatcttta
    44641 tttacacgat tattattttc acttatttct ttcaaaacaa agaaaccttt ggctgtttcg
    44701 gtatttctat taatatataa ttgcatacct cttgttttca tcactttgcc ctttggctgt
    44761 ttaactattt cggatctaat tagccaagta cctttatcat cttttttaaa ttcgccatct
    44821 cgatatcctt ctttgccata aaaatcctcg agattcttaa ttggatacat atctaacgtt
    44881 ttcgaaaaac tctttttaat ttgtgcttct tttgaacctt cttttggatt ttctgcagta
    44941 tcgctttgac tatcacaacc cattataaaa atggtcaaaa taataacact tattaacaat
    45001 gcaagccttt ttagatattc cattccttca caccctaata tttaattatc agtactattt
    45061 tttcgctggc ttataactta aataatcaat gactgaaata tccctacctt cttttgaaaa
    45121 atctatttct aaccttttat atccaacaga actacctttt aagtccccgt caatatgcat
    45181 ttttaacatt ggagcttttt cagtaggaat gttataacga cttcttaatt gtttaacatt
    45241 ttcatcttta ttgctcattt tatattccac gtcataactt ggaacattcc aatttattga
    45301 cacgtcgcca ttgttgtaat tttccaaatc cttaaaattt gcatattgtc cgaaaaattt
    45361 aaagttttct attctctttt ttagatttgg atcttctacc ttgtctaata agataatttt
    45421 attatttttc atttcaactt tataattttt tctatcaggt aatttatcct tttcataaaa
    45481 tttcttaaca aaataatatc ccttagccgt tcgagtattt ctatccagat ttaaaaccat
    45541 accttcatca tccaattcgt ctgatttatt gcctttagca aaatctgtat atatagtcca
    45601 cattcctttg tcgccctttt taaattcgcc atcacggtat ccttctttat catataagtc
    45661 ctcgagattc ttaattggat acatatctaa cgttttcgaa aagctctttt tgatttgtgt
    45721 ttcttttgaa tctccagtga tcttattatc accatcgcaa gatgttacaa aaatgcttaa
    45781 aatcaacaga cttatgcaca atccaatcct ttttatagac ttcattgtta cacaccatcc
    45841 tcattattca tcatctttat tagttttcgc tggctggtaa tccaaagaat ctattactga
    45901 taaatggcta ttttcacgtt tcgaaaaatc aatttctaac ttcctatctc caacggaact
    45961 tcctttcaaa ttaccatcaa tatgcatttt taataccggt gatttatcag taggaatatt
    46021 ataacgactt cttaattgct taacattttc atctttatta ctcattttat atttcacgtc
    46081 ataacttgga acattctcat taattgagac atcaccattg ctgtagtttt tcaattcttt
    46141 aagatttgca tattgactga aaaatttaaa attttctatt ttttgtttaa gctttttatc
    46201 ttctacttta tctaataaaa tgattttatt atttttcatt tcaactttat aattttttct
    46261 atcaggtaat ttatccttgc gataaaacgt cctgacaaag tagtacccct tagctgtgcg
    46321 tgtgtttcta tctaagtaca agaccatgcc ttcattactt agttcacccg gtttattact
    46381 tttggcgaaa tctgtatata tcgtccacat ccctttgtca cctttttcaa attcgccatc
    46441 tcgatagcct tctttgtcat atagttcctc gagattctta attgaataca tatctaacgt
    46501 tttcgcaaaa ctctttttga tttgtgtttc tttcgagtct tcttttgatt tttctgcagt
    46561 atcgcttgat ttatcacaac ctattataaa aacactcata accataataa ctatatacaa
    46621 tacaagtctt tttaaataac ccatcatttc acaactctct ttattctcaa tatttgcttt
    46681 tgttgactaa tatcaacttt ttgttatttt gttacttgga acgaactcaa cgctgtcaac
    46741 ataataaatg ctttcttctt tacttctact aaatatgatt tctatttcct tatatcctac
    46801 agaagacccc tttatatctc cagatccctt aaacaataat tttggtgtct tgctagtttt
    46861 tagattaaat ctctttttca actgttgaat gtttctgtca tttttcttca ttttatattc
    46921 tgcagagtaa ttgggagcat taggattata tgaaatatta gtaatctcat tccgatcaaa
    46981 atgattaaag tttccatatt gtgaaaataa ttcaaacgac tctatttcat ttttttaact
    47041 ttttgtcttt aacatctttt atcaaaacta tcctattatt caccaatttc actggatatt
    47101 tcttttcatt tttttgaaca tcatgatttt tatcttcttt caattctcta ataatataac
    47161 ttcctttagc agttctctta tttctatcta actttaatat cattcctcgt gattttaaac
    47221 tctcgccttt tggttgtgat accatagctg aatacaatac ccacgtcccc ttatcgcctt
    47281 tttcaaactc accatcacga tatccttctt tgtcatataa atcctcgaga ttcttaatag
    47341 ggtacatatc taacgttttc gcaaagctct ttttgatttg ttcttctttt gagtcttctc
    47401 tttgattttc tgcattattg cttgaactat cacaacctat tataaaaacg ctcaaaatta
    47461 taacatttgt gtacattcca attcttttta ttgaccacat tgtttcacat cctccctatc
    47521 attcatcatc tttatttttt ttggcaggct gaaaatttaa tgaatcgact actgataatt
    47581 cgctattttc ttcttttgaa aagtctattt ctaacttttt atatccaacg gaactgcctt
    47641 ttaagtcccc atcaatatgc atttttaata ttggagcttt ttcagtagga atgttataac
    47701 gacttcttaa ttgcttaaca ttttcatctt tattactcat tttaaatttc gcgtcataac
    47761 ttggaacatt actattaatt gaaacgtcgc cattgttgta acttttcaaa ctcttgaaat
    47821 cggcatattg tccgaaaaat ttaaagtttt ctattctttc tttaagtttt tgatcttcca
    47881 ccttgtctaa taaaataatt ttattgtttt tcatctcaac tctataattt ttactataag
    47941 agttttcatg attgtcatat atagtatcta caaaataata tcccttggcc gtacgtgtat
    48001 ttctatccaa atttaaaatc ataccttcat tatctaatac acccggttta ttacttttgg
    48061 cgaaatctgt atatatcatc cacatccctt tgtcaccttt tttaaattcg ctatctcgat
    48121 atccttcttt gtcatataag tcctcgagat tcttaattgg atacatatct aatgttttcg
    48181 caaagctctt tttgatttgt tcttcctttg aatcttcttt tgctttttcc ccagtatcgc
    48241 ttgaactatc acaacctatt ataaaaatgc ccaaaataat aacacttatg aaccatgcaa
    48301 gcttttttag atatcgcatt ttttcacacc ctaatattcc ttatcaatac tattttttcg
    48361 ctggtttata acttaaaaat tcaatgactg ataatttgct gttttcacgt tttgaaaagt
    48421 caatttctaa ctttctataa cctacagaac tgcctttcag gtccccatca atatgcattt
    48481 ttaatatagg tgatttttca gtcgaaatat tataacgact tcttaattcc ttaactattt
    48541 catctttatt gctcattttg tattcaacat cataactagg aacattttca ttaattgaaa
    48601 catcaccatt attatatttt ctcaattctt taagatttgc atattgaccg aaaaatttaa
    48661 agttttctat tttctgcttg agcttatcat cttttacttc atctaataaa ataatcttat
    48721 tatttttcat ttcaatttta taattctttt catctggtag tttaccattc cggtaaaatg
    48781 tagtaacaaa ataatggcct ttagcggttc tagtattcct atccagattt aaaaccatac
    48841 cttcatcatc caattcaccc ggtttattac ttttggcgaa atctgtatat atcgtccaca
    48901 tccctttgtc acctttttta aattcgctat ctcgatatcc ttctttgtca tataagtcct
    48961 cgagattctt aattggatac atatctaatg ttatcgcaaa gctctttttg atttgttctt
    49021 cctttgaatc ttcttttgtt tcattacctt tgccacaacc tgctatcgca aatattaaaa
    49081 tcataacgct tatgtacaat gcaaaccttt ttaaatatcc catcgcttca cgtctctctg
    49141 ttttatactt catttatatt ctcttttcat ataaataatt aattattttg tatatttctc
    49201 ttttacgtta ccaaaatata catatacaat caatttaatt tcttaattaa atttgtactt
    49261 attattatat tgcgtcattt ttttattatt gtttcatttg atttaaaccc tttttgaaat
    49321 caaggtttta ttaggaaaaa tcctcaagca tcgtattaaa gattaattgc atttaaacaa
    49381 tatttgtcac tttaactatt tattaaaaat tattaaatcg acttaataaa cgtaattttc
    49441 ttaatattca gtgttatgat ggagttacaa tttttagagg agtgatagat atgaaattta
    49501 aaaaggtttt agtagctacg gcaatggtag gtgttttagc aactggcgtt gttggatatg
    49561 gtaatcaagc agatgcgaaa gtttattctc aaaatggact cgtactacat gatgatgcaa
    49621 acttcttaga acatgaatta agttatattg atgtgttatt agataagaat gctgaccaag
    49681 ctacaaaaga caacttaaga tcatacttcg ccgataaagg actacattca atcaaagaca
    49741 ttatcaacaa agctaaacaa gacggctttg atgtttcaaa atatgaacac gtaaaataaa
    49801 ttcaataatg ctttgtaaca aagcacaaaa aacagctcaa tgacgtagaa gtcagtgatt
    49861 aactcactaa tttcattaca tcattaagct gttttattta attatttttc taattttggt
    49921 gccttacgtt caactttctt ttcacctaat ggataaaacc aggtaatcgg gaatccgtta
    49981 gtatcgtagc cgacaatttt atcaccatgt aatttatgat atgcaggtgt gttttccatt
    50041 ctttcatcaa actcacgatc tgctttttct tcaggagatt cataaatatc tatgttttta
    50101 gaaacaaaat ttccacttaa taattcacct gtaataggat caactactgt gaagtttagt
    50161 gtttctccat tttgtaatct aaatccagca tgatctacat caaagctgaa ttcacctttt
    50221 tcatcgactt gcaaatcagg gaattcttga ccttcaccta gttcagagtc taccttcaca
    50281 actgcatttg cgaatttagt cttaccttta attactttag taccttcttt atcaagaata
    50341 cctttacctt tttctgtatg atataagctt ccgtaaattt catctacatg taaatcttct
    50401 ttaacatctt tcaatacttt tactttttcc actttagtat ggtcatattt agtttctgct
    50461 ttatcaatat cttctaaact cgttacctta gttttgaaaa ttaatgactt caatgcctca
    50521 tcttcgtcat caggtgcaaa tgttaatgag atctcatcat ttttctttaa ctctaaatca
    50581 tcgaaacgtt tcgttttgaa gtcaaaatta aagtatcctt tgtcatcaat aggcatccag
    50641 attccttctg atccagattt cgcttcttct ttatttggac caccattagc atttgtctca
    50701 aagttaataa atttattatt aatagataga gcaactttac cttttacaga ggtatggcct
    50761 ttaataatac ctgaaccttc agtaataggt tcgataaaaa cttggtgatg tccatctttt
    50821 tcttttagct gttctttcgg tatttcatac gctttttcat atcgcggtgt tgtatatgta
    50881 gctttagctt ctgtactttc ttcctcagca ccaacttctt cagttgaagt ttcttctact
    50941 tcttcatctt cttcatcgtc acctaaatag ggatctgatg aagacacttc aatttcttga
    51001 ttatgaacaa ttttgcgacc atttaagtca tacttaaaat tcccatcttt atcagacata
    51061 acaaagctgc tgtcagaatc ttcaactgag ctcacatctt ttttatcaat agttaatagt
    51121 ataaattgat ttggtaaagt tgttccttgt atagaagtat cacctggttt aatatcattc
    51181 aatttgacaa aattagccgg tacttttgat gtttcattag tattattttg aggctgaaca
    51241 gcttgattta cattattctg tttttcctga ttgtctacat ttatattaga gattaaagcg
    51301 ttttgatttt cagatgcttc tgcttgtccg tttgtcataa tatataacat tgtaattgca
    51361 acagatacta aaccgacttt cattttacgt aacttaaaat tttccctcat gatatgctcc
    51421 ctcgaataat tttataaatt cacttcaatt ttttctatat ttctgccatc aataacatca
    51481 cccatacggt gtgtttgtaa ctttttattc aattcaaatg tgtagaaccc accatctttc
    51541 atagtaattc gaattttact gtctttaggt tctgtcttat aaaggttatg tttatcaatt
    51601 aaatgctttc ttaatttaaa atcaagttct ttta
//
LOCUS       DANJFZ010000004        43989 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.004, whole genome shotgun sequence.
ACCESSION   DANJFZ010000004 DANJFZ010000000
VERSION     DANJFZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.004"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            115..315
                     /locus_tag="PWN45_000215"
     CDS             115..315
                     /locus_tag="PWN45_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0622016.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(442..1011)
                     /locus_tag="PWN45_000216"
     CDS             complement(442..1011)
                     /locus_tag="PWN45_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0622017.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1271..1666)
                     /locus_tag="PWN45_000217"
     CDS             complement(1271..1666)
                     /locus_tag="PWN45_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDL0622018.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(1734..2087)
                     /locus_tag="PWN45_000218"
     CDS             complement(1734..2087)
                     /locus_tag="PWN45_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDL0622019.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2540..3379)
                     /gene="noc"
                     /locus_tag="PWN45_000219"
     CDS             complement(2540..3379)
                     /gene="noc"
                     /locus_tag="PWN45_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDL0622020.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3422..4141)
                     /gene="rsmG"
                     /locus_tag="PWN45_000220"
     CDS             complement(3422..4141)
                     /gene="rsmG"
                     /locus_tag="PWN45_000220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL0622021.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4141..6018)
                     /gene="mnmG"
                     /locus_tag="PWN45_000221"
     CDS             complement(4141..6018)
                     /gene="mnmG"
                     /locus_tag="PWN45_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL0622022.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6085..7464)
                     /gene="mnmE"
                     /locus_tag="PWN45_000222"
     CDS             complement(6085..7464)
                     /gene="mnmE"
                     /locus_tag="PWN45_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL0622023.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7603..7956)
                     /gene="rnpA"
                     /locus_tag="PWN45_000223"
     CDS             complement(7603..7956)
                     /gene="rnpA"
                     /locus_tag="PWN45_000223"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL0622024.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8077..8214)
                     /gene="rpmH"
                     /locus_tag="PWN45_000224"
     CDS             complement(8077..8214)
                     /gene="rpmH"
                     /locus_tag="PWN45_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL0622025.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            8945..10306
                     /gene="dnaA"
                     /locus_tag="PWN45_000225"
     CDS             8945..10306
                     /gene="dnaA"
                     /locus_tag="PWN45_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL0622026.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10584..11717
                     /gene="dnaN"
                     /locus_tag="PWN45_000226"
     CDS             10584..11717
                     /gene="dnaN"
                     /locus_tag="PWN45_000226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL0622027.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            12098..12343
                     /gene="yaaA"
                     /locus_tag="PWN45_000227"
     CDS             12098..12343
                     /gene="yaaA"
                     /locus_tag="PWN45_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL0622028.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            12340..13452
                     /gene="recF"
                     /locus_tag="PWN45_000228"
     CDS             12340..13452
                     /gene="recF"
                     /locus_tag="PWN45_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL0622029.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            13462..15396
                     /gene="gyrB"
                     /locus_tag="PWN45_000229"
     CDS             13462..15396
                     /gene="gyrB"
                     /locus_tag="PWN45_000229"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL0622030.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            15433..18096
                     /gene="gyrA"
                     /locus_tag="PWN45_000230"
     CDS             15433..18096
                     /gene="gyrA"
                     /locus_tag="PWN45_000230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL0622031.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(18183..18995)
                     /locus_tag="PWN45_000231"
     CDS             complement(18183..18995)
                     /locus_tag="PWN45_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL0622032.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            19321..20835
                     /gene="hutH"
                     /locus_tag="PWN45_000232"
     CDS             19321..20835
                     /gene="hutH"
                     /locus_tag="PWN45_000232"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDL0622033.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    20908..21124
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21214..22500
                     /gene="serS"
                     /locus_tag="PWN45_000233"
     CDS             21214..22500
                     /gene="serS"
                     /locus_tag="PWN45_000233"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL0622034.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            23150..23845
                     /locus_tag="PWN45_000234"
     CDS             23150..23845
                     /locus_tag="PWN45_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0622035.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            23842..24171
                     /locus_tag="PWN45_000235"
     CDS             23842..24171
                     /locus_tag="PWN45_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0622036.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     regulatory      24376..24474
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            24534..25502
                     /locus_tag="PWN45_000236"
     CDS             24534..25502
                     /locus_tag="PWN45_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0622037.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            25793..26731
                     /locus_tag="PWN45_000237"
     CDS             25793..26731
                     /locus_tag="PWN45_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDL0622038.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            26746..28713
                     /gene="gdpP"
                     /locus_tag="PWN45_000238"
     CDS             26746..28713
                     /gene="gdpP"
                     /locus_tag="PWN45_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDL0622039.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            28710..29156
                     /gene="rplI"
                     /locus_tag="PWN45_000239"
     CDS             28710..29156
                     /gene="rplI"
                     /locus_tag="PWN45_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL0622040.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            29188..30588
                     /gene="dnaB"
                     /locus_tag="PWN45_000240"
     CDS             29188..30588
                     /gene="dnaB"
                     /locus_tag="PWN45_000240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL0622041.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            30866..32149
                     /locus_tag="PWN45_000241"
     CDS             30866..32149
                     /locus_tag="PWN45_000241"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL0622042.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            32579..32653
                     /locus_tag="PWN45_000242"
     tRNA            32579..32653
                     /locus_tag="PWN45_000242"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32612..32614,aa:Glu,seq:ttc)
     gene            32661..32733
                     /locus_tag="PWN45_000243"
     tRNA            32661..32733
                     /locus_tag="PWN45_000243"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32694..32696,aa:Asp,seq:gtc)
     gene            33353..34054
                     /locus_tag="PWN45_000244"
     CDS             33353..34054
                     /locus_tag="PWN45_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0622043.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            34067..35893
                     /gene="walK"
                     /locus_tag="PWN45_000245"
     CDS             34067..35893
                     /gene="walK"
                     /locus_tag="PWN45_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL0622044.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            35886..37220
                     /locus_tag="PWN45_000246"
     CDS             35886..37220
                     /locus_tag="PWN45_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622045.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            37221..38009
                     /locus_tag="PWN45_000247"
     CDS             37221..38009
                     /locus_tag="PWN45_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL0622046.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            38399..39199
                     /locus_tag="PWN45_000248"
     CDS             38399..39199
                     /locus_tag="PWN45_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0622047.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            39427..41745
                     /gene="adsA"
                     /locus_tag="PWN45_000249"
     CDS             39427..41745
                     /gene="adsA"
                     /locus_tag="PWN45_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDL0622048.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            42113..42592
                     /gene="rlmH"
                     /locus_tag="PWN45_000250"
     CDS             42113..42592
                     /gene="rlmH"
                     /locus_tag="PWN45_000250"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL0622049.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            42914..>43989
                     /locus_tag="PWN45_000251"
     CDS             42914..>43989
                     /locus_tag="PWN45_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDL0622050.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFA"
BASE COUNT        15296 a         7058 c         8099 g        13536 t
ORIGIN      
        1 ttacgtaata aaagctcgtg aattaaattg tagtgtattt gtttagaata tcctcttttt
       61 tagttatgaa tttgttacaa atattaagtg caaaagcaca cggaggtttt ctatatgaat
      121 aacggtacag taaaatggtt taacgcagaa aaaggttttg gtttcatcga acaagaaaat
      181 ggcggagacg tattcgtaca tttctcaggt atcgctagcg atggctacaa aactttagaa
      241 gaaggtcaaa aagttacttt cgaaatcact gaaggtcaac gtggagacca agcagttaac
      301 gtacaaactg tttaatctta caacataaaa cgactcatta taaatgaatc gctttgatta
      361 ccaacaaggt tctaaatctt gttggtattt ttttatgcta ttttggataa tataaataat
      421 atgagacgat accacgatga tttataaaaa cttatttgcc aaatacacac taaggaaaat
      481 tatgaggctg agtataccaa ttactgatat aatagaaagc gcatttgtca tcgtatttct
      541 agctttagtc gtttgtactt cactaggatc ttttccattc ataaaagcaa caattcgatc
      601 gtagttgtcc attttatgca ctttttttaa atcttctatt ttcatagatg cataaaaacc
      661 tattcccaaa atgattgcca ccgttacacc ccaagtccaa ttccaataaa caactaaagg
      721 tcccattaaa attgcagcta atgtcatgaa aataagcatc acatatgtcc ataagttaag
      781 acttcgttgc gctttaatat caggtacaaa tggaatggtc ccttttacta aatcatctaa
      841 agttacattg aacaattcac acatcattaa taagttatgt atgtctggta agcttttgtc
      901 attttcccaa ttagaaatac tctgcctaga tacatataac ttttcagcaa gatattcttg
      961 tgaataacca tctcgttccc tatacttttt aatttgttta ctcagattca attacatacg
     1021 cctccagtca attagtacaa tcattacaat cagactatca gataaagtta aatcaagtca
     1081 cctttatgat tgactaaatg ttttgacata cgacaacatc ggggttcgaa gactccctta
     1141 agacagacct gtatgattac gcgcttgtaa ttttctcaat gccaacttga atttcactta
     1201 ttaatgatgc aacttttcca tgctattcaa tgcttgcaaa gttagtccta gttaagagat
     1261 tacgttcaca ctatttacta ttcgattttc cagctttcca actatgcttt tctaatcttt
     1321 tttgtgctat aaattctacc gcttcaaaaa tacatactgc actgatgaac cagcttagga
     1381 aaccaacaac aatgctaatc aaccacatgt gcgtcatatg taacatcatc catgtaacta
     1441 aaatacaaac taatacccct gtcataccaa acactgcgcc acgacttaca tgcacagcga
     1501 ctttatcacc atattgcata cctgtaataa acatagacac taaaaatact gccggaaacg
     1561 ttgcaaatat accgccaaat tctttccaag gtagtgttac agatacaata taacttaata
     1621 atactgcaaa tccccccaca aaaaatttca ttaatgttaa tttcatgatg ttctgcctcc
     1681 aataatttat tacttaataa ctgaatacaa ctaattatca cgcattttcc aaactataaa
     1741 tgtctaatac tgaatattgc gagggaaatg acaaaccaac aaacaactgt gaatattgcg
     1801 cctttccgat aacctttatg cttaatgtag agagatgtca aaaatacagt taatatacaa
     1861 gacagaatac cgacaattgc tccagtacta agattcatcg acatctccac taattgcgta
     1921 ccacgatgat ctaaagctaa tgcgataata gccgcaagaa agactgccgg catagtagct
     1981 ataatacctc ctaacttacc acctacctta tcagcaataa ttgaagctaa tgcaacagcg
     2041 ataccaccaa tgacaaaatg aagaattgca cttccaattg aaaacatctt caacgtacct
     2101 ttctataatt aattcaatac aaaacgtatc attcacacaa agtgtaaaac ttaaattttc
     2161 tactgtaaag gcaactcaag cgatgttcat aaagctttca tgatactttc ataaactagc
     2221 aataaagggt tcaaatgaaa gtatatccag ctcaaattag ggagtgggac agaaatgata
     2281 ttttcgcaaa atttatttcg tcgtcccacc ccagcaaggt tgactagaat tgaaaaaagc
     2341 ttgttacaag cgcattttcg ttcagtcaac tactgccaat ataacttcgt agagcataga
     2401 acattgattt atgtaccagc ctgatcaaca tataaatata aatttttatg tttcacgtaa
     2461 aacaaaaaaa aagacaaagc tgttatgatc ttagctttgt cttttatgtg ttagtcatca
     2521 tgtatacgac atcctactac taacgtttat atattcgaat ttttatttca taataatctt
     2581 catgatcttt gtctttatgc tcaacatgta atcctgtttg ttgaatcgct tggatacttt
     2641 tgcctacctc atctcgtgct tgagtgacat cttgtgcaaa gcgaaggttt tgtgctttga
     2701 ctttttcggg ccccgttttt tggcgtactc tatcttcagt ctgtttcaca tttagctttt
     2761 gtgcaatgac ttgctcaatc aacgcttctt gttcgctatc agacaatgat aataccgctc
     2821 tcgcatgacg ttcagtaatt ttaccttctc ttaagcgaag tagtactttc ggcgccaact
     2881 tcaataaacg caacttattt gcaataaagc tttgactttt acctaaactt tttgccaatt
     2941 cactttgcgt tgtatcacca atttccaata atttcttata ggcttccgct tcttcaacaa
     3001 cagacaaatt ttctctttga atattctcaa ttaatgcaac aacagccgtc tcttcatcat
     3061 ccatatcacg aataataacg tctgcttgag gtaaatttag tgattgtatt gctctaaatc
     3121 ggcgctctcc agcaataatt tcaaacatat cttcttcaat cggtcttaca acaataggtt
     3181 gtagtaaacc atgttcatgt attgattcag caagttcttt aattttattt ggttcaaaca
     3241 cctgtcttgg ttgataacgg ttgggaacga tacgttcaat ttgaatggat tcaacattac
     3301 tattgcgatc ttcttcaata tgtccaatga tgtcatcttt gtttttcaaa ccaaataatt
     3361 ttgaaaaagg ttttttcatt atccattcgc tccttttatg ttagttgtaa aggattatgc
     3421 attatttttc aagtaaagga gtcttattag gcgtccctgg ttttcttgga tactttttcg
     3481 gcgtctgtct ttttttatca ataatgaaca tctggcgctc tccagcatct tctggcaatt
     3541 caaaggtatg tgtttctgta acattaccac ctaacacact aattgcaaat tttgcttctt
     3601 ctaattcttc ttcacctttt gaagatttta atgcaacaaa ctgtccacct tttttaacta
     3661 gcggtaaaca caattcactt aacacggata atctagctac tgctcttgca gtaacaacat
     3721 cataagactc cctgtagaca cccttaccaa atgtttctgc tctatcgtgt ataaagctga
     3781 catcctgtaa ttgtaattct gacgctaaat ggtttaaaaa ttgaatacgc ttatttaatg
     3841 aatcaacaat cgtcactttt aactgcggaa acattatttt taacggaata cttggaaaac
     3901 cagctccagc gcctacatca catatactta taggctgatt aaaatcaaaa taaaaactag
     3961 gtgcaatgga atcataaaaa tgtttcaaat atacatcgtg ttcatctgta atacttgtca
     4021 aattcatctt ttcattccat tcaacaagta aacgataata tgtttgaaac tgttgttttt
     4081 gagtctcagt taattgaata ttatgttctt ttaattgttc tgctaaccat tctacagtca
     4141 ttagtcactc accctttgga gtttaccttg ttctaagtaa atcaataata tagatatgtc
     4201 tgctggattt acccctgata ttctagaagc ttgtgcaata tttaaaggtt ttacttctga
     4261 taatttttct cgcgcttcag tcgccaaact atcaatctta ctataatcta agtcttctgg
     4321 aattttcttc tcttccatac gcttaacttt ctcaacttgt tgtagtgatt tattgatata
     4381 accttcatat tttgtttgta tttctacttg ttcttcaaca tctgcattca attgatgttc
     4441 ttcttctaaa agttctaaaa ttatatcgta agtcatttca ggtctgcgta ataaatcgat
     4501 agctaaaata ccatctttta agcgagaacc accatgttgt tcaataatcg cttgcgtatg
     4561 ttcgtttggt ttaatacgaa tatctgataa acgcttaatt tccgcatcaa tttgctgacg
     4621 tttttcatta aaacgtgcat atctttcttc agaaatcata ccaagttcat atcccatatc
     4681 cgtcaatctc aaatcagcat tatcatgacg tagtaacaaa cgatattctg cacgtgatgt
     4741 tagtaaacga taaggttcat tagtaccttt agttacaaga tcatcgatta agacaccaat
     4801 atatgcatct gaacgactta atatcttttc gcctgtgttt aacactttac ctgcagcgtt
     4861 aatacctgcc atcaatcctt gtcctgctgc ttcttcataa ccagatgtac cattaatttg
     4921 acctgcagta tataagtttt taatcatttt cgtttcaagt gtaggccata actgcgttgg
     4981 cacaatcgca tcatattcaa ttgcgtagcc ggcacgcatc atatctgctt tttcaagacc
     5041 tggtatcgtc tctaacattt gacgttgcac atgttcagga agacttgtag acaatccttg
     5101 cacatatact tcatttgtat tacgaccttc aggctctaag aaaagttgat gtcgcggctt
     5161 atcattaaat cgaacaaatt tatcttcaat tgaagggcaa taacgtggcc cggttccttt
     5221 aatcatccct gaatacattg cagatagatg taaattatca tcgataactt tgtgtgtttc
     5281 agcattagta tacgttagcc aacatggcaa ttgatctaat atatattctg ttgtttcaaa
     5341 gctgaatgca cgacctacat cgtcacctgg ttgtatttca gtcttcgaat agtcaattgt
     5401 ttttgaattt acacgcggtg gtgtacctgt tttaaaacga acaatatcaa aaccaagttc
     5461 tcttaaattg tctgataatg tgattgatgg taattggtga tttggtccac ttgaatactt
     5521 catattacct aaaatgattt caccacgtaa aaatgttccc gttgtaataa ttactgcttt
     5581 agataaatac tctgtaccaa tatttgtacg tacaccttta acttcattat cttctataat
     5641 aagttcgtct accatacctt gcattatatg caaattttct tcatcttcaa tcacgcgttt
     5701 catttcttgt tgataaagta ctttatctgc ttgcgctctt agtgctctta cagcaggtcc
     5761 tttacctgta tttaacattc tcatttgaat gtgtgtttta tcgattgttt ttgccatttg
     5821 tccacctaaa gcatcaattt cacgaacaac gataccttta gctggtccac ctacagatgg
     5881 gttacatggc ataaatgcaa tattatctaa atttattgtt agcattaatg ttttagcacc
     5941 acgtcttgca gatgctaaac ctgcttctac acctgcatgt cccgcaccta taacgattac
     6001 atcatattct tgaaccacaa tataaacctc cttatttgat atcttactag ccttcttaag
     6061 acggtattcc gtctatttca attactattt acctaagcag aattgactga ataactgatc
     6121 gatgagttca tcacttgcag tctcaccaat aatttctcct aatatttccc aagttctagt
     6181 taaatcaatt tgtaccatat ccataggcac accagattct gctgcatcaa tcgcatcttg
     6241 tatcgtttgt cttgcttgtt ttaataatga aatatgtctt gaattagaaa cataagtcat
     6301 atcttgattt tgtacttctc caccaaagaa caaatctcga atttgtattt ctaattcatc
     6361 aataccttct tgttttaaca ttgaagtttg aattaatggc gtatcaccta tcatatcttt
     6421 aacttcatta atatctatgt tttgctctaa atccatttta ttaacaatta cgattacatc
     6481 ttcattttta accacttcat ataatgtgta atcttcttga gtcaatgctt cgttattgtt
     6541 taatacaaat aaaattaagt ctgcttggct aagagccttt ctagagcgtt caacaccaat
     6601 cttctctact atatcttctg tctcacgtat accagcagta tcaactaatc ttaatggcac
     6661 gccacgaaca ttgacgtact cttctaagac atctctagta gtacctgcta cctcagttac
     6721 aatcgcttta ttatcttgta ttaaattatt taacatcgat gatttaccta cgtttggttt
     6781 accaacaata actgtagata aaccttcacg cataatttta ccctgcgcac cggtatctaa
     6841 taaacgatta atttcctgtt tgatttcttt agactgctct aaaagaaatt cagtagtcgc
     6901 atcttcaaca tcatcgtatt caggataatc aatattcact tccacttgag cgagtatctc
     6961 taatatagat tgacgttgtt ttttgattaa gtcacttaga cgaccttcaa tttgattcat
     7021 cgcaacttta gaagctctat ctgtcttcga gcgaataaag tccataactg cttcagcttg
     7081 agataaatca atacgaccat ttaaaaaggc acgttttgta aattcacctg gctcagccat
     7141 tctagcgcca tatgtcatag taagttccag cactctatta atcgttaaaa taccaccatg
     7201 acaattaatt tctataatat cttcgcgtgt aaatgttttt ggcgctctta acacagacac
     7261 cataacttct tcaatcactt ctttagactc tggatcaata atatgaccgt aattaatcgt
     7321 atgtgatgga acatcattta aaagatgttt tcctttatat aatttgtcag caatttcaac
     7381 ggcttgcggt ccagacaatc gaacaattcc aattgcccct tcacccattg gtgttgaaat
     7441 actcgtaatt gtatctaaat ccatattgct actcgcctcc ttcaacgatg tgaatacatt
     7501 ttaaagtaag ttattataac cctaaggtca gtcttacgtt tgtctgaggt aagacttcgg
     7561 gatgtgttga gtggttaatg ttttccttcc cctaccctat ccttacttaa tctttttatt
     7621 aaaaactttg gcaattttaa gtacgtgctc aagactattc tgtatttgta aagtcgtcat
     7681 atctttagct ggctgtcttg ctattacaat aatatctttg gccaatatat gcgacttatg
     7741 tactttgaaa ttttcacgta ttgctctttt aatcttgttt cttaacactg cattacctag
     7801 ttttttagaa acactaatac ctaagcgaaa atggtctatt tctttattat tacaagtgta
     7861 tacaacaaat tgtctgttgg ctacagaatg acctttttta tatattctct gaaaatctgc
     7921 attcttttta attcggtaag ctttttccaa taacatcact cgcttattta tcgtttttat
     7981 ttgaagctat atttaaactt ctattgagct tataacataa atttctattt attcttaatt
     8041 taaacgaaaa aaaagatcac tgatgagtca gtgatcttat gcagataaaa ctttacggcc
     8101 tttacgacga cggcgcgcta aaactttacg gccatttttt gtgctcatgc gttttctgaa
     8161 accatgaact ttactatgtt tacgtttatt tggttgataa gtacgtttta ccatgcaaaa
     8221 cacctccatc tttatacagt tatctaatat accagattat gcagactctg atcactttaa
     8281 tagataagat tttgcgtttc ttagattaaa attctcgtat tagctcattg attatctagt
     8341 cataattcaa gcaactacta caatataaca aaatcctatt tataacgcaa gttcatttta
     8401 tactactgct caattttttt acttttatcg attaaagata gaaatacacg atgcgagcaa
     8461 tcaaatttca taacatcacc atgagtttgg tccgaagcat gagtgtttac aatgttcgaa
     8521 caccttatac agttcttata catactttat aaattatttc ccaaactgtt ttgatacact
     8581 cactaacaga tactctatag aaggaaaagt tatccactta tgcacattta tagttttcag
     8641 aattgtggat aattagaaat tacacacaaa gttatactat ttttagcaac atattcacag
     8701 gtatttgaca tatagaggac tgaaaaagta taattgtgtg gataagtcgt ccaactcatg
     8761 attttataag gatttattta ttgattttta cataaaaata ctgtgcataa ctaataagca
     8821 agataaagtt atccaccgat tgttattaac ttgtggataa ttattaacat ggtgtgttta
     8881 gaagttatcc acggctgtta tttttgtgta taacttaaaa atttaagaaa gatggagtaa
     8941 atttatgtcg gaaaaagaaa tttgggaaaa agtgcttgaa attgctcaag aaaaattatc
     9001 agctgtaagt tactcaactt tcctaaaaga tactgagctt tacacgatta aagatggtga
     9061 agctatcgta ttatcgagta ttccttttaa tgcaaattgg ttaaatcaac aatatgctga
     9121 aattatccaa gcaatcttat ttgatgttgt aggctatgaa gttaaacctc actttattac
     9181 tactgaagaa ttagcaaatt atagtaataa tgaaactgct actccaaaag aaacaacaaa
     9241 accttctact gaaacaactg aggataatca tgtgcttggt agagagcaat tcaatgccca
     9301 taacacattt gacacttttg taatcggacc cggtaaccgc tttccacatg cagcgagttt
     9361 agctgtggcc gaagcaccag ccaaagcgta caatccatta tttatctatg gaggtgttgg
     9421 tttaggaaaa acccatttaa tgcatgccat tggtcatcat gttttagata ataatccaga
     9481 tgccaaagtg atttacacat caagtgaaaa attcacaaat gaatttatta aatcaattcg
     9541 tgataacgaa ggtgaagctt tcagagaaag atatcgtaat atcgacgtct tattaatcga
     9601 tgatattcag ttcatacaaa acaaggtaca aacacaagaa gaatttttct atacttttaa
     9661 tgaattgcat cagaataaca agcaaatagt tatttcgagt gatcgaccac caaaggaaat
     9721 tgcacaatta gaagaccgat tacgttcacg ctttgaatgg gggctaattg ttgatattac
     9781 gccaccagat tatgaaactc gaatggcaat tttgcagaag aaaattgaag aagaaaaatt
     9841 agatattcca ccagaagctt taaattatat agcaaatcaa attcaatcta atattcgtga
     9901 attagaaggt gcattaacac gtttacttgc atattcacaa ttattaggaa aaccaattac
     9961 aactgaatta actgctgaag ctttaaaaga tatcattcaa gcaccaaaat ctaaaaagat
    10021 taccatccaa gatattcaaa aaattgtagg ccagtactat aatgttagaa ttgaagattt
    10081 cagtgcaaaa aaacgtacaa agtcaattgc atatccgcgt caaatagcta tgtacttgtc
    10141 tagagagctt acagatttct cattacctaa aattggtgaa gaatttggtg ggcgtgatca
    10201 tacgaccgtc attcatgctc atgaaaaaat atctaaagat ttaaaagaag atcctatttt
    10261 taaacaagaa gtagagaatc ttgaaaaaga aataagaaat gtataagtag gaaactttgg
    10321 gaaatgtaat ctgttatata acagcactaa tgataacaat cattttttac atttctatat
    10381 gctaatgtgg caagatgagc aaaactcatt ttgtggataa tgtttaaaag tcatacacac
    10441 catacacaag ttatcaacat gtgtataact tcgccaaatc tatgttttta agacttatcc
    10501 accaatccac agcacctact actattacta agaacttaaa acctatataa ttatatataa
    10561 acgactggaa ggagttttaa ttaatgatgg aattcactat taaaagagat tattttatta
    10621 cacaattaaa tgacacatta aaagctattt caccaagaac aacattacct atattaactg
    10681 gtatcaaaat cgatgcgaaa gaacatgaag ttatattaac tggttcagac tctgaaattt
    10741 caatagaaat cactattcct aaaactgtag atggcgaaga tattgtcaat atttcagaaa
    10801 caggctcagt agtacttcct ggacgattct ttgttgatat tataaaaaaa ttacctggta
    10861 aagatgttaa attatctaca aatgaacaat tccagacatt aattacatca ggtcattctg
    10921 aatttaattt aagtggctta gatccagatc aatatccttt attacctcaa gtttctagag
    10981 atgacgcaat tcaattgtcg gtaaaagtgc ttaaaaacgt gattgcacaa acaaattttg
    11041 cagtgtccac ctcagaaaca cgcccagtac taactggtgt gaactggctt atacaagaaa
    11101 atgaattaat atgcacagcg actgactcac accgcttggc tgtaagaaag ttgcagttag
    11161 aagatgtttc tgaaaacaaa aatgtcatca ttccaggtaa ggctttagct gaattaaata
    11221 aaattatgtc tgacaatgaa gaagacattg atatcttctt tgcttcaaac caagttttat
    11281 ttaaagttgg aaatgtgaac tttatttctc gattattaga aggacattat cctgatacaa
    11341 cacgtttatt ccctgaaaac tatgaaatta aattaagtat agacaatggg gagttttatc
    11401 atgcgattga tcgtgcctct ttattagcgc gtgaaggtgg taataacgtt attaaattaa
    11461 gtacaggtga tgacgttgtt gaattgtctt ctacatcacc agaaattggt actgtaaaag
    11521 aagaagttga tgcaaacgat gttgaaggtg gtagcctgaa aatttcattc aactctaaat
    11581 atatgatgga tgctttaaaa gcaatcgata atgatgaggt tgaagttgaa ttcttcggta
    11641 caatgaaacc atttattcta aaaccaaaag gtgacgactc ggtaacgcaa ttaattttac
    11701 caatcagaac ttactaaaaa taaatataaa taaaggatga cgtgattaat taaaacgtca
    11761 tcctttattt tttggcaaaa ataattctag gtgcgtatgt aaaataaatt tggcagcatt
    11821 ttaaacagca aataaaagac gccaattaaa tttatgacaa atgtatccaa aatttaataa
    11881 gtgtgcttat atgcccttta aatttaaaat tttaatagtc aataacaagt tgaatataaa
    11941 agttaaacgc cgttaaatag cgttaaaaaa ttgaaaatga cagtattgcc aaaaaataag
    12001 aattaattat ttatatgtaa acggtttcta cctctatttt aaatgaaatt tgtgacaaaa
    12061 aaaggtataa tatattaatg acatacaaag aaatggagtg attattttgg ttcaagaagt
    12121 tgtagtagaa ggagacatta atttaggtca atttctaaaa acagaaggga ttattgaatc
    12181 tggtggtcaa gcaaaatggt tcttgcaaga cgttgaagta ttaattaatg gagtgcgtga
    12241 aacacgtcgc ggtaaaaagt tagaacatca agatcgtata gatatcccag aattacctga
    12301 agatgctggt tctttcttaa tcattcatca aggtgaacaa tgaagttaaa tacactccaa
    12361 ttagaaaatt atcgtaacta tgatgaggtt acgttgaaat gtcatcctga cgtgaatatc
    12421 ctcattggag aaaatgcaca aggaaagaca aatttacttg aatcaattta taccttagct
    12481 ttagcaaaaa gtcatagaac gagtaatgat aaggaactca tacgttttaa tgctgattat
    12541 gctaaaatag aaggtgagct tagttataga cacggcacga tgccattaac aatgtttata
    12601 actaaaaaag gtaaacaagt caaagtgaat cacttagagc aaagtcgtct aactcaatat
    12661 attggacacc tcaatgtggt tctatttgcg ccagaagatt tgaatattgt aaaaggctct
    12721 cctcaaataa gacgacgctt tatagatatg gagttgggcc aaatttctgc tgtttactta
    12781 aatgatttag ctcaatacca acgtatttta aagcaaaaga ataattactt aaagcagtta
    12841 caattaggcc aaaaaaagga cttaacaatg ttggaagtat taaatcagca gtttgctgaa
    12901 tatgcaatga aagtaactga taaacgtgca cattttattc aagagctaga gtcgttagct
    12961 aaaccgattc atgctggtat cacaaatgat aaagaagcgt tgtcgctgaa ttatttacct
    13021 agtcttaaat ttgattatgc tcaaaatgaa gcggcacgac ttgaagaaat tatgtctatt
    13081 cttagcgata atatgcaaag agaaaaagaa cgaggcatta gcttattcgg accacatcga
    13141 gatgatataa gttttgatgt gaatggcatg gatgctcaaa catatggttc tcaaggacag
    13201 caacgtacaa cggctttgtc cattaaatta gctgaaattg agttaatgaa tatcgaagtt
    13261 ggggaatatc ccatcttatt attagacgat gtactcagtg aattagatga ttcgcgtcaa
    13321 acgcatttat taagtacgat tcagcataaa gtacaaacat ttgtcactac gacatctgta
    13381 gatggtattg atcatgaaat catgaataac gctaaattgt atcgtattaa tcaaggtgaa
    13441 attataaagt aacagaaagc gatggtgact gcattgtcag atgtaaacaa cacggataat
    13501 tatggtgctg ggcaaataca agtattagaa ggtttagaag cagtacgtaa aagaccaggt
    13561 atgtatatag gatcgacttc agagagaggt ttgcaccatt tagtgtggga aattgtcgat
    13621 aatagtatcg atgaagcatt agctggttat gcaaatcaaa ttgaagttgt tattgaaaaa
    13681 gataactgga ttaaagtaac ggataacgga cgtggtatcc cagttgatat tcaagaaaaa
    13741 atgggacgtc cagctgtcga agttatttta actgttttac atgctggtgg taaatttggc
    13801 ggtggcggat acaaagtatc tggtggttta catggtgttg gttcatcagt tgtaaacgca
    13861 ttgtcacaag acttagaagt atatgtacac agaaatgaga ctatatatca tcaagcatat
    13921 aaaaaaggtg tacctcaatt tgacttaaaa gaagttggca caactgataa gacaggtact
    13981 gtcattcgtt ttaaagcaga tggagaaatc ttcacagaga caactgtata caactatgaa
    14041 acattacagc agcgtattag agagcttgct ttcttaaaca aaggaattca aatcacatta
    14101 agagatgaac gtgatgaaga aaacgttaga gaagactcct atcactatga gggcggtatt
    14161 aaatcgtacg ttgagttatt gaacgaaaat aaagaaccta ttcatgatga gccaatttat
    14221 attcatcaat ctaaagatga tattgaagta gaaattgcga ttcaatataa ctcaggatat
    14281 gccacaaatc ttttaactta cgcaaataac attcatacgt atgaaggtgg tacgcatgaa
    14341 gacggattca aacgtgcatt aacgcgtgtc ttaaatagtt atggtttaag tagcaagatt
    14401 atgaaagaag aaaaagatag actttctggt gaagatacac gtgaaggtat gacagcaatt
    14461 atatctatca aacatggtga tcctcaattc gaaggtcaaa cgaagacaaa attaggtaat
    14521 tctgaagtgc gtcaagttgt agataaatta ttctcagagc actttgaacg atttttatat
    14581 gaaaatccac aagtcgcacg tacagtggtt gaaaaaggta ttatggcggc acgtgcacgt
    14641 gttgctgcga aaaaagcgcg tgaagtaaca cgtcgtaaat cagcgttaga tgtagcaagc
    14701 cttccaggta aattagccga ttgctctagt aaaagtcctg aagaatgtga gattttctta
    14761 gtcgaagggg actctgccgg ggggtctaca aaatctggtc gtgactctag aacgcaggcg
    14821 attttaccat tacgaggtaa gatattaaat gttgaaaaag cacgattaga tagaattttg
    14881 aataacaatg aaattcgtca aatgatcaca gcatttggta caggaatcgg tggcgacttt
    14941 gatctagcga aagcaagata tcacaaaatc gtcattatga ctgatgccga tgtggatgga
    15001 gcgcatatta gaacattgtt attaacattc ttctatcgat ttatgagacc gttaattgaa
    15061 gcaggctatg tgtatattgc acagccaccg ttgtataaac tgacacaagg taaacaaaag
    15121 tattatgtat acaatgatag ggaacttgat aaacttaaat ctgaattgaa tccaacacca
    15181 aaatggtcta ttgcacgata caaaggtctt ggagaaatga atgcagatca attatgggaa
    15241 acaacaatga accctgagca ccgcgctctt ttacaagtaa aacttgaaga tgcgattgaa
    15301 gcggaccaaa catttgaaat gttaatgggt gacgttgtag aaaaccgtag acaatttata
    15361 gaagataatg cagtttatgc aaacttagac ttctaagcgc tgtgaactga acttttgaag
    15421 gaggaactct tgatggctga attacctcaa tcaagaataa atgaacgaaa tattaccagt
    15481 gaaatgcgtg aatcattttt agattatgcg atgagtgtta tcgttgctcg tgcattgcca
    15541 gatgttcgtg acggtttaaa accagtacat cgtcgtatac tatatggatt aaatgaacaa
    15601 ggtatgacac cggataaatc atataaaaaa tcagcacgta tcgttggtga cgtaatgggt
    15661 aaatatcacc ctcatggtga cttatctatt tatgaagcaa tggtacgtat ggctcaagat
    15721 ttcagttatc gttatccgct tgttgatggc caaggtaact ttggttcaat ggatggagat
    15781 ggcgcagcag caatgcgtta tactgaagcg cgtatgacta aaatcacact tgaactgtta
    15841 cgtgatatta ataaagatac aatagatttt atcgataact atgatggtaa tgaaagagag
    15901 ccgtcagtct tacctgctcg attccctaac ttattagcca atggtgcatc aggtatcgcg
    15961 gtaggtatgg caacgaatat tccaccacat aacttaacag aattaatcaa tggtgtactt
    16021 agcttaagta agaaccctga tatttcaatt gctgagttaa tggaggatat tgaaggtcct
    16081 gatttcccaa ctgctggact tattttaggt aagagtggta ttagacgtgc atatgaaaca
    16141 ggtcgtggtt caattcaaat gcgttctcgt gcagttattg aagaacgtgg aggcggacgt
    16201 caacgtattg ttgtcactga aattcctttc caagtgaata aggctcgtat gattgaaaaa
    16261 attgcagagc tcgttcgtga caagaaaatt gacggtatca ctgatttacg tgatgaaaca
    16321 agtttacgta ctggtgtgcg tgtcgttatt gatgtgcgta aggatgcaaa tgctagtgtc
    16381 attttaaata acttatacaa acaaacacct cttcaaacat catttggtgt gaatatgatt
    16441 gcacttgtaa atggtagacc gaagcttatt aatttaaaag aagcgttggt acattattta
    16501 gagcatcaaa agacagttgt tagaagacgt acgcaataca acttacgtaa agctaaagat
    16561 cgtgcccaca ttttagaagg attacgtatc gcacttgacc atatcgatga aattatttca
    16621 acgattcgtg agtcagatac agataaagtt gcaatggaaa gcttgcaaca acgcttcaaa
    16681 ctttctgaaa aacaagctca agctatttta gacatgcgtt taagacgtct aacaggttta
    16741 gagagagaca aaattgaagc tgaatataat gagttattaa attatattag tgaattagaa
    16801 gcaatcttag ctgatgaaga agtgttatta cagttagtta gagatgaatt gactgaaatt
    16861 agagatcgtt tcggtgatga tcgtcgtaca gaaattcaat taggtggatt tgaaaactta
    16921 gaggacgaag acttaattcc agaagaacaa atagtaatta cactaagcca taataactac
    16981 attaaacgtt tgccggtatc tacatatcgt gctcaaaacc gtggtggtcg tggtgttcaa
    17041 ggtatgaata cattggaaga agattttgtc agtcaattgg taactttaag tacacatgac
    17101 catgtattgt tctttactaa caaaggtcgt gtatacaaac ttaaaggtta cgaagtgcct
    17161 gagttatcaa gacagtctaa aggtattcct gtagtgaatg ctattgaact tgaaaatgat
    17221 gaagtcatta gtacaatgat tgctgttaaa gaccttgaaa gtgaagacaa cttcttagtg
    17281 tttgcaacta aacgtggtgt cgttaaacgt tcagcattaa gtaacttctc aagaataaat
    17341 agaaatggta agattgcgat ttcgttcaga gaagatgatg agttaattgc agttcgctta
    17401 acaagtggtc aagaagatat cttgattggt acatcacatg catcattaat tcgattccct
    17461 gaatcaacat tacgtccttt aggccgtaca gcaacgggtg tgaaaggtat tacacttcgt
    17521 gaaggtgacg aagttgtagg gcttgatgta gctcatgcaa acagtgttga tgaagtatta
    17581 gtagttactg aaaatggtta tggtaaacgt acgccagtta atgactatcg cttatcaaat
    17641 cgtggtggta aaggtattaa aacagctacg attactgagc gtaatggtaa tgttgtatgt
    17701 atcactacag taactggtga agaagattta atgattgtta ctaatgcagg tgtcattatt
    17761 cgactagatg ttgcagatat ttctcaaaat ggtcgtgcag cacaaggtgt tcgcttaatt
    17821 cgcttaggtg atgatcaatt tgtttcaacg gttgctaaag taaaagaaga tgcagaagat
    17881 gaaacgaatg aagatgagca atctacttca actgtatctg aagatggtac tgaacaacaa
    17941 cgtgaagcgg ttgtaaatga tgaaacacca ggaaatgcaa ttcatactga agtgattgat
    18001 tcagaagaaa atgatgaaga tggacgtatt gaagtaagac aagatttcat ggatcgtgtt
    18061 gaagaagata tacaacaatc atcagatgaa gaataataaa aaataagact tccctatatg
    18121 taggggagtc ttatttttat gctagaaagt aatgctttac tatattcaat gattagtaat
    18181 gactaacttt ctaattgttt cattgcgtaa ggtatttcat tgataagtct tgatggtggc
    18241 accacataca tatcttttgc aaggttttcg ccaataaaac tatgtgtata tgtggcactc
    18301 ataaccgctt cttttaagtt atcaaattga ccgacaaagc ttgtaatcat accagcaagt
    18361 gtatcgccca taccaccagt cgccattgct gggctaccga ttgtcaattt aaagtcttca
    18421 tctttaaaga aaatttcagt accatgtttt ttaagtacaa cagttgcacc taaacgatca
    18481 actgcttcac gattacgctc atatgtctgt tcctcaatag gaataccact taatcgttcc
    18541 cattctttga ggtgtggtgt aaagatcaca cgacatgtag gtaattgcgg tttcagttta
    18601 ctaaagattg taatcgcatc gccgtctacg attaaatttt gatgcggttg tatattttgt
    18661 agtaggaatg taatggcatt atttcctttg aaatcaacgc caagacctgg accaattagt
    18721 atactgtcag tcatttcaat cattttcgtc aacattttcg tatcattaat atcaataacc
    18781 atcgcttctg ggcaacgaga atgtaatgct gaatgatttg ttggatgtgt agctacagtg
    18841 attaaaccac taccgctaaa tacacatgca cgagccgcta acataatggc accacctaag
    18901 ttagcagatc caccaattaa taaaattttg ccataatcac ctttatgtga atcttcttta
    18961 cgcttaggaa tgttaataga atttaacgtt tccatagtga tataacctcc catgtaaaag
    19021 ccttttccga atttattcaa ttttaaaaat atatagtaac ttttaacaaa atgtattata
    19081 aatttctgaa ttcattatta tttgtcgtta aatacaatag aaaatactat acctgtatat
    19141 gcaattcgtc aatagataaa ttattaaata tgcttacaac aatcttaata tcctttaacg
    19201 cactacaata gtgctctgat aataggttat aaatgtacgt aaaaccattg tttcaataaa
    19261 aatgaaaacg tatacttcaa gaaggatggg ttacttaata taaacaaggg ggtaacatat
    19321 atgactttat atttagatgg tgaaacacta acaattgagg atattaaatc atttttacaa
    19381 caacaatcaa agattgaaat tattgatgat gcgttagaac gtgtcaaaaa aagtagagcg
    19441 gtagttgaac gtattattga aaatgaggaa acggtttacg gtattactac aggttttggg
    19501 ttatttagtg atgtacgtat agacccgacg caatataatg aattacaagt gaatctgata
    19561 cgctcacatg cctgtggact aggtgagcca ttttcaaaag aagtagcatt agtcatgatg
    19621 attttacgat tgaatacatt attaaaaggt cattcaggtg ccactttaga attagtgaga
    19681 caattacaat tttttataaa tgaacgtatt ataccgataa tcccacagca aggctctctc
    19741 ggtgcatcag gagatttagc gccattatca catttagcat tagcattaat tggtgaaggg
    19801 aaagtattgt acagagggga agaaaaggat agtgacgatg tattaagaga attaaataga
    19861 caacctttga accttcaggc taaagaaggt ttagcattga ttaatggtac gcaagctatg
    19921 acagctcaag gtgtcattag ttatatagaa gcagaagatt taggttacca atctgaatgg
    19981 attgctgcat taacgcatca gtctcttaat ggcattatag atgcatatcg acatgatgtg
    20041 cacgcagttc gtaattttca agaacagatt aatgtggcag cgcgtatgcg tgattggtta
    20101 gaaggatcaa cattaacgac gcgacaatca gaaatacgtg tacaagatgc atatacgttg
    20161 cgttgtatac cacaaatcca tggcgcgagt tttcaagtat tcaattatgt taaacagcaa
    20221 ttagaatttg aaatgaatgc ggctaatgat aatccactta tatttgagga agcaaatgaa
    20281 acgtttgtta tttcaggtgg taacttccat ggacaaccta ttgcttttgc attagatcat
    20341 cttaaattag gtgtaagtga attagcaaac gtatcggaac gtcgtctaga gcgactagta
    20401 aatcctcaat taaatggtga tttaccagca tttcttagtc cagagccagg attgcaaagt
    20461 ggcgcgatga ttatgcaata tgctgctgca agtctcgttt ctgaaaataa aactttagcg
    20521 catccagcga gtgttgattc tatcacttca tctgcgaacc aagaagatca cgtatctatg
    20581 ggaactacag ctgctagaca tggttatcaa attattgaaa atgcaagacg tgtgctggca
    20641 atcgaatgtg ttattgcatt acaagcagca gagttaaaag gtgttgaagg attatcacca
    20701 aaaacacgtc gcaagtatga tgagtttcga agtatcgtgc catccattac acatgatcgt
    20761 caatttcata aagatattga agcggttgca cagtatttaa agcaatcaat ttatcaaacg
    20821 actgcatgtc actaaatcga catgagtgat gtttgaaatc gttacttgaa aaagcaaata
    20881 tagtttgcta tattaaaaat aacttaataa gacattgttc gtaggacaag taatatatag
    20941 tgttcgatat cagagagctt gtggttagtg tgaacaagaa tcaacatata tatgaatcta
    21001 cctacttaat ttaaaagaac aatcggtgat aaccgttatt ttagtgaagt gcaatttagg
    21061 tttagtgtat ctttataact taaattgtta aatagggtgg caacgcgtag accacgtccc
    21121 ttgtagggat gtggtctttt tttattttct aaaaaataaa gacgtacgtc attccataat
    21181 aaatgataat tatttgggaa aggatgaagg ttaatgttag acattagatt attcagaaat
    21241 gagcctgaca cagttaagag caaaattgaa ttacgtggag atgatccaaa agttgtagat
    21301 gaaattttag aattggatga gcaacgacgt aaattaatta gtgcaacaga agaaatgaaa
    21361 gcacgtcgta ataaagtaag cgaagaaatc gcattaaaaa aacgtaataa agaaaatgct
    21421 gatgatgtga ttgctgaaat gcgcacatta ggtgacgata ttaaagaaaa agatagtcaa
    21481 ttaaatgaaa ttgataataa aatgacaggt atcctttgtc gtattccaaa tttaataagt
    21541 gatgatgtac ctcaaggtga atctgatgaa gataacgttg aagttaaaaa gtggggtaca
    21601 ccacgtgagt tttcatttga acccaaagca cattgggata ttgtagaaga attgaaaatg
    21661 gctgattttg atcgtgcagc aaaagtttca ggtgcgcgtt ttgtatattt aacaaatgaa
    21721 ggtgcgcaat tagagcgtgc tttaatgaac tatatgatta caaaacatac aacacaacat
    21781 ggttatacag aaatgatggt accacagctt gtgaacgcag atacaatgta tggtacaggt
    21841 caattaccta aatttgaaga agatttattt aaagtagaaa aagaaggatt atatacaatt
    21901 ccaactgctg aagtaccatt aacgaatttc taccgtaatg aaattattca accaggtgta
    21961 cttcctgaaa aattcactgg tcaatctgca tgtttccgta gtgaagcagg atcagcaggt
    22021 agagatacaa gaggattaat tcgtttacat caattcgata aagtggaaat ggtacgtttt
    22081 gaacaacctg aagattcatg gaatgcttta gaagaaatga caacaaacgc agaagcaatt
    22141 ctagaagagt taggtttacc ataccgtcgt gttattttat gtacaggtga tattggattt
    22201 agtgcaagca aaacatatga tttagaagtt tggttaccaa gctacaatga ttataaagaa
    22261 attagttcat gctcaaactg tacggatttc caagcgcgtc gtgctaacat ccgcttcaag
    22321 cgtgacaaag cagctaaacc agaattagca catacattaa atggtagtgg tttagcagtt
    22381 ggacgtacat ttgctgctat tgttgaaaat taccaaaatg aagatggaac agtaacaatt
    22441 ccagaagcat tagtaccatt tatgggtggt aaaacacaaa tttcaaaacc agttaaataa
    22501 aggctttagc tacaagcttt aaaaagtata tatctacgta tacttaaagc aagggcaaga
    22561 tactttaaat aatattttaa aaagtggtga cgaagctgtc gccacttttt ttgtgctgta
    22621 aaagtataat agtgagccaa tgcacataac aacaataaat taagtttgtg gtttaatggg
    22681 gtgaacgcat ttcattatag caacaatacg ggataattat gatgaactaa aacaatctaa
    22741 aacgtaacaa gtttgagcat caccaatata ggaaaggaag cgataaaata ctgatttcgt
    22801 tgatatgtag tatgagttat atcgatggag tagggtaggg ggaggggatg attataaggg
    22861 agtggtacat gaatcaatat cccagactca tcatcagata taaaaattta taaaattgat
    22921 acttaaaaac aactacaaat ccatagaaaa tatggaggta gtcttaaata aaaaattgaa
    22981 aattctcaaa aataaaaagt taatatgaag ctgactaaag actccggtat gtctaacctc
    23041 agacaaactg atgtctaatg ttattgctta gggtatagaa ctgtattaga ctaggtatat
    23101 tattttttcg taattatata aatataaagt ggcaaaggag gtaattgaga tgacaacaca
    23161 tttaagtttt agacaaggcg tgcaagagtg tatcccaaca ttattgggtt atgccggtgt
    23221 tggtatttca tttggtattg tggcttcgtc tcaaaacttt agtattttag aaattgtctt
    23281 gttatgtctt gttatatatg ccggtgctgc gcaatttatt atgtgcgcgt tgtttatagc
    23341 aggtacacct atatcagcga ttgtactaac tgtatttatc gtaaattcaa gaatgttcct
    23401 tttaagtatg tcgcttgcac caaacttcaa gacatatggg ttttggaacc gtgttggatt
    23461 aggttcatta gtaactgacg aaacgtttgg cgtcgccatt acaccttatt taaaaggaga
    23521 agctatcaat gatcgttgga tgcatggtct taacatcaca gcatatttat tttgggcaat
    23581 ttcatgtgta gctggggctt tatttggcga atatatctca aatccgcaaa cgctagggtt
    23641 agattttgct atcacggcta tgtttatctt tttggccatt gcgcaatttg aatcaattac
    23701 taaatcgcga ttaagaattt acatagtact cattattgcc gtcatagtaa tgatgttatc
    23761 gctaagtatg tttatgcctt catatctagc aatattaatt gcagccacaa tttcagcagc
    23821 gttaggagtg atgatggaac aatgataact catatgaaca tgttaatact tattttattg
    23881 tgtggtatcg taacgctatt aattcgaatt atacctttta tcatgatttc aaaagtgcaa
    23941 ttgcctgatg tcgtggttcg atggctatca tttatcccaa tcacactatt tacggcactt
    24001 gtcattgaca gcattattca acagacgcct catggtgagg ggtatacatt aaacatccct
    24061 tacattatcg cgctcattcc gacggttatt ttatctataa tcacgcgtag tttaactatt
    24121 acaattatta gtgggattgt tatcatggca gcattacgat ttttctttta aaaataactg
    24181 aaaatcattg aacagatatt taaacttagg taaactgtta gtaatcagaa aattctgata
    24241 tacaaatcgt ctatgttaag attgggaatc gtgtatacgt aagcgatttg gctaacgcat
    24301 gacaatgata acaacatttt aaatgataaa agtaattcat cactgaatct caactaacac
    24361 ataacaattt catatttctt attgtgagaa gttgagggac ttggccctgt gatacttcag
    24421 caaccgactt tatagcacgg tgctaaaacc aacgagttac tcgaatgata agtataaaga
    24481 cttcttactt ttcaataggg tgagaagttt ttttgtttaa ggaggaaaga acaatgacaa
    24541 attacacagt agatacttta aatttagggg aatttattac agaatctggg gaagtcatag
    24601 ataacttgcg tttgagatat gagcatgtcg gttatcatgg acaaccatta gttgtagttt
    24661 gtcatgcatt aactggcaat catttaacat atggaacaga tgattatccg ggttggtggc
    24721 gagaaattat tgatggggga tatataccca ttcacgatta tcaattttta acatttgatg
    24781 ttattggtag tcctttcggt tcaagttcac ctttaaacga ccctcatttt cctaaaaaat
    24841 taacattaag agatatagtc agagcgaatg aacgaggtat acaagccctt ggttatgata
    24901 agattaatat tttaataggg ggaagtcttg gaggtatgca agcaatggaa ctactctaca
    24961 atcaacagtt tgaagtagat aaagccatta tccttgctgc aacaagtcga acatcatctt
    25021 atagtagagc tttcaatgaa attgcaaggc aagccattca tcttggtggt aaggaaggtc
    25081 taagtattgc acgccaatta ggttttttga catatcgatc atcaaaaagt tatgatgaac
    25141 gtttcacgcc ggatgaagta gtcgcatacc aacaacatca aggtaataaa tttaaagaac
    25201 attttgattt gaattgttat ctgacactgc tagatgtatt ggatagtcac aacattgacc
    25261 gaggtcgcac agacgtaacg catgttttta aaaatttaga aacaaaagtg ttaacgatgg
    25321 ggttcataga tgatttgcta tatccggatg atcaagttcg tgcattaggt gaacgtttta
    25381 aatatcatcg tcatttcttc gtgcctgata atgttgggca tgatggattc ctactaaact
    25441 ttagtacctg ggcacctaac ttatatcatt tcttaaattt aaagcatttt aagcgtaagt
    25501 aataatatat attttcaaag aatgaagcca ctaaatcaca gttatcatat tcaaaaacat
    25561 ctagagtaca tgagagtgga aatgtattgt aaatctaaat caagcttgat tgctatgtag
    25621 tgggattaaa taatcatgtt gaaaatgaaa gcacacaaaa gcgattcaaa tgagtatgtt
    25681 aattacttat aaaacatgaa agttattatt caattaaatg aaatagaagc atacaatcta
    25741 taaattgtta attttcatta aagaggttaa aataatagct atagttaaaa atatgggagt
    25801 ggcttatgtg ttttcaaaaa tacaacctaa agcaacaata attgcaacga ttgcgttggt
    25861 atttgtcgct ttagctttat atctagtgcc tggtttagga ctaatatttg cattatttgc
    25921 aaccatacca ggtatcgttt tatggaataa atcaatacaa tctttcggga ttagtgcact
    25981 tattacagta attataacaa ctgttttagg taatactttc gttttaagtg ccatcatatt
    26041 agtcttaatt gcaagtttaa ttattggtca attgctcaaa gaaagaacgt ctaaagaaag
    26101 aatattatac gtaacaacag tagcgatgag cttaatttca ttaatcgctt ttatgttact
    26161 acaaacattc ggaaggattc caccatcagc gagcatagta aaacctttca agcaaacatt
    26221 acatgaagcg attacgatga gcggtgccga tgcgaatatg acccaaatat tagaagaagg
    26281 gtttagacaa gcgaccgttc aattaccagg tttcatcatt atcattacat ttttaatcgt
    26341 cttaattaac ttaatcgtta catttccgat tttacgaaaa tttaaaatcg ctacacctgt
    26401 atttaagcca cttttcgcgt ggcaaatgag cggtatttta ttatggatat acattattgt
    26461 tatcatatgt ttattattta caggtcaacc gagtgtgttc cagagcattc ttttaaactt
    26521 ccaacttgtg ttatcattag taatgtatat tcaaggttta agtgttattc atttctttgg
    26581 taaagcgaaa ggtttgccga atgcagtaac gattttacta ttggttatcg gtacaatact
    26641 gacacctacg acacatattg taggactact tggtgttatc gatttaagtt tgaatttgaa
    26701 gcgaatcatg aaaaataatt ctaaaaagtg aatagaggtg gaataatgaa tcggcagtcc
    26761 actaagaaag ctttactaat accatttgtc atcatgatca tcacagcaat tgttttaatg
    26821 ggtgtatggt ttatctttaa tagtcttata gcactaattg catctatcgt tcttgtcgtg
    26881 atgattattg ttagcatcat tttattcaga caagctttaa tgaaaatgga tagttatgta
    26941 gatggtttga gtgctcaaat ttcaacaaca aataataaag caatcaaaca tttaccaatt
    27001 ggtatcattg ttttagatga aaatgatcac atcgaatggg ttaaccaatt tatgacagat
    27061 catatggaag cgaatgtcat ttctgaatct gtaaatgaag tatttccaaa cattttaaag
    27121 caattagata gagtgaaatc cgttgaaata gaatataatc agtatcattt ccaagtacgt
    27181 tattctgaga atgatcactg cctctatttc tttgatataa ctgaacaagt acaaacaaat
    27241 gaactatatg aaaattctaa accaatcatt gcgacattat ttttagataa ctacgatgag
    27301 attacgcaaa atatgaatga tacgcagcgt tcggaaatca actcaatggt aacgcgtgtc
    27361 attagtcgat gggcaactga gtataatata tttttcaaaa gatacagttc cgatcaattc
    27421 gtagcctatt taaatcaaaa aatattagct gacttagaag aatctaaatt tgatatcttg
    27481 agtcaattac gtgaaaaaag tgttggttat cgtgcccaat taacattaag tatcggtgtt
    27541 ggtgaaggta ctgaaaattt aatcgactta ggtgaattat cacaatcagg cctagactta
    27601 gcattaggac gcggtggcga ccaagttgca attaaaagta ttaatggtaa tgtgcgtttc
    27661 tatggcggta agactgaccc gatggagaaa cgtactcgtg taagagcacg tgtgatctca
    27721 catgcgttaa aagatatcct tgcagagggt gacaaagtca ttatcatggg acataaacgt
    27781 cctgacttag atgcaattgg tgcagcaatc ggtgtgtcta gatttgcaat gatgaataat
    27841 ttagaagcat acatcgtatt aaatgagact gacattgatc caacattacg acgcgtgatg
    27901 aacgaaatag ataaaaagcc agagttaaga gagcgattta ttacatcaga tgatgcttgg
    27961 gatatgatga catctaagac aaccgtagtg attgttgata cgcataaacc ggaactggtt
    28021 ttagatgaaa atgtcttaaa taaagcaaac cgtaaagttg ttatcgatca tcatagacgt
    28081 ggtgaaagct tcatctctaa tccattgttg atatatatgg aaccatacgc aagttcgaca
    28141 gctgaattgg taacagagtt actggaatat caaccaacag aacaacgttt aacacgtctt
    28201 gaatcaacag tgatgtatgc aggtattatt gtagatacaa gaaactttac attacgaaca
    28261 ggatcaagaa cattcgatgc agcgagttat ttacgtgcac atggtgcaga tacgatttta
    28321 acgcaacatt tcttaaaaga tgatgtggat acttacatta atcgatctga attaattcga
    28381 actgtaaaag ttgaagataa tggcatagcc attgcgcatg gttcagacga taaaatttat
    28441 catccagtaa cagttgcaca agcagcagat gaactgttaa gtttagaagg tattgaagca
    28501 tcatatgttg ttgcgagacg tgaagataat ctgattggta tatctgcgcg ttcactcggt
    28561 tcagtaaatg tccagttaac aatggaagca cttggtggcg gtggacattt aaccaatgcg
    28621 gcaacacaac ttaaaggtgt gacagtcgaa gaggcgatag cacaattaca acaagcaatt
    28681 acagaacaat taagtaggag tgaagatgca tgaaagtaat ttttacacaa gatgttaaag
    28741 gtaaaggtaa aaaaggtgaa gttaaagaag taccagtagg ttatgcaaat aacttcttat
    28801 tgaaaaagaa ttatgctgta gaagcaacac caggtaacct taaacaatta gagttacaga
    28861 aaaaacgtgc aaaacaagaa cgccaacaag aaattgaaga tgctaaagca ttaaaagaaa
    28921 cgttatcaaa cattgaagtt gaagtatcag caaaaactgg tgaaggtggt aaattgtttg
    28981 ggtcagtaag tacaaaacaa attgccgaag cactaaaagc acaacatgat attaaaattg
    29041 ataaacgtaa aatggattta ccaaatggaa ttcattccct aggatatacg aatgtacctg
    29101 ttaaattaga taaagaagtt gaaggtacaa ttcgcgtaca cacagttgaa caataaagtt
    29161 ggattgaaat aagaggtgta accattcatg gatagaatgt atgagcaaaa tcaaatgccg
    29221 cataacaatg aagctgaaca gtctgtctta ggttcaatta ttatagatcc agaattgatt
    29281 aatactactc aggaagtttt gcttcctgag tcgttttata ggggtgccca tcaacatatt
    29341 ttccgtgcaa tgatgcactt aaatgaagat aataaagaaa ttgatgttgt aacattgatg
    29401 gatcaattat cgacggaagg tacgttgaat gaagcgggtg gcccgcaata tcttgcagag
    29461 ttatctacaa atgtaccaac gacgcgaaat gttcagtatt atactgatat cgtttctaag
    29521 catgcattaa aacgtagatt gattcaaact gcagatagta ttgccaatga tggatataat
    29581 gatgaacttg aactagatgc gattttaagt gatgcagaac gtcgaatttt agagctatca
    29641 tcttctcgtg aaagcgatgg ctttaaagac attcgagacg tcttaggaca agtgtatgaa
    29701 acagctgaag agcttgatca aaatagtggt caaacaccag gtatacctac aggatatcga
    29761 gatttagacc aaatgacagc agggttcaac cgaaatgatt taattatcct tgcagcgcgt
    29821 ccatctgtag gtaagactgc gttcgcactt aatattgcac aaaaagttgc aacgcatgaa
    29881 gatatgtata cagttggtat tttctcgcta gagatgggtg ctgatcagtt agccacacgt
    29941 atgatttgta gttctggaaa tgttgactca aaccgcttaa gaacgggtac tatgactgag
    30001 gaagattgga gtcgttttac tatagcggta ggtaaattat cacgtacgaa gatttttatt
    30061 gatgatacac cgggtattcg aattaatgat ttacgttcta aatgtcgtcg attaaagcaa
    30121 gaacatggct tagacatgat tgtgattgac tacttacagt tgattcaagg tagtggttca
    30181 cgtgcgtccg ataacagaca acaggaagtt tctgaaatct ctcgtacatt aaaagcatta
    30241 gcccgtgaat tagaatgtcc agttatcgca ttaagtcagt tatctcgtgg tgttgaacaa
    30301 cgacaagata aacgtccaat gatgagtgat attcgtgaat ctggttcgat tgagcaagat
    30361 gccgatatcg ttgcattctt ataccgtgat gattactata accgtggcgg cgatgaagat
    30421 gatgacgatg atggtggttt cgagccacaa acgaatgatg aaaacggtga aattgaaatt
    30481 atcattgcta agcaacgtaa cggtccaaca ggcacagtta agttacattt tatgaaacaa
    30541 tataataaat ttaccgatat cgattatgca catgcagata tgatgtaaaa aagtttttcc
    30601 gtacaataat cattaagatg ataaaattgt acggttttta ttttgttctg aacgggttga
    30661 tatatgttaa gtttgtgtat tgaaagtgat aaattagtac tgtcaacgcc tctgttaaag
    30721 ggtttttagg acgttgaaaa cgatttgtta aaatgatttt tcttttaaaa aggccgaaaa
    30781 tcaatgttcg atttttattt gcattatggt ctcgatattg gtagaatatc aaatggttaa
    30841 atgagaaaaa cttggaggtg ctcacatgtc atcaatcgta gtagttggga cacaatgggg
    30901 agacgaagga aaaggaaaaa taacggattt cttggcagaa cagtcagatg ttatcgcgcg
    30961 tttttcaggt ggtaataatg caggccatac cattcaattt ggcggagaaa catataaatt
    31021 acatttagta ccatctggta tcttttacaa agacaaatta gcggtaatcg gtaacggagt
    31081 cgttgttgat ccagttgcac tattgaaaga attagacgga ttaaatgaac gtggcattcc
    31141 tacaagtaat ttacgtatat ctaatcgtgc gcaagtgatt ttaccatatc acttagcaca
    31201 agatgaatat gaagaacgtt tacgtggtga caataagatt ggtacaacta aaaaaggtat
    31261 cggtccagca tatgtagaca aagttcaacg tatcggtatt cgtatggcag atttacttga
    31321 aaaagaaaca ttcgaaagat tattaaaatc aaacattgaa tataaacaag catatttcaa
    31381 aggtatgttt aacgaaacat gtccatcatt tgatgatatc tttgaagaat attatgcagc
    31441 aggtcaacgt ctaaaagaat ttgtaacaga cacatcaaaa atcttagacg atgcatttgt
    31501 agcagatgaa aaggtacttt tcgaaggtgc gcaaggtgta atgttagata tcgaccatgg
    31561 tacatatcca ttcgttacat caagtaatcc aattgcaggt aacgttactg ttggtacagg
    31621 tgtaggtcct acattcgttt caaaggtaat tggtgtatgt aaagcttata catcacgtgt
    31681 tggtgatggt ccattcccta ctgaattatt cgatgaagat ggacatcata ttagagaggt
    31741 tggtcgtgaa tacggtacaa caacaggacg tccacgtcgt gtaggttggt ttgattcagt
    31801 tgtattacgt cactctcgtc gtgtaagtgg tattacagat ttatctatta actcaatcga
    31861 tgttttaaca ggcctagaca cagtgaaaat ctgtacagct tatgaattag acggtaaaga
    31921 aattactgag tacccagcaa acttagatca attaaaacgt tgtaaaccaa tctttgaaga
    31981 gttaccaggt tggacagaag acgtaacaaa tgtgcgtact ttagaagaat tacctgaaaa
    32041 tgcacgtaaa tatttagagc gtatttcaga attatgtaat gtacaaattt ctatcttctc
    32101 agttggtcca gatagagaac aaacaaacct attaaaagaa ttgtggtaga actttatata
    32161 agtcatacac aatgattata aatacatgag ccttctatct ttattggtag gaggcttttg
    32221 ttatgcttgc ttctgtatcg attcgattat ttagataaaa aatactaacg taaaggcgat
    32281 atttgctagt cataatttag aagattagat gatatttaac gaaaattaag atgaaatact
    32341 tgaatgtaag aagtctgatg tcgaaaatag ctattaaaat agagtagacg taagtgtaaa
    32401 tgaaagcacc taaaatagaa aaatttcaaa aatagcgtaa ttattataat gaatagactg
    32461 ccaataaaat gcaatttttc acttataaca ttcttcaaaa aataatagca aaattatgta
    32521 aaaaatatct tgtcatggca agattggctg tgctataatc tatcttgtgc ttaagaacgg
    32581 ctccttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgagtccc
    32641 gtaggagtca ccatttttta ggtctcgtag tgtagcggtt aacacgcctg cctgtcacgc
    32701 aggagatcgc gggttcgatt cccgtcgaga ccgtacaaat gcctatccaa gaggataggc
    32761 atttttttgc gtttaatatt atattaataa aagatatatg gacgaatgat aatcatattg
    32821 atttatctgt tcgtccattt tctttaaaat gtatgaacct caagtaactt agtggttgga
    32881 tatgaaagat aaacgtagac aataaaatct ttattagacg tacaaacata tgctactgtc
    32941 aacatatttc ttcgttgtga tatgccacca gtcctccata acatcaattg ttaaagtaac
    33001 gaataacgaa taatgatatt tattttctga gcaatgacgt gcaactagaa gttgccatta
    33061 tcctaatttt attattggaa tagagacctc atcattgtgt taaatatcat tgtcacaatc
    33121 cgccgtgaga aactaataaa aaatagtaat atataagttt atattggaaa atagaattaa
    33181 tagcttataa atggtaaatt atataatagg ttactatacg ttataagacg gaaaatgcgc
    33241 acaataacaa aaatagtaag cgacatcctg tgatttttta cacaaacata aacgataaag
    33301 aacaaaaaat gataaaataa tattaatgat ttaagaaaag aggtttatgc aaatggctag
    33361 aaaagttgtt gtagttgatg atgaaaaacc gattgctgat attttagaat ttaacttaaa
    33421 aaaagaagga tacgatgtgt actgtgcata cgatggtaat gatgcagtcg acttaattta
    33481 tgaagaagaa ccagacatcg tattactaga tatcatgtta cctggtcgtg atggtatgga
    33541 agtatgtcgt gaagtgcgca aaaaatacga aatgccaata ataatgctta ctgctaaaga
    33601 ttcagaaatt gataaagtgc ttggtttaga actaggtgca gatgactatg taacgaaacc
    33661 gtttagtacg cgtgaattaa tcgcacgtgt gaaagcgaac ttacgtcgtc attactcaca
    33721 accagcacaa gacactggaa atgtaacgaa tgaaatcaca attaaagata ttgtgattta
    33781 tccagacgca tattctatta aaaaacgtgg cgaagatatt gaattaacac atcgtgaatt
    33841 tgaattgttc cattatttat caaaacatat gggacaagta atgacacgtg aacatttatt
    33901 acaaacagta tggggctatg attactttgg cgatgtacgt acggtcgatg taacgattcg
    33961 tcgtttacgt gaaaagattg aagatgatcc gtcacatcct gaatatattg tgacgcgtag
    34021 aggcgttgga tatttcctcc aacaacatga gtagaggtcg aaacgaatga agtggctaaa
    34081 acaactacaa tcccttcata ctaaacttgt aattgtttat gtattactga ttatcattgg
    34141 tatgcaaatt atcgggttat attttacaaa taaccttgaa aaagagctgc ttgataattt
    34201 taagaagaat attacgcagt acgcgaaaca attagaaatt agtattgaaa aagtatatga
    34261 cgaaaagggc tccgtaaatg cacaaaaaga tattcaaaat ttattaagtg agtatgccaa
    34321 ccgtcaagaa attggagaaa ttcgttttat agataaagac caaattatta ttgcgacgac
    34381 gaagcagtct aaccgtagtc taatcaatca aaaagcgaat gatagttctg tccaaaaagc
    34441 actatcacta ggacaatcaa acgatcattt aattttaaaa gattatggcg gtggtaagga
    34501 ccgtgtctgg gtatataata tcccagttaa agtcgataaa aaggtaattg gtaatattta
    34561 tatcgaatca aaaattaatg acgtttataa ccaattaaat aatataaatc aaatattcat
    34621 tgttggtaca gctatttcat tattaatcac agtcatccta ggattcttta tagcgcgaac
    34681 gattaccaaa ccaatcaccg atatgcgtaa ccagacggtc gaaatgtcca gaggtaacta
    34741 tacgcaacgt gtgaagattt atggtaatga tgaaattggc gaattagctt tagcatttaa
    34801 taacttgtct aaacgtgtac aagaagcgca ggctaatact gaaagtgaga aacgtagact
    34861 ggactcagtt atcacccata tgagtgatgg tattattgca acagaccgcc gtggacgtat
    34921 tcgtatcgtc aatgatatgg cactcaagat gcttggtatg gcgaaagaag acatcatcgg
    34981 atattacatg ttaagtgtat taagtcttga agatgaattt aaactggaag aaattcaaga
    35041 gaataatgat agtttcttat tagatttaaa tgaagaagaa ggtctaatcg cacgtgttaa
    35101 ctttagtacg attgtgcagg aaacaggatt tgtaactggt tatatcgctg tgttacatga
    35161 cgtaactgaa caacaacaag ttgaacgtga gcgtcgtgaa tttgttgcca atgtatcaca
    35221 tgagttacgt acacctttaa cttctatgaa tagttacatt gaagcacttg aagaaggtgc
    35281 atggaaagat gaggaacttg cgccacaatt tttatctgtt acccgtgaag aaacagaacg
    35341 aatgattcga ctggtcaatg acttgctaca gttatctaaa atggataatg agtctgatca
    35401 aatcaacaaa gaaattatcg actttaacat gttcattaat aaaattatta atcgacatga
    35461 aatgtctgcg aaagatacaa catttattcg agatattccg aaaaagacga ttttcacaga
    35521 atttgatcct gataaaatga cgcaagtatt tgataatgtc attacaaatg cgatgaaata
    35581 ttctagaggc gataaacgtg tcgagttcca cgtgaaacaa aatccacttt ataatcgaat
    35641 gacgattcgt attaaagata atggcattgg tattcctatc aataaagtcg ataagatatt
    35701 cgaccgattc tatcgtgtag ataaggcacg tacgcgtaaa atgggtggta ctggattagg
    35761 actagccatt tcgaaagaga ttgtggaagc gcacaatggt cgtatttggg caaacagtgt
    35821 agaaggtcaa ggtacatcta tctttatcac acttccatgt gaagtcattg aagacggtga
    35881 ttgggatgaa taataaggag catattaaat ctgtcatttt agcactactc gtcttgatga
    35941 gtgtcgtatt gacatatatg gtatggaact tttctcctga tattgcaaat gtcgacaata
    36001 cagatagtaa gaagagtgaa acgaaacctt taacgacacc tatgacagcc aaaatggata
    36061 caactattac gccatttcag attattcatt cgaaaaatga tcatccagaa ggaacgattg
    36121 cgacggtatc taatgtgaat aaactgacga aacctttgaa aaataaagaa gtgaagtccg
    36181 tggaacatgt tcgtcgtgat cataacttga tgattcctga tttgaacagt gattttatat
    36241 tattcgattt tacgtatgat ttaccgttat caacatatct tggtcaagta ctgaacatga
    36301 atgcgaaagt accaaatcat ttcaatttca atcgtttggt catagatcat gatgctgatg
    36361 ataatatcgt gctttatgct ataagcaaag atcgccacga ttacgtaaaa ttaacaacta
    36421 caacgaaaaa tgatcatttt ttagatgcat tagcagcagt gaaaaaagat atgcaaccat
    36481 acacagatat catcacaaac aaagatacaa ttgatcgtac gacgcatgtt tttgcaccaa
    36541 gtaaacctga aaagttaaaa acatatcgca tggtatttaa cacgattagt gttgagaaaa
    36601 tgaatgctat actatttgac gattcaacca tcgttcgtag ttcaaagagt ggtgttacaa
    36661 cctacaacaa taatacaggt gtcgcaaact ataacgataa aaatgaaaaa tatcattata
    36721 aaaacctgtc cgaagatgaa gcgagttcca gcaaaatgga agaaacgatt ccaggaacct
    36781 ttgattttat taatggtcat ggtggtttct taaacgaaga ctttagattg tttagtacga
    36841 ataatcagtc aggcgagtta acatatcaac gtttccttaa tggttatcca acgtttaata
    36901 aagaaggttc taatcaaatt caagtcactt ggggtgaaaa aggcgtcttt gactatcgtc
    36961 gttcgttatt acgcaccgac gttgttttaa atagtgagga taataaatcg ttgccgaaat
    37021 tagagtctgt acgttcaagc ttagcgaaca atagtgatat taattttgaa aaagtaacaa
    37081 acatcgctat cggttacgaa atgcaggata attcagatca taatcacatt gaagtgcaga
    37141 ttaacagtga actcgtaccg cgttggtatg tagaatatga tggcgaatgg tatgtttata
    37201 acgatgggag gcttgaataa atgaactgga aactgacaaa gacacttttc attttcgtgt
    37261 ttattcttgt caacatcgtg ttagtatcga tttatgttaa taaagtcaat cgctcacaca
    37321 ttaatgaagt cgagagtaac aatgaagtta attttcagca agaagaaatt aaagtaccga
    37381 ctagtatatt gaataaatca gttaaaggta taaaattaga gcaaattaca gggcgatcaa
    37441 aagactttag ttctaaagct aaaggcgatt cggatttgac cacatcagat ggtggaaaat
    37501 tattgaatgc gaacattagt caatcggtaa aggtcagtga caataactta aaagatttga
    37561 aagattatgt taacaagcgc gtatttaaag gtgctgaata tcaattaagc gagattagtt
    37621 cagattctgt aaaatatgaa caaacgtatg atgattttcc gattttaaat aacagtaaag
    37681 cgatgttgaa ctttaatata gaagataaca aagcgactag ttataaacaa tcaatgatgg
    37741 atgacattaa gcccacagat ggtgcagata agaagcatca agtgattggt gtgagaaaag
    37801 caatcgaggc attatattat aatcgttact tgaaaaaagg tgatgaagtc attaatgcta
    37861 gactcggtta ctactcagtc gtgaatgaaa cgaatgttca attgttacaa ccaaactggg
    37921 aaattaaagt gaagcatgac ggtaaggata aaacgaatac ttactatgtc gaagcgacaa
    37981 ataataaccc taaaattatt aatcattaat atgaatcgta ataagctagc attgcaagct
    38041 catcatatgt gagaagcggt gctagctttt ttgctggtac ggtttattat ggctgatgtt
    38101 tttgcgtctc caacgtgcgc atttattcat attttaagta gaaccgcatt gtaaaattag
    38161 tgtaactgtt attttgaaaa ctttagtatt tgtctaatca ttgttataat aattaagaaa
    38221 ttcattgcac gtgattatca aaatttaaat ataagaaacc ggtcgatgaa ctaaagttac
    38281 ataataggaa aggtatacaa aacagctaat atactgatag tttctgtagg gaaaatcgta
    38341 tatttgcact gatgtatatt gcagtcatat agagagattg actgtttaaa gagaaaggat
    38401 gagccgcttg atacgcatga gtgtattagc aagtggtagt acaggtaacg ccacttttgt
    38461 agaaaatgaa aaaggtagtc tattagttga tgttggtttg actggaaaga aaatggaaga
    38521 attgtttagt caaattgacc gtaatattca agatttaaat ggtattttag taacccatga
    38581 acatattgat catattaaag gattaggtgt tttggcgcgt aaatatcaat tgccaattta
    38641 tgcgaatgaa aagacttggc aggcaattga aaagaaagat agtcgcatcc ctatggatca
    38701 gaaattcatt tttaatcctt atgaaacaaa atctattgca ggtttcgatg ttgaatcgtt
    38761 taacgtgtca catgatgcaa tagatccgca attttatatt ttccataata actataagaa
    38821 gtttacgatt ttaacggata cgggttacgt gtctgatcgt atgaaaggta tgatacgtgg
    38881 cagcgatgcg tttatttttg agagtaatca tgacgtcgat atgttgagaa tgtgtcgtta
    38941 tccatggaag acgaaacaac gtattttagg cgatatgggt catgtatcta atgaggatgc
    39001 ggctcatgca atgacagacg tgattacagg taacacgaaa cgtatttacc tatcgcattt
    39061 atcacaagac aataacatga aagatttggc gcgtatgagt gttggccaag tattgaacga
    39121 acacgatatt gatacggaaa aagaagtatt gctatgtgat acggataaag ctattccaac
    39181 gccaatatat acaatataaa tgagagtcat ccgataaagt tccgcattgc tgtgagacga
    39241 ctttatcggg tgctttttta tgttgttggt gggaaatggc tgttgttgag ttgaatcggc
    39301 ttgattgaaa tgtgtaaaat aattcgatat taaatgtaat ttataaataa tttacataaa
    39361 atcaatcatt ttaatataag gattatgata atatattggt gtatgacagt taatggaggg
    39421 aacgaaatga aagctttatt acttaaaaca agtgtatggc tcgttttgct ttttagtgta
    39481 atgggattat ggcaagtctc gaacgcggct gagcagcata caccaatgaa agcacatgca
    39541 gtaacaacga tagacaaagc aacaacagat aagcaacaag taccgccaac aaaggaagcg
    39601 gctcatcatt ctggcaaaga agcggcaacc aacgtatcag catcagcgca gggaacagct
    39661 gatgatacaa acagcaaagt aacatccaac gcaccatcta acaaaccatc tacagtagtt
    39721 tcaacaaaag taaacgaaac acgcgacgta gatacacaac aagcctcaac acaaaaacca
    39781 actcacacag caacgttcaa attatcaaat gctaaaacag catcactttc accacgaatg
    39841 tttgctgcta atgcaccaca aacaacaaca cataaaatat tacatacaaa tgatatccat
    39901 ggccgactag ccgaagaaaa agggcgtgtc atcggtatgg ctaaattaaa aacagtaaaa
    39961 gaacaagaaa agcctgattt aatgttagac gcaggagacg ccttccaagg tttaccactt
    40021 tcaaaccagt ctaaaggtga agaaatggct aaagcaatga atgcagtagg ttatgatgct
    40081 atggcagtcg gtaaccatga atttgacttt ggatacgatc agttgaaaaa gttagagggt
    40141 atgttagact tcccgatgct aagtactaac gtttataaag atggaaaacg cgcgtttaag
    40201 ccttcaacga ttgtaacaaa aaatggtatt cgttatggaa ttattggtgt aacgacacca
    40261 gaaacaaaga cgaaaacaag acctgaaggc attaaaggcg ttgaatttag agatccatta
    40321 caaagtgtga cagcggaaat gatgcgtatt tataaagacg tagatacatt tgttgttata
    40381 tcacatttag gaattgatcc ttcaacacaa gaaacatggc gtggtgatta cttagtgaaa
    40441 caattaagtc aaaatccaca attgaagaaa cgtattacag ttattgatgg tcattcacat
    40501 acagtacttc aaaatggtca aatttataac aatgatgcat tggcacaaac aggtacagca
    40561 cttgcgaata tcggtaagat tacatttaat tatcgcaatg gagaggtatc gaatattaaa
    40621 ccgtcattga ttaatgttaa agacgttgaa aatgtaacac cgaacaaagc attagctgaa
    40681 caaattaatc aagctgatca aacatttaga gcacaaactg cagaggtaat tattccaaac
    40741 aataccattg atttcaaagg agaaagagat gacgttagaa cgcgtgaaac aaatttagga
    40801 aacgcgattg cagatgctat ggaagcgtat ggcgttaaga atttctctaa aaagactgac
    40861 tttgccgtga caaatggtgg aggtattcgt gcctctatcg caaaaggtaa ggtgacacgc
    40921 tatgatttaa tctcagtatt accatttgga aatacgattg cgcaaattga tgtaaaaggt
    40981 tcagacgtct ggacggcttt cgaacatagt ttaggcgcac caacaacaca aaaggacggt
    41041 aagacagtgt taacagcgaa tggcggttta ctacatatct ctgattcaat ccgtgtttac
    41101 tatgatataa ataaaccgtc tggcaaacga attaatgcta ttcaaatttt aaataaagag
    41161 acaggtaagt ttgaaaatat tgatttaaaa cgtgtatatc acgtaacgat gaatgacttc
    41221 acagcatcag gtggcgacgg atatagtatg ttcggtggtc ctagagaaga aggtatttca
    41281 ttagatcaag tactagcaag ttatttaaaa acagctaact tagctaagta tgatacgaca
    41341 gaaccacaac gtatgttatt aggtaaacca gcagtaagtg aacaaccagc taaaggacaa
    41401 caaggtagca aaggtagtaa gtctggtaaa gatacacaac caattggtga cgacaaagtg
    41461 atggatccag cgaaaaaacc agctccaggt aaagttgtat tgttgctagc gcatagagga
    41521 actgttagta gcggtacaga aggttctggt cgcacaatag aaggagctac tgtatcaagc
    41581 aagagtggga aacaattggc tagaatgtca gtgcctaaag gtagcgcgca tgagaaacag
    41641 ttaccaaaaa ctggaactaa tcaaagttca agcccagaag cgatgtttgt attattagca
    41701 ggtataggtt taatcgcgac tgtacgacgt agaaaagcta gctaaaatat attgaaaata
    41761 atactactgt atttcttaaa taagaggtac ggtagtgttt ttttatgaaa aaaagcgata
    41821 accgttgata aatatgggat ataaaaacga ggataagtaa taagacatca aggtgtttat
    41881 ccacagaaat ggggatagtt atccagaatt gtgtacaatt taaagagaaa tacccacaat
    41941 gcccacagag ttatccacaa atacacaggt tatacactaa aaatcgggca taaatgtcag
    42001 gaaaatatca aaaactgcaa aaaatattgg tataataaga gggaacagtg tgaacaagtt
    42061 aataacttgt ggataactgg aaagttgata acaatttgga ggaccaaacg acatgaaaat
    42121 caccatttta gctgtaggga aactaaaaga gaaatattgg aagcaagcca tagcagaata
    42181 tgaaaaacgt ttaggcccat acaccaagat agacatcata gaagttccag acgaaaaagc
    42241 accagaaaat atgagtgaca aagaaattga gcaagtaaaa gaaaaagaag gccaacgaat
    42301 actagccaaa atcaaaccac aatccacagt cattacatta gaaatacaag gaaagatgct
    42361 atcttccgaa ggattggccc aagaattgaa ccaacgcatg acccaagggc aaagcgactt
    42421 tgttttcgtc attggcggat caaacggcct gcacaaggac gtcttacaac gcagtaacta
    42481 cgcactatca ttcagcaaaa tgacattccc acatcaaatg atgcgggttg tgttaattga
    42541 acaagtgtac agagcattta agattatgcg aggagaagca tatcataaat gatgcggttt
    42601 tttcagccgc ttcataaagg gattttgaat gtatcagaac atatgaggtt tatgtgaatt
    42661 gctgttatgt ttttaagaag cttatcataa gtaatgaggt tcatgatttt tgacatagtt
    42721 agcctccgca gtctttcatt tcaagtaaat aatagcgaaa tattctttat actgaatact
    42781 tatagtgaag caaagttcta gctttgagaa aattctttct gcaactaaat atagtaaatt
    42841 acggtaaaat ataaataagt acatattgaa gaaaatgaga cataatatat tttataatag
    42901 gagggaattt caaatgatag acaactttat gcaggtcctt aaattaatta aagagaaacg
    42961 taccaataat gtagttaaaa aatctgattg ggataaaggt gatctatata aaactttagt
    43021 ccatgataag ttacccaagc agttaaaagt gcatataaaa gaagataaat attcagttgt
    43081 agggaaggtt gctactggga actatagtaa agttccttgg atttcaatat atgatgagaa
    43141 tataacaaaa gaaacaaagg atggatatta tttggtatat ctttttcatc cggaaggaga
    43201 aggcatatac ttatctttga atcaaggatg gtcaaagata agtgatatgt ttccgcggga
    43261 taaaaatgct gcaaaacaaa gagcattaac tttatcttcc gaactcaata aatatattac
    43321 atcaaatgaa tttaatactg gaagatttta ttacgcagaa aataaagatt catcttatga
    43381 tttaaaaaat gattatccat caggatattc tcatggatca ataagattca aatattatga
    43441 tttgaatgaa ggattcacag aagaagatat gctagaggat ttaaagaaat ttttagaact
    43501 atttaatgaa ttagcttcaa aagttacaaa aacatcctat gatagcttgg tcaatagcat
    43561 agacgaaata caggaagaca gcgaaattga agaaattaga acagcacaaa aagataagac
    43621 actcaaggaa gtggaagcac ctaaaggaat aattccaaaa tataaaaaag gtgtatcaaa
    43681 gactactaaa aatgattcag aaattgaaaa atcaaataaa gagaataaat taaccggtaa
    43741 agttggagaa aaattagcgc taaattactt taatgagcta attgataata aaatagacga
    43801 agataagaaa gaacagttta ggaatatttt aaatgataat ccaggctctc aacacggtca
    43861 tggctatgat ttagtagctt ttgatccaac aaatacagat aaagctgtag aaaaatttat
    43921 tgaaattaaa acatctacat cttctagtat tgaggaacca ttttttatgt cgctaaatga
    43981 aatgtttgc
//
LOCUS       DANJFZ010000005        43578 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.005, whole genome shotgun sequence.
ACCESSION   DANJFZ010000005 DANJFZ010000000
VERSION     DANJFZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.005"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            83..1474
                     /gene="norB"
                     /locus_tag="PWN45_000252"
     CDS             83..1474
                     /gene="norB"
                     /locus_tag="PWN45_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDL0622051.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            1872..33137
                     /gene="ebh"
                     /locus_tag="PWN45_000253"
     CDS             1872..33137
                     /gene="ebh"
                     /locus_tag="PWN45_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDL0622052.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALYQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(33196..33597)
                     /locus_tag="PWN45_000254"
     CDS             complement(33196..33597)
                     /locus_tag="PWN45_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL0622053.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(34138..34842)
                     /locus_tag="PWN45_000255"
     CDS             complement(34138..34842)
                     /locus_tag="PWN45_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDL0622054.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            35087..35281
                     /locus_tag="PWN45_000256"
     CDS             35087..35281
                     /locus_tag="PWN45_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDL0622055.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            35293..35544
                     /locus_tag="PWN45_000257"
     CDS             35293..35544
                     /locus_tag="PWN45_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDL0622056.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            35582..36706
                     /locus_tag="PWN45_000258"
     CDS             35582..36706
                     /locus_tag="PWN45_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDL0622057.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            36722..37159
                     /locus_tag="PWN45_000259"
     CDS             36722..37159
                     /locus_tag="PWN45_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDL0622058.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            37583..38539
                     /locus_tag="PWN45_000260"
     CDS             37583..38539
                     /locus_tag="PWN45_000260"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL0622059.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            38739..39218
                     /locus_tag="PWN45_000261"
     CDS             38739..39218
                     /locus_tag="PWN45_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0622060.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            39233..40072
                     /gene="fakB2"
                     /locus_tag="PWN45_000262"
     CDS             39233..40072
                     /gene="fakB2"
                     /locus_tag="PWN45_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDL0622061.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            40158..40691
                     /gene="msrA"
                     /locus_tag="PWN45_000263"
     CDS             40158..40691
                     /gene="msrA"
                     /locus_tag="PWN45_000263"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0622062.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            40684..41112
                     /gene="msrB"
                     /locus_tag="PWN45_000264"
     CDS             40684..41112
                     /gene="msrB"
                     /locus_tag="PWN45_000264"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL0622063.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            41124..41624
                     /locus_tag="PWN45_000265"
     CDS             41124..41624
                     /locus_tag="PWN45_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0622064.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            41624..41845
                     /locus_tag="PWN45_000266"
     CDS             41624..41845
                     /locus_tag="PWN45_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL0622065.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            42037..43527
                     /locus_tag="PWN45_000267"
     CDS             42037..43527
                     /locus_tag="PWN45_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDL0622066.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT        17186 a         7435 c         7814 g        11143 t
ORIGIN      
        1 accattcaat tattctatgg aacagacaaa tttctgttat ggaatttgtc tgtttttcaa
       61 aagtataggg aggcaaatag agatggaaaa gccgtcaaga gaggcatttg aaggcaataa
      121 taagttgtta ataggaattg ttctaagtgt aataacgttt tggctatttg cacaatcatt
      181 ggttaatgtt gtaccaatac ttgaagatag tttcaataca gatattggaa cggttaatat
      241 cgccgttagt ataactgctt tattttcagg aatgtttgta gtaggagcag gtggtcttgc
      301 tgataaatat ggcagaatta aactcacgaa cattggtatt atcttaaata tattaggttc
      361 attattaatc attatttcaa atattccttt attacttatt ataggaagat taattcaagg
      421 actttcagca gcatgtatta tgcctgcaac tttgtctatt attaagtcat attacattgg
      481 gaaagataga caacgcgctt taagttattg gtcaattggc tcatggggcg gctctggtgt
      541 ttgttcattt tttggaggtg cagttgcaac gcttttaggt tggcgttgga ttttcatcct
      601 atcaattata atttcattaa ttgcactgtt tcttattaaa ggcacacctg aaactaaatc
      661 taaatcgatt tctctaaata aatttgacat taaaggtctg gttcttttag tcattatgct
      721 cctcagttta aatattttaa ttactaaagg atcagaatta ggtgtaacct cacttctttt
      781 tattacttta ttagctattg caattggatc ttttagttta tttatagttc ttgaaaagcg
      841 tgctacaaat cctttaatcg attttaaatt atttaaaaat aaagcttaca caggtgcaac
      901 agcttcaaac tttttgttaa atggtgttgc aggaacatta atagtagcca acacatttgt
      961 tcaaagaggt ttaggatatt cttcattgca agcaggaagt ttatcaatca cttatttagt
     1021 aatggtacta attatgattc gtgttggtga aaagttactt caaacactcg gatgcaagaa
     1081 accaatgtta attggaacag gagttcttat tgtcggagaa tgtctcattt cattaacttt
     1141 cttgccagaa atattctatg tcatttgttg tattataggt tatttattct ttggtttagg
     1201 actagggata tatgctacac catcaacaga tacagcaatt gcaaatgcac cgttagaaaa
     1261 agtaggcgtt gctgcaggta tctataaaat ggcttctgca ttaggtggag catttggcgt
     1321 cgcattgagt ggtgcagtat atgcaatcgt atcaaatatg acaaacattt atacaggtgc
     1381 aatgattgca ttatggttaa atgcaggtat gggaatatta tcattcgtta tcattttgtt
     1441 acttgtgcct aaacaaaacg atactcaatt atgataattg agaattaaat tgaaatcata
     1501 caagtcgcta caatattaaa caaaaatata aaccgattct tatgtgtcat tattttaaat
     1561 gaacataggg attggttttt tattactctt ttacgctact ttatttataa ttattataaa
     1621 ttgtcacaaa ttcaatttac cttacaatat attttgtgtt attatattct ggagcataaa
     1681 taaattgttc aacacatagt tgtaatgtgt ttcaatactt tttggataga ttgcgaaatt
     1741 gtattgaatc gtcatcgttt taaattttta aatgagaatg gaatgagcat tacaatacac
     1801 aagcaatcaa aagtaaatac attcacaaca caacagagac ataacaacaa gataaggagt
     1861 gaacaatagc tgtgaattat cgtgataaaa ttcaaaagtt tagtattcgt aaatatacag
     1921 ttggtacatt ttcaactgtc attgcgacat tggtattttt aggattcaat acatcacaag
     1981 cacatgctgc tgaaacaaat caaccagcaa gcgtggttaa acagaaacaa caaagtaata
     2041 atgaacagac tgagaatcga gaatctcaag tacaaaattc tcaaaattca caaaatggtc
     2101 aatcattatc tgctactcat gaaaatgagc aaccaaatat tagtcaagct aatttagtag
     2161 atcaaaaagt agcgcaatca tctactacta atgatgaaca accagcatct caaaatgtaa
     2221 atacaaagaa agattcggca acggctgcga caacacaacc agataaagaa caaagtaagc
     2281 ataaacaaaa cgaaagtcaa tctgctaata aaaatggaaa cgacaataga gcggctcatg
     2341 tagaaaatca tgaagcaaat gtagtaacag cttcagattc atctgataat ggtaacgtac
     2401 aacatgaccg aaatgaatta caagcgtttt ttgatgcaaa ttatcatgat tatcgcttta
     2461 ttgaccgtga aaatgcagat tctggcacat ttaactatgt aaaaggcatt tttgataaga
     2521 ttaatacgtt attaggcagt aatgatccaa taaacaataa agacttgcaa cttgcataca
     2581 aagaattgga acaagctgtt gctttaattc gtacaatgcc tcaacgtcaa cagactagcc
     2641 gacgttcaaa tagaattcaa acgcgttcgg ttgagtcaag agctgcagag cctagatcag
     2701 tatcagacta tcaaaatgca aattcatcat attatgttga aaatgctaat gatggttcgg
     2761 gctatcctgt tggtacatat atcaatgctt ctagtaaagg ggcgccatat aatttaccaa
     2821 ctacaccatg gaatacattg aaggcctctg actcaaagga aattgctctt atgacagcga
     2881 aacaaactgg agacgggtac caatgggtta ttaagtttaa taaaggacat gctccacatc
     2941 aaaatatgat cttttggttt gcattaccag cagaccaagt gccagtagga agaactgact
     3001 ttgtaacagt taattcagat ggaacaaatg tacaatggag tcatggagca ggagcaggtg
     3061 caaataaacc acttcaacaa atgtgggaat atggagtaaa tgatcctcat cgttcacatg
     3121 actttaaaat aagaaataga agtggccaag taatatatga ctggccaact gtccatattt
     3181 attctttaga agatttatct agagcgagtg attattttag tgaagctgga gcgacacctg
     3241 ctactaaagc ttttggtaga caaaattttg aatatattaa tggtcaaaaa cctgctgaat
     3301 caccgggtgt tcctaaagtt tatactttca tcggtcaagg tgatgcaagt tatacaattt
     3361 catttaaaac acaaggtcca actgttaata aattgtacta tgcagcaggt gggcgtgctt
     3421 tagagtacaa tcaattattt atgtacagtc aactatacgt cgaatcaacg caagaccatc
     3481 aacaacgtct taatggttta agacaagtgg ttaatcgtac atatcgcata ggtacaacta
     3541 aacgtgtaga agtgagtcaa ggaaatgtac aaacgaaaaa ggtattagaa agtacaaacc
     3601 taaatataga tgattttgtt gatgatcctt taagttatgt taagacgccg agtaataaag
     3661 tgttaggatt ttattcgaat aatgcaaata ctaatgcttt tagaccgggt ggagcccaac
     3721 aattaaatga atatcaatta agtcaattat ttactgatca aaaattacaa gaagcagcaa
     3781 gaactagaaa cccaataaga ttaatgattg gtttcgacta tcctgatgct tatggtaata
     3841 gtgaaacttt agttcctgtt aacttaacgg tattacctga aatccaacat aatattaaat
     3901 tctttaaaaa tgacgatact caaaatattg ctgaaaaacc attttcaaaa caagctgggc
     3961 atccagtttt ctatgtatat gcaggtaacc aagggaatgc ttccgtgaat ttaggtggta
     4021 gcgtaacatc tattcaacca ttacgtatta atttaacaag taatgagaat tttacagata
     4081 aagattggca aattacaggt attccgcgta cattacacat tgaaaactcg acaaatagac
     4141 ctaataatgc cagagaacgc aatattgaac ttgttggtaa cttattacca ggggattact
     4201 ttggaacgat acgttttgga cgtaaagaac aattattcga aattcgtgtt aaaccacata
     4261 caccaacaat tacaacgaca gctgagcaat taagaggtac agcattacaa aaagtgcctg
     4321 ttaatatttc gggaataccg ttggatccat cggcattggt ttatttagtt gcaccaacaa
     4381 atcaaactac gaatggtggt agtgaggcag atcaaatacc atctggttat acgatacttg
     4441 cgactggtac acctgatggg gtgcataata caattactat acgaccgcaa gattatgttg
     4501 tattcatacc acctgtaggt aaacaaatta gagcagtagt ttattataat aaagtagttg
     4561 catctaatat gagtaatgct gttactattt tgccagatga cattccacca acaatcaata
     4621 atcctgttgg aataaatgcc aaatactatc gaggcgacga agtcaacttt acaatgggtg
     4681 tctctgatag acattctggt ataaaaaata caactattac gacattgccg aatggttgga
     4741 catcaaattt aacaaaagcg gataagaata atggctcatt atctattaca ggtagagtga
     4801 gtatgaatca ggcatttaac agtgatatta catttaaagt gtcagcgaca gacaatgtca
     4861 ataatacgac aaatgatagt caatctaaac atgtttcaat tcatgtaggt aaaattagtg
     4921 aagatgctca tccgattgta ttaggaaata ctgagaaagt tgtagtagtc aatccgactg
     4981 ctgtatctaa tgatgaaaag caaagcataa ttactgcctt tatgaataaa aaccaaaata
     5041 taagaggata tttagcatca actgatccag taactgtcga taataatggt aatgtcacat
     5101 tacattaccg tgatggctca tcgacaacgc ttgatgctac aaatgtgatg acatacgaac
     5161 cagttgtgaa acctgaatac caaactgtca atgctgctaa aacagcaacg gtaacgattg
     5221 ctaaaggaca atcatttagt attggtgata ttaaacaata ttttacttta agtaatggac
     5281 aacctattcc aagtggcaca tttacaaata ttacatctga tagaactatt ccaactgcac
     5341 aagaagttag tcaaatgaac gcaggcacgc agttatacca tataactgct acaaatgcgt
     5401 atcataaaga tagtgaagac ttctatatta gtttgaaaat catcgatgtg aaacaaccag
     5461 aaggcgatca acgtgtatat cgtacatcaa catatgattt aactactgat gaaatctcaa
     5521 aagtaaaaca agcatttatt aatgcaaata gagatgtaat tacgcttgcc gaaggtgata
     5581 tttcagttac aaatacacct aatggtgcta atgtaagtac tattacagta aatattaata
     5641 aaggtcgatt aacgaaatca ttcgcgtcaa acctagctaa tatgaatttc ttgcgttggg
     5701 ttaatttccc acaagattat acagtgacat ggacgaatgc aaaaattgca aacagaccaa
     5761 cagatggtgg tttatcatgg tctgatgacc ataaatcttt aatttatcgt tatgatgcta
     5821 cattaggtac tcaaattacg acgaatgata ttttaacaat gttaaaagca acaactacag
     5881 tgcctggatt gcgaaataac attactggta atgaaaaatc acaagcagaa gctggcggaa
     5941 gacctaactt tagaacgact ggttattcac aatcaaatgc gacaactgat ggtcaacgtc
     6001 aatttacgtt gaatggtcaa gtgattcaag tgttagacat catcaaccct tcaaacggtt
     6061 atggtgggca acctgttaca aattcaaata ctcgtgcaaa ccatagtaac tcaactgttg
     6121 ttaacgtaaa cgaaccggca gctaatggtg ctggcgcatt tacaattgac cacgttgtaa
     6181 aaagtaattc tacacataat gcaagtgatg cagtttataa agcacagtta tacttaacgc
     6241 catatggtcc aaaacaatat gttgaacatt taaatcaaaa tacaggaaat actactgacg
     6301 ctattaacat ttattttgta ccaagtgact tagtgaatcc aacaatttca gtaggtaatt
     6361 acactaatca tcaagtgttc tcaggtgaaa catttacaaa tactattaca gcgaatgata
     6421 actttggtgt gcaatctgta actgtaccaa atacatcaca aattacaggt actgttgata
     6481 ataaccatca acatgtttct gcaacggcac caaatgtgac atcagcaact aataagacaa
     6541 tcaatttatt agcaactgat acaagcggca atacagctac aacttcgttc aatgtaacag
     6601 tgaaaccttt gcgtgataaa tatcgagttg gtacttcatc aacggctgct aatcctgtga
     6661 gaattgccaa tatttcgaat aatgcgacag tatcacaagc tgatcaaacg acaattatta
     6721 attcgttaac gtttactgaa acagtaccaa atagaagtta tgcaagagca agtgcgaatg
     6781 aaatcactag taaaacagtt agtaatgtca gtcgtactgg aaataatgcc aatgtcacag
     6841 taactgttac ttatcaagat ggaacaacat caacagtgac tgtacctgta aagcatgtca
     6901 ttccagaaat cgttgcacat tcgcattaca ctgtacaagg ccaagacttc ccagcaggta
     6961 atggttctag tgcatcagat tactttaagt tatctaatgg tagtgacatt gcagatgcaa
     7021 ctattacatg ggtaagtgga caagcgccaa ataaagataa tacacgtatt ggtgaagata
     7081 taactgtaac tgcacatatc ttaattgatg gcgaaacaac gccgattacg aaaacagcaa
     7141 catataaagt agtaagaact gtaccgaaac atgtctttga aacagccaga ggtgttttat
     7201 acccaggtgt ttcagatatg tatgatgcga aacaatatgt taagccagta aataattctt
     7261 ggtcgacaaa tgcgcaacat atgaatttcc aatttgttgg aacatatggt cctaacaaag
     7321 atgttgtagg catatctact cgtcttatta gagtgacata tgataataga caaacagaag
     7381 atttaactat tttatctaaa gttaaacctg acccacctag aattgacgca aactctgtga
     7441 catataaagc aggtcttaca aaccaagaaa ttaaagttaa taacgtatta aataactcgt
     7501 cagtaaaatt atttaaagca gataatacac cattaaatgt cacaaatatt actcatggta
     7561 gcggttttag ttcggttgtg acagtaagtg acgcgttacc aaatggcgga attaaagcaa
     7621 aatcttcaat ttcaatgaac aatgtgacgt atacgacgca agacgaacat ggtcaagttg
     7681 ttacagtaac aagaaatgaa tctgttgatt caaatgacag tgcaacagta acagtgacac
     7741 cacaattaca agcaactact gaaggcgctg tatttattaa aggtggcgac ggttttgatt
     7801 tcggacacgt agaaagattt attcaaaacc cgccacatgg ggcaacggtt gcatggcatg
     7861 atagtccaga tacatggaag aatacagtcg gtaacactca taaaactgcg gttgtaacat
     7921 tacctaatgg tcaaggtacg cgtaatgttg aagttccagt caaagtttat ccagttgcta
     7981 atgcaaaggc gccatcacgt gatgtgaaag gtcaaaattt gactaatgga acggatgcga
     8041 tgaactacat tacatttgat ccaaatacaa acacaaatgg tatcactgca gcatgggcaa
     8101 atagacaaca accaaataac caacaagcag gcgtgcaaca tttaaatgtc gatgtcacat
     8161 atccaggtat ttcagctgct aaacgagttc ctgttactgt taatgtatat caatttgaat
     8221 tccctcaaac tacttatacg acaacggttg gaggcacttt agcaagtggt acgcaagcat
     8281 caggatatgc acatatgcaa aatgctactg gtttaccaac agatggattt acgtataaat
     8341 ggaatcgtga tactacaggt acaaatgacg caaactggtc agctatgaat aaaccgaatg
     8401 tggctaaagt cgttaacgca aaatatgacg tcatctataa cggacatact tttgcaacat
     8461 ctttaccagc gaaatttgta gtaaaagatg tgcaaccagc gaaaccaact gtgactgaaa
     8521 cagcggcagg agcgattaca attgcacctg gagcaaacca aacagtgaat acacatgccg
     8581 gtaacgtaac gacatacgct gataaattag ttattaaacg taatggtaac gttgtgacga
     8641 catttacacg tcgcaataat acgagtccat gggtgaaaga agcatctgca gcaactgtag
     8701 caggtattgc tggaactaat aatggtatta ctgttgcagc aggtactttc aaccctgctg
     8761 atacaattca agttgttgca acgcaaggaa gcggagagac agtgagtgat gagcaacgta
     8821 gtgatgattt cacagttgtc gcaccacaac cgaaccaagc gactactaag atttggcaaa
     8881 atggtcatat tgatatcacg cctaataatc catcaggaca tttaattaat ccaactcaag
     8941 caatggatat tgcttacact gaaaaagtgg gtaatggtgc agaacatagt aagacaatta
     9001 atgttgttcg tggtcaaaat aatcaatgga caattgcgaa taagcctgac tatgtaacgt
     9061 tagatgcaca aactggtaaa gtgacgttca atgccaatac tataaaacca aattcatcaa
     9121 tcacaattac tccgaaagca ggtacaggtc actcagtaag tagtaatcca agtacattaa
     9181 ctgcaccggc agctcatact gtcaacacaa ctgaaattgt gaaagattat ggttcaaatg
     9241 taacagcagc tgaaattaac aatgcagttc aagttgctaa taaacgtact gcaacgatta
     9301 aaaatggcac agcaatgcct actaatttag ctggtggtag cacaacgacg attcctgtga
     9361 cagtaactta caatgatggt agtactgaag aagtacaaga gtccattttc acaaaagcgg
     9421 ataaacgtga gttaatcaca gctaaaaatc atttagatga tccagtaagc actgaaggta
     9481 aaaagccagg tacaattacg cagtacaata atgcaatgca taatgcgcaa caacaaatca
     9541 atactgcgaa aacagaagca caacaagtga ttaataatga gcgtgcaaca ccacaacaag
     9601 tttctgacgc actaactaaa gttcgtgcag cacaaactaa gattgatcaa gctaaagcat
     9661 tacttcaaaa taaagaagat aatagccaat tagtaacgtc taaaaataac ttacaaagtt
     9721 ctgtgaacca agtaccatca actgctggta tgacgcaaca aagtattgat aactataatg
     9781 cgaagaagcg tgaagcagaa actgaaataa ctgcagctca acgtgttatt gacaatggcg
     9841 atgcaactgc acaacaaatt tcagatgaaa aacatcgtgt cgataacgca ttaacagcat
     9901 taaaccaagc gaaacatgat ttaactgcag atacacatgc cttagagcaa gcagtgcaac
     9961 aattgaatcg cacaggtaca acgactggta agaagccggc aagtattact gcttacaata
    10021 attcgattcg tgcacttcaa agtgacttaa caagtgctaa aaatagcgct aatgctatta
    10081 ttcaaaagcc aataagaaca gtacaagaag tgcaatctgc gttaacaaat gtaaatcgtg
    10141 tcaatgagcg attaacgcaa gcaattaatc aattagtacc tttagctgat aatagtgctt
    10201 taaaaactgc taagacgaaa cttgatgaag aaatcaataa atcagtaact actgatggta
    10261 tgacacaatc atcaatccaa gcatatgaaa atgctaaacg tgcgggtcaa acagaatcaa
    10321 caaatgcaca aaatgttatt aacaatggtg atgcgactga ccaacaaatt gccgcagaaa
    10381 aaacaaaagt agaagaaaaa tataatagct taaaacaagc aattgctgga ttaactccag
    10441 acttggcacc attacaaact gcaaaaactc agttgcaaaa tgatattgat cagccaacga
    10501 gtacgactgg tatgacaagc gcatctattg cagcatttaa tgaaaaactt tcagcagcta
    10561 gaactaaaat tcaagaaatt gatcgtgtat tagcctcaca tccagatgtt gcgacaatac
    10621 gtcaaaacgt gacagcagcg aatgccgcta aatcagcact tgatcaagca cgtaatggct
    10681 taacagtcga taaagcgcct ttagaaaatg cgaaaaatca actacaacat agtattgaca
    10741 cgcaaacaag tacaactggt atgacacaag actctataaa tgcatacaat gcgaagttaa
    10801 cagctgcacg taataagatt caacaaatca atcaagtatt agcaggttca ccgactgtag
    10861 aacaaattaa tacaaatacg tctacagcaa atcaagctaa atctgattta gatcatgcac
    10921 gtcaagcttt aacaccagat aaagcgccgc ttcaaactgc gaaaacgcaa ttagaacaaa
    10981 gcattaatca accaacggat acaacaggta tgacgaccgc ttcgttaaat gcgtacaacc
    11041 aaaaattaca agcagcgcgt caaaagttaa ctgaaattaa tcaagtgttg aatggcaacc
    11101 caactgtcca aaatatcaat gataaagtga cagaggcaaa ccaagctaag gatcaattaa
    11161 atacagcacg tcaaggttta acattagata gacagccagc gttaacaaca ttacatggtg
    11221 catctaactt aaaccaagca caacaaaata atttcacgca acaaattaat gctgctcaaa
    11281 atcatgctgc gcttgaaaca attaagtcta acattacggc tttaaatact gcgatgacga
    11341 aattaaaaga cagtgttgcg gataataata caattaaatc agatcaaaat tacactgacg
    11401 caacaccagc taataaacaa gcgtatgata atgcagttaa tgcggctaaa ggtgtcattg
    11461 gagaaacgac taatccaacg atggatgtta acacagtgaa ccaaaaagca gcatctgtta
    11521 aatcgacgaa agatgcttta gatggtcaac aaaacttaca acgtgcgaaa acagaagcaa
    11581 caaatgcgat tacgcatgca agtgatttaa accaagcaca aaagaatgca ttaacacaac
    11641 aagtgaatag tgcacaaaac gtgcaagcag taaatgatat taaacaaacg actcaaagct
    11701 taaatactgc tatgacaggt ttaaaacgtg gcgttgctaa tcataaccaa gtcgtacaaa
    11761 gtgataatta tgtcaacgca gatactaata agaaaaatga ttacaacaat gcatacaacc
    11821 atgcgaatga cattattaat ggtaatgcac aacatccagt tataacacca agtgatgtta
    11881 acaatgcttt atcaaatgtc acaagtaaag aacatgcatt gaatggtgaa gctaagttaa
    11941 atgctgcgaa acaagaagcg aatactgcat taggtcattt aaacaattta aataatgcac
    12001 aacgtcaaaa cttacaatcg caaattaatg gtgcgcatca aattgatgca gttaatacaa
    12061 ttaagcaaaa tgcaacaaac ttgaatagtg caatgggtaa cttaagacaa gctgttgcag
    12121 ataaagatca agtgaaacgt acagaagatt atgcggatgc agatacagct aaacaaaatg
    12181 catataacag tgcagtttca agtgccgaaa caatcattaa tcaaacaaca aatccaacga
    12241 tgtctgttga tgatgttaat cgtgcaactt cagctgttac ttctaataaa aatgcattaa
    12301 atggttatga aaaattagca caatctaaaa cagatgctgc aagagcaatt gatgcattac
    12361 cacatttaaa taatgcacaa aaagcagatg ttaaatctaa aattaatgct gcatcaaata
    12421 ttgctggcgt aaatactgtt aaacaacaag gtacagattt aaatacagcg atgggtaact
    12481 tgcaaggtgc aatcaatgat gaacaaacga cgcttaatag tcaaaactat caagatgcga
    12541 cacctagtaa gaaaacagca tacacaaatg cggtacaagc tgcgaaagat attttaaata
    12601 aatcaaatgg tcaaaataaa acgaaagatc aagttactga agcgatgaat caagtgaatt
    12661 ctgctaaaaa taacttagat ggtacgcgtt tattagatca agcgaagcaa acagcaaaac
    12721 agcagttaaa taatatgacg catttaacaa ctgcacaaaa aacgaattta acaaaccaaa
    12781 ttaatagtgg tactactgtc gctggtgttc aaacggttca atcaaatgcc aatacattag
    12841 atcaagccat gaatacgtta agacaaagta ttgccaacaa agatgcgact aaagcaagtg
    12901 aagattacgt agatgctaat aatgataagc aaacagcata taacaacgca gtagctgctg
    12961 ctgaaacgat tattaatgct aatagtaatc cagaaatgaa tccaagtacg attacacaaa
    13021 aagcagagca agtgaatagt tctaaaacgg cacttaacgg tgatgaaaac ttagctgctg
    13081 caaaacaaaa tgcgaaaacg tacttaaaca cattgacaag tattacagat gctcaaaaga
    13141 acaatttgat tagtcaaatt actagtgcga caagagtgag tggtgttgat actgtaaaac
    13201 aaaatgcgca acatctagac caagctatgg ctagcttaca gaatggtatt aacaacgaat
    13261 ctcaagtgaa atcatctgag aaatatcgtg atgctgatac aaataaacaa caagagtatg
    13321 ataatgctat tactgcagcg aaagcgattt taaataaatc gacaggtcca aacactgcgc
    13381 aaaatgcagt tgaagcagca ttacaacgtg ttaataatgc gaaagatgca ttgaatggtg
    13441 atgcaaaatt aattgcagct caaaacgcag cgaaacaaca tttaggtact ttaacgcata
    13501 tcactacagc tcaacgtaat gatttaacaa atcaaatttc acaagctaca aacttagctg
    13561 gtgttgaatc tgttaaacaa aatgcgaata gtttagatgg tgctatgggt aacttacaaa
    13621 cggctatcaa cgataagtca ggaacattag cgagccaaaa cttcttggat gctgatgagc
    13681 aaaaacgtaa tgcatacaat caagctgtat cagcagccga aaccatttta aataaacaaa
    13741 ctggaccgaa tacagcgaaa acagcagtcg aacaagcact taataatgtt aataatgcga
    13801 aacatgcatt aaatggtacg caaaacttaa acaatgcgaa acaagcagcg attacagcaa
    13861 tcaatggcgc atctgattta aatcaaaaac aaaaagatgc attaaaagca caagctaatg
    13921 gtgctcaacg cgtatctaat gcacaagatg tacagcacaa tgcgactgaa ctgaacacgg
    13981 caatgggcac attaaaacat gccatcgcag ataagacgaa tacgttagca agcagtaaat
    14041 atgttaatgc cgatagcact aaacaaaatg cttacacaac taaagttacc aatgctgaac
    14101 atattattag cggtacgcca acggttgtta cgacaccttc agaagtaaca gctgcagcta
    14161 atcaagtaaa cagcgcgaaa caagaattaa atggtgacga aagattacgt gaagcaaaac
    14221 aaaacgccaa tactgctatt gatgcattaa cacaattaaa tacacctcaa aaagctaaat
    14281 taaaagaaca agtgggacaa gccaatagat tagaagacgt acaaactgtt caaacaaatg
    14341 gacaagcatt gaacaatgca atgaaaggct taagagatag tattgctaac gaaacaacag
    14401 tcaaaacaag tcaaaactat acagacgcaa gtccgaataa ccaatcaaca tataatagcg
    14461 ctgtgtcaaa tgcgaaaggt atcattaatc aaactaacaa tccgactatg gatactagtg
    14521 cgattaccca agctacaaca caagtgaata atgctaaaaa tggtttaaac ggtgctgaaa
    14581 acttaagaaa tgcacaaaac actgctaagc aaaacttaaa tacattatca cacttaacaa
    14641 ataaccaaaa atctgccatc tcatcacaaa ttgatcgtgc aggtcatgtg agtgaggtaa
    14701 ctgctactaa aaatgcagca actgagttga atacgcaaat gggtaacttg gaacaagcta
    14761 tccatgatca aaacacagtt aaacaaagtg ttaaatttac tgatgcagat aaagctaaac
    14821 gtgatgcgta tacaaatgcg gtaagcagag ctgaagcaat tctgaataaa acgcaaggtg
    14881 caaatacgtc taaacaagat gttgaagcgg ctattcaaaa tgtttcaagt gctaaaaatg
    14941 cattgaatgg tgatcaaaac gttacaaatg cgaagaatgc agctaaaaat gcattaaata
    15001 acttaacgtc aattaataat gcacaaaaac gtgacttaac aactaaaatt gatcaagcaa
    15061 caactgtagc tggtgttgaa gctgtatcta atacgagtac acaattgaat acagcgatgg
    15121 ctaacttgca aaatggtatt aatgataaaa caaatacact agcaagtgaa aactatcatg
    15181 atgctgattc agataagaaa actgcttata ctcaagccgt tacgaacgca gaaaatattt
    15241 taaataaaaa tagtggatca aatttagaca aaactgccgt tgaaaacgcg ttgtcacaag
    15301 ttgctaatgc gaaaggtgcc ctaaatggta accataattt agagcaagct aaatcaaatg
    15361 caaacactac tataaacgga cttcaacatt taacaactgc tcaaaaagat aaattgaaac
    15421 aacaagtgca acaagcacaa aatgttgcag gtgtagatac tgttaaatca agtgccaaca
    15481 cattaaatgg tgctatgggt acgttaagaa atagcataca agataacaca gctacgaaaa
    15541 atggccaaaa ctatcttgat gctacagaac gtaacaaaac aaactataac aatgctgttg
    15601 atagtgctaa tggtgtcatt aatgcaacaa gcaatccaaa tatggatgct aatgcaatta
    15661 accaaatcgc tacacaagtg acatcaacga aaaatgcatt agatggtaca cataatttaa
    15721 cgcaagcgaa acaaacagca acaaatgcca tcgatggtgc tactaactta aataaagcgc
    15781 aaaaagatgc gttaaaagca caagttacaa gtgcgcaacg tgttgcaaat gtaacaagta
    15841 tccaacaaac tgcaaatgaa cttaatacag ctatgggtca attacaacat ggtattgatg
    15901 atgaaaatgc aacaaaacaa actcaaaaat atcgtgacgc tgaacaaagt aagaaaactg
    15961 cttatgatca agctgtagct gctgcgaaag caattttaaa taaacaaaca ggttcaaatt
    16021 cagataaagc agcagttgac cgtgcattac aacaagtaac aagtacgaaa gatgcattga
    16081 atggtgatgc aaaactggca gaagcgaaag cggcagctaa acaaaactta ggcactttaa
    16141 accatattac gaatgcacaa cgtactgact tagaaggcca aatcaatcaa gcgacgactg
    16201 ttgatggcgt taatactgta aaaacaaatg ccaatacatt agacggcgca atgaatagct
    16261 tacaaggttc aatcaatgat aaagatgcga cattaagaaa tcaaaattat cttgatgcgg
    16321 atgaatcaaa acgaaatgca tatacgcaag ctgtcacagc ggctgaaggc attttaaata
    16381 aacaaactgg tggtaacaca tctaaagcag acgttgataa tgcattaaat gcagttacaa
    16441 gagcgaaagc ggctttaaat ggtgctgaca acttaagaaa tgcgaaaact tcagcaacaa
    16501 atacgattga tggtttacct aacttaacac aattacaaaa agacaacttg aagcatcaag
    16561 ttgaacaagc gcaaaatgta gcaggtgtaa atggtgttaa agataaaggt aatacgttaa
    16621 atactgccat gggtgcatta cgtacaagta tccaaaatga taatacgacg aaaacaagtc
    16681 aaaattatct tgatgcatct gacagcaaca aaaataatta caatactgct gtaaataatg
    16741 caaatggtgt tattaatgca acgaacaatc caaatatgga tgctaatgcg attaatggca
    16801 tggcaaatca agtcaataca acaaaagcag cgttaaatgg tgcacaaaac ttagctcaag
    16861 ctaaaacaaa tgcgacgaac acaattaaca acgcacatga cttaaaccaa aaacaaaaag
    16921 atgcattaaa aacacaagtt aacaatgcac aacgtgtatc tgatgcaaat aacgttcaac
    16981 acactgcaac tgaattgaac agtgcgatga cagcacttaa agcagctatt gctgataaag
    17041 aaagaacaaa agcaagcggt aattatgtca atgctgatca agaaaaacgt caagcgtatg
    17101 attcaaaagt gactaacgct gaaaatatca ttagtggtac accgaatgcg acattaacag
    17161 tcaatgacgt aaatagtgcg gcatcacaag tcaatgcggc taaaacagca ttaaatggtg
    17221 ataacaactt acgtgtagcg aaagagcatg ccaacaatac aattgacggc ttagcacaat
    17281 tgaataatgc acaaaaagca aaattaaaag aacaagttca aagtgcaact acattagatg
    17341 gtgttcaaac tgttaaaaat agttctcaaa cgttgaatac agcgatgaaa ggcttaagag
    17401 atagtattgc gaatgaagca acaattaaag caggtcaaaa ctacactgac gcaagtccaa
    17461 ataatcgtaa cgagtacgac agtgcagtta ctgcagcaaa agcaatcatt aatcaaacat
    17521 cgaacccaac gatggaacca aatactatta cgcaagtaac atcacaagtg acaactaaag
    17581 aacaggcatt aaatggtgcg cgaaacttag ctcaagctaa gacaactgcg aaaaacaact
    17641 tgaataactt aacatcaatt aacaatgcac aaaaagatgc gttaacgcgt agcattgatg
    17701 gtgcaacaac agtagctggt gtaaatcaag aaactgcaaa agcaacagaa ttaaataacg
    17761 caatgcatag tttacaaaat ggtatcaatg atgagacaca aacaaaacaa actcagaaat
    17821 acctagatgc agagccaagt aagaaatcag cttatgatca agcagtaaat gcagcgaaag
    17881 caattttaac aaaagctagt ggtcaaaatg tagacaaagc agcagttgaa caagcattgc
    17941 aaaatgtgaa cagtacgaag acggcgttga acggtgatgc gaaattaaat gaagctaaag
    18001 cagctgcgaa acaaacgtta ggtacattaa cacacattaa taatgcacaa cgtacagcgt
    18061 tagacaatga aattacacaa gcaacaaatg ttgaaggtgt taatacagtt aaagccaaag
    18121 cgcaacaatt agatggtgct atgggtcaat tagaaacatc aattcgtgat aaagacacga
    18181 cgttacaaag tcaaaattat caagatgctg atgatgctaa acgaactgct tattctcaag
    18241 cagtaaatgc agcagcaact attttaaata aaacagctgg cggtaataca cctaaagcag
    18301 atgttgaaag agcaatgcaa gctgttacac aagcaaatac tgcattaaac ggtattcaaa
    18361 acttagatcg tgcgaaacag gctgctaaca cagcgattac aaatgcttcg gacttaaata
    18421 caaaacaaaa agaagcatta aaagcacaag taacaagtgc aggacgtgta tctgcagcaa
    18481 atggtgttga acatactgcg actgaattaa atactgcgat gacagcttta aagcgtgcca
    18541 ttgctgataa agctgagaca aaagctagtg gtaactatgt caatgctgat gcgaataaac
    18601 gtcaagcata tgatgaaaaa gttacagctg ccgaaaatat cgttagtggt acaccaacac
    18661 caacgttaac accagcagat gttacaaatg cagcaacgca agtaacgaat gctaagacgc
    18721 agttaaacgg taatcataat ttagaagtag cgaaacaaaa tgctaacact gcaattgatg
    18781 gtttaacttc tttaaatggt ccgcaaaaag caaaacttaa agaacaagtg ggtcaagcga
    18841 cgacgttgcc aaatgttcaa actgttcgtg ataatgcaca aacattaaac actgcaatga
    18901 aaggtctacg agatagcatt gcgaatgaag caacgattaa agcaggtcaa aactacacag
    18961 atgcaagtca aaacaaacaa actgactaca acagtgcagt cactgcagca aaagcaatca
    19021 ttggtcaaac aactagtcca tcaatgaatg cgcaagaaat taatcaagcg aaagaccaag
    19081 tgacagctaa acaacaagcg ttaaacggtc aagaaaactt aagaactgcg caaacaaatg
    19141 cgaagcaaca tttgaacggc ttaagtgact taactgacgc tcaaaaagat gcagtgaaac
    19201 gtcaaatcga aggtgcaacg catgttaatg aagtaacaca agcacaaaat aatgcggatg
    19261 cattaaatac agctatgacg aacttgaaaa atggtattca agatcagaat acgattaagc
    19321 aaggtgttaa cttcactgat gccgacgaag cgaaacgtaa tgcatataca aatgcagtga
    19381 cgcaagctga acaaatttta aataaagcac aaggtccaaa tacttcaaaa gacggtgtcg
    19441 aaactgcgtt agaaaatgta caacgtgcta aaaacgaatt gaacggtaat caaaatgttg
    19501 cgaacgctaa gacaactgcg aaaaatgcat tgaataacct aacatcaatt aataatgcac
    19561 aaaaagaagc attgaaatca caaattgaag gtgcgacaac agttgcaggt gtaaatcaag
    19621 tgtctacaac ggcatctgaa ttaaatacag caatgagcaa cttacaaaat ggtattaatg
    19681 atgaagcagc tacaaaagca gctcagaaat atactgatgc agatagagaa aaacaaactg
    19741 catacaatga cgctgtaaca gcagctaaaa cgttattaga taaaacagct ggttcaaatg
    19801 acaataaagc agctgttgaa caagcattac aacgtgtgaa tactgctaaa acagcattaa
    19861 atggtgacga gcgattaaat gaagcgaaga acacagctaa acaacaagta gcgacaatgt
    19921 cacacttaac tgatgctcaa aaagcaaact taacatcgca aatcgaaagt ggtacgactg
    19981 ttgcaggtgt tcaaggtatt caagctaatg ccggtacttt agatcaagca atgaatcaat
    20041 taagacaaag tattgcttct aaagatgcga ctaaatcaag cgaagattat caagacgcga
    20101 atgcagattt acaaaatgca tacaatgatg cggtaactaa tgctgaaggt attattagtg
    20161 caacgaataa ccctgaaatg aatcctgata caattaacca aaaagcgagc caagtgaaca
    20221 gtgcgaagtc tgcattgaac ggtgatgaaa aattagcagc agcaaaacaa actgcgaaat
    20281 cagatatcgg tcgtttgaca gacttgaaca atgcacaacg aactgcggca aatgctgaag
    20341 tggatcaagc accaaatctt gcagctgtca cagcggctaa aaataaagca acatcgttaa
    20401 acacagcgat gggtaatttg aaacatgcac ttgctgaaaa ggataatacg aaacgtagtg
    20461 tcaattacac agatgcggat caaccaaaac aacaagcgta tgatactgcg gttacacaag
    20521 cagaagcaat tactaatgct aatggcagca acgcgaatga aacacaagtt caagcagcac
    20581 tataccaatt gaatcaagct aaaaatgact tgaatggtga taataaagtt gctcaagcaa
    20641 aagagtcagc gaaacgtgca ttagcttcat atagtaactt gaataatgcg caatcaactg
    20701 cagcaattag tcaaattgac aatgcaacga cagtagcagg cgtaactgct gcacaaaata
    20761 ctgctaatga attaaataca gcaatgggtc aacttcaaaa tggtataaat gaccaaaaca
    20821 ctgttaaaca acaagtgaac tttacagatg ctgaccaagg taagaaagat gcttacacaa
    20881 atgctgttac gaatgctcaa ggtattttag ataaagcaca cggtcaaaat atgacgaaag
    20941 cacaagttga agctgcatta aatcaagtaa cgactgctaa gaatgcttta aacggtgacg
    21001 caaatgtaag acaagcaaaa tcagatgcga aagcaaactt aggtacatta acacacttaa
    21061 ataatgcaca aaaacaagat ttaacatcac aaatcgaagg cgcaacaaca gtcaacggtg
    21121 taaatggtgt taaaacgaaa gcacaagact tagatggtgc aatgcaacga ttacaatcag
    21181 ctatagcaaa taaagatcaa actaaagcga gcgaaaatta catcgacgca gatccaacta
    21241 agaaaacagc atttgataat gctatcacac aagctgaatc ttacttaaat aaagatcacg
    21301 gtgcgaataa agataagcaa gctgttgaac aagcaattca aagtgtaacg tctactgaaa
    21361 atgctttgaa cggtgacgcg aacttacaac gcgctaaaac tgaagctata caagctatcg
    21421 ataacttgac acatttgaat acaccacaaa aaacagcatt aaaacaacaa gtgaacgctg
    21481 cgcaacgtgt atcaggtgta actgatctga aaaatagtgc tacatcactt aataatgcga
    21541 tggatcaatt aaaacaagca attgctgatc atgacacaat tgtagctagt ggtaattaca
    21601 ctaacgcgag tcctgataag caaggtgctt atactgatgc atataatgct gcgaaaaaca
    21661 ttgtaaatgg ttcgcctaat gtgattacaa atgcagcaga tgttacagca gcaacacaac
    21721 gtgttaataa tgctgaaaca ggtttaaacg gtgatacaaa cttagcaact gcgaagcaac
    21781 aagctaaaga tgcattacgt caaatgacac atttatctga tgcacaaaaa caaagtatta
    21841 ctggtcaaat tgatagcgcg acacaagtaa ctggcgttca aagtgtgaaa gacaacgcga
    21901 caaatcttga taatgcaatg aatcaacttc gaaatagtat tgcgaataaa gatgatgtaa
    21961 aagcgagtca accatatgtt gatgcagata gagataaaca aaatgcatac aatacagcag
    22021 ttacaaatgc tgaaaatatc attaatgcaa cgagtcagcc gacacttgat ccatctgcag
    22081 taacacaagc agctaatcaa gtgagcacta acaaaactgc gcttaatggt gcacaaaact
    22141 tagcgaataa aaagcaagaa acgactgcta acatcaacca attaagtcat ttaaataatg
    22201 ctcaaaagca agatttaaat acgcaagtga caaatgcacc aaatattagc acagtaaatc
    22261 aagtgaaaac taaagctgaa caattagatc aagcaatgga acgtttaatc aacggaatcc
    22321 aagacaaaga tcaagtgaaa caaagtgtta actttacaga tgcagatcca gaaaaacaaa
    22381 cagcatacaa caatgcggta actgctgctg aaaatattat taatcaagca aatggtacaa
    22441 atgcgaacca atcacaagtt gaagcagcac tttcaactgt aacaactacg aaacaagcgt
    22501 tgaatggtga tagaaaagta acagatgcta aaaacaatgc aaaccaaaca ttatctacgt
    22561 tagataactt aaacaatgca caaaaaggtg ctgttactgg aaacatcaat caagcgcaca
    22621 ctgtagctga agtaacgcaa gccattcaaa ccgctcagga actgaataca gcgatgggta
    22681 acttgaaaaa tagcttgaat gataaagaca ctacacttgg cagtcaaaac tttgcagatg
    22741 cagatccaga gaagaaaaat gcatacaatg aagcggttca taatgctgaa aatattttaa
    22801 ataaatctac aggtacgaac gtgcctaaag atcaagttga agcagctatg aatcaagtga
    22861 atgctacaaa agcagcgctt aatggtactc aaaaccttga aaaagctaaa caacacgcaa
    22921 atacagcaat tgacggttta agccatttaa caaatgcaca aaaagaggca ttaaaacaat
    22981 tggtacaaca atcgactact gttgcagaag cacaaggtaa tgagcaaaaa gcaaacaatg
    23041 ttgatgcagc aatggacaaa ttacgtcaaa gtattgcaga taatgcgaca acaaaacaaa
    23101 accaaaatta tactgatgca agtcagaata aaaaggatgc gtacaataat gctgtcacaa
    23161 ctgcacaagg tattattgat caaactacaa gtccaacttt agatccgact gttatcaatc
    23221 aagctgctgg acaagtaagc acaactaaaa atgcattaaa tggtaatgaa aacctagagg
    23281 cagcgaaaca acaagcgtca caatcattag gttcattaga taacttaaat aatgcgcaaa
    23341 aacaaacagt tactgatcaa attaatggcg cgcatactgt tgatgaagca aatcaaatta
    23401 agcaaaatgc gcaaaactta aatacagcga tgggtaactt gaaacaagcg atagctgaca
    23461 aagatgctac gaaagcgaca gttaacttca ctgatgcaga tcaagcaaaa caacaagcat
    23521 ataacactgc tgttacaaat gctgaaaata tcatttcaaa agctaatggc ggcaatgcaa
    23581 cacaagctga agttgaacaa gcaatcaaac aagttaatgc tgcaaaacaa gcattaaatg
    23641 gtaatgccaa cgttcaacat gcaaaagacg aagcaacagc attaattaat agctctaatg
    23701 accttaacca agcacaaaaa gacgcattaa aacaacaagt tcaaaatgca actactgtag
    23761 ctggtgtaaa caatgttaaa caaacagcac aagagttaaa caatgctatg acacaattaa
    23821 aacaaggcat tgcagataaa gaacaaacaa aagctgatgg taactttgtc aatgcagatc
    23881 ctgataagca aaatgcatat aatcaagcag tagcgaaagc tgaagcatta attagtgcta
    23941 cgcctgatgt tgtcgttaca cctagcgaaa ttactgcagc gttaaataaa gttacgcaag
    24001 ctaaaaatga tttaaatggt aatacaaact tagcaacggc gaaacaaaat gttcaacatg
    24061 ctattgatca attgccaaac ttaaaccaag cgcaacgtga tgaatacagc aaacaaatca
    24121 cgcaagcaac acttgtacca aacgtcaatg ctattcaaca agcggcgaca acgcttaatg
    24181 acgcgatgac acaattgaaa caaggtattg cgaataaagc acaaattaaa ggtagcgaga
    24241 actatcacga tgctgatact gacaagcaaa cagcatatga taatgcagta acaaaagcag
    24301 aagaattgtt aaaacaaaca acaaatccaa caatggatcc aaatacaatt caacaagcat
    24361 taactaaagt gaatgacaca aatcaagcac ttaacggtaa tcaaaaatta gctgatgcca
    24421 aacaagatgc taagacaaca cttggtacac tagatcattt aaatgatgct caaaaacaag
    24481 cgctaacaac tcaagttgaa caagcaccag atattgcaac agttaataat gttaagcaaa
    24541 atgctcaaaa tctgaataat gctatgacta acttaaacaa tgcattacaa gataaaactg
    24601 agacattaaa tagcattaac tttactgatg cagatcaagc taagaaagat gcttatacta
    24661 atgcggtttc acatgcagaa ggtattttat ctaaagcaaa tggcagcaat gcaagtcaaa
    24721 ctgaagtgga acaagcgatg caacgtgtga acgaagcgaa acaagcattg aatggtaatg
    24781 acaatgtaca acgtgcaaaa gatgcagcga aacaagtgat tacaaatgca aatgatttaa
    24841 atcaagcgca aaaagatgca ttaaaacaac aagtcgatgc tgcgcaaact gttgcaaatg
    24901 taaacacgat taagcaaaca gcacaagatt taaatcaagc aatgacacaa ttgaaacaag
    24961 gtattgcaga taaagaccaa actaaagcaa atggtaactt tgtcaatgct gatactgata
    25021 agcaaaatgc ttacaacaat gcggtagcac atgctgaaca aataattagt ggtacaccaa
    25081 atgcaaacgt ggatccacaa caagtggctc aagcgttaca acaagtgaat caagctaagg
    25141 gtgatttaaa cggtaaccat aacttacaag ttgctaaaga caatgcaaat acagccattg
    25201 atcagttacc aaacttaaat caaccacaaa aaacagcatt aaaagaccaa gtgtcgcatg
    25261 cagaacttgt tacaggtgtt aatgctatta agcaaaatgc tgatgcgtta aataatgcaa
    25321 tgggtacatt gaaacaacaa attcaagcga acagtcaagt accacagtca gttgacttta
    25381 cacaagcgga tcaagacaaa caacaagcat ataacaatgc ggctaaccaa gcgcaacaaa
    25441 tcgcaaatgg cataccaaca cctgtattga cgcctgatac agtaacacaa gcagtgacaa
    25501 ctatgaatca agcgaaagat gcattaaacg gtgatgaaaa attagcacaa gcgaaacaag
    25561 aagctttagc aaatcttgat acgttacgcg atttaaatca accacaacgt gatgcattac
    25621 gtaaccaaat caatcaagca caagcgttag ctacagttga acaaactaaa caaaatgcac
    25681 aaaatgtgaa tacagcaatg agtaacttga aacaaggtat tgcaaacaaa gatactgtca
    25741 aagcaagtga gaactatcat gatgctgatg ccgataagca aacagcatat acaaatgcag
    25801 tgtctcaagc ggaaggtatt atcaatcaaa cgacaaatcc aacgcttaac ccagatgaaa
    25861 taacacgtgc attaactcaa gtgactgatg ctaaaaatgg cttaaacggt gaagctaaat
    25921 tggcaactga aaagcaaaat gctaaagatg ccgtaagtgg gatgacgcat ttaaacgatg
    25981 ctcaaaaaca agcattaaaa ggtcaaatcg atcaatcgcc tgaaattgct acagtgaacc
    26041 aagttaaaca aacagcaacg agcctagatc aagcaatgga tcaattatca caagctatta
    26101 atgataaagc tcaaacatta gcggacggta attacttaaa tgcagatcct gacaaacaaa
    26161 atgcgtataa acaggcagta gcaaaagctg aagcattatt gaataaacaa agtggtacta
    26221 atgaagtaca agcacaagtt gaaagcatca ctaatgaagt gaacgcagcg aaacaagcat
    26281 taaatggtaa tgacaatttg gcaaatgcaa aacaacaagc aaaacaacaa ttggcgaact
    26341 taacacactt aaatgatgca caaaaacaat catttgaaag tcaaattaca caagcgccac
    26401 ttgttacaga tgtcactacg attaatcaaa aagcacaaac gttagatcat gcgatggaat
    26461 tattaagaaa tagtgttgcg gataatcaaa cgacattagc gtctgaagat tatcatgatg
    26521 caactgcgca aagacaaaat gactataacc aagctgtaac agctgctaat aatatcatta
    26581 atcaaactac atcgcctacg atgaatccag atgatgttaa tggtgcaacg acacaagtga
    26641 ataatacgaa agttgcatta gatggtgatg aaaaccttgc agcagctaaa caacaagcaa
    26701 acaacagact tgatcaatta gatcatttga ataatgcgca aaagcaacag ttacaatcac
    26761 aaattacgca atcatctgat attgctgcag ttaatggtca caaacaaaca gcagaatctt
    26821 taaatactgc gatgggtaac ttaattaatg cgattgcaga tcatcaagcc gttgaacaac
    26881 gtggtaactt catcaatgct gatactgata aacaaactgc ttataataca gcggtaaatg
    26941 aagcagcagc aatgattaac aaacaaactg gtcaaaatgc gaaccaaaca gaagtagaac
    27001 aagctattac taaagttcaa acaacacttc aagcgttaaa tggagaccat aatttacaag
    27061 ttgctaaaac aaatgcgacg caagcaattg atgctttaac aagcttaaat gatcctcaaa
    27121 aaacagcatt aaaagaccaa gttacagctg caactttagt aactgcagtt catcaaattg
    27181 aacaaaatgc gaatacgctt aaccaagcaa tgcatggttt aagacagagc attcaagata
    27241 acgcagcaac taaagcaaat agcaaatata tcaacgaaga tcaaccagag caacaaaact
    27301 atgatcaagc tgttcaagcc gcaaataata ttatcaatga acaaactgca acattagata
    27361 ataatgcgat taatcaagca gcgacaactg tgaatacaac gaaagcagca ttacatggtg
    27421 atgtgaagtt acaaaatgat aaagatcatg ctaagcaaac ggttagtcaa ttagcacatc
    27481 taaacaatgc acaaaaacat atggaagata cgttaattga tagtgaaaca actagaacag
    27541 cagttaagca agatttgact gaagcacaag cattagatca acttatggat gcattacaac
    27601 aaagtattgc tgacaaagat gcaacacgtg cgagcagtgc atatgtcaat gcagaaccga
    27661 ataaaaaaca atcctatgat gaagcagttc aaaatgctga gtctatcatt gcaggattaa
    27721 ataatccaac tatcaataaa ggtaatgtat caagtgcgac tcaagcagta atatcatcta
    27781 aaaatgcatt agatggtgtt gaacgattag ctcaagataa gcaaactgct ggaaattctc
    27841 taaatcattt agatcaatta acaccagctc aacaacaagc gctagaaaat caaattaata
    27901 atgcaacaac tcgtggcgaa gtagcacaaa aattaactga agcacaagca cttaaccaag
    27961 caatggaagc tttacgtaat agcattcaag atcaacagca aacggaagcg ggtagcaagt
    28021 ttatcaatga agataaacca caaaaagatg cttaccaagc agcagttcaa aatgcaaaag
    28081 atttaattaa tcaaactaac aatccaacgc ttgataaagc acaagttgaa caattgacac
    28141 aagctgttaa ccaagctaaa gataacctac acggtgatca aaaacttgca gacgataaac
    28201 aacatgcggt tactgattta aatcaattaa atggtttgaa taatccgcaa cgtcaagcac
    28261 ttgaaagcca aataaacaac gcagcaactc gtggcgaagt agcacaaaaa ttagctgaag
    28321 caaaagcgct tgatcaagca atgcaagcat tacgtaatag tattcaagat caacaacaaa
    28381 cagaatctgg tagcaagttt atcaatgaag ataaaccgca aaaagatgct taccaagcag
    28441 cagttcaaaa tgcaaaagat ttaattaacc aaacaggtaa tccaacactc gacaaatcac
    28501 aagtagaaca attgacacaa gcagtaacaa ctgcaaaaga taatctacat ggtgatcaaa
    28561 aacttgctcg tgatcaacaa caagcagtaa caactgtaaa tgcattgcca aacttaaatc
    28621 atgcacaaca acaagcatta actgatgcta taaatgcagc gcctacaaga acagaggttg
    28681 cacaacatgt tcaaactgct actgaacttg atcacgcgat ggaaacattg aaaaataaag
    28741 ttgatcaagt gaatacagat aaggctcaac caaattacac tgaagcgtca actgataaaa
    28801 aagaagcagt agatcaagcg ttacaagctg cagaaagcat tacagatcca actaatggtt
    28861 caaatgcgaa taaagacgct gtagaccaag tattaactaa gcttcaagaa aaagaaaatg
    28921 agttaaatgg taatgagaga gtcgctgaag ctaaaacaca agcgaaacaa actattgacc
    28981 aattaacaca tttaaatgct gatcaaattg caactgctaa acaaaacatt gatcaagcga
    29041 cgaaacttca accaattgct gaattagtag atcaagcaac gcaattgaat caatctatgg
    29101 atcaattaca acaagcagtt aatgaacatg ctaacgttga gcaaactgta gattacacac
    29161 aagcagattc agataaacaa aatgcttata aacaagctat tgctgatgct gaaaatgtat
    29221 tgaaacaaaa tgcgaataag caacaagtgg atcaagcact tcaaaatatt ttaaatgcaa
    29281 aacaagcatt aaatggtgat gaacgtgtag cacttgctaa aacaaatggt aaacatgaca
    29341 tcgaccaatt gaatgcatta aacaatgctc aacaagatgg atttaaaggt cgcatcgatc
    29401 aatcaaacga tttaaatcaa atccaacaaa ttgtagatga ggctaaggca cttaatcgtg
    29461 caatggatca attgtcacaa gaaatcactg acaatgaagg acgcacgaaa ggtagcacga
    29521 actatgtcaa tgcagataca caagtcaaac aagtatatga tgaaacggtt gataaagcga
    29581 aacaagcact tgataaatcg actggtcaaa acttaactgc aaaacaagtt atcaaattaa
    29641 atgatgcagt cactgcagct aagaaagcat taaatggtga agaaagactt aataatcgta
    29701 aagctgaagc attacaaaga ttggatcaat taacacatct aaacaatgct caaagacaat
    29761 tagcaatcca acaaattaat aatgctgaaa cgctaaataa agcatctcga gcaattaata
    29821 gagcaactaa attagataat gcaatgggtg cagtacaaca atatattgac gaacagcacc
    29881 ttggtgttat cagcagcaca aattacatca atgcagatga caatttgaaa gcaaattatg
    29941 ataatgcaat tgcgaatgca gcacatgagt tagataaagt gcaaggtaat gcaattgcaa
    30001 aagctgaagc agagcaattg aaacaaaata ttatcgatgc tcaaaatgca ttaaatggag
    30061 accaaaacct tgcaaatgcc aaagataaag caaatgcgtt tgttaattcg ttaaatggat
    30121 taaatcaaca gcaacaagat cttgcacata aagcaattaa caatgccgat actgtatcag
    30181 atgtaacaga tattgttaat aatcaaattg acttaaatga tgcaatggaa acattgaaac
    30241 atttagttga caatgaaatt ccaaatgcag agcaaactgt caattaccaa aacgctgacg
    30301 ataatgctaa aacaaacttc gatgatgcca aacgtctagc aaatacattg ctaaatagtg
    30361 ataacacaaa tgtgaatgat atcaatggcg caatccaagc agtcaatgat gcaatccata
    30421 atcttaatgg tgatcaacga ctacaagatg ctaaagacaa ggcaattcaa tctattaatc
    30481 aagctttagc taataagcta aaagaaatcg aagcttcaaa tgcgacggat caagacaagc
    30541 ttattgcgaa aaataaagca gaagaattgg caaacagcat catcaacaac attaataaag
    30601 caacaagtaa tcaggctgta tctcaagttc aaacagcagg caaccacgcg attgaacaag
    30661 tgcatgccaa tgaaatacca aaagcaaaaa ttgatgccaa taaagacgtt gataagcaag
    30721 ttcaagcatt aattgacgaa attgatcgaa atccaaatct aacagataag gaaaaacaag
    30781 cacttaaaga tcgtattaat caaatacttc aacaaggtca taacggcatt aacaatgcga
    30841 tgactaaaga agaaattgaa caagccaaag cacaacttgc gcaagcatta caagacatca
    30901 aagatttagt gaaagctaaa gaagatgcga aacaagatgt tgataaacaa gttcaagctt
    30961 taattgacga aatcgatcaa aatccaaatc taacagataa ggaaaaacaa gcacttaaat
    31021 atcgaattaa tcaaatactt caacaaggtc ataacgacat taacaatgcg ctgactaaag
    31081 aagaaattga gcaggcaaaa gcacaacttg cacaagcatt gcaagacatc aaagatttag
    31141 tgaaagctaa agaagatgcg aaaaatgcaa taaaagcctt agctaatgcg aagcgtgatc
    31201 aaatcaattc aaatccagat ttaacacctg agcaaaaagc aaaagcgctc aaagaaattg
    31261 acgaagctga aaaacgagca ctacaaaacg ttgagaatgc tcaaactata gatcaattaa
    31321 atcgaggatt aaacttaggt ttagatgaca ttagaaatac acatgtatgg gaggttgatg
    31381 aacaacctgc tgtaaatgaa atttttgaag caacacctga gcaaatccta gttaatggtg
    31441 aactcattgt acatcgtgat gacatcatta cagaacaaga tattcttgca cacataaact
    31501 taattgatca gctttcagca gaagtcatcg atacaccatc aactgcaacg atttctgata
    31561 gcttaacagc aaaagttgaa gttacattgc ttgatggatc aaaagtgatt gttaatgttc
    31621 ctgtaaaagt tgtagaaaaa gaattgtcag tagtcaaaca acaggcaatt gaatcaatcg
    31681 aaaatgcggc acaacaaaag attaatgaaa tcaataatag tgtgacatta acactggaac
    31741 aaaaagaagc tgcaattgca gaagttaata agcttaaaca acaagcaatt gatcatgtta
    31801 acaatgcacc tgatgttcat tcagttgaag aaattcaaca acaagaacaa gcgcatattg
    31861 aacaatttaa tccagaacaa tttacgattg aacaagcaaa atcaaatgca attaaatcga
    31921 ttgaagatgc aattcaacat atgattgatg aaatcaaagc tcgtactgat ctaacagata
    31981 aagagaagca agaagctatt gctaagttaa atcaattaaa agaacaagca attcaagcga
    32041 ttcaacgtgc gcaaagcatc gatgaaataa gtgagcaatt ggaacaattt aaagctcaaa
    32101 tgaaagcagc taatccaaca gcaaaagaac tagctaaacg caagcaagaa gctattagta
    32161 gaattaaaga cttttcaaat gaaaaaataa atagtattcg aaatagtgaa attggcacag
    32221 ctgatgaaaa acaagcagca atgaatcaaa ttaacgaaat tgtgcttgaa acaattagag
    32281 atattaataa tgcgcataca ttacagcaag ttgaggctgc attgaacaat ggtattgctc
    32341 gaatttcagc agtacaaatt gtaacatctg atcgtgctaa acaatcgtca agtactggaa
    32401 atgaatctaa tagccattta acaattggtt atggaactgc aaatcatcca tttaacagtt
    32461 cgactattgg acataaaaag aaacttgatg aagatgatga cattgatcca cttcatatgc
    32521 gtcactttag taataatttc ggtaatgtta ttaaaaacgc tattggtgtg gtgggtatct
    32581 ctggtttact agctagtttc tggttcttca ttgccaaacg tcgtcgtaaa gaagatgaag
    32641 aggaagaatt agaaataaga gataataata aagattcaat aaaagagact ttagacgata
    32701 caaaacattt accactttta tttgcgaaac gtcgcagaaa agaagatgaa gaagatgtta
    32761 ctgttgaaga aaaagattcg ctaaataatg gcgagtcact cgataaagtt aaacatacgc
    32821 cgttcttctt accaaaacgt cgtcgtaaag aagatgaaga agatgtggaa gttacaaatg
    32881 aaaacacaga tgaaaaagtg ttgaaagata acgaacattc accactctta ttcgcaaaac
    32941 gacgcaaaga taaagaggaa gatgttgaaa caacaactag tattgaatct aaagatgagg
    33001 acgttccttt attattggct aaaaagaaaa atcaaaaaga taaccaatcc aaagacaaaa
    33061 agtcagcatc aaaaaatact tctaaaaagg tagcagctaa aaagaagaaa aagaaagcta
    33121 agaaaaataa aaaataattt gtttctttga taaatagagg agcaccgatt gacatcacat
    33181 cagtcggtgc tccttttatt tattcttttt aattaattta tacaatgcct gttgagcgtg
    33241 ttgattcgct tctttgtttt gttctctcgg tatccattta acaaataata aatcaaaatc
    33301 tttttcaaat atttctattt gatcaaaata aggtttgaaa tttgcgtttt tcacataacc
    33361 agcttcaatg ctatctgcaa ttagctttga gtctgtatat aatagtgcgt tttgaacatt
    33421 taattcacgt gcatgttcta gtgcataaat acatgcagcc cattctgcag tgtggttatc
    33481 catttcgcct aactcatgtg tatatgtata atgctgctca tcttctttga ttacaatggc
    33541 acatgtactt atgcctggat ttcctttcgt cgcagcatca aaatttattt tcgccataat
    33601 aaacctactt tctattcaat acttagttaa agttactatt actgtaatac aaaatatgtt
    33661 gggtaatcca ttaaaaaaca cgcatcactt aaataagtaa cacgtgttta aaatactcgc
    33721 tgattcaaag atgattttct aatacgtagt actgtaatat acttcctaaa aaaatcatct
    33781 tcaggctggg acataaatca atgttctatg ctctacgatg ttatattggc agtagttgac
    33841 tgaacgaaaa tgcgcttgta acaagctttt ttcaattcta gtcaggggcc ccaacacaga
    33901 gaatttcgaa aagaaattct acaggcaatg cgagttgggg tgtgggtccc aacacagaag
    33961 atgacgaaaa gtcagcttac aataatgtgc aagtttggga tgggccccaa caaagagaaa
    34021 ttggattccc aatttctaca gacaatgcaa gttggggtgg gacgacgaaa taaattttgc
    34081 gaaaatatta tttctgtccc actcccttaa aacttattct tttgtgtagt aagtgcgtta
    34141 atagccttga tctaacttat caatcttacc tttacgataa aatgatttag caatatatcc
    34201 aaatggtaca ttgaaaactg ttgaagctaa ttttaatacg tacgttgtaa taaatatttc
    34261 aaatacaact gtaccaggta aacttccgat aaatgcgata gctacaaata aagctgtatc
    34321 aattattgag cttaaaaatg tactaccata tgcacggatg aaaaacgttt tatcagaact
    34381 aaatactttt ttaattagtg aaaagataaa tacatcaata tgttgaccaa taatatatgc
    34441 gacgattgag cctaaagcaa tgcgtggcac aacatcaaag attgcgtgta atgctttttg
    34501 tgccatatct tctggtgcag gaataaaatg taatgacaat tgcataacaa taatcataat
    34561 taatgttgat gaaaagccta accaaactgc tctttttgca actctacgcc cataaatatc
    34621 gtttaatata tctgttgcta aataaataga agcaaacatg acattaccta aagttgctga
    34681 aataccaaag atttctacag ttttaatcac ttgtatgttg gcaatgattg tgccaattgc
    34741 aacccatgca attaaacctt gtttaccaaa aaagcgatac ataagtacca taagcacgaa
    34801 cgttgcaata aacgtaacta gtcctaaaat ttcattatac atattaaaat gtcctcctaa
    34861 attttgatca tgcgggtgtt tagaaaccgc tcaataaata actaactcat tttactgaaa
    34921 aatcgtttgc gatgcaatag catttgaata taaatacatt ttacgtatag aattgtaaaa
    34981 ggtttcattc aaatcttagg gtcaaaaatg ttataatatt tttatgtcaa atttaaaaca
    35041 gtaacactta tttacaaggt tgcaatattt tgaagtaata aaggaagtgt cgcgtatttt
    35101 aactttttca gagcaaaatg cactcgcgaa aatagatgat ttaatgaata cttattgcaa
    35161 tcaatgtcca atcaaaactc gtctgcgtaa attagagggg aaaacgaagg cgcatcattt
    35221 ttgtatcaat gagtgttcaa tagggaaaga aataaaacaa ttaggaaatg aacttcaata
    35281 ggaggaagtc aaatgaaaat tatatctata tcagaaacac cgaaccacaa cacaatgaag
    35341 attacactta gtgaaagcag agaaggtatg acatcagata cgtatactaa agttgatgat
    35401 tcacagccag catttattaa tgacatctta aaggttgaag gcgttaaatc aattttccat
    35461 gttatggact ttatttcagt agataaagaa aatgacgcaa attgggaaac agtattgcca
    35521 aaagtagagg ctgtattcga ataaattttt catcaactag tattcggggg gaataaagta
    35581 tatggaaatt ttacgtatag agccaacacc aagtccaaat acaatgaaag ttgttttgtc
    35641 atatacaaga gaagacaagt tatctaatac ttataaaaaa gtagaagaaa cacaaccaag
    35701 atttataaat cagttgttat ctatagatgg tatcacttcc atttttcatg tcatgaactt
    35761 cttagctgtt gataaggcac caaaagctga ttgggaagtc atattacctg atattaaagc
    35821 tgctttttct gatgcgaata aggttttaga atctgtaaat gaacctcaaa ttgacaatca
    35881 ttttggtgaa attaaagctg aattattaac ttttaagggt ataccgtatc aaattaagct
    35941 aacttctgct gaccaagaat taagagaaca attaccacaa acatatgttg accatatgac
    36001 tcaagcgcaa acagcacatg acaatattgt ttttatgcgt aaatggctag atttaggaaa
    36061 tcgctatgga aatattcaag aagtaatgga tggtgtccta gaagaagtgc taactaccta
    36121 tccagaatca cagttacccg tattggtaaa acatgcttta gaagaaaatc acgcaactaa
    36181 taattatcat ttctatcgac atgtctcttt ggatgaatat catgcaactg ataattggaa
    36241 gactcgatta cgaatgttaa accattttcc aaagccgact tttgaagata taccgctgct
    36301 tgatttagct ttatctgatg aaaaagtacc ggttagacgt caagcgattg tattattagg
    36361 tatgattgaa agtaaagaaa ttttaccgta tttatataag gggcttcgtg ataaaagtcc
    36421 tgttgtaaga agaacagcag gggattgcat aagcgattta gggtatccag aggcactacc
    36481 agaaatggtg ctactattag atgatccaca gaaaatcgtt aggtggcgtg ctgctatgtt
    36541 tatctttgat gaaggtaatg cagagcagct tcccgcacta aaagcccata ttaatgacaa
    36601 tgcgtttgaa gttaaattac aaattgaaat ggccatatct cgtattgaaa acggagatga
    36661 agcacttggt tcagtatgga aacaaatggc aaacagaaca atttaattgg aggaattaaa
    36721 tatgaatgca tatgatgctt atatgaaaga aattgcgcaa caaatgcgtg gcgaattaac
    36781 tcaaaatggt tttacaagtt tagaaacgag cgaagcagta tcggagtata tgaaccaagt
    36841 aaatgctgat gacactactt ttgtagttat taactctaca tgcggctgtg cagctggatt
    36901 agcaagacca gctgcagtag cagttgcaac acaaaatgaa catagaccta caaatacagt
    36961 tacagttttt gctgggcaag ataaagaagc aactgctaca atgcgagaat tcattcagca
    37021 agcaccatct agtccttcgt atgctttatt caaaggtcaa gatttagttt attttatgcc
    37081 tagagaattt atcgaaggta gagatattaa tgacattgca atggacttaa aggatgcctt
    37141 tgacgaaaat tgtaaatagt acacataaat aaatataaag gttaacacat tttataatat
    37201 taaaaatggt gtctgtcatt gaaaatagag aatatagttg tattctattt gttaaataaa
    37261 gtccgttttt accaactata ttttctagaa atttaactgt tttaatagga catcaaacat
    37321 aatattcaaa tcatgtgtta acctcttttt taaaattttt tagcattaaa gttatagatt
    37381 tgggtaaaca attaccaatt ggaaacatat atcacgttac gatggggtag gtacttaatc
    37441 agcattttat aaataaagta acggaattca tgatattaat atcatattcc taaaatgagt
    37501 gataacaaaa tgctacataa agttaagtta tatcaaacta aatatacata ctataaataa
    37561 tgaaaatgag gtgttatcgc atatgttgaa ttcatttgat gcagcatatc acagtctttg
    37621 tgaagaagtt ttagaaatag gaaatacacg aaatgatcgc acaaatacag gtacgatttc
    37681 gaaatttggt catcaacttc gctttgactt atctaaagga tttccactat taacgacaaa
    37741 gaaagtttct tttaaattag tagcaaccga attattatgg ttcattaaag gagatacaaa
    37801 catccaatac ttattaaaat ataataataa tatatggaac gaatgggctt ttgaaaatta
    37861 tatcaaatca gacgagtata aaggtccaga tatgacagat ttcgggcatc gtgcattgag
    37921 tgatcctgaa tttaacgaac aatataaaga acaaatgaaa caatttaagc aacgtattct
    37981 tgaagatgat acatttgcga agcaattcgg ggatttagga aatgtttatg gtaaacaatg
    38041 gcgagattgg gttgataaag atggtaatca ttttgatcaa cttaaaacag taattgaaca
    38101 aattaagcat aatccagatt caaggcgaca catcgtatct gcatggaatc caacagaaat
    38161 tgatacaatg gcacttccgc cttgtcatac catgttccag ttttatgtcc aagatggtaa
    38221 gttaagttgc cagttatacc aacgtagcgc agatatcttt ttaggtgtgc catttaatat
    38281 cgcaagctac gctttattga cacaccttat tgccaaagaa tgtggacttg aagtgggtga
    38341 atttgtgcat acatttggag atgcacatat ttattcaaat catattgatg cgattcaaac
    38401 acaattagca cgtgaaagct tcaatcctcc aacattaaaa attaacagtg acaagtctat
    38461 tttcgacata aattatgaag atttggaaat tgttgactat gaatcacatc cagcaataaa
    38521 agctccaata gcagtgtagt cattgcatag ttagctaacc atatagacat caaaatgaca
    38581 tcatagtatt ttcaagtgca aaaaagtact tttttgtgtt aaacgttttc ataaattatg
    38641 caaaatcatt atttctatca cactttatga taaaaattgt gttaaattaa agataactta
    38701 gtaataaaaa atgaaatgat agaagaagga ggataattat gactttatcc attctagttg
    38761 cacatgactt gcaacgagta attggttttg aaaatcaatt accttggcac ctaccaaatg
    38821 atttgaagca tgttaaaaaa ttatcaacag gtcatacttt agtaatgggt cgtaagacat
    38881 ttgaatcgat tggtaaacca ctaccgaatc gtcgaaatgt tgtacttact tcagatacaa
    38941 gtttcaacgt agagggcgtt gatgtaattc actctattga agatatttac caactaccgg
    39001 gccatgtttt catatttgga gggcaaacat tatttgaaga aatgattgat aaagtggacg
    39061 acatgtatat tactgttatt gaaggtaaat tccgtggtga tacgttcttt ccaccttata
    39121 catttgaaga ctgggaagtt gcctcttcag ttgaaggtaa actagatgag aaaaatacaa
    39181 ttccacatac ctttctacat ttaattcgta aaaaataagg gggaaaacga ccatgacaaa
    39241 acagattata gtaacagact caacatccga tttatctaaa gaatacttag aagcaaacaa
    39301 cattcatgta attcctttaa gtttaactat tgaaggagct tcatacgttg accaagtaga
    39361 tattacatca gaagaattta ttaatcatat tgaaaatgat gaagatgtaa agacaagtca
    39421 gccagccata ggtgaattta tatctgctta tgaagaacta ggaaaagatg gctctgaaat
    39481 cataagtatt catctttctt caggattaag tggtacatat aacactgctt accaagcaag
    39541 tcaaatggta gatgctaatg taactgttat tgattcaaaa tctatttctt ttggtttagg
    39601 gtatcaaata caacacctag tagagcttgt aaaagaaggt gtctcaactt ctgaaatagt
    39661 taaaaagtta aatcatttaa gagaaaacat taaattattt gtagttatag ggcaattgaa
    39721 tcaattaatt aaaggtggca gaattagtaa aacaaaaggt ttgattggta atcttatgaa
    39781 aattaaacca attggtacac tagatgatgg tcgcttagag cttgtgcaca atgcgagaac
    39841 tcaaaattct agtatccaat acttgaaaaa ggaaattgct gaatttatag gagatcatga
    39901 aatcaaatcc attggtgtcg cacatgctaa cgtcattgaa tatgttgata aattgaagaa
    39961 agtttttaat gaagcttttc atgtgaataa ttacgatata aatgtaacta caccagttat
    40021 ttctgcacat actggtcaag gtgcgattgg cctcgtagtc cttaagaagt aaatttaatc
    40081 ttttcagtgt taattacttc catttcaatc ctttatagac taaatttata attagataga
    40141 tagaggaggt aattcatatg acaaaagaat atgcaacatt agcaggagga tgtttctggt
    40201 gcatggttaa accatttaca tcatatccag gcatcaagtc agtcgtatct ggttatagtg
    40261 gcggtcatgt tgacaaccca acttatgaac aggtatgtac gaatcaaacc ggccatgtcg
    40321 aagcagtaca aattacgttt gatccagagg ttacttcctt tgaaaatata ttagacatat
    40381 atttcaaaac atttgaccca actgatgatc aagggcaatt tttcgataga ggcgaaagct
    40441 atcaaccagt cattttctat catgatgaac atcagaaaaa ggctgctgag tttaaaaagc
    40501 aacaattaaa tgaacaaggt attttcaaga aaccagtgat tacacctatt aaaccatata
    40561 aaaatttcta tccagctgaa gactaccatc aagattatta caaaaagaac ccggtacatt
    40621 attaccaata tcaacgtggt tcaggtagaa aagcgtttat agaatcacat tgggggaatc
    40681 aaaatgctta aaaaagataa aagtgaacta acagatatag aatatattgt tacacaagaa
    40741 aacggcactg aaccaccatt tatgaatgaa tattggaatc attttgctaa aggaatttat
    40801 gtagataaaa tttctggtaa acctttattt acatctgaag aaaagtttca ttctgaatgt
    40861 ggatggccta gcttttccaa agcgcttgat gacgatgaaa ttatagaatt agtcgacaaa
    40921 tcatttggta tgttgagaac tgaagtgcgt tcagaagaat caaatagtca tttaggacat
    40981 gtctttaatg atggacctaa agaaagtggc ggcttaagat actgtatcaa ttccgctgca
    41041 attcaattta ttccatatga aaagttagaa gaattgggtt atggcgattt aatatcacat
    41101 tttgataagt aggagtggaa agtatgttta aaaaattatt cggaaaaggc aaagaagttc
    41161 agaaagatat cgcaatttat gcaccattaa ctggagaatt cgtgaaaatt gaagatattc
    41221 cagatcctgt attcgcacaa aaaatgatgg gcgaaggttt tggtattaat ccaactgaag
    41281 gagaagttgt gtctccaatt gcaggacgtg ttgacaatgt ctttccaact aagcatgcga
    41341 ttgggttaaa agcagataac ggattagaat tattagttca tatcggttta gacacagttc
    41401 aattagacgg tgaaggattt gaagtgttag tgtctagtgg tgacgaagtt aacgtcggag
    41461 atccattagt aagattcaac cttgaatata ttaataataa cgctaaatct gtaatttcac
    41521 caattataat tactaacact gatcaagcag cttcaattaa tatttatgat gaaaatgctg
    41581 tgattaaagg tgaaacaaaa gtgattgatg tgacaatgaa ctaatgaaaa actattcgtt
    41641 ttatcaattt gtcatgacag ttcgtggtcg acacgacgat aaaggtcgtc tagcagaaga
    41701 gatatttgac gatcttgctt tcccaaaaca cgatgatgat tttaacatac tgtctgatta
    41761 tattgagaca catggtgatt tcacattgcc aatgtctgta tttgatgatt tatatgaaga
    41821 atatacggaa tggctaaaat tttaatataa tttttaataa taatagttag aaccagggtg
    41881 atgcaattcg ttatcctggt tttaatttaa aataaactaa gttgtgacta aaaattaatc
    41941 aattatagtg aaatatggtg cgctatcttg cataaattga tatgattaac tacacagaat
    42001 ttaaaagtac ataatacata ataaggaagt gatacaatgg atgataagca acacacatct
    42061 tcatccgatg atgaacgcgc tgaaattgca acaagcaatc aagaccaaga aactaattca
    42121 tcgaaacgcg ttcacttaaa acgttggcaa ttcatatcaa tattaattgg tacaatccta
    42181 ataacagctg tcatcacagt tgttgcatat atttttataa atcaaaaaat aagtggttta
    42241 aacaaaactg atcaagcaaa cttaaataaa attgaaaatg tgtataaaat cttaaatagt
    42301 gattattaca aaaaacagga ctctgacaag ttaagtaaag ctgcaattga tggcatggtc
    42361 aaagaattaa aagatcctta ttctgaatat ttaacaaaag aacaaacgaa atcctttaat
    42421 gaaggtgttt caggtgattt tgtaggtatt ggtgcagaaa tgcaaaagaa aaatgatcaa
    42481 attatggtta ctagtcctat gaagggatct ccagcagaac gtgctggcat tcgtcctaaa
    42541 gatgtcatta ctaaagtaaa tggaaaatca attaaaggta aagcattaga tgaagttgtc
    42601 aaagatgttc gtggtaaaga aaacactgaa gtcactttaa ctgttcaacg aggtagtgaa
    42661 gaaaaagacg ttaagattaa acgtgaaaaa attcatgtta aaagtgttga gtataagaaa
    42721 aaaggtaaag ttggagttat tactattaat aaattccaga atgatacatc aggtgaattg
    42781 aaagatgcag ttctaaaagc tcacaaagat ggtttgaaaa agattgtttt agatttaaga
    42841 aataatccag gtggactact agatgaagct gttaaaatgg caaatatttt tatcgataaa
    42901 ggaaaaactg ttgttaaact agaaaaaggt aaagatactg aagcaattca aacttctaat
    42961 gatgcgttaa aagaagcgaa agacatggat atatccatct tagtgaatga aggttcagct
    43021 agcgcttctg aagtgtttac tggtgcgcta aaagactata ataaagctaa agtttatggg
    43081 tcaaaaacat tcggcaaagg tgtcgtacaa actacaagag agtttaagga tggttcattg
    43141 ttaaaatata ctgaaatgaa atggttaacg ccagatggtc attatattca cggtaaaggc
    43201 attaaaccag acgttactat tgacacacct aaatatcaat ctttaaatgt cattcctaat
    43261 acgaaaacat ttaaagttgg agacgatgat aaaaatatta aaactattaa aattggttta
    43321 tcagctttag gttataaagt tgataatgaa tcaacgcaat ttgataaagc tttagaaaat
    43381 caagttaaag ctttccaaca agcgaataaa cttgaggtaa ctggtgaatt taataaagaa
    43441 acgaataata aatttactga gttattagtt gaaaaagcta ataaacatga tgatgttctc
    43501 gataagttga ttaatatttt aaaataagcg atacacacta ctaaaattgt attattatta
    43561 tgttaatgac acgcctcc
//
LOCUS       DANJFZ010000006        43477 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.006, whole genome shotgun sequence.
ACCESSION   DANJFZ010000006 DANJFZ010000000
VERSION     DANJFZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.006"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            15..1466
                     /locus_tag="PWN45_000268"
     CDS             15..1466
                     /locus_tag="PWN45_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0622067.1"
                     /translation="MFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIG
                     AAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENS
                     MKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTP
                     VGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLI
                     TDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIAD
                     HIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDG
                     RPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYF
                     FNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIA
                     YNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(1780..2922)
                     /locus_tag="PWN45_000269"
     CDS             complement(1780..2922)
                     /locus_tag="PWN45_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDL0622068.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            3363..4940
                     /locus_tag="PWN45_000270"
     CDS             3363..4940
                     /locus_tag="PWN45_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDL0622069.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            4966..6471
                     /locus_tag="PWN45_000271"
     CDS             4966..6471
                     /locus_tag="PWN45_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDL0622070.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            6583..7794
                     /locus_tag="PWN45_000272"
     CDS             6583..7794
                     /locus_tag="PWN45_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDL0622071.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            7980..10241
                     /locus_tag="PWN45_000273"
     CDS             7980..10241
                     /locus_tag="PWN45_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDL0622072.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            10271..11455
                     /locus_tag="PWN45_000274"
     CDS             10271..11455
                     /locus_tag="PWN45_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDL0622073.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            11769..11876
                     /locus_tag="PWN45_000275"
     CDS             11769..11876
                     /locus_tag="PWN45_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622074.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(12058..13887)
                     /gene="coa"
                     /locus_tag="PWN45_000276"
     CDS             complement(12058..13887)
                     /gene="coa"
                     /locus_tag="PWN45_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDL0622075.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            14077..14421
                     /locus_tag="PWN45_000277"
     CDS             14077..14421
                     /locus_tag="PWN45_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDL0622076.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(14585..16348)
                     /locus_tag="PWN45_000278"
     CDS             complement(14585..16348)
                     /locus_tag="PWN45_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDL0622077.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(16684..17439)
                     /gene="pflA"
                     /locus_tag="PWN45_000279"
     CDS             complement(16684..17439)
                     /gene="pflA"
                     /locus_tag="PWN45_000279"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL0622078.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(17462..19711)
                     /gene="pflB"
                     /locus_tag="PWN45_000280"
     CDS             complement(17462..19711)
                     /gene="pflB"
                     /locus_tag="PWN45_000280"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL0622079.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            20299..21267
                     /locus_tag="PWN45_000281"
     CDS             20299..21267
                     /locus_tag="PWN45_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0622080.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            21264..22820
                     /locus_tag="PWN45_000282"
     CDS             21264..22820
                     /locus_tag="PWN45_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0622081.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            22813..23571
                     /locus_tag="PWN45_000283"
     CDS             22813..23571
                     /locus_tag="PWN45_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0622082.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(23930..25309)
                     /gene="uhpT"
                     /locus_tag="PWN45_000284"
     CDS             complement(23930..25309)
                     /gene="uhpT"
                     /locus_tag="PWN45_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDL0622083.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            25542..26036
                     /locus_tag="PWN45_000285"
     CDS             25542..26036
                     /locus_tag="PWN45_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDL0622084.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(26398..27366)
                     /locus_tag="PWN45_000286"
     CDS             complement(26398..27366)
                     /locus_tag="PWN45_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0622085.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(27421..28461)
                     /locus_tag="PWN45_000287"
     CDS             complement(27421..28461)
                     /locus_tag="PWN45_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0622086.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(28486..29562)
                     /locus_tag="PWN45_000288"
     CDS             complement(28486..29562)
                     /locus_tag="PWN45_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0622087.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(29636..30475)
                     /locus_tag="PWN45_000289"
     CDS             complement(29636..30475)
                     /locus_tag="PWN45_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0622088.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(30477..31745)
                     /locus_tag="PWN45_000290"
     CDS             complement(30477..31745)
                     /locus_tag="PWN45_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0622089.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(31748..33019)
                     /locus_tag="PWN45_000291"
     CDS             complement(31748..33019)
                     /locus_tag="PWN45_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0622090.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(33032..34129)
                     /gene="ugpC"
                     /locus_tag="PWN45_000292"
     CDS             complement(33032..34129)
                     /gene="ugpC"
                     /locus_tag="PWN45_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL0622091.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(34512..35090)
                     /locus_tag="PWN45_000293"
     CDS             complement(34512..35090)
                     /locus_tag="PWN45_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDL0622092.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            35299..35925
                     /locus_tag="PWN45_000294"
     CDS             35299..35925
                     /locus_tag="PWN45_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL0622093.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            36115..36888
                     /locus_tag="PWN45_000295"
     CDS             36115..36888
                     /locus_tag="PWN45_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0622094.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(37186..39192)
                     /gene="ggt"
                     /locus_tag="PWN45_000296"
     CDS             complement(37186..39192)
                     /gene="ggt"
                     /locus_tag="PWN45_000296"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDL0622095.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(39230..41005)
                     /locus_tag="PWN45_000297"
     CDS             complement(39230..41005)
                     /locus_tag="PWN45_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0622096.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(41022..42185)
                     /locus_tag="PWN45_000298"
     CDS             complement(41022..42185)
                     /locus_tag="PWN45_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622097.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(42191..>43477)
                     /locus_tag="PWN45_000299"
     CDS             complement(42191..>43477)
                     /locus_tag="PWN45_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622098.1"
                     /translation="KFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEIEQALHQ
                     KYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFIMIKVIL
                     ALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSILGQNAT
                     KAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIPITIIVA
                     VLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILAFSLKLD
                     ILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAKGLSTTQ
                     VVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIPDIPAVM
                     GGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
BASE COUNT        14077 a         8016 c         7012 g        14372 t
ORIGIN      
        1 aaagactagc aactatattt tcagcagtat tagtgttatc aggatgtggc tcaatgcatt
       61 catctggcaa agacttaaat atttcgttac cgttaaaaac aaagtctatt gcaccttatg
      121 aaacagatgt accagttaag attggtgctg cagaatcatt atttaaaaca aatgatcaag
      181 gaaaaataga aaaagcatta gtgaaatcgt atcatcaacc aaatgataca acgttagata
      241 tagaattaaa agataatatt aaatttcaaa acggtcaaaa attgactgca gaaaaagtga
      301 aatctagcct tgaaaatagc atgaaaaaaa gcgacttggt caaatattca ttaccaatat
      361 catcaattac cgctaaaggt caaaaactga caattaaaac caactccgct taccctgaac
      421 ttgtatctga attagctaat ccttttatgg caatttatga tacagatgct aaatcagatg
      481 ttaatcaaac tcctgttggt acaggccctt accaaataaa agattataag caatctcgaa
      541 aaatatcatt gtcgaatttt aaggactatt ggcaaggtaa accgaaactt gatcatatta
      601 ctgtgacgta ccaagaagac ggcaataatc gcgtcagaaa tttagaatct caaaaagatg
      661 atttaataac tgatgtccca gttaataaag ttcaagacat agaaaataat caaaatttaa
      721 aagtgtcaaa agaatctgga tttagaactt ctttacttat gtataatcat actaataaaa
      781 aaatgactaa atccgttcgt gaagcattag atcatatcat tgatagacaa ggtattgcag
      841 atcatattta tcaaggttat gcgaaacctg caacgagtcc atttaatgat aaaattccat
      901 atattaaaga acctaagtta accaaacaaa atatcgaaca agcaaaaatg ttattagcta
      961 aagatggtta tacaaaagaa caccctttaa aaattaagtt aattacgtat gacggtcgtc
     1021 cagagctatc gaaaattgcg caagtattac aatcagatgc taaaaaagca aatattgaaa
     1081 tcgacattaa aagtgttgat gacatagaag gttacttaaa agaccgttct gcatgggatg
     1141 caacgatgta tagtttcgga acaattcctc gtggagatac aggttatttc tttaatcaag
     1201 catataaaaa agatggtgct attaataaag gagactacaa taatagcaac gtcgatgatt
     1261 taattaatca attgaatcat actgtagatg ttaaggaacg tcacaatatt tctaatgata
     1321 ttattaaatt atcaagtcga gatgtaccaa acagttatat tgcttacaac gatcaaatag
     1381 tcgctgctaa ctctaaggtt aaaaattata aagtaacacc tgaaggtatc tatttaatcg
     1441 attatcgaac aacgattgaa cgataaggga gtgtgggaca ttgatttatg tctcagccct
     1501 gcttcaacta taaaaaccac gccgaagtca tccaatatga ttatggcgtg gtttaatttg
     1561 cttatatgat tgtattaatt tctgtttgat gaatttcaat aaaatgatta ttacatactt
     1621 tcagtgaatc atgttcattt tatataaatc taattgtcac tatttgttta acgtgaaaca
     1681 cttttttaac tccaaaaagt attcctattc cactctttta aagacaaatt ataaaaatag
     1741 cttatgtcac aaacatatga gcaacataag ctttaattat tattcgtcta cttttttctg
     1801 ttgttcttta aattctttct gcagtaaatt cacttgtttt aaacctgccc ccattaaaat
     1861 gaatacaagt atccacacaa taacgattaa aataaatatt ttcaacgtat cactgccaag
     1921 tacagttaaa gatgtatccc aaatgccatg taaaacaacg gctgataaaa agaatattaa
     1981 aaagcgttta tcaaaaatat ctttgaattc aaagccatgc tgttctttcg caataactat
     2041 tgcagcacca acaatcgctg accaaactaa atgaccacca atcgcagtcc acgcacgtgt
     2101 gaagacaata tctaataatg ggacattttc tcctaaagcg aaattcaaaa tataacctgc
     2161 tgattcaaaa actgcgaacc ctgcaccaat agcagcaccg attaataatc cattcaaaat
     2221 cttatttgtt ttcaatttat tgacgaaata aacaataatg agtgcttttc cagtttcttc
     2281 aactaatcct actaaaaatg catcgaaaaa tgttaaagaa ccaaaccttt cgaattgatc
     2341 actaaaaacg acaaatctat ataataccat cgtacttagt aatgaaaata cgccgccaat
     2401 aaagaacatg ataataactt caaaaatgct aatattttta aacgcatttg attcataaaa
     2461 gaagaacaaa cccgataatg gtactgttaa agcccctata aaaatgagac ccggaatcgc
     2521 gttagtgtta ttaaaaattg ctgccatgac ccataatcca ataaatgtta ctgtgaaagc
     2581 gatgaatact cgagaaaaga gccatggctt cccccattct tctgatatgt cacaaattgc
     2641 tggcgtagtt ttagcagtac ccgctataaa tatttcatct gcttcgtttt tagtatgcgg
     2701 cttaaatact tcagaaagca tatcttttaa attaagattt aagctttcat cattgcccgc
     2761 tagtctaccg attgatttgg ttgcattatc aaataaactt cttgcttcgt tttttaaatc
     2821 tgtattttgc gctgtttcat gcgctttagc ttccatttgt ttatcgatag attcattatg
     2881 tatgtcggac gtcttagttg attgtgtact tgctgatgac atcccctctt cacctgtatt
     2941 aggtgcaact gattgcgttt gtgttgtctc ttctttttct gtatttcttc gatgattaag
     3001 accttccata gacttaccta ctttcacttt tcataataaa actattttaa tttaatattg
     3061 atggattttc catattttaa tataaatact ttgttcgatt gggcactttg tatttcatat
     3121 ttaaaaatca aaacctattt aactttttat attccgaaca atatttttat ataagtattt
     3181 taatgatatt caattcatta caaacccttg atattacaat tgttatcgac cgtcaaaatg
     3241 tgtctatact gtgtctatta tcagaataaa aagatatttc taattgattt taaacgtcgt
     3301 tatgttatat ttcttgtaag ggtttacaca aagtgtaaaa acgctatatt ttatcaaatg
     3361 tgatggaggt gacttgcttg aaacaaatca catggcacga cttacaacat atcattaaag
     3421 atggtgatgt gattggttta ccagcattag ctgtagccaa cttacccgcc gaagttctac
     3481 gtgctgtgtt agcgcaacat gacacatatc atacgcccaa tgatttaacg tttatattag
     3541 cgaatgatat tcatagttta ggtgccgcac cggatttaga tgattttata gaacgtcgca
     3601 tgattaaacg tgtcattatg agtattttaa cggcttcttc caaaacggca caagcaatga
     3661 aaaataatga cattgaagct tattttttac cacaaggtat cattgcaact cattatcgtc
     3721 agagtaatca attattacct ggagttatta ctaaaatcgg attaaacaca gctgttgatc
     3781 ctagatacgg tggcggtaaa gtaaatacac gaacaacgga tgatttagtt tcattagtaa
     3841 ccatcaacga tgaaacatac ttacattaca cattccctag cgttgatgtg gcactactga
     3901 gaggaacata cgcagatcaa caaggtaaca tttatttaac tcaagaagcg tacttgagcg
     3961 agtgttatca tgtcgcatta aacgcgaaag ccaatcatgg gaaagttatt gtacaagtta
     4021 aagctttagt tgacgactat caactaaaac cgaatgaagt tgttatccca ggaaatcttg
     4081 tcgattatgt atacgtcaca gaagatgaaa agaatcaccg ccaagtaatt caaagtcatt
     4141 atttaccagc cttgtctgga gaagaacgaa ttgatggaat acctgaaccc gcattacctt
     4201 ttaatagtcg caaattgatt ctccgacgtg ctgctcagtt tttaacttat ggcgatacaa
     4261 ttagcatcgg ttatggcatc aataatgaac tctctaattt attgcatgaa gaatgtgttg
     4321 aacatgatgt gcaaccgatt ttagatgttg gcattttcgg tggattcgtt gggagtcgtg
     4381 aacattttgg tatgaattac aatgcagatg tgcgcatgcc tcatgatcga gcatgggatt
     4441 ttatttataa caatggtgta tcagttgcct atcttagctt tgctgaggtt gatcaatacg
     4501 gcaatgtcaa cgtgtcttac ttcaatgacc gactaaatgg atgtggtggc tttatagaca
     4561 ttacgcaatc tgtaaataaa attatctttt caggtacttt tgtagctggc agtcatgtct
     4621 catgccataa tcaacgatta aacattgaaa ctgaaggaca aaaccagaaa tttgtatcag
     4681 atgtgagcca tatcgacttt aatgcacaat attcacaatc actcgagcaa gaaatctatt
     4741 ttgttactga tcgtgcagta ttcgaactca ccaatcaagg cttgaaacta attgaaattg
     4801 caccaggtct tgatttgcat aaagatattt tgaatcaaat ggcttttaaa ccaattattg
     4861 ctgatcattt aaaattaatt gataccagca tttacaaaga aaaatgggga caacttaaac
     4921 aatcaattca taaagtatga aagtgaagct aaagggggta tactaatgaa tttcgattgg
     4981 ataaaaacgc gttcagactt cgatgatgac aagcctgccg ttattgatca cgcaaaacaa
     5041 acatcttgga cataccaaca actcaatgca cgcgctgata atatggcaca ttatttaaca
     5101 tctcaaggtg ttaaaaaagg cgatgttatc ggtatttttg cgccaaatga tattgcaata
     5161 ttagatttat tgtttgcttg ttttaaaaca ggtgcggttt ttttaccatt gaattggcgg
     5221 cttaacccaa aagaaattgc agccattgtg gaagatgcac aattaaaact gctcttctat
     5281 gctgaaaaac atttaagttc actcaccgat attgaccaaa acttattgca tatggatatt
     5341 gatgtggcgc aatatgatga aatcgtaaat ccagattatc accaaccttt tcaagcaaca
     5401 cctgttgaac cacaagatct tgcagcatta atttatacaa gtggtacaac cggatcacct
     5461 aaaggtgtga tgttctcata tgaatcattt gttcataacg gtgcaaactt agagctgacg
     5521 tataagttca attcaaacta tattacgatt gtatcaacac caatgttcca tgttttaggg
     5581 tttaacgata ctgtattacc agtattaatg tcaggtggca cacttattct tcaacgttac
     5641 tttaacggcg aagaattgaa tgacatgatt gcacaatatc accctacatt tattatcatg
     5701 attccgacaa tgtattatag tacgctacgt gctagtaatt ttaatcctga aaattttaga
     5761 gctatggatt atatcatcca aggtggttca caaccattac caagtatcca agcagctttt
     5821 aaacaatatg gcataaacat tattaacggc tatggtttaa ctgaagcacc tcttgtactt
     5881 gttaatacac cggaaaattc aaaacgtaag ccaatgagta ttggtaaagc agtcatgttc
     5941 gttgatgcac gtatccttga tgataacggt gaggaagtac ctactggtga gattggcgaa
     6001 cttgcaatta aggctaaaaa tgtcacgcca ggatattgga ataaaccagc agagactgcc
     6061 aaagcatttc atggtcgata tttattaact ggtgacttag cgaagatgga caacgatggc
     6121 gatatattta ttattgaccg caaaaaagaa ttaatcataa ctggtggcga aaatgtctta
     6181 ccatccgaag tcgaaaatgc tttagctgag catccactag tagaccggtg tgtggtcgtt
     6241 ggctatgatc atccaaaata tggtgaatca attgctgcag ccattatact tcgcgaagat
     6301 gaacctcatt acgctgaaat tttaaatcaa catatgcgaa gtcgtttagc aggttataaa
     6361 gtcccaagaa tgtatgtacc agtgacacat atgccgttaa acagtacgca gaaaccagat
     6421 aaacttgcga ttcgacaaat gatgaatgac aaagtctcgc aaacacttta aaggtgataa
     6481 aaatttttga catttagtgt aagcgtttac aaataaagcg tgttgttttt gaattaaatg
     6541 catttcacat tagtattcat attattttta ggaggaattt atatgacatt tgaaaaagaa
     6601 acggtcttaa aaacattatt tcctgaagat gtacttagta ttgctaaagg tttaacagac
     6661 ggtgaagtcg aatttttaca acaagtagat tcattgctag aaagtaagta ccgtgaaaat
     6721 attaatcaac attggataga cgctactgta cccgaggact attttaaaga tctgggagaa
     6781 ttaaattatt ttaacaatcc attactttac aaggatcgtc caaacgccaa aatgcctagt
     6841 caactatttc agtttttcat gtcttaccta ctcgcgcgat ttgatatttc cttagctacc
     6901 ctactcggtg ttcaccaagg tttagggcat aacactttct atttcggagg tagcaaagaa
     6961 caaattgcga aatatgtacc taaattacag tcacatgaac tgcgtacatg ctttgcttta
     7021 actgaaccag aacacggttc ggacgttgcg ggaggtcttg aaacagtcgc tgaacgccaa
     7081 ggcgatactt gggttatcaa tggtgaaaag aaatggattg gtggtgcaca tgtatctgat
     7141 gtcattccag tattcgcagt aaataaagaa actggcaaac cccattgctt tgtagtcaga
     7201 ccagaacaag atggcgtcga tattgaagtc attgataata aaatcgcact tcgcattgtt
     7261 cctaacgccc taattaaatt aactaatgtc aaagtagatg aagcggatcg cttacaaaac
     7321 ataacaagct ttaaagatat tgccaaaatt ctttattcaa cgagagcagg cgttgcttat
     7381 atggctacag gtggtatggc tggcgcttta cgtgccacat tagattatgt cactgagcgt
     7441 aagcaattcg gcaaaccaat tagtaaatat cagttaatac aagaaaagct agcaatgatg
     7501 caaggtaatt tagctcaagc aatggcaaca tgtgctcaat tagctaatat gcaagcacat
     7561 ggtgaatatg acgaggttgc aacttcaacg gcgaagatga tgaatgcctt acgtttgcgt
     7621 gagacagtag ctatgggccg cggtattaca ggtggtaatg gcatactagc tgacgattat
     7681 gatattgcac gtttcttctc tgatgcagaa gcgatttaca cgtacgaagg tacacatgaa
     7741 attaatgcct tagtaattgg acgcgctttg actggagatt ctgctttcgt ataaatagca
     7801 aataattata tgagatgcat taatttcact aaaaaagact tattttaagc ataaagcttt
     7861 ttccttaaat aagaggctaa gatgactgtc aaagatactt aattaatttt ataaaatagc
     7921 aacgttattc caattatctt aatggttatc ttatcctcaa ctaaattgga ggaatcacta
     7981 tgacaattaa taaagtaacc gttcttggcg caggcacaat gggcgctcaa ctggcagcac
     8041 tttttgtgaa tgctggactt aaagtaaaac tattagatat tgtagtggac aaaaacgatc
     8101 caaatctcat tgcgaaaaaa tcttacgata aaattacaga taagaaacgg ccgctactat
     8161 tcgacttaaa tctagcgagt catttaacat atggtaattt tgatgatgac ttggtaaatg
     8221 atgatgctga tttatatatc gaagcagtca aagaagatat tgaaattaag catgctgttt
     8281 ggcaacaagt tctacaacat gctaaagaag atgctttatt cgctacaaat acatcaggta
     8341 ttccaattaa tgcgattgct caagcattta acgagaagga tcaagaacga ttctttggtc
     8401 tacatttctt taacccacca cgtattatga aattagtgga gttaatacct acgtcacaca
     8461 cgaaggaatc tattatatta gatgtaaaaa atttcgcgca aaatgtgtta ggtaaaggtg
     8521 tcattgtcgt caatgatgtg cctggctttg tcgcaaatag agtcggcacg caaacaatga
     8581 atgatattat gtatcgcgcc gagcaacaca agataagcat tgtagatgtg gatgctttaa
     8641 ctgggcaagc gattggtcgt cctaaaacag gtacatatgc gctatctgac ctagtcggtt
     8701 tagatattgc agtgtctgta attaaaggca tgcaacaagt acctgaagaa acaccttatt
     8761 ttcatgatgt caaaattgta aatacgttgt ttgacaatgg cgcactcgga cgtaaaacga
     8821 aacaaggatt ttacaaaaag gataaagaaa ctaaagctcg acttgtttac gatgttgaaa
     8881 aacaagatta tgtacctgta tcgcaaccac aattaccaat tttaaatgaa tttaataaag
     8941 acttagtgca taaccttgat accatattca atgcgcaaga cgaagcggga ctatttttat
     9001 gggagacatt acgtaataat ttctattact ctgctatcaa tgtacctaaa gctaccgatg
     9061 atttccgaga catagaccgt gcgcttgtct gggggttcaa ctggaaactt ggtccattcc
     9121 aattatggga tgcaatggga tacgaacgtg ttaaaacacg tatggaagac gaacttggag
     9181 acttaccaca atggattagt gatttagatg gtggctttta taaacaagat gagaccattg
     9241 aatatgcaac acctatttct cacttcgtaa aagatgaact ttgggataaa ggtgatgcca
     9301 aactttccgt aactcatgat gatcaactgt tactgaaatt acaaagtaaa aataatgtca
     9361 ttaccgatga attcaacgat gcgttagttg atgcgattga tttactggaa aatgaccatt
     9421 acacaagtat ggttatttat gcagatggta acaatttcag tgtgggtgct aaccttttct
     9481 taatgaaaaa ggcgcatgaa gacggtcttg tagatgatgt cgttgcacaa tcaattgata
     9541 aattacatta tagctttaat cgtttgaagt atagtttgaa accagtagtc acagctgttc
     9601 aaggtcgtgc cttaggcggt ggctgtgagc ttgtacttta ctcacctatt gttgtcgctg
     9661 caagtgaaac atatatcggt cttgttgaag caggtgttgg cttattaccg agtggcggtg
     9721 gccttgcaga aatggctgat cgcatattac gcacatcgca taagtttgat gacaaacaag
     9781 cttccatgac aaaagtactg acgaatatcg catttgcgaa agtctctaca aatgcctttg
     9841 aggcacgtcg ttatggttat ttacgtgata cagatacgat tattttcaat acagcacaac
     9901 gtgtcgaagt tgcgctcaaa cgtgcgaaat atgaagcaga aacaaactat attccgaatc
     9961 ctagacatca atatatcgct ttaggtgaag acttcaaagc attgatccaa ggacaattag
    10021 atgcgcaaag acggggtcat tttattagcg accatgatta tcatattgcc ttaaatatcg
    10081 ccacaatttt agcgggtggt gatttaccaa gaaatacatt tatcaatcaa cgttacattc
    10141 aatcgttgga gaaaattggc tttattgact tactaaaatc taaaaaatca tatgaaagaa
    10201 ttgcacatat gttaaaaact ggtaagccat tacgtaatta aaagatagtc attaagagag
    10261 gatgataacc atgcaagaag catacattgt agcttatggg cgttcagccg cagcgaaagc
    10321 aaagcaaggc gcattattcc acgaaagacc tgatgatgtc gcagccaaag tattacaagg
    10381 cgtattgaaa cgtattgacg gaaaattcaa taagaatatg attgaagatg tcattgttgg
    10441 tacggctttt ccagaaggat tacaaggcca aaacattgca cgaacgattg cattgcgtgc
    10501 gggattatct gacacggtac cgggtcaaac agtgaatcgc tactgctcat caggattaca
    10561 aaccatcgcg attgcagcca atcaaattat ggctggtcaa ggagatatac ttgtagctgg
    10621 tggcgttgaa ttgatgagtg ccgtaccaat gggtggcaac gagcccacaa acaatccaac
    10681 cttacaatat gatgatatag gtgcgtcata tcctatgggt ttaactgctg aaaatgtagc
    10741 atcccaattt gacgtatcac gtgaagatca agatgcttat gctgtcagaa gtcatcaacg
    10801 tgcctatgac gcacaacgtg atggtcggtt caaagatgaa attattccaa tacaagtaaa
    10861 ctcagttgaa tatacaaacg caggaccaaa agtacacaca aatatctttg accaagatga
    10921 atttatacgc cctgacacca cgatggaggc attagccaaa ttacgtacag tatttaaagc
    10981 tgacggcact gtgactgcag gaacatctgc cccactttct gatggtgcag gatttgtagt
    11041 tttaatgtct ggagataaag tgaaagaact cggcgtgaca cctattgcac gattcgttgg
    11101 ttttaaggca gtaggcgttg acccgaaaat tatgggtatt gggcctgcat atgcgattcc
    11161 tgaagtattg tcactcagca atctatctgt tgaagacatt gatttgatcg aattgaacga
    11221 agcatttgct tctcaaacga ttgcatctat taaagaagta ggtctagata tatcacgtac
    11281 gaatgtgaat ggtggcgcta ttgctttagg tcatccatta ggtgctacag gcgcaatgtt
    11341 aaccgcgcgt ttacttaatg aaatgggtag acgtcccgat agccgttacg gcatggttac
    11401 gatgtgtatt ggtgtcggca tgggtgcagc tgctatattt gaatatgtgc gttagaatgg
    11461 ttgattttgg atgaagcgga ttcgttttgt tattgaatga agtaggctga agttgaagcc
    11521 agttgaagtt gaagcgggtt gaagcaattt cgttttatta atgaagctgt gtgaaatata
    11581 gtgattgaac aaaaaagtgg tttaatggga tggtggttat ttccgtttta gaatttaaca
    11641 tttacacgtc taattttaat cattgtttta aattttatga atcgaagccc tttgatttaa
    11701 taatatttgc taatgctagt aacttatctg attgttcatg tttaaaataa agaaaaccac
    11761 tcacatcagt gtgtgttcga actagacttg taagttccag ttcggcacga ctttctaaag
    11821 caattattat tgctgtgatt gtcgtatatc acttagatgt gcgtggttta ttttaatagg
    11881 ttagtaatat attaggtcat gttatgttta agactataat gaataaataa tttagaaata
    11941 tgcttccgat tgttcgatgc tttaattcag ttagaagcat catagaatgc atgattactg
    12001 ttgtaaagat acgtaatgtt ttgtattgac tgtatgtctt tggatagagt tacaaactta
    12061 ttttgttact ctaggcccat atgtcgcagt accatctgca tgtgttgtta cattgtatgc
    12121 atttgtttta cttggcttct tgtatgtcgg gcgagctccg tatgacactt gaccgtttgc
    12181 atgtgttgtt acgttgtatg catttgtttt gcttggcttg ttttgtgttg ggcgagcgcc
    12241 atatgatact tggccgtttc catgtgttgt tacgttatat gcgtttgttt tgcttggctt
    12301 gttttgtgtc ggacgagctc cgtatgatac ttgaccattt gcatgtgttg ttacgttata
    12361 tgcatttgtt tctgatggct tattgaatct tggtctcgct tcatatccaa atgttccatc
    12421 gttgtattca cggatacctg taccagcatc tctatattta acatatttag gtgttttgtt
    12481 aaattgcggt ctcggaccat attgagaagc ttctgttgtt tcagttgctt gaggtttaac
    12541 ttcaatatca cttgattctc cttgagtacc ttttaacgtt gattcagtac cttgtggttt
    12601 tatttcaagt ttagatgagc taccttcaag accttctaaa atagggttcg ttaacggtgg
    12661 gtttgtataa ttattgctta atgatgggcc gctttgttcc attgttagaa aatcgggacc
    12721 ttgaacgatt tcaccttgta ccgttttatt ttccatcgtt ggatattccg gaccttttac
    12781 aatttcacct gtaattgtgc cctgtggaat tttaactaat ggttgtgcaa ctggttgtgt
    12841 tgtttcttca gctttaccag ccgtagtttt aacctcttgt tggttatcaa ctttaggtgc
    12901 ttgaggttct tcaactttct tctcttcttt tactactggc gattttgttt cagtttctcc
    12961 gtattttttg acagttttct ttttccaaga atcatctgct tctttaactg cttttttcgt
    13021 ttcttcaact aatttatcaa aattaggttt attatcacta tttgttttat agttatgtgt
    13081 tgtaggatta tatttcgtta tagatttcgg tctattttgt ttagtttcca taaagaaatc
    13141 atcaataatt gaatttaagt catcaatcat ttctttttta atacgttcat ttgtaatttt
    13201 atgtggattg tctgtatctc caaggattaa gtccagtttt gctcgtaact ctttcgcgtg
    13261 ctccccataa tccttatcac cataatatga tacaactaat gtatcaattt cagatacgag
    13321 atcgtatact tccttagttg ctttatcttc ttctgctgca ttaaaagttt tcaagtctga
    13381 attcttatcc ttaatatctt taacttctct gtgaaaatca tccagtgctc tctttaatgc
    13441 atcctgtagt tcattgtatt ctttcatcga aagttcttct aaattatatt tatgaaaatt
    13501 agccattttt aaatctgtac gaggattttc ttttttataa tttgcatacc attgtttata
    13561 atcttcatat tgagatttct ttctctccaa aagatattga tcttccctta ataccttttc
    13621 caacaaccta tctttagctt ctttataaat attatctcca tattcatatt tattagttaa
    13681 tcctatagca taaattatat agtcttccaa ataatataga gctgaattta aatatctgct
    13741 atctattaat gtcccatttt tactcccagc attaacttgt gatttcccac tataatcctt
    13801 tgttactatc gcatctgctt tgttatccca tgtaaataag ctagatgcaa ctgctaatgc
    13861 gcctagcgaa attatttgct ttttcataat tttttaattc ctccaaaatg taattgccca
    13921 atctacatta aagaaacaaa atattaaaag acattaacta tatattaact agaataaaca
    13981 aagcattaac tatctttttg ttatagttaa ttagctttgc aatatacaat agtgtataat
    14041 ttttttgatt aatgtacatc aaaggagtaa caaagcatga caacacaaat gaaaatcaaa
    14101 acatatttag ttgctggtat taaagcggcg ctccttgata cgactggtat taaattagca
    14161 agcaaatctg aaactacatc acatacgtat caacatcaag cgcttgtaga tcaattacat
    14221 gaattaatag caaacactga cttaaataaa ttatcgtacc taaatttaga tgcgtttcaa
    14281 aaacgcgata ttttagctgc gcactatatt gcaaaatccg ctatacgcac taaaaatttg
    14341 gatcaaatga ctaaagcgaa acaaagatta gaaagtattt acaattcaat ttctaaccct
    14401 ttgcattcac aaaacaatta ataattcacc aataaatcat gtaagtgttt gtgacgccaa
    14461 attgccatac aaatacttgt catatgaata taaacgaatg aaacgattgc ctatcccata
    14521 gatggcaaca ttaaaaagac ctctgaaggt atcatcttga taactttaga ggtctttgtt
    14581 tatattattc aaacaaaatt cttatagaac gatcgaagta tgtttcgtct ttcttttctt
    14641 ctttaatctg atcagctaat gctgggtcat ctgtgataat accatcaaca ttggtttgta
    14701 agtatttcgt taaatcttct tcgccgttaa tagtccaagt atagacttct ttattttcca
    14761 agtgcgcttg attaacaagt cttggcgaat aagaaaaatc ttcgatgaca aagaaatcta
    14821 atgatgtttc tttaaaatga ccaaactgca acggaatgat ataaccacac ttgagatatg
    14881 gcgcttcttt tttcaactta gtcatcacat cataatccaa agacatcaca cgatattgat
    14941 gttcaacacc atgctttttc aaaatatcaa taacacgttg tgtataatct gctggttctt
    15001 taccatgtgg ctttaactct actagtagct tcacatttga ttgtttagcc gtttcaataa
    15061 attcgtctaa ggatacaaat tttgcttcat gtccattttg acgcattttc aaaccgacga
    15121 tatctttgaa attagattca gaaatatttt tattaacacc tgttaaacgt ttcaaattgt
    15181 tatcatgact aacaacaaat tgtttatctt tcgtcataat tgtatctaac tcaacgtatt
    15241 cgacattcgc ttttgcagca gctttcaatg acggaataga attttcaacg cctttatctt
    15301 cgaaaccacg atggccaata atggagatat ttgtattgat agtattattg taaagtaagt
    15361 acatgttata accgataaaa catgtcactg caagcaccat tgaaattata aagaacctag
    15421 acttccgttt cggttttgga tatttaaatt ctaagcccgg ttggtctaat acattctctt
    15481 gttttaagtg cagtactaac acactgatta atgataattt cgtaaataaa taatagaaga
    15541 acaatgcgct tttcaataca acaaataaaa ttgatgagac taaaaactta tctccttctt
    15601 catctacaca aatagcaaga tatgttgctc ctgaaataat taatgttaaa atcgcaccaa
    15661 tgatgagttc taatataact atttctataa caagccgaaa cttatttcgc ttcgtaattt
    15721 gccaacttag tctcatattt ttaaataacg actggcggtt taaaatcgtt aacggtagag
    15781 taaatattaa tttaaaattt aatataaata cagcaatcat aaaggtaccg taaatgatta
    15841 tacctttcgt cgttttcata agttcttccg ttaaaaattt aggtatgtaa atattttttg
    15901 ttaatactga acttagtcct aggttggcaa tgggtatcat taacattaaa taaatgacaa
    15961 agaaaataac tggtacacct atgagtttac gcacatttac aaaggcattt ttaaaaatgg
    16021 atttaaatgt aataatctgt cgatcaaagc cggcataaac catataaact aacaatgaaa
    16081 actctacata aatcagaaag gcaacactta atatgaatat aataagaagt atcacactgg
    16141 cgggatgact aacgatttcc gtccaattgt taatcgtaag ttggctttgc ccagctactt
    16201 ttaacatcat attaaatagt aaaattaagt atgtactact aataaaaatc atgattaact
    16261 gcaatagtaa ggcattaatg ctaaaacgcc ctttattttg atacagtaat ttaaatactg
    16321 cccatatatc tttactaatt ctcttcataa tcacgctccg cattgcttta atattaagtt
    16381 tcatcttaat atttttcatt actcagggtc aataaaaatt tgaaaagact catattcata
    16441 tgcaagtagc aaataataac ccattcaaca tcagctaaat gatgatattg gaaccccacc
    16501 tttaacaaga catcacattc tttatcagca tagctactta caaaaacgtc ttccttcaac
    16561 cattgtagaa gttgtctcat atacttacta cgtcttgcta atactcaata ctcaattgaa
    16621 aaagaagcat atgccccttc actcttgaag ttgcatatgc tttcttttcg gtctgaattg
    16681 tatttataat tcaacgggaa ttttcccttt gaagttaaca taacggtagg ctgctttaac
    16741 agcttcatca tcgggcgctt cgacatcttc taattcatat gcaatgccca atgttttcca
    16801 cttatgaaca cctaactgat gatatggcag aatttcaaac ttttcgacgt tatcaagaga
    16861 attaataaat tcccctagtt taattaaatc gtctttatca tcagaataac caggcacaag
    16921 gacatgtcga atccatacag gttgtttcat atctgacagt ttgcgcgcga agttaaggat
    16981 gtgtgtatta ggctttcctg tcaatctaat atgtttgtca ttatcaatat gttttatatc
    17041 taataatatc aagtctgtat gtttttgtaa ttcttcaaaa tgcctttgaa atgcttttgt
    17101 atcattagca catccagccg atgtgtctaa gcaagtgtgc acaccatttt cttttaattc
    17161 tgcaaataat ttttctaaga atggcatttg taacaatggt tcgccaccac tgactgttac
    17221 accgccaccc gatgcatcaa agtatggttt gtatggtaat atttcattca ccatttcatc
    17281 aactgtgact tctcttgatg gctcactaat tttccaagta tctggattgt ggcaatacaa
    17341 gcatctaagt aagcatcctt gtgtaaataa tatatatctt aatcccggtc catcgacagt
    17401 acctaaactt tcgacagaat gtaagtgtcc cttaagcata gtgctcccac cttaaatttt
    17461 gttacatact ttcatggaat gtacgagaaa ttacatctaa ttgttgttca cgtgttaatt
    17521 taatgaagtt aacagcgtaa ccagatacac ggattgttaa ctgtggatat tcttctggat
    17581 gttccattgc atctattaat gtttcacggt taaatacgtt aatatttaag tggtgaccac
    17641 attgcattgc gtaaccatct aacatactag ttaagttacg gttttgatct tctggttctt
    17701 tacctaatga ttttggtacg atactgaatg tatttgaaat accatcttta cagcaatcgt
    17761 aagggatctt agctacagaa cttaatgaag ataatgcacc tttttggtca cggccatgca
    17821 ttgggtttgc acctggagca aatggttcgc cagctttacg tccgtctggt gtgttaccag
    17881 ttttcttacc gtatacaacg tttgaagtaa ttgttaatac actcattgta tgttctgaat
    17941 cacgatatgt tttatgacta cgtaatttag tcatgaagcg ttctactaaa tcaactgcaa
    18001 tatcatctac acggtcgtca ttgttaccgt atttagggaa gtcgccttcg atttcaaagt
    18061 ctactacaag accttcttcg ttacgaattg gtttaacttg tgcatattta attgcagata
    18121 atgagtcagc tgctactgat aaaccagcga tacctgttgc cattgtacgt acaatttctg
    18181 tatcatgtaa tgccatttca atacgttcat agctgtattt atcgtgcatg tagtgaataa
    18241 catttaatga gttaatgtaa acacctgcta gccaatccat catttgatca aatttcttga
    18301 atacttcgtc atattctaat acttcgctgt taataccttc gaagtttgga ccaacttgtg
    18361 caccagattt ttcatcttta ccaccattga tagcgtaaag taatgtttta gctaagttcg
    18421 cacgtgcacc gaagaattgc atttgtttac caattgtcat cgctgataca caacatgcga
    18481 taccatagtc atcgccatag ctttcacgca taatgtcatc attttcatat tggatagaac
    18541 ttgttttaat actcattttt gcacagtatg ttttgaagtt gtcaggtaaa cgtactgacc
    18601 ataatactgt taagtttggt tctggagctg gacctaagtt atctaatgag tgtaagaaac
    18661 ggaatgagtt tttcgtaaca agtggacgtc cgtcaatacc tacaccaccg atagattcag
    18721 ttacccaagt tgggtctcca gagaataatt cattgtaatc aggtgtacga gcaaatttaa
    18781 caatacgtaa tttcatgatg aagtggtcaa taatttcttg aacttcgctt tcagtaataa
    18841 cgcctgcttt aaggtcacgt tcagcataga tatctaagaa tgttgatgta cgacctaaac
    18901 tcattgctgc accgttttgt tctttaattg cagcaaggta tgctaagtat aaccattgaa
    18961 ctgcttcttt gaagttttct gctggacggc ttaaatcgaa accatatttt tgtccaagtt
    19021 cttttaattc ttttaatgca cgatattgtt ctgataattc ttcacgtaaa cgaattacat
    19081 cttctgacat ttctgtagac atcgtgttga agtcgtgcat tttttcttcc attaagaaat
    19141 ctacaccata taaagctaca cgacgatagt caccgataat acgtccacgt ccgtatgcat
    19201 caggtaaacc agtgattaca cctgctttac ggcagttcaa catttctcta gaatatgcat
    19261 cgaatacacc ttggttatgt gttttacgat aatctgtaaa gattttttca gtttcttcgt
    19321 ctaattcgta accgtaagct tcacaagctg ctttcgccat acgaatacca ccgaatggtt
    19381 gcattgaacg tttgaatggc ttttcagttt gtacacctac aattgtttct aaatctttgt
    19441 ctaaataacc agcatcatga gatgtgattg ttgaagctac tttcgtgtcc atatcccaca
    19501 tgccgccacg ttcacgttct tctttcgata actgcattac ttgttcccaa agtttagaag
    19561 ttgcttctgt tggtcctgct aaaaatgaat cattaccttc ataaagagtg tagtttaatt
    19621 ggataaactc tcttacatct acgtgtctgt tccatcttcc atttttaaat ccttgccaag
    19681 ctgttgcatg atttttattt gtttctaaca tactaaacgc ctccatctgt ataatgttgt
    19741 gaatttgaaa tgctatgtta acgttttcat agtcattgta gcatgtttgt tgtgattttt
    19801 ttcacataaa ttatgtacaa ttttcatcaa atgtatgtca aaacatagaa actgtactaa
    19861 tttgtgtaac agtaatatgc taattgcatt ctgttacaca acagttataa tgttcactat
    19921 tttcattgct ttgaaagtta atttaggagt atatatttgt ttataaaaaa tgattgttga
    19981 taagcgttaa atcaacactt agaaagattt acttttatat atgaagagac tggattaaat
    20041 acttttattg acgtaaaaat tcacttttga accgttcaat atcttgccga tttttatata
    20101 acagctacaa ataaaatata acagtttgat tttacagcct cggtaaatcg tcttgacaaa
    20161 caaaaatttt gtgctatcac aacatttgca acgtcttaac aagtcatcta taaacatttc
    20221 taaatattta acattactta tgcgtcattt attgctaaaa ttattgtatt aaaatataca
    20281 tagaattgat gggatatcat gaaatcaaaa atttatatct tgctattatt tctcattttt
    20341 ttatcagcat gcgctaatac gcgtcactct gaatccgata aaaatgtatt aacagtttat
    20401 tctccgtatc aatcaaactt gattcgtcca attttaaatg aatttgaaaa acaagagcat
    20461 gtcaaaattg aaattaaaca cggatctact caagtactgc tttcaaactt gcataacgaa
    20521 gatttttcgg agcgtggtga tgtctttatg ggtggtgtgt tgtcagaaac aattgatcat
    20581 ccagaagatt ttgttcccta tcaagataca tctgtaacac agcaattaga ggattatcgc
    20641 tcgaacaata aatatgttac tagttttcta ttaatgccaa cagttatagt agtgaattca
    20701 gatttacaag gagatattaa gattcgaggt tatcaagatt tattacaacc tatacttaaa
    20761 ggtaaaattg cgtactcaaa tccaaataca acaacgacag gctatcaaca tatgcgtgct
    20821 atttatagca tgcatcatcg agtaagtgat gtgcatcaat tccaaaacca tgcgatgcaa
    20881 ctgtcaaaga cgtctaaagt cattgaagat gttgcaaaag gtaaatatta cgcaggtcta
    20941 agctacgaac aagatgcacg cacatggaaa aacaaaggtt atcctgtatc aatcgtttat
    21001 ccaattgaag gaacaatgtt aaatgttgat ggtattgctt tagttaaaaa tgcacaccct
    21061 catcctaagc gtaaaaagtt agtgcaatat ttaacaagcc gctcagtaca acaacgatta
    21121 gttgcagagt tcgatgccaa gtcaattcga aaggatgtta gtgaacaaag cgatcagtct
    21181 atcgaaaatc ttaagaacat acctttaata ccgaaatcta aattaccgga tattccacat
    21241 cataaatttt tggagatgat tcaatgacgg catacaaacc ttatagacat caattaagac
    21301 gatcgttatt tgcgtctact atttttccag tatttttagt cattattatt ggactagtaa
    21361 gcttctatgc catctatatt tggattgagc atcgtacgat tcatcaacat gttgatgaaa
    21421 gtcagtcatc tttacatcat actgaaaagc agattcagac atttattaca cagcataata
    21481 attcgtttca agagctcgat ttaacaaatc atcatgatgt gaccgctaca aaacgtgaat
    21541 tattaaaatt aatacaccaa cagcccgcaa cactttatta tgaattatct ggaccaaatc
    21601 agtttataac caataattat gaacatttaa acactaaaaa tatgtattta ttttcaacgc
    21661 atcagcttaa attcaaaaat agtacatata tgcttaaaat ttatatggct aacaccccac
    21721 gtttaagtga aattaagaaa gataaccgac aattcgcact tatcgttgat caatatgaca
    21781 atattttata cgccaacgat gaccgtttta ctataggtga aaagtataga ccacaacaat
    21841 tcggatttat gaatgaatct gtaaaattaa accatgctga ccatcgtctc attatttata
    21901 aagatataca tgaaaacatt gaagatggta tcacgttgtt aattgttatg gccgtagttc
    21961 ttgttttact agtaatattt ggtttcatta gcgcagataa tatggctaaa cgacaaacaa
    22021 aagatatcga aacgattatt caaaaaattt actatgccaa aaatcgtcat ctaggtacat
    22081 atacgccttt aaaaaacaac agtgaactag aagaaattaa taattatatt tacgatttat
    22141 ttgaatcaaa tgagcaactg atccattcta tcgagcatac agaacgtcgc ttgcgtgata
    22201 ttcagttaaa ggaaatagaa cgccaattcc agcctcattt cctattcaat acgatgcaaa
    22261 caatacaata tttaattacg ttatctccga aattagcaca aactgtggtc caacaattat
    22321 cgcaaatgtt acgttactca ttacgtacaa actcacatac agtggaatta aacgaagagt
    22381 tgaactacat tgaacagtat gttgcaatac aaaacatccg ttttgatgac atgattaagc
    22441 tccatattga aagttctgaa gaagcgcgtc accagacaat tggtaaaatg atgcttcagc
    22501 cactcataga aaatgctatt aaacatggta gagatactga gtcattggac attactatac
    22561 gcctgacatt ggcacgtcag aatttacatg ttttagtttg cgacaatggt atcgggatgt
    22621 cgtcatctcg cctgcaatat gtacgccaat ctcttaataa tgatgtattt gatactaagc
    22681 atttgggatt aaatcattta cacaataaag caatgattca atatggttca catgcacgac
    22741 tccatatttt ttcaaagcgt aatcaaggta cgttaatttg ttataaaata ccactttcaa
    22801 gggggaatgt agatgtttaa ggtagttatt tgtgatgatg aacgaattat tcgagaagga
    22861 ttaaagcaaa tcattccttg gggagactat catttcaata caatatacac tgctaaagat
    22921 ggtgttgaag cattatcact aattcaacaa catcagccag aattagtaat tactgatatt
    22981 cgtatgccac gtaaaaatgg cgtcgattta ctcaatgata ttgcgcatct agattgtaat
    23041 gtcatcatat tatcgagtta cgatgacttt gaatatatga aagccggcat tcaacatcat
    23101 gttcttgatt atttattaaa accagtagac catgcacaat tagaagttat tttaggaaga
    23161 cttgtccgta ccttattaga gcaacaatca cagaacggcc gtagcttagc atcttgtcat
    23221 gatgccttcc aaccactatt aaaagtagaa tacgatgatt attatgtaaa ccagattgtg
    23281 gatcaaatta agcaatctta tcaaacgaaa gtgactgttt cagatttaat tcaacatatc
    23341 gatgttagtg aatcgtatgc gatgcgaaca tttaaagatc atgttggtat tacaattgtc
    23401 gattatttaa atcgctatcg tattttacaa tctctgcaat tgttagatcg acattacaaa
    23461 cactatgaaa ttgcagacaa agtaggcttt tcagagtata aaatgtttag ctatcatttt
    23521 aaaaaatatt tacaaatgtc gccaagtgat tattgtaagc aagcaaaata gattagcaca
    23581 taactaatga ttatggcgcc tccattttgt tctatgaaaa agcggactga tagagaagtt
    23641 ttcacttttc tttcagtccg ctttttagag agtttaaata taaaacaata ctccctacaa
    23701 tattgacacc gctatatgtc tttattgtaa ggagtaaatt cattgagtca ccctaagtcg
    23761 ggcaaataag gatacttcat aacagggaat tatgatctgc cccgagacat agagatgata
    23821 tatttgcaag ggtagaagca gtatctttca tctcttcacg cacatgccgt cggggcagga
    23881 ttgccaacta tattgcaata gattggcgat acttttaatc cgatttatat taaattttta
    23941 aacttctaat tttcttttct tcatagaaag caacgattcc taatagaatc atgcctagga
    24001 ataatgcaac atagaagacg atgaaaacat ctgtccatcc acttaatgta tatccaaaga
    24061 tgtttaaacc gttacgtgtt ggatcagcaa tagccgccaa accaactttc gccattgagt
    24121 caccgaatag atacgcgaat gaacctgtca ttccgtttgc tacactgatg gcatttttag
    24181 gaacaaaacc agtcaatgat acaccaatta ataattgcgg accaaagatt aacgcaccta
    24241 atgcaaacaa tgaaatgtta accatcatga cacttgtagc atttgtgtag aataagacaa
    24301 caaatgtaat cataaacata cagccaatag ctacaattgc acgacgacct tttaataagt
    24361 ctgatacgta gccccataat aaacttgcaa ctaatgcacc aatttcaaag tagaatatcg
    24421 tattaactgc atcgccttta ctaaagtgta aatgctctga cacatataac ggtgcccagt
    24481 tatcaatacc gattcgtaca atgtatacaa agacgtttga aacacataga atccatataa
    24541 caggatttcc caggatatat tttttaaaga tctcccattt cgtcatacct tgagaatcaa
    24601 tattttcttt atcgaccggc tcttcccaaa tttcttcagc acgattccat cctaattctt
    24661 ccggatcatc ttttccgata aataatgttg cgataccaat aagtaatgca atcaccgatg
    24721 ggaaaatgaa catccctata acatttccat ggaagaatac attagcaccc caaagtgcaa
    24781 caccacctgc tatggcacca ccgatattat gtgatgtatt ccagaatcct aagtatcggc
    24841 cacgtttcgt tcttggcgcc catcttgaaa tcgttgaata acttgcaggt ccaccaactg
    24901 attggaacac cccgttaagt ccccaaagta caattaataa tcccattaca gaaccaaagt
    24961 aacttaaaac aaatcccata attaaaactg taatcgcaga taagataagt aagaacgaga
    25021 taatacgttt tgtgttacgt ccatcgacaa aatatccaag taatgttttt cctaaaccgt
    25081 acgtgatact aaatgctaat ccgatataac caagttctaa tgtagataat ccaatttcct
    25141 cttttaaaaa cggttgtgcc gccttaaagt tgtttcgaat taaatacata gccatataaa
    25201 caaagaacac tacgaagaaa gcttgcatga agtttctaag ccataattta cgttgtaccg
    25261 ataatggaat gcctttgttc ggaatcttat ggatatcaaa aaagttcatc tctgtcacct
    25321 caatcatttt cgttaagcca aatatatcac gtcggtgtaa gcgcttaata tccttaaaat
    25381 tattaaatta tccaaaatac tgaacataaa cacatactta gaaacatatt tatgtactaa
    25441 aattaataaa tagttaatag tatttgacaa gctttatcta taaaatactt tatgttgtca
    25501 aaatcaacgc ttatacatat gacataatga cggaggacat catgattcta tctatattac
    25561 ttatcttttt ctgtatcaga cttgtcagct taaagatatc tattaatcac tcaaaacaat
    25621 taaaagcaga cggtgcagtt gaatatggcg ttaaaaattc gaagttttta gcaataacac
    25681 acgttttaat ttatgtattg gctggtgtag aggcatttat taataaagat acatttagct
    25741 ttgcaaatgg tattggctta gttatattaa tctttgctta tatcatgtta tttatggtta
    25801 ttaaaacttt aggtggtatt tggacattaa aattattcat tttacctaac catcctatta
    25861 ttaaatctgg attatataaa attacaaaac acccaaatta cttcttaaac atcattcctg
    25921 aattaatcgg tgtattatta ttaacacacg caacatatac aactatttta ttagtaccgt
    25981 atgcgtattt cttatatgta cgtattaaac aagaagagaa attaatgaat atttaaaaag
    26041 atgaaagcct gtcatcactt tagtctacat cgacgtccat ctttaaagtg cattctgaaa
    26101 tattatattc agaaaccttt aaagtatgtc gtgtctatga ccattgtgac aacaggcttt
    26161 tttttattat catatacctc actattgaaa aggttatata ctcttttcaa tggtgaggat
    26221 tgttactgtg aatgggtatg accatcgcta tatacttata taaagtacac cttctttaca
    26281 aacaatgact tgatttaagg tgtgaaattt ttggcaatgg cttggcgaca tatgcattta
    26341 ttcaacactg ccaccagtct tacttcagac aaacttcaga ctaacctcga gttcgtatta
    26401 tgcccaccac atgtctgctg gcttgtcgta aatattaaca gatttcaaag tttgacaagc
    26461 tttttggaaa ccttcttcta ctgacataat aggatcttca tgttcaatac ttaatacata
    26521 atcatatcca ttaataataa gttgacttat gatatctgcc catacatatg gactatgtcc
    26581 ataaccaact gtacggaatg tccatgctct tgtcgcaacg ttaccatatg gttgcatatc
    26641 agttagacca tacatattta cattttcttg attaatatac gtatctttag catggaagtg
    26701 atgaattgca tttgcttggc ctaatatgcg aatcgcagca attgggtcaa taccttgcca
    26761 ccatagatga ctaggatcta agttagcacc gatatattca tttgtagcct cacgtaactt
    26821 caacattgta tatggtgtat gcactaaaaa tcctgcatgc aactctatgg caatttttac
    26881 atcttgctct tttgcaaact cagctaaatc ttgccaatat ggtatcaact tttcattcca
    26941 ctgataatca taaatttcag agtaggctgt tggccaaggt gtaacaggcc aattaggctt
    27001 tttagcggta tcatctgatc ctgcaatgcc agaaaatgta ttaacaactg gcacgtctaa
    27061 tagatttgct aaacggattg ttttacgtaa cgtttcatcg gcttctttcg cttctatcgg
    27121 atctggagaa attggattgt tatgacaact gaaaccactt atttgtaagc ctctgtctgt
    27181 gattgacttc ataaatgctt ggcgcttgtc ttcattttct aacaactcat ctaacttaca
    27241 aaatttatct cctgggttac cacctgttcc aacttcaatc atatccaatc cagattctga
    27301 gacataatct aacatatctt caaaattttt atcgtaaaat aatactgaaa atacacctat
    27361 tttcattgaa aagcactcct tctttttata ttgagaattt tgtatcatat atgataatca
    27421 ttaaagttgt atgctcttgt tatcaagatc gctacgataa atcgcttcta taagggcatt
    27481 tacattgcgt gcttcttccg gtttcaccac aatctcttct atccctaaac acgcattgac
    27541 aaagttacgc gcctgtctct caccagcaat gtcttcgtta tgctcaacat tagctttgct
    27601 ttcaaaaata gttccaaagc ggggctcata tatttcaaat ggaaataaat tgataccgcc
    27661 atcttctcct gataaactaa cgtgaacctt atcttctttg atatttgcag accacgaaca
    27721 ttcaaactgc atgcttgctc gattggcaaa tgtcatataa ctagtaacat gatcatcgac
    27781 atcaaattta gtatgatcaa atgttcccca atcattaatt tgattcggtt gtttgctcaa
    27841 ttgattatat gtttttccta gcacttcatg cggcaccata tctttaccta gtagccacaa
    27901 agataagtct aacaagtggc aaccataatc gattaaacta ccgccacctt gcaacgcttt
    27961 attggtaaaa acaccccagc caggcacttt acgcctacgc atcgcttgta cacgtgctac
    28021 taaaggttta ccaaccacac ctgattcaat tgctttttta gcagtaattg ccacatctgt
    28081 gtgacgataa tgatatgcga cagttaataa tttgtgattt ttattagccg cttcaatcat
    28141 gcgatcacac tcttccgtcg tcatcgccat tggcttttca cacaatacat ggacaccatg
    28201 gttcaatgct tctatagaaa gatcagcatg gaatttatta ggtgtacaaa tgaccaccgc
    28261 atcaacaagt ttaaacagct cgctaggtgt ctcaactgca tgaggtatat taaagcgctt
    28321 cgcaacatca atcatctgca ctgtattaat atcttgtact gcaactaatg agactgtgtc
    28381 tttgagtttc agcaatgctg gaatatgacg gtcttgtgca ataccaccaa cacctatcac
    28441 accaactttt aattttgtca tgatgtgcct ccttaccgta tgatgttatt caaagtaaat
    28501 tgctttgcct gattttgcag actgataaat cgcttcaaga atttttgtaa ctaccattgc
    28561 ttgttccggt ttcacaactg gttcagtatc atttacaact gcatcaatcc aagcttttgc
    28621 ttcttcttca gcttcatcca cttcattacc ttcataaaag tcgacgcctt tgttttccaa
    28681 ttcaacgtgt ttggtataaa gtgtacccat gtcttcacca tgaatacgta gaccatcttt
    28741 catatcagca cctgctttag ttcctgataa tgaacatttt gcctcatcca cttctaaaga
    28801 attaatcgcc caagcggatt ctaaaatgat cgtcgctcca ttcttcattt taataaatcc
    28861 aaacgcagaa tcttcaactg taaattcatc tggattccat gaaccccaag cgtttgccgc
    28921 atgatgctgt ttatttaatt tatggaatgt tgaacccatc actgattctg gttcataatt
    28981 atccatcatc cataacgtta aatctaaagc gtgtgtaccg atatcgatta atggtcctcc
    29041 accttgagct tcttcgtcta gaaagacacc ccatgttggt actgctcgac gacgaatggc
    29101 atgtgccttt ccgaagtaaa tgtctcctaa gtcgccacgt tgcgctgctt gatgtaaaaa
    29161 ttgactatct gctcggaaac gattttgata acctattgtt aattttttac ctgttgattt
    29221 agctgtatct atcatttttt gagcttctgc tgtcgtttta gccattggtt tttcacacat
    29281 cacatgttta ccagcatgca accctgctac agtaatttca caatgcgagt catttggcgt
    29341 acaaacatgg ataacatcaa tcgtgtcatc ttttaacaat gctttgtaat catcataaac
    29401 ctttgcattg tcagttccgt atgcttctgc cgcactcgct gctttcgaaa tgtctacgtc
    29461 acaaaatgcg atcatttcaa cattttcaac tttttgtaaa cttggcatgt gcttgccatt
    29521 cgcaatacca ccacacccaa tgattccaac tttaatcgtc atcataacgt cctcctttta
    29581 atctttatcg tcattagtgg ttctttatca attctgcccc actcctaatt tcaaactaac
    29641 cttttgtcgc acctgttgtt aaacctgata ctaaatagcg ttgcaagaac aagaatacga
    29701 ttgctatagg tactgcaatc ataattgccc ctgctgcaaa cactgtgaaa ttatttgcat
    29761 acttatcatt aataaagttg aacaatccaa ctgctaatgt gaatttttca ggacttctta
    29821 atagtatttt aggtaatata aagtccataa atggccccat aaaattgaac aaagcaacaa
    29881 ctgctaaaat cggcttagct aatggaagca taatttgtaa gaaaatacgc atatgccctg
    29941 caccatcaat tttggcagat tcatcaagtt cttttggaat cgtatcgaag taacctttca
    30001 ctaaaaaggc attcatcggt attgatccac caatatatac cagtgttagt ccaaataaag
    30061 aatctaataa tccaattgta tttagcaaaa tatagattgc gaccattgcc attaatacag
    30121 ggaacatttg taaaatcaaa aatgtaatca gcccgtattt acgaccaaca aagcgatatc
    30181 tagaaaaagc atatgctgtt aacgtgacaa atatcacact aaacagtgca tttgcagatg
    30241 ctacgataag cgtattttta taccaagtca ggtattgact actgtcatcg aatagtaaga
    30301 atgcataatt tttaaatgtt gcattgtctg gtatcatttt ggcaccatac aagttcgtac
    30361 ctggattaag ggaaatgcca aatgtccaca gtagtggata taaaatgatg acaaacatca
    30421 tcgctataaa actgtaaata ccgattgctt ttaatatgtt tttcttcttt gtcatcttat
    30481 aaacctccct catctttaaa cgtacttgta cgtctgaatt gaataaatgc gacaatagca
    30541 acaataaatc caataattaa tgacacaact gcacccatgt tgaagttgtt aaactcaaat
    30601 gtcagattat acacccaaga tatcaagata tctgtactac cagcattctg ccctgacact
    30661 ggtggaccgc ctttattaaa tagataaata agattaaaat tattgaaatt acctgcatat
    30721 tgcataatta acaatggcgc tgtggcgtaa atgacatgcg ggaatgtgat gtttctaaac
    30781 ttttgccaac tagacgcacc atccatatct gctgcttcgt accaatctga tgaaatactt
    30841 tgcagtactc cagtgaacag tgcaaagaca aatgggaatc caagccatac ttgaatgccg
    30901 attaatgcca cttttgccca aaacggatca cttaaccatg ctggtgctac acctaataaa
    30961 ggttgcaaaa tatcattatt tatcgcacca aattcatcat taaataacgc tacaaatatt
    31021 aaaattgtca caaatgatgg tacagcccaa ggtaggatta acacagtacg gataaatttc
    31081 ttacctttga cgacagggtg atttacaata attgccaaaa acagccctaa tgcaatttga
    31141 agtgtcgttg caacaagcgt ccatactaat gtccaagtaa taacactgaa aaatgtttta
    31201 cgccaaacgc caattgtgaa taacgtttta aagttatcta aaccaaccca ttctaatgtg
    31261 tgtctcggag gcgcgttgta taaattgtaa tttgtaaatg ctactccaaa cataaatatt
    31321 aatggaaata caactacaaa tatcaataag aatgtacctg gtgagattag taagtatggg
    31381 aacgtcttgt cccatgttgc caccatacgc gccttcggat cctttatttc ctcattgcga
    31441 ttaaatcgtt cagcattacg atatgcatct aaaatattaa tgatatatag catgattgcg
    31501 aaagcaacga gtaagataga aataatacct tgtgcaagta agacacgaga atcgtctaac
    31561 ttaggtactg tccctaatgt gaacaatccc caaaaaccaa tatttaaaaa gctataaaaa
    31621 acagaaataa aactgatgaa aaagataaaa aatatcgtcc ctttaatggg tcttttatta
    31681 taaaactgtc ccaaaccagg tataacagat aataatgccg ctaatttagg gttacgtttc
    31741 gtcataacta atctcctttc ttatcatttt gtgatggatg aagaatctta atattttgcg
    31801 ttatatcatt cgtcgcctca tctaacgctt gtttaggatt cttaccattt gaaataaata
    31861 tgcttgcatt gcccatcggt tcccaaactt gtcgcatttc aggaatatta ggcatcggtt
    31921 cagcatgacg tgcttgcttt tcaaacactt ttaaatttgg attagatgat ttcacgtcaa
    31981 cacgtccagt aatttcgctc atttcatctg tatatttttg taatgtatct ttactagtga
    32041 tatacagcat taaatcttta gcccaatact tatgtttact atattcagat aaataccaac
    32101 cacgtacacc tagaaatggt ttcataggtt tgccaccatc tgtaggtaat gttgttactc
    32161 ctaaatcttt accaaacgtt tcttgatatt cattaatgtt ccacggtcca gtgacaaatt
    32221 gtcctacttt tccttcttta aaaagaccaa tcatgacatc atgtgttgct gccttaggaa
    32281 gatacccttt gtcgtaccat ttttgtaatc gttcagcatt cttgacgaca tgttttgaat
    32341 ttagtcctag ctgatgaata tcatattcac tgccattttt cttgaaaata taatcatcat
    32401 tgccgaataa aaacggataa ttaaaataga aatttttagc atcaaataac ataccgtatt
    32461 gtttcttttt actatcagtt agtttggcag cattagcttc aacttcttct aacgtttgcg
    32521 gtgcattttt cactaatttt ttattataaa aaagtgcggt tgtttcaacg atagctggca
    32581 atgctagttg cttattgtca taattcatcg ctttaagtgc ttgcttattg aaacctttca
    32641 actcatcttt tgataatttg atttcagcag ctaagccttg tagataggca cttccagtat
    32701 tatcatgtgc taagaaaaag atatctggac cttttcctgc aggagcgtct agcgaaatat
    32761 tttctagttg atcattttga ccaatattta caagctttac tttgatgcca gtttttttag
    32821 tatattgatc cgtaattttt ttataaaacg ccatttgctt gtcgccatcc acccacatcg
    32881 taagttggtt aggcttgtct ttagaattat ctttattcaa tgctttatca atatcttctt
    32941 tcgaacgatt agggccacat gcagttacga ttaataacat taccacggct aacgtgatac
    33001 attttaaaat tttagacatg aatactcccc cttagacgat acgatttcct gttttttcat
    33061 caaaaaagtg acacttattc atatcaaatg ctagtgtaat cttgtcgttc gccatcactt
    33121 gagttcttga atctaattta gaaatcaatt ccattccttg gaatgtgcta tgtaccataa
    33181 tttctgaacc taacagttcg gatacaacta cttcagattc aaattgtgtc tctggagaag
    33241 tttgaataaa tattggttct tcatgaatgt cttcagctcg aatacctaaa ataatttcct
    33301 tgtctaagta gccagcagct tttaactttt caaatttttt attatgtaat ttaaaatggt
    33361 gtgttcctac cttcaatccg tccatttcaa ctgtagcatt caacatattc attgctggtg
    33421 agccgataaa ttgcgccaca aatatgcaat taggggcatc atatatttct ctaggtgtgc
    33481 cgacttgcat aatgtcgcca tctttcaaaa caacaattcg actagccatc gtcaatgctt
    33541 cagtttgatc atgtgtaaca taaattgtcg tagtattaag tcgcttatgt aatttcaata
    33601 tttctgtgcg catttgtact cgaagcttcg catcaagatt cgataatggt tcatccatta
    33661 aaaagacttt cgcatcccta acaatagctc tgcccaaagc aacacgttga cgctgtccgc
    33721 cagataacgc ttttggttta cgaccaagat actcagttaa tcctaatatt tcagctgctt
    33781 cattaacttt ttgttcaatc tcttttttat ttactttacg tagctttagc ccaaatgcca
    33841 tattttcaaa aacagtcata tgtggatata atgcatagtt ttgaaatacc atcgcaatat
    33901 ctctattctt tggttcaaca tcgttcatgc gttccccatc aatataaaaa tctccagatg
    33961 tgatagactc tagtccagca accattcgta atgttgttga tttaccacat cccgatggtc
    34021 caacaaatac aatgaattct ttgtcagtaa tatgtagatt aaaatctttc actacagtat
    34081 tgttgttatc atacgtcttt ttaatatgct ctaactttag ttctgccatg ttatgcctcc
    34141 catactttgt ttacagtttg attgtgcaat acacttcgtg aaaacgtttg cacaaatact
    34201 tacataaatc atatattcag tcattattaa tgtcaatcat aaattataaa cgtttacatt
    34261 actgtcagaa ttaaactttt atacagaaaa ttgatatagt gcacaactat tttctagtgt
    34321 gacaataatg ttttttatgt gcgatatttc actgccaatg ctaacaacct tgtcttacat
    34381 tttaagtaat atctaaatca aataagtatg acttagcatt acgattttca ttgaaaaaag
    34441 caaccattat gtaagtatca tactcacaca atgattgctt aattttttag actgcgggca
    34501 cccttctata actacaaatc atataggcta cgttccccat caggtaactg atcgataaac
    34561 ggttttggat cttctgcata tgcattacct acgccaccct tcattctttg aaaatgtaaa
    34621 tgtgcgcctg tcgtttgtat acctgtattg cctgaatatg caataatatc acctgctttg
    34681 actcgatcac ctactttgac attatatttg tctaagtgta gataccactg gtgatattct
    34741 ccattgtctt cggcaatctg taataccttg ccgcccaatt cattattaaa gatacgtgtt
    34801 actttaccat tcgtcggcgc tttaattggt gtaccttttg gcaatgcaaa atcaatgcca
    34861 taatgcttac catcaaatgg tgaatgttga tatttaccaa aagtctcagt aatccgactc
    34921 cctctaaaaa agtcttccca ttttgagtgt ccttgtcctt tatgattttg tgtcaattta
    34981 tcggtatatt ggttaaacca tttttgaatg tcatctatat aaataatcgt aaataatagt
    35041 gaaagaaaaa tgataattgt tgccaaaata cgttttggtc gctttgtcat agtggcatcc
    35101 atccttcata tattttgggt attaagtata tattctaaat caactatttc ataattaaat
    35161 gcactcatac tttagtagta ctgccaaata tagatgaaga aaatgatggt aatttgaata
    35221 ttttgattga tattaaattt tatatcgagt ataataatta tacttaagaa aataacagaa
    35281 acagaggaga attttcaaat ggcaaaagta ttatatatta cagcacaccc ttttaatgaa
    35341 ctggtatcaa attctatggc ggctggtaaa gcatttatcg aaacatatca acaacaacat
    35401 cctgatgatg aagtgaaaca tattgattta tttgaaactt atattccagt tattgacaaa
    35461 gatgtattaa ctggttgggg aaaaatgagt aatggcgaaa cgttaactga tgatgaacaa
    35521 atgaaagttt caagattgag tgacatttta gaagaatttt taagtgcaga taaatatgta
    35581 tttgtaacgc ctatgtggaa cctttctttc ccacctgtag ttaaagcata tattgatgct
    35641 atttcaattg caggtaaaac ttttaaatat tctgctgaag gaccacaagg tttactaact
    35701 gacaaaaaag tattacacat tcaatcacgt ggtggatatt atactgaagg acctgctgct
    35761 gattttgaaa tgggtgaccg ttatttaaga acaattatga cattcttagg tgtgccatca
    35821 tatgaaacaa tcattattga aggacacaat gctgaacctc ataagactga agaaattaaa
    35881 gcaactagta ttaataatgc tgaaaaatta gcaacaactt tttaaatttt tagttacact
    35941 gtgtgacgtc tcaaacgtca tgcagttttt tattgtatct cttttccgct ttttataaat
    36001 atctattatt ttcataaatt taaaatttga tattcacttt aatactgatg taaatatttt
    36061 tcaaaatata ttattatagt gttaatgaag tattttgaga aacggtggga atgtatgaaa
    36121 gcacataaaa ttttctggtt aaacttagca gcaattatta ttatttcaat tgtagtcagt
    36181 ggcgatatgt ttttggcaat gaagtgggaa caaattcatt tgaaagatgg tcttaaaaaa
    36241 gtactaagta cgtatcctat taaaaactta gaaacgcttt atgaaattga tggtcacgat
    36301 aatccccatt atgaaaataa tgatcaagac acatggtata tagaatcctc ttattcagtt
    36361 gttggatcag atgagctttt aaaagaagac cgtatgttgt taaaggtaga taagaacaca
    36421 cataaaataa ctggtgaata tgacacaact acaaacgata aaaaaaatgc tactgactcg
    36481 acatacaaaa gttatccagt aaaagtagtt aataataaaa tcgttttcac aaaagacgta
    36541 aaagatcccg cgctgaaaca aaaaatcgaa aacaatcaat ttttgattca aagtggtgat
    36601 ttaacaagca ttttaaatag caatgatttg aaagtcacac atgatcctac cactgattat
    36661 tataatttat ctggtaagtt gtcgaacgat aatccaaacg ttaaacaatt aaaacgtaga
    36721 tataatattc ctaaaaacgc atcaacaaag gtggaattaa agggaatgag tgatttaaaa
    36781 ggcaataatc atcaagatca gaaactttat ttttattttt caagtcctgg aaaagaccaa
    36841 atcatttata aagaaagcct tacttataat aaaataagtg aacattaata cttatgctgt
    36901 aattatagaa acatccaaat catctattaa aatcctatat tataaaaaca cctcacataa
    36961 ctcgttcaac tgtaccaaac cacattacat tagattttag gctaactatt gtgatgtaca
    37021 tcaaaaacga atttgtgagg cgttgtatat tttacaaagg tgactagcgt ttcgtatagc
    37081 atttccaaca ttactacact caagcgtcac gctaaagttc gaaatcgaat cctttcattc
    37141 aacaaaagct catatccact acaaacttca tatcaagcgt ataaactatc ttgtgatact
    37201 atctcgatca tatctatagt atgcatttgt gttccgtttc actgaagtat atgtatcatc
    37261 agttaagtat aaaccgtcat ccttcaatgt tacttgataa gcatatttcc gtgctaacca
    37321 ggcaatatct atataatttt ctcctgcgtt ttcataactt cttaaatctt caatatgtgc
    37381 actaacttca gggaaaatga ttctaacaac actttcatca acccaatatt tgtcatgcat
    37441 ccatcgcact tgatctgcca ataaaggtaa ctgcacatca ttgaaatata gacgaaagcc
    37501 gtcactatca tacatttgcc gatatggtaa tggctgtttt ctaatcacta acacctcgcc
    37561 acccattacg gtgccttctc tagtatcatc acttccaccc gaagcttcat acgttgttgg
    37621 gtcaacctgt agtccatgta catctccaat ataagcatct ggtttatgtt ccattgcatg
    37681 tccatgtgca atcaatgcta atattgtaga ttgtgaaaat tgaggctccc attcaatgcg
    37741 attaggatgg ctactataaa ttctaggttc atctatagcc tgctgaatat ccatgccaaa
    37801 cactaataca ttgattaatg tttgcgcaac actagcaatg atacttatgg caccaggtgc
    37861 acctactgtt aatattggct tcccgtgata catcacaatc gttggagcca tgttacttag
    37921 tggtcgttta tatggtgcaa tttcgttaat accaccatct actacatcaa agccatccat
    37981 tgtcgtattc aataacacac cgtagcctgg aatcgtgata cctgaaccat aaatcatacc
    38041 aattgatgtc gtaaatgaag caatattacc ttccttatca atcacacaaa aatgagttgt
    38101 ttcggtatga ttttcttcaa catctgtatg actaatgcaa tcagacacaa caccatgctc
    38161 tatatcaata tttgctttat tgctatcaat gagcgtactg cgtgctttta aataatcatc
    38221 atcaattaat gactgtacag gcacctcatg aaaattatca tccgccaagt attgcgcacg
    38281 atcactatat gctaaatgca tcgcttgtat caaatgatgc aagtaatcaa cagatcttgg
    38341 acccatagat ggtaaatcga catgttctaa taacttcaat atttgaatta ccgtgatacc
    38401 gccagaacta gatggtccca ttgaataaat gtcatagtct ttaaatgttg cactgattgg
    38461 cgctttaatc tgaatgtcat atttggctag atcctctaaa gtgattgtcc caccacatgc
    38521 tttgacaaca ttgactaatt gtttcgcaat gtcaccttta taaaatgcat taaacccttg
    38581 ttctcttaat atttgaaatg tcttacctaa ttcgggttgt acaatccaat caccttcacg
    38641 ccaatattga ttttcatgcg taaatacttg tgccgtttca tgatactttg tcaatcgtgc
    38701 gtgttgctgg cgcgaatatt tttcagtagc ccaattggct gcatgacctt caatggctag
    38761 ttcaattgca ggattaatta aatcttccaa tgacaattta gcataacgct tgtgaatata
    38821 atcaaacagc tttggaattg ctggcacagc gacagtttta ccatgtgtag tcatatcaaa
    38881 aaatgattta tattcgcctg aatcatctag ataaaattgt ttgtctacat gttcaggtgc
    38941 tgtctcacgt gcatcaaacg cagttatact gccagtactt tgctcataat atagcaaata
    39001 cccgccacca ccaatacctg atgcaaatgg ttctaacaca ttcaatgcca gttgaattgc
    39061 aatcactgca tccatggcgt tgccaccttg atctaataca tccttaccaa ttttagccgc
    39121 aagaggatgt gatacggaaa ttaacccttc tttagatgtt tttgtctgtt tgtcatttaa
    39181 gttaatgacc atactatatc ctcctacttt ctgttaaata tttaaaacat tattgattaa
    39241 tggctttttc tactttttct aaatcttgac gttgctcgtt accagtatcg acaagtggtg
    39301 taatcggtga tgcaatttta aatttatcgc cacgataaaa cttaataaat tgatcctgat
    39361 ctatcgcatt aactactgct tgtctcaagt ttggatgcgt cttaaatata ccttttttaa
    39421 tatttagcat taaaaagact gacttgcgtc catttttgcg aataatgctt aaatttttat
    39481 ccgacttaat taaatcaaaa tgtttttgat tcacatctgc caacatatca attgaatgat
    39541 ttctaagttc tgacaatgca ttattcgggt caccattaaa cttcaatgta atatttttaa
    39601 ttttagctgg tccataacta cctttttctg tttcgttgaa tcctggatta cgttgaaacg
    39661 ttgcttgata tgcatttttc tgtgtcataa tgtatgcgcc acttgcatac agcgcatttt
    39721 tcccatctga atttgcagga attgtactgc tatccccata tccttttgga tattcttgat
    39781 ttacttgatt aacaaatttt ttagataaaa tgcctgccga agagtgtgtt aagtaattta
    39841 cctctcgagg catcgattga tctgtcgtaa ttttaacaat ttgataaata ccgtctttat
    39901 tatttacttt ttgaccatct gtcgttaacg atttgacgtt ataagcttta atcaacttat
    39961 catagattga tttatcgtcc ttgtctttct ctttacgcaa ctgatcgatg tcctcatctt
    40021 ttaatatctt gatgtcattt atatgtttgt gcatattgta agtattattg ttaggcacag
    40081 actttttatc acgtgctcta tctaaagaaa acttaacatc ttcagccgat acacgctctc
    40141 cagtattacg tgcttgtcca ttgaccactt tcgcaaaata atcatcatct cttaacaaga
    40201 aataaaatgc tttattgtcc ttattcacag cataatcatg acttaacgaa cctttcgttg
    40261 ttaaatgatc attttcatct aataataata accttgtgta catattcata ttaattgaat
    40321 atactgacgg cgcaattgaa cgtattggat ccaatgtagg aatttcacca tcttgttgtg
    40381 tcatcacaag tggccgcgta tctcgttctc tactattgtt gtaatcaaat tgttgccata
    40441 ttaatgcacg tgaatttggc aatccaacac tatttttatc taacacttta ttgtcatata
    40501 ctaaattctt ttttgatcca tataaaggcg ccatataccc tttatcaaat acaacttcat
    40561 cttcaatttg cttatatgtt tgtttaacat ctgcttcatt ttgagtagaa gctttattta
    40621 acaactggtc tacatgttta tctttcaata aactatttga tcctgtagaa ctaaataatg
    40681 ccgtcatagc atagttcggg tcaccaaaca ctgtcatcca gtcatcaatt tggatatcat
    40741 aattgccggc ttgacgttgt gtacgatagc taccataatc tggttggata ttcatcttca
    40801 cgttaaatcc tgcattttcc aattgatctt taacgatatt catatcattt tcataacttg
    40861 cttgtcctag gaaatgtatt gttggtcgct cgcctttcac ttcaactttc gatgactttt
    40921 gagccacttc tgatttcgta gggacaccac aaccacttaa taccaacgct aaaactataa
    40981 ttgcgatact aatgattttc ttcacatcta tccctacctt tttaatgaat tcttggatct
    41041 agtgcatcac gcactgcatc acctataaaa ttaaatgcta aaacgacgaa cataatacaa
    41101 acaccaggta caatagctaa attactgtgc gtttccaagt agttactacc ggtacgtaaa
    41161 atgttgcccc attcagctac atcaggtgca acaccaagtc ctaggaaact taaactactt
    41221 gttgttaata caaccacacc tatatttaat gaaaaacgta caatcatagg cgcaatcgca
    41281 ttcggtaaaa tataacgcca tatgatattc caagtgtttt caccagtgat acgtgctgca
    41341 tctacatatt ccatgcgttt aatttctaaa acactggcac gcattgtccg tgcaaatgat
    41401 ggtatattac cgatacttaa agcaataatt aaatttggaa tacttgctcc aaatgatgca
    41461 ataattgcca ccgctaacaa taatgatgga attgcaaaca ctacatctaa aattcgcatt
    41521 attaaattat caatatgatt aaaataacct gcgatagtgc ctagtaacac accaaaaata
    41581 actgcaataa ctactgaaat aattgaaatt gaaaatgtca gcttcgttcc tacaactacg
    41641 cgtgtaaata agtctctacc gaaatcatca gtaccaaacg gataggctag actcggtcca
    41701 tgtaacagtg cattgaactg atttttagta gccaatgtcg tatcaaatgt aaattgtgac
    41761 acaattgata atgtcagcat gtagactaaa ataagtaacc cgataatcgc aatacgatgt
    41821 ctagtagttt ttcgtataaa cgattcccac ccgttataac tatgtatttg cgatgtacgt
    41881 tggtaacgtc taatacttac aaacattaat aatgtaaata cgttgcctgt taatgtcatc
    41941 aacaataaca acacttcgac gatacgtcgc cataggtcat gatgcttcca tgtttgttcc
    42001 gttgttaaaa taataattaa aatgatggtt aaaacgatta gcaatgtttc agcaatatag
    42061 aacgtatcgg ccacataacc tttaaaaaga tttaatgcac tcgttaatat aactaaaata
    42121 taagttgcta tggcgtaact tgcgaataat tttaaggaag ctatctttga attaagttgt
    42181 gccatatgcc tcacttcctt tcgttgattt cactacgtaa ttttggatcg attaaagcat
    42241 aaaatatatc aataattaag tttgctaaag atattacaat tgatatatat acgaccccac
    42301 ccatgactgc tggaatatca ggtattagtt gtttttggac gatataacgc ccgataccat
    42361 taatgttaaa tacttgttcc gtcactgctg aaccgcctag taactctgcc actagaagac
    42421 caactaacgt tacaattgga ataatggcat ttttcaaaat atgtttaata acaacttgtg
    42481 tcgtcgataa tccttttgca taagcagtta aaacataatc gctgcgcatt acttcaagta
    42541 cagaagacct tgtcatacgc gtgatagaag cagcaatact tgttccaatg acaagtacag
    42601 gtaaaatcaa cgatattgga tgttctggca tataagatgg tggcaaaata tccaatttca
    42661 atgagaacgc taaaatgaat aatagccctt gccagaaact tggaatagat aaaccaatta
    42721 atgcaattat cattaacgtg atatcaagcc aactatttcg cttcatcgca ctgataatac
    42781 caattggtat tgcaataatt aatgccacca ttagcgctaa tactgcgaca attattgtaa
    42841 ttggaattct ttcgccaact gctttagtca caacctcatt ccctttgtaa gtcgtaccta
    42901 agtcaaaggt aaaaacaccc ttgatggtat cccacaattg aataaaataa ggttcgttaa
    42961 gatgatgtaa tacattgaat tgatgtatct gtgcctttgt tgcattttgt cccagtatgc
    43021 tataagccgc atcaagcggt gaaaaataca gaatggtaaa cacactgaca ataacaccaa
    43081 tgatgacaat cacagccatg acaattcgtt caaaaatata tctaactaat ggctgtaaat
    43141 aaaaagtcaa taagatgaac atcggcaagg ccaatatcac tttgatcatg atgaacttat
    43201 gaaataatac attttcaaag tatgttgaaa aatgtgcttg ttcaatattc tttgaactcg
    43261 tattagaact ttgtgccttg aatattttta atgcttcttt atgtatttgt gtggatgact
    43321 tttgctgcga taaatattta tatttttgat gtaacgcctg ttcaatttct gaaatttcag
    43381 aattattagc gtaaaaattt ttcctcttag cagaaaagaa aaactttatc actgcatata
    43441 aaaatattgg caagcttaat accgataata caaactt
//
LOCUS       DANJFZ010000007        43450 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.007, whole genome shotgun sequence.
ACCESSION   DANJFZ010000007 DANJFZ010000000
VERSION     DANJFZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.007"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            58..438
                     /locus_tag="PWN45_000300"
     CDS             58..438
                     /locus_tag="PWN45_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0622099.1"
                     /translation="MLKDLQNEYEISLEQSNVLGMLNKEPLTISEITQRQGVNKAAVS
                     RRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDKGRAYLKERNAIMTDIAQDITNDLNS
                     EDIENVRQVLEVINHRIKTYSNHK"
     gene            678..2060
                     /locus_tag="PWN45_000301"
     CDS             678..2060
                     /locus_tag="PWN45_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622100.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2091..2219
                     /locus_tag="PWN45_000302"
     CDS             2091..2219
                     /locus_tag="PWN45_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622101.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2329..3537
                     /locus_tag="PWN45_000303"
     CDS             2329..3537
                     /locus_tag="PWN45_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDL0622102.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(3767..4318)
                     /locus_tag="PWN45_000304"
     CDS             complement(3767..4318)
                     /locus_tag="PWN45_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0622103.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            4439..5086
                     /locus_tag="PWN45_000305"
     CDS             4439..5086
                     /locus_tag="PWN45_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL0622104.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            5099..7030
                     /locus_tag="PWN45_000306"
     CDS             5099..7030
                     /locus_tag="PWN45_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDL0622105.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(7276..7914)
                     /locus_tag="PWN45_000307"
     CDS             complement(7276..7914)
                     /locus_tag="PWN45_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622106.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            8122..9042
                     /locus_tag="PWN45_000308"
     CDS             8122..9042
                     /locus_tag="PWN45_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0622107.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(9150..9569)
                     /locus_tag="PWN45_000309"
     CDS             complement(9150..9569)
                     /locus_tag="PWN45_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622108.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(9566..10057)
                     /locus_tag="PWN45_000310"
     CDS             complement(9566..10057)
                     /locus_tag="PWN45_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDL0622109.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            10154..11101
                     /gene="corA"
                     /locus_tag="PWN45_000311"
     CDS             10154..11101
                     /gene="corA"
                     /locus_tag="PWN45_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL0622110.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            11132..12181
                     /locus_tag="PWN45_000312"
     CDS             11132..12181
                     /locus_tag="PWN45_000312"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL0622111.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(12229..13437)
                     /gene="gltS"
                     /locus_tag="PWN45_000313"
     CDS             complement(12229..13437)
                     /gene="gltS"
                     /locus_tag="PWN45_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDL0622112.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(13774..14382)
                     /locus_tag="PWN45_000314"
     CDS             complement(13774..14382)
                     /locus_tag="PWN45_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL0622113.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            14781..15428
                     /locus_tag="PWN45_000315"
     CDS             14781..15428
                     /locus_tag="PWN45_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL0622114.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            15659..16558
                     /locus_tag="PWN45_000316"
     CDS             15659..16558
                     /locus_tag="PWN45_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622115.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            16551..17783
                     /locus_tag="PWN45_000317"
     CDS             16551..17783
                     /locus_tag="PWN45_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622116.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            17767..17952
                     /locus_tag="PWN45_000318"
     CDS             17767..17952
                     /locus_tag="PWN45_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622117.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            18001..18327
                     /locus_tag="PWN45_000319"
     CDS             18001..18327
                     /locus_tag="PWN45_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDL0622118.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            18357..19376
                     /locus_tag="PWN45_000320"
     CDS             18357..19376
                     /locus_tag="PWN45_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0622119.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(19612..20265)
                     /locus_tag="PWN45_000321"
     CDS             complement(19612..20265)
                     /locus_tag="PWN45_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL0622120.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            20439..21125
                     /locus_tag="PWN45_000322"
     CDS             20439..21125
                     /locus_tag="PWN45_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDL0622121.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(21191..22450)
                     /gene="sdpC"
                     /locus_tag="PWN45_000323"
     CDS             complement(21191..22450)
                     /gene="sdpC"
                     /locus_tag="PWN45_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDL0622122.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            22740..23675
                     /locus_tag="PWN45_000324"
     CDS             22740..23675
                     /locus_tag="PWN45_000324"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDL0622123.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(23931..24350)
                     /locus_tag="PWN45_000325"
     CDS             complement(23931..24350)
                     /locus_tag="PWN45_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDL0622124.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(24472..25356)
                     /locus_tag="PWN45_000326"
     CDS             complement(24472..25356)
                     /locus_tag="PWN45_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0622125.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            25516..27177
                     /gene="hutU"
                     /locus_tag="PWN45_000327"
     CDS             25516..27177
                     /gene="hutU"
                     /locus_tag="PWN45_000327"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDL0622126.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            27177..28415
                     /locus_tag="PWN45_000328"
     CDS             27177..28415
                     /locus_tag="PWN45_000328"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDL0622127.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            28692..29813
                     /locus_tag="PWN45_000329"
     CDS             28692..29813
                     /locus_tag="PWN45_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDL0622128.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            29924..30805
                     /locus_tag="PWN45_000330"
     CDS             29924..30805
                     /locus_tag="PWN45_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0622129.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            31292..31861
                     /locus_tag="PWN45_000331"
     CDS             31292..31861
                     /locus_tag="PWN45_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622130.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      31914..32009
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            32086..33399
                     /locus_tag="PWN45_000332"
     CDS             32086..33399
                     /locus_tag="PWN45_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL0622131.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            33566..34072
                     /locus_tag="PWN45_000333"
     CDS             33566..34072
                     /locus_tag="PWN45_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDL0622132.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(34130..34894)
                     /locus_tag="PWN45_000334"
     CDS             complement(34130..34894)
                     /locus_tag="PWN45_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0622133.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            35224..36828
                     /locus_tag="PWN45_000335"
     CDS             35224..36828
                     /locus_tag="PWN45_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0622134.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(36894..37424)
                     /locus_tag="PWN45_000336"
     CDS             complement(36894..37424)
                     /locus_tag="PWN45_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622135.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            37692..38609
                     /locus_tag="PWN45_000337"
     CDS             37692..38609
                     /locus_tag="PWN45_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDL0622136.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            38684..39319
                     /locus_tag="PWN45_000338"
     CDS             38684..39319
                     /locus_tag="PWN45_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0622137.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            39330..39521
                     /locus_tag="PWN45_000339"
     CDS             39330..39521
                     /locus_tag="PWN45_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622138.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(39617..39802)
                     /locus_tag="PWN45_000340"
     CDS             complement(39617..39802)
                     /locus_tag="PWN45_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622139.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(39799..40155)
                     /locus_tag="PWN45_000341"
     CDS             complement(39799..40155)
                     /locus_tag="PWN45_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622140.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            40435..41814
                     /locus_tag="PWN45_000342"
     CDS             40435..41814
                     /locus_tag="PWN45_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0622141.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(42201..43073)
                     /locus_tag="PWN45_000343"
     CDS             complement(42201..43073)
                     /locus_tag="PWN45_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0622142.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT        14834 a         6070 c         8097 g        14449 t
ORIGIN      
        1 gaccatattc aatttttaga gcagtttata aataacgtta acgcattaac tgcaaaaatg
       61 ttgaaagatt tacaaaatga atatgaaatt tcattagagc agtctaacgt attaggtatg
      121 ttaaataaag aacctttgac aattagtgaa atcacgcaaa gacaaggtgt aaataaggcc
      181 gcagtaagcc gacgaattaa aaagttaatc gatgctaaat tagttaagtt agataaacca
      241 aatttaaata ttgatcaacg tttgaaattc ataaccttaa ctgacaaagg tagagcatat
      301 ttgaaagaac gtaatgcgat tatgacagat attgcgcaag atattactaa tgatttaaat
      361 tctgaagata ttgaaaatgt aaggcaagta ttagaagtca tcaatcatcg cattaaaaca
      421 tatagcaatc ataaataggt ttttgaaggt gttcaaatat aagactaaat atgtatgtat
      481 tgaacacctt ctttttagtt ataattgaga gagtactttc ataaataatg taataaggta
      541 caattctaaa gtaaagttaa tcatttcttg aattgtattc ttgggataat atttgtaaaa
      601 taataatggt aaaataatga aataattttt gtgatataca tataacaatt gaagaggaac
      661 agaattgagg tgaaagtatg aaatcttgcc cgaagtgcgg acaacaagca caagatgatg
      721 tacagatatg tacacaatgt ggacataaat ttgatagtcg tcaagcatta tatagaaagt
      781 caaccgatga agatatacaa actaataaca tcaagatgag aaaaatggtg ccttgggcaa
      841 ttggtttttt cattttaatc ttaattatta tattgttctt tttactaaga aacttcaatt
      901 cacctgaagc gcaaactaaa atattagtga atgcgattga gaataatgat aaacaaaaag
      961 tagcaacatt attaagtact aaagataata aagtagattc tgaagaagca aaagtataca
     1021 ttaactatat caaagatgaa gttgggctta agcaatttgt cagcgacctt aaaaatacgg
     1081 tacataaatt gaataagagt aagaccagcg tagcttctta tattcaaacc agatctggtc
     1141 aaaatatatt acgtgtaagt aaaaatggca cacgttatat ctttttcgat aatatgagct
     1201 ttactgcacc taccaagcaa ccaattgtta aaccgaaaga aaaaacaaaa tatgagttta
     1261 aatctggtgg taagaaaaag atggttatag ctgaagcaaa taaagtgacg ccaataggta
     1321 attttatacc ggggacatat agaattccag ctatgaaatc aactgagaac ggtgattttg
     1381 caggccattt aaaatttgat tttagacaaa gtaattctga aacggtagat gttactgaag
     1441 attttgaaga agcaaatata tctgtaactt taaaaggcga tacaaaatta aatgatagtt
     1501 ctaaaaaagt aactataaat gaccatgaaa tggcattttc aagttccaaa acgtatggtc
     1561 catatccaca aaataaagat attaccattt cagcttcagg taaagcgaaa gataaaacat
     1621 ttacaacaca gacgaagacg attaaagcta gcgatttaaa atacaataca gagataactt
     1681 tgaattttga cagtgaagat atcgaagact atgttgaaaa gaaagaaaaa gaagaaaaca
     1741 gcttgaagaa caaattgata gaattctttg ctggatattc tttagcgaat aatgctgcgt
     1801 ttaatcagtc ggattttgat tttgtatcat catatataaa aaaaggatca tctttttatg
     1861 atgatgtaaa gaagcgtgta tctaaaggaa gtttaatgat gattagttca ccacaaatta
     1921 tagatgctga aaaacatggt gataagatta ccgcaaccgt aagattaata aatgaaaacg
     1981 gtaagcaagt agataaagaa tatgagcttg aacaaggctc gcaagaccgc ttgcaattaa
     2041 tcaagacatc agaaaaatag agtataaaac ttaaataaga tgcatgtgtc atggaataca
     2101 aacaacatcc tttcaaagta tttacaatta gtgtgaatta ctttgaaggg attgtttttt
     2161 gtggaaaaat ttatgaagtg ctgaggttta tatcatttta taccttttat aagttgtaat
     2221 gatgttcatg aaacttgtga ctaatgctaa tatataaaag agcatattta aatttagttt
     2281 ctgttcgtga tagttataaa ctaatcatta tataaaggag atagtcatat gaatgattta
     2341 acgcatttta acaagcgttc accgatattt attattattt taggatcttt gacggccatt
     2401 ggagcattat cgatagatat gtttttgcct ggattaccag atattagaca tgattttcaa
     2461 acaacgacgt ctaacgcaca attaacatta tcgatgttta tgattggttt agcgtttggt
     2521 aatttgtttg ctggtccaat ttcagattca actgggcgca gaaagccgct tattatagct
     2581 atgattattt ttacattagc aagtttaggt attgtttttg tacataatat ttggctgatg
     2641 gttgcattaa gatttcttca aggagtgaca ggtggcgcag cagcagttat ttcaagagct
     2701 atagcaagtg atatgtatag tggtaatgaa ctaactaagt ttatggcatt attgatgctt
     2761 gttaatggta ttgcaccagt tgttgcgcca acaatagggg gcataatttt aaattattct
     2821 gtctggcgta tggtctttgt cattctgact atctttggct tcgtcatggt cataggttct
     2881 ttattaaaag tacctgaatc attaacagtg acaaatcgtg agtcaagtag tggattaaag
     2941 acaatgttta aaaacttcaa aatattattg aagacgccac gttttgtatt accaatgctc
     3001 attcaaggta tgacatttgt aatattattt acttatattt ctgcgtctcc gtttattatt
     3061 caaaaaattt atggcatgac tgcaatacaa tttagttgga tgtttgcagg aattggtatt
     3121 acgctcatta tatcaagtca acttactggg taccttgttg attttataga ctcacaaaaa
     3181 ttgatgagag gcatgacgat gattcagatt atcggtgtaa ttttagtgac gattgtattg
     3241 cttaatcatt ggaacttttg gattttagcg ataggattta tcattttaat agcaccagtg
     3301 acaggtgttg cgacacttgg ttttacaata gctatggatg aaagtagtag tggtagaggg
     3361 agttcatcaa gtttattagg tttagtacag tttttatttg gtggtgttgc atcgccatta
     3421 gtgggggtaa aaggtgaaga taatcctata ccttacataa ttattattat agctacagca
     3481 gttatattaa ttattttaca gatttataat atgaaggtat ttaaaacaaa tcgatgataa
     3541 acatagtttc ttctattata ttgaattgaa aaattaaaaa acaaaacttg agctattcat
     3601 agacctatga atttagcatc aaaaataaaa atgccgagat tattatgtgg aaatctcggc
     3661 atttttatat tgatagcacc gttacatatg tactattatc tatccatttg gtttaacttt
     3721 atctagttgt tttatagcat ttctttatta gaatgaaacc gtattattag ttttgaagtt
     3781 taatcgtatt agccaataaa atgtcagcaa cttcttcagg tgattcatta caaccattcg
     3841 aaagccaatt aataaacacc ccagcttgac cgcctatgat atatgtaaca taaagctgtt
     3901 tattggttac tgtagtacta tagtcattca taatttgttg atagtaatct ctcgctaaat
     3961 ttatatagtc tatgaataat gttttcattg gataggtaac taaaatgtct ttgaaaaact
     4021 gctcattgtg cttgatatac ttagcaataa taatgaagaa tttataaagt ttaagcttat
     4081 cctgtttaat attttcgaaa ttatgatgta gcgcgcttag aagaaattgg aatttttcct
     4141 tatgctttga gttgatggta tccaataaat caaatttatc tttgtaataa tcataaaaag
     4201 tagaacgatt aatattacaa tacgcgcaaa tcattttagt ggatatttct tgaaaaggat
     4261 attcagccat caaatcaaca agtgcgtttt gaatttctat tttggcttgt cgtttcatat
     4321 tgttacacct ttgtgaattt taaccaacac tcttataaaa attgttggtg tttttttagt
     4381 tatcatgtct ctataataaa taaagtgaat tttaaaagca aataggagga aatgtttcat
     4441 gaaaaagatg atattaatta atgtcattac ggttgttgta ctgttagcaa ttgggattgc
     4501 tggcttttat ttttggaata aaacaacaag ctatgtaaca actgataatg caaaagtaaa
     4561 tggcgatcaa attaaaatag caagtcctgc atctggacaa attaaatcac ttaatgttaa
     4621 acaaggtgac aaactcgata aaggtgacaa agtagcaatt gttactgtac aaggtcaaga
     4681 tggcgaaacg aaagatatgg atcttaaaat gcctcaaaaa ggtacaattg ccaaattaga
     4741 tggtatggaa ggttcaatgg tgcaagctgg taacccaatc gcttatgcat acaatttaga
     4801 tgatttatat gtaacagcaa atattgacga aaaagatatt aaagatgttg aagtaggtaa
     4861 agatgttgat gtgactattg acggtcaaaa agcttctatt aaaggtaaag ttgatagtat
     4921 tggaaaagca actgctgcaa gcttttcatt aatgccatca tcaaatagtg atggtaacta
     4981 cactaaagtt tctcaagtaa tacctgttaa aatcactcta gaatcagaac cgtctaaaca
     5041 agttgttcct ggaatgaacg ctgaagtaaa aatccataaa aattaagggg gtcattaaat
     5101 gactacgacc ttcattatta gctacattat tttagcgctc attattgttg gggttatcaa
     5161 tttattttta ataagatcaa gaaaaaaagg caaacgccaa caaaaggaac aacaatttac
     5221 gacacgtcaa tcaaatcaat ctaaatttaa agctagtgat ttagacaaaa caactgatca
     5281 atcgacacaa cgtatgacgc atgaagagtt gcgtgttgac aatcaagatg atcatagcca
     5341 agttagtcta aatggttaca caaaggggtc tgagaaagat caagaagcat tcactaataa
     5401 taaggatgag gaagcagttg ctgctaaaaa tcctgaatca gaagaatata aagtgaatga
     5461 aaaaataaaa aaagaacata aaaactttat ttttggtgaa ggtgtttcac gcggtaaaat
     5521 attagcggca ttattattcg gtatgtttat tgcgatttta aaccaaacat tattaaatgt
     5581 tgctttacca aaaattaata cggaatttaa tatttctgcg tcaacagggc aatggttgat
     5641 gacgggattc atgttagtaa acggtatttt aataccaatt acggcgtatc tatttaataa
     5701 atattcatat cgaaaattat ttttagttgc gttagtactc tttacaattg gttcattaat
     5761 ctgtgcgatt tcaatgaatt tcccaatcat gatggttgga cgtgtattac aagccattgg
     5821 tgcaggtgta ctaatgccat taggttcaat tgttattatt acaatttatc cacctgaaaa
     5881 acgtggtgct gccatgggta caatgggtat cgcaatgatt ttagcacctg caattggtcc
     5941 aacattatca ggttatattg tacaaaatta ccattggaat gtgatgtttt acggtatgtt
     6001 cattatcggg attatcgcta ttttaattgg ttttgtatgg tttaagttat atcaatatac
     6061 aacaaaccct aaagctgata tcccaggtat catttttagt acgattggtt ttggtgcttt
     6121 gttatatggt ttctcagaag ctggcaacaa aggttggggt tcagtagaga tagaaacaat
     6181 gtttgcgatt ggtattatct ttattattct attcgttatt agagaattaa gaatgaaatc
     6241 accaatgttg aatttagaag tattgaaatt cccaacattt acattaacaa caattattaa
     6301 tatggttgta atgttaagtt tatatggtgg tatgatttta ttaccgattt atttacaaaa
     6361 tttacgcgga ttctcagcat tagattccgg attgttatta ttacctggtt ctctaattat
     6421 gggtctacta ggaccatttg caggtaaatt gttagatact attggtttaa aaccattagc
     6481 tatctttggt attgctgtaa tgacttatgc aacatgggaa ttaactaaat taaatatgga
     6541 tacaccatat atgacaatca tgggtatcta tgtacttcgt tcatttggta tggcatttat
     6601 aatgatgcca atggtaactg cagctattaa tgcgttaccg ggacgacttg cctctcatgg
     6661 taatgctttc ttaaatacga tgcgtcaatt agcaggctct ataggtacag caatcttagt
     6721 tactgtaatg acaacacaaa ctacacaaca cttatcagct tttggggaag agttagataa
     6781 aacgaatcct gttgtacaag atcatatgca tgaattagca tcacaatatg gcggacaaga
     6841 aggcgcaatg aaagtgttac tacaatttgt aaataaacta gcaacggttg aaggtattaa
     6901 tgatgcattt atagttgcaa cgatatttag catcatcgcc ttaattttat gtttattttt
     6961 acaaagtaat aaaaaagcaa aagctacagc tcaaaagtta gatgcagata atagtatcaa
     7021 tcatgaataa ataaaataaa ttaattgaag tgtgactaat caaaaattat gttgtgggga
     7081 catgattttt aaagtatcgg tgccaaatat ggttatcgat acttttttta tttgttgatt
     7141 tatagaatgt tagaggaatt atattaaaat ttggcattga cgtagtaggt cattaataaa
     7201 gaaaaagcag gaagtgggtc aacgaaatga attttgtgaa aataacattt ctgtcccaat
     7261 ccctactata taacattatt ttaaacgagg catgcgatta cggaagaata agcttataac
     7321 aagtaaaccg atgctacagc caagtaaaat gatgccgtta tgaatagcgt cagcttctgt
     7381 aatcacttga tctggtggta catttaaata atattttttg aaaacatctg caatttcttg
     7441 tgcgagtgct ttaataatat aactgaaata caatgtaatg actaacataa ttgttaacac
     7501 taaaatattt actactttta gaactgtgcg attaaataca attgtcagta ttgttatgac
     7561 taggctaacg atacataaaa taaagaaacc gttaaagact aaaattaagc gatcgattcc
     7621 ttgttgtaaa tcattgatat tacccaaatt aatatttaat tgatgcattt ttccaattgt
     7681 ctcgttaaaa tttttaatgc catcaaaatc tacagattct ttggtgaaga caatattaaa
     7741 aatgggttgt ttccacatgc tgtatccagc gattgcggtt aatataacaa caatgatttg
     7801 aataatcatg cttaccaatg aacgtttttt cttaccatat cgcttaccac gatgaatatt
     7861 cggttgttga aatgtctcat caaatggttc taatcgttga tttggatttg tcatgcaagg
     7921 tacctccgta taaagttctt ttctttactt atcaatatgt ctagttgtct gttataatga
     7981 tgaaaaatca ctgttcaatt gtagtcttaa atttgaaagt gaaaaagaaa atatcttcta
     8041 cattatatac tttttttggc aaagaatgta ggggatagac aatcattgga tacataaaat
     8101 atataaaggt aggttaagac attgattaga aaccgtgtta tgaatagcgt tgtcaataaa
     8161 tatttgcttc acaatcgctc gattatgttt aaaaatgatc aagatgttga aagatttttt
     8221 tataaaagag aaattgaaaa tagaaaaaaa cataagcagc cttcaacatt aaatgttaaa
     8281 gcaaatttag aaaaattgtc attagatgat atgcaagtct ttcgctttaa tttcagacat
     8341 caaattgata agaaaatttt atatattcac ggtggattca atgcactaca accatcaccg
     8401 ttccattgga gattgttgga taaaatcact ttaagtacat tatatgaggt tgtactgcct
     8461 atctatccaa agacaccaga gtttcatatt gacgatactt tccaagcgat acaacgtgtt
     8521 tatgatcaat tagtatctga agtaggacat caaaatgtcg tagtcatggg tgatggttca
     8581 ggtggtgcac tggcattatc ctttgtacaa tctcttttag ataatcaaca gccattaccg
     8641 aataaattgt atttaatctc accaatttta gatgcaacac tatctaataa agatatttcg
     8701 gacgccttaa tcgaacaaga tgcggttcta agtcagtttg gtgtcaatga gattatgaaa
     8761 aaatgggcga atggcctacc attaacagat aagcgcatat cgccaattaa tggcacaata
     8821 gaaggattgc caccagttta tatgtttggt ggtggacgtg aaatgacaca tcctgatatg
     8881 aagctatttg aacaaatgat gttgcaacat catcaataca ttgagtttta tgattatcct
     8941 aagatggtac atgattttcc aatttatcca attagacaat cacataaagc tattaaacaa
     9001 attgccaaat cgatagatga ggatgtaaca caaaataact aataacatat aaatgaccca
     9061 gttacgattt catgcaagat gatatttgac atgaaaacgt aattgggttt tgtttttgaa
     9121 aatatagcaa gggttatata gactaattgt taatgagctt ctgagtgaaa atcaccaata
     9181 agtgtattga tatgtgtgat gataaagtat tgccctttaa tttgataaaa ttgatctaaa
     9241 aaattatcga attgacgtgg aatatcacta agttctgcaa agttagctgt tttatcataa
     9301 ttgcgatgag caacctcatt ttgaaattta tcataatgtt ctaggaaatg ttgatcttca
     9361 agctgttgtt ccacaatagt cataatatta tcgatggctt gcttttgtcc ttgaacataa
     9421 gccatatgca cgccacgctt aagtagctga tttgctggtc cgttaatgta taaccctttt
     9481 tgtgcattta attctgttaa ttcaaactta acatcatcta ataaatctag taattcgata
     9541 tgaatctttt gttgtaatgc atctgtcatt ttaatgctcc tttgtgtgga attgctattt
     9601 tatgtcctaa ttgttcaggc caagtgatag ttgattgatt tttataatag tgtgctattt
     9661 gtgttgaaaa tgattcagga aaagcatcag aacgacgtgt gtgctgatta attcccatca
     9721 tcattacttc atttgttgat gctagtgtac catcttcttt agttaatgtt aaaaataaat
     9781 gcaaccgctt ataatcgtaa tcataaatat ataaagtaac agtaaataca tcgccaagag
     9841 acaattctga gaggtatgtc gtatgttctt ctagtgtaaa tagcgtatat gctaaatttt
     9901 cgcgttcttt taaagaaaga ccgtggctgt aattaaaacg attcacgacg tcactaaaaa
     9961 tgatattata atttgcatca tgcatatgat tgttatggtc aatgaattca gatgttacgg
    10021 tttgagtatg tgtaaaaagt tgtttgataa taaccatctc cttagtgact atcatactaa
    10081 aataaattaa gacatacacg ttatttaatt catgataaaa taatagagaa ttcgttattt
    10141 agggggagta tcgatggctt taaagatacg gtatcaaact acatatgaac cgtttaaagt
    10201 agttgatgat atcaaagaga taccaaaaga tgcgactatt gtttggtatg attttgatga
    10261 accgaatgaa caagaaaatg aatggtttaa agcacatttt aattttaatg atttagaagt
    10321 ggatgatgca attaacggta tgccacgagc taaatataaa tcttataaag attatcaata
    10381 cttagtattc catagtatta tggggtcaaa ctattcacca atagcactaa atatttttat
    10441 acaagacaat gtattagtaa catatcatca tcaaacgttg gaatcattaa ataaagtagt
    10501 ctataagtat atgaatacat tagatgcaga attagattgt gcagatgtcg tcatattaat
    10561 tttagatatg atggttgata aatatttcaa ctttgtatat gcacttgaag atagtgttta
    10621 tcattttgaa gaccgccatg tagatgatcg ctttaataaa atggtcatgg atagtgtctt
    10681 taagttacgt tcggatttga ttaaagtaaa acgtgtactg tttcctatgc aagagctaat
    10741 tgatacaatg aaacaaaatg gtgatttgat tatagataat aaacattcgc tatacattca
    10801 acatatcgat gaccacttga ttaagcaacg taatatcatt cgtactgctc aagaaatgac
    10861 aaacgaaata cgagaaaatt ttgagtcata cacgtcattt agaatgaata gtattatgca
    10921 agtactgaca ttagtatcag ttatattttc accgttaaca ttcatagctg ggatttatgg
    10981 tatgaacttt gtaaatatgc cggcattaca tttacattat ggatattata tttgcttagc
    11041 tgtaatgttt gttatagctg tcgtattaat catctttttc agacgtaaaa aatggtttta
    11101 aattagaaat gacgaaaagg taggtttaat catgagtgat tttcaaagag aacagagaaa
    11161 aaatgaacat gttgaaatag caatggccca atctgacgca atgcattcag attttgataa
    11221 gatgcgtttt gtgcatcatt ctatcccatc aattaatgta aatgatatcg atttgacatc
    11281 acagacgcct gacttaacga tggcatatcc ggtttatatt aatgcaatga cgggtggtag
    11341 cgagtggacg aaaaacatca atgaaaagct agctgtagtt gcaagagaaa ctggcttagc
    11401 gatggcagtt ggatcaacac atgcggcatt gagaaatcca cgcatggctg agacgtttac
    11461 gattgcgcga aaaatgaatc ctgaaggcat gatttttagc aatgttggtg cggacgtacc
    11521 agtagaaaag gctttggaag cagttgaatt acttgaggca caagcgttac aaatccatgt
    11581 taattctcct caagaattag ttatgcctga agggaatcgt gaatttgtga cttggttaga
    11641 taatatagcg tcgattgtat cacgagtgtc tgttccagtc attataaaag aagttggatt
    11701 tggtatgagc aaagaattaa tgcatgactt acaacaaata ggcgtcaagt atgtcgatgt
    11761 tagtggcaaa ggtggtacta actttgtaga tattgaaaat gaacgtcgtg caaataaaga
    11821 tatggattac ttatcatcat ggggacagtc tacagttgag tcattacttg aaacaacggc
    11881 ttatcaaagc gaaatttcag ttttcgcgag tggtggttta cgtacaccac tcgatgcaat
    11941 taaaagttta gcacttggtg caaaggcaac tggtatgtca cgtccgtttt taaatcaagt
    12001 tgaaaataat ggcattgcac atacagtagc ttatgtagaa tcatttattg aacacatgaa
    12061 atcaataatg acgatgttag atgctaaaaa tattgacgac ttaacacaaa aacaaatcgt
    12121 atttagtccg gaaatattgt catggataga acaacgtaac ttgaatatac atcgaggata
    12181 aataaaagag gaggcgaagg cctcctcttt tatttatgag tttggtgttt aactaaacca
    12241 ttgtatgaat cccataatga ctataacacc aattaaatcg attaagaatg caccaacaat
    12301 aggtacaact aaatatgctt taggtgagtt tccatatttt ttagtaataa catctaaatt
    12361 tgccatggca tttggcgttg caccaagccc atgaccgata aaaccaccta ccattactgc
    12421 agcatcataa tcttttccta aacctctaaa taaaattaaa acagcaaata aaatcataac
    12481 gacaacttga actaaaacga taataataag aggtatagca agtttataaa tttcgattaa
    12541 ttgaatgctc attagcgcaa gagataagaa aatacctaat gcgacatcgc caatttgatt
    12601 agtaattttt aaatctacaa tattaaaatt aaaactttca gagatatttc ggacaataac
    12661 agctacaaat aatgagccaa cgtaaattgg aacattaatc cctgtttgag ctgtaaacaa
    12721 atgactgaaa taacttccaa ctgccataca gaatacaacg attgtaaatt gaatgaagaa
    12781 tacttcagtg gcattaaatt tactatgtaa atgttcgtta tatgctactt ggctataatc
    12841 tttaaatgta tcgtcactat gttgaggctt taagttataa cgtttgatta aaaattttac
    12901 aactggacca ccgataagcc ctccaaatac aagacctaaa gttgcagctg caagagccgc
    12961 tgtcagtgcc gaatcaatac ctaaatcttg aattgtctta ccataagcag cagcattacc
    13021 atgaccgcct tccatagaca tggaacctgc tgttaatcct aacaaaggtt taatatttaa
    13081 tacttttgct agtgatacac caactatgtt ttgaatgact gaaatgatag cacaaaacat
    13141 aaagtataat agcaagactt tgccacctaa tttaaataat ttcaatgatg caccaagacc
    13201 gattgtcgta aagaatgcta acatgaagaa atcttgaatg aatgaagcat ctaatttaat
    13261 cttaaccatg ccaaatgaat ccaaagccgc aactaaaata gcaaagatta agccgccaat
    13321 cactggtgct ggtatacaaa tacgttttaa aaaattaacg tgattaacga tagccttacc
    13381 aagtaaataa aggatacaag ctaaacataa tgttgtaatt gcattaagtt ctatcatttt
    13441 caaatccccc ttgttgaata tccctctccc taaataagct tttatatgta agcgttataa
    13501 aagggcgctt ttaacattat acatgcaaca aatcaatatt tctagttttt gaaaataata
    13561 tcaataatat taatgcatct aaatttaata attataaata attctattgt tatattttat
    13621 aattaagaac aagttcaata agaggagatg aatcaaaaaa tatgatggat acaaagaaaa
    13681 acaatcaacc catccgcatg ttgacggcac gattgattga cttataagga gatagatttc
    13741 attttcatga tagtaatcaa tgaaagatgg catttatttc caagtatctt cgggaaacat
    13801 acaatctgat ttacgcatgc gagacacaaa tggattacct ttcactgtgt aacgtaaaga
    13861 tttatgtgtc caatcacctt tatttggaat accgattcgt ggactagcaa taatatcttt
    13921 aggatattta cgattcttag tatcaataga caatctacag tcatttaacg tagcgccatc
    13981 gatagcccgt ggaatgttaa atgccttagt ccattttcct gggccattcg ttacctcgta
    14041 gcctttctta cctctgttac ggaacatagc ggataaacct tcttctggtt caattgcgcg
    14101 gataagtacg ccttcaggta taccttcaga ttttgttaca aaattaatga gtaaatgcgt
    14161 atgcatgaca tgtgcataaa ttgtaccacc acgtttatat aatgacgtga ctttaggtgt
    14221 tattttaccg ccatagccat gagccgcacg atcattcaaa cctaagtaag cttccgtttc
    14281 cacgatgtag cctgtatacg tttgagtggt atcctgataa atcactttga cacctaataa
    14341 gtttttagca atttgtcttg tatcattatt aacgaaatcc ataatattct cctcgcaatt
    14401 atatgattaa tttaaataaa aatgaaaaag atatttttat tgtggtaaat gtttgataaa
    14461 atagtattgt taagaattat aaaaatgtaa agtatatact ttgttatgga atattttatt
    14521 tgcatagatt gagactcttt taaacgtgaa cattgcagta tgacatattt ttagtatagt
    14581 taacgaagcg ttgactttca aatgagtctc catatagatg tgtacttgta gcgggttttc
    14641 attttactag tgatgaaata taaagttcat tttccaaagt gattgacgat tgaaatgaag
    14701 tggcttgata atgaatcttg taaattataa cggtaacaat aattaaaaat ataaatatga
    14761 ttaacgaaag ggagattaca atggaacaat tacctcaagt aggttttatg aaatcattcg
    14821 ttttattttg gaaaaattat gtaaatttta aggggagatc aagacgtagt gaatattggt
    14881 atatggcact atggcatttg atacttatgg ttccagctgt gtgtgtgcta ataataaacc
    14941 tatttctatt ctttatttct atagcggcgg ataacgaaac attaatagtt atgagtatat
    15001 tgatgattat ccttacatct gtgtatactt ttgtctatag tttggcaacg tttattccaa
    15061 atttagcgtt gtgcgttcga agatttcacg atatatcaag aacaatgtta tttccaatga
    15121 ttatgacagt attcagtatt atattttata tagtattaca gattatcgat agttattatg
    15181 acagtgattt tacattaatg ccgatgggtc ttaatatttt gttagtactt ctttattttg
    15241 tttatttagg cctaacaatt gttatgatag tattcctatg ctttgacagt aagccggcaa
    15301 ataaatatgg cgaaagccca aaatacccgt ctacaattaa aaatcaacca gtgacatctg
    15361 aaacaccgga aaaagcgtgt acatctgaaa caccgaaaac accggaagaa acagataatc
    15421 aattgtaatg atataaatat ctcggcacaa tgatgctggg atattttttt acaaataagg
    15481 caataaaggc atggaaaaaa catatctagt gctaatgata cttaacatgc aaaaatggca
    15541 ctatttaaat ttttcaattt cagaaatttt aaaaataaat tcattttaaa aatattgcat
    15601 ttatagagaa tggaaatgtt atattaagat aaatacatat ttttttagga ggcaaagtat
    15661 gggacttgta attgaacatg ttacgaaacg ttttggcaag atgacggcag taaatgatat
    15721 ttcattaaag ttagaatcag gtaaaatgtt aggttttcta ggaagaaatg gtgcaggtaa
    15781 aacaacgaca tttcgtatga ttttaggctt aagtgaacca actgaaggac acattacgta
    15841 taacggtaaa aaactagata aaacaatgta caatcgtatt ggttatttac cggaagaacg
    15901 cggtttacat gggaaattga cagttgaaga agaattgaaa tatttggcaa ctttaaaggg
    15961 aatgtcaaaa acagaaatac aacagcaaat atcgtattgg cttgagcgtt ttgatattac
    16021 tgaaaaccgc aaaaaacgaa ttgatagtct atcaaaaggg aatcaacaaa aaattcagct
    16081 gttagcaagt atgttacata aaccagaatt actaatatta gatgagcctt tcagtggtct
    16141 agacccagtt aatgttgagt tattaaaaga agcagttaaa gacttaaatg attggggcag
    16201 tacaatcgtt tatagttctc accgtatgga gcatgttgaa gaattatgtg atgatgtttg
    16261 tattttagat aaaggtcaac ttgttgtttc tggtgatatc aatcatgtac gtgcatctaa
    16321 tggtaacaag aaagttgtca ttgaatcaga aacaacacta ccagatttaa caaacattag
    16381 aggcatcatt catagtgaaa acatgaagca aggtcttcag ttgacgattg agaatgaaga
    16441 cgtagccaaa gatatttatc aagttgtggc acatcaaggt tatgttaaac gattccaagt
    16501 tgtagaacca tcattacaag atatattcat tgagaaggtt ggtggcaagg atgcataagt
    16561 tttgggcgac atttactttg acatatatca aaaaaattaa agctaaatca tttgtaattt
    16621 ttatgattat tattgctgcc ttaatgattg gcctatctaa cattgataag attattaaca
    16681 tgtttgatga tggtccagat aaaattggtg tagcagcacc gaatgaacaa atctataagg
    16741 ttttcaagca acaggcgaat acatttcata gtgatgctaa gtttactaaa gtttctatag
    16801 aagatgctga aaaagaagta aaaaaacata aacttgataa agcttatatt atcaaggtga
    16861 atcaaaatcg cacgttacaa gggacaatca ttagtgagaa gcgagtaagt catgaagatt
    16921 cacaaaaagt acaagcactg cttacagcta ttcaaacaaa tatggtagcc ggggaattaa
    16981 atatcaacaa agaagactta caaaaattac aagcacaaag taaagttgat aataaggtga
    17041 tttctaatga tgaagtcgat aaagtatcag aaggacaaaa gattttcaac tatgctttag
    17101 cttatggcat tatctttcta atgttcttca ttgttttaaa ttatgcatct caaatagcta
    17161 tggaaattgc tagtgagaaa acatcgcgtg tcattgaaat gattattaca agtatttcac
    17221 caatccaaca tatttttgct aaaattttag gcgttatagc agttgccgtg actcagatta
    17281 ttttaattgt attgatggca gttatatgca tttttgcctt tgacttaaaa gacttgctac
    17341 aaggttttaa cgtggaaatg aatcaattgt cttggcaaat tattattgtc ggaattataa
    17401 gtagtattgt tggaattacg gcgtatgtat tacttgcagc catcctcgga tcattaacat
    17461 ctagaataga agatttaaat caatctttga tgccactgac attacttggt atgattgcat
    17521 tttatatcgc gatattcacg ataaataatc ctgatggaca acttgcttta atcacaagtt
    17581 atattccgtt tttagcgcca tttcaattag ttgtacgcgc tcaaacttca gggcttcaaa
    17641 tatatgaggt catcttaagt tctttaatct caattgtaat ggtagctgtc ttgatatgga
    17701 ttgcaattaa aacatataaa gattcagtac ttacatttga acgtggctta tttaattcaa
    17761 tgaaacgtgt atttaaaaaa taaaattgat gagagtgaca cacatttatc atataaaatg
    17821 atgatgaaag cgtgttactc ttattttttt atatttagct atagaataat gtctaaaaaa
    17881 gtgtacaata tgaatgtgaa aacgaacttt ttaaaatggg aatgtgaagt aagaagaaat
    17941 gtaaacaaat gatatgtagc cgatagatta ttgatagcaa atgtgtgatg gagtgatttc
    18001 atgttatatc cgatttttat atttatatta gctggcttat gtgaaatagg tgggggatac
    18061 ctgatttggc tgtggcttag ggaaggacag tcttcacttg ttgggctaat aggcggtgcg
    18121 atactcatgt tatatggtgt cattgcgaca tttcaatcat ttccatcatt cggaagagta
    18181 tatgcagcat atggtggcgt atttatcatc atgagtttga tttttgcgat ggtcgtagat
    18241 aaacaaatgc cagataaata tgatgttatc ggtgcaatca tttgtattgt aggtgtttta
    18301 gtaatgttgc tacccagcag agcttgaaca cttgtggtta gaagggagaa gtaaaaatga
    18361 tagttgaagt tgagcaccaa agacaaggtg tagatttaat caaaattgat aatgatgaaa
    18421 ctaaaattat ttttacgaat tatggagcaa gagtagtgtc ttggaaatat catgataata
    18481 atattgttct tggaaatgtt gttgaagcgg atgaatttta tcatagtaat ccttttaaat
    18541 ttggtgcttc tataggaaga tatagcggta gaattgataa tgctaaattt aagttgaaag
    18601 gtaaagaata tcagttagaa aaaaacaacg gtgaacatca tttacatggt ggatgtcatg
    18661 gtttggacaa taagttattt gattatgaaa ttagaaatga aattgctcaa attaaagtta
    18721 tatttaagac ggtgttgaaa tcagctgatg atcatttccc gggtgatatc gatgtgacga
    18781 ttacacatat ttatgatgcg gatcaccaat ggtcgattga atacgaagca gttgcatcag
    18841 aagacacatt gtttagtcct acgaatcatg tgtacttcaa tttgaatcga gataataatg
    18901 tcgttgataa tcatcgcatt tcaagtaatc aattagatat gtatgtatta gatgagcgaa
    18961 atattgttac aggtgatatt ctagatttac atgaagtgtt tgaagataac aagataaaat
    19021 tgagtgatat ttttacatca caacatgcgc aattaagcca acaaatgaca cgttttggtg
    19081 gtctcgatca cccatttaca gttggtgaac ataaaatgta tgttgaaaat catgaattta
    19141 tgttagaggt cgatacagat atgcctcatg ttgtgttctt tacttttaac cagcctgatg
    19201 agtgggatag tccatttaat atttataaac cacattctgg ctttacatta gaaacacaat
    19261 ttttaccaaa tgatattaat ttatatggtg aaaaagcacc gtcaatttta aaagctggtg
    19321 aagtgttcaa ttcgaaaaca agtttccgta tcgaagaaaa agtagaaaat gattaaaagt
    19381 atcggcattt atataatatt tatgttattt ggcggaattt tgaataggtt atcaagaatc
    19441 gtatcaaatg aagtgacatc attaatatac agtttgaaat aaagaaaata tataacataa
    19501 aaaaggaatc aatagcatca gtggttatag ataagactat tatacaaacg atgtgtaagt
    19561 tgattccttt ttgttttgaa aagttcattt aacacttata atgaaaggta attatttcaa
    19621 tgttctgtta tacatttttt gaagtttatc gcgtcttgtt ggtgacagaa atggattgtt
    19681 tagtgcatat cgtaacatat cttgattttg tctatcatta taataaaggt cattaagctg
    19741 ttgtacagat aaacgatgtt cgattggtgc ttcttgtatt aagtaaagct gatcatttgg
    19801 agatacatag ccttgtttta atacccggaa atagaaacct gttttaccag atgtagacat
    19861 gcgcttcact aaatcaggaa tattatattt tgcttgaatt ttccagcatg gttctctaat
    19921 ttcagaaacc tcaattaacg cttcacctaa acgatattga ttaccaaagt aaacatcaga
    19981 ttcgtctaaa taatcaaatg ttaaattctc tccaaacatc gcatgagtag gtagtgtagg
    20041 taaatcatct tgatacagtg cataattatg cttactaaac ccacaaactg ctttatgtgg
    20101 tccaccatga tctttataag cctgttcatc accagtgaaa ccagttgaag ataaccatgt
    20161 tgtttgtgaa atcttagttt tatctaaagc agagcgcatc ggtcttttcg agctataagg
    20221 caaatcttgg atttttcctg tcgaaattgc gtgaattgat atcatatcat tctccttaac
    20281 gagtattatt taaaatatat tgtactataa aacattttta gaattgtaaa ttctgattga
    20341 tatgtttaaa aaaatgttag aaataagggt atgttaattt atacgagtca tgtaaaatat
    20401 gaattgaata gattacataa atatgggagg ctaccttcat gaaagatgtc aaagcactca
    20461 agttaatgac attaaatgat gttttaagtc aaatcaatgg tgacatgacg ctgggaattg
    20521 gtacaggaag tacaatggaa ttactcttac ctcaaatggc gcaactaatt aaagaacgtg
    20581 gttacaatat tacaggtgta tgcacttcta ataaaattgc atttttagca aaagaattag
    20641 gtattaaaat atgtgaaatc aatgatgttg atcatatcga cttagcaatt gatggtgctg
    20701 atgaagtaga tccatcttta aatataatta aaggtggcgg tggtgcgctg ttcagagaga
    20761 aagttataga tgaaatggca tcgcgttttg ttgtggttgt cgatgaaacg aaaatagttc
    20821 aatacttagg tgagacgttt aagttaccag tagaggtgga taaatttaac tggtatcaca
    20881 tattacgtaa aattgaatca tacgctgata taaaagtaga acgtcgtgta aacgaagatg
    20941 tcgcatttat aactgataat ggcaattata ttttagattg taagttacca aaaggaattg
    21001 acccatataa gttccatgag tatttaattc atctgacagg tgtgtttgag acaggatact
    21061 tcttagatat ggcagatcaa gttattgtag gcactcaaga gggtgttaaa attttagaaa
    21121 aataatatat agtatagaaa agcgcttgct agcatcatct ttttaagagg tactagcaag
    21181 cgctttgtta ttatttgttt ttatctgaag attgttcttc agttttttcg atatgttttt
    21241 ctgcatcaat tacaactgat gaacgtctgt cttctttata agtcgcaggt tcttttaatg
    21301 attgctctat ttcatcttca tttgaatcgt ttgttaaatc gtcactttgt ggtgcttgtt
    21361 taactgattc tgctgattct actgactccg aagtgatgtc atgatcttca tcatgattag
    21421 attcagtttg attacctatg tgatgttgag gttctgtttt ttcagtacct tcatgaatgc
    21481 ttggttcgtc agccactgta ttttcagtat catttttagc aacaccaacc gttgatgcag
    21541 tagctacacc tgttttttca gcatctttaa tatttgaaga agatttttca gcctcagtat
    21601 gattagtttc ttcatcgtca ttgacatggt ctaaataatt attaggctca agttcttcaa
    21661 ggtttcgtct tgttgttaaa tatgcaatac ctcggataat taagcttaaa ccaatgtatc
    21721 ctactgcaac gattgctgtt gaaatggcga tgactttgat tgaaaataga tcgccgattt
    21781 cttcgctaaa caagaaaata agtccgaatg tcattgaagc atggaatgtc gttgcaatat
    21841 aaattgtacg tcctttagtc gctctaatta attcaccaag aatcatagag aatgagaatg
    21901 tataaaggaa gttataagca gcaaattctg taccataagt tgtatttgct gagaaaactg
    21961 aatacatcaa accaacaaca atacttgcaa agaacgtgtt cattttcgtt tcaacaatat
    22021 tttgtaagta tgaacggaat ccgaattcta ctacgaacgc catcagaata tgtccaatca
    22081 gaatgtgtgt aataggtact gataagcctg ttgattgtaa taaaataaag ctatctgcaa
    22141 atgtattaaa gctgtacata ccaataatta gaataattaa aggtaaaatt aaagctagca
    22201 atagacgttc aattactttc aggctgattg agaattttaa acctgcaagt tggacctttt
    22261 tatatttgaa aacgagtata caaatgattg cagcaattaa tggtgctaga tctgtaactt
    22321 caaatataaa gtgtttgaca ccaattatag actggaaatc tctgagcata atggataacg
    22381 ccattgtaat gacaaagaag acgaaaatcg tcattgccca ttgaaaacca gaaattttat
    22441 tgttcttcat tatatgtaac ctccattagg taacaagcaa aatatctttt agtaacaatt
    22501 atacatatta caagccgaga gataaattgt ctatgtcaat attaatgaaa atgtaatagt
    22561 cttgacgtat tttattaaca taaatcagct aagtattcgt tattacatag aaaatagggg
    22621 taaaagtaat gtagataagt atttttaaga tacggattta actatgaatt gaattcaaaa
    22681 tttcctatac gttatacttt aattgttaat aagccacata aataagaggg ggaaatgctg
    22741 tgtacaagca aggtgaacca aatttatgga ctggaaggtt agatagtgaa acagacccga
    22801 aaaaatttag acattttcaa acagtaacat ttgaagattt gtctaagctg gaaaagagta
    22861 gtatgccatc aggggtcggt atattaggct atgctgttga caaaggtgtt gctttaaaca
    22921 aggggcgcat tggtgcaaaa gaaggaccag atgcgattaa acaagcattt gcaggtttgc
    22981 cggatttgaa tcaatgtgaa actttagtcg attacggaaa tgtttatcat gatcatgagg
    23041 aattaattga tactcaaaaa gaatttgcta tgcttgcagc gaagtcaatt gctaatcata
    23101 gacaaacatt tttattaggt ggtggacatg atattgcgta tgctcaatat ttagcaacac
    23161 gtaaagtcta tccaacacaa tctattggtg tgataaatat tgatgcgcat tttgatacac
    23221 gtgctgaaca acaatctaca tctggaacga gctttagaca aattttagaa gaagatgaaa
    23281 acacagatta tttagtgctt ggtattgctc aaggtggtaa tacgcaaagt ttatttgatt
    23341 atgctaaaga gaaaaagatt gattatgtct ttgcagatga attattgagt cacgtatcac
    23401 caacaattaa agatatgatt gaacgttttg tacatgaaca tgatgtcatt atgtttacga
    23461 tttgtatgga tgtcattgat agtgcgtttg cgcctggggt aagtgcgccg gcagtgttag
    23521 gtttgtatcc acatactgtt cttgaattag caaaacgtat tattccaagt gataaggtgt
    23581 cttcagttag tattgctgag atgaatccaa catatgacgc tgacaataga actgctaagc
    23641 tcgttgctaa tttagtgcat cattttttaa aataattaaa aatatttata tgtgacgaga
    23701 tttgcattgc agggcagaat gtgagtattt cttaatagaa gttatattaa cgttgatata
    23761 tttgaaaaat aattgcagta agaagactct taagttgtag tcatcttact gcttatttat
    23821 gtttgttgaa atagcaaaaa taacaactgt attgtttgaa aagttctctt attatataat
    23881 aattaattaa aattttaacg gagttcaaga ggccttttta taatgacacc ttatttgtaa
    23941 aatgtcatat gtggtttagc ctctttataa taatttaatc tgttctcaag tgtgccagta
    24001 tgtaattcta gcttatgacc atcagggtcg gtaaagtaaa ttgattgtct atctctaata
    24061 tctctaactc ttccttctaa aatattcacg ttattatctt ttaacctctg atgccaatat
    24121 ttaaattcgc tgtcatctat agtgaaagct atatgtgtat atgaaaagtg aatttcatta
    24181 cgtggtatat ctttttcttc atttaaagca atccataggc ctgcaagctc aaaataagca
    24241 gtttttttac cagtcaatag caatttccca agtaaaatat ctctataaaa atgtattgaa
    24301 tcgtttaaat ttctgactga aaagcatata tgattaatag attttaacat ctgcttcact
    24361 tctttcttag aatttttata ataaattatt tttgtatcga tttttgaaat gacgtcatta
    24421 atttttatac gatgatctgc gcaactgcat aagaggctct aataacttaa gttaaagagc
    24481 ctctaaaagt ccatataaat aatcttggca ttgttgtacg aatgtatgta cttgttcgga
    24541 gtgtttgcgt tttttatggt aattgatata aatttttcgg cctaagtttg gacgaatttt
    24601 tttatattct aaattagacg tgtgaaatga ttggtaataa aatctcggaa tgatagcgta
    24661 acctaagccg agatgaacaa atccaactgc tgattcgaat cgatctgttt ctacaacgac
    24721 atttggacga atatttcttc tattaaaata gtcatctaag tgtttgcgca cttgagaatt
    24781 tttgtttggt aatatgagtg gcaaattttc aacatctacc caattttgat ttttaaatgt
    24841 ttccttgggt gctaataaaa tgtaagattc ctcatataaa ggaatggatc ttatatcttc
    24901 gtgggttatt ttttcatttg ttatagctaa atgaatatta aaattcagta attgctctat
    24961 agattgatgt ttatcatgta tttcatataa acgatattgc tgttcagggt agtcggaatg
    25021 gtgctttcga attaaattcg caatccattg attcgtagat tcaagagtcc caatttttat
    25081 ccttggttct gatgtaacgc ttaaatcata cattttttcc atcgtggatc gatattgttg
    25141 aactaattcg ctcgcataac gataaaactg tattcctttt tcggtaatct tgatgtcttt
    25201 tgttgaacgt gtaaataagt cataacctaa atctgcttcc atttttttaa tcgtagcagt
    25261 taaagatggc tggctaatat gtaaaaattg tgcagcttta gtaaaactat tatatttcac
    25321 gatagccaag aagtattcta actgaataat tttcatgtgt tttgctcctt ttatttatag
    25381 attcaagcta tggataatta gtttatttat atttgttggc tatattttaa catactataa
    25441 tgaatattga agataataat gtaagcgttt taaatttagt ggaattaact tcttaaaaga
    25501 acaggagcga attttatgag aaaaattcaa gcaaaaaaag gtttgagtat tgagtgtaaa
    25561 ggttgggaac aagaagcagt acttagaatg ttatacaaca atttagaccc agaagttgct
    25621 gaaagaccag aagatttagt tgtttatggt ggtattggaa aagcagcacg taattgggaa
    25681 gcttttgaag caatagagaa aacgttacga gaattagaat cagatgagac aatgttagta
    25741 caatcaggaa agccagttgc tgtatttaaa acacatgaag aagcgccacg tgtattaatt
    25801 tcaaattcag tattagtgcc tgaatgggca aactgggatc attttaatga attagataaa
    25861 aaaggtttaa tcatgtatgg acagatgaca gccggtagtt ggatatatat tggttctcaa
    25921 ggtattgtgc aaggtactta tgagacattt gccgagctag gtaatcaaca ctttaatgga
    25981 gatttagcag gcactgttac actgacagca ggtttaggtg gtatgggtgg tgcgcaaccg
    26041 ttagcaatta ctatgaatca tggggtagca atttgcgtgg atgttgatga aacacgtgtt
    26101 gataagcgaa ttgatacgaa atactgtgat gttaaaacag ctgatttaga tgaagcatta
    26161 aaattagcag aagaggcgaa agaacgtgga gaaggattat caatcgggtt agttggaaat
    26221 gctgtagaca tccatcaagc gattctagaa aaaggattta aaattgacat tattactgac
    26281 caaacaagtg cacatgatcc gctaaatgga tatgtgccac aaggatactc tgtagaagaa
    26341 gcgaaagtat tgcgtgaaaa agatccgaaa aaatatgttg aactgtcaca agcttcaatg
    26401 gcaaagcatg ttgaattaat gcttgaattc caaaaacgtg gcgctgtagc atttgattat
    26461 ggtaacaata ttcgtcaagt agccttcaat aacggagtga aaaatgcttt tgacttccca
    26521 ggctttgtac cagcttacat tagaccatta ttctgtgaag gtaaagggcc attccgcttt
    26581 gctgcgttga gtggtgatcc aaaagatatc gagcgtgcgg atgaagaaat gcgcaaactt
    26641 ttcccagaaa atgaaaagtt attaagatgg cttgatttag ctgaagaaaa aatttcatat
    26701 caaggactac catcacgtat tgcttggtta ggctatggag aaagagcgaa aatgggctta
    26761 gctttaaatc gtcttgtacg tgacggtgaa atttcagcac caattgttat tggacgagac
    26821 catttagatg ctggttcagt tgctagtcct aaccgtgaaa cagaaagtat gaaagatggt
    26881 agtgacgcgg ttggtgattg ggctgtattg aatgcactca ttaacacagc tgcaggtggt
    26941 tcatggattt cattccatca cggtggcggt gttggcatgg gatattcact tcatgcgggt
    27001 atggttgttg tagcagatgg atcagagcgt gctgaaagaa gattggaacg tgtattgacg
    27061 actgacccag gtatgggtgt tgcccgacat gttgatgctg gctatgacat cgctattcaa
    27121 acagctaaag aaaaaggtat tcatattcca atgattgata aagcaggtga taagtaatga
    27181 atgatttaat aattaatcat atagcagaat taattttacc gagatcaaca gataaacctt
    27241 tgaagggtaa ggaattagat gaattaaatg ttgtgaaaaa tggtacagtt gtcattaaag
    27301 atggcaaaat tgtatatgct ggaacacata cagatgacta cgatgcgact gagacgattg
    27361 atgctagtgg gaaggtagtg tccccagcat tagtagatgc acatacccat ttaacatttg
    27421 gtggatctcg agaacatgag atgtcattaa aacgtcaagg taagtcttac cttgaaatat
    27481 tagaaatggg tggtggcata ttatctaccg ttaatgctac tagagaaaca tccgaagatg
    27541 acttgtttaa aaaagcagag catgatttgc ttactatgat taaacacggc gtgcttgcag
    27601 ttgaaagtaa gagtggttat ggattagata gagaaaatga actgaagcaa ttgaaagtgt
    27661 ctaatcgttt agctgagaaa tatgatttag acatgaaaca tactttccta gggcctcatg
    27721 ctgtacctaa agaggcaagt tcaaatgagg catttttaga agaaatgatt gcgttacttc
    27781 cggaagtaaa acaatatgca gactttgcgg atattttctg tgaaacaggt gtatttacaa
    27841 tagaacaatc gcaacattat atgcaaaaag ccaaagaagc aggttttaaa gtgaaaatac
    27901 atgcggatga aattgatccg ttaggcggac tggaattagc aattgatgaa caagcaatat
    27961 cagcagacca cttagtagct tctagtgata aaggaaaaga aaagttgcga aatagtgata
    28021 ctgtagctgt cctcttacct gcaacgacgt tctatttagg taaagaagat tatgcagatg
    28081 caagaggcat gcttgacaat aacggtgcga ttgcattagc aactgattat aaccctggta
    28141 gtagtgtcac aaacaactta caacttgtta tggcgattgc agccttgaaa ttaaagctat
    28201 cacctaatga agtttggaat gctgttacgg tcaatgctgc taaagcaata gacattaacg
    28261 cgggtacaat taacacaggt gataaggcta atttagtaat ttgggatgca ccaaatcatg
    28321 aatatattcc atatcatttt ggtatcaatc acgcagaaaa agttattaaa gacggtaagg
    28381 tcatcgtaga taacacagta tcttttaaag cataaaataa ttagaaagct ctaagcattg
    28441 gtttaaacta atgtttagag ctttttgtgg tttgtaaaaa caagtatatt cgagaacacg
    28501 tttttgtata gagtctcaat tataaaagtt agaatagttt gacaacagtg ttacgatatt
    28561 attaaacaac tgtctatcat taacgaaagc aattaacaag agtacattgt tgtgaaatca
    28621 tcgtttaata gtcaaagatg atcatcgtag ggtagacaac atatcattga cttaaggaag
    28681 gggaatgacc gatgggatta aaagatcaag ctgttacctg gagaaggtat tttcatcaat
    28741 ttccagaact atccgataaa gaatttaaga ctacacaaaa aattaaagat attttaacag
    28801 aacatcatat tagaatatta gacttaccac ttgcgactgg gcttgttgct gaagtgggac
    28861 aaggtctgag ttgtatagct gttcgggctg acattgatgc tttaccgatt caagagcttg
    28921 ttgaacagga ttttaaatct gaaaatgaag gtgttatgca tgcgtgtggt catgatattc
    28981 acatggctag tattttggct acagctgtaa aattaaaaga aattgagggc actcttactg
    29041 ggcgtgttaa attcattttt caatcagcag aagaactagg acatggcgct tttaaaatta
    29101 ttgaaacaca tgcattaaaa gatgtacaag cagtattggg ttttcataat gacccatcgc
    29161 gttcggtagg tacatttgca atcaaaacag gggcaattac atcagcagta gatcgttttg
    29221 agtttcatat taaaggcgtg ggtggtcatg ctgcaaagcc agaacaatgc aacgatccag
    29281 ttattgtgtt ggcgcaattg attaatagta ttcaatccat agttagtaga aatctatctg
    29341 cgtttgatga agcggtagta acaattggac aaatatcatg tggtaatact tggaatgtca
    29401 ttgctgatca tgcgtatgta caaggcacag tgcgtagttt tgatcctgtg gtacgaaagc
    29461 ttgtagaaac acgattacaa gatattgcgg acggattggc tcaagcatat aatatgaaaa
    29521 tcaatttaaa ttatacgcat ttacctggcg ctgtgatgaa tgatgaggca ttgacgcata
    29581 aagcaattgc agtagcccaa catgttggat ataaggtgga aatgatggaa cagccgctta
    29641 cgattggaga agatttttca ggttatagtc aacattttcc aagtgtattt gcgttaattg
    29701 gttctcatag tgaatatgat ttacatcatc ctcaatataa accggatgaa aggattctgg
    29761 aaaaagtacc tgagtatttt gttgaatttg taaagagact attgcatgaa taattcgagc
    29821 ttgtttttat aaagaagaaa gtaattttta gtgtaatcat gattaagttc ctaggaagat
    29881 tgggtaaata ataaccatta agcgaataag gagatgtaaa aaaatggcag ctcaagatcc
    29941 tagaacaaaa tttaaaacaa cggattatga aaaacaagaa caagaggtac cgggtttaca
    30001 atctgaaatg acgccagcac ccgattgtgg cgaaacgtct taccaaggtc atcaacgctt
    30061 acaaggctat aaaatattag tcacaggtgg tgactcagca atagggcgtg cagcagcaat
    30121 tgcgtatgct aaagaaggtg cagatgtagc gattaactat cttccgagtg aagaacaaga
    30181 tgcacaagaa gtacgccaag tgattgaaga aagtggtcaa aaggcagtgt taattcctgg
    30241 tgatataaga gatgaacagt tcaactatga ccttgttgaa caagcatatc aacaattagg
    30301 tggcttagat aatgttactt tagttgctgg acatcaacag tatcatgatg atattcatgg
    30361 atttacaact gaggcattta cagaaacatt tgaaacgaat gtctatccgc tattttggac
    30421 agttcaaaaa gcattggaat atttaaaacc gggtgcgtca attacaacga catcatctgt
    30481 acaaggttat aatcctagtc ctattcttca tgattatgcc gcttcaaaag cagcaattat
    30541 ttcattaact aaaagttttt cagaagaatt aggaccaaaa ggaattagag tgaactgtgt
    30601 agcgcctggt ccattctggt caccattaca aatttctggt ggacaacctc aaagtaaaat
    30661 cccaacattt ggtcaaaaaa cacctttagg tcgtgcaggt caacctgttg agttatgtgg
    30721 aacttatgtg ctattagcgt cagaagaatc aagttataca accggacaag tgttcggtgt
    30781 tagcggtgga gtgcaaatag attaggtttg tatgaagctt ttaattgaac aacataatat
    30841 taatttagtt acttcaatcc attaaattaa aatgaattgg tgaagtgtat tttaaatagt
    30901 cggtaaaggt gtgtatcttt atcggcttat tttaatactc tgtcataagg agcgtttgct
    30961 cagggtgtgg attataagtt ttaagcagaa taaactttct taatgaggat gtcaggacaa
    31021 caacgaaaaa gttaaaaact gttgcgggat tgggtttagg gacggttagt ggaagaaagt
    31081 taaattcatt aacaagtgaa atgcctaagg ctagatatta aacgtcagaa cggacaaaga
    31141 ataataggat atgttttcaa ttaaatagcg aagtatttat atgttaagat gatgtaaatt
    31201 acatagaaac gctacccgta gaagtactga tcatagaaac gctgataaac gtaaatttgc
    31261 aaactcgata ttaaacaaag gggttgttat tatggcatta atagtatcta gagctatatt
    31321 atttttgatt ttagcattag cttttggaat agcgactgtt tttcctgaac aaaaattacc
    31381 attatatata gtattcacat tatttgtagc agttgcgatc gtttcgaatg tatggtttac
    31441 acatcaaaac gttaaaaagc atatcattaa gtttaacaaa tacgtcgcat actttttatc
    31501 ctttgtctca gggttacttt acattggatt tgtaatttat acattaacta tagggagcta
    31561 tgatgtttta ccaattatta tatttgcaat aagtatcgta ttggttcaaa tattagaaaa
    31621 tatactggga atcagtataa aaaatacgcg tctatcaaac aaacagttga aagtcatctc
    31681 caagcaacgt gttcagcaat ttaaagtcat atcattaata ttagtaagtg tgtctatcgt
    31741 gttcattttt attgcattaa tgcttaaatg gtggctttta cttgttatta gcattattgt
    31801 tttgattatt attacagtag caatgattgt ttatgaaaaa tattatacga ctcctgatta
    31861 atttatgatt gacgtactaa ggattatctt gtaacgtaaa cgtaaataag catcacttat
    31921 ctagagaggt ggagggactg gccctatgaa gcctcggcaa catctcgaat gtgccaattc
    31981 cagtaaccgt aatggtttga agataagcag gtaaagcaca tgaaaacctc tttcttcatc
    32041 gtttgtgaga aagaggtatt tttaattgga aagcaggtaa aaaggatgga agtacataaa
    32101 aagagcaatg cttgggcatt attccccttg ttattatttg tggcgttgtt tttaggcgta
    32161 ggtattatca caggtgattt tacttcaatg ccattaaatg ttgcaattac gataacggta
    32221 attgtggcgt tattaatgaa tcgaaaagaa tcatttgcta aaaaagttga ggtcttcacg
    32281 aaaggtgcag gccattcaaa cattgtttta atgatgttga tttttatttt agcaggtgca
    32341 ttttcaaata cagctgaaaa aatgggtggc gtaaagtcga cagttaattt aggactatca
    32401 ttaatcccag aaaatttaat tattgtagga ttgtttgtga tttgtatgtt tgtttctata
    32461 tctatgggga cgtctgtagg aactgttgct gcaatagcac ctgttggtta cggatttgca
    32521 caagcgacag atgtaccaac tgcgctagca atggctacag ttgttggagg tgcaatgttc
    32581 ggcgataatc tatcaatgat ttcagataca acaattgctg cggttagaac acaacatacg
    32641 aaaatgaaag ataaattcag agtaaacttt aaaattgtat tgccaggtgc tattttaacg
    32701 attattattc tgtatttctt aacgaatggc atttctttaa atcatgctaa aaactatgat
    32761 tataatctaa ttaaagttgt accgtatatc ttagtattgg tattagcatt attaggtgta
    32821 aatgtcatta tcgtattgat tggcggaaca ttattagccg gaatcattgg tctcatagat
    32881 ggttcattcg gttggatggg gctactagat gcagtgtcca aaggtattat aagcatggaa
    32941 gatattgcta tgattgcgtt gctaattggt ggtttagtcg gtattattca acacaatggt
    33001 ggtattgaat ggttgttgca atttgttcgc tctaaagtga aatcaaagcg tggcgcagaa
    33061 ttaggaattg ctagtttggt aagtgtcgca gatattgcca cggcaaacaa caccatctct
    33121 attattatgt caggtccttt agctaaaaat attgcagatg aatatgatgt agattcaagg
    33181 aaatcagcaa gtattcttga tatatttgga ggctgcttcc aaggtttatt accatatagc
    33241 ccgcaagtca tttcagcggc tggtgtagct ggtatttcac cgttaatgtt attcccatac
    33301 agcatttatc ctatattatt aggtgtatgc ggacttattg cgattttatt taattttcca
    33361 aagctaaaaa agaacactag tcaagaaatg aaacattaat gcttaacaat atatcgatgt
    33421 agccaagtaa ggtggcttgt tatattaaca gatcatgttg tactcaccgt ataacgctaa
    33481 tcttttaaaa atgatgatca tctatgtttg gaactgagca aaaatacgtt aaaataaaag
    33541 taagagcgaa agtgggtgga caacagtggc aaaatttaat gtagagaatg aacatgtcga
    33601 agttgaaatt gaaaagcttt ataaattttc tccagagtta gtatacgaag cttggacaaa
    33661 gaaagatttg ttgaaacaat ggtttatgac atcagccaga acgaataaag agattgaagc
    33721 ggacgttaaa gaaggcggta aatatcgtat tgtagatcaa caacgtaatg gcaaagtaaa
    33781 tgtgattgaa ggcatttatg aatctttagt tatggatgaa tacgttaaga tgactatcgg
    33841 tatgcctgga ctaagtgaaa cgcaagatgt cattgaggta gaattttttg aacgtgaaac
    33901 aggtggcacc caaatgctat tttattatcg ttcattagtt gaaaaagaaa gacgatttac
    33961 taatttagaa tacaagcaaa agaaaaaaga atatcatgat gccatggtgc atggtttcga
    34021 attgatgttt gataagatgt accacgttat cgaaacatca acacaacaat aaaatgataa
    34081 aaggaagtta tgataggctc aaggccatta aatcataact tccttttttc taatttgaaa
    34141 tatcattatg tagtaaatac tcattatatt ttcttacaag tgtttcaaag aatatatgat
    34201 aactgatgaa gctagaatag tctgtaccat cagttaagta gaatgtgtta ctggatacat
    34261 agacgttgta atttgctttt tgagcaaggg tattatcttt cattgtagta acagaaataa
    34321 agtatttttg tctaagttga agcaagtttg ttacttctaa taattcatgt gtttcacctg
    34381 ataatgaaat aataaagaat aaatcattag gttcactctt gttcaaaatc attttaagtt
    34441 catgttcatc atgaagtaca atgacatgat tatgtaacgt taacaatata cgctgtgcct
    34501 ctgaagcaac gtttaactgt gcacgccctg taccatacaa atatattgtt gaagctgtat
    34561 gcattttttt agtaagaagc tggtaatcaa cgcgttctaa ataactgaat gtactatcga
    34621 tttcttgttt gaaactatcg atagagttag aaggtaattg gtgtgtctta ttactttcaa
    34681 atttaaggta cgatttaaaa tcactataac cgtcaaaacc taatttgcgt gtaaatctgt
    34741 gaatggttgc attcgaggca tgtgtgaatt gcgctaaatc ttgtattttc atatttttac
    34801 attcatctat atgtgtattg ataaagtgag cgatatgtaa atcattatca ttaagtttat
    34861 caaagtttcg attaatgtgt tcatctaaaa acatattcaa cctccttgaa aataaatgtg
    34921 taatagatcg ttattacatt aaaatcttgt atgtcattat atatatttgt gtattgatat
    34981 aggtttgatt atgcgtttaa aacgaatgta ttacaaatac aacgatgtca tatataaggt
    35041 cgtgaaaaaa ttttcactat atgaaaagat aatctgatta tacaaaatac tttcaaaata
    35101 cgtcaatgtc gttgtaggtg tgtaagcgtt tactatatat tatatttaaa tagtaagaca
    35161 tctatttata aatgaatcta attagatgta ttgttaggaa agatgatatg gagggaatag
    35221 aagatgaatg cgataaagcg tttcggtagt gcaatgattg taccggtatt aatgttcgct
    35281 ttctttggga tagtgcttgg ctttgcgaca ttatttaaaa atcctacaat catggggagt
    35341 ttagcagatc aacatacgtt ttggtttaaa ttttggtcag taatagaatc tggtggctgg
    35401 gttattttta cgcatatgga agttgtattt gttgtaggtc taccactttc tttagctaaa
    35461 aaagcaccag gtcatgcagc acttgcagca ttgatgggtt acttaatgtt taatacattt
    35521 ataaatgcca ttttaacaca atggccacat acgtttggcg ctaatttaga aaaaggcgtt
    35581 gaaaatgtac ctggtttaaa atcaattgca ggtattgcca cattggatac gaatattcta
    35641 ggtggtatta ttatttcagc tattattact tggattcaca atagatacta tagtaaacga
    35701 ttaccagaaa tggttggtgt attccaaggt ttaacatttg tagttacgat ttcatttttt
    35761 gtgatgttac cattagcagc aattacttgt gttatttggc caactgttca gcacggtatt
    35821 ggttcaatgc aacactttat cattgcttca ggctatatcg gtgtatggtt gtatcacttc
    35881 ttagaacgtg tattaatacc aacaggatta catcacttta tttatgcacc aattgaagtt
    35941 ggaccagtag tagtcaatca cggcttgaaa gctgaatggt tacaacattt aaatgagttt
    36001 gctaagagta ctaagccatt aaaagagcaa ttcccttatg gctttatgct tcaaggaaat
    36061 gggaaagtat ttggatgttt aggtattgct ttagcaatgt atgctacgac acctaaagaa
    36121 aatcgtaaaa aggtggcggc actacttatt cctgccacat taacagcagt cgttgtaggt
    36181 attactgagc cattagagtt tacattctta ttcatcgcac catacttatt tgttttacac
    36241 gcagttttag cagcatcaat ggatacttta atgtacgcat ttggtgttgt aggtaacatg
    36301 ggtggtggct tgttagactt tatttcaaca aactggttgc cattaggtaa agaacattgg
    36361 ggtacttatg ttgcacaagt tattatcggt ctgatatttg tagcgattta cttcttctta
    36421 ttcagattct taattttgaa atttgatatt ccattacctg gacgtaagaa aacagaagag
    36481 gaagttaaat tattttctaa gcaagattac aaaaataaaa aaggtgatag cgtagattct
    36541 aaacgtgctt catctggtaa tgaatacgaa gacaaagctg catattacct cgacggttta
    36601 ggcggcaaag aaaatatcaa agatgttact aactgtacaa caagattacg cttaactgtt
    36661 tatgatgaaa gtaaagttgc agatacggaa tatttcacgc atcaacaaat ggcacacgga
    36721 ttagtaaaga gtggtaagag tatccaagtt gtagtaggta tgacagtacc acaagttcgt
    36781 gaagcatttg aacaaatggt tgaggatcaa tcgtccgaag ataaataata caaaaaaaga
    36841 cacatcacta tacgcaatca ttgtaaatga tcatcgtata gatgtgtctt ttattatgat
    36901 atacgagctt tgctcataga tgacgatcta taattggcag atagaatgtc gatatttcta
    36961 gttgcaatcg tatgtaacaa tttagcaaat atttctggcg tacatataat atgtgcatga
    37021 gacttattca acgtataagt gatatcagtg tgattttgga acatattcat aatatcgatg
    37081 actttgtctt taatgaaagc atcttgcaag ccatcaatat tgatatcgaa acgctttgca
    37141 gattgatata aattatcaga atcaagtaat tcatttattt tctttggtga aagtacttgg
    37201 aaaatactat actgatgaaa acttaaaaat gaaatgaagt atggaagttg ttgctttggt
    37261 aagctaattt ctaatacata ctgattaaac tgtttgttta ttgagaattg caacgcatca
    37321 caaaattgta cacttttgca taacttactt aatttttttc tagtagtacg gtcatttaaa
    37381 ccggcgatgt tgatgatgta tgtttcgaat ttatcaatta acatgtgagg acctcccgag
    37441 gaatacatgg cattaaatac acgtttaata tttataaagg tgacttaatt ttgttcaagt
    37501 tgattttacc acgctttttt tctttattca ctaagacttt tgaatgaagt ttaaaataat
    37561 tgtttatcag tgataaaata tttgcaataa gaagagaatg actaaataat cttaattttc
    37621 agaaaagtaa ttgtaacctt actggtctta tggtaatatt tttcaatatt atcgacgagg
    37681 atgtgttaac aatgtttcaa aaattgagta tatttgccac gaagagtttt ttggtatgga
    37741 tgttagtagc agctgttatt ggatttattt tcccacaaca tgttgcgaca ttaggtaaat
    37801 gggtacctta tttacttggt attgttatgt taggtatggg attaacaatt acacctaatg
    37861 atttcaaaat ggtctttaaa gcacctagag cagtaattat tggtgtctgt ctacaattca
    37921 gtattatgcc cacattagca tttataattg caaagtcttt tcatttacca cctgatattg
    37981 ctgttggcgt aatattagtc ggatgttgtc cgggtgggac atcaagtaat gtaatgagtt
    38041 atttagccaa agctaacgta gcactttctg tttctattac gacggtctct acgttgctag
    38101 cgccattcgt tacacctgcg ttaatatatc tatttgcaaa tgaatggttg gaagtatctt
    38161 tcgtgagtat gttgtggtca gttgttcaag ttgtattaat tccaattgct ttaggtattg
    38221 ttttgcaaat tattaatcgt aaaattgctg aaaaagcttc tacagctttg ccaattatat
    38281 cagttgttgc tatttcatta attttagcaa tagttgtagg tggcagtaag caccaaatct
    38341 taactacagg attattaata tttttagtag ttattttaca taacgtatta gggtatacga
    38401 ttggatattg gttagctcgt cttttaaaat tagatcgaca agatcaaaaa gcagtcagta
    38461 ttgaagttgg aatgcagaac tctggtttag ctgtgtcatt agcagcattg cattttaatc
    38521 caattgcagc agtaccaggc gcagtgttta gtttcattca taatataaca gggcctattt
    38581 tagcaaagta ttggtcaaaa aagttataat tgcactaata gaatgaagtg gtcatcggac
    38641 tatgttaagc tttgataaag agaaaaaata gaggagtaaa tatatgtata gagcagttat
    38701 atttgatttc gatggaacaa taatagatac ggaacaacat ttatttaatg ttattaataa
    38761 acatttagag atgcataatg ccgatcctat aagcattgat ttttatcgtt cttctattgg
    38821 aggagcagct acagatttgc atgaccattt aattaaagcg attggttcgg aaaataaaga
    38881 taaactttat gaagaacatc atcttactag tacaacatta ccgatgattg atacgattaa
    38941 atcattgatg gcatttttaa agcaacgtca cattcctatg gcaattgcca caagtagtgt
    39001 gaaagcggaa ataatgccca cctttaaagc attaggtcta gacgattata tagaggtagt
    39061 tgttggtaga gaagatgttg aacaagttaa acctgaccct gaattatatt tatctgcagt
    39121 acaacaatta aattatatgc cgacacaatg tttggctatt gaagattctg taaatggtgc
    39181 aacagccgcg attgcagctg gattagatgt tattgttaat acgaataaaa tgacaagcgc
    39241 acaggacttt tctaatgtag attatgtagc aaaagatatt gattacgatc aaattgtagc
    39301 gcgtttcttt acgaaatagg aggcgtatca tgatgggtta cattatattg ttttttctag
    39361 ctggtccagt aattttaggc gttggaaatt tggtgattgg tcctatattt aacaaacaga
    39421 caccatttcg cgtgcaagta agatcttttg ttgttggttc aatgatttac ttaatactcg
    39481 caacaattgg ctatttttta ctattacaag gtaaacttta acgagaaaac caccttacct
    39541 cattaaatgg acgaccatat gtatgtgaaa tggtagaacg ttcatgttta tgtatgagat
    39601 agggtggttt aaatagttac atatatttta ataataacgt cacgatgata agtacaatta
    39661 agataatatc tatgcctacc ataattgtag ctcttgttgc attacttcct tgttcttttg
    39721 ctgatttcat agcacggtag tttggcacaa agctaataat tagtaagatt aatacaatta
    39781 caccaattaa tgctgttgtc atgatgaacg acctccttta tttttttcaa tcaattccca
    39841 aataaacgta gcaatcacac cgatgattaa cccagtgatg acaccgattt gtaccgttaa
    39901 aaatatggcg ccgaaaatga taccaataat taatgcaatc ggtaaagtcg taccgagttt
    39961 aatcttgcgc tctggagaat taataatagt aaatactgta agacaaatga gtatgaaagc
    40021 aagtgttgca ataatagttc ttccaactaa atataggatg tcaggttttt ccataccgat
    40081 ataatttatg atgaaaaatg ctacagcaaa gagtaccgat atttttgtag cacgtagcag
    40141 tatttgtttt aacattgata tactcctttt taatattatt aaaattatat cataattacc
    40201 aagaatagct gaagttgtat gtgactcaac ggtacttgag caactttttt aattttttag
    40261 aaaaatcaca aaataattgt ttgcaaagtt gcaaaagcct gctatagtag ttctgtaaac
    40321 gattgcatgg tatgcaaata ttaatgtacc aaaatcgata atttatagta taattacggc
    40381 aataagtttt tttatggatt tatttagtat caatcagaga tggggtaaga agttatggag
    40441 aacaatgaac tacaaagggg attgagtgcc cgtcaaattc aaatgattgc acttggtggt
    40501 acgattggcg tggggctttt catgggtgcg acaagtacaa ttaaatggac aggcccatca
    40561 gttatccttg catatttaat tgcgggtatc tttttatttt taatcatgag agcaatgggg
    40621 gaaatgattt atttaaaccc tacaacagga tcatttgcaa catttgcaag tgattatata
    40681 catcctgcag caggttatat gacagcatgg agtaatatat tccaatggat tgtagttggt
    40741 atgagtgagg tcatcgcagt aggagaatat atgaagtttt ggttcccgga attgccaact
    40801 tggattcctg gtgttattgc tattttatta ttaatggcag cgaatttatt ctcggtaaaa
    40861 gcgtttggag aatttgaatt ttggtttgct ttaattaaag ttgtaacaat tattttaatg
    40921 attattgctg gttttggtct tattttcttt ggttttggaa atggtggcca tgcggtaggt
    40981 atttctaatc tatggacaaa tggcggattt atgccaaatg gtattgttgg tttcttcttt
    41041 gcattatcaa ttgtaattgg ttcataccaa ggtgtggaac tgataggtat tacggcaggt
    41101 gaaactaaag atcctcagaa aaatatcgtt aaagcagtga atggtgttat ctggagaatt
    41161 ttaattttct atttaggcgc tatttttgtt attgtttcag tgtatccttg gaatcaatta
    41221 ggagacattg gaagtccgtt tgttgcaaca tttgctaaaa tcggtattac atttgcagct
    41281 ggattaatta actttgttgt attaactgca gcaatgtcag gatgtaactc aggtattttc
    41341 agtgcgagtc gtatgattta tacacttgct cataaaggtg aaatgccgaa aatatttact
    41401 aaaatcatga gaaatggtgt accattgtac actgttgtag cagtatctct tggtatttta
    41461 attggtgctt tattaaacgt aattttacct ttatatatcg atggagcgaa gagtattttc
    41521 gtatacgttt atagtgcgtc tattttacca ggtatgattc cttggtttat gattttattc
    41581 agtcatttac gttttagaaa attacaccct gaagaattgg aaggtcaccc attcaaaatg
    41641 cctggtggcg cagtaactaa ctatttaaca atcttattct taatattagt attggttggt
    41701 atggtattta atgttgaaac tagaatatca gtgcttattg gtgtcatctt cttaacgatt
    41761 gtaacgattt attattttat tagatataat aaaaataatg taaaagcaaa atagtatgat
    41821 tataatgtgc gcttatctta tgacaaactt ggccctagct tattaaagtt aaatagccaa
    41881 agaatgttat gagtaggcgc atttttttat aaatatagct taaatcgaag ttgtagtgtg
    41941 aaaaacttat aagtggcaat attatcgtta gtttttactg tgggctttaa aataaaaaat
    42001 agcattatta aaataacaaa cgcaattgat atatggaatg aatccgttaa tcaactgcgt
    42061 ttgtttttaa tataagaaat tgaatggcct tatcaaatca ttggtcatat gcgtatgtta
    42121 tacaagataa aagacatgta agctttgaaa atgatgcata aattattata tgacatgatt
    42181 aaagaattta tataagactg ttaatgttta aagttaatat ttgataaatg tttacgatag
    42241 ttatctaaag ccattttaga ttgtgttatg aaatctaatg atgcgtgata atttaatgca
    42301 acataacgat aatataaaat atcaatagtg aacatttgag caaataatga agttgttgct
    42361 cccatatgca tctcattttc atcagttttc ccataagtta aaacaatatt tgatgcctgt
    42421 gctacgggat tatcccttgt actagtaatt gtaattatag gtatatggta gtcatcaata
    42481 actttaacca ttgattgcat ttcactttgc gtaccattgt tagtaataag aataacactg
    42541 tcgttcgaat tgtgagttgc taataatgta gcaaaaatat gtgtttcttg aacaagttga
    42601 atattaagac ctattcttga taacttttgg tataagtcgg tagcaactac aaaagatgcg
    42661 ccaaaaccat atataaaaat cgtctcagaa cgttttaaac aatgacatat ttgatcaata
    42721 gttttatcat ttaattcgtt atttgcatga ttaagtgcgc gtgtagtacg tgtatggagt
    42781 ttagttctta aagattctgt gctttcgtta ttcattaatt caacattgta aattgatgat
    42841 gctttaggaa catatttaga tatatttatt ttcaagtcgt gaaaaccgcc atcagtaatt
    42901 ttcttgctga atcgaatgac tgatgatgta ctaatatcta ataagcttgc taagtcttga
    42961 gacctcattt taatcacctt ttgaggtgaa tttagtataa atttagctat ctttttttca
    43021 tttttagtca tatatggata ttgactatct atttctgtta gtacgtttga catattaatc
    43081 actccacttt aacgcaatat attttattag cgttggctat tgttgtttaa cgcgatggta
    43141 tttacaactt gtaacgtaag agaatgaaaa ataagctgaa tatgttgttt gtttaatgat
    43201 attgaattgt aaaactgtaa atttttttaa gatgtataga taaaaaatag catactccca
    43261 tgtgcaaata atatatcaat atattgatat attaccgtaa gtcatactgt atattcccaa
    43321 tttatacatt atgtactcat ctatttaaat cttagtaaat gtagttatat aagactaaat
    43381 ctaccatctt aatcattatg attataactc gattaatgca tctttacaat ttgaagaaac
    43441 tttttaattg
//
LOCUS       DANJFZ010000008        42884 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.008, whole genome shotgun sequence.
ACCESSION   DANJFZ010000008 DANJFZ010000000
VERSION     DANJFZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42884
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.008"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            108..221
                     /locus_tag="PWN45_000344"
     CDS             108..221
                     /locus_tag="PWN45_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622143.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            294..1283
                     /gene="ccpA"
                     /locus_tag="PWN45_000345"
     CDS             294..1283
                     /gene="ccpA"
                     /locus_tag="PWN45_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL0622144.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1381..2550)
                     /locus_tag="PWN45_000346"
     CDS             complement(1381..2550)
                     /locus_tag="PWN45_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDL0622145.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2575..3207)
                     /locus_tag="PWN45_000347"
     CDS             complement(2575..3207)
                     /locus_tag="PWN45_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0622146.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3376..5082
                     /gene="acsA"
                     /locus_tag="PWN45_000348"
     CDS             3376..5082
                     /gene="acsA"
                     /locus_tag="PWN45_000348"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDL0622147.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5522..7189
                     /locus_tag="PWN45_000349"
     CDS             5522..7189
                     /locus_tag="PWN45_000349"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL0622148.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7484..10171
                     /gene="harA"
                     /locus_tag="PWN45_000350"
     CDS             7484..10171
                     /gene="harA"
                     /locus_tag="PWN45_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDL0622149.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10302..10394
                     /locus_tag="PWN45_000351"
     CDS             10302..10394
                     /locus_tag="PWN45_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622150.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10374..11279)
                     /locus_tag="PWN45_000352"
     CDS             complement(10374..11279)
                     /locus_tag="PWN45_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0622151.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11661..12923
                     /locus_tag="PWN45_000353"
     CDS             11661..12923
                     /locus_tag="PWN45_000353"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL0622152.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(13017..14291)
                     /locus_tag="PWN45_000354"
     CDS             complement(13017..14291)
                     /locus_tag="PWN45_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL0622153.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14463..15080
                     /locus_tag="PWN45_000355"
     CDS             14463..15080
                     /locus_tag="PWN45_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL0622154.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15208..16674
                     /locus_tag="PWN45_000356"
     CDS             15208..16674
                     /locus_tag="PWN45_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL0622155.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16781..17911
                     /locus_tag="PWN45_000357"
     CDS             16781..17911
                     /locus_tag="PWN45_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0622156.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17872..17967)
                     /locus_tag="PWN45_000358"
     CDS             complement(17872..17967)
                     /locus_tag="PWN45_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622157.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(18061..19665)
                     /locus_tag="PWN45_000359"
     CDS             complement(18061..19665)
                     /locus_tag="PWN45_000359"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL0622158.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19652..20812)
                     /locus_tag="PWN45_000360"
     CDS             complement(19652..20812)
                     /locus_tag="PWN45_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL0622159.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20926..21372)
                     /locus_tag="PWN45_000361"
     CDS             complement(20926..21372)
                     /locus_tag="PWN45_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDL0622160.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21452..22195
                     /locus_tag="PWN45_000362"
     CDS             21452..22195
                     /locus_tag="PWN45_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0622161.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22411..23013)
                     /gene="rpsD"
                     /locus_tag="PWN45_000363"
     CDS             complement(22411..23013)
                     /gene="rpsD"
                     /locus_tag="PWN45_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL0622162.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23257..23721)
                     /locus_tag="PWN45_000364"
     CDS             complement(23257..23721)
                     /locus_tag="PWN45_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL0622163.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            23858..25552
                     /gene="ezrA"
                     /locus_tag="PWN45_000365"
     CDS             23858..25552
                     /gene="ezrA"
                     /locus_tag="PWN45_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL0622164.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(25626..25766)
                     /locus_tag="PWN45_000366"
     CDS             complement(25626..25766)
                     /locus_tag="PWN45_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622165.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            25915..27063
                     /locus_tag="PWN45_000367"
     CDS             25915..27063
                     /locus_tag="PWN45_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0622166.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            27063..28286
                     /gene="thiI"
                     /locus_tag="PWN45_000368"
     CDS             27063..28286
                     /gene="thiI"
                     /locus_tag="PWN45_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL0622167.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            28330..29100
                     /locus_tag="PWN45_000369"
     CDS             28330..29100
                     /locus_tag="PWN45_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL0622168.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            29198..29692
                     /gene="tpx"
                     /locus_tag="PWN45_000370"
     CDS             29198..29692
                     /gene="tpx"
                     /locus_tag="PWN45_000370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL0622169.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            29815..30762
                     /locus_tag="PWN45_000371"
     CDS             29815..30762
                     /locus_tag="PWN45_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0622170.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            30850..32052
                     /locus_tag="PWN45_000372"
     CDS             30850..32052
                     /locus_tag="PWN45_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL0622171.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(32298..32798)
                     /locus_tag="PWN45_000373"
     CDS             complement(32298..32798)
                     /locus_tag="PWN45_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0622172.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            32939..34057
                     /gene="ald"
                     /locus_tag="PWN45_000374"
     CDS             32939..34057
                     /gene="ald"
                     /locus_tag="PWN45_000374"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDL0622173.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            34181..34279
                     /locus_tag="PWN45_000375"
     CDS             34181..34279
                     /locus_tag="PWN45_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622174.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(34373..35428)
                     /locus_tag="PWN45_000376"
     CDS             complement(34373..35428)
                     /locus_tag="PWN45_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0622175.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            35603..36292
                     /locus_tag="PWN45_000377"
     CDS             35603..36292
                     /locus_tag="PWN45_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0622176.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(36597..37010)
                     /locus_tag="PWN45_000378"
     CDS             complement(36597..37010)
                     /locus_tag="PWN45_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0622177.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            37215..38513
                     /locus_tag="PWN45_000379"
     CDS             37215..38513
                     /locus_tag="PWN45_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0622178.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            38774..39715
                     /gene="pde2"
                     /locus_tag="PWN45_000380"
     CDS             38774..39715
                     /gene="pde2"
                     /locus_tag="PWN45_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDL0622179.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            39736..>42884
                     /locus_tag="PWN45_000381"
     CDS             39736..>42884
                     /locus_tag="PWN45_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL0622180.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSM"
BASE COUNT        14868 a         6233 c         7856 g        13927 t
ORIGIN      
        1 tatctgtaaa atataataga ttcacacatt tgttgctgaa atgtgaacat ttttcaacaa
       61 atgcaattga tatttgaaaa ggctttctca aaacattaca attaaaaatg aaaaaagttt
      121 atataaaatt aaaatatatc gttcgttatc atttagcgtt tgtttttatt tcaagctttt
      181 cgctaaattt ttccaaacaa aaatatgtta ctgtaaatta aaatatggta aactatgaaa
      241 atgaaatgaa aacatgttat tataatgaat aaaacgttta caaggaggaa attatgacag
      301 ttactatata tgatgtagca agagaagcgc gtgtctctat ggccacagtg tcgcgtgttg
      361 ttaatgggaa ccaaaatgtt aaagcagaaa ctaaaaataa agttaacgaa gtcattaagc
      421 gtttgaatta tcgtccaaat gctgttgcta gaggtttagc tagtaaaaag acaacaacag
      481 taggtgtgat cattccagat atatctaata tctattattc acaacttgct cgtggacttg
      541 aagatattgc aacaatgtat aaatatcact caattatttc aaattcagat aacgatcctg
      601 aaaaggaaaa agaaattttt aataacttat taagtaaaca ggttgatggt attattttcc
      661 ttggtggtac aattactgaa gaaatgaaag aattgataaa tcaatcatct gtacctgtag
      721 tagtatcagg aacaaatggt aaggatgcac atatagcatc agttaatatt gattttactg
      781 aagctgcgaa agaaattacg ggagaattaa ttgaaaaagg cgctaaatca tttgctttag
      841 taggtggaga acattctaaa aaagctcaag aagatgtttt agaaggttta actgaagtgt
      901 taaataaaaa tggccttcaa ttaggtgata cattgaattg ttctggtgct gaaagttata
      961 aagaaggcgt aaaagctttt gccaaaatga aaggcaattt gccagatgcc attttatgta
     1021 tcagcgacga agaagcaatt ggtattatgc atagtgcaat ggatgctggt attaaagttc
     1081 cagaggaatt acaaattatt agtttcaata atacacgatt agttgagatg gttagaccac
     1141 aactttctag tgttattcaa ccattatatg atatcggtgc agtagggatg cgcttattaa
     1201 caaaatatat gaacgatgaa aagatagaag aaccaaatgt agttttacct cacagaattg
     1261 aataccgagg aactacaaaa taaattcaca aaattaggca ttcatctaac gacccaaatt
     1321 atatgggtgt tggaagaatg ccttttattt atcttttaaa atcgttgcag attaggttac
     1381 ttattgacga gtagattcgt accaactcgc tatatgtaaa gctaattttt tatttttttc
     1441 actaatttct tttgtgcggg ggacataggt ataatcattt aaacgatctt cccatctttt
     1501 aggtaataat tcagatgaat aatgtttcca tttattaatc cattctaacg gtaaataacc
     1561 actttgaatt ggttgatcaa ttaaacttaa gaatacatga ctccatgcac gtggtacgac
     1621 tctccaaata ttgtagcctc cgccaccaaa cataattacc tttccattcg tataagaatc
     1681 agctaaatat tttacaaaat atggaatttc atataatgaa tgtaacgtac aatttagatg
     1741 agttagtgga tcacgataat gtatatcgac accatttacg cttagaataa tatcaggttt
     1801 aaaactcttt acgacaggct caactgttaa tttaaaacac tccaaaaatg atgcatcttc
     1861 tgtatacggt tcaagtggga catttacagt gtgtccatag ccgatatctt caccgcgctc
     1921 agtatagtga ccagagcctg ggaaaagaaa ttttccggtt tcatggatag aataagtagt
     1981 aacatggtta tcggcataga aactccattg tgtaccatct ccatgatgtg catcggtatc
     2041 tatgattaaa acgcgttgat tgtattcttt agctaagtat tgtgcggtaa ttgcaatatc
     2101 attgtatata caaaaaccac ttgctcgacc aggttgagcg tgatgcaaac caccacctaa
     2161 gtgacaacca tttaatactt tgcctgacat aataagatct gctaaagtta aagcgcctcc
     2221 aacaattgtg gcactatggc ggtgcatatg cttaaattga ccattctctt catcatttaa
     2281 tccatatttc ttagcctcat cttcactgat aatgccatgt gaagcatgct taatagcttc
     2341 gacgtaatca tatttatgaa ttaacattaa ttcgtcatct gttgcaattc taggttgtac
     2401 tatttgttct ggagacaata aatttgcatt caaaagtagc tctgttgtta attttaaacg
     2461 catttgattg aagggatgtt ggtcatgaaa tcgatattgt aataacttat ctgaataaac
     2521 atatgcagtt tttgatgaat gttgttgcat ataatccctc cgatattcca aaaattaaaa
     2581 gaaaaaccga ttcatataac gaatatcatc aaacgcttgt tgctgttcta atgtaatgtt
     2641 tttgccaatt cttgccatta aacaattagc tggatgactt gttatttctg gatcatctgt
     2701 agcgaatatt tcaagtccac cagttgccat taaccgctgc attaattttt tatagtcaaa
     2761 tacatctaac tttgaatttt ttaaatccca atgccagtaa tattctgtag ttataacgat
     2821 ataattctcg aattctggtg tagaaaggct aagttgtatc agcttttctg caagttgtaa
     2881 ttgcctaaaa ttgatgctga cttcaattgc ccccaattcg attaaatatg gaagcttacc
     2941 tgtagaccag cgctcaattt catcaggata gtggaaagtg acataaccca caatgagttg
     3001 atcttggcga gcaacataaa ttctaccttc tggtaatgtt gtaatttcta acaaagcttt
     3061 atactgatct tcagcatctc taaatgcggt taaatgcgca tcgaaagtaa gcgctttcaa
     3121 atcttcgtgt gttaaaggac cttcaataac aaattgcttg tcatgaatgt aataatcttc
     3181 ggattgatac gtctttaaat gattcatatt ttcaactcct caatcgacgt cgtgattgta
     3241 ttaaattcat tatatagaaa atttacaata attaatacta gaaaaaggat aaaagtaaaa
     3301 attttgaata attagaaatg ttatgtataa tattgagaaa gaaagcgttt tcacataaca
     3361 aagggggagt ttcaaatgaa agtcgaagtt tataaaggag cgcaaggtaa acataacctt
     3421 aaagattatg aagaaacata taatactttt gattggaaag acgtagaaca agcattttct
     3481 tggagtgaaa ctggaaaaat gaacatggca tatgaatgca tagatcgcca tgtagatcaa
     3541 ggattagggg ataaaatagc gttaaattac aaagatgagc acagaaaaga atcgtatact
     3601 tataaagata tgcaacggtt atctaataaa gcagcgaatg ttttgtctga acatgcagaa
     3661 gttgacaaag gtgacagagt atttatattt atgtcgcgta cacctgaact atattttgcg
     3721 ttgttaggtg ttttaaaaat tggtgcaatt gttgggccgt tatttgaagc atttatggaa
     3781 aaggcagttg cggatagatt agagaacagt gaagctaaag tgttaattac taataaggca
     3841 ttgttacctc gagtacctgt agataaatta ccaaacttga aaaaaattgt tgtcgtagat
     3901 gaggatgtag aagacaatta catagacttc attagtttga tggaaactgc tagcgatgaa
     3961 tttgacattg aatggttaaa gtcggatgat ggtttgattt tacattatac atcaggttct
     4021 actgggcaac ctaaaggtgt attgcatgtt caacaagcaa tgttagtgca ctatatttct
     4081 ggaaaatatg tattagattt acaagaagat gatgtttatt ggtgtacagc agatccaggt
     4141 tgggttacag gaacatctta tggtattttt gcaccatggt taaatggcgc tacaaattgt
     4201 atagctggtg gtcgcttttc gccagaacag tggtatagta tgattgaaga ttttaaagtg
     4261 acgatttggt atacggcacc aacagcttta agaatgttaa tgagtgctgg tgacgatatt
     4321 gttgagaaat atgacttgtc atcgttacgt tcgattctat cagtaggtga gcctttaaat
     4381 cctgaagtta taaaatgggc gaaaaaagta tacggtttaa cggtgttaga tacttggtgg
     4441 atgacagaaa caggtggaca tatgattgtt aactatccaa cgatggacgt caagcttggc
     4501 tcaatgggca aaccattacc tggtattcaa gctgcaatta tcgatgatgc agggaatgaa
     4561 ttaccaccaa atcgaatggg caaccttgct ataaaaaaag gctggccatc aatgatgtat
     4621 cgtatctgga agaatccaga aaaatataaa tcatatttta ttggagactg gtatgtatct
     4681 ggtgattcgg catataaaga tgaagatggt tacttctggt tccaaggacg tgttgatgat
     4741 gtaattatga cagctggtga acgagttgga ccatttgagg ttgagtctaa attggttgaa
     4801 cacgaagcag ttgccgaagc aggaattatt ggtaaacctg atccggttcg cggtgaaata
     4861 attaaggcgt ttgttgcact gagaaaagga tatgaaccaa cagacgaatt aaaagaagaa
     4921 attcgtatat ttgttaaaga aggtttgtcg gcacatgcag caccacgtga aatcgaattt
     4981 aaagataaat tacctaaaac acggtcaggt aaaattatga gacgtgtatt aaaagcttgg
     5041 gaattaaatt tagatgctgg ggatttaagt acaatggaat aatgacatga atgttattga
     5101 agattttttt cgaagaataa agggtgacaa catatttcat gtcaatgttt aaataatcgt
     5161 ttactttacg ataagcaata taaagaactg ttaacttgtg tcatatcatt tcgtagaaag
     5221 catttgaaaa tgatgacata acaataatgg catatcttta tattgctttt tatttttaat
     5281 atgatctttg gaagatgatt attttaaata atagaaaaat atagttatca atagtatcaa
     5341 gcgctaaaag ttgtataata caaaacttta ataagtgaat ttattgcaaa aatgaaagcg
     5401 ctaacccgat ttagtcgaca agtttttaac agttcgttat tatatgaatg taagtaaaaa
     5461 tttcttagct acaacttaca tattataaat gcataaatta aacaaaaagg ggcgaaaaaa
     5521 gttgactcat ttatcagatt tagatattgc gaatcaatca acactacaac caattaagga
     5581 tattgctgca tcagtaggta tttcagagga tgcattagaa ccttatggtc attacaaagc
     5641 taaaatcgac attaataaaa ttacgccaag agaaaacaaa gggaaagttg ttttagtaac
     5701 tgcgatgagc ccaacaccag ctggtgaagg taaatcaacg gttacagttg gtttagctga
     5761 tgcattccat gagttaaata aaaacgttat ggttgcatta agagagcctg ctttaggacc
     5821 aacatttggt atcaaaggtg gtgcgactgg tggtggttat gcgcaagtct tacctatgga
     5881 agatatcaac ttacatttca acggagattt ccatgcgatt acaactgcaa ataatgcatt
     5941 gtctgcgttt atcgataatc atattcacca aggtaacgaa ttaggaatcg atcaaagacg
     6001 tattgagtgg aaacgtgtat tagatatgaa tgatcgtgca cttagacatg taaacgttgg
     6061 gttaggtgga cctacaaatg gtgtaccacg tgaagatggc tttaatatta cagtagcgtc
     6121 tgaaattatg gcgattttat gtttaagtag aagtattaaa gacttaaaag ataaaattag
     6181 tcgtattact attggttaca ctagagatcg caagccagtt acagttgcag atttaaaagt
     6241 ggaaggtgca cttgcaatga ttttaaaaga tgcaataaaa ccaaacttag tacaatcaat
     6301 tgaagggaca cctgcattag ttcatggtgg accatttgcg aatatcgcac acggttgtaa
     6361 ctcaatttta gcaactgaaa cagcacgtga tttagctgat atcgttgtaa cggaagctgg
     6421 atttggttca gacttaggcg ctgaaaaatt catggacatt aaagtgcgtg aagcaggatt
     6481 tgatccggca gctgtcgttg ttgttgcgac aattcgtgcg ttaaaaatgc atggtggtgt
     6541 agcgaaagat aatttaaaag aagaaaatgt agaagcagta aaagcaggaa ttgttaattt
     6601 agagcgtcat gttaataata ttaaaaaatt cggtgtagaa ccggttgttg caattaatgc
     6661 atttatacat gataccgatg cagaagtaga atatgtaaaa tcttgggcta aagaaaataa
     6721 cgtacgaatt gccttaactg aagtttggaa aaaaggtggt aaaggtggcg ttgacttagc
     6781 aaatgaagta ttagaagtca ttgatcaacc taattcattt aaacctttat atgaattaga
     6841 attaccatta gagcaaaaga ttgaaaagat tgtgactgaa atctatggcg gttcaaaagt
     6901 aacgtttagc agtaaagcgc aaaaacaatt aaaacaattt aaagaaaatg gttgggataa
     6961 ttacccagta tgtatggcga aaacacaata ttcattctca gatgatcaaa cgttgttagg
     7021 tgcaccatca ggatttgaaa ttacaattcg tgaattagaa gcgaaaacag gtgcaggatt
     7081 tatcgtagcg ttgacaggtg caatcatgac tatgcctggt ttacctaaaa aaccagcagc
     7141 attaaacatg gatgttactg atgatggtca tgcaattggg ttattctaat aaatcatgtc
     7201 aattgtttaa taaagataag taaatagttt aatagaccgg actgttggag atgcattatt
     7261 tcagcagttc ggttttttgc tgtgctaaaa atagattcaa tttggcgaat ctaacgacaa
     7321 tgtttgaagg tggttaatta atgtatatga agataaaaag tgggcttgaa gaataggaaa
     7381 gcgatgcaat gaatattcca tattaaaaaa aataataaaa taggttgcaa tatttaattg
     7441 ggatgcgcta caattaacac taataatagg agttgttaac aacatgaaca aacatcaccc
     7501 aaaattaagg tctttctatt ctattagaaa atcaactcta ggcgttgcat cggtcattgt
     7561 cagtacacta tttttaatta cttctcaaca tcaagcacaa gcagcagaaa atacaaatac
     7621 ttcagataaa atctcggaaa atcaaaataa taatgcaact acaactcagc cacctaagga
     7681 tacaaatcaa acacaacctg ctacgcaacc agcaaacact gcgaaaaact atcctgcagc
     7741 ggatgaatca cttaaagatg caattaaaga tcctgcatta gaaaataaag aacatgatat
     7801 aggtccaaga gaacaagtca atttccagtt attagataaa aacaatgaaa cgcagtacta
     7861 tcactttttc agcatcaaag atccagcaga tgtgtattac actaaaaaga aagcagaagt
     7921 tgaattagac atcaatactg cttcaacatg gaagaagttt gaagtctatg aaaacaatca
     7981 aaaattgcca gtgagacttg tatcatatag tcctgtacca gaagaccatg cctatattcg
     8041 tttcccagtt tcagatggca cacaagaatt gaaaattgtt tcttcgactc aaattgatga
     8101 tggagaagaa acaaattatg attatactaa attagtattt gctaaaccta tttataacga
     8161 tccttcactt gtaaaatcag atacaaatga tgcagtagta acgaatgatc aatcaagttc
     8221 agtcgcaagt aatcaaacaa acacgaatac atctaatcaa aatacatcaa cgatcaacaa
     8281 tgctaataat caaccgcagg caacgaccaa tatgagtcaa cctgcacaac caaaatcgtc
     8341 aacgaatgca gatcaagcgt caagccaacc agctcatgaa acaaattcta atggtaatac
     8401 taacgataaa acgaatgagt caagtaatca gtcggatgtt aatcaacagt atccaccagc
     8461 agatgaatca ctacaagatg caattaaaaa cccggctatc atcgataaag aacatacagc
     8521 tgataattgg cgaccaattg attttcaaat gaaaaatgat aaaggtgaaa gacagttcta
     8581 tcattatgct agtactgttg aaccagcaac tgtcattttt acaaaaacag gaccaataat
     8641 tgaattaggt ttaaagacag cttcaacatg gaagaaattt gaagtttatg aaggtgacaa
     8701 aaagttacca gtcgaattag tatcatatga ttctgataaa gattatgcct atattcgttt
     8761 cccagtatct aatggtacga gagaagttaa aattgtgtca tctattgaat atggtgagaa
     8821 catccatgaa gactatgatt atacgctaat ggtctttgca cagcctatta ctaataaccc
     8881 agacgactat gtggatgaag aaacatacaa tttacaaaaa ttattagctc cgtatcacaa
     8941 agctaaaacg ttagaaagac aagtttatga attagaaaaa ttacaagaga aattgccaga
     9001 aaaatataag gcggaatata aaaagaaatt agatcaaact agagtagagt tagctgatca
     9061 agttaaatca gcagtgacgg aatttgaaaa tgttacacct acaaatgatc aattaacaga
     9121 tttacaagaa gcgcattttg ttgtttttga aagtgaagaa aatagtgagt cagttatgga
     9181 cggctttgtt gaacatccat tctatacagc aactttaaat ggtcaaaaat atgtagtgat
     9241 gaaaacaaag gatgacagtt actggaaaga tttaattgta gaaggtaaac gtgtcactac
     9301 tgtttctaaa gatcctaaaa ataattctag aacgctgatt ttcccatata tacctgacaa
     9361 agcagtttac aatgcgattg ttaaagtcgt tgtggcaaac attggttatg aaggtcaata
     9421 tcatgtcaga attataaatc aggatatcaa tacaaaagat gatgatacat cacaaaataa
     9481 cacgagtgaa ccgctaaatg tacaaacagg acaagaaggt aaggttgctg atacagatgt
     9541 agctgaaaat agcagcactg caacaaatcc taaagatgcg tctgataaag cagatgtgat
     9601 agaaccagag tctgacgtgg ttaaagatgc tgataataat attgataaag atgtgcaaca
     9661 tgatgttgat catttatccg atatgtcgga taataatcac ttcgataaat atgatttaaa
     9721 agaaatggat actcaaattg ccaaagatac tgatagaaat gtggataaag atgccgataa
     9781 tagcgttggt atgtcatcta atgtcgatac tgataaagac tctaataaaa ataaagacaa
     9841 agtcatacag ctgaatcata ttgccgataa aaataatcat actggaaaag cagcaaagct
     9901 tgacgtagtg aaacaaaatt ataataatac agacaaagtt actgacaaaa aaacaactga
     9961 acatctgccg agtgatattc ataaaactgt agataaaaca gtgaaaacaa aagaaaaagc
    10021 cggcacacca tcgaaagaaa acaaacttag tcaatctaaa atgctaccaa aaactggaga
    10081 aacaacttca agccaatcat ggtggggctt atatgcgtta ttaggtatgt tagctttatt
    10141 cattcctaaa ttcagaaaag aatctaaata attaactaaa tatagcatat gtatgattaa
    10201 ctttgtagac aatgtgaaag caattaattt ataaactatt gattggttta atggctttcc
    10261 tttagagtaa ataaaaagaa cagcagtgag aaattttcta attgaaaata atcttactgc
    10321 tgtttttaat atttggattc attgttgtgg ttactttaaa aagtgagcat caattaacgc
    10381 ttttttcgat ttaacaaatg tgatttaata tcatatttta atgcgtcgtt gtattctttt
    10441 tcagtgattt gatcttcgat taacatacgc tttaatacat aatgttgtct ttgaatacta
    10501 tatttcaaat ctttatccga ttttaacgtt ccatcttttt cgtagggtgt atagccatat
    10561 gggctttgca acaaaccgat aaggtatgca gattgtgcaa ttgataaatc ttttggtgga
    10621 ataccaaata gactatatga agcggatgca attccggaaa tattagcgcc attataatct
    10681 ctaccgaagg gaactatatt taaatatgta tatataattt catcttttga gagtaggtgt
    10741 tctaatctaa ttgctaggcg aagttcattt gcttttctac tatatgtttt ttcgttggta
    10801 agaacttgat ttttaacaag ttgttgtgta attgtgctac cacctgaact ttgatcagta
    10861 ttaaaaatat cttgtatcat tgctcttaaa atcgcctttg gtaagatgcc atcatgttta
    10921 taaaataaag tgtcttcaga tgacgttaat gctttaatga catttggact tgatgtttta
    10981 gggcctataa tgagtgagtt ttgagaatgg ttatactcat ataataaatt tttgttatta
    11041 tgatctaata attcatcgcc aggtatttgt cgaacttttt ttattaaagc atcatctgat
    11101 aatgagtcgg acgttttagt taaatgatga aaatataaag acatcgcaat cacagcgata
    11161 gcaacgatgg caatgaaaat cataaatata aaaacaatga catgtttgat agctttatat
    11221 atcttttcaa aatgatatat acgatgattc aattgatgat tgttgtcttg attcgtcatc
    11281 tttaacctcc taaattacgt atagtatacc atatttaagc aatttgactt ttattttaac
    11341 gttaattata attagggaaa atgcataaag aataggataa aaggacaagt aatattattt
    11401 gaaatcatca gagagctagt ggtcgctgtg aactagtgtt tttaataata tgaatacacc
    11461 tttataacta acatgtccta cgtttattaa tgaacgtatc attaatagag tgaacattat
    11521 cttatgatta tgtttaagtt tggtggtaac gtgcaatcgc gcccttacat atttgttgta
    11581 agggtttttt ttacgttcaa aaaactttaa agtattttaa caagtattca ataaataatt
    11641 ataaatagga ggaaataata atgacgaatg tattaattga agatttaaaa tggagaggtc
    11701 ttatttatca acaaactgat gaacaaggta ttgaagattt attaaataaa gaacaagtga
    11761 cgttatactg cggtgccgat ccaacggcag atagtttaca tattggtcac ttactaccat
    11821 tcttaacatt aagacgtttt caagaacatg gacatcgtcc tatcgtttta attggcggtg
    11881 gtacaggtat gattggtgat ccatcaggta aatcagaaga acgtgtgcta caaacagaag
    11941 aacaagtaga taaaaatatc gaaggtatta gtaagcaaat gcacaatatt tttgaatttg
    12001 gaacagacca tggtgcagtg cttgttaata atagagactg gttaggacaa atctcattaa
    12061 ttagtttttt acgtgactat ggtaaacacg tcggcgttaa ttacatgtta ggtaaagatt
    12121 caatccaaag tcgtttagaa catggtattt catatacaga attcacatac acgattttac
    12181 aagctattga tttcggtcat ttgaatagag aattgaattg taagattcaa gtaggtggat
    12241 cagatcaatg gggtaatatc acaagtggta ttgaattaat gcgtcgtatg tatggtcaaa
    12301 cagacgcata cggtttaact attccgcttg taactaaatc agatggtaag aaatttggta
    12361 agtctgagtc aggtgctgtt tggttagatg ctgaaaaaac aagtccttat gaattttatc
    12421 aattctggat taatcaatca gacgaagatg taattaaatt cttaaaatac tttactttct
    12481 taggaaaaga agaaattgat cgcttagaac aatctaaaaa tgaagcaccg catttacgtg
    12541 aagctcaaaa aacattagct gaagaagtaa ctaaatttat tcatggtgaa gatgcattaa
    12601 atgatgcaat ccgtatttca caagcattat ttagtggtga tttaaaatca ttatcagcga
    12661 aagaattaaa agatggattt aaagatgtgc ctcaagtgac attatcaaat gacacaacaa
    12721 atatcgttga agtccttatt gaaacaggca tttctccttc taaacgacaa gcacgtgaag
    12781 atgttaacaa tggtgcgatt tatattaatg gtgagagaca acaagatgtt aattatgctt
    12841 tagcaccaga agataaaatt gatggcgaat ttacgattat tcgtcgcggt aagaaaaaat
    12901 acttcatggt taactatcaa taaatataat tgcatagcta aataaattag agcctactca
    12961 tattcattcc taagaatgta atgagtaggc tcttaatgta cttttctgtc tgtaaattat
    13021 ctaaagaaat ctctatcgcc ttggccaggt gattgacgtt ctgatcggct ttgacgtttt
    13081 ggttgttctt tttgttgttt taatttcact ttaacttctt ttgttttacc atcacggata
    13141 acggtaacag tgactgattc accaggtttt ttattttcat ataaatagct tcttaaatca
    13201 acatcatctt taattttctt gccatcaatt tctgtaataa tatcaccttt tttaagatca
    13261 atatcactat cagctttggc gacataaata ccgtcttctc tatcagtatg aagttgctcg
    13321 cgctcttctt caggaatatc tttcaaatta attaaaccaa taccaatcga agggcggtca
    13381 attttaccat gttttacaag ttgttcaatt gttactttaa cttcattact tggaatagca
    13441 aacccgatac cttcaacttg tgtcgcagca attttcattg agttaatacc aactaaatta
    13501 ccattaatat ctactaatgc gccacctgag ttacctgggt taatagcagc atctgtttga
    13561 agaacgctaa cttttgtatt gccaccagtt gtctcagcgt caatcgtacg ttcgcttgct
    13621 gaaatgatac cagatgttac agagttagca aattgtaatc ctaatgggtt acccattgcg
    13681 aatacgctat cgccagtttg tacttttgaa gagttggcaa attgaatcgc tttaatacct
    13741 tttgtatttt caattttaag tacagcaata tcagttactg catctttacc aactaatttc
    13801 gctttaactt gttttttatt atgtaattgg actctaattt catttgcgcc atcaataaca
    13861 tgattgtttg taacgatata agctgaattg ttgtttattt gatagataac acctgaacct
    13921 actccagctt cagatggttt agatgatttg ccttttaata agtcgtctac acttgatgct
    13981 ttttgcatgt taataactcc aacaattgta ggggagacag attttatcat ttcatgaacg
    14041 gtaccgaatt tcttgctttg accgtctaat tgattgccac ctttattatt tgttgtctga
    14101 acagttgaac catctttatt taaaattgta ctatttaata ctttgcctat accaagtact
    14161 agaagtgcac caataattcc agcaatcaat gcaacgatga ctgttttaaa ccatggaaat
    14221 ttaggtcttc tgtatctagg tgtttggcta tggtttgttg tagaatgatc tgtatgatta
    14281 aaatctgaca tacttaacct ccattatatg atttatatat gctttaatta tgtctttttt
    14341 ttataattat tgctagtaat acactataca actttagatg tattttcaga ctatcttgtt
    14401 ctagtaaaaa tatagtgata aaatctagtt gttataaaca aataagttaa gtaggtggca
    14461 caatgtattc agtgattagt aagattttga atttcatttt agttaaaatg tcaaaatcat
    14521 tatacgtaat cggtaaagat aatattccta aggatagtaa atatgtcgta acttgtacgc
    14581 atgaaagtta taacgaagtc attatgctag gtatggcatt atatcctaat caaattcatt
    14641 atatggctaa aaaagaatta ttcaaaaata aatggattgg taaattttta acgtctttaa
    14701 atgcatttcc tgtagatcgt gaaaatccag gacctagtac tttaaaacgc ccaatcaatt
    14761 tgttgaaaga taataagaca gttggtatct tcccaacagg ttctcgcaca tcgcaagaag
    14821 gtgcaccttt gaaacgtggt gcttcaacaa ttgcaatgtt aagtaagtca ccaatattac
    14881 ctgttgctta tgttggtcct actaagattc acggtctttt aacaggacaa gcatacataa
    14941 atattggcaa accaatcgat attaacgatt tacctaaaga tttaaaacga aacgagcgta
    15001 tcgattatat tacaaaagag attgaaacac gaacagcaaa gttacaacaa gaattgcatg
    15061 aaattgtaaa aagtttataa aataacttga ggtagaaata tagctggtta gtaagcctag
    15121 gtgactataa cggagtatcg aagtagttat tctagttaca ttagtaatct ttatatttct
    15181 acattatttt aagaaggatg acattcaatg tataaatttt tccaaaattt agggcgttcg
    15241 ttaatgttac cagttgccat tttaccggct gcagcaatta ttgccggaat tggtaatacg
    15301 ttaaatgcac ttcatgcgac gccgaaaatc gcaatgttct ttactacggt aggtactacg
    15361 attttagagc aattaggtat tttatttgca attggtgtag ctataggaat ggcaaagaaa
    15421 aatgatggtg tagtcgcgct tgcagctacg ttaggatatt ttttagttac agttgtatta
    15481 tcaccaatga aattagcgcc tttattagga atgaaagctt cggaaataaa ttcagctttt
    15541 gaaaagatga ataatggcaa tgtctttgtt ggtattgtaa taggtttaat agcagcttat
    15601 gcatacaata agtttagtga aacagaatta ccattagcat tatcattttt tagtggtaaa
    15661 cgtctagttc caattatgac tgcattttac tgtacatttt tagttgtcat attgttattc
    15721 ttatggccac tactttattc atggattgta aaatttggtg aatctatagt cggtttagga
    15781 tcgtttggag ctttcatata tggtgttgcc aatagattat taattcctac aggattacat
    15841 catgcactga atagtgtgtt ctggtttgat acgattggta ttaatgatat tggtaaattc
    15901 caaagtggca aagatgctat aaaaggtatt acaggacgct atcaagcggg attcttccca
    15961 atcatgatgt tcggtattcc agcggctgcg ttagcaatgt atcatacagc taaaactact
    16021 cagaaaaaac aagtttacgg ttggttccta gcgagttcag ttgcagcatt tttcgttggt
    16081 gttacagagc cgattgagtt tgcatttatg tttgttgcgc caatcttata tgttgttcat
    16141 gcgttattaa caggattatc tttatttatc gcagctacat tccattggac agctggattt
    16201 tcatttagtg caggtttgat agattatgtg ttatcactaa ttaatccagt atcaaatcat
    16261 ccattgatgt tgcttgttca aggtgttgta tttttcatct tatactatgt gattttccgt
    16321 gtagtaatcc aagtatttaa cttgaatacg attggtagag gtgaaaatga attagttgac
    16381 ccaacagttg taaaagataa tattgctcct ggtgaaaatg atattaaaca aagtaaatat
    16441 catcaacatg ctatacaaat attagaaggt ttaggcggtc aagagaatat tgttaattta
    16501 accaattgtg caacaaggtt gcgtctagag ttaaaagaca catctattat tgataaacag
    16561 aagattaaaa acgccggtgc agttggtgtt actgttaatg gcaaacattc aactcaagtt
    16621 attgtcggta cacacgtaca gcaggttgct gatgaaattg aaaaacattt ataaaattta
    16681 aacagctaaa gcgcaaatct aacttcaata gggtttgcgc tttttttagt caaatagtag
    16741 aatgatttat atctgaattt tcagaaatgg agtggggatt gtgaagaaaa ttttatttga
    16801 tgttgatggc gtttttttaa gtgaagaacg ttgtttcgat gtctctgcat taacggtata
    16861 tgaattatta atggataagt gttatttagg tcttcattca catatagatt gggaaacttt
    16921 gactgataac gatatacaag acattaggaa tcgaattttt caaaaagata agattttaaa
    16981 caaattaaaa tcgctagggc tgaattcaaa ttgggatatg ttatttatcg tttttagtat
    17041 tcatttaatt gatattttga aaaagttatc acatgatgaa attgaggcat tcatgtatca
    17101 agatgagcct gtcgaattga agttgcaaaa tataagcaca aacttagcag actgttttaa
    17161 tttaaatgaa caactacctt tacaattttt ggataatgta aaagttggta aaaataatat
    17221 ttatgctgct ttggaagagt ttgcaacaac agagttacat gtttcggatg ctactttatt
    17281 tagtttgaag ggtgcattat ggacgttagc gcaagaagtt tatcaagaat ggtatttagg
    17341 atcgaagttg tatgaagatg ttgaaaagaa aatagcacga actactttta agacaggtta
    17401 tatttatcaa gaaattattt tgagaccagt agatgaagtt aaggtacttc tgaatgattt
    17461 aaaaggtgct ggtttcgaat taggtattgc aacaggtcgt ccttatactg agactgttgt
    17521 gccatttgaa aatttaggat tgttaccata ttttgaagct gattttattg caacagcaag
    17581 tgatgtttta gaagcagaga atatgtatcc gcaagcacga ccattaggaa agccgaatcc
    17641 ttttagttat atcgcagctt tatatggtaa taatcgcgat aaatatgaat cttatatcaa
    17701 taagcaagat aacattgtaa ataaagatga cgtatttata gtaggcgatt cgttagctga
    17761 cttattaagt gctcaaaaaa taggtgcaac gtttattgga acattaacag gtttaaaagg
    17821 taaggatgct gcaggtgagt tagaagcgca tcatgccgac tatgttatta atcatttagg
    17881 tgaacttaga ggtgtactag ataatttgta atttgattgt tgtttgacag cataacttgt
    17941 agtgaatgat tgaaccaaag gtttcatatt gagttacaat gaaattaata atgaaaaaat
    18001 gccaagaagc aatggaagta atccaatgtc ttcttggcat tttgaattta cataaattgt
    18061 ttatgactgt accgtcaatt cagttgtgaa aatttgattg tattcaccaa cttgtttaag
    18121 ttcatcaatt atattgtttg aaacaggttg atcaacggat aaaatcatta gcgcatctcc
    18181 gcccgcttca gttctaccta aagtcataga tgcaatgttg atattgtatt tacctaacaa
    18241 tgcgccagtt tttcctacca tacctggagt atcattatga tatgacacaa tttgatattg
    18301 atttggcttt aagtctacag aaaaattatt aattctaaca attcttggac cgaaacctgt
    18361 aaagacagaa gcgccaactt taacggaatc gctatcgttt gatagttcta cctctaagta
    18421 gttactaaaa cctgtctctg ctttattatt ttcaatattt aatgtcactt gttgttgatt
    18481 taacaacatt agagcattga taatattgac ttcaggacca agatcatctt ttaaaatatg
    18541 agtaataatt gtacgtgtta ataaactact atcaatagag gctaagtcgc caccgtacgt
    18601 aattttaata gagcttggtg cattgtacat taattgaata gctaattcac caactgtttg
    18661 gcttaaattg atgaatgatt ttacagtatc atctatattg cttaagtcca ttttaggtgc
    18721 attcactgca tgcgttacag taccatcaat taaaatttcg atgatttcat ttgaaacaga
    18781 aattgccact ttttcttgag cttcgactgt tgaagcaccc aaatgaggtg taacaataat
    18841 tttatcatgt gcaacaagag gcgagtcagt tgcaggttca tgttcaaaca catcgatagc
    18901 tgcccgacta atttgtcctt cgtctaatgc ttttattagc gccttttcat caataatacc
    18961 accacgtgcc acattgatta tttgcaaact aggttttgct ttggcaaaaa agacagcatt
    19021 aattaagcct tttgttttag gtgttagtgg tgtatgtaat gtaacgaaat cagaatgttg
    19081 ggcaatctca tcaactgttg ccttcgtaat gcttaaagat tttgcttttt catccgttaa
    19141 gtaagggtca aaagctagta ttttcattcc gaaactttgc gcacgtttag caacacctaa
    19201 accaattcta ccagcaccaa tgacacctaa tgttttatga taaagctcag tacctttaaa
    19261 tgcatttcga ttccattctt tatttgtaag tgattggtgt gcttgcggaa tatttcgtgc
    19321 cattgataat aacattgcca gtgtatgttc agtagctgaa atcgtattac catctggggc
    19381 attaataact aaaatacctt ttaatgttgc agcatcaata ttaatattat ctacaccaac
    19441 accggcgcgt gcgattactt ttaaagaatc agcagcattt atgatatttt cagtaaccgt
    19501 agtttgacta cgaacgatta aagcatggta tgaaggtata attttgatta atgcttcttc
    19561 ggacaagcca gtttgaatat ctacattgaa ttgttcgtga tctaataatg ctttgatacc
    19621 atcttttgat atgggatctg caacgagtac attaaattgc ttcatgaata acctccatat
    19681 attttgatat acctttaccg atatagttaa ctttacggtg ttcagttaaa ataatttcta
    19741 aagcagatac taccgataaa atatcgaaag gactaatttt ccccatatga ccaattctta
    19801 aaatttgacc tttaagatgg ccttgaccac cagcaattgt tattttaaag cgatttttaa
    19861 gttcatcttt gattattttc acttcatcat ttgtattagg tttgaatgct gtaaccgttg
    19921 gagatgcatc tttatcattg actaataaag ttaaatctaa tgcttttaag gcgcttctta
    19981 atgcatttct aattgcatag tgtcgtgcta ttacgtgatt gaaaccttct gcttttacgg
    20041 tttcaacgta tgcatttaca cctctaaata aagacacatt tggtgtgaac ggtgtagaat
    20101 tgtcagcttg tgacgaaatg tatttattta agtctagata aaattttggc gtagttactt
    20161 ctttgaaatg ttcttttgca cggtggctat aagctacaaa agctaatcct ggaggtaaca
    20221 taattgcttt ttgactacca gaaacaagta catcaatttt atctttgtta atgtcaacat
    20281 caacagcacc aatgcaactt acgccgtcaa ctacaaaata aatattacta ttaaattgat
    20341 taatggcatt tcctaactcg tgaatagggt gtagcactgt cgtagatgtt tcgcaatatt
    20401 gactaaatac tgctttaact tcaacattta aagttgaaag gaaattgatg aaatctttga
    20461 catctacagc ttctccccat gttacgtcat aaatatgcac atttttgtaa taagtttgtg
    20521 caatttgttt aaatcggtta ccaaaggcac ctgaaacaat gacaacgaag tgatcttcag
    20581 ggtttacaat gttcaacata ctagcctcca agacgcttgt accgctagat gttaaaataa
    20641 gtacatcatt ttgactccca aatattggct ttagaccttg aaatgcttgt tgtgcgatgt
    20701 cttcaaaatc tttagaacga tgaccaacca taggtgcttg aatttctctc ataatggcat
    20761 caggtacagg ggttgggcca ggtgttaata acaacggttg atgataatac atttatatgc
    20821 ctcctataaa taagatttca ctattttagc aaattttctg aaaatttaaa agcctaaaaa
    20881 agtgataacg ctatattgtc gaacaaacgt tatcacttaa cgattttatt tggcctgaat
    20941 gatgggatag atttttatgt ccacattatt tcttacagca tttgaaatca tgcaattatt
    21001 atctgcaatt gttatcaatt ttggtaatcg cttttctaat tgtgctattt gatcacttgg
    21061 aatttgaatt tgaggatggt gcacaatttt tgacatactg aattttccgt tatttaaaca
    21121 agctgttcca atcgattgtt gttcaattga aatatctgtg aactttgcac gttcaagagt
    21181 agctgctaat gagatgatat aacatgatga agcggctgat actaacattt catcgggatt
    21241 tgttcctata ccaacaccac ctaaagaagc aggtatagaa atattctctg aaagtatgtc
    21301 gccttgaacg tttccgacat tgttacgacc accttgccaa gaagtttgga ctttaaagtc
    21361 atgttgatgc aattgcttta acctccaata taattgtgat agtttaattt tagaatagtt
    21421 tatcaaagtt taacagaaag gtgacttatc aatgactctg aataaactga aagatgaatt
    21481 acaaattgtt tcgcaccgtg gattgccgag tgattttcct gaaaatacaa tggtcggtta
    21541 tcgagaggta atggggctca atgttgctat gttagaaata gatgttcatt tgaccaaaga
    21601 ccaacatttt gttgtgatac atgatgaaac aattgataga acatcggatg gtaaggggcg
    21661 tattgctgat tacacattat cgcaattaaa atcatttgat tttggtagtt ataaagatgt
    21721 tgcttttaag ggagaacgaa tacccacgtt agatgaagta ctttcattat gcttaaagta
    21781 tgataaaaag ctattgattg aattgaaaag tccgaattta tatccagaaa tagaatgtaa
    21841 attgctggca tttttggaag aaaagaaagt cgatgcgaca caagtagtta tacaatcttt
    21901 tgatatcgaa tgcatcgaaa agttaaacac attgggcagt atatatgaat taggtgtgct
    21961 ttgtagtaaa cgtaattatt ggtataaaaa gccaaacttt tcaagaattg ctcaaattgc
    22021 tagttatgtt aatcctaatt atgcattagt gaccagaaaa tttgttgata aagcacatca
    22081 tcatcaatta caggttatgc catacacagt taataaattg aaaactggag aaaaattaag
    22141 acaaatgggt gtagatggat taattacaga taatccgaaa ttatttatta agtgagacga
    22201 tgaatggtaa tttgatagag ggatgaggat tcgtatatcg caattgataa ttttaaaaac
    22261 taatgatgaa atactaaaga taattaaaca aataagactt gtaaattcta agttgattgt
    22321 aaaatagggt agctccatat aattttgaat ctatcatttt taaattaata aaaaaaacac
    22381 ccacaattgt gggtgtttgt attgaacgta ttatcttgag tagtactcaa cgattaattg
    22441 ttcgttaatt tcagcaggta attcgctacg ttctggtaaa cgtacgaaag taccagttaa
    22501 gctgtcagca tcaaagttta agtactcagg tacgaaattg ttgatttcaa ctgattcaac
    22561 gatgatgttt aatttttgag atttttcacg aactgaaatt gtttgaccag gtttaacaga
    22621 ataagatgga atatcaacac gtttaccatc tactaagata tgaccgtggt taactaattg
    22681 acgtgcttga cgacgagtac gagctaaacc taatgaataa acaacagcgt ctaaacgact
    22741 tgctaataag atcatgaagt tttcaccgtg tacaccgaat tttttaccag cgatgtcaaa
    22801 tgtgttacgg aattgtcttt cagtcattcc atataagtaa cgtaattttt gtttttcacg
    22861 taattgtaaa ccatattctg ataatttttt acgttggttt ggaccatgtt gtcctggtgc
    22921 gtaaggacgt ttttctaatt ctttaccagt accgcttaaa gagataccta aacgacgaga
    22981 ttttttccag tttgaacctc tgaatcgagc cataataaga ctcctccttt aattcttttt
    23041 tgttgttatg aataaacaaa aaagagtgtt gcatgcttac taagatatat tgttttatgt
    23101 gtcttcacct tatagcgaca gttacgtgac acgtccgcat tgggaacaac atagcgctca
    23161 ataatataca actgctactt tcgttaattc acacaaagat tattgtaaca ttttcctgct
    23221 aactgtcaac actttattga aaatgctgat gtccttttat gcgagttgct tttcaataat
    23281 tttaacaatt gcttcaagat gttctttgtc attgtcatca aatcgatccg ttataggggc
    23341 atcgatatct aagacgccga taattttatc atctttgaaa attggaacga caatctcaga
    23401 cttactatta gcatcacaag cgatatgtcc ttcgaattga tgaacatcag ctacaatttg
    23461 tgtacgacgt tctgaaacgg ctgtaccaca tacacctttt ccaattggaa tgtggacaca
    23521 agcggggtgt ccttggaaag gtccaagtat aagttcattt tgttccaata agtaaaagcc
    23581 aacccaatta atttgatcta gattatcatt taataaggca gacatgttac ttaaaatagc
    23641 aatcatgtga tgttcatctt caataaggct tgctgcttgt ttctttaata atgtgtagtt
    23701 tgttgggtta attgttgtca tatttaaaac ctcttatttt catagatttt ataatagtat
    23761 agaatttcga aaatattatg gcaataaatt cattttataa tttatcctta agcaaaatta
    23821 cgttataata gtaatgataa attaggagga gaagcatatg gtgttatata tcattttggc
    23881 aataattgtg attatattga ttgctgtagg tgtattattc tatttacgtt caaataaacg
    23941 acaaattatt gaaaaagcaa tcgaacgtaa aaatgaaatt gagacgttac cttttgatca
    24001 aaaccttgca caattatcta agttgaattt aaaaggtgaa acaaaaacga aatacgatgc
    24061 aatgaaaaag gacaacgtag aaagtacaaa taagtatcta gctcctgtgg aagaaaaaat
    24121 ccataatgct gaggctttat tagataaatt tagtttcaac gcatctcaaa gtgaaattga
    24181 tgatgcaaat gagttgatgg atagttacga acaaagctat cagcaacaat tagaagatgt
    24241 aaatgaaatt attgcgttat acaaagataa tgatgaatta tatgacaaat gtaaggttga
    24301 ttatcgtgaa atgaaacgtg atgttttagc aaatcgtcat caatttggtg aggcagcaag
    24361 tctacttgaa actgaaattg aaaaatttga gccaaggtta gagcaatatg aagtactaaa
    24421 agctgatggt aattatgtac aagcgcacaa ccatatagct gccttgaatg aacaaatgaa
    24481 acagctaaga tcttatatgg aagaaatacc agaattaatt agagaaactc aaaaagaatt
    24541 acctggtcaa ttccaagatt taaaatatgg ttgccgtgat cttaaagttg aagggtatga
    24601 tctggatcac gtgaaagtag acagtacatt acaaagctta aaaacagagc ttagtttcgt
    24661 tgaaccatta attagccgct tagaattaga agaagctaat gataaactag ctaatatcaa
    24721 tgataagtta gatgacatgt atgatttaat tgaacatgaa gttaaagcta aaaatgatgt
    24781 cgaagaaaca aaagatatca ttacggataa cttattcaaa gctaaagaca tgaattatac
    24841 attgcaaaca gaaattgaat atgtacgtga aaactactat ataaatgaat ctgatgctca
    24901 gagtgttcgt caatttgaaa atgaaattca aagtttaatt tctgtatatg atgatatttt
    24961 aaaagaaatg tctaaatctg ctgtgcgata tagcgaggtt caggataatt tacaatattt
    25021 agaagatcat gtcacagtta ttaatgacaa acaagaaaag ctacaaaatc atctgattca
    25081 attgcgtgaa gatgaagcag aagcagaaga caatctgcta cgagtacaat cgaagaaaga
    25141 agaagtgtat cgtcgattac ttgcttctaa cttaacaagc gttcctgaaa ggtttatcat
    25201 catgaaaaat gaaattgatc atgaagttcg tgatgttaac gaacaattta gtgaacgtcc
    25261 aatacacgtt aaacagttaa aagataaagt gtctaaaatt gtgattcaaa tgaatacatt
    25321 tgaagatgaa gcaaatgatg ttcttgttaa tgctgtttat gcagagaaat taattcaata
    25381 tggaaataga tatcgtaagg actatagcaa tgttgataag agcttaaatg aagctgaacg
    25441 attatttaaa aataatcgct ataagcgtgc gattgaaatt gcagagcaag ctcttgaaag
    25501 tgttgagcca ggtgttacta aacatattga agaagaagtt attaagcaat agaaactagt
    25561 atgtagttat acttaaataa tatgagcact ctgtcaaatt ggactgatga gtttaataat
    25621 tgaagttagc caacgatacg ttgtctagct tcttttttat atggataaat gaaagggaca
    25681 ataaatataa atagcaattg tttaaagata aacgtaatca aatgtgttgt tttaattaat
    25741 ataagtagtg aaaaaagcat aatcacacag ctgtttaaat agagtgaaat agtctaattc
    25801 ttatttaata agtagaaata agattatttt gcgctataac ctttattttt tgtcggataa
    25861 tagtataatg cttgagtaaa acaattttaa cgattgatga ttaatgaaag agaagtgttt
    25921 attttgatat atctagataa tgcggcaacg acgaaagcat ttgaagaagt gttagatact
    25981 tatttaaaag taaatcaatc aatgtattat aatccgaata gtccgcataa agctggtttg
    26041 caggcaaatc aattactaca acaagcaaaa acccaaatta atgcaatgat taattcaaaa
    26101 acaaattatg atgttgtatt cactagtggt gcaactgaat ccaataatct tgctttaaaa
    26161 ggtattgcct atcgtaaatt tgatacagcg aaggaaataa ttacatccgt gttagagcat
    26221 ccgtccgtat tagaggttgt aagatatttg gaagcacacg aaggatttaa agttaaatat
    26281 gttgatgtaa agaaagatgg cagtattaac ttagaacact tcaaagaatt aatgtcagac
    26341 aaagtcggtt tagtaacatg tatgtatgta aataatgtaa ctggacaaat acagcctatt
    26401 ccacaaatgg ctaaagttat aaaaaattat cctaaggcac attttcatgt agatgcggtt
    26461 caagcattcg gcaaaatttc aatggatctc aataacatag atagtattag tttaagtgga
    26521 cacaagttta atggtttaaa aggacaaggc gtcttacttg taaatcacat tcaaaatgtt
    26581 gaaccaactg tccatggtgg tggtcaagaa tatggcgtta gaagtggaac agttaatttg
    26641 ccaaatgata ttgcaatggt taaagcgatg aagatagcta atgaaaactt tgaagcattg
    26701 aatgcatttg ttactgagtt aaataatgac gtccgtcaat ttttaaataa atatcatgga
    26761 gtttatatta attcttcaac ttcaggttca ccattcgttt taaatattag ttttcctggc
    26821 gtaaaaggtg aagtattagt taatgctttt tcaaaatatg acattatgat atctacgaca
    26881 agtgcttgtt catctaaacg taataaatta aatgaagtat tggctgcaat gggattatca
    26941 gacaaatcta ttgaaggtag tataagatta tcatttgggg ctactacaac taaagaagat
    27001 atagcgaggt ttaaagaaat atttatcatc atttatgagg aaattaagga gttgctaaaa
    27061 taatgaagta tgatcacttg cttgttagat acggggagtt aacattaaag ggttcaaata
    27121 gaaagaaatt tgtaaatcaa ttaagaaata atgtaaataa gtcattaaaa ggacttgatg
    27181 ggtttgtcgt taaaggcaaa cgagatcgta tgtatattga acttgaagac catgcagata
    27241 taaatgaaat aacatatcga ttatcaaaaa ttttcggtat taaatctatt agtccagtat
    27301 taaaagtaga aaaaacaata gaggcaataa gtgcagcggc aattaaattt gcgcagcaat
    27361 ttgaagaaaa cagcacattt aaaattgatg tgaagcgtgc cgataaaaat ttcccaatgg
    27421 atacgtatga attacagcgt gaattgggtg gtgcagtatt gaagcacttc gacaatattt
    27481 cagtgaatgt caaacgtcca gatcatgaaa ttcgagtgga agttagatta gatgcaattt
    27541 atatgtatga agaagttgtt ccgggttcag gtggattacc agttggtact ggtggtaaga
    27601 cgttactaat gctttcaggc ggtatagact caccagttgc tgggatggaa gtgatgagac
    27661 gtggcgtaac aattgaagcg attcatttcc atagtccacc atttacaagt gatcaagcaa
    27721 aagaaaaagt tattgaattg acacgtattt tagctgaacg tgttggacca attaaattgc
    27781 atattgtacc atttacagaa ttgcaaaaac aggtaaataa agttgtacat ccaagatata
    27841 caatgacttc aacgagacgt atgatgatgc gtgttgctga taaattagta catcaaatag
    27901 gggctttagc tattgtaaat ggtgaaaacc tagggcaggt agccagtcaa acacttcata
    27961 gcatgtatgc aattaataat gtaacttcta ctcctgtatt acgtccttta ttaacttacg
    28021 ataaagaaga aattattatt aaatcgaaag aaattggtac atttgaaaca tctattcaac
    28081 catttgaaga ttgttgtaca attttcaccc ctaaaaatcc agtaaccgaa ccaaactttg
    28141 ataaggtagt ccaatatgaa agtgtctttg attttgaaga gatgattaat cgtgctgttg
    28201 aaaatattga aacacttgaa ataactagtg attataaaac tattaaagaa cagcaaacaa
    28261 accaattaat aaacgacttt ttataaataa aatcctagag taaatttaaa cataagggga
    28321 tgttaaacta tggatttgaa cttaacgatg attataatca taattttatt tggttttatc
    28381 gcggcgttta tagattcggt tgtagggggt ggcggtttaa tttctacgcc agcattatta
    28441 gcaatcggtc taccaccatc tgtggcttta ggtacaaata aattggcaag ttcgtttggt
    28501 tctttaacta gtacgataaa gtttataagg tccggtaaag tggacttata tgttgttgcc
    28561 aaattatttg gttttgtatt tttggcatct gcatgtggcg catatattgc aacgatggtt
    28621 ccgtcacaaa tattgaaacc tttaatcatc attgcacttt cgtcggtgtt tatattcaca
    28681 ttacttaaaa aagattgggg caatacacgc acgtttactc aatttacatt taagaaagcc
    28741 ataatatttg cagcactttt tatattaatc ggcttttatg atggatttgt aggtggggga
    28801 acaggttcat ttatgctttt tgtattgtta gtctttggtt ttgatttttt aagtgcagca
    28861 ggaaatgcta aagttttgaa ctttgcttct aatataggtg cgcttgtatt atttatggta
    28921 ttaggacaag tagattatgt aataggttta attatggcta taagtatgat tgctgggtca
    28981 tatgctgggg ctcattttgc tattaaacaa ggtgttggtt atgtaaaagt attatttatt
    29041 attattactg ctattttaat tttaaaaaat gcttttgatt atattcaaca atttgtataa
    29101 aattaaattt gcttttgatt aaagcatgaa aattgtaatc aaaccataaa ttgtcgtatg
    29161 atgtagttag aattttaaaa tgcaggaggt caagtatatg actgaaataa cattcaaagg
    29221 tggaccaatc cacttaaaag gtcaacaaat taatgaaggt gattttgcac ctgattttac
    29281 agtgttagat aatgacttaa atcaagtaac attagcagat tatgctggta aaaagaaatt
    29341 aattagtgtg gtaccatcaa ttgatacagg tgtttgtgat cagcagactc gcaaattcaa
    29401 ctctgatgct tctaaagaag aggggattgt gcttacaatt tcagcagact taccattcgc
    29461 acaaaaaaga tggtgcgctt cagcaggttt agacaatgtc attacattaa gtgaccaccg
    29521 tgacttatca tttggtgaaa actatggcgt tgttatggaa gaacttcgct tattagctcg
    29581 tgcagtattt gtattagatg cagataataa agttgtttat aaagaaatcg ttagtgaagg
    29641 tactgatttc ccagattttg atgctgcttt agctgcatac aaaaatattt aatcattaaa
    29701 gagataaatc ttaaaatgta tacatcgtgt ccatcgttgt caacagcatt aaaatagaat
    29761 tgttttctat gattgctaag acctatgggc actttttatt ggagagggac gaatatggca
    29821 gaacaacaaa caattatgga acgcttgttt catacattag atgaaaaagc taaaacatta
    29881 aataatgaaa atggccaaag ttttattgaa aatcttgggc tagcaatgga acaagtatat
    29941 accaatgaaa gaggattgtt agaacaatcg acgttacaag atcgacgtaa agcattccaa
    30001 tttgcatatt taagtttaat gcaggaagaa aagatacaag caaatcatca aattacacca
    30061 gattcaattg gattgatact aggattttta gttgagcgtt ttatgaacaa ccaagaagaa
    30121 ttacatattg ttgatattgc aagtggtgcc ggtcatttaa gtgctactgt aaaagaagtg
    30181 ttacctgaaa ttgcggttat gcatcattta attgaagttg atccagtttt atcacgtgtt
    30241 agtgtacatt tagcaaactt cttagaaatt cctttcgatg tgtatcctca agatgccatc
    30301 atgccactac cattagaaga agcagatatc gttattggtg attttccagt aggctattat
    30361 ccaattgatg aaagaagtaa ggagtttaag ctaggttttg aagaaggaca tagttattca
    30421 cattatttat taatagaaca agcaataaat gcattaaaag atgctggata tgcctttcta
    30481 gtggtaccaa gtaatatttt tacaggtgaa catgtaaaac agcttgaaaa atatattgca
    30541 acagagacag agatgcaagc atttttaaat ttaccaccaa ctttatttaa aaatgaaaaa
    30601 gcgcgaaaat ctatattaat tttacaaaag aaaaaatcgg gtgaaacaaa gccagttgaa
    30661 gtattattgg caaatattcc tgatttcaaa attccttcac aatttcaagg atttatgaca
    30721 gagttaaatc agtggatgga cacaaatcgt cctaaaaaat aatctgtaat agtatagtca
    30781 taaaactgta tgaatgataa aatgaaaaat gaaatagtat aaaaagcaaa cgatggagga
    30841 tcattactta tgtcaaaatt aatcttggct atcaatgctg gtagttcatc attaaaattt
    30901 caattaatta gaatgcctga agaggaatta gtaacaaaag gtttagtaga aagaatcgga
    30961 ttgaaggatt caatttttac aattgaagtc aacggggaaa aagttaaaac agtacaagat
    31021 atcaaggatc acgttgaggc agttgatatc atgttggatg cttttaaagc gcacaatatt
    31081 attaatgata ttaacgatat cgatggaaca ggtcaccgtg ttgtacatgg tggtgaaaaa
    31141 ttccctgaat cagtagctat tactgatgag gttgaaaagg aaattgaaga attaagcgaa
    31201 ttagcaccgc ttcacaaccc agctaacttg atgggtattc gtgctttccg taaattatta
    31261 ccaaatatcc ctcatgtggc aatttttgat acagcattcc atcaaacaat gcctgaaaaa
    31321 gcatatctat atagcttgcc atatcattat tataaagatt atggcattcg taagtatggt
    31381 ttccatggta caagccataa atttgtatca caaagagcgg cagaaatgtt agataagcca
    31441 attgaagatt tacgtattat ttcatgtcac attggtaatg gagcatcaat tgcagctatc
    31501 gatggcggta aatctattga tacatcaatg ggcttcacac cacttgctgg tgtaacaatg
    31561 ggtactcgtt ctggtaatat tgaccctgct ttaattccat ttattatgga aaaaacaggt
    31621 aaaactgctg aacaagtatt ggaaatctta aacaaagaat caggtttatt aggattatca
    31681 ggtacatcta gtgatttacg tgacttatca gaagaagctg aatcaggtaa agcacgttca
    31741 caaatggctt tagatgtatt tgcttctaaa attcacaaat atattggatc atatgctgcg
    31801 agaatgcatg gtgttgatgt tatcgtattt acagcaggta tcggtgaaaa ctcagtagaa
    31861 attcgtgcga aagttcttga aggcttagaa tttatgggtg tttactggga tcctaagaaa
    31921 aatgaaaact tactacgtgg taaagaagga tttattaact atcctcattc accagttaaa
    31981 gttgtcgtta ttccaacaga tgaagaaagt atgattgccc gtgatgttat gacattcggt
    32041 ggtttaaaat aatttttaaa taaatattaa gcgtctatct ccgctacaat tatatgtagg
    32101 aggtagacgc tttttgtatt gtttgaaaat taactagtgt aatgtctctt ttcacgaata
    32161 tcttaaagaa ttaagtagtc gaagaagatt atgaaaacat tattaagtga gttttattta
    32221 taaacaataa aaaaccaagt gcagaaagtt gcacttggtt cataatgatc aagtagttat
    32281 ctaatgatca tctatattta attttgatat ttttcacgta attgagttgt tgcaacttgt
    32341 ggttggaagt ctgctggtaa ctcttcagta cgaacaacta acacgtcaca tggcgcatga
    32401 cgaacgatag attctgatac agaaccaaca ataaatcttt ccacggcatt taagcctgat
    32461 gtaccactca taatcaagtc tgcattaatt tcatgtgcaa gctttttagg aatgatagat
    32521 ttaggagagc caaactctag acgcgtttct acatctttaa caccagcgtt agtagctact
    32581 tctttataac catttaataa ttcttctgca aaatgcttag atttttcagt aaattgagca
    32641 tcataaactt cataagaaga atacgttctt gaatcaatta cattcacaat tgttaacttc
    32701 gcatcgttac gtttagcaac accaactgct ctgttaaatg cccattccgc ttcatgtgaa
    32761 ccgtcaactg cgattaaaat atttttgtaa gtaatcatag taagtgcctc cttgttcttt
    32821 cttaattata ttttaccaca attttcggaa aagtcagtgt aataaccttt acaattttgt
    32881 ggatttttga ttgcgctttc aaaatgttcg cgttaagatg attatggagg tggatggtat
    32941 gaaaattggt ataccaaggg agattaaaaa taatgaaaat cgtgttggtt tatcaccaag
    33001 tggtgtgcac gctttagtag aaagtgggca tactgtttta gtggaaacaa atgcgggttc
    33061 aggatcattc tttgaagatg tagattacaa agaagcaggt gctgagattg ttgctgaaca
    33121 agcaaaagtt tgggatgtgg atatggttat taaagttaaa gaaccacttg aatctgaata
    33181 tccatatttt aaagaagggc ttgtattatt cacttatctt catttagcaa atgaagaaaa
    33241 attaacacaa gctttgatag atagaaaagt aattagtatt gcatatgaga ctgtgcagtt
    33301 accagaccga tctttaccat tgttatcacc aatgagtgag gtagcaggaa gaatgtcagc
    33361 tcaagttggc gcagagttcc tacaaaaact taatggtggt atgggaattc tactaggtgg
    33421 tgtcccagga gtacctaagg gtaaagtaac tattatcggt ggtggtcaag caggaacaaa
    33481 tgcagctaaa attgcactag gactaggtgc agatgttacg attttagatg ttaatccaaa
    33541 gcgtttacaa caattagatg atttattcgg tggacgtgta catacaatta tgtcaaatcc
    33601 gttgaatatt gagttgtatg ttaaacaaag tgatttagta ataggtgcag ttttaattcc
    33661 aggtgctaaa gcgccaagac ttgtaacaga agacatgatt aaacaaatga aaaatgggtc
    33721 agttattatt gacattgcta ttgatcaagg cggtattttt gaaacaactg ataaaattac
    33781 gacacatgat gatcctacat atattaagca tggtgtggtt cattatgcag ttgcaaatat
    33841 gccaggtgca gtaccgcgta cttcgacgtt agctttaaat aatgctacgc taccttatgc
    33901 gctcatgcta gctaataaag ggtatagaga agcatttaaa tcaaatcaac cattatcatt
    33961 aggtttaaat acttacaaag gtcacgtaac caataaaggc gttgcagagg catttgaaat
    34021 ggaatataaa tctgtagaag aagcattaca attataattt gaatttgatg aaaagggaat
    34081 ggattacagc gtactaaaga atgacgttat catctccttg gttattaaaa ttaaataatg
    34141 ataaattaaa gctagaaaca atgcgaagaa atcatcgaac atgaatgata ttgatttgat
    34201 tcattacatg ttactgatga acatacctgc atatgtgttt ctagcttgtt tgttacatat
    34261 tgcatttaga cttggataaa aatttatgtc ttaggcgcta caatgttata ttggcagtgt
    34321 ttgactgaat gagaatgcgc ttgtaacaag cttttcattt ttatcccact ccttattttt
    34381 cgtaatgtgt taatacttca tatccttcat tagtgacaag tatgtcatct tcaattctta
    34441 cacctgcaac accaggtaca taaatacctg gttcgattgt aataaccatg ccagcttcta
    34501 acaaattaga attagtactt gaaacatctt gatattcatg ttcttgtaat cctaggccat
    34561 gacctaagcg atgagggaaa tattcaccat aacctttttc tgaaataata tttctagcga
    34621 tatgatcgat atcttttaat ggtattccag gtttaattgc ttggattgca gatgtttctg
    34681 cttctaatac aatattataa atttcttgtg cttctttgct aggttcacca aatttaatag
    34741 tacgtgtcat atcgctacaa taatgctcat aaattacacc taaatcaaat agtacatatt
    34801 cattgctttt taatctgcga tctcctggtg tgccatgagg tgatgcggca tgatctccaa
    34861 ataaaaccat cgtatcaaaa ctcatttcat tgacgccata ttgtttgata gtttgctcaa
    34921 tatggttgac tacttcacat tcagtcacac cttcttttaa ataagaaaca cctatttcga
    34981 tacacttatc tgctaactca gcagctttac gtattttgct aatttcatct tcggatttaa
    35041 tatttctcaa ttgtttgatt gttaaatcaa catctccgaa tgaattgaca ttgaaaccag
    35101 agattaattg tttttggcgt gctactgtta agtgctcgct ttcaattagt aatttattga
    35161 ttgtttgagg ataaagtgaa aaagggtttt cagtgtctaa atatccaacg atttcacctg
    35221 tgaaaggtga tgctttgact tcttcgactt ccatttttgg acaaaatagt acttgtttac
    35281 catctttctt aatcaataat gcaaataatc tttcatgggg ttcgctacgg tatccagtaa
    35341 aataatatac attcaacggt gttgtaatcc atgctgcatc agcttgttga ttgttcaatt
    35401 cgtctattat ttttgatatt tttgtcattt tataaacact cctcaacata atattacttc
    35461 cattttatgc caattattat tagatttcaa aatcgagcta atacattaac tagattttca
    35521 atttttaaaa tttgagaatt tgttataatt aagtggcaaa agggtactaa atttatatag
    35581 taattgatag gaggatgctc aaatgaagtt atcatttcat ggtcaatcaa cgatttattt
    35641 agaagggaac aacaaaaagg ttatcgtaga tccgtttatt tcaaataacc ctaagtgtga
    35701 tttgaatatt gaaacagtgc aggtagacta tatcgtttta acgcacggtc attttgatca
    35761 ttttggtgat gttgtggaac tagcaaagaa aacaggagca acagtaattg gaagtgcaga
    35821 aatggctgac tatctttctt catatcacgg tgttgaaaat gttcatggta tgaatattgg
    35881 tggtaaagca aactttgatt tcggaagcgt taaatttgtt caagcatttc atagttcaag
    35941 ttttacacat gaaaatggta ttccagttta tttaggtatg ccaatgggta tcgtgtttga
    36001 agttgaagga aaaacaattt atcatactgg tgatacaggt ctgtttagtg acatgtcact
    36061 tattgcgaaa cgtcatccag ttgatgtatg ttttgtacca attggagata attttacgat
    36121 gggtattgat gatgcaagtt atgctattaa tgaatttatt aaaccaaaaa tttctgtacc
    36181 gattcattat gatacattcc cattaatcga acaagatcca caacaattta aagatgctgt
    36241 taatgtaggg gacgtgcaaa ttttaaaacc aggtgaatct gttcagtttt aaatagaata
    36301 tagattttac actaaaaaac acctcgctaa tctatgtgga agttatcgtc tgcatatttt
    36361 tagtgggtgt ttttatattc tactgattca gttacgacgt ggacggggga catcagtggt
    36421 tctttatcat ttctgaccta ctctcgtaat tttagtttta tacaattaga cattgattat
    36481 aaatttacta cgatataaaa aatccagaac cacagtcata atcgtttctt gtatttactc
    36541 agtcgtttga gactgcatca tgattgaaag attatttgtg attctggata aaaattttat
    36601 ttaacgatta atacagggat agtcgcacgt tttgctactt tgtggctgac acttccaagt
    36661 acaaatttct ttttgtcttc tgctttacgg ttacttaaaa caacaatttc gtatttaccg
    36721 ctattagcat gttttaatag ttcttctttt gcattaccac gaacaattat ttggtcataa
    36781 tcaataccat aatcttctaa agctttgcgt gttttttcca atcgcttgct tcgttcttca
    36841 gtaagtttgt ttaaatgaac acctgcttta attgatgctt gagcatcttg ttcgctgatt
    36901 gcgtttaaaa ctgttacgac tgtaccttcg ccagctaatt tagacacttc ttttagtgct
    36961 ttttcatttt ttaactgagt gtctacacca agtaatatat ttttatacat tagtacatcc
    37021 ctcctcactt acattctaat aaataataat ggatttttcc atttaaaatt aaagattttg
    37081 tgatctttgt gtttgttgta tattttcaaa aaataaaata ggtgaaacga tcattatttc
    37141 atttcacttc aatgcttttt atccaaaata aagttataat gtagttaata ctgttcgaaa
    37201 agaagaggta aagaatgaca aaacatgaac aaattttaga ttatattgaa tcgttatcta
    37261 taggctctaa aatttcggtg cgtaaaatcg ctaaattttt aaacgtatct gaagggacag
    37321 catatcgtgc aattaaagat gctgataaaa tgggcatggt cgcaacgata gatcgtgttg
    37381 gtacggttcg aatcgaaaaa cgtaatcgta atgaaattga acatttaact tttaatgaaa
    37441 ttgtcaatat tattgatggc caagtgttag gtggcaacaa aggtatcact aagatggtat
    37501 ctaaatttgc aattggagca atggaactca aagatatttt aaagtatatt ggtccaaaga
    37561 cattattgat tgtcggaaat cgtgaagatg tgcagattga agctttaaaa agaggtacgg
    37621 ctatattaat tactggtgga tttaagccat ctaataaagt tattaatttt gcgaatgaac
    37681 atgatttacc tgttctatca tcaagttatg atacattcct agttgcaaat attatcaaca
    37741 aagcactttt taatcagaaa atacgaaaag atatcttaat agttcaagat ataatgacgc
    37801 cattagatga tttatctgtg ctgtttgata cgatgaaaat agcagattat aagcgtatgg
    37861 cgaatcgaac tggccataca agatttccgg tagtcaatga gtcgtataaa ttagtaggta
    37921 ttgtaacaag tcgagagatg attaatacaa aggacgacga cgaaatagat aaagtgatga
    37981 cgcgaaatcc tatttatgta aatgctatga gtacagtagc aagttgtgcg catatgatga
    38041 tttgggaagg tattgaattg attcctgtcg tttcgagtaa taaaaagaca gtgggtgtga
    38101 ttaatagaca agatgtttta aagtctatgc aacttttagg aagacagcct cagatgggtg
    38161 aaacaattaa cgatcaaatc gccaaatata taacaatgaa tcaagatgga attacagtag
    38221 aagtttcacc acttttaatc aatcattacg gaacagtaag taaagcggca tttgtaagta
    38281 ttatagaaga aacaatacaa tatgaaatgc gtaaatttaa aaaaggtaat gtcatgatag
    38341 agaacttaaa tatcgtgtat attaaaacag tacctattga atcacatata acagtgcgct
    38401 ttggcatact agacgtcgga agaaacttcg ccaaaataga agtgaacatg catagtcaaa
    38461 acgacaaagt agcgagcgct ctcgtgatat gccaaatgtt tgacgaagta taggaactta
    38521 gcagagaaaa taggcataag atagatttcg cacaaacgtt ggggccccac cccaacttgc
    38581 attgtttgta gaaattaggg gtccaatttc tctgtgttgg ggcccctgaa ctaaatagta
    38641 tgaaaatata tatatctaaa taattaaaac gggcaaatag tttaatatgg tagtaacaat
    38701 ttttataatt tagatatttt gatatagact aatgcttaaa ttaagcaact tttctaaaaa
    38761 agggagtatg aaaatgatta gtactttgaa tgaaattatg aaatgtatcg aagacaacga
    38821 tacaattatt atacacagac atgttagacc agatcctgat gcatatggtt cacaacttgg
    38881 tttaaaatac tatattcagc agaaatttcc gcaaaagcaa gtatttgcag tgggtgaagc
    38941 ggaatcatca ttaagtttta ttggagagtt agataacatt gatgataaaa catatcaaga
    39001 tgcgcttgta attgtatgtg atactgccaa tgctccacga attgacgatg aacgttatag
    39061 tacaggtcgt aaacttatta aaatagatca tcatcctgca gttgatcagt atggtgatat
    39121 taatttagtt aatacgaacg cgtcatctac aagtgaaatc atttacgatt taatctcaca
    39181 ttttaatgat gaagcaattg ttaataaaga tatcgcgagc gttttatatc ttggtatcgt
    39241 cggtgatact gggcgattcc tttttaacaa tacctcagaa catactatgg aaattgctgg
    39301 aaagttaatt gggcatgata ttgatcataa tgcgctttta aataaaatga tggagaagga
    39361 cccaaaaatg ttgccgtttc aaggttatgt tttacaacat ttcgaactta tggatgatgg
    39421 attctgccaa gttaaaataa ctgaagatgt attggagcaa ttcggtattc agccaaatga
    39481 agcatctcag tttgttaata caattgctga catcaaaggc ttgaaaatat gggtatttgc
    39541 agtcgatgaa ggtaatgaaa tcagatgtcg attacgttct aaagggcaat tgattattaa
    39601 tgatattgcg caagattttg gtggcggtgg tcatccgaat gcgtcaggag tttcagtgga
    39661 cagctgggat gaatttgagc aacttgctac agctttacgc acaaaactta actaatagaa
    39721 aggggccatt caatcatggt ggcatattta aatattcata cggcttatga tttgttaaat
    39781 tcaagcttaa aaatagaaga tgccgtaaga cttgctgtgt ctgaaaatgt tgatgcactt
    39841 gccataactg acaccaatgt attgtatggt tttcctaaat tttatgatgc atgtatagca
    39901 aataacatta aaccgatttt tggtatgaca atatatgtga caaatggatt aaatacagtc
    39961 gaaacagttg ttctagctaa aaataatgat ggattaaaag atttgtatca actatcatcg
    40021 gaaataaaaa tgaatgcatt agaacatgtg tcgtttgaat tattaaaacg attttctaac
    40081 aatatgatta tcatttttaa aaaagtcggt gatcaacatc gtgatattgt acaagtgttt
    40141 gaaacccata atgacacata tatggaccac cttagtattt cgattcaagg tagaaaacat
    40201 gtttggattc aaaatgtttg ttaccaaaca cgtcaagatg ccgatacgat ttctgcatta
    40261 gcagctatta gagacaatac aaaattagac ttaattcatg atcaagaaga ttttggtgca
    40321 cattttttaa ctgaaaagga aattaatcaa ttagatatta accaagaata tttaacgcag
    40381 gttgatgtta tagctcaaaa gtgtgatgca gaattaaaat atcatcaatc tctacttcct
    40441 caatatgaga cacctaatga tgaatcagct aaaaaatatt tgtggcgtgt cttagttaca
    40501 caattgaaaa aattagaact taattatgac gtctatttag agcgattgaa atatgagtat
    40561 aaagttatta ctaatatggg ttttgaagat tatttcttaa tagtaagtga tttaatccat
    40621 tatgcgaaaa cgaatgatgt gatggtaggt cctggtcgtg gttcttcagc tggctcactg
    40681 gtcagttatt tattgggaat tacaacgatt gatcctatta aattcaatct attatttgaa
    40741 cgttttttaa acccagaacg tgtaacaatg cctgatattg atattgactt tgaagataca
    40801 cgccgagaaa gggtcattca gtacgtccaa gaaaaatatg gcgagctaca tgtatctgga
    40861 attgtgactt tcggtcatct gcttgcaaga gcagttgcta gagatgttgg aagaattatg
    40921 gggtttgatg aagttacatt aaatgaaatt tcaagtttaa tcccacataa attaggaatt
    40981 acacttgatg aagcatatca aattgacgat tttaaaaagt ttgtacatcg aaaccatcga
    41041 catgaacgct ggttcagtat ttgtaaaaag ttagaaggtt taccaagaca tacatctaca
    41101 catgcggcag gaattattat taatgaccat ccattatatg aatatgcccc tttaacgaaa
    41161 ggggatacag gattattaac gcaatggaca atgactgaag ccgaacgtat tgggttatta
    41221 aaaatagatt ttctagggtt gagaaactta tcgattattc atcaaatctt aacacaagtc
    41281 aaaaaagatt taggtattaa tattgatatc gaaaagattc cgtttgatga tcaaaaagtg
    41341 tttgaattgt tgtcgcaagg agatacgact ggcatattcc aattagagtc tgacggtgta
    41401 agaagtgtat taaaaaaatt aaagccggaa cactttgaag atattgttgc tgtaacttct
    41461 ttgtatagac caggtccaat ggaagaaatt ccaacttaca ttacaagaag acatgatcca
    41521 agcaaagttc aatatttaca tccgcattta gaacctatat taaaaaatac ttacggtgtt
    41581 attatttatc aagagcaaat tatgcaaata gcgagcacat ttgcaaactt cagttatggt
    41641 gaagcggata ttttaagaag agcaatgagt aaaaaaaata gagctgttct tgaaagtgag
    41701 cgtcaacatt ttatagaagg tgcaaagcaa aatggttatc acgaagacat tagtaagcaa
    41761 atatttgatt tgattctgaa atttgctgat tatggttttc ctagagcaca tgctgtcagc
    41821 tattctaaaa ttgcatacat tatgagcttt ttaaaagtcc attatccaaa ttatttttac
    41881 gcaaatattt taagtaatgt tattggaagt gagaagaaaa ctgctcaaat gatagaagaa
    41941 gcaaaaaaac aaggtatcac tatattgcca ccgaacatta acgaaagtca ttggttttat
    42001 aaaccttccc aagaaggcat ttatttatca attggtacaa ttaaaggtgt tggttatcaa
    42061 agtgtgaaag tgattgttga tgaacgttat cagaacggca aatttaaaga tttctttgat
    42121 tttgctagac gtataccgaa gagagtcaaa acgagaaagt tacttgaagc actgatttta
    42181 gtgggagcgt ttgatgcttt tggtaaaaca cgttcaacgt tgttgcaagc tattgatcaa
    42241 gtgttggatg gcgatttaaa cattgaacaa gatggttttt tatttgatat tttaacgcca
    42301 aaacagatgt atgaagataa agaagaattg cctgatgcac ttattagtca gtacgaaaaa
    42361 gaatatttag gattttatgt ttcgcaacac ccagtagata aaaagtttgt tgccaaacaa
    42421 tatttaacga tatttaaatt gagtaacgcg cagaattata aacctatatt agtacagttt
    42481 gataaagtta aacaaattcg aactaaaaat ggtcaaaata tggcattcgt cacattaaat
    42541 gatggcattg aaactttaga tggtgtgatt ttccctaatc agtttaaaaa gtacgaagag
    42601 ttgttatcac ataatgactt gtttatagtt agcgggaaat ttgaccatag aaagcaacaa
    42661 cgtcaactaa ttataaatga gattcagaca ttagccactt ttgaagaaca aaaattagca
    42721 tttgccaaac aaattataat tagaaataaa tcacaaatag atatgtttga agagatgatt
    42781 aaagctacga aagagaatgc taatgatgtt gtgttatcct tttatgatga aacgattaaa
    42841 caaatgacta ctttaggcta tattaatcaa aaagatagta tgtt
//
LOCUS       DANJFZ010000009        41457 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.009, whole genome shotgun sequence.
ACCESSION   DANJFZ010000009 DANJFZ010000000
VERSION     DANJFZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.009"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            150..662
                     /locus_tag="PWN45_000382"
     CDS             150..662
                     /locus_tag="PWN45_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDL0622181.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            1004..1744
                     /locus_tag="PWN45_000383"
     CDS             1004..1744
                     /locus_tag="PWN45_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622182.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            1758..2732
                     /locus_tag="PWN45_000384"
     CDS             1758..2732
                     /locus_tag="PWN45_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0622183.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            2729..3490
                     /locus_tag="PWN45_000385"
     CDS             2729..3490
                     /locus_tag="PWN45_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622184.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            3503..4534
                     /locus_tag="PWN45_000386"
     CDS             3503..4534
                     /locus_tag="PWN45_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL0622185.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            4751..5110
                     /locus_tag="PWN45_000387"
     CDS             4751..5110
                     /locus_tag="PWN45_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDL0622186.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            5283..6308
                     /locus_tag="PWN45_000388"
     CDS             5283..6308
                     /locus_tag="PWN45_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDL0622187.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            6694..7944
                     /locus_tag="PWN45_000389"
     CDS             6694..7944
                     /locus_tag="PWN45_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0622188.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            8391..15566
                     /locus_tag="PWN45_000390"
     CDS             8391..15566
                     /locus_tag="PWN45_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDL0622189.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            15579..16223
                     /locus_tag="PWN45_000391"
     CDS             15579..16223
                     /locus_tag="PWN45_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL0622190.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(16551..17030)
                     /locus_tag="PWN45_000392"
     CDS             complement(16551..17030)
                     /locus_tag="PWN45_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDL0622191.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(17300..18064)
                     /gene="argB"
                     /locus_tag="PWN45_000393"
     CDS             complement(17300..18064)
                     /gene="argB"
                     /locus_tag="PWN45_000393"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL0622192.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            complement(18080..19321)
                     /gene="argJ"
                     /locus_tag="PWN45_000394"
     CDS             complement(18080..19321)
                     /gene="argJ"
                     /locus_tag="PWN45_000394"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDL0622193.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(19333..20364)
                     /locus_tag="PWN45_000395"
     CDS             complement(19333..20364)
                     /locus_tag="PWN45_000395"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL0622194.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            complement(20403..21587)
                     /gene="rocD"
                     /locus_tag="PWN45_000396"
     CDS             complement(20403..21587)
                     /gene="rocD"
                     /locus_tag="PWN45_000396"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL0622195.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            complement(21840..23195)
                     /gene="brnQ"
                     /locus_tag="PWN45_000397"
     CDS             complement(21840..23195)
                     /gene="brnQ"
                     /locus_tag="PWN45_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0622196.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(23471..24028)
                     /locus_tag="PWN45_000398"
     CDS             complement(23471..24028)
                     /locus_tag="PWN45_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDL0622197.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(24095..25735)
                     /locus_tag="PWN45_000399"
     CDS             complement(24095..25735)
                     /locus_tag="PWN45_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDL0622198.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            25929..26030
                     /locus_tag="PWN45_000400"
     CDS             25929..26030
                     /locus_tag="PWN45_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622199.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(26009..28054)
                     /locus_tag="PWN45_000401"
     CDS             complement(26009..28054)
                     /locus_tag="PWN45_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDL0622200.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            28640..29695
                     /locus_tag="PWN45_000402"
     CDS             28640..29695
                     /locus_tag="PWN45_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0622201.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            29695..30591
                     /gene="murQ"
                     /locus_tag="PWN45_000403"
     CDS             29695..30591
                     /gene="murQ"
                     /locus_tag="PWN45_000403"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0622202.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            30603..32057
                     /locus_tag="PWN45_000404"
     CDS             30603..32057
                     /locus_tag="PWN45_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0622203.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            32060..32935
                     /locus_tag="PWN45_000405"
     CDS             32060..32935
                     /locus_tag="PWN45_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0622204.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(33129..33410)
                     /locus_tag="PWN45_000406"
     CDS             complement(33129..33410)
                     /locus_tag="PWN45_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622205.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            33623..36412
                     /locus_tag="PWN45_000407"
     CDS             33623..36412
                     /locus_tag="PWN45_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0622206.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            36617..37459
                     /locus_tag="PWN45_000408"
     CDS             36617..37459
                     /locus_tag="PWN45_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDL0622207.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            37461..38384
                     /locus_tag="PWN45_000409"
     CDS             37461..38384
                     /locus_tag="PWN45_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL0622208.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            38371..39483
                     /locus_tag="PWN45_000410"
     CDS             38371..39483
                     /locus_tag="PWN45_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HDL0622209.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(39748..41340)
                     /locus_tag="PWN45_000411"
     CDS             complement(39748..41340)
                     /locus_tag="PWN45_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622210.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            41297..>41457
                     /locus_tag="PWN45_000412"
     CDS             41297..>41457
                     /locus_tag="PWN45_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622211.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLES"
BASE COUNT        14029 a         6332 c         7844 g        13252 t
ORIGIN      
        1 tacttatacc ctaacatgat ttttatactc tttgaaaata tattttacag aattttatct
       61 aaatatttaa aaaaatatct taatatcctt gtaatccgat aagaattata gtaatatttt
      121 ttcaaccatt gttataggag gtcttattaa tgacattatt tttattagaa gctaacaatc
      181 ttgattttgc atcaacgaaa gaagaactag aagcaaaggc agcatcacta tctacgaaga
      241 caattccaac attaattgaa gtacaagcta ctgaaaattt aactcatggt tattttattg
      301 tggaagcaaa tgacgaagca gaagctaaac aatttttaac agaagcagat attagtattc
      361 aattagtcaa agaagtacgc ttagttggta aagatttaga tgaagttaaa aatggtgatg
      421 cacatgttga ttaccttgta acttggaaca ttccggaagg cattacgatg gatcaatatt
      481 tagcacgtaa aaagaaaaat tctgttcatt atgaagaagt gccagaagtt gaatttaaac
      541 gcacatatgt atgtgaagat atgtctaaat gtatttgttt atacaacgca cctgatgaag
      601 aagcggtacg tcgcgcgcgc aaagcagttg atacaccgat tgatggcatc gaaaaacttt
      661 aataagacaa caagttgatg agatatatgt atataggttt ggcatggatt tcgattgcag
      721 ttaattagaa tagctcaatg ctataaatgt aagtagttga tatgaagaaa ctaatgaact
      781 aaatgcaagt attgtctaaa acaatcattt tattgaaatt tagtagagct gaaattaata
      841 taacgtcgtt aattgaataa cgcttatgtt ataagagcac tcataccaaa ccataatcat
      901 ctatagatat aacaattcac gatataaggg ctgtgtttgg catagccctt tagatataca
      961 cttaattcct attaaaatag taggggttaa aagggggctt gtcatgatta aaattcaaca
     1021 attacaacat cactttggat cacataaagt aattcataac tttaatttgg acattagcaa
     1081 gggagaaata gtcactttca tagggaaaag tggttgcgga aagtctactt tactcaatat
     1141 tatcggtgga tttattcatc catcgtctgg tcgtgtcatt attgataacg aaattaaaca
     1201 acagccatct ccagattgtt taatgctatt tcaacatcat aatttgctgc catggaaaac
     1261 gattaatgac aacattagga ttggattaca acagaaaatt agtgatgaag agattaacgc
     1321 acagcttaaa ttagttgatt tagaagacag gggaaagcat tttcccgagc aactgtccgg
     1381 gggtatgaaa caacgtgtgg cactatgtcg agcgcatgtg cataagccta acgttatatc
     1441 gatggatgag ccattaggtg cattagatgc atttacacgt tataaacttc aggatcaact
     1501 agtgcaacta aaacataaaa cgcaatcaac tattatttta gtgacgcatg acattgatga
     1561 agctatttat ctttccgacc gcattgttct gttaggtgaa gggtgcaata ttatttctca
     1621 atatgaaatt acagcatcac atccacgcag tcgtaatgat agccacctac ttaagattcg
     1681 taatgaaatt atggaaacat ttgcattgaa tcatcatcaa gttgaacctg aatattattt
     1741 ataaggagtg agtgacgatg aaaaggttaa gcataatcgt catcattgga atctttataa
     1801 ttacaggatg tgattggcaa aggacgtcta aagaacggtc taaaaatgcc caaaatcagc
     1861 aagtgattaa aattggatat ttgccgatta cacattcagc taatttgatg atgactaaaa
     1921 aattattatc acaatacaat catccgaaat ataaactaga attagttaaa ttcaataatt
     1981 ggccagattt aatggacgca ttaaacagtg gtcgtattga tggtgcatca actttaatag
     2041 agctagcgat gaaatcaaaa cagaagggct caaatataaa ggctgtggca ttgggccatc
     2101 atgaaggcaa tgtcattatg ggacaaaaag gtatgcactt aaatgaattt aataataatg
     2161 gcgatgatta ccattttggt ataccacatc gttattcaac acattatctt ttacttgagg
     2221 aattacgtaa acaattaaag attaaaccgg ggcattttag ctatcatgaa atgtcgccag
     2281 cagaaatgcc agccgcattg agtgaacaca gaattacagg gtattctgta gccgaaccat
     2341 tcggtgcact gggtgaaaag ttaggcaaag gtaagacttt gaaacatggt gatgacgtta
     2401 tacctgatgc gtattgctgt gtgctagtac tgagagggga attgcttgat caacacaagg
     2461 atgtagcgca agcatttgta caagattata aaaagtctgg ctttaaaatg aatgatcgca
     2521 agcaaagtgt agacattatg acgcatcatt ttaaacaaag tcgtgacgtt ttaacacagt
     2581 cagcggcatg gacatcctat ggtgatttaa caattaagcc atccggctat caagaaatta
     2641 cgacattggt aaaacaacat catttgttta atccacctgc atatgatgac tttgttgaac
     2701 cgtcattgta taaggaggca tcgcgttcat gacacgtccc acaaataaca aatttatatt
     2761 acctattatc acatttatta ttttcttagg catttgggaa atggtcatta ttattgggca
     2821 ttaccaacct gtattgttac cgggtcctgc tcttgtagga aaaagtatat ggtctttcat
     2881 tgttactgga gaaattttcc aacatttagc aattagttta tggagatttg tagcgggctt
     2941 tgttgtcgca ttgttggttg ctattccatt gggcttcttg cttggaagga atcgttggct
     3001 atacaacgct atcgaaccgc tatttcaatt gattaggccg atatctccga tagcatgggc
     3061 accatttgtt gttctatggt ttggtattgg tagtttgcca gcgattgcga ttatttttat
     3121 cgctgctttt ttcccaattg tgttcaatac tattaaaggc gttagagaca ttgaacctca
     3181 atatttaaaa atagcagcaa atttaaattt aactgggtgg tcattgtatc gcaatatatt
     3241 atttcccggg gcatttaaac aaatcatggc tgggatacat atggcggtag gaacaagttg
     3301 gatattttta gtttctggtg aaatgattgg tgcacaatcg ggattaggtt ttttaatcgt
     3361 tgatgcacga aatatgttga acttagaaga tgttttagca gcaatattct ttatcggatt
     3421 atttggtttt attattgatc gattcattag ttatattgag cagtttatac ttagaagatt
     3481 tggtgaataa ggagagatga tgatgacttt agaaacgctt atcaaagaac aattagatcc
     3541 tcatttagta gaagttgatg aagggacgta ttatccgaga acatttattc agcaattatt
     3601 tgtagatggt tatttcggtg aggcggcatt gagaaaaaat gctgaagtaa tcgaagctgt
     3661 atcgcagtct tgtttgacaa caggattttg tttatggtgc caattagctt tttcaacgta
     3721 tttagaaaat gccacgcagc cacatttaaa taatgactta caacagcaat tgttatctgg
     3781 agaaatatta ggtgctaccg gattgtctaa tccgatgaag tcatttaatg atttagaaaa
     3841 gttgaacctt gaacacactt atgttgatgg acaattggtt gtcagtggac gtatgccagc
     3901 tgtaagtaat attcaagaag accattattt tggtgcgatt tcgaaacatg aatcatcaga
     3961 tgaatttgtc atgttcattc tacgtgccaa tcaagatggt atcactcttg ttgaaaaaac
     4021 caatttttta ggagtcaacg ggtcagcaac gtatcaaatc acattgaatc aagtcgtagt
     4081 gccacaatca caaattatca cgcatgatgc gaagcagttt gcggcaacta ttcgcccgca
     4141 atttattgct taccaaattc caataggatt aggctcaatt aaaagttctt tagagttaat
     4201 tgatgcattt tcaaatgtgc aaaacggaat aaatcaatat ttagagtatg atgttgaagc
     4261 ttttaaaaaa cgttatcgtc aacttagaga ggaatattat gcaatattag atgacggtaa
     4321 cttaacttca catttaaatg aattaatatc attgaagaag gacatcggct atttattgtt
     4381 agatgtaaat caagcttctg ttgtcaatgg tggttctaga gcgtacacac catattcgcc
     4441 acaagttcgc aagttaaaag aaggattctt cttcgcagca ttgacaccga cattaagaca
     4501 tttaggtaaa cttgaagcag agttgaaggg gtaagtgtga taagctgatt ttttgtttag
     4561 atgcgtttgt tgaaacattt tttaaaataa tataaatctt agtttataaa cattttctgt
     4621 taatttgtta tatcctttta actaggaaaa tatacatttc gtaataataa taatcgttat
     4681 cattgaaaaa gtgttaataa ggtgtataat gaaaatgtga acaattaatg aacttcttat
     4741 tttaaagaag gtgaatacta tagatacgca tactaaagaa caacaattct cgaatctagt
     4801 aagatcttat cgtaaagaat acgtgggtaa aggacccaat agtattcgag tgtcgtttaa
     4861 agataattgg gcgattgcac atatgacagg tgttttgagt aaagttgaga gtttttacct
     4921 aaacgacaaa cgcaatgaat cgatgctcca ttatacacgc acagagaaga ttaaacagat
     4981 gtataaagaa atagatgtaa atgagatgga aagtcttgta ggcgctaagt ttgtaaaatt
     5041 atttacagat attgatttga atgatgatga agtcatttca atatttgttt tcgataagtc
     5101 aatagaataa gtgttgctgg tgtaaggtac acggtgctgt ttgctaactt cgctttgaat
     5161 ttaacaataa ttcaaggggg tggtatgtca aacggtgccg tttttttgtc atatttttaa
     5221 aacaagcaac atgcaacacg tactttaagg aagtcaaaat ttatcattta ggagagatgg
     5281 atatgaaaat cgtagcatta tttccagaag cagtagaagg tcaagaaaat caattactta
     5341 atactaaaaa agcattagga ttaaaaacat ttttagagga aagaggacat gagttcatta
     5401 tattagcaga taatggtgaa gacttagata aacatttacc agatatggat gtgattatta
     5461 gtgcgccatt ttatcctgca tatatgactc gtgaacgtat tgaaaaagca ccgaacttga
     5521 aattagcaat tacagcaggt gtaggatctg accatgtaga tttagcggca gcaagtgaac
     5581 acaatattgg tgtcgttgaa gttacaggaa gtaatacagt tagtgtggca gaacatgcgg
     5641 ttatggattt attaatactt cttagaaact atgaagaagg tcatcgtcaa tcagtagaag
     5701 gtgaatggaa cttgtctcaa gtaggtaatc atgcgcatga attacaacac aaaacaattg
     5761 gtatttttgg atttggtcga attggacaac ttgttgctga aagattagcg ccatttaatg
     5821 taacattaca acactatgat ccaatcaatc aacaagacca taaattgtct aaatttgtaa
     5881 gctttgatga acttgtttca acaagtgatg cgattacaat tcatgcacca ttaacaccag
     5941 aaactgataa cttatttgat aaagatgttt taagtcgtat gaaaaaacac agttatttag
     6001 tgaatactgc acgtggtaaa attgtaaatc gcgatgcgtt agttgaagcg ttagcatccg
     6061 agcatttaca aggatatgct ggtgatgttt ggtatccaca acctgcacct gctgatcatc
     6121 catggagaac aatgcctaga aatgctatga cggttcacta ttcaggtatg actttagaag
     6181 cacaaaaacg tattgaagat ggagttaaag atattttaga gcgtttcttc aatcatgaac
     6241 ctttccaaga taaagatatt attgttgcaa gtggtcgtat tgctagtaaa agttatacag
     6301 ctaaatagaa taaggatgct gggctagcga ttaacgcttt caattttata taaatgaatc
     6361 atataagcac tactgctgtt gtaaagatgg cagtagtttt tttatgatta catctaagta
     6421 tagtcacagc tatgttagga caatgattta acatttacgc acatatgtgt tcacttacgc
     6481 aattattgat aaatttcatt catttggaat aatatatagt catatagtgc taattttttt
     6541 gaggcattat atgcaagatg cattgtaaag tcgatgcatt tgtttgttaa ataactttgt
     6601 ataactaaaa attctttgtt tcaacgtata atctaataat agattttata taaatggtag
     6661 tgtgtatata tgtggaaagg ggtgtagtta ttaatgaaac gcttaagtac gactttgaaa
     6721 gtacgattga ttagcaattt tttacagcta attattacga cagcatttat accgtttata
     6781 gcactatatt taacagatat gttaagtcaa tcaattgtcg gtatatatct tgttggttta
     6841 gtggttctaa aatttccatt gtccattata tctggttacc ttattgagat atttccgaaa
     6901 aagttgctag tacttattta tcaagcgacg atggtgataa tgcttgtgtt catgggcgta
     6961 tttgggtcac atcaattgtg gcaaattatt ggtttttgtg ttgcatatgc catatttaca
     7021 atcgtttggg gattacaatt tccagttatg gacacattaa ttatggatgc aattaccgaa
     7081 gacgtggaac attatattta caagattagc tattggatga caaacttatc ggtagctatt
     7141 ggggcattgt taggtggctt gatgtatggc tacagtatgt tactactttt cttaatagca
     7201 gcttgtatat ttttaattgt actctttatt ttatatattt ggttacctca agaccgaaat
     7261 caagtaaagc aaagtgatga caagaggcat gcaagtcgtt atcaaaaatt acaaataatg
     7321 aatatatttc gcagttataa attagttttg aaagaccgta attatatgtt attgatttcg
     7381 gggttcagta tcatcatgat gggtgaattt tcaatctcct catatattgc tattagacta
     7441 aaggatcagt ttgaaacaat aagtataggt tcatatgata ttacaggtgc taagatgtta
     7501 gcaatcttgc taatgattaa tacggtcgtc gtcattttac tcacgtattc aatctcgaaa
     7561 gttgtattga aaatagattt taaaaaagct ttaatcactg gtttgctgat ttatattgtt
     7621 ggctatagtg gtctaaccta tcttaatcag tttggcttat tagttgtttt tatgataatt
     7681 gcgactgtag gtgaaattat ttattcgcct atagtttcag aacaacgctt taaaattatt
     7741 cctaaagcta aaagaggaac atatagtgca gttaatgcat taggtattca tttttcagaa
     7801 acactagcta ggttagggat tgtgttgggt gttttcttaa cgtcattaca aatgggactg
     7861 tatatgttta tcgttttaac aattggtgct agcatgcttg ttgctggtgt atttggggga
     7921 caaaaacaag tgaatacaaa ttgaatttat ttaaatattt attttatgca tctttttata
     7981 tggaaaacct attaatttgg tcgtaattaa aatagagata aatttattaa gaatttgtta
     8041 aaatttaagg ataacaattg ttttatgcga gttgtttgtt taatataaac ttgcttcatg
     8101 gatattttgt aacaacaata ttgagtttgc aatgagatgt gaacaacaga aagtgattaa
     8161 cgagttgtag tgtgctattt atcgaagatg tatgtaattt acattgaaaa atataccaat
     8221 tgtaacgatg atacaagatg tattttaggg tagaatatat cgctcatatg ttgcgaatgg
     8281 tgttttatat taatcattac ataacgagtt aattaacatt tcgtcggtta tcatatcatc
     8341 atgcaatttc agtaagtaat gagtattatg atatgaaaga aggacttttt atgattatgg
     8401 gtaatttgag atttcaacag gaatattttc gtatatacaa aaataataca gaatcaacga
     8461 cacaccgtaa tgcgtattgg gttaaactcg ctaaaaatgt tgaagctact aaaatgatgt
     8521 atgcattatc gacaattgtg caacaacatg catctataag acattttttt gatgttacta
     8581 ccgatgacaa tttaacaatg atacttcatg aatttctgcc ttttattgag ataaaacaag
     8641 ttccatcttc ttccgcaaac tatgatttag aagctttttt taagcaagaa ttaagtactt
     8701 accattttaa tgattcacct ttattcaaag ttaaattgtt tcagttcgct gatgctgcat
     8761 atatactatt agattttcat gtgtccattt tcgatgatag tcaaattgat atttttcttg
     8821 atgatttatg caatgcatat cgtggcaata ctgttattaa caatactcga cagcatgcac
     8881 atataaatag aaatgatgat aaagacaatc aagatgcatc gcatatagca ttagactcaa
     8941 actattttcg gttagagaat aactctgaca tccatattga tagttatttt ccaattaagc
     9001 atccatttga acaagcttta tatcaaacgt atttgattga tgatatgaca tcaatagata
     9061 tggcatcgtt ggctgttagt gtgtatttag ctaatcatat aatgagtcaa caacatgatg
     9121 tcacattagg tatacatgta ccatcacatt taccaaatga tttacacgga aatattgtgc
     9181 cgttaacgtt aacaatcgat gcaaaagatg tatgtcaacg ttttacaaca gattttaata
     9241 aatgtgtgtt gcaaaatatg tcgcaattac agtgcgcgaa gtcttcgctt tcactagaga
     9301 ctatttttca ttgttatcat catatgatgt cttgttgtaa tgatgttatt gaggatgtac
     9361 atcaaataca tgatgcacat acatctttag cggatattga aatttttcca catcaacacg
     9421 ggttcaaaat tatatataac agtgcagcat atgatttgct ctcaatcgag acgctgagtg
     9481 acttagttcg aaatatttat ttgcaaatta ctgaagaaaa tggaaataaa cgaacaactg
     9541 tagatgaact taatttgatg acagaacgtg atattcaatt atatgacgat atcaatttaa
     9601 gtttgcctga gatagatgat gcgcaaacag ttgttacctt atttgagcaa caagttgaag
     9661 caacgccgaa tcatgtcgct gtgcaatttg acggagtgtt tataacatat caaacattga
     9721 atgcacgcgc gaatgattta gcacaccgtt tgagaaacca gtatggtgtt gaacctaatg
     9781 atcgtgtcgc tgtcatagct gaaaaaagta ttgagatgat aatagcgatg ataggtgtgt
     9841 tgaaagctgg tggggcttac gtgccaattg atccgaacta tccaagtgat cgtcaggagt
     9901 acattttaaa agatgtaacg cctaaagttg taataacgta ccaagcttta tatgaaaatg
     9961 gtaaacaaaa tattaatcac attgatttga ataagatagc gtggaaaaat attgataatc
    10021 tttctaaatg taacacgtta gaagatcatg cttatgttat ttacacgtcg gggacaactg
    10081 gtaaccctaa agggacacta attccgcacc gaggtattgt tcgattggtc catcaaaatc
    10141 attatgtacc attaaatgaa gagacgacga ttttgttatc aggaactata gcctttgatg
    10201 ctgcaacatt tgaaatatat ggtgcattgc tcaatggtgg aaagctgatt gttgctaaaa
    10261 aagaacaatt attaaatcca atagcggtag aacaattaat caatgaaaat gacgttaata
    10321 ctatgtggtt aacctcctca ttatttaatc agattgctag tgaacgaata gaagtattgg
    10381 taccgttaaa gtatttatta attggtggag aagtattgaa tgctaagtgg gtggatttgc
    10441 ttaatcaaaa accgaagcat cctcaaatta ttaatggtta tggaccaact gaaaatacaa
    10501 catttacaac gacgtataat atacctaaca aagttccaaa tcgtattcct attggtaaac
    10561 cgattctggg tactcatgtt tatatcatgc aaggcgagcg tcggtgtggc gttggtattc
    10621 ctggagaatt atgtacaagt ggctttgggt tagctgcagg ttatttaaat cagccagaat
    10681 tgacagcaga taaatttatc aaagattcaa atataaatca gctgatgtat agaagtggtg
    10741 atatcgttcg tttgttaccc gatggcaaca tagattattt atatcgaaag gacaaacaag
    10801 ttaagattcg agggtttagg attgagttgt cagaggttga gcatgcgctc gagcgtatac
    10861 aaggtattaa taaagcagtt gttattgttc aaaatcatga tcaagatcag tatatcgttg
    10921 cttattatga agcgatgcat acattatcac ataataagat taaatcacaa ttacgtatga
    10981 ccttaccgga gtacatgata ccagttaatt tcatgcatat tgagcaaatt cctattacta
    11041 ttaatgggaa attagataag aaggcattgc ctatcatgga ctatgtcgat acggatgcct
    11101 atgtagcacc gagtacagat accgaacact tgctatgcca aatttttgca gatattttac
    11161 atgtgaatca agtaggtatt catgataatt tctttgaatt aggtggccat tcattaaaag
    11221 caacgttagt ggtgaatcgg atagaggcat ctactgggaa acgattacaa attggtgatt
    11281 tattacaaaa gccaactgta tttgaactag cacaagcgat tgctaaggtt caagaacaaa
    11341 actatgaagt gattccagaa actatagtta aagatgatta tgtgctgagc tctgcacaaa
    11401 agcgtatgta tttattatgg aaatcaaacc ataaagatac ggtgtataac gtaccttttt
    11461 tatggcggtt atcatcagaa cttaatgtag ctcaattgcg acaagcagtg cagcgtttga
    11521 tagcgcgaca tgagatttta cgaacacaat atattgttgt agatgatgag gttcgacaac
    11581 gtattgtggc agatgttgca gttgactttg aagaagttaa cacgcatttt acggatgaac
    11641 aagaaatcat gcgccaattt gtagcacctt ttaatttgga aaagccaagt caaattagag
    11701 tgagatacat tagaagtccc ttacatgcat acctctttat agatacgcat catatcatta
    11761 atgacggtat gagtaatata caattaatga atgatcttaa cgcactttat caacataaat
    11821 tattgttacc acttaaattg caatataaag actatagtga gtggatgtcg catcgtgata
    11881 tgacgaaaca tagacaatat tggttatctc aattcaaaga tgaagtacct attttaagct
    11941 taccgacaga ctatgttaga ccaaatatta aaacgacaaa tggagcaatg atgtcattta
    12001 caatgaatca acaaatgaga cagctacttc aaaagtatgt agaaaagcat caaattactg
    12061 attttatgtt ctttatgagt gtggtcatga cgttgttaag tagatatgct cgaaaagatg
    12121 atgttgttgt cggtagtgtg atgagtgcgc gtatgcataa aggcacggag caaatgctag
    12181 gcatgtttgc taatacgttg gtatatagag ggcaaccgtc acctgataaa atgtggacac
    12241 agtttttaca agaggttaag gaaatgagtt tggaggcata cgagcatcaa gaatacccat
    12301 tcgaatgttt agtaaatgac ttagatcaat cacatgatgc ctcacggaat ccattatttg
    12361 atgtcatgtt agtactacaa aacaatgaaa cgaatcatgc tcattttggg catagtaaat
    12421 taacacacat tcaacccaaa tcagtgacgg cgaaatttga tttatctttc atcattgaag
    12481 aagatcgcga tgactataca atcaatatcg agtataatac cgatttatat cactcagaaa
    12541 cagttcgtca catgggtaat caatgtatga ttatgattga ttatattttg aagcatcaag
    12601 atacactaca aatttgtgat ataccaaacg gcacggagga acttctaaat tgggtcaata
    12661 cgcatgttaa cgatcgaatg cttaatgtcc cgggaaataa atctatcata agttacttta
    12721 atgaagttgt ctcacgacaa ggtaatcatg ttgcgctagt catgaatgat ttgacaatga
    12781 cgtatgaaac attacgcaac tatgtggatg ccattgcgca catgctccta tcaaatggtg
    12841 tgggcaatgg tcaacgggtt gccttgttta cagaacgtag ttttgaaatg attgcggcga
    12901 tgttggcgac agttaaagta ggtgcatctt atatacctat cgatattgat tttccgaata
    12961 aacgacaagg tgcaattttg gaggatgcta aagtaactgc agtcatgtct tacggcgttg
    13021 aaattgaaac gacattacca gtcattcaat tggaaaatgc taaaggcttt gttgaatcaa
    13081 aggaaaatga acaatatgat gatttacatg gcaatcaact tgaaaacaca gcgatgttag
    13141 ataatgagat gtatgctatt tacacatctg gtacgaccgg gatgcctaaa ggggttgcca
    13201 tacgacaacg aaatttgttg aatttagtgc atgcatggtc aactgaattg caattaggcg
    13261 acaatgaagt atttttgcaa catgcaaata ttgtttttga tgcatcagtt atggagattt
    13321 attgttgttt gttaaatggt catacgcttg tgattccaga tagagaggaa cgtgttaatc
    13381 cagaacagtt acaacaactc attaataagc atcgtgtgac ggttgcgtcg attccgttac
    13441 agatgtgtag tgttatggaa gacttttata ttgaaaagtt gattacaggc ggggcaacta
    13501 gtacggcatc ctttgttaaa tatattgaga agcattgtgg cacgtatttc aatgcctatg
    13561 gaccatctga gtcaacagtc atcacatcgt attggtcaca tcattgtggt gatttgatac
    13621 ctgagacgat tccaattggc aaacccttat ctaacatcca agtgtatatt atgtcagatg
    13681 gtttgttatg cggtattggt atgccaggcg agttgtgtat tgcaggtgat agtttagcga
    13741 taggatatat taatcgtcca gaattaatgg ctgataaatg gcaaaataat ccatttggta
    13801 aaggaaagtt gtatcatagt ggtgatttag cacgttatac atctgatggt caaattgaat
    13861 ttttaggaag aatagataaa caagtgaaag ttaacgggta ccgtattgaa cttgatgaaa
    13921 ttgaaaatgc aatattagct attcgtggta tatctgattg tgttgtaaca gtaagtcact
    13981 ttgatacgca tgatatattg aatgcttatt atgtcggaga gcaacaagtg gaacaggatt
    14041 tgaagcaata tttaaatgat cagctgccta agtatatgat tcctaagact ataacgcata
    14101 tcgattgtat gccattaacc acgaatgaca aggtggatac tacgcgtttg ccaaatccat
    14161 cacctataca acagtctaat aaagtgtata gcgaaccctc taatgaaatt gagcagacat
    14221 ttgttgatgt atttggagag gtattgaaac aaaatgatgt cggtgttgac gatgatttct
    14281 ttgaacttgg tggtaactca ttagaggcga tgttagttgt ctcgcattta aaacgatttg
    14341 gccatcatat ttcaatgcag acattatacc aatataaaac cgtgcgacag attgttaatt
    14401 atatgtacca aaatcaacaa tcattagttg cattaccgga taatctttcg gaattacaaa
    14461 agattgttat gtctcgttat aacttgggta ttttagagga tagtctaagt catcgacctc
    14521 taggaaatac actattgact ggcgcgacag gttttttagg tgcttatctg attgaagtac
    14581 tacaaggata cagtcatcgc atttattgtt tcatacgtgc tgataatgag gaaatagcat
    14641 ggtataagtt gatgacgaat ttaaatgatt atttttcaga agagacggtt gaaataatgt
    14701 tatcaaacat tgaagtcatt gttggtgatt tcgagtgtat ggatgatgtt gttttaccag
    14761 aaaacatgga tacgattatt catgcaggtg ctcgtacaga tcactttggt gatgatgatg
    14821 aatttgaaaa agtaaatgtt caaggtactg ttgatgtcat acgtttggca caacaacatc
    14881 atgcaaggtt aatatatgtg tctacgataa gtgtgggaac ttattttgat atagacacag
    14941 aagatgtgac attttcagaa gcggatgtct ataaagggca actactaaca tcaccatata
    15001 cacggagcaa attttatagt gaattaaaag tattagaagc tgtaaataat ggcttagatg
    15061 gtcggattgt acgtgttggt aatttgacga atccttacaa tggaagatgg catatgagaa
    15121 atataaagac taaccgtttt tcaatggtaa tgaatgattt gttacaactg gattgtatcg
    15181 gggttagcat ggctgaaatg cctgtagatt tttcttttgt ggatacgact gcaagacaaa
    15241 ttgtcgcatt agcacaggtc aacacaccac aaatcattta ccatgtgcta tcacctaata
    15301 aaatgccggt gaaatctttg ttagaatgcg ttaagcgcaa agaaattgaa ctcgtcagcg
    15361 atgaatcatt taatgaaatt ttacagaaac aagacatgta cgaaacgatt ggattaacta
    15421 gtgttgaccg tgaacaacaa ctagcaatga tagatacaac attaacatta aaaataatga
    15481 atcacatcag tgaaaaatgg ccaacgataa ctaacaattg gctgtatcat tgggcacaat
    15541 atatcaaaac aatattcaat aagtaagtag ggaaagttat gacagtattt gtaatgcaat
    15601 tacagagtaa cttgaaaagt attgaagaat taatatcaca aagtcgttgg tcatataaaa
    15661 aaccgcgtac agtcaactat agatacaatc aagataaact catgcacaga ttgggagata
    15721 ttttagtgca atatggaatt caacatgaca caggtttatt accacatgaa tggcattatc
    15781 acatttcgcc acgaggtaag gcagatattg ttcaacacaa tcgtgatgga cagcccatct
    15841 atgtgagctt atcatatagt tatccttata tcgtgtgtgt tgtcgataaa gaaccagttg
    15901 gtattgatat cgaaaagata tcacaacgtt tagactggcg tacgttagtg acgtgtttct
    15961 ctacaaacga agcacatcaa atatgtagtt taaatgattt ttatcaaata tggacacaaa
    16021 aagaaagttt tacaaaattg attggtgaag gtttaatcaa aggattggac atttatgata
    16081 tgacacaatc acacttttat caatcacgtg aagtgaagtt caaacaattt atttttgatc
    16141 agtttatggt acaggtatgt ttcttaggac aggcaccctg gggttataaa aaagtgtctg
    16201 tatttcagtt attgagtagt taatatgacg tctgacagta tcattgcgca gtgattttaa
    16261 actatactgc ctaaatgttt actgttagat gtttttttat tggaaaaatg aaaggtgaat
    16321 acacgatggc atcattcaaa atcatgacga gattaaagta agtgataagg taaaaatata
    16381 ctgagcaata ttgaaattac cgaaaataat agtttgttac gacgcatttg agactttaat
    16441 gctcacgtaa gttggctgga atcatatcat gtcagttaat aaaataacgt gagcatcaat
    16501 catcatactc aaacgatgat ttaaaactca cgttaatgtt agtcagtgta ttaatttaat
    16561 ttatagcgat agacaaaatg ttgtcgcact aattcaattg tcatcatcca gacgatatgg
    16621 ccaaagaatt ctgacagatg ctcttggaat ggttgatccc acacagcagg tacagtatgc
    16681 atgattggca taatgataag gtggaataat acccaaatag caataccaaa aacagcacct
    16741 tgtcccattg ctaagtaagc gtatttttta actaatatgc agtaaataat tgcaatgacg
    16801 atagaaaaac taaagtggac aataaagctt acccaaggca attccatatt tgaaaatgta
    16861 tatgtttgat gcgtaaactc actactaaat cctaattgtt gcaataactc ttgaggtggg
    16921 ttcgttgcat tacgttctgg tgtgcgaggt ggaaacatga cctcccaacc taattttaca
    16981 attccagata acaagccacc gataattcca gcataaatga atatacccat ttgtcgtttc
    17041 gtcaacttaa ggactcctct ctatttacta tcctcattat agtattttgt gaaaataatc
    17101 acaataaaaa gctttgcaaa acttaaaata ttttgataaa caaatttgta aaaggttatt
    17161 agttttttac atctcatatt gtatcgtact gtattcaaaa ttgttaacga gatgaacggt
    17221 aaataaatgt tttagcgcaa tggataatcg aactggaatc atctcgcgtg tcaaaacttg
    17281 ttaggcctta atttcatagt tatgaattaa ggattgttgt gccaacaaaa tcattattgt
    17341 aaatagattc aatgatattt ggcttgtttc ctgatgcaat gataacttta ggacagccat
    17401 tttcaatcgc atttttggca tctagcactt tgggaatcat acctccataa atatcaccat
    17461 gttcaatata ttgatgaata tcgactaatg gcaattgagg tataacaaca tcattgatga
    17521 gtacacctgc aatattactt aatacataaa taggcgcttt taatgatgat gcaataaaat
    17581 aggcaagcgt gtcagcatta atattgtaaa attctccatc atggttattg aaaccaatcg
    17641 aattgatgat aggtacaaat ttagtacata aatactgtaa agcatcctta tttaaagcgg
    17701 tcggaacacc gacatatcca tattgttgat caaaagatgt aatttcaaac agctgtgcat
    17761 ccaaaccaca taagcctatt gcagaacatt ggtgctggtt aaattgagct actaatgcag
    17821 tgttaacgtc tgcaatgagc gtgtgtttag taatggtcat ggttgcttta tcagtcactc
    17881 ttaggccatt aacaaagtgt ggctcgattt gctggtttga taatgcttca ttaataaatg
    17941 ggccaccgcc atgaacgata atggggtaga tgttgtttga tcgtaaatgc ttaatgttgt
    18001 taataattga tggatgcatg tcactaagtg tactgccacc aattttaatg acaataaatt
    18061 tcatctaacc aacaccacct tatgttcgat atgatgcgtt gatacgcaca taatcatagg
    18121 ataaatcaca accgtatgca gtcgctgcag cgttacctaa accaagctga acgtcaattg
    18181 tgacattttc atgagttaat gtattcgaca tagcttgctc atcaaatagt acagccatac
    18241 ctttatcaac gacaggtatt tggttcagtt gaacatatgt gcagttagga tcaatttcac
    18301 atccgctgta gccaatagct gtaatgattc gaccaaaatt ggcatcttcg ccaaaaatag
    18361 ctgattttac tagatttgaa cttacgatag ttttaccgat ttttcttgca tctgatattg
    18421 atttagcgcc tgacacattg acgctgatta actttgttgc gccttcgcca tctctggcta
    18481 tagctttagc taaaaatgta cagacaaaat tgaatgcatc aacaaatgtt tcccattgtg
    18541 gatggtcttg actaagtatt tggtgttcaa cttggtgatt tgccatgact aataccatgt
    18601 catttgtact tgtatcgcca tcaacagtaa tcatattaaa tgtatggtca gtcgaagatt
    18661 ttaataattg atgaagtgta ttcgattcaa tcgatgcatc ggttgttata aaagcaagca
    18721 tggtagccat atttgggtga atcatacctg aacctttggt gctaccacca attgtaacgg
    18781 ttttaccatc gatttttagt gatacagcga tatgttttgt acaggtatca gttgttaaaa
    18841 ttgcctcgtt aaacgcacct ggcgttgcaa aattagcatc cttaatatgt tcggtcccag
    18901 tcttaatttt atccataggc aaatattcac caatgacccc agttgaagca acagcaacat
    18961 gctcagatgg tatttgaagt tgttgagcaa cccatgtttg tgtttgtcgt gcatcatcta
    19021 tgccttgttg accggtacaa gaatttgcat tagctgaatt aacaacaagt gcttgtaatt
    19081 ttcctttaga cttttgtaaa gtgtcttcag tgacaataag tggtgcagct ttaaactgat
    19141 ttaaagtata tacggcagct gcacttgcca aagacgatga gtaaatccat ccaaagtctt
    19201 ttttgttagc gcgtaaaccg atgtgcatac caccagccgt gaagccttga ggtgtactga
    19261 tatcgccatg tttaataatt gaaaagttat attgttgtga tgtcgtttct tgatgtttca
    19321 ttctaacacc ccttatggat aaactggtga ttgatttagg ccagtcgtca cttcaaaatc
    19381 atataatata tttaaatttt gaatggcttg cccacttgcg cctttgacaa ggttatcaat
    19441 cactgatact aaaattgctg tttgcgttgt ttcatctaca tagatgccga tatcgcagta
    19501 gttactaccg agtacttctt ttgtggttgg aaaagtccca atatctctaa ttctgacaaa
    19561 tggctgatta gcataataag aggtcattaa tttatgtaat gattcagtcg tatattcaga
    19621 tgataatttg acatatattg ttgataaaat acctcgtgtc attggtacga gatgtggtgt
    19681 aaatatgact gatacatctt gacccgcaat gatagataaa tattgctcga tttccggttt
    19741 gtgtttatgg tttccgattg cataagcgct tagattttca ttcatttctg aaaaatgaac
    19801 acgttgtgat aatgaacgac cagcacctga cacgccggtc ttagcatcaa taataataga
    19861 tgacaaatct actatttttt cgctaataag tggatgtaat gctaataatg ttgctgtagg
    19921 gaaacagcca ggattagaaa tgagcttcgt tccattgtta tcaaacgatt gccattctga
    19981 aatgctgtaa atagcatgat tcaaatcatc ttgtgctgca gcagtttctt tgtaatatgc
    20041 ttcatatatt tcacgattct taattctaaa tgcgccagat aaatcgataa catgaatacc
    20101 tttttctact aagggaggga tacatgtttt acttacgggt gctggtgtcg caaagaaaat
    20161 tacatcacag tcattattgt ccactgtaag tgcttcgaaa tgttgcataa tatgttgtaa
    20221 atgtggaaat gttaatttca acggttcatc tacttttgaa tgtgagtaga tgtgtgcaat
    20281 cgttacatga ggatgtgttt gtaacaatcg aattaattca attgcgccat aaccgctacc
    20341 gccaacgata cctactttaa tcatcatgaa tccctcctat gtcatatata aatgattaat
    20401 tgttaatttt taaaaacgtc ttgaaaagct gcaacaattt gatggatttc ctctttatca
    20461 atgactagag gtggagacaa tcgaatgata gtacgatgcg tgtctttgca taagattcca
    20521 cgttgaatca gttgatccac aaaaggtgca gcatctgtgt taagctctat gcctataaat
    20581 aaaccacgac ctctaatttc tttaatacta ggatgtttaa gttgtagcaa cgcttttaat
    20641 aaaaatgaac ctaagcgttc tgatcgttca accagttgtt catctttaag tacatcaagc
    20701 gctgccgtcg atattgcaat ggctaaaggg ttaccaccaa atgttgaacc atgtgtacct
    20761 ggtgttagaa cacgcatgac atcattattt gcaagtacag cagatacagg gtataagccg
    20821 ccacccaatg ccttacctaa aatataaatg tctggaacga cttgctccca ttccatagca
    20881 aaccatttcc cagttctacc aagaccaact tgaatttcat ctgcaatcaa taatatttga
    20941 tgtttatcac atagttgacg cacagcttga atatatcctt tcggtggtat attaacgcca
    21001 ccttcacctt gaattggttc caaaataatt gctgctgtat tcggtgaaat agcttgtgtt
    21061 aattgttcaa tgtctccaaa atctactgtt gtagtgcctt gaagtagggg gtgaaatcct
    21121 gctttatatg cgtcgtggtt agatagtgat aatgagccaa gtgtacgacc gtgaaaattg
    21181 ttattcatag cgatgatttc aacttgtccg tcagtaatgc ctttaacttc agagccccat
    21241 tttctagcaa ttttaatggc tgcttcaaca gcttcagtac cagagttaag ggggagtact
    21301 ttgtctttct tagcaagatg acaaattttt tcttcccatt tcccgagatt gtcactataa
    21361 aggacacgtg aaatgataga caactttgaa gcttgttctg tcatcgcttt aacaattgtt
    21421 ggatgacaat ggccttggtt tgcaactgaa aaacccgaaa tgcaatctat atattgtttg
    21481 ccatcagtat cccaaacttt gacaccttta cctttagaaa tgacaagctt aagtggtgca
    21541 taattattag agctataata atcagttaat tcaatgattg aattcatcgg tctccctcct
    21601 ttatatttat acattttaaa gaattaatat taatatatta cagttgttca atataagtgt
    21661 caaatgtaaa tttaataata taaagataag gttgtgtttg tattaatgtg atttgtaaaa
    21721 tgaaagtact aatatttttt gtggataatc attgatatgt atgtatacga ttgcagttta
    21781 atgtagtatg aataaaaaat gccacaagca tatgtttaac atatgactgt ggcatagtat
    21841 tatgcttgtg gaattttacg atgcttaatt ttataaataa tgaagccgat aatgaaacca
    21901 atcaaactga gaacaaccca gcccatacca atgtctgata atggtaaata tttttggctg
    21961 aaattaatca aagtttgtga gaatgatgtg cttgaaatga actctggact agcttttaat
    22021 ccatctacta atgcagcaat cattgtaaag aaaatggtac attgataaat aagttttgaa
    22081 tgatggaatt tgctactaaa taatgttagt acaatcaagg caattgctaa tggatataag
    22141 aacattaaca ctgggactga gtacataata atcttagtta aaccaacatt cgcgaataag
    22201 aacgaaataa agcttacaac tgttgcaatc gctaggtaat tcattttagg gaaaaggtgt
    22261 tcgaatgttt ctgaaaatgc cgtaatcaaa ccgatggctg tttttaaaca agcaaccata
    22321 acgataagtg acaacaggac gataccgtag ttacctaagt agtattgagt aatttgcgct
    22381 aaggcaatac caccattttc actaagtttg aaatgaccaa tacttaatgt acccatgatt
    22441 gctagtaggg tataaatgat ccccatcata atgatactga tagtaccaga cttaattgtt
    22501 tctttagcga tatcagttgg attttcgata cctaactttt taatcgttgc aacaatgata
    22561 ataccaaatg ccaatgacgc tagcgcatct aaggtattgt atccatctaa aaagccgtta
    22621 aataaggcat gtgattgata ttgtttacta ataggtgcat cagatatgcc acctaatgga
    22681 tggataaaag caaataataa aataattgct aataatacta agaataccgg atttaaaaat
    22741 ttaccgatat attctaaaat tcttgatggc tttctcgcaa aaaaccatgc aatcacaaag
    22801 aagacgaagc taaaaataaa taaatataaa gtgatttgct ttggtgataa aaatggcgaa
    22861 aatgcaattt caaatgatgt cgttgccagt ctaggtaagg cgaaaaatgg tccgataact
    22921 agatataagg caatcgtgaa aatgtaagca tatgttttat taacacgcga tgcaatttca
    22981 aataaaccag atgtctttga aatgccaata gcaatgatac ctagaaatgg taagccaatt
    23041 gctgtaatta aaaatcctaa gttagcgata aaaacgttag aaccagcagc ttgacccaag
    23101 tgtattggga agataagatt gccggcacca aagaataaac caaataacat agaacctata
    23161 aacatgtttt ctttaaatgt tagtttcttc ttcatatatg tatttgttct cctttttaga
    23221 acttataaaa atctacttaa ttaagataat atcaaaaatt aaacataaag tgatacataa
    23281 ttttgcaata aatactaaat tttttgaaaa ttccggaata aatactgttt tttatgtagt
    23341 aggggcgtaa gttaagaacg atttaacaga tatagaatat gttaaatata gaaggttaga
    23401 caacccgacg tattaatact ttgtgaaata aagatccccc tcaacatgat cgaagggggg
    23461 aagtatatgt ttagttcaat atatttagaa agtcgtttgt agttattgtt tgtcccatca
    23521 atgggaatac attatctatt ggaaattgat gtagcgtttc gttttgtgca ctcatcatat
    23581 ctgtaacaaa aaactgattg tagtttaatt gataggcatc tcgcgctgtc gtatctacgc
    23641 caatatgcgt tgcgacacca ccaagaacaa tcgtatcaat tcctcgacgt cgcaattgta
    23701 agtccaaatc tgttcctaca aatgcactaa aatgtcgttt gtctatgaca aaatcgtcat
    23761 ctctcttgtc taataaatga tggaaacgac tgtagtcgtc gccttctttt ggtggtaatg
    23821 agatcattgc atttggttgc aatacatctt taccatcata gaaattcacg cgaacaaaag
    23881 cgataaagcc attgtttttt ctaaaaacat ctattaattt attagcgttt tgaacgacat
    23941 tttcagctgt atatggggca taatccattt taagaatacc ttcttgcagg tcgattaaca
    24001 ctaaagcggt tttattaaaa ttaatcatct tatatcagag tcctattcgt ttaataaagt
    24061 attcgcttaa acttataccc tgtatacgat gaaattattt attttgttgt gaaaaagctt
    24121 tagcgatatc gatgagtttc ttcggtgcgt cttcgacagc cattttgact tcgacaaaat
    24181 gcatcacatc gggatgacca ttaattgcat taaacgtgtc ttgtaaatct tttgatgatt
    24241 caacgtcatg aatttcaaca tttttaccac caaatacagc tggtaaagct ttataatccc
    24301 acatgtgaat ttcattataa ggttcataca tgccgtgaat aagtcgttct accgtatagc
    24361 cgtcattatt aatcacaaat aataccggtt taatatgctg tctaatcata gttgaaatag
    24421 cttgaacagt tagttgcaat gagccatcac caattaataa taagttacga cgatctttgt
    24481 ctgctaattg tgaacctaat gttgcaggta atgtatagcc gatagaaccc cataacggtt
    24541 gccctataaa agtattgttt ttgtataatg ctaaatcata agcaccaaag aatgatgtac
    24601 cttgatcagc aatgatgaca tcatttggtt ttaagaaatt ttgcatcatt ttaaaataag
    24661 tttgttgtgt taatggttct gtgccaacag tataatcggg tgatgttgga cgatgatacg
    24721 cagggaacgt tgcgttattc gtatatgaaa tattggataa ctgttttaac aatgatggta
    24781 gagatatttc atcatttgta acatcgtcaa ttttgatatt gtgatgattt aacataacga
    24841 catcatcgat attgaattgg tatgaaaaac ctgctgttgc tgaatctgtt aatttggctc
    24901 caatatttaa aattaaatcg ctgttgtcca cataatctcg tattttatct tcggcaattt
    24961 tcccatcgta aatacccata taatatggat tttcctcatt aaaagcacct tttcctaatg
    25021 aaagttgtgc tactggtatc tgtgtttgat ttacaaaatc ttctaattct tgatggaggt
    25081 gaaaactgtt aatttcatgt ccagtaatga tgataggctg cttcgcttga tgcagtttag
    25141 ttgctaataa ctctatatat gttgatgcat ccgtatattt agttgccgtc acttcaaatg
    25201 gtgtcggtat ctcaatttca gagattgcga catcgattgg taaatgtaaa tgaactgggc
    25261 gtctttcggc gattgctgta ttaattaaac gtggtatttc ggttgttgca ttttcaggtg
    25321 tgatataacc ttgtgcaacg gttatatgtg caaacatttt tcgatagtcg tcaaatgtac
    25381 cttcaccaag tgagtgatgt acatatttac cgccttgttc aacagcacgt gtcggcgcac
    25441 ctgtaatcgc aatgacaggt atgcgttcag catatgaacc tgcgataccg ttgacggcac
    25501 ttaattcgcc aacaccaaat gtagtaacta atgcagcgag tccattaaga cgggcataac
    25561 cgtccgctgc gtaacttgcg tttaattcat ttgtatttcc tacccaatct acattgggat
    25621 tgctgataat atcgtctaga aaagcgagat taaaatcacc aggaacacca aaaattttat
    25681 cgacgcctgc tcgatgaata gcgtcaatta agtaagctcc aatgcgttgt ttcataaaaa
    25741 aagccacctt ttctctatat ttacacttaa aatgatagcc tcgaatatag tgtaaggtca
    25801 aggaagttga ctttgttgtg aaaacgctaa caatagtgaa caattaatat tttttaggaa
    25861 atgagtatga aggggggctt taattgatga tcttaaaatt caaaatttaa ggggagagcg
    25921 aactatcaat gatgctgcaa aatacaatag caaagaaaaa acaggcgcgt caccttatga
    25981 aggtatacgc acctgtttat agtaagcatt atttagcttc aaataattga tcgccaaatg
    26041 aaatgttgcc atgttcacct tgtttaaaat caaggtttgt aatgtttcct tgtgtcacga
    26101 taataggcgt aatatcactc tttgcatgat tgcggatgta gtctaaatca aagttgatta
    26161 ataaatcacc ttgtttaact tcttgacctt cctcaacatg taaagtaaag ccttctccgt
    26221 ttaatttaac agtgtctaaa ccgatgtgga ttaatagttc taaaccacta tctgatacaa
    26281 gaccaattgc atgttttgtt gggaaaatca tttgtacttt accgttgaat ggtgcacgaa
    26341 cttcaccttg tgaaggtttg atagcgatac cgtcacccat cattttttcg ctgaacactt
    26401 gatcaggcac ttctgataat ggtgttactt caccagttaa tggtgcatgc acgatatggc
    26461 tcaattcgct tgttgcagat ttatcttctg caacaacaac agtttcgtct ttatcgtctt
    26521 ccatagtagt aggattttct actacttgac cattcataat ctgttgcatt tcatgtttga
    26581 tttggtcaga tttaggacca aaaattgctt gcatattatt gccgacttct aatacaccag
    26641 atgcgcctaa atctttcaaa ccaggaacat caactttaga tttgtcgtta acttcaacac
    26701 gtagacgtgt gatacaagcg tctaaatgtt taatgtttgc tttgccaccc atagcttcta
    26761 atactgcata tggtaattca gttgctgaag cagtagccgc ttgtgattgt ttatcttcac
    26821 gacctggtgt tttgtatttt aattttacaa ttaagaatcg gaatacgaag tagtaaataa
    26881 ctgcgtatac aagacctaca ggaatgacta accaccattg tgtcttatta ggtagtatac
    26941 cgagtaagaa gtagtcgatg aaaccacctg agaatgtata acctagatga agatctaata
    27001 agtacaatgt taagaatgat aaaccatcaa gtactgcgtg aataaagaat aataatggtg
    27061 ctacaaataa gaatgagaat tctaatggtt ctgtaatacc agttaagaat gatgttaaag
    27121 cagcagaacc cattaaacct gctactactt tcttattttc aggtttagct gtgtgataaa
    27181 ttgctaaagc tgctgcaggt aaaccgaaca tcataacagg gaattcacct tgcatgaatt
    27241 taccagctgt caaatgtgcg ccttcacgaa tttgttcgat aaagatacgt tggtcaccgt
    27301 gaataatttc accagctgca tttttccatg aaccaaactc gaaccagaac ggtgcgtgga
    27361 aaatgtgatg tagaccgaat ggaattaata aacgcttgat gaaaccaaat aagaatacgg
    27421 caacaccagt atttgaatct aataatcctg tactgaatgc atttaatcct gattgaatcg
    27481 ttggccaaat taatgccatt gggaatgcta aaataaatga tgttgtagcc atcataatag
    27541 gtacgaaacg cttaccagcg aagaaaccta aataagatgg taagttaatg ttatagaact
    27601 tgttataaca ccaagctgcc agggccccga ttataatacc gccgaacaca cctgtttgta
    27661 atgttgggat acctaaaatg ctagcgtaac cactcgctgg atcaccaata ttcttaggtg
    27721 taacttgtaa aaagtcgccc attgttttgt tcatgattat gtaaccgacg aatgctgcga
    27781 tagctgctac gccatcaccg ccagctaatc cgattgcgac acctaatgcg aaaatcatag
    27841 gcaagttatc aaaaatgata ccaccagcac ctgtcattaa tttagcgaca gtttgtacgc
    27901 caccattttg tataaacggc aagtagtgtt gtaatgattc accttgcata gctgtaccga
    27961 tagctaataa cagaccagct gctggtaaaa tcgcaacagg taacattagc gctttaccaa
    28021 tacgttgcaa ttgaccgaaa agtttcttcc tcacttgtcc aacctccaaa gttgtattta
    28081 ttatttaaga ttcaataaaa aaagacacga gcaaatagac tgtgttggta accatcacag
    28141 cttaatttaa ctcatgccta atcttactta gtaacacgtt ggtgtatgta attaatgtaa
    28201 aagaagcaat tgactaagta gtatataaaa ctgatacgtt agtttatcta gcttaccatc
    28261 acatcttatt gaatcttatt tgtttgcgac tcctatttta gcatggtgaa gtatgcgttt
    28321 tcaatacaat tattaaattc aatttcaaat ctcttaattg ttcacagatt attattaaaa
    28381 cataatataa gataatcctg aataaaatgt ataaaagtta ttgtaataat ccagcagtag
    28441 cagctattat cagcacttaa aagaaaggat aatctttgat ggtgtaataa ttaacttgtg
    28501 acagaaatat gtaagattat tacaatgaac aggctttaac tgattaatat tgtaatgtgt
    28561 tttctcttat agtaaaatgt aagcgattac acaactcaac tatattttcc taataattat
    28621 attgttaagg agggctactt tgacaggctt ttcagtgtat ttaggacaac ctttagatga
    28681 agcgtatatt aagcgaatga ttaaacaagg ttaccaaatg atttttacat ctgtacaaat
    28741 accagaagaa gatgacgaga caaaatatca ttatttcaca aaactactca atttattaaa
    28801 acatgaacaa gtgacttacc tcatagatgc taatccatct attttaacac catcttttta
    28861 tgaccatctt cgacaatatg atgcacaatt tatgattcgt atcgatcata gtacatcaat
    28921 tgaggcaatc gaagcgataa tggcacaggg tttaaagtgc tgtttgaatg caagtattat
    28981 ttcccgggaa ttgttaacaa gcttacatca acaattgaat gattttacat tactttcatt
    29041 ttgtcataac tattatccaa gaccagatac gggattatct gttgacttgg tcaataagaa
    29101 aaatgaactc atttatcaat ttaatccaaa ggcacaaata tatggtttta ttgtagggag
    29161 tgatttgcga ggtcctttgc ataaaggctt gccaacaatt gaagcaacga gacatagtca
    29221 tcctgtcgtt gcagctaaat tattacaaga aactggtgta tctgaagtgt tagttggaga
    29281 ctcattgatt gaaatgaggc aggcaaaaca acttatagat ttttgcaagc ataggcattt
    29341 cacgttatgt attgaagaag tgtttgatac gacagtgact taccttttcg atatgtgtca
    29401 taaagtacgc ccggataatc cggaaaatgt cattcgttcg gaaacgtcaa gacaaatatg
    29461 tccacattcg attcaaccac agtttacgac gcaacgacgc attggttcag taaccgttga
    29521 taatttgaat aacggacgtt atcaaggcga aatgcaaatt gtgagacaaa cgcttagtgc
    29581 acatgacaat gtgaatgttg ttgcacaaat tattaaagaa gacttaccac tgttaagttg
    29641 tatcgagccg aatgatacat ttgattttca aaaaactagg gagtgtaaga agtgatggaa
    29701 aatagtacga ccgaagcgcg taatgaagcg acgatgcatc ttgatgaaat gactgtggaa
    29761 gaggctttaa ttacgatgaa taaagaagat cagcaagtcc cgttagcagt tcgaaaggca
    29821 ataccacaat tgacaaaagt aattaaaaaa acaattgcac agtataaaaa gggtggacga
    29881 ttgatttata tcggtgcagg tacaagtgga aggttgggtg tcttagatgc agcggagtgt
    29941 gtacctacat tcaatactga ccctcatgaa attataggta ttattgctgg tggacaacat
    30001 gctatgacga tggctgtaga aggtgcggaa gatcacaaaa aattagcgga agaagatttg
    30061 aaaaatatag atttaacatc aaaagatgtc gttataggaa ttgccgcgag tggcaaaacg
    30121 ccatatgtta taggcggttt aacatttgct aacacaatcg gtgctacaac agtatctatt
    30181 tcatgcaatg aacatgcagt tataagtgaa attgcgcagt atccagtaga agttaaagtt
    30241 ggtccagaag tattaactgg ttcaacacgt ttaaagtctg gtacagcaca aaagttaatt
    30301 ttaaatatga tttcaaccat cacaatggtt ggtgtcggaa aagtttacga taacctcatg
    30361 attgatgtta aagcaaccaa tcaaaaactg atcgaccgtt cagtgcgtat tattcaagaa
    30421 atatgtgcta tcacatatga tgaagcaatg gcgttatatc aggtatctga gcatgatgtg
    30481 aaagttgcga cagttatggg tatgtgtggc atttctaagg aagaagcaac aagacggtta
    30541 ttaaacaatg gtgacattgt taaacgagca atcagagata gacaacctta ggagggattt
    30601 aaatgaccaa agaacaacaa cttgcagaac gaattattgc tgcagtaggt ggtatggata
    30661 atatagatag tgtcatgaac tgtatgacac gtgtgcgtat taaagtatta gatgagaata
    30721 aagtagatga ccaagaacta aggcatattg atggtgtcat gggtgttata cacgatgaac
    30781 gcattcaagt tgtggttgga cctggtacag tcaataaagt ggctaatcat atggcggaat
    30841 taagtggtgt taaactaggt gacccaatac cacaccatca caatgatagt gaaaaaatgg
    30901 actataaatc atatgcagct gataaagcaa aggcgaataa ggaagcgcat aaagcaaaac
    30961 aaaagaatgg taagttgaat aaagtattga aatcaattgc caatatcttt ataccgttga
    31021 ttcctgcatt tattggagct ggattaattg gtggtattgc agcagtactg agtaacttaa
    31081 tggtggcagg ctatatttca ggtgcttgga ttacgcaact tataacagta tttaatgtca
    31141 ttaaagacgg tatgttagca tacttagcta ttttcactgg tattaatgcg gctaaagaat
    31201 ttggtgcgac accaggactt ggtggcgtga ttggtggtac aacgttatta acgggtattg
    31261 ctggtaaaaa tattttaatg aatgtcttca ctggagaacc attgcaacct ggacaaggtg
    31321 ggattattgg cgttattttt gccgtttgga ttttaagtat tgtcgaaaag agattacata
    31381 aaattgtgcc aaatgcgatt gatattattg taacgccgac tattgcattg ttgattgtag
    31441 gactattaac tatctttatc tttatgccat tagcaggttt tgtttcagac agtttagttt
    31501 cagtagttaa cggaattatt agtattggtg gcgtatttag tggatttatc attggtgcaa
    31561 gcttcctacc gttagttatg ttagggcttc atcatatttt tacgccaatt catatagaaa
    31621 tgattaacca atcaggtgct acttacttat tgccaattgc agcgatggct ggtgctggac
    31681 aagtaggtgc cgcattagca ctttgggtaa gatgtaaacg caacacaaca ttacgtaata
    31741 ctttaaaagg tgcattgcca gttggtttcc taggtatcgg agaaccatta atctatggtg
    31801 tgactttgcc attaggtcga cctttcttaa ctgcttgtat tggtggtggt attggtggcg
    31861 ctgtaatagg tggaattgga catattggtg ccaaagcaat aggcccaagt ggtgtgtcac
    31921 tattaccatt aatctcagat aatatgtatt taggttatat tgcaggatta cttgctgcgt
    31981 atgctggtgg attcgtttgt acatatttat ttggaacgac aaaggcgatg cgacagacag
    32041 atttgttggg tgattaatga tgacaaatat tttatatcgc attgataagc agttgagtga
    32101 ttttacgaag acagaaaaga taatcgctga ttacatttta aagaatccac ataaaatcat
    32161 tgatatgact gtgaatgatt tggcagatgt tacgaatgtt agtacagcat caattgttag
    32221 atttagtcgg aaaatgacac atcaaggttt tcaagagcta aagattgcga tatctcgata
    32281 cttacccgaa gatattgcaa ccaatccaca tttagaattg attgaaaatg aatctgtaga
    32341 aactttgaaa aataaaatga ttgctagagc aacgaatacg atgcgatttg tagctactaa
    32401 tattatggat gcgcaaattg atgcaatttg tgatgtgttg aaaaatgcca ggacaatatt
    32461 tttatttgga tttggcgcat cgagtttgac tattggtgat ctttttcaaa agttatctcg
    32521 tattggctta aatgtcaggt tattacatga aacgcattta cttgtgtcaa catttgcgac
    32581 gcatgatgat agagattgca tgatttttgt gacgaatcaa ggtagtcata gtgaattgca
    32641 gtcaattgca caggtggcca cacattacag tattcccatc ataactatat ctagtacagc
    32701 taataatcca gtggctcaaa ttgcagacta tgcattgatt tatggcagaa ctgatgaaaa
    32761 tgaaatgcgt atggcggcta caacgtcact atttgcacag ttattcacgg tagatatatt
    32821 gtactatcga tttgtagcat taaattatca tgcgattcta gattgtataa cccaatcgaa
    32881 aatggcactt gataattaca ggaagcatct tgcgacgata gattttaaac attagatatt
    32941 gtgtgcgttc taacataaag ttagttgcaa cgtaaacaat tgaagttttt gatgtgattc
    33001 agttgcttga tgaaaaattg ttgctacaag tttgtaaaac tggcatagag gctattttga
    33061 aaatgataaa ggcgtgacaa attaaagcaa agttcagatt tgagctttgc ttttttcata
    33121 gatggaaatt aaatgctgaa gaataatttt aatgctaaat gaactaatac gaaggtgcta
    33181 aaagcaatta ttatcaaatt agtgatatga atgaaatgtt taaatcgttt gcgttgattg
    33241 tattcgggac ttttatagag taggtagtag tacataaaga gtatggctgc gaacatgccg
    33301 aatattgaag tgaagttacc tacagtgact gccatgtaat gatttaaagt taaatagtaa
    33361 cttagactaa taataaaaaa taaaatgatt tgataggtaa ttaacaccat tcaagtccct
    33421 ccattaatcg tagacaaaaa atttatcaat gatttgatta tttgtattca aattttagta
    33481 tacgacttac ctcaaagaca aatattaaaa gataagacaa taaaattaga ataaatttac
    33541 gtttgaagat aaaaaacaaa catttttcat taaagtttat gatatattta gagcaataga
    33601 aagtgtatgg aaggggatat gaatggcata ccaaagtgaa tacgcattag aaaatgaaat
    33661 gatgaatcaa cttgaacaat tgggttacga aagagtaacg atacgtgata ataagcaatt
    33721 gcttgataat tttagaacga ttttaaatga gcgtcatgcg gacaaattag aaggcaatcc
    33781 cttaacagat aaagaatttc aacgtctgtt aacgatgatt gatggaaaaa gtattttcga
    33841 gagtgcccgt attttacgtg ataaattacc acttagacgt gatgatgagt ctgagattta
    33901 tttgtcgttt ttagatacga aaagttggtg taaaaataag tttcaagtga cgaatcaagt
    33961 atctgtcgag gatacatata aagcacgtta tgatgtaacg atattaatca acggactacc
    34021 ccttgtccaa gttgaattga aacgtcgagg tattgatatt aatgaggcgt ttaaccaagt
    34081 aaaacgttac cgcaaacaaa attacacagg cttattccgc tacatacaaa tgtttatcat
    34141 tagtaatggt gttgaaacgc gatacttttc taataatgat agcgaactat tgaagagtca
    34201 catgttttat tggagtgata aacagaataa ccgtatcaat acattgcaat cgtttgctga
    34261 gtcatttatg agaccttgtc aattagctaa gatgatatcg cgctatatga ttattaatga
    34321 aacagataga atactgatgg caatgcgtcc gtatcaagtg tatgcggtag aagcacttat
    34381 tcaacaagcg actgagacag ggaataatgg atatgtatgg catacaactg gaagtggtaa
    34441 gacgttgact tcttttaaag cgagtcagat tttatcacag caagatgaca ttaagaaagt
    34501 tatctttttg gttgaccgta aagacttgga tagtcaaaca gaagaggaat ttaataaatt
    34561 tgctaagggt gctgtagaca aaacttttaa tacctcgcaa ctggtacgcc aactaaatga
    34621 taaaagtttg ccacttattg taacgacgat tcaaaaaatg gctaaagcga ttcaagggaa
    34681 tgccccttta ttagaacagt ataaaacgaa taaagttgta tttattattg atgagtgtca
    34741 tcgcagtcaa tttggtgaca tgcatcgtct agttaaacaa catttcaaaa atgcccaata
    34801 ctttggattc actggtacgc cacgttttcc agaaaatagt agtcaagatg gtagaacaac
    34861 tgcagatatt ttcggtagat gcttacatac gtatttaatt agagatgcca ttcatgatgg
    34921 taatgtactt ggtttctcag ttgactatat taatactttt aaaaataaag ctttaaaagc
    34981 agaagataac agcatggttg aagcaattga tacggaagaa gtatggttag cggataaacg
    35041 tgtggaatta gtaacacgac atatcatcaa taatcatgat aaatatacac gtaatcgtca
    35101 atattcaagt atatttacag tccaaagtat tcacgcgctt attaaatatt atgagacatt
    35161 taagcgactt aacaaaaagt tggaacaacc gttaacgata gctggtatat ttacgtttaa
    35221 acctaatgaa gatgatcgtg atggtgaagt gccatatcat tcacgtgaaa aattagagat
    35281 aatgattagt gattataata aaaagttcga gacgaatttt tcaacagaca caactaatga
    35341 gtattttaat catatttcaa aaaacgttaa aaagggcgtt aaagatagta aaattgatat
    35401 cttaatcgtt gttaatatgt tcttaactgg ttttgatagt aaagtactga acactttata
    35461 tgttgataag aatttaatgt atcatgattt aattcaagcg tattcacgta caaatagggt
    35521 tgaaaaagaa tcaaagccat ttggtaaaat tgtaaactat cgtgacttga aaaaagagac
    35581 agacgatgca ctgagagtat tctcacaaac aaatgatacg gatacaattt taatgcgcag
    35641 ttatgaagag tataaaaaag aatttatgga cgcttatcgt gagcttaaaa tgattgtgcc
    35701 gacaccacac atggttgatg acattcaaga tgaagaagag ctaaagcgct ttgttgaagc
    35761 ttatcgttta ttagctaaaa taatattacg tttaaaagca tttgacgagt ttgagtttac
    35821 aattgatgaa attggaatgg atgaacaaga gaatgaagac tataaaagta aatatttagc
    35881 tgtgtacgat caagtaaaaa gagcgacggc tgagaaaaat aaagtatcca ttttaaatga
    35941 tattgatttc gaaatagaaa tgatgcgtaa tgatacgatt aatgtgaatt atattatgaa
    36001 tatattgaga caaattgatc ttgaagacaa agcggaacaa cgtcgtaacc aagaacaaat
    36061 tagacgcatt ttagatcatg cagatgatcc gacattgagg ttaaaacgag atctaattag
    36121 agaattcatc gacaatgttg taccttcttt aaataaggat gatgatatcg atcaagaata
    36181 tgttaatttc gaaagtatta aaaaagaagc ggagttcaaa ggatttgctg gagagagatc
    36241 tatcgatgaa caagccctaa aaacaatttc aaatgactac cagtatagtg gtgttgtaaa
    36301 cccacatcac cttaaaaaaa tgattggtga tttgccattg aaagaaaagc gtaaagcaag
    36361 aaaagccatt gaatctttcg tggcagaaac aactgaaaaa tacggtgtgt aatgattcag
    36421 ccccctcgct agattagtgt agggggcatt attattttct atcttttgtt agttaccatg
    36481 tgtttggcaa ccatcatttg gtttcaatac aattttataa tcgtatgtgt atagaaataa
    36541 tgtctatagg catttaaaat agtttataat attaacagga taagtattaa atgtatgttg
    36601 aaggaggtgc gtagctatgg ataatagaaa tatgattaat cgtgttttta gtcaaaagat
    36661 attacatcaa attgcaatca aaaataaaag tgatgttgtt gatgaggcat atgattttta
    36721 tatacaggga cctaaaaata tcaatgtaat acagaagatg aaatctttat ataactatct
    36781 taaaaagtct tatcgtaacg aatattttta caaaaacaca atacttaata aactccttct
    36841 aggacgacat tctattaata caactactgc actttctgag atgcccatag ggaaaagtat
    36901 tgctgatttt atattgttaa acggcaaagg cgttgtctat gagattaaaa cagaattaga
    36961 taagctggat agattagata atcaaattaa tgattattat gaagtgttta attatgtcgt
    37021 agttattaca aatgacaaac atctgaataa agttatggct agatacaaag atacaacagt
    37081 tggaatttta gtgttaacaa ctagaaatac actgagtgaa gttcaaaaac ctaaagaaaa
    37141 caatagtctc ttaaacacaa aagcgatgta caacttttta cgaaaagaag aaagaaaaag
    37201 agttattgca caaaatcata tggatgtgcc aacttataat gatttcacag agtatgatgt
    37261 gttatttgac gtgtttaaag aaataccaat gacgaaactg cataacaata tgatttctga
    37321 gttgaaaaaa agaggaaaca tgaaagaata caaagatgaa tttttagcag cgccggctga
    37381 aattaagttc ttgttatatt tcgcaaaaat gacaaagaaa gataaaaata aactatatca
    37441 ttttcttaag gaggattaat atgtattatc cttatttgcg tgggaaacaa aatgaactat
    37501 ttgcaattag agaattgtta gagaaaggtt tgattggtga ttgtattcaa cctataattg
    37561 aaccaattaa atatacaacc acatttaaaa atactttgca atactgtggt gaaaaagcat
    37621 tctctataaa tttagtagta aattcaaagt taactgaaga agagattagt aacgaaactg
    37681 ttgcacattt aactgaaata ataacaaaaa acaaaagtgt tattcaaaaa gcttacttgg
    37741 gtccttctga tgaaggcaat gataggttga aacagcaatt ttcaagtaat agtttagcta
    37801 ttttaacaag tgtagatgat tgggaaatgt ttggagataa aaataaactt gaaatggttt
    37861 ttgtaccaga tgatagacac attaaacgta aattgcgtaa tattccaaac aaaggcatca
    37921 ttatggatcc ttttaataaa ctaagtcgta atgttgatta tttagataat gatgacgagt
    37981 tttatagcga cgatcacctt tattataagg aagatggata cgtagcattt tcagactatt
    38041 ctgttatagg tggagaatat gtagacggtg gcttttcgcc attagctatt gcgatacata
    38101 ttgtctattt tgatgaggct aatgagctaa gagttaagca ttttgtctct gattctaata
    38161 atgatagatc aaatccaggt aaaaagtttt ttgaggctgt agataaatta gtaacatggt
    38221 caaaaaactt agatattaaa aatagatctt atgcgcttgg acaatttgaa gaattaaatg
    38281 aaaataataa gtatccagga ttaggtttaa ttaaaaggtt atctatcatg catcacctag
    38341 aaattatgaa tagatacttg gagtctcaaa atgaaaatat gtgaaaaatg ttttaataat
    38401 actgaaatcg tagaaatcat tgcaaatgat aatagcaaat ttgacaattg tgatattgat
    38461 aacaatcatc ttggtgttaa aatatttgat acgactaagg acatagataa attagaactt
    38521 attagagatt atttaagacc agcattagaa ttatatgata ttagtataaa tttaccagat
    38581 acttttagcc caaaagaagg taaaaagatt gaaatagcat taaaagacga ctggagtata
    38641 tttaatgttg aggaagctca aataagttgt attttaaatg aactttttaa agatgatgaa
    38701 aatttagata gacgagtgtt ggaagattta gtaggcgcta aaatcataaa cgataaaaaa
    38761 tatacaaata aaaatctaat tgtagcaaat aatgactggg atggattttg tgagagcttg
    38821 aagtataaaa atagatttca taataatatg atcaatttag agaatttagc gttctttctc
    38881 gatatcacta ctaattacta tagtattgag gaatttagag aaagatttga accattatat
    38941 agatctagaa tagttaagtt cattacagag aaaagtgaac taatgtcacc accaaaggga
    39001 tttgcaacgg caggaagatt gaattctaaa tggataagcg tattatacct tagtacaaaa
    39061 gaacaggtca gtatagaaga agttaaacct aagcataatg acattattta tataggtaag
    39121 gttaagttac aaaaaaacgt gacaaaagag aaattgaaaa ttgcgaattt aactaattta
    39181 actagtaatg caatcaaagc tggcgatgat ggatttagaa agtattttgt aaattaccaa
    39241 acattaaaaa agattcataa aggaataaca aatccaagcg atgaacaagg aatagattat
    39301 ttgccatttc aatatttagc tgattatatt cgaagcctta aatatgacgg gataatgtat
    39361 gagagcatat tacaagatgg tacttttaac tttgtgtttt ttgatcaaag tttatttgaa
    39421 tgtgttaatt atgaaagaaa aagagtaagt gacgttaaat atacattgac taagctaagt
    39481 tgacacactc aaaaatctga atattctaat aaaatataaa taatcccccg cacttcacat
    39541 agtggcgggg gatttattct ggcgttaaaa tgcccaaagt atgggggtga ctcttaaatc
    39601 atggcaaagc acctaaaact tgatatggtg ccgagcccta acttagcaca atatagttag
    39661 tatgacaaag tcatctaaca taacacatca gaagtcgcgc aatttatttc agaccataca
    39721 gtgataaagt tgcacaacgc atgactttta tttagcaata actgctactt ctgaaataag
    39781 ttgctttgca tagtctgact gcggatgttt gataatatct tctgtgttat tcagttcaac
    39841 gatttcgcca tttttcataa ctgcaacgcg atcacatatt tcattgataa cacccatgtc
    39901 atgtgtgatg aataaataag tgatgccgaa gtctaactgt aattgtttta ataactcgat
    39961 gatatctttt tgaattgaaa cgtctaaagc ggacactgcc tcgtcgcaaa caatcacttt
    40021 aggttcaaca gcaagtgctc tcgcgatact tacacgctga cgttgcccac cagataattc
    40081 gtgtggatag cgatataaga aagtttgatc taggccaacc ttttctaaca acgatacgac
    40141 agttttaata atgtcatcat tatctttgac tttcccatga atgattagtg gtcgtttaat
    40201 cacatcaatg actttaaatc ttggattaat agatgcgaat ggatcttgaa aaatcatttg
    40261 tatctcttgt cgtaaagatt tcaattcatc atctttaaat aaacttaatg gtaattcgtt
    40321 ataccaaata aagccttctg acacttcctt tagaccgacg accgtcttag ctaatgtcga
    40381 tttccctgac cctgattcac cgacaatgcc taatgtttcg ccttttctaa tagccaagtt
    40441 aatatcatta actgctcggt ataggctgcc actcggtgat gtgtaatcca cgctcacgcg
    40501 atcgaatttt aataaaatat cattgtttaa cggtcttggc ggacgcgttt gatgaatatc
    40561 aggaatcgca tctattaagc gttttgtata ggtatgttgt ggcgatttaa aaatactttc
    40621 aaccgtgcca ctttcaacga cacttccatc tttcattaca atcacatcgt cgcaaaattg
    40681 atacacagcg cctaaatcgt gagtgataaa aataatagat gtttctgtgt actcataaag
    40741 ggacttcatt aactgcagta attgattttg tgtactggca tctaatgccg ttgttggttc
    40801 atctgcgatt aaaatttgtg gctttaaaat caatgccatt gctatcatga cacgttgacg
    40861 cataccacca gaaagttcat gtggataagc atcaaattgt cgagttgcat gttttatacc
    40921 tactttttct aaaatgtcta ttgtcatcga ctttgcttca gatttagata cacgtttatg
    40981 ttgaaatatt acttctgtaa tttgtttgcc aatcgttaat cttggattca acgaagagag
    41041 tggatcttga aaaatcattg aaatatcctt acctcgaatt tgttgtaacg ctgaagttga
    41101 taaattattt aacgattgcc cattaaaaat aatttctcct gttaatgtgt gatctggata
    41161 atctggtagt agccctaaaa tagatttagc ggtaatactt tttcctgatc ctgattcacc
    41221 aacaatacct aggatatgtt tttttcgtaa ttcgaaagag acgtttttta ccgcttgaac
    41281 tgtagtttca tcataattga attgtacatt cagactgttg acttctaata aatttgacat
    41341 gatgttgtgc ctccctattc acaaatgtgt aaatttattc tatacttttg tatatcatag
    41401 taatctactc aagattattt tacaaggagt gattatcata attacttatt tagaatc
//
LOCUS       DANJFZ010000010        40342 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.010, whole genome shotgun sequence.
ACCESSION   DANJFZ010000010 DANJFZ010000000
VERSION     DANJFZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.010"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            963..2300
                     /locus_tag="PWN45_000413"
     CDS             963..2300
                     /locus_tag="PWN45_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL0622212.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            2302..2919
                     /locus_tag="PWN45_000414"
     CDS             2302..2919
                     /locus_tag="PWN45_000414"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL0622213.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2947..3276
                     /locus_tag="PWN45_000415"
     CDS             2947..3276
                     /locus_tag="PWN45_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL0622214.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            3490..4416
                     /locus_tag="PWN45_000416"
     CDS             3490..4416
                     /locus_tag="PWN45_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL0622215.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            4417..5220
                     /locus_tag="PWN45_000417"
     CDS             4417..5220
                     /locus_tag="PWN45_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL0622216.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            5237..5584
                     /gene="yabA"
                     /locus_tag="PWN45_000418"
     CDS             5237..5584
                     /gene="yabA"
                     /locus_tag="PWN45_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL0622217.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5858..6583
                     /locus_tag="PWN45_000419"
     CDS             5858..6583
                     /locus_tag="PWN45_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL0622218.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6576..6824
                     /locus_tag="PWN45_000420"
     CDS             6576..6824
                     /locus_tag="PWN45_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0622219.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6826..7665
                     /gene="rsmI"
                     /locus_tag="PWN45_000421"
     CDS             6826..7665
                     /gene="rsmI"
                     /locus_tag="PWN45_000421"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL0622220.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7951..9924
                     /gene="metG"
                     /locus_tag="PWN45_000422"
     CDS             7951..9924
                     /gene="metG"
                     /locus_tag="PWN45_000422"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL0622221.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9955..10728
                     /locus_tag="PWN45_000423"
     CDS             9955..10728
                     /locus_tag="PWN45_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0622222.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10895..11431
                     /gene="rnmV"
                     /locus_tag="PWN45_000424"
     CDS             10895..11431
                     /gene="rnmV"
                     /locus_tag="PWN45_000424"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL0622223.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            11442..12335
                     /gene="rsmA"
                     /locus_tag="PWN45_000425"
     CDS             11442..12335
                     /gene="rsmA"
                     /locus_tag="PWN45_000425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL0622224.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            12435..12698
                     /locus_tag="PWN45_000426"
     CDS             12435..12698
                     /locus_tag="PWN45_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL0622225.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            13008..13856
                     /locus_tag="PWN45_000427"
     CDS             13008..13856
                     /locus_tag="PWN45_000427"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL0622226.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13870..14694
                     /gene="purR"
                     /locus_tag="PWN45_000428"
     CDS             13870..14694
                     /gene="purR"
                     /locus_tag="PWN45_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL0622227.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14711..15091
                     /locus_tag="PWN45_000429"
     CDS             14711..15091
                     /locus_tag="PWN45_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL0622228.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            15163..15465
                     /gene="spoVG"
                     /locus_tag="PWN45_000430"
     CDS             15163..15465
                     /gene="spoVG"
                     /locus_tag="PWN45_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDL0622229.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15655..15786
                     /locus_tag="PWN45_000431"
     CDS             15655..15786
                     /locus_tag="PWN45_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622230.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEA"
     gene            15809..17161
                     /gene="glmU"
                     /locus_tag="PWN45_000432"
     CDS             15809..17161
                     /gene="glmU"
                     /locus_tag="PWN45_000432"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL0622231.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            17308..18273
                     /locus_tag="PWN45_000433"
     CDS             17308..18273
                     /locus_tag="PWN45_000433"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0622232.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            18423..19076
                     /locus_tag="PWN45_000434"
     CDS             18423..19076
                     /locus_tag="PWN45_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL0622233.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            19387..19959
                     /locus_tag="PWN45_000435"
     CDS             19387..19959
                     /locus_tag="PWN45_000435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL0622234.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19959..23465
                     /gene="mfd"
                     /locus_tag="PWN45_000436"
     CDS             19959..23465
                     /gene="mfd"
                     /locus_tag="PWN45_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL0622235.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            23455..24981
                     /locus_tag="PWN45_000437"
     CDS             23455..24981
                     /locus_tag="PWN45_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0622236.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24981..26174
                     /locus_tag="PWN45_000438"
     CDS             24981..26174
                     /locus_tag="PWN45_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0622237.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            26171..26434
                     /locus_tag="PWN45_000439"
     CDS             26171..26434
                     /locus_tag="PWN45_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL0622238.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            26452..26844
                     /locus_tag="PWN45_000440"
     CDS             26452..26844
                     /locus_tag="PWN45_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL0622239.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26949..27350
                     /locus_tag="PWN45_000441"
     CDS             26949..27350
                     /locus_tag="PWN45_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL0622240.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            27530..28825
                     /gene="tilS"
                     /locus_tag="PWN45_000442"
     CDS             27530..28825
                     /gene="tilS"
                     /locus_tag="PWN45_000442"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL0622241.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            28830..29369
                     /gene="hpt"
                     /locus_tag="PWN45_000443"
     CDS             28830..29369
                     /gene="hpt"
                     /locus_tag="PWN45_000443"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL0622242.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            29626..31719
                     /gene="ftsH"
                     /locus_tag="PWN45_000444"
     CDS             29626..31719
                     /gene="ftsH"
                     /locus_tag="PWN45_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL0622243.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            31948..32829
                     /gene="hslO"
                     /locus_tag="PWN45_000445"
     CDS             31948..32829
                     /gene="hslO"
                     /locus_tag="PWN45_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL0622244.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            33008..33940
                     /gene="cysK"
                     /locus_tag="PWN45_000446"
     CDS             33008..33940
                     /gene="cysK"
                     /locus_tag="PWN45_000446"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL0622245.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            34156..34959
                     /gene="folP"
                     /locus_tag="PWN45_000447"
     CDS             34156..34959
                     /gene="folP"
                     /locus_tag="PWN45_000447"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL0622246.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            34937..35302
                     /gene="folB"
                     /locus_tag="PWN45_000448"
     CDS             34937..35302
                     /gene="folB"
                     /locus_tag="PWN45_000448"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL0622247.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            35299..35775
                     /gene="folK"
                     /locus_tag="PWN45_000449"
     CDS             35299..35775
                     /gene="folK"
                     /locus_tag="PWN45_000449"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL0622248.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            36169..36261
                     /locus_tag="PWN45_000450"
     CDS             36169..36261
                     /locus_tag="PWN45_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622249.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            36314..37801
                     /gene="lysS"
                     /locus_tag="PWN45_000451"
     CDS             36314..37801
                     /gene="lysS"
                     /locus_tag="PWN45_000451"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL0622250.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            38359..38473
                     /gene="rrf"
                     /locus_tag="PWN45_000452"
     rRNA            38359..38473
                     /gene="rrf"
                     /locus_tag="PWN45_000452"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            38486..38561
                     /locus_tag="PWN45_000453"
     tRNA            38486..38561
                     /locus_tag="PWN45_000453"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38519..38521,aa:Val,seq:tac)
     gene            38578..38653
                     /locus_tag="PWN45_000454"
     tRNA            38578..38653
                     /locus_tag="PWN45_000454"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38611..38613,aa:Thr,seq:tgt)
     gene            38660..38732
                     /locus_tag="PWN45_000455"
     tRNA            38660..38732
                     /locus_tag="PWN45_000455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38693..38695,aa:Lys,seq:ttt)
     gene            38766..38840
                     /locus_tag="PWN45_000456"
     tRNA            38766..38840
                     /locus_tag="PWN45_000456"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38798..38800,aa:Gly,seq:gcc)
     gene            38848..38936
                     /locus_tag="PWN45_000457"
     tRNA            38848..38936
                     /locus_tag="PWN45_000457"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38882..38884,aa:Leu,seq:taa)
     gene            38942..39018
                     /locus_tag="PWN45_000458"
     tRNA            38942..39018
                     /locus_tag="PWN45_000458"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38976..38978,aa:Arg,seq:acg)
     gene            39039..39112
                     /locus_tag="PWN45_000459"
     tRNA            39039..39112
                     /locus_tag="PWN45_000459"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39073..39075,aa:Pro,seq:tgg)
     gene            39136..39211
                     /locus_tag="PWN45_000460"
     tRNA            39136..39211
                     /locus_tag="PWN45_000460"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39169..39171,aa:Ala,seq:tgc)
     gene            39334..>40342
                     /locus_tag="PWN45_000461"
     rRNA            39334..>40342
                     /locus_tag="PWN45_000461"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        14389 a         5558 c         8283 g        12112 t
ORIGIN      
        1 ccatgccgaa cacagaagtt aagctcctta gcgtcgatgg tagtcgaact tacgttccgc
       61 tagagtagaa cgttgccagg caaaaaatgg atgcgatgag ccgcattgag accgcaaggt
      121 ctcttttttt atgtctaaaa cgtcaaaata aaaagcaaac acaaagaaaa atggcttggc
      181 gaagtgaaaa cgtttgaatc tgacgaaacg agaaaagagc gcaacgagtt tagtagagct
      241 aaatgagtaa gcgagagccg aagaagagga aagaagcaag cgattgtcac aagtcaagaa
      301 aggtctttag cgacgatggt agccaactta cgttccgcta gagtagaacg ttgccaggca
      361 agttgagacc gtgaggtctc tttttttatg tctaaaacgt caaaataaaa agtaaacaca
      421 aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga cgaaacgaga aaagagcgca
      481 acgagtttag tagagctaaa tgagtaagcg agagccgaag gagaggaaag aagcaagcga
      541 ttgtcacaag tcaagaaagg ttcttagcga ggatggtagc taacttacgt tccgctagag
      601 tagaacgttg ctagacaaga aatgaatgcg atgagccgca ttgagtgtga aattgatatt
      661 ttaataatgt gcacttttga ttatttaaat ctgtgactag aagtataagt gaagacattc
      721 agaagtatta taaaaagtga acagcagtaa gatagttttt aatcataaat catcttactg
      781 ctgtttttag attttatgtc taatatcttt taaatcgaag tacaaaaagg aaattaatta
      841 ttatacaata gacaagctat tgcataagta acactaactt ttgtcaaaga agtgttacta
      901 tataattaat acttttgaaa gtaactaatt ccaaaacagt gtaaataaag gaagcgtata
      961 tcatgaagca acctatttta aataaattag aaagtttaaa tcaagaagaa gcgatttctt
     1021 tgcatgttcc gggtcataaa aatatgacta tcggtcattt atctcaatta tcaatgacaa
     1081 tggataaaac tgaaatacct ggattagatg atttacatca tcctgaagaa gtcattttgg
     1141 aaagtatgaa gcaggtggag aaacattcag attatgatgc ttatttctta gtgaatggca
     1201 ccacttcagg aatattatct gtcatccagt ctttttcaca gaaaaaaggc gatatcttaa
     1261 tggcaagaaa tgtacataaa tctgtgttac atgcgctcga tattagccaa caagaagggc
     1321 attttattga aacgcatcaa agtccgttaa cgaatcatta taataaagtt aatttaagcc
     1381 gtttgaataa tgacggtcac aaacttgctg tgttgactta tcctaactat tacggtgaaa
     1441 catttaatgt agaagaggtt atcaaatttt tgcaccaatt aaatattcct gtactcattg
     1501 acgaagcaca cggcgcgcac tttggattgc aaggatttcc agattctaca ttaaattatc
     1561 aagctgacta tgttgttcaa tcttttcata aaacgttacc agctttaacg atgggctcgg
     1621 tactttatat tcataaaaat gcaccttata gagaaactat tatagaatat ctaagctact
     1681 tccaaacatc tagtccttcg tatttgatta tggctagttt agagtcagct gccgagttct
     1741 ataaaacata tgatagtacc gtgttttttg ataagagagc gcaattaatc gaatgtttgg
     1801 agaagaaggg ttttgaaatg cttcaagttg atgatccgtt gaagttgctg ataaaatatg
     1861 aaggttttac aggtcatgat attcaaaatt ggtttatgaa tgcacatatc tatttagaat
     1921 tagcggacga ctatcaagca ttagcgatat tgccgttatg gcatcatgat gatacgtatt
     1981 tatttgattc gcttttacgt aaaattgaag atatgatttt accgaaaaaa tcagtttcta
     2041 aagttaaaca aacacaactt ttaacaactg aaggtaacta taaaccaaaa cgctttgaat
     2101 atgttacttg gtgtgatttg aaaaaggcaa aaggtaaagt tctggcgcga catattgtcc
     2161 cgtatccgcc agggattcct attattttca aaggagaaac aataactgaa aatatgatag
     2221 aattggtaaa tgaatatctg gaaactggaa tgatagttga aggaattaaa aataataaaa
     2281 ttttagttga ggatgaataa aatgtcagct tttataactt ttgagggccc agaaggctct
     2341 ggaaaaacaa ctgtaattaa tgaagtttac catagattag taaaagatta tgatgtcatt
     2401 atgactagag aaccaggtgg tgttcctact ggtgaagaaa tacgtaaaat tgtattagaa
     2461 ggcaatgata tggacattag aactgaagca atgttatttg ctgcatctag aagagaacat
     2521 cttgtattaa aggtcatacc agctttaaaa gaaggtaagg ttgtgttgtg tgatcgctat
     2581 atcgatagtt cattagctta tcaaggttat gctagaggga ttggcgttga agaagtaaga
     2641 gcattaaacg aatttgcaat aaatggatta tatccagact tgacgattta tttaaatgtt
     2701 agtgctgaag taggtcgcga acgtattatt aaaaattcaa gagatcaaaa tagattagat
     2761 caagaagatt taaagtttca cgaaaaagta attgaaggtt accaagaaat cattcataat
     2821 gaatcacaac ggttcaaaag cgttaatgca gatcaacctc ttgaaaatgt tgttgaagac
     2881 acgtatcaaa ctatcatcaa atatttagaa aagatatgat ataattgtta gaagaggtgt
     2941 tataaaatga aaatgattat agcgatcgta caagatcaag atagtcagga acttgcagat
     3001 caacttgtta aaaataactt tagagcaaca aaattggcaa caacaggtgg gtttttaaga
     3061 gcgggtaata caacattctt atgtggtgtc aatgatgacc gtgtagatga aatattgtct
     3121 gtgattaatc aaacgtgtgg taatagagaa cagttggttt cacctattac acctatggga
     3181 ggcagtgcgg attcgtacat tccatatcca gttgaagttg aagttggcgg tgctactgta
     3241 tttgttatgc cagttgatgc attccatcaa ttttaattct ataatacaat catcaattag
     3301 agatacttaa aatagtgtat taataagtta ttaacaattt tgggttgctt gactgcgact
     3361 agttcagatg ccaatagatt tgatttttgt ggttctaaaa ataatcacaa atcatggtcg
     3421 ctattgttgc agtaattagt tgctctttgg caaccttttt atataaaagc aaaagggagt
     3481 ttgtaatgaa tggatgaaca gcaacaattg acgaatgcat atcattcaaa taaattatcg
     3541 catgcctatt tatttgaagg tgatgatgca caaacgatga aacaagttgc gattaatttt
     3601 gcaaagctta ttttatgtca aacagatagt caatgtgaaa caaaggttag tacatataat
     3661 catccagact ttatgtatat atcaacaact gagaatgcaa ttaagaaaga acaagttgaa
     3721 caacttgtgc gtcatatgaa tcaacttcct atagaaagca caaataaagt gtacatcatt
     3781 gaagactttg aaaagttaac tgttcaaggg gaaaacagta tcttgaaatt tcttgaagaa
     3841 ccaccggaca atacgattgc tattttattg tctacaaaac ctgagcaaat tttagacaca
     3901 atccattcaa gatgtcagca tgtatatttc aagcctattg ataaagaaaa gtttataaat
     3961 agattagttg aacaaaacat gtctaagcca gtagctgaaa tgattagtac ttatactacg
     4021 caaatagata atgcaatggc tttaaatgaa gaatttgatt tattagcatt aaggaaatca
     4081 gttatacgtt ggtgtgaatt gttgcttact aataagccaa tggcacttat aggtattatt
     4141 gatttattga aacaggctaa aaataaaaaa ctgcaatctt taactattgc agctgtgaat
     4201 ggtttcttcg aagatatcat acatacaaag gtaaatgtag aggataaaca aatatatagt
     4261 gatttaaaaa atgatattga tcaatatgcg caaaagttgt cgtttaatca attaattttg
     4321 atgtttgatc aactgacgga agcacataag aaattgaatc aaaatgtaaa tccaacgctt
     4381 gtatttgaac aaatcgtaat taagggtgtg agttagatgc caaatgtaat aggtgttcag
     4441 tttcaaaaag cgggaaaatt agaatattat acacctaatg atatacaagt agatatagaa
     4501 gactgggtag ttgtcgaatc taaaagaggc atagagatag gtattgttaa aaatccatta
     4561 atggatattg ctgaagagga tgttgtgtta cctcttaaaa atattattcg cattgctgat
     4621 gacaaagata ttgataaatt taattgtaat gaacgagatg ctgaaaatgc attaatacta
     4681 tgtaaagaca ttgtaagaga acaaggtttg gacatgcgtt tagtcaattg cgaatataca
     4741 ttagataaat cgaaagttat ttttaatttt acggcggatg atcgtattga ttttagaaaa
     4801 ttagtaaaaa tattagcgca acatttaaaa acacgtatcg agttgagaca aattggtgta
     4861 agggatgaag ccaaattgct tggcggtatc ggaccttgtg gtaggtcgtt atgttgttct
     4921 acatttttag gggattttga accagtatcg attaagatgg ctaaggatca aaatttatca
     4981 ttaaatccaa ctaaaatttc tggtgcatgt ggtcgtttga tgtgttgttt aaaatatgaa
     5041 aatgactatt atgaggaagt acgtgcacaa ttacctgata ttggtgaagc aattgaaacg
     5101 cctgatggta acgggaaagt agttgcttta aatatattag acatttctat gcaggtgaag
     5161 cttgagggac atgaacagcc acttgaatat aaattagaag aaatagaaac tatgcattaa
     5221 ggaggcatta ttacatttgg atcgcaatga aatatttgaa aaaataatgc gtttagaaat
     5281 gaatgtcaat caactttcaa aggaaacttc agaattaaag gcacttgcag ttgaattagt
     5341 agaagaaaat gtagcgcttc aacttgaaaa tgataatttg aaaaaggtgt tgggcaatga
     5401 tgaaccaact actattgata ctgcgaattc aaaaccagca aaagctgtga aaaagccatt
     5461 accaagtaaa gataatttgg ctatattgta tggagaagga tttcatattt gtaaaggcga
     5521 attatttgga aaacatcgac atggtgaaga ttgtctgttc tgtttagaag ttttaagtga
     5581 ttaatcaagc acactcaaat agtgttataa ttataaatga atatggtttg gataagtctg
     5641 agacaatgca tgtttcaggc tttaattgtg tataaagttt tggtgattgc ataagagatg
     5701 gcggtactaa atgttattat taagtgtgca cgcagtatca ttagttataa aatgtagctg
     5761 ttaaaagtca aaaatacatc gaatgtagtt aggcatataa tataaaaaga gttttcaatt
     5821 actcaataga aaaaggttgt cttcatagga gttaaaaatg ttaaaagaga atgaacgatt
     5881 tgatcaacta atcaaagaag attttagtat tattcaaaat gatgatgttt tttcattttc
     5941 aacggatgct ttgttgttag ggcattttac aaaacctaga acaaaagata ttgtgttgga
     6001 cttatgttca ggcaatgggg tgataccctt gttattgttt gcgaaacatc cacgacatat
     6061 agaaggtgtt gagattcaaa aaacacttgt cgatatggcg cgacgcacat ttcaattcaa
     6121 tgatgttgat gaatatttaa caatgcatca catggatttg aaaaacgtta ctaaagtatt
     6181 taaaccttca caatatactt tagtaacgtg taatccgcct tattttaaag agaatcagca
     6241 acaccaacat caaaaagaag cacataagat agcgagacat gagattatgt gtacacttga
     6301 agattgcatg attgcagccc gtcatttatt aaaagaaggt ggcaggctaa acatggtaca
     6361 tcgtgcagag agactaatgg atgtcttgtt tgaaatgaga aaagtgaata ttgaacctaa
     6421 gaaagtcgtt tttatatata gtaaagtagg gaaatcagca caaacgatag tagtagaagg
     6481 tcgaaaaggt ggaaatcaag gtttagaaat catgccccca ttttatattt ataatgaaga
     6541 tggtaattat agcgaagaaa tgaaggaagt atattatgga tagtcatttt gtatatattg
     6601 taaaatgtag tgatggaagt ttatatacag gatacgctaa agacgttaat gcacgtgttg
     6661 aaaaacataa ccgaggtcaa ggagccaaat atacgaaagt aagacgtccg gtgcatttag
     6721 tttatcaaga aatgtatgag acaaagtctg aagcattgaa gcgtgaatat gaaattaaaa
     6781 cttataccag acaaaagaaa ttgcgattaa ttaaggagcg atagtatggc tgtattatat
     6841 ttagtgggca caccaattgg taatttagca gatattactt atagagcagt tgatgtattg
     6901 aaacgtgttg atatgattgc ttgtgaagac actagagtaa ctagtaaact gtgtaatcat
     6961 tatgatattc caactccatt aaagtcatat cacgaacata acaaggataa gcagactgct
     7021 tttatcattg aacagttaga attaggtctt gacgttgcgc tcgtatctga tgctggattg
     7081 cccttaatta gtgatcctgg atacgaatta gtagtggcag ccagagaagc taatattaaa
     7141 gtagagaccg tgcctggacc taatgctggg ctgacggctt tgatggctag tggattacct
     7201 tcatatgtat atacattttt aggatttttg ccacgaaaag agaaagaaaa aagtgctgta
     7261 ttagagcaac gtatgcatga aaatagcaca ttaattatat acgaatcacc gcatcgtgtg
     7321 acagatacat taaaaacaat tgcaaagata gatgcaacac gacaagtatc actagggcgt
     7381 gaattaacta agaagttcga acaaattgta actgatgatg taacacaatt acaagcattg
     7441 attcagcaag gcgatgtacc attgaaaggc gaattcgtta tcttaattga aggtgctaaa
     7501 gcgaacaatg agatatcgtg gtttgatgat ttatctatca atgagcatgt tgatcattat
     7561 attcaaactt cacagatgaa accaaaacaa gctattaaaa aagttgctga agaacgacaa
     7621 cttaaaacga atgaagtata taatatttat catcaaataa gttaatcact ttatcgatta
     7681 tatgaaattt taaacgattt tataaacgca agctgtaatt ttaaatggta agttatcatt
     7741 ttgcattgat actgataaaa tgatgttgac tatgataaaa aaaatgatga catcgacgtt
     7801 ttttaatgta aaataaatac attgaaagta ataaatacct taacattgaa taagatgaaa
     7861 atgagatgac gagataaatg ttcgcgtccg ttgaaatgca tagaaatctt agatattatt
     7921 tgaagtgaga cattacgagg aggaacagtt atggctaaag aaacatttta tataacaacc
     7981 ccaatatact atcctagtgg gaatttacat ataggacatg catattctac agtggctgga
     8041 gatgttattg caagatataa gagaatgcaa ggatatgatg ttcgctattt gactggaacg
     8101 gatgaacacg gtcaaaaaat tcaagaaaaa gctcaaaaat ctggtaagac agaaattgaa
     8161 tatttggatg agatgattgc tggaattaaa caattgtggg ctaagcttga aatttcaaat
     8221 gatgatttta tcagaacaac tgaagaacgt cataaacatg tcgttgagca agtgtttgaa
     8281 cgtttattaa agcaaggtga tatctattta ggtgaatatg aaggttggta ttctgttccg
     8341 gatgaaacat actatacaga gtcacaatta gtagacccac aatacgaaaa cggtaaaatt
     8401 attggtggca aaagtccaga ttctggacac gaagttgaac tagttaaaga agaaagttat
     8461 ttctttaata ttagtaaata tacagaccgt ttattagagt tctatgacca aaatccagat
     8521 tttatacaac caccatcaag aaaaaatgaa atgattaaca acttcattaa accaggactt
     8581 gctgatttag ctgtttctcg tacatcattt aactggggtg tccatgttcc gtctaatcca
     8641 aaacatgttg tttatgtttg gattgatgcg ttagttaact atatttcagc attaggctat
     8701 ttatcagatg atgagtcact atttaacaaa tactggccag cagatattca tttaatggct
     8761 aaggaaattg tgcgattcca ctcaattatt tggcctattt tattgatggc attagactta
     8821 ccgttaccta aaaaagtctt tgcacatggt tggattttga tgaaagatgg aaaaatgagt
     8881 aaatctaaag gtaatgtcgt agaccctaat attttaattg atcgctatgg tttagatgct
     8941 acacgttatt atctaatgcg tgaattacca tttggttcag atggcgtatt tacacctgaa
     9001 gcatttgttg agcgtacaaa tttcgatcta gcaaatgact taggtaactt agtaaaccgt
     9061 acgatttcta tggttaataa gtactttgat ggcgaattac cagcgtatca aggtccactt
     9121 catgaattag atgaagaaat ggaagctatg gctttagaaa cagtgaaaag ctacactgaa
     9181 agcatggaaa gtttgcaatt ttctgtggca ttatctacgg tatggaagtt tattagtaga
     9241 acgaataagt atattgacga aacaacgcct tgggtattag ctaaggacga tagccaaaaa
     9301 gatatgttag gcaatgtaat ggctcactta gttgaaaata ttcgttatgc agctgtatta
     9361 ttacgtccat tcttaacaca tgcgccgaaa gagatttttg aacaattgaa cattaacaat
     9421 cctcaattta tggaatttag tagtttagag caatatggtg tgcttaatga gtcaattatg
     9481 gttactgggc aacctaaacc tattttccca agattggata gcgaagcgga aattgcatat
     9541 atcaaagaat caatgcaacc gcctgctact aaagaggaaa aagaagagat tcctagcaaa
     9601 cctcaaattg atattaaaga ctttgataaa gttgaaatta aggcagcaac gattattgat
     9661 gctgaacatg ttaagaagtc agataagctt ttaaaaattc aagtagactt agattctgaa
     9721 caaagacaaa ttgtatcagg aattgccaaa ttctatacac cagatgatat tattggtaaa
     9781 aaagtagcag ttgttactaa cctgaaacca gctaaattaa tgggacaaaa atctgaaggt
     9841 atgatattat ctgctgaaaa agatggtgta ttaaccttag taagtttacc aagtgcaatt
     9901 ccaaatggtg cagtgattaa ataactgtat ttttaaaaat taggagagat aattatgtta
     9961 atcgatacac atgtccattt aaatgatgag caatacgatg atgatttgag tgaagtgatt
    10021 acacgtgcta gagaagcagg tgttgatcgt atgtttgtag ttggttttaa caaatcgaca
    10081 attgaacgcg cgatgaaatt aatcgatgag tatgattttt tatatggcat tatcggttgg
    10141 catccagttg acgcaattga ttttacagaa gaacacttgg aatggattga atctttagct
    10201 cagcatccaa aagtgattgg tattggtgaa atgggattag attatcactg ggataaatct
    10261 cctgcagatg ttcaaaagga agtttttaga aagcaaattg ctttagctaa gcgtttgaag
    10321 ttaccaatta tcattcataa ccgtgaagca actcaagact gtatcgatat cttattggag
    10381 gagcatgctg aagaggtagg cgggattatg catagcttta gtggttctcc agaaattgca
    10441 gatattgtaa ctaataagct gaatttttat atttcattag gtggacctgt gacatttaaa
    10501 aatgctaaac agcctaaaga agttgctaag catgtgtcaa tggagcgttt gctagttgaa
    10561 accgatgcac cgtatctttc gccacatccg tatagaggga agcgaaatga accggcgaga
    10621 gtaactttag tagctgaaca aattgctgaa ttaaaaggct tatcttatga agaagtgtgc
    10681 gaacaaacaa ctaaaaatgc agagaaattg tttaatttaa attcataaag ttaaaagtga
    10741 gaaagatcac cgccataaat gtaaacgatg ctatattcgt ttaatatgct atggttcttt
    10801 ctcacttttt taaattaaaa tatcgtgcat gtggaatacg tgcgatagag atggttagag
    10861 ctttgaaatt aagaattgta ggaaggcgtt ttaaatgaaa atcaatgagt ttatagttgt
    10921 agaaggacga gatgatactg agcgtgttaa acgagctgtt gaatgtgata cgattgaaac
    10981 gaatggtagt gccatcaacg aacaaacttt agaagtaatt agaaatgctc aacaaagtcg
    11041 aggcgttatt gtattaacag atccagattt cccaggagat aaaattagaa gtacaattac
    11101 tgaacatgtc aaaggtgtta aacatgcgta tattgataga gaaaaagcta aaaataaaaa
    11161 agggaaaatt ggtgttgaac atgccgaatt aattgatatt aaagaagcgt taatgcatgt
    11221 tagttcaccc tttgatgaag cttatgaatc aattgataaa tctgtgctaa tagagttggg
    11281 gttaattgtt gggaaagatg caaggcgccg tagagaaatt ttaagtagaa aattgcgaat
    11341 cggccattcc aatggtaagc agttattgaa aaagttaaat gcatttggtt ataccgaagc
    11401 ggatgtaagg caagctttag aagatgaatg aggaagtgaa aatgttggat aataaagata
    11461 ttgcaacacc atcaagaacg cgagcgttgt tagataaata tggctttaat tttaaaaaaa
    11521 gtttaggaca gaactttttg atagatgtga atatcattaa taatatcatt gatgcaagtg
    11581 atattgatgc acaaactggg gtgattgaaa ttggtccagg catggggtca ttgacagaac
    11641 aattggccag acatgctaaa agagtattgg catttgaaat tgatcaacgt ttaatacctg
    11701 tattaaatga tacactatca ccttatgata atgtgacggt gattaatgaa gatattttaa
    11761 aagcgaatat taaagaagct gttgaaaatc atttacaaga ttgtgaaaaa ataatggttg
    11821 ttgcaaacct gccgtactat attacgacgc caattttatt aaatttgatg caacaagata
    11881 taccaattga tggctacgtg gtgatgatgc aaaaagaagt gggcgaacgc ttaaatgctg
    11941 aagtaggttc aaaagcatat ggttcgttat caattgtcgt acaatactat acagagacta
    12001 gtaaagtatt aacggtacct aaatctgtat ttatgccacc acctaatgtt gattcaatag
    12061 ttgtaaaact gatgcagaga actgaaccgt tagtaacagt agataacgag gaagcattct
    12121 ttaagttagc aaaagcagca tttgcacaaa gaagaaagac aattaacaat aactatcaaa
    12181 attattttaa agatggtaaa caacacaaag aagtgatttt acaatggttg gaacaagcag
    12241 gtattgatcc aagacgtcgc ggtgaaacgc tatctattca agattttgct aaattgtatg
    12301 aagaaaagaa aaaattccct caattagaaa attaaatgat tgacaaagca aagcactatt
    12361 gttaaaattt aaattttgtt tgacgaaaac gttgcaaata tggtattatg taacttgtag
    12421 cgaggtggag caatatgcca aaatcaattt tggacatcaa aaattctatt gattgtcatg
    12481 taggaaatcg tattgtactg aaagccaatg gaggccgtaa gaaaacaata aaacgttctg
    12541 gaattttaaa agaaacatat ccgtcagttt tcattgttga gttagatcaa gacaaacaca
    12601 actttgagag agtatcttat acatacactg atgtgttaac tgaaaatgtt caagtttcat
    12661 ttgaagagga taatcatcac gaatcaattg cacactaaat aagacatata gagatgttag
    12721 acgtttctta gtataagaag taaatattat gataattatt tgagtgttgg gcattatgtt
    12781 caatactctt tttatttaca aaatgtttaa cactgatgtt tcgcttatag atttttcagt
    12841 aaatggataa ttgtatttat aaacacaaat acaagtaaat actaagtaat tagatggaga
    12901 aaattacttt tttattaaaa aaacactaaa aaacaaatta aaatgtcaaa tattaattct
    12961 ctttatgtta aaatcatcat attaagataa cgaaaagagg gcggaaaatg atatatgaaa
    13021 cggcaccagc caaaattaat tttacgctcg atacactttt taaaagaaat gatggctatc
    13081 atgagattga aatgataatg acaacagttg atttaaatga tcgtttaact tttcataaaa
    13141 gaaaagatcg aaagatagtt gttgagattg aacataatta tgtgccttct aatcataaaa
    13201 atctcgcata tcgtgcagcg caactattta ttgagcaata tcaactaaag caaggtgtaa
    13261 caatttctat cgataaagaa atacctgttt ctgctggctt agctggaggt tcggctgatg
    13321 cagcagcaac gttaagagga ttgaatcgac tttttgatat aggggcgagt ttggaagaat
    13381 tggctctact aggcagtaaa atcgggacag atattccgtt ttgtatttat aataaaactg
    13441 cactatgtac tggaagagga gagaaaatcg agtttttaaa taaaccacct tcagcttggg
    13501 tgattcttgc taaaccaaac ttaggcatat catcaccaga tatatttaag ttgattaatt
    13561 tagataagcg ttacgacgta catacgaaaa tgtgttatga ggccttagaa aatcgagatt
    13621 atcaacaatt atgtcaaagt ttgtctaatc gattagagcc aatttctgtt tcaaaacacc
    13681 cacaaatcga taaattaaaa aataatatgt tgaaaagtgg tgcagatggt gcgttaatga
    13741 gtggaagcgg acctactgtg tatgggctag cacgaaaaga aagccaagca aaaaatattt
    13801 ataatgcagt taacggttgt tgtaatgaag tgtacttagt tagactatta ggatagaagg
    13861 gttgaaaaga tgagatataa acgaagcgag agaattgttt ttatgacgca atatttgatg
    13921 aaccatccga ataaattgat tccattaact ttttttgtga aaaaatttaa acaggcgaag
    13981 tcttcaataa gtgaagatgt ccaaattata aaaaatacat tccaaaaaga aaagttaggt
    14041 acagtaatta ctactgctgg cgcaagtggt ggtgttacgt ataaaccaat gatgagtaaa
    14101 gaagaggcga ctgaagttgt taatgaggtc attactctat tagaagagaa agaacgtttg
    14161 ttacctggcg gatatttatt tttatcagat ttggtaggta atccatcgct actaaacaaa
    14221 gttggtaagt taattgccag tatttacatg gaagaaaaat tagatgctgt tgttaccatt
    14281 gcgacaaaag gtatttcatt ggcaaatgcg gttgctaata ttttaaattt accagtagta
    14341 gtgattagaa aagacaacaa ggtgactgaa ggttctacag tttcaattaa ttacgtttca
    14401 ggatcttcaa gaaaaataga aacaatggta ctttcgaaga gaactttagc agaaaattca
    14461 aatgttttag ttgtcgatga ttttatgagg gctggtggct ctattaatgg tgttatgaat
    14521 ttaatgaatg agtttaaagc ccatgtaaaa ggggtatcag tacttgtaga atcaaaagaa
    14581 gttaaacaaa gattgattga agattatact tccttagtga aattatctga tgtagatgaa
    14641 tataatcaag agtttaacgt agaacctggc aacagtttat ctaagttttc ataaaaggag
    14701 ttttagtatt atgaaaatca ttaacacaac aagattaccg gaagcacttg gaccatattc
    14761 gcatgcaaca gttgtgaatg gtatggttta tacttctggt cagattccat tgaatattga
    14821 tggacatatc gtaagcgctg atgttcaagc acagacaaaa caagttttag aaaatttaaa
    14881 ggttgttttg gaagaagcag gatctgattt gaattctgtt gcgaaagcga ccattttcat
    14941 taaagatatg aatgatttcc aaaaaataaa tgaagtgtat ggtcaatatt ttaatgaaca
    15001 caagccagcg cgtagttgtg tagaggttgc gcgtttgcca aaagatgtga aagtagaaat
    15061 tgaattagta agtaaaatta aggaattata attttcgatt aatatgttta atcaagcttc
    15121 taaataaaac agagagatat atactatagg ggggctcact acatgaaagt gacagatgta
    15181 agacttagaa aaatacaaac agatggacga atgaaagcac tcgtttccat tacattagat
    15241 gaagctttcg taattcatga tttacgtgta attgaaggaa actctggctt gttcgttgca
    15301 atgccaagta aacgtacacc agatggtgaa ttccgcgaca tcgcgcatcc tattaattca
    15361 gatatgagac aagaaattca agatgcagtg atgaaagtat atgatgaaac agatgaagta
    15421 gtaccagata aaaacgctac atcagaagat tcagaagaag cttaatcaat tttatattta
    15481 gcgatgtaat acatttgcaa taagttgatt tgatactgtc gataaagcat aaagctttgt
    15541 cggcagtttt tttagtttgt attaatgttt ttttattttt aatgaaaggc taataaatat
    15601 atacgttaac agattatgat gatatgaaaa ttattgattc attgctgata aaaattgcgt
    15661 ttgaatgatg ctcgtatttt tgaagtaaga aaaaagttgt ttttaaaatt acaacgaatt
    15721 aaaaacaatg ccttttatat gttgaaagag tattgcagat taaattataa taatgacgaa
    15781 gcgtaaaatt taatgggggt taatgttcat gcgaagacac gcgataattt tggcagcagg
    15841 taaaggcaca agaatgaaat ctaaaaagta taaagtgcta cacgaggttg ctgggaaacc
    15901 tatggtcgaa catgtattgg aaagtgtgaa aggctctggt gtcgatcaag ttgtaaccat
    15961 cgtaggacat ggtgctgaaa gtgtaaaagg acatttaggc gagcgttctt tatacagttt
    16021 tcaagaggaa caactcggta ctgcgcatgc agtgcaaatg gcgaaatcac acttagaaga
    16081 caaggaaggt acgacaatcg ttgtatgtgg tgacacaccg ctcatcacaa aggaaacatt
    16141 agaaacattg attgcgcatc acgaggatgc taatgctcaa gcaactgtat tatctgcatc
    16201 gattcaacaa ccatatggat acggaagaat cgttcgaaat gcgtcaggtc gtttagaacg
    16261 catagttgaa gagaaagatg caacgcaagc tgaaaaggat attaatgaaa ttagttcagg
    16321 tatttttgcg tttaataata aaacgttgtt tgaaaaatta acacaagtga aaaatgataa
    16381 tgcgcaaggt gaatattacc tccctgatgt attgtcgtta attttaaatg atggcggcat
    16441 cgtagaagtc tatcgtacca atgatgttga agaaatcatg ggtgtaaatg atcgtgtaat
    16501 gcttagtcag gctgagaagg cgatgcaacg tcgtacgaat cattatcaca tgctaaatgg
    16561 tgtgacaatc atcgatcctg acagcactta tattggtcca gacgttacaa ttggtagtga
    16621 tacagtcatt gaaccaggcg tacgaattaa tggtcgtaca gaaattggcg aagatgttgt
    16681 tattggtcag tactctgaaa ttaacaatag tacgattgaa aatggtgcat gtattcaaca
    16741 gtctgttgtt aatgatgcta gcgtaggagc gaatactaag gtcggaccgt ttgcgcaatt
    16801 gagaccaggc gcgcaattag gtgcagatgt taaggttgga aattttgtag aaattaaaaa
    16861 agcagatctt aaagatggtg ccaaggtttc acatttaagt tatattggcg atgctgtaat
    16921 tggcgaacgt actaatattg gttgcggaac gattacagtt aactatgatg gtgaaaataa
    16981 atttaaaact atcgtcggca aagattcatt tgtaggttgc aatgttaatt tagtagcacc
    17041 tgtaacaatt ggtgatgatg tattggtggc agctggttcc acaatcacag atgacgtacc
    17101 aaatgacagt ttagctgtgg caagagcaag acaaacaaca aaagaaggat ataggaaata
    17161 atcatttacg tatttaaaat ggctaggata aaaggataat cctatgtaat attaatgtaa
    17221 tctttatgat ttaatgattc gcatagtaat ggagttacat tttatatata atagtaattg
    17281 cgtaagtaaa taattggagg actataaatg ttaaataatg aatataagaa ttcgtcatta
    17341 aagatttttt cattgaaagg aaacgaagca ttagcgcaag aagttgctga ccaagtagga
    17401 attgaactag gtaaatgttc agttaaacgt tttagtgatg gagaaattca aattaatatc
    17461 gaagagagta ttcgtggttg tgacgtattt attattcaac caacatcata tcctgtgaat
    17521 ctacatttaa tggaattatt aattatgatt gatgcttgta aacgtgcttc tgcagcaaca
    17581 atcaatattg tagtgccata ttatggatat gcaagacaag atagaaaagc ccgtagccgt
    17641 gagccaatca ctgctaaatt agttgcaaac ttaatcgaaa cagctggcgc aactcgtatg
    17701 attgcgttag acttacatgc accacaaatt caaggattct ttgatattcc aattgaccac
    17761 ttaatgggtg tgccaattct tgctaaacat ttcaaagatg atccgaatat taacccagaa
    17821 gaatgtgtcg ttgtttcacc agaccatggc ggcgttacac gtgcacgtaa attagctgac
    17881 attttaaaaa ctccaattgc aattatagat aaacgtcgtc ctagaccaaa tgttgctgaa
    17941 gtgatgaaca ttgttggtga gattgaagga cgtacggcaa ttattattga cgatattatt
    18001 gatacagcag gtacaatcac tttagctgca caagcattaa aagataaagg tgctaaagaa
    18061 gtatatgctt gttgtacaca ccctgtttta tcaggaccgg ctaaagaacg tatcgaaaat
    18121 tctgctataa aagaattaat cgtaacaaac tcaattcatt tagatgaaga tcgcaaacca
    18181 tctaacacta aagaattatc tgttgctggt ttaatcgcac aagctatcat tcgtgtatac
    18241 gaaagagaat cagttagcgt attatttgac taatatttaa aaggcgtttg acgaacatat
    18301 tccaaacgtg tataatagtt tcgttcgtga ttatacgaat aaataaacac ttgcaagcaa
    18361 cgatgatgtt gatgggtaag tgaggtgctc gttttgagca aaaatgaaag gtggaaatga
    18421 gaatggcttc attaaagtca atcatccgtc aaggtaaaca aacacgttca gatcttaaac
    18481 aattaagaaa atctggtaaa gtaccagcag tagtatacgg ttacggtact aaaaacgtgt
    18541 cagttaaagt tgatgaagta gaattcatca aagttatccg tgaagtaggt cgtaacggtg
    18601 ttatcgaatt aggcgttggt tctaaaacta tcaaagttat ggttgcagac taccaattcg
    18661 atccacttaa aaaccaaatt actcacattg acttcttagc aatcaatatg agtgaagaac
    18721 gtactgttga agtaccagtt caattagttg gtgaagcagt aggcgctaaa gaaggcggcg
    18781 tagttgaaca accattattc aacttagaag taactgctac tccagacaat attccagaag
    18841 caatcgaagt agacattact gaattaaaca ttaacgacag cttaactgtt gctgatgtta
    18901 aagtaactgg cgacttcaaa atcgaaaacg attcagctga atcagtagta acagtagttg
    18961 ctccaactga agaaccaact gaagaagaaa tcgaagctat ggaaggcgaa caacaaactg
    19021 aagaaccaga agttgttggc gaaagcaaag aagacgaaga aaaaactgaa gagtaatttt
    19081 aatctgttac attaaagttt ttatactttg tttaacaagc actgtgctta ttttaatata
    19141 agcatggtgc tttttgtgtt attataaagc ttaattaaac tttattactt tgtactaaag
    19201 tttaattaat tttagtgagt aaaagacatt aaactcaaca atgatacatc ataaaaattt
    19261 taatgtactc gattttaaaa tacatactta ctaagctaaa gaataatgat aattgatggc
    19321 aatggcggaa aatggatgtt gtcattataa taataaatga aacaattatg ttggaggtaa
    19381 acacgcatga aatgtattgt aggtctaggt aatataggta aacgttttga acttacaaga
    19441 cataatatcg gctttgaagt cgttgattat attttagaga aaaataattt ttcattagat
    19501 aaacaaaagt ttaaaggtgc atatacaatt gaacgaatga acggcgataa agtgttattt
    19561 atcgaaccaa tgacaatgat gaatttgtca ggtgaagcag ttgcaccgat tatggattat
    19621 tacaatgtta atccagaaga tttaattgtc ttatatgatg atttagattt agaacaagga
    19681 caagttcgct taagacaaaa aggaagtgcg ggcggtcaca atggtatgaa atcaattatt
    19741 aaaatgcttg gtacagacca atttaaacgt attcgtattg gtgtgggaag accaacgaat
    19801 ggtatgacgg tacctgatta tgttttacaa cgcttttcaa atgatgaaat ggtaacgatg
    19861 gaaaaagtta tcgaacacgc agcacgcgca attgaaaagt ttgttgaaac atcacgattt
    19921 gaccatgtta tgaatgaatt taatggtgaa gtgaaataat gacaatattg acaacgctta
    19981 taaaagaaga taatcatttt caagacctta atcaggtatt tggacaagca aacacactag
    20041 taactggtct ttccccgtca gctaaagtga cgatgattgc tgaaaaatat gcacaaagta
    20101 atcaacagtt attattaatt accaataatt tataccaagc agataaatta gaaacagatt
    20161 tacttcaatt tatagatgct gaagaattgt ataagtatcc tgtgcaagat attatgaccg
    20221 aagagttttc aacacaaagc cctcaactga tgagtgaacg tattagaact ttaactgcgt
    20281 tagctcaagg taagaaaggg ttatttatcg ttcctttaaa tggtttgaaa aagtggttaa
    20341 ctcctgttga aatgtggcaa aatcaccaaa tgacattgcg tgttggtgag gatatcgatg
    20401 tggaccaatt tcttaacaaa ttagttaata tggggtacaa acgggaatcc gtggtatcgc
    20461 atattggtga attctcattg cgaggaggta ttatcgatat ctttccgcta attggggaac
    20521 caatcagaat tgagctattt gataccgaaa ttgattctat tcgggatttt gatgttgaaa
    20581 cgcagcgttc caaagataat gttgaagaag tcgatatcac aactgcaagt gattatatca
    20641 ttactgaaga agtgatcagc catcttaaag aagagttaaa aactgcatat gaaaatacaa
    20701 gacccaaaat agataaatca gtgcgcaatg atttgaaaga aacgtatgaa agctttaaat
    20761 tattcgaaag tacatacttt gatcatcaaa tactacgtcg cttagtagcg tttatgtatg
    20821 aaacaccttc gacaattatt gagtatttcc aaaaagatgc aatcattgca gttgatgaat
    20881 ttaatcgtat taaagaaact gaagaaagtt taacagtaga gtctgattcg tttattagca
    20941 atattattga aagtggtaat ggatttatag gacaaagttt tataaaatat gatgattttg
    21001 aaacattgat tgaaggctat cctgtcactt atttttcatt attcgctaca acaatgccga
    21061 taaaactaaa tcatattatt aaattttcat gtaaacctgt ccaacaattt tatgggcaat
    21121 atgacattat gcgttctgaa tttcaacgat atgttaatca aaactatcat atcgtggttt
    21181 tggtcgaaac cgaaactaaa gttgaacgta tgcaagcgat gttaagtgaa atgcatattc
    21241 catcaataac aaaattgcat cgctcaatgt catcggggca agcagtgatt attgaaggca
    21301 gtttatctga aggatttgaa ctacctgata tgggattagt tgtcattact gagcgtgagc
    21361 tttttaaatc aaaacagaaa aagcaacgaa aacgtacgaa agctatctca aatgctgaaa
    21421 aaattaagtc ttaccaagat ttaaatgtgg gagattatat tgttcatgtg catcatggtg
    21481 ttggtagata tttaggtgtt gagacgctcg aagtggggca aacgcatcgt gattatatta
    21541 aattgcaata taaaggtacg gatcaactat ttgttccagt agatcaaatg gatcaagttc
    21601 aaaaatatgt agcttcggaa gataagacgc caaaattaaa taaactcggt ggcagtgaat
    21661 ggaaaaaaac aaaagctaaa gttcaacaaa gtgttgaaga tattgctgaa gagttgattg
    21721 atttatataa agaaagagaa atggcagaag gttatcaata tggggaagac acagctgagc
    21781 aaacaacatt tgaattagat tttccatatg aacttacgcc tgaccaagct aaatctatcg
    21841 atgaaattaa agatgacatg caaaaatcgc gtccaatgga tcgcttgcta tgtggtgatg
    21901 ttggttatgg taaaactgaa gttgcagtga gagcagcatt caaagctgta atggaaggaa
    21961 agcaggttgc atttttagtt cctacaacta ttttagctca gcaacattat gagacgttaa
    22021 ttgagcgtat gcaagatttt cctgttgaaa ttcaattaat gagtcgtttt agaacgccta
    22081 aagagataaa acaaactaag gaaggactta aaactggatt tgttgacata gttgttggta
    22141 cacacaaatt acttagtaaa gatatacagt ataaagattt agggctgttg attgtagatg
    22201 aagaacaacg atttggtgta cgccataaag agcgtattaa aacattaaaa cataatgtag
    22261 atgtactaac attgactgca accccaatac ctagaacatt gcatatgagt atgctaggtg
    22321 tgcgggattt gtcagtgatt gaaacgccgc cagaaaatcg tttcccagtt caaacatatg
    22381 tattagaaca gaacatgagt tttatcaaag aagctttaga aagagaacta tcccgtgatg
    22441 gccaagtgtt ttatctttat aataaagtgc aatccattta tgaaaaacga gaacaactcc
    22501 agatgttaat gccagatgct aacattgcag ttgctcatgg acaaatgaca gagcgcgatt
    22561 tagaagaaac gatgttaagt tttatcaata atgaatatga tattttagta acgacgacga
    22621 ttattgaaac aggtgtcgat gtcccaaatg caaatacttt gatcattgaa gatgcagatc
    22681 gctttggatt gagtcagttg tatcaattaa gaggtcgtgt tggtcgttca agtcgtattg
    22741 gttatgcata cttcttacat ccagcaaata aggtactaac tgagactgca gaagatcgat
    22801 tacaagcgat taaagaattt acggagttag gctcaggatt taagattgcg atgcgtgatt
    22861 tgaacattcg tggtgctggt aatttgttag gtaaacaaca gcacggcttt attgatacag
    22921 ttggatttga tttgtacagt caaatgttag aagaagctgt aaatgaaaaa cgtggtatta
    22981 aggaaccaga atctgaggtg ccagaagtcg aagttgattt aaacttggat gcatatttgc
    23041 caacagaata tattgcaaat gaacaagcta aaattgaaat ttataaaaag ctacgaaaaa
    23101 ctgaaacatt tgatcaaatt atcgacatta aagatgaatt aattgatcgt ttcaatgatt
    23161 atcctgttga agtagcacgt ttgcttgata tagtggaaat aaaagtacac gcattacatt
    23221 caggtatcac gttgattaaa gataaaggga aaataattga tattcattta tctgtaaaag
    23281 ccactgaaaa tattgatggc gaagtgctgt tcaaagcaac acaaccttta ggtagaacaa
    23341 tgaaggttgg tgttcaaaat aatgcaatga caattacttt aacgaaacaa aatcaatggc
    23401 ttgatagttt gaagttttta gttaagtgca ttgaagaaag tatgagaatc agtgatgaag
    23461 cataaagaag catttaatgg cgttgtcgtg ttaactgctg cattaattgt cattaaaatt
    23521 ctgagtgctg tatatcgaat tccatatcaa aatatattag gcgatacagg tttgtatgca
    23581 tatcaacaag tgtatccaat tgtagcatta ggaatgatat tatcgatgaa tgccattcct
    23641 agtgcaatta cacaaaatat agggaagtat catagtgacg aagcatatgc aaaagcagtc
    23701 gcttatatac aattagttgg tatattatta tttattgcta tttttgtgtt tgcgaacaat
    23761 attgcacata tgatgggtga tggccattta acaccaatga ttcaagctgc aagtttaagc
    23821 tttatattta taggtatgct tggcgtgtta agaggttatt atcaatctgc aaataatatg
    23881 acagttccgg ctatttccca ggttatagaa caagttatac gagtaggtat tatcattgtt
    23941 actattgtta tttttgtaga cagaggttgg acgatatatg aagcgggaac aattgctatt
    24001 ttagcatcaa cgataggttt tttaggttct tcaatttatt tagtagcgca ccgacctttt
    24061 aagtttaaaa tggtaaataa cactgcaaag atcgtttgga aacagttcgc actttcggtt
    24121 ttgattttcg ctatcagtca attaatcgta attttatggc aagtgattga tagtgttact
    24181 attattaagt cacttcaagc gatacgcgtg ccattcgatg ttgccataac tgaaaaagga
    24241 gtctatgacc gtggtgcatc atttattcag atgggattga ttgtaactac aacatttagt
    24301 tttgcgctca ttcctctgtt aagtgacgca attaaaatga ataatcaggt acttatgaat
    24361 cgttatgcaa atgcgtcatt aaagattacg attttaataa gtacagcagc aggaatagga
    24421 ttaattaatt tattgccttt aatgaacggt gtgtttttta agacgaatga tttaacctta
    24481 acgttaagtg tttatatgat tacggtcatt tgtgtatcgt taattatgat ggatatggca
    24541 ttattacaag cgcaacatgc tgtgagacct atttttgttg gtatgacggc aggattggtt
    24601 attaaattta tacttaatat cattttgatt cgtttaagtg gcattattgg tgcgagcatt
    24661 agtactgttg tatcattaat tatattcggt acgattatcc atattgctgt cacgagaaaa
    24721 taccacttat atgcgatgag acgatttttt atcaatgttg ttttaggtat ggtatttatg
    24781 tcgattgttg ttcaatgcgt gttaaacata gtgacaacac acggtagaat cactggactc
    24841 attgaattat tatgtgcagc agtattaggt atcattgcat tgtttttcta tatttttaga
    24901 tttaatgttt tgacatataa agagttaact tatttaccat ttggttcaaa gttgtatcaa
    24961 attaagaaag gaagacgttg atggcacata ccattacgat tgttggctta ggaaactatg
    25021 gcattgatga tttgccgcta gggatatata aatttttaaa gacacaagat aaagtttatg
    25081 caagaacgtt agatcatcca gttatagaat cattgcaaga tgaattaaca tttcagagtt
    25141 ttgaccatgt ttatgaagca cataaccaat ttgaagatgt ctatattgat attgtggcgc
    25201 aattggttga agctgctaat gaaaaagata ttgtctatgc ggttccgggt catcctagag
    25261 ttgctgagac aactacagtg aaattactgg ctttagcaaa ggacaatact gatatagatg
    25321 tgaaagtttt aggtgggaaa agctttattg atgatgtgtt tgaagcagtt aatgtagatc
    25381 caaatgatgg cttcacactg ttagatgcga catcattaca agaagtaaca cttaatgtta
    25441 gaacgcatac attgattacg caagtttata gtgcaatggt tgctgctaat ttgaaaatca
    25501 ctttaatgga acgatatcct gatgattacc ctgttcaaat tgtcactggt gcacgaagcg
    25561 atggtgcgga taacgttgtg acatgcccat tatatgaatt ggatcatgat gaaaatgcat
    25621 tcaataattt gacgagtgta ttcgtaccaa aaatcataac atcgacatat ttgtatcatg
    25681 actttgattt tgcaacggaa gtgattgata ctttagttga tgaagataaa ggttgtccat
    25741 gggataaagt gcaaacgcat gaaacgctaa agcgttattt acttgaagaa acatttgaat
    25801 tgttcgaagc tattgacaat gaagatgatt ggcatatgat tgaagaacta ggagatattt
    25861 tattacaagt gttattgcat actagtattg gtaaaaaaga agggtatatc gacattaaag
    25921 aagtgattac aagtcttaat gctaaaatga ttcgtagaca cccacacata tttggtgatg
    25981 ccaatgctga aactatcgat gacttaaaag aaatttggtc taaggcgaaa gatgctgaag
    26041 gtaaacagcc aagagttata tttgaaaaag tatttgcaga gcatttttta aatttatatg
    26101 agaagacgaa ggataagtca tttgatgagg ccgcgttaaa gcagtggcta gaaaaagggg
    26161 agagtaatac atgagattag ataaatattt aaaagtatca cggttaataa agcgacgtac
    26221 gctagcaaaa gaagtaagtg atcaaggtag aattacaata aatggtaatg ttgctaaagc
    26281 tggatcggat gttaaagttg aagatgtgct gacgattcgc tttggtcaaa aattagtaac
    26341 agttaaagta actgcattaa atgaacatgc atctaaagat aacgcgaagg gtatgtatga
    26401 aatcattgaa gagcgtcgac ttgaagaagc gtaaattgga ggtgacaagc aatgaaaaat
    26461 aaagtagaac atatagaaaa tcagtacacg tcgcaagaga acaagaaaaa acaacgtcaa
    26521 aaaatgaaaa tgcgtgttgt tcgtaggcgt attacagtat ttgcgggcgt attacttgcg
    26581 ataattgttg ttttatcaat cttgcttgtt gtccaaaaac atcgcaatga tattgatgca
    26641 caggagcgaa aagcgaaaga agcacagttt caaaagcaac aaaatgaaga aattgcgtta
    26701 aaagaaaagt tgaataatct gaatgacaaa gattacattg aaaaaattgc gcgtgatgat
    26761 tattacttaa gcaacaaagg tgaagtgatt tttaggttgc cagaagacaa agattcgtct
    26821 agctcaaaat cttcgaaaaa ataaatccaa attgattcaa aattatccga gtatagacat
    26881 tgtgaaaaaa tccaaacaag gatataataa gggaaaatcg aatcaaatcg ggaggattta
    26941 tttaacatat gtcaatcgaa gttggaaata agcttaaagg taaagtcact ggtattaaaa
    27001 agtttggtgc attcgtagaa ttacctgaag gaaaaagtgg tttagttcac attagtgaag
    27061 tcgcagataa ttatgttgaa aacgtagaag agcacctttc tgttggtgat gaagtagacg
    27121 taaaagtatt atctattgct gatgatggaa aaattagtct ttcaattaag aaagctaaag
    27181 accgtccacg tagacaacat acgagtaaac caagtcatca aaaaccagtg caaaaagccg
    27241 aagattttga aaagaaatta agcaatttct taaaagatag tgaagataaa ttaacttcaa
    27301 tcaaacgtca aacagaatct agacgcggtg gcaaaggttc aagacgttaa ttaaaataaa
    27361 taaagactgc ttcgataagg aatatattta gaatgatgcg tatcgaataa tcgattgcag
    27421 cgttagacaa tctaagactg tttcttaaat aaggagcagt ctcttttatt tgtaatgata
    27481 taactaagac ttataccatt tttgaaaatt gtaaaagtga ggtgatgtta tgcagttaaa
    27541 tagtaatggt tggcatgttg atgaccatat tgttgtcgct gtttctacag gtattgatag
    27601 tatgtgttta ttgtatcaac tactaaatga ttataaagat agttatagaa aactaacatg
    27661 cttacatgtc aatcatggcg ttaggtcagc ttcaattgag gaagccagat ttttagaagc
    27721 atactgcgaa cgtcatcaca tcgatttaca tatcaaaaag ttagatttgt cgcatagtct
    27781 cgaccgaaat aacagcattc agaatgaagc tcgaattaaa cgttacgaat ggtttgatga
    27841 aatgatgaat gtattagaag cggatgtatt gctaacggcg catcatttgg acgatcaatt
    27901 agaaactatt atgtatcgta tttttaatgg gaaatcaaca cgtaataaac taggatttga
    27961 tgagttatcg aagcgaaaag gttatcagat ttatcgacca cttttagctg tctctaaaaa
    28021 agaaataaaa caattccaag agagatatca tattccatat tttgaagatg aatctaataa
    28081 agataacaaa tatgttagaa atgatattcg taatagaatt attccagcta ttgatgaaaa
    28141 taatcaactt aaagtatcgc atttattaaa attaaaacaa tggcatgatg aacaatatga
    28201 tattttgcaa tattcagcta aacaatttat tcaagaattt gtgaagtttg atgaacagtc
    28261 aaaatattta gaggtttcta gacaagcttt taataactta ccaaactcat taaagatggt
    28321 tgtgttggac tgcctattat caaagtatta tgagttgttt aatattagtg ctaaaacata
    28381 cgaagagtgg tttaaacaat ttagtagtaa gaaagcacaa ttcagtatta atctcacgga
    28441 taaatggata attcaaatcg catatggtaa attaataata atggctaaaa ataatggcga
    28501 tacatatttt agagttcaaa caattaaaaa gccaggtaat tatattttta acaaatatcg
    28561 attagagata cattctaatt taccaaaatg tttatttccg cttacagtga gaacacgaca
    28621 aagtggcgat acatttaaac tgaatgggcg cgatggttat aagaaagtga atcgcctgtt
    28681 tatagattgt aaagtgccac agtgggttcg ggatcaaatg ccaatcgtat tggataaaca
    28741 acagcgcatt attgcggtag gagatttata tcaacaacaa acaataaaaa aatggattat
    28801 aattagtaaa aatggagatg aatagcgtta tgcataatga tttgaaagaa gtattgttaa
    28861 ctgaagaaga tattcaaaat atctgtaagg aattgggagc acaattaaca aaggattatc
    28921 aaggtaaacc attagtatgc gtgggtatct taaaaggctc agcaatgttt atgtcagatt
    28981 taattaaacg aattgatacc catttatcaa ttgatttcat ggatgtttct agttatcacg
    29041 gaggcactga gtcaactggt gaagttcaaa tcattaaaga tttaggttct tctattgaaa
    29101 ataaagacgt attaattatt gaagatatct tagagactgg tactacactt aagtcaatta
    29161 ctgaattatt acaatctaga aaagttaatt cattagaaat agttacttta ttagataaac
    29221 caaaccgtcg taaagcggac attgaagcta agtatgtagg taaaaaaata ccagatgaat
    29281 ttgttgttgg ttacggttta gattatcgtg aattataccg aaacttacca tatatcggta
    29341 cgttaaaacc tgaagtgtat tcaaattaat tttttaatca atttcagtta ttattactat
    29401 gcgtttgaga aataatagtg tagactcaaa aatatgaaaa atgtatttca tatatattta
    29461 attttagaca agacatatgt cttgaaaagt tgaaaaatat agagattgat aaaactaata
    29521 cgggtgtgaa tgacattgat gttaagctca attactagct tataaaacat gtcatatgtt
    29581 acaatttttg ttagttttat tatgggaagt aggaggaaat gacgcatgca gaaagctttt
    29641 cgcaatgtgc tagttatcgt aataataggc gttattattt ttggtctatt ttcatattta
    29701 aacggtaatg gaaatatgcc gaaacagctt acatataatc aatttactga gaagttggaa
    29761 aaaggtgacc ttaaaacttt agaaatccaa ccacaacaaa atgtctatat ggtaagtggt
    29821 aaaacgaaaa atgatgaaga ctattcatca actattttat ataacaacga aaaagaatta
    29881 caaaaaatta ctgatgctgc taaaaagcaa aacggtgtaa aattaacgat taaagaagaa
    29941 gaaaaacaaa gtgtctttgt gagtatactt tcaacattaa ttccagttgt agtcatagcg
    30001 ttattattta ttttcttcct aagccaagca caaggtggcg gtagtggcgg tcgtatgatg
    30061 aactttggta aatctaaagc aaaaatgtac gataataata aacgtcgtgt tcgtttctct
    30121 gatgtagcag gggcagatga agaaaaacaa gaattaattg aaattgttga tttcttgaaa
    30181 gataataaaa aattcaaaga aatgggatct aggattccta aaggtgtctt acttgttgga
    30241 cctccaggta ctggtaaaac attacttgct agagcggttg caggtgaagc tggcgcacca
    30301 ttcttctcta ttagtggttc agactttgta gagatgtttg ttggtgttgg tgcgagccgt
    30361 gttcgtgact tattcgataa tgctaagaaa aacgcgcctt gtatcatctt tatcgatgag
    30421 attgatgctg ttggtcgtca acgtggtgca ggtgttggtg gcggtcatga tgaacgtgaa
    30481 caaaccctaa accaattatt agttgaaatg gatggtttcg gtgaaaatga aggtatcatt
    30541 atgatagctg ctacaaaccg tcctgatatc cttgacccag ccttattacg tccaggtcgt
    30601 tttgatagac aaattcaagt tggtcgtcca gatgtgaaag gccgtgaagc aattcttcat
    30661 gttcatgcta aaaacaaacc acttgatgaa acggttgatt taaaagcaat ttcacaacgt
    30721 acacctggtt tctcaggtgc tgatttagag aacttattaa atgaagcatc tttaattgct
    30781 gtacgtgaag gtaaaaagaa aattgacatg agagatatcg aagaggcaac ggatagagtt
    30841 atagccggac ctgctaagaa atctcgagtt atttctaaga aagaacgtaa tattgttgct
    30901 catcacgaag ctggtcatac aattatcggt atggtacttg atgaggcaga agtagtgcat
    30961 aaagttacta ttgttccacg tggacaagca ggtggttatg caatgatgct acctaaacaa
    31021 gatcgtttct taatgactga acaagagtta ttagataaaa tctgtggttt acttggtgga
    31081 cgtgtatcag aagatattaa ctttaacgaa gtatcaacag gtgcttcaaa tgacttcgaa
    31141 cgtgcaacac aaatcgcacg ctcaatggtt acgcaatatg gtatgagtaa aaaattagga
    31201 ccattacagt tcggtcatag caatggtcaa gtattcttag gtaaagatat gcaaggtgag
    31261 cctaattatt caagccaaat cgcatatgaa attgataaag aagttcaacg aatcgttaaa
    31321 gaacaatacg aacgttgtaa acaaatttta ttagagcaca aagaacaatt aattttaatt
    31381 gctgaaacat tattaacaga agaaacatta gttgctgaac aaattcaatc attattctac
    31441 gaaggtaaat tacctgaaat tgattatgat gcagctaaag ttgttaaaga tgaagattct
    31501 gaatttaatg atggtaaatt cggtaaatct tatgaagaga ttcgtaaaga gcaattagaa
    31561 gatggacaac gtgacgaaag tgaagatcgt aaagaagaaa aagatattgc tgaggataaa
    31621 aaagaagctg ataaatctga tgaaaaagat gaaccagcac atcaacaagc cccaaatatc
    31681 gaaaaacctt acgatccaaa tcacccagac aataaataat cgattatatt cagtacctct
    31741 ttctatgata aagttataga aagaggtact tttatcgttt ttgaaaatac gtattagatt
    31801 ttaagtcgtt gaattgttat agcagaaaat aattgtaaaa caagttactt cattatttag
    31861 aatgatgggt gtagaataag tacaattgtt gcattttatg aagtaaagta attttttaaa
    31921 tatagagtaa tagaggagat tgaaataatg acacacgatt atattgttaa agcattagca
    31981 tttgatggag agattagggc ttatgctgct ttgacaactg aaactgttca agaagcacaa
    32041 acgagacatt atacatggcc gacagcatct gctgcaatgg gaagaacaat gacagcaaca
    32101 gctatgatgg gcgcaatgtt gaaaggtgat caaaaattaa ctgtcactgt agatggccaa
    32161 ggacctattg gacgaattat tgccgatgca aatgctaaag gcgaggtgcg tgcttatgta
    32221 gaccatccac aaactcattt tccattaaat gagcaaggta aacttgatgt aagacgagcg
    32281 gtagggacaa atggatctat tatggttgtt aaagacgttg gaatgaaaga ctatttctct
    32341 ggagcaagtc caattgtttc aggagaactt ggtgaagatt ttacttatta ttatgctaca
    32401 agtgaacaaa caccttcatc ggtaggtctt ggtgtattgg taaatcctga taatacgatt
    32461 aaagcagcag gaggatttat cattcaagtt atgccaggtg ccaaagatga aacaatttca
    32521 aaattagaaa aagcaattag tgaaatgaca ccagtttcta aattaattga acaaggatta
    32581 acgccagaag gattactaaa cgaaatctta ggtgaagacc atgtgcaaat tttagagaaa
    32641 atgcctgttc aatttgaatg taattgtagt catgagaaat ttttaaatgc tattaaagga
    32701 ttgggcgagg ctgagattca aaatatgatt aaagaagatc atggtgctga agcagtatgt
    32761 catttctgtg gaaataaata taaatatact gaagaagaat taaacgtgtt gctagaaagt
    32821 ttagcgtaat ttaatttaaa tcaatacgct aaaatgttta tttttagcgg tttagtgaaa
    32881 tgtagaacta aatagttgta taatccttag tgattttgtt tgctttctag aatttatttg
    32941 ataaaataat tctatatccg ataaataaac taagatttca acaactaact aaaaaggagt
    33001 gttcttaatg gcacaaaaac cagtagataa tattactcaa attattggcg gtacaccggt
    33061 agtcaaattg agaaatgtag tagatgacaa tgcagcagat gtttatgtaa aattggaata
    33121 tcaaaatcca ggtggttctg taaaggatag aattgcttta gcaatgattg aaaaagcaga
    33181 gcgagaaggc aaaattaaac ctggcgatac aattgtagaa ccaacaagtg gtaatacagg
    33241 tatcggttta gcatttgtat gtgctgctaa aggatataaa gcagtattta ctatgcccga
    33301 aacaatgagc caagagcgtc gtaatttatt aaaagcatac ggtgcggaat tagttttaac
    33361 gcctggatca gaagcgatga aaggtgcaat taaaaaagct aaagaattga aagaagaaca
    33421 tggttacttc gagccacaac aatttgaaaa ccctgcgaac cctgaagttc atgagttaac
    33481 tacaggtcct gagttattac aacaatttga agggaaaact atcgatgcgt tcctagctgg
    33541 tgttggtact ggtggtacgt tatctggtgt aggtaaagtt ctgaaaaaag aatatcctaa
    33601 catcgaaatt gttgctatag agcctgaggc ttctccagta ttgagcggtg gtgagccagg
    33661 tccacataaa ttacaaggtt taggtgctgg atttattcca ggcactttga atacagaaat
    33721 ctatgacagt attattaaag taggaaatga tacagcgatg gaaatgtctc gtcgagttgc
    33781 taaagaggaa ggtattttag caggtatttc atcaggtgct gcgatttatg ctgccattca
    33841 aaaagcaaaa gaattaggaa aaggtaaaac agtagtaaca gtattgccga gtaatggtga
    33901 acgctactta tcaacacctt tatattcatt cgatgactaa ttaatgtcat ttaaaagagt
    33961 gagttatctt tttgagataa cttgctcttt ttttctacca tgtatatttt taaaaatatg
    34021 agcgttaaat taaacatttt tctgataaaa atatccagtg aatgataaga taataaacgt
    34081 acatactaat aactagtaaa tagcaggagt aaattttatt agagttaaac aatacataat
    34141 taaagggtgg ttaacatgac taaaacaaaa attatgggca tattaaacgt cacacctgat
    34201 tcattctcag atggtggaaa atttaataat gttgaatcag ctataaatag agtgaaagcc
    34261 atgatagatg aaggtgctga cattatagat gttggaggtg tttcaacgag acccggtcat
    34321 gaaatggttt cattagaaga agagatgaac agagtattac ctgttgttga agctattgtc
    34381 ggttttgatg taaaaatttc agtcgataca tttcgaagtg aggttgctga agcatgttta
    34441 aaattaggcg ttgatatgat taatgatcaa tgggcgggtc tgtatgatca tcgtatgttc
    34501 caaattgtag ctaaatatga cgcggaaatt attttaatgc ataatggaaa tggtaatcgt
    34561 gatgaaccgg ttgtcgaaga aatgttaaca tctttgttag cacaagcaca tcaagctaaa
    34621 atagctggta taccttcaaa taaaatttgg ctagatccag gtataggttt cgctaaaact
    34681 agaaatgaag aagccgaagt tatggcaaga ctggatgaac ttgttgcaac agaatatcca
    34741 gttttattag cgacaagccg gaaacgtttc actaaagaga tgatgggtta tgatacaaca
    34801 ccggttgaaa gagatgaagt aactgcagct acgactgcat atggtattat gaaaggcgtt
    34861 agagcagtac gcgttcataa tgtcgagttg aatgctaaat tagctaaagg tatagatttt
    34921 ttaaaggaga atgaaaatgc aagacacaat ctttcttaaa ggtatgcgct tttatggata
    34981 tcatggtgct ttatcagctg aaaatgaaat agggcaaatt ttcaaagtgg atgtaacttt
    35041 gaaagtagac ttagctgaag ctgggcgtac tgataatgtt attgatacag ttcattatgg
    35101 tgaagtgttc gaagaggtta aatcaattat ggaaggtaag gccgttaatt tacttgagca
    35161 tctagctgaa cgtattgcaa atcgtataaa ttcacaatat aatcgtgtaa tggaaacgaa
    35221 agtgagaatc actaaagaaa acccaccgat tccgggtcat tatgatggag taggtatcga
    35281 aatagtgagg gagaataaat gattcaagca tacttaggat taggtagtaa tattggtgat
    35341 agagaaagcc agttaaacga tgctataaag attttgaatg aatatgatgg tattaacgta
    35401 tctaatattt ctccgattta tgaaacagca ccagttgggt atactgagca acctaacttt
    35461 ttaaatttgt gtgttgaaat tcaaacaaca ctcacagtat tacaactgtt ggaatgttgt
    35521 ttgaagacag aagaatgttt acaccgtatt agaaaggaac gatggggtcc tagaacttta
    35581 gatgtggata ttttgttgta tggagaagaa atgatagatt taccaaaact gtcggtgcca
    35641 catccgagaa tgaatgaacg tgcatttgtt ttaatcccat taaatgatat agcagcaaat
    35701 gtcgtagaac cacgttcgaa attgaaagtg aaagatttag tttttgtcga tgacagtgta
    35761 aagagatata aataatgtat tgttgagaac attcatattt attgggaata gattattgat
    35821 tattggagat gtttccgcat tttgattatc tctaaatgca tttgatttcg aattagtata
    35881 tgatgattta gtatgtgtta gaattaagtt atttcataaa atcaaagtaa gcaatatttg
    35941 ataaattgct gtaatttatt taggcttttg ttgaagtttg tggaagataa tttttataca
    36001 tttacttagg gttgtattaa tgataatcgt attttaaatg aataagtaat tcataaacga
    36061 aaacagaaat tgctttatta catttacaag ctcttgtaaa attaaaaaag tgataaaatt
    36121 gatgttatga atataagaaa acatgccaca tcataacttt aaggtgtaat ggttaatgat
    36181 aaagtattag aaacatcgaa agagatgtat gttgagcaaa aatgtctgat attttataaa
    36241 actttaaagg aaaatgtttg agtgtaccag ttggaatact aaaggattac aacaagttaa
    36301 aggagagaaa gttatgtcag aagaaatgaa tgaccaaatg ttggttcgac gtcaaaaatt
    36361 acaagaatta tatgatcttg gtatagaccc gtttggttct aaatttgacc gttcaggttt
    36421 atctagtgat ttgaaagaag agtgggacca gtattctaaa gaagaattgg tagaaaaaga
    36481 agcggatagt catgtcgcta tagctggacg attaatgact aagcgtggta aaggtaaagc
    36541 aggatttgca cacgttcagg acttagctgg acaaattcaa atttacgttc gtaaagatca
    36601 agttggcgat gacgaatttg atttatggaa aaatgctgat ttaggcgata tcgttggtgt
    36661 tgaaggtgta atgttcaaaa caaatactgg cgaattatcg gttaaagcga agaaattcac
    36721 gctactaact aaatcattgc gaccattacc ggataaattc cacggtttac aggatattga
    36781 acagagatat cgtcaaagat atttagattt aattacgaac gaagatagca ctcgtacatt
    36841 tattaatcgt agtaaaatca ttcaagaaat gcgtaattat ttaaataata aaggtttctt
    36901 ggaagtagaa acacctatga tgcaccaaat tgctggtgga gcagctgcta gaccatttgt
    36961 aacacatcat aatgcattag atgcaacgtt atacatgcgt attgctattg agttgcattt
    37021 aaaacgttta attgtcggtg gacttgaaaa agtatatgaa attggtagag tattccgtaa
    37081 tgaaggtgta tcaactagac ataaccctga attcacaatg attgaattat atgaagcata
    37141 tgcagattat catgacatta tggatttaac agaatctatg gtgagacata ttgccaatga
    37201 agtgttaggt tctgcaaaag tacaatacaa tggggaaacg atcgatttag aatctgcttg
    37261 gactcgtttg catattgttg atgctgtaaa agaagctact ggtgtagatt tttatgaagt
    37321 taaaagtgat gaagaagcta aagctttagc taaagaacat ggtattgaaa ttaaagatac
    37381 aatgaaatat ggtcatattt taaatgaatt ctttgagcaa aaagttgaag aaacacttat
    37441 tcagccaacg tttatctatg gtcatccgac tgaaatttca cctttagcga agaaaaatcc
    37501 tgaagatcct agatttactg atcgtttcga attgttcatt gtaggtagag agcatgcaaa
    37561 tgcatttact gaattaaatg atcctattga tcaaaaaggt cgttttgaag cgcaacttgt
    37621 tgaaaaagcg caaggtaatg atgaagcgca tgaaatggat gaagattaca ttgaagcgtt
    37681 agaatatggt atgcctccga caggtggtct tggtatcggt attgacagat tggttatgtt
    37741 attaactgac tctccatcaa tcagagacgt attattattc ccttatatga gacaaaaata
    37801 aatgacgttg attgttagta agagctctcg tgtatacaac atgtgtatgc gagggctttc
    37861 ttaattatgg taattagttc gtgtttgaat gtttttgata gtaatagtta acgatagtgg
    37921 tgctattttt gactgttaaa caaggtagtt ggctgataga tgaaaatgac gatacgtata
    37981 tagagtagtt cggatgagaa atgttaacga tagacattga gatatcttat aacaaaaatt
    38041 gctatattag tataatttat cttaatcagc tataaaagta ctttaaaatt gtatagaatg
    38101 tgtatggttt tgtacatatg tgtatgatag aatactaaaa gtgttatgaa ttagagggca
    38161 cgagaaatgt cagttttgaa gaataaaaaa gttgattaaa agtgttgact ttatcaattg
    38221 aatgaagtaa tatataaaag tcgccaaaaa cagacgaaac acactaaaag ctgatgtgac
    38281 aaagtttaca tcaaagtgta aaattaacta ttgcacctta ttaattaagc gtgtatcatg
    38341 aataagtaag ttattttgtc tggtgactat agcaaggagg tcacacctgt tcccatgccg
    38401 aacacagaag ttaagctcct tagcgtcgat ggtagtcgaa cttacgttcc gccagagtag
    38461 aacgttgcca ggcaaatgac aaatcggaga attagctcag ctgggagagc atctgcctta
    38521 caagcagagg gtcggcggtt cgaacccgtc attctccacc atttattctt agatatagcc
    38581 ggcctagctc aattggtaga gcaactgact tgtaatcagt aggttggggg ttcaagtcct
    38641 ctggccggca ccatcttttg agccattagc tcagctggta gagcatctga cttttaatca
    38701 gagggtcaga ggttcgaatc ctctatggct cattacgatt taatttttat atttagcaaa
    38761 ataatgcaga agtagttcag cggtagaata caaccttgcc aaggttgggg tcgcgggttc
    38821 gaatcccgtc ttctgctcca ttattttgcc ggggtggcgg aactggcaga cgcacaggac
    38881 ttaaaatcct gcggtgagag atcaccgtac cggttcgatt ccggtcctcg gcaccatttt
    38941 agcgcccgta gctcaattgg atagagcgtt tgactacgga tcaagaggtt atgggttcga
    39001 ctcctatcgg gcgcgccatt tttaaattaa ttgaataacg ggaagtagct cagcttggta
    39061 gagcacttgg tttgggacca aggggtcgca ggttcgaatc ctgtcttccc gattacttct
    39121 taaattccat tttatggggg cttagctcag ctgggagagc gcctgctttg cacgcaggag
    39181 gtcagcggtt cgatcccgct agtctccacc atttattttt tacacgatga acattgaaaa
    39241 ctgaatgaca atatgtcaac gttaattcca aaaaacgtaa ctataagtta caaacattat
    39301 ttagtattta tgagctaatc aaacatcata atttttatgg agagtttgat cctggctcag
    39361 gatgaacgct ggcggcgtgc ctaatacatg caagtcgagc gaacggacga gaagcttgct
    39421 tctctgatgt tagcggcgga cgggtgagta acacgtggat aacctaccta taagactggg
    39481 ataacttcgg gaaaccggag ctaataccgg ataatatttt gaaccgcatg gttcaaaagt
    39541 gaaagacggt cttgctgtca cttatagatg gatccgcgct gcattagcta gttggtaagg
    39601 taacggctta ccaaggcaac gatgcatagc cgacctgaga gggtgatcgg ccacactgga
    39661 actgagacac ggtccagact cctacgggag gcagcagtag ggaatcttcc gcaatgggcg
    39721 aaagcctgac ggagcaacgc cgcgtgagtg atgaaggtct tcggatcgta aaactctgtt
    39781 attagggaag aacatatgtg taagtaactg tgcacatctt gacggtacct aatcagaaag
    39841 ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttatccggaa
    39901 ttattgggcg taaagcgcgc gtaggcggtt ttttaagtct gatgtgaaag cccacggctc
    39961 aaccgtggag ggtcattgga aactggaaaa cttgagtgca gaagaggaaa gtggaattcc
    40021 atgtgtagcg gtgaaatgcg cagagatatg gaggaacacc agtggcgaag gcgactttct
    40081 ggtctgtaac tgacgctgat gtgcgaaagc gtggggatca aacaggatta gataccctgg
    40141 tagtccacgc cgtaaacgat gagtgctaag tgttaggggg tttccgcccc ttagtgctgc
    40201 agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa
    40261 ttgacgggga cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac
    40321 cttaccaaat cttgacatcc tt
//
LOCUS       DANJFZ010000011        38871 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.011, whole genome shotgun sequence.
ACCESSION   DANJFZ010000011 DANJFZ010000000
VERSION     DANJFZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.011"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            131..478
                     /gene="sarR"
                     /locus_tag="PWN45_000462"
     CDS             131..478
                     /gene="sarR"
                     /locus_tag="PWN45_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDL0622251.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(667..1503)
                     /locus_tag="PWN45_000463"
     CDS             complement(667..1503)
                     /locus_tag="PWN45_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDL0622252.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1503..2117)
                     /gene="ureG"
                     /locus_tag="PWN45_000464"
     CDS             complement(1503..2117)
                     /gene="ureG"
                     /locus_tag="PWN45_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDL0622253.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2130..2819)
                     /locus_tag="PWN45_000465"
     CDS             complement(2130..2819)
                     /locus_tag="PWN45_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDL0622254.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2812..3264)
                     /gene="ureE"
                     /locus_tag="PWN45_000466"
     CDS             complement(2812..3264)
                     /gene="ureE"
                     /locus_tag="PWN45_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDL0622255.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3277..4992)
                     /gene="ureC"
                     /locus_tag="PWN45_000467"
     CDS             complement(3277..4992)
                     /gene="ureC"
                     /locus_tag="PWN45_000467"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDL0622256.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4989..5399)
                     /locus_tag="PWN45_000468"
     CDS             complement(4989..5399)
                     /locus_tag="PWN45_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDL0622257.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5413..5715)
                     /locus_tag="PWN45_000469"
     CDS             complement(5413..5715)
                     /locus_tag="PWN45_000469"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDL0622258.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5975..6880
                     /gene="yut"
                     /locus_tag="PWN45_000470"
     CDS             5975..6880
                     /gene="yut"
                     /locus_tag="PWN45_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDL0622259.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7097..8251
                     /locus_tag="PWN45_000471"
     CDS             7097..8251
                     /locus_tag="PWN45_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL0622260.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8465..9373
                     /locus_tag="PWN45_000472"
     CDS             8465..9373
                     /locus_tag="PWN45_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0622261.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9772..10713
                     /locus_tag="PWN45_000473"
     CDS             9772..10713
                     /locus_tag="PWN45_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL0622262.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10834..11388
                     /locus_tag="PWN45_000474"
     CDS             10834..11388
                     /locus_tag="PWN45_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL0622263.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            11460..12230
                     /locus_tag="PWN45_000475"
     CDS             11460..12230
                     /locus_tag="PWN45_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622264.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12402..13199)
                     /gene="fdhD"
                     /locus_tag="PWN45_000476"
     CDS             complement(12402..13199)
                     /gene="fdhD"
                     /locus_tag="PWN45_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL0622265.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13489..14271
                     /gene="modA"
                     /locus_tag="PWN45_000477"
     CDS             13489..14271
                     /gene="modA"
                     /locus_tag="PWN45_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0622266.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14285..14956
                     /gene="modB"
                     /locus_tag="PWN45_000478"
     CDS             14285..14956
                     /gene="modB"
                     /locus_tag="PWN45_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL0622267.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14957..15562
                     /locus_tag="PWN45_000479"
     CDS             14957..15562
                     /locus_tag="PWN45_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0622268.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15732..16736
                     /locus_tag="PWN45_000480"
     CDS             15732..16736
                     /locus_tag="PWN45_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL0622269.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16767..17273
                     /locus_tag="PWN45_000481"
     CDS             16767..17273
                     /locus_tag="PWN45_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDL0622270.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17275..17769)
                     /gene="moaC"
                     /locus_tag="PWN45_000482"
     CDS             complement(17275..17769)
                     /gene="moaC"
                     /locus_tag="PWN45_000482"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL0622271.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            17832..19091
                     /locus_tag="PWN45_000483"
     CDS             17832..19091
                     /locus_tag="PWN45_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL0622272.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19088..19573
                     /gene="mobB"
                     /locus_tag="PWN45_000484"
     CDS             19088..19573
                     /gene="mobB"
                     /locus_tag="PWN45_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDL0622273.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19587..20033
                     /locus_tag="PWN45_000485"
     CDS             19587..20033
                     /locus_tag="PWN45_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDL0622274.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            20039..20272
                     /gene="moaD"
                     /locus_tag="PWN45_000486"
     CDS             20039..20272
                     /gene="moaD"
                     /locus_tag="PWN45_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDL0622275.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20279..20878
                     /gene="mobA"
                     /locus_tag="PWN45_000487"
     CDS             20279..20878
                     /gene="mobA"
                     /locus_tag="PWN45_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDL0622276.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20898..21920
                     /gene="moaA"
                     /locus_tag="PWN45_000488"
     CDS             20898..21920
                     /gene="moaA"
                     /locus_tag="PWN45_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL0622277.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(22315..22485)
                     /locus_tag="PWN45_000489"
     CDS             complement(22315..22485)
                     /locus_tag="PWN45_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622278.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            22611..22961
                     /gene="sarV"
                     /locus_tag="PWN45_000490"
     CDS             22611..22961
                     /gene="sarV"
                     /locus_tag="PWN45_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDL0622279.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(23063..24274)
                     /locus_tag="PWN45_000491"
     CDS             complement(23063..24274)
                     /locus_tag="PWN45_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0622280.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(24267..24707)
                     /locus_tag="PWN45_000492"
     CDS             complement(24267..24707)
                     /locus_tag="PWN45_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDL0622281.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            24869..25633
                     /locus_tag="PWN45_000493"
     CDS             24869..25633
                     /locus_tag="PWN45_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0622282.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(25625..25876)
                     /locus_tag="PWN45_000494"
     CDS             complement(25625..25876)
                     /locus_tag="PWN45_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622283.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            26265..27530
                     /gene="femX"
                     /locus_tag="PWN45_000495"
                     /gene_synonym="fmhB"
     CDS             26265..27530
                     /gene="femX"
                     /locus_tag="PWN45_000495"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDL0622284.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            27647..30814
                     /locus_tag="PWN45_000496"
     CDS             27647..30814
                     /locus_tag="PWN45_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL0622285.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(31001..31318)
                     /gene="mspA"
                     /locus_tag="PWN45_000497"
     CDS             complement(31001..31318)
                     /gene="mspA"
                     /locus_tag="PWN45_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDL0622286.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(31563..31727)
                     /locus_tag="PWN45_000498"
     CDS             complement(31563..31727)
                     /locus_tag="PWN45_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622287.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            31860..32768
                     /locus_tag="PWN45_000499"
     CDS             31860..32768
                     /locus_tag="PWN45_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL0622288.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            32939..33070
                     /locus_tag="PWN45_000500"
     CDS             32939..33070
                     /locus_tag="PWN45_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622289.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(33187..34050)
                     /locus_tag="PWN45_000501"
     CDS             complement(33187..34050)
                     /locus_tag="PWN45_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDL0622290.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(34231..35118)
                     /locus_tag="PWN45_000502"
     CDS             complement(34231..35118)
                     /locus_tag="PWN45_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622291.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            35184..35279
                     /locus_tag="PWN45_000503"
     CDS             35184..35279
                     /locus_tag="PWN45_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622292.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            35305..37440
                     /locus_tag="PWN45_000504"
     CDS             35305..37440
                     /locus_tag="PWN45_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0622293.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            37553..>38871
                     /locus_tag="PWN45_000505"
     CDS             37553..>38871
                     /locus_tag="PWN45_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0622294.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYF"
BASE COUNT        13680 a         5705 c         7145 g        12341 t
ORIGIN      
        1 taaaaatatt gaatttttat acttatttgt ttagaatgaa ctttataaca tagttggata
       61 gagtttcgat ttaataaatt acatgtgaac cttgctacaa caagatgtgc atcagaggag
      121 tggtttaata atgagtaaaa ttaatgacat taatgattta gtcaacgcaa catttcaagt
      181 taagaagttt ttcagagata caaaaaagaa gttcaatttg aactatgaag aaatttatat
      241 tttaaatcat attttaagaa gtgagtctaa cgaaatctca tctaaagaga ttgctaagtg
      301 ctcagagttc aaaccttact atttaactaa agctttacaa aagctaaaag atttaaaatt
      361 gttatcaaag aaaagaagtt tacaagacga aagaacagtt attgtttatg ttacagatac
      421 acaaaaagca aatattcaaa aactgatttc agaattagaa gaatacatta aaaattaaat
      481 caaggttaat tgcgtttaat aacattgaac gataacaatt tattaaaacg aagttattta
      541 ttcagcattg ggacataaaa ttaacttaaa atttaaatat tgaagatgct ttaattaaag
      601 ttaaagacta gccatacctt atttcagctt attaagcttg aaacaaggta cactagtctt
      661 tttattttaa tattttctta gaaaatcaag tttacgatca taaatatttt ctgcgatata
      721 gctttggatg gttccaagta ttttctctat aatttgtgtg cgataagcaa aaattctaac
      781 tgcaaaacca tgtgtaggca attgagaaat agcaacacga caatcgaatg tattgctata
      841 agaactaatg gtttcataaa ctgaatcgat tagtttttgg ttaacatctt cgtgtatgaa
      901 gtaagcagag ccataatgag agtaatgttc catataacct atttcattga tcgattgttt
      961 attaggattc aataataaat tatcatatgt gactaactca tcttcaatat aaatttcatt
     1021 tattaaatgc atatattgat acttgaaggc ttcgccagtt tttgaataac caggagttaa
     1081 aatatcagta taaaataatg aactagaatt attgagattg aacgtattgt gttgataaaa
     1141 tttagcattt tcatatgcga tgattggatc agcgacatat tctaaatatg cgttatcttt
     1201 caaattaaaa gtttgatact gctcaacatg attggaaggt gtcttgtata tttttgttgc
     1261 accttgagat gtcaatgtca ctttagcgtt atcttcgaca ttcacattca tacggtaacg
     1321 atctccatct aaatagccac cacctacatt tactatataa aatgtaggaa tggtagattg
     1381 atttagataa actggacgta tcactttaag tgctttttca aagaaaatat ctcttgatac
     1441 tgatcgattg ccatcgaaaa acactgttaa atcaagttgc ccagtccatt gttgttcatc
     1501 cattatgata atcctttgag taaagtgtcg cgttcaatcc aatcgataac ttcatccaaa
     1561 ccttcgtcgg tttttaagtt agtaaaagta aatggacgtt taccacgaaa tactttagta
     1621 tcttcagcca tttgttctaa tgatgcacct acatagggag ctaaatcagt tttgttaatt
     1681 acaaagaaat ctgacttaat cataccttga ccacctttac gtggaatctt ttcaccttga
     1741 gcaacatcga taatatatat tgaaaagtca acaagttctg gactaaatgt tgctgctaag
     1801 ttatcgccac cagattctat gaaaattagt tctatatcgt catgacgttc taataattcg
     1861 tctattgctg caaagttcat agatgcatct tcacgaatcg cagtatgagg acatccacca
     1921 gtttcaacac caatgatacg actttcaggt agaactcctg aatttactaa tatcttttcg
     1981 tcttcttttg tatatatatc atttgtaata acgccgatac tcatttcttt tgaaagacgt
     2041 tttacaactt tttcaattaa ttgtgtttta cctgcaccta caggaccacc aataccaatt
     2101 ttaatcggat ttgccacaat tataacctcc tatgatatga aaattctaac attgacgttc
     2161 tcatgcgcca tttgatttag ttctaaacca ggcgctgtca tgccaaaatc tgcttctttt
     2221 aattcgaaaa tctgctttct tgttccttct atataaggaa tcatgtgagt aactatcttt
     2281 tgaccagcag tttgtccaag tggaatagca cgaacagcat tttgagttaa acttgaaaca
     2341 ttttgatata aatagtaatc aataatcgtt tcaatatcta cacctaaatg atggcctagc
     2401 atagtaaaac aaatagctgg atttaacttt gctttcttat cttgcatttg ttgatgatac
     2461 caagcaatcc atgggctatt ataaagttct aaagccaatt taaccatgcg agtccccatt
     2521 tgttttgcac caacacgtgt ttctttaggt aagttttgaa caaacatcag tttatctatg
     2581 tgtaatactt tttgtgtatc atcattttcc aatgcatcat aaactaaacg catggctaaa
     2641 ccatcagaat aggtaagttg ctcttgtaaa aacattttta accaagcaat aaaagtatga
     2701 tcgtcatgaa ttatatttcg ttgaatatat gtttcaagac caaatgaatg actgaaagca
     2761 cctgttggaa actgtgaatc acagaactga aataatctta agtgtgtatg atcaatcatg
     2821 agaatgccct atatgtctga aagccttatt aactttacgg tcttctcgaa catatgggat
     2881 gcctaaactt tttaataaat cttcaactaa ataatcatat tgtactagca tttcagtctc
     2941 tgtaaattgt gctggcaaat gacgatttcc taattgatgg gctatatctc ccatttcttg
     3001 caatgttctt ggttgaatca ctaaaagatc ttctgaatta acatccacaa taatcatatt
     3061 atggtcatct gcgtataaaa tatctccata ttgtaagtca ataggttgtt ttaaacgaat
     3121 gcctatttca gtgccatggt ctgtaacgac tctttgaata cgtttaacaa gatctgaatt
     3181 ttcaaggtat actttttcga cgtgcttttg tttttctgaa tttgacaaat tggcaatatt
     3241 gccttggatt tcttcaacaa tcattctatg ttcctcctag aataagaagt atctttgagt
     3301 taatggtaac tcagttgctg cattacttgt aattttttct ccatctacat atacttcata
     3361 tgtttgtgga tcaacgtcta atttaggtgt tgcactgtta tttttcatat ctgctttaga
     3421 taattgtcta atatttttaa ctggacgcac catgcgtttt aaatttaatg cacgattgat
     3481 accattttca taagcagttt tagacacgaa tgtcattgac gtacttgtaa ggtttccgcc
     3541 gtattgacca tacattttac ggtacttcat cggttcagat gtaggtatag aaccatttgc
     3601 atcgccattt acggcagagt taattaatcc gccctttaca actaattcag gtttaacccc
     3661 aaagaaaatt gggtcccata agacaatgtc agctagtttg cccggctcga tagatcctac
     3721 atattcagaa ataccatgtg taattgctgg gttaattgta tatttagcga tataacgttt
     3781 gatgcgatta ttatcattat gttcaaaatc accatctaaa ggaccacgtt gttctttcat
     3841 gcgatgtgct acttgccatg ttcgtgtaat tacttcacct acacggccca ttgcttgtga
     3901 atcggaacta atcatactga atacacccat atcttgcaga acatcttctg ctgcaatcgt
     3961 ttctttacga atacgtgaat ctgcgaatgc gatatcttca ggaatagccg catttaaatg
     4021 gtgagtaatc attaccatat ctaaatgttc atctacagta ttatgtgtat aaggcaaagt
     4081 tggatttgta gatgaaggta aaatatttga aaatgcagcg gatttaatta aatcaggcgc
     4141 atgaccgcca ccagcacctt cagtatggta catatgaagt acacggtctt taacagcagc
     4201 cattgtgtct tccataaatc ctgcttcatt taaagtatct gcatgtaatg caatttgaac
     4261 atcaaattca tcagcaacat ctaatgcatg actcaaagca gatggtgttg caccccagtc
     4321 ttcatgtact tttaatccaa ttgctccggc attgatttgt tcaatgagtg cagttggatt
     4381 tgttgcttgt cctttacctg taaaaccgac attaatcggt aaaccttcgg cagcttctaa
     4441 cattctatga atatgccatg gacctggagt tacagttgtt gctttagaac cttctgaagc
     4501 accagtacca ccaccaatat gagtcgtaat accactttct aatgcgacct ctgcttgttc
     4561 aggattaata aaatgaacat gagtatcaat accaccagca gtgacgattt taccttcagc
     4621 ggcaatgata tctgttgttg aacctataat aatgtcgaca ttatccatta tatctgggtt
     4681 gccggcatta cctatggcga aaatataacc atttttaatg cctatatcag ctttaaccac
     4741 tttatcgtaa tcgataataa cggcattaga aatgacaagg tctgcaacgt tcacgtcatc
     4801 acgtgttaca cgaggatttt gcgccatacc gtctctaata gatttaccac caccaaaagt
     4861 agcttcttca ccataaaccg catagtcttt ttctatttga gcaaatagat tcgtatcacc
     4921 taaacgaatg gaatctccaa cagttggacc gtataagctc gtatattgat tttgcgtcat
     4981 tttaaagctc atgatctttt tcctcctttt ttattcacgt tttcagcacc gttatctccg
     5041 aatacacctg catattcatc attttcatca gttgggcgat agacacgtga ctcatcgata
     5101 ggaccattga ccataccacg aaaaccaaaa attttacgtt tgccagcata ttcaactaat
     5161 tgaacttctt ttttatcccc aggttcaaat cgaacagctg ctccagctgg aatatctaaa
     5221 tgttttccat atgccatttc acgttcgaaa tctaatgctg catttgcttc ataaaaatga
     5281 aaatgtgagc ccacttgaat tggtcgatct cctgtatttt caacttcgat aactgtttca
     5341 ggatgatggt tattaatttc aacctctgta ctttttgtaa taatttctcc tggtatcatt
     5401 tgactgcctc ctttaaacaa tagggtgatg tactgtgatt aacttagtac catcggggaa
     5461 cgtagcctcg atttcgatat ctgtaatcat gtgttcgaca ccatccatga catcttcttt
     5521 gtttagaatt tgtctaccat aactcattaa ctctgcaacg gtcttaccat cgcgtgcacc
     5581 ttctaataat tcatcgctga ttaaagctaa tgcctcagga tgatttagtt tcaaaccacg
     5641 tgctttacga cgacgtgcaa cttccgccgc cactacaatc attaatttgt cttgctctcg
     5701 ttgtgtaaaa tgcaaattaa aacccccaat ttcatattag atacaattta caaaatttat
     5761 attaatccta attgttgtga taaacaagta atatacaaag ttcaatgtgt aattagaaaa
     5821 ttatattttt agcatatccg atattgaagc aaacaatcta atcgaaaaca aatagtggaa
     5881 tatatttatg taaaaaccaa aatagttttt aatataactt ttcatagaat agtagtatat
     5941 taatgagtaa tgattcaaag gaaaggtgaa agatttgaag ataatagatg tgcttttgaa
     6001 aaatatatct caggttgtgt taattagtaa taaatggaca ggattattta tcttaatagg
     6061 attatttgta gccgattgga caattggatt agcggctatt gtaggtagca tcatcgccta
     6121 tacttttgcg cgttttataa attatagtga ggcagagatt aatgatgggt tagctggatt
     6181 taatccagtg ctaactgcca ttgcgttaac aatcttttta gataagtcag gattagatat
     6241 tgttataaca atgatagcaa ctttattaac gttaccagtt gctgctgcag tgagagaagt
     6301 tttaagacca tataaagttc cgatgctgac gatgcctttt gtcattgtga cttggtttac
     6361 aattttactt tcaggacagg ttaaatttgt agatacatcg ttaaagttaa tgcctcaaaa
     6421 cattgaaacg gttaatttta gcaacaatga tagaatacat ttcattcagt cattatttga
     6481 aggattcagt caagtattta tcgaagcgag tgtaattggt ggcgtatgta ttttaatcgg
     6541 catattgata gcatcaagaa aagcaacact cttagctgtt atagctagtt tgttaagctt
     6601 tatcattgta gctctattag gtggtaatta tgatgatatt aatcagggat tattcggtta
     6661 taactttgta ttaatggcaa tcgcactagg atatacattt aaaacagcga ttaaccctta
     6721 tatttcgact tttttaggtg tgttattaac agtagtggtg caactaggta caacaacatt
     6781 gcttgaaccg tttggcttac ctgcattaac attgccattt attatcgtga catggatttt
     6841 attatttgct ggtattaaac atgacaaagt agatgcttga tagttaaatc aaacctaata
     6901 ttgtttgaat atcaccttaa actatacagc gaattgtata gtttaaggtg tatttttatg
     6961 gataaaatta agtgcatact taatcgaatt atcagttttc ttttaattgt tcacagcacg
     7021 ttccttttta tttttaaagt cgagtgttaa actaggaata aataatttat attgtgtgtt
     7081 gtgtggggtg actaatatga atgatatgga taattccttt ttaataacaa cggaaattca
     7141 aagaaaatgg attgaaaaat tcaaagtaat tagagataca tttaaggcta aagctgaata
     7201 taatgatcaa catagccaat ttccatataa aaatattgaa tggttaatta aagaaggtta
     7261 tggaaaatta acgttaccaa aagcatatgg tggtgaaggt gcgaccatag aagacatggt
     7321 tattttgcaa tcatttttag gcgaacttga tggtgccaca gcattatcta ttggttggca
     7381 tgtgagtgtc gtaggacaaa tttatgaaca gaaattatgg tctcaagata tgttggagca
     7441 atttgctgtt gaaattaata atggtgcatt agttaataga gcagttagtg aagctgaaat
     7501 gggtagtcca acaagagggg gaagaccaag tacacatgct gttaaagctg atgatgggta
     7561 tattttaaat ggtgtgaaga catatacatc aatgagtaaa gcactaacac atattattgt
     7621 tgctgcttat atagaagaat tagaaagtgt tggttttttc ttagtagaca ggaatttgcc
     7681 tggtgttgaa attgccgata attgggacgt attaggtatg cgagcgacag aaagtcatga
     7741 tttaatatta aatgatgtaa aagtaccatt aaaacattta gtggaaacag agaaaagtaa
     7801 agcgccaaat ggttggatat tgcatatacc aagctgttat ttaggtatag cacaagctgc
     7861 tagaaattat gctgtagact ttgcaatcca acatagccct aatagtattg aaggaacgat
     7921 tgcaacattg ccaactgtgc aacaaaattt agggaaaatg gaaacgctat tgttatgtgc
     7981 gagacaattt ttatggagta cagcaaaagg gtatcaacaa tataaagatg acagtcagat
     8041 aagaaatcca acaagtgcga gtaaagtgat ggtaatgaat caagggcttg aagtgattga
     8101 tttagcgatg agaatagttg gtgctaaaag cttagaaatg aatcgaccat tacaacgtta
     8161 ctatcgtgac atgcgtgcag gattacacaa tccacctatg gaagatgcag catatacaaa
     8221 tattgctaag agtattacag atacatttta aattttaaaa ggtaaatcga tcgttgattg
     8281 gcataaagag atgaaatttg acgttcggat ttaccctttt tttgtgggga tttataggga
     8341 tgttaagata ttttgttaaa ttttttgcga aaataaaaaa taaataacac tagctattgt
     8401 aaatgtatat gcaatcttgt ataatttttg aatgattgat aatcattttc aataggagga
     8461 aattatgaaa aaactattat taccattaat aattatgtta ttagtgttag ctgcgtgtgg
     8521 gaaccaaggt gaaaaaaata acaaagctga aactaaatct tataaaatgg acgatggcaa
     8581 aacggtagat attccgaaag accctaaacg cattgcagta gttgcgccaa catatgctgg
     8641 tggacttaaa aaattaggtg caaacattgt agctgtaaat caacaagtcg atcaaagcaa
     8701 agtattaaaa gataaattta aaggtgttac aaaaattggt gatggcgatg tagaaaaagt
     8761 tgctaaagaa aagccagatt taattattgt atactctact gacaaagata ttaaaaaata
     8821 tcaaaaagta gcaccaacag tagttgttga ctataataag cataaatatt tagaacaaca
     8881 agaaatgtta gggaaaattg ttggtaaaga agataaagta aaagcttgga agaaagattg
     8941 ggaagaaaca actgctaaag acggtaaaga aattaaaaaa gcaattggac aagatgcaac
     9001 agtgtcattg tttgatgaat ttgataaaaa attatacact tacggcgata actggggtcg
     9061 tggtggagaa gtattatatc aagcatttgg tttgaaaatg caaccagaac aacaaaagtt
     9121 aactgcaaaa gcaggttggg ctgaagtgaa acaagaagaa attgaaaaat atgctggtga
     9181 ttacattgtg agtacaagtg aaggtaaacc tacaccagga tacgaatcaa caaacatgtg
     9241 gaagaatttg aaagctacta aagaaggaca tattgttaaa gttgatgctg gtacatactg
     9301 gtacaacgat ccttatacat tagatttcat gcgtaaagat ttaaaagaaa aattaattaa
     9361 agctgcaaaa taattcagct atataagtta gtgaaatgag agtctgaaac atatcaatct
     9421 tttgatattg tattaggctc ttatttttat agctagaaag ttagatattt gtattttttt
     9481 aaataataag tgccgttgtt atcgttcaat ttaattaatg atagattagt attattatag
     9541 ctaaagtagt atacctgaga aaatagctca atgtatctct ttattaataa gttatatcat
     9601 aattatttta gtgcatactt tatggaaggg atatcaggga atggctttca attaaagaag
     9661 aggtttaaaa ggattacaac agaatgttat gattttgtag aaagatatat aacaacgttt
     9721 tataaaaaca taatattgtt aatggaaaat gaaatgtaag ggggatttcg agtgactaag
     9781 aaagtttatt ttaaccacga tggtggtgta gatgatttag tatctctatt tttattatta
     9841 caaatggaaa acgttcaatt gataggggtc agtacaattg gtgctgattg ttatttagag
     9901 ccatctttga gcgcatcagt aaaaattatt aatcgttttt caaatgaaga tattcaagtt
     9961 gcgccatcat atgaacgagg aaaaaatcca tttcctaaag aatggcgtat gcatgccttt
    10021 tttatggacg cattgccaat tttaaatgag ccagtcaaac atgttgcttc aaatgtgagc
    10081 gacaaagaag cctttgaaga cattattcaa actttaaaga gacaatcaga aaaagtaaca
    10141 ttattattta caggcccgct tacagattta gcaaaagcac tacaaaaaga ttcatctatc
    10201 gttcagtata tagaaaaatt agtttggatg ggtggcacct ttttaccaaa aggaaatgtt
    10261 gaagaacctg agcatgatgg ttctgcagaa tggaatgcat attgggatcc agaagcggtt
    10321 aaaattgttt ttgatagcga tatagagatt gatatggttg ctttagaaag tacgaatcaa
    10381 gtaccgctaa cgttagatgt tagacaaaga tgggcgaatg aacgccaata tacgggcatt
    10441 gattttttag gagtaagtta tgcagcggta ccaccattaa cacactttat aacaaattct
    10501 acttactttt tatgggatgt tttaacgact gcttatattg gtaacaagga cttggttcat
    10561 tcaattgaga aaaaagtcga tgtaataagt tatggaccaa gtcaaggtaa gacatttgag
    10621 tgtaaagatg ggcgcaaaat taatgtcata aatcatgtag ataacaacgc attttttgat
    10681 tatataactg cacttgctaa aaaagtaaat taacagctgt gtagaataat taaggtttta
    10741 atttatatag aacaacttat tgtaaacttt tcatttctta aagtttacaa tggtgctata
    10801 ataatggtca tgaaatacga aaggaagtaa aaaatgacaa caaaacagtt agtatataca
    10861 gctttaatga cagcgattat cgctatttta ggattggtac cggtaattcc actaccattt
    10921 tcttcagtac caattgtact tcaaaacatt ggtattttct tagcaggtgt gattttagga
    10981 cgtaaatatg gcacattaag tgttatcgtc tttttattat tagtagttgc tggcttgcca
    11041 ttgttatcag gtggtcgcgg tggcatcggt gtattcgcag gtccttcagc agggttttta
    11101 ctattatatc cagttgtagc attcatgatt ggggcgattc gagatagatt catcaatgaa
    11161 attaatttct ggattttatt cgttggtatt ttagtttttg gtgttatagc attagatgtt
    11221 attggtacat tgattatggg catgattatt aacataccat ttacgaaagc tatttcaatt
    11281 tcattagctt atttgcctgg tgatatatta aaagcaattg tagcaagttt gattggtaca
    11341 gctttactta atcactcgca gtttcgtcaa attatgggaa taaaataatc atatttaaga
    11401 tagtaaagta attgaataag ttgctttgaa atttataaaa gtgaaaggag taggtgtcaa
    11461 tggctagtat aagtatgtca gatatatatt gtaacggcac tatatttgaa aatgacgacg
    11521 agcagttgat ttatttaacg ccttcttttc cacaacgata cacaagtaac acatggatat
    11581 ataaaaagac gcctacccaa gagcgatggc tgaaagactt agaacgtcaa catcaattac
    11641 atacaaatca aggttcaaat cattatgcgt ttagtttccc ggaaaatgaa caacttgata
    11701 atcattggat ggctatgttt aaagatatga attttgaact aggtattatg gaattgtatg
    11761 ccatagaaag tgatgcgctt gccaatttgc cgcgtaactc tgacgttgaa attgccatcg
    11821 ttgacgagtc gcatatagat gcctatttaa aagttgcata tcagtttagt ttgccatttg
    11881 gaaaagacta tgcagatgca catgaagaaa tggtaaggga acattatcaa aaagatgtga
    11941 ttaaacgctt agtagcttat ttaaataatg aacctattgg cgttgtagat gtcattgaaa
    12001 gtgaaaatta cattgaatta gatggatttg gtgtattaga acaatttcgg caccaaggaa
    12061 ttggatctac aattcaatcg ttgataggtg aatacgccat atcaaaaaat cacaaaccaa
    12121 tcatattagt tgcagatggt gaagatacag caaaagatat gtatgcaaag caaggttatg
    12181 tctatcaatc gttttgttat caaatattaa aagaagatat tggaaattaa cgattgatga
    12241 taggtttgta ttagaataaa aaagcagtaa gctggtttcg agtagaaatc aacttactgc
    12301 tttttaaatt gttttgagct acttatactt ataaaaatag tgcgtttaaa ttgttgattc
    12361 atgtagaata tcgttcatta tgacacacta taatgaatat gttattgttc agaatcaatg
    12421 atacgttctg gatgactgta tatattaaag ccaccatttc gaataaatcc aactgccgta
    12481 atatttaggt cattagctaa ggttacagca agcgttgtcg gagctgattt agataaaatg
    12541 acgccaacac caatttttgc ggctttaatt aaaatttctg atgaaatacg tccactaaaa
    12601 attaatactt tatctcggac agtaatatgt cgctgaatac aaaatccata taatttatct
    12661 agagcgttat gtctaccaat gtcttgtcga tgtacaaaaa atgtcaaacc atcgcttata
    12721 gcagcattat gtaagccacc tgtttcttgg taaatatgac ttgcactttg taatcgagtc
    12781 atcatgttaa taatttgcat tggagttaaa gtgattttag acatagatgt tttagcgata
    12841 gcagcatcat tttgaaaata aaactcacga ctctttccgc aacaagatgc aatcattcgt
    12901 tttgtggaat attgaaagcg atcgcctaaa tctttattaa gttcaacatg ggcaaaacct
    12961 ttactatcat caatcagtac agattttaat tcatctcgct ttaaaatggc accttccgaa
    13021 gccagaaatc caatgactaa ctcctcaagg tttgttggac tgcatataac agtcgcaaat
    13081 tcttcaccat tcaccataat tgtaagtgga aattctgtca catattgatc tgttgtattg
    13141 aataattttc catcttcata tctaacaatt ggttgaccta aagatacatc tttgttcatt
    13201 atctaacccc tttaattagc ttaaacttta ttttaaagca atttgcttaa aattttaaca
    13261 tatttgctta agtttgaaat ttgattgata aaaattaata gcgagcaatc tgtttgattt
    13321 aaattgaatt cgagaatata catactaggg catcaattaa taaatatcaa tcttatgcaa
    13381 atttgacaat tgtttgaatc aatatataaa caggcaacgg ttcttttcaa atataatagt
    13441 aagtgtataa tgaaaatgta aatattatta aaaatggggg ttcactcaat gaaattgaaa
    13501 cgtttatttg ctgttgtgat tgcaatgctt ttagtattag ctggttgctc taattctaac
    13561 gataataatg aaagtaaaaa agatgacgca gacaatggta agaaacaaga gattcaagtt
    13621 gcagcggcag caagtttaac agatgtaacc aagaaattag cttcagaatt taaaaaagag
    13681 cataaaaatg ctgatattaa atttaactat ggtggatcag gggcattaag aaaacaaatt
    13741 gaatcaggcg cacctgttga cgtatttatg tctgcaaata ctaaagatgt agatgcatta
    13801 aaagacaaga ataaagcgca tgatacatat aaatatgcga aaaatagtct agtattaatt
    13861 ggtgataaag attcaaatta cacttcagta aaagacttaa aagacaatga taaattagca
    13921 ttaggtgaag tgaaaactgt accagcagga aaatatgcga aacagtattt agataacaat
    13981 aacttattta aagaagtcga aagtaaaatc gtttatgcta aagatgtaaa acaagtatta
    14041 aattatgttg aaaagggtaa tgcgaaacaa ggttttgtgt ataaaactga cttatataaa
    14101 caaaataaaa aaattgatac tgtaaaagta attaaagaag tagaacttaa gaagccaatc
    14161 acatacgaag ctggtgctac atcagatagt aaattagcaa aagagtggat ggaattctta
    14221 aaatcagata aagctaaaga aatactaaaa gaataccact ttgcagcata aggagttgta
    14281 atccatgcct gacttaacac ctttttggat atcaatacga gttgctgtaa tcagtacgat
    14341 tattgtaacg gttttaggta tttttatatc taaatggttg tatcgtcgta agggttcgtg
    14401 ggttaaatta ttggaaagtt tattgatatt acctattgtt ttgccgccaa cggtattagg
    14461 ttttattcta ttaatcatct tctcgccaag aggaccaatc ggtcaattct ttgcgaatgt
    14521 actacattta cctgtagtgt tcactttgac aggtgctgtg atagcatctg tcattgttag
    14581 ttttccacta atgtatcaac atactgtgca aggcttcaga ggtatagaca cgaaaatgat
    14641 taatacagct agaacgatgg gagcaagtga aacgaaaatt ttcctcaaat taattttacc
    14701 attagctaaa cgctctattt tagcaggtat aatgatgagt tttgctcgtg cattaggtga
    14761 gtttggtgct acattaatgg ttgcaggata tattccaaat aaaacgaata cactaccttt
    14821 agaaatatac ttcttagtgg aacaaggtag agaaaatgaa gcgtggttat gggtattagt
    14881 gctagtcgca ttctctattg tggttatatc tacaattaat ttattgaata aagataaata
    14941 taaggaggtc gactagatgc ttaaaatcaa tgtgaaatat caattaaaga acactttaat
    15001 tcgcatcaat atagatgata ctgaaccaaa aatttatgca gttcgtggtc catctggcat
    15061 tggtaaaact actgttttaa atatgattgc cggattacgt aaagcagatg aagctattat
    15121 cgaagtgaat gggcaattac ttactgatac ggcaaaaaac gtgaatgtta aaattcaaca
    15181 acgacgtatt ggatatctgt ttcaagacta ccaattgttt cctaatatga cggtctataa
    15241 aaatattact tttatggctg aaccatctga acacatcgat caattaattc aaactttaaa
    15301 cattgatcat ttgatgaaac aatatcctat gacattgtca ggtggagagg cacaacgtgt
    15361 agcacttgca cgtgcactta gcacgaaacc agatttaatt ttattagatg aacctttttc
    15421 tagtttggat gatactacaa aagatgagag tattacatta gttaaacgta ttttcaacga
    15481 atggcaaata ccaatcatat ttgtgacaca ttcaaactat gaagcagaac aaatggctca
    15541 tgaaattatt acaattgggt aatcatttat ttgccattaa agagtttaga acgtatttaa
    15601 aattgtagaa gtgaatgctt ctatcagcat tttaatgatg ttttaaactc ttttttaggg
    15661 gcagtttttt tgagagacat tgacgcgcgt catataatga aagtaatgat aaaaagaaag
    15721 gataacttaa tgtgagtcaa gaacgttatt caaggcaaat tttatttaaa caaataggtg
    15781 aaataggtca aagcaaaata aatcaaaaat gtgcgttgat tattggtatg ggcgcattag
    15841 gtacacatgt ggccgaagga cttgttagag caggcattgc caaactaatc attgttgata
    15901 gagattatat tgaatttagt aatttacaaa gacaaacatt gtttactgaa gaagatgctt
    15961 tgaaaatgat gcctaaggtg gttgcagcta aaaagcattt gctagcgtta cgtagtgatg
    16021 ttgatattga tgattatatt gcccatgtgg attattattt tttggaaaca catggacagg
    16081 acgttgacgt tattattgat gcaaccgata actttgaaac acgacaactg attaatgatt
    16141 ttgcatataa atatcgtata ccttggattt atggtggtgt tgtacagagt acatatacag
    16201 aagctgcatt tatacctggt aaaacacctt gctttaactg tttggtacca caattgccag
    16261 cattaaattt aacatgtgat acagtagggg tcattcaacc tgccgtgacg atggcaacaa
    16321 gtttacaatt aagagatgcg atgaaagtat taacggaaca accaattgac acaaaaataa
    16381 cttatggcga tatttgggaa ggtagtcatt attcatttgg tttcagtaaa atgcaacgtt
    16441 cagactgtac aacttgtgga gatgtaccaa gttatccgta tttaaacaag aatgaacaac
    16501 gttatgcaac attgtgtggt agagacactg tacagtatga aaatgcatca attacacacg
    16561 acattcttgt tcaattttta aaacaacatc agttaaatta tcgcagtaat tcgtatatgg
    16621 ttatgtttga atttaaagga caccgcattg ttgcttttaa aggtggaagg tttttaatac
    16681 atggcatgac acgcacatca gatgccacac atctaatgaa tttattgttt ggataaaaaa
    16741 agataagaca aaaggagtgt aatattatgg gcgaacatca aaacgttaaa ttgaatcgta
    16801 cagttaaagc agccgtacta acggtatcag atactagaga ctttgataca gataaaggtg
    16861 gtcaatgcgt gcgccaacta ttacaagcag atgacgttga agtgagtgac gcacattata
    16921 caattgtgaa agatgaaaaa gtagccatca cgacgcaggt gaagaagtgg ttagaagaag
    16981 atattgatgt catcattacg actggtggaa caggtattgc acaacgtgat gtgacgattg
    17041 aagcagtaaa accactttta actaaagaga tagatggctt tggggaattg tttagatatt
    17101 tgagttatgt tgaagatgtt ggcacgcgtg cattattgtc tcgtgctgta gcaggtacag
    17161 ttaataataa attgatattt tcgattccag gatcaacagg cgcagttaaa ttagcattag
    17221 aaaagctcat taaaccagaa ttaaatcatc tgattcatga gcttacaaaa taatttattg
    17281 atttgattgg cgttgaaaat ctccagattt accgccagac ttgctttcaa ggtaggtttc
    17341 gccaataatc atacctttat caactgcttt cgtcatgtcg taaatggtta aagccgttgc
    17401 tgatgcagcg gttaaagctt ccatttcaac accggttttg ccagttgtag agacagttgt
    17461 ttgaatgttt aaagtataaa ggggtgcatt tgtttcatcc cagctgaagt gaacatctat
    17521 gccagtcaat ggtaatggat ggcacatcgg aataagtgtt gatgtatttt tggcagccat
    17581 aataccagcg atttgagcag tgttcaatac attgcctttg gtattggtgt tattagaaat
    17641 ctgcttaaaa atagtttcat ttacagtaat gcttgaatga gcgacagcag ttctttttgt
    17701 aatttgtttg tctgatacat cgaccatttt ggcgtggcct tgttgattaa tatgagtaaa
    17761 ctcagtcatt ttacccctcc tagtgcatct agtatatcat gaaaaaataa aagttttgga
    17821 gatgattttt aatggtagta gaaaaaagaa acccaatccc agttaaagaa gcaattcaac
    17881 gtatcgttaa tcagcagagt tcaatgccgg caattacggt agcacttgaa aaaagtctaa
    17941 atcatatctt agcagaagat attgtagcta cttatgatat accaaggttt gataaatcac
    18001 cttatgatgg ttttgcaatt cgcagtgttg attcacaagg ggcaagtggt cagaatcgca
    18061 ttgagtttaa agtgattgat catattggtg caggttcagt ttctgataaa ttagttgggg
    18121 atcacgaagc ggtgcgtatt atgactggag cacaaatacc taatggcgca gatgctgttg
    18181 ttatgtttga acaaacgatt gaactagaag atacatttac aattcgtaaa ccattttcaa
    18241 aaaatgaaaa tatatcttta aaaggtgaag aaacaaagac aggcgatgtt gttctaaaaa
    18301 aaggacaagt aattaatcca ggggctatcg cggtccttgc aacatatggc tatgcagagg
    18361 ttaaagttat taagcaaccg agtgtcgctg ttattgcaac aggaagcgaa ttattagatg
    18421 ttaatgatgt attagaagat ggaaaaattc gtaactctaa tggcccaatg attcgtgcct
    18481 tagcagaaaa attaggtctt gaagttggta tttacaaaac acaaaaagat gatttagata
    18541 gtggcatcca agtcgttaaa gaagctatgg aaaaacatga tatcgttatt acaacgggcg
    18601 gagtttctgt tggagatttt gactatttac ctgagattta taaggctgta aaggcggaag
    18661 tgttatttaa taaagtagca atgcgtcctg gtagcgtaac aacggttgca tttgtagatg
    18721 gaaagtattt gtttggatta tctggaaatc catcagcttg ttttacagga tttgaactat
    18781 ttgtgaaacc agctgttaaa catatgtgtg gcgcactaga agtcttcccg caaataatta
    18841 aagcaacatt aatggaagat tttaccaagg caaacccatt cacacgattt atacgtgcta
    18901 aagcaacgtt aacaagtgct ggagctactg tagtaccttc aggattcaat aaatcaggtg
    18961 cggttgtagc gattgcacat gctaactgta tggtcatgtt accaggaggg tcacgtggtt
    19021 ttaaagcggg gcatacagta gatattatat tgactgaatc tgacgctgct gaagaggaac
    19081 ttcttttatg attttacaaa ttgtaggtta caaaaagtct ggtaagacaa cattgatgag
    19141 gcatattgtc tctttcttaa agtcacatgg ttatacagtt gctactatta aacatcatgg
    19201 gcatggtaag gaagatattc aattacagga ttcagacgtc gatcacatga agcattttga
    19261 agcgggggca gatcaaagta ttgtacaagg ttttcaatat cagcaaactg taacacgtgt
    19321 agataatcaa aatcttactc aaattattga aaaatctgtt acaattgaca ccaatatcgt
    19381 attagttgaa ggctttaaaa atgctgattt tgaaaaagtc gtagtctatc gaaatgaaga
    19441 agagttgcaa gtattacaac aattgtcgaa tgtttgttat agcattaatg taagggagca
    19501 tgaagatttt acagcatttg agcaatggtt attaaataaa attaaaaatg attgtgatac
    19561 acaattaaca tagaggattg aaatgaatga aacaatttga aatcgtgaca gaaccgatac
    19621 aaacagaaca atatcgtgaa ttcactataa atgaatatca aggtgcagta gttgttttta
    19681 ccggtcatgt tcgcgaatgg actaaaggcg tcaaaacgga atatttagaa tatgaagcgt
    19741 atattccaat ggctgaaaag aaattggcac aaattggaga tgaaataaat gaaaaatggc
    19801 ctggaacgat aacgagtatt gttcatagaa tagggccatt acaaatttca gatatcgctg
    19861 tattaattgc ggtttcttca ccgcatcgta aagatgccta tcgagcaaat gaatatgcaa
    19921 ttgagcgtat aaaagaaatt gttccgattt ggaaaaaaga aatttgggaa gatggttcaa
    19981 aatggcaagg gcatcaaaaa gggaattatg aagaagcgaa gagggaggaa taagagagat
    20041 gaaggtactt tacttcgcag aaattaaaga tatattacaa aaagcacagg aagatattgt
    20101 gcttgaacaa gcattgactg tacaacaatt tgaagattta ttgtttgaac gttatccgca
    20161 aatcaataat aaaaagtttc aagttgctgt aaatgaggaa tttgtacaaa aatcggattt
    20221 cattcaacct aatgatactg ttgcattaat tccaccggtt agtggaggtt aagggagcat
    20281 gaaagcaata attcttgcag gtggtcattc agtgcgattt ggtaagccca aagcttttgc
    20341 ggaagtgaac ggtgagacct tttatagtag agtaattaag acattagaat caacaaatat
    20401 gttcaatgaa attattatta gtacaaatgc gcaattggca acgcaattta aatatccaaa
    20461 tgttgttata gatgatgaga atcataatga taaaggtcca ttagcaggaa tttatacaat
    20521 catgaagcaa catcctgaag aagaattgtt ttttgtcgtt tctgttgata caccaatgat
    20581 tactggtaaa gctgtaagca cgttgtatca gtttttagtt tctcatctta ttgaaaatca
    20641 tttagatgtc gcagctttta aagaagatgg acgttttatt ccaacaattg cattttatag
    20701 tccgaatgca ttaggcgcta taactaaagc actacattct gataattaca gttttaaaaa
    20761 tgtatatcat gaattatcaa cggattattt ggatgtaagg gatgtagatg cgccctcata
    20821 ttggtacaaa aatataaatt atcagcatga tttggacgct ttaattcaaa aattgtaagc
    20881 tgttaggagg tccacaaatg gtagaacaaa taaaagataa actaggacgt cccatccgtg
    20941 acttacggtt atctgtgaca gatcggtgta actttaggtg tgattattgc atgcctaaag
    21001 aggtatttgg agatgatttc gtatttttac ctaaaaatga acttttaacg tttgatgaaa
    21061 tggctagaat cgctaaggta tatgcagaat taggtgtaaa aaaaatacgc attacaggtg
    21121 gagaaccatt gatgcgacgg gatttagatg tacttatagc taaattaaat caaatcgatg
    21181 gtattgaaga tattggtttg actacaaatg gtttgttatt aaaaaagcat ggacaaaagt
    21241 tatatgatgc tgggctgcgc agaattaatg tcagtttgga tgctattgat gatacgctat
    21301 ttcaatcaat caataatcgt aatattaaag cgactacgat tttagaacaa attgattacg
    21361 cgacgtctat tggtttgaat gtaaaagtaa atgttgttat acaaaaaggt attaacgatg
    21421 atcaaatcat accaatgctt gaatatttta aagataaaca tatagagatt cgatttatag
    21481 aatttatgga tgttggtaat gataatggat gggatttcag taaagttgta actaaagatg
    21541 aaatgcttac aatgatagag cagcactttg aaatcgatcc tgtagaacca aaatattttg
    21601 gggaagtagc aaaatattat cgccataagg ataatggtgt tcaatttggt ttgattacaa
    21661 gtgtttcaca atcattttgt tctacatgta cacgcgcaag gctgtcatca gatgggaagt
    21721 tttacggatg tttatttgca actgtcgatg gatttaacgt taaagcgttt attcgttctg
    21781 gcgtgaccga cgaagaatta aaagaacaat ttaaagcttt atggcaaata agagatgatc
    21841 gatattcaga tgagagaact gctcaaacag ttgccaatcg tcaacgtaaa aagataaaca
    21901 tgaattatat tggtggttaa tgtgtaggga ccactacata ttaaatcatt agagatgttt
    21961 taatatttct gtcttactcc ctaaaataca atattattta ttaaagtaaa aacggtcata
    22021 tctatgccag atttaataga aatgatcgtt tttaaagttt ttacaagttg gcggggcccc
    22081 aacacagaag ctgacagaaa gtcagcttac aataatgtgc aagttggcgg ggccccaaca
    22141 tagagaattt caaaaagaaa ttctacagac aatgcaagtt ggggaacggg gccccaacac
    22201 agaaggtgac gaaaagtcag catacaataa tgtgcaagtt ggcggggccc caacatagag
    22261 aatttcaaaa gaaattctac agacaatgca agttggggat caacgaaata aattttatga
    22321 gaatatcatt tctatcccac tcttaagaat cactacataa taaatcttta gtggttcttt
    22381 aacattgatg tcacactcca tgccattgag ttgtaatata tcttttttag gtataaatgt
    22441 tgtcgaataa acaacaagtt gtccaaaaga tataaatcta aacaagatat agccagcaat
    22501 ttaatatttg taatagataa aatgctaagt ttgatatata ataaatttaa gtaattgtat
    22561 aataatatga attacaaaca tctaagaaga aacataggag gcatcatatt atgagtaata
    22621 aagttcaacg ttttatagaa gcagaaaggg agttaagtca gttaaagcac tggttaaaaa
    22681 caacacataa gatttcaatt gaagaatttg tagtcctttt taaagtgtat gaagctgaaa
    22741 agattagcgg taaagaattg agggatacat tacattttga aatgctatgg gatacaagta
    22801 aaatcgatgt gattatccgt aaaatctata aaaaagagct tatttctaaa ttgcgttctg
    22861 aaacggatga aagacaagta ttctatttct atagtacttc tcaaaagaaa ttgttagata
    22921 aaattactaa agaaatagaa gtgttaagcg ttacaaacta aaaacttaaa aagcatgcca
    22981 atcagtacat cataattgcg tcttggggac agacaaatga tgaatagaga ttggcatgct
    23041 ttttattttt gaatataaat atttagttca tggcatttct agttacatga cgtccatgaa
    23101 ttaagaagta aacaagcata gtaatgattg ctaaagcggc cataaagccg aagatttcac
    23161 tatatgaaaa catatgagta aataacccaa ggaatgatgg accgaagccg acacctgcat
    23221 ctagaccaac gtaaaaagta gatgtcgcga taccatattt aatcgggggt gagactttta
    23281 tcgcaataga ttgcattgca gatgataaat ttccataccc taaacctagg caagcaccag
    23341 caagtaatat taaccagctt tgatagcttg aaattaagca tacaaatgaa aggaaaagca
    23401 tgataaatgc tgggtagaca ataatatttt catttttatc atccatcaat ctaccagcaa
    23461 taggtctagt aattaacgat gctatagcat agcaaataaa gaaatagctt gctgcagtga
    23521 ctaggtgtcg ctctaaagca aatgcttgta aataagttag gatggacgca taggtaacgc
    23581 caattaaaag cataattaca gcaacaggaa tggcctcttt tgcaataaat tgatgaatac
    23641 taaatcttgg tttatcaatg acattagttt cagttttgtt atttgttact tcgaaatcaa
    23701 cttttataaa taatgagata atgagtccga gtatgcctaa tatgacacaa ataataaaca
    23761 gtaagtcaat tgcgtatttt gtaataagta acatgcctag aaatgggcca atcgctgtac
    23821 ctaatactaa acttaaggaa aataaactga tgccttcact ttttctatta acaggggtaa
    23881 cgtatgccgc aatagtacct gttgcagttg tcacaactgc agttgcgata ccgtttatga
    23941 gacgtacaaa gattaaaaaa gctaaagatc catcaataaa ataaagtaat tgcgtgataa
    24001 ttaaagcaat taaaccaata aataataatc gtttaggtcc gatttgattt acaaatttac
    24061 ctgtagcaaa tcgaccaatt aaagacccga caataaatag tcctacaact aatccggcta
    24121 atgaatcgga agcgtcgaaa gcttgcttac tatagccagc aataactaca agtaacaaat
    24181 acatgcatag gtatacgata aagttgacta aaaagttaat tgtaaaactt ttggtgaaaa
    24241 taggtgattt atttaatgag ccttgattag tcattgtgtg cctcccttaa taatatttca
    24301 tgaatttgat tcatcacatt tatggtttct tgcatttgat cttgcgaaat gtttgttttg
    24361 gcaactactg cttgttggag cttttgtaca tcaagacaaa caatctcata taattcatgc
    24421 cctttaggtg ttaaagttaa aaacttttgt cgtttatcct ctaaactgtt ttctactgtt
    24481 ataaggtcat tttctattag agcttttaaa gtttttcttg cagtaggctt ttcaattgca
    24541 cgtctatgtg aaatttcaat gagagtagtg ggctgatgtt tagcgatatc gcgtaagatt
    24601 aaccattggc cataatatat attgtgtttt tctaaaatcg gctcggctaa ttttaaatag
    24661 gggcgatata ttgttataaa actattgaaa aattcttgtg atagcatagt gacatctcct
    24721 aagacaaaat agttagctta gctaaccttt ttacaacaat agtaattata aaacgggagc
    24781 aattagaaat caatatataa ttattaagag caaaaataat tatactttgt taaaataagc
    24841 gtaattacat gtaaataggg ggatactaat gatattgaaa tttgatcaca tcattcatta
    24901 tatagatcag ttagatcggt ttagttttcc aggagatgtt ataaaattac attcaggtgg
    24961 gtatcatcat aaatatggaa cattcaataa attaggttat atcaatgaaa attatattga
    25021 gctactagat gtagaaaata atgaaaagtt gaaaaagatg gcaaaaacga tagaaggcgg
    25081 agtcgctttt gctactcaaa ttgttcaaga gaagtatgag caaggcttta aaaatatttg
    25141 tttgcgtaca aatgatatag aggcagttaa aaataaacta caaagtgagc aggttgaagt
    25201 agtagggccg attcaaatgg aaagagatac acataaagat ggtaaggtaa agtggcaatt
    25261 gctttatata atgaatcagg atgatgatga aattaagcca ccatttttta ttcaatggga
    25321 agaaagtgat tccatgcgta ctaaaaaatt gcaaaaatat tttcaaaaac aattttcaat
    25381 tgaaactgtt attgtgaaaa gtaaaaaccg atcacaaaca gtatcgaatt ggttgaaatg
    25441 gtttgatatg gacattgtag aagagaatga ccattacaca gatttgattt taaaaaatga
    25501 tgatatttat tttagaattg aagatggtaa agtttcaaaa tatcattcgg ttatcataaa
    25561 agacgcacaa gcaacttcac catattcaat ttttatcaga ggtgctattt atcgctttga
    25621 accattagta taaatatacg taagtgctat gagcgagaat gcccatatga ataatgacaa
    25681 gcacaatgga aagaatcgtt aatatattat ttaatcgtga tgacttaatt aaaatgaaaa
    25741 agattgataa tataaatgtg aaaaagataa gtataacccg taaactaaag taattcacgg
    25801 tgagaggttg actcaatgtc ataatgattg caacgatgtt cataattata aatagactta
    25861 aaataattgt tctcatatca aacacctcat tgttagatta ttgacattat aacaggggta
    25921 attgtatatg aacattaatg tggttgcttg aggaaaaatt tattcattga agtcaagttg
    25981 gttcatttta gaaatgaata tcgtgttaga tgatgaaagt atattgaagt ataggtaact
    26041 agttgaaaag tattaattgt acgataacat taaatttaac acgaaacata gatataaaat
    26101 gattcacaat taaaatgggt aaatttgaac ttgctaaact attaattgga gcatggacat
    26161 ttcaaaaata agagttcaaa tcttacacaa gctctgaatc gacactataa gatacaaact
    26221 gtataattaa aggtattgtt aaatagaagg agatatcata aatcatggaa aagatgcata
    26281 tcactaatca ggaacatgac gcatttgtta aatcccaccc aaatggagat ttattacaat
    26341 taacgaaatg ggcagaaaca aagaaattaa ctggatggta cgcgcgaaga atcgctgtag
    26401 gtcgtgacgg tgaagttcag ggtgttgcgc agttactttt taaaaaagta cctaaattac
    26461 cttatacgct atgttatatt tcgcgtggtt ttgttgttga ttatagtaat aaagaagcgt
    26521 taaatgcatt gttagacagt gcaaaagaaa ttgctaaagc tgagaaagcg tatgcaatta
    26581 aaatcgatcc tgatgttgaa gttgataaag gtacagatgc tttgcaaaat ttgaaagcgc
    26641 ttggttttaa acataaagga tttaaagaag gtttatcaaa agactacatc caaccacgta
    26701 tgactatgat tacaccaatt gataaaaatg atgatgagtt attaaatagt tttgaacgcc
    26761 gaaatcgttc aaaagtgcgc ttggctttaa agcgaggtac gacagtagaa cgatctgata
    26821 gagaaggttt aaaaacattt gctgagttaa tgaaaatcac tggggaacgc gatggcttct
    26881 taacgcgtga tattagttac tttgaaaata tttatgatgc gttgcatgaa gatggagatg
    26941 ctgaactatt tttagtaaag ttggatccaa aagaaaatat agcgaaagta aatcaagaat
    27001 tgaatgaact tcatgccgaa attgctaaat ggcagcagaa gatgaaaaca tctgaaaagc
    27061 aagctaaaaa agcgcaaaat atgattaatg atgcgcaaaa taaaattgct aaaaatgaag
    27121 atttaaaacg agacctagaa gctttagaaa aggaacatcc tgaaggtatt tatctttctg
    27181 gtgcactatt aatgtttgct ggctcaaaat catattactt atatggtgcg tcttctaatg
    27241 aatttagaga ttttttacca aatcatcata tgcagtatac gatgatgaag tatgcacgtg
    27301 aacatggtgc aacaacttac gatttcggtg gtacagataa tgatccagat aaagactcag
    27361 aacattatgg attatgggca tttaaaaaag tgtggggaac atacttaagt gaaaagattg
    27421 gtgaatttga ttatgtattg aatcagccat tgtaccaatt aattgagcaa gttaaaccgc
    27481 gtttaacaaa agctaaaatt aaaatatctc gtaaattaaa acgaaaatag attaacgact
    27541 gaaatctgaa cgctcataag actgtcattt gcgttcagat ttttttacac aatatagaat
    27601 ggttgagtaa aatatttttg aatatagtga aagaggggga agtactgtga taaaaaagct
    27661 attacaattt tctttaggga ataagtttgc tatcttttta atggttgttt tagttgtctt
    27721 gggcggtgta tatgcgagtg ctaaattgaa attagaatta ctaccaaatg tacaaaatcc
    27781 agttatttca gttacaacaa caatgccggg tgcaacgcca caaagtaccc aagatgaaat
    27841 aagtagtaaa attgacaatc aagtaagatc attggcatat gtgaaaaatg ttaaaacgca
    27901 atccatacaa aatgcttcaa ttgtaacagt tgaatatgaa aataatacag atatggataa
    27961 agcagaagaa cagcttaaaa aagaaatcga taaaattaaa tttaaagatg aagttggtca
    28021 accagaatta agacgtaatt cgatggatgc ttttccggtt ttagcatatt cattttcaaa
    28081 taaagagaat gacttgaaaa aagtaacgaa agtactgaat gaacaattaa taccaaaatt
    28141 gcaaacggta gatggtgtgc aaaatgcgca attaaatggg cagacgaacc gtgaaatcac
    28201 ccttaaattt aagcaaaatg aacttgaaaa atatgggttg actgctgatg atgtagaaaa
    28261 ctatctaaaa acggcaacaa gaacaacgcc acttggattg ttccaatttg gtgataaaga
    28321 taaatcaatt gttgttgatg gtcaatatca atctgttgat gcttttaaaa acataaatat
    28381 tccattaacg ttggcaggag gacaagggca atctcaatcc caaagtgaca ataaacaaaa
    28441 ttcagccatg tcagacgtta atcaggcatc accacagcaa aattcaaaag cgtcagcacc
    28501 aaataatata agtggtatgc cgacagcgaa actaggagat ttagctgata ttacagttgg
    28561 tgatgtgcgt acttctattt ctaaaacgaa tggaaaggat gcggttaatc tacaaataac
    28621 taaagctcaa gatgccaata cagttcaagt agccaaagat gtacaacgta aaattgatac
    28681 attcgttgat gaaaataaag attttaatgt cacaaaaaca atggatactg caaagcctgt
    28741 tgagaaatca ctttatacga tggttgaaaa agcatcatta ggtacaatcg tggcaattat
    28801 agttattttg ctgtttttaa gaaacattcg tacgacggca atttctatta tatcgattcc
    28861 gttatcactt cttatggcgc ttattgctct gaaattgagt gatgtttcat tgaatatact
    28921 aacgttaggt gcattaacag tagcgattgg acgtgtgata gacgattcga ttgtagttgt
    28981 tgaaaatatt tatcgacgct taacagattc agaagaacaa ctaaaaggtg aaaatttaat
    29041 tatcagtgcg acaactgaag tatttaaacc aataatgtca tcgacactag ttactattat
    29101 cgtcttctta ccacttgtgt ttgtatcagg ttcagtaggc gaaatgttta gaccttttgc
    29161 attggctatt gcatttagtt tattagcatc gttattagtg tcaattacac tcgttccagc
    29221 gttggcagct acactattta aaaaaggcgt taaacgtcgt aataaacaac atcaagaagg
    29281 attaggtgtt gttagtacaa cttataaaaa agtattacat tggtcattaa atcataagtg
    29341 gattgtaatt atattaagta cattaatttt ggttgcaact attgtatttg gaggaccgag
    29401 actaggcact agctttattt cagcaggtga cgataaattt ttagctatta cttatacacc
    29461 gaagcctggt gaaacggagc aagcagtgtt gaatcatgcg aaagatgttg aaaaatattt
    29521 aaaacagaaa aagcatgtaa aaacaattca atactcagtt ggcggtagta gtccagtaga
    29581 tccaacgggt agtacaaata gtatggcaat catggttgaa tatgataatg acacgcctaa
    29641 ttttgatgta gaagcggata aggttattaa acatgcagat ggctttaaac atcctggaga
    29701 gtggaaaaat caagatttag gaacaggtgc aggtaataaa tctgtagagg ttactgtaaa
    29761 aggtccatca atggatgcca taaaatcaac tgtaaaagat attgaacaga aaatgaaaca
    29821 ggttaaagga ctagccaatg tcaaatctga tttatcgcaa acatatgatc agtatgaaat
    29881 taaagtcgat caaaataaag cggcagaaaa tggtatttct gcaagtcaac ttgcaatgca
    29941 cttgaatgaa aacttaccag aaaaaacagt tacgactgtt aaagaaaatg gtaaaactgt
    30001 tgatgttaaa gtcaaacaaa ataagcaaac agactggtca gaagataagt tgaataatat
    30061 aactttgaaa aagccgactg gtggtacgat taaattggga gatatcgcta cgttagttaa
    30121 aacaacgaca ccaagtaaat tgacgcaaga acaaggagat tatgcaacga cggtatctgc
    30181 taaagtaaca aataaagatg tgggtggcac aacacgacaa gtgatgtcta aaataaataa
    30241 tttggacaaa ccgaataatg taaaggttaa tatcggtggt gcatcagatg atattaacaa
    30301 tgcaatgaca caattagcct ttgcaatgtt agctgcaatc attatcgtat atttaatcct
    30361 agttattaca tttaaaggtg gcctagcacc atttacaatt ttattctctt taccatttac
    30421 agttatcggt gtaattattg cactattaat cacaggagaa acaatatcag taccaagttt
    30481 aattggtatg ctaatgttaa ttggaatcgt agtaacaaat gccattgtgt taatagaccg
    30541 tgttattaat aatgagcaac agggcatgga gatgaaagaa gcattaatcg aagcaggcgg
    30601 tactagaatt agaccgatat taatgacggc gattgcaaca attggtgcat tagttccttt
    30661 gttatttggt caagatagct cgattcttat ttcgaaagga ttagctgcta cagttattgg
    30721 tggtttaatt tcatcaactt tattaacact tgtagttgta ccagtaatat atgaaattct
    30781 ctttacttta aaaaaacgat tcactaaacg atagataata taaaagccat aaaagcggtc
    30841 atgatattga ctacttttat ggctttttaa aatttgaaat tttagacctt atttcacgtg
    30901 cgatttaatc gatgtgttaa taaaacaata ctggcattca acatgtaatc cgataataaa
    30961 ataaggttgc aattatgtta ttgcatattt tgtttattac ttagaataat acgatggtta
    31021 agatgaaata tatgacaaag ataaataatg acattaaatt caagatgttt acgcctttta
    31081 gatctttttt aagcatttta aatcctgtca cttcgacatt accgactaag agtaataaga
    31141 cgataaatac ccaccaattc tcttttggaa aactcatcgt cgttaatgct aagacgaata
    31201 ataacaacac acccataata attctcaaaa tgcgagtaaa tacaacttcc attttaaaaa
    31261 tactaataaa actaacaatt agataaagta ttgctagtaa aatcagataa aattgcatat
    31321 gataacttcc tctccgtttc aaagggttca tgtagtacag cagcgtagaa aacataaata
    31381 gcagtgttgg gatagatatg cgtgtatgtt gttaatgaaa cactaaatca taacacgaca
    31441 acatcttgtg attctagatg taattgagcg aaaaatgaag caattcatca atataacgta
    31501 ttcagagcaa tgctagtcaa atttatgtaa caaaaagcat ttattgcttg aatgatagat
    31561 tattaatctt gcatttgagc acgaacgttt ctaatttcat tttcaatatc tttaagttga
    31621 cgaacaagtg cggaagtatc taaattcaaa gattcccgtt tttctatgtc accttgaaga
    31681 cgaacataat catatttaag ttcggctagt tggttttcta aagacatact ataacactcc
    31741 tttgaaatat aattaagtgc atttaatgta gattatagca tgaaacagta gacttctaaa
    31801 atatctgtgg agtatgttaa agttataaat tatgaagatt attgaaagaa gggataacag
    31861 tgacagaaca atttactatg attattttat taattgcgct tggttacttt ttgaagcgca
    31921 ttaattttat aaaagcaaca gatagtcaag tcattgctac attagttctt aatgtcacac
    31981 tcccatcttt agttattgtg aatttaaaca gtgcagaatt aaaattgtca ttctccattt
    32041 taccaatttt aatgattata tatggtattg tggcgaaaat gattgctgtc tggtttttta
    32101 gaaaatatga taatcacatg cgtggatcag taggtatgat gactggtgct atgaatattg
    32161 gattgtttgc atatccacta attgaagtga tatggcctaa aacgggactt atttattttg
    32221 ttatggctga tataggtggt gcctttgtga tgtttggaat tacttacttt gtaggtagct
    32281 actttagtga aggtagtgat caattcgatt ttaaatattt aggtaagaaa ttattacaat
    32341 cagtgccact cgttacatac attgtaatgt ttatattgaa tataagtcac atccatttac
    32401 cacatgtggc aatcgatttc tttagcatac tttcccatgc aaatatgccg ttatcaatga
    32461 ttttgctagg tgtcatgttg aattttactc tggaaagaaa gtatatacct gctactatta
    32521 aatatttatg tttgcattat ggattagcat tagttgcggg attgttagta tatgtgttgt
    32581 taccagtatc agatgatatg attaagacga cattgttggt cacttggatg ttcccggtag
    32641 gtgttgcaat tatttcttat ggtattcagt ttaaatatcg aacactgaca tttataggta
    32701 tgacgaccaa tttgacaatc attattagta tcattatttt gtacgtgtat caggcagtgt
    32761 ttgtctagtt tacacatttg aaaataaaat ccattttagt attcgtagaa aatgagagcc
    32821 ccctttacca caatatgtaa atgtatattg tgaaaagggg gcttttattt atgacatagt
    32881 tgttcaactc atacattcaa gttgataatg cagtgcgtta tgcagctatc cacttcatat
    32941 gtatggcgca tacttgatat atgaatatca tcaagatttt aaaagtatcg ctaattttaa
    33001 agtcttaaaa cgcatgatta atgtcatgat gtgttattgc gttttgtttt tagagccacg
    33061 tatttcctag gacatataga ggaacattca acgtaaaatt cccgaaaatt aattttaatc
    33121 attctataag tgaatggtta ttttcagaag tgattacatt tttacgttac atgagtattt
    33181 aactttctat ttcaaattac ctagaattat agcagctatc acgataataa taatacctgc
    33241 ccaaataccc gtcatctgac gacgatcttt tctttctcct aaaatgaaaa taccgcctaa
    33301 ggttgaaacg ataacaagta actgtgataa tgagaaactt gtagctacac caacttttgg
    33361 ttgagaatag aacatgaaca agttaccaat tccccaaatc acacctggca atagattaag
    33421 tgctgttgat ttaagtgatg ttttatgatt catggatagg ataaagccac caattgccat
    33481 accgacagat tggaagaaca atgcatcagt tccaccaaca ccaaagatgt caccaagtac
    33541 aacgaaacct acatatccaa ctgtagatac aattaaaata cccattgctt ttttaaattc
    33601 aggattatct gattgacgtt catttttagc tttaagtgaa gtaagtgcta caccagtaac
    33661 taataatatc atggcgatta aaccaaagat aatttgagtc attgaagacc attcacctaa
    33721 aaagataacg ctgaataatg ttgtaccaac taattgcata cctgtagaaa ctggcatagt
    33781 atttgataca ccgattaaac taatagattt aagctgattc gcttgtccaa acgcccataa
    33841 tgcaccagaa ataagaccga caataattac agtaggatta ttgaattttg cgaaaccagt
    33901 aataagtaaa cctaatccaa caatgagtgc gcctagcgtc gtaccacgaa tttggttgta
    33961 aggtccaccg ccgacgaaca cattaataag aacgacactt ccccagaata aagcaggtaa
    34021 aagtgcgatt aagaaatcaa gaaattgcaa gactactcac tctcctatac tatttatgca
    34081 agtgctcccc ttaattgaca cttgcatgtt attgtaaagc attctgccct aatagtaaaa
    34141 aacaactact tataattttc aatatttaca aaaagtagaa aaacccacaa tacatgctaa
    34201 tgatgtactg tagggtattt gcttggtgtt ttattcaggg attgtttgat cttttacgtt
    34261 acgtgattcg aatttgaatt taatgtactc atagttgata tggaaaccta gaccattaaa
    34321 tcgttcgatg agtttattat ttttattacg aattttaaaa taaacttttc taatatcaaa
    34381 attatcaaaa gcatattgca atgcatacgc aagcaactcg aaagcgatac ctttcaaacg
    34441 gtaatctaca tgagaactga agtatttaat ctcggcgatt gactgttgtg aatcaatttc
    34501 aaggtataaa taacctttaa gtaaaccttc gctaacaaat aaaaacaaat gatgatggtc
    34561 gtctaaacta tcaatgatat cttgtggtga ctgtgcctga tatttaaatg tagttgtgtg
    34621 taatttgctg aaagcacgat aaaacccttt gtgataagga atgatatttg gttggtgcat
    34681 atcttcttct aatcttgtac gggcttctat gtaatagtca gtgaagttat aacttgcttg
    34741 aataactttc attaatggtt tgtattgttg aatgccttct tcaaaagaaa agttaaagac
    34801 ggcatcgtca ggttggttcg aagtcattgc tttaaatagc gtttcaaacg tatcgacaga
    34861 taatacataa ttgtcagcca cgaaagggcc tatgactttg aatttatttt tatcgtatgc
    34921 aaaagacaat atgattgctt tgattgaatc gttttcttta atcgaaaata caccaggtga
    34981 ttcaatcgca tcggttatta atacatctat ttcattgtac tgttgaggaa gtttatataa
    35041 gtatgatgct gattcataat tcgaatgttc aatgaacgat tttatttgtg taatatcata
    35101 taactgaact atgctcatgt cattacctcc gtactttttg ttacttttat tatatagtat
    35161 ttcaactgaa atgaaagtta atagtgatat taacatgtta caatacattt aacacatcat
    35221 ttgcaatcat tgaatttaaa tcaaagatta gtggaataga tgaagcacgt tcgaaataaa
    35281 agaacgtatg agaaaggata atttatgaaa tctttaatat tagctgaaaa accatcagtc
    35341 gcaagagata ttgctgatgc tttacaaata aatcagaagc gtaatggtta ctttgaaaat
    35401 aaccaatata ttgtcacgtg ggcgttaggt catctagtga caaatgcgac acctgaacaa
    35461 tacgataaaa atttaaagga atggcgatta gaagaccttc caattatacc taaatatatg
    35521 aaaactgttg ttattggtaa aacaagcaaa caatttaaaa cagtaaaagc gttaatttta
    35581 gataataaag tgaaagatat tattattgca acagatgctg gacgagaagg tgaactagtt
    35641 gcaagattga ttttggataa agttggtaac aaaaagccaa tccgtcgatt atggattagc
    35701 tcagttacta aaaaagctat tcaacaaggt tttaaaaatt taaaagacgg tcgtcaatat
    35761 aacgatttgt attatgcagc gttagcgaga agtgaggcag attggattgt tgggattaat
    35821 gcaacgcgtg cactaacaac aaagtatgat gcacagctat ccctgggacg tgttcagaca
    35881 ccaacgattc aattagtaaa tacacgacaa caagagatta atcagttcaa accacaacaa
    35941 tactttacat tatcattaac ggtaaaaggg tttgattttc agctagaatc aaatcagcga
    36001 tataccaata aagaaacttt agaacagatg gttaataatt tgaaaaatgt cgatggtaag
    36061 attaaatctg ttgctactaa acataagaag tcgtatccgc aatcactgta caatttaaca
    36121 gatttacaac aagatatgta tagacgttat aaaattggac ctaaagaaac attgaataca
    36181 cttcaaagct tatatgagag acataaagtc gtaacctatc caagaacaga ttcaaactat
    36241 ttaacaactg atatggtaga tactatgaaa gaacgtattc aggcgacgat ggcaacaaca
    36301 tataaagacc aagcacgccc attaatgtct aaaacatttt catcaaaaat gtcgatattt
    36361 aataatcaaa aagtatctga tcaccatgca attattccta cagaagtgag acctgtcatg
    36421 tcagacttaa gtaatagaga attaaagtta tacgatatga ttgtcgagcg ttttttagaa
    36481 gctttaatgc ctccgcacga gtatgacgcg ataactgtaa ctttagaggt tgcagggcac
    36541 acatttgttt tgaaagagaa tgtaacaact gttttaggtt ttaaatctat tagacaaggt
    36601 gaatctatta cagagatgca acagcctttt tcagaaggcg atgaagtgaa gatttcaaaa
    36661 acaaatatta gagaacatga aacaacacct ccagaatatt ttaatgaagg ttcgttatta
    36721 aaagcgatgg agaaccctca gaactttatt caattgaagg ataaaaaata tgcgcaaact
    36781 ttaaaacaaa caggtggtat cggcacagtt gcaacaaggg ccgacattat cgataaatta
    36841 tttaatatga atgccattga atcaagagac ggtaaaatta aagtaacgtc aaaaggtaaa
    36901 caaatattag aattagcacc agaagaatta acgtcgccac ttttaactgc acaatgggaa
    36961 gaaaaattac ttttaattga acgtggtaaa tatcaggcga aaacatttat taatgaaatg
    37021 aaagatttta cgaaagatgt tgtaaatggg attaaaaata gtgatcgtaa atataaacac
    37081 gataatttaa caaccacaga atgcccaacg tgtggtaaat tcatgattaa agttaaaact
    37141 aaaaatggtc agatgcttgt gtgccaagat ccatcttgta agacgaaaaa gaatgtacag
    37201 cgcaaaacaa atgcaagatg tccaaactgt aaaaagaaat taacgttgtt tggtaaaggg
    37261 aaagaagcgg tatatcgttg tgtttgtgga cattctgaaa cgcaagcaca tatggatcag
    37321 cgtatgaagt ctaaatcctc tggtaaagta tctcgtaaag aaatgaaaaa gtatatgaat
    37381 aaaaatgaag gtttagacaa taatccgttt aaagatgcat taaagaactt gaatttatag
    37441 ataaaatcga acaaagttga atcagaaaaa cgaaaagttc gcttttggta ttgtttttta
    37501 ttaagaatga tattaaacta ttaaggtatt ttaaaaaaag gagcatccat tcgtgaaaaa
    37561 ctatttccag ttcgataaat atggaacaaa ctttaaaaga gaaatcttag gcggtatcac
    37621 aactttctta tctatggcct atattttagc agttaacccg caagttttaa gtttagcagg
    37681 tgttaaaggc gtatcagaag atatgaaaat ggaccaaggt gccatttttg tagcgactgc
    37741 attagcagca tttgtaggct cgctattcat ggggctaata gctaaatatc caattgcgtt
    37801 agcaccaggt atgggattaa atgcattctt tgcatttact gttgtgttaa ccatgggtat
    37861 tccttggcaa gttggtttaa caggcgtttt attctcaggt atattctttg cgatattaac
    37921 cgtcacaggg tttagggaag ttattattaa tgcaattcct tatcaaatga agatggctgt
    37981 ttcagcaggt ataggattat tcattacatt tgtaggttta caaagttcgg gtattatagt
    38041 taaaaatgaa tctacattag taacattggg acatttaact gatccaccag tacttttggc
    38101 gatattcggt atcgttatta cagttatttt atatgcaatt aaattgccgg gttctatctt
    38161 tatcggtatg atcatcacag caatcgttgg tatgttcact ggattgattc aaatgccatc
    38221 tggtattgtg ggtaagattc caagtattga accaacgttc ggtgctgcat ttgaagcgtt
    38281 taaagaccca agtcaattat taacgataca gttcttaatt gttattttaa cgttcttatt
    38341 tattgatttc tttgatacag ctggtacatt agtagctgtt gctactcaag caggtattat
    38401 gaaagataat aaattaccaa gagctggtag agcattgttc tcagattctt tagctacgat
    38461 tgtaggatca gtttttggaa caactacaac aacttcttat atcgaatcaa cttcaggtgt
    38521 tgctgtaggt gctagaacag gatttgctag tatcgtaaca ggttgttgtt tcctattagc
    38581 gctgttcttt agtccgttaa tcgctgttgt aacaagtgca gttacgacac cagccttagt
    38641 ggtggtaggt gtattaatgg ctgcaaactt tgctgagata aactggaaaa gttttgaagt
    38701 tgcagtgcca gccttcatta caatcattat gatgccttta tcatattcaa ttgcaactgg
    38761 tattgcgtgt ggatttattt tctatccaat tacaatgctt atctctaaaa aacataaaga
    38821 agtacatccg ataatgtatg tattaatggt actattcatt ctgtacttca t
//
LOCUS       DANJFZ010000012        38071 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.012, whole genome shotgun sequence.
ACCESSION   DANJFZ010000012 DANJFZ010000000
VERSION     DANJFZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38071
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.012"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(86..265)
                     /locus_tag="PWN45_000506"
     CDS             complement(86..265)
                     /locus_tag="PWN45_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDL0622295.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            416..1414
                     /locus_tag="PWN45_000507"
     CDS             416..1414
                     /locus_tag="PWN45_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0622296.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1646..2527
                     /gene="rbsU"
                     /locus_tag="PWN45_000508"
     CDS             1646..2527
                     /gene="rbsU"
                     /locus_tag="PWN45_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDL0622297.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2542..2946
                     /gene="rbsD"
                     /locus_tag="PWN45_000509"
     CDS             2542..2946
                     /gene="rbsD"
                     /locus_tag="PWN45_000509"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL0622298.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2974..3888
                     /gene="rbsK"
                     /locus_tag="PWN45_000510"
     CDS             2974..3888
                     /gene="rbsK"
                     /locus_tag="PWN45_000510"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL0622299.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3986..4120)
                     /locus_tag="PWN45_000511"
     CDS             complement(3986..4120)
                     /locus_tag="PWN45_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622300.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            4139..4900
                     /locus_tag="PWN45_000512"
     CDS             4139..4900
                     /locus_tag="PWN45_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0622301.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5334..6770)
                     /locus_tag="PWN45_000513"
     CDS             complement(5334..6770)
                     /locus_tag="PWN45_000513"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0622302.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6786..7577)
                     /locus_tag="PWN45_000514"
     CDS             complement(6786..7577)
                     /locus_tag="PWN45_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0622303.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7726..8430
                     /locus_tag="PWN45_000515"
     CDS             7726..8430
                     /locus_tag="PWN45_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0622304.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8538..9239)
                     /gene="lrgB"
                     /locus_tag="PWN45_000516"
     CDS             complement(8538..9239)
                     /gene="lrgB"
                     /locus_tag="PWN45_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDL0622305.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9232..9675)
                     /gene="lrgA"
                     /locus_tag="PWN45_000517"
     CDS             complement(9232..9675)
                     /gene="lrgA"
                     /locus_tag="PWN45_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDL0622306.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9788..10528)
                     /locus_tag="PWN45_000518"
     CDS             complement(9788..10528)
                     /locus_tag="PWN45_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDL0622307.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10531..12285)
                     /locus_tag="PWN45_000519"
     CDS             complement(10531..12285)
                     /locus_tag="PWN45_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0622308.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12532..13206)
                     /gene="scdA"
                     /locus_tag="PWN45_000520"
     CDS             complement(12532..13206)
                     /gene="scdA"
                     /locus_tag="PWN45_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDL0622309.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13346..15070)
                     /gene="tarS"
                     /locus_tag="PWN45_000521"
     CDS             complement(13346..15070)
                     /gene="tarS"
                     /locus_tag="PWN45_000521"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL0622310.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(15103..16791)
                     /locus_tag="PWN45_000522"
     CDS             complement(15103..16791)
                     /locus_tag="PWN45_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL0622311.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16813..17838)
                     /locus_tag="PWN45_000523"
     CDS             complement(16813..17838)
                     /locus_tag="PWN45_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL0622312.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17831..18547)
                     /locus_tag="PWN45_000524"
     CDS             complement(17831..18547)
                     /locus_tag="PWN45_000524"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDL0622313.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(18823..19992)
                     /locus_tag="PWN45_000525"
     CDS             complement(18823..19992)
                     /locus_tag="PWN45_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HDL0622314.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(20420..22114)
                     /locus_tag="PWN45_000526"
     CDS             complement(20420..22114)
                     /locus_tag="PWN45_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL0622315.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(22136..23161)
                     /locus_tag="PWN45_000527"
     CDS             complement(22136..23161)
                     /locus_tag="PWN45_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL0622316.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(23154..23870)
                     /locus_tag="PWN45_000528"
     CDS             complement(23154..23870)
                     /locus_tag="PWN45_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL0622317.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(24398..25441)
                     /locus_tag="PWN45_000529"
     CDS             complement(24398..25441)
                     /locus_tag="PWN45_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL0622318.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(25465..25611)
                     /locus_tag="PWN45_000530"
     CDS             complement(25465..25611)
                     /locus_tag="PWN45_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622319.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(25613..26668)
                     /locus_tag="PWN45_000531"
     CDS             complement(25613..26668)
                     /locus_tag="PWN45_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDL0622320.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(26686..27945)
                     /locus_tag="PWN45_000532"
     CDS             complement(26686..27945)
                     /locus_tag="PWN45_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL0622321.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(28172..28450)
                     /locus_tag="PWN45_000533"
     CDS             complement(28172..28450)
                     /locus_tag="PWN45_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0622322.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(28473..28940)
                     /locus_tag="PWN45_000534"
     CDS             complement(28473..28940)
                     /locus_tag="PWN45_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0622323.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(28925..31021)
                     /locus_tag="PWN45_000535"
     CDS             complement(28925..31021)
                     /locus_tag="PWN45_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL0622324.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(31152..31250)
                     /locus_tag="PWN45_000536"
     CDS             complement(31152..31250)
                     /locus_tag="PWN45_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622325.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            complement(31360..32295)
                     /locus_tag="PWN45_000537"
     CDS             complement(31360..32295)
                     /locus_tag="PWN45_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL0622326.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            32657..34186
                     /locus_tag="PWN45_000538"
     CDS             32657..34186
                     /locus_tag="PWN45_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0622327.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(34506..35459)
                     /locus_tag="PWN45_000539"
     CDS             complement(34506..35459)
                     /locus_tag="PWN45_000539"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0622328.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(35770..35877)
                     /locus_tag="PWN45_000540"
     CDS             complement(35770..35877)
                     /locus_tag="PWN45_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622329.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            36032..37177
                     /locus_tag="PWN45_000541"
     CDS             36032..37177
                     /locus_tag="PWN45_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDL0622330.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            37203..37376
                     /locus_tag="PWN45_000542"
     CDS             37203..37376
                     /locus_tag="PWN45_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622331.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            37533..37889
                     /locus_tag="PWN45_000543"
     CDS             37533..37889
                     /locus_tag="PWN45_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDL0622332.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
BASE COUNT        12045 a         7186 c         5745 g        13095 t
ORIGIN      
        1 actaataatc tcttcttctt ttaatttgaa ttgcatttaa aaacatcctc tcttaagttt
       61 taacaagcct taattaaaaa ttttttcaat cacatagttc aatatacatc atttcgttat
      121 gttttttaat actttgttca aaaacaaata ttttattctt taaaataatg acttttgtat
      181 ttttaatcac aataaacatt ttaaaattct tgttatcata atcattaaaa ggtattaacc
      241 ttaataatat attctctcgt ctcaacctta atcgtatact tcagacgtct gtttgtagac
      301 aataaaagtc attcacgtct tcatatgtca tcaaatgttt atcatgatat gatgaatata
      361 ataatcgggt atataactgt atgattaatt acacaataaa gagaaggaat ataatatgaa
      421 aaaagtgtca attaaagatg ttgctagaga agctggtgta tcagttacaa ctgtgtcaca
      481 tattttaaat cataatgata gtcgtttttc cgcaacaacg ataaaaaacg tacatgctgt
      541 ttcagaacgt ttaggctatg cccctaataa acatgcaaaa caattgcgcg gcagtaaaat
      601 tcaaactatt ggcgtcattt tgcctagctt aacaaatccg tttttctcag cactgatgca
      661 aagtattcat gaccataaac catctgatgt tgatttatgc tttttaacat ctacagcaac
      721 tgatttgtat gacaatatta aacatttaat tgatcgaggt attgacggat taattatcgc
      781 acaatacata tcatccccgg acgccctaaa taactatcta aagaaacatc atgtacctta
      841 tgtcgtactg gatcaaaatg accatcaagg ctatacagat tttgttcgga caaatgaata
      901 tcaaggtgga caacttgcag cacaacattt aatagaactc ggtcacaacc atatgataat
      961 tgttgcacca tatgacatga tggcgaatat gtcgactcgt gtcgctggat ttgtcgatac
     1021 tttgcgcgcg aatcaattgc cagaaccaca aatcgtccat actgaattat ctaagcgcgg
     1081 tgggctaacc attgttgatg acatcatggt tcaatctgcc actgcaatct tcgctattaa
     1141 cgatgaactc gctattggca ttttacgagg actaattgaa catggcatca gtatcccgaa
     1201 agatatctca ttaataggtt atgacgacat tgattatgca gcgtacgtct cgccaccttt
     1261 aactactgtg gcacaaccta taactgatat tggcaaaaca tctttaacct tattacttca
     1321 acgattacag cacttagata aatccattga tatgattgaa ttaccaacga ctttaaaaat
     1381 tcgtgcaaca actggctatc atctttcaaa ctaactacgt atcttccgaa atatactcat
     1441 cattgttagg cccttagcgt tgctttaatg ctgagggttt ttaatcataa ttattttact
     1501 aagaaattaa aataataatg tatgaatttt taaatatgat ttaaacgttt tcagttttta
     1561 tgaaaacgca tgcattttac aaataaaaat ggtacgatgg cactggtaaa acgttttact
     1621 aaaaacaaat catgaggtgt ataacatgag cattgttgca ttacttatcg ggttaggccc
     1681 cttaattggc tggggcttct tcccaacagt cgcttcaaag tttggtggta aacctgtaca
     1741 tcaaattatc ggtgctactg taggtacgtt aatcttcgct attatattag ccgtagtcac
     1801 atcaagtggc ttacctactg gaaccaattt gctattcgcc ttattatcag gtgcaggatg
     1861 gggattcgga caaatcatta catttaaagc gttcgaatta gtcggctcat ctcgtgccat
     1921 gccagtcaca acagcattcc aattattagg cgcatcttta tggggtgtct ttgcattagg
     1981 aaattggcca ggcattggtc ataaaatcat tggatttaca gctttagtcg ttattctaat
     2041 tggagcgcgt atgacagttt ggagtgaacg caaagaagca agtaacgcca aaaatttacg
     2101 tcgtgcagtg gtacttctgt taattggtga atttggatac tggttatatt cagctgcacc
     2161 gcaagcaact tctattgatg gcctaactgc ctttttacct caagcaatgg gtatggtaat
     2221 tgttgcagtc atttatggct ttatgaatat gaaagcagag aatccattcc gtaataaaat
     2281 tacgtggtta caaattattt caggtttctt ctttgcattt ggtgctttaa catatcttat
     2341 ttcagcacaa cctaatatga atggtttagc aactggattt attctttctc aaacatccgt
     2401 tgtgcttgct acattaactg gtatttattt cttaaaacaa cataaaacgt caaaagaaat
     2461 ggttattaca atcatcggct tagtactcat tttagtagcc gcttctgtta cagtatttat
     2521 aaaataagga gtgtagatgt catgaaaaaa tcagctattt taaatgaaca tatttcaaaa
     2581 gcaatcgcga caattggtca ttttgattta ttaacgatta atgacgctgg catgccaatt
     2641 ccaaatgatc atcgtcgtat cgacctagct gtaactaaaa acttaccacg ctttattgat
     2701 gtcttagcta cagtgttaga agaaatggaa atccaaaaaa tatacttagc agaagaaata
     2761 aaagaacata accctacaca attgcaacaa attaaacaat tgatttcatc ggaaatcgaa
     2821 atcattttca ttcctcacga agaaatgaaa agtaacttag ctcacccatt aaataaaggt
     2881 aatattcgta ctggtgaaac aacgccctac tctaatattg cattagaatc gaatgttact
     2941 ttttaaaagt tataacttga aaggagcgta cacatgacca acaaagttgt tattttaggt
     3001 tcaacgaatg tcgatcaatt tttaacagtt gaaagatatg cacaaccagg cgaaacatta
     3061 catgttgaag aagcacaaaa agcattcggc ggaggtaaag gtgccaacca ggctattgcc
     3121 actgcacgca tgcaagcaga cacaacattt attactaaaa ttggcactga tggcgttgct
     3181 gatttcatct tagaagattt taaagtagct catattgata catcatatat tatcaaaaca
     3241 gctgaagcaa aaacgggcca agcctttatc actgtgaatg cagaaggaca aaacaccatc
     3301 tatgtttatg gtggtgcgaa tatgacgatg acacctgaag atgttattaa cgcaaaagac
     3361 gctataatca atgcagactt tgtcgttgca caattagaag tacccatccc ggctattata
     3421 tctgcatttg aaattgccaa ggcacatggt gtgacgacag tattaaatcc tgcaccagcg
     3481 aaagcattac ctaatgaatt attatcatta atcgatatta ttgtgccaaa cgaaacagaa
     3541 gccgaattgt tatctgggat taaagtaact aatgaacaat ctatgaaaga caatgccaat
     3601 tactttttat ctataggcat taagactgtt ttgattacgc taggtaagca aggtacatat
     3661 tttgctacta aaaatcaaag ccaacacatc gaagcttata aagtaaatgc gattgataca
     3721 actgctgcag gcgacacatt tattggtgca tttgtcagtc gcttaaacaa gtcgcaagat
     3781 aacttagcag atgctattga ttttggtaat aaagcgagct cactcactgt acaaaaacac
     3841 ggcgcgcaag catctattcc tctactagaa gaagtaaatc aagtttaaat gaatcaaaca
     3901 cagctatgat atgaaggttt agcatataac atgcaacatt cgtatatcat ggctgtgctt
     3961 ttttatcttt ataaaacatc atctattaga aataattttc cactaaacct atgcttgttg
     4021 actcatgttt agttataaat gaagtgacaa ttttttgtaa tctttttaac ttccaaatta
     4081 tcgcatataa atatgctata ttaatgataa taattatcaa ttaaaaggag gttatgctat
     4141 gtctaaagaa gctggtcata catttttagc taaattagga aaaactcgtc tacgccccgg
     4201 tggtaaagaa gcaacagatt ggttaataca acaaggggca ttttcacaag ataaacaagt
     4261 gttagaagtg gcatgtaata tgtgcacaac atctatttat ctagctcata catatggctg
     4321 tcacattcaa ggcgttgata taaataagaa agcattagaa aaagcacagg aaaacatttc
     4381 agcagcaggt cttgaatcat atattcaagt tcaacaagcg aatgctgtta aattgccctt
     4441 tgatgacaat caattcgata tcgttttaaa tgaagcaatg ttaacaatgt tacccatcgc
     4501 cataaaggaa aaagcattac gcgagtacta ccgagtctta aagcctgggg gtatcttgtt
     4561 aacacatgat attgtcatcg ttaatgaatc acatgccaca catgttgtta aatcattatc
     4621 tgcagcaatt aatgtcaatg tctcaccgca gacgaaactt ggctggttag atttatataa
     4681 tcaagctggt tttaatcatg tgcattatca tactggtcca atgagtttaa tgacaccaaa
     4741 aggtttaatt tatgacgaag gtattgttgg aactataaag attatcaaca atgctttgaa
     4801 aaaagaaaat cgaccaatgt tttgtaaaat gtttaaaacg atgactaaat tgcgtaaaga
     4861 tatgaattat attacttttg tcgctaaaaa agagcactaa atataatgcc actaactgta
     4921 ctttgtatct atgtttgact atcactttaa tttctttgtg acactaatca tctacttaac
     4981 aatatcgtta tcgttgatta gtaagtcatc aattttggtt aaagactttc ataaacactc
     5041 aaacattaac actatacata gttagtggca ttattttttt cttaaaattt taacatcacg
     5101 ggattggaac agaaatgata ttttcgcaaa atttatttct gtccttcccc aacttgcatt
     5161 acctgtagaa tttcttttcg aatttctctg tgttggggcc ccgccaactt gcattacctg
     5221 tagaatttct tttcgaaatt ctttgtgttg ggggcccgcc attataacac tgtaggtcct
     5281 actatattaa tttatgtctc gggctctaac cctaaaatac ttataatata cctttataat
     5341 ttatctccat ttgatgctat cacttgttga taccaataga acgaatcttt tttcaagcgt
     5401 tccatcgtgc catgaccatc attatctcga tcaacatata ttaaaccata gcgtttcttc
     5461 atttcaccgg ttgtaaatga aacaatatca atgattcccc acggtgtata accgattaaa
     5521 tctacaccat cttgatcaac tgcttctatc gctgctgtaa tatgtgcttt taaatattca
     5581 atgcgataat catcatgaat atgtccatct accacttcat caactgcacc aaaaccattt
     5641 tccacaataa aaagtggtaa ctgataacga tcatataaca cattcaatgt atatcttaag
     5701 ccatctggat caatcgccca accccaatca ctcgttgcga tatgcggatt ctccacagaa
     5761 tgattcaaac caccgttgac gatgttgttt tcaactgtag tatcaacatc atgttttaca
     5821 gccgtagaca tgtaataact aaagccaata taatcaaccg tgccctgctg taaaatactg
     5881 tcgtcccctt cttgccatcc aacatcatat ccttttcgtg ccaacatttt tttagcatag
     5941 cttggataat aacctctcac ttggacatcc gggaaaaaga aacgtaagcg attcgcaatt
     6001 tgtgcttcca tcatatcttt cggatgacac gaatatggat aaatgggtac atgtgaaatc
     6061 attgttccaa tcttaaactt cggattaatc tctttaccaa gacgaactgc taaagcactg
     6121 gctaaaagtt catgatgtgc tacttgatac aagacttctt caggattatc attttctgtc
     6181 aatgctaccc cagaattcgt ccataaaaag ataggatttg atgtgtccat ctgattatta
     6241 atttcattaa acgtcatcca atatgtaact ttatctttat atctttcaaa tacaacacgc
     6301 gcaaaatgca caaaataatc gacaacttct ctatttctaa atccaccata atgtttcgct
     6361 aaatgaagtg gcatctcaaa atgtgataac gtcacaacag gttcaatacc ttgtgcaatt
     6421 aattcatcaa agatacgatc ataaaaggcg agtccttctt catttggcac atcttcatcc
     6481 ccattcggaa agatacgtgt ccacgcaatc gacgtacgta aacatttcaa tcccatttct
     6541 ttaaacaagg caatatcttc cttataacga tgataaaaat caataccttc atgatttgga
     6601 taatagtgat tgggatctat agattctgta atctgacgtg ctttgccatg tgcaccactc
     6661 gtcataacat caattacact taacccttta ccacctttat catatccacc ttcaaattga
     6721 tttgcggcaa gagcgccacc ccacatgaaa ttttgcggta attttgtcat tattacaaca
     6781 cactcctagt ttttaaatat tttaaaaata acatcttcag tcgtaatgat ttcattcatt
     6841 gtcagttcta cttttcctaa atcggcagag ttgctaataa cgacaataac gtcagcattg
     6901 tagccttgtt gttgtatata ttgctggtcg aattgcaaca acgtttgccc tgcttcaaca
     6961 tgatcatttt gctttacaaa gcacttgaac cctttacctt ccaagtcaac tgtattcaag
     7021 ccaatatgaa tgactatgtc cacaccgtct tctgattgaa taccaactgc atgcttagtt
     7081 ggtacaatca tagatattaa accattgaac ggtgcgatta ctttggattc ttcatgagct
     7141 ttgattgcta agccttcacc taccattttc tctctaaaaa ttgaatctct tacttcttta
     7201 agtaaaatac gacgaccagc gattggtgct ttcactaaaa ttggtgttgt tacttttact
     7261 tgaggtgtat aacttgtatc ttgactatta atattgtcag tattctcact ctcttcgaca
     7321 ttagcattct taattaatcg ctgtaattgc tgatacactt catcgacatt tccatttaat
     7381 tttatgacta actgcgtatc attttctgct acagatgtca catcaactat ttgtttcact
     7441 tcatttgctg tagaaggaat tgtatgttgc atatgaattg taatgcactt cgtatcatac
     7501 gttgcatcaa caatgttttc gacaccacct acagctgtaa gaatgtcttg ggcttgttct
     7561 ttatatttat tgctcataaa tattaccccc tcaacttgat agtagaatga aaatgtattt
     7621 acttacctcc aattataatt gtaccggttc aatttgtaaa cgccgataca attataatat
     7681 tttgtgctat aataattaca gacaaagtga aacgaggaca gaatattgtt aaagtatgaa
     7741 catattgcta agcaacttaa tgcgtttata catcaatcta atttcaaacc cggtgataaa
     7801 ttgccaagcg tgacgcaatt aaaagaacgt tatcaagtaa gtaagagtac tatcattaaa
     7861 gcattaggct tattggaaca agatggtttg atctatcaag cacaaggcag tggtatttat
     7921 gtgagaaata ttgctgatgc caatcgtatc aacgtcttta agactaatgg tttctctaaa
     7981 agtttaggtg aacaccgaat gacaagtaag gtacttgttt ttaaggagat tgcaacgcca
     8041 cctaaatctg tacaagatga gctccaatta aatgcagatg ataccgtcta ctatttagag
     8101 cgattaagat tcgtggacga tgatgtttta tgtatcgaat attcttatta tcataaagaa
     8161 atcgtgaaat atttaaatga tgatattgct aagggctcta tcttcgacta tttagaatca
     8221 aacatgaaac ttcgtattgg tttttcagat attttcttta atgtagatca actcacttca
     8281 agtgaagctt cattactaca attgtctaca ggtgaaccat gtttacgtta ccaccagact
     8341 ttttatacaa tgactggcaa accctttgat tcatctgaca tcgtatttca ttatcgtcat
     8401 gcacagtttt atattcctag taaaaagtaa taaatacata aaaacgtcta tatcccagtt
     8461 ataaactgga gtatagacgt ttttttacga taataacaat ggctcaaatt gctattatct
     8521 tgcttaggtt tttcgtttta gaagaatatt gctacaaaga caggcacaac tgctacaaca
     8581 actacaccaa ctaacactaa agctatactt gccattgatt cttctacagg tcctaattct
     8641 ttggctggtg ctacacctaa tgtgtgacca cttgttccaa gtgctaatcc tcgggcaata
     8701 gggttagtaa ttcggaaaag ctttaagaat ttattaccta gggcataaat aatgacacca
     8761 tttaaaataa ctgctaatga tgttaattct tttataccac cgataccagc tgatactggt
     8821 aacgcaatcg ctgtagttgc tgcttgaggt aacattgata aaataacatc attggcaaat
     8881 tgtgctaact tcgcaaaagt taaaataatt aataacgcta caactgtacc gataccaata
     8941 cctccgatga tacgatgcca atgtttaaca agcacttcac gctttttata taacggaatc
     9001 gcaaaacaga ttgttgccgg ttctaagaag aagtaaataa tgtctccacc tattttgtaa
     9061 gtcttatacg gaatgcctgt taaatagagg aaggccacac caaataccat actgacaaat
     9121 agcggtgcga ataagaagaa acgattagtt ttttcaaata atatggtcgc taagaaaaat
     9181 ggtataacgg ataacagtat tccgaagtaa ggtgtgttta gtgctaagtg gttaatcatg
     9241 agcttgtgcc tcctctattt tgatcttttt tgtgactttg tcacctttag atctcgaagt
     9301 aactttcata ataatttgtg tgacatagcc agtacaaata agtaatagta ttgttgagac
     9361 gattattagt ccaatgatta aaaatggtgc ttggctaatg acacctaaag agttaacaac
     9421 tgagataccg gctggtacga agagtaagcc aatgttattt gttagtgtcg ttcctacttt
     9481 ttcgacttcg cctaacttaa cagcaccagt acataataat acaaataata atactaaacc
     9541 gattactgat gcaggcatag gaattggcat aaatgattca attattttcg atacaaagag
     9601 tactaaagca attacaatga cttggtgaaa aaagtgtgct ggttttgatg cgtctttttg
     9661 ttgtttcacg accattgcct cctacgtttg atttaactaa agtatagatg gctcacttcg
     9721 atttgcgtga tttttagtcc gaaatacaaa atatcatagg taaaatgcat aaaaaaaagg
     9781 attactgtta aagtaatcct atcgacgctt taaaatcttt cataaatgaa cgtccaactt
     9841 gcatcttgac accatttgtc aatattacca tataagtata gttaaaccat tgttgcactt
     9901 ctttaatgtg tttcgtgtta ataatatatg aacgatgaat acgtataaaa taagtgggat
     9961 tcaatcgttt ttcataacga ttcaatggct ctgttgtttc gtatttatga ttcgttgtat
    10021 gtatggttgt aataccatta tgtgtgccaa tcccaataat attttgttgc tttaacatgt
    10081 gaattttatc gtcaatttca acaggtaagc tttgatcaaa attcgccgac atatcattcg
    10141 caattgcact tgcgttatta tcatctttgg ctttagtcgc acgcacttta ttgactgctt
    10201 gttcaatacg tttttgacca aacggtttca aaatatagtc tgtcgcattt aattcaaatg
    10261 cctgtactgc gtattggtca tgtgcagttg caaaaataat cgcaggtggc tctttcatct
    10321 tttgaatctt agctcctaat tcgatcccat tttcatccat taaattgaca tctaaaaata
    10381 taatgtcata ttgattgatc agtagtgctt ccaatgtttc ttttacattt tctgcctcat
    10441 taatttcttc aaaaccacca atttcattta ataaatatgt taattcatta cgtgctaatg
    10501 gctcatcatc tatgattaat gctttcatat ttattcctcc tcttgtcttt cataaggaag
    10561 tacacaccaa aaagtggtac cgctcgatgt cgattcaaat tgtaatgctg cggattttcc
    10621 aaataatcct tttaggcgta agtttaaatt ttctaaagca ctaccagttc cagactctga
    10681 ttctacagat gtttctccca acaaatgcat tttatcttta gaaataccct gaccattatc
    10741 ttgtacaata atacgtacat gtgttgcagt ttctttaatc actgacacgt caatatcgtt
    10801 accttgcttt cgattcgtaa acgcatgttt gatggcattt tcaactaaaa tttgaattaa
    10861 aaatggtggt acaagcacat cgcgatattt gtcttcaaca ttaatattga tattaaatct
    10921 tcctggataa cgtgcttgtt cgagtgataa gtatgcacgc acttgactta actctttatc
    10981 taaagtaatc gtatgttgct tagagccttg taaattcgct ctgaaaaaat aactcaattc
    11041 taatagtaac tctcgtgcct tttcgctatt tattctaact aaagctgaga tcgtgttaat
    11101 tgaattgaag aaaaaatgtg gactcacttg tgcctgtaat gacttaatct cagcatcttt
    11161 caataactta ctttgcgttt cggcttcacc aagttcaatt tggctactaa aaatatttgc
    11221 caatccttct gcaagttgac gttccacaaa agttaaatca ttagggtttg taaaatacat
    11281 cttcaatgta ccgacgatag aaccatgcat ctcaagtggt atcacgatag ctgctctaag
    11341 cgggcaattc ggatgactac aaccaatctc ttctttagta tgaacttctt tcaactttcc
    11401 tgatttcaat acatctttag acagacttgt taatatttca tttgttggta tgtgatgatc
    11461 actacctgca cctacatgcg ataagatttc atttttgctt gtaattgcta cggcagatac
    11521 tttcattaaa tttttaataa tcatcgcaat ttgctgtgcc gattctctat tcaatccttc
    11581 tttaaaatac ggcaatgtct ggttcatcaa ttgcagtaca tcatgtgttt gaacagcctt
    11641 catttgctcc tcttgcttta atgttgaaat gataatagac ataaaaatcg ccgtaccaac
    11701 gctattaaca ataatcattg gtagtgcaat taatgatatg aggtcaaccg catatgcttt
    11761 gtcgtgggaa aatgttaaaa tgctcaacat ttgaatcatt tccataacaa ttccaatcat
    11821 ggcacttttc gcaatactcg ggtaacgctt gcgtctttga gcttgtaagc caaaataacc
    11881 agcaattata ccaataaata tagatgagat aagataaact tgtgcatccg ccccacccat
    11941 atacactctg aaaatacctg aaataacgcc aacaaataga cctacaaaag ggccaccaac
    12001 taatcctgcg acacctatcg ttaatacacg tgtgttagct aaagatacat catcatctaa
    12061 acggaagtac acacttcctg acaaactatg ttgatgatcg atgacgatac cagttaaatt
    12121 agacattaag gcaaacaaac tgaaaataat acataattgc caacgtgctt tccatgtacg
    12181 tcgacgattc attaagtttt taaaatatgg aatattcatc aacacatagg ccaaaataat
    12241 aattaaacct acacgctcaa gtaataacat tgttagcgat agcacgatag cacctcagtg
    12301 aattataatt atgtttattt tatcataata gtgcatgtaa aattcatgaa taaattatta
    12361 aaaaaacacg ctactattta attatctata ccattaaatt ttcatatcaa tttttatgct
    12421 aagttgacca tttttctttt caaataatgc tgtttatgtc actattatga cttttataca
    12481 tagcgaaaaa ctgctggact tatttttgcc aacagtttct aatcgcgtta tttatgatac
    12541 ttttttaaat aatacgtgat tctctaaatg tacgtgttca tgtgttaaca cttcaagtgc
    12601 ttttaatctc tgataaacaa gtcgccaagt accacacgct tctatcggag gttcataact
    12661 agatgttaat tcgctcattt ttactaacaa ttctcccgtt gcaatgtggt ctgaaactaa
    12721 atcatctatc acagtattaa tatcgtctac tacctcacct tgctcatatt taatgagttt
    12781 tggaaaatcg acatcgtctt ctttttgcat atgctctaac atgccatttt taaatgtatc
    12841 gtatgtttct tttaactcaa ctaaatatgg atgattaggt ccatgtactt tcgataactt
    12901 cgtcacataa ggtgttaaat ttttaaattc ttctcttaga ggttcatgat atgctgattg
    12961 aatatattga ataagtgatg aaacatttaa aaatttagga tttaacgaac ctggtgtatt
    13021 cgtttgttca acgtcgttga gacgctgtaa taattcgttc aaatctacat ttttcttttc
    13081 taaggctgct gcttctatac ttacttgtcc gccacaacaa aaatctattc ccacacttct
    13141 aaaaatatcc gctgctttcg gataatcagt tactacatct gctactatgt catttttatt
    13201 tatcattata gataacctcc tcatttccag tgtatacaat ttaatgtata tgaaaatgaa
    13261 aatcagggaa atgtcataat catgtcataa acagcaaaat aaagccctca aatcatgttg
    13321 gctcacaatg atttgagggc atttattatt tatttagtgg ataagtgata tgtgcagtgt
    13381 ttaacttaac aatatttttg aagtctcgct ttttaatatc tttgctaagt aagatttgca
    13441 cttcgttttc tgatgtttgg cttaacattc ccatcaccat gtcgtctttt agtacgacac
    13501 tagccatacc ttcttcaaat acataaccac gatccagacc agttaaaatg attttgtttt
    13561 taccttttaa cttcaatgag attgaatccg ttaatgtaat agctgtaaag tataacgaaa
    13621 tgttattaaa gtcttttgtg aaatacggtg ttaatctata aaaatcatta ttcaaatgaa
    13681 ttaatgcact tgtttctgca gtatatgcat atttattacg ttgattacca acacgcactt
    13741 caaatacttc cgtacctatt tggacttcta agaaaacatc ccaaattttt tctttaggta
    13801 aatacgcttt aatatcttct aacataatgt taaaacgata ttgtttattc gcattgacac
    13861 ttaaaggtac atacattgtg tgcttcttgt ttcgtgaatg taatttaagt ctgacatcat
    13921 aatgtccttc gccaatcata aattcattta acgttagctc tgtaacaatt ttatgattat
    13981 caaatttaaa tgctgtcatt ttcacttttg gcttagcgat atcaatgcct ttaaagtatg
    14041 gttcgccttc attgaattga atgtttaatt taccatctac aatatcaaaa cggtagtatt
    14101 gaccttgacg gtaactttcc tccacagttc tatagttgtc tatatctttc gctctcgcgt
    14161 agtgcaacaa tggtcgtaat ttactcatca ccaatgcatc tacacgttct ggaactgctt
    14221 gtataaagtc tcctagagca ttaatccatt gtggttgatc ttctagttta actttaagtg
    14281 agaagccatt cgtacgagaa aaactaaaat gacgatttaa aaattctgct aagatttgat
    14341 ttttatgggc ttcttctaaa tctgcattta atatttctac agcaatcaat ctcatgactt
    14401 cataaaagtc ttcaggtgaa acatacgcac tactcatatg ttcaccttca cgctttgtag
    14461 cataataata cgctttatca cttaacacac tgattcgatt tgcatttaaa tatgctttca
    14521 ttgtaaataa ttgatcttct gcactcttta attcttctgg aaaataaatg tcattatctt
    14581 ttagtaatgc tgttctatag attttagtcg ggcttaaagt atagataatt cttgaattta
    14641 acaaagtaac ttcaggtgct gtttctttaa acatagattg tggtacacca cgaccattaa
    14701 cacctttcat tttaatcaat aagacatctg agtgatgttc atcaataaat gctgctgcat
    14761 cctttaaagt ctctttgttt atatagtcat cggagtccac aaataataca aattcacctt
    14821 ctgcttgttt taacgccaca tttctaggtt tacctggacc accagaattt gtttcgagtt
    14881 gacttacttt caagttcaat ttattgcgat acttttcaac tatttgtaac gtttgatctg
    14941 ttgaacagtc atcaactaca accacttcaa attcagtttt cggaaaatct tgtttcgcaa
    15001 ggctattaag taattctgtt atatactttt ctgaattgta tgttggaact attactgaaa
    15061 atttcatcat tatacctctc ccactttgac tactatataa acttagctac caaataaatt
    15121 tctgactaaa cgctcacttg atcggccatc ttgatattta aaatgtttat ctaagaatgg
    15181 aatgactttt tctccttcat aatcttcatt gtccaaggcg tccattaatg cgtcaaatga
    15241 ttgcacaatt ttacctggaa caaatgattc atatggttca taaaaatcac gcgtcgtaat
    15301 ataatcttct aaatcaaatg catagaaaat cattggcttt ttaaatactg catattcata
    15361 tattaaagat gaatagtcac taattaataa atctgttatg aacagtatat cattaacttc
    15421 tctaaagtca gaaacgtcaa caaaatattg tttatgtttg tctgcaatat taagtctatt
    15481 tttcacaaat ggatgcattt taaataatac aaccgcgtta tttttttcgc aatatcttgc
    15541 taaacgttca aaatcaattt tgaaaaatgg gtaatgtgct gtaccatgac cactacctct
    15601 aaatgttggt gcgaaaagaa tgactttctt acctttaata attggtaatt catcttccat
    15661 ctcttgtttg atctgtgtcg cataagcttc atcaaatagt acatcagtac gtggaacacc
    15721 tgtaggcact acatttttct ctttaatacc aaatgcttca gcgtagaatg gaatatcggt
    15781 ttcagatgat acataagctt ttgtatagct acgatgattt aatgaatcaa taaatggtcc
    15841 acccttttta ccagtacgac taaagccaac tgttttaaag gcaccaacgg catgccatac
    15901 ttgaataact tcttgagaac gtctaaaacg cactgtataa atcaatgggt gaaagtcatc
    15961 aacaaagatg tagtctgcct tcccaagtaa atatggcaat ctaaacttgt cgatgatgcc
    16021 acgtctatct gtaatattcg ctttaaaaac agtgtgaata tcatactttt tatctaaatt
    16081 ttgacgtaac atttcgttat agatgtattc aaagtttcca gacatcgttg gtctagagtc
    16141 tgatgtgaac aacaccgtat tccctttttt caagtggaaa aatttcgtcg tattaaatat
    16201 cgctttaaaa ataaattgtc ttgtattaaa tgattgtttg cggaaatact tacgtaattc
    16261 tttatattta cgaacgatat aaatactttt aacttccgga gtcgttacaa caacatcaag
    16321 gacaaattca ttaacatcgc tagaaatttc aggtgtaaca gtataaaccg ttttcttcga
    16381 aatgccgcct tttctaaatt cttttaggta agtctgcaat aagaaattga ttttaccatt
    16441 ttgtgtttct aattcgttgt attcttcttc ttgttctggc tttagatttt gatatgcatc
    16501 attaatcaca tctgggttta actgtgcaat ataatcaagt tcttgctcat tcactaataa
    16561 gtacttatct tcaggtaagt aataaccatt atctaagata gctacattga aacgacaaac
    16621 gaattgattc ccatctattt tgacatcatt cgccttcatt gtacgtgtct cagttaaatt
    16681 tcttaataca aaattactat cttctaaatc taggttttca ctatgtcctt caacgaataa
    16741 ctgaacacgt tcccaataga ttttatctat atatatctta cttttaacca acgttaattc
    16801 atccttttct atttacataa tccattttaa tactgtttta ccccaagatg tagacaggtc
    16861 tgcttcaaaa gcttctgtaa gatcattaat tgttgcaatt tcaaattctt gaccttttaa
    16921 caacgctaat ttatctacaa tatctgggta ttgaatgtat aagtctacaa catcttggaa
    16981 atcttttgaa ccacttcgac tactaccaat caacgttagt cctttttcca atactagacg
    17041 tgtattaact tctactggga actcacttac acctaacagt gcaatgcttc cttctggtga
    17101 aatgtaatcg atcatttgat ttatggctga ttgactaccg cgaccaccca cacactcaaa
    17161 tgcatgatca aatgttaagc cttcaggtat tttattaata aagaagacat catcaacaaa
    17221 tgagaagtga ctcaatttat aatctgtttt accaaagaca tatattttag actctggata
    17281 taatttacgt aataaaatgg ctgtaatgta acctaagtta ccatcacccc aaataccaaa
    17341 tgtattttta tttgaaatag atttcttttc aaaacgacga atagcatgca aacttactgt
    17401 tacaagctct gtatatgaaa taatacttaa atcaatatca tcaggtagtg gtacagctct
    17461 atcatgattt agcaacacaa aatcttgcat aaacccatca tgtccacttg atctgaagta
    17521 gctcgatttt aaatagtttt cagcaatgac atcgtctttt tctgtcggcg tattcggtac
    17581 cataactact tttgtacctt tattaaacac acctttactg tcaaatacga cctcaccaac
    17641 accttcatga attaaagaca ttggcaattt ctgagataag acattctcat cacggctacc
    17701 agtataatat ctttgatcgg cagcacaaat tgacatatat aaaggacgta caatgacata
    17761 gtcactgtaa atatctacgt tgttatacgt aacttcaaat tgtctaggtg caactaattg
    17821 atatacttga ttaatcatcg gcaataccac ctcgaataat agcattcgct acttttaaat
    17881 cgtaaggtgt tgttacttta atgttatata actcaccttt tacaagtcga accggtttgt
    17941 ttgtttctac aataatctta caagcatcag ataaaatact cttttgctca tcactcaact
    18001 gtgcatagct ttcttttaat aaattaatat taaacgattg aggtgtttga ccttggtaca
    18061 tttcattacg cactggaatt gcatcaatcg tttgattatc tttagatgta acaatcgtat
    18121 ctatagcatc aatcactgta tctactgcac cgtactctaa agcagcttga atattttctt
    18181 taataatacg atgcgttaaa aatggtctaa ctgcatcatg tgtcacaatg acatcgtcat
    18241 cgttaatacc atttgttgat tcaatatgtt taacgatatt cataattgta tcgttacgat
    18301 cgctaccacc ttgaatgact tcaattcttt catcagaaat tttgaatttt ctaagtgtat
    18361 ctttcgtatg cgtcatccat tgttgtggcg tcgcgataat aattttttca aaatcattaa
    18421 ttaaaataaa tttttctaat gtatggatta aaatcggttt gttgtctaaa tctaaaaatt
    18481 gtttaggtaa aggtacgtta cccattcttg agcctatacc tccagctaga ataccagcgt
    18541 atttcataaa atacttcctc cattcaacta tatctatatt taattattta aatttcgttg
    18601 cattttccaa ttgaaaactc attttaaaat caaaactcta aatgtctgtg tattacttaa
    18661 aattatacat attttgctta tattttagca tattttgttt aaacctatat tacattatat
    18721 cagacgtttt catacacaaa taataacata caagcaaaca tttcgtttat tatttatatc
    18781 acttaactaa ttaatttata attttttatt gtttttaagt tatcacttaa aaatcgtttg
    18841 gcaaattcgt tgtgacgctt gtccatcttc taatgaacag aatttttgat aaaataccgt
    18901 tcgtgcttca atatactcat ttgcagtctc atcgatttgt tttaatgcat caatgagtgc
    18961 tgtttgattt tcaacaattg gacctggcaa ctctttttta taatccatgt aaaaacctct
    19021 aagctcatcg ccatatttat ctaagtcata tgcatagaaa atttgcggac gctttaatac
    19081 accgaagtcg aacatgacag atgagtagtc ggtaactaac gcatcgctga ttaagtataa
    19141 atccgaaatg tcttcataat ctgaaacgtc tttcacaaaa tcatcatgtt catcaatacg
    19201 tgtcacaact aaataatgca tgcgtaataa aataacataa tcatcatcca gcgcttgacg
    19261 caaagcttct atatcaaagt taacattaaa ttgatatgaa ccttctcgaa tcgcttcatc
    19321 gtcacgccaa gttggcgcgt acataatcac ttttttatct aatggaatat ttaatcttgt
    19381 cttaatacca ttaatatatt cagtatcatt gcgtttatgt gataatttat catttcttgg
    19441 ataacctgtt tccaaaatct tatctcgact aacatgaaat gcattttgaa atatcgatgt
    19501 cgaatatgga ttaggtgaca ctagataatc ccaccgttgg ctttcttttt taaagccatc
    19561 ttggtaattt tgagtatttg ttcctagcat tttaacgtta ctaatatcca aaccaatctt
    19621 ttttaatggc gtgccatgcc atgtttgtaa gtacgtcgtt cgcggtgatt tatataacca
    19681 atctggtgta cgtgtgttaa tcatccacgc tttcgctctt ggcatcgcta aaaaccattt
    19741 cattgaaaac tttgtaacat atggtacatt gtgctgttgg aatatgtgtt catatccttt
    19801 tttcacaccc catattaatt gggcatcgct atgttcagtt aagtattcat ataatgcttt
    19861 ggggttgtcg ctgtattgtt taccatgaaa gctttcaaaa taaattagat tcttgtttgg
    19921 caattttgat agtaatttaa aagtcgtata tatactatgt tctatcaatt ttttaattgt
    19981 atttttaatc atgtcgtacc tccgacgtgt ttttgtaatt atattaatat gtatgagcaa
    20041 gctcattgta accatgccta ttatagcatt tcatcataaa atacatttaa ccattacact
    20101 tgtcgttaat tatcatacga aatacatgat taatgtacca ctttaacata acaaaaaatc
    20161 gttatccatt cataacgtat gtgtttacac atttatgaat tagataacga ttggatcgat
    20221 tattttattt tacaaaatga caattcagtt ggaaggtgat tgcttttgat tgaatcgcct
    20281 tatgcatgaa aaatcaaaag gttattctca ttgtatagtc ctgcttctca tcatgacatg
    20341 ttgctcactt cattgtcaga acccttcttg aaaactatgc cttatgactc atttgcatgg
    20401 caagtaatat atgccaacat tagcgtctaa acaaatcttt gactaaacgt tcacttgagc
    20461 gaccatcttg atatttaaaa tgtttatcta agaatggcac aactttttca acctcataat
    20521 cttcattgtc caaagcatcc attaatgcat caaaggactg tacaatttta cctggaacaa
    20581 atgattcaaa tggttcatag aaatcacgcg tcgtaatgta atcttctaag tcaaatgcat
    20641 agaaaatcat cggcttttta aatactgcat attcatatat taaagatgaa taatcactaa
    20701 tcaacaagtc tgtaacaaag agaatatcgt taacttcacg atgatctgac acatcgataa
    20761 agtattgtct atgttcacgt gaaatattaa gtctattttt tacgaacgga tgcattttga
    20821 ataacacaac tgcattatgc ttctcgcagt atcttgctaa acgttcaaaa tcaattttaa
    20881 aaaatggata atgtgccgta ccgtgaccat tacctctaaa tgtcggtgcg aatagaataa
    20941 ctttcttacc ttttataatt ggcaattcat cttccatctc ttgtttaatt tgtgttgcat
    21001 aagcttcatc aaatagtaca tcagtacgtg gtacacctgt tggtacaaca ttttcttctc
    21061 taattccaaa tgcttcagca taaaatggaa tatcggtttc tgatgaaaca tatgctttcg
    21121 tgtaactacg atggtttaat gaatcgataa acggaccacc ttttttacct gtacgactaa
    21181 agccaactgt tttaaaggca ccaacagcat gccacacttg aataatttct tgtgatggtc
    21241 taaagcgaac cgtataaatt aatggatgga aatcatcaac aaaaatataa tcggccttac
    21301 caagtaaata tggcaatcta aacttgtctc tccatttgcg tctatccgta atattctcct
    21361 taaataccgt tttaatatca taatcaaaat ctactttttg gcgtagtaac tcatcatata
    21421 catacttgaa attccctgat aaattcggac gcgaatctga tgtgaataat attgttttgc
    21481 ttcttttaat atgtagtaac tttgtaatat taaaaatagc tttaaataag aaacttctac
    21541 tttcaaatga agctttatga ccttgtttat gaagccagtg tgcacttgtc gcaatgaccc
    21601 ctgatttctc ttgaggtaag gtgatttcaa tatcaaatac aaattcgtta acgtcacttg
    21661 ctatttccgg agtaatggta tagaccgtat gttgtgatac gccaccttta cggaaaactt
    21721 tagcatcata cgctaataaa tagttcactt ttccattttg tgtagtcagc tcattgtatt
    21781 cttcaatttg ctcttcattt aatgccgcat aagcttgatc aataatattt ggattaagtt
    21841 gtccaatata ctctaactgg tcatgataaa caaataaata tttatccatt ggtaaataat
    21901 acccatcgtc taatattgcg acgttgaatc tacatataaa tgtattaccg tctattttca
    21961 tttcatttgc ttctaatgtt tttgtttcgg ttaaattcct cagaacaaat tgtcctgatg
    22021 ttaaatcgac accttcacta tgtccttcaa tatataactg aacacgttcc cagtatatgt
    22081 tatcaatatg tattgcttgt ttcgtttttg tcattgttat tcccccatcg gtttattaaa
    22141 tcgtccattt caatactgtt tttccccaag atgtcgataa atccatttca aacgcttgga
    22201 cgatatcttg catcgtacat acattaattt catgtccttt taataatgct aacttttcaa
    22261 ctatgtctgg gtacttacga tataaatcaa caacttgctc aaaatcttta gagccgcttc
    22321 gactactacc aatcaacgtt aatccttttt caagtactaa tcgtgtattc acttccacgg
    22381 gtaattcact tacgcctaac aaagcaatac tgccttctgg tgaaatatgt tcaactattt
    22441 gttgaagtgc aacttgactt cctttacctc caacacattc aaatgcatga tcaattttaa
    22501 gatcatctgg tatttgattt actgtaaaga tgtcatctac aaatgaaaaa tgacttaatt
    22561 tatagtctgt cttaccaaat acataagttt tagcttctgg gtacaactta cgtagcaaaa
    22621 tagcagtaat ataacctaag ttaccatcac cccaaatacc aaagctggtt ttcaaaggta
    22681 tagatttacg ttcaaatcgt tgtatagcat gataacttac tgacactaac tctgtgtatg
    22741 aaatcgtact caaatcaatg tcattaggca gcggaacgat acgatcatgt gccatcacaa
    22801 cgtagtcttg cataaaacca tcataaccac tagatctaaa ataactagag gctaagtaat
    22861 tctccgcaat aatatgatgt tgctctgtag gtgtattcgg taccattact actttcgtac
    22921 ctttttcaaa taccccttta ctatcaaata caacttcacc aacagcttca tgaactaatg
    22981 acattggtaa ttttttgcgt agtacatttt catctcttcg acctgtgtaa tacctttgat
    23041 cagctgcaca aatagacaag tataaaggtc ttacgatgac atgattacca taaatatcaa
    23101 cattattata tgtgacgtcg aactgtctcg gtgcaacgag ttgatatact tgattaatca
    23161 tcggcaatat caccttgaat aatggcattt gctactttta aatcatacgg tgttgtcact
    23221 ttaatgttgt atagttctcc acgtaccaat ttaactgcat gtccagattc gacaatgatt
    23281 ttacatgcat ctgataagat ttctttttgt tcactactta aggcgcgata actatcttgt
    23341 aataatttaa tattaaatga ttgtggtgtt tggccttgat acatttcatt ccttacaggg
    23401 atactgtgta tgttctgttt atctttagac attacaatcg tatcaattgc ttcaatgact
    23461 gtatctactg caccatattt tgctgctact tcaatgttct ctttaataat acgttgagtt
    23521 aaaaatggtc ttacggcatc atgagttaca atcacatcat cattattaat tccatttaca
    23581 ttgcgaatat ggtcgataat gttcataatt gtttcatttc gatccgtacc acctgcaact
    23641 actttgacac gttgatctgt aatgttatat ttttttaaaa tatcctgtgt atgggaaatc
    23701 cactgtgctg gcgttgcgat aataatctca ttaaattcac tcactaaaat gaacttctca
    23761 attgtatgga ttaaaatcgg tttattatca atatctaaaa attgttttgg taatggcacg
    23821 ttccccattc tcgaaccaat acctcctgct aaaatacctg cataaatcat gttgtcctcc
    23881 attctgtcat tacatcattt ccatttatac attactgacc tatgcccgca cataagccta
    23941 acctattgct cacttgcctc ttttattaat ccaaagatag ttgtcacaat agtgtgataa
    24001 ttttttataa aaatgtattt ttgtaactga ccattctaag ttgttttgcc atgcagttaa
    24061 tcattaactc tgacgatatt aaattgttaa aggtattaat gtttactctt tttcaaattc
    24121 attattactg ccatcatttt accatatatt ataataaatt tatcttatta agtggctgta
    24181 cttgattttc actttaaaaa ttatcaaata ttgccatctc attttaagta tacaaaatgc
    24241 aaaacaaccg attcacaagc atatttcaca caagtaaacc ggctatttat caacgtatat
    24301 tcgaagatga attatttcga tagtatctat agaccagacg gcattcgcac tttcatagct
    24361 ataactatac cagcgttttc gtcctcaaag gtgcatacta ataaatcgta aacatgactt
    24421 tatcaaatcg ttctttcttg ttaactaatt tatcaaatgt ctccgggcct ttttctaacg
    24481 gtaaaaaatg agaaataata ggctttacat taatatcttt cgtcttcata taatgtaagg
    24541 ttgccgtcca ctctttgccc ggaaaattac tggacaaaca gttccaagag ccacatactg
    24601 tcaactcgtt acgcagaatt ttttcaaaat gaacgcgatc aatctcaata tcatcatatg
    24661 gtattccgag taataccacc tcgccacctt ttttaggtag cgtcaatatt tgaccaatcg
    24721 taactttagc acctgatgat tctatagcta aatcgatttg attggcgtaa tgattttcga
    24781 tgaatttctc aagattttct tcttttgaat tgattgtttg atgtgcgccc aatgatgttg
    24841 caatatctag tttatgcgca tctatatcta tagcgatgat atgtgcagca ccaaatattc
    24901 gtgcccattg aatagctaac aaacctatac tgccacaccc cattactgca acagtcatac
    24961 caggttgtat attcgattta taaaacccat gcgcaacaac ggctgatggc tcaaccattg
    25021 ctgcttcaat gtaatcaaca ttgtctggaa cctttaaaac attttgcgct ggcaatttga
    25081 catattccgc gaacgatcca ggttcatatg agccaatgac gaataacttt tcacatcgtg
    25141 catattcacc ttttaaacaa tactcgcatt gataacaagg tattgctggg caacctgtca
    25201 ctttgtcgcc cacattaaca tgcgtaacat cacttccaat ggcatctact acacctgaaa
    25261 attcatgacc aaatggcata cctttaatgt atggccccat ttttttgtat cgtgacgtgt
    25321 ctgaaccaca tatgccagtc gctcgtactt taataataac gtcattcgca ctttcaatga
    25381 ctggcttttc attatcctca taccgtaaat cttccacgcc atataatttc aatgctttca
    25441 cttgtaaatc acctcaaatt tgatttaatt cacaactttt ttctttttaa aaatacctgt
    25501 cgcaaaataa cctgcaatga caatggaatt acttacgagt aaatgttcca tataaaaatc
    25561 agtgatttgt cttaatggcc caagcataaa agttagcatt gattctacca tattaattct
    25621 ctccttcatt ttcatctaaa tcgagcggct taagtaatac tttaatacct tcgccggatt
    25681 ttaaatgttg atacgcctcg tcccatttcg aaatatcata tatttttgtc accaaagctt
    25741 cagcatttac taaaccatcc gccataagtt gcaatgaagg ttcccaatct gctggctttt
    25801 gacttctact accaacaact gttatttctt tttgaatcac tttttccata tcaaatggaa
    25861 tttcagcatc cttaaaaata cctatttgac tgtagaaacc ttttttgcgt aaaatatcca
    25921 aaccttgtcg tgctgctgga actgcacctg aacattcaac aacaacatct gcaccgtaac
    25981 cgtctgtaat tccattgata tacgttttta agtctgtttg ttgtaaattg actacataat
    26041 ccatgtgcaa tgcttctgct ttatctaatc tgactttgtc attgtccaat ccagttacca
    26101 caacagttgc gcctttactt tttaacactt gtgctacaag taatccgatt ggcccaggtc
    26161 ccattacaac tgctacatcg cctgaattga cttgaatctt agaaacgcca tgatatgcac
    26221 atgctaatgg ttctgtcata gctgcagact gatacgatac ttcgtctgga atatgatgca
    26281 aactttcttc acgtgcaatg acataattag taaatgcgcc atcaacttgt gttccaatac
    26341 cttttcgatg gttgcataaa ttatagtctt ttgatttaca gtattcacac tcattacaaa
    26401 catagaatgt cgtttcagat gtgacacggt caccaacttt aaaatcttta acgtctgctc
    26461 caacttcaac gatttcacca gaaaattcat gacctaatgt cactggaaaa ttaactttat
    26521 aatgaccttc ataagtatga atatctgtgc cacaaattcc tgcataatgt actttaatct
    26581 ttactttatc atctagcggt gttgcaactt ctttatcaag aagttctaag ttgccatgtc
    26641 cttctcttgt ttttactaaa gctttcacca caaacacctc gatttttaat tgaatagact
    26701 aaatagttta aagataagat agttaacgat attaccacct tgatcaatac ttgaaatttc
    26761 agatgaacct tttggcattt gtacattcgt acctttcgcc atatctgtga aaatgggtgc
    26821 tacgtctgtt gcaatatata gtgaaattgc aatcataatc gtacccacaa tgacagaatg
    26881 aataatgttt cctcttgctg caccaacaat aaacgcgaca acaaatggta tcgttgctaa
    26941 gtcaccaaaa ggtagtactt ggtttcctgg taaaataacg gctaataaaa cagtgatagg
    27001 tactaaaatt aatgctgtcg aaataactgc tggatgacct aatgctacag ccgcatccaa
    27061 tccaatataa atttcacgtt cgccaaaacg tttatttagc catgttcttg cagactctga
    27121 aactggcatt aaaccttcca ttaagatttt taccattcta ggcattaata ccattactgc
    27181 agccattgac attcctaaat taatgatgtc tccaggtttg taacctgcta acacaccaat
    27241 acctaaacct aaaatcaagc cgacaaatat agactctcca aatgcgccaa aacgtttttg
    27301 aattgtttca ggatcagcat ctaacttatt cagaccgggt actttttgta acaatttaac
    27361 taagtaaata cctggtgcat aagaaattgt acttcctgta gcaatcgata cacctggtaa
    27421 atcaaagaac tcactcatca tcggtgctgt ccaatctgct actttcaaac agataatttg
    27481 gaaaataact gctgctaata acgcttgcca aatactgcct gatacggcat aaaccattgc
    27541 tgctgtaaac gtataatgcc aaaaattcca aatatctaca ttcatcgtct ttgtcacttt
    27601 agttactagc aatacaacgt taactatgat tccgagtgga ataataaatg ctgcgacaga
    27661 tgatgcccaa gcgatagatg atgttgctgg ccaacctaca tcaatcacat tcagactgac
    27721 gcctaaattt ttaaccatcg cttgtgctgc tggccctaaa tttttaacta ataaatcgat
    27781 gactaagaaa atccctacaa aagccacacc tattgttaaa ccagacctaa atgccgctcc
    27841 aattttctgc ctaaagaata ggccaagcaa gaatatgaca accggtaaaa taacagttgc
    27901 acctaaatct aaaaatcccc ttacaaaatc agtgaagtaa ctcatattta aaccctccct
    27961 gttatatatg cattgtcacg atactttccg attgtgatta catttgacgt tacagtcatt
    28021 tcaacgacaa cccttgctaa attcgactgc agtccttttg aattacagtc actgcgtttc
    28081 tatgtcatca acaatcattt gtcgtgatag tcatttatat gcaatttgca tatattaata
    28141 tgttatcgac ccacgttaca tatcaattcc gttatttttg taactctgtt aagatttgtt
    28201 gttttgtttc ttcaatacca ataccagtta agaaattacg tgcgttgata actgggaatt
    28261 tatattcttt ttttgtcatt gcagttgtaa ctaataaatc tgcagtgtct tcataaggtc
    28321 caacttctgt aattttgatt tgtttaatat ctactttaat attgtgttcc tttgccattt
    28381 cttcaattgc attatttact actgttgacg ttgcaatacc tgcaccacac gctactaata
    28441 cttgtttcat tttcaattcc tccaattaat ttttagttat attccaaata atcattgatt
    28501 agtgttgcta aaattgtttc atctttcgtt cgtagaatct gctccaattt ttcttcactt
    28561 tgaaaaattt gcatcaactg ttgtaacagc ttaagttgat catctacttt atccattgct
    28621 aacataaaaa cgattttcac ttctgtctgt tgatcaagtg ttcccatttc aataaacggc
    28681 acttcttttt ctagaacagc cacacctatc gttctatggt taatatgttc gacatctgta
    28741 tgcggtatag cgaccgaaca tagatgcgtt ggtaaaccag tagcaaattc tttttctctg
    28801 tcgatgactg catctttaaa cgttgacttc acgaacccat tttgaaataa cacatctgac
    28861 atttgtgaca atacggattc tttatcagtt gccgacaaat tgagcattat attttcttta
    28921 tgcactaatt gctgtcccat ccattttccc tcgcttcttt atttgaataa ttttttaaaa
    28981 tctcatttac atcagaattt ttgcgacttt gtatgatgcg cttaattgcg tcattgtctt
    29041 gcgccacatc tctcaattgt agtaacgctc ttaagtgtgt cactttatca acagcagcaa
    29101 taggtacaat aatatggatt gctgtgccat ctgacatgta tattggttct tgtaatatca
    29161 acatactcat cgctgtttta tgtacatgct tttcagagta tgcatgtgga atagcaatat
    29221 tttgcatgat aaccatatac gaatcatcaa atacctgttg catctcatga atatatttaa
    29281 tatcaataaa atgattagca actaacacat cacttgcttt agcaatagct tcatcaatat
    29341 tttcaacatg atgcattctt ttcacgtgcc ttgccggtat caagtcagct aaatctaatg
    29401 tcttattttg tgtgacaatc gatccattaa tggttgaaat tgaattataa ttggcaataa
    29461 aatcttctaa accatcacgt agttctgtaa tgtcattaac tgtcgttgtg cgttcaatta
    29521 atgccattaa cttgtttatt tccttatcaa tgtcagccga ttccttatta atgtacttca
    29581 tcacttcttt acgtaacttt cgttgctcat tttcagataa agctactttt gtgataaata
    29641 attttttatg tgttaggaca aacattggtg aaaagacgat gtcataatct aatgtgtaat
    29701 tttcaaatgt tctaagtgaa atcgcatcta agaaaataat ttctggaaat aagtttcgca
    29761 actcgtataa catcatttgt gatactgacg tgccttgtgt acacacgata atagctttta
    29821 tcttgccatc gaagttttca tcttgacgtc tcaaactacc tccgaacaac atggttaaat
    29881 atgctatttc attatcaggc aacgattttc cgaaatattc agttaacgat tgacatgatt
    29941 gtttcaccat atgaaataag gattgataat ttccttgtaa aggatttatt aattcatcac
    30001 gatccgttaa gttatattta atcctataaa aagcaggcgt taaatgtaac aagagttgct
    30061 gtgataattt ctccttatct tcaatgttaa taaaagtgat ttgttcaaaa tggtgaatca
    30121 tttgagcgat ggccatcgtt aaattcgata tgctatctga ttcttgcaaa tcagtccatt
    30181 gcacacttgt tgaaagtaag tgtaatgtca aatataactt ttccgcttct ggcaaatccg
    30241 gctcatgttg cgtcataatc tccgttgctt gatattcttt cgtatccctc aaatactgat
    30301 aattaatatt taatggattc atcacatgac cactttgaat tcgtctacga atcacacaaa
    30361 ggacataagg caatgaacta agtgatttgt ctataaagcg actcttcaaa aattgttcta
    30421 cctgtttgat cttgtctttt tgatatgcga tatcttcgaa tgttaagttg agcgccttta
    30481 aaacttcact tttagtaata tcatgattca acctttgatc aatcaactta atgaagaaac
    30541 ggcgaacttc aaattcatca ccaacaattt cataaccatg ttttcgagaa tacttaagtg
    30601 acaaaccatg attttccaat tgctctttca catgatttat atcgtgaatg acagtatttt
    30661 tactgacttg taaatcaatt gaaaaatggt ttagagacat tgcgttttcc ttactaaaaa
    30721 gcatgagcat taaataataa cgacgtgttt ctatgctaaa aatgacattg ttgccgttta
    30781 acatttgctg ctccgataca tctcgcttga ataacgtcat gatttcagaa cttacaataa
    30841 aatttccttg gcttgttctt tcaagttttg gataaccctc ttgttcaagc cacaaattga
    30901 ttttttgaat gcgatatcct agttgtctac gagacaaacc aaatatcgat tcaagttctt
    30961 taccatgaat agtaggattc aatacaattt ctctgagtat ttgctgatta tccccgttca
    31021 tgttcgattc tcctttgagc atctgagctc aatttatcat caatcgtcac accgatgtac
    31081 acactttgaa cacatatttt caaaatgagc atgtacatca ttgtgatgtt ttaacaacat
    31141 ttcaattata tctatatttt ttgtgatttt aatcttttaa aataaagcaa ttgaaatttt
    31201 tgcatatatt tttgtgtttt gtgttttttt gaagcatttt taacatacat atctcaatca
    31261 ttatcaaatt gtcatgacca ttgtaaccca atacaaaaac cctaaggacg cttatatcag
    31321 gcgccttagg gttaactgta tctatttaat taagtattat tattcgtatg tacgtaactt
    31381 atggtctatc aagttccaca cttcttcaac atcaactgct gtagcaaaat aagcattggc
    31441 aggcttacct gtaacatgat ttaaatcgac agccatagtg ccataagtta gtggactttg
    31501 atgttcaatg tcgatattaa cgggtaccat tgtaaacaat tctggttgta acaaatacaa
    31561 aattgtacaa gcatcatgta ttggaccacc atccatatta aagtgagtct tgtatgtctt
    31621 cttaaagaat tgcaataatt ctacgacgaa ctgtgcaaca ggattattga tactttcaaa
    31681 gcgttcaatc acgtgatcgt cggctaaaac ttgatgtgtt acatctaaac caaacacatt
    31741 tatagtaatc ccactttcaa aaacacgctt cgctgcttca gcatctaccc aaatattgaa
    31801 ttctgctgta ggcgtccaat ttccaaatgt accaccaccc atcaaagtaa tagattcaat
    31861 atgctcagcg attcttggct cacgaatcaa tgccgttgct acattcgtaa gaggacctgt
    31921 cgctacaatt gttacaggtg tatcactcgt catcactttg tttataatca catctgatgc
    31981 tggcattgca actgcttgac gtgatggtgt cgacggtagt ttcggaccat ctaatccaga
    32041 ttccccatgt atttcagaag caaaggcagc tggtttaatt aacggcctat ccgcaccttt
    32101 cgctactgct atatcttggc gtcccataat atccaatacg ttcaaggcgt ttgtcgtatt
    32161 cttgtcaact gattgattac ctgcgactgt tgttacagct aatatctcta gtggactgtc
    32221 aattgccccc gctaaaatta atgctattgc atcatcgtgt cctggatcac aatccataat
    32281 aatctttctt ttcatttata tatccacctt tcttaagttg ttatcgatag cttatgtata
    32341 tttatttatg tggtgaatca tgtttatttt gaaaaatagt tttaactttc tcatattttt
    32401 ggatacaaac actatttatc tattttatgg cttataaatt tatccgatat gccttatcaa
    32461 cctacctcgc taaaaatagg atgtctacat atctataccg acttttgtca actcattttc
    32521 acaacaatat aaacagcaat ttatatgatt gttacatgat tcaaacaatt tttatgaaaa
    32581 atattttcat acacagaata tatattgata ttaaatttct caaaagctat attgagaata
    32641 attaggaggg atgttgatga aatctttatt tgaaaaagca cagcagttcg gcaagtcctt
    32701 tatgttacct atcgcaatct taccagctgc aggtctattg ttgggtatcg gtggtgcatt
    32761 aagtaatcca aacaccgtta aagcataccc tattttagat attaccttat tacaaaatat
    32821 ttttacatta atgtcagctg caggtagtat tgttttccaa aatttaccgg tcatctttgc
    32881 aattggtgtc gcaatcggat tatctagaag cgataaaggt actgcaggtt tagctgcgct
    32941 gctcggtttc ttaattatga acgcaactat gaatggctta ttaactatca cgggcacatt
    33001 ggcaaaagat cagcttgcac aaaatggaca aggcatggtg ctcggtatac aaacggttga
    33061 aaccggtgtt tttggcggga ttatcacagg tattatgacc gcaatacttc acaacaaata
    33121 tcacaaagtg gtattaccac cgtatttagg tttctttggt ggctctagat ttgtccctat
    33181 tgtcacagca tttgccgcaa tctttttagg tgtattgatg tttttcattt ggccaagcat
    33241 acaagccggc atttatcatg ttggtggatt tgtaacgaaa acaggtgcca tcggtacttt
    33301 tgtttatggc ttcatcttaa gattgttagg tccactcggt ttacaccata ttttttactt
    33361 accgttttgg cagacggcac ttggtggtac tttagaagtc aaagggcact tagttcaagg
    33421 tacgcagaac atcttctttg ctcaacttgg tgatccagat gtgacgaagt attattcagg
    33481 tgtgtcacgc tttatgtcag gccgttttat tacgatgatg ttcggcttat gtggtgccgc
    33541 acttgcaatt tatcacacag ctaaacctga acataaaaaa gttgtcggcg gtttaatgtt
    33601 atccgctgca ctcacttcat ttttaacagg tattaccgaa cctttagagt ttagtttctt
    33661 gtttgtcgca cctattcttt atgtaatcca tgccttcttt gatggattag catttatgat
    33721 ggcagacatt ttcaacatta caattggtca aaccttcagt ggaggcttta tcgatttctt
    33781 actctttggt gtgctacaag gtaatagtaa aacaaactac ctatacgtca tacctattgg
    33841 aattgtgtgg ttctgtttgt attacatcgt tttcagattc ttaattacga aatttaattt
    33901 caaaacacct ggtcgagaag ataaagctgc agcacaacaa gttgaggcta ctgaaagagc
    33961 acaaactatt gttgctggtt tgggaggcaa agataacatt gaaatcgttg actgttgtgc
    34021 aacgagacta cgcgtcacac ttcatcaaaa tgacaaagtc gataaagtat tactcgaaag
    34081 tactggtgcc aaaggtgtaa tccagcaagg cactggtgtg caagtaattt atgggcctca
    34141 cgttacagtt atcaaaaatg aaattgaaga attgctcggg gattaagact aaccgaaata
    34201 tcaacagaac taatggcaac gatgtacgaa gtaagaagtg acatcgttgc ttttattttt
    34261 aatgttacat ttgaagcatt aagttcatca tgcactgtag tgagcccgca aatcgcctct
    34321 gctagacaat catcttaatg ctatgattaa agcttaagtg ccagatttga atttaatttc
    34381 aacaacgact ttcactacat taaaaatagg gctactcgac acatatagtt gtatcaaata
    34441 gccctttata caattttttg ggtaaggttt tacaattttt gggatggtat agattttata
    34501 aaaagttatt taagttcttc tgcttcagcc ataatatctt ttaatgtttt agctgaatgt
    34561 gcgaacttgc tttgttcttc gtcgtttaat gggatttcta cgacgttgcg aataccgttt
    34621 ctattgatga ctgctggaac accaatataa acatcttctt cctcatattc gccttctaat
    34681 aatgctgata cagtcaatac ggcatcttca tttctgaaaa tcgcttcagt aattctagct
    34741 aatcccattg caacaccata ataagtggca cctttagctt gaataatgtc atatgctgca
    34801 tcacgtgttt gaacaaaaat ttgttcaatt tgcgctttgc cctcaggacg ttgttcaagt
    34861 aatgtcttca aaggttgacc cgcaatatta gcgtgtgacc atactggtaa ttcagtgtca
    34921 ccatgttcac caataatttg agcatcgacg ctacgtggcg caacatcgaa cgcttcgctt
    34981 aacaataatc taaagcgtgc agagtctaaa attgtaccag aacctataac acgttcttta
    35041 ggtaaaccag agaatttcca tgttgcatac gctaaaatat caacaggatt tgtagctacc
    35101 aagaaaatac catcaaattt tgatgccatt acttcaccaa caattgattt gaatattttc
    35161 aagtttttag atactaaatc taaacgtgtt tctccaggtt tttgtgcagc accagcacag
    35221 atgacaacta gatccgcatc atgacaatca ctgtattcgc cagctttcac acgaactgtt
    35281 gttggagaat atggtgtggc atgttttaaa tccataacat ctcctcgaac tttttcagtg
    35341 tctaaatcaa tgatgactaa ttcatcaaca atgctttggt tcactaatga aaatgcgtag
    35401 cttgaaccta ctgcaccatt acctattaat acaactttgt tccctttaaa tttgttcatt
    35461 acaaaaactc ccttatgatt aattcactaa catacatgta gcttcaaata tgttagttta
    35521 atgctgctta ttgacgatac aaaagcaaat aaacatctct tttattttca acgcataact
    35581 taaaaggtca tgtgtcatcc gcttttaagt ttgtgattta tttcacatat aaaatgtaac
    35641 atgcattaag tactgggtca atattaaatt gtgatttatt tcacatttta ttttaatttt
    35701 tacacctttt taatttgtat acgattacat cttagatgtc tttagtcttc gtacttcgcc
    35761 agtgattatt tacactttca catttttatt atcatgttta cttttttcta ggaaaacaac
    35821 aatgtttttt gaattagtca aataaatgcg ctcaatcgtc ggtgtgcaaa cagacaattg
    35881 tacacaatgc ttattgataa gtatttaaaa aattaaaaat gtcatacaat tatcaaattt
    35941 gccattttat ttatattttc tcaaaccaat taattgaata tcgaaatttt tagtagaata
    36001 atcaaaatat acagattaaa ggaggagtat catgcttaca gaacaagaga aagacattat
    36061 caaacaaacg gtgcctttac ttaaagagaa agggacagaa attacgtcaa tcttttatcc
    36121 aaaaatgttt aaagcgcatc ctgaactttt aaacatgttt aatcaaacga accaaaaacg
    36181 aggcatgcaa tcttcagcat tagcacaagc tgtaatggcc gcagcggtta atatcgataa
    36241 cttaagtgtt attaaaccag tcattatgcc agtcgcatat aaacactgcg cactacaagt
    36301 ttatgctgaa cattatccaa ttgtggggaa aaatttatta aaagccattc aagacgtgac
    36361 aggattagaa gaaaatgacc ctgtcattca agcttgggca aaagcatatg gcgttattgc
    36421 ggatgtgttc atccaaattg aaaaagaaat ttatgatcaa atgatgtgga ttggttttaa
    36481 accattcaaa attaccaata ttaaacaaga atctgaagac attaaatcat ttacagttga
    36541 aactgaagaa tatgacttta gtgaatttac accaggccaa tacatcacag ttgatgtttc
    36601 tagtgataaa cttccatata gagctaaacg tcactattct atcgtatcag gtgaaaaaaa
    36661 ccatttaact tttggcgtta aacgtgatgt cacaacagaa catgaaggcg aagtttcaac
    36721 aattttacac gatgagatta aagaaggcga tatgattaat ttagctgcgc ctgtaggtgg
    36781 attcgtatta gagaatacga ctgaaccaca acttttctta ggttcaggta ttggtgttac
    36841 acctttagta gctatgtatg aagctgcctc tgccaaaggt ttagatacac agatggttca
    36901 agttgctgaa aatgaacaac atttaccttt caaagacaac ttcaacagta tcgcaagcca
    36961 tcatgacaac gctaaattat atacacactt aaaagataaa caaggctata ttggtgctga
    37021 agaattacaa gtatttttag caaataaacc tgaaatttat atctgtggtg gtacaaaatt
    37081 cttacaatct atgatcgaag cacttaaatc tttaaattac gatatggatc gcgtacacta
    37141 cgaaacattt attccaagac taagcgttgc agtataactg agaaaagagg tttagtgctc
    37201 atatgaaact taatcaacgt tacgtaaaag tatttgcatt atatttcgta agtattgtta
    37261 ctgcaaatat tattgttaaa aataataatt taattaaaac attgatacaa accatagccg
    37321 ggtacacggt ctttgcagtt ggtttgaagt atttaactaa acgtaaaaat aaatgacatc
    37381 tataccaaaa cagctatgac tttactttgt catggctgtt ttttaattgg gagtaggaca
    37441 gagatgatat tttcgcaaaa tttatttggt cgtcccacca caacatgcat tgatgtatgc
    37501 tcactgaatt tcataagaaa ggagttcaca agatgaccgt agatattgga cggatttatg
    37561 acaataaaga taataccgac gctattcgta tcctagtcga tagagtctgg ccgagaggta
    37621 tttcgaaaag aactgctaac ctagattatt ggttaaaaga cattgcccct tctactgagt
    37681 tgcgacaatg gttccaacat gatcctaaac tttttggagc ttttaaagaa aaatatgaaa
    37741 aagaattacg tgatcaggat gcgcaaaaag atgcttttga aaaattaaag gatattgtaa
    37801 atcagcataa tcatgttcta ttgttatatg cagcaaaaga tactaaacat aaccaagctg
    37861 tagtactaca gcagttgctc aatacttagt tatatatgtt tatcgtgaat cactataaat
    37921 gtcgctaact tcatttctaa tatttatttt taaaaagcat aattactaca attaattgaa
    37981 ctttaataat tactaaactt gaacaacatt ttactttaac aaaataaagt tttaaaatat
    38041 taattgttgg gttttacata gtaattagaa a
//
LOCUS       DANJFZ010000013        37753 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.013, whole genome shotgun sequence.
ACCESSION   DANJFZ010000013 DANJFZ010000000
VERSION     DANJFZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.013"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            14..967
                     /locus_tag="PWN45_000544"
     CDS             14..967
                     /locus_tag="PWN45_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622333.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1130..1333)
                     /locus_tag="PWN45_000545"
     CDS             complement(1130..1333)
                     /locus_tag="PWN45_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDL0622334.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1632..2120
                     /locus_tag="PWN45_000546"
     CDS             1632..2120
                     /locus_tag="PWN45_000546"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0622335.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2113..3048
                     /locus_tag="PWN45_000547"
     CDS             2113..3048
                     /locus_tag="PWN45_000547"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL0622336.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3045..4352
                     /gene="rsmB"
                     /locus_tag="PWN45_000548"
     CDS             3045..4352
                     /gene="rsmB"
                     /locus_tag="PWN45_000548"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL0622337.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4355..5449
                     /gene="rlmN"
                     /locus_tag="PWN45_000549"
     CDS             4355..5449
                     /gene="rlmN"
                     /locus_tag="PWN45_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL0622338.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5456..6199
                     /locus_tag="PWN45_000550"
     CDS             5456..6199
                     /locus_tag="PWN45_000550"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDL0622339.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6196..8190
                     /gene="pknB"
                     /locus_tag="PWN45_000551"
     CDS             6196..8190
                     /gene="pknB"
                     /locus_tag="PWN45_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDL0622340.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8418..9293
                     /gene="rsgA"
                     /locus_tag="PWN45_000552"
     CDS             8418..9293
                     /gene="rsgA"
                     /locus_tag="PWN45_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL0622341.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9294..9938
                     /locus_tag="PWN45_000553"
     CDS             9294..9938
                     /locus_tag="PWN45_000553"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL0622342.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9945..10586
                     /locus_tag="PWN45_000554"
     CDS             9945..10586
                     /locus_tag="PWN45_000554"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL0622343.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10967..11155)
                     /locus_tag="PWN45_000555"
     CDS             complement(10967..11155)
                     /locus_tag="PWN45_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL0622344.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11598..11972
                     /locus_tag="PWN45_000556"
     CDS             11598..11972
                     /locus_tag="PWN45_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0622345.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            11987..13633
                     /gene="fakA"
                     /locus_tag="PWN45_000557"
     CDS             11987..13633
                     /gene="fakA"
                     /locus_tag="PWN45_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDL0622346.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13823..15883
                     /gene="recG"
                     /locus_tag="PWN45_000558"
     CDS             13823..15883
                     /gene="recG"
                     /locus_tag="PWN45_000558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL0622347.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16086..16658
                     /gene="fapR"
                     /locus_tag="PWN45_000559"
     CDS             16086..16658
                     /gene="fapR"
                     /locus_tag="PWN45_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDL0622348.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16663..17649
                     /gene="plsX"
                     /locus_tag="PWN45_000560"
     CDS             16663..17649
                     /gene="plsX"
                     /locus_tag="PWN45_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL0622349.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17642..18568
                     /gene="fabD"
                     /locus_tag="PWN45_000561"
     CDS             17642..18568
                     /gene="fabD"
                     /locus_tag="PWN45_000561"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0622350.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18561..19295
                     /gene="fabG"
                     /locus_tag="PWN45_000562"
     CDS             18561..19295
                     /gene="fabG"
                     /locus_tag="PWN45_000562"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL0622351.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19538..19771
                     /locus_tag="PWN45_000563"
     CDS             19538..19771
                     /locus_tag="PWN45_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0622352.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            19887..20618
                     /locus_tag="PWN45_000564"
     CDS             19887..20618
                     /locus_tag="PWN45_000564"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL0622353.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20765..24331
                     /gene="smc"
                     /locus_tag="PWN45_000565"
     CDS             20765..24331
                     /gene="smc"
                     /locus_tag="PWN45_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL0622354.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24331..25581
                     /gene="ftsY"
                     /locus_tag="PWN45_000566"
     CDS             24331..25581
                     /gene="ftsY"
                     /locus_tag="PWN45_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL0622355.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            25568..25900
                     /locus_tag="PWN45_000567"
     CDS             25568..25900
                     /locus_tag="PWN45_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL0622356.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            25926..27293
                     /gene="ffh"
                     /locus_tag="PWN45_000568"
     CDS             25926..27293
                     /gene="ffh"
                     /locus_tag="PWN45_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL0622357.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27728..28003
                     /gene="rpsP"
                     /locus_tag="PWN45_000569"
     CDS             27728..28003
                     /gene="rpsP"
                     /locus_tag="PWN45_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL0622358.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28191..28694
                     /gene="rimM"
                     /locus_tag="PWN45_000570"
     CDS             28191..28694
                     /gene="rimM"
                     /locus_tag="PWN45_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL0622359.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28694..29431
                     /gene="trmD"
                     /locus_tag="PWN45_000571"
     CDS             28694..29431
                     /gene="trmD"
                     /locus_tag="PWN45_000571"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL0622360.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29534..29884
                     /gene="rplS"
                     /locus_tag="PWN45_000572"
     CDS             29534..29884
                     /gene="rplS"
                     /locus_tag="PWN45_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL0622361.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30128..32734)
                     /gene="yfhO"
                     /locus_tag="PWN45_000573"
     CDS             complement(30128..32734)
                     /gene="yfhO"
                     /locus_tag="PWN45_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDL0622362.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            33135..34019
                     /gene="ylqF"
                     /locus_tag="PWN45_000574"
     CDS             33135..34019
                     /gene="ylqF"
                     /locus_tag="PWN45_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL0622363.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34003..34770
                     /locus_tag="PWN45_000575"
     CDS             34003..34770
                     /locus_tag="PWN45_000575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL0622364.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            34879..36045
                     /gene="sucC"
                     /locus_tag="PWN45_000576"
     CDS             34879..36045
                     /gene="sucC"
                     /locus_tag="PWN45_000576"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL0622365.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36067..36975
                     /gene="sucD"
                     /locus_tag="PWN45_000577"
     CDS             36067..36975
                     /gene="sucD"
                     /locus_tag="PWN45_000577"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL0622366.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            37202..>37753
                     /locus_tag="PWN45_000578"
     CDS             37202..>37753
                     /locus_tag="PWN45_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HDL0622367.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTT"
BASE COUNT        14044 a         5098 c         7279 g        11332 t
ORIGIN      
        1 gaggagaaag tttatgaaaa agacactggg atgtttactt ttaattatgc ttttagtcgt
       61 agcaggttgt tcttttggtg ggaatcataa attatcatca aagaaatcag aagaatcaaa
      121 acaagaaact gtaaaaaaag aatcggaaga agagaaagat ccagatttag agaaatatga
      181 agaaatagag aagaaaatga aaggaattaa agatgcgcca tctcttgata agttggatcc
      241 attaatgaca gaaaagtcgt ttacgaatag taaagggatt caaggatgga aagattacaa
      301 agaattaatg ggtaaagtgg aacttgcaga ttatagattt actaaagatt caaaaggatc
      361 ttcaataaaa gatgttgatg cattctttaa aggtaagaaa ggtataaaaa ggaaagtgat
      421 tgaaacacac gatgatgtaa aacaagttga ttattggtat gtagatccag atggaaagaa
      481 aattggcaat tcaaacacac ctgtttttta cgcagaaatt atgacaaaat ataaagatgg
      541 aaagttagtt tatgcatcag tcgaaccagg atcttacgta atacataaag atgatgcaat
      601 taaatatgac gattattcta agttaaaaaa attaagtcag ctaactaaac ttgatcatcc
      661 aaaaccagtt ccatatagcg tagctcaaat caaatctttc ggagtacctt taacaagcgt
      721 ttcatttatg acacatggat caaaggatac taaagatgaa gtgttgccgg cattggccta
      781 tttcactttt tcaccaaaaa attatgaaga caagtctaat ccagatccaa aagttttaaa
      841 tttagtacat atggatttct taaatgcatc tagtgatttt ggtaacgcac attttgttgt
      901 tttaagtaaa tatattaaag agtatgaatc aaactatgaa acagcgtcag atgattcttt
      961 aaaatagtat ttactgtgtg aaaaataaat agtgtactac attaaataat cgcaataata
     1021 atcccgataa acaatcagca ttactgctta tcacatagag ttcgtaataa ctataactct
     1081 atgattcgca aataataaat gattgtcatc gggatttatt tttatcaatt tataaagtga
     1141 cattaccttg ttcatcagca ggtttgaaaa cagtaatcac tgcactaata attgctaaaa
     1201 tgtgtgggat acctgtccaa cagaatatta agtgtagaat accttgcata ttcttgccgg
     1261 cataaaattt atgaatacca aaactaccta agaacaatgc taataaaata taaataactt
     1321 tgtttacttg catttctttc cctccagttg aattgcttat aatgacatta gcttctcttt
     1381 ttattatacc cacttttagt tcaaacattc tagtttaagc attcccaatc atctaaattt
     1441 cagttattca atccttacaa taaatttagg attacatttc agttgcattg tattatttta
     1501 cgtgtgaaat atacgtaatg aatcacatga caatcttcaa attgaaaaat atacattcta
     1561 tgatgtaagg tcgcattttt aatatattta cgttataata gtttgatgaa ttttattgag
     1621 gagtaagagt tatggcgatt aaaaagttag taccagcatc gcatcctatt ttaacgaaaa
     1681 aagcgcaagc agttaaaaca tttgatgatt cgttaaaaag attattacaa gatttagaag
     1741 atacaatgta tgcacaagaa gctgctggct tatgtgcacc tcaaattaat cagtcattgc
     1801 aagtggcaat tattgatatg gaaatggaag gattattaca acttgttaat ccgaaaatta
     1861 ttagtcaatc aaatgaaaca ataacagact tagaaggttc aattacattg ccagatgttt
     1921 acggcgaagt gacaagaagt aaaatgatag ttgtcgaaag ttatgacgtc aatgggaaca
     1981 aagttgaact aactgcacat gaagatgtag caagaatgat tttgcatatt atagatcaaa
     2041 tgaacggtat cccttttaca gaacgtgcgg accgtatttt aacagataaa gaagtggagg
     2101 catattttat aaatgactaa aataatattt atgggtacac cagacttttc aacaactgtt
     2161 ttagaaatgc ttattgcaga acatgatgtc attgcagtcg taacgcaacc agatcgacct
     2221 gttggacgta aacgtgttat gacaccacca ccagttaaaa aagttgcaat gaaatatgat
     2281 ttacctgtat atcaacctga aaaattaagt ggatcagaag aattagaaca attgcttcaa
     2341 ttagatgtag atttaattgt aactgctgct tttggacaat tattacctga atcattgttg
     2401 gcattaccaa atcttggggc aattaatgta catgcatcat tgttaccgaa gtatagaggt
     2461 ggtgcaccaa ttcatcaggc aattatcgat ggtgaacaag aaaccggcat aacaattatg
     2521 tatatggtta aaaaattaga tgcgggtaat attatttcgc aacaagcaat taaaatagaa
     2581 gaaaatgata atgtcggtac gatgcatgat aaattaagtg tattaggggc agatttatta
     2641 aaagaaactt taccatctat tatagagggc acaaatgaaa gcgtacctca agatgatacg
     2701 caagcaacat ttgcttccaa tattcgacgc gaagatgagc gaattagctg gaataaacca
     2761 ggaagacaag tgtttaatca aattcgtgga ttatcaccat ggccagttgc ttatacaact
     2821 atggatgaca ctaacttgaa aatatacgat gctgaactcg ttgagactaa taagataaac
     2881 gagcctggaa ccattataga aacgactaaa aaagccatta ttgttgctac aaatgataat
     2941 gaagctgttg caattaaaga tatgcaatta gctgggaaaa agagaatgtt agctgccaat
     3001 tatttaagtg gtgcgcaaaa cacactagta gggaagaaac ttatatgata gaaaacgtga
     3061 gaagtcttgc ttttgacacg attcaagata tattaaatga aggtgcgtat agtaacttgc
     3121 gtatcaatga agtgttgtca gaaaatgaat taaatgcaat ggataaggct ttatttacag
     3181 aaattgtcta cggaaccgtt aaaagaaaat atacgttaga tttttattta aagccttttg
     3241 tgaaaacaaa gattaaggca tgggttaggc aattattatg gatgagtatt tatcaatatg
     3301 tttatttaga taaagttcca aatcatgcca ttattaatga agcagttgaa atagcaaaag
     3361 aacgcggtgg ctatcataat ggtaatgtcg taaatggtat tttacgtaca atgatgcgta
     3421 gtgacttacc tgattttaat gaaattgcag atcctaaaaa aagaatggca atcgagtata
     3481 gtatgccgaa gtggattata gatcattggg caacacatta tggtctcgaa gaaactgaaa
     3541 caattttaca gtcattttta gaaacgacat caacaactgt gcgtgccaac ctgacgcgag
     3601 catcattaga tgatattatt gaaaagttgc aagacgaagg ttatgacgtt gaaaaagatc
     3661 atgacttacc ttattgtctc catataggag gacaaccaat tattcattct cgttcattta
     3721 aagatggatt cgtttcaatt caagataaaa gctcaatgtt tgttgcacac attatgaatg
     3781 tagaccgaca tgatcacgta ttagatgcat gtagtgcacc tggcggtaaa gcttgtcaca
     3841 ttgctgaagt tttaatgcca gaagggcaag ttgacgcttc agatatacat gatcacaaaa
     3901 tagacttaat taattttaat ataaaaaaat tacgattaac aaatattaaa gcttttcaac
     3961 atgatgcgac aaaaccttat gataaaacat acgataagat acttgttgat gcaccatgta
     4021 gcggattagg tgtaatgaga cataagccgg agattaagta tactcaaagc aaacaacata
     4081 ttgagtcact agttgaatta cagcttgaaa tattggaaaa tgtaaaaaac aatgtaaaaa
     4141 taggtggcga aatcatctat tcaacatgta caattgagca actagaaaat gaaaacgtga
     4201 tttatacgtt tttgaaaaat aataaaaact tcgaatttga accgtttcaa catccgataa
     4261 ctggagagtt ggtcaaaacg ttacaaatca tgccgcaaga ctttaattca gatggattct
     4321 ttatcactaa gataaaaaga aaggacaatt aggaatgata actgctgaaa agaaaaagaa
     4381 gaataaattt cttccaaatt ttgacaagca atcaatatat tcattgcgat ttgacgaaat
     4441 gcaaaactgg ctcgttgaac aagggcaaca aaaatttcga gcgaaacaga tttttgaatg
     4501 gttatatcaa aaaagagtag attcgattga tgaaatgacg aacttatcga aagacttacg
     4561 acagctttta aaagataact ttactgttac aactttaaca actgtagtaa aacaagaaag
     4621 taaagacggt acaattaaat tcttatttga attacaagat ggctatacaa ttgaaactgt
     4681 tttaatgaga catgattatg gaaattcagt atgtgtaacg acacaagtag gttgtcgcat
     4741 cggatgtacg ttttgtgctt ctacacttgg cggcttaaaa agaaaccttg aagctggcga
     4801 aattgtttca caagttttaa cagttcaaaa agcccttgat gctacagaag agcgcgtatc
     4861 tcaaattgtc ataatgggta tcggtgaacc atttgaaaat tatgatgaaa tgatggactt
     4921 tttaagaatc gtcaatgatg ataacagttt aaatattggt gcacgtcaca ttacagtatc
     4981 aacatcaggt atcattccta gaatatatga ctttgcggat gaagatattc aaattaattt
     5041 tgctgtaagc ttacacgccg caaaagatga agtgcgatca cgcttgatgc caattaaccg
     5101 tgcatataat gttgagaagt taatcgaagc aattcaatat tatcaagaaa aaacaaatcg
     5161 tcgtgttact tttgaatatg gtctgtttgg tggtgtgaat gaccaactag aacatgcaag
     5221 agaattagca catttaataa aaggcttaaa ctgccatgtt aacttaattc ctgtcaacca
     5281 tgttccagaa agaaattatg tgaaaacggc taaaaatgat atctttaaat ttgaaaaaga
     5341 attaaagaga ctaggaatta atgccacaat acgtcgtgaa caaggttcgg atattgacgc
     5401 agcttgtggt caattaagag caaaggaacg acaagtagaa acgaggtaaa gacaaatgct
     5461 agaggcacaa ttttttactg atactggaca acatagagat aagaatgaag atgcgggtgg
     5521 tattttttat aatcaaacta atcaacaact tttagttctg tgtgatggta tgggtggcca
     5581 taaagcagga gaagttgcaa gtaaatttgt tacagatgag ttgaaatccc gttttgaagc
     5641 ggaaaatctt atagaacaac atcaagctga aaattggttg cgtaataata taaaagatat
     5701 aaattttcag ttatatcact atgcacaaga aaatgcagaa tataaaggta tgggtacaac
     5761 atgtgtttgt gcacttgttt ttgaaaaatc agttgtgata gcaaatgtcg gtgattctag
     5821 agcctatgtt attaatagtc gacaaattga acaaattact agtgatcact catttgttaa
     5881 tcatcttgtt ttaacgggtc aaattacgcc ggaagaagca tttacacatc cacaacgtaa
     5941 tattattacg aaggtgatgg gcacagataa acgtgtgagt ccagatttgt ttattaagcg
     6001 attaaatttt tatgattatt tattattaaa ttcagatgga ttaactgatt atgttaaaga
     6061 caatgaaatt aagcgtttgt tagtaaaaga aggtacaata gaagatcatg gtgatcaatt
     6121 aatgcaattg gcattagata accattcgaa agataacgtt actttcatac tcgcggctat
     6181 tgaaggtgat aaagtatgat aggtaaaata ataaatgaac gatataaaat tgtagataag
     6241 cttggcggcg gtggcatgag taccgtttat cttgctgaag atacgatact taacattaaa
     6301 gttgcaatta aggcgatttt tataccacct agagaaaaag aagaaacatt aaaacgtttt
     6361 gaacgagaag tacataactc atcacagcta tcacatcaaa atatagtaag tatgatcgat
     6421 gttgatgaag aagatgactg ttactactta gtaatggaat atatcgaagg tccgactttg
     6481 tctgagtata ttgaaagtca tgggccatta agtgttgaca cagcgattaa ttttacgaat
     6541 caaatattgg atggcattaa acatgcgcat gatatgcgta ttgtacatag agatattaag
     6601 ccacaaaata tattaattga cagcaataaa acgttgaaaa tatttgattt tggaattgct
     6661 aaagctttaa gtgagacgtc tttaactcag actaatcatg tgttaggtac tgtgcagtac
     6721 ttttcgccag aacaagcaaa aggtgaggca acggatgaat gtacagatat ttattctata
     6781 ggtattgtgt tatatgaaat gcttgttggt gaaccaccct ttaatggaga aactgcagtt
     6841 agcattgcga ttaaacatat tcaggattct gtgccaaatg tgacaacaga tgtacgtaag
     6901 gatattccgc aatctttaag taatgtcatt ttacgcgcta cagaaaaaga caaagcgaat
     6961 cgttacaaaa caattcaaga aatgaaagat gatttgagta gtgttttaca tgaaaatcga
     7021 gcgaatgaag atgtctatga actcgataaa atgaaaacga tagcggtacc tttgaaaaaa
     7081 gaagatctag caaagcatat tagtgaacat aagtcgaatc aacctaaacg tgaaacgacg
     7141 caagtaccta ttgtaaatgg gcctgctcat catcagcaat tccaaaagcc agaaggtacg
     7201 gtgtacgaac caaaacctaa aaagaaatca acacgaaaga ttgtgctctt atcactaatc
     7261 ttttcgttgt taatgattgc acttgtttct tttgtggcaa tggcaatgtt tggtaataaa
     7321 tacgaagaga cacctgatgt aatcgggaaa tctgtaaaag aagcagagca aatattcaat
     7381 aaaaacaacc tgaaattggg taaaatttct agaagttata gtgataaata tcctgaaaat
     7441 gaaattatta agacaactcc taatactggt gaacgtgttg aacgtggtga cagtgttgat
     7501 gttgttatat caaagggccc tgaaaaggtt aaaatgccaa atgtcattgg tttacctaag
     7561 gaggaagcct tgcagaaatt aaaatcgtta ggtcttaaag atgttacgat tgaaaaagta
     7621 tataataatc aagcgccaaa aggatacatt gcaaatcaaa gtgtaaccgc aaatactgaa
     7681 atcgctattc atgattctaa tattaaacta tatgaatctt taggcattaa gcaagtttat
     7741 gtagaagact ttgaacataa atcctttagc aaagctaaaa aagccttaga agaaaaaggg
     7801 tttaaagttg aaagtaagga agagtatagt gacgatattg atgagggtga tgtgatttct
     7861 caatctccta aaggaaaatc agtagatgag gggtcaacga tttcatttgt tgtttctaaa
     7921 ggtaaaaaaa gtgactcatc agatgtcaaa acgacaactg aatcggtaga tgtaccatac
     7981 actggtaaaa atgataagtc acaaaaagtt aaagtttata ttaaagataa agataatgac
     8041 ggttcaactg aaaaaggtag tttcgatatt actagtgatc aacgtataga cattccttta
     8101 agaattgaaa aaggaaaaac agcaagttat attgttaaag ttgacggtaa aactgtagct
     8161 gaaaaagaag tcagctatga tgatgtataa atataattga agtaaatgta ccgaggtttc
     8221 tatttggaag tctcggtatt tttatgttga gattgcggta gttttaaaat gcttcatgtc
     8281 atcatataca ggtgagcatt gtctatcaat tctgtatcac tctcacaata tttatatcat
     8341 ttgaaatgat catttatata ttaattttcc agtctttgat ataataaatg cagtaaaatt
     8401 aatcgagagg tgccattttg aagacaggtc gaatagtgaa atcaattagt ggggtatatc
     8461 aagtagacgt taatggcgaa cgtttcaata caaaaccacg aggattattt agaaagaaaa
     8521 aattttcacc ggtagttggt gatatagtgg aatttgaagt tcaaaacatt aacgaaggtt
     8581 atattcatca agtgtttgag cgggaaaatg agttgaaaag accacctgta agtaatatag
     8641 atacactagt aattgtaatg agtgctgtcg agccaaattt ttcaacgcaa ttattagatc
     8701 gatttttagt tattgcacat tcgtatcagt taaatgcgag aattttggtg actaaaaaag
     8761 ataaaacacc aattgaaaag cagttcgaaa taaatgagtt gttgaaaata tatgaaaata
     8821 ttggctatga gactgaattt attggaaatg atgatgatcg aaaaaaaatt gtagaagctt
     8881 ggccagctgg acttatagta cttagtggtc aatcaggtgt cggtaagtcc actttcttaa
     8941 atcattatcg tccagaactt aatcttgaga caaatgatat atcaaaatca ttaaatcgag
     9001 gaaagcatac tacaagacat gtcgaactat tcgaacgtca aaacggttat attgcagaca
     9061 cacctggatt cagtgcttta gattttgatc atatagataa agatgaaata aaagattatt
     9121 ttcttgaatt aaatcgatat ggtgaaacat gtaagtttag gaattgtaat catatcaaag
     9181 aacctaattg taatgttaag catcaattag agatagggaa tattgcgcaa tttagatacg
     9241 accattattt acaactattt aatgaaattt caaatagaaa ggttagatat taaatgacaa
     9301 aactatatcc atcattatta tctgttgatt ttttggattt acaacatgaa ttaaaacgac
     9361 ttgaagaagc aggcgtcgac ggagttcatt ttgatgttat ggatggtcaa tttgtgccta
     9421 atatatctat tggtttacca atattagatg cagtaagaaa aggcacaaca ttacctatag
     9481 acgtacattt gatgattgaa aatccagaaa agtatattgc atcatttgca gaacatggtg
     9541 ccgatatgat ttcaattcat gtcgaatcaa cgcctcatat tcatcgtgct attcaaatga
     9601 ttaaacattt agataaaaaa gctggtgtag taattaatcc tggtacacca atatcacaaa
     9661 ttgaacctat tttagacatt gttgattatg tactagtgat gacagttaac ccagggtttg
     9721 gtggtcaatc atttattgat caatgcgtag aaaaaatagc gggtcttaat gctattaaaa
     9781 tggaacgtca attaaacttt gatattgaag ttgatggagg cgtaaatacc gatacagcga
     9841 aagtttgtgt tgaaaatggt gctacaatgc tagtaacagg ttcatttttc tttaaacaag
     9901 aggattataa aaaagtcaca caagcattga aaggttgagt gtatatgcat ataaatttat
     9961 tatgttctga tcgacacttg ccgcaagata tttgggccaa aagtaatgaa ggtaaatggg
    10021 gcggcgttga tagaggtgct ttgattttat tgaagcatca aattatccct tttttctcag
    10081 tgggagactt tgattcagtc agtaaagaag aacgccaact tctaacagaa cagttacaaa
    10141 tcaaaccagt tcaagctgaa aaagctgata cggatttagc tttagcggtt gataaagctg
    10201 ttgcacttgg atttgatagt attacaattt atggtgcaac aggcggacga ttagatcact
    10261 tttttggggc aattcagtta ttattgaaaa aagcatatta taaacatgat gttcatatag
    10321 aagttatcga tcaacaaaat aaaattgaat tattgcctaa aggtcaacat acagttgaaa
    10381 aagataagag ttatccgtac atttcattta taccgatgac tgatgatgta gaactttctc
    10441 tagcaggttt taaatataat ttagctagac aaatgcttaa tataggttct actttaacta
    10501 tttcaaatga aattgagtct ttgcaagcga aagtaactgt acatgatggg ttgattttgc
    10561 aaattagaag tacagattta aattaagtat attcaaattt gtgaggattt ttaataaagt
    10621 gcatgagtta tttaataaag tgacgtttat agatagaatt tataacataa taatagatga
    10681 atcacgctgt aattaatgaa gattatggcg tgatttatta ttgaaaggaa aattgtaggt
    10741 cctagtacat tgatttatgt gccaaaatcg atattgaacc cataagtcca atttgacaga
    10801 gaaccattat taaacgtatt aaccaaatta tattgatagt tcgaatttca ctatgaacca
    10861 ttatgtattg tttggtaaca aaaaaagaac taagaggttg agctcttagt tcttaagtaa
    10921 atgtgtcctc cacataaggt cacatttttt atgtgcaata tatttattaa actctagtta
    10981 ctttaccaga ttttaaagca cgtgcagaaa cccaaacttt tttaggttta ccgtcaacta
    11041 ggattctaac tttttgaagg ttagcgttcc atctacgttt agtagagttt aaagcgtgtg
    11101 aacgtctgtt accagtcgaa gctttacgac ctgttacgaa acattgttta cccatatgag
    11161 tacctccttt aataaatata aatacacata actacttata tacttaatta agatagcata
    11221 gtttcatttg aaaaacaatg aataattttc acataaaagt caaaaatact gagttttgtg
    11281 atataattgt agactgtgaa gttatgtagt atgatatttt agagaaataa gtgaatgaaa
    11341 catttaaaat ttttatttaa tgatactaca tctattaaaa gaaaacgcct atagataatc
    11401 gtcatgagtg taaattcagt ttactcaaaa tataataggt gatttaagcg taagtcattc
    11461 tgataagttg taccattgat attttataaa aataatcaat gaaacttaaa gcgtttatga
    11521 ctacactaag tggtaaaatt ttaaaagtga ttagggaaaa cgaataataa actttgaaat
    11581 ttaggaggga caaagatatg acattagaga tttcaaatga ttacggtaaa attgatattt
    11641 caaacgaagt gattgcttcg gttgtaggtg gaaaggccgt tgaatgttat ggtattgtag
    11701 gtatggcatc tagacaacaa gttagagatg gtattgcgga aatactagga catgaaaatt
    11761 atgctaaagg catcaaagta actgaaaata atggcgtagt ggatatagat atgtacatta
    11821 ttgtaagtta cggtgtgaaa atatctgaag ttgccaataa tgtacaatca acagtgaaat
    11881 atactttgga aaaatcactt aatgtatcag taaattcaat caatatatat gtacaaggtg
    11941 tacgtgtgaa taatacaggc aagaaagctt aggaggacaa cttgaaatga ttagcaaaat
    12001 taatggtaaa ttatttgccg atatgattat acaaggggca caaaatttat ctaacaatgc
    12061 agatttggta gattctttga atgtgtatcc agtgccagat ggtgatacag gaacaaatat
    12121 gaatcttact atgacttcag gtcgcgaaga agtagagaat aatttgtcga aaaatatcgg
    12181 cgaattaggt aaaacattct cgaaaggttt actaatgggt gcaagaggta actctggtgt
    12241 catcttgtca caattattca gaggattttg taaaaatatt gaaagtgaat ctgaaattaa
    12301 ttcaaaattg ttagctgaaa gttttcaagc tggtgttgaa acggcatata aagctgttat
    12361 gaaaccagtt gaaggtacaa tacttacagt tgcaaaagat gctgcgcaag ctgcaataga
    12421 aaaagcaaat aatactgaag attgtataga attaatggag tacattattg taaaagccaa
    12481 tgaatcactt gaaaacacac caaacttatt agctgtactt aaagaagttg gtgttgttga
    12541 tagtggcggt aaaggtttgt tatgcgttta cgaaggattc ttaaaagcgc ttaaaggtga
    12601 aaaagttgaa gccaaagttg caaagataga taaagatgaa tttgtacatg atgaacatga
    12661 tttccatggt gtaattaata ctgaagatat tatttatggc tattgtactg aaatgatggt
    12721 tcgttttgga aagaataaaa aagcctttga tgaacaagaa ttcaggcaag atatgagtca
    12781 atttggtgat tctttattag tcattaatga tgaagaaatt gtgaaagttc acgtgcatac
    12841 cgaataccca ggtaaagtgt ttaattatgg tcaacaatat ggtgaattaa ttaaacttaa
    12901 ggttgaaaat atgagagaac agcaccgtga agtgattcga aaagaacagc acacagctaa
    12961 accgaaaatg gaaacggttg aaacagcaat tattactatt tctatgggtg aaggtatttc
    13021 agagatattt aaatcaatgg gtgccacaca tatcattagt ggtggacaaa cgatgaatcc
    13081 ttctacagaa gatatcgtta aagtcattga acaatcaaaa tgtaaacgtg caattatttt
    13141 accgaataat aaaaatatct taatggcaag tgaacaagca gcgagtattg ttgatgcaga
    13201 agctgttgtt attccaacga aatctattcc tcaaggtata agcgcactat tccaatatga
    13261 tgtggacgca acacttgaag aaaataaagc gcaaatggct gattcagtaa ataacgttaa
    13321 atctggttca ttaacgtacg ctgttcgtga tacgaaaatt gatggcgttg agattaaaaa
    13381 agacgcgttt atgggcttga ttgaagataa gattgtaagc agccaaagtg atcaattaac
    13441 aacggttact gagttgttaa atgagatgtt agcagaagat agtgaaatat tgactgtgat
    13501 tattggtcaa gatgcagagc aagcagttac agataacatg ataaactgga tcgaagagca
    13561 atatccagat gtagaagtgg aagttcatga aggtggacaa ccaatttatc aatatttctt
    13621 ttcagtagaa taaaaattta aaataaaaaa ctaccaatga taaatcatca gttggtagtt
    13681 ttttattttg ctattttagt gatattgcgg gttaaaagta tcgttctcga gttgctaaca
    13741 atgtcatgtt caacttagtc atgataaaat aaataacata ctaaatgata cgtaaaatca
    13801 aataaaacat aggtgattta ttttggctaa agtaaactta atagaaagtc catattctct
    13861 tttacaatta aaaggtatag gtcctaagaa aatagaagta ttgcaacaac taaatattca
    13921 tacagtggaa gatcttgttc tttatttgcc aactagatat gaagataata cagtgattga
    13981 tttgaatcaa gcagaagatc aatctaacgt tacgatagaa ggacaagtat atacagctcc
    14041 agtagttgca ttttttggaa gaaataaatc aaaattaacc gttcatttaa tggtaaataa
    14101 tattgctgtc aaatgtattt ttttcaatca accgtattta aaaaagaaaa tcgaattaaa
    14161 tcaaactata actgttaaag gtaagtggaa tagggttaaa caggaaatta ctggtaatag
    14221 ggttttcttt aattcacaag ggacacaaac tcaagaaaac gcagatgttc aattagaacc
    14281 agtctatcgt attaaggaag gtattaaaca aaagcaaata cgagaccaaa ttagacaagc
    14341 gttaaatgat gtgacaattc atgaatggtt aactgatgaa ctaagagaaa aatataaatt
    14401 agagaccttg gactttactt tgaacacatt acatcatcct aaaagtaaag aggatttatt
    14461 acgtgctcgt agaacctatg catttactga actgttttta ttcgaattac gtatgcaatg
    14521 gctaaataga ttagaaaagt catctgacga agcaattgaa attgattatg acatagacca
    14581 agttaaatca tttattgatc gtttaccttt tgaactaact gaagcacaga aatccagtgt
    14641 taatgaaatt tttagagatt taaaagcacc aatacgtatg catcgattac ttcaaggtga
    14701 tgtaggttca ggaaaaacag tagttgctgc aatttgtatg tatgcgttaa aaactgctgg
    14761 ttatcaatca gcattgatgg taccaactga aattttagca gagcaacatg ctgaaagttt
    14821 aatggcttta tttggagatt ctatgaacgt tgcattgtta actgggtcag taaaaggtaa
    14881 gaaacgaaag atacttttag aacaacttga aaatggtacg attgattgtt taattggaac
    14941 ccatgctttg attcaagatg atgtgatttt ccataatgtt ggtttagtaa ttacagatga
    15001 acaacatcga tttggtgtga atcaacgcca gcttttaaga gaaaaaggtg caatgacgaa
    15061 tgtgttattt atgacagcaa cgccgatacc aagaacacta gcaatatcag tttttggtga
    15121 gatggatgtg tcttcaatta aacaattacc aaaaggtcgt aaacctatca ttactacttg
    15181 ggcaaagcat gagcaatacg ataaagtttt gatgcaaatg acctcagagt tgaaaaaagg
    15241 tcgtcaagca tatgtcattt gcccgctaat agaaagttct gagcatctcg aagatgttca
    15301 aaatgttgtc gcattgtacg agtctttaca acagtattat ggtgtttccc gtgtagggtt
    15361 attgcatggt aagttatctg ccgatgaaaa agatgaggtc atgcaaaagt ttagtaatca
    15421 tgagataaat gttttagttt ctactactgt tgttgaagta ggtgttaatg taccgaatgc
    15481 aacttttatg atgatttatg atgcggatcg ctttggatta tcaactttac atcagttacg
    15541 cggtcgtgta ggtagaagtg accagcaaag ttactgtgtt ttaattgcat cccctaaaac
    15601 agaaacagga attgaaagaa tgacaattat gacacaaaca acggatggat ttgaattgag
    15661 tgaacgagac ttagaaatgc gtggtcctgg agatttcttt ggtgttaaac aaagtggatt
    15721 gccagatttc ttagttgcca atttagttga agattatcgt atgttagaag ttgctcgtga
    15781 tgaagcagct gaacttattc aatctggcgt attctttgaa aatacgtatc aacatttacg
    15841 tcattttgtt gaagaaaatt tattacatcg tagttttgac taattgccat gctgatttgt
    15901 caatttgagt gcaacacttc gttaattgag tgatatgaca cttgaactat ttaaatgtaa
    15961 agtggtattt taacaattta taaattttcg actaaataat agctaaatat tacagttatt
    16021 tgttgagtcg gttaaataga aagtgttatg atatgtgagg aatgtttaag actaggtact
    16081 aaaaaatgag gggtgagacg ttgaaactaa agaaagataa acgtagagaa gcaatcagac
    16141 aacaaattga tagcaatccc ttcatcacag accatgaact aagcgactta tttcaagtga
    16201 gtatacaaac aattcgttta gatcgcactt atttaaacat accagaatta aggaagcgta
    16261 ttaaattagt tgctgaaaag aattatgacc aaataagttc tattgaagaa caagaattta
    16321 ttggtgattt gattcaagtc aatccaaatg ttaaagcgca atcaatttta gatattacat
    16381 cggattctgt ttttcataaa actggaattg cgcgtggtca tgtgctgttt gctcaggcaa
    16441 attcgttatg tgttgcgcta attaagcaac caacagtttt aactcatgag agtagcattc
    16501 aatttattga aaaagtaaaa ttaaatgata cggtaagagc agaagcacga gttgtaaatc
    16561 aaactgcaaa acattattac gtcgaagtaa agtcatatgt taaacataca ttagttttca
    16621 aaggaaattt taaaatgttt tatgataagc gaggataaaa ttatggttaa attagcaatt
    16681 gatatgatgg gtggcgacaa tgcgcctgat atcgtattag aagccgtaca aaaggctgtt
    16741 gaagacttta aagatctaga aattatactt ttcggtgacg aaaaaaagta taatctgaac
    16801 catgaacgaa tcgaatttag acattgttct gaaaagattg aaatggaaga tgagcctgtt
    16861 agagcgatta aacgtaaaaa agatagctca atggtaaaaa tggctgaagc tgtgaaatct
    16921 ggtgaagcag atggatgtgt gtcagcaggt aatactggtg ctttaatgtc agctggttta
    16981 ttcattgttg gacgtattaa aggtgtagct agaccggctt tagtagtaac attgccaacg
    17041 attgatggaa aaggttttgt ctttttagac gttggtgcaa atgctgatgc taaacctgaa
    17101 cacttattac agtatgcgca actaggggat atttatgctc aaaaaattag aggtattgat
    17161 aatccgaaaa tctcattatt aaatatagga accgagccag ctaaaggtaa tagtttaacg
    17221 aaaaaatcat atgagttatt aaatcatgat cattcattga attttgttgg gaatattgaa
    17281 gcgaagacat taatggatgg cgatacagat gttgtagtta ccgatggcta tactgggaac
    17341 atggtcctta aaaatttaga aggtactgca aaatcaatcg gtaaaatgtt aaaagatacg
    17401 attatgagta gtactaaaaa taaattagca ggtgcaatat tgaagaaaga tttagctgaa
    17461 ttcgctaaaa agatggatta ctcagaatac ggtggttccg tattattagg attggaaggt
    17521 actgtagtta aagcacacgg tagttcaaat gctaaagctt tttattctgc aattagacaa
    17581 gcgaaaatcg caggagaaca aaatattgta caaacaatga aagagactgt aggtgaatca
    17641 aatgagtaaa acagcaatta tttttccggg acaaggtgcc caaaaagttg gtatggcgca
    17701 agatttgttt aacaacaatg atcaagcaac tgaaatttta acttcagcag cgaacacatt
    17761 agactttgat attttagaga caatgtttac tgatgaagaa ggtaaattgg gtgaaactga
    17821 aaacacacaa ccagctttat tgacgcatag ttcggcatta ttagcagcgc taaaaaattt
    17881 gaatcctgat tttactatgg ggcatagttt aggtgaatat tcaagtttag ttgcagctga
    17941 cgtattatca tttgaagatg cagttaaaat tgttagaaaa cgtggtcaat taatggcgca
    18001 agcatttcct actggtgtag gaagcatggc tgcagtattg ggattagatt ttgataaagt
    18061 cgatgaaatt tgtaagtcat tatcatctga tgacaaaata attgaaccag caaacattaa
    18121 ttgcccaggt caaattgttg tttcaggtca caaagcttta attgatgagc tagtagaaaa
    18181 aggtaaatca ttaggtgcaa aacgtgtcat gcctttagca gtatctggac cattccattc
    18241 atcgctaatg aaagtgattg aagaagattt ttcaagttac attaatcaat ttgaatggcg
    18301 tgatgctaag tttcctgtag ttcaaaatgt aaatgcgcaa ggtgaaactg acaaagaagt
    18361 aattaaatct aatatggtca agcaattata ttcaccagta caattcatta actcaacaga
    18421 atggctaata gaccaaggtg ttgatcattt tattgaaatt ggtcctggaa aagttttatc
    18481 tggcttaatt aaaaaaataa atagagatgt taagttaaca tcaattcaaa ctttagaaga
    18541 tgtgaaagga tggaatgaaa atgactaaga gtgctttagt aacaggtgca tcaagaggaa
    18601 ttggacgtag tattgcgtta caattagcag aagaaggata taatgtagca gtaaactatg
    18661 caggcagcaa agagaaagct gaagcagtag tcgaagaaat caaagctaaa ggtgttgaca
    18721 gttttgcgat tcaagcaaat gttgccgatg ctgatgaagt taaagcaatg attaaagaag
    18781 tagttagcca atttggttct ttagatgttt tagtaaataa tgcaggtatt actcgcgata
    18841 atttattaat gcgtatgaaa gaacaagagt gggatgatgt tattgacaca aacttaaaag
    18901 gtgtatttaa ctgtatccaa aaagcaacac cacaaatgtt aagacaacgt agtggtgcta
    18961 tcatcaattt atcaagtgtt gttggagcag taggtaatcc gggacaagca aactatgttg
    19021 caacaaaagc aggtgttatt ggtttaacta aatctgcggc gcgtgaatta gcatctcgtg
    19081 gtatcactgt aaatgcagtt gcacctggtt ttattgtttc tgatatgaca gatgctttaa
    19141 gtgatgagct taaagaacaa atgttgactc aaattccgtt agcacgtttt ggtcaagaca
    19201 cagatattgc taatacagta gcgttcttag catcagacaa agcaaaatat attacaggtc
    19261 aaacaatcca tgtaaatggt ggaatgtaca tgtaatatat ttgagctaaa gctcattgac
    19321 gcagtggttg actggtcatc caatggagaa ttgtctgacc tagtcaactt tgcgggggaa
    19381 attctaagca acctagataa ggttccagaa tttctcccta agaaacacta atcaataaat
    19441 tgataagtgt ttctaaaatt tctacttgtt ttttagaatt taaaatggga aaatatagta
    19501 gtctatgtat aggcattttt aaaggaggtg aatcgacgtg gaaaatttcg ataaagtaaa
    19561 agatatcatc gttgaccgtt taggtgtaga cgctgataaa gtaactgaag atgcatcttt
    19621 caaagatgat ttaggcgctg actcacttga tatcgctgaa ttagtaatgg aattagaaga
    19681 cgagtttggt actgaaattc ctgatgaaga agctgaaaaa atcaacactg ttggtgatgc
    19741 tgttaaattt attaacagtc ttgaaaaata ataaatctta catctgggtc gtcagtattg
    19801 tcgactcagt ttttttcttt aattatcaat agttttaacg taaaattaaa gatgattcaa
    19861 gagcaacaca taaaggagat aaaataatgt ctaaacaaaa gaaaagtgag atagttaatc
    19921 gttttagaaa gcgctttgat actaaaatga cagagttagg ctttacttat caaaatattg
    19981 atttatacca acaagcattt tcgcattcga gttttattaa tgattttaat atgaatcgtt
    20041 tagaccataa tgagcgttta gagtttttgg gtgatgcggt attagaattg acggtttcac
    20101 gatatttatt tgataaacat cccaacttgc cagaagggaa tttaacaaaa atgcgtgcca
    20161 ctattgtatg tgagccctca cttgtaatat ttgcgaataa aattggattg aacgaaatga
    20221 ttttacttgg taaaggtgaa gagaaaacag ggggacgtac aagaccatca ttaatatcag
    20281 atgcattcga agcatttatt ggggcattgt atttggatca aggactagat atagtttgga
    20341 aatttgctga gaaagtcatt ttcccacatg tagaacaaaa tgagttatta ggcgtggtag
    20401 attttaaaac acaattccaa gaatatgtgc accagcaaaa taaaggtgat gtaacctata
    20461 atttaataaa agaagaggga ccggcacatc atcgtctatt cacttcagaa gttattctgc
    20521 aaggggaagc aatagctgaa ggtaaaggga aaacgaaaaa agaatcagaa caacgtgctg
    20581 ctgaaagtgc ctataagcaa ttaaaacaaa ttaaatagaa atttatatga tacgacatat
    20641 aggtgttcga tttaggattt aaggaagaat ttttaatatt ttatggaaca acttcgaaat
    20701 ttaatataca aatactaaaa tatcgtatac atgtgttctt taaaattgtg ataaggagtt
    20761 taggatggtt tatttaaaat caatagatgc cattggattt aagtcttttg cagatcaaac
    20821 caatgttcaa ttcgataaag gtgtaactgc aattgttggt ccaaatggaa gcggtaaaag
    20881 taatattaca gatgctatta aatgggtgtt gggtgaacaa tcggctaaat cattacgtgg
    20941 ctcaaaaatg gaagatatta tcttctcagg tgcagaacat cgcaaagctc aaaattatgc
    21001 tgaagtacag ttaagattag ataatcattc taaaaagctc agtgttgatg aaaacgaagt
    21061 tattgtaaca agaagattgt atcgaagtgg tgaaagtgag tactacataa ataatgaccg
    21121 tgcaagatta aaagatattg ccgatttatt tttagattct ggattgggaa aagaagcgta
    21181 tagcattatc tcgcaaggta gagttgatga aatactaaat gctaaaccaa ttgatagacg
    21241 tcaaattatt gaagaatcgg ctggtgtact taaatataaa aaacgtaagg ctgaatcatt
    21301 aaataaactt gaccaaacag aagataattt aacgagagta gaagacattt tatatgattt
    21361 ggaaggtcgc gtagaacctc taaaagagga ggcagctata gctaaagaat ataagacact
    21421 ttcacatcaa atgaaacata gtgacattgt agttacagtg cacgatattg atcaatatac
    21481 aaatgacaat agacaattag atcaacgttt aaatgattta caaggccaac aagcaaataa
    21541 agaagctgac aagcaacgtt taagccaaca aattcaacaa tataaaggta aacgtcatca
    21601 acttgataat gatgttgaat cgcttaatta tcaattagta aaagctacgg aagcctttga
    21661 aaaatatacg ggacaattaa atgttttgga agaacgtaag aaaaatcaat ctgaaacaaa
    21721 tgcacgatat gaagaagaac aagaaaattt aatggagctt ttagaaaata tatcaaatga
    21781 gatttctgaa gctcaagata cttataagtc tctgaaaagt aaacaaaaag aactcaatgc
    21841 tgtcattcgt gaacttgaag aacaactata tgtttcagac gaagcacatg atgaaaaatt
    21901 ggaagaaatt aaaaacgaat actatacatt aatgtcagag caatcagatg ttaacaatga
    21961 tattcgtttt ttaaagcata ctatagaaga gaatgaggct aaaaaatcaa gactagattc
    22021 tcgattagtt gaagtttttg agcaattgaa agatattcag ggtcaaataa aaacgacaaa
    22081 aaaagaatat caacagacca acaaagaact ttctgctgta gataaagaaa ttaaaaatat
    22141 agaaaaagac ctcactgata caaaaaaagc acaaaatgaa tacgaagaga aattgtatca
    22201 agcatatcga tataccgaaa aaatgaaaac acgtattgat agtttggcaa cgcaagagga
    22261 agaatatact tattttttca atggcgtcaa acatattttg aaagctaaaa ataaagaatt
    22321 aaagggtatt tatggtgcag ttgcggaaat tattgatgtg ccatctaaat taactcaggc
    22381 aattgaaaca gcattaggtg cttcattaca acatgtcatt gtagattcag aaaaagatgg
    22441 acgccaggct attcaatttt taaaagaacg taatttaggt cgtgcgacgt ttttaccatt
    22501 aaatgttata cagagtagag tggtagcgac tgatattaaa tctattgcta aagaggcaaa
    22561 cggatttatt agtatcgctt cggaagcagt taaagtagca ccagaatatc aaaatattat
    22621 cgggaattta ttaggtaata cgattatcgt tgatcattta aagcatgcaa atgaattggc
    22681 acgtgcgatt aaatatcgaa ctcgtattgt tactttggaa ggtgatattg taaatcctgg
    22741 tggttctatg actggtggtg gcgctcgtaa gtcaaaaagt attctgtctc aaaaagacga
    22801 gttgacaaca atgagacacc aattagaaga ttacttgcgt caaacagaat catttgaaca
    22861 acaatttaaa gagttgaaga taaaaagtga tcaattaagt gaactgtatt ttgaaaaaag
    22921 tcaaaagcat aatacactta aagagcaagt gcatcatttt gaaatggagc tcgatagatt
    22981 aactacacaa gaaacacaaa taaaaaatga tcatgaagaa ttcgaatttg aaaaaaatga
    23041 tggttatacg agtgacaaaa gtcgacaaac tttgagtgaa aaagaaactt atctagaaag
    23101 tattaaagca tctttaaaac gactagaaga tgaaattgaa cgctacacaa aactttctaa
    23161 agaaggtaag gaaagcgtta ctaaaacaca acaaacctta catcagaaac aatctgatct
    23221 tgctgtggtt aaagagcgta ttaaaacaca acaacagaca atagatcgat taaataatca
    23281 aaatcaacaa actaaacatc aattaaaaga tgttaaagaa aaaattgcat tctttaattc
    23341 ggatgaagtg atgggcgaac aagcttttca aaatattaaa gatcaaatta atggtcaaca
    23401 agaaacgaga acacgcttat cagatgaatt agataaattg aaacaacaac gtattgagtt
    23461 gaatgaacaa atcgatgcgc aagaagctaa actacaagtt tgtcaccaag atattttagc
    23521 tatcgaaaat cactaccaag atattaaagc tgaacaatca aagctagatg tattaattca
    23581 tcatgcgata gatcatttaa atgatgaata tcaattgact gttgaacgtg cgaaatctga
    23641 atatacgagt gatgaatcga ttgacgcatt acgtaaaaaa gttaagttaa tgaagatgtc
    23701 gattgatgaa ctaggtcctg taaacttaaa tgcaattgaa caatttgaag agttaaatga
    23761 acgttataca tttttaagtg aacaacgtac agatcttcgt aaagctaaag aaacattaga
    23821 gcaaattata agtgaaatgg atcaagaggt tactgaaaga tttaaagaaa ctttccatgc
    23881 tattcaagga cattttacag ctgtgttcaa acaattgttt ggtggaggcg atgcagaatt
    23941 gcaattaact gaagccgatt atttaacagc tggtattgat attgtggtac aaccaccggg
    24001 taaaaagttg caacatttat cgttactgag tggtggtgag cgtgcattaa ctgctattgc
    24061 tttactattt gcaattttaa aagtaagatc tgcacctttt gttatattag atgaggttga
    24121 agctgcacta gatgaagcaa atgttattag atacgcaaaa tatttaaatg agttatcaga
    24181 cgaaacacaa ttcattgtta ttacacaccg taaaggaaca atggaatttg cagataggtt
    24241 atacggtgta acaatgcaag aatcaggtgt tactaaactt gtgagtgtga atttaaatac
    24301 aatagatgat gtgttgaagg aggagcaata atgagctttt ttaaacgctt aaaagataag
    24361 tttgcaacaa ataaagaaaa tgaagaagtt aaatccttaa cagaagaaca aggtcaagac
    24421 aaattagaag atacacattc tgaaggttca acgcaggacg caaatgattt agcagaaaat
    24481 gctgaagtga aaaagaagcc acgcaagttg agtgaagcgg attttgatga cgatggctta
    24541 atatcaattg aagattttga agaaattgaa gctcaaaaaa tgggtgctaa atttaaagca
    24601 ggactcgaaa aatctcgtca aaatttccaa gaacaattaa ataatttgat agcgagatat
    24661 cgtaaagtag atgaagactt ttttgaagct ttagaagaaa tgttaatcac tgcagacgtc
    24721 ggttttaata cagtgatgac gttaactgaa gaattacgta tggaagcaca acgacgtaat
    24781 attcaagata ctgaagattt gcgtgaagtc attgttgaaa aaatcgtaga gatttaccat
    24841 caagaagatg ataattcaga agctatgaac ttagaagatg gtcgtttaaa tgtcatttta
    24901 atggttggtg tgaatggtgt tggtaaaaca acaacaattg gaaaattagc ttaccgatat
    24961 aaaatggaag gtaaaaaagt aatgttagct gcgggcgata cttttagagc gggtgctatt
    25021 gatcaattga aagtttgggg cgaacgtgtt ggtgtagacg taattagcca aagtgaaggt
    25081 tctgatccag ctgctgttat gtatgatgcg attaatgccg ctaaaaacaa aggtgttgat
    25141 attttaatct gtgataccgc tggacgttta caaaataaaa caaatctaat gcaagaatta
    25201 gaaaaagtta agcgtgtaat taatcgagca gtgccagatg cgcctcatga agcattacta
    25261 tgtttagatg ctacaactgg tcagaatgcg ttgtcacaag ctagaaactt taaagaagta
    25321 acaaatgtta caggtattgt attaacgaaa ttagatggta cagccaaagg tggtatcgta
    25381 ttagccattc gtaatgaatt gcacatccca gttaaatatg taggtttagg tgagcaatta
    25441 gatgacttac aaccatttaa ccctgaaagt tatgtctacg gcttattcgc tgatatgatt
    25501 gaacaaaatg aagaaataac aacagttgaa aatgatcaaa ttgtaacaga agaaaaggac
    25561 gataatcatg ggtcaaaatg atttagttaa aacgttacga atgaattatt tgtttgattt
    25621 ttatcaatcc ttattgacga ataaacaacg taattatttg gaattatttt atcttgaaga
    25681 ttattcttta agtgaaatcg cagatacttt taatgtgagt agacaagcag tttatgataa
    25741 tataagaaga actggcgatt tagttgaaga ttatgaaaag aaattggaat tataccagaa
    25801 atttgagcaa cgccgagaaa tatatgatga aatgaaacaa catttaagta atccagaaca
    25861 aatacaacgt tatattcaac aattagaaga cttagaatag atataaattg agggaggaca
    25921 tcgatatggc atttgaaggg ttatcagaac gcttgcaagc gacgatgcaa aaaatgcgtg
    25981 gtaagggtaa acttactgaa gctgatataa agataatgat gcgtgaagta agattagcgt
    26041 tacttgaggc tgacgtaaac tttaaagtgg taaaagaatt tattaaaaca gtatcagaac
    26101 gcgcattagg ttccgatgta atgcaatcat taacaccagg gcaacaagtt attaaaatag
    26161 ttcaagatga attaacgcag ttgatgggtg gagaaaatac gtcgattaat atgtcaaata
    26221 aaccacctac tgttgttatg atggttggtt tacaaggtgc tggtaaaaca acaactgcag
    26281 gtaaattagc attattgatg cgtaaaaaat acaacaaaaa acctatgtta gttgcagcag
    26341 atatttatcg tccagcagcg ataaatcaat tacaaacagt agggaaacaa attgatattc
    26401 ctgtatacag tgaaggagat caagtaaagc cacaacaaat tgtaactaat gcattaaaac
    26461 atgctaaaga agaacattta gactttgtaa tcattgatac agcaggtcga ttacacatcg
    26521 atgaagcatt gatgaacgaa ttaaaagaag taaaagacat tgctaaacca aacgaaatta
    26581 tgttagttgt cgattcaatg acgggtcaag atgctgtcaa tgttgcagaa tcttttgacg
    26641 atcaacttga tgtcacaggt gttaccttaa ctaaattaga tggtgataca cgtggtggtg
    26701 cagctttatc tattcgttcg gtgacacaaa aaccaattaa atttgttggt atgagtgaaa
    26761 agttagatgg tttagagcta ttccatcctg aacgtatggc atcacgtatt ttaggtatgg
    26821 gtgatgtgtt aagtttaatt gaaaaagcgc aacaagatgt ggatcaagaa aaagcaaaag
    26881 atttagagaa aaagatgcgt gagtcatcgt ttactttaga tgatttttta gaacaacttg
    26941 atcaggtgaa aaatctagga ccactggatg atattatgaa aatgattcca ggtatgaata
    27001 aaatgaaagg gctagataag cttaatatga gtgaaaagca aattgatcat attaaagcga
    27061 ttatccagtc aatgacgccg gctgaaagaa acaatccaga cacattgaat gtatcacgta
    27121 aaaagcgtat tgctaaaggg tctggtcgtt cattacaaga agtcaatcgt ttgatgaaac
    27181 aatttaacga tatgaagaaa atgatgaaac agttcactgg tggcggtaaa ggtaaaaaag
    27241 gtaaacgcaa tcaaatgcaa aatatgttaa aaggtatgaa tttaccgttt taatgtttaa
    27301 tataacacgc tgatttttgt tgcaatgaga tgtgcatccc gaaagttata ctaaagaata
    27361 taactttatg gggtgcatat ttttagatac acaataataa ttaaaaactg aaattgctga
    27421 tttgctagaa catgaggtta ttgttgctaa tattaataag ttatattggg ggaacgtgtg
    27481 cggtagaagt ggtaccattc atgtccttac gagataaatt aacttaacac gtaggtggtg
    27541 aaatttacta tgaattttta taacctatta acagtctaat ttagtaatca catagttatt
    27601 attgtaagaa aaaggttcaa taattttcct ggtaaagaaa aaactcttta caaacattca
    27661 tacacctgtt aatattattt cttgtagaaa ataaaaatta aaacatgact taaaggagat
    27721 tttataaatg gcagttaaaa ttcgtttaac acgtttaggt tcaaaaagaa atccattcta
    27781 tcgtatcgta gtagcagatg ctcgttctcc acgtgacgga cgtatcatcg aacaaatcgg
    27841 tacttataac ccaacgagcg ctaatgctcc agaaattaaa gttgacgaag cgttagcttt
    27901 aaaatggtta aatgatggtg cgaaaccaac tgatacagtt cacaatatct tatcaaaaga
    27961 aggtattatg aaaaaatttg acgaacaaaa gaaagctaag taatttagcg taaaattgtt
    28021 ctaacaataa gaataactcg tttacactga cagttattac tcaatgatac gttgggaata
    28081 tcacatgtta gtaatataga acgtattggg taccataatg gtgccctttt tctttgaatt
    28141 attttcaatt aaaatagaag tgttcaaagc atagagttgg aggtaataga atgagagttg
    28201 aagttggtca aattgttaac acacatggta ttaaaggtga aattaaagta aaatccaatt
    28261 cagactttac agacgttcgt tttcaacccg gtcaagtgct gacagttgtg cataacaata
    28321 acgaccttga atatactgtt aagtcacata gagtgcataa agggcttcat atgcttacat
    28381 ttgaaggtat taataatatt aatgatattg agcatttaaa agggagttct atttatcaag
    28441 agcgtgatca tgaagatatc gtacttgagg aaaatgaatt ttattattca gatattatag
    28501 gatgtacagt ttttgatgat caagaaacac caataggtcg tgtaattaat atatttgaaa
    28561 caggtgcgaa tgatgtgtgg gtgattaaag gatctaaaga atatttgatt ccttatattg
    28621 ctgatgttgt aaaagaagtg gatgttgaaa ataaaaaaat tatcatcacg ccaatggaag
    28681 gattgttgga ttaatgaaaa ttgattattt aactttattt cctgaaatgt ttgatggtgt
    28741 tttaaatcat tcaattatga aacgtgccca agaaaacaat aaattacaaa tcaatacggt
    28801 taattttaga gattatgcaa ttaacaagca caaccaagta gatgattatc cgtatggtgg
    28861 cggacaaggt atggtgttaa agcctgaacc tgtttttaat gcgatggaag acttagatgt
    28921 cacagaacaa acacgcgtta ttttaatgtg tccacaaggc gagccatttt cacatcagaa
    28981 agctgttgaa ttaagcaagg ccgaccacat cgttttcata tgcggacatt atgaaggtta
    29041 cgatgaacgt atccgaacac atcttgtcac agatgaaata tcaatgggtg actatgtttt
    29101 aactggtgga gaattgccag cgatgaccat gactgatgct attgttagac tgattccagg
    29161 tgttttaggt aatgaacagt cacatcaaga cgattcattt tcagatgggt tattagagtt
    29221 tccgcaatat acacgtccgc gtgaatttaa gggtctaaca gttccagatg ttttattgtc
    29281 tggaaatcat gccaatattg atgcatggag acatgagcaa aagttgatcc gcacatataa
    29341 taaaagacct gacttaattg aaaaatatcc attaactaat gcagataagc aaatattaga
    29401 aagatataaa ataggattga aaaaaggtta gtattgtgct aacataatct gagtgtggaa
    29461 acactaaaat cactatgatc cgctgctata tatttgtcga ggcaagaaca taggttgaag
    29521 aggagaattt ataatgacaa atcacaaatt aatcgaagca gtaactaaat cacaattacg
    29581 tacagactta ccaagtttcc gtcctggtga tactttacgt gtacacgtac gtatcattga
    29641 gggtactcgt gagcgtatcc aagtattcga aggcgttgta attaaacgtc gtggcggtgg
    29701 cgtttctgaa acgtttacag ttcgtaaaat ttcatcaggt gttggcgtgg aacgtacatt
    29761 cccattacac acaccaaaaa ttgaaaaaat cgaagttaaa cgtcgtggta aagtacgtcg
    29821 tgctaaatta tattacttac gtagtttacg tggtaaagct gctagaatcc aagaaattcg
    29881 ttaatcagca tttaaacaaa gctatgtatg agtcaaattc gactcaaaca ataaagccat
    29941 ctaggtcact tttatggagt gatatagatg gctttttttg ctgtttagtt aatgtaaagt
    30001 tgataatgta attgtttatt ccttctctat tcataacgtg tttgctcaaa atggttatat
    30061 aaacaaaaat ctactaagtg ccattgaaaa ggacttagta gatttattaa atatatgcgt
    30121 agcaacatta tttttgtctt gcccatctcg tgaaaataat actacatata atgccaaata
    30181 tagtaattgt tataagtaaa taatagtatg gtggggtata gctcaattga atctttgtta
    30241 tatttttagg tgctttaatg ccggtcataa caccatttac ttgttcaact ttaagacttt
    30301 gatcacctga tgtcgctttc ataccttgat tatatgctgt tggcaaaaca atatacccag
    30361 atgaattttt atttttagta atagtataac catgctttgt cttactaact ttgacagctt
    30421 ctaatgaatt tgaagcgtct ttaagcgtgg tataatcttc gccgtatatc ccttttaaat
    30481 ttactcgata cttaccttta ggcaatgata atctaattct atctggagct ttaatgcgta
    30541 tcgttacggg tgttacaacg cgtcgatatt tataagtgag tttatttctt tcttgtgtat
    30601 attcattcac tttaacatca tgagctttat ccggcgaaag taattctaaa tccatttcaa
    30661 aatacaaatc tttaaattga ttagaaactg attttggcaa ctgtacagtt agaccaccat
    30721 tattttgttt aacttgtaat aaatgttttg taggagattg ccaggctgca ctatttaatt
    30781 taattgttga atctgatagt aaatttttat tggctttaaa atgtgtatta acatctttaa
    30841 tattgttaga aacaatccct tgcaacattg cttgttcttt atctaatgga gattttaatt
    30901 ctttattgga aaagacctta tttgtaatat gtgcacttgg ataatggatg gtatttttag
    30961 aatgaatcca acgaacttta ttgtctttgt gttcagactt aattttaaat ccatatggta
    31021 agttgtcatc atgattcact ctaattcgat cattaacatt ccaaagtgat agtaaatttt
    31081 gacgattgcc aagtaatcta taagtgctgt ttttatcgat tggcatatta atttggagtg
    31141 tcttgtcata atattttaaa atgtctccat taaaaatact agaatataat gaaatgccat
    31201 tataatgata tataaatggt gaatttaatg catagtctga catataatca atgcgattaa
    31261 atgagcctgt tgcattttga tttatctttt ttataagctg gtttacatag ttactatggt
    31321 aatcatgttg tttcaacgtt gataatgatt gttgataagg tttgattgcc atgtttttgt
    31381 tgttatctaa aatgacgatt tgttgaatca taacgattaa tactaatatt gcaacggtta
    31441 attttttata acgccataaa ctaaatttta aaataacggc aagcactatt agcaggataa
    31501 tacctactat aagtgcaagt gggtgtgtcg gtgatagtaa tacataaagt attgcgatga
    31561 tgcatacggg tattgttctg attaaataat atttcatatt taatgttgat aaatgttgaa
    31621 taaacaatcc gcaaagagca cttgatgata gtgctaagat atacacccaa cgcctttctg
    31681 gaaatgaaaa accattaaaa gcactgtcga aatactgtga taatgaacct ataaataata
    31741 tccatgttac tattgcgaaa agtctataaa agtaaaaacg atacagtttg aatgacaata
    31801 atgcaacgat agtaagaatt gaaatcgtaa tataaaatcc atcgctaaag aaaaaataat
    31861 gataatcaag tggtgtcaaa aacggtatat caacattggg attttgcttt ctgtcatttt
    31921 ccaaaaacgc agaaatgcca gtgaataaac caaatacact tgataacaca ctcaaaactg
    31981 tagcagatat gacgcaaatt aatttttgtg ttctagagac aatgtcatat ttgtaagtga
    32041 aaatgagtcg atataaatag tagcaaccta taataatagc ttgataataa ctgaaataaa
    32101 aattgctaaa tagtgttaag gctatcgcaa caatgaaaat accgattttg cgttgttgaa
    32161 aatatctttc caaaccaaga atcgataatg gcaataaata taataaattt ccataaaatg
    32221 accaagtaaa attaaagtat ataacgacag ttgacatgcc gtataaaatc gtagcgatca
    32281 tatttgctga gcgtttaaag tgtaatattt taaataagta gaaggtcacg acaaatgtta
    32341 tgatagctcg tatcatagcc ataataagtt ggtttgtcgg ccaaaaatgt attgtcgtcg
    32401 gattaaatat accaaccgtt tctcctattt taatgaatag aaaatttagc cacattaaag
    32461 gtgacagcga ataataatat gatagtcctt tcatataatc gccacctaat ccaaacgatg
    32521 catcatataa actagaaaaa ctacgtagat gttcatacaa atacatttga aatggcatca
    32581 tttgacggaa tccatctcca gccccgctaa aaacagtacc attcacaata taatcataga
    32641 tatgagtaga aaataaaata agcgttaata ttacactaat gaaagttata acaaagaatt
    32701 gtttgacgtt tgaatttagc cactttttta acacaacatt atcctcaact ttcaaattta
    32761 aaattaagtt taactgaaac taaagttaat gaggttcttg ataggtaaag acgaagatga
    32821 ctgtggaaca gataccttat catagttact taaactttgg atcattttca gtttatcatt
    32881 aaacaaatat attgaataat aaaaatgtca tactgataaa gatgaatgtc acttaataag
    32941 taacttagat ttaacaaatg atgattttta attgtagaaa acttgaaata atcacttata
    33001 cctaaatcta aagcattgtt aagaagtgtg acaatgttaa aataaatata gttgaattaa
    33061 tgaatttgtt ctataattaa caggttatag attttaataa tgagaaaaga attgacgaaa
    33121 gtaaggtgaa ttgaatggtt attcaatggt atccaggaca tatggcgaaa gccaaaagag
    33181 aagtaagtga acaattaaaa aaagtagatg tagtgtttga actagtagat gcaagaattc
    33241 catatagttc aagaaaccct atgatagatg aagttattaa ccaaaaacca cgtgttgtta
    33301 tattaaataa aaaagatatg tctaatttaa atgagatgtc aaaatgggaa caatttttta
    33361 ttgataaagg atactatcct gtatcagtgg atgctaagca cggtaaaaat ttaaagaaag
    33421 tggaagctgc agcaattaag gcgactgctg aaaaatttga acgcgaaaaa gcgaaaggac
    33481 ttaaacctag agcgataaga gcaatgatcg ttggaattcc aaatgttggt aaatccacat
    33541 taataaataa actggcaaag cgtagtattg cgcagactgg taataaacca ggtgtgacca
    33601 aacaacaaca atggattaaa gttggtaatg cattacaact attagacaca ccagggatac
    33661 tttggcctaa atttgaagat gaagaagtcg gtaagaagtt gagtttaact ggtgcgataa
    33721 aagatagtat tgtgcactta gatgaagttg ccatctatgg attaaacttt ttaattcaaa
    33781 atgatttagc gcgattaaag tcacattata atattgaagt tcctgaagat gcagaaatca
    33841 tagcgtggtt tgatgcgata gggaaaaaac gtggcttaat tcgacgtggt aatgaaattg
    33901 attacgaagc agtcattgaa ctgattattt atgatattcg aaatgctaaa ataggaaatt
    33961 attgttttga tatttttaaa gatatgactg aggaattagc aaatgacgct aacaattaaa
    34021 gaagttacgc agttgattaa tgcggttaat acaatagaag aattagaaaa tcatgaatgc
    34081 tttttagatg agcgaaaagg tgttcaaaat gccatagcta ggcgcagaaa agcgttagaa
    34141 aaagaacaag ctttaaaaga aaagtatgtt gaaatgactt actttgaaaa tgaaatatta
    34201 aaagagcatc ctaatgctat tatttgtggg attgatgaag ttggaagagg acctttagca
    34261 ggtccagtcg ttgcatgcgc aacaatttta aattcaaatc acaattattt gggccttgat
    34321 gactcgaaaa aagtacctgt tacgaaacgt ctagaattaa atgaagcact aaaaaatgaa
    34381 gttactgctt ttgcatatgg gatcgcgaca gctgaagaaa tagatgaatt taatatttat
    34441 aaagctactc aaatcgccat gcagcgagct attgatggat tatcagtaca accaacgcat
    34501 ttattgatag acgcgatgac gcttgataat gcactgcctc aagtatcttt aatcaagggt
    34561 gatgcaagaa gtgtgtccat tgcagctgca agtatcatgg caaaggtttt tcgtgatgat
    34621 tatatgacac agttatctaa agattatcct gaatatggtt ttgaaaaaaa cgcgggttac
    34681 ggtaccaaac aacatttact agcaatcgat gatattggca ttatgaaaga gcatagaaaa
    34741 agctttgaac ctataaaatc gttactgtaa atcgtaaaat tcaattaaat gataaaaaag
    34801 tagcatttaa gtttacaata gcgcttacat tttttataat tgtaagggaa cttaacctaa
    34861 gtaacaggag gatggaagat gaatatccac gagtatcaag gtaaagaaat atttcgttca
    34921 atgggcgttg cagttccaga aggacgagta gcatttactg ctgaagaagc ggtggagaaa
    34981 gcaaaagaat taaattctga tgtttatgtt gtaaaagcac aaattcatgc tggaggtaga
    35041 ggtaaagcag gcggagtaaa aattgctaaa tctttatctg aggtagaaac atatgcaaaa
    35101 gaattattag ggaaaacttt ggtgacacat caaactggtc cagaaggtaa agaaattaag
    35161 cgtttatata tcgaagaagg ttgtgctatt caaaaagaat attacgttgg attcgttatt
    35221 gatcgtgcga ctgaccaagt aacattgatg gcgtctgaag aagggggcac tgagattgaa
    35281 gaagttgctg cgaagactcc tgaaaagatc ttcaaagaaa ctatcgatcc agtaatcgga
    35341 ctttcaccat tccaagcaag acgaattgcg tttaatatta atattcctaa agaatctgtt
    35401 aacaaagctg ctaaattctt attagcactt tataatgtat tcattgaaaa agattgttca
    35461 atcgtagaaa tcaacccatt agttacaaca gctgatggtg atgtattggc attagatgct
    35521 aaaattaatt ttgatgataa tgcattattc agacataaag atgttgtaga attacgtgat
    35581 ttagaagaag aagatccgaa agagattgaa gcgtctaaac atgatttatc atacattgca
    35641 ttagatggtg acatcggatg tatggttaat ggtgcaggtt tagccatggc aacaatggat
    35701 acgattaatc atttcggtgg aaacccagcc aatttcttag atgcaggcgg aagcgctact
    35761 agagaaaaag taactgaagc atttaaaatc attttaggtg atgaaaatgt taaaggtatt
    35821 tttgtaaaca ttttcggtgg cattatgaaa tgtgatgtta tcgcagaagg tatcgttgaa
    35881 gctgtaaaag aagtagattt aactttacca ctagttgtac gcctagaagg tacaaatgtt
    35941 gagttaggta aaaaaatctt aaaagactca ggattagcaa ttgaaccagc agcaacaatg
    36001 gctgaaggtg cacaaaaaat tgttaaacta gtcaaagaag cataaggaaa ggatgggagc
    36061 actaagatga gtgtatttat agataagaat actaaagtaa tggtacaagg tattacaggg
    36121 tctactgccc ttttccatac aaaacaaatg cttgattatg gtacgaaaat agtagcaggt
    36181 gtgacgcctg gtaaaggtgg tcaagttgtt gaaggcgttc ctgttttcaa cactgttgaa
    36241 gaagctaaaa atgaaactgg ggcaacggtt tcagtcattt acgttccagc accatttgct
    36301 gcagactcaa ttttagaagc agctgatgca gacttagata tggttatttg tatcactgaa
    36361 catattcctg tattagacat ggttaaagtt aaacgctact tacaaggtag aaaaacacgt
    36421 ttagttggtc cgaactgtcc aggtgtgatt acagcagatg aatgtaaaat tggtattatg
    36481 cctggctata ttcacaaaaa aggtcatgtt ggtgtagtat ctcgttcagg tacattaaca
    36541 tatgaagcag tgcaccaatt gactgaagaa ggtattggtc aaactacagc tgttggtatt
    36601 ggtggagacc cagtcaacgg aacaaacttt attgatgttt taaaagcatt caatgaagat
    36661 gacgaaacga aagcagttgt tatgattggt gaaatcggtg gtacggctga agaagaagca
    36721 gctgaatgga ttaaagcgaa tatgacaaaa ccagttgtag gctttatcgg tggacaaaca
    36781 gcacctcctg gaaaacgtat gggacatgct ggtgcaatca tttcaggtgg taaaggtact
    36841 gctgaagaga aaattaaaac attaaatagt tgtggtgtga aaacagcggc aacaccttca
    36901 gaaattggtt caacattaat tgaagctgct aaagaagcag gtatttatga atcattatta
    36961 actgttaata aataaagtta aaagatgata taaatggcta tagcccattc catactttat
    37021 aaaagtatcg gaaatgggct atttgtcgtg cgatgaattt tagtacactt ttcatatttt
    37081 ggaaagacaa gttaattatt ttacctccta caatagcttt ataaaacaaa gaaaatttta
    37141 attaaaatat atagcgcact atgttataat gtttgtatat tattgtaagg agtgtttcat
    37201 catgaataaa caacaaagta aagtacgcta ttcaattaga aaagttagta ttggaatttt
    37261 gtcaatttca ataggtatgt ttttggcatt gggtatgtcg aacaaagcat atgcagatga
    37321 aattgataaa tctaaagatt ttacaagagg gtatgagcaa aatgtattcg cgaaatcaga
    37381 gttaaatgct aataaaaata cgacaaaaga caaaataaaa aatgaaggtg ctgttaaaac
    37441 atcggacaca agtttaaagt tagacaacaa atcagcaatt tcaaacggaa atgaaattaa
    37501 tcaagatata aagatttcaa atactccgaa aaactcaagc caaggtaaca atctagttat
    37561 taataacaat gaacttacta aagaaattaa aattgcaaac ttggaagctc aaaattctaa
    37621 tcagaagaaa acgaataaag ttactaataa ttactttggt tactacagtt ttagagaagc
    37681 tccaaaaaca caaatctata ctgtaaaaaa aggagacaca cttagtgcta tagcattaaa
    37741 atacaaaact aca
//
LOCUS       DANJFZ010000014        37678 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.014, whole genome shotgun sequence.
ACCESSION   DANJFZ010000014 DANJFZ010000000
VERSION     DANJFZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.014"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(37..630)
                     /locus_tag="PWN45_000579"
     CDS             complement(37..630)
                     /locus_tag="PWN45_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0622368.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            694..1077
                     /locus_tag="PWN45_000580"
     CDS             694..1077
                     /locus_tag="PWN45_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDL0622369.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            1208..3613
                     /gene="mnhA1"
                     /locus_tag="PWN45_000581"
     CDS             1208..3613
                     /gene="mnhA1"
                     /locus_tag="PWN45_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDL0622370.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            3606..4034
                     /gene="mnhB1"
                     /locus_tag="PWN45_000582"
     CDS             3606..4034
                     /gene="mnhB1"
                     /locus_tag="PWN45_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDL0622371.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4034..4375
                     /gene="mnhC1"
                     /locus_tag="PWN45_000583"
     CDS             4034..4375
                     /gene="mnhC1"
                     /locus_tag="PWN45_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDL0622372.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            4368..5864
                     /gene="mnhD1"
                     /locus_tag="PWN45_000584"
     CDS             4368..5864
                     /gene="mnhD1"
                     /locus_tag="PWN45_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDL0622373.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            5866..6345
                     /gene="mnhE1"
                     /locus_tag="PWN45_000585"
     CDS             5866..6345
                     /gene="mnhE1"
                     /locus_tag="PWN45_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDL0622374.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            6345..6638
                     /gene="mnhF1"
                     /locus_tag="PWN45_000586"
     CDS             6345..6638
                     /gene="mnhF1"
                     /locus_tag="PWN45_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDL0622375.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            6616..6972
                     /gene="mnhG1"
                     /locus_tag="PWN45_000587"
     CDS             6616..6972
                     /gene="mnhG1"
                     /locus_tag="PWN45_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDL0622376.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            7221..8375
                     /locus_tag="PWN45_000588"
     CDS             7221..8375
                     /locus_tag="PWN45_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL0622377.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(8431..8805)
                     /locus_tag="PWN45_000589"
     CDS             complement(8431..8805)
                     /locus_tag="PWN45_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDL0622378.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(8824..10140)
                     /locus_tag="PWN45_000590"
     CDS             complement(8824..10140)
                     /locus_tag="PWN45_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL0622379.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(10551..12026)
                     /locus_tag="PWN45_000591"
     CDS             complement(10551..12026)
                     /locus_tag="PWN45_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDL0622380.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(12157..13365)
                     /locus_tag="PWN45_000592"
     CDS             complement(12157..13365)
                     /locus_tag="PWN45_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622381.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(13819..14178)
                     /locus_tag="PWN45_000593"
     CDS             complement(13819..14178)
                     /locus_tag="PWN45_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDL0622382.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(14191..14427)
                     /locus_tag="PWN45_000594"
     CDS             complement(14191..14427)
                     /locus_tag="PWN45_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDL0622383.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            14744..15808
                     /locus_tag="PWN45_000595"
     CDS             14744..15808
                     /locus_tag="PWN45_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622384.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(15867..16190)
                     /locus_tag="PWN45_000596"
     CDS             complement(15867..16190)
                     /locus_tag="PWN45_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDL0622385.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            16291..16533
                     /locus_tag="PWN45_000597"
     CDS             16291..16533
                     /locus_tag="PWN45_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDL0622386.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(16796..17971)
                     /gene="dltD"
                     /locus_tag="PWN45_000598"
     CDS             complement(16796..17971)
                     /gene="dltD"
                     /locus_tag="PWN45_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL0622387.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(17968..18204)
                     /gene="dltC"
                     /locus_tag="PWN45_000599"
     CDS             complement(17968..18204)
                     /gene="dltC"
                     /locus_tag="PWN45_000599"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDL0622388.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(18222..19436)
                     /gene="dltB"
                     /locus_tag="PWN45_000600"
     CDS             complement(18222..19436)
                     /gene="dltB"
                     /locus_tag="PWN45_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDL0622389.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(19433..20890)
                     /gene="dltA"
                     /locus_tag="PWN45_000601"
     CDS             complement(19433..20890)
                     /gene="dltA"
                     /locus_tag="PWN45_000601"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL0622390.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(20906..21058)
                     /locus_tag="PWN45_000602"
     CDS             complement(20906..21058)
                     /locus_tag="PWN45_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL0622391.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(21106..21216)
                     /locus_tag="PWN45_000603"
     CDS             complement(21106..21216)
                     /locus_tag="PWN45_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622392.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(21470..22429)
                     /locus_tag="PWN45_000604"
     CDS             complement(21470..22429)
                     /locus_tag="PWN45_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDL0622393.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(22462..23241)
                     /locus_tag="PWN45_000605"
     CDS             complement(22462..23241)
                     /locus_tag="PWN45_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL0622394.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(23241..23675)
                     /locus_tag="PWN45_000606"
     CDS             complement(23241..23675)
                     /locus_tag="PWN45_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDL0622395.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            23773..24039
                     /locus_tag="PWN45_000607"
     CDS             23773..24039
                     /locus_tag="PWN45_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDL0622396.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(24118..24501)
                     /locus_tag="PWN45_000608"
     CDS             complement(24118..24501)
                     /locus_tag="PWN45_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL0622397.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(24631..25548)
                     /gene="lipA"
                     /locus_tag="PWN45_000609"
     CDS             complement(24631..25548)
                     /gene="lipA"
                     /locus_tag="PWN45_000609"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL0622398.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(25632..26951)
                     /locus_tag="PWN45_000610"
     CDS             complement(25632..26951)
                     /locus_tag="PWN45_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL0622399.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(26978..27805)
                     /locus_tag="PWN45_000611"
     CDS             complement(26978..27805)
                     /locus_tag="PWN45_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL0622400.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(27818..28666)
                     /locus_tag="PWN45_000612"
     CDS             complement(27818..28666)
                     /locus_tag="PWN45_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL0622401.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(28714..28812)
                     /locus_tag="PWN45_000613"
     CDS             complement(28714..28812)
                     /locus_tag="PWN45_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622402.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            complement(28879..29946)
                     /locus_tag="PWN45_000614"
     CDS             complement(28879..29946)
                     /locus_tag="PWN45_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL0622403.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(29960..31000)
                     /locus_tag="PWN45_000615"
     CDS             complement(29960..31000)
                     /locus_tag="PWN45_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0622404.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            31365..31679
                     /locus_tag="PWN45_000616"
     CDS             31365..31679
                     /locus_tag="PWN45_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622405.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(31685..31822)
                     /locus_tag="PWN45_000617"
     CDS             complement(31685..31822)
                     /locus_tag="PWN45_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL0622406.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(32009..33406)
                     /gene="sufB"
                     /locus_tag="PWN45_000618"
     CDS             complement(32009..33406)
                     /gene="sufB"
                     /locus_tag="PWN45_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL0622407.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(33557..34021)
                     /locus_tag="PWN45_000619"
     CDS             complement(33557..34021)
                     /locus_tag="PWN45_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL0622408.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(34011..35261)
                     /locus_tag="PWN45_000620"
     CDS             complement(34011..35261)
                     /locus_tag="PWN45_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0622409.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(35367..36674)
                     /gene="sufD"
                     /locus_tag="PWN45_000621"
     CDS             complement(35367..36674)
                     /gene="sufD"
                     /locus_tag="PWN45_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL0622410.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(36772..37533)
                     /gene="sufC"
                     /locus_tag="PWN45_000622"
     CDS             complement(36772..37533)
                     /gene="sufC"
                     /locus_tag="PWN45_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL0622411.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT        11852 a         6813 c         5608 g        13405 t
ORIGIN      
        1 gagtttaaaa tgttggtagt taattatgtt tagatattat tcttcaacat caatagattc
       61 aattacaaca tcatgaagtg gtttatcttg tggtcccact tttgtatttg caatatcttc
      121 taatgtagtt tcaccatcaa tgatttgacc gaatactgta tgtttttgat ctaaccatgg
      181 tgtaccaccc ttttcgccat atgcatcaac gattggttga ggccagccac catctgcaag
      241 ttgacttaac atattttgag gtacttcttt catttgaaca atgaaaaatt gtgaaccatt
      301 agtattaggt cctgagttag ccattgataa tgcgccatat aagttaaatg catttaatga
      361 aaattcatct tcaaaagcac cgccataaat actttcgcca cccataccag tagctgttgg
      421 atcgccacct tgaatcatga agtcattaat gacacggtgg aatgtgattc catcataata
      481 accatttttt gcatgtgtca caaaattttc aactgttttt ggtgcaatat ttggaaataa
      541 tttgaatgtc atgtcacctt tatttgtgtg cataaccact ttgatttcac cttgttgtac
      601 ttctttgttt aactgtggat agttagccat ttatattctc cattcatgtt atgatacata
      661 tatattaaca tacttttaag ggaggaaaat agcatgaagc tatatcgatt ttcacacaaa
      721 acaggtagct atggtgtcac gattgttgaa gaaaatggag acgatgtcct tgttaaagtt
      781 gaacaagtta tcaaacaccc gaaacaaggt gatttacata accctactga aactgaaggt
      841 gtatttttcc atgaccgcaa agcattaagc cattttgaaa aacgctatac gaaacgttca
      901 caattacgcg acttcaatgt tgatgtattg cgctatgaag attcacttca acaagcaatt
      961 acagatttag aaactaaact taaagctcag caaacaaaac atgctgaaat gtctttagct
     1021 agtttagcac gcttaaaaga agattattca cttcaataca aagaaaattt taaataaaaa
     1081 atgacgtgtt tcttcttatt taaatacttc tgaaaataaa gcacaacaag gagtgtgtac
     1141 gtttttcaga actatatcat tcatgtataa tgaataacat aaatattttt taaaggagga
     1201 caacatagtg agtttgttac atattgcggt gatattaccg ttaatattcg cactcattat
     1261 tcctatcctg tatagatttt ttaaacgtat acatttagga tggttcgtat tacctgtccc
     1321 tatcgtcata tttatttata tgcttacatt aattaaaacg acgatgtcag gtaatactgt
     1381 aatgaaaacc ttaaattgga tgcctcattt tggtatgaac tttgatttat atcttgatgg
     1441 cttaggttta ctctttagtt tgcttatttc aggaatcggc agtttagtag tattatattc
     1501 aatcggctac ttaagtaagt cagaacaact agggaatttt tattgttatt tattactatt
     1561 tatgggtgca atgttaggtg tcgtactttc agataatgtt attattctat atttattctg
     1621 ggagctaacg tcattttcta gttttttact tatttcattc tggcgcgaac gacaagcatc
     1681 tatatatggt gctcaaaaat cgctcataat cacagtattt ggtggtctat cattattagg
     1741 tggtatcatc ttactcgcta taccaacaca atcatttagc attcagtaca tgattcaaca
     1801 tgcttctgaa attcaaaata gcccattctt catttttgcg atgattttaa tcatgattgg
     1861 tgcttttaca aaatctgctc agtttccatt ttatatttgg ctaccagatg caatggaagc
     1921 acctacacca gtcagtgctt atcttcattc agcaacaatg gttaaagcag ggttatattt
     1981 aatcgctcga atgacaccaa tcttcgcagc atcgcaaggt tgggtatgga ctgtcacact
     2041 cgttggttta attacattat tctgggcttc gctaaacgca acaaaacaac aagacttgaa
     2101 aggtatactg gcattttcaa cagtatccca attaggtatg attatggcga tgcttggaat
     2161 cggtgccatt agttatcact atcaaggcga tgatagcaaa atatatgctg ctgcatttac
     2221 ggctgcaata ttccatttaa tcaaccatgc aacatttaaa ggtgccctat ttatgattac
     2281 aggtgcagtg gatcattcta ctggtacacg tgatgttaag aaattaggtg gcttactaac
     2341 aattatgcct atttcattta caatcactgt tattacagca ttaagtatgg ctggtgtacc
     2401 accgttcaat ggtttcttat caaaagaatc atttttagaa acaacattta cagcaagcca
     2461 agcaaattta ttcagtgttg atacattagg ctacctattc ccaatcattg gtatagtggg
     2521 cagtgtcttc acatttgtat attcaattaa atttataatg catatatttt tcggacaata
     2581 taaacctgaa caattaccta aaaaagcaca cgaagtatca atattaatgc ttttatcacc
     2641 agctatttta gctacattag taattgtatt tggtttattc cctggcattt taactaattc
     2701 gattattgaa ccagctacat cttcgattaa tcatacggta attgatgatg ttgaattcca
     2761 tatgttccat ggattaacgc ctgccttttt atcaacatta gtgatttata ttttaggtat
     2821 cttactaatt gtgacattta gttattgggt gaaattgttg caacgacaac ctggtaaact
     2881 aacattcaat tattggtaca acagaagtgc aaatgttatt ccaaactact ctgaaaagat
     2941 gacgaatagc tatgttaccg attattcacg taataattta gttatcattt ttggtgcatt
     3001 aattttatta acatttgtca ctatattctc agtacctttc aacattaatt ttaaagacgt
     3061 tagtccgatt agaattttcg aagtttgtat cgtaatttta ttattaagtg ctgccttttt
     3121 aattcttttc gctaaatcaa gattatttag catcattatg ttaagtgcag ttggatatgc
     3181 tgtgtctgta ttgtttatat tctttaaagc acccgactta gcattgacac agtttgtcgt
     3241 tgaatctatt tcaacagcat tgttcttatt atgcttctac cacttaccaa atttaaatcg
     3301 ttataatgaa aagcgctcat tccagcttac taatgcttta attgctggtg gtgttggttt
     3361 gtccgtcatt attatcggtt taattgcata cggaaataga cattttgaat cgatatctaa
     3421 gttttatcaa gagcacgttt atgatttagc tcacggtaaa aacatggtaa acgtcatact
     3481 tgttgatttc cgtggtatgg atactttatt cgagtcatct gtgcttggta tcgcaggatt
     3541 agctgtatat acaatgatta aattacgtaa aaagcgtcaa acgcaaggta acgaggtgaa
     3601 aaatcatgaa tagacaacaa aatgatctaa ttttgcaatt tgctgcagtt attatctttt
     3661 tcatggtaat ggtctttggt ttttcacttt tcttagcagg acattataca cctggtggcg
     3721 gtttcgtcgg cggattatta ttcgcaagtt cacttgttat cattacgatt gcatttgata
     3781 tagaaacaat gcgtaaaatt ttcccattag attttaaaat cttgataggt atcggattag
     3841 ttttttgtat tgcgacgcct atcgcaagtt ggtttttagg caaaaatttc tttacgcatg
     3901 tcacatttga tataccgtta tttatattag aacctgttca tatgacaaca gcagtattct
     3961 ttgatttcgg tgttttatgt gcagtagttg gtactgtcat gacaattatt atttcgattg
     4021 gagagaacga atagtggaaa ttattatgat ttttgttagt ggtattctca cagcaattag
     4081 tgtctatctc gttttgtcta aaagtctgat acgaattgtt atgggaacta cactattaac
     4141 acatgcagca aatttatttt taataactat gggcggactt aaacatggta ctgttccaat
     4201 ttatgaagcg aacgtaaaaa gctatgttga tcctatcccg caagcactta ttttaacagc
     4261 aatcgttatc gcctttgcga caacagcctt tttcttagta ttagcattta gaacatataa
     4321 agaattaggc acagataacg ttgagagtat gaaaggagtt ccagaagatg attgaaagta
     4381 atatgcttgt gttaacgcta gttattccag taattacagc catattactt gtctttattg
     4441 gaaagcgtcc tattataaag cgttatgtcg cactaggcgg tacattatta accttggtcg
     4501 cggcaattat caatttagca aatgttgtta aacatggacc aatacgcgta gagctaggtt
     4561 catggaaagc accatacagt attgtctttg tattagacat attcagtgcc ctactaataa
     4621 ttactagtat tattattaca gctattgtga ttttatattc ataccaaaca attggtattg
     4681 aacgtgaaag atattattat tatttttcag tactatttat gttaataggt atcattggtg
     4741 cattcacaac tggtgacatt ttcaacctat tcgtatattt cgaagtattt ttaatgtcat
     4801 cttatttctt attagtaatt ggttctacaa aaattcaact acaagaaaca ataaaatatg
     4861 ttcttgttaa cgttgtatcg tcatccttct tcgtaatggg tgtagcgatt ttatattcag
     4921 tagtaggaac attgaatcta gcagatataa gcaataaatt agctaacctt tcagcgcatg
     4981 acagtggctt agtcaatatc gttttcatac tatttatctt tgtttttgct acaaaagctg
     5041 gcgtattccc aatgtttgtt tggttaccta gtgcatacta tgcaccacca attccaatca
     5101 ttgcattctt tggtgctttg ttaacaaaag ttggtgttta tgcaatcgct agaacattga
     5161 gcctattctt tagtgataat gtaagctttt cacattatgt cattttattc ttagcactat
     5221 taacgatcat ctttggttgt gtcggtgctg ttgcatatgc caacattaaa aaaatcatcc
     5281 tatacaatgt catgattgct gtaggtgtga tcttagtcgg cgttgcaatg atgacagagt
     5341 caggtatgat tggggctatt tactatacat tgcacgacat gttagtaaaa cttgcattat
     5401 tcttgcttat cggaattatg attaaaatta caggcactgc tgatttaaga caatttggtg
     5461 gcttaattaa acgatatcct gtacttggtt ggtcattctt tatcgcagca ttaagtttag
     5521 ctggcattcc cccactcagt ggcttttatg gaaaattttt tattgtccaa tcaacatttg
     5581 aaagaggatt ttatttaagt ggtgtcatcg tcttattatc aagtttagtc gtattatatt
     5641 ctgtcattcg catattccta caaggctttt tcggtcaacc aaaaggatat gatcttaata
     5701 acaaagtcga cgtcaaatat ttaactacga ttgcaatcgt tgcagttgtg ataactgttt
     5761 tatatggatt gtcagcagat tacttatatc caatggtaaa agctggtgca gaaacattct
     5821 acaatccatc aacctacgtt aaagctgtat taggaggtaa ataacatggc tgtacaatta
     5881 gtattaaact ttattatcgc agtgttttgg ttgtttgtga caaatagtta cacaacaaat
     5941 aactttgtgt taggtttcat ctttggccta gttcttgttt acctcttaca cagagtgtta
     6001 ccaggtagat tttatgtcat cacgctatat cgtattatta aattagttat tattttctta
     6061 atcgaactga ttaaagctaa ttttgatgtt ttgaaaatta ttattaaacc ttcgattaaa
     6121 aatgaaccag gattctttgt ttatcacaca gatttaaaaa aagattggca aattgtcttg
     6181 ctgtctaatt taattacatt aacacctggt actgtagtgc ttggtgtaag tgacgatcgt
     6241 acaaaaattt atattcatgc catagatttt tcaactaaag agcaagaagt agaaagtatt
     6301 aaaacatcat tagagaaaat tgtaagagag gtgggcgaaa tataatgaat cataatgtta
     6361 ttatcgttat tgcattaatc atagttgtca tttctatgtt agctatgctc attcgcgttg
     6421 tgctaggccc atcacttgcc gatcgtgttg tcgcattaga tgcgattggt cttcaattaa
     6481 tggcagttat agcattattc agtattttat taaatattaa atacatgatt gtcgttatta
     6541 tgatgattgg tatattagct tttttaggta ctgcagtatt ctctaaattt atggacaaag
     6601 gtaaggtgat tgaacatgat caaaatcata ctgattagtc ttgcacttat ctttgttatc
     6661 atcggtgctt taattagcgc cctagcagct ataggattat tgagacttga agatgtatat
     6721 tcacgtgcac atgctgccgg aaaagcatca acattaggtg caatgtcatt actatttggt
     6781 acgtttttat attttattgc tactcaaggt tttgtaaata tgcaattaat cgttgcgatt
     6841 atctttgtat taattacagg tcctctttca agtcatatga ttatgaaagc agcatataat
     6901 attaaaacgc cttatactaa aaagactaaa gtcgatgaaa tttcggaaga cttaaaagac
     6961 acaaaattgt agattatata acaaaatcat gaatataaat caacaacaaa cagcagtaag
     7021 atgattccaa attaggaatg attttactgc tgttttcttt tgacattgtt acctcttttt
     7081 caatgatttt ttctttgact acagattcgc cctatctaca tatatctctt taatttaatt
     7141 gcctttcatg tcgttatgta ttatgataat aataattata aatcgtaacg attacgtttt
     7201 aaaaagagag aggttttatt atgcattgga caattatcgg cggtggcata cagggaactg
     7261 caatcgcaca aaaactatta tcaagcggat taacaacaga ccgattaaca atcattgacc
     7321 cacacgaaac tttttgccaa aggtttaact catatacaaa tcgaatagaa atgccttatt
     7381 taagatcacc gattgtacat cacgtacatc cacaaccgtt ccatctaaaa caattcgcta
     7441 aacagcacca atatacaaat gctttttatg gtccttatca acgacctgaa ttgacaatgt
     7501 ttatggatca tattgcacat gcttctaaac aatatcaatt agaggattgc ttggttcaag
     7561 gtttagttca aactttagat aaacaagaag acaaatggca tatcaagtta gaagatggac
     7621 aaattatcac tacagattgc gtcgttattg caataggcag tacaaatatt ccgtttatgc
     7681 ctgacatttt aaaagacaaa cagaatgtaa atcatatctt cgagaaagaa cttgatcaag
     7741 tagtatatga taagaccgat catatcgttg gtagcggcat tactgctgca catcttgcac
     7801 ttaaattgtt aaatcatgat aacgataaaa agattcattt atggctaaat aaagatattg
     7861 aaatacatga ctttgatgct gatcctggtt ggttaggtcc gaaaaatatg tcttcatttt
     7921 taagtactaa aagcatgcct gaaagaaatg ccattgtaca acgcgaacgt cataaaggat
     7981 caatgcctca cgaactgtac ttacgcctta aaaaacatat taaaaatggt cgtataaatg
     8041 tgcataaaac acctatcact caaattagtg gtggtgtaat taacactgaa aatgattctg
     8101 ttccatatca acagattatg gttgcaactg gttttgaaca agattttatg tcacaaccac
     8161 ttattaagca attaatacaa aattatgatg cacctatcaa cgaatgtaat taccctgtta
     8221 tttccgaaaa attagaatgg ataccaaatc tatttgtcgc aggatgcttt gcagacttag
     8281 aattaggacc atttggtaga aatgttatgg gtggccgtaa agctgccgaa cgcattgaac
     8341 aagcatttct aaaactacaa caatatagcg cataaaaaaa ctgctagccg tagttatgaa
     8401 tatctcataa catttcagct agcagttctt ttattttaaa ggtttaatag caactgtacc
     8461 acgcataact gtaattaatt gttctttgtc attcttaatt tttatatccc atacatgtgt
     8521 cgacttacct cgatgaataa tttcagctgt cgcagtaaca cgaccatctt tagcagaatg
     8581 aatatggtta gcattcatct ctaaacctaa tggaataaat ttggttgtat caattaaatt
     8641 agcagatcct aatgaacatg ctgtttcacc taaagcaatc gaagccccac catgtaaata
     8701 tccaaatggt tgttttactt tatcagtaac aggcatagaa ataacaacta aaccatcttc
     8761 ctggtgatct attgacatct caaatgtctc taataaatga gtcatatttt cacctcttta
     8821 tgcttatagt accatagcag caatagtacc gaaaatcatt aaaggaatat tataaaacaa
     8881 gaagtttggt acacatgtat cacgtatatg atcatgttgg ccatcaacat ttaatcccgc
     8941 agttggtcct aatgttgaat cacttgcagg cgaacctgag tcacctaatg cactcgctgt
     9001 tccaatcaat gcgattaatg ccattgtatc tagtccaatt gacgctccaa aaggaatgaa
     9061 taatgatgcg ataataggaa ttgttgcaaa tgatgatcca atacctaaag tgacaattaa
     9121 acctatcaca tacatcatga taatgctaaa taatttatta tcaccagtaa tacttgttaa
     9181 agttttaact aattcatcta tatcaccagt agcattcatt acaccagcaa atccatttgc
     9241 tgttaaaata actacaccaa tataagccat aattttaata ccttcaacaa acttagcatc
     9301 taattcatac caattatatg cacgtgaaat aaagaatacg agtacccctg ccagtgcacc
     9361 aaaaatcatt gaatctgtaa atgtttgtac taaaaatgta gctagtattg ctacaattgt
     9421 tactattaag atatatggtt ttaactctgt aacattgtca ctatctgaaa ttttacgtgt
     9481 ttcatattca cgtggtttac gatatacata taaaccgata agtaagccaa caatataccc
     9541 cattgaagga ataagcattg ctttccaaat catattaaac tcaattgggt gatttgcctt
     9601 tgcaaagcca ctttgaataa tttgctggaa aatttgaccg aatccatatg gtaataacac
     9661 atacgggaaa cataaaccaa aaccgataat caaaccgatt aaacgtctat ctatttttaa
     9721 gtcattaaac agacttaaca atggtgggat gacaattgga atgaatgcaa tatgtacagg
     9781 aattaagttt tgactcatga tactcatagc taataatgca atgattattg tcactttgac
     9841 tttaactctt gaccatcgac tattttcagc gtgaattgca tttataattt ttcctactaa
     9901 atagtctgtg ataccactgt atgaaattaa tgctgcaaat ccacctaata aagcatagct
     9961 taatgctacc tcagcaccat cgactatatt tttcccaaat acatttataa ctttttcaat
    10021 gctcatgcct gaaattaagc caccaactag cgcactgata aataagctta taactacgtt
    10081 taatcgacat aaacatagca caatcattaa aattactgct attactactg catttatcat
    10141 atgacaacca tcctttatca ctctatcgaa ctaaagcgtt tatagactac atagtatcag
    10201 tagctttaac acctgtcaat agcaaactat accaataatc tttatttttt agttaattgt
    10261 ctttcttaaa ataattttag ctttcattaa attaaacaat tttacaagct tggaacacca
    10321 atcaaaatcc taagttctaa aatgcaatat tagtagtcgt tgactgaatg aacatatgct
    10381 tataatattt ttttgcaatg ctagtcaagt tgatttatgc tcacaaggat atgcgattta
    10441 tattttctta caacaatgaa aatgcctgat acaatgcgat cctttatact ttcaagagtt
    10501 aaattagaaa tgtatatcaa gacacaagtt gtatcaggca aataattaaa ttattgttgt
    10561 tttaaccatt gtactatcgt cggaatcata aatcctgttg gcccttttgg accattatat
    10621 gaagctttat tagtcgttgc tggacctgca atatcgaaat gaatgtgagg tgtttgacca
    10681 ctaaaatgtg ttacaaaact tgccgcaaat agcgctttac cttgtccatt cgtatggtta
    10741 actaaatcag cgatatcact gtgtttaata cttgcacgtt cggttgcagt aatcggtaat
    10801 tcaaatacca tttcatcgac ttcagaactt atttgtaata tatcgtttaa tatcacttta
    10861 ctattcgatt caaatgcagc agctttatca tcgcctagtg caacaattgc tgcacccgtt
    10921 aatgtagcaa agtccataat cacactaggc tgatattgat ttgcataaaa cacagcatct
    10981 gcaaggacta atctaccttc agcgtctgta ttcattactt ctacagtttc accacttaat
    11041 gctgtaaata catcatctgg cttcattgat gcttcattta tcatattttc agcacacgca
    11101 agcactccga caatatttac aggcagttgt aaacgactag ccgcttcaat gataccaacg
    11161 acattcgcag cgccacacat gtcaaacttc attgtagcca tgccattctt cgttttaata
    11221 ctataaccac cagaatcata cgttatacct ttaccaacta aggcaattgg tgcttcatct
    11281 ttgtctttgc cattatatgt gatggttact aatctcggtt tatgcttact acctttacct
    11341 actgcttgta aaagtccgaa tccttcagaa actaaagtgt cataatcttt aacatctact
    11401 ttgacctttg tatttttaaa atgattaaca atatcttctg caaatgtttg tggtgttaat
    11461 acattcggtg gcatattact aaagtctctt gccaaattaa tggattggcc aatactgata
    11521 ccctcatgaa taaaatctaa ttcaattaat gattcactaa taaggtttaa attcgtctta
    11581 aacggtgcct tcttacttga tttataatga tcgaattcat atgttgcacg ctcactttga
    11641 ataccgcatg ccattaatac atctgataac tgatcatatt ttgaaataaa tgaatccata
    11701 agtaaatacg tatcttcaat gtgttctgac tttatgtatt ggaaaagatg tccccatatt
    11761 ttcaacatat cttgataact acgtgtcttt aagtttccta aaccaactgt aattaaacga
    11821 tatgtttgat cttgtacatc aaatgcagtt gtataaatct tcccaacttt actaccaata
    11881 atatgttgat gttttagtct ttcaagtgat tctgtaatat cgatatgatt aaaactaatg
    11941 cgctctaact gatttaaatg ttctggtata ccaataatca atgtatttat ttcgttgctt
    12001 agtgtgttat ttaatttaaa attcataatg tacctcctta gatttgatat gttactcact
    12061 tcaaattgta caacaataaa gcccttcagt gacactgaag gactttataa atgaataatt
    12121 taattttatg tgtttagctg aaactttatt taaagcctag aatttacctt ttttgaatgc
    12181 taaaccgata ccaccgattt tgaataccgc acgtgtatca atcacttttt tcatgaatgc
    12241 tgctttttta ccagcgatag gtttaccaaa taccatacct acaccgtcat gtgaacctaa
    12301 agaacaaaca gttccacgat caacgtattc gaattcttca gttgattcac cgtttaagat
    12361 gcgtttaatg tttttagcaa cactttcacc ttgttgcatt gcaatttgtg ctgtagttgg
    12421 taatggacgt tcttctccag ctgggataaa cgctgaacag tcaccaataa caaaaatgtt
    12481 gtcgtaacca ttgattgtta aatcttgctt tgtaacgata cgtccacgtt taacgccttc
    12541 aaatgattct tccattaatt tactaccacg tacaccagct gcccatactg aagtacctgc
    12601 atttaattgt tgtttttcac catctacttc aactacaaaa cctttttcgt tacaagcaac
    12661 gattggtgta gcaattttaa attcaacacc gcggtcttct aagtagctaa ctgcgtggtt
    12721 aactaattct tctgagaaca ttggtaacat tttaggtgct gcttcaacac aagtgatttt
    12781 aactttattt tgatccacac catatttgct acataattca ggaattctgt ctgttaattc
    12841 acctaagaat tcaacaccag tgaatccagc accaccaact aagatagata aatcgttatc
    12901 atctttttct tttgatgctg catagttagc aaatttgtct tcgatatgac gtgataattc
    12961 acgtgctgtg ataacatttt caatttggaa agcatgatct ttcatacctt cgatgccgaa
    13021 tgtttcacta acgaaaccta atgctactac taaaatatca aagtcataaa taccttgatt
    13081 tgtttctacc tttttagcat cacggtcaat ttttgttact tctgcttgaa caaagttcac
    13141 tttgtctttc ttcaagacac tttccacagg atataataca tcttcatagt ttagtgtacc
    13201 tgctgatgct tcatgtaacc atgttgcttc atagtgatat tcatttttat taataagcgt
    13261 aatttctgct tcttctgttg atatcgcttt ttgcaattta gttacagttt gtaaacctgc
    13321 ataaccagca ccaagtacaa gtactttttt acgatcttga gccatttaat ttcacctaag
    13381 ctttcatatt tttttaacca aatgctgata attactagta acaactggtc attactactt
    13441 ttaccacaat gtatactagt ttatcatcat tttgttattt aagtctttta aattatgtaa
    13501 aaacctgtga tgttcacaaa aaagacattt ttaatccaat actaattcta tagcttttat
    13561 gttatatttt caagcaagat tcagtcagat tgtgaaaaaa ttaattaatt ttttcgtagc
    13621 ctgatttcga ttaaaaatgt atccgctatc acgatcattt aaaatactat ttatagtcag
    13681 attcttaatt ttcaataagc tttttaataa aacaaaatat ctttacgcaa aaataggaca
    13741 taatcaaata tgtccttaat cgttattgat atcaatttgt atcccaaaaa ttgggttaac
    13801 cattaaaaag ttattttatt agcaattttc aggattacct gcaacttttg cagttctaaa
    13861 tgaactacca cagccacatg aagcaattgc attaggattg tcgatttgga aaccgccacc
    13921 cattaatgat tgcttaaaat caatagtcgt accatttaat acgggtgcat cttttttgtc
    13981 tactaatact tttaatccaa agtattctaa gacttcatca ttttcaccag gcgcttcttc
    14041 tgcacccata ccgtatgtta aaccagtgca cccgccacca ttcactttaa tttttaaata
    14101 gccatctggc atttcatttg ctttaagcat atcttttact tcgtaagcag ctgcttctgt
    14161 taatataact gttggcatga taactcctcc ttaaaaaatc caagtttctt ttatatgtgc
    14221 atatatattt tgtaataatt cttccggcga atcaccttca acaatatcac catttactaa
    14281 agcatacaac ccggctgaac atataccaca atgtgtcagg caaccatact ctaacacatc
    14341 gacatctggg tcattttcca gttgattaaa aacataatct ccaccttttg ccatgttaga
    14401 gagacaaaat tctacgatcg gattcatact tcaccttctt atttcatttg ttacaatatt
    14461 atagcatttt aaaactggta ttttaacatg atgtgctcaa ttagcaacaa ctgatgtttc
    14521 ttatcccagt tatgtaatag tgccttagtt agtacaaata tttatttgta gtcattttca
    14581 aaaaaatata taataagcat ataaatacat atttactttt ttaggggtta ttgatgaatc
    14641 aaagacactt gcattttata actgttcatt aacaaatata tctatgcact ttgatctttt
    14701 caaaattaaa gtaaatatta acatataaaa ggggtacttc attatgaaaa acttagtttt
    14761 gttaggcggc ggatatggta atatgcgtat catgtcacgc attttaacta cttctttacc
    14821 acaagattat acagtcacat tagttgatcg tatgccattt catggattga aaccagaatt
    14881 ttatgcttta gctgcgggca cgaaatcaga taaagatgtt cgtatgaaat tccctaatca
    14941 tccacaagtg aatacagttt atggtgaaat taacgacata gatttagatg ctcaaattgt
    15001 ctcagtcggt aattctaaaa ttgattatga tgagctaatc attggtttag gatgtgaaga
    15061 taaatatcat aacgttccag gagccgaaga atatacacat agtattcaaa cactctcaaa
    15121 ggctcgggat actttccata gtattagtga actaccagaa ggtgctaaag tcggtatcgt
    15181 tggtgctgga ttaagcggca tagaacttgc cagcgaatta agagaaagta gatcagactt
    15241 ggaaatatat ctttatgacc gtgggccgcg aattttaaga aattttccag aaaaattaag
    15301 taagtatgtt gcgaaatggt tcgccaaaaa taatgttacc gttgttccaa attcaaatat
    15361 taataaagtt gaacctggta aaatatataa ctgtgatgaa cctaaagata ttgatttagt
    15421 tgtatggaca gcaggaattc aacctgttga agttgttcgt aacttgccga ttgatataaa
    15481 tagtaatgga cgcgtgatag ttaaccagta tcatcaagta ccaacatatc gtaacgtcta
    15541 tgtagttggt gattgtgctg atttaccaca tgcgccaagt gctcagttag ccgaagttca
    15601 aggtgatcaa attgccgatg tgcttaaaaa gcaatggcta aatgaaccat tacctgacaa
    15661 aatgccggaa ctaaaggtac aaggtatcgt tgggtcatta ggagataagc aaggttttgc
    15721 atatatcatg gaccgcactg taacaggacg actcgcttct attttaaaat ccggcgtact
    15781 ttggttatat aaatatcaca atggttaaaa ttaaaaagca atgtcagttc acactattac
    15841 tgacattgct tttagttatg tgtatattat tcattcacaa gcttttggtc tataaatcga
    15901 gtgatttgct ttgtttgtat ataaccatct gctacatatt catcgttcat tgtaattaat
    15961 ggataaaata gttcatcttg ttctattctt tcaataaatt gtaaatcatg atctgttaag
    16021 ttgtcattat cttttgtaat atctatatac gtatatttaa atgatatatt tgggtatttt
    16081 ctttttaata gcggctgtag ccagtcataa atatctttcg atgttggcgc attaacgcaa
    16141 cttgcacata taacatctgc cccataaact accacactca cgtgctccat atatttatcc
    16201 cccattggtt tgatagattt ttattacact atctattata atatactaat aaagattatc
    16261 aaattcatct ctcgaaagga gacttgcctg atgcctactg aagatacaac gatgtttgat
    16321 caagtagcag aagttattga acgtcttcgt ccatttttat tacgtgatgg tggcgactgc
    16381 tcattgattg acgtggaaga cggtattgtt aaattacaat tacatggtgc atgtggtaca
    16441 tgcccaagtt ctacaatcac tcttaaagct ggtattgagc gtgcattaca cgaagaagtg
    16501 cctggtgtaa tagaagtaga acaagtattc taatataaag atattaagtt aacagaacat
    16561 attatgaata acaacaaaga caggaatact gaattattta taatcagtta ttacctgtct
    16621 tttttcgctg tcaaattgga ctgatgggaa agttatttta tactatttat ctgattaatt
    16681 attttattat tgccacatat ccaagcacat attactattc tattctatga aatttaaaat
    16741 ataaaaatag cacatacgct caatcaaaat cgttgagtta tgtgctattt gtattttaat
    16801 ttttaggttt atctacttca ggttgtggtt cacctttcat atgtttcgca atttgctcat
    16861 ccatataaac ccaacctttc caaccgatgt gtacggcatc actgataaca tatttttcat
    16921 attctttatc agtcatatcg taaattttac caccattatc tacaacagta gaatggattt
    16981 ttttataaac tgcttgacga cgttctttat cgataccaat gtggtcatac catacaccgt
    17041 ttgatggaat acttacatat tgaacatctg caccagcagc acgcattgtt tttacaagta
    17101 attctaaatc ttggaattct ggagaattaa cattgaattc gtagtcacgt ctaactttac
    17161 gcttactttc ttgaattaat ttccagtatt gatctctaat accaaattta ttcgatgtag
    17221 tatcagcttt accaatttca actgcttttt gtttcatctc atcccaagaa gcgtctggtt
    17281 ttgtagcagg tttaacatgt tctaatggag atttatccat tgcaaacaat gatttaatcg
    17341 cttctatttt aatcaattga ttctctttaa aaccagaaat ataactatct ttagtttctt
    17401 tagggttttt agcataagat ttcaagtatt ctttattgtg tacatgtgga aactgtaata
    17461 aacgttgtgc ataacgacgt tttaattcag tagacatgtt tttctgctgg aacatttgat
    17521 taatttgagt ttgagacata cgagcatcaa agttttgatt cgttaaacca tggtttgtaa
    17581 accattgtgg tgaaataata aatgttaatt tcttaccttt taatttatca tactgtgatg
    17641 caagttcaac tgcgttaatt aagtctgtag aaccaccagc acctaataag aatgcttttt
    17701 tgttggcgtt atgcttattt aatgcaattg caggattaaa tgggtcatct ttacctaatt
    17761 cactagagcc gtatatagga taatacttgt ttgattcgta taacttatct tgaatgagtg
    17821 tgccttttag tacttgatct gtcaatgaag ttctattatc ttctacagtc ttttcattta
    17881 ctaatcctgt aaaccaacta gcaggtaata atagaaagac aatgaatacc gctccactaa
    17941 ttaaaatggg taaaaaaggt tttaatttca tcgtaactct tctaatgctt caacgatttt
    18001 atttggtgtt gcccactcat ctctatcaaa gtccataata gatacttcga tatcaagttt
    18061 attttgaatc tctaataata atccaactgt ttggaaagaa tcaataatac cttcttcaaa
    18121 aatttctacg tctggatttt cttttacaat atcattttct gctacttctg ctaataaatt
    18181 taatacttgt tctctaaatt ccataattaa attctccttt attatataag tttacctgag
    18241 aagattaaaa agccaaatgt tacaaagtgg aatgtaatca caatgctaag tgctgttgtg
    18301 aaaccatttt gccaacgtgg cggatgtttc ttacgccaac gttcataata gccataacct
    18361 ataaacaatg ctgcatggta taaaccataa acaatgtaat acacttcgat accatgccaa
    18421 attcccatta tgaagaagtt gattaagaat gccacgttag acattgcaaa ttgactcttc
    18481 aataattttt tacgagacat gtagaataaa gatctcatgt aaatacaatc tctgaaccag
    18541 aatgataatg tcatatgcca tctattccag aaatctttaa tatttttcgc tttgaaaggt
    18601 ttatcgaagt ttggtggtgt tttaataccg aataaataac taaatgctat cgcaaataaa
    18661 ctataacctg caaagtcaaa gaataaatat aagctgtatg cgtacatata taaccacaaa
    18721 ttgacaaagc catgtaagtc taattgtaac ggcatgattg catatgtgtt aataaagtaa
    18781 gcaacaatat atttatacaa gaaaccaagc ataatcatgt gaattgcttt taatactaat
    18841 tcacgatatt cattgcctgt tggtactttt ttatcgtctt taacgaaacg tttgtaacga
    18901 tcgattggtc cagatgaaat cgttgggaag aatgaaataa attgaattaa tttccatact
    18961 ttaatttctt tgatagaacc atcacgaatt tccataatta actgcacact tttgaatgta
    19021 acataagaaa ttcctaagaa accaacaaat tcaattaatt tactttcatg gaagtgaatc
    19081 tgatgtccac ctaaccatgt actttgtaac actttcacaa gtgctaatgg caatattgat
    19141 aaaaccatta cagttacaaa ttttgaaaat gaattatttt ttggttttga atgataataa
    19201 aacattatca ataaaacttg ccatacgacg taaataataa aactaattaa ttgaacactt
    19261 aaatactttt ggtcaaacag attatgttta tcagaagaaa agattaacac aatcataaat
    19321 gctgtaacta cgccattata aatgtaactt cgcttaccta aaaatccaag tataatgact
    19381 ggtaataatg caattaaagc aattaagaag aatgtaaaat caccatatgg aatcatccgt
    19441 taattacctc tgcaattttc tttctgtcaa ttttaccatt tgaagtcaat ggcaattgtt
    19501 ccatccattc aaactttcta ggaatcatat actctggtaa gcgtgatttc aagtcatttt
    19561 taatattttt agtcatttct gcattgtccg taacttcagt cgttggcaca attgcaccaa
    19621 ttaaatgaat aactttatca tttttatata caggtacaac aatcgcttct tttacgaact
    19681 cagactggcg taattgtgtt tcaatttctt ctaattccat tctgtagcca ttcaatttga
    19741 tttggaaatc aatacgacct tgaatgaacc attgaccatt ttcaaacttc gctttatcac
    19801 cagtgtgata tgtacgaata ccgtcatcga aattaaatac ttcagctgtt ttttggtcat
    19861 tttttaagta tcctaaactt acactttgac cttcgataac aagttcacct tcatctgtag
    19921 tagataatct tgcgcctggt ctttcaacgc caacaggtaa tgtcggatat tgatctaaga
    19981 tttcttgtgt aatttgaata cttgtaactg ctaccgtagc ttcagttgga ccatatgtgt
    20041 tgtaaatcgt cgcacttggg aaacggttta ctaacgcttt tgctgctctg tgaggtagaa
    20101 tttcaccaca gaagaagaat tcgttaagac taccatattg ttcttcatta agcgttggta
    20161 ataataaaca catttccata aatgatggtg ttgataccca aatgttaatc ggtgttgctg
    20221 ttagcatttc atttaataat ttaggtttat taatcatgtt tttatctaca agatttaatg
    20281 taccgcctga tgctaaacat ggataaatag ccattacaga taaatcaaat gaaaatggcg
    20341 cttggttaag ccattgttgt tcatttcctg atttattaag ttctaacatc cactcagtaa
    20401 attgaactaa acttgcatat tcaatttgaa cacctttagg ttccccagta gaaccagatg
    20461 taaagattgt gtatactgtg tcgttatctt taatctgact atcaaaaatt actgggtctt
    20521 gagatgtttt aatatcttct attgtaaata cttcgccttc taaactttca aatgattcat
    20581 cagtcgtatt aaatacaaac tctggttgaa ccttgttaat aatcatttta atacggtctt
    20641 caggaattga agtgtctaca ggtacatatc cacatcctgc tttaatggca ccaatcatcc
    20701 caacaatcat atatggtgac atgtgaccga ataaaatcat cggtttctta ctaccttgta
    20761 atcgatgtgc taatttacta gactcatcca ttaactgttg ataagttaat tcatcagttg
    20821 tgtgtctaac agcaatgctt tgtggatttg catccgcaaa cgcttgcagc ttgttaataa
    20881 tatctgtcat attaagtctc cctcattaga actcattata aatgaagtta ttgtgtgtgt
    20941 cgccactgcc ataaattaaa tataaagtaa taaatattgc caaatacaat agtgttaata
    21001 aatatggttt gaatgcttca acatatttat taggtggctg tttactttta gatttcatat
    21061 tgcacctctt aaagttctta gtaaaaacgc ctttataaag accgttcaat ataaaatacg
    21121 ttttaaaatt tgttttttac aattcattat atcgatattc ataatgaaat tcaattttaa
    21181 ttttatagat tcaacatagt aattggttgt catcactcaa ttattgtaaa tgatacactt
    21241 tgttgagaac atcattcatt ttaaaggtta tttaaacaat aaacaattac agtctatata
    21301 acaattttgt tatatacgtc aaaatcaaat aaactcatca cattaatatg acgagtttat
    21361 aatgttattg aattatcatc agcgcaaata tatacattcg caagtcaagc ataacatatt
    21421 taacaattgc tttgcttgtt ttaccaatga ttaaaaacca tacttatttt caatttactg
    21481 gagtatgtgg tacctgattt gtcataaccg cttctatatt attaatacat aattgaatca
    21541 tattgtcccg tgttgttact gatgcgctac caatgtgtgg tgtaatcaga acattatcac
    21601 gtcccattaa tggatgtgta tgatcaatcg gttcatttgc taatacatct aaaccacatg
    21661 ctaaaatttc tttattgtct agtgcatcga ttaatgctgt ttcatctaca atttgtcctc
    21721 taccgatatt aataaaaatt gcatcatttt tcatttgttc aaatgcttca gcattaaatt
    21781 tatgatgtgt ttcttttgta agtggcgctg tacagatgat aaaatcactt tctgctaaca
    21841 acgtttcaaa agaaacatat gttgcattaa agtccgcctc tgcatcttta tgtcttgatc
    21901 gattatgata aagaatatta gtattaaacc cttgcaacct tcttgcaaaa gctttaccaa
    21961 tatctcccat accatatatt ccaatagttg aattgaagac atctttacct gacaataaat
    22021 aaggacccca gctttgccat gcatctgctt ctacatattt ttcagcttct acaatacggc
    22081 gtgctatagc aagcattaat gtaaatccta attctgcagt tgtttcagta agtacatttg
    22141 gtgtattcgt gacaaccacg ttattcgctg ttgcactttc aacatcgatg ttgtcatatc
    22201 ctacagccat attcgcaatt acttttaaat ttggtgattg cgctaaaatt tctgcatcga
    22261 tctgttcact taatgtaata aaacaagcat ctgcgtcacg aagtgctgtc acaaattgat
    22321 ctttaggcat aggcactaat gatttttgcc acataacaac gtcaccaagt ttacttaatt
    22381 gttgataaaa tttatctgga attttcctcg aaacaactat ttttaccaaa tcaattcacc
    22441 tactttataa aatgactata tctatttttc taattcagct attgcttcat ttaaatcttt
    22501 aaaagaatac gttggtggca catttttatt ttgcacatct tctaacgtag atacacctgt
    22561 ttgtacatga atcgtatcca tacctacgtt aataccagac ataatatcgg tatcgtacaa
    22621 atcgcctacc atagcaactt cggatttatc taatcctaaa atttctaatg ctttaaccat
    22681 aataatcggt tctggtttac caataaattg tggcgataca cctgtcgata cacttacaac
    22741 acttgtaata gcaccattac caggtaataa accacgctct ttaggaattg atacatcagg
    22801 atttgtagaa ataaatgttg caccatttct tacacctaac gttgcaatcg caagcttttc
    22861 atatgtaact tgttcgtcaa gtccaataac tacataatca acatgctcgt catttttaat
    22921 gacaagtccc gcttcggtta acgcagtatt taaaccactc ccacctaaca tatatactga
    22981 tgctcctggt gattgttctg aaatataatc agcagtggct aacgctgacg ttacaacctc
    23041 ttctggttta gcatcaatgt gcatttcacg taatttttca gttacttgct caggtgtttt
    23101 tgttgaatta ttagttacgt ataaatgagg aatgcctttt acatttaaat aatcgatgaa
    23161 ttgttttgct ccatcaatct catctgttcc catatacatt gtgccatcta aatcgattaa
    23221 atacgcttta tactgtttca ttattgattt tctcctttac caaaagctgt tactggtaca
    23281 ttttcttgat gtaaaaatgt cgttacttct gtaataaatt gtttgtaata aggtaatgcg
    23341 tcatcaaaca ttggcatgat aatcttaata tctaaggctg tgtaatcata tgtaaattgt
    23401 ttacgaatac cgacagtttt attaatcgcc tgctgtgttt ctttagtaat aacattttct
    23461 agttctaata tatcaatcac atctttataa ttaccaggat cccttaaaat aaatgcatcg
    23521 ataatcatat tccctatatc taccgatgat tctatcaaca tttgagcaat gcgttcaaat
    23581 gcataatgat tgtgcttgct ctcatgataa tcatcagtta atgcttgtaa ataggctaat
    23641 ttctgagtta gtttatcttt gtctacaaaa tacattcctt gtcacctctc acttatatat
    23701 tatcataatt ttgcagtata cttcatttgt cgttatacta actttactac ttttaaaagg
    23761 agtcgctaca aaatgattga tatgtattta tatgatgaca acgaagagag tcaagttcag
    23821 tttgttggtt ttgttggcga gcatagtcga tatgatttga tgttagtcca tacaaataga
    23881 cattatggta agacactcgt acttaatatg caaacaaata aattcggtat tatcggtact
    23941 gatgatttaa aagaagaagg ctatatcgct catattttag gtgtaaatgc tgaagaaggc
    24001 gatgaaatta ctgagtattt aaatgaagtc attcattaaa gataaaaacc cctaactaga
    24061 ttacaggtcc atgcttgaat tgcatgattc ccattttcaa tctagctaag ggttttatta
    24121 atgttcagta tcttgaatgg ttggcttaat cttcacatct tgaagtttat tgtcgtcatc
    24181 tatcacatca gacggatgaa cttcttcgat taacttttta acctcttgtt tagacaaatg
    24241 tcttaataca aagtaaggac aaccaaagtt acaatattca aatatgtaat cttgaatatt
    24301 tgaaaaacgt ttactcatct ctgctttttt attagaatct ttgtaaaaac cttttaatcg
    24361 taattgatcg taaccatagt caccaactat gtaatcatat ttatctaaaa tatctgaata
    24421 cctagcaata aattgttctt cattaaaaca ttcgcgataa ttttctatta attcaaagta
    24481 atgttgatct acttttatca aattcttcac ctatctatga caatgaaagg ctcagtttat
    24541 acttttagtg tttcataaaa ataatgatta cttctatgac acgttcgttt tgtacaaact
    24601 aagcctttat gccttattaa tggttaaata ttaactattt aactgtgcct caccttggcg
    24661 ttgcttttct ttagcagctt catttacttg ctcatccgca tgataagaac tacgtactaa
    24721 aggtccagct tggcaatgtt taaacccttt atccattgcc acttttctta atttaccaaa
    24781 ctctaaaggc gtgtaatatt tttgaacctt taaatgttta cgtgaaggtt gtaaatattg
    24841 accaatcgtt aaaatatcta catcattcgc acgtaaatca tccatcgttt cataaatttc
    24901 ttctatagtt tcacctaatc caaccataat acttgattta gttgggatat ccggttgtaa
    24961 ttcttttgaa cgacgtaaaa actctaatgt tctgtcgtaa gtcgcacgcg cacgaactct
    25021 cggtgttaag cgacgaacag tttcaatatt atggtttaaa atgtcaggtc ttgacgccat
    25081 taatgtttct aacgcatcat agtccccgcc catatctgat ggtaaaattt caatcgttgt
    25141 aaatggattt ctttctctaa ctttacgtac tgtctcagca taaacatttg aaccagcatc
    25201 tcttaaatca tcacgcgcaa cagcagtgat aacaacgtgt ttcaaattca ttaattcaac
    25261 tgattcagct acacgttcag gctcatttaa atcaagttca ttaggtaaac ctgtcttaac
    25321 cgcacaaaaa cgacaagctc ttgtacatac ggcacctaaa atcataaatg tcgctgtacg
    25381 acgtgcaccc caacattcat gtatattagg acatttagct tcttcacata cagtattaag
    25441 atttttttcc ctcatcatct tcttaagtcc tgtatagttt tcgttggtat ttaattttat
    25501 tttcaaccaa tccggtttac gtaatatttc ctcgtttttt gtcgccataa caacacatac
    25561 cctcctgtta agtgtatctt taaacattat atcgaaattc atgacaaatg aaaatccaat
    25621 aatcttactt tttaatattc caataatttt tctttaaata tatctcttaa aaactctggc
    25681 attaaatact cttttggtaa ttctttcaat gttgggaaat aacgaccgaa cgtatacata
    25741 tctatcgtac ctagtacata ttctttgtcg tcttcgattt cttctccatt cagatagtaa
    25801 cgccctgtag aatgaatgta ccctaaatta taaagaatat atcctccaaa tatattccct
    25861 ctgaaaccca aaccttgtgc atgttcatac atatattctt gtttattact tttagctata
    25921 atctctttta atttcacacc aaaaagtcta acccttacca tattaatcgg atgcggtaac
    25981 atttgatgaa tgtcatattc tgtcacttca tcttttacaa tatctttaac gagtaagcca
    26041 gcattgatga tggcacattg tgcatgtgta tactcacaaa cactttgagc taataagtat
    26101 gcagcttcag ttatgtgatt catactacgc ttaagcacta ctggatgttg aattactgaa
    26161 ttggacatta acgtttttcc ttcttcttca aatgaagttt caacttcagg taatgtttct
    26221 aaaggaataa tctttgcagt tttatgtact actttatgtg cctcaaaagt taaattaacc
    26281 tctccaagat aattaccata ctttccagct gccgccatca gtacaccatt attgatttca
    26341 ccatgttcaa aataatgatg cgtatgacta ccaaaaatga catcaatttc aggcaattct
    26401 tggcataatg tttcatcgaa gaaaatgcca caatgactta gcacgattaa tacatcaaat
    26461 ttacctcgtt gaagttcaat ttcttctttt atagattcaa gtggatcggt aacaatccaa
    26521 tttagtgcac gataaaatgg ggtaaaaggt gctgtcgctg caacgaatag tattttcaca
    26581 ccgtctatgt ccttaatata agaagaaacg atattatttg gtaaatgacc tgattcatct
    26641 ataacattgc tacatgtcac tataaatttt gcttcgtcgt aaaggtgatt taaagcttcg
    26701 tgtgaaatgg tcatcccttc attattaccg attgttgcaa catcacattt tgcttcattt
    26761 agtaatgcca cattcttttt acctaaagtt gcttcagtta taggtgcgga taaatctaca
    26821 tgatcaccta gatcaacata taaagaagga tgattaagtc gtggccgatg ttctgccata
    26881 tatgctttaa tgcgttcgta ttcatgtaaa tgactatgaa tatcgttcgt atgataaatt
    26941 gtaagcctca aattgacgtc ctccttttaa agttgtatta aatcaacgca cgaataatta
    27001 aatatatacc cattaacaac attactgttc tcaataatgt tactaccgta tctgacttaa
    27061 ttgattgatt cactttgaca ccgattttcg caccaaaata actagaaata attaaaatga
    27121 ttgcataacc ccaagctacg tgaccttgag caatgtgccc tatagaactc atgacacttg
    27181 aaaagaaaat catcatcata cttgttccaa cagctacatg aggtggaaat ctaaatacaa
    27241 taagcattag tggcgtcatt agtgcgccac ctccaatacc aaataaacct gtcaatatac
    27301 caattaaaaa cgttgtaata aaagcaaaca atggtggaac actataatga tatgttttac
    27361 ctttagcgtc tacataagtc ttttcatact tgggtttatc gaaaatttta aacggtttaa
    27421 tcttatttct taccattaat aaaatggcta cgaaaattaa aaagatacca aaatataaat
    27481 taaatgactc aaatgttaaa tatctactaa tgaaggaccc aagcaatgaa cctggtaata
    27541 gtccaaataa aaagatggaa ccatttttaa tatcaacttg ttttgttttt aaatatccaa
    27601 gtgatgaaga aagtcctgtc acaattagaa tgactgaaga cgtccctatc gctacttgtg
    27661 ttgtaatatt atgtagtaat ccatgttcaa caccgaggta aaccattgtt ggaacgataa
    27721 taataccgcc tccaatgcct acgatagacc ctataatcgc tgacaaacct ccgattaaaa
    27781 ctaataatgt aattgttaat aacatagtcg tcgcctctta aaataatttt aattgttgtg
    27841 gtgctaaccc ttcatattct atattcaata atcgctgata tgttttggca ttatttgctg
    27901 catgaccacc agaattattg ttaaatatga cgtaaacttt cttagctttt tgtgctaata
    27961 tttgtgcctt ttgtgccaag tctattaatt cttgctcatt ataatcatat aaatagcgta
    28021 catcgcgcca ttcttgatct gacatatctt tcttagtcca accgtaatga ttacgtccat
    28081 gataacgtac aaacgcaatt tcatttgtga ttcgattgac taaaggtaca ctgccatctt
    28141 tcacttgtgg ttcatcaact actgcatgaa tgatttgatg ttctgttaaa aatgccaatg
    28201 tttgttcttt aaatgcatca ctaaaccatg attgatgcct aaattctaca cacattggaa
    28261 atgcttgtaa ttgctgtctt acatacaaaa tatatttgat attttgtgca ttgcagtcaa
    28321 accatggcgg aaattgaacc aataccattg ctaatttttt ctgtgtatgt aagggctcta
    28381 acatattctt aaattgatca aatagttctt gccttgtatc tgcaaatgtt ttgtagtctg
    28441 catgcaatgt gagtgcttga tgaattttga ccacaaattc aaatgtatca ggcgtttctt
    28501 ttatccattt caatatattt ctttccggtt gtatcgcata gtatgtcgca tctaattcga
    28561 caaccggaaa atgtccagca tatgttttaa gtttatcggt ttggcgttct aaatcttcat
    28621 ataatgaata gtgatcaccc caacctgtta atccgatgtt tatcatatat atcaccaatg
    28681 tcatcatacc atataacttt tatcataatc atttcagcga actttagttt gatgtttttg
    28741 cttgattaaa atctttcggc gatcttcagc ttgatgtttt cgtttgatta aatttgtaaa
    28801 atagaaacgc aatccacaaa aatggcaagc actaaaataa tgtttggggg tgcttgtgct
    28861 tttgtggatt gcggtcgatt atttatattg catgatttga ttaatttgat tgattatatt
    28921 ggacatgatg gtgttggcgg gatgcgttgt tgctagtcgc gggctttgtc cactccacat
    28981 atgtattaac tctttgtcgc cgatgtttgc tgcggctttt cttatgctac ttgttagctc
    29041 attttgtatt ggataatctg ggatatcgcc ttcgtattgg gacatttctt cgataaacct
    29101 attgttgata ccgcgtgcaa gctttccact aaacgctttt gtaatgactg tatctgtttc
    29161 tttactattt ataattgcat ctcgcagtag ttctgatgca ttactgtctt gtgatgttaa
    29221 aaatgcggtg cccatttgta ccccttctgc acctaagaca atacttgcca aaactcctct
    29281 accatccata attccaccag cggcaatgac cggaattgaa acgacatcta caatttgtgg
    29341 cactaaagat attgttccaa ccataggtaa ttgattttta ggttttaaaa atgaaccacg
    29401 atgtccacct gcttcactac cttgagcaac gatagcatcc atacccgctt tttcattcgc
    29461 aatagcttca tcaacacttg ttgctgtacc tataagtttg acattcgctg ctttcaacct
    29521 gcttataatc tgttcgcttg gaattccaaa agtaaaacaa catacaggca cttgcttttt
    29581 aattatcgta tcaatatgac acttaaattg ttgttcttcg gtaattttta caaccggctc
    29641 ttctaaatgt aatgcgcgtc gataaggttt taaccatgca ttcatatttt caatttgact
    29701 actggtatat gattgttgac ttggtacaaa gacatttacg ccaaaagaat ttgacgttaa
    29761 ttggcgtaca taatctattt catcttccaa ttgctgcgta ttaaagtaac ctgcgcctat
    29821 tgtgcctaac ccaccactgt tacttactga tgcaactaat ttcggtgtcg tacttcctgc
    29881 catacctgct tgtataattg gatattcaat acttaacatt tgagtaagtc gattcttatt
    29941 ccacatagct gttcgctcct tatatagata cgttgcgatt tttccgttgt tgaaattgaa
    30001 tttgctgttg agaaagtttt tctttttcct ttttatccat ctcatcttca atttccatac
    30061 ctaataattc ttcaattaag tcttcatgtg acactatcgc ttcagtacca ccaaattcgt
    30121 ccaacacaat tgctaaatgt tttctagaaa tagtcatctt acgtaatacc cattcagctt
    30181 tattgtgttc attcacaaat aatggcttag ctgaatagtt tgtaatttga ttttcttttt
    30241 tattactcca agccaacaga tatttagaat gaaacacccc aataatgtta tcaatatctc
    30301 cctcgtacac tggatatcta gtgtatggct tattcataac cgtttcataa acttcttcgt
    30361 atgtcgcatt tgaagcaaat gccgtcacat taattctagg tgttgtatct acatctttta
    30421 cttttaaatt ttcaaaatta atgacacctt ccaacctact cgtctcaatt tcatttaaag
    30481 caccttcatg tccagcaatt gctaacattg ttttaaattc ttcttttgaa aattgatgtt
    30541 cttgaggttg gcccttagat aaacttcgat taatactgtc cgtcaactta tttaaaagta
    30601 atgtgatagg acggaacaca atgacacaaa tattaataat tggatataca agccttgtta
    30661 ttttatctgg aaatgttgca gcgacagact tgggaatcac ttcggagatc aaaatgataa
    30721 caactgttaa aacagctgat gcaataccaa cgctaatccc ccaacgtaaa gccataattg
    30781 taacaagtgt tggtaataaa atattcgcga cattattccc aattagaatc gttgtaataa
    30841 actcacttgg tttttcaagt aactttacaa tgccttttgc ttttttatca cctttgtcag
    30901 cttcagtttt aaattttgtt ttattggcag ccgttaatgc cgtctcgctt cctgaaaaga
    30961 aaaacgaaat aaatatcaat ataattatgg caatgatcac gcgtgtagtc tccttattgt
    31021 catatcttat ttttattgta gttacttaat tcccgacgtt ctcgtttaat aaaccagtta
    31081 aaattcatta tatataatag tcttaataat gataattaat atgcattttt agtcatagtt
    31141 actttaatgc attcattagt aaaatgatgc tatagttcga gctgtttgtt attaataaat
    31201 ttgcataatg aaaaatttca acaaattcag tttatgacat agttataatc atttgaaacc
    31261 attataatga taacaaccag tttctcataa atttattcta attatctttt aaataacttt
    31321 agacaagtac ttgtttatat ccaaatatag aaagaaggat taacatgtat tttgttttag
    31381 caatatttac aataatcagt gccagtgtaa gtttaggtta ttcaattcaa gcatgtgcat
    31441 ctagccataa tataaatgca tattatgcac ttagtcgaag cttaccttta tttttattag
    31501 ctattttttc tttagtcatt catagtgcta tatttttgat aactatatcc attgcaatga
    31561 tcttagttca atttttagat gcgattgttg gttataaaag tgaagatgtc tttaaaactt
    31621 atggtccatt agcaacatct gtagtgaact taatattatt aatagttttc ttattttaac
    31681 tcacttatac aacgaatctt aatcgaacta atatcgaatt atctaaagaa tatttgatgt
    31741 agctttcaat taatttaata aagaccagca ctctaatgcc acaatcatgt tgtatttgtg
    31801 ttgtcgcttt atccaccatc aatgattatt ttttacacca atcaaaaaat cgaactgata
    31861 taaataagta caaagcttat ctatcaatcc gatttagtta taaaacaaaa aaagccacag
    31921 taatgtggct ttttgttata ttcagtatca aaatggtatc aatagccatt ttcggaagtc
    31981 aagaatggct taacaacgcg gtttaaagct atccaatact accttccatt tcgaacttga
    32041 ttaaacggtt catttcgacc gcgtattcca ttggaagttc ttttgtaaat ggttcgatga
    32101 atcccataac aatcatttct gtcgcttctt cttcagaaat accacgactc attagataga
    32161 ataattgttc ttcagaaact tttgaaacct tggcttcatg ttctaatgat atttgatcgt
    32221 tgaatacttc gttatatgga attgtatctg atgttgattc gttatctaag attaatgtat
    32281 cacattcaat atttgaacga gcaccttttg ctttacgtcc aaaatgaaca ataccgcgat
    32341 aaataacttt accaccattt ttagaaatag atttagaaac aattgtagaa gatgtattag
    32401 gtgctttatg aatcatttta gcaccggcat cttgaacttg tcctttacca gcaaatgcaa
    32461 tagataatgt actacctttt gcaccttcac ctaaaagaac acagtttgga tatttcatcg
    32521 ttaacttaga acctaagtta ccatctaccc attccatatt tccgttttca taaacaaaag
    32581 tacgttttgt aactaaattg tatacattgt tcgcccagtt ttgaatcgta gtataacgaa
    32641 cgtgcgcatc tttatgcaca atgatttcca caacagcaga gtgtaaagaa ctagttgtat
    32701 aaactggtgc agtacaacct tctacgtaat gtacagaagc accttcatca gcaatgatta
    32761 atgtacgttc aaattgaccc atgttctcag agttaatacg gaaataagct tgtagtggcg
    32821 tatctagttt gatattttta ggtacataaa tgaacgaacc acctgaccat actgctgagt
    32881 ttaacgccgc aaatttgtta tctgctgcag gtactacaga agcaaagtat tttttgaata
    32941 attcttcatt ttcttgtaaa gcactatctg tatctttaaa gataatacct ttttcttcaa
    33001 gttctttttc catattatgg taaacaactt cagattcata ttgagcagaa acaccagcta
    33061 aatatttttg ttcagcttca ggaattccta atttatcgaa agttctttta atttcttctg
    33121 gcacttcatc ccatgaacgt tcagcttgtt ctgaaggctt tacatagtaa gtaatgtcat
    33181 cgaaattcaa ttctgataag tcgccacccc attgaggcat tggcatttta taaaacaatt
    33241 ttaatgattt aagacggaaa tctaacatcc attccggctc atttttcatg ttagaaattt
    33301 ctctaacgat attctcagtt aaaccacgtt ctgatctgaa aatggacaca tcatcgtcgt
    33361 ggaatccata tttataatcc ccaacatcag gtgctttttt agccatttca atcactcctt
    33421 aaaaatatat ttacaacggc gtgcgtaagt ttttaattca tgatgtaaac catattataa
    33481 caatgacatg acatcttata aaaattttta tacttttata tgtctaatat caaaattatc
    33541 tatgattaac agcattctat tcttcttcag tcgtaccttc tgctttacct tctttagcaa
    33601 cagtaccttt ttccaatgct ttccaagcta atgtggcaca tttaatacga gctgggaatt
    33661 gagatacacc ttgcaatgct tcaatatctc ccatttcttc tgtaatcaca tagtcttcac
    33721 caagcatcat tttcgtaaat tcttggctca tttgcattgc ttctccaagt gaatgacctt
    33781 taacagcttg tgtcatcatc gatgcacttg ccattgaaat cgaacaacct tcaccttcaa
    33841 acttagcatc ttttataatg ccgtcttcta tatcaaatgt tagtcgtata cggtcaccgc
    33901 atgtcgggtt attcatatct actgtcatag acccgttatc taatacacct ttatttctag
    33961 gatttttata atgatccata atgacagatc tatataattg atctagatta ttaaaattca
    34021 taagagaaaa actccttcgt ttgtttcaag gcatttatta actgatcaac gtcttctttc
    34081 gtgttgtata tataaaaact cgctctagct gttgaagaca catttaacca tttcattaac
    34141 ggttgcgcac aatgatgccc agctctaacc gctacacctt ctgtatctac ggctgtagca
    34201 acatcgtgtg gatgtacatc ttgtaaatta aacgttatta cacctgcacg acgatccttt
    34261 ggcgggccat aaatttcaat tccttcaatt gcagacattt gctcataagc atatatcgtt
    34321 aattcttgtt catatttatg aattgcatca aaacctatgc gttctaaata gcgaatagct
    34381 tctgcaagcc caattgcttg agcaattaat ggagtacccg cctcaaattt agtaggtaaa
    34441 tcagcccatg ttgcatcata cttacttaca aaatcaatca tgtcgccacc gaactcaatc
    34501 ggttccattt tttgtagtaa ctcacgttta ccaaataata cgccaatacc tgttggtcca
    34561 agcattttat gaccactaaa actataaaaa tcagcattca tttcttgcat atcaagtttc
    34621 atatgtggtg ctgcttgcgc cccatcaaca ctgataattg caccatgttg atgagctatt
    34681 tctgcaatgg ttttaacatc attaattgta ccgagcacat tagatatatg tgcaatagca
    34741 acgatctttg ttttatcatt aatcgtttgc ttaatatcct cgatgtttaa ttcaccgtca
    34801 gctgtcattg gtataaattt caatgtcgca tttttacgct ttgctaactg ttgccaagga
    34861 acaatattgg catgatgttc catttcagtg acaacaattt catcgccctc ttcaacattt
    34921 gcatcaccat agctatgtgc tacaaggtta atcgacgcag ttgttccgcg tgtaaaaatg
    34981 atttcttcaa aatacttcgc attaataaaa cgacgaacgg tttcacgggc attttcataa
    35041 ccatcagttg ccaatgatcc taatgtatga acaccacgat gaacgtttga attataacgc
    35101 ttgtagtaat cttctaaaac atttaacact tgcacaggcg tttgacttgt cgctgttgaa
    35161 tcaagatatg ctaaacgttt gccattgact ttttgatcta atatcggaaa atccttgatt
    35221 acttcattaa cgtcaaatga gtgttcggcc acttcattca cgacctttct taaataaaaa
    35281 tcctaatcat ttaaatactg acgttgtatt agtcttatac caatatcgac agtctatatc
    35341 tattacaaac ttttattttc aaaatattat ttagaaactt tgcgttcaat tacttctctc
    35401 aattgacgtt taacgtcttc gataggtaat tcacgtacta ctggatctaa gaaaccatgt
    35461 ataacaagac gttccgcttc tctttgagaa ataccacgac tcattaaata gtaaagttga
    35521 tctggatcaa cacgacctac tgatgcagca tgaccagctt gtacatcatc ttcatcaatt
    35581 aataaaatag gattcgcgtc accacgagca tgttcagata acattaatac acgtgattcc
    35641 tgattagcaa ttgatttagt tccaccatgc ttaatgtagc cgataccatt aaatacagac
    35701 gatgcatgtt ctttcataac accatgttta aggatataac catctgtttc tttaccatat
    35761 tgtacgattt tagatgttag attaattttt tgttcgcctg tacctacaac tactgattta
    35821 agtgaacttg ttgaacgatc accaaataaa tttgttgtat tatcaataat ttggctaccc
    35881 tcattcatta aacctagtgc ccaattaatt gaggcatccg cttcagtaat accacgtcga
    35941 atgatatgac ctgtaaagcc tttatccata tagtccactg agccatatgt gatatttgaa
    36001 tttgcaccag caatcacttc agaaataata tttaattgat ttccttcacc agatgcattt
    36061 gataagtaat tttcaacata tgtgacttcg gcgctttctt cagtaacgat gataacatgg
    36121 ttataaaagc ttgcattttc gtcgtcgtgc aacacaacgt attgtactgg atgttctaca
    36181 actacatttt taggaacata aacaaatacg ccaccattaa ctaatgccgt gtgtagcgct
    36241 gtgatacgat gttcatctac tgttactgca tctttcataa agtacttttg tactaaatca
    36301 ctatggttca taagagcgtc tgctaaacct tcaacgataa cgccatcttt cgatgcatta
    36361 tcatcaactt gtgtgtacgc aatcgtatta ttatgttgaa ttactaagtt tttagaatga
    36421 tctacgtcaa taatttctct tactgactca ggtaattgtg ataaagattg ataaacatca
    36481 ccttttacat cgtgttgttt aaaagaatca aaatcccatt ttcttaattt tgttttatca
    36541 ggttttggca tttctaaagt ttctgttaat ttcaaagctt ttttacgtaa ttctgtcatc
    36601 caagaaggtt cattgtgggc tttagaataa tcaacaagtt gttcttcaga aatgttcaaa
    36661 atatcagttg tcataacttt ttcctcctac ttgatgaaat ttactatttc gtataaatgt
    36721 tcattttcat catatataga tgaacatctc atggatactg tattaataag attattcagc
    36781 tgaaccgaac tcttctttaa cccattcata tccttcttct tcaagacgtt ttgctaattc
    36841 tggaccacca gatttaacga ctttaccagc atacattaca tgtactttat caggagtaat
    36901 gtaatttaat aatcgttgat agtgtgtaat cattaatgca ccaaagtttt ccccacgcat
    36961 ttggttaata cctttagata caacttttaa tgcatcgatg tctaaccctg aatcgatttc
    37021 atctaagatt gcaaacttag gttctaacat cattaattgt aagatttcgt tacgtttctt
    37081 ctctccacct gagaaacctt catttaaata acgttgtgcc atgtctttat ctatgtctag
    37141 aaaatccatg tttttatcta atttcttaat aaattgcatt aagttgattt cttgtccttc
    37201 ttcacgtttc gcattgattg ctgaacgcat gaaatcagca tttgtaacac ctgtaatttc
    37261 tgatggatat tgcattgcca agaataatcc tgcttttgct ctttcatcaa cttctaattc
    37321 taaaatattt acaccgtcta aaagtacttc tcctttagtt acttcatagc ttgggtgtcc
    37381 cataattgca gatgataaag ttgatttacc tgtcccgttt ggtcccataa tcgcatgtat
    37441 ttcatcagtg ttaattgtca agttaacacc ttttaagatt tctttatcct caatagacac
    37501 atgtaggtct ttgatttcta atgttgatgc cattttatat tccctccgta aaatataaag
    37561 ttttcttaac tagtttataa taattttaat ttgtagtcaa aaagactttg taataatgcg
    37621 ttcagttaat tataacttac ttatacctta atataaacaa cttaaaccct ttttatta
//
LOCUS       DANJFZ010000015        36845 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.015, whole genome shotgun sequence.
ACCESSION   DANJFZ010000015 DANJFZ010000000
VERSION     DANJFZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.015"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..306
                     /locus_tag="PWN45_000623"
     CDS             <1..306
                     /locus_tag="PWN45_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0622412.1"
                     /translation="TFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGRFFIE
                     GLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTKKVK"
     gene            314..799
                     /locus_tag="PWN45_000624"
     CDS             314..799
                     /locus_tag="PWN45_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0622413.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            807..2246
                     /locus_tag="PWN45_000625"
     CDS             807..2246
                     /locus_tag="PWN45_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0622414.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            2313..3248
                     /gene="trxB"
                     /locus_tag="PWN45_000626"
     CDS             2313..3248
                     /gene="trxB"
                     /locus_tag="PWN45_000626"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL0622415.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            4128..5039
                     /gene="rapZ"
                     /locus_tag="PWN45_000627"
     CDS             4128..5039
                     /gene="rapZ"
                     /locus_tag="PWN45_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL0622416.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            5036..6031
                     /locus_tag="PWN45_000628"
     CDS             5036..6031
                     /locus_tag="PWN45_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL0622417.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            6141..7085
                     /gene="whiA"
                     /locus_tag="PWN45_000629"
     CDS             6141..7085
                     /gene="whiA"
                     /locus_tag="PWN45_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL0622418.1"
                     /translation="MSFASEMKNELTRIDVEEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            7659..8246
                     /gene="clpP"
                     /locus_tag="PWN45_000630"
     CDS             7659..8246
                     /gene="clpP"
                     /locus_tag="PWN45_000630"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL0622419.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(8489..9391)
                     /locus_tag="PWN45_000631"
     CDS             complement(8489..9391)
                     /locus_tag="PWN45_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL0622420.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            10093..10722
                     /locus_tag="PWN45_000632"
     CDS             10093..10722
                     /locus_tag="PWN45_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDL0622421.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            11364..12377
                     /locus_tag="PWN45_000633"
     CDS             11364..12377
                     /locus_tag="PWN45_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDL0622422.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            12430..13440
                     /gene="gap"
                     /locus_tag="PWN45_000634"
     CDS             12430..13440
                     /gene="gap"
                     /locus_tag="PWN45_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0622423.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            13579..14769
                     /locus_tag="PWN45_000635"
     CDS             13579..14769
                     /locus_tag="PWN45_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL0622424.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            14891..15652
                     /locus_tag="PWN45_000636"
     CDS             14891..15652
                     /locus_tag="PWN45_000636"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL0622425.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            15655..17172
                     /locus_tag="PWN45_000637"
     CDS             15655..17172
                     /locus_tag="PWN45_000637"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDL0622426.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            17302..18606
                     /gene="eno"
                     /locus_tag="PWN45_000638"
     CDS             17302..18606
                     /gene="eno"
                     /locus_tag="PWN45_000638"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL0622427.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            18946..19404
                     /locus_tag="PWN45_000639"
     CDS             18946..19404
                     /locus_tag="PWN45_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622428.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            19471..19704
                     /gene="secG"
                     /locus_tag="PWN45_000640"
     CDS             19471..19704
                     /gene="secG"
                     /locus_tag="PWN45_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL0622429.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            19820..20560
                     /locus_tag="PWN45_000641"
     CDS             19820..20560
                     /locus_tag="PWN45_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDL0622430.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            20594..22966
                     /gene="rnr"
                     /locus_tag="PWN45_000642"
     CDS             20594..22966
                     /gene="rnr"
                     /locus_tag="PWN45_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL0622431.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            22988..23452
                     /gene="smpB"
                     /locus_tag="PWN45_000643"
     CDS             22988..23452
                     /gene="smpB"
                     /locus_tag="PWN45_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL0622432.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            23543..23904
                     /gene="ssrA"
                     /locus_tag="PWN45_000644"
     tmRNA           23543..23904
                     /gene="ssrA"
                     /locus_tag="PWN45_000644"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24774..25097)
                     /locus_tag="PWN45_000645"
     CDS             complement(24774..25097)
                     /locus_tag="PWN45_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622433.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(25113..25366)
                     /locus_tag="PWN45_000646"
                     /pseudo
     CDS             complement(25113..25366)
                     /locus_tag="PWN45_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25451..26179)
                     /locus_tag="PWN45_000647"
     CDS             complement(25451..26179)
                     /locus_tag="PWN45_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDL0622434.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            26615..26770
                     /locus_tag="PWN45_000648"
     CDS             26615..26770
                     /locus_tag="PWN45_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622435.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            26778..27299
                     /locus_tag="PWN45_000649"
                     /pseudo
     CDS             26778..27299
                     /locus_tag="PWN45_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27438..27968
                     /locus_tag="PWN45_000650"
     CDS             27438..27968
                     /locus_tag="PWN45_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0622436.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            28231..31032
                     /gene="clfA"
                     /locus_tag="PWN45_000651"
     CDS             28231..31032
                     /gene="clfA"
                     /locus_tag="PWN45_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDL0622437.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
     gene            31253..32779
                     /gene="vwb"
                     /locus_tag="PWN45_000652"
     CDS             31253..32779
                     /gene="vwb"
                     /locus_tag="PWN45_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HDL0622438.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            33130..34152
                     /gene="emp"
                     /locus_tag="PWN45_000653"
     CDS             33130..34152
                     /gene="emp"
                     /locus_tag="PWN45_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDL0622439.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            34487..34957
                     /locus_tag="PWN45_000654"
     CDS             34487..34957
                     /locus_tag="PWN45_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622440.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            35298..35984
                     /locus_tag="PWN45_000655"
     CDS             35298..35984
                     /locus_tag="PWN45_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL0622441.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            36341..36541
                     /locus_tag="PWN45_000656"
     CDS             36341..36541
                     /locus_tag="PWN45_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0622442.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT        13315 a         5309 c         6792 g        11429 t
ORIGIN      
        1 acattcttat atgaatccat ttgggatgtc gctggattta ttatcttagt taatattcgt
       61 aaacatttaa aattaggaga aacattcttt ttatatttaa cttggtattc aattggtcga
      121 ttctttatag aaggattacg tacagatagc ttaatgctca caagtaatat tagagttgca
      181 caattagtat caattctttt aattttaata agtataagtt taattgtata tagaaggatt
      241 aagtataatc caccgttgta tagcaaagtt ggggcgcttc catggccaac aaaaaaagtg
      301 aagtagtgat agtttgagga aatttttatc aaaaacacat catcatacaa accctttatg
      361 gcgtgtatac cgtcttgtta aattttcgaa agtttttaag aatgtaatta tcattgaatt
      421 ttcgaaattt attccaagta tggtactgaa aagacatata tataaacaac ttttaaatat
      481 taatatcggt aatcaatcgt cgatagctta taaagtaatg ttagatattt tttacccaga
      541 actgattacg attggtagta acagtgttat tggttacaat gtaacaattt tgacgcatga
      601 agcattagtt gatgaatttc gttatggacc agtgacgata ggatctaaca ctttgattgg
      661 tgcaaatgct accattttac ccggtataac gattggtgac aatgtaaaag ttgcagctgg
      721 tacggttgtt tcaaaagata taccggataa tggatttgca tatggcaacc ctatgtatat
      781 aaaaatgatt aggaggtgac aattttatgg cgcaaaagaa taataatgta attccaatga
      841 cttttgatga tgcattttat cgtaaaatgg ctaaacagaa gtttaaacaa agagaatata
      901 aacgagctgc tgaatacttt gaaaaagtgt tagaattgtc acctgatgat atggaaattc
      961 aaattgatta tgcacaatgt ctagtgcaac ttggtattgc taaaaaagca gaacatttat
     1021 tttatgacaa tattatttat aataggcatc tagaagatag cttttatgaa ttgagtcagc
     1081 tcaacattga agttaacgaa ccaaacaagg cattcttgtt tggtattaat tatgttattg
     1141 ttagcgacga ccaagattat agagatgaat tagatcaaat gtttgatgtg aaatatcaaa
     1201 gtgaagaaca aattgaactt gaagctcaat tgtttgtagt tcaaatacta ttccaatatc
     1261 ttttttctca aggtcgatta aaagatgcaa agaattatgt cttacatcaa ccacaagaag
     1321 ttcaagatca tcgtgtagta cgtaatttat tggcaatgtg ttatttatat ctcggtgaat
     1381 atgatacggc taaagcattg tacgaagcac tattacaaga ggatagtaca gatatatatg
     1441 cattatgcca ttatactttg ctactttata acactaagga aaatgaacaa tatcaaaaat
     1501 atttaaaaat attaaacaaa gttgtaccta tgaatgacga tgaaagtttt aaattaggta
     1561 ttgtattaag ttatttaaag cagtatcgtg catcacaaca attgttgtac cctttatata
     1621 aaaaagggaa atttttatca attcaaatgt acaatgcttt agcatataat tattattatt
     1681 taggtgaaga agacgaaagt cattactact gggataaatt gaagcaaatt tctaaagtgg
     1741 aaattggaca tgcgccttgg gtaattgaaa atagcaaaga agtttttgac caacatattt
     1801 tgccattact tcaaagtgat gacagtcatt atcgtttata tggtattttt ttattggatc
     1861 aattaaatgg taaagaaatt gtgatgacgg aaagtatttg gcaggttttg gaaaatctaa
     1921 ataattatga gaaattgtat ttaacgtatt tagttcaagg tttaacgctc aataaattag
     1981 acttcattca tcgcggctta ttaacgcttt accataatga attatttgta agtgaaaatg
     2041 atgtaatggt tgcatggatt aatcaaggtg aactcataat tgctgaaaaa gtagatttaa
     2101 ctgatgttga gccatatatc ggtgcgttta tttatttgta ttttaaaaat caacctcgaa
     2161 acgttacaaa gaagcaaatt acaacatggt taggcataac acaatataaa ctgaacaaaa
     2221 tgattgaatt tctcttgagc atatagattt atgaaaagtt agatttatta tataatgcgc
     2281 ataatgatta ataatgagga ggcgttaata aaatgactga aatagatttt gatatagcaa
     2341 ttatcggtgc aggtccagct ggtatgactg ctgcagtata cgcatcacgt gctaatttaa
     2401 aaacagttat gattgaaaga ggtattccag gcggtcaaat ggctaataca gaagaagtag
     2461 agaacttccc tggtttcgaa atgattacag gtccagattt atctacaaaa atgtttgaac
     2521 acgctaaaaa gtttggtgca gtttatcaat atggagatat taaatctgta gaagataaag
     2581 gcgaatataa agtgattaac tttggtaata aagaattaac agcgaaagcg gttattattg
     2641 ctacaggtgc agaatacaag aaaattggtg ttccgggtga acaagaactt ggtggacgcg
     2701 gtgtaagtta ttgtgcagta tgtgatggtg cattctttaa aaataaacgc ctattcgtta
     2761 tcggtggtgg tgattcagca gtagaagagg gaacattctt aactaaattt gctgacaaag
     2821 taacaatcgt tcaccgtcgt gatgagttac gtgcacagcg tattttacaa gatagagcat
     2881 tcaaaaatga taaaatcgac tttatttgga gtcatacttt gaaatcaatt aatgaaaaag
     2941 acggcaaagt gggttctgtg acattaacgt ctacaaaaga tggttcagaa gaaacacacg
     3001 aggctgatgg tgtattcatc tatattggta tgaaaccatt aacagcgcca tttaaagact
     3061 taggtattac aaatgatgtt ggttatattg taacaaaaga tgatatgaca acatcagtac
     3121 caggtatttt tgcagcagga gatgttcgcg acaaaggttt acgccaaatt gtcactgcta
     3181 ctggcgatgg tagtattgca gcgcaaagtg cagcggaata tattgaacat ttaaacgatc
     3241 aagcttaatt cgaagtcgaa ttaagatgtt gagctgtaaa ttatttggat atttatttta
     3301 atagtgtcat cacagcgtta aaataatgtc ttacttttaa attaaagcaa attatataga
     3361 aaactagaac ttagtacgta tcatttgtgc gtttcaatga gttctagttt ttttatatgt
     3421 tatattaaac ttataacttt atgggagtgg gacagaaatg ataaagagcc actaatgatt
     3481 tattatgtag tggttcttaa acattagcca cagctaatgt gtacttaaaa ataggaatac
     3541 atgagtaaaa ctcatgcata agaaatacta atttctatag aaaaagtatt actttatcgt
     3601 tgtcccaccc caacttgcac attattgtaa gctgactttc cgccagcttc tgtgttgggg
     3661 ccccgccaac ttgcacatta ttgtaagctg acttttcgtc agcttctgtg ttggggcccc
     3721 gccaacttgc attgtctgta gaaattggga atccaatttc tctatgttgg ggcccacacc
     3781 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccacacccc
     3841 aactcgcatt gcctgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga
     3901 gttgaaaaaa gcttgttgca agcgcatttt cattcagtca actactagca atataatatt
     3961 atagacccta ggacattgat ttatgtccca agctcctttt aaatgatgta tatttttaga
     4021 aatttaatct agacatagtt ggaaataaat ataaaacatc gttgcttaat tttgtcatag
     4081 aacatttaaa ttaacatcat gaaattcgtt ttggcggtga aaaaataatg gataataatg
     4141 aaaaagaaaa aagtaaaagt gaactattag ttgtaacagg tttatctggc gcaggtaaat
     4201 ctttggttat tcaatgttta gaagacatgg gatatttttg tgtagataat ctaccaccag
     4261 tgttattgcc taaatttgta gagttgatgg aacaaggaaa tccatcctta agaaaagtgg
     4321 caattgcaat tgatttaaga ggtaaggaac tatttaattc attagttgca gtagtggata
     4381 aagtcaaaag tgaaagtgac gtcatcattg atgttatgtt tttagaagca agtactgaaa
     4441 aattaatttc aagatataag gaaacgcgtc gtgcacatcc tttgatggaa caaggtaaaa
     4501 gatcgttaat caatgcaatt aatgatgagc gagagcattt gtctcaaatt agaagtatag
     4561 ctaattttgt tatagatact acaaagttat cacctaaaga attaaaagaa cgcattcgtc
     4621 gatactatga agatgaagag tttgaaactt ttacaattaa tgtcacaagt ttcggtttta
     4681 aacatgggat tcagatggat gcagatttag tatttgatgt acgattttta ccaaatccat
     4741 attatgtagt agatttaaga cctttaacag gattagataa agacgtttat aattatgtta
     4801 tgaaatggaa agagacggag attttctttg aaaaattaac tgatttgtta gattttatga
     4861 tacccgggta taaaaaagaa gggaaatctc aattagtaat tgccatcggt tgtacgggtg
     4921 gacaacatcg atctgtagca ttagcagaac gactaggtaa ttatctaaat gaagtatttg
     4981 aatataatgt ttatgtgcat catagggacg cacatattga aagtggcgag aaaaaatgag
     5041 acaaataaaa gttgtactta tcggtggtgg cactggctta tcagttatgg ctaggggatt
     5101 aagagaattc ccaattgata ttacggcgat tgtaacagtt gctgataatg gtgggagtac
     5161 agggaaaatc agagatgaaa tggatatacc agcaccagga gacatcagaa atgtgattgc
     5221 agctttaagt gattctgagt cagttttaag ccaacttttt cagtatcgct ttgaagaaaa
     5281 tcaaattagc ggtcactcat taggtaattt attaatcgca ggtatgacta atattacgaa
     5341 tgatttcgga catgccatta aagcattaag taaaatttta aatattaaag gtagagtcat
     5401 tccatctaca aatacaagtg tgcaattaaa tgctgttatg gaagatggag aaattgtttt
     5461 tggagaaaca aatattccta aaaaacataa aaaaattgat cgtgtgtttt tagaacctaa
     5521 cgatgtgcaa ccaatggaag aagcaatcga tgctttaagg gaagcagatt taatcgttct
     5581 tggaccaggg tcattatata cgagcgttat ttctaactta tgtgtgaatg gtatttcaga
     5641 tgcgttaatt cattctgatg cgcctaagct atatgtttct aatgtgatga cgcaacctgg
     5701 ggaaacagat ggttatagcg tgaaagatca tatcgatgcg attcatagac aagctggaca
     5761 accgtttatt gattatgtca tttgtagtac acaaactttc aatgctcaag ttttgaaaaa
     5821 atatgaagaa aaacattcta aaccagttga agttaataag gctgaacttg aaaaagaaag
     5881 cataaatgta aaaacatctt caaatttagt tgaaatttct gaaaatcatt tagtaagaca
     5941 taatactaaa gtgttatcga caatgattta tgacatagct ttagaattaa ttagtactat
     6001 tcctttcgta ccaagtgata aacgtaaata atatagaacg taatcatatt atgatatgat
     6061 aatagagctg tgaaaaaaat gaaaatagac agtggttcta aggtgaatca tgttttaaat
     6121 aagaaaggaa tgactgtacg atgagctttg catcagaaat gaaaaatgaa ttaactagaa
     6181 tagacgtcga ggaaatgaat gcaaaagcag agctcagtgc actgattcga atgaatggtg
     6241 cacttagtct ttcaaatcaa caatttgtta taaatgttca aacggaaaat gcaacaacgg
     6301 caagacgtat ttattcgttg attaaacgtg tctttaatgt ggaagttgaa atattagtcc
     6361 gtaaaaaaat gaaacttaaa aaaaataata tttatatttg tcgtacaaag atgaaagcga
     6421 aagaaattct tgatgaatta ggaattttaa aagacggcat ttttacgcat gaaattgatc
     6481 attcaatgat tcaagatgac gaaatgagac gcagttactt gagaggagct tttctggcag
     6541 gtggctcagt gaataaccct gaaacatctt cgtaccattt ggaaattttt tctcaaaatg
     6601 agagtcatgc agaaggctta acgaaactaa tgaatagtta tgagttgaat gccaaacatt
     6661 tagagcgaaa aaaaggaagt attacgtatt taaaagaagc ggaaaagatt tcggattttc
     6721 ttagtttgat aggtggctat caagcgttat taaaatttga agacgtacgt attgtaagag
     6781 atatgcgtaa ttctgttaac cgactcgtta attgtgaaac ggccaatcta aataaaacag
     6841 ttagtgctgc gatgaaacaa gttgagagca ttaaattgat tgataaagaa attggtattg
     6901 aaaatttacc agacaggttg agagagattg ctagaattcg agtagaacat caagaaattt
     6961 cgttgaaaga gcttggagaa atggtatcaa ctggtccaat ttcaaaatca ggtgtaaatc
     7021 accgattaag aaaactgaat gatttagccg ataagattag aaatggtgaa caaatagaat
     7081 tataagtaag aaggtgtttt tggtagtgaa ttatcaaaaa cacttttttt atgattaaaa
     7141 agtgtttaat gaataatagt cgactaacta ttaaattgag ctggaaagtt taatgaagga
     7201 aattcaataa ccaagtgatg cctaattttc tcattaaata gcatcatatg tatcaatttg
     7261 gtcataagaa aatatgaatg tgataattaa ttgagtccct gaaagtccct gatcatagac
     7321 aaaaacaaaa tcagcataaa taagaatccc gacattgcgg gattctctgt attgaaaagt
     7381 attgtatttt attaaaacag cctccttgaa gggaattgaa cccctatctt aagaaccgga
     7441 atcttacgtg ttatccatta caccacaagg agcaaatatg taatcatatc tctgtactat
     7501 gagaagtaaa ataagtacaa tctaaaatag atattatcag tttacaaagg aaagagaaaa
     7561 gcgtcaaaca atgtaactat ttaaagtcaa agtgtttgac caaatttgac ttaatatgta
     7621 aaataatgag taacagttat tacaaggagg aaatatagat gaatttaatt cctacagtta
     7681 ttgaaacaac aaaccgcggt gaacgtgcat atgatatata ctcacgttta ttaaaagacc
     7741 gtattattat gttaggttca caaattgatg acaacgtagc aaattcaatc gtatcacagt
     7801 tattattctt acaagcgcaa gactcagaga aagatattta tttatacatt aattcaccag
     7861 gtggaagtgt aacagctggt tttgcgattt atgatacaat tcaacacatt aaacctgatg
     7921 ttcaaacaat ttgtatcggt atggctgcat caatgggatc attcttatta gcagctggtg
     7981 caaaaggtaa acgtttcgcg ttaccaaatg cagaagtaat gattcaccaa ccattaggtg
     8041 gtgctcaagg acaagcaact gaaatcgaaa ttgctgcaaa tcacatttta aaaacacgtg
     8101 aaaaattaaa ccgcatttta tcagagcgta ctggtcaaag tattgaaaaa atacaaaaag
     8161 acacagatcg tgataacttc ttaactgcag aagaagctaa agaatatggc ttaattgatg
     8221 aagtgatggt acctgaaaca aaataattca aagtaaagag tagactaagc tgtctgctct
     8281 ttttgtatga gtaaaccaag gtgtcaataa tttgtttact atactttgag cggaaatatg
     8341 attgaatgaa gctagttgaa ccgtaactat atgaaatgtt cccttcaaag tagacattga
     8401 aaggaacatt tcaatccttt gtttgtaagt cgctctagac attacattta gtacatatgt
     8461 tgtttctaat gctcattaat ggtattgatt attctttaat taaatcttca agtgccattt
     8521 ttaaattact atatttaaat tggaatccca atgcttgaat tttattaggt aatacttttt
     8581 gagtatccaa tactactgtt gacatttgac caagtatgag acgcattgca agacttggtg
     8641 cccaagtttc atgaggctta tgcatagctc ttgctaaagt gtagccaaat aaattttgac
     8701 gttcaggtat aggtgcagtt aaattaaacg gaccactagc tgactcgtta tttattaaaa
     8761 ataaaatagc ttgaattaaa tcattgatat gaatccatga ataccattgt tgaccagaac
     8821 ctaatttacc accaatgtaa tattcgtatg gtagtttcat tgtttgtaac gcaccgcctt
     8881 cattcgataa aattataccg aaacgaccga tgacaactcg tgtacctaat tgttcaaatt
     8941 gttgtgcgaa acgttcccat tgatacacaa tatctgataa gaaatcaaaa ggtaaagttt
     9001 tataaacttc tgtgtaactc ataaataaat caggaggata gtaaccagtg gcactagcat
     9061 taaataaagc tttaggtgct ttattacgtg atttaaacaa ttcatataaa gcttgcgtag
     9121 attgaattct acttagcatt agcgtttgtt tatattccgg tgtccatcgt ttattcaatg
     9181 tagcacctgc taagttgatg accacatcga tattttgagg aactttgtgt tcccacccag
     9241 atttagccca gttgacatat gaaattttct tatcatttga aatttggtcg tgtcgcgtta
     9301 atatcgtgat atgtgaatct gattttttaa tttcattaac taattgagat ccaaccatac
     9361 cagtcccacc agtaattaag tattgtttca ttatcattca ccccatgtaa ttttgtattt
     9421 agttactatt taataatcat tattgttgtt caaaggttat acattattta gacaataata
     9481 tgtcaataac tttttttgaa ttgtatttta ataaaaatga tataagttat aataaaggtg
     9541 taccttcgat aacgaataaa catctcttaa aagtatgtgt aaaacgctgc atgatacaaa
     9601 cgaaggtaaa aatttgactc cctttagtag tggacccgta cgttaatcgt gcgggtcgtt
     9661 tttatttatt tcttattccc attatacatc aatttaaagc attaattttt taaacaaatt
     9721 taagaataca tagtaatata acaatctaaa cataaaaact tttaacacaa cacttaaacc
     9781 aatgctttaa ttttcaatac gtagctataa tttgttgtaa aatcaaaaag gttaaaatgt
     9841 taattttcaa aaaaggctca aaatatgttt gatttagtta ttaaatgtta agatatataa
     9901 gactactatt tctttgtaaa aatgaatccg atttacgagt gagtaatagt gaaggcagtt
     9961 ttaagttgaa gaaggcaaaa agagtaaatg tttattaata tttgtagaaa ctaggtaagc
    10021 aaattagttg tgaaaatgtt aatggttgcg tgataatttc tatatttaaa ttagtttgaa
    10081 gtgagggaga gtatgtcgaa tcaaaattac gactacaata aaaatgaaga tggaagtaag
    10141 aagaaaatga gtacaacagc gaaagtagtt agcattgcga cggtattgct attactcgga
    10201 ggattagtat ttgcaatttt tgcatatgta gatcattcga ataaagctaa agaacgtatg
    10261 ttgaacgaac aaaagcagga acaaaaagaa aagcgtcaaa aagaaaatgc agaaaaagag
    10321 agaaagaaaa agcaacaaga ggaaaaagag cagaatgagc tagattcaca agcaaaccaa
    10381 tatcagcaat tgccacagca gaatcaatat caatatgtgc cacctcagca acaagcacct
    10441 acaaagcaac gtcctgctaa agaagagaat gatgataaag catcaaagga tgagtcgaaa
    10501 gataaggatg acaaagcatc tcaagataaa tcagatgata atcagaagaa aactgatgat
    10561 aataaacaac cagctcagcc taaaccacag ccgcaacaac caacaccaaa gccaaataat
    10621 aatcaacaaa acaatcaatc aaatcagcaa gcaaaaccac aagcaccaca acaaaatagc
    10681 caatcaacaa caaataaaca aaataatgct aatgataagt agtatttagt caaacaaaaa
    10741 tgaaccagta tgacagacaa cacaattaat taggttgtct cgaatattgg ttcttatttt
    10801 tataattgtt aattagggga gagatgatac ttaaatagtt agttatttat tttacggata
    10861 gtgaaattta ttttgagtga ggtgggacag aaatgatatt ttcgcaaaat ttatttcgtc
    10921 gtcccacccc aactcgcatt gcctgtagaa tttcatttcg aaattctcta tgttggggcc
    10981 cctgacttta attgaaaaaa gcttgttaca agtgcatttt cgttcggtta actactacta
    11041 atgtgacttt ttggattcta gagcattgat ttatgtccta gtctcaaata ataagcaaat
    11101 gattatcgag tcagtataag ggtatacatt tgactacagc gaataaaata aaacgttttg
    11161 ttgaataaca aatcgaaaat atattgcaag cgctttatca aattatttag aaaatttaag
    11221 ttttatgctt gcaatttttg aaatagaata gtactattgc aagtgtaaag aggttaattt
    11281 ttgtcccacg cgggacttaa aaaggcaacc actggttgtg acatatcctt atttacattt
    11341 ataaatataa ggaggaggta gtagtgaaag acttattgca agcacagcaa aagcttatac
    11401 cggatctcat agataaaatg tataaacgtt tttctattct tactactatc tcaaaaaatc
    11461 agcctgtcgg acgtcgaagt ttaagcgaac atatggatat gactgaacgt gtactgcgtt
    11521 ctgaaacaga tatgcttaag aaacaagatt tgataaaagt taagcctacc ggaatggaaa
    11581 ttacagctga aggtgagcaa ctgatttcgc aattgaaagg ttactttgat atctatgcag
    11641 atgataatcg tctgtcagaa ggtattaaga ataaatttca aattaaggaa gttcatgttg
    11701 ttcctggtga tgctgataat agtcaatctg ttaaaacaga attaggtaga caagcaggtc
    11761 aattacttga aggcatatta caagaagacg cgatagttgc tgtaactggc ggatccacga
    11821 tggcatgtgt tagtgaagca attcatttat taccatataa tgtattcttc gtaccagcca
    11881 gaggtggact aggcgaaaat gttgtctttc aggcaaacac aattgcagcc agtatggcac
    11941 aacaagctgg cggttattat acgacgatgt atgtacctga taatgtcagt gaaacaacat
    12001 ataatacatt gttgttagag ccatcagtca taaacacttt agacaaaatt aaacaagcaa
    12061 acgttatatt acacggcatt ggtgatgcgc tgaagatggc gcatcgacgt caatcacctg
    12121 aaaaggtcat tgaacaactt caacatcatc aagctgtcgg agaggcattt ggttattatt
    12181 ttgatacaca aggtcaaatt gtccataagg ttaaaacaat tggacttcaa ttagaagacc
    12241 ttgaatcaaa agactttatt tttgcagttg caggaggcaa atcgaaaggt gaagcaatta
    12301 aagcatactt gacgattgca cccaagaata cagtgttaat cactgatgaa gccgcagcaa
    12361 agataatact tgaataagag ataaaaagtt taatactttt taaatatcat tttaaaggag
    12421 gccattataa tggcagtaaa agtagcaatt aatggttttg gtagaattgg tcgtttagca
    12481 ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag cagtaaacga cttaacagat
    12541 gacgacatgt tagcgcattt attaaaatat gacactatgc aaggtcgttt cacaggtgaa
    12601 gtagaggtag ttgatggtgg tttccgcgta aatggtaaag aagttaaatc attcagtgaa
    12661 ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg atgtagtatt agaatgtact
    12721 ggtttctaca ctgataaaga taaagcacaa gctcatattg aagcaggcgc taaaaaagta
    12781 ttaatctcag caccagctac tggtgactta aaaacaatcg tattcaacac taaccaccaa
    12841 gagttagacg gttctgaaac agttgtttca ggtgcttcat gtactacaaa ctcattagca
    12901 ccagttgcta aagttttaaa cgatgacttt ggtttagttg aaggtttaat gactacaatt
    12961 cacgcttaca caggtgatca aaatacacaa gacgcacctc acagaaaagg tgacaaacgt
    13021 cgtgctcgtg cagcggcaga aaacatcatc cctaactcaa caggtgctgc taaagctatc
    13081 ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg gtgcacaacg tgttcctgta
    13141 gctacaggtt cattaactga attaacagta gtattagaaa aacaagacgt aacagttgaa
    13201 caagttaacg aagctatgaa aaatgcttca aacgaatcat tcggttacac tgaagacgaa
    13261 atcgtttctt cagacgttgt aggtatgact tacggttcat tattcgacgc tacacaaact
    13321 cgtgtaatgt cagttggcga ccgtcaatta gttaaagttg cagcttggta tgataacgaa
    13381 atgtcatata ctgcacaatt agttcgtaca ttagcatact tagctgaact ttctaaataa
    13441 ttttagtata gtttttattc aaatacgcta gtgctcagaa ctatttagca ttaattaaag
    13501 cttatgagta agcggggagc acaaacgctt ctccgcttat ttttatataa aatttcctaa
    13561 ttacaaggag gaaacaccat ggctaaaaaa attgtttctg atttagatct taaaggtaaa
    13621 acagtcctag tacgtgctga ttttaacgta cctttaaaag acggtgaaat tactaatgac
    13681 aaccgtatcg ttcaagcttt acctacaatt caatacatca tcgaacaagg tggtaaaatc
    13741 gtactatttt cacatttagg taaagtgaaa gaagaaagtg ataaagcaaa attaacttta
    13801 cgtccagttg ctgaagactt atctaagaaa ttagataaag aagttgtttt cgtaccagaa
    13861 acacgcggcg aaaaacttga agctgctatt aaagacctta aagaaggcga cgtattatta
    13921 gttgaaaata cacgttatga agatttagac ggtaaaaaag aatctaaaaa tgatccagaa
    13981 ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa atgatgcttt tggtactgcg
    14041 catcgtgagc atgcatctaa tgttggtatt tctacacatt tagaaactgc agctggattc
    14101 ttaatggata aagaaattaa gtttattggc ggcgtagtta acgatccaca taaaccagtt
    14161 gttgctattt taggtggagc aaaagtatct gacaaaatta atgtcatcaa aaacttagtt
    14221 aacatagctg ataaaattat catcggcgga ggtatggctt atactttctt aaaagcgcaa
    14281 ggtaaagaaa ttggtatttc attattagaa gaagataaaa tcgacttcgc aaaagattta
    14341 ttagaaaaac atggtgataa aattgtatta ccagtagaca ctaaagttgc taaagaattt
    14401 tctaatgatg ccaaaatcac tgtagtacca tctgattcaa ttccagcaga ccaagaaggt
    14461 atggatattg gaccaaacac tgtaaaatta tttgcagatg aattagaagg tgcgcacact
    14521 gttgtatgga atggacctat gggtgtattc gagttcagta actttgcaca aggtacaatt
    14581 ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta cgattatcgg tggcggtgat
    14641 tcagctgcag cagcaatctc tttaggtttt gaaaatgact tcactcatat ttcaactggt
    14701 ggcggcgcgt cattagagta cctagaaggt aaagaattgc ctggtatcaa agcaatcaat
    14761 aataaataat aaagtgatag tttaaagtga tgtggcatgt ttgtttaaca ttgttacggg
    14821 aaaacagtca caagatgaca tcgtgtttca tcacttttca aaaatattta caaaacaagg
    14881 agtgtcttta atgagaacac caattatagc tggtaactgg aaaatgaaca aaacagtaca
    14941 agaagcaaaa gacttcgtca atgcattacc aacattacca gattcaaaag aagtagaatc
    15001 agtaatttgt gcaccagcaa ttcaattaga tgcattaact actgcagtta aagaaggaaa
    15061 agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc gaagataatg gtgcgttcac
    15121 aggtgaaacg tctccagttg cattagcaga tttaggcgtt aaatacgttg ttatcggtca
    15181 ttctgaacgt cgtgaattat tccacgaaac agatgaagaa attaacaaaa aagcgcacgc
    15241 tattttcaaa catggaatga ctccaattat atgtgttggt gaaacagacg aagagcgtga
    15301 aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag aaagctgttg caggtttatc
    15361 tgaagatcaa cttaaatcag ttgtaattgc ttatgaacca atctgggcaa tcggaactgg
    15421 taaatcatca acatctgaag atgcaaatga aatgtgtgca tttgtacgtc aaactattgc
    15481 tgacttatca agcaaagaag tatcagaagc aactcgtatt caatatggtg gtagtgttaa
    15541 acctaacaac attaaagaat acatggcaca aactgatatt gatggggcat tagtaggtgg
    15601 cgcatcactt aaagttgaag atttcgtaca attgttagaa ggtgcaaaat aatcatggct
    15661 aagaaaccaa ctgcgttaat tattttagat ggttttgcga accgcgaaag cgaacatggt
    15721 aatgcggtaa aattagcaaa caagcctaat tttgatcgtt attacaacaa atatccaacg
    15781 actcaaatcg aagcgagtgg cttagatgtt ggactacctg aaggacaaat gggtaactca
    15841 gaagttggtc atatgaatat cggtgcagga cgtatcgttt atcaaagttt aactcgaatc
    15901 aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg ttttaaataa tgcaattgca
    15961 cacgtgaatt cacatgattc agcgttacac atctttggtt tattgtctga cggtggtgta
    16021 cacagtcatt acaaacattt atttgctttg ttagaacttg ctaaaaaaca aggtgttgaa
    16081 aaagtttacg tacacgcatt tttagatggc cgtgacgtag atcaaaaatc cgctttgaaa
    16141 tacatcgaag agactgaagc taaattcaat gaattaggca ttggtcaatt tgcatctgtg
    16201 tctggtcgtt attatgcaat ggatcgtgac aaacgttggg aacgtgaaga aaaagcttac
    16261 aatgctattc gtaattttga tgccccaact tatgcaactg ccaaagaagg tgtagaagca
    16321 agctataatg agggcttaac tgacgaattc gtagtaccat tcatcgttga gaatcaaaat
    16381 gacggtgtta atgatggaga tgcagtgatc ttctataatt tccgacctga tagagcagcg
    16441 caattatcgg aaatttttgc gaacagagca ttcgaaggct ttaaagttga acaagttaaa
    16501 gacttattct atgcaacatt cactaagtat aatgacaata tcgatgcggc tatcgtcttc
    16561 gaaaaagttg atttaaataa tacaattggt gaaattgcac aaaataacaa tttaactcaa
    16621 ttacgtattg cagaaactga aaaataccct cacgttactt actttatgag tggtggacgt
    16681 aacgaggaat ttaaaggtga acgccgtcgt ttaattgatt cacctaaagt tgcaacgtat
    16741 gacttgaaac cagaaatgag tgcttatgaa gttaaagatg cattattaga agagttaaat
    16801 aaaggtgact tggacttaat tattttaaac tttgctaacc ctgatatggt tggacatagt
    16861 ggtatgcttg agccgacaat caaagcaatc gaagcggttg atgaatgttt aggagaagtg
    16921 gttgataaga ttttagacat ggacggttat gcaattatta ctgctgacca tggtaactct
    16981 gatcaagtat tgacggatga tgatcaacca atgactacgc atacaacgaa cccagtacca
    17041 gtgattgtaa caaaagaagg cgttacactt agagaaactg gtcgcttagg tgacttagca
    17101 cctacattat tagatttatt aaatgtagaa caacctgaag atatgacagg tgaatcttta
    17161 attaaacact aatattgtaa aagatgttaa gtaaacgctt aatgacactt attttttgaa
    17221 aataatagta atatcatttt gttaaatgaa agaataaagc tataataatt atagaataac
    17281 tatttaaagg agattataaa catgccaatt attacagatg tttacgctcg cgaagtctta
    17341 gactctcgtg gtaacccaac tgttgaagta gaagtattaa ctgaaagtgg cgcatttggt
    17401 cgtgcattag taccatcagg tgcttcaact ggtgaacacg aagctgttga attacgtgat
    17461 ggagacaaat cacgttattt aggtaaaggt gttactaaag cagttgaaaa cgttaatgaa
    17521 atcatcgcac cagaaattat tgaaggtgaa ttttcagtat tagatcaagt atctattgat
    17581 aaaatgatga tcgcattaga cggtactcca aacaaaggta aattaggtgc aaatgctatt
    17641 ttaggtgtat ctatcgcagt agcacgtgca gcagctgact tattaggtca accactttac
    17701 aaatatttag gtggatttaa tggtaagcag ttaccagtac caatgatgaa catcgttaat
    17761 ggtggttctc actcagatgc tccaattgca ttccaagaat tcatgatttt acctgtaggt
    17821 gctacaacgt tcaaagaatc attacgttgg ggtactgaaa ttttccacaa cttaaaatca
    17881 attttaagca aacgtggttt agaaactgca gtaggtgacg aaggtggttt cgctcctaaa
    17941 tttgaaggta ctgaagatgc tgttgaaaca attatccaag caatcgaagc agctggttac
    18001 aaaccaggtg aagaagtatt cttaggattt gactgtgcat catcagaatt ctatgaaaat
    18061 ggtgtatatg actacagtaa gttcgaaggc gaacacggtg caaaacgtac agctgcagaa
    18121 caagttgact acttagaaca attagtagac aaatatccta tcattacaat tgaagacggt
    18181 atggacgaaa acgactggga tggttggaaa caacttacag aacgtatcgg tgaccgtgta
    18241 caattagtag gtgacgattt attcgtaaca aacactgaaa ttttagcaaa aggtattgaa
    18301 aacggaattg gtaactcaat cttaattaaa gttaaccaaa tcggtacatt aactgaaaca
    18361 tttgatgcaa tcgaaatggc tcaaaaagct ggttacacag cagtagtttc tcaccgttca
    18421 ggtgaaacag aagatacaac aattgctgat attgctgttg ctacaaacgc tggtcaaatt
    18481 aaaactggtt cattatcacg tactgaccgt attgctaaat acaatcaatt attacgtatc
    18541 gaagatgaat tatttgaaac tgctaaatat gacggtatca aatcattcta taacttagat
    18601 aaataatttt ctttataatc aaatgctgac ataattttag ttgaggatta ttatgacggt
    18661 ataaattaaa taaagatttt gagttcacgc ttaaataagt tcacgcttaa atttatagcc
    18721 tgccacagag ttgagactgt ggtaggtttt ttattttgaa gtattaatca taacagacta
    18781 ataatcatga ggtaactaat aacacatatt taacttgtat tcttaaactg gtataataaa
    18841 tttatgttga aatgaatatt gtatgacagg gtattcactt ttattaaaag gtaaaattaa
    18901 ataaaggttt tatagaacgt atttaaatat atgaggagta aacaaatggc tgatagaacg
    18961 aataaagaaa ttaaaacagg acgctttatt gcaactgcat caatcgtatt ctcaatatta
    19021 ttgattattc attactttgt ttcgttggat aatgcgactg ccaaagcatt acttaattta
    19081 acgaatcaaa acacttcaga taaagcgatt gattacattt taaacagctt tagattcact
    19141 ggtattatgt atattttggc ttatctagca ggcttcatca ctttttggaa tcgacatact
    19201 tatgtgtggt ggtttatgtt tgcagtttat gtatcaaata gtttgtttac gttgattaat
    19261 ttatcaatca caattcaagc aataaaagct gcacacggtg cgtacttaac attgccaatt
    19321 ttaattgtta ttataggttc ggttgcatta gcgatttata tgcttgttgt ttctatcaaa
    19381 cgtaaaagta catttaatcg ctagaaaatt gattttaaca ataaaaatat gatatactac
    19441 ttgtcgtata taaggaacgg aggacaattt atgcatacat ttttaatcgt attattaatc
    19501 attgattgta ttgcattaat aactgttgta ctactccaag aaggtaaaag cagtggactt
    19561 tcaggtgcca tcagtggtgg tgctgagcag ttattcggta aacaaaaaca acgtggcgtc
    19621 gatttattct taaatagatt aacaattatt ttatcaatat tattttttgt acttatgatt
    19681 tgcataagtt atcttggtat gtaaggtccg gcgatgtaaa tgtcgggctt ttttatttat
    19741 aattaagaat gtaatagttt aacaataagc tatgtaaaat atatagccta gttaagtatg
    19801 caaagggagc gttagattta tgcagataaa attaccaaaa cctttctttt ttgaggaagg
    19861 taaacgtgcc gtgttattac tacatggttt tacaggcaat tcgtctgatg ttcgtcaatt
    19921 aggtcgattt ttacaaaaga aaggttatac atcatatgca ccgcaatatg aaggccacgc
    19981 ggcaccacca gatgaaatac tgaaatctag tcctttcgtt tggtttaaag atgcgttaga
    20041 tggttatgat tatcttgttg aacaaggtta tgatgaaatt gttgttgctg gtctatcatt
    20101 aggtggggat tttgctttaa aattaagctt aaatagagat gtaaagggta ttgtaacgat
    20161 gtgtgctcct atgggtggca aaactgaagg tgccatttat gaaggctttt tagaatatgc
    20221 acgcaatttt aaaaagtatg aaggtaaaga tcaagagact attgataatg aaatggatca
    20281 ttttaaacca actgaaactt taaaagaact aagtgaagca ttagatacga ttaaagagca
    20341 agttgatgaa gtgttggatc ctattttagt gattcaagca gaaaacgaca atatgattga
    20401 tccacaatcc gcaaattata tatatgacca tgtagattct gatgacaaaa atatcaagtg
    20461 gtacagtgaa tctggacatg ttattacgat tgataaagag aaagaacaag tatttgaaga
    20521 tatttatcaa tttttagagt cattagactg gtcagaataa aaagagattt taacattaga
    20581 aaggaggggc ataatgaatt taaagcaatc tatagaagag attattaatc aacctgaata
    20641 tgaacctatg tcagtgtcag attttcaaga tgcattaggt ttaagcagtg ccgactcgtt
    20701 tagagattta attaaggtgc ttgtggagtt agaacaatca ggattaatcg aacgtacaaa
    20761 aacagacaga taccaaaaaa agcatagtta tagaggtcaa tcaaaattga taaaaggaac
    20821 gttaagtcaa aataaaaaag gctttgcatt cttaagacct gaagatgagg atatggaaga
    20881 tatatttatt cccccgacga aaattaatcg tgccttggat ggagatactg ttattgtaga
    20941 aatccatcaa tcaaaaggtg aacataaagg taaaatcgaa ggggaagtta agtcgattga
    21001 gaagcattct gtaactcaag ttgttggtac gtatagtgaa gctagacatt ttggctttgt
    21061 tattccggat gataaacgta ttatgcaaga tattttcatt cctaaaggtc aaagtttagg
    21121 cgcagtcgat ggtcataagg tacttgtaca aattactaag tatgctgatg gttcagataa
    21181 tccagaagga catatttctg ctattttagg acataaaaat gatcctggcg tagatatttt
    21241 atctattatc tatcaacatg gcatagaaat tgaatttcct gatgaagtgt tacaagaagc
    21301 tgaagcagta cctgatcata ttgaaaatac tgaaattaaa ggccgtcatg atttacgtga
    21361 tgaattgaca atcacaattg atggtgctga tgctaaagac ttagatgacg caattagtgt
    21421 taaaaagtta gcgaacggta atacgcaatt aactgtaagt attgctgatg tcagctatta
    21481 tgtaacagaa ggttctgcat tggataaaga ggcatatgat agagcgacaa gtgtatatct
    21541 tgttgaccgt gtaattccaa tgattccaca tcgattaagt aatggtattt gttcattgaa
    21601 tcctaatgtt gatcgtttaa ctctaagctg tcgcatggaa atcgatgcta gtggtcgcgt
    21661 tgttaaacat gaaatttttg atagtgttat acattctgat tatcgaatga cgtatgatgc
    21721 ggtaaatcag attattactg aaaaggatcc taacattcgc gaacaatata atgaaattac
    21781 gcctatgcta gatttagcac aagatttatc taatcgtttg attcaaatga gaaaacgacg
    21841 tggtgaaatc gattttgata ttagtgaagc aaaagtatta gttaacgaag acggtatacc
    21901 aacagatgtt caattaagac aacgtggcga gggtgaacgt ctaattgaat catttatgtt
    21961 aattgcaaat gaaacagttg ctgaacattt tagtaagtta gatgtacctt ttatttaccg
    22021 agtgcatgag caacctaaat cagatcgctt aagacaattc tttgatttta ttacaaactt
    22081 tggcatcatg attaagggta ctggcgaaga tattcatcca acaacacttc aaaaggttca
    22141 agaagaagta gaaggtcgac ctgaacaaat ggtcatttca acaatgatgt tgcgttcaat
    22201 gcaacaagcg cattatgatg atgtgaactt gggacatttt ggcttatcag ctgaatatta
    22261 tacgcatttt acatcaccaa ttagacgtta tcctgattta acagttcatc gtttaatccg
    22321 taagtattta attgagaaat caatggataa caaagaagtg aagcgttggg aagacaaatt
    22381 gcctgagtta gctgaacata cttctaaacg tgaacgtcgt gctattgagg cagaacgtga
    22441 tactgatgaa ttgaaaaaag cagaatatat gattcaacat attggtgatg aatttgaagg
    22501 tattgtcagc tcagtagcta acttcggtat gttcattgaa ttgccaaata cgatagaagg
    22561 tatggttcat attgcgaata tgactgatga ttattaccgt tttgaagagc gtcaaatggc
    22621 attaattggt gagcgtcaag ctaaagtatt tagaattggt gacacagtta aggttaaagt
    22681 gacgcatgtt gatgtagatg aacgattaat tgattttcaa attgtaggta tgcctttacc
    22741 gaaaaatgat cgatcacagc gcccagcgcg aggtaagaca attcaagcca aaacgcgtgg
    22801 taaatcatta gataaatcaa aatctgatga taagggtcgt aagaaaaaag gtaagcaacg
    22861 taaaggtaaa aaccaacgta ataatgataa atcaggtaat agtaagcata agccatttta
    22921 taaagataaa agtgtgaaaa agaaagcacg tcgtaagaaa aaataagcag caatgaggtg
    22981 agtatgaatg gctaagaaga aatcaccagg tacattagcg gaaaatcgta aggcaagaca
    23041 tgattataat attgaagata cgattgaagc gggaattgta ttgcaaggca cagaaataaa
    23101 atcaattcgc cgaggtagtg ctaaccttaa agatagttat gcgcaagtta aaaacggtga
    23161 aatgtatttg aataatatgc atatagcacc atacgaagaa gggaatcgtt ttaatcacga
    23221 tcctcttcgt tctcgaaaat tattattgca caagcgtgaa atcattaaat tgggtgatca
    23281 aacacgtgag attggttatt cgattgtgcc gttaaagctt tatttgaagc atggacattg
    23341 taaagtatta cttggtgttg cacgaggtaa gaaaaaatat gataaacgtc aagctttgaa
    23401 agaaaaagca gtcaaacgag atgttgcgcg cgatatgaaa gcccgttatt aagcgattta
    23461 gttgcttaat cgggctatat ttgatatagt tatatgtgct tttgtaaatt acaaaagtat
    23521 gatttgtttg atttattatt tcggggacgt tcatggattc gacaggggtc ccccgagctc
    23581 attaagcgtg tcggagggtt gtcttcgtca tcaacacaca cagtttataa taactggcaa
    23641 atcaaacaat aatttcgcag tagctgccta atcgcactct gcatcgccta acagcatttc
    23701 ctatgtgctg ttaacgcgat tcaaccttaa taggatatgc taaacactgc cgtttgaagt
    23761 ctgtttagaa gaaacttaat caaactagca tcatgttggt tgtttatcac ttttcatgat
    23821 gcgaaaccta tcgataaact acacacgtag aaagatgtgt atcaggacct ttggacgcgg
    23881 gttcaaatcc cgccgtctcc atatttgtag cctacagcct ttgtggttgt gggctttttt
    23941 attttgtgtt tttcagggga taatgcattg cagaatttgt tgtgagtatt gatatagcag
    24001 tgtttgtata ggtgtttatt tgatggagga aagagtaata agtgattatg aattagtttt
    24061 tgagatataa ggggacagtg atgtgtgtca aataagtgtc aaaaaagttg gattctgagt
    24121 tttacattca acattgttca tgaagaaact tctttatacg caaaaaattc tccatgttat
    24181 atatgtcaat ataaaaatgt gaatcgtcta cacttaattg gataaatggc tactgaaaaa
    24241 gaacttttca tttttgttac gtcactaagt gggtgtagtt ataaagagat gagccgagtt
    24301 ttgatatttt cattagaatc aatatgccta ttaacacaat cagcaatagt tgacgagacg
    24361 gaaataaaag aagtcgtagt taagaaatgc atttcacaac ataccattgt agccattttt
    24421 attgttttgg atgataaact ctttttggaa tttttagttt ttataatttg caactacact
    24481 acttctttta ctaatattaa tgtctaagta atcgataaaa aattttccat tgaataaatg
    24541 agaagttaaa aactttactt aacctttctc attgcatttt cctattcacg attttaagaa
    24601 cccaacatac tacaaacgaa ttttaaaagg cgagagtaaa gcttacttgt ttattataca
    24661 tatttaaaat ccaagagtca gaacagacta ctcctcttta taactataaa aaatagctat
    24721 gaaaaaatct atcgtcatag attccttcat agctaatctt agtatgttta tttttatttt
    24781 aggatgctat ttatcaactc aacatataac tcactatttt tataaccttc taatatatca
    24841 ttaacttgtc taataggtat ttctggtact tctctaatgt tttccaattt tgttttaaat
    24901 tgtttttttg ttatttgctc tttatttgta gccaattgga acaagtaaga atctagcata
    24961 ttaatttctt tatatgaata catatatctt aataacacta aatctctagt ttttaagtta
    25021 ggcgctagtt cttcttgtaa ttgttctatt gattgtttca ttaataacaa tctcatttct
    25081 aattcttcat tattcatttt atcacactct ttttatatta atgcttgacc aacttgggaa
    25141 acccaaaacc ctatgcttct tgcagtagaa tctttaatac cagttcccat caatgcttgt
    25201 gaaacttgac cttgtacatt tccccatgta gcctcttctt gttttaatgc attattcaat
    25261 gcgggattta caaatttatc ccatcttttt tttatgattt tccggcacgg ggactgattt
    25321 ctttaacacc attaaacaca gattttttat ttttaatcat agctttatag tatcatgttg
    25381 gctaagctat aaataagtca gtttctctaa aaattaaata actgaatgta agacaatcaa
    25441 caaaccaaat ttatacttca tctaaaccac tgtggtcgtc atctttttgc ttttcttttt
    25501 ctttctctcg ttcttgttct tttttgtact cttcttcaaa ttctttttct ttcttttcta
    25561 cttcttctct tgtttccgct ctatgagaaa aatcttcggt tttaagttta ctaaatttga
    25621 atgatttaga atcaactgtt ttatcttctg agtatttatg gacatttaaa ttaatatttc
    25681 catcacctct taactcatag ataaacatgg cttgtgcagt tttgcctttt ttaatttgat
    25741 cttggttatg ttctgtccaa tctttatatt ttttatcact taaaagataa ccatctctta
    25801 atttatttac tgtattttta tcatcttgag tgatattaat atagtcatga gaaatagaag
    25861 atggatttaa atctttatcg tcttttttag cagtaatttc cattttaaaa gcgatatatt
    25921 tctttttctc atctttttca ttgatgataa acggttcttt tattttagct tcaaatttgt
    25981 cactaacaat agtatcgcct ttaattttta tatccatatt ttttttgctt ttaaattctt
    26041 taagttcttc atttaattct tcattgtcat tttctttctt tttgtgacta gtgctctctt
    26101 tttttgcact atcttgatga tgtccacaag cacctaagat aagtgtactt gctaataata
    26161 tccccattac ttttttcatt taacatgtct cctttatttc gcaaaaattt attttaaaaa
    26221 ctctaaatga cttatcattt tgagtaatta aacaaagttg atattttgtg agattctaag
    26281 atgatattaa ataattcttg taataatgat cctatgtatt gttgcaataa attaatgaaa
    26341 ctataattac taatattata ttacttttat tgatagaaat atattacttt tttaaaaaaa
    26401 cttgtaatat atcgaaagat ttaaatgtaa aattttgatt tgttaagaaa ttacgtttgt
    26461 aaaaataaaa aaatcaactt atttgtatga gataaatatg tattgaagaa gacgtgttat
    26521 taatttggaa aatactggtc aaagatggga gctcttaaaa gcgttattgt attttttagt
    26581 caatacaaat agattgccgt aataataatc gtacttgatg gttaaaaaat tacttaaggc
    26641 tataaagcaa aactttttat atgagcagtc gaatataacg tttaaaatga ttgtttttgg
    26701 atataaacga ttaagtaaaa tgctttttca gtttgaaatt aatcatataa atttcttatg
    26761 ggagggttga tatcttaatg attaacatta tttcagctat aggatctatt ggaacattta
    26821 ttatggcttt attttatttt gtatcagttt cagttcaact ttatcaaatg aaaattagct
    26881 ttctgccagc tttaggtttt aaccaaattt tattagaaag ggaggaggat caacttaata
    26941 taatgaattc ggcaacagaa gagcatcatc ataaagatta tattaaacta tataatttag
    27001 gtggcggtgc tgctaaaaaa attgcaatag aggttttatt ggggaaggat aaagtcattc
    27061 agaaaaaata cgtgcatatt ttacctagta aagaagggta catgttacca attaataaaa
    27121 atgtgtacga agaattagaa agaacgattg agaacaatgg tcatgaagct gatttgaatg
    27181 tacgtatgac ttattatcat aatgtaagtc gcaaacaata ggaagttata ttaaaaggtc
    27241 aaatcgaccg ttttaatact tataataata aagaaattta tgatttgcag tttatctaaa
    27301 aattgattta agagggtagt tgtttattgc gaaaaatatc attcaatttt aatgaaataa
    27361 tggcgtcatt actataaaat attactttat gttgtaatgc atttttctat aagatagaac
    27421 taaaaggagg ggcaaagatg caaattagac aaatacatca acatgacttt gctcaagtgg
    27481 accagttaat tagaacggca tttgaaaata gtgaacatgg ttatggtaat gaatcagagc
    27541 tagtagacca aattcgtcta agtgatacgt atgacaatac cttagaatta gtagctgttc
    27601 ttcaaaatga agttgtaggg cacggtttac taagtgaagt ttatcttgat aacgaggcac
    27661 aacgggaaat tggattagtg ttagcacctg tatctgttga tattcatcat caaaataaag
    27721 gtattgggaa gcgattgatt caagcattag aacgagaagc aatattaaaa ggatataatt
    27781 ttatcagtgt attaggatgg ccgacgtatt atgccaatct aggatatcaa cgcgcaagta
    27841 tgtacgacat ttatccacca tatgatggta taccagacga agcgttttta attaaagaat
    27901 taaaagtgaa cagtttagcg ggaaaaacag gtaccataaa ttacacatct gcttttgaaa
    27961 aaatatgatt tcaagctagg attacattag gtagagttca tattaataat aaaaaatgtt
    28021 tgcaatcaaa tcgtacgttg tcgtttgtaa ttcttaaaat agcaataaat aaaatgtttg
    28081 ttagtaaagt attattgtgg ataataaaat atcgataaaa attaattgct ataatgcaat
    28141 tttagtgtat aattccatta acagagatta aatatatctt taaagggtat atagttaata
    28201 taaaatgact ttttaaaaag agggaataaa atgaatatga agaaaaaaga aaaacacgca
    28261 attcggaaaa aatcgattgg cgtggcttca gtgcttgtag gtacgttaat cggttttgga
    28321 ctactcagca gtaaagaagc agatgcaagt gaaaatagtg ttacgcaatc tgatagcgca
    28381 agtaacgaaa gcaaaagtaa tgattcaagt agcgttagtg ctgcacctaa aacagacgac
    28441 acaaacgtga gtgatactaa aacatcgtca aacactaata atggcgaaac gagtgtggcg
    28501 caaaatccag cacaacagga aacgacacaa tcatcatcaa caaatgcaac tacggaagaa
    28561 acgccggtaa ctggtgaagc tactactacg acaacgaatc aagctaatac accggcaaca
    28621 actcaatcaa gcaatacaaa tgcggaggaa ttagtgaatc aaacaagtaa tgaaacgact
    28681 tctaatgata ctaatacagt atcatctgta aattcacctc aaaattctac aaatgcggaa
    28741 aatgtttcaa caacgcaaga tacttcaact gaagcaacac cttcaaacaa tgaatcagct
    28801 ccacagagta cagatgcaag taataaagat gtagttaatc aagcggttaa tacaagtgcg
    28861 cctagaatga gagcatttag tttagcggca gtagctgcag atgcaccggc agctggcaca
    28921 gatattacga atcagttgac gaatgtgaca gttggtattg actctggtac gactgtgtat
    28981 ccgcaccaag caggttatgt caaactgaat tatggttttt cagtgcctaa ttctgctgtt
    29041 aaaggtgaca cattcaaaat aactgtacct aaagaattaa acttaaatgg tgtaacttca
    29101 actgctaaag tgccaccaat tatggctgga gatcaagtat tggcaaatgg tgtaatcgat
    29161 agtgatggta atgttattta tacatttaca gactatgtaa atactaaaga tgatgtaaaa
    29221 gcaactttga ccatgcccgc ttatattgac cctgaaaatg ttaaaaagac aggtaatgtg
    29281 acattggcta ctggcatagg tagtacaaca gcaaacaaaa cagtattagt agattatgaa
    29341 aaatatggta agttttataa cttatctatt aaaggtacaa ttgaccaaat cgataaaaca
    29401 aataatacgt atcgtcagac aatttatgtc aatccaagtg gagataacgt tattgcgccg
    29461 gttttaacag gtaatttaaa accaaatacg gatagtaatg cattaataga tcagcaaaat
    29521 acaagtatta aagtatataa agtagataat gcagctgatt tatctgaaag ttactttgtg
    29581 aatccagaaa actttgagga tgtcactaat agtgtgaata ttacattccc aaatccaaat
    29641 caatataaag tagagtttaa tacgcctgat gatcaaatta caacaccgta tatagtagtt
    29701 gttaatggtc atattgatcc gaatagcaaa ggtgatttag ctttacgttc aactttatat
    29761 gggtataact cgaatataat ttggcgctct atgtcatggg acaacgaagt agcatttaat
    29821 aacggatcag gttctggtga cggtatcgat aaaccagttg ttcctgaaca acctgatgag
    29881 cctggtgaaa ttgaaccaat tccagaggat tcagattctg acccaggttc agattctggc
    29941 agcgattcta attcagatag cggttcagat tcgggtagtg attctacatc agatagtggt
    30001 tcagattcag cgagtgattc agattcagca agtgattcag actcagcgag tgattcagat
    30061 tcagcaagcg attccgactc agcgagcgat tccgactcag acaatgactc ggattcagat
    30121 agcgattctg actcagacag tgactcagat tccgacagtg actcagattc agatagcgat
    30181 tctgactcag acagtgactc agattcagat agcgattcag attcagatag cgattcagat
    30241 tccgacagtg attccgactc agacagcgat tctgactccg acagtgattc cgactcagac
    30301 agcgattcag attccgacag tgattccgac tcagatagcg attccgactc agatagcgac
    30361 tcagattcag acagcgattc agattcagac agcgattcag attcagatag cgattcagat
    30421 tccgacagtg actcagattc cgacagtgac tcggattcag atagcgattc agattccgac
    30481 agtgactcag attccgacag tgactcagac tcagacagtg attcggattc agcgagtgat
    30541 tcggattcag atagtgattc cgactccgac agtgactcgg attcagatag cgactcagac
    30601 tcggatagcg actcggattc agatagcgat tcggactcag atagcgattc agaatcagac
    30661 agcgattcag aatcagacag cgattcagat tcagacagcg actcagacag tgactcagat
    30721 tcagatagtg actcggattc agcgagtgat tcagactcag gtagtgactc cgattcatca
    30781 agtgattccg actcagaaag tgattcaaat agcgattccg agtcaggttc taacaataat
    30841 gtagttccgc ctaattcacc taaaaatggt actaatgctt ctaataaaaa tgaggctaaa
    30901 gatagtaaag aaccattacc agatacaggt tctgaagatg aagcaaatac gtcactaatt
    30961 tggggattat tagcatcaat aggttcatta ctacttttca gaagaaaaaa agaaaataaa
    31021 gataagaaat aagtaataat gatattaaat taatcatatg attcatgaag aagccacctt
    31081 aaaaggtggc ttttttactt ggattttcca aatatattgt ttgaatataa ttaataatta
    31141 attcatcaac agttaattat tttaaaaagg tagatgttat ataatttggc ttggcgaaaa
    31201 aatagggtgt aaggtaggtt gttaattagg gaaaattaag gagaaaatac agttgaaaaa
    31261 taaattgcta gttttatcat tgggagcatt atgtgtatca caaatttggg aaagtaatcg
    31321 tgcgagtgca gtggtttctg gggagaagaa tccatatgta tctgagtcgt tgaaactgac
    31381 taataataaa aataaatcta gaacagtaga agagtataag aaaagcttgg atgatttaat
    31441 atggtccttt ccaaacttag ataatgaaag atttgataat cctgaatata aagaagctat
    31501 gaaaaaatat caacagagat ttatggctga agatgaggct ttgaagaaat tttttagtga
    31561 agagaaaaaa ataaaaaatg gaaatactga taatttagat tatctaggat tatctcatga
    31621 aagatatgaa agtgtattta atactttgaa aaaacaaagt gaggagttct taaaagaaat
    31681 tgaagatata aaaaaagata accctgaatt gaaagacttt aatgaagagg agcaattaaa
    31741 gtgcgactta gaattaaaca aattagaaaa tcagatatta atgttaggta aaacatttta
    31801 tcaaaactat agagatgatg ttgaaagttt atatagtaag ttagatttaa ttatgggata
    31861 taaagatgaa gaaagagcaa ataaaaaagc agttaacaaa aggatgttag aaaataaaaa
    31921 agaagactta gaaaccataa ttgatgaatt ttttagtgat atagataaaa caagacctaa
    31981 taatattcct gttttagaag atgaaaaaca agaagagaaa aatcataaaa atatggctca
    32041 attaaaatct gacactgaag cagcaaaaag tgatgaatca aaaagaagca agagaagtaa
    32101 aagaagttta aatactcaaa atcacaaacc tgcatctcaa gaagtttctg aacaacaaaa
    32161 agctgaatat gataaaagag cagaagaaag aaaagcgaga tttttggata atcaaaaaat
    32221 taagaaaaca cctgtagtgt cattagaata tgattttgag cataaacaac gtattgacaa
    32281 cgaaaacgac aagaaacttg tggtttctgc accaacaaag aaaccaacat caccgactac
    32341 atatactgaa acaacgacac aggtaccaat gcctacagtt gagcgtcaaa ctcagcaaca
    32401 aattatttat aatgcaccaa aacaattggc tggattaaat ggtgaaagtc atgatttcac
    32461 aacaacgcat caatcaccaa caacttcaaa tcacacgcat aataatgttg ttgaatttga
    32521 agaaacgtct gctttacctg gtagaaaatc aggatcactg gttggtataa gtcaaattga
    32581 ttcttctcat ctaactgaac gtgagaagcg tgtaattaag cgtgaacacg ttagagaagc
    32641 tcaaaagtta gttgataatt ataaagatac acatagttat aaagaccgaa taaatgcaca
    32701 acaaaaagta aatactttaa gtgaaggtca tcaaaaacgt tttaataaac aaatcaataa
    32761 agtatataat ggcaaataat taatgcatgg ctgcaaagca aataatgagt ttgtcgtaaa
    32821 aataacaaca ttttaaacta gcaataaata atatcaaagt catcatttca atgatgcaat
    32881 ctagtatagt ccacattcta aacaggtgtg gactattact tttttcactt tatattacga
    32941 aaaaattatt atgcttaact atcaatatca ataattaatt ttaagctgaa aaacaataaa
    33001 aatgttaaga caacgtttac ttcaagttaa ttattatact gaaaattctg gtatataatg
    33061 ctgttagtga atataacagg gaaattatat tggttataat attgagtcta tataaaggag
    33121 aaataacaga tgaaaaagaa attattagtt ttaactatga gcacgctatt tgctacacaa
    33181 attatgaatt caaatcacgc taaagcatca gtgacagaga gtgttgacaa aaaatttgta
    33241 gttccagaat caggaattaa taaaattatt ccagcttacg atgaatttaa gaattcgcca
    33301 aaagtaaatg ttagtaattt aactgacaat aaaaactttg tagcttctga agataaattg
    33361 aataagattg cagattcatc ggcagctagt aaaattgtag ataaaaactt tgtcgtacca
    33421 gaatcaaagt taggaaacat tgtgccagag tacaaagaaa tcaataatcg cgtgaatgta
    33481 gcaacaaaca atccagcttc acaacaagtt gataagcatt ttgttgctaa aggcccagaa
    33541 gtaaatagat ttattacgca aaacaaagta aaccaccact tcattactac gcaaacccac
    33601 tacaagaaag ttattacttc atacaaatca acacatgtac ataaacatgt aaatcatgca
    33661 aaggattcta ttaataaaca ctttattgtt aaaccatcag aatcgcctag atatacacat
    33721 ccatctcaat ctttaattat caagcatcat tttgcagttc ctggatatca cgcgcataaa
    33781 tttgttacac cagggcatgc tagcattaaa attaatcact tttgtgttgt gccacaaata
    33841 aatagtttca aggtaattcc accatatggt cacaattcac atcgtatgca tgtaccaagt
    33901 ttccaaaata acacaacagc aacacatcaa aatgctaaag taaataaagc atatgactat
    33961 aaatacttct attcttataa agtagttaaa ggtgtgaaga aatatttctc attttcacaa
    34021 tcaaatggtt ataaaattgg gaaaccatca ttaaatatca aaaatgtaaa ttatcaatat
    34081 gctgttccaa gttatagccc tacacactac gttcctgaat ttaagggtag cttaccagca
    34141 ccacgagtat aaaaattggc actaagttta cgagatatga taaataccta ttattttaaa
    34201 tatagtctac aatctatgtg gttgtaggct gtattttttg cagtttatca ataaacaccc
    34261 atcaacaaat tataccgttt ttctactttg aaagttggaa gtaacataat cttaaataaa
    34321 tatattatta attaagataa atataatact caagattatt gttaatagtt tgttcatcgc
    34381 aagttaatta ttgtttctaa aatattggta tataattttc aatggcgaag aaaacagggt
    34441 gaaaaagtcg gtttttatat caaagcaaat aagggagcat aaacaaatga aaaggaaagt
    34501 attagtacta acaatgggtg taatttgtgc aactcaatta tggcattcta atcacgcaaa
    34561 cgcattagta acagagagtg gcgcaaatga tactaagcaa tttactgaaa ttgtatcgga
    34621 agagaaagtt ataacagttg aacatgctca aattaatatt tttcaatcta atagcaattc
    34681 aaacttgatg gagttcaaca tattaacaat gggcggtaaa tcaggagcta tggttggtta
    34741 tagtgaaatt gactcatcac atttcacaga ccgtgacaaa cgcgttatta gacgtgatca
    34801 tgttaaagaa gcacaaagct tagtagagaa ctataaagat acacaaagtg ctgatgctag
    34861 gatgaaagcc aaacaaaaag ttaacacatt aagcaaaccg catcaaaact atttcaataa
    34921 acaaattgat aaggtttata atggattaca acgctaatcc aaagtaaatt ataagttata
    34981 catctcgttt ttaaatgaca atttatcccc gtaaatatta taaataatct tttcaaattc
    35041 cacatagata tagagacact aataaacctc tttgtctcga tatgatagtc tgcaacgatt
    35101 catgttgtag gctttttaat tttacaaata aggctaaata tataagttct ggcacctaaa
    35161 atatagaaaa tacataaaag taagtatagt tattttatta taattattaa atttttatta
    35221 attaattgta aaaatgtaga attataatta attaacgttt aatattaaaa ttaactaaaa
    35281 agaaagaggt gttagttatg acagaatact tattaagtgc tggcatatgt atggcaatcg
    35341 tttcaatatt acttataggg atggctatca gtaatgtttc gaaagggcaa tacgcaaaga
    35401 ggtttttcta tttcgctact agttgtttag tgttaacttt agttgtagtt tcaagtctaa
    35461 gtagctcagc aaatgcatca caaacagata atggcgtaaa tagaagtggt tctgaagatc
    35521 caacagtata tagtgcaact tcaactaaaa aattacataa agaacctgcg acattaatta
    35581 aagcgattga tggtgatacg gttaaattaa tgtacaaagg tcaaccaatg acattcagac
    35641 tattattggt tgatacacct gaaacaaagc atcctaaaaa aggtgtagag aaatatggtc
    35701 ctgaagcaag tgcatttacg aaaaaaatgg tagaaaatgc aaagaaaatt gaagtcgagt
    35761 ttgacaaagg tcaaagaact gataaatatg gacgtggctt agcgtatatt tatgctgatg
    35821 gaaaaatggt aaacgaagct ttagttcgtc aaggcttggc taaagttgct tatgtttata
    35881 aacctaacaa tacacatgaa caacttttaa gaaaaagtga agcacaagca aaaaaagaga
    35941 aattaaatat ttggagcgaa gacaacgctg attcaggtca ataatgctca ttgtaaaagt
    36001 gtcactgctg ctagtggcac ttttataatt tttagatcac gatatgattt attatcaatt
    36061 cagaattaaa aaagtaaata gtatcaaaag taagtgtatt taatattaga aaataaaaat
    36121 tttaaattta gtattaaaat ggaatgttac tatatagttc aatgtgtatt atcacagaaa
    36181 ataaaataat gctttacttc tatatttaaa agtgtataat gaaagttaag taataaagag
    36241 cgtgaagaaa aatgtgagtt atttatatag aatattctcc ttttcattta tgaatttgtt
    36301 acaaaatatt tagtgcaaaa gcacgacgga ggtattcaat atgaataacg gtacagttaa
    36361 atggtttaat gcagaaaaag gttttggttt catcgaaaga gaagatggta gcgacgtatt
    36421 cgtacacttc tcagcaatcg ctgaagatgg atacaaatca ttagaagaag gccaaaaagt
    36481 tgaattcgac atcgttgaag gcgaccgtgg cgagcaagct gcaaacgtag ttaaaatgta
    36541 attttaactt attcaaacag tccttactat agggctgttt ttttatgctt taaatcgata
    36601 acagttggtg tggtaaaagc actagccgtt atttttttgt ccaataaatt tagttggaga
    36661 tttaacaata tataatggtt ctaaaataaa tcgaactgat ggaaaagttt tttacttttc
    36721 atctgtccga cttttgattt tgaatataaa aaagcgccaa tacagaactt taataatgac
    36781 gagaattaaa gtctgtatat ggcgataaca agaagtaatg ttaaacactc aaaatgttta
    36841 acaat
//
LOCUS       DANJFZ010000016        36225 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.016, whole genome shotgun sequence.
ACCESSION   DANJFZ010000016 DANJFZ010000000
VERSION     DANJFZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.016"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(49..1122)
                     /locus_tag="PWN45_000657"
     CDS             complement(49..1122)
                     /locus_tag="PWN45_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0622443.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(1156..1257)
                     /locus_tag="PWN45_000658"
     CDS             complement(1156..1257)
                     /locus_tag="PWN45_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622444.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(1318..2604)
                     /gene="hemL"
                     /locus_tag="PWN45_000659"
     CDS             complement(1318..2604)
                     /gene="hemL"
                     /locus_tag="PWN45_000659"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL0622445.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(2652..3626)
                     /gene="hemB"
                     /locus_tag="PWN45_000660"
     CDS             complement(2652..3626)
                     /gene="hemB"
                     /locus_tag="PWN45_000660"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL0622446.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(3629..4297)
                     /locus_tag="PWN45_000661"
     CDS             complement(3629..4297)
                     /locus_tag="PWN45_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL0622447.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(4319..5245)
                     /gene="hemC"
                     /locus_tag="PWN45_000662"
     CDS             complement(4319..5245)
                     /gene="hemC"
                     /locus_tag="PWN45_000662"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL0622448.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(5287..6102)
                     /gene="ccsA"
                     /locus_tag="PWN45_000663"
     CDS             complement(5287..6102)
                     /gene="ccsA"
                     /locus_tag="PWN45_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL0622449.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(6124..7470)
                     /locus_tag="PWN45_000664"
     CDS             complement(6124..7470)
                     /locus_tag="PWN45_000664"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL0622450.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(7687..8277)
                     /locus_tag="PWN45_000665"
     CDS             complement(7687..8277)
                     /locus_tag="PWN45_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL0622451.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(8431..9693)
                     /gene="clpX"
                     /locus_tag="PWN45_000666"
     CDS             complement(8431..9693)
                     /gene="clpX"
                     /locus_tag="PWN45_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL0622452.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(9844..11145)
                     /locus_tag="PWN45_000667"
     CDS             complement(9844..11145)
                     /locus_tag="PWN45_000667"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL0622453.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(11193..11303)
                     /locus_tag="PWN45_000668"
     CDS             complement(11193..11303)
                     /locus_tag="PWN45_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622454.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(11308..12237)
                     /locus_tag="PWN45_000669"
     CDS             complement(11308..12237)
                     /locus_tag="PWN45_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622455.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(12256..12864)
                     /locus_tag="PWN45_000670"
     CDS             complement(12256..12864)
                     /locus_tag="PWN45_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL0622456.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(13006..13362)
                     /gene="rplT"
                     /locus_tag="PWN45_000671"
     CDS             complement(13006..13362)
                     /gene="rplT"
                     /locus_tag="PWN45_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL0622457.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(13409..13609)
                     /gene="rpmI"
                     /locus_tag="PWN45_000672"
     CDS             complement(13409..13609)
                     /gene="rpmI"
                     /locus_tag="PWN45_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL0622458.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(13638..14165)
                     /gene="infC"
                     /locus_tag="PWN45_000673"
     CDS             complement(13638..14165)
                     /gene="infC"
                     /locus_tag="PWN45_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL0622459.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(14194..14319)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(14394..15887)
                     /locus_tag="PWN45_000674"
     CDS             complement(14394..15887)
                     /locus_tag="PWN45_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0622460.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(16313..18250)
                     /gene="thrS"
                     /locus_tag="PWN45_000675"
     CDS             complement(16313..18250)
                     /gene="thrS"
                     /locus_tag="PWN45_000675"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL0622461.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(18448..18570)
                     /locus_tag="PWN45_000676"
     CDS             complement(18448..18570)
                     /locus_tag="PWN45_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622462.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(18663..19583)
                     /gene="dnaI"
                     /locus_tag="PWN45_000677"
     CDS             complement(18663..19583)
                     /gene="dnaI"
                     /locus_tag="PWN45_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL0622463.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(19583..20983)
                     /locus_tag="PWN45_000678"
     CDS             complement(19583..20983)
                     /locus_tag="PWN45_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDL0622464.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(20984..21454)
                     /gene="nrdR"
                     /locus_tag="PWN45_000679"
     CDS             complement(20984..21454)
                     /gene="nrdR"
                     /locus_tag="PWN45_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL0622465.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(21664..22689)
                     /gene="gap"
                     /locus_tag="PWN45_000680"
     CDS             complement(21664..22689)
                     /gene="gap"
                     /locus_tag="PWN45_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0622466.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(22859..23482)
                     /locus_tag="PWN45_000681"
     CDS             complement(22859..23482)
                     /locus_tag="PWN45_000681"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL0622467.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(23498..24370)
                     /gene="mutM"
                     /locus_tag="PWN45_000682"
     CDS             complement(23498..24370)
                     /gene="mutM"
                     /locus_tag="PWN45_000682"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL0622468.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(24386..27016)
                     /gene="polA"
                     /locus_tag="PWN45_000683"
     CDS             complement(24386..27016)
                     /gene="polA"
                     /locus_tag="PWN45_000683"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL0622469.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(27310..28797)
                     /locus_tag="PWN45_000684"
     CDS             complement(27310..28797)
                     /locus_tag="PWN45_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622470.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            complement(29296..30957)
                     /locus_tag="PWN45_000685"
     CDS             complement(29296..30957)
                     /locus_tag="PWN45_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDL0622471.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            complement(30957..31661)
                     /locus_tag="PWN45_000686"
     CDS             complement(30957..31661)
                     /locus_tag="PWN45_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0622472.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            complement(32051..33319)
                     /gene="icd"
                     /locus_tag="PWN45_000687"
     CDS             complement(32051..33319)
                     /gene="icd"
                     /locus_tag="PWN45_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL0622473.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            complement(33368..34489)
                     /locus_tag="PWN45_000688"
     CDS             complement(33368..34489)
                     /locus_tag="PWN45_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL0622474.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            34837..36198
                     /locus_tag="PWN45_000689"
     CDS             34837..36198
                     /locus_tag="PWN45_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0622475.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
BASE COUNT        11329 a         6843 c         4959 g        13094 t
ORIGIN      
        1 taatgaattc tatcctaagc ataattcaac ttagatggat gatagtttct aatcttcacg
       61 cttattcgaa cgatatttta aaaagtaatt aattcctggt gcaataataa ataggatgaa
      121 aaagattcgg aaaatatgat aactcgtaat catagcaaca tcggcaccag tagctaatgc
      181 aactaaaact atctgattaa cccctcctgg tgctgcacca agaaacaatt cattaatagg
      241 attattatca aagaaatgta tgatataaac catgattagc gcaccaatta tcaacataat
      301 attttgaatt gtaattgcga ttgctagtct accttttaaa tctgacaata aatgcgcaat
      361 ttgaactcca attctaatca tatatattag ttgtgccatg ttcaacaacc aatgatctag
      421 tgtaaatgtt aaacctgtag aaaaattcca aacaattaat acaatgagtg gtgctaataa
      481 ttgaaatgtt ggaaacttta ttttagacat aattagataa actataaaga tagctatcgc
      541 taaaataact atttgcccta tgtttaatac ttgtgataaa ggcaagactt ttgttaactt
      601 tccattcgca tgcatgttac catcatgaaa aaaatatgaa atgaacggta ctaaaacaac
      661 aacaaatata attcgtgatg tttgcgttaa gctaacaact aacaaattag cacgtttgtc
      721 ttgttcagcc atgaccagca tttgtgttag tgctcctggt ataacactta aaatagctgt
      781 ttctgtatta atacgtgcaa tttttttaaa aacaaatgcc attactattg caattaataa
      841 tatcgaaata gatacaacaa taatcgaaag ccaattgttt ttaatatcca taacgacatt
      901 tttcgtaaac gttgatccga tttgcacacc taatagtaca atacctaatt cactaagtaa
      961 gaatggccat ttaatatcaa gtttgaaaac ttttacacaa atgattgatg cgataatagg
     1021 accaaacata aatggaagta atacgtgcga tgaatacagt agaatactaa taaaaaatga
     1081 taaaacgaac acaatgaaat tatttctata tatcattgcc atgttttcca cttctttcaa
     1141 taaaaaataa aatgactaaa ttgctgcttg agcttcacgt ttgttaagat aacaatatcc
     1201 gctagcagtt ttgactacaa agcatatatg gactttcact atcaagtcgc cgcccatgcc
     1261 ttatatacat ttaaaaagag cctgaacaaa gttcaggctc tcaatttgtc cgtatattta
     1321 ttttacaata cgacttaaag ccgtatcaaa tgcttgaatc gttttttcaa tatcttcttt
     1381 cgtgtgtgcc gtagataaga atgtaccttc aaattgagat ggtggtaaaa acacaccttc
     1441 ttttgccatt tctcgataca tttctgcaaa taatttcaaa tcacttttat tcgcttgttc
     1501 aaaattagtt acaggtcctt catttaagaa ataaccaatc attgaacctg ctctatttac
     1561 agttattggt acattgtgtt tagcaaatac acgttttaaa ccgtcttcaa gtatatcgcc
     1621 taacatatta aaatactcat atgtctctgg cgttaattgg cttaacgttt cataaccact
     1681 tgtcattgca agaggatttc ctgataacgt acccgcttga taaatatttc ctaatggtgc
     1741 tatatgatcc atgatttctt ttttaccacc aaatgcacct acaggtagtc ctccaccgat
     1801 aacttttcct aagcaagtta aatctggtgt cacaccaaag taaccttgtg cacaatgata
     1861 accgactctg aaaccagtca ttacttcatc gaaaattagc aatgcgccgt attcagtcgt
     1921 aatatctctt aatccctgta aaaaaccttc aatcggcggt acgacaccca tattaccagc
     1981 aacaggttct acgattacac cagcaatatc gtttccaaat ttttcgaaag cgattttaag
     2041 tgcatctaaa tcattgtatg gaactgtaat tgtattttta gcaatacctt caggcacacc
     2101 aggagaatcc ggcaatccta atgttgccac cccagaacca gctttgatta ataacgaatc
     2161 actatgacca tgatagcaac cttcaaattt cacaatttta tttctgccag tataaccacg
     2221 tgctaatctt aaagtatcca atgtagcttc tgtaccagat gacaccatac gcactttttc
     2281 tattgaaggt actcggtcaa taacgagctg cgccaattta ttttcaagta atgttgatgc
     2341 accaaaactt gtacctttat caattgcttc atgtaaatga ctaataactt gagggtctct
     2401 atgtcctaaa ataagtggtc cccaacttag tacatagtcg atatactcgt taccatcgat
     2461 atcataaatt tttgaacctt taccgtgatc cataaaaatt gctggtgtat ctactgattt
     2521 aaatgcgcgt actggactat ttacaccacc aggcattaaa gtttcagcaa ccttcattgc
     2581 ttcttctgat ttcgtatatc tcataaagta aattcctcct acagtttatg gaaaatcata
     2641 aatataaaac cttatttatc taaatagcga caaatgtcct ttgcaaaata cgtaataatc
     2701 atatcagcac ctgcacgttt cattgaaacc atttgttcca taacgacacg ttcttcatct
     2761 atccaaccat tttgtgccgc tgctttagtc atactatatt ctccactcac attatatgca
     2821 acaactggaa cattcgtatg atttttaaca tctcgaacta tatctaaata acttagagca
     2881 ggtttaacaa tcatcatgtc gcacccttct ttaagatcac tttctaattc acgaagtgct
     2941 tccaaacggt tagcagggtc catctgatac gtttttctat ccccaaatga tggcgctgaa
     3001 tctgctgcat ctctaaaagg tccaaagaaa cttgatgcat acttgacacc ataactcatt
     3061 ataggaatat tgtaatagcc ggcttcatct aatccacgac gaatttcagc aacaaaacca
     3121 tccatcatat tacttggcgc aataatatca gcaccagctt ccacttgaga aattgctgtt
     3181 ttaacaagta gtggcaatga tttatcattg tcaacgtcat gtgtatggtc atcaatcacg
     3241 ccacaatgac catgatcagt atattcacat aaacaagtgt ctgcaacaat taataagtca
     3301 tcatacattt ttttagcaat acgtgttgcc tgttgaataa caccatcgtg aatgtatgca
     3361 ccagtaccta tatcatcttt tgagtttgga acaccgaaaa acataatggc acgtatgcct
     3421 aagtcataag cttcttttaa ttcactttca agtaaattca aactgatttg gtatacacct
     3481 ggcaatgact taatttcttt tttcacatcg tctttttcaa ctacaaaaat tggatatatt
     3541 aaatcttctt ttcttacatg attctctcta accatatctc tcattgtcgc tgatgatctc
     3601 aatcttctat gtctatcaaa tttcattttt agcccctact ttctaaaatc ttttcaatta
     3661 gtgattcgag tgtttgaatt tctgcaattg ttactggttg ttgatatgat ttaatggtcc
     3721 gtgctgtttg ttctccaata gcaaaatacg acttgaattt tggtacaaat ccttcattaa
     3781 aataataacg tactgccgac gaacttgaaa atgttaatgc atcgatttgt tgatgttcta
     3841 tcatttcttt aacatcttgt atattttgtt tgttaggcac tgaagtatat aaatctattt
     3901 taacaacttc attatcttta gataacgctg ctaataacaa tggtctcgcc aattcactcg
     3961 aaggcaaaag tattttttgg ttagtttgat taaatgattt taaaaatcct tcttgagaaa
     4021 agtcgtttgg cataaaatca actcgaatgc caagtgattc acaatattgc gctgtcttac
     4081 ttcctatcac agcaatgtta tcaacattaa ttccttttag atatttataa aagaatttca
     4141 cagcattttt agatgaaaaa ataagccagt catagcgttg atttaacaaa tgaatatcaa
     4201 aatttagtgg ctttatatca ataaagggtt tgtgaataat tgatactaaa tcactttgca
     4261 tgtcatttgt ttgtgtcata actacaactg gcttcatatt taaacgtcac tccattattt
     4321 aatgttgttc atttaagcgt tttataattt cataagcacc ttgctctttt aatttgttac
     4381 tcactgtttt gcctaactca accggatctg ttccgttcat tgtatattca aatcgttctt
     4441 taccatctgg ggtcataatt aaacctgtaa attcgatttc gttttgatct gagattgtag
     4501 catatcctgc aattggcacc tgacaactac catccatttc tgctaaaaac gttcgttcag
     4561 cagtcacaca ttttgcaacc tcatcattat gtactttgct taataatgtt aatagttctt
     4621 catcgtcact acgacattct atccctaaag caccttgtcc gattgcaggt aacaatgtat
     4681 ctctatcaag ataagatgtt acaatatcat ctgaccagcc cattcttctt aaaccagctg
     4741 cagctaaaat aatcgcatca taatcttcag tttgtaactt ttctaatcgt gtatctatat
     4801 tacctctaat ccatttaatc tctaaattag gatacttaga taatatttgt gcaccacgac
     4861 gtaatgaact agtaccaata atactgcctt ctggcaattg ggatagtggt gtatgtgttt
     4921 tagaaatata cgcatcaaaa ggtaattctc tatcagggat acaacctaat gttaaacctt
     4981 ccggaattac acttggtacg tctttaagcg agtgtattgc catatcgata tttttttcaa
     5041 aaagttcatg ttgtatttct ttaacaaata agcctttgcc tccgacttta gacaattgtt
     5101 tatctactat acgatcgcct ttcgtgacaa tttctttaat ttcaatttct agatttggct
     5161 cgacagcttt taatttatca ataaattgct ggctttgtgt taaagctaat ttacttcttc
     5221 tggagccaac gactaattta cgcatgttca attcctccta ggaacggatt gctctagatt
     5281 attttctcaa ttcacaaaat gtgttgcaaa aaataaatta atcatattta agcaaaataa
     5341 aataatgtta tagtatatta aatatcttga attcaaccat ttgttgattc taagtaaaat
     5401 ataacttcca tataatactg taataattga agagagtatt accttcgggt caatgaatat
     5461 acgttcacca actgaaatta caccccactg tgtacctaaa ataatactaa atatgagaat
     5521 tatccaccca cttaacgttg agtaaaacac aattgattca agtgtagcaa cgctaccaat
     5581 tctaaagtat ttttgatcaa aacgtttttc cttcaaatta cggtattgca tgatatacag
     5641 taatgcattg acaaaagcta aggcaaagaa gacataactt aacacagcta gaccgatatg
     5701 gactaacagt aactcgtcta caacagcaat tttctgaacc ttattagtat aatgtgtcgg
     5761 ttgaaatgta ttcatcccta aaagtgttaa ccctattaaa ttcaagaaaa acacagagaa
     5821 attcaaaact ttgataaggt taagaattaa agagattgaa ataatcagcc aacttaaagt
     5881 atagaataca tctgaaatag accctaatgg aatatgtcta gtttgtataa taaaaataga
     5941 taaagagatt gtttgtaaaa cccaaacaat ccccaataaa tatatgccta aacttctaat
     6001 cttatgactt ttttgtacaa aatcataaaa atagcaaatg atactgatta agtatattaa
     6061 taatataatt tcattgaatc gaataaacag gttttcttgc atattatcac catattaata
     6121 tgcttattca aaactaaaga tacgtcgcgc tgaaatttcc ttgaccttgc tttctttttg
     6181 ttgcttcgct tgctcatgag gacattcagc ttcgatatca aatatatttt gaaatagctc
     6241 taatttttca ttacttttct tatcactact taattctttg gcctgtttaa taggatcttt
     6301 caacatttga ttgatgatac tttttgtatg tttagatata attttacgtt ctctttcgct
     6361 taaccctggc aatttacgat caatactgtc catcgtttct gcttgaattg ccatagcttt
     6421 ttcacgtaaa gctctaatca ctggaacaac acccaacata ctaatccact cattgtgtgc
     6481 atgtatttct gcaggaattt gttccgaaat tgttgcagcc gctaattgtc gctcacgtaa
     6541 gtttgcatca actaaacctt ttaagtcatc aacatcataa ttaaagatgt ttgtgatggc
     6601 actaatacca ggttcaatat ctcgaggaac tgcaatatca atcaatacta gtgaatcttg
     6661 ctttctattt tctgcaattc tttctatcat ttcatttgta atgatataag attgtgcact
     6721 cgttgaacta atcacaatat ctgcactttc aagtaaattt ggtaatgatg atagttcatc
     6781 atatttcact tgatgctttg ctgctaattt catagcattt tcaattgttc tatttactac
     6841 tgtaatatca gtaattccag aaccaagaag atttaatagt gataattcac tcatttcccc
     6901 tgcaccaata atgatagctt gcttactttt caatttgcca aatacttttt tcgccaactc
     6961 gaccgcagca taagacacac ttacagcatt atcagctata tctgtttcat tatgtgctct
     7021 ttttgcaaaa gtaattgcct gtttaaatag atgattaaaa attgttcctg tcgtacctgt
     7081 gctttgcgct aagaaaaatg catctcttat ttgacctaaa atttgagttt ctccaagtac
     7141 gattgaatct aaaccagaag tgacacgcaa taaatgttct actgcttcgt cccccacttt
     7201 tacttctgac attgctttaa tatcatctac ttcaaatcca aatgcacgag ctagaaatcg
     7261 ttgaatatag taacgacctg tgtgaatttg atcaacaaca gcatatactt cagttcgatt
     7321 acatgttgat aatatgacat tttctaaaat agatttagtt tcatataaat cttcatgggc
     7381 aattcgtaag gcatcatctc taaaagcaac ttgctctctt agtgcaacat cagctgtgcg
     7441 atgatttata ctaattgcaa taaaatgcat ttgaaacgcc cccatatata ctaacaatac
     7501 aaatattata acataattta aaatccaatt aagtacgatg cttaaataaa ttataatctt
     7561 tctaatttac ttttgagttt ttgacaaaat gatgaacgtt gaaaaatgtt atcaattgtg
     7621 tactaattaa aagtttcgtg cttacattat ttttaaaatt tttgtataag ttttgacgta
     7681 caaaaactat gaaatatacg gttcaattaa attccatatt tgttgttgtt tattattttg
     7741 aattgatgaa taacttacaa ttgtatcgtc tgggtccata tctaattgtg tcttaatatt
     7801 tttaatatgc ttttgaacct taccttttgg aattttgtct tctttagtgc atataactaa
     7861 agtaggaata tcaaaatgtt tcaaataatt gtacattaag atatcatctt gtgttggatc
     7921 atgtcttaaa tcaactaatt gaataactaa ttgcaaattc tctctcttag ttatatattc
     7981 ctcaatcatt ttcccaaatt tttcacgttg tgttttactt actttagcat atccataccc
     8041 tggaacatcc acaaaaataa gttgttcatc tatattataa aaatttaacg tttgcgtttt
     8101 gccgggttgc tgtgatgtac gtgccatatt ttttctgcca atcatactat tgataaatgt
     8161 agacttacct acattagatc gaccgctcag tgcaacttca gacaattctg tttctggata
     8221 ttgttcttct tttactgcac taatgattaa ttctatatta ttaggattaa ctttcatttt
     8281 atatcctcac ttttttatat tctctttcaa ttctaccatt atataatatt tgaacctttc
     8341 ggtatacatt ataaaaaagc tccgatcaaa gttaaactat ttgagcgtca actttgattg
     8401 gagcttttca cttttataac acatcaatga ttaagctgat gttttactat tattaattaa
     8461 attgccttct gcgtcgtata gttctggttc agtttcttca ttaattgttt gtgctgtaat
     8521 aactaccttc gttacatttt cgttagaagg cacatcaaac ataatatcga ttaacgattc
     8581 ttctatgatt gaacgtaaac cacgcgcacc tgtttttctt tcaattgctt tttcactaat
     8641 tgctgataaa gcttcttcag tgaactctaa atccacatca tctaattcca gcattttagt
     8701 atattgtttc acaagtgcat ttttaggttg cgttaagatg tttttcaacg cagttacatc
     8761 taatgtttct aaattagcta caattggcac acgtccgata aattcaggaa tcaaaccata
     8821 ggcttgcaaa tcttctgggc gaatttgtgc taataatgct tgttcgtcat atttatcagc
     8881 ttcattgctt gagaaaccaa taactttttc accaagacgg cgcttaatca cttcttcaat
     8941 accatcaaag gcaccaccaa gaataaataa gatatttgtt gtatcaattt gaatcatttc
     9001 ttggtttgga tgtttgcgtc caccttgtgg cggaacactt gcagtcgtac cttctaagat
     9061 tttaagcaat gcttgttgaa caccttcacc tgaaacgtca cgtgttatag atgtgttttc
     9121 agatttacgt gcaattttat caatttcatc tacataaata ataccttttt cggctttatc
     9181 aatgtcaaag tcagctgctt gaattaatct caacaagata ttttcaacat catcgcctac
     9241 ataaccagct tcagttaaac ttgtcgcatc tgcaattgca aatggtacat tcaacgtctt
     9301 ggctaaggtt tgagctaata atgttttacc actacctgtt ggcccaatta atgcaatgtt
     9361 acttttttgt aattcaacat catcttcttt tggtcctaat tgttgaatac gcttatagtg
     9421 gttataaaca gctacagcta aagatttttt agctttttct tgaccaataa catattcgtt
     9481 taaatgatcc ataatttctt taggagtagg taattctgtc atcgcttcag aagtgttttg
     9541 agctaattct tcttcgacga tttctgagca taattcaata cactcattac aaatatatac
     9601 accacttcct gctacaagtt tttttacttg atcttggtct tttccgcaga aagagcattt
     9661 caaattttct tcatcttcat tgaatttaaa cattcttttt acacccctat tcgttaaaga
     9721 ctatactaga ttggatgtta caatgcaaca tattaacata caaacttttt gcttaaagaa
     9781 tagtagcaga tacataagct atgcatctgc tactcatttt tgtaatttaa tatttaatga
     9841 agattaatct tctttagttc cttcaacgaa ctttgcgtta tctcttaata aatcgataac
     9901 tttttggata cgaacatcat ttttaatgat atcagtatta cctaaagtat ttttgatatc
     9961 ttcaactgag atattaaatt gtttactcat tttttctaat tctttatcga tatcttcatc
    10021 agtagcttcg attttttcag cttcagcgat cgcagttaaa gttaagttag ttttaacacg
    10081 ttgttctgca tcgtctttca tttgctctct taattgagtt tcatcttgac ctgagatttg
    10141 gaagtacgtt tgtaaatcta aaccttgttg ttgaattctt tgtgcaaatt cagacaccat
    10201 acgatctaat tcagtattaa ccattgcttc aggaatatcg attgttgtat tatcagtagc
    10261 ttttgtaatc gcttcttctt tttcaacatt ttcagcatct gtagcttttt gttcagctaa
    10321 acgtttacgt aagttttctt tgtactcgtc tactgtattt gcttctgcat ctaattcatt
    10381 agcaatttca tctgttaatt ctgggacttc tttaaattta atttcgttaa cttttgtttt
    10441 gaaagttgct tctttaccgg ctaattcttc agcatggtat tcttctggga atgttacgac
    10501 aacatctttt tcttcgtcaa ctttcatacc ttctaattgc tcttcgaaac caggtatgaa
    10561 tgaacctgaa ccgatttcta aatcgtaacc ttcagcttgt ccaccttcga attcttctcc
    10621 gtcaactgaa ccactaaagt cgatgttaac tgtgtcgcca ttttcaacaa caccatcttc
    10681 tttaacgacc atttcagcta aatgtcctaa gctgtggtca atcgcttctt gtaactcatc
    10741 atcagataat tcagtttctt gtttttcaat ttcaagacct ttatagtctc ctaatttaac
    10801 ttctggctca actgtaactg ttgcttcaaa aatgaaatct ttaccttttt caatttgagt
    10861 aacacttact tctggttgtg caactggttt aatatcagtt tcgtcaattg cttcaccata
    10921 agcatctggt aataaaatgt cgatagcatc ttgatataat gcttctacac caaagcgttg
    10981 ttcaaaaatt ggacgtggca ctttaccttt acggaatcca ggtacgttaa tttgtttaac
    11041 cacttttttg aatgcttgat ctaacgcttt gtttactttt tctgcaggaa cagtaacagt
    11101 taataaacct tcgttacctt cctttttttc ccaagttgct gtcatgtata tatacctcca
    11161 tgattaacta atttattttt tcaacttccc tattatatca taggtctatt ccctatacaa
    11221 acattgaaat cacaacgttt atatatttgt aaatcaactt ttttcgtcaa aactatatta
    11281 aaatgctgag tattattttg caacaattta actattatta ttcaaatcta attgctgaat
    11341 aaaattaaat gcatcgatac tttgtaattc acatttaagt cctaacatat ttttgaaata
    11401 acattcgtat gcattaatcc atttattcgt ttcaaacaaa gtttcaatat caattggata
    11461 aatcatgata gagtgattat tcataatatg gtgtgcttct tcagcgatat gtaaagcacc
    11521 atcatttaat gtttccataa cgttaggtat aactttttct ttaagtgttg tatgttctag
    11581 cccttttaaa ttagctggta caaaagttac atccatacca tacttttcaa ttgtcagttc
    11641 ttgtgtacaa tttgcgaacc ttaaatactc aatcattaaa ctaatgaggt tataactgta
    11701 cgtattagat tttaatatat ataataccgt ttcttgaaat tgaaaatgac cattgtctat
    11761 taacttcaaa atcaagtgcg tctgttccct cattgataac gtatcaaaat cagctaatga
    11821 ctgagtcaat cgtttttcat cttcaattaa ttttgactta gcaaattctt taagaggatg
    11881 taatgccatt cttgttttgt gatctttgac ttcatcaata atttgatgaa ttacttctac
    11941 cgcttcaaaa tattgtccca aaccaatcaa acttttcacg taataaatca tcaatgcatc
    12001 atattgttgt atgccagttt tcaataagac aattgtttct tctcttaatt caagaaatga
    12061 ccccgtctca tataacatac ggcatttcat cattgctatt tcttcagtga gttcaaattg
    12121 acgttcatat tgcacaatat attcatacat caggtcatag ttttggtctt gttcagcacg
    12181 ttttatatct ttatataact ttttcgaaga gttaggaaac tgaataatat ctgacatata
    12241 tgtcacctcg tttgattata aatcttgaag gattaatgca ctccacgttt cgaaatcatc
    12301 atatgactct atgttgcctt tttcaaccca ttttattcgt aaagtatctg tttctttagc
    12361 ttctacatca ttcgtactta cagtgatttt aaatacaaca ccaatatgta ccttgcccac
    12421 ttcattatta tcgtcattaa taaaaccgat atattccata ttttgtgaat cttgctcact
    12481 taaacctact tcttcttcta attctctctg tgcattaact ctcaatactt cgttaataga
    12541 ttctgctcct ggaacatcat tcatatgacc gcctacacct attgaagatt gtccatgcaa
    12601 tcgagcttct ccaccgccag ataatcgttc atacactaat atctcgccat gctcattttc
    12661 aagtaaacaa taagaaatga gttgtttata tgatggatcc tcttccatat cgccgcgtcg
    12721 cttaacttca tattgactta gcgcatcaaa aatattttga ccttctggtt tattcttatt
    12781 taaaaaccca ttgaaagtat tcttttcatt attaaaaata atttctctag gtactacaat
    12841 gatttgttca tcaaatttag acatcgtata aaactcctca ctttaatgtt tgttctctta
    12901 aatgcatttt agcaaatatg tcatatttta gcgattaaat gtggcatttt gctatttaaa
    12961 aaataaaaaa gagatgacaa tgccatctct tttttataag tgttattatt ttaaagcatc
    13021 tttagcttta gttactaatt gagcaaatgc tttttcgtca gaaattgcga tttctgataa
    13081 cattttacgg ttaatgtcga taccagcttt tttcaaaccg ttcattaaac gtgagtagct
    13141 catttcatgt tgacgagctg ctgcgttgat acgtgtaatc cataatttac ggaagtcacg
    13201 tttacgttga cgacggtcac ggaaagcata ttgacctgat ttcattactt gttgcttagc
    13261 tactttgtat aatgtatgtt ttgaaccgaa gtaaccttta gctaatttaa tcgttttttt
    13321 acgacgcgct cttgttactg ttccaccttt aactcgtggc ataaaaaatt cctccttagg
    13381 taattcctac cgatttctgt atttgttctt attttttgta tgctaataat tgttttacac
    13441 gtttcatatc gctcttagac actaatctag ctttacgtaa ttgacgtttt tgtttagtgc
    13501 tcttgtttgc gaataagtga gatgtgaaag ctcttgaacg ttttaattga cctgaagcag
    13561 ttcttttaac acgtttagct gctccgcggt gagttttcat ttttggcata atataagtcc
    13621 tcctgtaata gctttaatta tttttcagct gttggcgcta acatgataaa catttgacgc
    13681 ccgtccattt taggtttttg ttcaactgtt gctatatctt tgcattcatc tgcatatttt
    13741 tctagcacac gttgaccaat ttccttatgc gtaatggcac gccctctgaa acgaatagat
    13801 actttacatt tatcgccttt agttaagaat ttacgtccgt ttttcaactt cgtttggaaa
    13861 tcatgttcct caattgttgg acttaaacga atttctttaa cattgataat tttttgtttc
    13921 tttttcattt ctttttcttt tttctgttgt tcgaatttga atttaccgta atccataatt
    13981 cttgcaactg gtggtttcgc attcggtgca acgaccacta agtctaaatc tacacgttca
    14041 gccatttcta aagcttcacg ctttgattta acaccaattt gttcaccatc ttgaccgatt
    14101 aaacgtaatt cttttgcacg aattttgtca ttgatttgag tttgatcttt tgctatggtt
    14161 gacacctcca aaatttttac gaaatttgca ccaagcaaaa aggaagagca ggtataaaat
    14221 acccgctctt ccttatacac agttatgtgt aatgtgatta acctgccaac tgctttatgc
    14281 gtcgctacag gtgagaagcg ggtgcttcta cttggttcgt ttcgtattca acgttattaa
    14341 tcatatcaac aattcacatt taagtcaaca ctataactgt aattattttt attttaacct
    14401 tttatttcat ccattgacac gtcttgacgt aaatctactt gttctaatgg aatttttttc
    14461 gttttatatc gaagcttatg ataaataaag aatgctaaaa atactggaat tcccatatac
    14521 gtaattaaga agcgactaaa attaaaatct cctgttttaa taaagtcaac atcttgacca
    14581 ataattacta caatacataa aaagccagca aataatggtc cgaatgggaa taatttagca
    14641 gtatatttta atttagattt gtcataattt tgtttatcaa atgctcttct aaatcgataa
    14701 tgacttactg ctatacctac ccaagcaata aaaccagtta aaccacttgc tgcaacgata
    14761 tattcgtatg caccttttgt aaggctttgt aatacgaaaa ttatcactac aatgattgct
    14821 gtaacgagta acgacatata tggcacaccg tttttatttg ttttaccaaa tgcttcaaac
    14881 gctaatttat ctttactcat tgaatatagc attcgagttg aagcatacat acctgagtta
    14941 cctgctgata acacagacgt taaaatgact gcattcataa atgatgctgc aaacgcaaat
    15001 ccagcatttt taaacactaa tgtgaatgga gacgttgcta cattatcact accccccatt
    15061 aatgcactac tatcataagg aattaacata ccgataacaa aaatggctaa aatgtaaaat
    15121 aataaaattc tccagaatac ttgtttaatt gctttcggca cagcacgttc aggattttct
    15181 gattcaccag ccgtaatacc aattaactca gtaccttgga atgagaaacc agcgattaag
    15241 aatacaccta gaattgataa caaacttcct cctaagttgc caccaagaat tggaccttca
    15301 cctttattaa atatttcgaa tcctacaaca tgaccaccca tgattccgac aatcgttaat
    15361 aaaccaattg caatgaaaac aataactgta accactttta tcaatgccaa ccagtattca
    15421 ctttcaccat agacgcgaac tgataacgaa ttcagactaa aaattataac taagaacaac
    15481 gcactccatg cccaagcggg tatgccttgc aatggtgtcc aatattgaat gacttgtgct
    15541 gcaatcgtaa tatctgctgc tacagtcact acccagttaa accaatagtt ccaaccaagc
    15601 gcaaacccta aagatggatc aacaaatctt gtagcatatg tactaaatga acctgatact
    15661 ggcaaatacg tagccatttc gccaagtgac gtcattaaga aaaataccat tattccgata
    15721 attgcgtatc ctattaatgc acccaaagca cctgcatcat gaattgctcc accagaagtt
    15781 acaaataaac ctgtaccaat acaaccccca atcgcaatca tagaaatatg acgatcttta
    15841 agtccccttt tgacaacatt gttactttca ttttgaactt ttgacatata agctcccttt
    15901 ccatgtttta gaggccaaga aataaataac ctttattcac tttcataccc ttttttacag
    15961 aacatagtga tattaaaagt aaaaaagtgg ctacatacct ttaagatatg caaccacttt
    16021 ggcaattatc tatataaggt agcacatcac attctgtgac agtacagttc ctattcgaaa
    16081 actgtcccaa tagatataat ttatggttta tatctatttc ggcatcttta cctttcactt
    16141 gttcaactta tgtaccataa atacttctga caagttacta attaaacatg caacctctaa
    16201 ctcaatttaa tattttaact aacttgtaat atacaggatt catcacgcat aatcaaccct
    16261 gtaaaacttg atacgcaata aaagttttaa agcattttat tgcgacaact gtctatctat
    16321 gttttttcaa acgaatttca tcaactagat tccagataaa ttcatctttt tcaactgttt
    16381 cttggtcttg cgatccatat tgacgcacat tcacttgatt attttcaact tccttatccc
    16441 caactacgat ttgataaggt attttttgca tttgagcttc tctgatttta taacccattt
    16501 tttcattacg gtcatcaata cttacacgaa cgccttgaga tttcaattca tcttgtaatt
    16561 ggcgcgcata atcataatgt aaatcaacgt taactggaat gatttgaact tgttttggcg
    16621 ctaaccaagt tgggaatgca cctttagttt cttcagttaa aaatgctaca aatcgttcca
    16681 ttgttgatac aacaccacga tgaataacaa ctggacgatg atgttcacca tcttgaccaa
    16741 tataagttaa atcaaaacgt tctggtaata agaaatcaag ttgtgctgtt gataatgtct
    16801 cttctttacc catcgctgtt ttaacttgaa catctagttt cggaccatag aatgccgctt
    16861 caccaatcgc ttcttcgtac gataagccaa gctcatccgc tgcctcttta agcatatttt
    16921 cagctttatt ccacatatca tcatcatcaa agtacttttc tttatcttca gggtctctat
    16981 aacttaatct aaagctataa tcctcgaaac caaagtcttt atacacatca ataatcatgt
    17041 ttacaacgcg tttgaattct tctttaattt gatcaggtcg aacaaagata tgtgaatcat
    17101 ttaaagtcat accacgaaca cgttgtaatc ctgatacagc accacttgct tcatatctat
    17161 gcatcgttcc tagctcagcg atacggatag gtaattcacg atatgaatgt ggtttattcg
    17221 cataaatcat catatgatgt ggacagttca ttggacgtaa taccatagat tcagtttcat
    17281 ctaactgcat tggtgggaac atatcttctt gatagtgatc ccagtgacca gatgttttgt
    17341 ataaatcaac attagcaagt actggtgtat aaacgtggtc atatcccatg ctaacttctt
    17401 tatcaacaat gtaacgttca atttcacgtc taattgttgc accgttaggt aaccataatg
    17461 gcaaaccagc accaactaat tggctatttg tgaatagttc taactcttta ccaattttac
    17521 gatgatcacg ttctttacgc tcttctaaca tttgtaaatg tgctttcaat tcttttttat
    17581 caaagaaagc agtaccgtat atacgttgta acattttgtt gttactatct ccacgccagt
    17641 atgcacctgc tgtagataat agtttaaact ctttaatttt agctgttgat ggaacgtgaa
    17701 ctccacgaca taaatcagta aaatcacctt gactatataa tgttacattt tcatcttcag
    17761 gaatcgcgtc gattaattct aatttgtatt catcattgct gaataactct ttcgcttcat
    17821 ctcgtgaaac cacttttcgt tcgattttca tattttcgtt aacgatttgt ttcattgttt
    17881 tttcaatttg ttcaaagtca tcagatgaga tgttttggtc aatgtcgaag tcatagtaga
    17941 atccaccttc tattacagga cctacaccaa atttaacatt accatataac cttttaatcg
    18001 cgtgtgccat taaatgtgca gtagaatgac gtaatacctc taacgcttct tcactacctg
    18061 gtgtcacaat ttcaattgat ccatcagttt caagcggttt agttaaatct acaagttgcc
    18121 cgttaaattt gccggcaaca gcttttttac gtaatccagg actaattgat tgtgctatat
    18181 cttcagtagt agtaccttta tcaaacgcct ttttattacc atctggaaat tgaatattaa
    18241 tttgttccat gttaaaccct cctatttgct ttataaatac ttttatctga cctcgttaaa
    18301 aaattgaaca acaatcatcg ttttaacatt aaacctatta acaataaaca gggaatttat
    18361 gacaccaaca aacatttatg tctgaaaaaa acaaaaaatc ccgcccctat acaagggacg
    18421 agatcgtcgt ggtaccaccc tagttatcta atacaattta tgtattaaat atctctgctt
    18481 aagataacgg tcttgatccg ggtattcatt acaaatacca taaatgaagt agtaatcatc
    18541 taatttatta accatattct catcaaacaa tggctttctg tgtaatattg attagatcat
    18601 cttgtcttca atcatttaaa cgatttagat ttgtattcat tatacaccaa tcattttaaa
    18661 attcaattgt ttctgaaatt ttctcctgat aaaaagtatg gtgttgacaa agatttgaca
    18721 cgttcaataa tacgtgctgc tttagtcttc tcttcaccat cacgagtcat cgctaaatga
    18781 tgttccaatt cactatagtc aaaattagaa ctaaagaatg ttggtaattc atgaaccatt
    18841 cgataatgta gcaaaggtcc aattacctca tctctcaccc atggagtcac ttcttcagcc
    18901 ccaatatcat caagcattaa aatgtttgct tctcttacgc gatgtaattt cttttcaaaa
    18961 gaaccatctt taaagccacc ttttaatgtt ctaataaatt ccggtaaata aataattgtc
    19021 gaacgtacct tcttagattt gagctgattc gcaattgcac ctagaataaa agatttacct
    19081 gtcccaaatg gaccataaag gtaaaggcct ttcacttgtt ccccattagt tattgctgta
    19141 caaatatcat ctgctgccat agctacatca agacggtctc gatgattcat ataaatatct
    19201 ttcaatttgg catttaaagt atctcgttgc atatgatgag atgtaattag ctcagcttca
    19261 aagcgttctt cgtcgtactt gattttacat gggcattgta aatagcgtat tttaattcgg
    19321 ttattatcaa catataactc aggcacatgc ccctttacga aatttggaca atcagcaaat
    19381 ttatgaccgt cataatgttt ttgttgatct ttatactctt gtaacacatt taagtcttca
    19441 tcaatcatag cattcgttaa ttcagctcga tgcgcttcca aaaattgctt aacatctggg
    19501 tcattgatta cttctttttt tatcttttct attctttttt caaagtcctg cgacgtgtta
    19561 attatacttt taaattgctt cattattgac tgtcctcctc ccatttttta gataatttat
    19621 ctagaaatgc ttgtcgatct tgctctaatt gttgatcatc tacgctatta tctttagccg
    19681 aatcttcttc actaggttta tctctatttt ctaaccattt aggtgttttt tcttttgaaa
    19741 tacgattacg ctgcccatag tatgaaccac gcttttggta atttccgcta gaaccctcat
    19801 ttttaggttg attaactttt ttagcgtaat tatatgcttc tttagctgtc ttaatacctt
    19861 ttttcttcca atttgatgct atttccaaaa tatacgcttt aggaagtttc atatcttctt
    19921 ttaacatgac aaattgcaac aaaatattaa tgacgccaaa agacattttt tcacgttcaa
    19981 ttaattcttc aaccattgtc ttttgcgata tagttggttc tgattcagac caagaagcta
    20041 acatatcaat tggactcgtt tgttcaagta actcaaacca ttcatcactt tgtggctttg
    20101 gattcacttc tgaagatttg cccgccgaag atgatgtagc aggagatttc acctgtaatt
    20161 taggcatttg attttcgtgt tccattaagt aatacgagcg tgcttgttta cgcatttctt
    20221 caaaggataa ctgttgtcca cttgtaattg aatttaaaat aacatgcttc atgccatctg
    20281 ctgttaaacc atataaagtc gcgagttgtg taattaaacg ctttgcatct ttggtaacaa
    20341 tgtcttgact aataaaatgt ttacctaaca tttgtctcaa catttcaaag tcaaaagatt
    20401 catttgataa atcgatacct tggtacggtt cattaatcgg aatatcactt gtatcgatat
    20461 ctatttttgt agacggcact ttaaaaacat cagtaaattg tcttgttacc tgtttaaatt
    20521 cactcaaatc aatttgttga tactcaaagt atttcttcaa ctcatgaaat cgacgatgct
    20581 cgacttcact atataaaaag attgacaaca ttggatcatt aaaaaataaa tgtgctgaag
    20641 gcggttgaat taattggtaa acaaattgtg tttcttgttc atcatgtttg acaaacgcct
    20701 ttaacaatcc aatcgcttca agtaagtcca tttgttgtct aaactctagt aaattaattt
    20761 taagttcatt cataaaaata taatgagaaa gaatcaatgt ttcattatga ctttctttaa
    20821 cgaattgagt cataaaatga tataaaccca ctgcttgcgt tccaattagc ggtgtataca
    20881 gtcgattcaa tacctctaaa tgattcgtat ttaaatcaaa gtgttgcata actttgaatt
    20941 gatcctttgg tcttaagccg aattcgaagg cttgtcgtcc catttaagca tcactccgtt
    21001 tgttttcgct taaaatccct tgcatcgatg ctaacaattg atcaacatct ttaaattctt
    21061 tatagactga tgcaaatcta acatatgaaa cttgatcaac atgcattaac aagttcataa
    21121 cgtgttcacc tatatctcgt gaagacactt ccgtatgacc ttcatctcgt aattgccatt
    21181 caaccttgtt agttatgtct tcaagttgtt gatatctaac tggtcgtttc tcacaagaac
    21241 gcacaagtcc attaagtatc ttttctcttg aaaactgctc tcttgtgcca tcttttttca
    21301 caactataag ctgactaact tcgatatgtt caaatgtagt gaaacgtgtt ccacaatttt
    21361 cacattctct tcgtcttcga atggcattta attcatcggc atgccttgaa tctacaactt
    21421 tagattgtgt agaattacat ttcgggcatt tcattacatc accctcttta ttttgattat
    21481 gcctaattat actataaatc tagagatgaa aaaagaatcc ctcaatttaa ttcatttaac
    21541 caaataatga aacaataaaa aacattatat cgttacttat taagtaattt gcatgacaat
    21601 attattgtat taaaaataaa aaacctaact ccgaagtcag agttaggcta taaattaatt
    21661 gtattaactt gcacttacag tttcttttga tgtcaaaagt gctccaattt gctcagcaac
    21721 atctacaact ctatttgaat aaccccattc attatcatac caagcaataa cttttacttt
    21781 attccctgac atgaccattg ttgattttgc atcaataata gctgaatttg gattagtatt
    21841 aaaatcaaca gacactagtg gttgatgttc gacttctatg ataccttcta aacctgcatt
    21901 ttcaaaagct tggtttactt cttctgcagt tacttctttt tctaaatcaa caactaaatc
    21961 aacgagcgat acattctttg ttggtacacg taatgccatg ccgtgtaatt taccttctaa
    22021 ttctggtaat acttctttta aagctttcgc cgcaccagta gaagtaggaa taatgctttc
    22081 attacatgaa cgtgcacgtc ttaaatcttt atgtggatta tcaatatttt tttggtcatt
    22141 tgtaatagcg tgaacagtag tcattaaacc attaactatt ccaaactgat tatttaaaac
    22201 ttttgcaact ggaccaatgc aattagtagt acatgaagca ttactaaaaa tgtcaaatgc
    22261 ttctatatct aattggttat catttacgcc tttaactacc atttgaacat gtccaccttt
    22321 tgaaggacca gttaacaaaa cttttttggc acctgcttta atatgtgcga tggctttatc
    22381 accatgatta aatttaccag ttgcatctat agcaatatcg atatctaatt ctttccatgg
    22441 caagttttca ggattgcgat cagcaaccaa tttaatttta tgatctccaa cttgcaatcc
    22501 attttcaatc ggttcaactt ttagattata ttttccatgt gtcgtatcgt aattgattaa
    22561 atgtgcaatt gtttcgggtg gataactagc atttatcgct actacattta aatttttatt
    22621 ttgtaatgca atacgtaata ccattcttcc aattctaccc ataccattaa ttgcaatatt
    22681 cgttgacatt aaaagcaccc cttgatcttt atgtgttata ctttatggaa ttagtatagc
    22741 atattatatg aaatttgaaa gcgttttccg ttgatttatt tatctttttt ataaaaaata
    22801 gtcattactt acatacatca acgcgtacgt aaagtaatga cttatttata gtgtaatatt
    22861 aatcttcttc tccgtaattc ggcttttcaa tataaccttc ttcttctaac aatctctcaa
    22921 ggttttgttt taattcaagt ttatccccta aattatcgat aacatgatcg gccattcggc
    22981 tttttttatc aatagaaatt tggctataga cacgtgcttt cgcatcttct aatgacaaat
    23041 tattacgttg cattaaacga tccatttgta tactttcaga agtgtataca acccacactt
    23101 cgtctactgt attttccaat tcattttcaa ataataatgg aatatccatg attacattat
    23161 atccttgttt taaatattct tgcttttctt cttccataat atctcgcacg ataggatgta
    23221 tgatagcatt taattctaag cgtttttctg gatgattaaa cactagatca cccatataac
    23281 gacgattcat ctcaccattt tcatcaattg cttcatcacc aaagacttct cgtacttgag
    23341 ctaaaccttt actccccttt ttaacagctt ccctggctgc tttatcagca tctactactt
    23401 taaaaccgaa tacggataag agttctgata ctgttgattt tcctgaggcg attccacctg
    23461 ttagaccaat aactttcggc ataatttcac tctttcttta tttttgacat actggacaat
    23521 aatgactatt tcttgtcgcg atgatttttg tttcaatttg acttccacac actttgcata
    23581 ccggctgctt atatacatta agatgcaatt gcatctcacc agtttttcca tcagcatgac
    23641 gataatctga aatacttgta ccgccatatt taataccttc ttctagtact tctctaacat
    23701 aataaaaaac catttcttgt tgttggtgtg ttaagtcttt tactttttta tctggtaaaa
    23761 cacctgcacg aaacaacgct tcacatgcgt aaatatttcc acaacctgcg attactttat
    23821 gatccaaaat cacttgtttg attggtttat tcttattaga ctgttgatga attcgattta
    23881 aataatacgt caatgcttca tttgaaaaag gttcaggcgc tatttctaaa aatgaaggat
    23941 aagatgctac agacgcaaca tttctaattt ctccaaaacg acgtatatct gaataaatta
    24001 actttttgtc atttgacaac tcaaaaataa catgccaatg cttacgataa ttaggtatca
    24061 taatatcttc aagttcatct acaatgaaaa aaccgcccgc catacctaaa tgactaatta
    24121 atgtacgttg ttctcgttta ttatctagct gaaaaacgat atatttactt cttcgttcta
    24181 catttgtaat ggtatagcct tccgataaag ttttaaaagt atctaattca attcctttta
    24241 taattgtttc cttgccttga gctttacctt cgattacttt atccgaaaat ataacgtgtt
    24301 caattttttg atttataacg tagggttcaa ttcctctttt tacatgttct acttctggta
    24361 attcgggcat accattaacc tcactttatt ttgcatcata ccaggttgca ccataacttg
    24421 agtctacttt taatggaaca tctaattgca atgcattttc cattatctct tctacaaatt
    24481 cactaaatga atctacttct gacttaggta cttcaaaaat taattcatcg tgtacttgta
    24541 ataatagttt agcttgatat gttgtctctt tcattttttg agcaaattta accattgcca
    24601 gtttaatgat atctgcagca ctgccctgta ttggcgtatt catagcagta cgttcagcaa
    24661 agccgcgtaa attaaagtta cgactcgtaa tatcaggaat atagcgtcga cgatgtagca
    24721 atgtttccac gtaacctaaa gctttggcat cttttacaat atcagacata tattgtttta
    24781 cacctgggaa actagctaaa taatcatcaa tgaatgcttt tgctttttta cgagtaatac
    24841 ctaaactttg acttaaacca taatcactta tcccataaac aattccaaag ttaactgctt
    24901 ttgcttgacg acgcattaaa ctatcgactt gatcagcttc tacaccaaat actttcatag
    24961 cagttgctgt atgaatatca tcgccgttga taaatgcttc tttcatactc tcatcttgtg
    25021 taatgtgtgc taatacacgc aattcaattt gagaataatc tgctgataat ataacgctat
    25081 cttttgaagt tggtttaaag gcttttctta ttttacgccc ttcttcaagt ctaaccggaa
    25141 tattttgtaa attaggatct acacttgata aacgtccagt ttgcgctaaa gtttgattaa
    25201 aacgtgtatg gatacgttga tcatcactaa ttaccttttg caatccttca acataagtag
    25261 actgtaactt agataattga cgatattcta aaatataatc aatgatagga tgttcacctt
    25321 gcaattgctc taagacatct acagcagtag aatatcctgt tttcgtcttt ttaataacag
    25381 gtaattgtaa tgtctcaaac aatacaacac ctaattgctt aggagaattt atattaaaat
    25441 cttcaccagc tgcatcatgg atatttcgaa tcaagacgtc taatttttct tgaatttctt
    25501 tttccatttc ttctaaatca tgaacatctg taaatatacc aatttcttcc atttcactta
    25561 aaatcttagc tagcggtagc tctaaatcag ccaagagttc tacctgattg tattcttcta
    25621 attgtttatc catatttggt ttcgcaaagt aaattgcatc agtaatagaa gcaacatatg
    25681 gatttaaaac atcatcttca ggtaccttaa atttcttacc ttttccatat atactcacat
    25741 cgtctttcac aaaactttga ccgtacaatg aaacaactga ttgaacatca ctaatcgtac
    25801 gagatggatc aattatataa ctggccaaca taatatcgaa agaaatattt tgaatatcaa
    25861 tccccaatct atgtgatgct acatatgttt ttttagcatc atatacgact tttttcgaat
    25921 tcggattttc taaccatgaa actagttcga cataattatt tatgtcatcc gcattaatta
    25981 caatatgttt ctcacctgta aataaagaga attttaaaat attatttcgc aaatagttac
    26041 caccgtctaa ttcgaaatgg atggccgctt ctttcaatga agtaaaatca atattatcaa
    26101 aagacgtttc aatttcaaat gtcttttcta ttgcatcttc aacgcttgct gattgatcaa
    26161 tgtcagccaa caattgtttg aattctaact tcttaaacaa ttcgattttt tcttgttgtt
    26221 catcttgatg agtcattaac gtatcttcaa gttttacttc aatcggacta tctacattaa
    26281 tcgttgctaa ttctttactc attaatgcat cttctttgct attttgaagt ttttctttta
    26341 actttttacc tgaaatttca tctaaatgtt catagacacc ttctactgtg tcaaattggt
    26401 ttagcaattt tattgctgtt ttctctccaa cacctgcaac acctggtata ttatcagaag
    26461 tatctcccat taatcctttc atatcaataa tttgattagg tgttaacccg ttgtattttt
    26521 ccgcaataaa gtcaggtgta taatgatcaa catcagtaac acctttttta gtgtaataaa
    26581 tggttacatt atccgttgca agttgtgtta aatctcggtc tcccgtaata ataattgtct
    26641 gaaagcccgc tttatctgct tctttactta aagttccgat aatatcatct gcctcatagt
    26701 tatctaattc ataacgttta atatgataag catctaataa ttggcgaata taaggaaatt
    26761 gctcacttag ttcaggcggc gttttctggc gtccaccttt atattcacta tatttttcat
    26821 gtctgaaagt cgttttacct gcatcaaacg ctactaaaaa atgatttggc ttttcttctt
    26881 ttaaaatctt ctctagtaac attgcaaaac catatactgc attggtatga atgcctgctt
    26941 tgtttgataa caaaggtaat gcataaaaag ctctaaaact taagctatta ccatcgatta
    27001 atactaattt attcacaatt ttaacctcca gaactaattt atatatattt tagcatattc
    27061 gactgtgatg tcgtaattaa agaaactatc tcatagagta tttatacacg acctaatttc
    27121 aaataaaaca agtgcctata tagacttagc taaaaaataa aaaagacata atcatgattg
    27181 ttcttgatga gcactccata aagtcaggtt aaaagtccaa cttttgagat gcacattatc
    27241 atttcatgat tatgtctact ttttaatatt tttatgctgc tattttaaat ttttataggt
    27301 aagaataagt tatgaatcgt ctgaaccaaa tggaatatat ttaactttgg tcattttcgg
    27361 taattcttca taattattaa accactcttg gtaattttta ttgattggtg acggatgatg
    27421 catataatgt tcaaacggca atgattccac tgtataaacc tcttgtcgtg gatgatcttt
    27481 aatttgttgt tttaataact gaactctttt ttcatgttca taatgaacat aaataaatgc
    27541 actaagatat atcacagcta aaactagtga tgcaccttta ataaaattaa cattgattga
    27601 cttatatttc cgaaattctt ttaataaaat aattaaaatg attacatgta tcgtataaac
    27661 aatcaaaaaa ttacctggtt ctattggagt aacaatgact agtgtcgacg ccgaaacaca
    27721 tattgcaata agtaaggaat ataaagtgat ttgtgtttta cgatcattaa tagacaaata
    27781 aatacctaca aatatcgaaa acgcaaagta accacataca attacgttca caaaaccaac
    27841 caatccaata tctgtatttt tatttaataa aaactgattg taaaatagta aataataaag
    27901 cggtaaagtg ataaatccta tcataatgat acgacgcttt aatattgtta aatgtacata
    27961 ccgatcactt ctaagcagta gataaataat aagcgctgca atcatactta aaattataat
    28021 ctgactgaaa atgacaccat atggaagtga cgttgatatc atttctgcaa attttgaaaa
    28081 gataccttga ttatttgaaa cttgttgata ttctgatcct tcaaataaaa ttttgcgata
    28141 atttggattg gaaaacataa tgattgtacc tatagtagca atcataaatg aaaacaataa
    28201 tttataatta agtgttcgat ttgcagtaaa ttcatacaga aaagctaaaa ttaaaatgat
    28261 acagtggaat aatgtcacat tctccataaa taattgaccg aagaagcata aaacatagaa
    28321 tagtacagta acacttaccg gctgcttttc tttatatata attgcattaa tacaataata
    28381 aataataaag agtgaaatta gtgttgatgt cgcataatta taaaatcctg caaaccagcc
    28441 atatgtatct gcataaatag cacttggtaa aattaacatt aaagaaaatg ctaacaaata
    28501 atagcttgtc cacgctttac aacgtgtaat gtgcataatc atccaaatga tgcccatact
    28561 aatgaggcca tatgaaagcc aacgtaacca gcttacatgt acagctataa tttcaaagat
    28621 atttccgata tagcgaccat taagtgatgc aaatcctact tttaaaatat cagtattata
    28681 attgctaaac cattgtaaat catcgtgcat gagtggtagt aagataccca taaaagtata
    28741 aaacaataat atcgcaatta atatcaaagt tgtcttgtgt aattgaattg ttttcacttt
    28801 gctaatcctc aaatctagtt aaattttcct caacttgtag gtcgaaaaat taattcaata
    28861 tttttaatgt atttctaatt ttcacctatg catgtttcct caatcaaatt agataaacaa
    28921 ggtatttaat attactttca acaatttatc taaatcgccc ctcgtctttt tctatgacga
    28981 atgattacac ttgtctattc aagtgtttaa attaagattt atttccgttc aaataaaact
    29041 tattactttt taattaagca tctatttcaa gcgcgttact aaaatttctt tattataata
    29101 aatatattat aaaattgctt gattcacagc aatctattac ttataactta caaatcgaca
    29161 tatacaaatg acaaaattga caaccgaatt tattccactt cattaactct aatttaatac
    29221 caatagagat ttgaacgaaa taaaaaagca gcaacaactt cgtgaaaaat acacgcgctg
    29281 ttaccacttt aatttttatt ctttataatc ttttagaata actttgaacg ttgagccttt
    29341 gccaacttga ctattcactt caatattgcc ttggtgggcc tctactatat gctttgtaat
    29401 tgataatcca agacctgtcc caccagaatc tctacttcgt gctttatcta ctctataaaa
    29461 tctttcaaaa atacgttgtt ggtcttctaa tttaatacct ataccaaaat cttgtacttc
    29521 gaaaatgaca cgaaagtcat ctcgatacac acgaacgtta atatctccat cttcataaga
    29581 atagttaatt gcattcgtta acaaattcgt gataacttga gcaattttac tttcttgtgc
    29641 tttaacaatg acatcttttt caatatcagt atgaatggaa atattttttt gattggcttg
    29701 agtcatcata ttatcaataa tacgccgcgt taaatctgat aaattcatat agtctgtatc
    29761 taactctgta tgttgctcaa tatgcgataa atctaacaga tctgttacta aagattctat
    29821 tcgatttgat tcttttaaaa ttatgtttaa aaacatgtct aatgattccg catcattttt
    29881 tgcaccatca ataagcgttt cagcaaaccc cttaattgaa gtaataggtg tttttaattc
    29941 atgtgaaaca tttgctacaa attcacgtct tagattttca agttgtttca gatttgttat
    30001 atcatgcatc acaactaaaa tcccttgcaa acttttttga gacctagtta aaatcggaac
    30061 gcatgaaata tcaaagtact tggcatggac ttggtttatt gcaacttcca attgttcata
    30121 aataggtttt tcaactttaa aactttctaa aattaattgc tcaatttcag tattaacata
    30181 gccgtgatag cctacttgtt caatattatg cgagatgttg aactgttcat aatacgcttt
    30241 atttgcaaca acgatttttc catttcgatc tatcattaaa atagcacttg gaatattttc
    30301 aatcgttgtt tttaaacggt tggattgaat tttttgctca ttattaagct tttgaaggcg
    30361 tcgtgctaaa tcattggtag acacaaaaag cgctttagtt tctacaacat tactttcagg
    30421 tacacgtatg tgataataac catttgccaa caattgtgtt gcataagtaa cttcttgaat
    30481 gggacggatt aatgtacgct taaaactacg gcttgcaaaa tacagacaaa tgagtacaac
    30541 taaacatgtc aaaataagat atttccacaa cgtccaatgc atttctgtaa tatcgttatt
    30601 gtaaccttta atccatacat gataaccgtt aaccttctta ttaaaaataa aaacgtccct
    30661 tttttgaata tagctatcac tatttgggat agctttcagt ttgccactac taaacacctt
    30721 tttatcatgc ttagttatca ataaatctat attttgctgt tttacaattt ttttaacttt
    30781 atcaatctca ttatcttgga ctaaataaat atatgatctt gcatctgttg ctagagcttg
    30841 ttcgtgtttt tctgataaaa catatgtgat ggaagcgtga ataataatgc ctaatgtaac
    30901 aaaactgata attaatatac tgcttatcaa taacattaag cggtggtgaa acttcatcat
    30961 tgttctttag gtctttccaa tttatagcct aagccacgca cagttttaat aagttgtggc
    31021 ttcttaggat tatcttctaa tttatctctt aaatgactga tatgtacatc aacaattctt
    31081 gagtctcctg caaattcata attccatacc gtatttaaca tatgctctct cgtaatgact
    31141 ctgccttgtc tttctatcaa ataaagcaag agttcaaatt ctttcggtgt tagctccaat
    31201 aactcattat gcttatatac ttcaaaataa tcgggtctaa tacgtatcga tccgatggta
    31261 atatcatcgt caatctcttc tacttctttt acaaattgag aacgtcttaa aatggctttc
    31321 acacgggcaa caacttctct aggtgaaaaa ggcttagtca tatagtcatc ggcacctaat
    31381 tctaaaccta atacccgatc aaattcatca tttttcgccg ttaacattaa tatagggact
    31441 aaatttttat ttgttcttac agtcttacat acgtcaatgc catctttttt aggtagcata
    31501 acatctaaaa taattaaatc tggctgttca ctttctacct tttctaaagc ctcatcacca
    31561 tcaaatgcga caacaacttc ataaccagct gtttctaagt tatatttaag taatgttacg
    31621 attgaatgtt cgtcatctac taccaacact ttttgcgaca tggtatgcct ccctaactta
    31681 taattatatt tacattataa ccgaactatt tataaaaata acatcctaca cattatcttt
    31741 acacattttt tacattactt tacatataaa taaaatactt cttatatttc cttctatcat
    31801 ttgcatgact tactctggga caacgaaata aattttgtaa aaatagcatt tctatcccac
    31861 tacctatgca tgagttttac tcatttattc ctaagcttat gtacatattc gctttgtcta
    31921 atgtgtaaga aacactacat aatcaatcat tggtgactct ttattatttc tatcctgttg
    31981 ccaacttcaa ttcatttaaa aaggcgaacc tagcaattaa agttgctaga ctcacctaat
    32041 tttattttgc ttattttaaa tttttaatca attcatctgc aaatgctgat gtagaaactt
    32101 cttcagcacc atccattaaa cgggcaaagt cataagtaac aacttttgaa gcaattgtat
    32161 cttcaattga atctgtaatc ttatctgccg cttcttgcca tcctaaatgt tctaacatta
    32221 atacagaact taaaattact gaagatggat tcactttatt taaacctgca tattttggag
    32281 ctgtaccatg tgttgcttca aaaatagcat gacctgtttc ataattaatg tttgcacctg
    32341 gcgcaatacc aataccacca acttgtgcag ctaaagcatc tgaaatatag tcaccattca
    32401 agttcatagt tgctacaaca tcatgctcag ctggacgagt taaaatttgt tgtaagaaaa
    32461 tgtcagcaat agaatcttta atgataatct tgccttcttt ttcagctttt tcttgagcag
    32521 cattagcagc atctctgcct tcattttcaa caatttcgtc atattgttgc caagtgaata
    32581 cttgatcacc aaattcagat aatgctaaat cgtaacccca ctgcttaaat gagccttctg
    32641 taaatttcat aatattacct ttatgaacta aagtaactga tttacggtta ttatcgatag
    32701 catattgtat agctgctcta actaatcgct cagttccttc tttagaaact ggtttaatac
    32761 caatacctga agtttctggg aatcgaatgt ttgtcgcacc catttcgttt tgtaagaagt
    32821 caattacctt tttaacttct gttgtacctt ctttaaattc aataccagca taaatgtctt
    32881 cagtattttc acggaaaata accatatcaa catcttgtgg acgtttaaca ggtgatggta
    32941 ctcctttaaa ccaacgtacc ggtcttaagc aagtaaataa atctaattct tggcgtaaag
    33001 ccacatttaa tgatctaata ccaccaccaa ttggtgttgt taaaggtcct ttaacagcaa
    33061 ttaaatattc tttaattgta tcaagtgttt cttgaggtaa ccattcacca gttgtatcaa
    33121 atgctttttg gccagctagc acttctttcc attcaatgcg tttttcgcca ttataggctt
    33181 tctcaacagc agcatctata actcggcttg ctgccttcca aatatccggt ccaattccat
    33241 caccgataat aaatgggata attggttcat taggtacgtt taatccttca gttccttgag
    33301 taattttttc tgcagtcata gttattttac ctccaaattt tacatcttca tttttaattt
    33361 gtattgatta ttttctttct tcaagcggga tatacttacg attcgtttcg ccaatatatt
    33421 tcgctctagg acgcataatt ctattatctt tatattgttc taaaatatga gcaatccatc
    33481 ctgcagaacg acttacagca aagattggcg tgaataagtc atgaggtatt tccatacagt
    33541 gataaacact cgcactataa aaatcaacat taggaattaa tcctttttct tctgccatac
    33601 gtttttccat tttcactgac atttcaaata attcttcacg accagcgtct ttcgtaattt
    33661 gacggctcat ttctcttaaa tatttcgctc taggatcacc atctttatat acacgatgac
    33721 cgaagcccat tactttatct ttattagcaa atttttcatc taagtaagca tcaacatttt
    33781 caattgaccc aatctcagat aacatcgtca taacttgttc gtttgcacca ccatgtaatg
    33841 gccctttcag agaacctacg gctgctacaa tacctgagta catatctgac aatgatgata
    33901 ccgcacaacg tgccgtaaat gcagatgcgt tcaactcatg atcagcgtgt aaaataagtg
    33961 ctttattgaa ggcttctact tctatatctg ttggtaattc cccacgtaac atatatagga
    34021 agtttgccgc ataacttaag tcaggattag gcttaagtgg ttctttatct tgtcttactc
    34081 gagcaaacgc tgtaactaat gatgctactt tagcctgtat acgcattgct ctttcataac
    34141 gattttcatc tgattcattt tcagcatcag gatcgaaatg tgcaatatat gataatgacg
    34201 tacgtaatgc tgtcattgga tgcacgtgat ctgtaacata ctcctcaaaa tgtgtataca
    34261 cacgaggatt taatgtcatg tattgattta atttcccttt tagatgagca agctcttctt
    34321 cgtttggcaa tctatagttc cataatagga aaataacttc ttcaaattgc gcattttcag
    34381 ctagatcatc aatatcatag ccggcataag tcaattgact ttcaataatt gaacttattt
    34441 tagtctccgc tgcgataacc ccttctaaac ctctttgtaa ttctgccatg ataaatttcc
    34501 cctttactgt ttctttatga aatggctttc aactaatatt atagctaaac gcatttcgtt
    34561 tgtgaaatat aaaaactggc attatatgtg caacaatttt tttgtaaatc tatgtaaatg
    34621 tcacagaata gccacaaata gcaccattaa agctttttca agatgtgaat aatccatgaa
    34681 ttttacaata gaattaaatt ttcaattggt tattatatat gattataaaa accaaagtat
    34741 attaacatgg aattttacac atttttaccg aaaataattg tatatatctc aatataagtg
    34801 cataataaac atatctatat ttaagggaga tgtattatgg ctgaaaaatt acaaagggaa
    34861 ctgagcaatc gccacataca attaattgca attggcggtg caattggtac aggcttattc
    34921 ttaggtgctg gtcaaacgat tgcattaacc ggcccttcaa ttctattaac atacatcatt
    34981 ataggattta tgttatttat gtttatgcga ggtttaggag aaatcattat acagaatact
    35041 gaatttaaat ctttcgcaga tgtaaccaat acatatattg ggccttttgc aggatttgtt
    35101 accggatgga catactggtt ctgttggatt attacaggta tggctgaagt aacggctgtg
    35161 gcaaaatatg ttagcttttg gttcccagaa attccaaact ggataagtgc actattttgt
    35221 gtactgttat taatgtcatt caacctactt agcgcaagac ttttcggaga attagaattt
    35281 tggttctcta tcattaaaat agcgacaatt attggtttaa tagtagttgg tttcgtcatg
    35341 attctatttg catttaaaac tcaattcggg catgccagtt tcacaaatct atatgaacac
    35401 ggcatattcg ctaaaggtgc ttctggattc tttatgtctt tccaaatggc actattctca
    35461 tttgtaggaa ttgaaatgat tggtgttaca gctggggaaa caaaagatcc agttaaaaca
    35521 attccaaaag caattaacag tgtacccatt agaattttaa tattttacgt tggggcgtta
    35581 gcggttatca tgtctattat cccttggcag caagttgatc ctgataacag tccattcgta
    35641 aaattattcg cattgatcgg aattccgttt gctgcgggct tgattaattt tgtagtatta
    35701 accgctgctg cttcatcatg taacagtggt atattctcaa atagccgtat gcttttcggt
    35761 ttatcaagtc aacaacaagc acctccgaac ttttctaaga cgaataaata tggcgttcca
    35821 catgttgcaa tctttgcttc atcagcatta ttacttgtgg cagcattact aaactatatt
    35881 ttcccagatg cgacaaaagt atttacgtat gtgactacca tctctacagt gttattttta
    35941 gttgtatggg gtctgattat cattgcatat atcaattata gtcgtaaaaa cccagatcta
    36001 cataaaaatg ctacgtacaa actattaggt ggcaaatata tgggctactt aatatttgta
    36061 ttcttcattt ttgtgttcgg gttattattt attaatgttg atacaagacg tgcaatttat
    36121 tttattccga tttggtttat acttttagca tttatgtact taagatataa acgtatcgct
    36181 gctaaatcaa ataaataaca acaagtttta gggcttggga catta
//
LOCUS       DANJFZ010000017        34866 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.017, whole genome shotgun sequence.
ACCESSION   DANJFZ010000017 DANJFZ010000000
VERSION     DANJFZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.017"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..413
                     /locus_tag="PWN45_000690"
     CDS             <1..413
                     /locus_tag="PWN45_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0622476.1"
                     /translation="CFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVLMAIENDEKLNIK
                     KLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQGKAIKSPLAEISV
                     KVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            629..1057
                     /locus_tag="PWN45_000691"
     CDS             629..1057
                     /locus_tag="PWN45_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL0622477.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1164..2333)
                     /locus_tag="PWN45_000692"
     CDS             complement(1164..2333)
                     /locus_tag="PWN45_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDL0622478.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2703..3341
                     /locus_tag="PWN45_000693"
     CDS             2703..3341
                     /locus_tag="PWN45_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDL0622479.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            3360..3617
                     /locus_tag="PWN45_000694"
     CDS             3360..3617
                     /locus_tag="PWN45_000694"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622480.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3596..4336
                     /locus_tag="PWN45_000695"
     CDS             3596..4336
                     /locus_tag="PWN45_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0622481.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4594..5247)
                     /gene="nreC"
                     /locus_tag="PWN45_000696"
     CDS             complement(4594..5247)
                     /gene="nreC"
                     /locus_tag="PWN45_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDL0622482.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5270..6304)
                     /gene="nreB"
                     /locus_tag="PWN45_000697"
     CDS             complement(5270..6304)
                     /gene="nreB"
                     /locus_tag="PWN45_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDL0622483.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6328..6780)
                     /gene="nreA"
                     /locus_tag="PWN45_000698"
     CDS             complement(6328..6780)
                     /gene="nreA"
                     /locus_tag="PWN45_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDL0622484.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6800..7477)
                     /gene="narI"
                     /locus_tag="PWN45_000699"
     CDS             complement(6800..7477)
                     /gene="narI"
                     /locus_tag="PWN45_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDL0622485.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7470..8045)
                     /gene="narJ"
                     /locus_tag="PWN45_000700"
     CDS             complement(7470..8045)
                     /gene="narJ"
                     /locus_tag="PWN45_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDL0622486.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8038..9597)
                     /gene="narH"
                     /locus_tag="PWN45_000701"
     CDS             complement(8038..9597)
                     /gene="narH"
                     /locus_tag="PWN45_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDL0622487.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9587..13276)
                     /locus_tag="PWN45_000702"
     CDS             complement(9587..13276)
                     /locus_tag="PWN45_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDL0622488.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFRESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13585..14562)
                     /gene="cobA"
                     /locus_tag="PWN45_000703"
     CDS             complement(13585..14562)
                     /gene="cobA"
                     /locus_tag="PWN45_000703"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL0622489.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14553..14867)
                     /gene="nirD"
                     /locus_tag="PWN45_000704"
     CDS             complement(14553..14867)
                     /gene="nirD"
                     /locus_tag="PWN45_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDL0622490.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(14871..17276)
                     /locus_tag="PWN45_000705"
     CDS             complement(14871..17276)
                     /locus_tag="PWN45_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622491.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(17342..18073)
                     /locus_tag="PWN45_000706"
     CDS             complement(17342..18073)
                     /locus_tag="PWN45_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDL0622492.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18303..18839)
                     /locus_tag="PWN45_000707"
     CDS             complement(18303..18839)
                     /locus_tag="PWN45_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622493.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19072..19896)
                     /locus_tag="PWN45_000708"
     CDS             complement(19072..19896)
                     /locus_tag="PWN45_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL0622494.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20113..20295)
                     /locus_tag="PWN45_000709"
     CDS             complement(20113..20295)
                     /locus_tag="PWN45_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622495.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20379..20846)
                     /locus_tag="PWN45_000710"
     CDS             complement(20379..20846)
                     /locus_tag="PWN45_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDL0622496.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21032..22582)
                     /gene="adcA"
                     /locus_tag="PWN45_000711"
     CDS             complement(21032..22582)
                     /gene="adcA"
                     /locus_tag="PWN45_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDL0622497.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(22771..22842)
                     /locus_tag="PWN45_000712"
                     /pseudo
     CDS             complement(22771..22842)
                     /locus_tag="PWN45_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22877..23143)
                     /locus_tag="PWN45_000713"
     CDS             complement(22877..23143)
                     /locus_tag="PWN45_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0622498.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23143..23394)
                     /locus_tag="PWN45_000714"
     CDS             complement(23143..23394)
                     /locus_tag="PWN45_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0622499.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(23559..23660)
                     /locus_tag="PWN45_000715"
     CDS             complement(23559..23660)
                     /locus_tag="PWN45_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622500.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(23667..24266)
                     /locus_tag="PWN45_000716"
     CDS             complement(23667..24266)
                     /locus_tag="PWN45_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0622501.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24285..24644)
                     /locus_tag="PWN45_000717"
     CDS             complement(24285..24644)
                     /locus_tag="PWN45_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDL0622502.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            24899..26149
                     /gene="fmhA"
                     /locus_tag="PWN45_000718"
     CDS             24899..26149
                     /gene="fmhA"
                     /locus_tag="PWN45_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDL0622503.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26246..26977)
                     /locus_tag="PWN45_000719"
     CDS             complement(26246..26977)
                     /locus_tag="PWN45_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0622504.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(26974..27693)
                     /locus_tag="PWN45_000720"
     CDS             complement(26974..27693)
                     /locus_tag="PWN45_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0622505.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27674..28453)
                     /locus_tag="PWN45_000721"
     CDS             complement(27674..28453)
                     /locus_tag="PWN45_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0622506.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28574..30013)
                     /gene="mdeA"
                     /locus_tag="PWN45_000722"
     CDS             complement(28574..30013)
                     /gene="mdeA"
                     /locus_tag="PWN45_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDL0622507.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            30187..30291
                     /locus_tag="PWN45_000723"
     CDS             30187..30291
                     /locus_tag="PWN45_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0622508.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30445..31131)
                     /locus_tag="PWN45_000724"
     CDS             complement(30445..31131)
                     /locus_tag="PWN45_000724"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0622509.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(31458..32324)
                     /locus_tag="PWN45_000725"
     CDS             complement(31458..32324)
                     /locus_tag="PWN45_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0622510.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(32877..32981)
                     /locus_tag="PWN45_000726"
     CDS             complement(32877..32981)
                     /locus_tag="PWN45_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622511.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            33076..34386
                     /gene="sbi"
                     /locus_tag="PWN45_000727"
     CDS             33076..34386
                     /gene="sbi"
                     /locus_tag="PWN45_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDL0622512.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
BASE COUNT        11278 a         6689 c         4917 g        11982 t
ORIGIN      
        1 tgtgtttttt attttatgtt tcttcaaaag aaataattaa aaaatacaca aactatctta
       61 aggaatatga tttaacctat actggttaca ttgttttaat ggcgattgaa aatgatgaaa
      121 aacttaacat caaaaaatta ggtgaacgtg tgttcttaga ttctggaaca ctgacaccat
      181 tactaaagaa attagaaaag aaagattacg ttgttcgaac acgtgaagag aaagatgaaa
      241 gaaacctaca aatttctcta acagaacaag gtaaagcaat aaaaagccct cttgctgaaa
      301 tttctgttaa ggtctttaat gaattcaaca tttcagaacg agaagcatca gatataatta
      361 ataatttgcg gaattttgtt tctaaaaatt ttgactattc cgacaaaaaa taactcccta
      421 tcataaggtg atgggcagaa gtatgcatcg atatgtttat tcattatctt tattgcttgt
      481 ataaatctcg atgctcagtt cttttgccct gattccttat caggcacaaa tctacccatt
      541 tattactttt ttatctcccc ttttacttta acttttttaa tactactcat ataatggctt
      601 tgtaatacaa atttctataa tggaagtgat gatattgaac ttcaatcaat tcgagaatca
      661 aaactttttt aacgctaatc caagtgatac atttaaagat ttaggtaaac aagtatttaa
      721 ttacttttca acaccttcat ttgtaacgaa tatatatgaa acagacgaat tatattactt
      781 agaagctgaa ctagcaggtg taaataaaga agatattagt atcgatttca ataataatac
      841 gctcactatt caagctacta gaagcgcaaa atacaaatct gaacaactca ttttagatga
      901 gcgtaacttc gaatcattaa tgcgtcaatt tgattttgaa gctgttgata agcaacatat
      961 tactgctagt tttgaaaatg ggttattaac cattaccttg cctaaaatca aaccaagcaa
     1021 tgaaactact tcatcaacat ctattccaat ttcatagtta aatttaaaat gaatacacta
     1081 ctaaagcctt aatgtcatgc tacgaatatt gcgcatgata ttaaggcttt gtatttaact
     1141 aaatgaatgg catgtattac atatcaaccg tttttcaatg agccatattc tttttggtat
     1201 aaatgacgca tggtaaataa tgcaatacat ccaaataccg ctaagaaaat aaatgctaaa
     1261 tgacttgatc ctgttaaatt agctacgtac gtgattacta gtggtgggaa gaatcctcct
     1321 aaaccaccca tcattgatac gataccattt gcggatcccg cttcatttaa gaagtatgat
     1381 ggtactaatt tgaagattaa gccgttacca atacctgcac aaatacttat tgttaaacag
     1441 cctacagtga ataatgcgat atggtctgaa atacctaaaa taatggcacc gataatcata
     1501 acaacaaaat caatcatcaa tactttaact gcattaaatt tatcacctaa aatgccacct
     1561 attggtctta agaatgttgc cagcgcaatg aatacacctg atcgaatacc agcatctact
     1621 ttgtcaattc caaaatgatt tactaagtag ttaggtaaga aaataccaaa tgctacaaaa
     1681 gcaccaaaag taataaaata ccaataactt aagtaatata atttgtagtt tttagataat
     1741 gttttcattt gagccattaa tggtacttta attttacgtt cttgtgtgtc accaaaaatg
     1801 aacataatta atgcaaataa agcgataata attaagtaac ttctaactgt tgtttgccaa
     1861 ccaataatac ccgctatcgg tggtgctaaa aatgaagaaa ctgctgtacc gatatttccc
     1921 ataccataaa taccatttgc tagacctact ttttctttag ggaaatattt aggaactgat
     1981 gtaacaccaa ctgagaaaat tgcaccacct acaccaagga aaaatcctga agccattaac
     2041 atacccggtg tttgtgcttg gcttaagaaa aatatcggga ataacaatac gataaaacta
     2101 gtaaagaata cccatttagc gccaacaatg tttgttaaat aaccaaatgg cacacggagc
     2161 accgatccca aaataactgg tatcgctaaa atgattgata tttgaccttc agtaacattg
     2221 acatcttgtt taataaaggg cattaatggc gcaattatac tccaagccat aaacccaacc
     2281 actaaactta atgtttgtaa tgtaagttga aagccacctt ttgttttgta catacgtttc
     2341 accttcatca aaatcattta ttttttaata gatatcaata ctctgcctat atattagagg
     2401 tcaatgttat ttaaaaatgc tttcatttat atgaaaacat tcaatataga caaagtattg
     2461 actacatata tattttatta tgattgtgaa aaagtgaaca taaggatttt cccttattta
     2521 tgaagtgatt taccctatac aatgtccctt taagatgctt ttagacactt attcgccatc
     2581 attttaatca tattatttag gaacactcac atacttttct taattttttt caattagtta
     2641 caattactgc ttgaaaatca cgttacgtta tcctttatgc tgacaacata aggaggttta
     2701 tcatgtataa aatcaatatt attagatcag attccgtcta taataatata ctcaaggcac
     2761 caataaacga ccgtgattca attttcacta aagaaatatt agtacctttt aaaaagaaat
     2821 ttgaggtaca acatatgcct atttataatg atgacaaaca gacaatgagt gcgattcaat
     2881 ttttagatgc ctttcaaatt tctcccaaag atttacgcat gtctgaccaa atgtcaatac
     2941 aatacctgaa taatgatttc tggagtaatt gcgaaaaata tttaaaagtt gctatagatc
     3001 aattttcaaa ttatagtatc tcatctcaag tttctaacta tcatttcaca gtattacttg
     3061 gagatcgcca aaaaccactt atgtatctaa ataaaaatcg cggtggtgat ggtggcatac
     3121 caggttatat tatgatttat ttagtgccga gtacaagtac aattaattct atgaaaagct
     3181 taattgcaca tgaagtaaat cataatatgc gctatcaata tattgattgg gatggcggaa
     3241 gtttgattga attgattatt gcagaaggat tagctgaaaa ctatgtagaa tcattgtatg
     3301 gtaaagcgca tattggaccg tgggtaacaa atactaattg aagtcgtgat aacgtaaaaa
     3361 ttaaaaatac tatttataat catttacatt taaagcatat atttgaatcg atgccttatc
     3421 tctatggtga tgatattaat aaacttcaag gtaggcctat cgttggctta tctcatgctg
     3481 ccgggtatgc atgtggctat cacttggtaa aatacttttt acaaaaaaca aacataccta
     3541 ttgaagttgc tacaacactt ccagcacaaa aaataataaa tgaggtgact gaattttggc
     3601 atacacatac actttaaaag atattattga aattacaggt gtaactaaaa gaactttaca
     3661 ttattacgat gaaataggat tattagttcc agataaaaat gataaaaatt atcgcgttta
     3721 taaacagcaa gacttagaaa aattacaaaa gattttaata ctcaagtctt ttgattttga
     3781 tatcgctaaa ataaaacaat acatttcgta tgataatgaa caattgcgaa agttattatc
     3841 agagcaaata agcaagttag ataaaaagat ttctgactta caattaatta gacgctctgt
     3901 atgtgaattt attaaaggac tctctctaat agataccagc attttaaaca agacactaca
     3961 gtcacaatat gataaagaag catctataaa atatggtcat acgaaagcat atcaatcatt
     4021 tattagacgt aaagacagct tacaatcgca ggatatcaga cataaattga caactatctt
     4081 caataaattt aatcatatgt ctttgagtca ttatccaatc caagattgta gtgatctcgt
     4141 atttgagtgg aaggcattta tgaacactat cgctgatttt gatgatgaaa cattatgctg
     4201 tattgctaaa acatatgaag atgatacgcg tttcaaagat tactttaatt catatgataa
     4261 tcaaaattta gcatcataca tttcagaagc tgttaattat tttttgagca atgtgaataa
     4321 gagcgacaat ttttaatcct catacagatg ctacaacact gaatacacca actaaaataa
     4381 ttgttcaaag tagaaaacta atcatttatt ctctaaatca ataactatat taaaagttat
     4441 acctttgcaa agcgaattag tataggttac cgaaaggaga aaggattagg ttccattcga
     4501 tttataaaaa aatatatttc gaaacataca aaagcgccag aagataatca tttcctctcg
     4561 cgcttttata ctgaattata ttaatttcaa actctaaaac tctaataatt tctttttcaa
     4621 tgcatattca actagctcag gtttactctt taagccaagc tttgtcataa tatgcgtctt
     4681 atgtgcttct actgttttca cagatacaaa taatttctct gcaatttctt tattcccgta
     4741 acctttggca ataagtggca atatttctag ttctcgtttt gataagattt taaaaggatc
     4801 tgttgtgttt gcagtgtcct gatttgaatt agatacaaat tcattcacta aagatgttgt
     4861 aagtttcata tctacatatg tttcaccttt atatacagtt cgaatagcta acaataattg
     4921 ttcatcaggt gcatttttca atatgtaacc tttcgcacca ttacgcaaca catggaacaa
     4981 atactcctca tcatcaaaca ttgttaatat tagtatttta gtttcaggaa aactgtcagc
     5041 aattttactc gtagcgataa gacctgactc acctggtggc atacttaaat ccattagtaa
     5101 cacatcaggt ttatattcca ttactttttg gtaagcttcg acgccatctg cagccgttgc
     5161 aacaacttcc atatcatttt gataatttaa aatcatagag aaccccgtac ggacaacagc
     5221 gtgatcatcg gcaatgacta ttttcaattt tattccccca atgtatgttt caaattggaa
     5281 tgttcaatgt aacattggta ccctcaccaa ttttcgtttc aatattgacg ctaccgctga
     5341 ctaactcagc tcgctcattc attccatata aaccgagtcc agaaccttta ggcttagaac
     5401 ttggatcaaa accatttccc gcatctatca cttctgctac caaatggcgc ccagtttgac
     5461 ggatacctac atttatttca tttacatcag cgtatttcaa cgcatttaaa atagcttctt
     5521 gcactactcg ataaacaacc gtttcaatat cactatcaaa gcgagtattt ttaatatttg
     5581 atgtatatat gatttttatt ccataatttt cttcaaactg tttaaaatat gatttaaaag
     5641 ctgcttcaag gcctagatca tccaaagaag cgggtcttaa ttcaaccgac atattacgta
     5701 tatcatcaat taatttagcg acaatatatt caatattttc tgcgtcttcc aaaagcttag
     5761 ttgtatcttc ttgatatttt aataatctca attgaacatc tacattgagc atttcttgaa
     5821 tcacactatc atgtaactct ctagaaattc gctttctttc attttcttgg gctgagattg
     5881 ttttacgcat catacgttgt tgatgcaatt tctcttgctg ttcaatttgt gatgaaacat
     5941 tttgaagcgt aaatgcatga attcccctgt cttgatcaat caactgatat gttgctgtaa
     6001 atggcatcac tttttgatct ttcgtcttca taaatacttg gaaattcgta gcttgtactt
     6061 gcatcgattc taagaaacag tctttacatg attgtacatc ataggcattt gtataccctt
     6121 cacatctgtg acaaatcgca ttcgccacag cactgtaatt atcttcttca gataatatat
     6181 ctttagcagc atcattcatt gcaataattt taccgttatc atcagcaaaa actatctttt
     6241 cgattgaatg ctcataatat tttttcaata aagtatctaa ctgtatactg tcctcattaa
     6301 tcatgactta caccctaatt catctcatta tttatcatca ttgaaaatac caaacttacg
     6361 ttgaatatca tcattatcaa atatttttgg taaaggacga ccatctcttt gaccaaataa
     6421 tagtacgcca tacacttgat tcttatacca aagcggcact gctaaaactg ctgttaatga
     6481 ttcgctcaat aaaattggat agtcaatctt ttcttcaggc cctaaagcta aaccaacatt
     6541 ggctattacc atacgctttc ctgttttcat aacagttcca gctaatccac gaccttttct
     6601 taaaataatc aatttaaatc gattattttt attacctgaa acatagtgcc attttattgg
     6661 agatgatggt ttgttagatt catagaaagc gattgccgca aaatcataac cctcttcttt
     6721 gcgtatttta tctaatgtct cttgaaatct acgatcttca attattgctt ctggtgtcaa
     6781 atcctttcac ctcttatgct tacactttat tcttacggta aataatatat ctgcgattta
     6841 tatatgtcaa aggtacactc caaacatgca ccaaacgtgt aaatggccaa caagccataa
     6901 tagtgaaacc taacaatata tgcattttaa atgcaatcgg cacaccactc atcaatgacg
     6961 catctggttt taacataaat aattgtctaa accaaattga taatgaagtt ctgtagttaa
     7021 agtctggatg ttgtatattt gttactaatg ttgcgtaaca tcccataaat acgataagta
     7081 ataataagaa atttacaaat atatccgacg ctgaacttaa tcttcgaata cttttcgtag
     7141 taacacgtct cgctgttaat aaaaacatcc ctatcaaagt tattatacca aagatgctac
     7201 caatataaac agcgcctata tgatataaat gctcagacac acccactgca tccatccatg
     7261 gtttcggtat taacaatcca actacgtgtc caaaaaacac tggaataata cctaagtgaa
     7321 ataataaact tccccacatc aacctttttc tttctattaa ttcactagat ttagctgtcc
     7381 aagaaaattt atcataacga taacgtgcaa tatgacctgc gacaaagaca actaaacata
     7441 aatacggaaa tataacccat aaaaactgat taagcatgat gtttcactcc ttttggtgat
     7501 gtcaaacata atttcaatgt ttttctaagt gcttgaatca cataggcata tggattgtta
     7561 tcttcaccaa gtgcattcgc catcacatat gttccatcct caataatcat aatgattaat
     7621 tgaatattct cttcagctct tggatcattt cgccattctg ccacttgcaa aaattgaagc
     7681 atcaacggta gataatcaga aagttcatta tctaccattt ctagtccaaa catttcatat
     7741 aataccttta atttagctaa catttgccca cgttcttttt gcgtatcaaa tttgttatac
     7801 gtcatatata atggtgcttt tttcgtaaaa tcaaatgtat ctgtataaat cgctttgatt
     7861 tctgataatg aaaatgtcat cattacattt ctaaatgcta ctaaatcttc atatgctgga
     7921 tgatctttcg aaattgtttc ttcaaaagtt tttggatgaa aagttaattt ttctggaaaa
     7981 cataactgtt gtgccatata tccaaaactt tcttgatatt ttttaaaatt atcgaaatta
     8041 atcacggaaa atccctccat agaaattctc attataaatt tcttgaccag ttttccctga
     8101 acctactgca acgccacagc cttcacagtt atctccaaaa tgctcgccgc cgtaattgta
     8161 tcctgtacta ccttgtgcgt gatacgtatc taaataggtt tctttgtgtg atgttggaat
     8221 aacaaatcga tcttcatatt tggctagtcc taataaacga tacatgtctt tagtttggcg
     8281 ctcggttata cctaatcgct ctaatcgaga cgtgtcaaat ggctgttgag taacttgaga
     8341 tctcatataa cttctcatca ttgccatacg ttgtagggct ccttttactg gctctgtatc
     8401 tcctgcagtg aaaatattag ctaagtattc aataggtaaa cgcatttctt caatggctgg
     8461 gaaaatcgca tctggatttt gagttgtatt tttaccttca aaatagctca taattgggct
     8521 aagtggtggg caataccaaa ccatcggcat cgttctaaat tcaggatgta acggaaatgc
     8581 aagtttatat tcaattgcta acttataaat tggagagttt tgtgcagctt caatccaatc
     8641 gtaaccaata ccatcttttt cagcttgagc aatgacttct tcgtcaaatg ggtttaagaa
     8701 tatatctaat tgtttttcat ataaatcttt ctcgtctact gctgaagctg cttcatgaac
     8761 tcgatctgca tcatataata aaacacctaa gtaacgcata cgtcctgtac aagtttcaga
     8821 gcataccgta ggcatacccg cctcgattct cgggaaacag aaagtacact tttcagcttt
     8881 gttcgttttc caattgaagt aaactttctt atatggacaa cctgtcatac agtaacgcca
     8941 tccacgacat gcgtcttggt caactaatac aatgccatct tcatcacgtt tatacatagc
     9001 acctgaagga cacgatgcaa cgcaacttgg attcaagcaa tgttcacata aacgtggtaa
     9061 atacatcata aaagtttcgt caaattggaa tttaatatct tcttctattt tttggatgtt
     9121 aggatctttt ggacctgtaa catgaccacc tgctaagtca tcttcccagt taggtcccca
     9181 ttcaatttca atgttatccc ccgtaatttc tgaatacgct ctagcaactg gcgaatgctt
     9241 ccctgatttc gcagttgtta aatgttcata attatagttc catggctcat aataatcttt
     9301 aattaatggc atatctgggt tataaaaaat tttacctaaa gcaatttttg aaattctact
     9361 tccagatttt aattcaagtt tccctttacg atttagtacc caaccacctt tgtagtgttc
     9421 ttggtcttcc caacgtttcg gataccctac acctggcttc gtttctacgt tgttgaacca
     9481 catgtactca gcacctggac gatttgtcca agtgttttta catgtcacac tacacgtatg
     9541 gcatcctatg catttatcta aatttaatac catcgcaact tgcgctttaa tcttcaagcc
     9601 aattaacctc cttcatcttt ctaactgcta catataaatc cctttggttc ccaattggtc
     9661 cataataatt aaagtgataa ctaatttgtg cgtatcctcc gactagttgt gttggtttca
     9721 aatggattct agtcggcgcg ttgtgtgaac caccacgtgt atctgtaatt tctgacccag
     9781 gcgtttgaat atgtttatct tgtgcatgat acataaacat tgtaccttta ggcatacgat
     9841 gcgaaataac tgctcttgcc gttacaacac cattacggtt atacacttct agccaatcat
     9901 tatcttggat atcgtgtttt tcagcatctt catttgatat ccaaaccgtt ggaccacctc
     9961 taaatagtgt caacatatgc ttattatctt gatacattga gtgtatattc cattttccat
    10021 gaggcgttaa ataacgcagt accaaagcat ctgtaccacc tttaattttc ttatctctat
    10081 tcccaaatac cattggcggc aatgtcggtt tatatactgg taagctctcc ctaaattgtt
    10141 ggaaaacttc gtgatccaca taataacttt gacgtcctgt taatgttcta aaaggtacta
    10201 gacgttctat attcgttgta aatggtgaat atcgtcgacc ttgtttattt gaacctggga
    10261 atactgctgt cggtattact tctcgtggtt gtgaagttat atttaaaaac gaaattttct
    10321 cagcagcgcg ttcgctagaa atatctttta acggcattcc agtttgttct tcgagatctt
    10381 catatgattt ttgtgataat ttaccattcg tagcagatga aatacttagt attgcatcag
    10441 ctacattacg tgctgtatca atacgtggac gattcgctct cacagaatca tcatttgtat
    10501 cactccacgt acctaacata ctttttaatt cttcatattg ttcactgaca ccgaaactta
    10561 caccatgtgc tccaactttc cctttttcaa gtacaggacc aagcgtgaca tatttgtcgt
    10621 aaattttagt gtagtcgcgt tctacaattg caaagttagg cattgtacgt ccaggtaccg
    10681 cttcaatttc acccttcgac caatctttca ctacgccgta tggtgttgaa atttcttgct
    10741 ttgtatcatg actaagtgga gttgtcacaa catctttaaa cgttccaggt aaatagtctt
    10801 ttgccatttc tgaaaatgct tttgccaacg ttttataaat atcccagtct gaacgcgatt
    10861 cccataacgg atcaatggca ggattgaaag gatgtacata tggatgcata tccgttgatg
    10921 ataaatcatg tttttcatac caagtcgctg ccggcaaaac aatgtcagaa tataacggtg
    10981 ttgccgtcat tctgaagtct aaagagacca ctaaatctaa cttacctgtt gtttcttcac
    11041 gccacgtaat ttcttctggc ttttcatctt catttggtgt agctaataac cctgattttg
    11101 tgccaagtaa atgcttcata aagtattctt gaccttttgc agaacttgaa attaagtttg
    11161 aacgccatat aaataatgat tttggatgat tctttttcaa atcaggatct tctattgcaa
    11221 attgtgtttg ttttgatttc acttcatcaa ttgcacgttg caaaatcgct tcatttgaat
    11281 ctataccttc atctttagct tcttctgcaa acaacaaact atttttatta aattgtggat
    11341 atgatggtaa ccaaccaagt ctagctgcta aaacattata atcagctgga tgttgatgct
    11401 ttaactcctc tgttttagct aatggagatt ttaaacgatc tacatttgac tcttcatatt
    11461 tccattggtc tgttgcaaaa tagaaccaac ttgttccgtt ttgcaaacgt ggtggtcctt
    11521 gccagtcttt cgcaaatgcg acagtactcc atccttcaat cggacgacat ttttcttgtc
    11581 ccacatagtg agcccaaccg ccaccgttca caccttgaca gccacataac ataactaagt
    11641 ttaagattga acgataaatc gtatctgagt taaaccaatg gttaataccc gcacccatga
    11701 taatcattga acgcccttca gtatcgatag cgttttgcgc aaattctttc gctacttgaa
    11761 tgacaacact ttgttttacg cctgaaatgg cttcttgcca agcaggtgta tattttgatt
    11821 ctgcatcgtc gtatcctttt gattctaatt tatgatcaaa acgacgcacg ccatattgac
    11881 ttgccattaa gtcaaaaatt gtagcaatac ggactttgtc accatttgct aaagtgactt
    11941 gtcgagttgg aattggacga ttgaatatcc catctccatc actatcaaag tatgggaatt
    12001 gaattgtttc taattcgtat ccaccttctg tcattgataa tgtagggtta attttagaac
    12061 catcttctgt ttctagtttt aagttccact tcttaccttc ttcccaacgt tgacccattg
    12121 tgccattagg tactactaaa ctatcgctga ttgcatcatg aataactggc ttccattcgc
    12181 cttgctctgt tgtttgacct aagtcactcg ctcttaaaaa tcgacccgct ttatatccat
    12241 tttcatcttc atccagcatg ataagaaacg gcatatctgt atattgttta gcgtaattta
    12301 taaagcgttc attaggttga ttaacataat gttcttgtaa aataacatgc gtcattgctt
    12361 gtgcaattgc agcatctgaa ccaggattcg gtgctagcca gttatctgca aatttcacat
    12421 tttctgcgta atctggtgct actgaaatga cttttgtacc tttatagcgg acttcagtca
    12481 taaaatgtgc atccggagta cgtgttaaag gtacattaga gccccacata ataatgtatg
    12541 atgcgttata ccagtcactt gattcaggca catctgtttg ctctccccaa atttgtggag
    12601 aggcaggtgg taaatctgca taccagtcat aaaaactaag catttcacca ccaagcaaat
    12661 tgatgaatcg agcacctgct gcataactaa tcattgacat cgctggaata ggtgtaaatc
    12721 ctgcgattcg atctggacca tattttttta ttgtatacag taattgtgct gcgattatct
    12781 ctgtaacgtc tttccaattt gaacgcacgt gccctcccat acctcgggct tgcttatatt
    12841 gtttggcttt gtcttcattt tcaacaatag acgcccatgc agcaacgcga ttaccattgt
    12901 tttcttctaa tgcttcagtc cataaatccc agagttttcc acgaatatat ggatatttga
    12961 ttcgaagcgg actgtattca taccaagaga atgacgcacc tcgtggacat cctctcggtt
    13021 catattcagg catatccgga ccacaacttg gatagtcagt ttgttgattt tcccaggtaa
    13081 tcacaccatt tttcacaaat actttccaag aacatgagcc tgtacagtta acaccatgtg
    13141 ttgttcttac ttctttatcg tggctccaac gttctctgta cattttttcc cattctctac
    13201 ttttactttc taggatcgac caattcccat taaatttttc tgttggctta aagaaattca
    13261 atccaaattt tcccatattt atatcctcct acgtataaaa atacgatgtg tagatgtcgt
    13321 gtttttaaat actttaaaat gcccaagact attgctttaa ttagattgta cattttttca
    13381 caaacataaa atattaggga atcacctaat tacttaagga atttccctat caataacggg
    13441 atttcattga aataatacac aatcatgtat ggtcatgctt attgccaatc taaatcgttc
    13501 aaatttggca caacgacaaa taaggcttca acacgaatat attctctcgg ttgaaacctt
    13561 acttattcat ttatttttta taaattagtg acataacact gtattagcat ctgcacgatc
    13621 ggttgaaata tatgttacat tttcttgctg cttaataaat gcatcatagt aatcatattg
    13681 cgacgaatga tatgtgccat tcgatgtatc atttgggttt agcaaacagc cataaccttc
    13741 gtcatataaa tgttcacaga gcataagggc gtcatgttta gaaccactta ctacataaaa
    13801 ttgcttcata ggatcatatg atttaggagt gttttcagta taatcaacaa cttcccctat
    13861 aatacatata cctggtttcg cctcaattga atagtgttgc aattttgaaa taatattact
    13921 taaacgcccc ttaacaacaa actcgttaaa acacgatgct tgaaagacaa tcgctatcgg
    13981 gtaatcaata tctgtgtatt gttgtatctg tgtgataatt ttccctaaac gttttacccc
    14041 catataaatt gctaacgtgc caccattcac taaggaattg acatccactt cattttcttc
    14101 tgaatcttta aagtgacctg tagaaaatgt cacactttta gcaactgtac gcattgtcaa
    14161 acctgtctgc atagtagcaa ctgctgcgct cgctgatgtc acccctggta caatttcaaa
    14221 cgcaatatga tgttcattta gtgtgtcgac ttcttcttgc acacgaccaa atatcgctgg
    14281 atcgccacct ttaagtctaa caaccttgtt atatcgacgc gctgcttcca cgatacagtc
    14341 atttattttt tcttgctgaa tatgttttgc atacggcttt ttaccaacat cgataatttc
    14401 agtagtcaaa ttcgcatatt gtaaaattaa cggattcact aatcgatcat atagaatgac
    14461 atccgcttca cgtattaaac gctcagcctt tttcgtcaaa taattcggat tacctggacc
    14521 cgcacctatc aagtaaacct tgccatattc ctctacagac atatatatac gttcccgtct
    14581 gtaacttcta cctcataaac atctacacaa ccttcatcag gttcttgaac aatacctgta
    14641 tttaaatcaa ttttttgatc gtggagcggg caaaatacat attccccact cactgtccct
    14701 tcagacaatg gtccttgttt gtgtggacag atattgtgaa tcgcatgaat tttgccactt
    14761 tctgttaaaa acaaccctac ctctttgcct ttgacaataa ccttttttcc aattaggggt
    14821 gttaattcat ctatagttgt cactttaatt ttttcttttg tttccatgta ttacaccttc
    14881 tccacttcaa aaattctacg tgcttgcgca ttgctagtta ttgcttccca aggttcagct
    14941 tcgactgctt ttttagcatc cataatgcgt tcaaatagtt cattttgtct ttctgggtca
    15001 agtaagactt cttttacatt ttcaaatcca agtcttctta accatggcgc tgttctttca
    15061 gcatatatac ctgtttcgcg ataatattgc atcaaagcac cacataattt gattacttca
    15121 tcttctgttt ctacagttgt taaaaattca gctttttcaa cttctgtacc accattacca
    15181 ccgatataga tttggaatcc attttcaact gagataatac caaaatcttt aacacctgat
    15241 tcaacacaac ttcttgggca gcctgataca cccattttga atttatgagg tgtatcgatg
    15301 tattcaaatg ttttttctaa acgaatgcca agtcgtgtcg tgtattgcgt accaaatcga
    15361 caaaactctt taccaacaca gcttttaact gagcgtgttt tcttaccata agctgatgct
    15421 gaacgcatac ctaggtcttc ccatatattt ggtaattctt cttttttaac tccatacaaa
    15481 ccaacacgtt gtgaacctgt cactttaact agtggcacat gatatttctt agccacttct
    15541 cctagacgaa tcagttggtc tgcatctgta acacccccac gcatttgagg tataacagaa
    15601 aatgtaccat cattttgaat attcgcatgg taacgttcgt tagcaaatct tgattctctt
    15661 tcatcttcat gatcatgtgg ataaaccatg tttaaataat agttgattgc tggtcgacat
    15721 tttggacatc cacctttatt tttaaagttt aaaacatgtc gaacttcttt agatgttttt
    15781 aaacctttcg ctcttatttg cgttactatt tgatcgcgtg tcaaatcagt acaaccacat
    15841 ataccagcag gttttgcggc aacaaagtca tctcctaagg tgtgctgcaa tatttgagca
    15901 atttgcggtt tacatttacc acatgaattc cccgcttttg ttttagccgt tacttcttca
    15961 actgttgtaa agccattttc cgtaatcgca tttactatag tacctttatc aacaccatta
    16021 caaccacaaa ttgtttcatc atcagccata tcagcaattg atagcgatgc ctcttctcca
    16081 cctttagtaa gcaatgatac aagtgtgtaa tcttcagtgg attcaccttt tttcatcatg
    16141 ttataaaagc gtgaaccatc atcgatatca ccatatagta ctgcaccaac tacattaccg
    16201 tcttttaaaa agattttttt atagttatta tcaacactat taaatatttc aataccttta
    16261 atttctgcat tttctacaat ttgaccagca ctatacaagt cacacccaga aacttttaat
    16321 gacgtaaatg ttgttgatcc cttgtatccg ttcgtttctt tatttgttaa atgatcagct
    16381 aatactttac cttgttcata tagtggtgca acgagtccat aaactttgcc gttatgttct
    16441 gcacattcac caactgcata tacattgcta tcacttgttt gcatcacatc attgacaaca
    16501 ataccacgat taacatctag acctgattct ttggctactt ctgtgtatgg tcgtatacct
    16561 actgccataa caactaagtc tgccggaatc tcgcgtccat cagccaattt aacaccctca
    16621 acatcatctt ctcctaagat ttcagttgtg ttggcttgca tttcaaactt cataccttgc
    16681 ttttctagat ctgctttaag catatttcca gctttacggt ctagttgcat ttccatcaac
    16741 cattcagcta aatgtaacac cgttacttcc ataccttgat ctaataaacc acgtgcacac
    16801 tctaaaccta gtaatcctcc accaattaca attgctttct ttttagtctt agcaatgttc
    16861 atcatttgtt cagtatcatc aagcgttctc caaccaatta cactcggtaa tgtcgaacct
    16921 ggaactggaa tgacgaatgc tttagaacct gtcgcaaaaa tcaatttatc gtatgatact
    16981 tcaataccat ttgcagtagt aactgattga tttgctctat ctacttcaat tacaggatca
    17041 tttgtaatta actcgatacc atgttcctca taccactcat atggattcat aattgtttct
    17101 tcaactgtca ttttattttg taaaatattt gaaagcatga tgcggttata gtttggataa
    17161 ggttctttac ctattaccgt aatatcatat aaatcgttgg cgcgctctaa tatttcttcg
    17221 attgttcgaa tgcccgccat accgttacca atcattacta gtttttgctt tgccataaaa
    17281 tatgcccctt tactccataa tatttatttc aaaaaaaggt attaattttt cgttagtgct
    17341 tttatatttt cattggaatc attaagcttt ctaatctatc gttaatgatt tgctttaaaa
    17401 ttgggtcgaa gttaattgaa ggtgtgaagt gtatatctgt attaataacc atgtcattca
    17461 tttgctgctt cactttgtta acaagtcttc cgtcatataa aaataatggt acgacaatca
    17521 atttttgata ccgtttcgag atgctttcta aatcatgtgt aaaactaatc tctccatata
    17581 gcgttctcgc atatgtcggc ttgctaattt gcaaattttg agcgcatatt tgtaactctt
    17641 cgtgtgcctt agtaaacttt ccattaatat tgccgtgtgc aacaaccata actccaactt
    17701 gttgttcgtc acctgctaat gcgtcacaaa tacgttgttc aattaatcgt ctcattaaag
    17761 gatgtgtgcc aagtggctcg cttacttcta cctttatgtc tggataccgt cgtttcattt
    17821 catgaacgat attcggtata tccttgagat aatgcattgc actaaagatt agcaatggta
    17881 caattttaaa atggtcaacc ccactttgaa tcaacgtcgt cattaccgtc tctaaatcct
    17941 gatgctcact ttctaaaaac gcaatatcat agtgatgtat atcatctttt actaattcag
    18001 aaataaatgc ttctaacgct tgattctgtc gtccgtgcct catgccatgt gcaacaatga
    18061 tattcccatt cacatttacc aaccctttca cacgtattgt ataccaaatc attttgtttt
    18121 tgtgaaaaga atcacattat aatgtaaaat cagggaattc cctgatgcct gtagtcatgc
    18181 atattcctta tacattttcc ctttttgtta aatcaaaaaa agcgaccgat atatgaatcc
    18241 ctactcaaca tttatttgag caagcattaa tatatcggtc gcttgtagtg tatattatta
    18301 tcttaaaatg gtggttggcc taatattgtt tcgtcaaagc gctcgggtat caatactttg
    18361 cgcatgatca cacctaaatc gccatcatca ttttcatgtt cgctgtatat ttcataacct
    18421 cttttttcat aaattttaag taaccacgga tgcaatcttg cagatgtacc taaagtaact
    18481 gccgctgact ttaacgtatc tcgcaaaaat gcttcttcaa cataagtaag taattggcta
    18541 ccatagcctt tcccttcata ctcaggattt gtcgcaaacc accagacaaa aggataaccc
    18601 gaaatacttt tcacacttcc ccaaggatat ctaaccgtaa tcgtagatat aatttcatca
    18661 tcaattgtca tgacaaatgt agtattttta tctatatttt ctttaacagc atctaaatta
    18721 gcattaaccg aaggccaatc aatacctagt tctcttagag gcgtaaatgc ttcatgcatg
    18781 agttgttgca atttttctgc atcttgttca cttgcgagtc gaatcatcgt ttttgtcata
    18841 ttaatcccca ctctttttta aatgatttaa ccatatttta tttttaaaat aaatatccat
    18901 caaagtgtat caataaattt atcacatgtc agaaagtatg cttcatctga atacaccaat
    18961 actctcatga aacttattaa aaattactct ctcaacgtaa aaaaccattc aaattcatga
    19021 atggtttgga agaatgattc attgttacgc tatttaatca ctacatctta attattgttg
    19081 ctctaaacga ttacgcttac catttaagaa agcataaacg agacctacaa aaataccgcc
    19141 accgacaaag ttacctaaga aagcaaaaac gatatttttt aaaacgtgta accatgaaac
    19201 tgcatcaagg ttaaagaata ccatacctgc atatagacct gcattgaaca caacgtgctc
    19261 atatcccatg tatacaaaga ccacgacacc acaagctatg aagaatgcct ttgttaagcc
    19321 gcctttgaat tgcatagaga tgaaaatacc aatattaata aagaagttac agaaaatacc
    19381 ttttgtaaaa atattcaacc atgttgaatc aacagtcttt ttctgaacta aagctgttaa
    19441 agcttgtgtc atttcaggtg tcatgacatg cgcaaatttc attaagaaaa ataacacaaa
    19501 cccacctaaa atatttccta agaaacaata taataaaatc caagtcatct tcttaacaga
    19561 aacgacttta taataccagc ctactgtaaa gtacatgaag ttacttgtta ataattcaga
    19621 gttagttaat acaactaaaa tcaaacctaa actaaacgca atggctccca ttaaattgat
    19681 aagtccatct acgtgagtac tcgcaaactg cgtctttatt ccgaacataa atacagtaac
    19741 gattgagagt aaaaatccgg ccatcattgc tttaagcata tatcggccag gtgtttgctg
    19801 taccattact tgcttcattt gtactgaaga tgatattgaa tcaacaattg cgcctgttga
    19861 atatgtatct tcaactgttt tcttattttc tatcacagta cttcccccat ttactttgat
    19921 caaataaaat ttactttcta attatattag taaatcaatg taattgatag attaaatttc
    19981 agaaaaatct ttttattctc tcgaaaatgt agttttcttt cataaaaaca gcgagataaa
    20041 tcgcgtgatt ggctaatatc tttatgattt aagatattac gacgctcatt ttatctcgct
    20101 ttatgtaaag tattacttat tcttttttaa atttgaaaag taatgtaaac catatgcaaa
    20161 tacactgtaa ccaattgcag tacgtaaaat aaaccttata actttattag gtacacattt
    20221 tgacgttact gaaatagaag atagtagtgt tgtgaaaata tataatatta aaactttgat
    20281 ataacgttct accatcgaat catccctcat ttttataata ttacaagatt attataaacc
    20341 gaatgaaaca aaaaagtttt tacatatttt caacaacttt atttcaataa agcatcaagt
    20401 tgatctaaag tttgattcat accttgttca acacccatgt ttatgacttg ttgagcggct
    20461 tcttttgttg gaaataccga tgttgatgtc actgtcgttt tcgttgtatt gctcttggaa
    20521 aatgacaaag taattttcat gcttggcatt tttgtatctt ttgcaccttg aggtgtcgca
    20581 aatgagtcaa tatattcaat taaatatgga cgcttaaccg ttttatattc tgctaatgta
    20641 tagctggtca ttgtaggtgt tttaatcgca taaaatgcat caccgcctga aacagcatta
    20701 aagcgaaaca ctttggtact agcgtctttt ggatgaaacc atttttcaaa taatgctttt
    20761 gtcgtatagg catcaaacac cttttcaatc ggtgcttcta tcgttcttga aaaaataatt
    20821 ttattatctt caactttaat cgtcatcttc ccactcccta tcttttatat ttactcatct
    20881 taacatgcat tgagtaacat atgttaccaa atcatttgtt ataaactata gtaatattaa
    20941 tcattccctc tgttgaaatt gataactacg aaataaaaaa cacactctat tcagctatta
    21001 acagttgagt agagtgtgca aacctgttga attaatgcgc taacatttct tctttgattt
    21061 ggtctttatt taattttgaa ggataatatg ttggccagtt atccatttct tttaataacg
    21121 cgtcattatc attacccata aagatatgga agtgttctgc ctttttaggt gcaatgttgt
    21181 gatcgctaaa ttggatgaat ttcggtaagt ctttatcatt accatcgacc aatttaaaca
    21241 taaatctcac gccacggtta cctttaggat atttcaatgt tttcttacca ttgtattcat
    21301 atttaccagt gtgtttctta ccatctttag taaatgtaat ttcatttcct tttatatcaa
    21361 tgttagtaat atcagtttta tatcctttgt cataataagc ttttaaatct ttagcagatt
    21421 tcttcggatc attttcagct ttatgttcca tcacttcatc aagcgtaccg tcttttaagt
    21481 aagggtaaac agattgccat tcaccagcat aatcgcttaa ttcacggtct ttaacttgct
    21541 catctttaaa atacccatca gaaattgctt tgtcatgttt actttcagct ttgtcgtctt
    21601 tcactttaac accactgtct aaagctttac cgatattttc aatattagat ttcattaatg
    21661 attgatacgt aatattatct tttttctgtt gttctttatt taaagactcc atgttgtaga
    21721 attttaaagg cttcgcatct gtttctttac gaattgtttc agtcacttta ttcgcaacat
    21781 tatcttcgta aagaatatat ttagcattgc tatctctaat ttctttaaca attttagtta
    21841 attctttttg tgatggatct tcagcattca tgttttgaat acctttttga acaaaaccat
    21901 aacaatcagc taagtatcca attgattcat gtgaaatgaa tactgcatta ccttgcttat
    21961 cttttgtaac ttgcttcata tcgttatcaa ttttcttaag atcgtcgttt aattttttgt
    22021 agtttttctc atagtcatct ttatgttttg gatctttttt cactaattca tctttaattt
    22081 ctttagcgaa agtttggtta attttaggat ctaaccatac gtgtggatca tatccaccat
    22141 gatgatgatg atgttcttct ttctcatgat catgtccctc atgttcatgc tcttcaccat
    22201 gctcgtgttg atcagttaaa agctttgctt tatctaattt atcctctaaa gacagttttt
    22261 tatctttatc tttgatagta gatgcaactt tcttagcaac cggatctaaa ttatcccctg
    22321 tatacataaa caagtctgac ttgcttgcac ttaatatatc tttttgtgtt ggttcatagc
    22381 tatgtaaatc tgtccctgct ggatagattg atgatacctt cacgtgtttt ccaccaattt
    22441 gctctgcaaa tgattgcaat ggataaactg tcgttttaat tgttaccttg ccatctttat
    22501 cttttccatc atcattccca catgcggcta atgataaagt tacggctggt acaagaagta
    22561 acatacctaa tttctttttc atagtcaccc tcctaaatag taatctttac gttttaaaat
    22621 tatatactca tgctacttaa aatacaagca ctgtttttat aaataatata aagtcgaatc
    22681 atactttagg tatttaccac ttttcgtttt atctaataca tgactattgt acaaatatga
    22741 tacagaaaac tttatagtat ttacgaacgt cataatataa gaaaaaaaga caatctctat
    22801 catattgcta atagaaattg ccttttcata ttgtcctaaa ataacttacc ctatttttat
    22861 agtgtgactt ttagatttaa taatggtatt tacatgatgc tatttttata tgattgtcat
    22921 ctactgtata aaccaatcta tgttcgtgat tgatacgtct actataatac ccagtcagat
    22981 tcgattttaa cttttcaggc ttacctatac cttccaatgc accatttctg tcaatacttt
    23041 taagtaactc attaatcttc ttcaccattt ttttatcgtt ctgctgaaaa tacttataat
    23101 cttcaaaggc ttgaggcgaa aacgtaatat ttaacctagc cattatacat ctatatcttt
    23161 cgttatagtt ttcccacgtt ctagatctgc aattgattga gctaagtgtt cagcattatt
    23221 tgggttctgt tggaggtaaa gtgtttccat catggagtta taatctgatt ctgacatgat
    23281 tactacgttt ttatcatcag tagatgttac agttaccata acactatcat catttacttt
    23341 tgtcataagt gcctttaaat tctgtcgagc gtatgaataa tttttaataa tcatatttat
    23401 cgcctccttt taacacacct caattgtaca aatatctgta caataaaaca agcgacacag
    23461 aaaaaaacta ctaatcaaga tttcgcacgg ggaagaatca atacctatcc tgtactgatt
    23521 ccctactaaa caatgtttac atgataattt ccacctattt agacttcata cattaaaaag
    23581 acaatctcta tcatatcgct aatagaaatt gccttttcgt attgtcctaa taaattggta
    23641 tcacttactg atctcaacaa ttaagactat ttgattttat cttttaataa cttctcataa
    23701 ctttcataat catatggatc ttcaactttc tcgccattaa taaatgcagt tggtgtcgtt
    23761 tttatatgat tatctttcgc tattttttta tctttctctg cagctttcca agacttgcta
    23821 tcttttgtct tgtaatcttt tataatttta ttttccgtct ctttatctaa atgcagttgt
    23881 ttaatatgtt tatctaatag ttcttttgtt aaccattctt tattttcatc ttgctgggca
    23941 gcaaataatt gcttttgaaa atctaaaaat gatttaggtg catacatcaa tactgcatga
    24001 ctcgcacgcg aaccaacaat tgagtcttta cctaagaaag ctaaattgac aaattggtat
    24061 tccactttgt gattatctat ataattttta cgcaactttg gcatgacttt ttcatctaat
    24121 tctttacaat aaggacattt atagtcgcca tatacgacaa ctaatggttt gccgtttttc
    24181 gaagatgtcg ttgctgattc ttttttaccg caagctgtta aaattaacat gctcactata
    24241 aataatgtta gtaatttttt agtcataata actcctattc atatctagtt cgtcatattt
    24301 tcttcatgat aatccgcatc atgttgttga taatatcgtc tcgaatcaaa atcttgctta
    24361 tatgatacac gtttatcact aaaatcatat gcataataat gcacttcatc attactcaaa
    24421 ggtttataag ctaaaatgat aattttatcc ttatcataga cataaatatt agcatctttg
    24481 cgttcaaaat gatcaacacg atcaccgttg ccttttttgg caagttgttc ttgtttttga
    24541 ttttgaattt taactgcttc atctatttct ttcacatatt tatcattacc acatcctgat
    24601 ataaagacta gtaataataa taccgtcgcc acaaatcgct tcaatgctcc cactcctact
    24661 atcaacttcc aactaacttt caatataatt aatactatac accgtcaaac attaatgcgc
    24721 tacaactact accaatgcat ttaatataaa tatccaaaga aataacagtt atgtatagtc
    24781 aaagaaaaat atatttcatt ttctatgtgt atttgtaatc agtttttgat aaaataaaaa
    24841 aatagcatta ttaataaatt caaaaattat agttcaattc agagagggat gggataaaat
    24901 gaactttgta acgttgactt cggatgagtt caatgcgttt acaacaaagc atttttcaca
    24961 ttacacacaa tcagctattc attacaatca tagagttgat ttaaaaggcg atgtgcatct
    25021 tgtaggggtt aaagatgaca atggtcaagt gattgcagga tgcttattga cagaagcacg
    25081 cacacttaaa tttttcaaat atttttatac acatcgcggg ccagtgatgg attatacaaa
    25141 tcaatcatta gtagcatttt tctttaaagc attaacgtca tatttaaaga aacacaattg
    25201 tttatatgtc cttgtagatc catatttaat tgaaaattta cgcaatgcag acggtgaaat
    25261 tgttaaatct tatgataacc gagcatttgt tagaacaatg gataaattag gttataaaca
    25321 ccaaggtttc cctgtaggtt atgattcaat gagccaaatc cgttggctgt cagtgttaga
    25381 tttaaaagat aagactgaag accaactttt aaaagaaatg gattatcaaa cgagacgtaa
    25441 tattaaaaaa acatatgata ttggtgtcaa aactaaaacg ttaacgattg atgaaacgca
    25501 aacttttttc gacttattcc atatggctga ggaaaagcac ggtttcaaat tccgtgagtt
    25561 accatacttt gaagaaatgc aaaagttata cgatgaccac gccatgttaa agttggcgta
    25621 tattgattta aacgagtatt taaaaacgtt acaattaaag caacaacaat taacagctga
    25681 actctcaggt gttgaagaag cattggaaga aagtcctaat tcaaagaaaa ataaaacgaa
    25741 acgcacacag ctagaacaac agctaaatag caataagcgt aaaatcgaca atacaataga
    25801 acaaattgaa caagatggtg cagtcttaaa cttagcttct gctttattta tctacaatga
    25861 gcatgaagtt tattacttat ctagtggttc aaaccctaaa tataatgctt atatgggtgc
    25921 ctaccatttg cagtgggaaa tgattaagtt tgctaaagcg catcatattg atcgctataa
    25981 tttttatggt attaccggtg acttttcaga gtcatcagaa gactatggcg tccaacaatt
    26041 caaaaaaggt tttaacgcac atgtagaaga gtatatcggt gactttatta agcctatcaa
    26101 accattacta tataaggtac aaacgtactt aaatcataaa cgtcgttaaa atatcgctcc
    26161 aagattatga caacataaca aagaaccacg catcatgtat atggcttcat cgccatacta
    26221 aaacatgata cgtggttcta tttgattatt cttcatttat aacatttaag aaacgtctta
    26281 attcttctgt tttcggacga ttgaacatct cttctggagc cccttgttct ccgatcatgc
    26341 cttcatgaat aaatacaatg ttattagata cttctttagc aaaacgcatt tcatgtgtca
    26401 caatgaccat tgtcatgcct tcattagcca aatctttaat aacctttaaa acatcattca
    26461 caagttcagg atcaagtgca gatgttggtt catcaaacaa catcacttta gggttcattg
    26521 ctagtgctct tgcaatagca acacgttgtt gttgaccacc tgataatgca tgtggacgtt
    26581 gatctttgac atgtgttaaa ccaactttct caagtaactc aagtgatttc ccacgtgcct
    26641 catccttttt caacttttta actgtgataa gaccttccat tacattttct aatgccgtct
    26701 tatgcggaaa aaggttataa ctttgaaata ccataccaga ctgtttacga acttctattt
    26761 gtgatttttt atctttagat gtatatgttt tgccgttaac ataaactgta ccttcagttg
    26821 gaatttctaa tgcattaatc atacgtaaca atgttgtttt acctgaacca gatcgaccga
    26881 ttaaggttac aacctcacct tgttcaacag ataaatcaat acctttgatg acttcaacat
    26941 cattaaatga tttatggata ttgttcaatt gaatcatgag cgataccctc tttcaatata
    27001 agattcatag ataccttgga taattgaaat aataaagcat accacccagt acattagcgc
    27061 aactaacaca taaattgtta aatattcata tgttgtagac gcaacttctt gagcttttct
    27121 aaacatttca gccactaaaa taaatcctaa taatgatgta tctttgatta aacttaaaaa
    27181 tgtattacct agtgcaggaa tcgacactcg aattgcctgc ggtaaaatga tacgttgtat
    27241 cgtttgacca tacgtcatac cgatggaata tgcagcttct gtttgtcctt tcggtataga
    27301 aataataccg ccacgaataa tttccgaagc atacgcacct acatttaatg ataaaccaat
    27361 aatagcagcc actacaggtg ccaatgtcca ttggttgtca gcgtcatttg ttaataatct
    27421 acctaattct ggaataccat aaaatatgat aaatagttgt actatcattg gtgttcctcg
    27481 aataatagat acatagacac gtgaaatact tcttaaaatt ttactcgttg aaattcgcat
    27541 taatgctgta aataatgcaa taatcaatcc taaaacaaat gtaactaatg taataggaat
    27601 tgaatatttg accaaacctt ctagcatagg tccgaaagct tgttttgcag catctagcgc
    27661 atgttgttgt tcgctattta gatttagaaa catcttgacc aaaccatttc ttacctattt
    27721 tagctaattc accgttttcc tcgatttttt tcaagccatc attgaatttt tgaactgttt
    27781 catcatctgc ttttttagaa aatgcaaatg cagatctact ttgttcagca ttacctttga
    27841 ttgctttgat cttagcatta ggtttttgtt ttttataatc caagtatgac agactatcat
    27901 taaatgtacc atcaacacgc ttagacaata ataaatccat tgattggtta aagccatcaa
    27961 cttttgtaat atcagcacct ttatccttag ctaatttacc ataattagat gtgaatgttt
    28021 gtgctaactt cttaccttta acatcatcaa aatctttaat atctttttca ttttcacgaa
    28081 taactaaaac agcacttgag aatgtgtaag gcttagaaaa tttatatttc ttttctctat
    28141 caggattaat acctacttgg ttcgcgatta catcaaaacg ccctgcgtct aaacctgcaa
    28201 acatagaatc ccaagaagtt tcattaaatt taagttttaa accttcttct ttagccactg
    28261 ctttaataac atcaatatcg taaccagtta atttaccatc tttatcgtgg aaactaaatg
    28321 gtgcataagt cccctcagtc ccaacattaa ttgtcttgct atctttacta gcttccttgt
    28381 ctttcgacga attgtttccg catgcagcca atatgaaaac gaaagctatc atcacaaata
    28441 aaagtctttt catagaatat atctccttat tcttattatt ctaatcggaa taaaaatatt
    28501 ttaatctgaa atgccggata agtcaatatg ttgaactttt gctaaaaagc tttatatagc
    28561 tagcaactta tatttattga cgagaatcaa cttctttttt cttaccttgt aacataaatg
    28621 aacaaaagaa actaacaact gtaatcaata atgcaaataa aaatccagcg tgataaccat
    28681 gcaacatcgt atcaacttta atttgattca tcatatcaat tttgttcatg ccattatagt
    28741 ctgacatagt tggagaaaag tttttgcttg catgggataa aatagtaatt aatgcagctg
    28801 ttccgataga accagcaatt tgttgtactg tgtttgtcat agatgaacca tgtgcattca
    28861 tttctggctt taattgattc atcgtatggg tcattactgg aattaatcct aacgcgatac
    28921 caaccattcg aatcgcataa actgttgcca acatgattgt tgatgtttgt tcatccatta
    28981 caacaaaata agccgtagta ataacaacaa tcgccatacc tactaaagca agatttctag
    29041 gaccaaactt ttcatataaa gcacccgaag tcatcgacat aaatgccata acaattgctc
    29101 ctggtaataa agttaatcca gaatctaaag ctgaacgatg caataaattt tgcacaaaga
    29161 taggtaaaat cgtttccgaa ccaatcatcg ataccattgt gacacccata ataatgatac
    29221 caactgtaaa ttgtttattt ttaaatacag caaaatttaa taatggtgtt gatagccgat
    29281 attgacgtaa tacaaataat gcgactaaaa caacgcctcc aatcatagat actaatacga
    29341 taggattatc aaaacctttt tctgaaattg aactcgttcc atacaataac ccgccgaaac
    29401 ctagtacaga aaaaatgaca gaaataaaat caataggtac tttaattgta gttccaacat
    29461 tttttatcgt tttaaatcca attaaaaatg tcacagctgc aattggagcg acaacatgga
    29521 aaagcgatct ccagttaaaa tattcaacaa ggtaacctga aagagtaggt ccaatagctg
    29581 gtgctaaacc aattaccaaa ccaaatatcc ccatatattt accgcgttca tgtggttgga
    29641 aaacatccaa aatcgttgtc atcatcaatg gcatcataat acctgcgcct aacgcctgaa
    29701 taattctagc aactaataat gttgcaaagt gcggactcaa gccgccgagt aatgtaccta
    29761 agaaaaagat agaaataccc actaaaaaca cttgtcttgt tgtataacgt tgtataacaa
    29821 gcgcggacaa aggaataaca atcccattag tcaataaaaa agctgttgtc agccattgaa
    29881 cttgtgtata tgaaatttca aaatctttca taatacttgg taaagccgtc actaatagtg
    29941 tttcatttaa taatccaaaa aatccaccca tcagcatagt aataatcatc gttaattttt
    30001 gtttcttcga catttaaagc ttccccctca ccatctaaaa aacgacacac atttttagat
    30061 atccggtaat tatacccatt ttccaaaaaa ttagcacgac tttattacaa tttctttaaa
    30121 acgtaatcat tactatttac atatgagtag ttatcggtca taatatattt acaaggaggg
    30181 atagagatga aattagattt acagactgct cgtcgtaatt taaatagccc taacattaaa
    30241 acaagaaagc gtgctttaaa gattattaag caacataaaa gagcgaaata attgttttaa
    30301 tttgactata ttgtatgtct aactatattt tcgagtatcc ctagctcgtt aatataaact
    30361 ctatacttaa ctatttcctt acattacttc acatatttaa gagctgcata tacactcact
    30421 ccttgtgtac atgcagctct ttttttataa gtagtattta tctataactt ctaaatcatc
    30481 cgttaattca taaacaagcg gtgcacctgt tttaatttca taattaatga tatcttcatc
    30541 tgacacatct tcaagatatt taatcaatgc gcgaattgaa tttccgtgtg cagaaactaa
    30601 taccgtttga ccatctagca aatattgtga aatatgatct gtccaaaatg gtatcactcg
    30661 aactaaagta tctttcagac tttcagaata aggcatcata cgtttatcta aatgattata
    30721 tcgacgatca gctaagtaag cttcacgttg ttcttcggtt tcagcaggtg gtttcacatc
    30781 ataagaacga cgccaaatat gtacttgttc ttctccaaat tcttttctag catcatcttt
    30841 atttaagcct tgcaatccac catagtggcg ttcatttaaa cgccagcttt tatatacagg
    30901 aatccattgt tgtttagatt cagttaaaat ataatgcgtt gtatctaaag cacgtgttaa
    30961 taacgatgta aaagctacat cgatggcaat gttattttct cttacttttt cacctgctct
    31021 agtcgcttca ttaatacctt gttcagataa attaacatct tcccatccag taaataagtt
    31081 tttagcattc cactcgcttt gtccatgacg acataaaatt aattttggca taacttataa
    31141 cctctttcct ttaatcttta ggacgttatt tcagggatat tttaagatga tttaatctta
    31201 gtaattttct tttcaatata ttttaaaatt caatgattaa atcaattatc actagacatt
    31261 aaatacataa atcctattcc ccattttcat tttttaattc ataaatgaat caataaccac
    31321 ctaataacaa atcatattat acacctttgt tcgctatttt tctaaggttt aaaaaatatt
    31381 tttaggtaaa cctaaaaata gatgtaataa aaacgcctcc tcagatattt atatatctat
    31441 gaagacgttt aaatacatta tagatggtct ggttctgggt gaacgtatac tgaggaaata
    31501 ccttttttgt gcaaatgatg ttcgacattg tcacaaattt gatgcgcttc tactaaggaa
    31561 aggttagcat ctacaacaat tgtgacatca ataaacacac tacttccatg gtaacgccct
    31621 ttaatacttt taacttcttg tacttcatca acttctaaaa tatcattgcg atacgcttct
    31681 aattcagttt cattgaaacc atcactcaac ataaaaattg cttctttaaa aataccaaaa
    31741 ccagtataaa cgattagtaa gcctagtaat gttgctaaaa taatatcgac aattgggaaa
    31801 ccgatttgcg taaaaattaa tcctatcgct gttccaatgc tgactaaact atccgataaa
    31861 ttatctttgg cagccgaatt taaagaacta cttttcgttc ttttcgctag tctttgattg
    31921 actgcaaata caatcaacat tacaagacca ctgattaagc tgacgataat tgttattgcg
    31981 ttaggtacaa cgtcatcttc tttgaacaaa cgaggtgcat tttgaataac tacttggata
    32041 cctacaaaca taatgacaaa tgacaccaat aatgaagaaa tattttcaga cttcaaatgg
    32101 ccataaggat gatttcgatc ggcaggttta attgaaattt tcaatccaat aataacagct
    32161 aaagaaacga taatatctgt catattgttt aatgcatcgg ctcttacagc tgcagagtta
    32221 aagacaaaac ccgtgacata cttaacaata gataagatta tatatacaat taaactcaaa
    32281 taagcaccgc gttgcgccaa tttaagattt tcattatgag acatgcgttg aaccaccttg
    32341 aattagtata gtaacaatat tatgaatgat tcattttaat tttacaacgt ttttaatttt
    32401 tataaatttt tataaaatta aactaattta ttcattcgca acccctaaaa ataattttta
    32461 gcctttctgc gaattttatg agctagaaag gcgcccaact ctccctgttt gttaactttc
    32521 gcctcgaaag tttctatgtt gggaccctat gcattagtag cgaagctatc taatgtgttc
    32581 ctttattttc tgcgaatttt atgagctaga aaggcttatg cagttgacgt tttacgtcca
    32641 actgcgttcc tccgtcttct tcaaatttat ttgttagaaa ggcacccaac tctccctgtt
    32701 tgttaacttt cgcctcgaaa gtttctatgt tagaacccta tgcatgagtt gcgaagctat
    32761 ctaatgtcgt gaactaatta tatagaagaa aaagtgcatc aatgacaaat taaatgagat
    32821 ttctactcta ccaaactctc ttcgaaagac aattttctcc tctatttatt agcaaactat
    32881 tgcatttctc catatagtac ttccttactt aaaatacgct gaatgttctg aattaaacgc
    32941 ttttttacat agttaacact agttaatcta ttagttaaca ttagttaata attagttaat
    33001 ttccatttgt attctcatgt gataaattct aaaagcatac aataaattta atatgtaaaa
    33061 agaaagggaa tacacatgaa aaataaatat atctcgaagt tgctagttgg ggcagcaaca
    33121 attacgttag ctacaatgat ttcaaatggg gaagcaaaag cgagtgaaaa cacgcaacaa
    33181 acttcaacta agcaccaaac aactcaaaac aactacgtaa cagatcaaca aaaagctttt
    33241 tatcaagtat tacatctaaa aggtatcaca gaagaacaac gtaaccaata catcaaaaca
    33301 ttacgcgaac acccagaacg tgcacaagaa gtattctctg aatcacttaa agacagcaag
    33361 aacccagacc gacgtgttgc acaacaaaac gctttttaca atgttcttaa aaatgataac
    33421 ttaactgaac aagaaaaaaa taattacatt gcacaaatta aagaaaaccc tgatagaagc
    33481 caacaagttt gggtagaatc agtacaatct tctaaagcta aagaacgtca aaatattgaa
    33541 aatgcggata aagcaattaa agatttccaa gataacaaag caccacacga taaatcagca
    33601 gcatatgaag ctaactcaaa attacctaaa gatttacgtg ataaaaacaa ccgctttgta
    33661 gaaaaagttt caattgaaaa agcaatcgtt cgtcatgatg agcgtgtgaa atcagcaaat
    33721 gatgcaatct caaaattaaa tgaaaaagat tcaattgaaa acagacgttt agcacaacgt
    33781 gaagttaaca aagcacctat ggatgtaaaa gagcatttac agaaacaatt agacgcatta
    33841 gttgctcaaa aagatgctga aaagaaagtg gcgccaaaag ttgaggctcc tcaaattcaa
    33901 tcaccacaaa ttgaaaaacc taaagtagaa tcaccaaaag ttgaagtccc tcaaattcaa
    33961 tcaccaaaag ttgaggttcc tcaatctaaa ttattaggtt actaccaatc attaaaagat
    34021 tcatttaact atggttacaa gtatttaaca gatacttata aaagctataa agaaaaatat
    34081 gatacagcaa agtactacta taatacgtac tataaataca aaggtgcgat tgatcaaaca
    34141 gtattaacag tactaggtag tggttctaaa tcttacatcc aaccattgaa agttgatgat
    34201 aaaaacggct acttagctaa atcatatgca caagtaagaa actatgtaac tgagtcaatc
    34261 aatactggta aagtattata tactttctac caaaacccaa cattagtaaa aacagctatt
    34321 aaagctcaag aaactgcatc atcaatcaaa aatacattaa gtaatttatt atcattctgg
    34381 aaataatcaa tcaaaaatat cttctctagt tttacatcat tttttaaata attttcgtaa
    34441 caaaccgtga ttaaaaagaa ccgttgattc tcaatcgaat ctacggttct tttttcattt
    34501 tccatcaatt aaatgcttct tcgctatttg tcagcccact tttttacctg caacttgtta
    34561 aataatcctt acatcgttaa cgaatagttc atcatttagt tgaatcagct caactttatt
    34621 aacttcatat tttcacaaac tattgcgcaa tccattcctt ttccactaca agcaccataa
    34681 ttaaacaaca attcaataaa ataagacttg caaagcatag ttatgtagct atataaacgc
    34741 ctgcgaccaa taaatctttt aaacataaca taatgcaaaa acatcattta acaatgctaa
    34801 aaatgtctct tcaatacatg ttgatagtaa ttaactttta acgaacagtt aattcgaaaa
    34861 cgctta
//
LOCUS       DANJFZ010000018        34402 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.018, whole genome shotgun sequence.
ACCESSION   DANJFZ010000018 DANJFZ010000000
VERSION     DANJFZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.018"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            194..514
                     /locus_tag="PWN45_000728"
     CDS             194..514
                     /locus_tag="PWN45_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL0622513.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            511..672
                     /locus_tag="PWN45_000729"
     CDS             511..672
                     /locus_tag="PWN45_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDL0622514.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            767..1027
                     /locus_tag="PWN45_000730"
     CDS             767..1027
                     /locus_tag="PWN45_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDL0622515.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            1067..1327
                     /locus_tag="PWN45_000731"
     CDS             1067..1327
                     /locus_tag="PWN45_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDL0622516.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1336..1599
                     /locus_tag="PWN45_000732"
     CDS             1336..1599
                     /locus_tag="PWN45_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622517.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1608..3551
                     /locus_tag="PWN45_000733"
     CDS             1608..3551
                     /locus_tag="PWN45_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0622518.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3553..4473
                     /locus_tag="PWN45_000734"
     CDS             3553..4473
                     /locus_tag="PWN45_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL0622519.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4554..5171
                     /locus_tag="PWN45_000735"
     CDS             4554..5171
                     /locus_tag="PWN45_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0622520.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5172..5642
                     /gene="ssb"
                     /locus_tag="PWN45_000736"
     CDS             5172..5642
                     /gene="ssb"
                     /locus_tag="PWN45_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0622521.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5672..6565
                     /locus_tag="PWN45_000737"
     CDS             5672..6565
                     /locus_tag="PWN45_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDL0622522.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            6572..6790
                     /locus_tag="PWN45_000738"
     CDS             6572..6790
                     /locus_tag="PWN45_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622523.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6799..7203
                     /locus_tag="PWN45_000739"
     CDS             6799..7203
                     /locus_tag="PWN45_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL0622524.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7216..7584
                     /locus_tag="PWN45_000740"
     CDS             7216..7584
                     /locus_tag="PWN45_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622525.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7588..7830
                     /locus_tag="PWN45_000741"
     CDS             7588..7830
                     /locus_tag="PWN45_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HDL0622526.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7839..8210
                     /locus_tag="PWN45_000742"
     CDS             7839..8210
                     /locus_tag="PWN45_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0622527.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8203..8439
                     /locus_tag="PWN45_000743"
     CDS             8203..8439
                     /locus_tag="PWN45_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDL0622528.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8429..8671
                     /locus_tag="PWN45_000744"
     CDS             8429..8671
                     /locus_tag="PWN45_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622529.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8664..9197
                     /locus_tag="PWN45_000745"
     CDS             8664..9197
                     /locus_tag="PWN45_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDL0622530.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9234..9479
                     /locus_tag="PWN45_000746"
     CDS             9234..9479
                     /locus_tag="PWN45_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622531.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9476..9682
                     /locus_tag="PWN45_000747"
     CDS             9476..9682
                     /locus_tag="PWN45_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDL0622532.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9679..10065
                     /locus_tag="PWN45_000748"
     CDS             9679..10065
                     /locus_tag="PWN45_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622533.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            10062..10211
                     /locus_tag="PWN45_000749"
     CDS             10062..10211
                     /locus_tag="PWN45_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0622534.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10211..10411
                     /locus_tag="PWN45_000750"
     CDS             10211..10411
                     /locus_tag="PWN45_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDL0622535.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10439..10855
                     /locus_tag="PWN45_000751"
     CDS             10439..10855
                     /locus_tag="PWN45_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0622536.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            11087..11386
                     /locus_tag="PWN45_000752"
     CDS             11087..11386
                     /locus_tag="PWN45_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0622537.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11516..11860
                     /locus_tag="PWN45_000753"
     CDS             11516..11860
                     /locus_tag="PWN45_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622538.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11857..13518
                     /locus_tag="PWN45_000754"
     CDS             11857..13518
                     /locus_tag="PWN45_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0622539.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            13534..14721
                     /locus_tag="PWN45_000755"
     CDS             13534..14721
                     /locus_tag="PWN45_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0622540.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14705..15442
                     /locus_tag="PWN45_000756"
     CDS             14705..15442
                     /locus_tag="PWN45_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0622541.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            15466..16611
                     /locus_tag="PWN45_000757"
     CDS             15466..16611
                     /locus_tag="PWN45_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0622542.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16631..16915
                     /locus_tag="PWN45_000758"
     CDS             16631..16915
                     /locus_tag="PWN45_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622543.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            16905..17189
                     /locus_tag="PWN45_000759"
     CDS             16905..17189
                     /locus_tag="PWN45_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL0622544.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            17173..17535
                     /locus_tag="PWN45_000760"
     CDS             17173..17535
                     /locus_tag="PWN45_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDL0622545.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            17532..17936
                     /locus_tag="PWN45_000761"
     CDS             17532..17936
                     /locus_tag="PWN45_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL0622546.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17933..18337
                     /locus_tag="PWN45_000762"
     CDS             17933..18337
                     /locus_tag="PWN45_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622547.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            18341..18985
                     /locus_tag="PWN45_000763"
     CDS             18341..18985
                     /locus_tag="PWN45_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0622548.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            19012..19251
                     /locus_tag="PWN45_000764"
     CDS             19012..19251
                     /locus_tag="PWN45_000764"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDL0622549.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            19301..19651
                     /locus_tag="PWN45_000765"
     CDS             19301..19651
                     /locus_tag="PWN45_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622550.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            19702..19839
                     /locus_tag="PWN45_000766"
     CDS             19702..19839
                     /locus_tag="PWN45_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622551.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            19896..24425
                     /locus_tag="PWN45_000767"
     CDS             19896..24425
                     /locus_tag="PWN45_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0622552.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATILAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            24422..25906
                     /locus_tag="PWN45_000768"
     CDS             24422..25906
                     /locus_tag="PWN45_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDL0622553.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            25922..29707
                     /locus_tag="PWN45_000769"
     CDS             25922..29707
                     /locus_tag="PWN45_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622554.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            29697..29849
                     /locus_tag="PWN45_000770"
     CDS             29697..29849
                     /locus_tag="PWN45_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622555.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            29896..30183
                     /locus_tag="PWN45_000771"
     CDS             29896..30183
                     /locus_tag="PWN45_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622556.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            30241..30537
                     /locus_tag="PWN45_000772"
     CDS             30241..30537
                     /locus_tag="PWN45_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDL0622557.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            30687..30863
                     /locus_tag="PWN45_000773"
     CDS             30687..30863
                     /locus_tag="PWN45_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL0622558.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(30916..31023)
                     /locus_tag="PWN45_000774"
     CDS             complement(30916..31023)
                     /locus_tag="PWN45_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622559.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            31075..31329
                     /locus_tag="PWN45_000775"
     CDS             31075..31329
                     /locus_tag="PWN45_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0622560.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            31341..32096
                     /locus_tag="PWN45_000776"
     CDS             31341..32096
                     /locus_tag="PWN45_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0622561.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            32287..32778
                     /gene="sak"
                     /locus_tag="PWN45_000777"
     CDS             32287..32778
                     /gene="sak"
                     /locus_tag="PWN45_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDL0622562.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            33428..33763
                     /locus_tag="PWN45_000778"
                     /pseudo
     CDS             33428..33763
                     /locus_tag="PWN45_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(33858..34307)
                     /locus_tag="PWN45_000779"
     CDS             complement(33858..34307)
                     /locus_tag="PWN45_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HDL0622563.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        13059 a         4805 c         6867 g         9671 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc tttcatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgcatactta tcacctcctt tcactaggag ataacaacat tatacacgaa
      181 aggaaagata gaaatgccac acattttaaa cgtaacagtt ccaatacctg aaacacatgt
      241 acttatcaca aaagatgaat atgatgagct aattggttat tcattagacc ctgtatggaa
      301 catgagtgac ttaaagaaga aattaaaaat tgcatctgat gagactatca aggacagatt
      361 actatttcat cctagatttg aaaaagaact aagagcgcaa ggaattgtgc attacccaga
      421 tgagagtttt aatcgctgga gatttaacgc aagaaagatg aataaattcg tcgatgagca
      481 tttcaatgaa atatataagg agagaataaa atgagcgaca catataaaag ctacctatta
      541 gcagtgctat gtttcacagt cttagcgatt gtgcttatgc cattgctgta cttcactaca
      601 gcgtggtcaa ttgcaggatt cgcaagcatt ggaacattca tatactacaa agaatacttt
      661 tatgaagaat aaaaaaactg ctacttgcgt caacaagtaa cagtgacaaa cgattaacaa
      721 aattaattca ttttcaatat aaaacgaaac aaggaggaag tcaaccatga ctaaaaatta
      781 taaagatatg actcaagacg aattaagggg tttattggct gaaaagaatg cagaattgtt
      841 tgatttagcg agcgaaatcg atgaagaaac tgaatttgac gttttacttt tttcaaatgt
      901 agggattagc aacggagatt ttacaccgtc gtcacattgt gtgattggga atgttgttga
      961 tattgctaat ttattgaaac gtagagctgt ttaccgagat atcgctgatg ttatcaaaat
     1021 gcgttaaaaa ttctagctat agataatgac aaggaggacg agaacaatgt attacaaaac
     1081 gggtgacgta tgtcgaaaaa taattaatgt agatggcttt gattttcaat taagagttaa
     1141 gaaacgagcg tatagcgtcg aaatggttgt tctagaccat gaaggaaatt caattgacgg
     1201 gttactagtt tctgatgaga acgatctata cacagcttta gatattttga aacaaagtgt
     1261 ttatgaatgg attgaaaata acacagatga acaggacaaa ctaatgaact tagtcatgaa
     1321 atggtaggta taagcatgag agatacagaa agaaatatat tgaatatttt taagacgtta
     1381 ttcgacgaat atactttgtc aaaccaacga gcattattgg aaattgaacg taatcatcac
     1441 ggatacttat cgattaattt cttgcactat cacgacagtt acaaaacaaa caataagctt
     1501 gtgcagatac atgaaatcaa tccagacagc catgaacgaa taaaaaattt aattatcgag
     1561 gtactaagag gtcatcggaa gattaaaaaa ggagcatgag gaaggatatg aaaataaata
     1621 aattaacaat atcgaacttt gccggaatca aagaagaaaa atttaatttt gacggtaaag
     1681 atgcaaaaat atacggcaat aatgcgactg gtaagactac aacagcaacc gcattacaat
     1741 ggttgctttt cgataaaggt ttggacggtt caaccaaatc atttaaccct gtacctttaa
     1801 acgaaaaaaa cgaagaaaat tatgagttaa ttccgactgt tttcgcagaa tttgaaatcg
     1861 acggaaaaat tacgactttt aaaaaagagt cacatcctaa atacacaata aatcaaaaga
     1921 cgaatcgcaa ggaatactca cgaagtcgaa cgaagaaaca atatatcaat gatgaatcaa
     1981 taaaagtaaa ggattataaa gctcgtattg atgaacttat tgatgaagat gtattcaagt
     2041 taattacgaa ccctcaagca tttaacttac tagattggaa gaagcgaaga agtttgttgt
     2101 ttgaaattgc taaaccaatc aatgatgagg atgtcattaa aacaaatgat gattttaaag
     2161 aactaaataa tattcttgga gatcatgaaa ttgaaacaaa gaaaaagatt cttacggaca
     2221 agataaaaca gattaacaaa gatatcaaag atattccgat acgtattaac caaacacaac
     2281 aaaataagca ggatgtacca gaattcgata atgatagata cgcaattatc aaacaagaaa
     2341 ttgagcaact tgaaaatgag cgtatagata ttcaaaacgg taaggaagaa attaatttac
     2401 gtaatcaatt agctgataaa caatcagaat tgaaacgcat agaagacaat aacagcgcaa
     2461 gtaatgagaa caaaatccat gctttaacaa atgaattaca cgttgaaaat ggaacggtag
     2521 caaaccttaa aacgagatta aagcaaaaca aacaacaaat cacgcatgaa gaaaatagac
     2581 gtaatcaatt attagaaaat cataaaggat taaaaagtga tttagaaaaa gctaaaaatc
     2641 aaaaatttga atatcttgat gacaatgtat gtagttgttg tggtcaaaag ttaccagttg
     2701 aacaagtgga tatggcaaga gaaaaagcat tgcagaaatt caatgctagc aaatcgaaag
     2761 aattagaaac aatacaaaca tctatcaatc acattatttc agaaggcaag aaaataaagc
     2821 taatcatcga gaagttagag gatgacaata ataatcttca aattaaaatc aacgaagcag
     2881 aagagcgttc agcaagaata caaaacaaaa ttaataagtt gaaaacaact cacgttgacg
     2941 ttacgcaaac tgacgaatac aaagcagtaa tgttagagat aaatgagatt aatcaaaaac
     3001 gctctaacat caggaaaact attcaagata aagtttcagg aatagatgac aaaataagcg
     3061 aacttactca agaaaaatca gaaattgaag tgtcaagatc aatcgaaaaa tcaaataaac
     3121 atctagatga tgttatttct gaattaagaa atgaagaaga cagattattg gatgaaaaag
     3181 aaaagtattc acatgacctt tatatcttaa aagaatttac aacaacaaaa gtcaaaatgc
     3241 ttactgaaaa catcaataat gaatttgaaa ttgctgaatt taagctattc aataccttag
     3301 ttaacggcga attagaagaa acatgttcca caacggttaa cggcgtcgaa tacgacagcg
     3361 gtttaaataa cgcctcaaga attaatgttg gcttagatat catcaataca ctgtcaaaac
     3421 attttaaagt tacagcgcca atatttattg ataatgctga atcagtaaca gagcttatca
     3481 aaacagaatc acaacaaatt caattgatag taaatgaaca agataaaaaa ttaagaatgg
     3541 agactatata aaatgactga aaataataaa ttacaaacta ttgaacaaca attagtacaa
     3601 gaaaagaacg tatctgacaa cgtattaaac aaagtgagag ttttagagtc acaaggcaat
     3661 ttggaattgc caaatgatta ttcaccaagt aatgccatga aacaagcatg gttacaaatc
     3721 agccaagata acaaattaat gagttgtaac gatacaagca aagcaaatgc cttattagac
     3781 atggtaacgc aaggtttaaa tccagctaaa aatcaatgct actttattcc ttacggcaac
     3841 aaaatgcagt tacaacgtag ctatcacggt aatgtaatga tgttaaaacg tgatgcaggt
     3901 gctcaagatg ttgttgctca agtgatttat aaaggcgata cattcaagca agaaatggga
     3961 gaaacaggac gtatcaaagc gattaaacac gaacaagact tctttaacat cgacaaagaa
     4021 aacattatcg gtgcgtactg cacaatcgta tttaatgatg gacgagataa ctatattgaa
     4081 gtcatgacta ttgaacaaat taaacaagca tggatgcagt catcaatgat taaagatgaa
     4141 aaagcattac aaaattctaa aacacataat aatttcaaag aagaaatggc taaaaaaaca
     4201 gttatcaata gagctgctaa acgttatatc aacacatcaa cagatagcaa tcttttcaaa
     4261 tacgcacaag aatccgaaca acgtcaacgc aaagaagtgt tggacgcaga agttgaagaa
     4321 aatgcaaatc aagaacaatt ggactttgaa caaccagttc ttgaagaagc acaatacaca
     4381 gaattagaaa atgataagcc tattgatgta tctgactttg aagaaataaa agaacctgca
     4441 acagaaaaag aaagcgaaga agagccattt taattgaaac aatagcaact ggttcaagtg
     4501 gtaactgcta cgtcttaaat gatggacgta ctacgttact acttgaggca ggtataaaat
     4561 ttgaacgtgt tcaaaagcat ttcaaatata aaacaagaca tatagcaggg tgtcttatca
     4621 cacacgaaca tggtgatcat gcaaagtata caaagcagtt tgtcgacaat ggtgtaatca
     4681 gctatatgac tgctggaaca caacaagcta tgaattttga aagtcatcgc ttatgcacga
     4741 ttaaggcaaa gcaagagctg cgaataggta catggtcaat tctaccgttt gacatcgaac
     4801 atgatgctaa cgagcctgtg gctttcttat tacaaagcac actaggttat aaggttctgt
     4861 atgttactga tacaaagtat ttgaaataca aatttaatgg cattacgcac atgatgctag
     4921 aagttaatta tatctatgaa caaatgcaag aaaacataaa aaacggcagt gtgcacagca
     4981 cattagcaaa cagaattatg gagtctcatt ttagcttaga acatgctatc ggaatgttaa
     5041 aagcaaatga tttaactaga ctcgaagaaa tacatttaat tcatttaagt agtcaaaatt
     5101 caaatgcaaa atacattaaa agtgaaatac aaaaagtgac gggcgcgccc gtttattttg
     5161 gaggtttata aatgctaaac agaacaatat tagttggtcg tttaactaga gacccagaat
     5221 taagaaccac tcaaaatggt gtaaatgtag catcattcac attagcagtt aaccgtacat
     5281 ttacaaatgc acaaggcgag cgcgaggcag actttattaa tatcatcgta tttaaaaaac
     5341 aagcagagaa cgttaataaa tacctatcta aaggatcgtt ggcgggcgta gatggtaggt
     5401 tacaaacgcg gaactatgaa aataaggaag gtcaacgtgt atacgttacg gaagttattg
     5461 ctgatagtat tcaattttta gaaccgaaaa actcaaatga cactcaacaa gatttatatc
     5521 aacaacaagt acaacaaaca cgtggacaat cgcaatattc aaataacaaa ccagtaaaag
     5581 ataatccgtt tgcgaatgca aatggtccga ttgaactaaa tgatgatgat ttaccattct
     5641 aatttaaccg gtttgaaagt gaggtgtgta tatgactggt tggataagta ttgatcgctc
     5701 aattcaaaat cattggctat ttaaagaaaa gagaacattt tcaaagtttg aagcatggat
     5761 atatttactc atggaagcga atcattcaaa ggcaaaagtg cctataggaa accaaattgt
     5821 aaccgttgaa agaggacaaa gattaacatc tattttgacc ttgtctgacc tttttaactg
     5881 gtcacgattt aaagtgaaaa ccttccttga cttactcgag agtgatggaa tgttagaagt
     5941 caaaacaaca tcaaaatata cccttataac cattgtcaat tatgactttt atcaaagtga
     6001 gcagggcagg aaccaacatc aaaacgacat caaaccaaca tcaaaacaac atcagtcaaa
     6061 catcaaccca acatcaaaac aacatcaaac caacacaaac aataatgata ataaagataa
     6121 taatgaaaag aatgtgaata atgagaagaa gaagacaacc gccttcgact tcttccaaga
     6181 taacggattc ggtttcataa cttcttacaa tttagacgat ttaaattatt atcttgattc
     6241 atttgaaaat gattcagatg aaatagttac cgcatcactt aaaatcgcta aagacagaaa
     6301 caaagttact tggggatatg ctaaaagcat tttgaataca tggcttaatg caaacttgaa
     6361 atctattgaa caagtacgtg catttgaaaa gcaacaactt gaaagcaaaa aacaaaatta
     6421 taaacctttc gttaaacaat caaaagaaaa aacacccaaa tggctcacag acagcacgag
     6481 agaaacgaaa acgccggaag tagatgaaaa ccttgagaaa gacagagaag cttttattaa
     6541 gcgtctaaat agcaaatggg agtgattgaa aatggatgca tttgataaat actatctatt
     6601 tgatcatgac ggcaacaaaa tgttttcagt tacaccacat tttaaagatg gacggcattt
     6661 agttgttgga ttaaaacaca caaaattcaa tggtcgacgt tggtacttag atgattatga
     6721 attaaaaaca cttattgata atgaacaaat ggagttagga caccaaacaa gcttatttga
     6781 atatatatga gggattacat ggagatagaa attaaattta atgaagtgtt taatgcgccg
     6841 atgggctcgc ctcgaccgcg ttttagcaca aaaggtagat atgcacacac atatatgcct
     6901 acaaaatata cagaacataa aaaatattta caaaatcaaa tgccaaagct aaatctagaa
     6961 aatgcattaa aaattgaatt agagttttac tttactccac ctaaatcatg gtcgaagaaa
     7021 aagaaaactc aagcgattgg acaattaaaa gtaactaaac cagatatcga taatttgatg
     7081 aagacagttc tagacgcttg taacaattat ttgtggaaag acgataacca aattacagaa
     7141 ataactagct caaagcgtta tggaattgag cccaaaataa tcatacgaat agaagaaata
     7201 taagaggtgg ataaaatggc gagaaaagca aggattgtaa caataaatga taaaccatat
     7261 aggtttacca aatctgaaat ggaattaata gaaagtcacg gtataaccgc tggaatggtt
     7321 tctaagagag taaaagacgg ttgggaacta catgaagcaa tggacgcacc agaaggtacg
     7381 cgtttaagcg agtacagaga aaagaaaaca atagaaagac tggaacaagc tagactcgaa
     7441 cgcaaattgg aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa gccacatttg
     7501 tttaatgtac cacagaaaca tccaagagga cgttatgcgt gctacctgat ggaaaacgac
     7561 atattcgtga aagttaagaa gtagatcatg acagataacg cacgcaaaga atacctaaat
     7621 caattctttg gatctaagag atatctgtat caggataacg aacgagtggc acatattcat
     7681 gtagtaaacg gcacttatta ctttcatggg catatcgtac caggttggca aggcgtgaaa
     7741 aagacatttg atacagctga agagcttgaa acatatataa agcaacaggg tttggaatat
     7801 gaggaacaga agcaactaac tttattttag aggaggttat gaaagtgaac tatgaaacag
     7861 gattccaaat aggcgtaatg gaagctaggt tgaagaagat gagaaaacaa cgtgatgagt
     7921 acaagaagca acgtgacgag cttattgggg atatagctaa gttaagagag cgtaacgaag
     7981 agctggagaa catgtggcgc acgcttaaaa atgaattgtt tggaagatac gaattttacc
     8041 gttttagact tagcgaacta cagattgaga gcagagcgaa caaggaagta gctatatata
     8101 gaagagctga aatcaactta agtgttatat tgtgccgaat ggacaaacta gacggaacaa
     8161 atgagttcta cgaattttta gggcaaatgg aggatgacac aaatgaataa ccgcgaacaa
     8221 atcgaacaat cagttatcag tgctagcgcg tataacggca atgacacaga gggattgcta
     8281 aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaattcg cgaagctatt
     8341 aatgcgcaat gggttgagta tccagaagac tgggcgtcag aggttttgag agaagtaaga
     8401 gagcttgaat atgaggagga acaggaaaat gaaaattaat gaatctttga agaaattaaa
     8461 agaaaaagga tacaaagaaa atgaagataa agctattttt aatttagctg atggtacgct
     8521 agaaatttac atcgaccatg acgaaaaaac aataattaca gaattccatg atttaaaggt
     8581 attcgtgtca gaagatttaa aagataaaag tatggagagc gttatgtatg aattggctgg
     8641 aattgatgag gaggacaagg aaaatgacta acatcctaca agtaaagcta ttatcagaaa
     8701 acgctagaat gccagaacga aatcataaga cggatgcagg ttatgacata ttctcagctg
     8761 aaactgtcgt acttgagcca caagaaaagg cagtgatcaa aacagatgta gctgtaagca
     8821 taccagaggg ctatgtcgga ctattaacta gccgtagtgg tgtaagtagt aaaacgcatt
     8881 tagtgattga aacaggcaag atagacgcgg gatatcacgg caatttaggg attaatatca
     8941 agaatgataa tgaaacgtta gagagtgagg atatgagtaa ctttggtcgg agtcctgctg
     9001 ggatagatgg aaagtatgcc cgactacctg taacagataa aattttatgt atgaatggta
     9061 gttatgtcat aaacaaaggc gacaaactag ctcaattggt tattgtgcct atatggacac
     9121 ctgaactaaa gcaagtggag gaattcgaga gtgtttcaga acgtggagca aaaggcttcg
     9181 gaagtagcgg agtgtaaaga catattagat cgagtcaagg aggttttggg gaagtgagcg
     9241 acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc atagcaatta
     9301 tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta atgcttgcta
     9361 ctatctttat gatatctaca tttgcttata aacatcaaaa gacgcatatc ttaatagcat
     9421 ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat gaggaatgac
     9481 acaatactta gtcacaacat tcaaagattc aacaggacgt aaacatacac acataactaa
     9541 agctaagagt aatcaaaggt ttacagttgt tgaggcagag agtaaagaag aagcgaaaga
     9601 gaagtacgag aaacaagtta aaagggatgc agttattaaa gtgggtcagt tgtttgaaaa
     9661 tataagggag tgtgggaaat gattaaaaaa cttaaaaata tggatgggtt cgacatcttt
     9721 attgttggaa tactgtcatt attcggtata accgcattgc tacttgttgt cgcattgcct
     9781 atctatacag tggctagtta ccaaaacaaa gaagtacatc aagggacaat tacagataaa
     9841 tataacaaaa gacaagataa agaggacaaa ttctatattg tattagatga taaacaagtc
     9901 atcgaaaact cagacttatt cttcaaagga aagtttgata gcgcagacat acaagctagg
     9961 ttaaaagtag gtgataaagt aaaagttaag acgattggat atagaataca ctttttaaat
    10021 ttatatccgg tcttatacga agtaaagaag gtagataaaa aatgattaag caaatactaa
    10081 gattattatt cttactagcg atgtatgagt taggtaagta tgtaactgag caagtatata
    10141 ttatgatgac ggctaatgat gatgtagagg cgccgagtga ttacgtcttt cgagcggagg
    10201 taagtgagtg atgtggatta ctatgactat tgtatttgct atattgctat tagtttgtat
    10261 cagtattaat agtgatcgtg caagggagat acaagcgctc agatatatga atgattatct
    10321 acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca ggattgaatt
    10381 aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat tacattgaat
    10441 gataaagatt gagaaacacg atatcaaaaa gcttgaagaa tacattcagc acatcgataa
    10501 ctatcgaaga gagttgaaga tgcgagaata tgaattactt gaaagtcatg aaccagataa
    10561 tgcgggagct ggcaaaagta atttgccggg taacccgatt gaacgatgtg caataaagaa
    10621 gtttagtgat aacaggtaca atacattaag aaatatagtt aacggtgtag atagattgat
    10681 agatgaaagt gatgaggata cgcttgagtt attaaggttt agatattggg attgtcctat
    10741 tggttgttat gaatgggaag atatagcaca ttactttggt acaagtaaga caagtatatt
    10801 acgtagaagg aatgcactga tcgataagtt agcaaagtat attggttatg tgtagcggac
    10861 ttttacccta tgtaagtccg cattaaaaca gtttattatg ttagtatcag attaatattt
    10921 aaagttatta aatgctaata cgacgcatga acaagaggcg catcactatg tgatgtgtct
    10981 ttttatttat gaggtatgaa catgttcaaa ctaatagtaa atacattact acacatcaag
    11041 tatagatgcg tcttgatact acttaagtta tataaggtga aacattatga tgactaaaga
    11101 cgaacgcata cgattctata agtctaaaga atggcaaaca acaagaaaaa gagtgctaga
    11161 aagagataat tatgaatgtc aacaatgtaa gagagacggc aagttaacga catatgacaa
    11221 aagcaagcgt aagtcgttgg atgtagatca tatattatcg ctagaacatc atccggagtt
    11281 tgctcatgac ttaaacaatt tagaaacact gtgtattaaa tgtcacaaca aaaaagaaaa
    11341 gagatttata aaaaaagaaa ataaatggaa agacgaaaaa tggtaaatac ccccgggtca
    11401 aaaaaatcaa aagcgatcaa aatacttggg gaacggggag gggctcgact tcgcgataat
    11461 tttaaaaatc catgtatacc ccccctctta taaccatttt aaggcaggtg atgaaatgga
    11521 gattatagtt gatgaaaact tagtgcttaa agaaaaagaa aggctgcaag tattatataa
    11581 agacatacct agcaataaat taaaagtagt tgatggttta attattcaag cagcaaggct
    11641 acgtgtaatg cttgattaca tgtgggaaga cataaaagaa aaaggtgact atgatttatt
    11701 tactcaatct gaaaaggcgc caccatatga aagggaaaga ccagtagcca aactatttaa
    11761 tgctagagat gctgcatatc aaaaaataat caaacaatta tcggatttat tgcccgaaga
    11821 gaaagaagac acagaaacgc catctgatga ttacctatga ttagtaataa atacgttgat
    11881 gaatatataa atttgtggaa acaaggaaag ataattttaa ataaagaaag aattgatctc
    11941 tttaattatc tacaaaaaca tatatattca cgagatgatg tatattttga tgaacagaaa
    12001 atcgaggatt gtatcaaatt tattgaaaaa tggtattttc caacattacc atttcaaagg
    12061 tttatcatag ctaatatatt tcttatagat aaaaatacag atgaagcttt ctttacagaa
    12121 tttgctattt tcatgggacg tggaggcggg aaaaacggtc taataagtgc tattagtgat
    12181 tttctttcta cgcccttaca cggagttaaa gaatatcaca tctccattgt tgctaatagt
    12241 gaagatcaag caaaaacatc gtttgatgaa atcagaaccg ttttaatgga taacaaacga
    12301 aataagacgg gtaaaacgcc aaaagctcct tatgaagtta gtaaagcaaa aataataaac
    12361 cgtgcaacta aatcggttat tcgatataac acatcaaaca caaaaaccaa agacggtgga
    12421 cgtgaggggt gtgttatttt tgatgaaatt cattatttct ttggtcctga aatggtaaac
    12481 gtcaaacgtg gtggattagg taaaaagaaa aatagaagaa cgttttatat aagtactgat
    12541 ggttttgtta gagagggtta tatcgatgca atgaagcaca aaattgcaag tgtattaagt
    12601 ggcaaggtta aaaatagtag attgtttgct ttttattgta agttagacga tccaaaagaa
    12661 gttgatgaca gacagacgtg ggaaaaggcg aacccaatgt tacataaacc gttatcagaa
    12721 tacgctaaaa cactgctaag tacgattgaa gaagaatata acgatttacc attcaaccgt
    12781 tcaaataagc ccgaattcat gactaagcga atgaatttgc ctgaagttga ccttgaaaaa
    12841 gtaatagcac catggaaaga aatactagcg actaatagag agataccaaa tttagataat
    12901 caaatgtgta ttggtggttt agactttgca aacattcgag attttgcaag tgtagggcta
    12961 ttattccgaa aaaacgatga ttacatttgg ttaggacatt cgtttgtaag acaagggttt
    13021 ttggatgatg tcaaattaga acctcctatt aaagaatggg aaaaaatggg attattgacc
    13081 attgtcgatg atgatgtcat tgaaattgaa tatatagttg attggttttt aaaggctaga
    13141 gaaaaatatg ggcttgaaaa agtcatagct gataattata gaactgatat tgtaagacgt
    13201 gcgtttgagg atgctggcat aaaacttgaa gtacttagaa atccaaaagc aatacatgga
    13261 ttacttgcac cacgtatcga tacaatgttt gcgaaacata acgtaatata tggagacaat
    13321 cctttgatgc gttggtttac taataatgtt gcagtaaagg ttaaacccga tggtaataaa
    13381 gaatatatta aaaaagatga aaatagaaga aaaaccgatg ggttcatggc ttttgttcac
    13441 gcattatata gagcagacga tatagtagac aaagacatgt ctaaagcgct tgatgcatta
    13501 atgagtatag atttctaata gaggaggtga gacatgagta ttctagaaaa gatatttaaa
    13561 actaggaaag atataacata tatgcttgat ttagatatga tagaagatct atcacaacaa
    13621 gcgtatgtga aacgtttagc gattgatagt tgtattgaat ttgttgcgcg agctgtcgct
    13681 caaagtcatt ttaaagtatt ggaaggtaat agaattcaaa agaatgatgt ttactacaag
    13741 ttaaatataa aaccaaatac tgacttatca agcgatagtt tttggcaaca agttatatat
    13801 aaactaattt atgataacga ggttttaatc gtagtaagtg acagcaaaga attacttatc
    13861 gcagatagct tttacagaga agagtacgct ttgtatgatg atatattcaa agatgtaacg
    13921 gttaaagatt atacttatca acgtactttc acaatgcaag aggtcatata tttaaagtac
    13981 aacaacaata aagtgacaca ctttgtagaa agtctattcg aagattacgg gaaaatattc
    14041 ggaagaatga taggtgcaca attaaaaaac tatcaaataa gagggatttt gaaatctgcc
    14101 tctagcgcat atgacgaaaa gaatatagaa aaattacaag cgttcacaaa taaattattc
    14161 aatactttta ataaaaatca actagcaatc gcgcctttga tagaaggttt tgattatgag
    14221 gaattatcta atggtggtaa gaatagtaac atgccttttt ctgaattgag tgagctaatg
    14281 agagatgcaa taaaaaatgt tgcgttgatg attggtatac ctccaggttt gatttacgga
    14341 gaaacagctg atttggaaaa gaacacgctt gtatttgaga agttctgttt aacaccttta
    14401 ttaaaaaaga ttcagaacga attaaacgcg aaactcataa cacaaagcat gtatttgaaa
    14461 gatacaagaa tagaaattgt cggtgtgaat aaaaaagacc cacttcaata tgctgaagca
    14521 attgacaaac ttgtaagttc tggttcattt acaaggaatg aggtgcggat tatgttaggt
    14581 gaagaaccat cagacaatcc tgaattagac gaatacctga ttactaaaaa ctacgaaaaa
    14641 gctaacagtg gtgaaaatga tgaaaaagaa aaagatgaaa acactttgaa aggtggtgat
    14701 gaagatgaaa gcggagatta aaggcgtcat cgtttccaac gaagataaat gggtttacga
    14761 aatgcttggt atggattcga cttgtcctaa agatgtttta acacaactag aatttagtga
    14821 tgaagatgtt gatattataa ttaactcaaa tggtggtaac ctagtagctg gtagtgaaat
    14881 atatacacat ttaagagctc ataaaggcaa agtgaatgtt cgcatcacag caatagcagc
    14941 aagtgcggca tcgcttatcg caatggctgg tgaccacatc gaaatgagtc cggttgctag
    15001 aatgatgatt cacaatcctt caagtattgc gcaaggagaa gcgaaagatc taaatcatgc
    15061 tgcagaaaca ttagaacatg ttggtcaaat aatggctgag gcatatgcgg ttagagctgg
    15121 taaaaacaaa caagaacttg tagaaatgat ggctagggaa acgtggctaa atgctgatga
    15181 agccattgaa caaggttttg cggatagtaa aatgtttgaa aacgacaata tgcaaattgt
    15241 agcaagcaat acacaagtgt tatcgaaaga tgtattaaat cgtgtaacag ctttggtaag
    15301 taaaacgcca gaggttaaca ttgatattga cgcaatagca aataaagtaa ttgaaaaaat
    15361 aaatatgaaa gaaaaggaat cagaaatcga tgttgcagat agtaaagtat cagcaaatgg
    15421 attttcaaga ttcctttttt aatacaaaaa ataggaggtc ataaaatgac tataaattta
    15481 tcggaaacat tcgcaaatgc gaaaaacgaa tttattaatg cagtaaacaa cggtgaaccg
    15541 caagaaagac aaaatgaatt gtacggtgac atgattaacc aactatttga agaaactaaa
    15601 ttacaagcaa aagcagaagc tgaaagagtt tctagtttac ctaaatcagc acaatctttg
    15661 agtgcaaacc aaagaagttt cttcatggat atcaataaaa acgttaacta taaagaagaa
    15721 aaacttttgc cagaagaaac aattgataga atttttgaag atttgacgac gaatcatccg
    15781 ttattagctg atttaggtat taaaaacgct ggtttgcgtt tgaagttctt aaaatctgaa
    15841 acttctggcg tagccgtttg gggtaaaatc tatggtgaaa ttaaaggtca attagatgct
    15901 gcgttcagtg aagaaacagc aattcaaaat aaattgacag cgtttgttgt tttaccaaaa
    15961 gatttaaatg attttggtcc tgcgtggatt gaaagatttg ttcgtgttca aatcgaagaa
    16021 gcatttgcag tggcgcttga aactgcgttc ttaaaaggta ctggtaaaga ccaaccaatc
    16081 ggcttaaacc gtcaagtaca aaaaggtgta tcggtaactg agggtgctta tccagagaaa
    16141 gaagaacaag gtacgcttac atttgctaat ccgcgcgcta cagttaatga attgacgcaa
    16201 gtgtttaaat accactcaac taacgagaaa ggtaaatcag tagcggttaa aggtaatgta
    16261 acaatggttg ttaatccgtc cgatgctttt gaggttcaag cacagtatac acatttaaat
    16321 gcaaatggcg tatatgttac tgctttacca tttaatttga atgttatcga gtctacagtc
    16381 caagaagcag gtaaggtttt aacgtacgtt aaaggtttat atgatggtta tttagctggt
    16441 ggtattaatg ttcagaaatt taaagaaaca cttgcgttag atgatatgga tttatacact
    16501 gcaaaacaat ttgcttacgg caaagcgaaa gataataaag ttgctgctgt ttggaaatta
    16561 gatttaaaag gacataagcc agctttagaa ggtaccgaag aaacactata aaattttatg
    16621 aggtgataaa atggtgaaat ttaaagttgt tagagctttt aaagacatag agcacaatca
    16681 acacaagtac aaagtagggg agttgtatcc agctgaaggg tataacaatc ctcgtgttga
    16741 attgttgaca aatcaaatca aaaataagta cgacaaagtt tatatcgtac ctttagataa
    16801 gctgacaaaa caagaattat tagaactatg cgaatcatta caaaaaaaag cgtctagttc
    16861 aatggttaaa agtgaaatcg tcgacttatt gaatggtgaa gacaatgacg attgatgatt
    16921 tgcttgtcaa atttaaatca cttgaaaaga ttgaccataa ttcagaggat gagtacttaa
    16981 agcagttgtt aaaaatgtcg tacgagcgta taaaaaatca gtgcggagtt tttgaattag
    17041 agaatttaat aggtcaagaa ttgatactta tacgcgctag atatgcttat caagatttat
    17101 tagaacactt caacgataat tacagacctg aaataataga tttttcgtta tctctaatgg
    17161 aggtatcaga agatgaagaa agtgtttaaa aaacctagaa ttacaactaa acgtttaaat
    17221 actcgtgttc atttttataa gtatactgaa aataatggtc cagaagctgg agaaaaagaa
    17281 gaaaaattat tatatagctg ttgggcgagt attgatggtg tctggttacg tgaattagaa
    17341 caagctatct caaacggaac ccaaaatgac attaaattgt atattcgtga tccgcaaggt
    17401 gattatttac ccagtgaaga acattatctt gaaattgaat caagatattt caaaaatcgt
    17461 ttgaatataa agcaagtatc accagatttg gataataaag actttattat gattcgtgga
    17521 ggatatagtt catgagtgtg aaagtgatag gtgataaagc attagaaaga gaattagaaa
    17581 atcgtttcgg aactcaagcg atgttaaaag ttcaagatag ggcgctaatt gctggtgcta
    17641 aagtaattgt tgaagaagta aaaaaacaac taaagccatc aaaagatacg ggcgcattaa
    17701 ttaatgaggt aagttttagt aaacctgaat ggataaacgg aaaacgtaca attactgttc
    17761 attggcgagg ttctaaagac cgttataaaa tcgtacattt aattgaaaat ggacacgttc
    17821 aaaaagggac aggtaaattt atcaaaccta aagctatggg tggcgttaat agagcaataa
    17881 gacaagggca aaataagtat tttgagacgc taaaaaggga gttaaaaaaa ttgtgattga
    17941 tattttgtac aaagttcatg aagtgattag tcaagacaga attattagag agcacgtaaa
    18001 tatcaataat attaagttca ataaataccc aaacgtaaaa gatacggatg tacctttcat
    18061 agttattgac gatatagacg acccaatacc tacaacttat actgacggag atgagcgcgc
    18121 atatagttac attgtacaaa tagatgtatt tgtgaagttt aacgaaaagt ataacgcgag
    18181 aatcataaga aataagatat ctaatcgtat tcaaaagtta ttatggtctg aactgaaaat
    18241 gggaaatgta tccaacggaa aacctgaata tatagaagaa tttaaaacat atagaagcac
    18301 tcgtgtctac gagggcattt tttacgagga ggaataataa atggtagtaa aacaagcaag
    18361 tgcgccgaaa gcgtatttta acattactgg tttaggtttc gctaaattaa caaaagaagg
    18421 cgcaaagcta gaatatagcg atattacaaa aactagagga ttacaaaaaa tcggtgttga
    18481 aactggagga gacttgaaaa cagcatatgc tgatggtggt ccaatcgaat cagggaacac
    18541 agacggagaa ggtaaaattt cgttacaaat gcatgctttc cctaaagaga ttcgcaaaat
    18601 agtattcaat gaagagtatg atgaatacgg tgtttataaa gaaactcaag gtaaacaaaa
    18661 caactatgta gcaatttggt tcagacaaga gcgtcgagac ggcacattta gaacggtttt
    18721 attacctaaa gtcatgttta caaatcctaa aatcgatggc gaaacggctg agaaagattg
    18781 ggatttctca agcgaagagg ttgaaggtga ggcacttttc cctttaattg ataataaaaa
    18841 gtcagtacgt aaatatatct ttgattcagc aaacatgaca aatcatagtg gaaatggtga
    18901 aaaaggcgaa gaggctttct taaagaaaat tttaggcgaa gaatatactg gaaacgtgac
    18961 agaggataac ggcgaaactt tgtaacgcaa ccggcttcat cggaaactga gataaagtcg
    19021 gttaatatac cagatagttt taaaacacta aaagttggca acacatatga tttaaatgtt
    19081 gtagtagagc catctaatca aagtaagtta ttgaaataca caacagatca aacgaatatt
    19141 gtatcaatca atagtgatgg tcaagtcact gcggaagcac aaggtattgc cacggttaaa
    19201 gcatatgttg gtaatatgag tgacactata acaataaatg tagaagcata agaggggaca
    19261 acccctctat tttatttgaa aataaggaga gtattataaa atggcaaaat taaaacgtaa
    19321 cattattcaa ttagtagaag atccaaaagc aaatgaaatt aaattacaaa cgtacttaac
    19381 accacacttc atttcatttg aaattgtata cgaagcaatg gatttaatcg atgatattga
    19441 ggacgaaaat agcacgatga agccaagaga aatcgctgac agattgatgg atatggttgt
    19501 aaaaatttac gataaccaat tcgcagttaa agacctaaaa gaacgtatgc atgcacctga
    19561 tggaatgaat gcacttcgtg aacaagtggt tttcattacg caaggtcaac aaactgagga
    19621 aactagaaat tttatccaga acatgaaata aagcctgaag atttaacata taaagcaatg
    19681 ttgaaaaata tggatactct catgatggac ttaattgaaa atggtaaaga cgctaacgaa
    19741 gttttaaaaa tgccatttca ttatgtgctt tccatatatc aaaataaaaa caatgacatt
    19801 tctgaagaaa aagcagaggc tttaattgat gcattttaac cttaaccgtt tggttagggt
    19861 tatttttttg aactttttta gaaaggaggt aaaaaatggg agaaagaata aaaggtttat
    19921 ctataggttt ggatttagat gcagcaaatt taaatagatc atttgcagaa atcaaacgaa
    19981 actttaaaac tttaaattct gacttaaaat taacaggcaa caacttcaaa tataccgaaa
    20041 aatcaactga tagttacaaa caaaggatta aagaacttga tggaactatc acaggttata
    20101 agaaaaacgt tgatgattta gctaagcaat atgacaaggt atctcaagaa cagggcgaaa
    20161 acagtgcagg agctcaaaag ttacgacaag aatataacaa acaagcaaat gaactgaatt
    20221 atttagaaag agaattacaa aaaacatcag ccgaatttga agagttcaaa aaagctcaag
    20281 ttgaagctca aagaatggca gaaagtggct ggggaaaaac cagtaaagtt tttgaaagta
    20341 tgggacctaa attaacaaaa atgggtgatg gtttaaaatc cattggtaaa ggcatgatga
    20401 ttggtgtaac tgcacctgtt ttaggtatcg cagcagcatc aggaaaagct tttgcagaag
    20461 ttgataaagg tttagatact gttactcaag caacaggcgc aacaggcagt gaattaaaaa
    20521 aattgcagaa ctcatttaaa gatgtttatg gcaattttcc agcagatgct gaaactgttg
    20581 gtggagtttt aggagaagtt aatacaagat taggttttac aggtaaagaa cttgaaaatg
    20641 ccacagagtc attcttgaaa ttcagtcata taacaggttc tgacggtgtg caagccgtac
    20701 agttaattac ccgtgcaatg ggcgatgcag gtatcgaagc aagtgaatat caaagtgttt
    20761 tggatatggt agcaaaagcg gcgcaagcta gtgggataag tgttgacaca ttagctgata
    20821 gtattactaa atacggcgct ccaatgagag ctatgggctt tgagatgaaa gaatcaattg
    20881 ctttattctc tcaatgggaa aagtcaggcg ttaatactga aatagcattc agtggtttga
    20941 aaaaagctat atcaaattgg ggtaaagctg gtaaaaaccc aagagaagaa tttaagaaga
    21001 cattagcaga aattgaaaag acgccggata tagctagcgc aactatttta gcgattgaag
    21061 catttggtgc aaaggcaggt cctgatttag cagacgctat taaaggtggt cgctttagtt
    21121 atcaagaatt tttaaaaact attgaagatt cccaaggcac agtaaaccaa acatttaaag
    21181 attctgaaag tggctccgaa agatttaaag tagcaatgaa taaattaaaa ttagtaggtg
    21241 ctgatgtatg ggcttctatt gaaagtgcgt ttgctccagt catggaagaa ttaataaaaa
    21301 agctatcagt cgctgtcgat tggttttcga atttaagtga tggttctaaa agatcaattg
    21361 ttattttcgg tggtattgct gctgcaattg gtcctgtagt ttttgggtta ggtgcattta
    21421 taagcacaat tggtaatgca gtaactgtat tagccccact attagctggt attgcaaagg
    21481 ctgatggatt aattagtttt ttatcgacta aagtacctat attaggaact gtcttcacag
    21541 ctttaactgg tccaattggc attgtattag gtgttttagc tggcctagca gtcgcattta
    21601 caattgctta taagaaatct gaaacattta gaaattttgt taatgatgca attgaaagtg
    21661 ttaaacaaac atttagtaat tttattcaat ttattcaacc tttcattgat tccgttaaaa
    21721 acgtctttaa acaagcggtt tcagcaatcg ttgatttcgc taaagatatt tggagtcaaa
    21781 ttaatggatt ctttaatgaa aacggaattt ctattgttca agcgcttcaa aatatatgca
    21841 attttatcaa agttatattt gaatttatct taaattttgt aattaaacca ataatgtttg
    21901 caatctggca agtgatgcaa tttatttggc cggcggttaa agccttgatt gtcagtactt
    21961 gggagaatat aaaaggtgtg atacaaggaa ctttaaatat catactaggt ttaattaagt
    22021 tcttctcaag tttatttact ggagattggc gaggagtttg ggacgcgatt attatgattc
    22081 ttaaaggagt cgttcaatta atatggaatt taattcaatt atggtttgta ggcaaaatac
    22141 ttggcgttgt taggtacttt ggcggattgc taaaaggatt aataacaggt atttgggacg
    22201 taataaaaag tatattcagt aaatctttat cagcaatttg gaatgcgaca aaaagtattt
    22261 ttggattctt atttaatagt gtcaaatcaa ttttcacgaa tatgaaaaat tggttatcta
    22321 acacatggag tagtatccgt acgaatacga taggaaaagc acagtcatta tttagtggtg
    22381 tgaaatcaaa attcactagt ttgtggaatg cgacaaaaga tatttttagt aatctaagaa
    22441 attggatggc aaatatttgg aattccatta aagataatac ggttggaatt gcaagtcgtt
    22501 tatggagtaa agtgcgtgga atttttacaa atatgcgtga cggcttacaa agtattatca
    22561 gcaaaattaa aagtcatatc ggcggtatgg tagatgctat taaaaaagga cttaataaat
    22621 taattgaagg tttaaactgg gtcggtggta agctgggaat ggataaaata cctaagttac
    22681 acactggtac agaacacaca catactacta caagattagt taagaacggt aagattgcgc
    22741 gggatacgtt cgctacggtt ggggataaag gacgtggaaa tggtccgaat ggtttcagaa
    22801 atgaaataat tgaattccct aatggtaaac gtgtaatcac acctaataca gatactaccg
    22861 cttatttacc taaaggctca aaagtataca acggtacaca aacttattca atgttaaacg
    22921 gaacgcttcc aagatttagc ttaggtacta tgtggaaaga tattaaatcc ggtgcatcat
    22981 cggcatttaa ctggacaaaa gatcaaatag gtaaaggtac caaatggctt ggcgataaag
    23041 ttggcgatgt tttagatttt atggaaaatc ctggcaaact tttaaattat atacttgaag
    23101 cttttggaat tgatttcaat tctttaacta aaggtatggg aattgcaggc gacataacaa
    23161 aagctgcatg gtctaagatt aagaaaagtg ctactgattg gataaaagaa aatttagaag
    23221 ctatgggcgg tggcgattta gtcggcggaa tattagaccc tgacaaaatt aattatcatt
    23281 atggacgtac cgcagcttat actgctgcaa ctggaagacc atttcatgaa ggtgtcgatt
    23341 ttccatttgt atatcaagaa gttagaacgc cgatgggtgg cagacttaca agaatgccat
    23401 ttatgtctgg tggttatggt aattatgtaa aaattactag tggagttatc gatatgctat
    23461 ttgcgcattt gaaaaacttt agcaaatcac cacctagtgg cacgatggta aagcccggcg
    23521 atgttgttgg tttaactggt aataccggat ttagtacagg accacactta cattttgaaa
    23581 tgagaagaaa tggacgtcac tttgaccctg aaccatattt aagaaatgca aagaaaaaag
    23641 gtaggttatc aattggtggc ggtggtgcta cttctggaag tggtgcaact tatgcaagcc
    23701 gagtaatccg tcaagcgcaa agtattttag gaggacgtta taaaggtaag tggattcatg
    23761 accagatgat gcgagttgca aagcgtgaaa gtaactatca atcaaatgca gtgaataatt
    23821 gggatattaa tgctcaaaga ggagacccat caagaggatt attccaaatc atcggctcaa
    23881 cttttagagc aaacgctaaa cgtggatata ctaactttaa taatccggtt catcaaggta
    23941 tttcagcaat gcagtacatt gttagacgct acggttggga tggatttaag cgtgctggtg
    24001 attacgcata tgctacaggt ggaaaagttt ttgatggttg gtataactta ggtgaagacg
    24061 gtcatccaga atggattatt ccaacagatc cagctcgtag aaatgatgca atgaagatgt
    24121 tgcattatgc agcagcagaa gtaagaggga gaaaagcgag taaaaataag cgtcctagtc
    24181 aattgtctag tgtaaatggg tttgatgacc caagcttatt attgaaaatg attgaacaac
    24241 agcaacaaca aatagcttta ttactgaaaa tagcgcaatc caacgatgtg attgcagata
    24301 aagattatca gccgattatt gacgaatacg cttttgataa aaaggtgaac gcgtctatag
    24361 aaaagcgaga aaggcaagaa tcaacaaaag taaagtttag aaaaggagga attgctattc
    24421 aatgatagac actattaaag tgaacaacaa aacaattcct tggttgtatg tcgaaagagg
    24481 gtttgaaata ccctctttta attatgtttt aaaaactgaa aatgtagatg gacgttcggg
    24541 gtctatatat aaagggcgta ggcttgaatc ttatagtttt gatatacctt tggtggtacg
    24601 taatgactat ttatctcaca acggctttaa aacacatgat gacgtcttga atgaattagt
    24661 aaagtttttt aactacgagg aacaagttaa attacaattc aaatctaaag attggtactg
    24721 gaacgcttat tttgaaggac caataaagct gcacaaagaa tttgcaatac ctgttaagtt
    24781 cactatcaaa gtagtactaa cagaccctta caaatattca gtaacaggat ataaaaatac
    24841 tgcgatttca gaccaagttt cagttgtaaa tagtgggact gctgacactc ctttaattgt
    24901 tgaagcccga gcaattaaac catctagtta ctttatgatc actaaaaatg atgaagatta
    24961 ttttatggtt ggtgatgatg aggtaaccaa agaagttaag gattacatgc ctcctgttta
    25021 tcatagtgag tttcgtgatt tcaaaggttg gactaagatg attactgaag atattccaag
    25081 taatgattta ggtggtaaag tcggcggtga ctttgtgata tccaatcttg gcgaaggata
    25141 taaagcaact aatttttctg atgcaaaagg ttgggttggt gctggcacga aacgagggct
    25201 ccctaaagcg atgacagatt ttcaaattac ctataaatgt attgttgaac aaaaaggtaa
    25261 aggtgccgga agaacagcac aacatattta tgatagtgat ggtaagttac ttgcttctat
    25321 tggttatgaa aataaatatc atgatagaaa aataggacat attgttgtta cgttgtataa
    25381 ccaaaaagga gaccccaaaa agatatacga ctatcagaat aaaccgataa tgtataactt
    25441 ggacagaatc gttgtttata tgcggctcag aagagtaggt aataaatttt ctattaaaac
    25501 ttggaaattt gatcacatta aagacccaga tagacgtaaa cctattgata tggatgagaa
    25561 agagtggata gatggcggta agttttatca gcgtccagct tctatcatag ctatctatag
    25621 tgcgaagtat aatggttata agtggatgga gatgaatgga ttaggttcat tcaatacgga
    25681 gattctacca aaaccgaaag gcgcaaggga tgtcattata caaaaaggtg atttagtaaa
    25741 aatagatatg caagcaaaaa gtgttgtcat caatgaggaa ccaatgttga gcgagaaatc
    25801 gtttggaagt aattatttca atgttgattc tgggtacagt gaattaatca tacaacctga
    25861 aaacgtcttt gatacgacgg ttaaatggca agatagatat ttatagaaag gagatgagag
    25921 tgtgatacat gttttagatt ttaacgacaa gattatagat ttcctttcta ctgatgaccc
    25981 ttccttagtt agagcgattc ataaacgtaa tgttaatgac aattcagaaa tgcttgaact
    26041 gctcatatca tcagaaagag ctgaaaagtt ccgtgaacga catcgtgtta ttataaggga
    26101 ttcaaacaaa caatggcgtg aatttattat taactgggtt caagatacga tggacggcta
    26161 cacagagata gaatgtatag cgtcttatct tgctgatata acaacagcta aaccgtatgc
    26221 accaggcaaa tttgagaaaa agacaacttc agaagcattg aaagatgtgt tgagcgatac
    26281 aggttgggaa gtttctgaac aaaccgaata cgatggctta cgtactacgt catggacttc
    26341 ttatcaaact agatatgaag ttttaaagca attatgtaca acctataaaa tggtattgga
    26401 tttttatata gagcttagtt ctaataccgt caaaggtaga tatgtggtac tcaaaaagaa
    26461 aaacagctta ttcaaaggta aagaaattga gtatggtaaa gatttggttg ggttaactag
    26521 gaagattgat atgtcagaaa tcaaaacagc attaattgct gtgggacccg aaaatgacaa
    26581 aggaaagcgt ttagagctag ttgtgacaga tgacgaagcg caaagtcaat tcaacctacc
    26641 tatgcgctat atttggggga tatatgaacc acaatcagat gatcaaaata tgaatgaaac
    26701 acgattaagt tctttagcca aaacagagtt aaataaacgt aagtcggcag ttatgtcata
    26761 tgagattact tctactgatt tggaagttac gtatccgcac gagattatat caattggcga
    26821 tacagtcaga gtaaaacata gagattttaa cccgccattg tatgtagagg cagaagttat
    26881 tgctgaagaa tataacataa tttcagaaaa tagcacatat acattcggtc aacctaaaga
    26941 gttcaaagaa tcagaattac gagaagagtt taacaagcga ttaaacctaa tacaccaaaa
    27001 attaaacgac aatattagca atatcaatac tatagtaaaa gatgttgtag atggtgaatt
    27061 agaatacttt gaacgcaaaa ttcataaaag tgatacaccg ccagaaaatc cagtcaatga
    27121 tacgctttgg tatgatacaa gtaaccctga tgttgctgtc ttgcgtagat attggaatgg
    27181 tcgatggatt gaagcaacac caaatgatgt tgaaaaatta ggtggtataa caagagagaa
    27241 agcgctattc agtgaattaa acaatatttt tattaattta tctatacaac acgctagtct
    27301 tttgtcagaa gctacagaat tactgaatag cgagtactta gtagataatg atttgaaagc
    27361 ggacttacaa gcaagtttag acgctgtgat tgatgtttat aatcaaatta aaaataattt
    27421 agaatctatg acacccgaaa ctgcaacgat tggtcggttg gtagatacaa aaactttatt
    27481 tcttgagtat agaaagaaat tacaagatgt ttatacagat gtagaagatg tcaaaatcgc
    27541 catttcagat agatttaaat tattacagtc acaatacact gatgaaaaat ataaagaagc
    27601 gttggaaata atagcaacaa aatttggttt aacggtgaat gaagatttgc agttagtcgg
    27661 agaacctaat gttgttaaat cagctattga agcagctaga gaatccacaa aagaacaatt
    27721 acgtgactat gtaaaaacat cggactataa aacagacaaa gacggtattg ttgaacgttt
    27781 agatactgct gaagctgaga gaacgacttt aaaaggtgaa atcaaagata aagttacgtt
    27841 aaacgaatat cgaaacggat tggaagaaca aaaacaatat actgatgacc agttaagtga
    27901 tttgtccaat aatcctgaga ttaaagcaag tattgaacaa gcaaatcaag aagcgcaaga
    27961 agctttaaaa tcatacattg atgctcaaga taatcttaaa gagaaggaat cgcaagcgta
    28021 tgctgatggt aaaatttcgg aagaagagca acgcgctata caagatgctc aagctaaact
    28081 tgaagaggca aaacaaaacg cagaactaaa ggctagaaac gctgaaaaga aagctaatgc
    28141 ttatacagac aacaaggtca aagaaagcac agatgcacag aggagaacac tgactcgcta
    28201 tggttctcaa attatacaaa atggtaagga aatcaaatta agaactacta aagaagagtt
    28261 taatgcaacc aatcgtacac tttcaaatat attaaacgag attgtccaaa acgttacaga
    28321 tggaacaaca atcagatatg atgataacgg agtggctcaa gctttaaatg tggggccacg
    28381 tggtattaga ttaaatgctg ataaaattga tattaacggt aatagagaaa taaaccttct
    28441 tatccaaaat atgcgagata aagtagataa aaccgatatt gtcaacagcc ttaatttatc
    28501 aagagagggt cttgatatca atgttaatag aattggaatt aaaggcggta acaataacag
    28561 atatgttcaa atacagaatg attctattga actaggtggt attgtgcaac gaacttggaa
    28621 aggcaaacga tcaaccgatg atatattcac acgtcttaaa gatggacatc taaggtttag
    28681 aaataatacc gcaggcggtt cactttatat gtcacatttt ggtatttcaa catatattga
    28741 tggagaaggc gaagacggag gttcatccgg tactattcaa tggtgggata aaacttacag
    28801 tgatagcggt atgaatggca taacaatcaa ttcctatggt ggtgtcgttg cactaacgtc
    28861 agataataat cgggttgttc tggagtctta cgcttcatcg aatatcaaaa gcaaacaggc
    28921 accggtgtat ttatatccaa acacagacaa agtgcctgga ttaaaccgat ttgcattcac
    28981 gctgtctaat gcagataatg cttattcgag tgacggttat attatgtttg gttctgatga
    29041 gaactatgat tacggtgcgg gtatcaggtt ttctaaagaa agaaataaag gtcttgttca
    29101 aattgttaat ggacgatatg caacaggtgg agatacaaca atcgaagcag ggtatggcaa
    29161 atttaatatg ctgaaacgac gtgatggtaa taggtatatt catatacaga gtacagacct
    29221 actgtctgta ggttcagatg atgcaggaga taggatagct tctaactcaa tttatagacg
    29281 tacttattcg gccgcagcta atttgcatat tacttctgct ggcacaattg ggcgttcgac
    29341 atcagcgcgt aaatacaagt tatctatcga aaatcaatat aacgatagag atgaacaact
    29401 ggaacattca aaagctattc ttaacttacc tattagaacg tggtttgata aagctgagtc
    29461 tgaaatttta gctagagagc tgagagaaga tagaaaatta tcggaagaca cctataaact
    29521 tgatagatac gtaggtttga ttgctgaaga ggtggagaat ttaggattaa aagagtttgt
    29581 cacgtatgat gacaaaggag aaattgaagg tatagcgtat gatcgtctat ggattcatct
    29641 tatccctgtt atcaaagaac aacaactaag aatcaagaaa ttggaggagt caaagaatgc
    29701 aggataacaa acaaggatta caagctaatc ctgaatatac aattcattat ttatcacagg
    29761 aaattatgag gttaacacaa gaaaacgcga tgttaaaagc gtatatacaa gaaaataaag
    29821 aaaatcaaca atgtgctgag gaagagtaat ctttagcact atttttatac aaaaatttaa
    29881 ggaggtcatt taattatggc aaaagaaatt atcaacaata cagaaaggtt tattttagta
    29941 caaatcgaca aagaaggtac agaacgtgta gtatatcaag atttcacagg aagttttaca
    30001 acttctgaaa tggttaacca tgctcaagat tttaaatctg aagaaaacgc taagaaaatt
    30061 gcggagacgt taaatttgtt atatcaatta actaacaaaa aacaacgtgt gaaagtagtt
    30121 aaagaagtag ttgaaagatc agatttatct ccagaggtaa cagttaacac tgaaacagta
    30181 tgaaaagcta tgagttagat actcataatc tttattcttt tagaaagcgg gtgtactgaa
    30241 ttggggtggt tcaaaaaaca cgaacatgaa tggcgcatca gaaggttaga agagaatgat
    30301 aaaacaatgc tcagcacact caatgaaatt aaattaggtc aaaaaaccca agagcaagtt
    30361 aacattaaat tagataaaac cttagatgct attcaaaaag aaagagaaat agatgaaaag
    30421 aataagaaag aaaatgataa gaacatacgt gatatgaaaa tgtgggtgct tggtttagtt
    30481 gggacaatat ttggatcgct aattatagca ttattgcgta tgcttatggg catataagag
    30541 aggtgaataa aatgtttaaa ctaatctttg gttatagttt ctggacatgt ttttggttcg
    30601 gtaaatgtaa ataagtttta gtcagtgctt cggtactgac tttttattta ttgttgtaat
    30661 tatggtaata tgcagaagtg agcaagttgg atagatggtg gctatctgag tataaggagg
    30721 tggtgcctat ggtggcatta ctgaaatctt tagaaaggag acgcctaatg attacaatta
    30781 gtaccatgtt gcagtttggt ttattcctta ttgcattgat aggtctagta atcaagctta
    30841 ttgaattaag caataaaaaa taaccatcgc taactttggc tggtttcgat ggttaaatgg
    30901 ttattaattt aatctttaat ctaaaatagc caccgtcttt ttaacgggct cattagggta
    30961 acatgtttgc gcatgttgcc ctttttctat atataaatta acacaccata atataaatat
    31021 caaatagacg gcttattagt cgtcttttta ttttgggtaa aaggagataa gaatatgatt
    31081 aattggaaaa ttagaatgaa acaaaaatca ttttgggtag cgatattgtc agctatcttt
    31141 ttatttgctc aaaacatcgc aaaagctatt gggtatgata tccaagttta tacagagcaa
    31201 ttaacagacg gtttaaacgc tatattagga tttttagtat taactggtgt gattcaagac
    31261 ccgactacta aaggtatagg tgatagccac caagctttag aatatgaaga accaagaaga
    31321 aaatactagg aggtaaaata atgaaaacat acagtgaagc aagagcaagg ttacgttggt
    31381 atcaaggtag atatattgat tttgacggtt ggtatggtta ccaatgtgca gatttagcag
    31441 ttgattacat ttattggttg ttagaaatta gaatgtgggg aaatgcaaaa gatgcaatca
    31501 ataacgattt taaaaacatg gcaacagtat atgaaaacac accatcgttt gttccacaaa
    31561 taggtgatgt ggctgtattt accaaaggaa tatataaaca atacggtcat attggtttag
    31621 tgtttaatgg tggtaataca aaccaatttt taattttgga acagaactat gacggtaacg
    31681 caaatacgcc tgcaaagtta cgttgggata attattacgg ctgtactcac tttattagac
    31741 ctaagtataa aagtgagggc ttaatgaata agatcacaaa taaagttaaa ccacctgctc
    31801 aaaaagcagt cggtaaatct gcaagtaaaa taacagttgg aagtaaagcg ccttataacc
    31861 ttaaatggtc aaaaggtgct tattttaatg cgaaaatcga cggcttaggt gctacttcag
    31921 ccactagata cggtgataat cgtactaact atagattcga tgttggacag gctgtatacg
    31981 cgcctggaac attaatatat gtgtttgaaa ttatagatgg ttggtgtcgc atttattgga
    32041 acaatcataa tgagtggata tggcatgaga gattgattgt gaaagaagtg ttttaattct
    32101 taggttaaaa tgttaaatat ttgttaatta ttttttaatg taattttagt ttcttttaat
    32161 attttattga tttttaatat tttctcaata taaaatgaag ttgttgatat ttatcatctt
    32221 aaataagggt gttagctata aaaagagata aataaaaaca aatatattat atttggagga
    32281 agcgccatgc tcaaaagagg tttattattt ttaactgttt tattgttatt attctcattt
    32341 tcttcaatta ctaatgaggt aagtgcatca agttcattcg acaaaggaaa atataaaaaa
    32401 ggcgatgacg cgagttattt tgaaccaaca ggcccgtatt tgatggtaaa tgtgactgga
    32461 gttgatggta aaggaaatga attgctatcc cctcattatg tcgagtttcc tattaaacct
    32521 gggactacac ttacaaaaga aaaaattgaa tactatgtcg aatgggcatt agatgcgaca
    32581 gcatataaag agtttagagt agttgaatta gatccaagcg caaagatcga agtcacttat
    32641 tatgataaga ataagaaaaa agaagaaacg aagtctttcc ctataacaga aaaaggtttt
    32701 gttgtcccag atttatcaga gcatattaaa aaccctggat tcaacttaat tacaaaggtt
    32761 attatagaaa agaaataaaa caaaatagtt gtttattata gaaagcaatg tcttgattga
    32821 atatgtgtag tgaaaattat ctttcatcaa attctcattc atgcacgaat ggttcttccc
    32881 cacctaatca gatattaggt gacttatggg gagaaatcag ttaggatgaa aaagtggata
    32941 atcctttttt aggcaggtac ttcggtactt gcctattttt ttatgttata atctttctag
    33001 acgtattcaa gggacgtctt tttagattgt atgttatagc tagctttcgg gctagttttt
    33061 tgttatgatg tgttacacat gcatcaacta tttacatcta tccttgttca cccaagcatg
    33121 tcactgggtg ttttttctta tgatagagag catagttttc atactactcc ctcgtagtat
    33181 atatgacttt agcattcccg tataatagtt tacggggtgc tttttatgtt ataattaact
    33241 gtatatagta ggagtgaact atatagcctg ttaagtggcc tagtaaccta acacttatcc
    33301 tgcaattgat atcctttttg cccttcactc gatacatata tctcaacaac atagaaatat
    33361 tacagtcgct acaccgcatc ttaaatggtg tggttatttt tattggaagt gtgtatcagg
    33421 tatcagtaat gttaaaacac cagctaaaaa tgaaaagaat tcaccagtgc cagcaggtta
    33481 tacactcgat aaaaacaatg taccgtataa aaaagagact ggttattaca cagttgccaa
    33541 tgttaaaggt aataacgtga gggatggcta ttcaactaat tcaagaatta caggtgtatt
    33601 acccaataac gcaactatca aatatgacgg cgcatattgc attaatggct atagatggat
    33661 tacttatatt gctaatagtg gacaacgtcg ttatatagcg acaggagagg tagacaaggc
    33721 aggtaataga ataagcagtt ttggtaagtt tagtgcagtt tgataattag atatataaag
    33781 gtttggcaag ttatgaaatg tctgccaaac ctttatataa aaaagaaata tctacctttt
    33841 aatttatgta actactatta gtatgcatat tcattagttt ttccaggacc attaattaca
    33901 taagatgatt tagactctcc ttttttaaag aagtatgttt tatacatttt acctagtaac
    33961 tcaacatttt ttctatcttc agcaagtggt gtattcagat atactgtata gtaaccttta
    34021 ttttcagtta aaataaccat tttttcaaat tgagcagaat tttttgtgcc tttctttaaa
    34081 taatttctca aacgttcatc taattttcct agcgttgtag gaagaccact atttttaaat
    34141 gattctttat aagctttttc tttctctaac attttcttat ttgattctat ttcttcattt
    34201 gtaggaaacg gttcaaaagt aaaagctttc gctgaatgat ggtgtgtact gattcctgcc
    34261 gttaaaaaac ttaatgctaa aactgttgtt gctaatttct ttttcataat gatgttaatt
    34321 ctccttaaat tctatattta aatttatagt tagttttgcg aaattcctaa aatgagttta
    34381 atctaatcga cgaaatatat ta
//
LOCUS       DANJFZ010000019        32695 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.019, whole genome shotgun sequence.
ACCESSION   DANJFZ010000019 DANJFZ010000000
VERSION     DANJFZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.019"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(176..1042)
                     /locus_tag="PWN45_000780"
     CDS             complement(176..1042)
                     /locus_tag="PWN45_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL0622564.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            1260..2324
                     /locus_tag="PWN45_000781"
     CDS             1260..2324
                     /locus_tag="PWN45_000781"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL0622565.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2479..2754
                     /locus_tag="PWN45_000782"
     CDS             2479..2754
                     /locus_tag="PWN45_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622566.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2940..3662)
                     /locus_tag="PWN45_000783"
     CDS             complement(2940..3662)
                     /locus_tag="PWN45_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL0622567.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4016..4465
                     /locus_tag="PWN45_000784"
     CDS             4016..4465
                     /locus_tag="PWN45_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDL0622568.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4546..5133)
                     /locus_tag="PWN45_000785"
     CDS             complement(4546..5133)
                     /locus_tag="PWN45_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0622569.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(5111..5209)
                     /locus_tag="PWN45_000786"
     CDS             complement(5111..5209)
                     /locus_tag="PWN45_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622570.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            5266..6948
                     /locus_tag="PWN45_000787"
     CDS             5266..6948
                     /locus_tag="PWN45_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDL0622571.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7077..7460)
                     /locus_tag="PWN45_000788"
     CDS             complement(7077..7460)
                     /locus_tag="PWN45_000788"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDL0622572.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(7462..8313)
                     /locus_tag="PWN45_000789"
     CDS             complement(7462..8313)
                     /locus_tag="PWN45_000789"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDL0622573.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(8306..9124)
                     /gene="panB"
                     /locus_tag="PWN45_000790"
     CDS             complement(8306..9124)
                     /gene="panB"
                     /locus_tag="PWN45_000790"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDL0622574.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            9197..10057
                     /locus_tag="PWN45_000791"
     CDS             9197..10057
                     /locus_tag="PWN45_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0622575.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(10143..10847)
                     /gene="budA"
                     /locus_tag="PWN45_000792"
     CDS             complement(10143..10847)
                     /gene="budA"
                     /locus_tag="PWN45_000792"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL0622576.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(11125..12084)
                     /locus_tag="PWN45_000793"
     CDS             complement(11125..12084)
                     /locus_tag="PWN45_000793"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0622577.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            12771..14219
                     /locus_tag="PWN45_000794"
     CDS             12771..14219
                     /locus_tag="PWN45_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0622578.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(14300..15637)
                     /locus_tag="PWN45_000795"
                     /pseudo
     CDS             complement(14300..15637)
                     /locus_tag="PWN45_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            15895..16311
                     /locus_tag="PWN45_000796"
     CDS             15895..16311
                     /locus_tag="PWN45_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622579.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            16434..17324
                     /locus_tag="PWN45_000797"
     CDS             16434..17324
                     /locus_tag="PWN45_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDL0622580.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(17517..19013)
                     /gene="mqo"
                     /locus_tag="PWN45_000798"
     CDS             complement(17517..19013)
                     /gene="mqo"
                     /locus_tag="PWN45_000798"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDL0622581.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            19309..19638
                     /locus_tag="PWN45_000799"
     CDS             19309..19638
                     /locus_tag="PWN45_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622582.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(19698..21296)
                     /locus_tag="PWN45_000800"
     CDS             complement(19698..21296)
                     /locus_tag="PWN45_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDL0622583.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(21490..21933)
                     /locus_tag="PWN45_000801"
     CDS             complement(21490..21933)
                     /locus_tag="PWN45_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL0622584.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(22214..22426)
                     /locus_tag="PWN45_000802"
     CDS             complement(22214..22426)
                     /locus_tag="PWN45_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDL0622585.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(22704..24413)
                     /gene="betA"
                     /locus_tag="PWN45_000803"
     CDS             complement(22704..24413)
                     /gene="betA"
                     /locus_tag="PWN45_000803"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDL0622586.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(24674..26164)
                     /gene="betB"
                     /locus_tag="PWN45_000804"
     CDS             complement(24674..26164)
                     /gene="betB"
                     /locus_tag="PWN45_000804"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDL0622587.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            26233..26328
                     /locus_tag="PWN45_000805"
     CDS             26233..26328
                     /locus_tag="PWN45_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622588.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            26417..26980
                     /locus_tag="PWN45_000806"
     CDS             26417..26980
                     /locus_tag="PWN45_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDL0622589.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(27168..27854)
                     /locus_tag="PWN45_000807"
     CDS             complement(27168..27854)
                     /locus_tag="PWN45_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0622590.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(28118..29740)
                     /locus_tag="PWN45_000808"
     CDS             complement(28118..29740)
                     /locus_tag="PWN45_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL0622591.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            29872..30033
                     /locus_tag="PWN45_000809"
     CDS             29872..30033
                     /locus_tag="PWN45_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622592.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(30049..30159)
                     /locus_tag="PWN45_000810"
     CDS             complement(30049..30159)
                     /locus_tag="PWN45_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622593.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(30259..30795)
                     /gene="nrdG"
                     /locus_tag="PWN45_000811"
     CDS             complement(30259..30795)
                     /gene="nrdG"
                     /locus_tag="PWN45_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL0622594.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(30792..32642)
                     /gene="nrdD"
                     /locus_tag="PWN45_000812"
     CDS             complement(30792..32642)
                     /gene="nrdD"
                     /locus_tag="PWN45_000812"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL0622595.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
BASE COUNT        10338 a         6165 c         5027 g        11165 t
ORIGIN      
        1 aatcactttt attgtaactc aaccttgttt tacattacag aaattaacaa tagcgattaa
       61 aattttattt tccaacaata aaaacgcaca aatattttta ttgaggcagc tgtatcgctt
      121 ctcattgtaa aaatatctgt gcatattatc tatttatctc aatctaacgt cttaactaac
      181 ttgttgtatc ttgtaaaatc ttatccatag aatgcgcaac actatcacgg tacatctcac
      241 caaatttcaa taaatggacc atcaataaat ataaacgata aaattcaagt ctataggatg
      301 caccttttgc gagtggataa tgtttattat acgcatcata aaattcgctc gtaaaaccac
      361 caaataccgt tgtaataccg atatcgaatt ctctgtcacc atataatggc gctggatcaa
      421 ataacgccgg acgaccatct tgtaagaaca tataattacc accccatagg tcaccatgta
      481 ataaagacgg tttactttga tgcttttcta attccgccac aatttgacgt cgcactttgt
      541 catatacttt gatatcgttg gcatcccata gccctctatt tagcaactca tctttcaaat
      601 ggtccaagcg tttgtcgaca aaaatggtac accaatcgtc ttgccaatga ttatcaaaag
      661 aaatatcgcc accttcataa ggtaatgaga agccaaattt gccttcttct tgttgctgac
      721 tgtgtaattg agctacgagt tgccctaatt ggcgttgact ccctgaagcc ccttcttcta
      781 aatacgtcat cactaaatac gcatcaccgt taacctcgcc acttgcaatt actctaggtg
      841 ccgtgatacc tgcacgttca aattcattta aacctgcaat ttctgcagca taaaatgatt
      901 ctttacgtcc acgttggaca agtaagaaaa atgtatccgt atctgtttcg actcgatatg
      961 cttcgtttac atcaccacca ctcactggtg aaatctcttt aatatctttt aaaggtaaat
     1021 gctctaacca ttgttcattc atacaaacac cttccaatct acttacttta tactataata
     1081 ttgcctaatt aggatgattt aatcttaaaa acgttattca aacttgcgtc aattttcaaa
     1141 actttttagt tttaactagg aaattatgta tggaaatgct agtctattaa aagaatatat
     1201 ttcacatata ttaagttgag gatagtaagc ctcaaatata atgaaatagg agcatactca
     1261 tgtacaaatt aattaaacct ttcttattca aaatcgaacc cgaaaaagca cacggactaa
     1321 ctatcgatgc attaaaaacg ttacaaaagt ttccggtttt attcccagtc gtcgataaac
     1381 tatttactta taagaatcca acgttatcac aaacgataca aggtaatacg tacgacaatc
     1441 caattggctt agcagctggt ttcgacaaat cttgcgaagt accaaaagca ttggaacacc
     1501 ttggattcgg tgctttagaa ttaggtggta tcacacctaa acctcaaccg ggtaaccctc
     1561 aaccacgcat gtttagatta ttagaagatg acgccttgat aaatcgaatg ggcttcaata
     1621 atattggtat gaacaaagca ctaagtcatt tgcgtaaaaa tgcttatcaa gtacctgttg
     1681 gtatcaatgt tggtgtgaat aaaatgacac cttatgaagc gcgttatcaa gattatataa
     1741 aggttattga tacgtttaaa cacgacgttt catttttcac agtcaacatc agttctccaa
     1801 atactgaaaa tcttcaaaac ttccatgata aagatgaatt ttcaatgtta tgccaagctt
     1861 taacagcatt taaaaaacaa catgatgtaa cagtgccaat ttatttaaaa ctaacgtctg
     1921 atatggattt cgatggctta aaagcactat taccagcgat tactgagaca tttgacggta
     1981 tcatcttagc aaacacaacg cgacaacgag atggtttaac ttctgctaat aaagtcgaag
     2041 aaggcggttt gagtggtcgt ccattatttg aacgtaattt aaaattgatt aagtatgctt
     2101 atcagcaaac aaatggtgaa tttttaatta taggtacagg cggcgtattc agtactgaag
     2161 atgcaatcaa aatgatgcgt cacggtgcgt cacttattca aatttattca tcacttgtta
     2221 ttgaaggccc aggtttaact aagaaaatga acaaaggcat cgcacgttac ttaaaagatc
     2281 atcattttga caatgtcagt gatattatag gactagatgc ctaatccaac atagtcaaat
     2341 aatactttaa aacttgctga atcgtcttat taaaattatg atataactca agaagatata
     2401 tttatcacca tttttcaaaa taatggctta aatatcacat atcattaaat atagcgtttt
     2461 ataacggagg cgtttgttat gactaaagaa acagataata aaaaaacagt agcgacttct
     2521 gacaacaaag acacacagca acaaaatcaa cctgaagaag atgaacgtca aggtttgaat
     2581 ggttatcgta aaacagatct tgatttagaa atcgagcaag agctacgtga aatgatgaaa
     2641 acaggagaaa atgaaacgaa aaatgactat aaaaagttta aagtgttttc acttatttca
     2701 acacttgtca ttgtcatttt agcaattata agatttgttc ataaaatgat gtaattagag
     2761 tgagacattg ttttatgtct caggatccag ttattcatta tatctacaat atttacgatt
     2821 atataaataa cccgagattt tagtatgatt catttcacta aaatctcggg tttctatttg
     2881 ataattttta atgggatatg gcatgtatac gttcttgcct tttatctcat ttccaatgat
     2941 taatctggat attgttctaa aaatgctttc gcttctttat taactgtttt aaaatcaata
     3001 ccttgttgca tcgctgcaaa gacacatcca caataacact gcctaaagat attatagtca
     3061 ttacacattt ctatggaacg ctcataacct ttacttttct taaaatcact tggcaaatag
     3121 ttcacatcgt atattttttg gacatccata ccaagttcat tgattaattg tgcgttcttt
     3181 ttaggtgata atgtgattgc actgccaaaa taatcatagc catgttctac agctgctttc
     3241 gctacaatat ccaaacgcat ttcaaaacaa gcggtacaac gtaagccgcc ttctttttca
     3301 tcagctaatt ctttatcctt caccattttc acaaacttat gcggttcata aggtgcttca
     3361 atatacttca cattcgcacc agtcttgcga ttaaaatctt ccacaaattg ttcttgcact
     3421 ttagcacgtc gtaagtactc atttttcgga tgaatatttg aattcgcgaa ataaattgca
     3481 atgtctgcat attgtgttaa aaactctaat gtatatgtac tacaaggtgc acaacaacta
     3541 tgcaataaga ttttaggtct gattgcttct ctttcccact gcccgattaa tttcttcaac
     3601 accttgtcat aattaatttt ttgatttttc attttactga taataggttc agcattaatc
     3661 atgatttaac ctcccacatt taatcattaa cttctattat atatgattca tattaaatgt
     3721 cagtcaaaaa agttagaaat tcattttaat gcatttatat ttcgaaaatc ccttatgtat
     3781 ctaaaagcat tttttaaact tgaattttaa aacactaaac aacacatacg tctctgtgtc
     3841 attttcattt tttgtatgtc atatatatgt ttacttcatt taaatcaatt tcatcttata
     3901 atttatcgtg tattttacaa aagattgact tcaattcatc gtaaaagtta tacttttgcc
     3961 attttttaat gtaacatggt gttagtaata aaaataatac attgaggtgt tttacatgac
     4021 agcattattc ccttatatcg cttttgaaaa ttcaaaagaa gcccttgcat attacgaaga
     4081 agtatttggt gcaactgacg ttaaacgttt agaagttggc gaagaacaag cgtcacattt
     4141 tggtatgact aaggaagaag cgcaagaagc aactatgcat gctgaatttg aagtgcttgg
     4201 cgtaaaagtg ttatgttctg attcttttgg tcgcgctgac aaaattaata atggcatatc
     4261 attattaatt gattatgatg ttaacaataa ggaagatgct gataaagttg aagcattcta
     4321 tgagcaaatt aaagatcatt cttcaattga aatagaatta ccgtttgctg accaattctg
     4381 gggtggcaaa atgggcgtct ttaccgataa atacggtgtt cgttggatgt tacatggtca
     4441 agactatact gcaatccaac aataatgtta ttgataaaga tatcatttaa taagcttatg
     4501 ttatttagca aggcaagcca ctctcgattc atggcttgcc ttattttatt gtatattttt
     4561 aattatcgtt gctggccctt gattgacaat cgtattaaaa tgccttatta agtcaacttt
     4621 gtctatacgg tttggatctt ctacccaatg tcttataaaa gacaatcccg cacctgaaac
     4681 ataactcatg aaataagaaa atggtatacc attaatttga tcattttcat ttttataacg
     4741 ttgtaaatga cacataatta aatcataaag tttttcgtgg atatttgaat ctttacccat
     4801 acgaaacatc aattgataaa atgcgatgtc tttttctatc atttctatta aaacggtcat
     4861 aatttgatgt atgttatccg tggataactt aactgctcca tttaacttct catcatgaat
     4921 gaagtctctt atttcctcca actgctggtc ctctaatttt tcaagcaaat catacttatc
     4981 ataataatgc gtataaaatg tactacggtt aacatcagct aaatctgcaa tttgttgcac
     5041 agtaatctct tctaattggt gttgatgtaa aagttcaata aatgcatttc tcattgcaac
     5101 ttgtgatttt ctaatacgtc gatctatagt catttatatc aagtcctccc caatgattat
     5161 aaacgttatg ttcattatcc cacaaatctc caacattgat gattggcaca caatgtttac
     5221 ctgtttaata taggtgatac aaacaaacag aaaaaggtga taacaatgaa ccaacattta
     5281 ctaggaaatc caaaattaac tgtaactcat gtcaatgaag ttaaagccgg tattaaccac
     5341 atcgttgtcg acagtgttca atatggaaat caagaaatga ttatggaaaa agatgtcact
     5401 gtggaaatgc gcgatggcga aaaattatat attaatattt tcagaccaaa taaagatggc
     5461 aaattccctg tagttatgtc tgcagatact tacggtaaag ataataagcc taaaatcaca
     5521 aatatgggtg ccctttggcc aacattaggt accattccga catctagttt tacacctgaa
     5581 gaatcaccag acccaggatt ttgggtgcca aatgattatg ttgtagttaa agttgcatta
     5641 cgcggtagtg acaaatccaa aggcgtctta tctccatggt caaaaagaga agcggaagat
     5701 tattacgaag tgattgaatg ggcagcaaat cagtcatgga gtaatggaaa tatcgggaca
     5761 aatggtgttt cttatcttgc ggtgactcaa tggtgggtcg catcattaaa tccaccacat
     5821 ttaaaagcaa tgattccttg ggaaggctta aatgatatgt atagagaagt agcctttcac
     5881 ggaggtatac cagatactgg cttttatcgt ttctggactc aaggtatttt tgcgagatgg
     5941 acagataatc caaatatcga agatttgatt caagcacaac aagaacatcc tctgttcgat
     6001 gatttttgga aacagcgtca agtgccatta tcacaaatta aaacacctct actaacatgt
     6061 gctagttggt ctacacaagg tttgcacaac cgtggctctt ttgaaggatt taaacaagct
     6121 gcatctgaag aaaaatggct atatgtgcat ggacgtaaag agtgggaaag ttactacgct
     6181 agagaaaatc tcgaacgcca aaaatcattc tttgattttt accttaaaga agaaaataac
     6241 gattggaaag atacgcctca tgtcatttat gaagttagag atcaatttta taaaggcgaa
     6301 ttcaaatcag cgtcagcgtt ccctttacct aacgcagaat atacaccatt gtatttgaat
     6361 gctgaaaatc acacattgaa tcatgcaaag attagtagcg cgcatgtcgc acaatatgac
     6421 tctgaagata aacaacaaga tgtaagtttt aaatatacgt ttgacaaaga tactgagtta
     6481 gttggaaaca tgaacttaaa actatgggta agcactaaag actcagatga tatggattta
     6541 tttgcaggta ttaaaaagtt agatcgtcgt ggtaatgaag ttaacttccc tgattttaat
     6601 catattgaaa atggtcaagt agctactggt tggttacgcg tatcacatcg tgaattagat
     6661 caagaaaaat cctcaatcgc gcaaccttgg cataaacatg aaacagaatt aaagttgtca
     6721 caagatgaga ttgtacctgt tgaaatcgaa ttgttacctt caggcacgct atttaaacaa
     6781 ggcgaaacat tggaagttgt tgtaaagggt agtgaaattg taattggtaa tagtactcct
     6841 ggcatgaaaa cacgttatga acatgaagaa accgtaaata aaggcatgca catgatttat
     6901 actggtggta aatatgattc acaattaatc attcctatcg ttaattgata tgcagcaatt
     6961 acggtcgctt ttgattaaaa gtgacatagt gataggactg tataacaaga gaaagccaca
     7021 cgcttggaat cttaaaccaa ggtgtggccc tttttattat tgatggctat ttaattttat
     7081 aacactatcg tattttcttt ttcatgaatc atttcaataa tgacattatc ttcattcatt
     7141 actgctactt taggtgcatg gtttttaatt tcttcttcat tcaactgtgc ataagtcatg
     7201 attatgacta catcgcctac ttcaacaagt cttgacgctg caccgtttaa acaaatttta
     7261 ccactacctc tttcaccagc tattacgtat gtttcaaaac gtgcaccatt attattattc
     7321 acgatggcta ctttttcatt tggcaagatg tctaccgctt ccaatatatc tgaatcaatc
     7381 gtaatgctac ctacataatt taaatttgac tcagtcactc ttgctctatg aattttagca
     7441 ttcatcattg ttcttatcac tttattcagc tccaattatt atattatcta ttaaacgcgc
     7501 ttttgaaaat ttaacagcta acgagataaa tatgcgtcca gttatttcgt gttgttctac
     7561 taattgagga taactataaa cagcaacttc ttcaatgcgt ccacttatat gtgattcaag
     7621 atattcagta accctgtcta taattacttt actttgacgt tcaccgtctt gatacaacgc
     7681 ttgtgctaat agcaaacttt tacttaaatg taccgcttct tgtcgttctt gctccgttaa
     7741 ataaacattt cttgaacttt tcgccaaacc atctgcttct cgaacgatat caataccaat
     7801 aatttcaacg gcatgattga agtcttttac catttgctcg acaatagcca attgctgggc
     7861 atctttttta ccaaaataag cataatccgg cataacaata ttaaatagct tattaactac
     7921 tgttaccacc ccatcaaaat gccctggacg cttcgctcct tctaacacat cagctaatgg
     7981 gcctactttg acatcaatac ctaattcacc tggatacata tcttctactg caggatgaaa
     8041 aacaatgtcc gctcctactt ctgatactaa ttctaaatct ttatcaattt gtctcggata
     8101 agcatcgaaa tcttcgtttg gaccaaattg taatggatta acaaatacac tcacaattgt
     8161 aatatcattt gtactaactg attcgcgtac catcgttaaa tgtccatcat gtaaggcacc
     8221 cattgttggg ataaaaccaa tcgttgtgcc tgagcgtttg gctgctttaa caatgtgttg
     8281 catctctttt accgtagtaa tcagcttagt cattgttatt aacctcattc ataattttct
     8341 ttttatacgt atattcttct gatggaaatg caccagattt aacttcttga tcgtattgtt
     8401 ttaaaccatc cacaccaaca ctaaaatcag caaattgctt cacaaatttc gctttatgtt
     8461 caacaccata atttaacata tcgtgataaa ccaatacttg accatctgta ccttttcctg
     8521 caccaatacc aatgactgga attgttaagt gcttgctaat ttcttctgct aaatcatttg
     8581 gaattgcttc aagtactaac gcaactgcac cagcttgttc tacatttttc gcgtctaaaa
     8641 taagttgctc cgctgcttct ttcgttgcac cttgtaattt ataccccata acgccaacac
     8701 tttgaggtgt taatcctaaa tgtgcaacaa caggaatacc aattgccgtt gctttttcaa
     8761 taaatggtgt aatatgcgct ccttctgctt taattgcatt tgcattcgtc tcctgataaa
     8821 gctttagagc atgatttaag tcttgtgtca tagagatgcc tactgcacca atcggcatat
     8881 caacaactac aaatgtattt ggtgcgcctc ttcttactgc acgaccgtga tgaatcatat
     8941 ctgctaacgt cacttgtacg gtactttcat aacctaatac agtcatacca agtgaatccc
     9001 caacaagaat catatcaata cccgctgctt ccacttgttt agcacttgga aaatcataag
     9061 ctgttaccat agaaatttta gtttgctttt gtttcatatc tattaattga cttactgttt
     9121 tcaatgttat tcaacctctt tttgcagtat tattagaaat agtataatga atcttttata
     9181 gaaaggaaag aagaagatgt tatcagttgc gattatcggc ccaggcgctg tcggtacaac
     9241 tattgcctat gaattacaac aatcattgcc tcatacaacg cttatcggga gacacgccaa
     9301 aacaataaca tattatactg taccacatgc acctgcacaa gatattgttg tgaaaggtta
     9361 tgaagatgtc acaaacacat ttgatgttat tattattgca gtcaaaacac atcaacttga
     9421 tgcagttatt cctcatttaa ctcatcttgc acatgaggac acgctcatca ttttagccca
     9481 aaatggttat ggtcaactcg aacatatttc atttaaaaac gtatgccaag cagttgtcta
     9541 tataagtggt caaaagaaag gcgatgttgt tacgcacttc agagattatc aattacgtat
     9601 acaagataat gcattaactc gacaatttag agatttggtc caagacagtc aaatcgacat
     9661 tgtattagaa gcaaatattc aacaagctat ttggtataaa ttgttagtta acttgggcat
     9721 taattctatc acagcactcg gtagacagac cgttgcaatc atgcataatc ctgaaatacg
     9781 tacactgtgt agacagctat tactagatgg ttgtcgtgtt gcacaggcag aggggttaaa
     9841 cttttcagaa caaactgtcg atactattat gactatatat caaggttatc ccgacgaaat
     9901 gggaacgagc atgtattacg acattgcgca tcagcaacct cttgaagtcg aagcgattca
     9961 aggctttatt tatcgacgtg cacgtgaaca caatcttgac accccatatc ttgatactat
    10021 ttatagcttt ttacgcgcgt atcaacaaaa tatgtaagca catatataat ataaaaagac
    10081 cttaacgatg catttagaac cacctgttcc taatatcgct aaggtctttt aattatatta
    10141 gactattcag cttctctaat ttcttctgcc acatctttat agtctatttt cgctttaaca
    10201 aacgtctcgt tatttaccgg gaaatgttgt tggaatgttt caaagttttg tatctcaacg
    10261 acaacgtcat ccacttcaaa gtcaagaaca tgtccaccat aagctctttc atcatccgca
    10321 aaatgtatat gaaaaccagc agaccctacg ccatgaaata attctggtgt aaaaaatcca
    10381 acaatggcac cacgaatatc ttgtcttttt tcctcaggtt gtctgcgtgc tgaatcaatc
    10441 aaacgtgtat atggcggttg ctgagcaggc atcattcgta catgcatatg tttaaatgtg
    10501 ccataaattt taacagccga aaataaattc tcacttaaca tttcattttt aatttgtgca
    10561 aatacatcat cttgtgataa ttgttgcaat ggaaatgtct tactcgcttt aaaattagta
    10621 atcgatgcat acggtacttt ctcatcgcct tttaattcta taaattcttt atgctcgtta
    10681 gcatgatatg cctttccgtc taaaaatatt acttcgccat cagaccctgt taacgttgca
    10741 atccctaaat tcccatgttc taacaattca ttaattgtag ctgtgccttc tagtaagcca
    10801 gccattaacg tacctaatgt accatgttga tacaagacat tcgtcattcc aaaaccatct
    10861 ccctatagtt attcctcgtt gtcatgctct acttcgtgat taatgcagaa ctactttcct
    10921 ttatactcaa tgacgcctca acatttttat tcatgtcact aaattttcac gagtcataat
    10981 actttattca attattattt tacgcaaaag tattgcctct atcaatgctt gctaaaaaaa
    11041 tggacctatc agtgccacgt gttactgcaa cactaataga tcctcttacc attaaaatat
    11101 ttctaattaa tacttaaaat aggcttagtc ttctaataaa tatttaattg aatcaaatgt
    11161 atcttctaat gttttaacag atttatcgaa caacgcttgt tcttcggcac ttaatggcat
    11221 ttcataaatt ttaactgcgc catgttggtt aactaatgtt ggtacaccta ggtaaacgcc
    11281 tttgtgacca ccatattgac catctaattg tatagaaaca tttaagacat tattttcatt
    11341 atttaaaatg gcttttgaaa tgcgcatcaa tgctaatgca ataccatagt atgttgaccc
    11401 tttagcttgg ataatttcat aagcagcgtc acgtgtattc acataaattt cttccgcttt
    11461 agcttcgcta ccagtttgtt cttttaatgt gtcatatact gaaatacctg ctacatttgc
    11521 ttgtgaccaa actgcaagtt cagtatcacc atgctcgcca ataatactag cgtcaacact
    11581 tgaaggtgca acaccaagtt cttggctaat taaatattgt aaacgtgcac tgtccaatac
    11641 agtacctgaa ccgataacac gctctgctgg taatccagta tattctttta caaatcttgt
    11701 taaaatgtct acagggtttg ccgcgattaa gaaatatcca tcaaagccac tatccataac
    11761 actcttaacg atgctcttca taatcttagt atttttttca actaattgta aacgtgtttc
    11821 acctggcttt tgaggtgcac cagctgtaat aacaactaaa tctgcatctt tacagtcttc
    11881 gtattcacct gctttcacat caactggtga aggactgtgg actgtaccat ggtttaaatc
    11941 ttgaacatct gcttttactt tgtcttttgc aatgtcaata attacaaatt catcagcaac
    12001 accttgcgta accattgcaa aggcatagct tgatcctaca gatccatctc cgattaatac
    12061 aaccttttta ccaaatgttt tcataattat gtctcctaac ctaattgaat ttattttaca
    12121 cgtgactaca cgtaaaaata tctatactga aattaaagat taacaatttt attttattat
    12181 aatattatgc taccaaatta caaacataaa actgagatgt aagcggagtc aaattacact
    12241 ctatcgccaa taaatattga tatctcaagc tttagcacgt aatttgaaat ttgtgacaat
    12301 ttttagcatt ctaaaattgc gtaacgacgc tatttaaagg ctttaacaaa ctattgagct
    12361 ttctattttt ttcattttaa aaaggatggc tattcgactt aaatgactta ctatataacc
    12421 tcctattttc cgccttaccc tcatcatatt tgatgcatca ttttatagtc aaagaattac
    12481 atttcataca aactagccaa tcagacataa acatgaaatt gattttaaaa atataatgtg
    12541 caaaattgtt agaaaattag caaaaaatag tacttattca tttaatatct gttataatca
    12601 gttttatcat ttaaaaaatc tatggatgtt atcaagacaa gtcattaata cgtaacataa
    12661 agttgtgatt tttcagaatt tttagacaaa atattgatta tttgtgctat gatataacat
    12721 attaaaaaac accgtataga attgtttatg aatgtttggg aggaaagttt atgggaagtt
    12781 ttttcaataa aatagcacga aaagaggatc cggctatcta tcaaaataaa gatggtcatt
    12841 taaagcgtac acttcgggtg cgtgatttct tagctttagg tgtaggaaca attgtatcga
    12901 catctatctt tacgctacct ggcattgttg ctgcagaaca tgcaggaccg gccgttgcgt
    12961 tatcattctt actcgctgct attgttgctg gtttagttgc atttacttat gcagaaatgg
    13021 ctgccgctat gccatttgca ggttcagcct attcttgggt caatgtatta tttggtgaat
    13081 tttttggatg ggttgccggt tgggctctat tagctgaata ttttatcgcc gtagcctttg
    13141 ttgcatcagg attctcagcg aatttacgcg gacttgtgaa accaattggc atcgaattac
    13201 ctgcagcatt atcaaatcca tttggtacaa atggcggttt tatcgatatt attgctgcta
    13261 tcgttatttt attaactgca ttattactat cacgtggtat gtcggaagca gctcgtatgg
    13321 aaaatatttt agttatttta aaagtattag ctattatttt atttgtcatc gtaggtttaa
    13381 cagcaataaa tgttagtaac tatgtgccat ttattccaga acacaaagta actgctacag
    13441 gtgactttgg tggatggcaa ggcatatatg ctggtgtttc aatgattttc ttagcgtata
    13501 tcggtttcga ttctatcgca gcaaactcag cagaagcact tgatcctcaa aagacaatgc
    13561 ctagaggtat tcttggttct ttaagcgttg ctatcgtatt atttattgct gtagcacttg
    13621 tgttagttgg tatgttccat tactcacaat acgcaaacaa tgctgaacct gttggttggg
    13681 ctttacgtca aagtggtcat ggtgttgtag cagctattgt tcaagctatc tctgttatcg
    13741 gtatgtttac agcattaatt ggtatgatgt tagcaggctc acgtttactt tattcatttg
    13801 gacgtgacgg cttattacct tcatggttaa gccacttaaa cgacaaacat ttacctaatc
    13861 gcgcactagt tatacttact attattggtg ttttaattgg ttctatgttc ccattcgcat
    13921 ttttagcaca attaatttca gcaggtacac ttgttgcatt tatgttcgtt tcattagcaa
    13981 tgtatcgttt gagaaagcgt gaaggtaaag atctaccaat tcctgcattt aaattacctt
    14041 tatatcctgt gttaccagca attacatttg ttttagtatt gctagtattc tggggattag
    14101 gattcgaagc gaaattatac actttaattt ggtttattgt tggtatcatc ctatatttaa
    14161 gttatggact tagacattct aagaaaaatg acgtagctga ataccaccca ccaaaataaa
    14221 aaataaaaaa aatcatttcc catctgatta acatccagat gtggaaatga ttttttattt
    14281 tatattaaga taatcgctat taccaacctt gtccagatat gtcatattga tctaagttac
    14341 ctgcttccaa agcagtcagt gcatcttcta tagtgtttaa cgccgtgtct aattgctcat
    14401 atgttattac caatggcggt tggaatcgca acacatttcc tgctacagct ataataacta
    14461 cgccatgctc aaagcagtaa ttacaaattt taagtgccgc actggcatca cgtgttttga
    14521 gttttttgtc ggaaacaata tcaataccaa tgctcagacc tttacctcta acatcgccta
    14581 cactattgta tttagatacc cattgatcca ttcgtttcct aacatattac cctttttccg
    14641 cactagcctg aagaagcgac tgatcttcaa tcatttgaat tgtggctaat gcagcttcac
    14701 aactaactgg attagcacct gttgtaaata aatgtgctgg tgcttctaaa caattcataa
    14761 tctctttgcg tccaacaatt gctgacatag gcataccacc tgctaaggat tttccgaaag
    14821 tgattaaatc aggcgtaaaa ttaaaatgcg agactgaact ccatgtacct gttctcccaa
    14881 aaccttgttg aatatcatcg acagcgatta aaataccatg ttcacgacaa atcttttcta
    14941 acgcttcaaa ataccctgga actggttcta aaagtccacc atcgccttgt atcgtttcaa
    15001 taacaataca tgctacttcg tcagcaggta catacttcgc aaacatttct tttaagggtg
    15061 ctaaatattc ttctactgaa ttagcttgtg gctgctcgta cataccacga tatttatctg
    15121 gaaacggaat atgataaaaa ccattcaata acggaccata atgtttgcgc atatttaaac
    15181 taatagctga catagacaat gagccaaaag ttgaaccatg atatgcatta gtgaaactaa
    15241 tgatataagg acgccctgta tatgctctgg caaatttaat gatgccatca tttgcgtctg
    15301 atccggttaa tccgaaggtc actctttttt caaaatctcc aggtgcaatc tcacaaagct
    15361 tcttagctaa acgtaccaac ggttcatgat acatatatgc tggtgtataa tgaataaatt
    15421 tatcaacttg tgcctttatc gcttctgtta cttctctagg tgcatgacct acgttttgag
    15481 aactcgcact cgataacaaa tcgatatatg tcttcccctc aatatcaacc aatgttgctc
    15541 catatccatg atcaatcact aacggataat atttaatacg tcctgatttc gcaaaataat
    15601 gttcatcctc ttgaattaat tgatgtgctt tactcatata cattcccctc ccattgacag
    15661 tattaatgtg caagcaaagt ggcctctctt aaatcgatta taaaatccga tttaaaatcc
    15721 tgctcaaata ccgtcatgcc tctatgaata ctatccaata tactaataaa attcagattt
    15781 gtaaatatat tctgataatt aagaatttta tatcaatcta ctttgtttat gcgcataatt
    15841 ttgttcgatt agggaattgt ttactatatt ccatttgact ctgaggtgat acgaatgcgt
    15901 ttaaaagtat tgttccattt cattgctgct attttcatat cattcatgtt gctatggatg
    15961 accatgttgt ttgacttaat aagcaatcaa tcacatttaa aagctttatt attgaattta
    16021 gattttctaa ttccttctga caatacacca tatattctag aaataatatg tcacttacta
    16081 attggtagcg tcatttattt tgtatttgtg ttgctgtttc atacttctaa aaggctttac
    16141 tatctatgtt atatcccttt atttttctta tttatagcat tgtatccctt tttagtattt
    16201 attgcgcaac gtccaatttt tcaatttagt gtgacagaat taatcggttg gattattaca
    16261 catattttct ttatgtcatt aatggcgtta gtcataccta gaataaaata atatttttaa
    16321 ttaaactgac aattattttt cacattttat acacttttca aggtttatga cttgagaaat
    16381 gttctcattt ttatcatact ggaggtgtat tgaaaagtta aggagtggaa agtatgaata
    16441 aagagcaatt agaaaaaatg aaaaatggaa aaggctttat tgccgcatta gaccaaagtg
    16501 gtggtagtac accaaaagca cttaaagaat atggtgtaaa cgaagatcaa tatagcaatg
    16561 aagacgaaat gttccaactt gttcacgata tgcgtacacg tgtggtaact tcaccttcat
    16621 tctcaccaga taaaattttg ggtgctattc ttttcgaaca aacaatggat cgcgaagtag
    16681 aaagcaaata cactgcagat tacttagctg ataaaggtgt tgttccgttc ttaaaagtag
    16741 acaaaggtct tgctgaagag caaaatggtg ttcaattaat gaaaccaatc gacaacttag
    16801 acaatttatt agaccgtgca aacgaacgtc acatttttgg tacaaaaatg cgttctaaca
    16861 ttttagaatt aaatgagcaa ggtatcaaag acgttgttga acaacaattt gaagttgcta
    16921 aacaaattat tgctaaaggt ttagttccaa ttatcgaacc agaagttaat attaatgcaa
    16981 aagacaaagc tgaaattgaa aaagtattaa aagctgaact taaaaaaggt ttagatagct
    17041 taaatgctga tcaattagta atgttgaaat taacaattcc tactgaacca aacttataca
    17101 aagagttagc tgaacatcct aatgttgttc gtgtcgttgt attatcaggt ggttacagca
    17161 gagaaaaagc aaatgaatta cttaaagata acgctgaact tatcgcaagt ttctcacgtg
    17221 cattagctag tgatttaaga gctgatcaat ctaaagaaga attcgataaa gctttaggtg
    17281 atgctgtaga atcaatctac gacgcatctg taaacaaaaa ctaatttata cattaaacta
    17341 ttttttgata taaactacta aaaaggactc atacatgtgc tttaaaggca ttgtgtatga
    17401 gtcttttttg attaaagttg attttgccgt atgtattaga ttacgtaaaa aaatcggaca
    17461 gctgaaaaat ctttaagatt tctcagttgt ccgattttaa atatagctat tagaatttag
    17521 ttttcgtagt aacctaattc taagtctttt gaagtttgtt tacggatttt tctcattaat
    17581 ttttcgtctt caattaatga ttcaccgtat gatggaatca ttttcttaat tttaggtgcc
    17641 cattcagttt tgtattcagg gaagttacgt tctaatactt ctaacgcaac tgaaactgaa
    17701 gttgaagccc ctggtgattc acctaataat gcaattacag tgtggtcttg tgagttaacc
    17761 acttctgtac cgaattggat gaatccttta ccgtgttcag gtgtatcttt gataacttgt
    17821 acacgtttac cagcagtgta taattgccaa tcttcattac gtgcttctgg atagaaagta
    17881 cgtaagtggt tcatacaacc ttcttttgtc ataattactt ggtcaaatga gtatttaatt
    17941 aaaggtaagt ttttaactgc tgctgctaat aaagttgtaa tgttgtatgt tttaacagac
    18001 ttgaataaat ctaagttaga accatttttc aagaatttag gtccaacatt agcaaatggt
    18061 ccaaataata atgttctttg accatcaatg taacgcgtat ctaaatgagg tacagtcatt
    18121 ggtggtgtac caggtggctc tttaccataa actttggcat cgtgttgttc aataacttgt
    18181 gggtttgtac aagctaagaa ttgaccactg atagggaatc cacccaaatg tttactttca
    18241 gggatacctg ttttttgtaa taatggaatt gctccaccgc cagcaccgat gaatacgtag
    18301 tcagttactt gtttgaattt ctcaccagtt aggcgatttt taacagtaac ttcccattga
    18361 ccatttgata attgttcaaa atcaacaact tcatggttaa attgcactgt agcatttgga
    18421 tgtgcttcaa tgcttttagc cattttacgt gttaattcac cgaagtttac atctgtacct
    18481 tcgtcaattt tacttgccgc catgatacca gggttatctt cacggccttt catcatcaat
    18541 ggaatccatt ttttcattac ttcgatgtct tcagtatatt cgatattatc gaacataggg
    18601 aaagctttca tcgcttcgta acgatctttt aagaatttaa cattgttttt acctctaaca
    18661 taactgatgt gtggtaatgg attgataaat tctcttgggt tctcgatgct accgcttttc
    18721 actaagtgac cccagaattg ttttgaaatc tcaaactctt cgttaatcac tttcgctttt
    18781 tcgatgtcga tagaaccatc aggttgtaaa actgtgtagt tcaactcaca taatgctgca
    18841 tgacccgtac cagcattatt tctttcgttt gaactttcga ttgcaggacg atccaagcgt
    18901 tcgtaaacgt ggatattcca gtctggctca atttctttta acattgaacc aaatgttgtg
    18961 ctaagtacac cggctccaat taaaacgatg tctttactat tagacttagc cattggtttc
    19021 acctctccaa aaattgtaaa tgtgtatcat caatatgaaa gttacataaa actgacatat
    19081 ttctttaaaa tatcaacgcc attgataact tcctgtttta attgatacgc tgtaacaaaa
    19141 tactatagtt agtgcttaca tgtatatgtt aaagcaagca gtggtaaatg taaattataa
    19201 ttattcatta actttgcaat atattaaatc ttttattcat agaagataaa tatcaaatca
    19261 atcataatta tttgacaaca aatagctaac gattgtttta atctacattt ggcttatagc
    19321 attttaaacc tatactctat tttgatacaa tataagtgta aaatcaatca taaaaaggat
    19381 attcaatatc tgcatccaag aaaaacatta caattacctt taatattgaa aattattttg
    19441 aatcttgcac ttataggtat tgtaattatc gttctaacac atactgagtt tcaacttaat
    19501 gccggtgata gtaatgtgtt tttattaaac tgggatattc gaggtttaac atcttatatt
    19561 ttctcattct taatactggt tgcgcttgtt tctaaactaa ttttaatatt tataactaga
    19621 cgacaaagaa catcataaat aaacaagcag tccagtgcgt atcatcatac cttacgcatt
    19681 ggactgcttt gctatgttta ttgattgcta tctttttgtt gttgccattt ttctatttca
    19741 gcatctcgta gttcaacacg acgaatttta cctgagtttg tttttggtaa gtcgtcaacg
    19801 aattcaatct ctctcggata tttatatggt gcaacttcat ttttaacaaa ttgttgtagt
    19861 tctttaacta acgtatcatc acccgcagta tggtcctgta aaataacgaa tgctttaaca
    19921 atatttcctc gtatatcatg aggttttgct actacagcac attctttaac ggctggatga
    19981 ttcgttaacg catcttcaac ttcaaaaggc ccaatcgtat agcctgaact aataataatg
    20041 tcatctcgac gtccttcaaa ccagaaataa ccatcatcat ctacatgagc taagtcacca
    20101 gtgatgtagt atttacctgt ttgcgctttc gccgtacgtt ctggctcttt ataataccct
    20161 ttgaaaagtg ctggcaaatc aagtggtact gcaatattcc ctttcgtatt agcaggtacg
    20221 ctattcccct catcatctac tacagtgacc gaactacccg gaatgccttt acccattgat
    20281 ccaatcctct gtggtgtatc ttttaaaaag cctataagca aggtactttc agtctggcca
    20341 tatccatctc ttacagttaa attaaagtat ttcttgaatt gttcaactac ttctcgattt
    20401 agtggctcac ctgcagaaac ggcactatgt aaatgcgtta agtcataatc atttaagttc
    20461 tgtaatttag ccatcatacg atattctgtc ggtgtacaac ataaaacatt aatttgatat
    20521 ttttgaagca attctaagta tgtttcagga ctgaaccttc cattaaatac aaaagcagtt
    20581 gcacctgaac ctaatacaga taagaaagga ctccataccc atttttgcca acctggtgct
    20641 gctgttgccc aaactaagtc atcttcatta atacataacc aatgttttgg tgccatttgt
    20701 aaatgtgcaa atccccatcc atgacaatgt gtaacggctt taggattgcc agttgtacca
    20761 gatgtatatg acagaatcgc catatcatca cgcgtcgtat ctgccatttc tagtttgtta
    20821 cttgcgtttt ctttttcagc ttcaagtgaa atccatccat ctttttgacc ggcaataaca
    20881 aatttagtta acgcatcata ttctttaatt ttttcaaatt caactgtgaa tggctctagt
    20941 gcaataactg cattaatttc accatgtgtg atacggtatt gtaaatcttt agttcttagc
    21001 atttcagaac atggaatgat tgcaacacct aattttaaag cagcaatata taattcatac
    21061 gtcgcaatag atcgtggcat cataatgagt actttatcgc ctttagataa accgtgcgat
    21121 gctaaaacat tacctacttt attagactgt tcaatgagct gttggtaagt gactgatata
    21181 tcttcgcctt cagtattatg atataaaatt gcctttttat ctggtatgtg gctatatttt
    21241 tcgatttccg aaataatgtt atatttttca ggcgcgaata gagctgactt ttgcataact
    21301 aacttccttt catacatcca cttttcctgt gatgaacatt gtaattttat aaatgaatta
    21361 tatacatcat acgcctatct ttacagaatt ttcaattaaa tagggttaaa taccaaagtc
    21421 ctcgacacta cactttgaca tgacgtagca ttcaaggact ttcaaatgat tgagggttga
    21481 tatctcgggc tagaccatat cagctaattc aatacgaata ttgtatgata attcacgatt
    21541 aattattttt acatctgcac ctttcgaagt gccacgatgc ttgtgtgtat gcttgtactc
    21601 agctgaattt tgccaatgat aaaatgcttg cctattttcc cacagcgtaa taatgatata
    21661 gtgtctacca gctgttctag gtcttaaaaa ccttaatgct ttaaatccat caacgttttt
    21721 taaatgctta ttacgtcgca aaaatttttg ctcgaatgtt tcttcttgtc cttcgttaat
    21781 atataaatga tttaacacac ataatgaatc atttgataaa tcatttatcg cttctagcac
    21841 atcgtaatat gcagtgtcat tatttttttg tattgtgaga caatcatcca gttcttctat
    21901 tacataactt ctatattcat cataaatttt cataataaat gccttcattt catttatatt
    21961 tttggtcata ttactttata tctattacta attcattccc gtatttatta attacaatca
    22021 tagtttggct tctttttaaa agataagact ttgtaaaaag tattaatatt tcatgcaaat
    22081 gggggacagg agtcgcccac tatttttgtg tcttcaattt catgatcatt atttaacatt
    22141 agtcatgaaa atagccgaca acttcatctg tgaaatcacc ggccttttat tttagctaac
    22201 tttatttctg attttacgat tttaattgat catacagaga aagtgatctt tttacaattt
    22261 ctaaaaactc atgatctata ttggacattt gatgaaaata agacaaaatg ttttctgtta
    22321 gcttctcttg ttttgggaat gaatcatctt ctttaatcca aatcgctaat tcgcctaatg
    22381 gtgttttatc atctttaaat gtttgtatat attcgtaaaa gctcatagta ttccttctct
    22441 caatttactt atataaatcc taccacgaaa gctttcaaga aaacacaatt aaatgtctat
    22501 ttagtgaact ttttaaggtt gtgcactctt ttaatgtctg ccaattaggt caattaatca
    22561 tcacaatgta caattaactc tattttcagt tcatatactc acacaccgtt tttgaacaac
    22621 acattaactt ctcatttaga taaaacgcaa aaaagcctgg caccaataca atagatgcca
    22681 gactaagagt ctactatata aatttattta gcgtatggtt ttacttcgat tgcaccttca
    22741 ttttcatcat gaacaccatg cttataataa tcaatatatt gtggctctaa aggctttctg
    22801 ccacgtataa tgtctgctgc tttttcagct aacattaaaa caggtgcgtg tatattgcca
    22861 tttgtcgtac gtggcatagc tgatgcatca actacacgta aattttccat accgtggact
    22921 ttcattgtta acgggtcaac tactgccatt ggatctgaag caggacccat tttagcacta
    22981 caagatgggt gtaatgctgt ttcaccatct ctacgaaccc aatcaagaat ttcttcgtct
    23041 gtttgcactt ctggtcctgg tgaaatttct ccaccattga atggatccat tgctttttga
    23101 gataagatat ttcttgctac acgaattgct tctacccatt cttttttatc ttcttctgtt
    23161 gataaataat taaagcggat acttggtttt tcgaatggat ctttagattt gattttcaag
    23221 ctaccacgag agtttgaata cattggtcct acgtgaactt gataaccatg tgcgaccgct
    23281 gccttttgac catcatatct tacagctatt ggtaagaaat ggaacattaa gttaggataa
    23341 tcaacttcgt tatttgaacg tacaaatccg ccaccttcaa aatggttaga tgctgctgca
    23401 cctgtacgtg tgaaaatcca ttgtaaacca ataaatggca tgcgcttgat atctaagctt
    23461 ggctgtaatg atacaggttc cttacattta tgttgaatgt atacctctaa gtgatcttca
    23521 aagttttcac ccacaccagg taaatgaaca cgtggctcaa tgccttttga ttttaggaac
    23581 tctgaatcac cgataccaga taattgtagt aattgtggcg tattgaatgc cccaccagac
    23641 aaaatgactt cattagcatc gatggtatgt agtttgccat ttttcttata cgtaacacca
    23701 gttgctcttc taccttcata atgaatttca gttacaaagg cacgtgtttc aacggttaag
    23761 tttttacgct tcatcgctgg atgtaaatat gctcttgaag ctgacattcg gcgaccacga
    23821 tgtacttgac tatcgaacgg tccaaaacct tcttgtctaa atccattcac atcaggtgtt
    23881 ttatgatagc ctgcttcaac acctgcatca aagaatgact ggaataaagg attcgttgct
    23941 ggccctcgtt ttaacttaat tggtccatca tggcctctaa atttatcata aggcgctgca
    24001 ccgtatgttt tttctaattt tttaaaatac ggtaaacagt gcgcaaaatc ccaagtttcc
    24061 ataccttctg gttctgccca gccttcatag tccattggat taccacgttg ataaatcatg
    24121 ccattaatcg aacttgatcc acctaaaact ttacctcggg catgtgctac tttacgaccg
    24181 cccatatgtg gttcttcatc tgttgaataa atccaatcgt aaaatttatt gcctgaaggg
    24241 aacattaacg cagcaggcat ttggataaat aaatcccaaa aataatcact gcgacccgct
    24301 tctaatacta agacttcttt atctttatct tcactcagac gattacctag tacagaacct
    24361 gcactgcctc ctccaatgat gacataatca tatgatttgt ttttgttact catcgttatg
    24421 acctccaaag tttgttgtat ttcaaagtga gtattgcatg aaatatttgt tagtggctga
    24481 ttggttatcg ttgaattgct aaacttagct aaccaaaaca ctcgcttttc cttttacgat
    24541 tgttactaac agtaatgaaa taatctatga ttcattttgc gtttagtcca aattatcaat
    24601 caacatttcg ccacttatac aaaacaaagt gagtgtcttg tgttcttaca atggcactca
    24661 ccttatctaa tttttatttg ctaaaccaat tcactaattg tggatttgta tttgttaaaa
    24721 tgtgttttga aacaaggtac tcttctaagc cttctttgcc taattctcta ccgatacctg
    24781 attgtttgta tccaccccat ggcgcttgtg caaaatatgg atggaaatca ttaatccaca
    24841 ccgttccaag tttcaacttg ttagcaacgc gttgtgcttt tccaatatct ttagaaaata
    24901 cagcacctgc taaaccatat atagaatcat tcgctaattg aatcgcttct tgttcagttt
    24961 caaagccttc tacagtaacg acaggtccga aaacctcttc ttgtacaata cgcattgacg
    25021 tatcacaatt tgtaatgact gttggctcga agaatagacc atcttttaaa tcatctctat
    25081 ctggacgttt accaccaaca gcaattgttg cgccttctgc tttagctaca tccatataag
    25141 attcgatctt attacgatgt tctgttgaaa tcactggtcc catttcagta tcagcatcaa
    25201 aaccattacc taatttgatt tttttcacgc gatcaataag tgcttgctca aatttgtctt
    25261 taatactgtt ttgtactaat attcttgatc ctgctgaaca aacttgacct gcatggaaat
    25321 atccaccatt taacgcttgg tctactgcca attcaaaatc agcatcatca aagataatgt
    25381 ttggattttt accgccaagt tccaaggcaa tattcgtaac attattagca gcatttttca
    25441 taatatgctt accagtctca atgccacctg taaatgatac aaggtcaacc tctttatgac
    25501 ctgacattac gtcaccaact tcagaacctg cacctagaat aagattaatt gttcctttag
    25561 ggaaaccaac ttcttccatt aattcaaaaa cacgtattgt tgttaatggt gtaatttcac
    25621 ttggtttcat aactagtgaa caacccgtag caagcgctgg cgcaattttc catgatgctt
    25681 gtaataacgg ataattccaa ggtgtaattt gtgtaactac acctactggt tctttaacaa
    25741 ttttgctttc tgtatctgga attggtgaat caatcatttc gccaccgtct ttatctgcta
    25801 atccagcaaa atacataaac acattatgaa tatcatccat atctgcatat gattcttcta
    25861 acgtttttcc agtatctaat gtttctaatc gtgctaacgc ttcgcgatgt tctttaatct
    25921 tgtccgcgat ggcacgtact tttttacctc ttgtttcagc agtttcttgc gaccattcac
    25981 cagactcaaa cgcacgtctt gcagctaaga ttgcacgttc tgcatcctct tttgtccctt
    26041 cagaaaccgt aaatatcact tcttgattgt aaggattgat aatatctctt gtatttttat
    26101 tcgcgctttc aacccactca ccatcaatat attgacgctg agataaatgt tttaaaagtt
    26161 ccattcaaca acctccgtta agtttgttaa atttttattt aacgaatttt acaaacttat
    26221 gttagcatag aaatgaaaaa tgtgtcaatt tacaaaacaa ctcaagaatt aagttttgta
    26281 agttcgttag atttttattt aacgaaacat ttaactttaa tgctttaaat atttttaatg
    26341 tcttttttta gaaaaggttt tataatgtat tagtatttat tttaacgtga ccaaaatgat
    26401 gtacggaggt gcaatgatga ctcgttctca gaacaaacag caacaattag aacaagcaaa
    26461 agatattgta attaattcaa ttggacagac aatggatttg tatggcacta atagaagtgt
    26521 aggaaactta tatggcacaa tggtgtttga aggcagtatg acgcttgatg aaatgcgtca
    26581 tcaattgcaa atgagcaaac caagtatgag tgctggcgtt aagaaattgc aggaatttga
    26641 tattgttaaa caacaattta cgagaggaag ccgcaaacaa cattttatcg ccgagaaaga
    26701 ctttttcatc tttttccgaa actttttcac taaaaaattc cagcgtgaaa ttgacatcaa
    26761 tgtcgaagca gtaaaagatg ctcaagctat tatcaattca ttacttgaaa gcagtgattt
    26821 aactgaagct gaaacacaag aggcacaaaa aattaaatct cagcttgatc atacacatgt
    26881 ttattacgaa tggttagaac agttaaccga agcgattgaa agcggtgaaa ttttcaaata
    26941 tttcccaatt ccagaacagc cttctgattc tgaaaattaa aattgactca aacaacgaca
    27001 atgatgatta tgcatgataa aaacaatgca ataggtccac gaaataaacg gtaatttcta
    27061 tctcaataaa tagaaattac cgtttttgtt ttatatgaca atcaattatg gaggtatttt
    27121 ctagggcttt cttagattcg ttacattgct tcgccaatat atttcaatta gctattctcg
    27181 ccctcacgta ctctatcaat aatattttgt atttgttcac gtttttcata ttcattttct
    27241 acaaaataaa ctaacggttc atctttgata gtaacccaag gtacaagtac atcaaaatct
    27301 aattctttaa ccatctctaa actttgaata gatttttctc tatcacttga acctaatata
    27361 actgtacgcc atctcttccc ttcaaaacat ataaaatctc cagtaaataa gtaacggtga
    27421 tgaccatcat cccataaaaa tagtgtcgtc cctggtgtat gtccaggcgc gggaataact
    27481 tctaaatcct tatccaacat tacacgatta ctaaattgtt tttgaacaga aatcttatgt
    27541 ttcagtgcag ccacatcatt ttcatgtata aagtaagggg cttcaacttg attagaacct
    27601 cctatagatt catgatcatg attcatcaat acttttgaaa caccacctaa tgatgcaatg
    27661 tcttgttggg agtcacctaa gtgtccggaa tgatagataa caatattacc tgtttcacgt
    27721 tttaaaataa aagatttaaa tagaaatcga ttatcaaaag gcagttccga agtaggtgtc
    27781 gcatataagt tttttgtgat ggattctaaa ctgtcatgta atttggactg tttattttta
    27841 aattgattag tcattttatt tcatccttcg attaatttga attgtacata cgcagtaccc
    27901 tttatttaat ttaataatca atgagagtct ttcaaacttc gatttagaat atgcttcata
    27961 caataaataa gtacttggag cactaaaaaa ttaaatcttt tagtacaaca ttaatgaaaa
    28021 cctaaaagtt catcctacaa tgctactaaa aaaaggggaa tggaacagaa atgatatttt
    28081 cacaaaattc atttcgtcgt cccagacccg ctttgaatta taaattatcg tcttgttctt
    28141 cttctgatac ttgaacgatt cgcaatgaac gacgttcaac ttcttttaat ttttcagcac
    28201 gcgtttcaag tttaattcta tcgcgcccta aaatgattaa aaatgatatc atcatgaaaa
    28261 taaaaataac aattaatggc acacttgcca gtattgaagc agttttcaat acttctaatg
    28321 cacgttcacc accaactagc atcaatgaaa atggcaataa gcacaatgca aatgcccaga
    28381 ataaacgatt ggcacgtaat ggttcgccta ccactttttt ctgagatgct gccgctaaaa
    28441 tatatgaacc cgaatcaaat gttgttgcta agaataagaa agcagatact aagaatagta
    28501 caatcatcaa tgatgggaat ggtaaatgat gcaccacttc aataatggtt gcctctgtac
    28561 catgtgtatt taaatattgt gttacattaa actgtccaga aatttgtaaa tacacagcat
    28621 agttaccaaa aataccaaag aataatacgc atccaagcgt tccataaata attgttccta
    28681 gcacgacttc tttaaggcgt cgaccttttg aaattctagc gataaataaa ccgataaatg
    28741 gcgcatatac taaccaccat gaccagtaga atattgtcca gtcttgtggg aaattcgttt
    28801 cttttcgacc tttaatacca ccgaatggtt ctaaccatgt tgccatatga aagaaatctc
    28861 tcaacatatt tccgaaccct gtcactgtcg tttccataat aaaaacagtc ggtccaataa
    28921 taaatataaa ggctaaaagt acaaaggata gccaaacgtt gatatcactt aacttttgaa
    28981 tacctttttt caatcctgta tatgaactaa tggcaaatat aaccgtgatt gttaataaaa
    29041 tggccgaacg taaaatcata tttttaccat ctaaaccagt taatctttct atgcctgcag
    29101 aaattaatgg cacacctaac gctagtgatg ttgccgcacc acctagcaat ccaaagataa
    29161 ataagatatc tacaacttta cctacaaatt tatctgtttg accttttaaa atcggacgac
    29221 aagcttgact aattttatac accggttgtt ttttaacaaa tactaaataa ccaattggta
    29281 atgctggtag aacataaata gcccaagcaa ttggccccca gtggaacata ccatattgcg
    29341 tcgcatattg gagtgcttca tcactcatac ttttcgcgcc atttggtgga acttgatagt
    29401 aaaaagccca ttcaataacg ccccagtata aaatatcaga gcctatgcct gcacaaaaca
    29461 gcattgccgc ccatgtaaat gtattaaatt ctggtttatc acttgcttta ccaagtgtga
    29521 cattaccata tttaccaaat gcgatataca ttacaaagca aaaaatcgcc agccccataa
    29581 ataaatatat cgaaccaatt gaatcagaaa tggcactatt aataccagtg atgatatctt
    29641 cacttgcttt tggaaaagcc atcataggta taactgcaaa aagaagtaca gctactgtcc
    29701 ctataaaggt cgtccagtcc ataactttct cttttttcaa ttgtgctccc cctaattatt
    29761 aattttatga atcctgtttc gatttatctc aaaatgtaat aattatattg attcacaaaa
    29821 ttgacaataa ctaacattta ataataatgc aaatttcata caatttgaaa cttggcaatt
    29881 attgaatatt tatataattt ttcccgtaat aaacaaaacc ttaatagcgc taaaataaca
    29941 gtgttaagtt acgatttaac gaatttaaca aattttacta gaatggcatt taagaatatt
    30001 tatacgttat taacgaatat ttatttattg taaaacgcta ccaaaaagtt agacttcctt
    30061 cccactaaaa taccactttt ctctttcaac ttttttaaaa aacggatatg caacttttag
    30121 tattggtatc aaaatgattg ttaggtcata ttctatcaat atatttttat aaagaattgc
    30181 ttttattaac tttcaattat gtacctaacc taaaaagaag ccaaggcaac gaatgttacc
    30241 ttgacttcta atacatattc aactaactat atattcaatc atacgcgcat gcgagagtga
    30301 ttgttgtaca tctataatgc gttgatttaa agaaccttta tatggtaaat caggtttgaa
    30361 taagtgttgt ataaatagac catctactaa aacgtcaatg tatgataata actctcgacg
    30421 ttctgtacaa tcatttgcta aatattcata taaaaatcca gtccataccc aaattgtctt
    30481 tgtatttcca aaacgtgctc gaaatgcttt gacaagattt aatgtaatat ccaaattaca
    30541 aaatggttcg ccacctaata gacttagccc agatatataa tcatgatcgc aatcatctaa
    30601 tatttctgct aatatttcat cagtgtattt ctcgccatat ctgaactttt gtgaggcttt
    30661 gttataacat ccaacacaat taaatggaca tcctgataca taaacactgc atcttactcc
    30721 ttcaccgtca acaaagctat ttgattctat tttagcaata taaccttgtc cttgtttaat
    30781 gtctaaaagt atcattcttt aggcgctttc atatgtttta ctcgtgcgca aatttcttta
    30841 tgacggcctt taattactgg acgttgaact ggattgccta ggtaaccaca tgttcgttta
    30901 acgacatcaa ctgttttagg attatcattg ccacagttcg ggcatttaaa tcctttttca
    30961 gttgcttcaa aatctccatc gtaatcacat tcataacaat gatcaatcgg aatatttgta
    31021 cctaagtaac caactttgtc ataagagtag tcccataccg cttctagtgc tttcaaattg
    31081 tgttgcaatt tcggatactc acaatagtga atgaaaccac cactcgcata ataaggataa
    31141 tctttttcaa aatctaactt ttcaaaaggt gtaacatctt tacgtacatc ataatggaaa
    31201 gagttttgat aatatccttt atctgtaatg tctttaatat ctccaaatct ctcttggtct
    31261 aaacgacaaa aacgatccgt tagcgattca ctcggcgtac tgtaaatact gaaccaaatg
    31321 tcatataatt ctgtccattt cgtttgataa cgtttcattt ctttaagaat ttcaagcgta
    31381 aatgcttttg cttcttgaga tgtttcccag tctggaccat agaaaacagt agctgtttca
    31441 tacaacccta tatagcccat tgaaatcgtt gcacgtttat ttttaaataa ctcagcaaca
    31501 tcatctgttt cttttaattt atagttaaat gcgccacttt tatataaaat cggtgcgtta
    31561 ttcggtacag catctttcaa acgatttata cgataaagta atgcatcatg taacacatcg
    31621 atacgttcat aaaagatttc ccagaatttc gtcatattac cggcagattc taatgccatt
    31681 ctaggtaaat taagtgtaac aacaccaaga ttacaacgac cattattttc aaaatgacct
    31741 tccgcatctt tccaacttgg taaaaatgaa cgacaaccca ttggcgcttt gaaatcacct
    31801 aatatttcta cgagtttgtc ataatttaaa atatctggat acatacgttt cgttgaacac
    31861 tttaatgcta gttgtttaat gtcatagttc ggatcttgcg gactaaagtt ggttcctttt
    31921 ttaattgaaa atacaagttt cgggaaaatc gctgtcgtgc ggtcttttcc taagcctttg
    31981 atacgagtat ttaagatagc ttgttgaatt ttgcgactta aatgatctgt acctaagccg
    32041 aatcctaatg ttacaaaagg tgtctgtcca ttagatgtat ataaggtatt aatttcatat
    32101 tctaaacttt caatcgcatc attgatgtct ttagtgactt gttgatcaac ataacgatca
    32161 atttcagatt ctttgacaaa ttgctttgcg atattcctat gttgttcttc attatgtcgt
    32221 gcatatgtac taagtaattc gtcaacgcgg tcaaccgtac agccaccata ttgactgcta
    32281 gaaacattgg ctataatttg tacaagctgc gctgatgcag tttgtattga ttttggtgaa
    32341 gttacattcg cgttgcctat ttcaaatcca ttatgtagca tatttttagc atctattaaa
    32401 caacagttag ttaacggttg gaatggatga taatctaagt cgtgaaaatg tatgtcccct
    32461 ctttgatgtg catctgcaac gtgctttggt aataaatgat ttaatgcgta agatttagaa
    32521 acgatacctg ctgttaaatc tctcattgtt gaaaatgtat cactatcttt gttagcattt
    32581 tcgttaacaa tagcaggatc tttagaaatc aaacccgtta atgcttcttc gatttgattc
    32641 attttacccc ttcttcctat atattgtgtc tactcattac gttataccac tatat
//
LOCUS       DANJFZ010000020        31945 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.020, whole genome shotgun sequence.
ACCESSION   DANJFZ010000020 DANJFZ010000000
VERSION     DANJFZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.020"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            249..7064
                     /gene="sasA"
                     /locus_tag="PWN45_000813"
     CDS             249..7064
                     /gene="sasA"
                     /locus_tag="PWN45_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDL0622596.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            7194..8405
                     /gene="secY2"
                     /locus_tag="PWN45_000814"
     CDS             7194..8405
                     /gene="secY2"
                     /locus_tag="PWN45_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDL0622597.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8416..9969
                     /gene="asp1"
                     /locus_tag="PWN45_000815"
     CDS             8416..9969
                     /gene="asp1"
                     /locus_tag="PWN45_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDL0622598.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9959..11527
                     /gene="asp2"
                     /locus_tag="PWN45_000816"
     CDS             9959..11527
                     /gene="asp2"
                     /locus_tag="PWN45_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDL0622599.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11505..12464
                     /gene="asp3"
                     /locus_tag="PWN45_000817"
     CDS             11505..12464
                     /gene="asp3"
                     /locus_tag="PWN45_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDL0622600.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12454..14844
                     /gene="secA2"
                     /locus_tag="PWN45_000818"
     CDS             12454..14844
                     /gene="secA2"
                     /locus_tag="PWN45_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDL0622601.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14862..16370
                     /gene="gtfA"
                     /locus_tag="PWN45_000819"
     CDS             14862..16370
                     /gene="gtfA"
                     /locus_tag="PWN45_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDL0622602.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16363..17721
                     /gene="gtfB"
                     /locus_tag="PWN45_000820"
     CDS             16363..17721
                     /gene="gtfB"
                     /locus_tag="PWN45_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDL0622603.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17963..19870
                     /gene="sasF"
                     /locus_tag="PWN45_000821"
     CDS             17963..19870
                     /gene="sasF"
                     /locus_tag="PWN45_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDL0622604.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            20038..20598
                     /locus_tag="PWN45_000822"
     CDS             20038..20598
                     /locus_tag="PWN45_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0622605.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20865..22724)
                     /locus_tag="PWN45_000823"
     CDS             complement(20865..22724)
                     /locus_tag="PWN45_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDL0622606.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22935..25916
                     /locus_tag="PWN45_000824"
     CDS             22935..25916
                     /locus_tag="PWN45_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL0622607.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(26028..26966)
                     /gene="manA"
                     /locus_tag="PWN45_000825"
     CDS             complement(26028..26966)
                     /gene="manA"
                     /locus_tag="PWN45_000825"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL0622608.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26980..28932)
                     /locus_tag="PWN45_000826"
     CDS             complement(26980..28932)
                     /locus_tag="PWN45_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDL0622609.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(29009..30883)
                     /locus_tag="PWN45_000827"
     CDS             complement(29009..30883)
                     /locus_tag="PWN45_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0622610.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(31123..31581)
                     /locus_tag="PWN45_000828"
     CDS             complement(31123..31581)
                     /locus_tag="PWN45_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622611.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT        11070 a         5055 c         5957 g         9863 t
ORIGIN      
        1 aaaacgaaac aattataatg attaaacata aagccaatta gccaaattta tataaaaaaa
       61 gattatacgc ataaacgaga aaaagtaatt ttaaaaatat gaaaacgatt acatttagac
      121 gaataaaaat gaatttttac aaattttaac aaaatgagta tttttgtatc aaaaaagagt
      181 tgtattaaat tgaatagtgg catatattat aatctaatga atgtaaagat aatacaagga
      241 gttattacat gagtaaaaga cagaaagcat ttcatgacag cttagcaaac gaaaaaacaa
      301 gagtaagact ttataaatct ggaaaaaatt gggtaaaatc cggaattaaa gaaatagaaa
      361 tgttcaaaat tatggggcta ccatttatta gtcatagttt agtgagtcaa gataatcaaa
      421 gcattagtaa aaaaatgacg ggatacggac tgaaaactac ggcggttatt ggtggtgcat
      481 tcacggtaaa tatgttgcat gaccagcaag cttttgcggc ttctgatgca ccattaactt
      541 ctgaattaaa cacacaaagt gaaacagtag gtaatcaaaa ctcaacgaca atcgaagcat
      601 caacatcaac agccgattcc acaagtgtaa cgaaaaatag tagttcggta caaacatcaa
      661 atagtgacac agtctcaagt gaaaagtctg aaaaggtcac ttcgacaact aatagtacaa
      721 gcaatcaaca agagaaattg acatctacat cagaatcaac atcctcaaag aatactacat
      781 caagttctga tactaaatct gtagcttcaa cttcaagtac agaacaacca attaatacat
      841 caacaaatca aagtactgca tcaaataaca cttcacaaag cacaacgcca tcttcggtca
      901 acttaaacaa aactagcaca acgtcaacta gcaccgcacc agtaaaactt cgaactttca
      961 gtcgcttagc tatgtcaaca tttgcgtcag cagcgacgac aaccgcagta actgctaata
     1021 caattacagt taataaagat aacttaaaac aatatatgac aacgtcaggt aatgctacct
     1081 atgatcaaag taccggtatt gtgacgttaa cacaggatgc atacagccaa aaaggtgcta
     1141 ttacattagg aacacgtatt gactctaata agagttttca tttttctgga aaagtaaatt
     1201 taggtaacaa atatgaaggg catggaaatg gtggagatgg tatcggtttt gccttttcac
     1261 caggtgtatt aggtgaaaca gggttaaacg gtgccgcagt aggtattggt ggcttaagta
     1321 acgcatttgg cttcaaattg gatacgtatc acaatacatc taaaccaaat tcagctgcaa
     1381 aggcgaatgc tgacccatct aatgtagctg gtggaggtgc gtttggtgca tttgtaacaa
     1441 cagatagtta tggtgttgcg acaacgtata catcaagttc aacagctgat aatgctgcga
     1501 agttaaatgt tcaacctaca aataacacgt tccaagattt tgatattaac tataatggtg
     1561 atacaaaggt tatgactgtc aaatatgcag gtcaaacatg gacacgtaat atttcagatt
     1621 ggattgcgaa aagtggtacg accaactttt cattatcaat gacagcctca acaggtggcg
     1681 cgacaaattt acaacaagta caatttggaa cattcgaata tacagagtct gctgttacac
     1741 aagtgagata cgttgatgta acaacaggta aagatattat tccaccaaaa acatattcag
     1801 gaaatgttga tcaagtcgtg acaatcgata atcagcaatc tgcattgact gctaaaggat
     1861 ataactacac gtccgtcgat agttcatatg cgtcaactta taatgataca aataaaactg
     1921 taaaaatgac gaatgctgga caatcagtga catattattt tactgatgta aaagcaccaa
     1981 ctgtaactgt aggcaatcaa accatagaag tgggtaaaac aatgaatcct attgtattga
     2041 ctacaacgga taatggtact gggactgtga caaatacagt tacaggatta ccaagcggat
     2101 taagttacga tagtgcaacg aattcaatca ttgggacacc aacaaaaatt ggtcaatcaa
     2161 cagtgacagt tgtgtctact gaccaagcaa ataacaaatc gacgacaact tttacaataa
     2221 atgttgtgga tacgacagca ccaacagtga caccaatagg agatcaatca tcagaagtgt
     2281 attcaccaat atccccgatt aaaattgcta cgcaagataa cagtggaaat gcggtgacga
     2341 atacagtgac tggattgcca tccggactaa catttgatag tacaaataat actattagtg
     2401 gtacaccaac aaacattggt acaagtacta tatcaatcgt ttctacagat gcgagcggta
     2461 acaaaacgac gacaactttt aaatatgaag taacaagaaa tagcatgagt gattccgtat
     2521 caacatcagg aagtacacaa caatctcaaa gtgtgtcaac aagtaaagct gactcacaaa
     2581 gtgcatcaac gagtacatca ggatcgattg tggtatctac atcagctagt acctcgaaat
     2641 cgacaagtgt aagcctatct gattctgtga gtgcatctaa gtcattaagc acatctgaaa
     2701 gtaatagtgt atcaagctca acaagcacaa gtttagtgaa ttcacaaagt gtatcatcaa
     2761 gcatgtcgga ttcagctagt aaatcaacat cattaagcga ttctatttca aactctagca
     2821 gtactgaaaa atccgaaagt ctatcaacaa gtacatctga ttcattgcgt acatcaacat
     2881 cactcagtga ctcattaagt atgagtacat caggaagctt gtctaagtca caaagcttat
     2941 caacgagtat atcagggtcg tctagtacat cagcatcatt aagtgacagt acatcgaatg
     3001 caattagtac atcaacatca ttgagcgagt cagctagcac ctcggactct atcagtattt
     3061 caaatagcat agccaactct caaagtgcgt caacaagcaa atcagattca caaagtacat
     3121 caatatcatt aagtacaagt gattcaaaat cgatgagtac atcagaatca ttgagcgatt
     3181 cgacgagcac aagtggttct gtttctggat cactaagcat agcagcatca caaagtgtct
     3241 caacaagtac atcagactcg atgagtactt cagagatagt aagtgactct atcagtacaa
     3301 gtgggtcatt atctgcatca gacagtaaat caatgtccgt aagtagttca atgagcacgt
     3361 ctcagtcagg tagtacatca gaatcattaa gtgattcaca aagtacatct gattctgata
     3421 gtaagtcatt atcacaaagt actagtcaat caggttcaac aagtacatca acgtcgacaa
     3481 gtgcttcagt acgtacttcg gaatcacaaa gtacgtctgg ttcaatgagt gcaagtcaat
     3541 ccgattcaat gagcatatca acgtcgttta gtgattcaac gagtgatagc aaatcagcat
     3601 caactgcatc aagtgaatca atatcacaaa gtgcttttac gagcacatct ggttcggtaa
     3661 gtacttcgac atcgttaagt acaagtaatt cagaacgtac atcaacatct atgagtgatt
     3721 ccacaagctt aagtacatca gagtctgatt caataagtga atcaacgtca acgagcgact
     3781 ctataagtga agcaatatct gcttcagaga gcacgtttat atcattaagt gaatcaaata
     3841 gtactagcga ttcagaatca caaagtgcat ctgccttttt aagtgaatca ttaagtgaaa
     3901 gtacgtctga atcaacatca gagtcagtga gtagttcgac aagtgagagt acgtcattat
     3961 cagacagtac atcagaatct ggtagcacat caacatcatt aagtaattca acaagtggta
     4021 gtacgtccat ttcaacatcg acaagtatca gtgaatcaac gtcaacgttt aagagcgaga
     4081 gtgtttcaac atcactgagt atgtcaacga gtacaagttt gtctgactct acaagtttgt
     4141 caacatcatt aagtgattcc acaagtgata gtaagtctga ttcattaagt acatcaatgt
     4201 cgacaagtga ttcaatcagt acaagtaaat ctgattccat tagtacatcc acatcattaa
     4261 gtggttctac aagtgaaagt gaatccgact caacatcatc aagtgaaagt aaatccgatt
     4321 caacatcaat gagcataagt atgtctcaat caacatcagg aagtacaagt acgtcaacga
     4381 gtacaagttt gtctgactca acgagtacat cattgtcact aagtgcctca atgaatcaaa
     4441 gcggagtaga ctcaaactca gcaagccaaa gtgcctcaaa ctcaacaagt acaagcacga
     4501 gcgaatccga ttcacaaagc acatcatcat atacaagtca gtcaacaagc caaagtgaat
     4561 ccacatcgac atcaacgtca ctaagcgatt caacaagtat atctaaaagt acgagtcaat
     4621 caggttcggt aagcacatca gcgtcattaa gtggttcaga gagtgaatct gattcacaaa
     4681 gtatctcaac aagtgcaagt gagtcaacat cagaaagtgc gtcaacatca ctcagtgact
     4741 caacaagtac aagtaactca ggatcagcaa gtacgtcaac atcgctcagt aactcagcaa
     4801 gcgcaagtga atccgatttg tcgtcaacat ctttaagtga ttcaacatct gcgtcaatgc
     4861 aaagcagtga atccgattca caaagcacat cagcatcatt aagtgattcg ctaagtacat
     4921 caacttcaaa ccgcatgtcg accattgcaa gtttatctac atcggtaagt acatcagagt
     4981 ctggctcaac atcagaaagt acaagtgaat ccgattcaac atcaacatca ttaagcgatt
     5041 cacaaagcac atcaagaagt acaagtgcat caggatcagc aagtacatca acatcaacaa
     5101 gtgactctcg tagtacatca gcttcaacta gtacttcgat gcgtacaagt actagtgatt
     5161 cacaaagtat gtcgctttcg acaagtacat caacaagtat gagtgattca acgtcattat
     5221 ctgatagtgt tagtgattca acatcagact caacaagtgc gagtacatct ggttcgatga
     5281 gtgtgtctat atcgttaagt gattcgacaa gtacatcaac atcggctagt gaagtaatga
     5341 gcgcaagcat atctgattca caaagtatgt cagaatctgt aaatgattca gaaagtgtaa
     5401 gtgaatctaa ttctgaaagt gactctaaat cgatgagtgg ctcaacaagt gtcagtgatt
     5461 ctggctcatt gagcgtctca acgtcattaa gaaaatcaga aagtgtaagc gagtcaagtt
     5521 cattgagttg ctcacaatcg atgagcgatt cagtaagcac aagcgattcg tcatcattaa
     5581 gtgtatcgac gtcactaaga agttcagaaa gcgtgagtga atctgattca ttaagtgatt
     5641 caaaatcaac aagtggttcg acttcaacaa gtacatctgg ttcattgagt acctcaacat
     5701 cattaagtgg ttcagaaagc gtaagcgagt ctacctcgct aagtgattca atatcaatga
     5761 gtgattctac tagtacaagt gactccgact cattaagtgg atcaatatct ttaagtggtt
     5821 ccacaagtct tagcacttcg gattcattaa gtgattcaaa atcattgagt agctcgcaaa
     5881 gtatgagtgg atcagaatca acgtcaacaa gtgtgagcga ttcgcagtca agctcaacaa
     5941 gtaatagtca atttgactct atgagcatca gtgcatcaga aagcgactca atgtctacaa
     6001 gtgattcgtc tagcatcagt ggatcaaatt caacgagtac atcactttca acatctgact
     6061 caatgagcgg aagcgtatca gtttcaacat cgacaagttt aagtgactca atatcaggtt
     6121 caacaagtgt aagtgactcg agctcaacaa gcacatctac atcattaagt gattcaatgt
     6181 cacaaagcca gtcaacaagt acaagtgcat ctggttcctt aagtacatcg atatcaacat
     6241 caatgtcaat gagtgctagt acatcgtcat cacaaagcac atcggtgtcg acatcattat
     6301 caacatcaga cagtatcagt gattctactt caataagtat cagtggttca caaagtacag
     6361 tagaatcaga atctacaagt gattcaactt ctatcagtga ctcagaatca ttgagtacat
     6421 cagattcaga ctcgacatcg acaagtacat cggactcaac aagtggttca acttcaacaa
     6481 gcatatctga atcattaagt acgtctggtt caggttcaac gagcgtatct gactcaacat
     6541 caatgagtga atctaattca tcgagtgttt caatgtcaca agacaaatcc gactcaacat
     6601 caattagtga ctcagaatca gtgtcaacaa gcacatcaac gtcattgagc acatccgatt
     6661 cgacaagcac atccgaatca ctgagtacat ctatgtctgg ttcacaaagc atttctgact
     6721 caacatcaac aagtatgtcc ggctcaacaa gtacatccga atctaactca atgcatccgt
     6781 cagactcaat gagtatgcat catactcaca gcacgagcac atctcgctta tcaagtgaag
     6841 caacaacgag cacgagtgaa tctcagtcta cattaagtgc aacatctgaa gtgactaaac
     6901 ataatggcac accagcacaa agtgaaaaaa gattgccaga tacaggtgac tcaataaaac
     6961 aaaatggatt actaggtggc gttatgacat tattagttgg tttaggttta atgaagagaa
     7021 agaaaaagaa agatgaaaat gatcaagatg attctcaagc ataaattgaa tttatagctg
     7081 gctaaaatta aaaagactga ggcaccaatc actagaaaaa tcgatatagg attttaataa
     7141 ttagtgatga acccttagtc tttttaatgt ttttgatata aaagaggtgc aacatgttaa
     7201 aacttttaca acaatacgaa tataaaatta tatataaacg tatgctatac acatgtttta
     7261 tcttgtttat ttatatatta ggcacaaaca tttccatcgt ttcctataac gatatgcaag
     7321 taaaacatga gtcatttttt aaaatagcta tttctaatat gggtggagac gttaatactt
     7381 taaacatttt cactttagga ttaggtccgt ggttaacatc aatgattatc ttgatgctga
     7441 tttcttatcg gaatatggat aaatacatga aacaaacaag tcttgagaag cattataaag
     7501 agcgcatctt aacattaatt ttaagtgtta ttcaaagtta ttttgtgatt catgaatatg
     7561 tttcgaaaga acgtgttcat caagacaata tttacttaac aatattgatt ttggttactg
     7621 gaacgatgtt attagtttgg ttagctgaca aaaatagtcg ctacggcatc gcaggaccaa
     7681 tgccgattgt aatggttagt attattaaat caatgatgca tcaaaaaatg gaatatatag
     7741 atgcaagtca tattgtgatt gcgttgttaa tcatccttgt gataatcaca ttgtttatct
     7801 tattatttat tgaattagta gaagtgagaa taccatatat cgatttaatg aacgtttccg
     7861 caacaaatat gaaatcttat ttatcttgga aagttaaccc tgcaggcagt attactttaa
     7921 tgatgagtat ttcagctttt gttttcttaa aaagtggcat tcattttatt ttatctatgt
     7981 ttaataaaag catatcagat gacatgccaa tgctgacatt tgatagtcca gtaggtattt
     8041 cagtatattt agtgattcaa atgttattgg gttatttttt atcgagattt ttaatcaata
     8101 ctaaacaaaa atccaaagat ttcttaaaga gtggcaatta tttttcagga gttaaacctg
     8161 gtaaggatac agaacgttat ttaaattatc aagcacgacg cgtatgttgg tttggattgg
     8221 cattagttac agtcattatt ggtataccgc tttatttcac attgtttgta ccgcatttat
     8281 ctactgaaat ttatttctca gtacaactga ttgtattagt ttacatcagt attaatattg
     8341 cagaaacaat tcgtacatat ttatattttg ataaatataa gccattttta aaccagtatt
     8401 ggtaaggagg taattatgaa atactttatt ccagcttggt acgatgacca acgatggtgg
     8461 caagacacga ctgtgccgta ttatcaacta caaaataaga cggaatttga cgatatgatt
     8521 agtttaatgg gaatgcacct tgaaaatgac ttagattatc aactgattgt tctcaatcat
     8581 gcaccaaatt taagaacatt tttacatcga tatgacttat atgagacaaa gtattcgtct
     8641 gtgtttgatg aaattcaagg attcagtcac catgcgccac aagcgattaa ttatcatcac
     8701 ttaaaatggc cggatgatgt tgagtttgtg tacacaccgt atttattaaa atgtgtgacg
     8761 agtgaacaga cctatacaaa tatttatttt agtcaagaag ggtattcaat ttggtttgaa
     8821 gaatttgaaa gagatcagtt acaacggcgt tatatttttg atgacagagg ttatttatca
     8881 gcgatacgtt attttgatga tcagggagag gcttcttacc aagaatattt aacgattaat
     8941 ggagattgtg tacttcatga agatttgaaa aatggcagag tcactgtttc aaaaagatat
     9001 caacatcact atcaacaaac agaatataac aatatggctc aactaattga agaaaaattt
     9061 caagcaatga ttgcacaaca aatacatgaa gatgatcatg tgattgtggc ttcagatgct
     9121 aggcacaatc gacaaatagc caatcatatt ccagcgaaat cattaagtta ttcatttttt
     9181 aaaaatagaa atgaaactgt gtcagatgag gaatatcaat ctatcgtaaa gaatgcccat
     9241 ttaattgttg atagtgtgca actagaacgt gatttaatta gtcatcaaga gaagtatcag
     9301 cgggagaata caatgattcg aatcacacca tttgaaacga gacaatcacc taatataagt
     9361 agtcaattga tggaaacatt tataggtgta tggatagatg gtatgagtga cgctgatttg
     9421 caacaaatga tgcaacgact tgtggattat atcgcacagg aagattatta ccgtttaatt
     9481 ttattatcgc gccatcaaaa tgacataccg atgtggcttc gtgaatgtat tacgtcggta
     9541 aatgaggaat accaagctaa acagaatgcg gatgttaatg tttcagcatt aatgacacct
     9601 gaagatcaag atgacatcat tgctgttaag acgatacatg ctgaacatga tgttgtagaa
     9661 gcattgcgga cgttgcgact tgtgatagat atgtcaaaag aacctgactt gtatttacaa
     9721 attagtgcaa ttagcgctgg gattccacaa attaatggtc aacaaacaga ttacgtctct
     9781 gattatgaca atggccgtat tataaataca gttgatgaat tagatgatgc gttaaattat
     9841 tatttatttt atttgaaaaa ttggaattat gcgtacgcct attctttaaa attaatagat
     9901 gcatatgctt ctaagaatat tattaatcag ctcgatgagt taatagaagg tgaaaatgat
     9961 gccacgtaaa tttagagttt tgcaaattgg aggagacgat ttagaaccta tttttcaaca
    10021 caaaaaaggt gtgagttggg attacttcga tattggattg tttgaatttg atagtggtta
    10081 tgtagaggct attgaagcga ttgttgaagc agaagggcgc tttgatttta tctatattca
    10141 agcaccatac tcggagacat taacgaattt attacaaatg ataagcgaac catacaatac
    10201 gtatgttgat gaatcatttt ggtcagttga atatgaacaa gacgaaaatg tccaaaaata
    10261 cgttgttcaa ccattacatt accggaatat tgaagaacgt aataataaat tagaggcagt
    10321 tagcttctca ggacagtatg gagataaggt ttctcctaag ttagcacttg tgcatccgaa
    10381 ttttaaagga gatgtcgttt accaaggtaa ttcagagctc acattgagtg gagaatttgg
    10441 gaaagaattt aaacctatcg catcttggca aaataatctc gtttacgata aagataaagt
    10501 cattcaaata tggccagaat ttgatattga tggtgcggtc gagttgcaat atacatttag
    10561 attgattcag actggcgctg atggtgcatt aattgaacaa atcgtattga ctgatgatat
    10621 gttagacagt cctttagaga tacctgcgaa accatttgat gcttatataa gtgtaactgt
    10681 taaggcgcgt gggaacggga cggtacattt aggacctata cacaaacgat ggtctagatt
    10741 agacatgggt caatttttac ttggtggtag tcgtttcgta gatagtcaac gacaggaatt
    10801 tatttattat ttccaccctg gtgatatgaa accgccacta aacgtatatt ttagtggtta
    10861 tcgaacagcg gaaggtttcg aaggatatta tatgatgaaa cgtatgaatg caccgttttt
    10921 acttatcggc gatcctcgtg ttgaaggtgg tagtttttat atcggttcat ctgaatatga
    10981 acaaggtatt atcaatgtta ttgacgagac attagaaaag ctgaatttta aatcacatga
    11041 attaatatta tctggtttat ctatgggttc atttggtgcc ttgtattatg gtgctcagtt
    11101 aaatcctcag gcaattattg ttggtaaacc acttgttaat attggaacga ttgctgaaca
    11161 tatgcgatta cttagaccag aagagttcgg tacagcatta gacgtcctgg tgtctaatga
    11221 aggtgataca tctcaagcgt cgatacaggc attaaatcaa aaattttggc aaacatttca
    11281 aaagaaatca ttgtcacaaa ctgtttttgc catagcatat atgcaacatg atgattatga
    11341 tccacatgca tttcaagagc tattacctgt attgacagca caccaagcaa gagtgatgaa
    11401 tcgcagtatt cctgggcgac ataatgatga ttcacctacc attgcaagtt ggtttgttaa
    11461 tttttacaac attattttgg aagacaagtt tgggagagtt caacatgctg aaaaacaaaa
    11521 catttaaagt tacttggaat tatatttcac aaactacatt tatgtatggc tctaaagtat
    11581 cattttcaaa tggtgaagtg acttttataa atcctttgat gccctctgga ttaccgattc
    11641 atgagtggtt gatgttaaag caattttcaa aatataaaag tgcgccaagt ttaccaattt
    11701 tgcgtagagg acagcattat aaattgcatt ttgattttga tgcaacgcct gctggttcag
    11761 tgtattttat catcattttt tataataaaa atggcacaaa actaagtact gagattgtaa
    11821 agtcaaatag tattacgatt caatatcctg atgaagccta tgcttataaa ataaaaatga
    11881 tgaatgcagc atcgacgagt cttatattta gatgtttaac aatcacagag atgacgcatc
    11941 aatatgattt agagtataaa agcatgcggg tgacgaagat aggcgatgac caatatggaa
    12001 atgatatgat caatgtcatt attgcagaac catctgacac ctatccaact atttcgaatg
    12061 attttttgaa actgtttggt catgtttggc tcgttgaacg ttggatggac gacaatatcg
    12121 agggaaatat taagcaacta aagaatgatt tgcaaagtca agatacccta aaggctatta
    12181 atttgattag ttatgggtca aagtcaaatg tttttgcaac ttatgtcgct cagcatttgg
    12241 gttgcaaagt gtatcgtaca agccatgaag atgatgattt aaaaggatgg cttacagaac
    12301 atgtcccagg aaatcatgaa ctaaaagata caaatgtcga ggtttatttt aaagaagagc
    12361 aagacaaaca tctaaattat atgtcacgtt taatgaatcc agtacgattt ttagattact
    12421 tagatgtatc gaaactgaat ggtggggaag taaatgaaac ataaactaga tgttacgata
    12481 aacgaattaa gactaaagtc gattagaaaa atagtgaaac gcatcaatac atggagcgat
    12541 gaagttaaat catattctga tgatgcgtta aaacaaaaga caatagaatt taaagaacgt
    12601 ttagcatcag gagttgatac actagataca ttgttacctg aagcttatgc agtggcacgt
    12661 gaagcgagct ggagagtatt aggtatgtat cctaaagaag ttcagttaat cggagcgatt
    12721 gtattgcatg aaggtaacat tgcggagatg caaacaggcg aaggtaaaac attaacggca
    12781 acgatgcctt tatatttaaa tgctctttcg ggaaaaggaa catatttaat tacaacgaat
    12841 gattatttag cgaagcgaga ttttgaagag atgcaaccat tatatgaatg gttaggttta
    12901 actgcttcat taggttttgt tgatattgtg gattatgagt atcaaaaagg ggaaaaacgt
    12961 aatatatatg aacatgatat tatatataca actaatggac gtctcggttt tgattattta
    13021 attgataatt tggctgatag tgcggaaggg aaatttttac cacaattaaa ttacggtatt
    13081 attgatgaag tggattcaat cattttagat gctgctcaaa caccattagt tatttcgggt
    13141 gcaccaagat tacaatcaaa tctatttcat attgtgaaag agtttgtaga tacattgatt
    13201 gaagacgtgc attttaaaat gaagaagacc aaaaaagaaa tatggctgtt aaatcaaggt
    13261 attgaagcgg cacaatcata ctttaatgtt gaagatttat atagcgaaca agcgatggtc
    13321 ctagtgcgta atattaattt agcactgcgc gcacaatatt tgtttgaatc taatgtcgat
    13381 tactttgtat ataatggtga tattgtttta attgaccgta ttacaggtcg tatgttaccg
    13441 ggaactaagt tgcaagctgg acttcaccaa gctattgaag cgaaagaagg tatggaggtt
    13501 tcaacagata aaagtgttat ggcaacaatt accttccaga atttatttaa actttttgaa
    13561 tcattttcag gtatgacagc tacaggaaaa ttaggcgaat cagagttctt tgatttgtat
    13621 tcaaaaatag tcgtacaagt accaactgat aaagcgattc aacgtatcga tgaaccagat
    13681 aaagtgtttc gttcagttga tgagaaaaac atcgcgatga ttcatgatat agttgaactt
    13741 catgaaacgg ggcgaccggt tttactcata acgagaactg ctgaagcggc tgaatacttt
    13801 tcgaaagtat tattccaaat ggatattcct aataatttac tcattgcgca aaatgttgca
    13861 aaagaagcgc agatgatagc tgaagcaggg caaattggtt ccatgactgt tgcgactagt
    13921 atggcaggtc gaggcacaga tattaaactt ggtgaaggtg tcgaagcatt aggtggatta
    13981 gctgttatta ttcatgaaca tatggaaaat agccgtgtag acaggcaatt acgtggtcgt
    14041 tctggtagac aaggggatcc gggatcatct tgtatatata tttcactaga tgattattta
    14101 gttaagcgat ggagcgatag taatttagcg gaaaataatc aattatattc attagatgca
    14161 caacgattat cgcaaagtaa tttgtttaat cgcaaagtta agcaaattgt agttaaagcg
    14221 cagcgtatct cggaagaaca aggggttaaa gctcgtgaaa tggctaatga atttgaaaaa
    14281 agcattagta tacagcgaga tcttgtatac gaggaacgca atcgagtttt agaaatagat
    14341 gatgctgaga atcaagattt taaagcgtta gctaaagatg tatttgaaat gtttgtaaat
    14401 gaggaaaagg tgctaacaaa atcgcgtgtt gtcgagtata tttatcaaaa tttaagtttc
    14461 caatttaata aagatgtggc ttgtgttaat tttaaagata agcaagcagt agtgacattt
    14521 ttattagagc aatttgaaaa gcaattagct ttgaatcgta aaaacatgca aagtgcatat
    14581 tattataata ttttcgttca aaaagtcttt ttgaaagcaa ttgattcatg ttggttagaa
    14641 caagtcgact atttacaaca attaaaagca agcgttaatc aacgacaaaa tgggcaacgt
    14701 aacgctattt ttgaatacca tagagttgcc ttagattcat ttgaagttat gacacgaaat
    14761 atcaaaaaaa gaatggtgaa aaatatttgt caaagcatga ttacatttga taaagagggt
    14821 atgccagtta tacattttcc ataaatgaaa aggggtagcg catgacaata tataatataa
    14881 actttggtat aggctgggcg agtagcggtg ttgaatatgc acaagcttac cgtgctaaat
    14941 tattgagacg tacaaatcaa gacactaaat ttgtgttttt agattttata cagtcagaga
    15001 acatacagac attaacgcaa aacattgggt ttaaagatca agaagttatt tggttgtatc
    15061 agtattttag tgatatttct ttagcaccaa caacgtacac gttagatgac attgaacgtg
    15121 agcttggtca tgacattagt tttagagaaa gaaatggtaa gatagtccgc ctatatttta
    15181 atgggaaatc atcatttgta acatgttatc tgcaaaatga acaaaaagat attgtggatc
    15241 gtgcagaatt tgtaattaat agtatgttag tacgtaaaga tttttatagt tatacgcgca
    15301 tattttctga gtattatgca ccagctgata ataaagcgaa attatatatg cgacaatttt
    15361 ataatcaaga tggttccatc gcttataacg aatatattga tggagacagc tctatatatg
    15421 tgtttgaaga tgcggtgttg tataacaaaa cggaatttat tgcatacttt ttacaacgat
    15481 tgaatttaac ccgagacgat attgtcatat tagaccgcgc ttctgatatt ggtcaggctg
    15541 ttttgcagca taaaggtgat agtaaagttg gtgttgtgat tcatgctgat cattatagta
    15601 ataatatgat gagtgagcag catatactat ggaacaatta ttatgaatat caattttcaa
    15661 aagctaaata cattgatttc tttattacag caacagatat tcaaaatcat atggtttgtc
    15721 gacaatttga gcaatatcaa ggttatcgcc cacgtgttta tactattcct gtgggaagca
    15781 ttgatgcatt atcatatcca acattatcaa gaaagccata tgcgatgatt agtgcatctc
    15841 gtttagcgaa tgagaaacat attgattggc ttgtcaaagc ggttatcgtg gctaaacgac
    15901 aagtgccaga attaacattt gatatttatg gagaaggtag cgagaagacg cgactgcgta
    15961 aaattattga tacacaccgc gcacaagatt atattcgttt actagggcat gttaaacttg
    16021 atgaaattta taatgattat gagttatttc tttctgcatc aactagcgaa ggttttgggt
    16081 taacactaat ggaagcggta ggctcaggac taggtatgat tggattaaat gtgaattatg
    16141 gtaacccaac ctttattcga gatggggaaa atggctactt ggtacctttt gataccgacg
    16201 aagatagtgt ggatgatgtt atcgcaaaac tagctcatgc cattgtaatg tattttaaca
    16261 atggtcccca gacaccacat gacatctctt atgaagtggc acagcaattt atgactcaag
    16321 atattatttt aaaatgggaa acattagttc aagaggtgct acatgattaa attatttgat
    16381 tactttaacg atcattcacg aaagttatat gagtctttta aagccagtaa gttggaagaa
    16441 gatttaacaa ttgtactcaa tgacaatggc tttttaccgg acgatgttat atcaccgtat
    16501 caattttttg cagataatca taatacagaa aatatgaaac cacgattttt taatcaggtt
    16561 actgtaccag ctttttggga aattaaaggt aacaacaatt cggctacaat taatgatatg
    16621 ggacgtctac gaggtaaaat attttatcaa agtggagagc gccctagaat tgttagtcgt
    16681 gtcgaatggt ttgatgacca acaacgtgtc cgatttgtcg attattattc taagaatggt
    16741 attaaatttg cacaaactgt ttacgattta aatcgtaaag cgatcttgaa gaaatatatg
    16801 acagcagaag gtaaagaagt gatttatgaa aattttgtaa catctgatgt catattggat
    16861 tggcaaggga agtcttactt tttcccttca aagctcgcat tcgcattatt ttttatcaag
    16921 caacttgaaa ttacggaaca tcatttcgtt attaactcac tagccctacc attttcagtg
    16981 ttatataatt tgccatcaaa aggtagtgat gttttggtat ggcaagaaca ctgtaatggt
    17041 aatgtcccgg gaaatatgca attgatgtgt aaaggtgata tgaaacgaca ttgtaatatt
    17101 attattccag ataaaaacga atatgaaacg atgctcaata tagcagacgc aaaggttcaa
    17161 tctcgtattt tacaaggcgg atatctatat aactatcgta gtcgtaatag atatactaaa
    17221 gaaattgtaa tattaactaa ctcggatcaa cttcgtaaca tcaaggtatt ggttgaaact
    17281 ttaccagatt ttaagtttca catcgctgct attacagaga tgtctgataa gctaatgcaa
    17341 cttgatcaat atgctaatgt gcatttatac ccatctatta atattgacag ggtaaatgaa
    17401 ctataccagt tatgtgatat ttatcttgat atcaatgagg gtaatgaaat tttaaatgcg
    17461 gtggaacaag cgtttgacta tgagttgttg attttaggtt atcgtcaaac cgcacaccat
    17521 gctaaagtaa cattatcaga gcacttattt gagcataatg atgagataac aatggaaagt
    17581 aaagaccaat taatacaaat gttagaaagt ttgaaagatc aacaacagtt tcgagatgct
    17641 ttattagcac aaaaagcaca tgcacacgaa atctcgcgtg aacaattcga gcaagttttt
    17701 aaacaggcgc tcgaaagcta aaaggaaaaa gcagtctaaa ataaaacatc atttaatata
    17761 aaagatttga cgtaatatga tgttagcgac atggacaata tgatattacg tcatgcttgt
    17821 tttgtttact taaatataag ctaagtaata agtagataat tactaacaac aataactaga
    17881 tagataagtg taaatttctt gtaaacaggt atataatagt atgtaattct ttgaaggaag
    17941 taagtgagga aggagtatgt tgatggctaa atatcgaggg aaaccgtttc aattatatgt
    18001 aaagttatcg tgttcgacaa tgatggcgac aagtatcatt ttaacgaata tcttgccgta
    18061 cgatgcccaa gctgcatctg aaaaggatac tgaaattaca aaagagatat tatctaagca
    18121 agatttatta gacaaagttg acaaggcaat tcgtcaaatt gagcaattaa aacagttatc
    18181 ggcttcatct aaagaacatt ataaagcaca actaaatgaa gcgaaaacag catcgcaaat
    18241 agatgaaatc ataaaacgag ctaatgagtt ggatagcaaa gacaataaaa gttctcacac
    18301 tgaaatgaac ggtcaaagtg atatagacag taaattagat caattgctta aagatttaaa
    18361 tgaggtttct tcaaatgttg ataggggtca acaaagtggc gaggacgatc ttaatgcaat
    18421 gaaaaatgat atgtcacaaa cggctacaac aaaacatgga gaaaaagatg ataaaaatga
    18481 tgaagcaatg gtaaataagg cgttagaaga cctagaccat ttgaatcagc aaatacacaa
    18541 atcgaaagat gcatcgaaag atacatcgga agatccagca gtgtctacaa cagataataa
    18601 tcatgaagta gctaaaacgc caaataatga tggttctgga catgttgtgt taaataaatt
    18661 cctttcaaat gaagagaatc aaagccatag taatcgactc actgataaat tacaaggaag
    18721 cgataaaatt aatcatgcta tgattgaaaa attagctaaa agtaatgcct caacgcaaca
    18781 ttacacatat cataaactga atacgttaca atctttagat caacgtattg caaatacgca
    18841 acttcctaaa aatcaaaaat cagacttaat gagcgaagta aataagacga aagagcgtat
    18901 aaaaagtcaa cgaaatatta ttttggaaga acttgcacgt actgatgata aaaagtatgc
    18961 tacacaaagc attttagaaa gtatatttaa taaagacgag gcagttaaaa ttctaaaaga
    19021 tatacgtgtt gatggtaaaa cagatcaaca aattgcagat caaattactc gtcatattga
    19081 tcaattatct ctgacaacga gtgatgattt attaacgtca ttgattgatc aatcacaaga
    19141 taagtcgcta ttgatttctc aaattttaca aacgaaatta ggaaaagctg aagcagataa
    19201 attggctaaa gattggacga ataaaggatt atcaaatcgc caaatcgttg accaattgaa
    19261 gaaacatttt gcatcaactg gcgacacgtc ttcagatgat atattaaaag caattttgaa
    19321 taatgccaaa gataaaaaac aagcaattga aacgatttta gcaacacgta tagaaagaca
    19381 aaaggcaaaa ttactggcag atttaattac taaaatagaa acagatcaaa ataaaatttt
    19441 taatttagtt aaatcggcat tgaatggtaa agcggatgat ttattgaatt tacaaaagag
    19501 actcaatcaa acgaaaaaag atatagatta tattttatca ccaatagtaa atcgtccaag
    19561 tttactagat cgattgaata aaaatgggaa aacgacagat ttaaataagt tagcaaattt
    19621 aatgaatcaa ggatcagatt tattagacag tattccagat atacccacac caaagccaga
    19681 aaagacgtta acacttggta aaggtaatgg attgttaagt ggattattaa atgctgatgg
    19741 taatgtatct ttgcctaaag cgggggaaac gataaaagaa cattggttgc cgatatctgt
    19801 aattgttggt gcaatgggtg tactaatgat ttggttatca cgacgcaata agttgaaaaa
    19861 taaagcataa ttatattggg ggaagagcat ctatatattt ttttaagtat ataagacgtc
    19921 ttatttcccc ttaatttatt gtgaagtata tgcaaaatgc aatgaataga ttgtccatca
    19981 ttttaacgtt ataatgaatt taacgactta gaactacaca agtaaaggag aatgaagatg
    20041 tctcgaaaaa cggcgctatt agttttggat atgcaagaag gtatagcgag tagtgtacct
    20101 agaataaaaa atattattaa agcgaatcag agagcaattg aagcagcaag acaacatcga
    20161 ataccagtca ttttcatacg tttagtgtta gataagcatt ttaatgatgt ctcctcgagt
    20221 aataaagtgt tttcaacaat taaagctcaa ggatatgcga ttactgaagc agatgcatct
    20281 acacgaatac ttgaagattt agcaccacta gaagatgagc cgattatttc taagcgacgc
    20341 tttagcgcat ttacaggtag ttacttggaa gtttatttac gtgcaaatga tattaatcat
    20401 ttagtattaa cgggtgtctc tacaagtgga gctgtattga gcacggcatt agaaagtgta
    20461 gataaagact attatattac tgttttagaa gatgctgttg gtgatagatc agatgataaa
    20521 catgacttta ttattgaaca aattttatca cgctcatgtg acattgaatc cgtagagtca
    20581 tggaaaagta gtttatagtt aatataacgt caattaaagc tcggcagtaa tgtttgagaa
    20641 taagtacatt tgctcatatt tataaaatgt gtgagatggc aattgaaacg gatatgatga
    20701 ggaacatttg aacataaaat aatatattta tataaaacga cccgaggcgt tcgaactgaa
    20761 tgcctcgggt ttaattgaat aagaaatcag acttatgaac agaaatatgt ttaagtccga
    20821 actccttgtt tatacttata aattttacgg gtttaatata atacttattt acctgtaata
    20881 tatgataatt cttcagcggc agctgcgttg atagttctat gagaaatgat acctaatcct
    20941 ttaacattgg attctgaaat aacgatagaa ccatcactgt taactttttc aacaaatgct
    21001 acatgaccgt aatgttgatc tgcaccaaat tgtccagcct caaatacaac agcagcatga
    21061 cgttttggtg tatgacttac ttgataatca cggtattgag ctcgattatt ccaattatgt
    21121 gcatcaccta aatcacctga gatagatgta ccaaattgtt tcatacggtt atatacgtac
    21181 caagtacatt ggccatgtgg atatggcata ctatcagata cctcacggaa aggtttgaat
    21241 tcatctgatg aatcatcata atccttgata gaacgttcat atttatctaa atctggcatg
    21301 cgttcatcgt caaactgagt taattgatag tgtttaataa tactgtttaa tttcttagca
    21361 tagtttggat ctgtagcata tgttttagat aagtgtgatg ttgcatcttt ataagaatcg
    21421 gcttccgatt tccatgttgg tttataaatt gttcgattgc catcaatacc atttttaata
    21481 aggtcagagt aatcttttag tgattctttc gtgcttggat attttcggaa tccagcatta
    21541 atactataca attgattacc atcagcttct aatgtgttaa aaggaacaga attcccttca
    21601 aaagcacctt tgataccgaa taaattatgg tttggtgact tagctaaagc actacgacct
    21661 gagtcagatt ctaagattgc ttgggcaatc atgacagacg cataaatatc gttatcttga
    21721 ccaatgcgat gtgcatcttt agcaattgat ttgacaaatt gacgtgtatc ttttgagtca
    21781 acaacgttaa attgtccgct atcatcattg ttagatatac taggatctgt ttcgaataat
    21841 gatgttgcac gtgtatcctt ttgattaaca tcgttattga atgattgagc aggtttagat
    21901 ttatgtttca attcatcttg tgttggtaac tgtggattct ttgtattaga tttttcattt
    21961 ttgtcttttt tagattgaga tgcataatct ttttgtgctt tctttgcatc ttcactgtat
    22021 tgatccaaaa tagagtctaa agccgaatct gacattgatt gattatcttt cgatgaagat
    22081 ttttgatttg ctttatcgtc acttgctggt tgactatttg attgattagg ttgtgttggc
    22141 tttggcgaat ttggttgctt attagatgta cttggttttg tattgtttga tttaggtgct
    22201 ttttgattgt ctgctttatc ttgtttagat gattgcgtat cagtgtcatt tttgatgcta
    22261 ttgtcactgt ttttattcga atcatttgtt gacttttcgc cattacgagg ttgttcgtaa
    22321 tcagaaatat ccgaatttaa attgaataag ttttggatta aagttgttaa tgagtaatta
    22381 tcatcgtatt tatttttggt tagcaattgg tttatattgg tttgtggtaa attcttataa
    22441 ataaaatcaa tgatattgtt agagtctgaa gtgctgtcgt ctatagtttt aaattttttg
    22501 tcgttattgt cttggttact tgtattattt ttgtctgctt tatcaatatc tttacttgta
    22561 gtatccttag aagtttcatc gtcattagat ttttttgaat catgagatgt tgtcttagct
    22621 gtagtatctt tttgaggtgt atcagcataa gcggtaggtg aaactaaagt aggtaatacg
    22681 agcgtagttg atagcaaata aattaaaatt ttatttttag gcatatttcg tattctccct
    22741 tgaaaaatat aataattaag tgtgataata aactatgatt tgttataatt tatcgtatgc
    22801 tgaaaatagt tgataggtat caatcgacta aatatcttcc agtaaattga ttatactaat
    22861 tcacaacgca aaaataaatt aatttacaaa aaatatataa aaaatatgaa taattcctac
    22921 ataggagtgt gacaatgaag aacgcattta aattatttaa aatggatctg aagaaagtag
    22981 ctaagacgcc agctgtgtgg attatcttag caggcttagc tattttgcca tcgttctacg
    23041 cttggtttaa cttatgggca atgtgggatc catatggcaa cacgggacac atcaaggtcg
    23101 cagtcgttaa tgaagataaa ggcgacacaa tcagagggaa aaaagttaat gtcggtaata
    23161 cgatggttaa tacactcaag aaaaataaaa gttttgattg gcagtttgta agtagagaga
    23221 aagctgatca tgagataaaa atgggtaaat attttgcagg tatttacatc ccatctaagt
    23281 ttacacatga aattacaggg acactacgta agcagcctca aaaagcagat gtagaattta
    23341 aggtgaatca gaagattaac gctgttgcgt ctaagctaac agatactggt tcgtcagttg
    23401 tcgttgaaaa agcgaatgaa caatttaata aaacagtaac tcgagcatta ttagaagaag
    23461 ctaacaaagc aggtttaact attgaagaaa atgtgccgac aattaacaag ataaaaaatg
    23521 cggtatattc agcagataaa gctttaccta agattaatga ctttgcgaat aaaattgtat
    23581 atttgaataa ccaccaagcg gatttagata aatatgccaa tgattttaga aaactaggaa
    23641 attataaagg tgatatttta gatgctcaga aaaaattaaa cgaagtcaat ggtgctattc
    23701 cgcaacttaa tgaaaaggct aagttgatat tagctttaaa taattatatg ccgaaaattg
    23761 aaaaagcgtt aaattttgca gctgatgacg tgccagcgca gttccctaaa attaatcaag
    23821 gacttaacat tgcgagtcaa ggtattgatc aagctaatgg acagttaaat gatgccaaag
    23881 gcttcgtcac acaagttaga agtagagtcg gtgattatca agatgcaatt cgacgcgcgc
    23941 aagatttaaa tcgaagaaac cagcaacaga ttcctcaaaa tagcgcggcg aacaacgaaa
    24001 catcaaatag tgcacctgca gctggtaatg gtgtagcatc aacgccacca agtgcaccaa
    24061 gtggcgatac tgcaccaaat aataatgtta cgcaaaatac cgcaccaaat agtaataatg
    24121 cgcctgtatc gactacacca caaagtacaa gcgggaaaaa agatggtcaa agttttgtag
    24181 atataacaac aacacaagtc agcacagcta acgagaacac acaaaacatt acagataaag
    24241 atgttaaatc aatggaagcg gcattaacgg gctctttatt atcattatca aataatttag
    24301 atacccaagc gaaagccgca caaaaagata gtcaggcatt acgtaatatt tcgtatggga
    24361 ttttagcatc ggacaagcct tctgatttta gagagtcttt agataatgtt aagtccggtt
    24421 tagaatacac aacgcaatat aatcaacaat ttatcgatac attaaaagag attgagaaga
    24481 atgaaaatgt tgatttatca aaagaaattg ataaggtaaa agcagctaat aatcgaatta
    24541 atgaatcatt aaggttagtt aatcaattaa gcaatgcatt aaagaatggt agttcaggaa
    24601 ctgctgaagc tactaaatta ctagatcaac tttcaaaact agattcatca ttatcatcat
    24661 ttagagatta tgttaaaaaa gatcttaaca gctctttagt atcaatatca caacgtatta
    24721 tggatgaatt gaacaaaggg caaactgcat tatccaatgt tcagtctaaa ttaaatacaa
    24781 ttgatcaagt cattaacagt ggacaagcta ttttaaaaaa tggtaaaaca cgtattgatc
    24841 gcttacaaac agtattacca agtattgaac aacaatacat tagtgctgtt aaaaatgctc
    24901 aagcaaactt ctcgaaagtg aaaagtgatg tagctaaagc tgctaacttt gtgcgcaatg
    24961 acttaccaca gttagaacag cgattaacta atgcgacagc aagtgtgaat aaaaatttac
    25021 caacgttatt aaatggttat gatcaagcgg taggattact aaataaaaat cagccacaag
    25081 cgaaaaaggc tttatcagat ttagctgatt tttctcaaaa taaattgcct gatgttgaaa
    25141 aagatttgaa aaaagcgaat aaaattttca agaaattaga caaagatgat gcagtcgaca
    25201 aattaatcga cacacttaag aatgatttga aaaagcaagc gggtattatt gcaaatccta
    25261 ttaataagaa gactgttgat gttttcccag ttaaggatta tggttcaggt atgacaccat
    25321 tctatactgc actgtcagta tgggtaggtg cactcttgat ggtaagttta ttaacggttg
    25381 ataataaaca taagagtcta gagtcagtct taacgacaag acaagtgttc ttaggtaagg
    25441 caggattctt tataatgctt ggtatgttgc aagcactcat tgtatcggtt ggagatttgt
    25501 taatcctaaa agcaggagtt gagtcacctg tattatttgt acttataacg attttctgtt
    25561 cgattatttt caactcaatc gtatatacgt gcgtatcatt acttggtaac ccaggtaaag
    25621 ccattgcaat cgtattgctt gtattacaaa ttgcaggtgg tgggggaaca ttcccaattc
    25681 aaactacgcc acaatttttc caaaacattt cgccatactt accatttacg tatgcaattg
    25741 attcattacg tgaaacagta ggcggtattg ttccggaaat cctaattaca aaattaatta
    25801 tattaacgtt atttggtata ggattcttcg ttgtaggttt aattttaaaa cctgtaacag
    25861 atccattgat gaagcgcgta tctgaaaaag ttgaccaaag taacgttaca gaataaaaat
    25921 taaatccaca cattagggtt atagctcctt aatgtgtgga tttttatgtt tttagacaga
    25981 agagatagta atttctgtct tttatgggac ggttgttatc attgctatta tccaggatga
    26041 cttactatag cactaatatt accgacaaag tgaatatcct cgtcttccgt agttaaaata
    26101 aagctagaac ctttttggat gtcatagtgc ttatcgttta ctgttaaagt accagtacca
    26161 tcgataattg taactaagca ataagcatgt ggtttattga attttaaatc tccatgaata
    26221 tcccatttat atactgcaaa atattgatta tctacaaatt gagttacagt gtgtgtgtcg
    26281 atgtgagttg ttataggagt agtatttggt tcatgattgc ctaattcaat cacatcttta
    26341 ctttgctcta agtgcaaatc acgcaattga ccattttgat ctcgtctatc atagtcataa
    26401 atacggtatg tcgtatcgga ggattgttgt gtctctaaaa ttaaaatacc cgaaccaatg
    26461 gcatggacag tgccagcagg aacataataa aagtcaccgg gcttaacagg tatacgtttg
    26521 aaaagactgt caaattcatg attatcaatc atgtctatta acgtctgttt attatgtgca
    26581 tgtacgccat atataatttc agcacctggg gctgcatcta aaatatacca acattctgtt
    26641 ttacctagtt cgccttcgtg ttttaaagcg tagtcatcat ctgggtgaac ttgaacagat
    26701 aatttatcat tggcatctaa tactttagtt agcagaggga aactatctcg tgaatcatta
    26761 tcgaataatt cacgatgttg tgaccaaagt tgatctaggg tcatatcctt gtatggacca
    26821 ttgataattg tattaggacc atttggatgt gcagaaattg cccagcattc accagttgtt
    26881 tcattaggga tatcatagtt aaatgctttt aatgcatgac cgccccaaat tctgtcttta
    26941 aaaacgggtt gtaaaaataa tgccatagtt aaaactcctc tatattttca ttaataagtt
    27001 ataaatttct gtagtactgt ttgcattaat tagtgattgg cgtgtctcat cattcattaa
    27061 cgctttagat aagcgctgaa gtatttttaa atgtgtatcc tgactgttgt ttggtacggc
    27121 aattaagaat atcaattgag gtagactacc atctagactg tcccatttaa caccatgatt
    27181 atttttcata acagctacaa tcggttgttt tacaacatca gactttgcat gtggaatggc
    27241 cacgttcatg ccaatagctg tcgtagaccc catttcacgt tctagtattg cattttttaa
    27301 atgcgatgtg tgctctacat aacggcaaat tttaagttta tgaatcaaca tatcaattgc
    27361 ttcgtttcga gacatgtcgt gatcagtaat tatcatagtt tgttgatcaa aaacatgaga
    27421 aggtttattg agatgtgaat gtttcgcggt gttatctaca ttgtcaacct ctgtatcatg
    27481 ttgtgtaata tctgtatcat gaagttgcgt gtgttgcgct ggtgcatcta ctgctataac
    27541 tggtgtattg cgttttaata atagtacagt agtcattgtg acaagactac ctactatcac
    27601 tgcaaagata aaccataata catgatcaat accacctaat acagccacga ttggacctcc
    27661 atgtgcgact ctatcgccga caccaccaat ggctgcaatg actgatgcaa tcattgcacc
    27721 aatgatgttt gcaggtataa tgcgcaatgg atcttgggct gcgaaaggaa tagcaccttc
    27781 agtaatacca aatagtccca tagtgaagga agccttaccc atttctcttt cggaatgatt
    27841 gaatttatac ttttgaacaa acgttgctaa acctaaaccg attggtggtg tacatacagc
    27901 aactgcgacc atacccataa cggcgtaatt accttcagca ataagtgctg agccaaataa
    27961 aaatgctacc ttgtttactg gaccgcccat atcgaaggca atcatcgcac ctataatcat
    28021 cgcaagtata ataatattag caccttgcat actttttaac caggttgtta atgcctcaaa
    28081 aatattagaa attggtgcac cgattaaaaa tataaatatc aatcctacaa cgaccgatga
    28141 aataatggga ataataatga taggcataat tggtgccatt gcttttggaa ctttaatatc
    28201 tttaatccac tttgcgatat aacctgctaa gaaaccagca acaataccac ctaaaaatcc
    28261 tgcgcctgca tcactgccat aaaaactacc gtcagcagcg atagcgccgc caatcatacc
    28321 aggaacaaga ccgggcttgt cagcgatact aacagcgata tatccagcta gtattggaac
    28381 cataaattta aaggctaaac taccaatgtt ttcaatggat ttccaaaatg aatcatctgg
    28441 gatgactaat ccttttgatg tcgtttcacc gcctagagtc agcgcgatgg cgataaggag
    28501 tccaccaact acgataaaag gaaccataaa cgatacaccg ttcattaaat gttgatacac
    28561 catttgaata ccacttttag acttaccgcg atctttcgaa tgataatttg tttcagattg
    28621 ataaataggc gcatcttgat taatgatacg ttgaattaga cctctcggat tatgaatccc
    28681 ttcgcgaaca ttttcattaa tcaaccgttt accaacaaat cgggacagat caacttgttt
    28741 atcagctgca attatgacac cgtcagcttc ttcgatgtct tgcgtagtta aaacattttc
    28801 agcaccaaca ccgccctgtg tctctacttt aatatccaca cccatttctt ttgctacctg
    28861 ctcaagcttt tcttgagcca tatatgtatg tgcaatgcca tttgggcatg aggtaatagc
    28921 tacaattttc ataaaatcat ctccttttct atattgtaag cgtattctcg atactaaaaa
    28981 aaagaataat taccgttact agtggcaatt attcttgtaa gtattcaaat aactgttgct
    29041 ttaaactatg atcatctaaa ctacataaat ggttcactga atcatcatcc aagttagcaa
    29101 ttaattgcat catttgtttt gtaaaagctt tgtctttatg cgaaatcgct aagaaaaaga
    29161 caagtttgac atcgtgttgt cgccaaggaa aaacatcttt tgtgcgaaaa ataagcacat
    29221 gtgattgtaa aactttttca ggatctccat gaggaatcgc cataaaatta cctatgtatg
    29281 tagaagatga tttctcacgc tctaaagctg attcgatata tccttctaca atcgcatgat
    29341 gtgcttgtaa tattttttga gcttcttcaa aaatttgcac agtatgccgt gatttttgtt
    29401 cagtatttac gacaaggaaa ttgacagtgt ccatatgatg atgtgcttga accggatttt
    29461 gcttttgctt cacaacgtgt ctgattttgt gacgatcatc ttcagaaaat aatggtgcaa
    29521 ccttgatagt cgtcaggtgc ttaggaagta tgtttagcgt ttgtttagga atatcatggg
    29581 tcgttattaa taaatctaca ttgtcaaagt gatagtgtgt tatattttct agtttaatcg
    29641 tatttatcac tgacaactct tcggataagt tatttatttt agtttctaaa aaattcgaca
    29701 cacctagacc ataataacaa gcaatgacta catttaattg tgttttggta cgacgctcga
    29761 tggcagcttg aaaatgaatt gttaaaaatg caatttcatc ttcgctcatc tctatatcag
    29821 tatcaattgc taatttatca atcgcttcaa aaagtgtgtt aaacacaaag ggatagagtt
    29881 ttttaatctc tataactaaa ggattgttta aataaatgtt ttgagtgata cgtaaatatg
    29941 ctttactaaa atgattatat aaattttgtt gtaaaatcga atcttcattg aaaggtacat
    30001 gaatacgttg ctgcatcaat tcgattaagc gatcaatata actttgtata aatatacgtt
    30061 ctatgccaat atcgagttta ttaaaatgat aagcaataaa gaatgaaaac atattgatta
    30121 ctttttcgtt caagtcataa cctaatcttt cgttgatttg cttaatgcaa gattgagata
    30181 tcaattttga atcgtggttt aacgtgtcta gctcacttaa agattgctta cgtttaatga
    30241 ttaaaatgag atgaattaaa agctgttgta tttgaatatc agttgtttca atactatgtt
    30301 gttgaagtgt ctcttgtata atatgcgaaa tcatcctttg gtgtgaatca ggtaattcat
    30361 ttaaaattag gtcttcaaca tgtacatgcc ctgatgataa ttgatttaaa tggatgatgg
    30421 cattagtgat atcattatct gttccatcga ccacaatacc ttgttttttc ttgattgtta
    30481 aagcgatatt aaatttttga caccattgtt gaattcgaac gaaatagtcg ttgagcacag
    30541 ttttagataa tagataatct tgctctagtt gttgcaatgt ctgcgattta ttgtgcatta
    30601 gcagttgata cgccatagat aataatagtt tttctttata tgattgaata tgcgtgtaac
    30661 gttcagttat cgtttctaag gtatactgag atgtatttaa ttgatatcct ttagatttaa
    30721 tacttacgat gatatcatcc ataaaattgc tattgataac atggatatca ttgcgaactg
    30781 tgcggtttga aacattgaca tgtttagcaa tttcattaga actaatgtgc tttgatggat
    30841 ttttaataaa aaactggagt agttttaagt gtctatcaag catttaacat gtacctcctt
    30901 tctaaatttt tcgtgtaagc gtttttaagg gtgtattaat attattaaac atgagagctt
    30961 atacatacgt caatgacatt aaagcgaact tttatatgat tttaacagag tgcgaattat
    31021 gcaaataaag aacagcagta agatatttca aatagaaaaa tatctcactg ctgtttttct
    31081 gaatttatgc atctggtact tgtggacgta tcaggcaaat gattaatttt taggtgattg
    31141 tgcttgaggt gtttgtttag aaggcgtatt ggtattattt gatttattag caggtggtgt
    31201 gttctgttgt tgattatttt ggttgtttgt tgacgattga ctgccaccac cgtgagtatt
    31261 atttttattt gaatttgagt cttttggctg agctggttgc gtattcgatg gtactgttga
    31321 atggttgtta ggaacatttg acggtgaaga tggttgattg tcattagtaa cgttattgcc
    31381 attattttga tttttatctg ttttgtctgc actatcatct tgttgatcat tttcttcggt
    31441 ttctgtcttt ttatgcgtag atttattttc ttgttcttgg ttattacttg atgatgtaga
    31501 atgatttatt gcagcataaa ttgcgaaccc aagtgctccg attaatacga taattacgat
    31561 ggtaataaga agttttttca aatataacgc ctccatgtta atgaaaagta gttgatacgt
    31621 gaagctaaat aatttatttc aatattgcca taatcatttt aaaaaataaa tattagaaaa
    31681 gtaggcgata ttaatatttt cattgttttt tgtgtacttc aattagtata acaaaactgt
    31741 tatgtattta taaatataat cattagtatt tttatggctg aaaaagttat aataaaagtg
    31801 taaggaataa aatatttgta tggaaaagag agataattat gaataaaacc agtaaagttt
    31861 gtgtagcagc aacattagca ttgggcacac tgattggagt aacagttgtt gaaaattcgg
    31921 cgccaacaag taaacaggca caggc
//
LOCUS       DANJFZ010000021        31913 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.021, whole genome shotgun sequence.
ACCESSION   DANJFZ010000021 DANJFZ010000000
VERSION     DANJFZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.021"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..843)
                     /locus_tag="PWN45_000829"
     CDS             complement(<1..843)
                     /locus_tag="PWN45_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL0622612.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENI"
     gene            953..1822
                     /locus_tag="PWN45_000830"
     CDS             953..1822
                     /locus_tag="PWN45_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0622613.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            complement(1881..4490)
                     /gene="clpB"
                     /locus_tag="PWN45_000831"
     CDS             complement(1881..4490)
                     /gene="clpB"
                     /locus_tag="PWN45_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL0622614.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(4693..6507)
                     /locus_tag="PWN45_000832"
     CDS             complement(4693..6507)
                     /locus_tag="PWN45_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0622615.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            complement(7054..7362)
                     /locus_tag="PWN45_000833"
     CDS             complement(7054..7362)
                     /locus_tag="PWN45_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL0622616.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            7476..8300
                     /locus_tag="PWN45_000834"
     CDS             7476..8300
                     /locus_tag="PWN45_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0622617.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            8352..9668
                     /locus_tag="PWN45_000835"
     CDS             8352..9668
                     /locus_tag="PWN45_000835"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDL0622618.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(9839..10228)
                     /locus_tag="PWN45_000836"
     CDS             complement(9839..10228)
                     /locus_tag="PWN45_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDL0622619.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(10557..11459)
                     /locus_tag="PWN45_000837"
     CDS             complement(10557..11459)
                     /locus_tag="PWN45_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDL0622620.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(11625..15278)
                     /gene="addA"
                     /locus_tag="PWN45_000838"
     CDS             complement(11625..15278)
                     /gene="addA"
                     /locus_tag="PWN45_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL0622621.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(15279..18755)
                     /gene="addB"
                     /locus_tag="PWN45_000839"
     CDS             complement(15279..18755)
                     /gene="addB"
                     /locus_tag="PWN45_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDL0622622.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            complement(18915..19490)
                     /gene="lepB"
                     /locus_tag="PWN45_000840"
     CDS             complement(18915..19490)
                     /gene="lepB"
                     /locus_tag="PWN45_000840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0622623.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            complement(19506..20030)
                     /gene="lepB"
                     /locus_tag="PWN45_000841"
     CDS             complement(19506..20030)
                     /gene="lepB"
                     /locus_tag="PWN45_000841"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0622624.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            complement(20035..20610)
                     /locus_tag="PWN45_000842"
     CDS             complement(20035..20610)
                     /locus_tag="PWN45_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0622625.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            complement(20935..22266)
                     /locus_tag="PWN45_000843"
     CDS             complement(20935..22266)
                     /locus_tag="PWN45_000843"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL0622626.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            22617..23822
                     /locus_tag="PWN45_000844"
     CDS             22617..23822
                     /locus_tag="PWN45_000844"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL0622627.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            23812..25191
                     /gene="argH"
                     /locus_tag="PWN45_000845"
     CDS             23812..25191
                     /gene="argH"
                     /locus_tag="PWN45_000845"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL0622628.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            25433..26362
                     /locus_tag="PWN45_000846"
     CDS             25433..26362
                     /locus_tag="PWN45_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0622629.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(26865..28109)
                     /locus_tag="PWN45_000847"
     CDS             complement(26865..28109)
                     /locus_tag="PWN45_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDL0622630.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(28218..29408)
                     /locus_tag="PWN45_000848"
     CDS             complement(28218..29408)
                     /locus_tag="PWN45_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL0622631.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(29716..30843)
                     /locus_tag="PWN45_000849"
     CDS             complement(29716..30843)
                     /locus_tag="PWN45_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL0622632.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(31205..31582)
                     /locus_tag="PWN45_000850"
     CDS             complement(31205..31582)
                     /locus_tag="PWN45_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL0622633.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
BASE COUNT        10389 a         5760 c         4564 g        11200 t
ORIGIN      
        1 tatattttca ccaattatag gtttgttttc tgatatttga aaatcgagaa ttttagaaat
       61 attaatacta gttttatata cgtcgggtaa agtgaattcc gtactttcta tttgtttttt
      121 tgtcaaaata gtaattattt cctcaatagg agcattaccc gctctctcac caattctcaa
      181 aaatgtagtt tctatttgct ttgcaccatt taaaattgca gctaatgtat ttgcagtagc
      241 caaccctaga tcgttatgac aatgagcaga aaaaattatg ttagaatatt tttttacaaa
      301 gtaattaaaa atatctccgt attctaatgg tgtcgaacat cctacagtgt ccgcaaatgt
      361 aactgttcta acttgatatt ttgaaataat ttccatgtat tctttcaatt tttctctaga
      421 agtccttgtt ccatcttcaa aacaaatatc tactcctttt ttatctttct ttaatatatc
      481 caagcagtct ttgattttct gaatataata tttatttgaa aaattaagct tttcttttat
      541 atgcaagtca gatattggca atagtatttt taccactaaa ttatgaattt tcaatttgac
      601 tatttttttt atttcttttt cattaagcct agtcaatact actaattctt tttcaggact
      661 taaaatgtct ctgaattgat gaatattaaa ttcatcctcg atagttgtac acatgcctac
      721 ttcaacagaa tttatattta acttgttaat ttgtttcaat acttcttttt ttataattag
      781 actttttcga acattacttt gttgcatacc atcccttatt gtattatctt gaattcgaat
      841 catattactt caccccctcg ataacctctg atataatatt aatattattt ttatgcaata
      901 aaaattaaat attatctaat gaattattaa cttttttgaa agaggtaagt tcatgaattt
      961 agattggtac tatacatttg tcattttagc gaagacgtta aactatcgat tagctagtga
     1021 agaaattaat ctcactatac cttctataca taagcaaatt aaaaatttag aacagcatct
     1081 caatgtgaaa ctatttgaaa cttataaaaa tcaaattatc ttgactgaag atggtcatac
     1141 atttcttcca attgcgcaaa gttttattga acaatatgaa agtggcatca aacatatcca
     1201 acttaaaaaa acgatgtttc aatcgaaatt aaatgttgtc gtgtcttcat atatcgcgac
     1261 attcattatg cccaaatttt taaaatcttt ctttaacgag catcctttta tcgatgtttc
     1321 tcttcatgtc aaaaatgaaa atattgaaaa agatattaat aatcatacgt atgacatcgg
     1381 gattagcaga aatcaaccta aactaagaga agttcattct gaaaaagtat gcgaaggtaa
     1441 aattgtattg attgctccca ataaagagaa taatcatctt ctaactgagg catctttatt
     1501 tgaaaaatac aaaataataa gtgataacca tccagaatat tggtcatcac ttaaaaataa
     1561 tattttaaat atttatgaaa aagcacaatt tctatctatc aatgatgtgc atacttcaat
     1621 taaattaatc gaaatgaatc aaggtatatc attcctgcct ctttatataa caacaaatag
     1681 tgattacaat atttcggtta ttaacacgaa aattttacaa gcacctattt catttacata
     1741 tatatacagc aaaaaagaaa gcccagaaat attggtgttt attaaatcat ttaaaaagta
     1801 tattgccaat gaacaattat aataaatttc aaatctaaaa aaccaagaat gcgattaatc
     1861 atcacattct tggttcaatt ttattcatga attttttcaa cattaaacgt taagttattg
     1921 tctgaattta aattaacttt aatcgttgta ccttctggga atccctcttt aatcatcata
     1981 cgtgctaatg gtgtttcaat ttggcgttgt acaaaacgtt ttaatggtct tgcaccgtat
     2041 tgaggttcat aagcttcttg acctagccaa gctttagcat catcagaaac ttcaattgag
     2101 attcgttgtt ctaataatct tatatttaat tgcgttaaga ttttatctac aatcatactc
     2161 atgtcatcaa tagataatgg tttaaataat acgatatcat ccatacgatt caaaatttct
     2221 ggtttgaaat atgcatttaa acttgtcata acagcttttt ctgttgattc tgtaatttca
     2281 ccagtctctt ttacgttttc taataaaact tgagatccaa tattacttgt cataataata
     2341 atagtatttt taaaatcaac gctacgtcct ttagaatcag ttaaacggcc ttcatctaaa
     2401 atttgcaata atacattaaa gacgtcagta tgcgcttttt caacctcatc taataaaata
     2461 actgagtatg gattacgacg aaccgcttca gttaattgac ccccttcatc atgtccaata
     2521 tatcctggag gtgcccctat caatcttgat actgcatgtt tttccatata ttcactcata
     2581 tcaatacgaa tcatatgttt ttcagaatca aataatgatg cagctaatga tttagctaat
     2641 tcagttttac ctactccagt tggacctagg aataagaaac taccaatagg tctgtttgga
     2701 tctttaatac ctgctcttgc tctaactact gcatctgaaa ccaggtcaac cgctttatct
     2761 tgacctacaa cacgtttatg caagatgtca cttaagtgaa gtaatttttc acgttctgtt
     2821 tcaactaatt ttgaaactgg tatgcctgtc cattggctga caatatcgcc aatttcttcg
     2881 tctgttacaa cttcacgaat cattcgatct gtatcttcac cttgctcatc ttggaaatta
     2941 tcctctaatt ctctaagttc tttttccaat tgaggaattg ttccatattg tagttcagca
     3001 gctttttcta aattgttatt tgtttgtgca tcttccaacg cttgtctact ttcatctagt
     3061 tgcgcacgtt tttcttgtaa atttgctatt ttttcttttt ctgattctac acgagattga
     3121 agtgctgctt gtttctcttt ttcattggca agctcttctt gtagttcttg taatctctgt
     3181 ttgctcgcat tgtcagattc atttttaagt gcgctttctt caatttctaa ttgcatgaca
     3241 cgtctattaa cttgatccaa ttcagttgga tttgatccca tttccgtacg aattgttgca
     3301 catgcttggt caactaaatc aatcgcttta tctggtaaaa aacgatcagt gatgtaacga
     3361 tcagacaatt cagcggcagc aactaaggct ctatcttgaa tacgcacacc atgatacact
     3421 tcatatcgtt cttttaaacc acgtaaaatt gaaattgtat cttcaacatc aggctcacta
     3481 actgctactt tttggaaacg acgctctaat gccgaatctt tttcaatata ttctcgatat
     3541 tcatttaaag ttgttgcacc aatacaatgt aactctcctc gtgctaacat tggttttagc
     3601 atgttgcctg catccatggc accatctgtt ttaccagcac ctacaagcat atggatttca
     3661 tcaataaata atataattct accatcagac tctttaactt cttttaggac tgcttttaat
     3721 ctctcttcaa attcaccacg atatttagcg cccgctacta atgcgcttaa atctaactca
     3781 aaaacagttt tatctaataa tgattctggc acatctttct taactatacg ttgcgctaat
     3841 ccttcaacaa ttgcagtttt accaacacct ggttcaccaa tgagcacagg gttgttttta
     3901 gttttacgac ttaaaatacg aatcgtattt cgaatttctt catctcttcc tataacagga
     3961 tccattttac cttgtctaac ttcttctact aagtcgcggc catatttagc taatgcttcg
     4021 taattaactt ctggattttg tgatgtcacg tgatttcccc ctcttacttt tttaataatt
     4081 tctttgataa cttctacttt attatttata taatgttttg ttgtttgatc aatgtccatt
     4141 gccgaacgta aaatatgctc cattgaaata tattcatctt catattcttt catgtatgat
     4201 tcagccttag ttatcaattg gtttgcttgt tggctaatat attgaccata ttgtatattg
     4261 tcaccttcta cagatgcata cgtgtttagt ttgtcttcat aagctttgtt taattgatct
     4321 acctcaatat ttgctcgttc taaaatactt ttatataagc tttcactttc atttaaggca
     4381 gcgcttaaaa ttgcctcaat ttctatattt tgtaatttat gttgctgact cagttcaact
     4441 gcttgttgta aagcactttg aacagcatat gtcattttat ttatatccat ataatttcac
     4501 ctctaatttg tagagttggt caagttgcac ttttgacttt gactttcttt gaccttaatt
     4561 atattatagt cccaatataa tcatttatca actcttttac acttaaattt cctaatattt
     4621 gtaaatattt ttttgaataa aaaaatctag ctaatgttac gttacacatt aactagactt
     4681 attaaacata tgttatttct ttttaagtgc ttttaatatt tcatctttta aagctaggac
     4741 tcctttgtac tctaaatgta caccgtctgg tgcaaaatat tcactatgtc cttgtgatcg
     4801 cttataccaa tcaattaatg tgacattgga ctttcgtttc gccgcgtcag ctaataatcg
     4861 atttacattt gcctcataaa ttcttggaac acgtgtatta actaaataaa tcttggcttt
     4921 tccaaattga ttaagtaaat cgtcgagctg tttgacagta aagtcgccat ttgtacctaa
     4981 ttctaatacg acttgatcag atgatttttt atattgtgaa taattcgctt taactaaagg
     5041 taaggtttga tacaattgac gccctacttt tccatcaatt ctagacttag gaactgatga
     5101 cttaaatgac tcaccgatat caaccattac tgaatcaccg attaaaagag gtttaatatt
     5161 attatatacg tcggaagatt cctttttctt ctcacttact aagccatcaa tgtgtatgtt
     5221 atcaattggt gctatccgca ttaaataatt atcttcaatt gtggtatcaa agctattcgc
     5281 tttttctcca ataatgtctt taccatattt attgaatgca cctactaaaa tcaacataaa
     5341 tggaattaac aaggttacta caattgccat tcttataaat tgtggtatat aagaaggtcg
     5401 ccaatttaaa gctttaatac cttcttttct aaatggagtt tctataaagc gatatgatag
     5461 ctctgcaaat ataattgtta aacttatatc tataaagtac acatatacag gtatctgtcc
     5521 gtctacatag taactatgta cgaaactaat tactgcaaaa tgccataaat ataaactata
     5581 agaccttttc ccgataaata ctaacactgg atttgaaaat atcttcgcta tccatgtaga
     5641 tggatgaacg acactagcaa taataaataa cgttaatatg gatattaaat agaaaccacc
     5701 atcatatatc caattcgtct catcattaat aatgaaaaat aataatataa gtactataaa
     5761 tgataaacta cctatgctat caataacata ttttacaact ttaggtggat catttttcaa
     5821 tttaaacggt ggccataaaa aagctaaaat aacacccagt aacaatgtct gtaatctagt
     5881 atctgtacca aaatacactc gtgaatgatc cccattaata ctatagataa acatcattaa
     5941 ccctaaagaa attattgata ctccccaaaa aataaatcct attttgtatc gctttttaat
     6001 tgttaacaat aatgtaacca aaataactgg gaaaaatatg taaaactgtt cttcaattgc
     6061 taaagaccat aaatgcttta atggcataaa tgaaaattgc tcaaaataat taacatcttt
     6121 tgctatatac caccagtttg atacataaaa tatcgcagca ataatatcat gtttaaccct
     6181 aatgatatta tctgatttta ataataaggt tgctgtccct acaaccatta ataaaactat
     6241 gactgctggt aataaacgtt ttaaacgacg tatccaaaag cttttcaatt taatgatacc
     6301 tgtgtcatca tactctttga gtaataagct tgtaattaaa taaccagaga tcacaaaaaa
     6361 tgtatccaca cctaaaaagc cacctgtcaa ccattgctta tttaagtggt aaataataat
     6421 tcctagaaca gcgattgccc tcaaaccatc gagccctgga atatatctca ttttcttata
     6481 ctttgtaaaa ccctttgttt tgttcatttt tgcattcttc ccttttaaaa ctgttcttct
     6541 tagatgctta attaaattta gttatgctgt ttaaaagaat attgaaatgc atatgtatat
     6601 tattgaatta cgacatcatc aaaatcatat tgactaaaat actgttaaat taaaaaaatt
     6661 accaatgatg attcttactt cgaaatccaa tttgtaatgc aactcggcaa cttaaaaact
     6721 atgaagtatt atgtattgta atataactgt aatataaatt aaatttatta taaaaatttt
     6781 caagaaaata ttcaactaga aaatgaattg tacactcttg gaagtgcaag tcactgtctt
     6841 aattcatatt ttttgaaaca agttagatat aaattttcaa aataaaatca gaaactagaa
     6901 cataaataag gctcccttca aaattttcat ttttcaatgt ctactttgaa gggagcttat
     6961 tcacaatgaa ttatactcta caatgttata ttgactgcgg gcccaaacac agagaatttc
     7021 gaaaagaaat tctacaggca atgcaagttt atgttagctc acaccaagtg caatcttagc
     7081 gtaacgtgac atcatatctt ttgtccaagg tggactccat acgatattca cttcagtatc
     7141 ctgaatttca ggaatctctg ctaatactgt tttaacttga tcaataattt gaggtcccat
     7201 tggacatccc attgatgtta aagtcatatc aactgtacat acgccttcat catcaacatt
     7261 cactttgtat actaaaccca aattaacgat atcaattcct aattcagggt caattaccat
     7321 ttctaatgca cctaagatac tatctttcaa tgcctcttcc atccatatca cctctttaat
     7381 gtcatattat tcataatata tcaaatatcc gacaaaacgc caataaaatg ctatgatgta
     7441 tctatatgaa ctaagcaact tatgaggaga gagatatgca accacattta atatgtctag
     7501 acttagacgg aacattatta aacgataaca aagaaatttc atcatatact aaacaagtat
     7561 taaatgaatt acaacaacgt ggacaccaaa ttatgattgc gactggcaga ccttatcgtg
     7621 caagtcaaat gtattatcat gaattaaatt taacgacacc aattgttaat tttaatggcg
     7681 cttacgtaca tcaccctaaa gataaaaact tcaaaacttg ccatgaaatt ttagatttag
     7741 gcatcgcaca aaacattatt caaggattac aacaatatca agtatcgaat attatagcag
     7801 aagtgaaaga ttatgttttc attaacaatc atgatccaag attatttgaa ggtttttcaa
     7861 tgggtaatcc aagaattcaa actggtaatt tacttgtcca cttgaaagaa tcccctacct
     7921 caattttaat tgaagccgaa gaaagtaaaa tacctgaaat caaaaatatg cttactcatt
     7981 tttatgccga tcatattgag catcgacgct ggggcgcacc attccctgtc attgaaattg
     8041 taaaacttgg tattaataaa gcaagaggca ttgagcaagt tagacaattt ttaaatattg
     8101 accgaaataa tattattgca ttcggtgatg aagataatga tattgaaatg attgagtacg
     8161 cgcgtcacgg tgttgctatg gaaaatggtt tgcaagaact taaagatgta gcgaacaata
     8221 ttacattcaa caataatgaa gatggcattg gtcgatattt gaatgatttc tttaatttaa
     8281 atattagata ttactgttaa tttataacta atcattttat aatattttaa aacaatagga
     8341 ggtaagttac gatgcccaaa atagtcgtag tcggagcagt cgctggcggt gcaacatgtg
     8401 ccagccaaat tcgacgttta gataaagaaa gtgacattat tatttttgaa aaagatcgtg
     8461 atatgagctt tgctaattgt gcattgcctt atgtcattgg cgaagttgtt gaagatagaa
     8521 gatatgcttt agcgtataca cctgaaaaat tttatgatag aaagcaaatt acagtaaaaa
     8581 cttatcatga agttattgca atcaatgatg aaagacaaac tgtatctgta ttaaatagaa
     8641 agacaaacga acaatttgaa gaatcttacg ataaactcat tttaagccct ggtgcaagtg
     8701 caaatagcct tggctttgaa agtgatatta catttacact tagaaattta gaagacactg
     8761 atgctatcga tcaattcatc aaagcaaatc aagttgataa agtattggtt gtaggtgcag
     8821 gttatgtttc attagaagtt cttgaaaatc tttatgaacg tggtttacac cctactttaa
     8881 ttcatcgatc tgataagata aataaattaa tggatgccga catgaatcaa cctatacttg
     8941 atgaattaga taagcgggag attccatacc gtttaaatga ggaaattaat gctatcaatg
     9001 gaaatgaaat tacatttaaa tcaggaaaag ttgaacatta cgatatgatt attgaaggtg
     9061 tcggtactca ccccaattca aaatttatcg aaagttcaaa tatcaaactt gatcgaaaag
     9121 gtttcatacc ggtaaacgat aaatttgaaa caaatgttcc aaacatttat gcaataggcg
     9181 atattgcaac atcacattat cgacatgtcg atctaccggc tagtgttcct ttagcttggg
     9241 gcgctcaccg tgcagcaagt attgttgccg aacaaattgc tggaaatgac actattgaat
     9301 tcaaaggctt cttaggcaac aatattgtga agttctttga ttatacattt gcgagtgtcg
     9361 gcgttaaacc aaacgaacta aagcaatttg actataaaat ggtagaagtc actcaaggtg
     9421 cacacgcgaa ttattaccca ggaaattccc ctttacactt aagagtatat tatgacactt
     9481 caaaccgtca gattttaaga gcagctgcag taggaaaaga aggtgcagat aaacgtattg
     9541 atgtactatc gatggcaatg atgaaccagc taactgtaga tgagttaact gagtttgaag
     9601 tggcttatgc accaccatat agccacccta aagatttaat caatatgatt ggttacaaag
     9661 ctaaataaat gaatcatgcg attcaatcta atcttaattg tctaggttag aattatgttg
     9721 gactggtact actatccagt ccatttttta tgtttaacat ttttagaatc aaaaaagaca
     9781 taaggtcttg gactaataat tgtccatgcc ttatgtcata tactatatgt cttatcaatt
     9841 agccaatacc gaataatttt gatataggcc ctaacggtag aatgacacct aataccattg
     9901 tgatgataat taatgcaatt gttatccaaa acattgtgtg actttgttca tgtctctttc
     9961 ttttagcaat cgacacttcc atcaatccaa ctactgcaac accacacagc attttcaatg
    10021 taagcaacat atgatttgcc ccgccattca taaatgactg aattaatatc caaaatcctg
    10081 aaattaacgt caacagcata aataagcgta aaatcatgtg caacggtttg aaaaatggtg
    10141 atctgccttg attttttgaa atgtttaagt atgtagcgat aaataaaata atcgctaata
    10201 cccaacttaa tatatgtaaa tgtaacatac tgattccccc cactttaatt atttatatta
    10261 ttaaattaaa gcttcttggg attaataccc acttgcttgt aatttaatca tgatttgatt
    10321 atacacgaat atatattcta ccacacttct atatttgaga ggaagaacat gacattttat
    10381 tccttattag aatattgtga atctgctgta aaataatcaa ctacttctta atatcaatat
    10441 ttcactttca tctcaaaatg gtaacattat aaataattta tctttaacac ctttttagaa
    10501 aagcaagaaa aaactaacca atctatataa agactggtta gctttttaaa tgataattat
    10561 ttagcgatat aagttgtcag cgttccaata ttatcaatag tcactttaac ttcatcacct
    10621 ggttgtaaaa atttaggtgg ttgcatacct gcaccaacgc ctgctggtgt accagttgca
    10681 ataatatctc ccggatgtag tgcaacatat tttgaaattt cttctattaa ttcatcaatt
    10741 ttaagaatca tttcgccagt gttaccatct tgtctaattt cattgttaac ttttgtaaca
    10801 atatttacat tttcaggtaa tggtagttcg tctttagtaa cgatataagg acccattggg
    10861 caaccgccag ttaaactttt tgataaaaat gcttgatctt gttcactttg tgctttgcga
    10921 tcagtgatat cgttaataat tgtatagccg taaacataat ctaaagctaa tgcttttgga
    10981 atcttttcac cagacttacc aataacaata cctaattcac cttcataatc taattgatca
    11041 gtaatatctt tatgatttgg aattgttgca ttatctcctg ttaaagatga cgctgctttt
    11101 gtaaatacat ataatttttc cacttcatga tttaattcgt tcgcatgatc tttgtaattt
    11161 ctaccaaaag caatcacatt attcggaggt gttactggtg gtaaaaattc aatgtcatta
    11221 aatgaaattt tatagtcttc agctttgccg ctatcttctg ctgctacaac tgctttacgt
    11281 acttgttctt gaaaatctaa agtatgattt tgttgtaaac cagctaacaa tgttttagga
    11341 tggaaatctc cttctgcaaa gtcagcaaat acttgtgtta aatcccatac agcatcttcg
    11401 cgttttactt taacgccata tgaagttttg tcattatact tgaatgataa gaatttcatt
    11461 cattctcaac tcctcatctt tatcttaatt cacattataa cttttttcgt tatcaaataa
    11521 caaataaata agtaagacaa ttttgaaaat gagttgtgtt cattctgcta caaggacttt
    11581 gcacttaatc gaaattattt tttattcttt tgaaaatcaa aatactatag ttgcaatgta
    11641 ccaaatttga agaagtataa ataaccttta acttctttat taagaatcgt ttgaagcgta
    11701 ttttgataat atttcatctg tatcttatat ttatttttta attgtgtacc aatttcttca
    11761 tctgtcatcc cacggcgacg attaaatgca tcggttttat agtctacaaa ataatgcaca
    11821 ccatctttaa caaagattaa gtcaatcata ccttgaataa ttgagacgtc ttcgtctcct
    11881 tgtggcaatt ggtcaactaa tgcttggtta actacaaacg gtaattcacg ataaacttgc
    11941 tctgcttcag caataatcga atataactca ctattgataa atgtcattat ttcatccata
    12001 cggatatctt ttttcgcatc tgcttcgata atatgtttat cgattaatcc atcgatatac
    12061 tgatgtaact caacttcaga tatgcgttct tttttgaatg gtaaatgttg catcactgta
    12121 tgcattaacg taccaatttc attcgctttt cgtttacctt gttcacttag aaatttaggt
    12181 cgttcatacg ttgaaaaacc gatacgatat tgccttactc gttcgtaact tgtgccactt
    12241 tcttctgttt catattgtct tttcaattca gaaacagatt gttttgaggg ctttttagta
    12301 tcatttacat atggatatcg ataatcaagt tggtgtttaa tttgtgcttt aacatcttca
    12361 ttaccatttt gcatagtttc taattgatta accgaacgat attcatcatt atctaaaatg
    12421 gtttctgtag acacatcttc aaagtacaca attgaaatat ttacattcgg acgactacta
    12481 tcttcaattt gtgctatatc tttttcaaat tttaaatcat ctggaattga cgcagattga
    12541 tgtttagata aaatactata aataagatgg aacggatttg gtgaagttaa tcgttcattg
    12601 acagcaatgt gctcaccaga aatagacaat tgctctagtt ctagtaatga tttatcattt
    12661 ttcactctac caattaaata aagttgttct ttcgctcttg ttaatgctac atagactaat
    12721 cgcatttctt ctgacacaag ttctttttcg gcaacagctc tatatgcaac cgaagctaaa
    12781 gatggaaatg ccatttcttt atccacatca aaataatcca ttccgagacc aaattgctga
    12841 tttaaaataa ctggttgttt caaatcacgt ttattaaaat cttttgacaa tccagaataa
    12901 atgacaaatg gaaactctag acctttacta ctatgaattg tcatcattct aacgacatta
    12961 tcgtttggac caactacatt ttcctcacca aaatctttgc ctctttcaat caattcatcg
    13021 ataaaacgaa taaattgata taaacctcta aaacttgaat tctcaaactc gatagcttta
    13081 ttaaataaac cataaagatt tgcacgtcgt ccacgtccac caataagtcc actaaagtat
    13141 tgaataacat aatgatcatt ataaaattta tcaattaact gatacaccgg atgatcttta
    13201 ctatattgtt ggtaactttg aatatctgat aaaaacattt ttaatttatc aactaaaatt
    13261 gcatctgctg cttcgtcatt aatgtaattt acaatcgatt gatagaagta gtcatcattt
    13321 ggactcaata ttctaatttg agctaattcg tcttctttga actgatatat aacggagcgc
    13381 attaacccaa ctaaataaat atcttgtaat ggattatcta tcgctcttaa aaatgataat
    13441 actaagcgga cttctgtttg ttcaaagtaa ccttcacgac tattcacatg gaatggaata
    13501 tcttcatttt taaaggcttg ttgtaaattg cgagcttgtc caaagctgcg ttctagaata
    13561 acgatatcct tgtatgtcgc acttctatag cttcctgttt tcatatcata aactttttga
    13621 tgttctaaga tatctttaac ttgttctact ataaaatgcg cttcttgttc actaccagtt
    13681 aaatcactat gttcttgatc cgcttcaaca aggactttta agtttactgg atggtccgat
    13741 tcatcatatg gtgcaccata atacaactgt gccgcttcat catattttac ttcaccgact
    13801 tgttcatcca tcatatgttt gaatatatag ttagtcgttg acagtacttc ttttcgagaa
    13861 cggaagtttt gcgacaaatc aattcgacgt ccagtgccat ctccatctat agtaaagcgt
    13921 tgatactttt caataaataa acttggatca gcttgtctaa atttataaat ggattgctta
    13981 acatctccaa ccataaataa attaccatta tgttcatcac ccgttttgat gcaagatagt
    14041 attttctctt gaactcggtt cgtatcttga tactcatcga ccaatatttc ttggaagtgt
    14101 tgacggtatg attcggcaat ttctgaaggc gaaccatcct cattagttaa aatttgtaat
    14161 gcaaaatgtt cataatcaga aaaatccaaa atatttttgc tacgcttttt tcgattgaat
    14221 tctgacataa catctttcac aatacgcgca aggtacttta ctcgtggcgc caattgttgc
    14281 atatcagctt tcaaatcatc agcttctctt gaaaagtaat cactcttcac tttatcaatt
    14341 aatgatttat attttttata ctgaagttta gcatcttcta aggcatcgac catcatttca
    14401 tttgcttctt taattttcgc tgttacatta ggcaaacgcg cgccaaattc gtgaccagtt
    14461 aaataaggta tatcaataaa gccaccttct aaaacacgcc ccattaaacg tcgttcatct
    14521 tctataacag ctaaatgttt atcgacgcta tccatcatac taaacaaatc atacgcctta
    14581 tttaaagcat cataagcagc tgtgataaat actttagata agtctgttag tagttgaata
    14641 agttgcgctt gttgtgcttc ttcttcgtat ggtgtcacca attgatccaa ccaattttta
    14701 ggatttggat ttgcaacgct aaagaaatac aattgtttaa taatcattcg aaactgatca
    14761 tcacttctat ctgaagacaa ttgctctgtt aattcaataa aagcaggatc aaggatatcg
    14821 taatgttgtt ctatgacctc atctatcgtt tgttctaata ataaaatatt ttcagcttca
    14881 ctgcttgttc taaagttcgg gtcaatattt aatacatcat aatgctgttg aattaatttc
    14941 aagcaaaaac tatggagtgt agatatttgt gcttgatgaa ttttgatgcg ttggtttttc
    15001 aagtgtgcat ttgcaggatc agcaatcgat gcctcttgaa tacgttggtc tacacgatgc
    15061 ttcatttcac gtgcgcttaa gtttgtaaac gttacgacta aaagtcgatc gacatcaatg
    15121 ccatcacgta aaatcttttg gataatacgc tcaactagta cagctgtttt acctgaacct
    15181 gccgcggctg caacaagtac atcttgtcca gttgcgtaaa tactttgcca ttgcgcgtca
    15241 gtccaaatca cgccttgtgg tttctctgga attgtcatct attgctcacc cccaaattca
    15301 tcattaatgt taatattttg aattgcttca attggattta ttgtttcatc tacagttcga
    15361 tatcgcttac tatcaatcat gccatctaca tgacataccg attgataact acaaaaagca
    15421 catggcaatt tttgtttgta ctttaatggt gcaacttcag tatgtccatc cataatattt
    15481 gaagctgttt ctataaaatt ctctttgtta tgttggatga atttataaat cgttgcttca
    15541 tctgccactt ggctgcctcg tttactcaaa gagccatctt tattcaaacc aactggtaca
    15601 atatctgaag tgaatttagg ttctaaacga atatccaatg catcaataac agtttggtct
    15661 gcattaacta aaccactcaa cttaaacttt ttaattaaat cttgttctag tttatcttca
    15721 tcaatatcag accatgattt aaatttaatt ctaggttcat gtacatggaa gtataataat
    15781 ccacctggtt tcacaatatc tgttaatcca aggcgttgtt tattttgtaa aacgatatcc
    15841 atgtatgtca tcatttgcat ttgcatacca taatatactt tcgttaaatc aagtgtcgca
    15901 ctaccttcag aggatttata gtcaatgata ttaacaaaac ttgtatcatt ctttgtatac
    15961 gtatcgatac ggtcaatttg ccctctaata ttaattggaa taccttgagt tgtcgttaat
    16021 gtttgtgcaa ttaattcgtc atttgttctt ggtttccttc taaaacttgt ctcaaaatgt
    16081 tttggcataa actttgaata cgtgccttga tattttaatg cgcttagtgt tgtttctaca
    16141 atagcgccaa tgcgtcttga taaataacga tagtaagctg aagaatttaa taaattaaac
    16201 tgaactttag gtaaaatttc ttccaatgct tcattcgtta attgtcttat ttttttcagg
    16261 tctaattgtt taaaatcgcc attaatacgt tcagatatat attttaaaac agaatggaaa
    16321 atatcaccta aatcaaagtt ttgaagttca tacttcgttc gctcatttag tttcagacca
    16381 tgtgacgcat agtgtttaaa tgggcattgt tgataacctt caaaacggga tacactggca
    16441 ttgatttcct taccatataa atctttagac aacgtttcac ctaattttac agtttcattg
    16501 tcaaacgtta atgctgacat taaataatct aaaccttgat ttaaatgatc gctatctcta
    16561 attacttgat aagcatctaa ccaactatca gccacaattt catcatataa ccaagcacgc
    16621 aatgcttcaa ataatgtaat tttggtttgc ttagcatgtt gcattagtga caatgggtta
    16681 acttcatggt attgaggaat gttagtaatt tccaattggt tgaacaatga ttgaatttga
    16741 tttaaaaatg ggctgatctc cttatcatca ccacttgatc ccattagact gtaagaaaat
    16801 gtaacatctc ccttagctct agtcatagca acataacaaa caaatgcttc atccatctgt
    16861 aaaatatctg atgttggact taactcgaca ttagcctgct gttcaaagta tttcttttct
    16921 tcatctgtaa tcaagcttga cgcagttact ggttgtggca tcgttccatc attcatacct
    16981 actaagtaaa catgttgctt attatcgact ttagccaaat ccatcgtacc aatacttact
    17041 tggtccaatg tttgcggaat cataacaaat tctaattgtt ctaaaccaat atcaaatact
    17101 tctaagaaac gttccatcga cattggttca tctccaaata ctagaactaa gtcatcaagg
    17161 atttgaatta agccattcca tatttgatca atttcctccg ccttttcatg attaccattt
    17221 aaatcaagtt catctcgctc tgtcatcaat tgatttggca gttcgaaata ttccatactt
    17281 tcataaaaag cagttgcaaa atcttttaca gtttccgctt gtgacatttg cttttcaaaa
    17341 tgtaaaattt tatcaatgac atctttcttt aacttaacga cttgttcaaa tgtgttacgt
    17401 tcatcttcgg tcagtttatg cgctttacgc cccattttgc taaaatgttc gacattaaat
    17461 agctcatcat ctaaccaacg tttaccgtat ataccacgtt caagtacaaa attttcaagt
    17521 aaatcaacta agtatgcact ttttagatat gatgccgtta acacatcagt cttcaataag
    17581 cgtagcattg gattcacttg ccaattagat tgaataactt caatcaatga acgaatcatt
    17641 tccatgaccg gatgatgtgt catcgaacgc tttgtatcaa tattataagg aatattataa
    17701 agcggtaata tggaatcaaa taaataagca taagattcat cacgatataa aatagcaata
    17761 tcttgatatc gtaattgctt atcacgaata tcaacgatga tacgtcgcgc aatttcattt
    17821 atttcctctc tcatagtcgc agattctaaa atattgatat gaccttgaca tgccactcga
    17881 ttgatttgaa gtgcatcaaa ttcttgttca agatgcttta aatcttgatt attgaagcga
    17941 tataattggt tgaaatattg acgttcaata gaaatattga gttcatttgc tatttcttca
    18001 atatgtcgta acacttccga tggttttcta aataaactaa attgatcgtg gttaccatct
    18061 gtcgtcaata taattgtgac actcttcgca tatttaatta atcctttgat tattaaatac
    18121 tcaatcgttg aaaagttgtg aaaaccatca atatatatat cagcacgttt tagccactct
    18181 gatttcggca tacaatcaat aaaatattgt aatgaatcct cacccgtaat aaactcgttt
    18241 tgaatgcgtt gttcgaactc acggtatatt aaagcaatat cctctaactt atttttagtt
    18301 cgagtttgca tatttttatc agcaataaag tgttctaaat gttcaggcgt tactgcatat
    18361 tttttaaaat cttgaatttg ttctgttaat ttttcactaa acccataata ttttgcttgt
    18421 gattgataaa gttttaaata cttttgttgt tcttgaacaa tgttataaat catcatttcc
    18481 gttgcagctt tagataactt ttgttcgcta taactaccaa cttcttggaa aatacgatga
    18541 cttaatcgtt caaaatgcaa cacttctgtt cttaaactac catttaattc cggatcattg
    18601 acaaaggctt gttctaattg aaatgtactt tgagttggcg caattaaaat gattggatct
    18661 ccaagcggat ctgctttcat tttttgtttt atttcggtca acattttcgt agacttacct
    18721 gttcccgctc tacctaaata agcatgtaat gtcatgacaa accctcactt cctttgacac
    18781 atattttaac acaaagtgat tgtgattgat ttattataag ttcatttcga acttagcttt
    18841 tattgtggta ctaacaaaat aaaacatcgt ttatcagtat taaaaccgac aaacgatgtt
    18901 gtatttgttt catattaatt tttagtattt tcaggattga aattatgttt aaattcacta
    18961 aatggccaga acctaaatga aactttacca acaatttggt cttcatcaat gaggccaaac
    19021 gcacggctat ctttacttac ttcacgatta tctccaagca ctaaatattt accttttgga
    19081 atgacatttg atttaggatt cgcattcggt aaatctttaa cttggaaagt cccagtaatg
    19141 taatcacctt gtttatgttt taaattgtag tttaaatatg gttcatcttg ttttttacca
    19201 ttgacatata atgtatcatt tttgtattct actttatcac caggaacacc gatgacacgt
    19261 ttaacatagt catcattttt gtttgcatgg aagacaacta cattaccttt ttccaaacca
    19321 cctgttttat atccaacaat gtttacagct actcgctcgc catctttcaa agttggatcc
    19381 attgattcac ctttaattgt atatggcgta acaataaatt tacctactat aaataaaatg
    19441 acaaaagcga ctgcaattga aataatccat tccaatattt cttttttcaa ttttgacacc
    19501 tctttttaag atttgaactg aacagtccat tttgaaaaag gatagtatcg taaactaaca
    19561 ttaccaataa tatccttttt atcgattaaa ccaaattgtc ttgaatcgtg cttgttatta
    19621 tcttgatcat ttagcacaac aaaattgttt ggcggaataa tatcaccatc taattcttta
    19681 aaattgcgca aactaaaatc tttaattttt ctgttcttgg cataagatgc gtcaaccggt
    19741 cggtcatcac ggtataattg tccctgacga aacgccattg attgaccagg tttggcaata
    19801 attcgactag tatatatctc gttaccacgc ctatatgtaa tgatatcacc attattcaat
    19861 tgattaaatg taactttaat tttatttaca ataacacgat cccctttgtt aagggttggt
    19921 gacatatcat tattcggaat gacatgacca actattacaa aagtttgtac gaacagtaca
    19981 atgataatag caagtatcaa tgaaatcaaa tattttacaa cttttttcac gatgtcactc
    20041 ctttttcgat cccataaaat gctgttctaa cttttttcca acaatccaaa atacaaccaa
    20101 cacaactaac attaatatcc ctcttaaagg atgcgttaaa attgtagtaa tttccttacc
    20161 taaataacct aaaataattg ttgaaactaa ctttgatgat gccaaaacaa tgaaataata
    20221 tttaggtcta atatgagata gactcgctac aaaatttatt aatgtatttg gcgtaaaagg
    20281 aaaacaaagt aaaataaaca atgggattaa tccttggcga tcaataaaac taatcaagcg
    20341 ttgaacagca gtacgttgtt taattcgctg catcctctca gtgttcacca atcgtttaca
    20401 gatcaaatag actgtaaatg ttccagaaat taatccaagc caactaatca atatacctaa
    20461 aataggtcca taagcttgaa tgttaattaa aatatagagt gctaaaggaa atactggaat
    20521 tatagctcta atatataaca atataaatcc aggtaaataa ccaaactgtc gaaatatctc
    20581 aaaccattct tctacttgat gaaacgacaa atcatcaatc cctttctttg gttgaagata
    20641 attattctta cattataaag ttaattacta agtaaagaaa cattaacatg taatcatgct
    20701 aataaatcag tctctagtag tataacaata ttattgtaag gcagatatta ttttatcttt
    20761 aattttctct taacatcttg tcacattagc tagtaaccta gttcttgcat ataaataaaa
    20821 acgaggttgt cattacattt ttacatttgt aatgacaacc tcgaaaatat gctttaaata
    20881 gttaacgtct agttctatct tttcaaactt cactaatctt tgaagtaatg tattttataa
    20941 acgttcttct aattcttttt tcaagtcttc aaaaccaggt ttacctaata atgcgaacat
    21001 gttttgttta tatgcttcta caccaggttg gttaaatgga tttacgccta attggtatcc
    21061 actcattgca caagcaagtt cgaagaagta tacgacgtaa ccgaaagttt cttcatctaa
    21121 ttgtggaatg ttcactacca tgttaggaac accaccatca gtatgcgcta ataatgtacc
    21181 ttcgaatgct tttgtgttaa cttcgtcgat tgttttacca gctaaataat ttaatccgtc
    21241 tagatcatca ctatcttttt caatagtaat atcatattta ggatgattta cttttacaac
    21301 tgtttcgaat aagaagcgac ggccttcttg tacatattga cctaaagaat gtaaatcagt
    21361 tgtgtagttg gcacttgaag gatagatacc tttgaagtct ttaccttctg attcaccaaa
    21421 taattgtttc caccattcat taaagtattg catagatggt tcatagttaa tcaacatttc
    21481 tgttgtataa ccttttgcat ataaaatgtt tcgaattgtc gcatattggt atgcaatgtt
    21541 ttcttctaat ttatctgaag ataattcttc acgtgctttt gcagcaccaa tcatcatagc
    21601 ttcgatgtta attccagctg ttgcaattgg taataatcct actgctgtta aaacagaata
    21661 tcttccacct acatcatcag gtacgataaa cgtttcataa ccttcgtttg tagccaactg
    21721 ttttaaagca cctttttctt tatccgttgt tgcaaatata cgtttttgtg cttcttcttt
    21781 accgtatctt tcttcaacta attgtttgaa caatctaaat gcaactgctg gttctgtagt
    21841 tgtaccagat ttagaaataa cgtttacaga gaaatctttg tctgctaaat aatcaactaa
    21901 ctctttcgta tatgttgatg ataagtgatt accaacaaat acaatttcag ggtattcatt
    21961 gctgtttcta aatgatgacg ttaacatttc gattgctgca cgtgcaccta agtaagaacc
    22021 accaataccg atgactacta aaacatcaga attttcttta atgcgttttg atgcttcaac
    22081 aattcttgaa aattcttctt tgtcgtaatc aactggtaaa tcaacccagc ctaagaagtc
    22141 actacctgca ccagtacctt catgaattgt tttgtgaatt gatttaacaa tttcttgttg
    22201 ttgttttaat tcgtgttcac cgaaaaattc taacgtttta ctaaaatcta attgaatatg
    22261 agtcatctat aaaagcctcc tgtttcattt aatatcttaa ttttactaag tttacgtttg
    22321 cgattcaatt cacaagtttg tacttaatca ataaaattga tatttatgtg acgtttcaca
    22381 cctatttttc aacaaacatt tcaaactggc gtcaaacact caaacatttt ttatacaatt
    22441 tgcatgtttt ttaaaatact tttaaacatt gataaataag catttacact tacaactcta
    22501 actatctcta tcgcatttta tgaataaata tgtaaaataa tggttttatt ttcattaaaa
    22561 gtttgctatc atgtgttcat acgaaatatt cagataacta caatgaggtg aattttatga
    22621 aagagaaaat tgttttagca tattcaggag gactagatac aagtgtggcc gttcaatggc
    22681 ttatcgacaa aggatacgac gttgtagctt gttgcctaga tgttggtgaa ggtaaagatt
    22741 tagacatcgt ttataaaaaa gctttagata tgggagcagt tgaatgtcat attattgatg
    22801 caacaaaaga atttagtgat gagtatgtaa gttatgcaat caaaggaaat ttaatgtatg
    22861 aaaatgcata tccattagtt tcagctttat caagaccatt aatcgctaaa aaattagtag
    22921 agattgctga gaaaacaaat tcagtaggta ttgcacatgg ttgtactggt aaagggaatg
    22981 accaagtacg tttcgaagtt gccattaaag cattaaaccc atcattgaaa gcattcgcac
    23041 ctgtacgtga gtgggcatgg agtcgtgaag aagaaatcga ttatgcaatt aaacataata
    23101 tccctgtatc aatcaaccat gattcacctt attctatcga tcaaaatcta tggggcagag
    23161 cgaatgaatg tggtatttta gaagatcctt atgctgcgcc accagaggat gcgttcgatc
    23221 taacaaatgc tttagaagaa acaccagata ctgctgatga aatcatttta acgtttgata
    23281 aaggcatccc agttcaaatt gatggcaaaa catatgaatt agacgattta attttaacgt
    23341 tgaatgcatt agctggtaag catggtatcg gaagaattga ccatgtagaa aatagacttg
    23401 taggtatcaa atcaagagaa atttatgagg cacctgctgc agaagttatt ttaaaagcgc
    23461 ataaagcatt agaaacgatt acgttaacga aagatgtcgc acactttaaa ccaatcattg
    23521 agaagcaatt tgctgaacaa ctatacaatg gactttggtt ctcaccttta actgatagct
    23581 tgaaattatt tattgatagt actcagcaat acgtaagtgg tgatgtcaga attaaattat
    23641 tcaaaggtaa tgccatcgtg aatggtagaa aatcacctta cacattatat gatgaaaaat
    23701 tagcaactta tacaaaagaa gatgcattta atcaagacgc tgctgttggc tttatcgata
    23761 tctatggttt acctactcaa gtaaatgcga tgttgcatgg aggctatagc aatgagcaat
    23821 aaagcttggg gcggtagatt tgaagtacaa cctgaagagt gggttgacga ctttaacgca
    23881 tccattactt ttgatcaaac gctcatagat caagatatcg aaggcagcat tgcacatgca
    23941 actatgcttg cgaatcaagg cattattagt caacaagaca gcgaacaaat tatacaagga
    24001 ctaaaatcta ttcaacatga ttatcatcaa gatcaaattc aatttagtgc atcattagaa
    24061 gatattcatt taaatattga acatgaatta attaaacgta tcggtgatgc tggtggtaag
    24121 ttgcatactg gacgcagtag aaacgatcaa gttgcaacag acatgcactt gtacactaag
    24181 aaacaagtgc aagatatcat cgcattaatt aagtcgttac aaagtgtaat tgtagacatc
    24241 gcttccaata atgttgatac aattatgcct agttatactc atttacagcg tgcacagcca
    24301 atttcatttg cacatcatat tatgacttat ttttggatgt tacaacgaga ccaacaacga
    24361 tttgaagata gtttaaaacg aatcgatatt aatcctttag gtgcagcagc cttaagtggt
    24421 accacatacc ctatcgatag acacgagaca acagcattgt tgaactttgg cagtctctat
    24481 gagaatagcc tagatgctgt tagtgacaga gactatatta ttgaaacatt gcataatatt
    24541 tctttaacga tggttcactt atcacgcttt gcagaggaaa ttattttctg gtccacagac
    24601 gaagctaaat tcattacatt atcagatgca ttttcaactg gctcatctat tatgccacaa
    24661 aagaaaaatc ctgatatggc agaattaatt agaggtaaag ttggtcgaac gactggtcat
    24721 ttaatgagca tgcttatgac tttaaaagga ttacctctag catataacaa agatatgcag
    24781 gaagataaag aaggtttatt cgatgctgtc catacaatta aaggttcttt acgtattttc
    24841 gaaggtatga ttcaaacgat gacaattaat aaagaacgac tcaatcaaac tgttaaagaa
    24901 gatttttcaa atgcaacgga actagcagat tatttagtaa ctaaaaatat tccatttaga
    24961 actgcacatg aaattgtagg aaaaatcgtc ttagaatgta tacaacaagg tcattattta
    25021 ttagatgttc ctttagcaac atatcaacaa catcattcta gtattgatgc cgatatttac
    25081 gattatttgc agcctgaaaa ttgtttaaaa cgacgtcaaa gttacggttc aacaggtcaa
    25141 tcatcggtca aacaacaact tgatgttgct aaacaattac tatcacaata aatacgttaa
    25201 tctacctacc cacaatgtct attaaaatta cattgtgggt attttaatgc tctcttcgtc
    25261 ttgttgaaca tcacattttt aagattccta aaatgtttga taattctttt aaatttatat
    25321 tacaaaaatg ttataaattg taaaagaaat gtgtaaagcg ttttcacaag caggtttttg
    25381 tagtatttta aaattgttag actacaaata aagagatgaa aggataaaga ctatgactaa
    25441 ctcttcgaaa agcttcacta aatttatggc tgcttctgct gtttttacta tgggattttt
    25501 atcagtacct actgctggcg ctgaacaaac aaatcaaatt gcaaataaac ctcaggctat
    25561 tcaatggcat acaaatttaa cgaatgagcg attcactact atcgcacatc gtggcgcaag
    25621 tggctatgca cccgagcata cgtttcaagc atatgataag agtcataatg agttaaaagc
    25681 atcttatatc gaaattgatt tacaacgtac caaagatggc catttagttg ctatgcatga
    25741 tgaaactgtt aaccgtacaa caaatggaca cggtaaagtt gaggattata cccttgatga
    25801 attaaaacag ttagatgcag gaagttggtt taataaaaaa tatccaaaat acgcaagagc
    25861 aagttataaa aatgctaaag tacccacttt agatgaaatt ttagaacgtt atggcccgaa
    25921 tgcaaactat tatattgaaa caaagtcacc tgatgtatac ccaggaatgg aagaacaatt
    25981 attagcttca ttgaaaaagc atcacctttt aaataacaat aaattaaaaa atggacatgt
    26041 aatgattcaa tcattttctg acgaaagttt aaagaaaatt catcgtcaaa ataagcatgt
    26101 gccattagta aaattagttg ataaaggtga actacaacaa tttaacgacc aacgcttaaa
    26161 agagatacgc tcttatgcga ttggattagg tcctgattat acagatttaa ctgaacaaaa
    26221 tacccatcat ttaaaagact taggatttat agtacatcct tatacagtga atgaaaaagc
    26281 tgatatgtta cgattaaata aatatggcgt tgatggtgtc tttacaaatt tcgctgataa
    26341 atataaagaa gtcattaagt agtaatgtta aactagaaaa cataaataca aaaatatagc
    26401 tattactata aaaaacagca gtaagatatt tccaaattga aattatccta ctgctgtctt
    26461 tttgggagtg ggacagaaat gatattttcg caaaatttat ttcgtcgtcc caccccaact
    26521 tgcacactat tgtaagctga cttttcgcca gcttctgtgt tggggccccg ccaacttgca
    26581 ttgtctgtag aaattgagga gctaatttct ctgtgtcggg gctccacccc aacttgcaca
    26641 ctattgtaag ctgactttcc gccagcctct gtgttggggc cccgccaact tgcacactat
    26701 tgtaagctga ctttccacca gcctctgtgt tggggccccg actatttttg aaaagagcgt
    26761 gttacacggg cattgtttta cagtcaacta ctgctaaaat aaaattaacg agcttagggc
    26821 tttgttttct gtcccaagct cgttaaatca catatgataa ttaattatgc ccaaccacga
    26881 tatctagctg cttctgctgt acgtttaata cctatgatat atgctgcaag tctcatatct
    26941 atttttcggt tttgagacaa ttcgtaaatc gtatcaaatg ccgcttctaa tttttcacgt
    27001 agcttttcat caacttcttc ttcagaccaa taataacctt gattattttg tacccattcg
    27061 aagtaagaaa ccgttacacc accagcactt gctaatacgt ctggaactaa taatatacca
    27121 cgttcagtta aaatacgtgt tgcttctggt gttgtaggtc cattagcagc ttcaacaacg
    27181 atactagctt taatatcatg tgcattgtct tctgtaattt ggtttgaaat agccgctggt
    27241 actaaaatgt cacaatctaa ttcaaacaat tctttatttg agattgtttc ttcaaataaa
    27301 tttgttaccg taccaaaact atcacgacgg tctaataaat aatctatatc taagccattt
    27361 ggatcgtgta atgcaccgta agcatcagag atacctacaa tttttgcacc taaatcatat
    27421 aagaatttag ctaagaaact tccggcatta ccgaaacctt gaataacaac cttggcacct
    27481 tcaatttgca tattacgacg ttttgcagct tgttcaattg caataactac acctagtgca
    27541 gttgatctgt cgcgtccatg agaaccaccc aatacaattg gtttacctgt gatgaaacct
    27601 ggtgaattaa atttatctaa tgcactatat tcatccatca tccaagccat aatttgtgag
    27661 tttgtaaata catctggtgc tggaatatct ttgttcggac ctacgaattg tgaaattgct
    27721 cttacatatc cgcgtgataa acgttcaact tcatgaatgc tcatttgacg tggatcacaa
    27781 acgataccac ccttaccacc accgtatggt aagtttacaa tgccacattt caaagtcatc
    27841 cacattgata atgcttttac ttcttcttca tcaacatctg ggtggaaacg cacgccccct
    27901 tttgttggtc caacagcatc attatgttgc gcacggtaac ctgtgaatgt ttttactgtg
    27961 ccatcatcca ttcgtacagg gatacgcact tgtaacattc ttaaaggttc tttaattaaa
    28021 tcgtacattc cttcgtcaaa tcccaattta tgcaatgctt ctttaataat tccttgagta
    28081 gaagttacta aattattgtt ctcagtcatg atccttttcg cctcttcttt acctaatgat
    28141 ttcgctttca aacatattgt aacataacgt attccttttt aaagccctta caaactgatt
    28201 gttacaactt tttgacatta ttgaaataca tgtcttattt tttcaagtgc aaggtccaat
    28261 tcttctttag taataattaa tggtggtgca aaacgaatga cagtatcatg cgtttcttta
    28321 cataataagc cttcttcttt caaagcttca caatatggtc tagcactttc attaagttcc
    28381 acacctataa acaaaccacg tccacggact tctttaattg atggatgatc aatttgcttt
    28441 aattgttctt taaaataatc tcctaattct aaagagcggc ctggtaaatc ctcatcaacg
    28501 ataacatcta atgcagcaat tgatgcagca caagcaagtg gattaccacc aaatgttgaa
    28561 ccatgtgagc caggtgtaaa gacatctaat acttctttat ctgctaatac aacagaaatt
    28621 gggaagactc caccacctag tgctttacct aaaatataga catcaggttt tacattatcc
    28681 caatccgtag caaataattt acccgaacga cctaatcctg cttggatttc gtcagcaata
    28741 aataagacat tatgttcatc acataattct ctaattgctt tcaaatatcc ttctggcggt
    28801 atatttatac ccgcttcacc ttgaattggt tctactaaaa ctgctgcagt attttcatta
    28861 attgcagctt tcaatgcatc tacatctcca aaatcaactt ttctaaatcc atctaataac
    28921 ggaccataac cacgttggta ttctgcttct gaagataatg aaactggcgc cattgttcga
    28981 ccatggaagt taccattaaa tgcaatgatt tctgctttat ttggctcaat tcctttaaca
    29041 tcgtatgccc agcgtcgtgc tgctttcaaa gctgtttcta ctgcttcagc acctgtattc
    29101 attggtaaag ctttatcttt acctgccagt ttacaaattt tttcgtacca ttcacctaag
    29161 ttatcactat gaaaagcacg tgaaactaaa gtcactttat cagcttgatc ttttaatgct
    29221 tgaataattt tcggatgtct atgaccttgg ttaacagcgg aatatgcaga taacatatcc
    29281 atatatttat tgccttcagg atctttaacc catacccctt cagcttctga aatgacaatt
    29341 ggcaatggta aataattatg tgctccgtaa tgatttgtta actcaataat tttttcagat
    29401 ttagtcatca tatctcccct tttcatcatt tataactatt atacatgaaa cattatccaa
    29461 ataattacat tagttttcaa agcagatact tttccaccaa aaaagatgaa ataatcacta
    29521 agtttcatta aatttgtcta ttttgaaaac ccttacattt ataatgacat aattacttaa
    29581 atgattacaa gcaaaagaat tgataatttt acacttaatc aaaagtatat tttactaaga
    29641 atatttttat ttataaatat tgaaaaccac taacaaattg catacacaat atcattagtg
    29701 gtaacagtta aacacttatt tatctttacg tggtaatgga taaaaccctt cattaaattg
    29761 atgccataaa ttaggagtta agtgatgatt atcttctctc tctggatcaa agtaattaat
    29821 aatttcatct tcccgtccat ctttaatttt ttcaacaaat tgataatcca gtagtagctc
    29881 tctaccaatg gctactaagt caacaccaac attttcaact gcatctaaag cttcgtcagc
    29941 tgtgaaaatt gaaccaatac cgataagtgg catacgacca tttatccatt tgtgaattaa
    30001 aggcagtctt tcttgtccag cgtatttacc ttcacgtgtc gttgcatgcg tatccatcat
    30061 tgaaacatga atatagtcga ttggcatatg gctaatttta ttaacgagtt cctctgtaat
    30121 ttccattgtg attcctggag actccgcttc ctctggagat aatctgtatc caactataaa
    30181 gtctttattg ccatacgctt ctttcgcttt aagtacttct tcaatcacag cgaccgggaa
    30241 tttatattga tttgcccata catcatttct tctattatag tatggtgata caaattgatg
    30301 aattaagtaa tgattcgcgc catgtatttc aacaccatca aaccctgctt caattgcacg
    30361 tcgcgttgct tcaccaaaat ccttgattgc ttgttcaatc tcttcattcg tcatttctct
    30421 agcactatgt tcttgtttct gaccaaaact ttttaaagaa attggacttg gtgctacgac
    30481 gtctccatca ggtgttaatt caggcaatgc ttgtgcaccg ccatgatgta tttgtacgag
    30541 tgctttggca ccgtttttct tcattgctgt agctaatcgt tttagtcctt caatattact
    30601 gtcatgcgcg attgatggct gtcctggaaa tgcttttccg acatcactca cattactcgc
    30661 agcattaatt gtaataccaa catcttgtga acgcttttca atataaggaa gttctacatc
    30721 tgaaatagta ccatcatcat ttgaagaaat atgtgttaaa ggggctaaca caaatcgatt
    30781 tctcaactct actccatttg gtaattctac tttatcaaac aatggttcgt atttactttt
    30841 cattacttaa ttcctcctta aaattattga gttgactata acatgtaatt ttttcattct
    30901 ccaattatct gtttatatag ttgcaattcg tttaatactt taagttgtag ttctcatttc
    30961 taaaataaat aaaaaaaaca aatccacata caatattgca aatcaacgat tctatctaac
    31021 aaagataatg gatatgtcac cttaaaccat ttgtacgtgt aaatagaatc aatcatatta
    31081 cgttatcgta gacaaaaaga gattagtatc taataaaatt taatagatac taatctcttt
    31141 tatagtctaa gaaactttca tttcagaccc tttgtatgat tttcggtacg atttatctgt
    31201 tcctttagtc gtttcgaatt gctcgctttg actgttttat ccagattggt aagcgttctt
    31261 taagtgtttg aaacccatat ttttctgttt ctctgatttc atctaatact gattgttttt
    31321 ccttcttacg ctcataattt ttgaagtaat cattatcctt taatgataga ttaagctttc
    31381 cttcatcatc tatagacaaa attttagctt taacaatttg gccttctgat agaaatttct
    31441 tcaaattatg aacgtagtca tccataattt ctgatatatg aatcagtcct tcagtatgat
    31501 taggggtctc aacaaacgca ccgtatggtt gaataccagt tacacgcacc ttgatatgtt
    31561 ggccaatttt gtagttattc aacttaataa ccccttattt cgtttactta acactattat
    31621 catagcacaa atcgaaatgg tggactaatt aaaacatcaa atcatacgaa tattatttta
    31681 taaatgaata agaattctac aaacttggat ttacaaataa aattagcaac aaattcctat
    31741 ctaaatcaat atgatatcca gctactttgt tttcaattta tttactattt gaatagcaat
    31801 tctctgtaaa aacataaaaa gcaacctcaa ctattatctt taaaatacac atctatttaa
    31861 aaactgtgct ttcttgataa atatattgag attgctcatc atattagttt att
//
LOCUS       DANJFZ010000022        30684 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.022, whole genome shotgun sequence.
ACCESSION   DANJFZ010000022 DANJFZ010000000
VERSION     DANJFZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.022"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(86..1051)
                     /locus_tag="PWN45_000851"
     CDS             complement(86..1051)
                     /locus_tag="PWN45_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL0622634.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(1265..2902)
                     /locus_tag="PWN45_000852"
     CDS             complement(1265..2902)
                     /locus_tag="PWN45_000852"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDL0622635.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(3062..3940)
                     /gene="hchA"
                     /locus_tag="PWN45_000853"
     CDS             complement(3062..3940)
                     /gene="hchA"
                     /locus_tag="PWN45_000853"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDL0622636.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(4206..5393)
                     /locus_tag="PWN45_000854"
     CDS             complement(4206..5393)
                     /locus_tag="PWN45_000854"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL0622637.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            5564..6739
                     /locus_tag="PWN45_000855"
     CDS             5564..6739
                     /locus_tag="PWN45_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0622638.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(7021..8205)
                     /gene="tuf"
                     /locus_tag="PWN45_000856"
     CDS             complement(7021..8205)
                     /gene="tuf"
                     /locus_tag="PWN45_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0622639.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(8422..10503)
                     /gene="fusA"
                     /locus_tag="PWN45_000857"
     CDS             complement(8422..10503)
                     /gene="fusA"
                     /locus_tag="PWN45_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0622640.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(10626..11096)
                     /gene="rpsG"
                     /locus_tag="PWN45_000858"
     CDS             complement(10626..11096)
                     /gene="rpsG"
                     /locus_tag="PWN45_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL0622641.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(11162..11575)
                     /gene="rpsL"
                     /locus_tag="PWN45_000859"
     CDS             complement(11162..11575)
                     /gene="rpsL"
                     /locus_tag="PWN45_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL0622642.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(11673..11927)
                     /locus_tag="PWN45_000860"
     CDS             complement(11673..11927)
                     /locus_tag="PWN45_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDL0622643.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(12064..15660)
                     /gene="rpoC"
                     /locus_tag="PWN45_000861"
     CDS             complement(12064..15660)
                     /gene="rpoC"
                     /locus_tag="PWN45_000861"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL0622644.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(15824..19375)
                     /gene="rpoB"
                     /locus_tag="PWN45_000862"
     CDS             complement(15824..19375)
                     /gene="rpoB"
                     /locus_tag="PWN45_000862"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0622645.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(19590..20198)
                     /locus_tag="PWN45_000863"
     CDS             complement(19590..20198)
                     /locus_tag="PWN45_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0622646.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(20373..20741)
                     /gene="rplL"
                     /locus_tag="PWN45_000864"
     CDS             complement(20373..20741)
                     /gene="rplL"
                     /locus_tag="PWN45_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL0622647.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(20784..21284)
                     /locus_tag="PWN45_000865"
     CDS             complement(20784..21284)
                     /locus_tag="PWN45_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL0622648.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(21322..21468)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21556..22248)
                     /gene="rplA"
                     /locus_tag="PWN45_000866"
     CDS             complement(21556..22248)
                     /gene="rplA"
                     /locus_tag="PWN45_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL0622649.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(22456..22878)
                     /gene="rplK"
                     /locus_tag="PWN45_000867"
     CDS             complement(22456..22878)
                     /gene="rplK"
                     /locus_tag="PWN45_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL0622650.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(23059..23607)
                     /gene="nusG"
                     /locus_tag="PWN45_000868"
     CDS             complement(23059..23607)
                     /gene="nusG"
                     /locus_tag="PWN45_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL0622651.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(23620..23802)
                     /gene="secE"
                     /locus_tag="PWN45_000869"
     CDS             complement(23620..23802)
                     /gene="secE"
                     /locus_tag="PWN45_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL0622652.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(23858..24001)
                     /gene="rpmG"
                     /locus_tag="PWN45_000870"
     CDS             complement(23858..24001)
                     /gene="rpmG"
                     /locus_tag="PWN45_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0622653.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(24116..24685)
                     /locus_tag="PWN45_000871"
     CDS             complement(24116..24685)
                     /locus_tag="PWN45_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL0622654.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(24766..25290)
                     /locus_tag="PWN45_000872"
     CDS             complement(24766..25290)
                     /locus_tag="PWN45_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL0622655.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(25290..26036)
                     /gene="rlmB"
                     /locus_tag="PWN45_000873"
     CDS             complement(25290..26036)
                     /gene="rlmB"
                     /locus_tag="PWN45_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL0622656.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(26044..26448)
                     /locus_tag="PWN45_000874"
     CDS             complement(26044..26448)
                     /locus_tag="PWN45_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL0622657.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(26441..27841)
                     /locus_tag="PWN45_000875"
     CDS             complement(26441..27841)
                     /locus_tag="PWN45_000875"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL0622658.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(27825..28472)
                     /gene="cysE"
                     /locus_tag="PWN45_000876"
     CDS             complement(27825..28472)
                     /gene="cysE"
                     /locus_tag="PWN45_000876"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL0622659.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(28895..30349)
                     /locus_tag="PWN45_000877"
     CDS             complement(28895..30349)
                     /locus_tag="PWN45_000877"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL0622660.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
BASE COUNT         9134 a         6034 c         4594 g        10922 t
ORIGIN      
        1 aataatatta gaattcatgc actagtttat taaaataaag agtaatttaa aatatcattc
       61 cgtgtattaa agtgaatgga aatgattagt tattattttt aacagtatct ttttgttcaa
      121 tagcttctaa cattaattta gtcatgctcg ctaaatcata tttaggatca aatccccatt
      181 cgccacgtga acagcttgta tcaatagaat ccggccaact attagcgata ccttgtctaa
      241 taggatcaac atcgtaatct aatgtaaaat tgggatagta ttcttgaatt gcttctttta
      301 ccatctctgg atcaaaactc attgcgctca aattataacc atttctagtt tctaatttag
      361 cgtcgtctgc ttccataagt ttaataattg cttcaattgc atcatccata tacatcatat
      421 ccatatacgt gcctttatct atgaagcttg tataatgacc ctctcttact gctttgaagt
      481 atatttcaac agcatagtct gtagtaccgc cacctggctc tttaacatgc gagattaaac
      541 ctgggaatct aacacttctt gtatctacac caaaacgttt gaaatagtat tgacacaata
      601 attctccagc tactttattt acaccataca ttgtcgtagg ttgctgaatc gttacttgtg
      661 gcgtattaac tttaggagtt gagtctccaa atgcaccaat tgaacttggt gtgaaaaagt
      721 gcaaattata agttcttgca gcttctaatg cattcattaa tccacccata tttaaatccc
      781 aagctagaat tggatttttc tcagcagttg ctgataataa tgctgccata tgcattagac
      841 tatccgcttc aaagtcccta actaactcaa acatacggtc acgatctgtt acgtctaaga
      901 tttcaaatgg tccattttgt acaggtgagt ctgcttcagg ttccctaata tctgtagcaa
      961 gaacattatc tgtcccataa atttctctgc acttaacaac taattctgta ccaatttgtc
     1021 ctaatgcacc agtaatcata atttttttca tagaaatatc tcctttgtct cttctatata
     1081 gctatagtcc atcacaagcg gacataatat tcattttcat aataattata atataaaagc
     1141 gctttcttgt atatatgaca tgtacatgtt gctgatattt ctgtaaatgg aaattctagt
     1201 tgtattaatt gattttagta atttatagcg tttattattg ctaattactg tcaaattaaa
     1261 ttttttatcc ctcaactctt aaactctgga tatctttcat tatattagct tttttataac
     1321 catggatatc atgtaaagcc ttataagctt taaataatgt ttcatacctt tgtacttttt
     1381 ccgcttctgg attaatttcg tataaaattg gctctttcat agcatcaact gcgtcattaa
     1441 ttgaattatg tgcgccacca ctgactgcac ctaatattgc tgcgcctatt gctggtgcat
     1501 actcactatc cataacaatt agttttttat tcagtacatt cgcatagata tccatcataa
     1561 cagcattctt tttaggtata ccgccacatg caaatacctt ttctacttcc atatgccaat
     1621 cttgatactg ttgcataatc atcttagtac caaatgctgt agcttctaaa tatgctctat
     1681 aaatatcctc atgcttagtt tgtaaagtta atccaaagat acaacctgtt aaattgctat
     1741 cacttaatac acttcgattt ccattatgcc aatcaagagc aatgagccca ctttcacctg
     1801 gcatttgatg ttttatcttt tcattcatta attcaaatac agtcatattt ctattttctg
     1861 cttcatctac atatgacttt ggtgcttgct tagcgacata ctcaaacaaa tcacctactg
     1921 ctgattgccc cgcttcataa gcaaataatt ctggaataat tgctcctttt acagaacctg
     1981 atatacctgg cacttgatgt tgcttttcat ttaacataag atggcatgtg cttgttccca
     2041 tcaccatagt catttcttta tctttttcag acccaatacc taataaacta gcatgggcat
     2101 caataataaa aggacttacc atagtttctt ttgataatcc taatttctgt gccattttat
     2161 catccagttt ccctactgct tcaccaatat taacaaccgg tgcagatact ttatcttgaa
     2221 ttacttttga taatttgggg tctattttat caaataaatc ataatgaaac cctgtttctt
     2281 cttcccaaaa tgctttgaaa cctaatccac aattcgagcg tacattttta ttagttaatt
     2341 tatttacaat ccaatcgccc gcttccataa tataagccgt tttttccata atttctggtg
     2401 ctcgattcat gacctccatt attttgggaa tcatccattc actactaaca ttatatccat
     2461 aatggcctaa ccacttatta ttattttcaa tagcagtttg atataatttc tctgcctctt
     2521 tatatgcacc atgatgtttc caaagtttca catacgcatg tggattgttt ttaaattgtt
     2581 ttaagttatg taccgggtta aggttttcgt cagtaaaaat aatagtagat gaagtaaagt
     2641 ctataccaat acctactata ttgtctggat ctatttttga ttcacgtact atatatgata
     2701 ttccttcttc cataatttct aaataatcat tactattttg aagtgcatat gtatgtggta
     2761 ttttcaaacc atttaattca ctctcaatga caccatgtgt atatggtttc acaaattttg
     2821 atactacttg accgttagtt gtattaatta aaaacacacg gcctgaagca gttccataat
     2881 caattccaat gctataagac atcatgtttc ctccatatcg ctagttgcgg ttattagttt
     2941 tgcaagcgtt ttcttaaatc atactcataa taggttctat tttacaagta tcacttaaat
     3001 tttgccaaaa aaaaagagcc ctcatacatt tacatatgag gctcctctaa ttaatattta
     3061 attatttatt ttgtattgca tttaacattt cattaactgc taattttcct aactcatttg
     3121 aagctaaagg actgtcacct gttaataatt tacgatcttt taacgttctt cctgtcatat
     3181 cgtcgttaac tacttttaat ccttgtttag ttaataaatc agcaactaac catttcaagc
     3241 gtccaggtaa ataacctatt tcaatatttg caccttcatc taatgagtca gggaagacac
     3301 aaacagagta tccttctaat ggagattttt ctctgttaag ccctgctgaa agtagtgctg
     3361 ctggtccatg acataatgta actataaagc ggtcattgtc taatgcccaa tctaatgttt
     3421 gttgaacgtc ctcactttca gaaataccaa caactgcagc atgtccgcct gggataaaga
     3481 cagataaata gtctgaatca ggtcctaatt catttttaat cacatctgct aatttttttg
     3541 gctgttttaa tttttctttc aatttattat aagtacttat aactgcctcg tcttcagttg
     3601 gcatagccca taattctaat ttaactggat aaccagataa tgtcgcaaca tcaacgtcaa
     3661 aacctgcttc cattaaatga tgtaaaggta ataacatttc aacaggatga ttacccgtag
     3721 agaacatttt tccattttcc aataatacat atcgctcttc agcagcaatc atcaatactt
     3781 tccatttacc atctttatag gcacctttgt gttcaacacc atcaaaatct gttttaggtg
     3841 ctgtatattg actaagggaa tatggtgatg ggaaaaatgc gttatcttct gctttatctg
     3901 gcgttggttg cttacttaat tcatttacat cttgtgacat agtttattcc tactttctct
     3961 atatagttac tactagtata gacatatact acacactata aacatgtatt actattaaaa
     4021 tgctcattat caaaaataat caaaatgaat ttctttgaaa aaaatacaac taaattctac
     4081 aagatgcttt taaaattcaa acttcaattt tggggagcac taaacattga tatacgaact
     4141 tactgttaat aaactaaaca ttaaaaaaag cccacgacaa tatttgccgt gggaataaat
     4201 aaatattaaa tcaacttcat ttcttttcct actttttcat aagccgcaat tgcttcatct
     4261 aacatgtctt ttgtatgtgc agctgtaggc atatttctta cacgtcctgt acctcttggt
     4321 actgttggga aaacgataga tttcacatag acaccttcgt cttttaaacg cttactaaat
     4381 tcttgagttg ttttttcatc accaataatt actggtgtaa ttggagtttc tgactcacct
     4441 gtatcatatc ctaattttga caatccattt tttaaatatt gtgcattgtt ccataattta
     4501 tcatgtaatt cagttgaatc cattaacttt ttaactgctt cagttattgc tttggtatcc
     4561 ccaggtgcta atgatgtaga gaataagaat ggtcgtgatt gtgcttttaa ccaatctatt
     4621 aactcttttg tacctgctac ataaccgcca acgacaccaa ttgctttaga aagcgtacct
     4681 atttggaaat cgattttatc ttgtaaacca aaatgtttaa ccgtaccagc gcctttaccc
     4741 ataacacctg aaccatgagc gtcgtcaaca taagttaata aaccaaattc ttctgcaatt
     4801 tctacaattt caggtaattt agccacatca ccatccatac taaaaacgcc atcagtgata
     4861 tacatcactt tattgtattg acctgattca actgcttctt ttgctttcgc acgtaaatca
     4921 tccatgtctg aatggttaac tcgaataatt ttagctttag ataagcgaca tccatcaata
     4981 attgatgcat gattaagctc atctgataaa atagcatcat ttttattcat gacagctgaa
     5041 atagcagcca tattacaatt aaatcctgat tgataagcta ttgcagcttc tgttccttta
     5101 aattttgcta gtgtttcttc taattcgtcg tgtaaatcta atgtaccatt gattgtacga
     5161 acagcgcctg cacctacacc atgtgtatca atagctgctt ttgcagctga tttcaaatct
     5221 tcatttgttg ctagtcctaa ataattattt gaagataagt taatgtatga tttcccattg
     5281 attttgattt ctggtccgtt tgcaccttca attgtatcta tttcattata caaaccattt
     5341 tcttttagat aatttatatt ttcctctaaa aactcatgta atgattgaac cacagtatat
     5401 aatccccctt aagtattgtt tggtaatacc tttatcatac agtaattcta cataacttga
     5461 aagccttatc attcatagat aaaatatctc aaagcaatca tttagtacga taatttcata
     5521 aaatgttata ctataaacat tattttagaa agtagtgata agggtgttag attggttcca
     5581 actagcaaat aataaagaaa ataaaacaat tcaactcaga cgttatttac accaatatcc
     5641 tgagttatct tttgaagaat ttcaaacaca tgattatatt gttaaccaat taagccagtt
     5701 atcttgtgat attgaaactc ctatcggccg caatggtatc aaagccacat ttaaaggctt
     5761 gggcacagga cctaccattg cattaagagc tgactttgat gcgttacctg tcgaggaatt
     5821 aaacgatgtg ccttataagt ctaaaaatcc aggatgtatg catgcgtgtg ggcatgatgg
     5881 tcatacagca attttactta ctgtagcaga gattttagat gaacataaac atttattaga
     5941 aggtaatgtt gtattaatat tccagtacgg tgaagaaatc atgccaggtg gttctcaaga
     6001 aatgatagat gctggatgtc tggaaaatgt agatagaatt tacggtacac atttatggag
     6061 tggttatcct actggaacta ttcattcacg tgcgggggca atcatggcct ctccagatga
     6121 atttagcgtc acatttaaag gtcgtggtgg tcatggtgcc aaacctcatg aaaccattga
     6181 cccaatagta atcatggcag agttcatttt aagtgcacaa aaaataattt cacgtacgat
     6241 cgatccagtt aaacaagctg tactttcatt tggaatgatt caagcaggca ccacagatag
     6301 cgttattcct gatcaagcat tttgtaaagg tacagttaga actttcgatt cagatataca
     6361 gaatcatgta atggacaaaa tggataagct tctacaaggt ttagcaatag caaatgatat
     6421 taactatgat ttgaattata ttaaagggta tttgcctgtc cataataacg agaaagcata
     6481 tcaagttatt aaagaagcaa caaatgactt acatgtacgc tttaatgaat cagatttaat
     6541 gatgattgga gaagattttt cacattattt gaaagtacga ccaggtgcat tctttctgac
     6601 tggttgtggt aatgaaagta aaggtattac agcgccacac cataatccca aatttgatat
     6661 tgatgaaaaa tcattaaaat acgctgtagc tgtattttta aaaatcatag aattagagca
     6721 agtatttaaa acaaattaag catttaaatt aattgaaaga tatgaatctg agacataaac
     6781 taatgaaatg ctcccttcaa agtagacatt gaaaaaatga aaactttgaa gggagcattt
     6841 tacaatgtcc aagactcaac aattttattt aaacagtatt atcagttata agttttatta
     6901 gtcagtcttt catcaattat tgttcttcta aatcgaatag ccattacaaa atttacacat
     6961 taaaaaaaga cccctcgatt gagggatctt ctttttatat aaaatctaag aaattagaaa
     7021 ttatttaatg atttcagtaa caacgcctga tcctacagta cgtccacctt cacggattga
     7081 gaaacgagta ccgtcttcaa tcgcgattgg agcgattaat tctactgtca tttcaacgtt
     7141 atcaccaggc attaccattt cagtaccttc tggtaagtga acaacaccag ttacgtcagt
     7201 agtacggaaa tagaattgtg gacgatagtt tgagaagaat ggagtgtgac gtccaccttc
     7261 gtcttttgat aatacgtata cttctgcttt gaattcagta tgtggtgtaa ttgaaccagg
     7321 agcagctaat acttgaccac gttgtacgtc ttcacgagca acaccacgta ataatgcacc
     7381 aatgttgtca ccagcttcag cgtagtctaa taatttacgg aacatttcaa cacctgtaac
     7441 agttgtttta gatgtgtcat gtaaaccgat gatttcaact tcttcaccaa ctttgatttg
     7501 accacgttca acacggcctg tagcaacagt accacgacca gtgattgaga atacgtcctc
     7561 aactggcatc atgaatggtt tgtcagaatc acgttctgga gttggaatgt aagtatctac
     7621 agcttccatt aattctaaga ttttttcttc gtattgagca tcgccttcta aagcttttaa
     7681 tgctgaacca gcgattacag gtacatcgtc acctgggaag tcatattcgc ttaataagtc
     7741 acgaacttcc atttctacta attctaataa ttcttcatcg tcaaccatgt caactttgtt
     7801 taagaatact actaatgctg gtacaccaac gttacgtgat aaaagaatgt gttcacgagt
     7861 ttgtggcatt ggaccgtcag cagcagatac tactaagata ccgccgtcca tttgagcagc
     7921 accagtgatc atgtttttaa cgtagtcagc gtgtcctggg cagtcaacgt gagcgtagtg
     7981 acgtttgtca gtttggtact caatgtgaga agtattgatt gtgataccac gttctttttc
     8041 ttctggagcg ttgtcaatca tgtcatatga ttgtgcaact gagtcaccat tttttgctaa
     8101 tacagtagcg attgctgctg ttaatgttgt tttaccatgg tcaacgtgac cgatagtacc
     8161 gatattggca tgttctttag aacgatcgaa tttttctttt gccattataa aatctctcct
     8221 attctaatta aaaataatat ttaaatttat ctctcatgat agtttctcac catcatgaga
     8281 aattatttat agcgatgcaa aaagaatttt acaattcttt tataatgcat tcaccccaaa
     8341 aaatcaaact ataaagatga tagcttacat ttaagctcac cttgtatttt aacctaggct
     8401 agctagtcaa aacaagttat attattcacc tttatttttc ttgataatat cttcagcgat
     8461 tgattttgga acttcagcat agtgatcgaa gtacatagtg taagtaccgc gaccttgagt
     8521 gtttgaacgt aatgatgttg cataaccgaa catttctgaa agtggtacat aagcattaac
     8581 aacttgtgca ttaccacgag gttccatacc atcaacacgt ccacgacgag atgttacgtc
     8641 acccatgata tcacccatgt actcttcagg catttcaata gttactttca tcattggttc
     8701 taagattaca ggatcacatt ttttagcagc ttctttaagt gctaatgatg cagcaatttt
     8761 gaaggccatt tcagatgaat cgacatcatg gtatgaacca tcatataatt tagctttaac
     8821 atcaattaaa ggataacctg ctaaaacacc attttccata gcatctttaa gaccagcttc
     8881 tactgatgga atgtattcac gaggaactac accaccaacg atagcgtttt cgaattcgaa
     8941 acctgcgcct gtttcgtttg gtgtgaattc aatgtgaaca tcaccgtatt gaccacgacc
     9001 accagattga cgagagaatt taccttgaac ttgtgcagat gatttgaatg tttcacgata
     9061 tgaaaccatt ggagcaccta cgttacattc aacgttgaat tctttcttca tacggtctac
     9121 taagatgtct aagtgaagct cacccatacc accgatgata acttgtccag tttcttcgtc
     9181 agtgtgtgca tggaatgttg ggtcttcttc ttgtaattta actaaagctt gagtcatttt
     9241 atcttggtca gctttagatt ttggctctac tgataagtga ataactggct ctgggaattc
     9301 cattgattcc aagataatgt catttttctc accacataaa gtatcaccag tacctgtatc
     9361 tttaagacct accgcagcag cgatatctcc agagtataca gtatcgattt cttgacgtga
     9421 gttagcgtgc atttgtaata aacgacctac acgttcacgt ttacctttag tagagttctt
     9481 aacgtatgaa ccagatgtca ttgtacctga atacacacgg aagaatgtta atttaccaac
     9541 ataagggtca gtcataactt tgaacgctaa tgcagcgaat tcagctgaat cgtctgcttt
     9601 cgcgattact tcttcttcag ggttgctagc acggtgacca ataattggtt taacgtctag
     9661 tggtgaaggt aagtaatcaa ttacagcgtc aagcattaat tgaacacctt tgtttttgaa
     9721 agctgtacca caaagtactg ggtagaattc tacgttagta gtagcttggc ggatagcttc
     9781 ttttaattca gaaactgaaa tttcttcgtc accaagatat ttttccatta attcgtcgct
     9841 agtttctgca actgcttcga ttaagctagc acgagcttct tcagctctat ctaagtggtc
     9901 ttcgggaatt tcaatttctt caatttcagt acctaaatca tttgtatatt tgaaacattt
     9961 catttcaact aagtcaatga ttgcttcgaa ttcgtcttcc gcaccaattg gtaattggat
    10021 tggagcagcg ttagcttgta aacgatcatg taatgtactt acagagtatt cgaagttagc
    10081 acctaatttg tccattttgt ttacaaatac gatacgtgga acaccataag ttgtagcctg
    10141 acgccaaact gtttcagttt gaggttcaac acctgattgt gcatcaagta ctgtaactgc
    10201 tccgtcaagt acacgtaatg aacgttcaac ttctacagtg aagtctacgt gtccaggtgt
    10261 atcgataatg tttacacggt gaccttccca agctgctgtt gttgcagcag atgtgatagt
    10321 aataccacgg tcttgttctt gctccatcca gtccatttgt gaagcacctt cgtgtgtttc
    10381 accaattttg tggatacggc cagtgtaata aagaatacgt tcagtcgtag tcgttttacc
    10441 agcatcaatg tgagccatga taccgatatt acgagttttt tctaatgaaa attctctagc
    10501 catgtatttt ttctccttcc agtttatatt actgaataaa tacgatagat atggcgatgc
    10561 cctaagcatg aacgcttaat gaaaattcat taatatagaa cacactcagg gtaaaagctt
    10621 ttatcttacc aacggtagtg agcaaatgct ttgtttgctt cagccatttt gtgagtgtcc
    10681 tcacgtttct taacggcacc acctgtatta tttgctgcat ctaaaatttc gttagctaaa
    10741 cgatcttcca tcgttttttc accacgaaga cgtgcatagt taactaacca acgtaaacct
    10801 aaagtagtac gacgctctgg acgaacttct actggtactt gatagttaga accacctacg
    10861 cgacgagctt taacttctaa tactggcata atgttgttga ttgcttcttc gaatacttct
    10921 aatgcatcac gaccactgcg ttgttcaact aggtcgaatg ctgaataaag aattctttgt
    10981 gctgttccac gtttaccatc taacataatt ttgttaatta atttagttac taacttagag
    11041 ttatgaattg gatctggtaa tacgtctctt ttaggtactg atcctttacg aggcataatg
    11101 taaatcctcc cttccttata ttaaatattt taagtttaag atttaattaa aaactaaatt
    11161 cttagttttt aggtttctta gttccgtata atgaacgacc ttgtctacgt ccgtcaacac
    11221 ctgaagtatc aagtgctcca cgtacaatat ggtaacgcac acctggtaag tcttttacac
    11281 gtccaccacg tacaagtaca acactgtgtt cttgtaagtt atgtccgata ccagggatgt
    11341 atgcgttaat ttcgatgttg tttgataaac gcacacgtgc atatttacgt aacgctgagt
    11401 taggtttttt aggtgtcatt gtacctacac gagtacatac accacgtttt tgtggtgagt
    11461 ttaagtcagt aaattttttc tttttactgt tgaaaccttt atttaaagct ggagaatctg
    11521 attttttgat tttgctttgt cttggtttac gtactaattg gttaatagtt ggcatgtgat
    11581 atgtcctcct ctcttcactt tttaatccca cacatccagg tggttcattt ttaggttaaa
    11641 taaaatttag taagtaaaac acttactaat tctcatttaa tcaatgctac tattgtcgca
    11701 ttgacgttaa tacctacatg tttacccaaa gcatgtttgc ttttgaaaaa agatacaggt
    11761 atatttttct gattgatttg gcttaacacg cgagtcatta aatatacttc aacgtcttca
    11821 gcaataatca aagatgtaac ttgatctttc tttaacgctt taagcgtttc tttaagacca
    11881 actacaaaat gttgtttgtt aaagcgtgca actttttcct tagacaataa tttatcctcc
    11941 aaagttctgc ttgcatcaac ctttaatata ttaacattga acaagagaat tcgtcaacat
    12001 taaatcaaat aatttacata aaaataacaa gaggctaaag cattagcctc tgttatatac
    12061 ttgttattcc gttacttcag tttgagattc aacttctgca actggtttag ctgttttttc
    12121 gtattttacg tcgctataac gtctcatacc agtaccagct ggaattaact taccaataat
    12181 tacgttttct ttaagaccta ataagtcatc acgcttacct ttaattgctg catctgtaag
    12241 aactcttgtt gtttcttgga atgatgctgc agataagaaa ctttctgttt caagtgatgc
    12301 tttagtaata ccaagtaata ctggtttagc tgttgcagga cgcttacggt gtttaaatgc
    12361 ttctctattt gcatctgtaa agttatgaat atcaactaat gaacctggta ataacttcgt
    12421 atcacctgct tcgataattc taactttacg taacatttgt cgaaccataa cctcaacgtg
    12481 tttatcgtcg atttctacac cttgcatacg gtaaactttt tgtacttctt ttaataagta
    12541 gctttcagtc gcgtttaatc cagcaacaga taagtaattc ttaggttcaa tagaaccttc
    12601 agttaatact tcaccacgtt gaactggttg accgatttct acaataattc ttgaagtacc
    12661 tgaagcaagg tatgatcttg tttcattagc acctttaaca acaatttctt gttgtctatc
    12721 ttttgctaat ttaatatctt ctacgacacc ttcgatttcc gtaattaccg cttgaccttt
    12781 agggttacgt gcttcgaaaa tctcttgaat acgaggaaga ccttgtgtga tatcgctacc
    12841 tgctacccca cctgtatgga atgtacgcat tgtaagctgt gtacctggtt caccgataga
    12901 ttgggctgca attgtaccaa ctgcttcacc aacttcaact ttttcaccag tagcaaggtt
    12961 tttaccgtaa catttttcac aaacaccatg tcgtgcgtta caagtaaatg ctgagcgaat
    13021 atacatttgt tcaataccag catctgtaat tttcttagca atttcaggtg taattaattc
    13081 atcaggacga atgattattt catcagtttc aggatgatga attgtttctt tagaataacg
    13141 accttcaata cgttcgataa atggttcaat catttctgta ccttctttaa tatcagaaac
    13201 taataaacct ctatcagtac cacagtcttc ttcacgaaca ataacatctt gtgccacgtc
    13261 aacaagacga cgagtaagat atcctgagtc agctgtttta agtgctgtat cggcaagacc
    13321 tttacgtgca ccgtgagttg agatgaagta ttcaagtact gttaaacctt cacggaatga
    13381 agatgtgatt ggtaattcga taatcttacc agatggtgcg gccatcaatc cacgcatacc
    13441 tgctaactgt gtaaagttag atgcgttacc acgggcacct gaatcactca tcatgaagat
    13501 tgggttagtt ttatcaagtg attgcatcaa ttcaccttga atttgatctt ttgcatctgt
    13561 ccaaatttca acaactgcat tatatctttc ttcttcagtg attaaaccac ggttgaattg
    13621 ttttgtaatt ctgtcgacta atttttcatg ctcatcaagt atttgttgct tatcaggtaa
    13681 tactacgata tcagcaacac ctactgtaat accagcttta gatgagaatt tgaatcctaa
    13741 gtctttcata cggtctaaca tcattgatgt atcagtgatg ctaaatctgt tgaatacttc
    13801 tgcaataata ttacctaaga attttttgtt gaaaggttca attaattctt cattttcaaa
    13861 gtattctttt aatccacctt cacctaaagt tgtaggatcg atgaaatatc tgtttggtgt
    13921 ctttctttct aagttttctt gcgtaggttc attaatataa gcaaatgaat ctggaatgat
    13981 ttcattgaat ataattttac ctactgacgt agcaagaatc tttttgtttt gttcttcagt
    14041 aaatgttgga ttattgaacg aacttgcatg tacaccaatt ctagtgtgta aatgtacaaa
    14101 gccatttgca tatgctttta atacttcatt tgtattatta aagattgcgc ctgtatttac
    14161 tgcatctttt ctttctaaag taaggtaata gttaccaagt accatatctt gtgatggtgt
    14221 aactacaggt ttaccatctt tagggttcaa gatgttttgt gctgctaaca tcaacattct
    14281 tgcttcagct tgtgcctctt ttgataaagg aacgtgaacc gccatttggt caccgtcaaa
    14341 gtcagcgtta taagctgttg ttacaagtgg atgtagacga atcgcacgac cttcaactaa
    14401 agttggttca aatgcttgaa taccaagtct atgaagtgtt ggtgcacggt taagtaatac
    14461 aggatgttct ctaattactt cttccaatac gtcccaaact tcatcatcca tacgttcgat
    14521 tttactcttc gcatttttaa tgttagttgc aatttcacgt tgaactaatt ctttcattac
    14581 gaatggttta aatagttcaa gtgccatttc ttttggtaaa ccacattggt acattttcaa
    14641 gcttggacct actgcaataa ctgaacgtcc tgaatagtca acacgtttac caagtaagtt
    14701 ttgacggaaa cgaccttgtt taccttttaa catatgagat aaagatttta atggacggtt
    14761 acctgggcca gtaactggac gaccacgacg accattatca attaaagcgt caacggcttc
    14821 ttgtaacata cgtttttcgt tttgaacgat gataccaggt gcacctaaat ctaataaacg
    14881 tttcaaacga ttatttcgat taattacacg acggtataag tcgtttaagt cacttgttgc
    14941 aaatcgtcca ccatctaatt gaaccattgg acgaatttct ggtgggatga ttggaagtac
    15001 atctaaaatc atccatgaag gtttgttacc tgaattacgg aatgattcaa caacttctaa
    15061 acgtttaatt gcacgagtaa gtctttgacc agtagctgat tccaactcat cgcgtaacaa
    15121 tttaagttct tcgtcaagat caatctcttc aagtaaatct ttaatacctt ctgcacccat
    15181 ttttgcaacg aattgacctg ggtatttatc ataataatct ctgaattcag cttcagataa
    15241 taaagttttc ttttctaaac cagttggacc tggatctaca acaacataag aagcaaagta
    15301 aataacttct tctaatgctc ttggtgacat gtcaagtaat aatcccatac gacttggtat
    15361 acctttgaaa taccaaatgt gagaaactgg agcagcaagt tcaatgtgac ccattctttc
    15421 acgacgtact ttagatttag ttacttcaac tccacatctg tcacagacca tgcctttgta
    15481 gcgaacacgt ttgtatttac cacaactaca ttcccagtct tttgtaggtc cgaaaattct
    15541 ttcacagaat agaccatctt tttcaggttt taatgtacgg tagttgattg tttcaggttt
    15601 tttaacttca ccaaaagacc aagaacggat tttttcaggt gaagccaatc ctattttcat
    15661 ataatggaaa ttatttacat caatcaagga gcctacctcc ttcaatttag attagctgtg
    15721 ctatttgatt gatttgcatt tatttaaaat ggcttaaagt actaaactga atcatcaata
    15781 aaattcagct tagtatcttt ttgcctgttt tgtaaattgc gtattaatca gtaacttctt
    15841 tttgtgtttc aggagcatca ttttgttgta aatctacttt gcgttctaca acatcatcgt
    15901 catcaacgtc tgtcatttcg atttcattat cttgctcatc cataactttt acatctaaac
    15961 ctaaactttg taattctttc atcaatactc ggaatgattc tggaacactt ggtctagaga
    16021 tgttttcacc tttaacaata gcctcgtatg ttttcacacg tcctactgta tcatcggatt
    16081 tgtaagttaa gatttcttgt aatgtgtatg cagcaccata tgcttcaagt gcccatacct
    16141 ccatctcacc aaaacgttgt ccaccgaatt gcgctttacc gccaagtggt tgttgtgtaa
    16201 caagtgaata tggtcctgtt gaacgcgcat gtaatttatc atcaaccatg tgcgcaagtt
    16261 tcaacatgta cattacacct actgaaatac ggttatcgaa tggttcacct gtacgtccat
    16321 cataaagtac agttttacca tcacgagcca taccagcttc ttcaattgtt gaccatacat
    16381 cgtcatcgtt tgcaccgtca aatactggtg atgcaacgtg aataccaaga tttttagcag
    16441 ccatacctaa gtgtagctct aatacttgtc cgatgttcat acgagatggt acaccaagag
    16501 gatttaacat gatatcgatc ggacgtccat ctggtaagta aggcatatct tcttcaggaa
    16561 caatcttaga aatgacacct ttgttaccat gtcgaccaca catcttatca ccaacatgaa
    16621 ttttacgttt ttgaacgata tatacacgta ctaattggtt tacaccaggt gataatgtat
    16681 cgtcgccttc ttcacgattg aatactttta catcaagaac gataccgcca gcgccgtgag
    16741 gtacacgtaa tgaagtatct ctaacttcac gtgctttttc accaaagatt gcatgtaaca
    16801 atctttcttc ggcagttaac tcagttacac ctttaggcgt tactttacca actaaaatat
    16861 ctccatcttt tacttctgca ccaatataaa cgataccacg atcgtctaag ttcttaagtg
    16921 cactttcaga aacattagga atatctcttg tgatttcttc aggtcctaac ttagtatcac
    16981 gtgcttctga ttcatactct tcaatatgaa tagaagtata cacgtcatct ttcacaagtc
    17041 tttcactcat gataacggca tcctcatagt tgtaaccgtc ccaagtcatg aaaccaacta
    17101 ctacgtttct acctaatgcc atttctccta attccataga tggtccatct gctaaaatct
    17161 cgttatactc aacaacatct ccaactgcaa cgattggacg ttggttgtaa catgtacctg
    17221 agtttgaacg tttaaattta gctaatggat agcgatctaa ttcaccttca tgctcaacgc
    17281 cgttctcttc aactagacga cgaacaagaa tttcattaga ttcaacatgt tcaacacgac
    17341 ctctgtgctt agctgtaata gccgcaccag aatcacgtgc tgcaacgtgt tccatacctg
    17401 taccaacaaa tggtgcttct ggattcatca aaggcactgc ttgacgttgc atgttcgcac
    17461 ccatcaatgc acggtttgag tcatcatttt ctaagaatgg aatacatgct gtcgctgctg
    17521 aaacaacttg cttcggcgat acatccatat aatccatttt ttctttagcc ataactgtat
    17581 tgttaccacg gaaacgacat acaacttcat catccatgaa acgaccattt tcatctaatt
    17641 tagagtttgc ttgtgctaca acatagctat cttcttcgtc agctgttaaa tagtcaattt
    17701 gatcagtgat agcatgtgta tctaaatcaa ctttacgata tggtgtttca ataaagccga
    17761 attcatttac acgtgcataa cttgataatg agttaatcaa tccaatgttt ggtccctcag
    17821 gtgtttcaat tggacacata cggccatagt gagagtagtg aacgtcacgt acttccattt
    17881 gagcacgttc acgtgttaaa ccaccaggtc ctaatgctga tagacgacgt ttatgcgtta
    17941 actcagctaa tgggtttgct tggtccatga attgtgataa ttgagagcta ccaaagaatt
    18001 ctttaataga tgcaataaca ggtcgaatat taattaattg ttgaggtgtg atagactcag
    18061 tatcttgaat tgacattctt tcacgtacaa ctctttccat tcttgataaa ccgatacgga
    18121 attggttttg tagtaattca cctacagaac gtaaacgacg gttacctaaa tggtcaatat
    18181 catctgtata tccaataccg cttaataagt taaagaagta actcattgaa gcaatgatat
    18241 ctgctggtgt aatgcattta acttctgagt cagggaaagc attaccaatt acagttgtcg
    18301 tacgaccttc atcatcgtta ggaacatata ctttaattga ttgaatttct actggctcgt
    18361 ctataacgct accatgcaat tcaaacactt cgctgtttgc atttgattca agtacatcca
    18421 tgatttcgtc gattttacga cgatcaagca ctgtaccttc ttcaactaca atttcaccag
    18481 tttcagtatt tacaattggc tcagctaatt tttgattaaa taaacgatgt tttaaatgta
    18541 attttttgtt tgttttataa cgacccacgc ttgctaagtc atagcgtttt ggatcaaaga
    18601 aacgtgaata caatagactt ttagcatttt caacagttgg tggttcacct ggacgtaaac
    18661 gttcatagat ttctaataac gcttgttcag tgttttcagt gccgtctttc tctaaagtat
    18721 tacgtaaata ttcattgtca cctaaaaggt caacaatttc ttggtcgctt gagaaaccta
    18781 atgcacgtaa caatactgtt aatggtagtt tacgtgttct atcaatacgt acgtatacaa
    18841 catctttagc atctgtttca tattctaacc atgcaccacg gtttggaata attgttgcat
    18901 catagttttc acgaccattt ttgtcgattt tttcattgaa ataaacggat ggtgaacgaa
    18961 ctaattgaga tacgattaca cgttctgcac cattgataac gaacgtacct gtatcagtca
    19021 ttaatgggaa atcacccata aagacttctt gttctttaac ttctcctgtt tctttaatga
    19081 ttagacgcac ttttacacga agaggtgcag cataagtagc gtcacggttt ttagattctt
    19141 ctaaatcata ttttggttct cctaaacggt aatccacaaa ctctaatgac aaattaccag
    19201 taaaatcttc aattggagaa atgtctctaa acatttcgat taaaccttct cttaggaacc
    19261 actcgtaaga tttagtttga atttctatta agtttggtaa ttctaatact tctgaaattc
    19321 tcgcgtagtt tctacgttta cgatgtcttc catattggac aacttgacct gccaaacaga
    19381 ttcacccctc aaaaattatg tgatccgctt ttagctaaat accataagca tattaataaa
    19441 aagacaaaaa gaaaacggca acactaatga tgacaccatt ttcgattcta tatttattct
    19501 taattaactt atctatttaa cgtaaaagta cacacttatt gaacataaat ttttacatta
    19561 tataactata acagaatatc catttcattt caagctttta tacttctcag aatgtaatat
    19621 cctttatctt tatttaccac ttctacattt ccaaaaagtt cattcattct tttctttgca
    19681 gatggcatac cttgcttctt ctgaattaca acgaatagtt caccgttcga gtctaatcta
    19741 tgtaatgctt gctcgaatat acgatgcacg gtttctttcc ctgctcttat tggtggattg
    19801 gttaaaataa aatcaaaact tttgtcttcc acagcagaca aagcatcact ttcctttacg
    19861 atcacattat caataccatt taattttttg tttttttcaa ctaaggctag cgctctgtga
    19921 ttaacatcta gcattgtaat tgaatgatgt ggtgatactt tagcaatcat caaaccaatt
    19981 ggtccgtaac cacaaccaac atcggcaatt cgcttacttg gaccaggtgg atgcgctttt
    20041 aaaaaagttt gaacaagaac atctgaacca taatctactt tatctttcga aaacactccg
    20101 ttatcagtta ttaaatcaat tttatgatgg tttaattgat attgaatacg ttgttcattg
    20161 ctaattacac ttggatcttc atcgtaataa tgactcatta ttccacctca tcttcacttt
    20221 gtaactcgtc attataatta taacatttca ctcagtcttt aaatgaataa aaccccgtta
    20281 tcaaaataac ggggttttaa cttctcaaag aatcattata ttatagcaat acttgctata
    20341 aaactttgat tattaagttt aagatacttg aattatttta attctacagt agctccaact
    20401 tcttctaatt gttctttaag tttttcagct tcttctttag gtaaagcttc tttgattact
    20461 ttaggagctc cgtctactaa ttctttagca tcttttaatc ctaaaccagt tgcttcttta
    20521 acagctttaa caactttgat tttagatgaa ccagctgaag ttaactcaac gtcaaattca
    20581 gttttttctg ctgcagcgtc agcgccacca gctgcacctg ctactgctac tggagcagct
    20641 gcagttacac caaattcttc ttcaattgct tttactaagt cgtttaattc taatactgac
    20701 atttctttaa tcgcttcaat gatttgttca tgattagcca ttttataatt cctccattat
    20761 ttttaatttt tttacgctaa aaattattca gcgttttctt ctttttgttc tccaatagct
    20821 ttaaccgcat aagcgaagtt gcgtacagga gcttgtaata ctgataaaag catagataca
    20881 agaccatcgt gtgaaggtaa tgaaccaaca gttttaactt cttctgctgt aataacattg
    20941 ccttccataa cgcctgattt aatttctaat gcttcatgat ctttagcaaa tccagaaatt
    21001 actttcgctg cagctacagc atcttcactt gaagttgcaa tagcagtagg acctgttaag
    21061 aattcatcta agccttcgat accagctttt tcagctgcac gacgtaccat agtgtttttg
    21121 tatactttat actcaacacc agcttcacgt aattgtgaac gtaagtcagt aacttcagct
    21181 actgttaatc cacggtagtc aacgattact gttgaaactg aatttgatag tacctcagca
    21241 atttcatcaa ctagttgttt tttagcttca atgatagcag acattcagac acctccattt
    21301 aaatttttgg tgcttttttt aaaattaata aaaaaagcac tttctaccca cggcaaaaag
    21361 tgcttgaaag atcatagatc atcacgttca agtcaatttt agcctcggca ggataaattt
    21421 taagttataa ataactccta ctgtcttagg taaaataatc acaataagta atataacttg
    21481 gaaattactt attgtcaata tatttttaca gactgaaaga tatttctttc agcctgtaat
    21541 tgtttatatc atttattatt tgaaacttgc agtatcaatt ttaactccag gacccattgt
    21601 tgtagttaca gcaacagatt tgaagtatgt acctttagca gatgatggtt tagctttagc
    21661 taatacatct tgtaaagtat tgaagttttc aattaattgt tcatcagtaa atgaaacttt
    21721 accaattgat gcatgtacga taccagcttt ttcagcacga tattctactt taccagcttt
    21781 gatttcttca acagcttttt taacatccat tgttacagtt ccagttttag ggtttggcat
    21841 taaacctttt ggtcctaata cacgacctaa tttaccaact tcacccatca tgtctggtgt
    21901 agcaactact acatcgaagt cgaaccaacc ttgttggatt ttttgaacgt attctgcttc
    21961 acctacatag tcagcacctg ctgcttcagc ttcagcaatt ttgtcacctt tagcgaatac
    22021 taatacactt tgtgatttac cagttccgtt tggtaataca actgcaccac ggatttgttg
    22081 gtcattttta cgtgtatcaa ttcctaaacg gaatgcaact tcaacagaag cgtcaaagtt
    22141 agcaatgctt gtttctttag ctaatttaat tgcttcttca acactgtagt gctgagtacg
    22201 gtcaacttta ctagctgctt cttgatactt tttacctttt ttagccattt atagttcctc
    22261 ctttagtggt tttagcggaa tttcctccca cattacttgc tatatgcaag ttaagagcag
    22321 acaacagaag aattaatctt atgaataaat caatcattaa tctagtcatt cagattatat
    22381 ttataattca tcattattat ctctgttatc tgcttcatcg tttaaatcat gtaatcaaat
    22441 ttaatattcg taaaattatt ctacaacgat acccatacta cgtgcagtac cttcgataat
    22501 acgcatagct gcttcttcgt cagcagcgtt taagtcttgc attttgctgt tagcaatttc
    22561 gcgtacttga tctttagtta ctgtagcaac tttagttttg tttggttcgc ctgaaccttt
    22621 ttcaatacca gctgcttttt taagtaatac tggagccggt ggagtttttg taataaatgt
    22681 aaatgaacga tcttcataaa cactgatttc taccggaata attaaacctg cttgatcttg
    22741 agtacgtgca ttgaactctt tacagaatcc catgatgttc acacctgctt gacctaatgc
    22801 tggaccaact ggtggtgctg gattcgcttt acctgcagga atttgtaatt taacaacttt
    22861 atctactttt ttagccacga tgtgcacctc cttgatatcg tgatgtggtc acagggctca
    22921 gttttgccct cccactctta acatttcgtg acgaaatggg cgctctaaaa gcgcgaccac
    22981 ataattttaa cacttttcta attaaaaaat caatactctt ttttatcttt tggttagttt
    23041 aataacttta attgttaatt aaagcttttc tatttgatcg aattcaactt ctactggtgt
    23101 ttctcggcca aacatatcta ctaatactgt tagcttaaac ttatctgttt caatttcttg
    23161 aacttcacca acttgattcg caaatggacc tgatttaata cgaacttgct cgccaacttc
    23221 gagttcaaca tcgatagtct tttctttaag acccatttgt tttaagatga agcgaacttc
    23281 ttctggtaac aatggatttg gcttagaccc tgcacctgca gaacctacaa aaccagtaac
    23341 gcctggtgta tttcttacca cataccatga ttcatctgtc atgattaatt ccactaaaac
    23401 atatccaggg aatgtttttt taacagtcgt tttagctttg ccatctttta cttgagtttc
    23461 ttcttcttcc ggtatgacta ctctaaagat ttgttcagtc atattcatag attctactct
    23521 tttttctaaa ttctttttaa ctttattttc atatccagaa tatgtatgca ctgcatacca
    23581 acgctttgcg ccaacttctt cagacatgtc ttcactcctc taacgaaata ataaattttt
    23641 caatgctgta attcctaaat ctaaggcata gaaaaagact aagaagaata taacagtaga
    23701 aactacaatt acagtatatt taaatagctc ttctttcgtc ggccaacttg ttttttccat
    23761 ttcagactta acgcctttaa agaaactttc ttttttagcc atctgctcaa cctccaaatc
    23821 ctttgaacat attatcaaag tattatttcg aatgtattta tttcgattct ttatgaattg
    23881 tgtgcgcgtt acattttgga caatatttct ttaaggttaa tcttgttgcc gagccttctt
    23941 gcttaggaac attataattt ctattgccac aagcttcaca atttaaaggt atttttctca
    24001 caataattca cctttattca ttataatacc agtatactat acataaaatt tataacaaat
    24061 gtcaaaccat cagtaagtaa cacgttataa ttcaattcac gtcctaaggc gcttttcaaa
    24121 tcattttgaa ataacgtttt attttatttt tacatcgttg tatggcatta taaatcactt
    24181 tctcttttac atgcatcaat tgagcaattt ctctcggctt atattgttca cacattaaat
    24241 acatgacatc tttttcaaat gcactcaaat acttaaactg acaaatcaac tctttaattg
    24301 ctaatttatt caaataattg ttttcaacat caaaacgctt tattgcacat ggatacgtaa
    24361 ctctatattc attaatcaaa ttatccattc ttttatttgt agctaaatat tttctcaaat
    24421 aatcatactt tacagacgta attaaacatt gaacataatt tgtgaaaggc tgctctccac
    24481 taaaatcaaa tgtttggacc gacctataca tccgtataag tatttcttga tataagtctt
    24541 ctaaatcata atgattaaat ccaaatgttt tgatgcgtcg aataattaat ggttgtagat
    24601 ccattactaa cttttcgaag tctttatcat ttattgcatt gttacgtttg attgtactgt
    24661 cttgagttgt caaatcgtat ttcaaatatt aaactaaccc cttctatcta aaatttaagg
    24721 ttagtttaat attgttacat tcaaaatttc aagatgacgg aaatgtcatt tcttatgatg
    24781 tcctcttcgt attttttcaa attctgcaag gatttcagaa gataacggaa ttcgagttct
    24841 tggcttgttt tcacttatat catctaatga tttactcaca tcaatttcat tttcttttaa
    24901 atctctccac atttcgcgag atgatattct atatgcacct gatccaaaga tagcatgttg
    24961 ctcactcata tcacttgtta caactgtaat atgcttagta tgcttgtcat aaagttcata
    25021 aacataacgt tctatgaaac tatcagctgt ttctttttcc ttggtaaaaa tcgttttaac
    25081 gccatgatac atgtattctc tttcaacacc cgattggtca taagcatcga aaacacaaat
    25141 aatttcatct gaaataactg cattataatt tgcaattgca tctattaatt gcattctagc
    25201 ttcttctaaa ttctcttttg caatggcgct tagcgttggt gattgtccta tcatattgta
    25261 tccatcaatg attaagtaac gttctttcat tatatttctc caacatcatg tctttttcga
    25321 aatacttcgt acatcattaa acttgctgca accgaagcat tcaaactgtt tacatgtcca
    25381 accattggaa tcttaatata aaaatcgcat ttatcactta ctaggcgact cataccctgt
    25441 ccttcgctac caattacaat agccaatgac atgtccgctt ctagatttct ataatctgtt
    25501 gcattattag cttcagtgcc agctacccaa aagccattat cttttagttc atcgatagtt
    25561 ttagctaaat ttgtcactcg aataactggt acatgttcaa ttgcacctgt tgaggctttt
    25621 gcaactgttt gcgttagtgt aactgaacga cgtttaggaa taataacacc atcaactccc
    25681 gttgcatcgg ctgttcttaa aattgatccc aagttatgtg ggtcttctaa gccgtctaat
    25741 ataagtactg tcaataaacc ttctttttct ttttgctgtt ttaaaaattg atcgaagtca
    25801 gcatattcat atggtgcaat aagcgctgca acaccctgat gtggtgcatt tgctaaaaaa
    25861 tctaatttag attttggtac agtttgaaca atgatttttt gatcttttgc attttttaaa
    25921 atttcattaa tttgttgctt tttaatacct tcttgaatca atatcttatt tatcggatgc
    25981 ccagtaataa tcgcttctct aacagcatgc ctaccaacaa taaccgtatc ttccacatcg
    26041 tcactacctt tcgtttacta ttgttattat tttatttaat aatgcctcta atcgttcttc
    26101 tcttttttct aaataaagaa aacctatcac tgcttctatc gctgaacttt tacgatatgt
    26161 ttgaacatca gtgtttttag ctttagtatg acttttcgcg ttacgccctc gcttcaaaat
    26221 atccatttct tcgtctgtaa accattcttg ctccattaaa tattctaacg tttgcgcctg
    26281 acttttggca gatacatatt ttttagacat ttgatgtagt ttattaggct tacttttaag
    26341 ctttaaaacg atataggtac gtacatattg atctaagact gcgtctccca tatatgctaa
    26401 ggtcaatgga ttcaataatt taatgtgatt atcttgttga ttatccacgt ttaaatctaa
    26461 ccccttgagg tgtgtcttct aatataatgt tttgtgattt cagcatgtct cgaatttcat
    26521 ctgctcgtgc aaaatctttg tttttccttg cttcattacg ctcttcgatt aatttttcaa
    26581 catcttcatc caataattca tctgcatttt tagattttaa cggtacacct aaaacatcgc
    26641 tgaaaatttg ataaactgct ttaaatttat caattacttc tgttgatgtt gtgttctcta
    26701 gtacatattt attcgcaagt tttgctaaat cataccaagc tgtaattgca ttagctgtat
    26761 taaaatcatc attcataact gtttcaaaac gatttaaaat cgcatcaatt tgatcaatat
    26821 atgtctgttg attttcaata ttagtagcaa tttgtgcgcg ctcttcaatt aattgataac
    26881 tattgcgaat acgctctagt ccactacgtg ctgattctac caattctaga ttatagttaa
    26941 ttgggcttct ataatgtacg ctaatcataa agaatcttag tacatctgga tcaacttctt
    27001 taataatatc gtgaactaaa ataaagttgc ctagtgattt actcattttt tcattatcaa
    27061 tattaatgaa accattatgc atccaataat tagcaaatgg cgcatgatta tgtgcttctg
    27121 attgtgctat ttcattttca tgatgtggaa attgtaaatc tgaaccaccc gcatgtatat
    27181 caattgtagg tcctagctca tgaaatgcca ttacagaaca ttctatatgc catcctggtc
    27241 taccttcacc aaatgggcta tcccaactaa tctcgccagg cttcgctttt ttccacaatg
    27301 taaaatcaag tgcatcttct ttatgctctc ctgcatctat acgagcaccc acttttaagt
    27361 catctatgga ttgatgactt aatttaccat aaccttcaaa tttacgtgtt ctaaagtaaa
    27421 catcgccacc actttcatat gcataacctt gatccaccaa atctttaata aattgaataa
    27481 tgtcatccat atggtccatt acccttggat ttgaagtcgc ttttctaaca tttaacgcac
    27541 caacatcttc atgaaaagca gcgatatatt tttctgcaat ttcgggaaca gactgattta
    27601 attcttgaga acgtttaatt aatttatcat ctacgtctgt aaaatttgat acatattcta
    27661 cattatatcc ttggtattca aagtaacgtc tcactacgtc ataattaatt gctggtcttg
    27721 cgttaccaat atgaatgtag ttatatacag taggaccaca tacatacatt tttactttcc
    27781 ctggttctat aggcttgaac acttcttttt gacgtgtaag cgtattatat aatgtaatca
    27841 tcttgaatct ctccattcct agtcttttca agttgtcgtt ctaaatgctt aatttgttca
    27901 taaattggat caggtagatg gcgatgatca aatgtttttc caactcgaac accatcttgc
    27961 ttaacaatat gtcctggtat accaacaacc gttgaataac ttggaactga ttgtaaaaca
    28021 actgaatttg caccaatatt tacatttgaa tttattttaa tatttcctaa aactttcgca
    28081 ccggctgcta ttaaaacatt gtctcctata tctgggtgtc ttttccctct ttctttccct
    28141 gtcccaccaa gtgtcacgcc ttgatagatt gtcacattat caccaattgt acatgtttct
    28201 cctattacaa cgcccatacc atgatctata aatagacgct ttccaatttt agcacctgga
    28261 tggatttcta tacctgtgaa aaatcttgaa atttgagata tcgcgcgtgc tgcaacatat
    28321 tttttttggt tgtataactt atgtgcaatc aaatgactcc aaactgcatg taaacctgca
    28381 tacgttgtaa tgacttctaa tgttgaacgt gccgctggat cctgctcaaa taccattttt
    28441 atatcgtctc tcattctttt taacaagatc atttcctcct caatgattga actacgtaaa
    28501 tacataattg aagtacctgc gaaattaaat atcaaaaaag caccactaac atacaagttg
    28561 tattgttaga ggcgcttccg cacggttcca ctctgaattt agcgaataac attaataata
    28621 ttgcgggcgc ttccaaatta tcaaggaaac taagtcaact taatgctcat cactctcatt
    28681 atatatttaa ttcattttac gaaggtgcat tcattaattt ctacgttgta ctcacagcaa
    28741 ccgtacactc tctgcatcgt ataaatttaa ttactaatcc ttcgttttat ataaaatact
    28801 ttattcatat tgttgtaatt tgttaataca actttatcat atgtggttac gttataaaat
    28861 tcaagtatat actaccttga tcttgtctat ttcattactt atattgtttt aaacggttta
    28921 gcactttttc tttaccaagt acttcaattg tatttggtaa ttcaggacca tgcatttggc
    28981 ctgttacagc aacacgaata ggcataaata attgcttgcc ttttattcct gtttcttttt
    29041 gaacttcttt aattgtcttt ttaatttcag ccgcttcaaa tggttcaagt gcttctaatt
    29101 tactgaataa gtgcgtcatt aactctggta cttgctctcc attaatcact tgttgttctt
    29161 cttcaccaag agctggcatt tctttaaaga acatttctga taaaggtaca atttcaccgg
    29221 cataactcat ttctttttga taaagcgcaa ttaatttgcg tccccaagat aaatcctctt
    29281 ctgacggcac ctcaggaatc aaatttgctt taattaaatg aggtaatgct aattggaata
    29341 ctgtttcagt atctttttgt ttcatatatt ggttattaac ccatgctaat ttttgcttat
    29401 cgaaaaatgc tggtgatttt gacaaacgct tttcatcaaa gattttgata aattcttctt
    29461 tagaaaagat ttcttcttca ccttcaggag accaacctaa taacgcaata aaattaaata
    29521 acgcttcagg taaataacct aagtcacgat attgctcaat aaattgtaaa atttgcccat
    29581 cacgtttact taacttttta cgttcttcat taacaattaa tgacatatga ccaaaacgag
    29641 gtggctccca gccaaatgct tcataaatca taatttgttt aggcgtgttt gaaatatgat
    29701 catcaccacg aattacatct gaaatttgca tgtaatgatc atctatagct actgcaaaat
    29761 tgtacgttgg aatgccatct ttttttacga taacccagtc accaatacca tttgaatcaa
    29821 atgaaatatt tccttttacc atatcatcaa atgaatacgt ttggttttga ggtactcgga
    29881 aacgaattga tggttggcgt ccttctgctt caaattgttg acgttgttct tcagtcaaat
    29941 gcgcatgttg accaccatag cgaggcattt caccacgagc gatttgcgct tcacgttcag
    30001 cttctaattc ttcttctgtc atatagcatt tatatgcttt atcttctgct agtaactgat
    30061 ctattaatgg ttggtagata tgttgacgtt cagattgacg atatggtccg tagccattgt
    30121 ctttatctac agactcatcc caatctaatc ctaaccattt aagattatca aattgtgatg
    30181 tttctccatc ttctaaatta cgttttttat cagtatcttc aattcgaatc acaaaatctc
    30241 cgttgtaatg tttagcatac aagtaattga ataatgctgt tcttgcatta ccaatatgaa
    30301 gatacccagt tggacttggt gcatatctta ctcttatacg atcgctcatt tttttcactc
    30361 ctaaattaaa tatcagattt tcaagttagt tcatataaat tgttcatttg ctatcttcga
    30421 ccgtcataac aaatgtctaa ctcgtcttat tgttaaaacg aaacaatgct ttttaacatg
    30481 accttaaaat aatttcattg tttaatcata acataattcc ctgggtaata tgcttaaatt
    30541 ttaaatagaa agctgttgtt ttttcaacac tttaaaaaag ctatccctaa gaatcaagac
    30601 tgcactacat aaacatttca acgacttaaa aatgaatatt atgtactgtg aaattaaact
    30661 tcaaattaac tattcaaata cgtt
//
LOCUS       DANJFZ010000023        30390 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.023, whole genome shotgun sequence.
ACCESSION   DANJFZ010000023 DANJFZ010000000
VERSION     DANJFZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.023"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..193)
                     /locus_tag="PWN45_000878"
     CDS             complement(<1..193)
                     /locus_tag="PWN45_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622661.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDV"
     gene            complement(772..2049)
                     /locus_tag="PWN45_000879"
     CDS             complement(772..2049)
                     /locus_tag="PWN45_000879"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL0622662.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            2303..3469
                     /locus_tag="PWN45_000880"
     CDS             2303..3469
                     /locus_tag="PWN45_000880"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL0622663.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(3639..4160)
                     /locus_tag="PWN45_000881"
     CDS             complement(3639..4160)
                     /locus_tag="PWN45_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL0622664.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            4569..6674
                     /gene="clpL"
                     /locus_tag="PWN45_000882"
     CDS             4569..6674
                     /gene="clpL"
                     /locus_tag="PWN45_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDL0622665.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDSALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(6743..6913)
                     /locus_tag="PWN45_000883"
     CDS             complement(6743..6913)
                     /locus_tag="PWN45_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL0622666.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(6926..8920)
                     /gene="feoB"
                     /locus_tag="PWN45_000884"
     CDS             complement(6926..8920)
                     /gene="feoB"
                     /locus_tag="PWN45_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL0622667.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(8932..9159)
                     /locus_tag="PWN45_000885"
     CDS             complement(8932..9159)
                     /locus_tag="PWN45_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL0622668.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(9375..11864)
                     /gene="farE"
                     /locus_tag="PWN45_000886"
     CDS             complement(9375..11864)
                     /gene="farE"
                     /locus_tag="PWN45_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDL0622669.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            12009..12557
                     /gene="farR"
                     /locus_tag="PWN45_000887"
     CDS             12009..12557
                     /gene="farR"
                     /locus_tag="PWN45_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDL0622670.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(12634..12801)
                     /locus_tag="PWN45_000888"
     CDS             complement(12634..12801)
                     /locus_tag="PWN45_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622671.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(12913..14457)
                     /gene="pruA"
                     /locus_tag="PWN45_000889"
     CDS             complement(12913..14457)
                     /gene="pruA"
                     /locus_tag="PWN45_000889"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDL0622672.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(14647..15246)
                     /locus_tag="PWN45_000890"
     CDS             complement(14647..15246)
                     /locus_tag="PWN45_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0622673.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            15485..15676
                     /gene="cwrA"
                     /locus_tag="PWN45_000891"
     CDS             15485..15676
                     /gene="cwrA"
                     /locus_tag="PWN45_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDL0622674.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            15916..18324
                     /locus_tag="PWN45_000892"
     CDS             15916..18324
                     /locus_tag="PWN45_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL0622675.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            18615..18821
                     /gene="copZ"
                     /locus_tag="PWN45_000893"
     CDS             18615..18821
                     /gene="copZ"
                     /locus_tag="PWN45_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL0622676.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(18911..19909)
                     /locus_tag="PWN45_000894"
     CDS             complement(18911..19909)
                     /locus_tag="PWN45_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL0622677.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(19932..21086)
                     /locus_tag="PWN45_000895"
     CDS             complement(19932..21086)
                     /locus_tag="PWN45_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0622678.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(21513..23021)
                     /locus_tag="PWN45_000896"
     CDS             complement(21513..23021)
                     /locus_tag="PWN45_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622679.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(23033..23896)
                     /locus_tag="PWN45_000897"
     CDS             complement(23033..23896)
                     /locus_tag="PWN45_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDL0622680.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(23933..25060)
                     /gene="crtQ"
                     /locus_tag="PWN45_000898"
     CDS             complement(23933..25060)
                     /gene="crtQ"
                     /locus_tag="PWN45_000898"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDL0622681.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(25066..26559)
                     /locus_tag="PWN45_000899"
     CDS             complement(25066..26559)
                     /locus_tag="PWN45_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622682.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(26552..27040)
                     /gene="crtO"
                     /locus_tag="PWN45_000900"
     CDS             complement(26552..27040)
                     /gene="crtO"
                     /locus_tag="PWN45_000900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDL0622683.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(27215..27982)
                     /locus_tag="PWN45_000901"
     CDS             complement(27215..27982)
                     /locus_tag="PWN45_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0622684.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(28343..30154)
                     /locus_tag="PWN45_000902"
     CDS             complement(28343..30154)
                     /locus_tag="PWN45_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0622685.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT         9795 a         5759 c         4671 g        10165 t
ORIGIN      
        1 taacgtcagt gcttaagacc ttgtcaatta ttgttttatc tgtttctaag agcccctgat
       61 tttctgcata catattttgt atatcgctgt taatctcatt taatttttta aatttttttc
      121 taaccttttt caaaataaaa tgctctttaa ttccatctct taaatacata taaatctcta
      181 ttataatctc cataaatgtc atatctcctc tctcaaaaaa cattatactc aatatattaa
      241 aaataagaaa ggttttttca gaatttttag tattttcaga tatttattct taagtatctt
      301 attattactt ctttttaacg ctagtggaat aattaataat aattgtattt aaatgcaatg
      361 ctgttgttct ttcatactta caagcaagtc ataagaaatg agaaattaaa ttcttatatg
      421 atgaataaat gatgactgca gaagaaattg ctaaaaaagg agtggagtga ggcattctac
      481 tgttcatatt gttaacaaat atcaaaatga taggaacttg aagaatctaa gactgttaaa
      541 atcgagacaa taagaaactc gttccaaatt tgaatcctat aatgaattcg tctcatgaag
      601 aagttattta cttttcatta gactgattta ctaatcaaaa aacttctatc acgaacgctt
      661 caatttcctg tatgcgtgtg atagaagttt ttatttttgt tcatattaat ttatctaagc
      721 gctacgatgg actgacttaa acttttcttt aaaatcgcta ttcgccattt actattgttg
      781 tctaatttct tgtaaaatat gttccgctgc ttgtgtattt gcacggggtt cttttttcaa
      841 agcttcagct actttagcaa tttcatcacc ttttgcccct acaacgatag ctaatgattt
      901 atattgtaaa ctcatatgac cttgttgaat accttctgac acaagcgcgc gacatgctgc
      961 aaagttttgc gctaaaccaa cggcagcaac tacatgacct aattcttgtg ctgactctac
     1021 atttagtagc tctaatgaag ctttagcaat tggtaatact tttgtaccac cgccaacgat
     1081 tgccaatgtc ataggcactt caattgtacc aatcaatcgt tgacgatctt gatcgtaacg
     1141 ccatgtagca ataccacgat actgtccgtc acgactcgcg tatgcatgcg cacttgcttc
     1201 tgcaccacgc gtatcatttc ctgttgctaa aacaacagca tgtatgccat tcataacacc
     1261 tttattatgt gttgctgcac gatgaatatc tacttgggcc aatacagaag cacgttccat
     1321 tcgtttggca acctcttctc cagttctctc gccccttgct aaatctttaa catcaatttc
     1381 gccttgaact ttaacaacgg acgctgttgc atgattggat aaaatactca ttaaaatgtc
     1441 gctttgcgga aattcatttt ttaaaaatgc agttatggcc tctaaaatcg tattaagcat
     1501 attagcgccc atagcatctt tcgtatcaac aaatactttt aaagatagta actgttgctc
     1561 aggaaatgta tcaatcgcta tacgttggta accaccacca cgcgctttaa tagaaggata
     1621 tgcctcatcc gcaattttat gaatttgctt ttctaaagct ttaatgtctg ctgataattt
     1681 ttcagtatcg tcaacgccat caaagacgat ttgacctatc ataatacgtt cagaagatac
     1741 cgttttaaat ccgccagtct gattcactag ctttgcacca taactagctg cagcgacaac
     1801 tgaaggctct tccaccatca taggtacaac atatgcctta tcgtccacaa tgatattcgg
     1861 taataatcca acgggtaatg caccttgcgc gatgacattt tcaattaaac tattggctac
     1921 ttcttcatcg attaatggat gattcagtaa aatgtcgaat tgttcttctg ataaccattg
     1981 cttatcaacc aattgttgta acttttcttt acgagataaa tgtcgaaaat tcttatctaa
     2041 attttgcatg gacgtactcc ttttacttca cataattttt aacatttcaa tcactactat
     2101 ttttaccaca aaataacgtc attcgtctta aaattcaatt gaataattgt cgttttgact
     2161 ttaaaataaa acaaggtaaa ttaaaacgct tacaagaaac gacaaatcat ttttaaattt
     2221 agtatatttc tttgtataaa attagcatat tctgatatga tacaagtgtt gcttttataa
     2281 atttgaaagg atgtaaaacc ttatgacaat aggtatcgat aaaataaact tttacgttcc
     2341 aaagtactat gtagacatgg ctaaattagc agaagcacgc caagtagacc caaacaaatt
     2401 tttaattgga attggtcaaa ctgaaatggc tgttagtcct gtaaaccaag acatcgtttc
     2461 aatgggcgct aacgctgcta aggacattat aacagacgaa gacaaaaaga aaattggtat
     2521 ggtaattgtg gcaactgaat cagcagttga tgctgctaaa gcagccgctg ttcaaattca
     2581 caacttatta ggtattcaac cttttgcacg ctgctttgaa atgaaagaag cttgttatgc
     2641 tgcaacacca gcaattcaat tagctaaaga ttatttagca actagaccga atgaaaaagt
     2701 attagttatt gctacagata cagcacgtta tggattgaat tcaggcggcg agccaacaca
     2761 aggtgctggc gcagttgcga tggttattgc acataatcca agcattttgg cattaaatga
     2821 agatgctgtt gcttacactg aagacgttta tgatttctgg cgtccaactg gacataaata
     2881 tccattagtt gatggtgcat tatctaaaga tgcttatatc cgctcattcc aacaaagctg
     2941 gaatgaatac gcaaaacgtc aaggtaagtc gctagctgac ttcgcatctc tatgcttcca
     3001 tgttccattt acaaaaatgg gtaaaaaggc attagagtca atcattgata acgctgatga
     3061 aacaactcaa gagcgtttac gttcaggata tgaagatgct gtagattata accgttatgt
     3121 cggtaatatt tatactggat cattatattt aagcctaata tcattacttg aaaatcgaga
     3181 tttacaagct ggtgaaacaa tcggtttatt cagttatggc tcaggttcag ttggtgaatt
     3241 ttatagtgcg acattagttg aaggctacaa agatcattta gatcaagctg cacataaagc
     3301 attattaaat aaccgtactg aagtatctgt tgatgcatat gaaacattct tcaaacgttt
     3361 tgatgacgtt gaatttgacg aagaacaaga tgctgttcat gaagatcgtc atattttcta
     3421 cttatcaaat attgaaaata acgttcgtga atatcacaga ccagagtagt cggtgtattt
     3481 aaaacacata taataaaacc taaaagcagc agtaagacca cttctaattg aaatcgtctt
     3541 actgctgttc tctatttata acacttcgta ttgaatgaat tcattatgcc tatttgacac
     3601 attattgaag ttttcctaat gcctggatcc tttatacgtt acggcttcgt gctatgtttt
     3661 ggtacataaa gctttgacat atcgatattc tccaactcta acagcttaat tttattatta
     3721 atcgttccac cgaaccctgt taagctaccc gttttaccga caacacgatg acatggcacg
     3781 ataatagata atggattact tccgactgca cctccaaccg cttgggctga catttttggc
     3841 ttgttaagca gcttgcctac ttttttggca atagcaccat acgttgttag agtcccataa
     3901 ggaacctgtc ttaattcatt ccaaacacac tgttgaaaat gactacctgt tggctttaaa
     3961 ggtattgtga tttcaggatt gtcaccttta aaatacgcgt ctaaccactg tgtcgcctct
     4021 ctaaatatcg ctaaagacgt attttcttcc ctagtaccat caccttgttg attttcaaac
     4081 aaaacagcgg tcagacttac cccatcactc aaaagttcca atcgtcctac aggcgaatca
     4141 tagtaactct tatactccat aaaaatcccc cctttttcta atcatacgcc ttaattgtta
     4201 aattagaaaa tattcatgtt cacatttttt aaaaataaat aagtcgtcaa ttacgtatat
     4261 aaacacgtaa taccagctat cactttgctg caatatacag ttacatatct tactacacgt
     4321 gctaacctct tactttgtaa accaaatctt aaattaaaat attgaaaatg caatgaatcc
     4381 ttaatatttt attaaaccta taattactta ttaaaaataa cacacaatat tcataaagtt
     4441 ttaaaaatat tctgttttat cacctactat tagtggaaaa gtacaattgc aattgtatat
     4501 agtttgcata acgcttcaaa agtaatttct tttttgttta gttcaaaaaa atttagaggt
     4561 gatgttatat gaataacggt tttttcaata gcgactttga ttcaattttt cgaagaatga
     4621 tgaaagatat gcaaggttca aatcaagtcg gaaacaaaaa gtactatatt aatggtaaag
     4681 aagtttcacc tgaagaacta gcgcaactca cacaacaagg tggcaatcac tctgctgaac
     4741 aaagtgcgca agcttttcaa caagcagcac aaagacaaca agggcaacaa ggtggcaacg
     4801 gcaattattt agaacaaatt ggtcgtaacc ttacgcaaga agcacgtgac ggtttattag
     4861 atccagtcat tggtcgtgat aaagaaattc aagaaactgc tgaagtttta agtagacgaa
     4921 ctaaaaacaa tcctatatta gttggagaag ctggtgttgg taaaactgcg attgttgaag
     4981 gtttagcaca ggcaatcgtt gaaggaaatg taccagcagc aatcaaagac aaagaaatta
     5041 tttctgtaga catttcatca ttagaagctg gaacgcaata tcgtggtgct tttgaagaaa
     5101 atattcaaaa attaatcgaa ggtgttaaat cttcacaaaa tgccgtacta ttctttgatg
     5161 aaatccatca aattatcggt tcaggtgcca caggaagtga ttcaggtagc aaagggttat
     5221 ctgatatttt gaaacctgca ttaagtcgtg gtgagatttc tattattggt gcaacaacac
     5281 aagatgaata tcgaaacaat attcttaaag attctgcatt aacgcgcaga tttaatgaag
     5341 tgcttgttaa tgaaccaagc gctaaagata ctgttgaaat tttaaaaggt attcgcgaaa
     5401 aattcgaaga acaccatcaa gtaaaattac cagatgacgt attaaaagca tgtgttgact
     5461 tatcaattca atatattcca caacgattat taccagataa agcaatcgat gtgttagata
     5521 ttacagcagc acatttatct gcgcaaagtc cagctgtcga taaagttgaa actgaaaaac
     5581 gaatttctga attagaaaat gataaacgta aagcagtaag tgctgaagaa tataaaaaag
     5641 ctgacgacat tcaaaatgaa atcaaatcat tacaagataa attagaaaat agtaatggtg
     5701 aacatactgc tgttgctaca gttcatgata tttcagatac tattcaacga ttaactggta
     5761 ttccagtttc tcaaatggat gataacgata ttgaacgttt aaaaaatatt tctaatcgtt
     5821 taagaagtaa aatcataggt caagatcaag ctgtagaaat ggtttcacgt gcaattcgcc
     5881 gtaatcgtgc tgggtttgat gacggcaacc gtccaattgg cagtttccta tttgttggcc
     5941 ctactggtgt tggtaaaaca gagcttgcta aacaattagc aattgattta tttggtaata
     6001 aagatgcact gattcgactt gatatgagtg aatatagtga cacaacagct gtttcaaaaa
     6061 tgattggtac aactgctggt tatgttggtt atgatgacaa ttcaaatacg ttaactgaaa
     6121 aagtacgccg taatccatac tcagtcattc tatttgatga aatcgaaaaa gcaaatccac
     6181 aaattttaac attgttatta caagtaatgg atgatggtaa tttgactgat ggtcaaggta
     6241 atgtcatcaa ctttaaaaat acaattatta tttgtacatc aaatgctggc tttggcaatg
     6301 gcaatgacgc tgaagaaaaa gatattatgc acgaaatgaa aaaattcttc cgccctgaat
     6361 tccttaaccg cttcaacggc atcgttgaat tcttacattt agataaagat gcattgcaag
     6421 atatcgtcaa cttattatta gacgatgtac aagttacatt agacaaaaaa ggtattacga
     6481 tggacgtttc tcaagatgcg aaagattggt taattgaaga aggctatgat gaagaattag
     6541 gtgcacgtcc attaagacgt attgttgaac agcaagtacg tgacaaaatt acagattact
     6601 atttagatca tacagacgtt aaacatgtgg atatagatgt tgaggataac gaattagtcg
     6661 taaaaggtaa ataacgacac tttaacatat cgcgcatcaa aaatgagcat caggtcgccc
     6721 ttgcctgtgc tcattttttt aattatttcc ctggaaaatg attcgctgtg tgctgttctg
     6781 ttccacaaca atcacgatta atgtcacatg taccacattt tccttgtttt gaacgcttga
     6841 aaaattttac tagtgtatat aaggcatatc cgaaaattgc taaaaaaatt aaaatgttaa
     6901 taatgactga cactttaacc actccttaaa caaataaatg tccgacttga taaaaaatga
     6961 atgttaagac atatgcagtg actagaggat aggcaactgc aagtgccgtc catttccatg
     7021 aataagtctc tttacggatt gctgctactg tagaaacaca aggaatatac aatagtataa
     7081 atatcataaa tgcatacgca gatagcggtg tgaattgatt ttgaatcaca ttaacaaggc
     7141 ctgcatcacc tgatgaatag ataatcgcca tcgaacttac gataacttct tttgctaaaa
     7201 atcctggcac taacgtagca cctgcttgcc atgttccaaa tccgagcggt tgcactaaca
     7261 taccaaagaa actaccaacc atatgtaaaa aactttgatt gatattcaca ttgataccat
     7321 gtggtcctac ataacttaat agccaaatga ctactgagcc gccaaaaata aatgtacctg
     7381 ctttacgaac aaagccctta gccttttccc aagtactacg ccacaacgtt ttaatggaag
     7441 gcacacggta tgttggcaat tccacaataa agattgcatt atcatttttt aaaatcgtct
     7501 tagtaagtac tgtactgact aaaaatgcca taataatacc taaaacatac aggcttaata
     7561 ctactaaaga ttgattctct ttgaaaaaga tacctacgaa caacgcatac actggcagtc
     7621 tagcagagca tgacatgaat ggtgcaatta atatcgttgt taaacgctct ttttcatttt
     7681 caatactgcg cgcagccata atactcggta cattacaacc aaatccgata atcattggta
     7741 taaaagactt cccgcttaaa ccgaacgatt ccataatacg atccattaaa accgcaatac
     7801 gtgccatata acccgaatct tctaataaag atataaagaa aaagagcaca acaatttgtg
     7861 gtacaaagac taatactgat cctacaccag caataatgcc atctgtaatt aaatcttgta
     7921 aaaatggtat aacaccaaga taattcataa tcgtcttcac actatctgta aatgtaccac
     7981 ctataaatgc atcgagttga tccgacaaag gtgtgccaat ccatgtaaat gtagtttgaa
     8041 agatcaacca cataattgct agaaagatag gcatccctat atatttatgt gttaatatct
     8101 tgtctattct agagctgaaa tattgcttat cttcatctgg atacgttacc acgtcttgca
     8161 ataacgtctc aatataatga ttgcgtatac gctccatctc tcgacgaaca gatacagccc
     8221 ctacttgttc agcaacttga tcacgtaaac tcgacaattt atttacaacc tctgaattaa
     8281 gttcgtttgc aatttgcatg ttatttaata agaattgaat cgcaataaac ctagcttgat
     8341 acttatcatg agatgtctct gtcattatta tttgacacat atttttaatt gtctcttcaa
     8401 tcttctcacc ataattgatt ttaaaatgcg gttgataccc ttcccctaga tgctttattt
     8461 cgccaagtaa atattttgtt cctttgcctg tacgtgccac aactggaaaa ataggtgttt
     8521 ttaacttttt catcaattta tgataatcga tttttatccc gcgctttgta gctacatcaa
     8581 tcatatttaa tccgatgtat attggttgat taagttctaa caattgtact gttaattgca
     8641 tatttctttt tagttgactc gcatcaacaa tgttaatgat tcctgaaaat gaatcgttta
     8701 ataaatagtc tgtcactaca gtttcatctt tagaaatcgg cgataaatca tatgtacctg
     8761 gtaaatcaat taattgtcct acattttctt taagtttccc tacttttttc tctaccgtta
     8821 cgccactcca gttgcctata tattcatacg aaccagttaa agcgttaaac aaagatgttt
     8881 taccaacatt aggatttcct aaaatacaat aattttccat tcgtccggct cctattcttc
     8941 taatgcaata gaacaagcat cgcaatgtct aatacttaac tgttgtccgt ttacttcaat
     9001 aatacatggc cctttaaata aacatttttg tttaatcgtt atgatagcgt catctgttaa
     9061 cccaaaggca cttagacgat acaacatatt ctcattagca atatccattc gctttatttt
     9121 ataagcctta ttcatttcac catttttaat gtttaacata ctatttcgct ctcctattag
     9181 aaataataat cattatcact taaaaatcat aacccttaaa attgtagctc gcaatacttt
     9241 atttaaataa ttttcatttt tcatgtaaaa tttgtgacat tgcaaaaatg ttttgtataa
     9301 tcacttccat gcaaaaaacc tccgcttcaa atgtcattga tagacattta aagcggaggc
     9361 atgtacatgt tattttatga tttatttcgt agattttttt ctaacaattg attgagcttt
     9421 tcaatatttt tattcaactg ttctatattc gttgcttgtt tttttgaatt tagttgatgc
     9481 ttgttttgat aatctattaa agcatcatat aagaacaatg aacttgctga ttgctgtttc
     9541 acttcttggt acaatgccat cgttttatca tctagtacta ttgaatgtgc atcatcttgc
     9601 ccatgtgcat cttgatgttt cgatacattt gttgcacctt gtggatatac tttaaaggtt
     9661 gtgtcgtatg tttccttact atctttagct tcagattcct gtgattcaac cgttttatat
     9721 ttttcaagtg catgtccttc aatatcaact cgtggaataa tgcgattcaa ccatgctggt
     9781 aaataccacg aaccttttcc aaacaatttc gttaatgcag gaattaacat cattctgact
     9841 acgaaggcat caaagagtac accaaacgct aatgccatac ccattgattt aatcatgaca
     9901 tcttcttgga atacaaacgc aaagaagaca ctaaacataa ttaatgcagc tgctacaata
     9961 acaggaccgc tttctttcaa tcctactttg atagaataat cattatcccc tgttttacta
    10021 tattcttcat gaattcgcga cataaggaag acttcataat ccatcgctaa tccaaataag
    10081 atacctatag taataaccgg taaaaatgct agcattggtc ctgtcgtttc aataccaaac
    10141 agacctttca taaaaccatc ttgcattact aatgttgtaa atcctaatgt tgccattaat
    10201 gacaagacga atcctaaaac tgcttttaat ggtattagaa ttgaacggaa gacaatcatt
    10261 aataagaaaa atgctaatac aacaatgact gaggcaaata aaggtatcgc ctcatttaac
    10321 tttttagaca tatcaatatt aatgacactt tgtcccgaaa tctccgtttt gaacccatat
    10381 ttatcttgtg catctttatg ataatctcgt aaatcatgca ctaaatcatt tgtactctct
    10441 gcattaggcc cttgcttagg tatcacgacc atcaaagcgt aatcattatc tttactcatt
    10501 tgtggtggcg taacgatatc tacatttttc ttatctttaa tatctttata tacagactgt
    10561 aaatcttgtt gtaatccttg tggatcatcc tttttatctt tcacatttat caacatcggt
    10621 atttggccat taaatccttc accaaattta tccgagataa tatcgtaagc ttttttctgt
    10681 gtagaatctg ctggtttaac accgtcatct ggaataccaa gtcgcatatg actaactggt
    10741 attgcagctg ctactaatat gattaaacct agtaatactg ccgcaagtgc atttcctgta
    10801 ataaatttag accatggcgt atcaatatct tttttgaatt tagactgtaa tttattcact
    10861 ttaatgcgtt tatggaaaat gcttattaat gcaggtaata aagttaaagc gctaagtact
    10921 gcaaaaacaa cactaattgc cgaagcaaat cccattaccg ctaagaagtc aatgcctact
    10981 aatgataaac cacatactgc aattacaact gttacaccag caaaaacaac tgcactacct
    11041 gctgttccta ttgcaagacc aatgccttta atgtaatctg tttcagtttt cataacttgt
    11101 cgatatctga ataaaataaa taatgcataa tcgataccaa ctgctagtcc aatcattacg
    11161 gctaatgtca gtgtgacatt tggtatatcg aatgcataag ttaacaaact gataatacct
    11221 acaccagagg ctagaccaat caatgcactt ataattggta atcctgcagc aatgactgaa
    11281 ccgaatgtga ttaacagtac aacaaatgca acaataatac caactagttc agaattaccg
    11341 cctacttctg tagatgtcat tcctgtacca gttaactctg tttgtacatt atgattgtca
    11401 cgtaagtctt tcaaatgact tttaacatta tctctagagc catcttttaa agatgtttga
    11461 ctaacgtcat atgtgatatc tgcaaatgca gttgttttat ctttactaat ttgcttattt
    11521 tcataaggat ctgatatttt atcaatgtgc ttgtcatctt ttttaatatc atctaacgtt
    11581 ttcttaatat ctttagtaat gttcggttgc acaataccat catctttagt cgtcttaaag
    11641 acaacacgta tttgtgcctt ttcactatct tgattaaaat gtttttcaat ctttttattc
    11701 gtatctaacg actctaatcc tgtcatttta atatcattgt caaatttcgg tgcatttgta
    11761 gcaagtggta tcaatattgc agctacaatc actatccatg caatgaccgc ggaccattta
    11821 tgttttgcga tgaatgtccc catcttatat aaaaattttg ccaaagtata ttgcctcctt
    11881 ttaaaatcaa cgttatagtt taaatataca gtgtagatta ttgttcgatt atagtatcta
    11941 tccccgacct cttaaagaat caattggaaa attttgtata ttaaactaca cacaaaggag
    12001 aaatgtagat gaaagagact gatttacgag ttataaagac aaaaaaagca ttgtcgagta
    12061 gcttgctaca attgttagaa cagcaattat tccaaacgat tactgtcaat caaatttgcg
    12121 acaacgcact cgtacaccgt acaacatttt ataaacattt ttatgataaa tatgatcttc
    12181 tagagtactt gttcaatcaa ttgactaaag actactttgc tagagatatc agtgaccgtc
    12241 ttaatcatcc attccaaacg atgagtgata cgattaataa taaagaggat ttgagagaaa
    12301 tcgcagaatt ccaagaagaa gacgctgaat ttaataaagt attaaaaaat gtctgcatta
    12361 aaattatgca taacgatatc aaaaataata gagaccgtat cgatattgac agcgacatcc
    12421 cagataatct catattttat atttatgact cgttgattga aggttttata cattggataa
    12481 aagatgaaaa aattgattgg cctggcgaag atattgataa cattttccat agattaatca
    12541 atattaagat taaatagtag atgagaaact catgagcgtt accaacattc ataataaaaa
    12601 cgatagtgta cacgttaatg aattcgtgta ctactatcgt tttttatttt tatcgtgctt
    12661 atcgctatta aaacaactga tacacaacac ataaactatg aagaaaaaaa taaatccgct
    12721 atctaaatga ctttgactca gttgtttaaa tgaccaaatt gctaatacaa ttcccattat
    12781 tattgaaata acgtatctca cattcttata cctataatcc ttttctaaaa atatggttgc
    12841 tattacttaa tttttaaagt tataaataaa aagagccaac cgcaatggat ggcccttgtt
    12901 cattatgaag cattagaaca tttctgaaac aaccttttgt tctaagaagt gtaataagta
    12961 gtctggacta cctgttttag cgtccgtacc tgacattttg aaaccaccaa atggatggta
    13021 tccaacaact gctgaagtac agcctctgtt aaggtataaa ttgcctacat caaattcgtt
    13081 taccgcttta atccaatgct cgcgattatt tgtaatcact gcaccagtta aaccgtaatc
    13141 tgtatcattt gcaacctcaa ttgcttcatc aaaatcgtta actttcacaa agccaacaac
    13201 tggaccaaaa atttcttctt gcatgattct atctttagat ttaagtcctg aaatgattgt
    13261 tggttctaca aagtaacctt ttgaatcatc agtgccgcca ccttgttcta atttaccttc
    13321 ttctttacca atctcaatat aatttttaat cttatcaaat tgttttttat taataactgg
    13381 gcccatatac gtattgtcta cagtattgcc caacgttaat tcttttgtta atttgattga
    13441 tttctctaat acttcgtcat aaacgtcttt atgcacaatt gcacgtgaac atgctgaaca
    13501 tttttgacca gaaaaaccaa atgctgacgt tacaatagct tctgctgcca tatctgtatc
    13561 aatattttca tcaactacaa tggcatcttt accacccatt tcagcgataa cacgtttcaa
    13621 gaagttttga ccttcttgaa caacggcact acgttcataa attctagtac ctgtcgcacg
    13681 tgatcctgta aatgtaacga aatgcgtatc tttatgatca actaagtaat caccaatttc
    13741 tttcggatca ccaggaacaa agttaactac gccttttggt aatcctgctt cttctaaaat
    13801 ttccattaat ttataagcga tataaggtgt atcctcagca ggtttcaata acactgtatt
    13861 acctgccaca actggtgcta aagttgtacc agccataatc gcaaacggga agttccacgg
    13921 cggaattgta acacctgtac caattgattt atagaaatat ttattgtgtt caccttcacg
    13981 atcaagtact ggcttacctt gagccaagtc catcattgaa cgtgcatagt attcaataaa
    14041 atcaatacct tcagctgcat caccaactgc ttcatcccat ggcttacctg cttcataaac
    14101 cataattgct gcaatttccg cttttcgacg acgaataatt gccgaaacac gtaacataag
    14161 ctctgcacga tcatttgctg accatgtttt ccaagattta taagcttcgt ttgctgcttt
    14221 aaacgcatct tcaacatctt gttttgttgc ctttgatgca tttgcaatca cttgtgatgt
    14281 gtctgcagga ttgattgatt taattttgtc atctttgaaa atcttctctc cattaatcac
    14341 taatggtatg tcttgaccta attctttttc cacgtctttc aatgctttct taaacatatc
    14401 cacattttct tggactgaaa aatcgtaacc aggttcattt ttaaattcta ctaccatgta
    14461 cacttacccc ctataaattt tgaaagtggt ttaacccttt gatttaatga tataacatca
    14521 tttaaactca ttttactatg attaaggtta gttttgcaat cgctttcatt tttatgtttt
    14581 atcacttatt ctcaagtatt ttgaaattga ttggttactt tttaaaattt atatgggtcg
    14641 caactgctac tttatcgttt cgtcatttaa tgtttcggat ggtaggtcat tatcaatttt
    14701 acgaacgact ttacaagggt ttccaaccgc taagctgtgt ggcggaatat ctttagtgac
    14761 aacactacca gcaccaatca cactgccttc tccaatcgtc acccctggta acacggctac
    14821 atgaccgcca aaccaagtat tactgccaat atgaatgggt ccggcttttt caaaaccttc
    14881 atttctatga tggaaattaa gtggatgtgt cgctgtgtag aatccacaat taggtcctat
    14941 aaaaacatta tcgccaattg taatctgtcc accatccata aagtaacaat tggtgtttac
    15001 atagacattt tttcctagtt taacgttcca accataatct gtatcaaaag gaatcgaaat
    15061 acttacattg tctgttgttg tttgaaataa ttgatcaatt aattcctttc ttttatttgt
    15121 agcactcggt cttgtatgat ttaattcaaa gcaaatatct ttcgctcgtg cacgttcatt
    15181 gattaagtat tgatcaaagt ttgcatcgta ccatttttct gctaacattt tttctttttc
    15241 agtcattaca cctttcaact cctaataact tatttacttg tttaaaagtt aatcaaataa
    15301 accttcgcct atgcaactaa tacgctataa cattatgaaa tcatgacctt atcaccctta
    15361 tctatacaat tctcgcatca aatactgcta aagtagtaga taaattcaat actacagacg
    15421 cattcatttt ttaatctatt aacgtacaat gtgagtaaga gaaatataaa ggagtatgat
    15481 agcgatgaga atattaatta caggcacagt tgctatctta atcattctag gtttggtcaa
    15541 aacgatacaa gattacgaaa tgacaaacga cacgagtcgt cagttgtcag acaacaaaga
    15601 tgatgataaa gtcatccatc ttaataattt taaaaattta catgcgaaag aatttaaccc
    15661 atctgatttc ttttaagtca cctaagaatt gcaaatccag aagtcattta agttttacct
    15721 ttcattcata catcctttaa tattaattac gacttctttt atatagatgc taagtagaga
    15781 gattgttgtg caatgtttgc acggcaatct ctctttttct ttttaaaatt ggtaaaagta
    15841 aaacgcaacg attgacttat atacctatag ggggtacatt agacgtgtaa caatgaatca
    15901 cagggaggca ataatgtggc taatacgaaa aaaacaacat tagatatcac tggtatgact
    15961 tgtgccgcat gttcaaatcg tatcgaaaag aaactgaata aacttgatga cgttaatgcc
    16021 caagtgaatt taactacaga gaaagcaact gttgagtata accctgatca acatgatgtc
    16081 caagaattta ttaatacgat tcaacattta ggttacggtg tcgctgtaga aactgtcgaa
    16141 ttagacatta caggtatgac ttgtgctgca tgctcaagcc gtattgaaaa agtgttaaat
    16201 aaaatggacg gcgttcaaaa tgcaacggtc aatttaacaa cagagcaagc taaagttgac
    16261 tattatcctg aagaaacaga tgctgataaa cttgtcactc gcattcaaaa attaggttat
    16321 gacgcgtcta ttaaagataa caataaagat caaacgtcac gcaaagctga agcgctacaa
    16381 cataaattga ttaagcttat catatcagca gtattatctt taccactatt aatgttaatg
    16441 tttgtacatc ttttcaatat gcatatacca gcactattta cgaatccatg gttccaattt
    16501 attttagcta cacctgtaca atttattatt ggatggcaat tttatgtagg tgcttataaa
    16561 aacttaagaa atggtggcgc caatatggat gtacttgttg ctgttggtac aagtgcagca
    16621 tatttttaca gtatttatga aatggttcgt tggctaaatg gctcaacaac gcaaccgcat
    16681 ttatactttg aaacaagcgc cgtactaatt accttaatct tattcggtaa gtatttagaa
    16741 gctagagcga agtctcaaac aaccaatgcg cttggcgaat tattaagttt acaagctaaa
    16801 gaagcacgca ttttaaaaga tggtaatgaa gtgatgattc ctctaaatga agtacatgtt
    16861 ggagatacac ttatcgttaa accaggtgaa aagatacctg ttgatggcaa aattattaaa
    16921 ggtatgactg ccatcgacga atctatgtta acaggtgaat ctatccctgt tgagaagaat
    16981 gttgatgata ctgtaattgg ttcaacgatg aacaaaaacg gtactattac tatgacagca
    17041 acaaaagttg gcggggacac tgcgttggca aatattatta aagttgtcga agaagctcaa
    17101 agttctaaag cgccgattca acgattggca gatattattt ctggttattt cgttcctatc
    17161 gttgttggta tcgcactatt aacatttatc gtgtggatta ctttagttac accaggtaca
    17221 tttgaacctg cacttgttgc gagtatttcc gttctcgtca ttgcttgtcc atgcgcattg
    17281 ggacttgcta caccaacttc tattatggta ggtactggtc gcgctgctga aaatggtatt
    17341 ttatttaaag gtggcgagtt tgttgaacgc acacatcaaa ttgataccat cgttttagat
    17401 aagacgggta ccattacaaa tggtcgtcca gtcgtgacag attatcatgg tgacaatcaa
    17461 acgctacaac tacttgctac tgctgaaaaa gattctgaac acccattggc agaagccatt
    17521 gtcaattatg caaaagaaaa gcaattaata ttaactgaga caacaacatt taaagcagta
    17581 cctggccatg gtattgaagc aacgattgat catcactata tattggttgg taaccgtaaa
    17641 ttaatggctg acaatgatat tagcttgcct aagcatattt ctgatgattt aacacattat
    17701 gaacgagatg gtaaaactgc tatgctcatt gctgttaatt attcattaac tggtatcatc
    17761 gcagtggcag atactgtcaa agatcatgcc aaagatgcta taaaacaatt gcatgatatg
    17821 ggcattgaag ttgccatgtt aactggcgat aataaaaaca ctgctcaagc cattgcaaaa
    17881 caagtaggca tagatactgt tattgcagat attttaccag aagaaaaagc tgcacaaatt
    17941 gcgaaactac agcaacaagg taagaaggtt gcgatggttg gtgacggtgt aaatgatgca
    18001 cctgcattag ttaaagctga tatcggtatc gccattggta caggtacaga agttgccatt
    18061 gaagcagctg atattactat tcttggtggc gacttgatgc ttattcctaa agccatttat
    18121 gcaagtaaag caaccattcg taatattcgt caaaatctat tttgggcatt cggctataat
    18181 attgccggta tccctatagc tgcattgggc ttacttgcgc catgggttgc tggtgctgca
    18241 atggcactaa gttcagtaag tgttgtcaca aacgcactta gattgaaaaa gatgcgatta
    18301 gaaccacgcc gtaaagatgc ctagattcct taataatgaa ggattcgttg gtgattctga
    18361 gataggctag tgattggctc tataatgtcg cggtttacag ttggatcttc gctccaactg
    18421 cataagagcc actaattatt ataaataata agtacactac ggtttacagt tggatcttcg
    18481 ctccaactgc ataagagcca ctaattatta taaataataa gtacactacg gtttacagtt
    18541 ggatcttcgc tccaactgca taagagccac taattattat aaataataag tggctctaaa
    18601 aggaggtgaa tgctatgtca caagaaattt taaatgttga aggtatgagc tgtggtcact
    18661 gcaaaagtgc tgttgaatct gcattaaata atattgacgg tgtcacttca gctgacgtta
    18721 accttgaaaa tggtcaagta agtgttcaat atgatgacag taaagttgct gtatctcaaa
    18781 tgaaagacgc aattgaagat caaggttacg atgtcgttta attaggcaat attcaacgtc
    18841 atcaacacca aattaaaaaa tcgaactgat gagaatccca acaatccaaa ttatctcatc
    18901 agttcgattt ttaatttact cgtaacctag tatctccagt ctgcaataca tctaatgttg
    18961 catctaatgc atcgacaatt agatttttaa ctgcagcttc agtataaaac gcaatatgtg
    19021 gtgttaatat gacatcttcc ctgtcaatca acgattctaa caatggatcg ttcagtgttt
    19081 tgcccctttg atcacttggg aaaagtttgc gttcaaattc atacgtatca agtgctgcac
    19141 ctttaatcac accattgtct aatgcgtcta ataacgcctt agtatctact aaagaacctc
    19201 tcgcacaatt gacaaatact gcgccctttt taaaatgttt aaataattca gcattaaata
    19261 gataatgatt atatttcgtt gcaggtacat gtaatgtcac gatatcagca ccttcaaccg
    19321 cttcctcaat cgtatctttg taatcgacat acgttgcaat tttagcatta ggaaacgggt
    19381 cgtatgcgac cacatcactt tgataaccat tggcaaatat atcggctact acacggccaa
    19441 ttcgacctgt accaataaca gctactttta aatctttaat ggatttcgat aaaatagtag
    19501 gttcccatct aaaatcatgc tcccgcactt tcgtttgaat ttgattaaaa tgacgaacca
    19561 cattaatagc ctggttcaca gcaaactccg caattgaatt cggagagtat gacggcacat
    19621 ttgacacaat aaagttatac ttgtttgcta actccaaatc atatgtatca aatccagcac
    19681 tacgttgtgc gatttgttta atacctagtt catttaatcg tttataaaca tgctctgata
    19741 atggtatttg ttgtgatagc gataagccat cataaccagc gacaccttca acattgtcat
    19801 cagttaatgc ttctttagta atatctacct caacatgatg tttctctgcc cacgccttga
    19861 tataaggcat atcttcatca cgtacactca tgattttaat ttttgtcatt ttaacatcac
    19921 ccttaacttt attattcata taaatatgct agttctgtta atcttattgc agcttcgtct
    19981 aatttctggt catctaacgc caatgaaatt ctcacataac gattaccatt ctctccaaat
    20041 ggtttccctg gagcaacaag tattgacttc tcttgcacta aaaattgctc aaattgctcg
    20101 ctgtcataac caggcggtgt ttccaaccat acatatatgc cacctttagc atgaacaaat
    20161 ggcaaatcag cttttgcaag catggcttcg aatcggtcac gacgtgtttt aaatacattg
    20221 ctttgttctt ctaaaaaatc atcataatga ttcaaagcat atattgcggc atcttgtaat
    20281 gcaccaaaca tcccagcatt tgtgtgcgtt tggtactttt tcaaagcttg aatcatatct
    20341 ttattaccaa ctgcaaaacc gactctaaaa cctgacatgt tataaccttt agacaaggaa
    20401 taaatttcaa tcgcaacatc tttcccattt tccgaagcaa gtatactagg atttttagcg
    20461 tcgaaaccga aagcaccata agcaaaatca tgcacgattt tagtgtctgt acctttaaat
    20521 ttagctatcg cttcatcaaa aacttctttc gtagctgtcg atccagttgg attatttgga
    20581 tacgttaaat aaatgagttt tgttttatct attatttgtg aatcaacttt ggaccaatct
    20641 ggcaaataat gtggcggttc taaattaagc gggactggct tgccatcagc taaaagtaca
    20701 cctgctaaat aatccgtgta gcctggatca ggtagtaata catagtctcc tggattgata
    20761 acacatgttg gtactgccac taatccattt tttgtaccat ataaaatgca tacttcatct
    20821 tctttatcta acgtcacatt atattgtctt tgataaaaat ctacaatagc ttgcttgaac
    20881 gcttctttac catgaaaagc accatatttt tgattttcag gaatagttag tgctttttga
    20941 aaatgatcaa taataccttg tggcgtgggc ccatcaggga ttccaactgc catattaatt
    21001 aatggcaatg gtccatgttc gattttacgt cccatcgttt tcccgaaata actatcaggg
    21061 atatttgcta atttgttaga gatcatcaaa ttcctcctct atcattaaac atagcctggg
    21121 cgactatcat aatcctaaca acttgtatca ctctcattta gatggttaca atgacatcgc
    21181 cattcaccgt tatgttcaac agaacttatg acacacgttg tattgaatga atttattttc
    21241 attttaggta ggtataatat tattgtcaat attaggaatt ttcagattaa tatgcactca
    21301 atcgttatga tttaactgtc atgcatatcc gcatgcgcaa ccagttagat atgcttatat
    21361 aaagtataac gcccatcaag gtacgtattc aaacgtgaac cttaacaggc gtcattcatt
    21421 gttaaataaa acttcttaag cacatactta tttcactatg ccttttacgt tccccttata
    21481 cttttctcac atctttctct tagactactc ccttatacgc cccgctcaat atctttaatc
    21541 atttcatcta cagttatttt cgcactcgtt aagacaatag gaacgcctgc acctggatgc
    21601 gtacttgcac ctgcaaaata taaatcttta taatctcgcg atacattttg tggacgataa
    21661 taattacttt gcgctaaagt tggcattaaa ccgaatgccg aaccaaattt cgcatgatac
    21721 gtttgctcaa aatcatttgg cgtaaagatt gtttctgaaa caatatgcga ttttatatct
    21781 tcaaatactt caatcgttgc taatttacga taaataattt cctttatttg ttgcgtcaaa
    21841 gcttcatctg accaatcgat tccgctacct gttttaagtt ccggcgtcgg cattagcaca
    21901 taaataccag ttttgccttc tggcgcaagt gatttatcag cgaccgctgg tacatacaca
    21961 taaatagaag gatcatatga taaacgtccc tcaaatattt cttcaatatt gcctctaaag
    22021 tcatctgaaa aaataacatt atgaagtctc acttgatctg tcacatcaat atctataccg
    22081 atatacatta aaaatgctga acaagagtaa tctaagtctg caattttatg tggtggatac
    22141 tttttaatag gtgcaaaatc tggcattaat gattccgcaa cactagggaa atcagccgta
    22201 cataaaattt tatcaaattt tcttatgtca ccattcactt ttatcgcatc ggcccgtttg
    22261 aatttaggat caataataat ttgctcaatt tcagcattta gttcaatatt aacgcctaag
    22321 tctttattta attgcgctag cccttgagcc atgccataca taccgccttt aataaaatgc
    22381 acaccaaaca tcatttcaat cataggaata attgaatata gtgacgggcc tcgttttgga
    22441 tcaattccta tgtataacgt ttgaaacgct aaaagctttt gtatcttttc gttatcaata
    22501 taatgttcaa ttagctgatc tgcatgattt aacgttttta acttagcacc ttgcacaagt
    22561 gacgtcatat tataaaagtc actcggtttg cgatacgttc tttctaagaa atagcgacgt
    22621 gcaatttcat attttttata aacatccgtt aaaaaggaca taaaaccatg cgttgaacca
    22681 ggttctatac tttctagcat ttgctgtaat tcagctaaat ctgtaggcac cgttatacga
    22741 tcatcgtggt caaaatacac atcgtaaata taacgtaatt gtctcaattc aatataatct
    22801 tcataatttt taccacacgc tgtaaaaaca tctttataaa catctggcat catgacaatt
    22861 gtgggaccca tatcaaatgt aaagccgtct ttctttaatt gattcatacg cccgcctaca
    22921 ttattatttt tttcaaatat cgtcacttca tgaccttgag aagcaatacg ggctgccgct
    22981 gctaatcctg tgacacctgc accaattact gcaatcttca ttattcaacc acctatattc
    23041 tatgatattt actatttatt tcatgaaaca actttgcctt tttcctctta tccacaaaaa
    23101 cacgttcatg taatgtatag ttagcctgtc tcacttcgtc cagtatttca atatatatac
    23161 gtgctgctaa ttctatgatt ggttgtgctt caatactaaa tactttgatt tgatccataa
    23221 catcttgaaa atctttttct gcgatagctg cataatattc ccataagtca atataatgat
    23281 tattaacacc attttggtac acttcagcaa tatcaacttc atattgcttt aatcgttgct
    23341 tactaaaata tatccgttca ttgtcaaaat cttcaccgac atctcttaat atattaatca
    23401 attgcaacga ttcaccaagt cttcttgcga catcgtatgt ctgatgtgtt tcatgatcac
    23461 ttaaaatcgg cgtcaatact tcacctactg taccagcaac accataacaa tatccgaata
    23521 attcagcgtc cgtttcaaac attgtaaaat gttgatcttt atatacagta tcaatgagat
    23581 tataaaaaga ttgaaaggcg atatttttat gttgtgcaac atgctgaagc gccatcatga
    23641 ttctacgatc actttgaaag tgatgatgtt catatgggta tttttcaata gattgtatat
    23701 cttcttttat ttgatttaaa aattgaatat cgccataaac atctatactg tcatcaattt
    23761 tacgacacac agcataaatt gcccaaaccg cttttctttg atcttctggt aacaagtcaa
    23821 aagcgtaaga aaagcttttt gaatgtttct tcatgatttt atgacaatat ttaaaattca
    23881 tatccatcat tgtcatacta gtcctcctat attgaaatgc cattaccttg atttattgtt
    23941 ctttagatat agaatattga cgacctttcc attcaactac accataacga tgcgtttgtt
    24001 tccaagattg gatgaaaatt ttagtaaaaa acataaataa caatggatga cataccatta
    24061 ataaattaga aaattggccg acccttcgat gcagataaat aaattgtgta gtatagctca
    24121 agtaaagtgc taccattttt cttacagaca tttggcgata ttttaaacta agacatagcc
    24181 ctaaaatact cgctatagaa ccaaacaacc acaacacaat tgctgtcatg atcttaggct
    24241 ttgtgccacc tgccccagtt gacaaatgct ttgtccatcc ttcttgtaat gactgaaatc
    24301 cttcttgata catgcgaaat gcaacaaatg gaaacccttc ataaactgtt acgggcaatg
    24361 attgtgaagt atatgcactt cctaaagcaa atccttcaat aatatgacgg tttgcacttt
    24421 tatgacctcc agttgcataa taatcttctt tatttgttaa tgtcaccggt ccaaatgcat
    24481 gctggttagt ccgaccgtcg tctaaggtag aaaatacatt cataccaacg actgtcatta
    24541 aattaaatat cgctgaaaac ccttcgtaga aacgctttgt tatatgataa ggctgtacgc
    24601 ttaacaatcc tttttcacct tgtaattgat actgattaat caacgtttca acagcatctt
    24661 tccttaagaa agttacatca gcatctacaa aggcaatgcg attcgtacat gcatgcgtca
    24721 caccttgata acaagcatgt gatttcccat accatttgcc gtcagtatca tcaacaacat
    24781 ctactacagt cgcaccatat gaacgtgcta cacgtgctgt ttcatctgtc gatccgtcat
    24841 tcataacaat gacatctact ggaacttgct gttgtattat cgaatgtagt aaatgaccta
    24901 ttcttttttc ttcgtttcta gccggaataa taattgttaa tgctttatga ttgaagttca
    24961 gcgttttcgc ctttagctga tgtctacgat taaatatcaa tgcaccacac gccatagaca
    25021 tggtcactat tactgttaat attcgtgata accatttcat agatatcact tcctattctt
    25081 cgcttctcgc gcgtttattt tgtctgcgac ttgttgccca cttaatgtaa ccattggcat
    25141 gccaccacca ggatttactg atccacctac aaagtacaag ttttcaaaat actgactttc
    25201 tttaggaaat ttaaatcctt tgtttttctt tttatctgct acaacaccat atattgcacc
    25261 acgattagaa cgataatttt tttcaatatc ctccggtgtc caaacatctt cataaataat
    25321 gtgttttctt aaatcagtaa gtcccatttt ttctaattta tccaaaattt tatccctaaa
    25381 cttcgcataa tcttcagtgg taaaaggctg atcttgaata tatggaatat gtggtaagac
    25441 tttgatattt tcataaccta ctggcgcttg tgtgtgatca gttttattcg tatttactag
    25501 ataaatggtc ggatcatctg gcaatacctt ttcatgaaaa acttgttgat aattgagata
    25561 agcattttcc gtaaaaaaga aattatgatg tgctaattgc gggtattggc aagcaacacc
    25621 taaatgcatc acatatcctg agcttgccgg ctcaaattcc ctctctaatt tgtttaatcg
    25681 ttgagtatca aggtgaatta aatatttata agtaggtatg acttccatat ttgaaataat
    25741 ataatctgcc tttacaaact cacctgtatc taatctgaca cccgtcacac gtctttgata
    25801 tgttttaata ttgtccacac gtgcacctgt atgaattgtg acaccttctt cacgcgctag
    25861 cttttccaag gcattggcta aatgatggat tccaccttct acataccaaa ggccttgctc
    25921 ttgttgcata tggaataaca tagataatac agctggcgca tcgtatgatg aagaacctac
    25981 atatttgata aaatagccta acatttgtcg caagtatgga ttcgagatac gcttgtctat
    26041 ggcctgttgc atagtatgta cataatcata attaataaga gcatttaatg gcccatgaaa
    26101 tttaataatt tgagatagtg tatctaaacc atagttgaaa tacccttttt cagtaatacg
    26161 atcgattcgt cttgtataat tcaaataatt ttgcagttcc tctatatcct gtttcgacaa
    26221 tatcgcatta tgctgacctg tttctttaat accttcatac aaatcgatag tcgttccatc
    26281 tggaaaaaag ctacgccatt gatgtggcaa tcgcttgatt gtaacgtagt ctgacatttg
    26341 cttcttgcta tattcgaata atttttcaaa aatataaggc atcgttaaaa tagatggacc
    26401 taaatcaaag ccaaagccat ctgattcatg acgattcact ttgcctccta tatgattatt
    26461 ttgttcatat aatgagaccg aatagccact ttgtgccatt cgaattgctg cagaaatccc
    26521 acctaagcca ccaccaataa cgatgatatg tttagtcatg acgttcacct cttcgtttta
    26581 atatgcgtag taactgcgtt aatctcggtc gattatagcg ttgcacaata atgattggaa
    26641 cattagctat cattgcatag aaaatattta tatactttac taaacgaggg cctttattga
    26701 atatgatgac tggaagtatc gatatccaat gaatcaactc tgccctcttt gtctctataa
    26761 tcattttttc aatttcatca gtattgattg tcattaaatg acgctgatca tatatatttt
    26821 gattaagctg atgaccatct aaaatcttat gtttccattt tcttacgtaa aaccatttgt
    26881 tccaaaattt tccatgcttc tcaaagttaa atgatttaaa tattatgtac ttctgacgaa
    26941 aatacttatc aggaattctt gtacctaaat ttgctatatt tagttgaaca attaaccaat
    27001 acatactaca aaaaaatgct gttttaatat attttttcat ggttttcatc taaattgaat
    27061 cactctcaat catactgaca tagtataaat tatcacttta ttacttagtt ccctaaagat
    27121 cagtgaataa accgctctca tttatattta tataaaacaa aaacgccaat actctcatgt
    27181 tagagaatat tgacgtcatt tgatttacat ttgattaatg aatgaaatta tatgaacctg
    27241 cttggtttgc tgaaattgta cgagacgtaa ccacaccagc accatgtcca tagttcattt
    27301 ctgaaacacg aacagaaccg ttgctgttaa cgccttcaac gtaagcaaca tgaccgtaat
    27361 agccttgtgt tgtttgcatg atagcaccaa cttttggtgt attgttcact gtatagccag
    27421 atgatgcagc tgcgttagcc caattacttg cgttacccca tgttgaacca attttcccac
    27481 caacacgatc aaatacataa taagtacatt gtcctgaagt atataagtta cttcctgatg
    27541 cataaccatt agaaattgaa cgaccatttg aagatggcgc tgcagttgta gtcacatgaa
    27601 cattattact tgttgtgcta taacttgcac ctgaaccacc agtataatag ttatttgttg
    27661 ctgtttgact attgtttgta tagttattgt attgatatga atgattatag tagttattgt
    27721 aagatgcatt gttataacta taagtattgt agttgttgta gtagtatgtg ttgttttgcg
    27781 ttaattgact tggattccaa tttcctgtcc aagtgtaatg ataattacct tgtgcatcaa
    27841 ttgtatacgt atagctgtat gattgagcgt cattagaatt atatccattg ttattttgtt
    27901 ctgcggcttg tgcatcatgt cctgcaaatg cgatagttgc aagtcctgct gtagcgattg
    27961 tagctgtaac gatttttttc ataattaaat atcctcccaa taatcaaaac actctataca
    28021 tatagtcatt agcgactcaa ttattttaaa ttttatattt tttatttttt tagtatgcaa
    28081 acaattataa cttttatagt tttgcgacaa aacaaactat aacagagatt aggtgtcttg
    28141 cgggctttgt tatcataatg taatctgttc ctaataaagc ttcattgaat gtaatatacc
    28201 aattagaatg tttacaacac tttttctgca ccgttactat gggatttgaa aataaaaaag
    28261 cactaaactt ttaatgaaat acatcatttt tgataataaa tatcagaagt aatgtaatat
    28321 ttcaaaagtt tagtgcatca aattatttct tatttgtagc atgtgtttcc atcgttttta
    28381 caatcaaatc agtcagcgct ttactacctg catattctaa gtgaataccg tcatatgcaa
    28441 agtattccgg atgacctgca gaagctttat accaatcgac tagatgtaca ttagagcgct
    28501 tttcagctgc ctcataaatt aatttattta ttctaccttc ataatctcta ggtactctaa
    28561 tagaaactaa atatatgtct gcttttccaa aactatccaa tagttcattt aattgatctt
    28621 tcgtaaatgc cccatttgta ccaagctcta ctacaacttt ttgacctttt ttagcatagt
    28681 ctttatattg cgatttcaca attggtgtag catcaacgag ttgccgtcca actttaccat
    28741 caatttgtgc atttggtatt ttcttagtaa agacattacc aatatccacc atgaccgagt
    28801 caccaattaa tagtggtgat gactttttaa tatcctcttt gctattcgct gtctgcttat
    28861 cctcttgctt atctttttta acgacttttt tcttcgttgt tttaaattcc gtcttcttct
    28921 ctttcttctc ggcttcatgt tgtttgccaa gtgcatcaaa ctgtccactg agcacaacga
    28981 tagacggaac caataatagg ataactaaca ctgttctagc aaattgcccc ttctttttag
    29041 gtaaaaatgc aaaagcttta aatccttttt tacgtatagg tgtttcaata aagcgatacg
    29101 aaatttcagc cattaacgct gttaacaaaa tttctataat ataaacgtat accggtattt
    29161 gtccttgtac gtaataactg ttcacaaaaa caatgatagg ataatgccat aagtataagc
    29221 tatatgatcg tttacctata attagtaaag gtttcatact taaaaattta gcaaataaac
    29281 tagaaggatg taccgcaatt gcaataatga ataaagttgc aaatgatata atgtaaaatc
    29341 ctccattata gatccattga tcttggtctc caactataaa gaacaaagtc attagaaccg
    29401 caaaaccaga tatccctata atatctaatg atacgacaat ctttttagaa atatcttttt
    29461 tcaaagcaaa cggaggccaa ataaatgcta atatacaacc aagcaataaa gtttgcagtc
    29521 gtgtatctgt cccaaaatac acacgtgaat tatctccagt gatgaaatga atcactatca
    29581 taagtcctaa agaaatcaac gatacaataa atagcgtttg aataatattt ctcggtttaa
    29641 atctatgtaa taagaacgtg ataaccaatg ggaaaagcaa gtaaaattgt tcttcaatgg
    29701 ctaaagacca taaatgtttg agtggttcaa tagcaaattg gttaaaataa tctacattct
    29761 gtgaaatgta ccaccagttt gaaacataga atatagctgc aatagcatct cgtttcattt
    29821 gtataatcaa ttccggttta aatatcagtg tgaacgtaag cacgacacag attaaaaata
    29881 acactgccgg aatgagtcgt ttcaatcgtc gcttccaaaa ctctagcaaa tcgatttttt
    29941 gcgtccgata atactcactt atcaacaaac ttgttattaa ataacctgaa ataacgaaga
    30001 atgtatctac tcctaaaaag cccccactta accattgtgc attcaagtga taaataatga
    30061 ttcctataac tgcgaatgcc ctcaatccat ctaatccagg taagtatcgc ggggaataca
    30121 ttttttctaa acgtttaaag tcttttgtat ccatgttaat aaacgcccca tttatttttc
    30181 tctattttgt agcatatcac aatatttttg aaaataaaat attgcactga ttttcattaa
    30241 ttgatttaac ccttaattaa gatagtttta aattttttat taagtagaaa acaattatta
    30301 cagttgattt cattactgca aaccacatat aaatttgtcg attttactac ataacataga
    30361 ttatcataga ttcttgaatt tttagcaaaa
//
LOCUS       DANJFZ010000024        28936 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.024, whole genome shotgun sequence.
ACCESSION   DANJFZ010000024 DANJFZ010000000
VERSION     DANJFZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28936
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.024"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..1448)
                     /gene="dnaG"
                     /locus_tag="PWN45_000903"
     CDS             complement(<1..1448)
                     /gene="dnaG"
                     /locus_tag="PWN45_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0622686.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFL"
     gene            complement(1509..2327)
                     /locus_tag="PWN45_000904"
     CDS             complement(1509..2327)
                     /locus_tag="PWN45_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL0622687.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(2338..2961)
                     /locus_tag="PWN45_000905"
     CDS             complement(2338..2961)
                     /locus_tag="PWN45_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0622688.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            3296..4687
                     /locus_tag="PWN45_000906"
     CDS             3296..4687
                     /locus_tag="PWN45_000906"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDL0622689.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(4837..5583)
                     /gene="recO"
                     /locus_tag="PWN45_000907"
     CDS             complement(4837..5583)
                     /gene="recO"
                     /locus_tag="PWN45_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL0622690.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(5611..6510)
                     /gene="era"
                     /locus_tag="PWN45_000908"
     CDS             complement(5611..6510)
                     /gene="era"
                     /locus_tag="PWN45_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL0622691.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(6511..6915)
                     /gene="cdd"
                     /locus_tag="PWN45_000909"
     CDS             complement(6511..6915)
                     /gene="cdd"
                     /locus_tag="PWN45_000909"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL0622692.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(6926..7270)
                     /locus_tag="PWN45_000910"
     CDS             complement(6926..7270)
                     /locus_tag="PWN45_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0622693.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(7273..7740)
                     /gene="ybeY"
                     /locus_tag="PWN45_000911"
     CDS             complement(7273..7740)
                     /gene="ybeY"
                     /locus_tag="PWN45_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL0622694.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(7741..8688)
                     /locus_tag="PWN45_000912"
     CDS             complement(7741..8688)
                     /locus_tag="PWN45_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL0622695.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(8992..9690)
                     /locus_tag="PWN45_000913"
     CDS             complement(8992..9690)
                     /locus_tag="PWN45_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDL0622696.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(9707..10696)
                     /gene="floA"
                     /locus_tag="PWN45_000914"
     CDS             complement(9707..10696)
                     /gene="floA"
                     /locus_tag="PWN45_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDL0622697.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(10714..11397)
                     /locus_tag="PWN45_000915"
     CDS             complement(10714..11397)
                     /locus_tag="PWN45_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDL0622698.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(11641..11817)
                     /locus_tag="PWN45_000916"
     CDS             complement(11641..11817)
                     /locus_tag="PWN45_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL0622699.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(12110..13456)
                     /gene="mtaB"
                     /locus_tag="PWN45_000917"
     CDS             complement(12110..13456)
                     /gene="mtaB"
                     /locus_tag="PWN45_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDL0622700.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(13463..14215)
                     /locus_tag="PWN45_000918"
     CDS             complement(13463..14215)
                     /locus_tag="PWN45_000918"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL0622701.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(14217..15155)
                     /gene="prmA"
                     /locus_tag="PWN45_000919"
     CDS             complement(14217..15155)
                     /gene="prmA"
                     /locus_tag="PWN45_000919"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL0622702.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(15159..16298)
                     /gene="dnaJ"
                     /locus_tag="PWN45_000920"
     CDS             complement(15159..16298)
                     /gene="dnaJ"
                     /locus_tag="PWN45_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL0622703.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(16434..18266)
                     /gene="dnaK"
                     /locus_tag="PWN45_000921"
     CDS             complement(16434..18266)
                     /gene="dnaK"
                     /locus_tag="PWN45_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL0622704.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(18335..18961)
                     /gene="grpE"
                     /locus_tag="PWN45_000922"
     CDS             complement(18335..18961)
                     /gene="grpE"
                     /locus_tag="PWN45_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL0622705.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(18993..19970)
                     /gene="hrcA"
                     /locus_tag="PWN45_000923"
     CDS             complement(18993..19970)
                     /gene="hrcA"
                     /locus_tag="PWN45_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDL0622706.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            complement(20070..21194)
                     /locus_tag="PWN45_000924"
     CDS             complement(20070..21194)
                     /locus_tag="PWN45_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL0622707.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            complement(21757..23580)
                     /gene="lepA"
                     /locus_tag="PWN45_000925"
     CDS             complement(21757..23580)
                     /gene="lepA"
                     /locus_tag="PWN45_000925"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL0622708.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFT
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            23926..24177
                     /locus_tag="PWN45_000926"
     CDS             23926..24177
                     /locus_tag="PWN45_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL0622709.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            complement(24222..25196)
                     /gene="holA"
                     /locus_tag="PWN45_000927"
     CDS             complement(24222..25196)
                     /gene="holA"
                     /locus_tag="PWN45_000927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL0622710.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(25253..27454)
                     /locus_tag="PWN45_000928"
     CDS             complement(25253..27454)
                     /locus_tag="PWN45_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL0622711.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            complement(27459..27920)
                     /locus_tag="PWN45_000929"
     CDS             complement(27459..27920)
                     /locus_tag="PWN45_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL0622712.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            complement(28012..28698)
                     /locus_tag="PWN45_000930"
     CDS             complement(28012..28698)
                     /locus_tag="PWN45_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL0622713.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            complement(28738..>28936)
                     /locus_tag="PWN45_000931"
     CDS             complement(28738..>28936)
                     /locus_tag="PWN45_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0622714.1"
                     /translation="MTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETF
                     TDFNIDEHNEDAERLFFIAKK"
BASE COUNT         8982 a         5378 c         3943 g        10633 t
ORIGIN      
        1 tttaaaaatg tatctttatc tctcattaaa tgttttaaaa atgctcgctc cgctttttct
       61 tgacggctca aattgtcaaa ttgtgccata ccaattggct caggttcaat gtaaccgcca
      121 tactcatctt ctggataata attggctggt gcttgattga attgtatttc gttagctaat
      181 tgctcaggac taacattgaa aaatggtgca acatcattta aagccttttg ttgcaaaatc
      241 gatgatttca taagcgaaat atcatgactt agttctttca aataacgttc atatgaaagg
      301 tcattatgtg caatttcatc ttttaatata ctcactttat aatgtgcaaa tgactttttg
      361 tcatttttta caaaagcagt aaatgcatcg ttgccatact taccaatgta ttcatccgga
      421 tccatgcctg atggcaattg tataacaaat acatttagcc cttgctgtaa caaattttga
      481 cctgttttaa gtgttgcttc actacccgca aaatccccat caaacattaa tgttatattt
      541 gatgttaact ttcgtataaa agtaatatgt tcatctgaca actgtgtacc cattgttgca
      601 acaacgtttt tcaagccagc agtatcagat tttataacat ccataaaacc ttctagtaat
      661 acgatttcat ctaattttct aattgattta cgcgctttat ctaagttgta taacaacttt
      721 cttttttgaa agataggtgt ttcaggacta tttaagtatt ttggttcttg accggtatat
      781 gttcgacctg aatatccaac aattcttcct tgcgcatttt tcaaaggaaa cataatacga
      841 tttcgaaatc tatcgtaata actgaaattt tcttcgttac gtgataataa tccggcttca
      901 tatgctaatt caatatcgta accctttttt tgaagaaaat catgacaaaa atgtgagcta
      961 tcgggtgcaa agccaatgcc tcgctcttta ataagcgcat ctgtaaaacc acgttcttgt
     1021 aagtacgtta atgcttgttc gccttcgact gtctttgtta aagcgtaata ataaaattct
     1081 tgtattaact catgcatttc aatcatttgt aaatcatcag aagcaatttg aacatttgag
     1141 ttagattgtg ttgcctcaat atctacagca acattaactc tatcacctaa ttctttaacc
     1201 gcttcaacaa atgatatgtc tttaatttct tgagtaaatt gaaaaacatt gccacctttt
     1261 ttacaaccaa aacaatgaca aatttgttta tcttcagaaa ctgtaaatga aggtgtcttt
     1321 tcatcatgaa aaggacacaa acctatataa ttgcgtcctc tcttttctaa ttttacatat
     1381 tcacttacca agtctaaaat gtcggtttta tcttttattt cattaatgat cgattgatct
     1441 attcgcaaat taaatcacct attaagcatt agttatacta ttatacctaa ttttcgacaa
     1501 aaatgaaatc atttcgattt attttgttca atataatgga ttatatcgtt tgctgtttct
     1561 tcgattgctt tttgagaaac atcaatgaca ggacatccaa tttcacttac gatttcttca
     1621 aagtaattca attcttcttg aattcgtgct tctgttgcat atcgagctgt gtcacctagt
     1681 cctaattgtt ttagtcgctc ttttctaatg cgatttaatt tttcttcact tattttaagt
     1741 gcgatacatt tctttggatt aatatcatat aagccatctg gcggtgtcac ttctggtaca
     1801 atcggtacat tcataacttt gtaactctta tgcgctaaat actgagataa tggtgtcttt
     1861 gaagttctcg aaataccaag taaaacaata tcagctttag gtaatccttt aggatcttta
     1921 ccatcatcat atttaactgc aaactctatc gcatcaattt tcttgaaata tgcatcatct
     1981 aatctatgaa cgatacctgg ctcattataa ggtttttctt caaccgaagc agataataaa
     2041 tccattaatg gccccatgat atcgacagac ttcaattgga attctgctac tttctcactc
     2101 atatattgct tcatttcagg tttaataagt gtataaacaa tgatagcatt tgtatctttt
     2161 gcaacttgaa tcacttcatc aacatcttca aaagattcaa tatatggata tcttaataat
     2221 tcatttttac attgcttagg attgaattgt gaaatacctg ccctagcaac taactctgcc
     2281 gtttcaccta tagaatctga agctacgata attttaattt tttccattac ttctcaccta
     2341 ttctttatat aatgctacta ataacttagc tattgttgtt ttggaaattc ttccaattac
     2401 ttcatacttt tgattatctt tttttcttac aattggaatc gaatcaattt ctttttcaat
     2461 cattctatct gctgcgtata tgactaattc gctttcctct aaataagtga cattaggcat
     2521 acgtgtcata tttacactga taggtacttt atgaatatct gctccaatca ttgaagctct
     2581 taataaatct tttcttgaac acacaccaac aaaatcgtta tcttcattaa taataaataa
     2641 tgtacttaca tcttctaaaa aaattgtaca aatagcgtca taaactgttg tattctctct
     2701 tagcacaaca ggttgagaca tatagtcctt aacctcaaat tgtcgaagtt tttcattaaa
     2761 aaatttgcct tttgatttac ctgaataata atatccaact cggggacgcg cttctaaaaa
     2821 acctgacatt gttaatatcg ctaaatctgg tctaagcgtt gctcttgtta aattcaactt
     2881 atctgctatt tgttcaccag taatgggtcc tttagtttta acaatttcga tgattcgttc
     2941 ttgtctttga ctgagttcta taggtcttca cccctttttt attcacctat tattataccg
     3001 atatttcttt catgtcacaa acacattaat tttacttgcc tttaaataat ctatcaatta
     3061 gaataaacgt acagcgagtt aaggatagtg taagcttaac aataagattg gcgcaacgaa
     3121 tcattttaaa ataaaagcga gtgactacac taatttgggt ggaaccgcgg gttaaatacg
     3181 taacttattt tgtattatat acaatttaaa atattaactc gtcccatgga agtaaagcat
     3241 atttactatg ctgttatttt ccatggggcg ttttcatgta tgaggagagg taattatggc
     3301 aaaagatatg gatacaattg tttcattagc aaaacacaga ggttttgtgt tccctggtag
     3361 tgatatttac ggtggtttat caaacacatg ggattatggt cctttaggtg ttgaattaaa
     3421 gaataatgtt aaaaaagctt ggtggcaaaa attcattaca caatcaccgt ttaacgttgg
     3481 tatcgatgct gcaatcttaa tgaatccaaa agtatgggaa gcttcaggac acttaaacaa
     3541 cttcaacgac ccaatgattg ataataaaga tagtaaaatt cgatatcgcg ctgataaatt
     3601 aattgaagat tatatgcaag atgttaaagg tgatgaaaac ttcattgccg atggtttaag
     3661 ttttgaacaa atgaaaaaaa ttattgacga tgaaggtatt gtttgtcctg taagtaaaac
     3721 tgctaactgg actgaaattc gccaattcaa tttaatgttt aaaacattcc aaggtgtaac
     3781 tgaagattct acaaatgaaa ttttcttacg tcctgaaaca gcacaaggta tttttgtaaa
     3841 ctataaaaac gtgcaacgtt caatgcgtaa aaaattacca tttggtatcg gtcaaattgg
     3901 taaatcattc cgtaatgaaa tcactccagg taacttcatt ttcagaacaa gagaatttga
     3961 acaaatggaa cttgaattct tctgtaaacc tggagaagaa atcgaatggc aaaattattg
     4021 gaaaactttt gcaagtgact ggttaacaag cttaaatatg agcagtgaaa atatgcgttt
     4081 acgtgatcat gatgaagatg aattatctca ttactcaaat gcaacaactg atattgaata
     4141 taaattccca tttggttggg gtgagttatg gggtatcgca agtcgtacag acttcgactt
     4201 acgtaaacat gctgaacact ctggtgaaga tttcagatac catgatccag aaacgaacga
     4261 aaaatatatt ccatattgta tcgagccatc acttggtgca gatcgtgtaa cattagcttt
     4321 cttatgtgat gcatatgatg aagaaggcgt tgaaggtagt aaagatgcac gtacagtttt
     4381 acacttccat cctgcattag caccatataa agcagcgatt ttacctttaa gtaagaaatt
     4441 atctggcgaa gcgattaaga tttttgagca attaagttct aaattctcaa tcgatttcga
     4501 tgaatcacaa tctatcggta aaagataccg tcgtcaagat gaaatcggta caccttattg
     4561 tgtaacattc gactttgatt cattagaaga taatcaagtt acagtacgtg acagagattc
     4621 aatggaacaa gttcgtatgc caatctcaga gttagaagct ttcttaactg aaaaaacaaa
     4681 attctaatta aaaagacttt ccaccatatg tggtggctat ataatttata aaaagagata
     4741 ctggttagta aaattgccag tatctctttt ctattcaaaa aggtattcaa ataaacacat
     4801 ctatgtggct acttgaatac cttttattat tttatgttat tgttccaatc tttttaattg
     4861 gttgattagt ttctgacttt taaaaaacat acctgcatat tcacgatata acattaaaat
     4921 gatatctgac atttcatcaa taatttcttg atggatattc aatgaattca ttttatctat
     4981 cggtaatttt tgtaatacat ctaataaata tagtgtttta ttcgataata taactgcatg
     5041 tacatcttta gaagcctctt gccttgaaat cgcaccgtca aacttaaaac tataacctat
     5101 taaatctgct tgtgtgtcat taccactcac agcacagcga ttaaatgatg cagtaaaacc
     5161 aaatcgtttc atgcacttta acataactac gactgacatt aactgtgcag atgtacctga
     5221 ttctattttt tcaagaacaa attgtaataa ttgatagtta tatggtgcaa tgtcaccttc
     5281 atccattgag cgctcaatag tttcagctgc cagagcggca taactgctta cgtaaaggtc
     5341 catttgtaat ttataatgtt gactaataac atctacagaa tttaacgttc ccatacctcg
     5401 ccactgattg taaataaaca aaccataaac aaacaattgc gtttgcgctt gtaaacccgt
     5461 cttaactttt ttagcacgcc ttgccataag tggtactttt gcaccatgct catttaaaat
     5521 cgtgataatt ttatcagatt caccataatc aactgctttg atgataatcc ctttttggcg
     5581 cattaacaat tatcttcacc acttttaaga ttaatcttgg tcttcaacat aaccaatttg
     5641 gcgaataaag ttaactttgt ttcgccagtc tctttgaact ttgacccata attctaagta
     5701 aactttagag cctagaagca tttctatatc acgtctcgca cgttttccta cttcttttaa
     5761 ctttttaccg ccttttccaa tgacaattcc tttttgcgaa tctctttcaa catatatagt
     5821 tgcttcgata tgaacacgat cttcgctttc tttaaccata cggtccacat taacaccaat
     5881 cgcatgaggg atttcttcac ttgtaagatg aaggattttt tcacgaatga tttcacccac
     5941 tacaaattgt tcaggatggt ctgaaatttg atcatctgga taatatttag gtccttcggg
     6001 taaatacgtc tttaaaacat caataaaatg atcgacattt agcccttcta atgctgaaat
     6061 aggtacaatc tctgtaaagt ccatataact ttgatattct tcaatctttg gcattaattc
     6121 atctggatgc actaaatcta ttttatttaa tactaaaaat actggtgtct taacattttt
     6181 caacatttct ataatatatt catcgcctcg tccaatttcc tcattggcat taaccataaa
     6241 catgattgca tctatctcag ataatgtatt tttagcgact ttcatcatat agtcacctaa
     6301 tttgtgttta ggtttatgaa tacctggcgt atcaatgaat ataatttgcg cgtcatctct
     6361 tgtcataaca ccttgaattt tatttctagt tgtttgagct ttatcggaca tgattgctat
     6421 tttatggccg atcactctat taacaaatgt tgactttcct acatttggtc tacctataat
     6481 tgaaacaaat cctgatttat gttctgtcat ttattctaaa tcctttcctg aaaatccaaa
     6541 tggtagtaac tctgcgactg tcatcataac catatctcct ttatgatttg tcatatacac
     6601 aggcatatca tcatcacata attccttcaa aacttgacga catgcaccac aaggtgatga
     6661 cggtttatct gcatctacgg ttacagttat tgattcaaaa tcacctggtc tgtatccttg
     6721 agaaattgcc gataccaaac tagctcgttc agcacatatc gataatggat aagaagcatt
     6781 ttctacattg gtaccataaa aagttctacc gtctttcgtt tttaaataag cccctacttt
     6841 aaattgactg tatggcgaat atgattcttg ttgtgctttt ctaacttctt gaaaataatg
     6901 aggttgataa ctcatatatg cctccctaaa ataacgctat aaaatgtggt aaaaatacta
     6961 ttaaacctat aataaatgct aatattgaaa ctataagtac actaaaagcc gcaatatctt
     7021 tagcgtattt agctaaatca tgatattcaa cggtcactaa atcgacaaca tattcaatag
     7081 cagtgtttaa agcttcaaca gtgagaacta atgcaatagc aatgagtata aatatccact
     7141 caatccgatt aatatttagt acgagaccaa agacaatagc aacaatcatt gcaaacacat
     7201 gtaaaagaaa tttatagtct ttttgaatta agattttcag cccatcaagt gcatatttaa
     7261 accttttcat aattagtctc gtgttaatcc atatgcgttt aatattgtat cttgtcgacc
     7321 aaacatttcc ttttcatccg cttcagtcat atgatcataa cctaatagat gcaaaaatcc
     7381 atgtaatgct aaaaatccta attctcgttc aaaagaatgt ccgtaattgt ttgcttgttc
     7441 ttgcgctaca tccgtacaga taattatatc ccctaaaaca cgtggtatat caagaccact
     7501 aaaatcaatc tctggctcat cttcttctaa agcaaatgag attacatctg taactttatc
     7561 tttatctcta tatgttcgat taatttcttg tatttcttgt ttatctacaa atgtaacaga
     7621 aagctcagca tcgtcttcta tatgctcttc ttttttagca aattctaata aatcttcaat
     7681 ttgtttatac caagcatctt taactaagcc tgtgtgatcg ctaaaatcta tcgtaaacat
     7741 ttaattctct ccttcataat gttcaatgat cttacttacc aatggatgtc tgactacatc
     7801 gctctgatct aatttcaata tacttatacc tttaacgttg tgtaacctac tgaccgcttc
     7861 cttaagtcca cttttaacac ctttaggtaa atcgatttga gtttggtcac cagtaactac
     7921 catttttgag ccaaaaccta gtcttgttaa aaacattttc atttgcgcat gtgtcgtatt
     7981 ctgcgcctca tcaagaatta caaatgcatc ttctaatgtt cgtccgcgca tatatgcaag
     8041 tggcgctatt tcgataatgc ctctttcaat aaatcgctct gtttgttcac gcccaagaac
     8101 agtatataga ccatcatata aaggtcttaa atatggatct accttttctt tcaaatctcc
     8161 tggtaaaaat ccaagtgact ctcctgcttc aacagcaggt cttgttaata caatacgttt
     8221 aacagcacct ttacggagtt gctttgctgc ataaactaca gctaagaatg tcttacctgt
     8281 accagcagga cctataccaa atactaaatc attatttttc atggcattaa catatatacg
     8341 ttgccccatc gttttcgcac gaatcgtctt accaaatgca tctttagtta tctcttcatc
     8401 atataaatct aacagatgtt gaattgtgtt attatgcgcc attttaatag ctgcttcaac
     8461 atctttaatt gtaatattat tacctaaatc aataaccttc agcaaattga ttaatactga
     8521 ttccgctttt tctacgtttt ctatttttgt acctttaacg gcaacttctt gtcctcttgc
     8581 atggatgaca acatcgaaac tctcttcaat tgcttttaaa tgttcatcat tatttccaat
     8641 taaagcttga gattggttca tatcgtctat ttgtataatt ccaggcatac acgcgctcct
     8701 tttcatatat catttatatt cattctatga tgctttaata tctacttaat tatatcatgc
     8761 aattgattga tttaaacatc tttgcattga cttactcatt tttctttaat ttaaaataat
     8821 catttatact tttcaccttg tcataaaagc agattcaatt ttagactaat caattttttg
     8881 ctgtaattta aataactaat atttctcgaa aaattctctt tgttttttaa tttttcttaa
     8941 cgtaaaaaaa caagcaacta ccattacgat agtgcttgtt ttcaaaaaat tttataattg
     9001 ttttggttta gctaaaattt ctgaccatat cataccattg attacttcat ctttatcaaa
     9061 ttggaacgct gatttagtca aatctttttc gacattttga gaattaagca gctgctttaa
     9121 tttcaaacgt tttgtacgtt cagataagta tttatcctca ataatatctc tagcacgttt
     9181 ttccatttta gcaatttgct tttctttttc tctatcaatg tcagtacgaa tcgttttaat
     9241 atcatctcta agtgattttt ctaattgacg tctaatttcg tcagaattat cttgtctaga
     9301 aggtctatgt ttctgctcca ccggttctgc tttaggcact gtgattggtt tctctgtcat
     9361 cggttttgtt tcttgttgtt ttttaggtgc cataggttct acaacaggtt tcgatttagg
     9421 ctcttccttt ggaagttcat cgaataaagg tggtaacgta tcatcatatt ttcgtttcga
     9481 tgatttcttt tcttcttcat ttaattcttc acttatttct ttaaacgttc gctcaatttc
     9541 ttcaaaaaag ccaccttttt ttggttcatt atcggtagat gttttttgag gtggcttttg
     9601 attttgtcta tctttatgac tattttcgcg catagtagta atgatagaaa tgatcactga
     9661 tatgacaaaa attagaatac cgacactcat ttaatcacct ctcgacttaa tgttcaggtg
     9721 actcatcatc actttgatca gttcgtttat taattgcatt tctcatgcct gtatcagctt
     9781 cgatattttt caaattataa taatctttaa cactgatatt acctgaacgt aatgcttcag
     9841 ccatagctaa tggtacttca gattcggctt caactacttt agcatgcatt tcttgtacac
     9901 gcgctttcat ttcttgctca gttgctacag ccatagctct acgttcttca gcttttgctt
     9961 gtgcaatatt tttgtctgct aatgcttgtt cagtttgtaa gtctgcacca atatttttac
    10021 taatatcaac gtcagcaata tcaattgata aaatttcaaa tgcagtacct gaatctaaac
    10081 ctttgcttaa aactgtttta gaaatattat ctgggttttc aagtacttct gtatgatgct
    10141 tactagaacc aattgttgaa acgatacctt caccaacacg tgcgatgatt gtttcttcac
    10201 cagcaccacc aacaagtcga gcaatattag ctctaactgt gatacgagct ttggctttca
    10261 cttcaatacc gttcattgct acacctgcga taaatggtgt ttcaatgact ttaggattaa
    10321 cagacatttg aaccgcttct aatacgtcac gtcctgcaag gtcaattgca gcagcacgtt
    10381 cgaaaggaag atcaatgtca gcacgttgtg cagcaatatt agcgtcaaca actctgtcaa
    10441 catttcctcc tgctagataa tgcgattcta attggtttgt tgttaatgct agtcctgctt
    10501 tgtgcgcttt aattaatggc gctataactt ttcttggaga tacacgacgt aaacgcatac
    10561 caaccaatgt acctatacca acatgaacgc cagctgctaa cgctgaaatc cataaaccaa
    10621 tgggtacaaa tgagaataaa ataagtaatg caactactat aataactgct attacgataa
    10681 aacttaaact aaacatggta tcgctccttt taattaatct acttccctca caactactct
    10741 tgttccttca acttcaagga tttttaccgt tttattgcgc aaaataaagt tgccatctga
    10801 aacagcatca atacgttcat tttcacaaaa aataatccct gcaggtcgaa gatctgtaac
    10861 tgtttgagca gtctttccta cgaggtgcga gcggttatca tgagaattgt aacctgactc
    10921 agaattagtt gaatctttta agataacttt atccaaaaac ggaatctttc tgttgaaaat
    10981 cttcactaat atcacccatt ctacaatcgt taaaatcaag gcaacgataa cattcgcaag
    11041 cataaatagc aaattatcac cgagcgttgt tatgcttata gttatcagta tcatgccaat
    11101 aataccaatt actgcaccaa ctacaaataa ttcaattaca actaatataa cgccaattga
    11161 gaatattaag atagaatgca tattgacatt tccttggatt agaaatccca aaaataaaat
    11221 aagtaatgat aatgtggcga taataccagc tgcattgatt tttttagagt aaagttgata
    11281 tacaaatcct aagaatgtca aacaggttaa tattaacgta aaaataggtt gaacaattat
    11341 attgctaact tgttcaaccc aagtatctcc agccgttgat tccaagatag ttgtcatttg
    11401 taaaaaatta ttataactca caatttcacc tcgccctcac attaattata catgaaattg
    11461 aatatacttt atagttataa gaacctatag aatatcaatt tgattattta taaactatat
    11521 aacttttttt aattaaaaat tgacaacaaa aaaacatcac atattagtgt cagacactaa
    11581 cttgtgatgt ttgttttata taattaatat tcgtgttgag ggagtcaaca gaggtattaa
    11641 ttatttgaat ttacgtttac gtgcagcttc tgatttcttt ttacgtttta cgcttggttt
    11701 ttcgtaaaat tcacgtttac gtacttcttg gattgttcca cttttagaaa ctgaacgttt
    11761 aaatctacgt aacgcatctt caagtgattc atttttacgt actactgttt tagacatctg
    11821 tatttccctc cctccaaata tcaacggaac tttatagtca attgcacagt aaaactatgc
    11881 atttataagt ataatatatc tgtaatttat ggtcaattag taaattgttt tttatttgaa
    11941 acatatttca catcaaaatc acaaagactt ttagattttg ttctaaaaat ctcttaataa
    12001 tttatttaat gagaagagtt gcttatatag taaattgtga agccgttaaa acaacgttac
    12061 aaaacctata tctttaatac ggaaccatat ggtatgaatc aaggaatact taaactaaaa
    12121 cttctctatc agatttattt gttgcgaaat caacaacttt aattgcttgc ccttcattta
    12181 atggataatt tgcttgcgta attttaactt ttacaatttg acctatgagt gattcgtcac
    12241 cttcaaattg tactttcata taattatctg catatccaac taatgtacct tctgtgtcac
    12301 cctgttcctc aggaattact tcaagcacat cttgatcaaa tttagacgca tataactttc
    12361 cgagttgatt gcttagcgta attaacttat gcacccgttc atttttaatt tcttcatcaa
    12421 tttggtcatc cattcttgca gctggcgtgc caattctagg agaataaggg aaaacatgca
    12481 gttcagagaa cttatgcttt acgataaaat catatgtttc ttggaactca gcttcagttt
    12541 cacctgggaa accaacaatt acatcactcg taactgccaa gtctggtaaa gctttatgca
    12601 attttgttaa tcgttctgaa aatctatcca ttgtatactt acgtctcata cgttttaata
    12661 ctgtatctga accagattgt aatggaatat gcaaatgacg cacaactttt gttgaacgtt
    12721 ctaaaacgtc aattacttca tctgtaagtt gacttgcttc aattgaagaa attcgaattc
    12781 gttctaatcc attaatcgtt tcaagatcac gtaataattg ggccaagtta taatctttta
    12841 aatcttgacc atatccacct gtatgaattc ccgtcaatac aatttcctta tatcctgaat
    12901 tcactagttg cgtcgcttgt tcaactactt tttccggatc tcttgaacgc attaagccac
    12961 gagcccatgg aataatacag aatgtgcaga agttgttaca accttcttga atttttaatg
    13021 acgcacgtgt tctatctgta aaataaggga catctaattc ttcatattta cgatttttca
    13081 tgatatttcc aacaccatta attggttggc gttctttacg gaattcgtca atgtaaccta
    13141 atagtttatg tctatcttgt gtaccaacta ctacatcgac accaggaatt tccataattt
    13201 cagctgatga agtttgcgca taacaacctg ttacacagat tacagcatca ggattttgtc
    13261 ttattgcacg tctaattatt tgacgacttt ttttatcacc cgtattcgtt actgtacaag
    13321 tattaataac aaatacatca gcattcgctt caaagtcaac gcgctcatag tttgcttctt
    13381 taaataattg ccagattgct tcagtttcat aatggtttac tttacaacct aatgtgtgaa
    13441 acgcaactgt tgacataaat attcacccca ttaattcttt ttcataactt attgcactta
    13501 acgcatacaa tggcgcagtt tctgcccgta aaattctcgg cccaagacca acaactgtac
    13561 tagtattact aaataatgaa atttcatttt ctgacaaacc accctcagga ccaaaaatca
    13621 tcaacacttt atcctgagca ttgaattgtt gtaaagtttg cttgaaattg cttaactcac
    13681 catcttttgc ttcctcttca tatgcaataa gaatatagtc ataattatca atagtatcac
    13741 aaattaattt taaattcgac tcgaattgaa tagatggaat cactaaacga tagctttgtt
    13801 cagcagcttc tttaattatt ttttgccaac gctctatctt tttggcaact tttgcctcgt
    13861 ttaatttaac aattgaacgt tccatgctca cagctataaa tgatgaagca cccaattcag
    13921 tagctttttg tagcaaccac tcatatttgt cagctttgat tagtccactg caaatcgtaa
    13981 catcaactgg caattctgta ttaatatttt gtttttcttt taaatcaact tcaattttat
    14041 cacttgttat gtcagcaatt tcacataaat aaactgtttg atcattaaaa gttaaaataa
    14101 ttttactacc aacatcatat ctcattacat ttgttatatg atgaatatct tctttttttg
    14161 taataaaaaa acgctgactt acatcagcgt tttggtctat gaaataacgt tgcacattat
    14221 tcactcactt tctggccaac aagacaaacc caaccgttgt catgttgttc tgaaataatt
    14281 ttaaaaccta cacgctccat atgtgactgt ataccttcat acttctcttt tataatacca
    14341 gaagtaataa aataaccgcc ttcatttaga gtattataag catcttcaat catttcatca
    14401 ataatatgcg ctaaaatatt tgctattaca atatcaaatt tttctgtttc gtctttcaat
    14461 aagttacctg gaacagcttc aattaacgtt tcacaatgat ttcttctgaa gttttcttta
    14521 gctacactca ctgccatttc atcaatatcc aacgctttaa tacgttttac accgattaga
    14581 tgacttgcaa tacttaatat acctgagcca gtaccaacat caattactga atgctgtggc
    14641 aatacatatg tttctattgc cttcaaacac atacttgtag tcggatgatc acctgttcca
    14701 aaagccatac ctgggtcgag ctcaatgcaa agctcttcat ccgcttcttt agcatatgtt
    14761 tcccaactag gaactattgt gaacttcttc gacgctcgga atggatggaa atagtttttc
    14821 cattcatttt cccaatccgt ctctgcaata atttgctcac tgaattgaac gttatgttga
    14881 tcaagttcat ctaaatttaa taactcatct ttaatttgct gtcgcaactt atcatcataa
    14941 gtcatttcat taaaataggc tttcaatctt actcccttat ctggataatc ctcttttttc
    15001 aaagcgtaaa tttcaccgta tttatcttct ggttggttaa ttaaatcatc tgaatcttct
    15061 atcacgacac catttgatcc atgattttca agtatattgg tagccaattc tactgcttca
    15121 tgattaataa taattgaaag ctctgtccag ttcatacttt attctccctt aaagaatctt
    15181 tttgctctat ctttaaaatt cgaaggttgt tcattaattt cttcaccatt taattgggca
    15241 aattctttca ttagttcttt ttgtctatct gttaatttag taggcgttac tactttaata
    15301 tcaacatata aatctccgta tccatagcca tgaacatttt ttataccctt ttcttttaag
    15361 cggaattgct tacctgtttg tgtaccagca gggattgtta acataacttc attatttaat
    15421 gttggtattt ttatttcatc gcctaaagct gcttgtggga agctaacatt taatttgtaa
    15481 taaatatcat caccatcacg tttaaatgtt tcagatggtt taactctaaa tactacgtat
    15541 aaatcaccag caggtcctcc attcacgcct ggagagcctt caccagctaa tctaatttgt
    15601 tgttcattgt cgacaccttc aggtactttc acttctaatt taactgtttt attttcagta
    15661 ccttttccgt gacatgttgg acaagcttct tcaaattctt gaccacttcc attacattta
    15721 ggacaaactt gttcagtacg aactctacct aaaattgtgt tttgttctac agctacatga
    15781 ccagcgccat tacagtaact acaagtcttt ttacttgttc caggctttgc accatcacca
    15841 tgacatgttt cgcatgttac atctttacgg attgaaattt cttttgttgt accaaatacc
    15901 gcttcttcaa atgttaatgt cattgtatac tgaagatcat cacctttttg cggtgcattt
    15961 ggatctcttt gtctgccgcc accgaagaaa gagctaaaga tatcttcaaa accgccgcca
    16021 ccgaagccac taaaaccgcc aaagtcagag ccattgaatc cttgtccacc aaaaccttgt
    16081 ggaccatcat gtccaaattg atcatagctt gcgcgtttat tatcatcact taaaacttca
    16141 taggcttcag aaatttcttt aaacttttca tctgcacctt cttctttgtt aatatctgga
    16201 tgatattttt tcgaaagctt tcgatacgct tttttgattt catcttttga agcatcctta
    16261 ctaatgccta aaacttcata ataatctctt ttggccacag ctatctctcc ttttcttaat
    16321 taactcatat agtttaacgt aatatgtcat actatccaaa taaaaagcca aagccaatgt
    16381 tctattgact ttgacttttc agatcatgac aacattctaa ttgtattgtt taattatttt
    16441 ttgtcgtcgt cttttacttc tttaaattca gcatcttcta cagtactatc attgttttga
    16501 ccagcattag caccttgtgc ttgttgttgc tgttgagccg cttgctcata tacttttgct
    16561 gataattctt gaatcacttt ttcaagttct tcttttttag atttaatatc ttctatatct
    16621 tgaccttcta aagcagtttt aagagcgtct tttttctctt cagcagattt tttatcttct
    16681 tcaccgatat tttcgcctaa atcagttaaa gttttttcaa cttggaatac tagactgtca
    16741 gcttcgtttc ttaagtctac ttcttcacga cgttttttat ctgcttcagc gttaacttca
    16801 gcatctttta ccatacggtc gatttcttcg tctgataatg aagaacttga ttgaattgta
    16861 attctttgtt ctttatttgt acctaagtct tttgcagtta catttacaat accgttttta
    16921 tcgatatcaa acgttacttc aatttgaggt ttaccacgtt cagctggtgg aatatcagtc
    16981 aattggaatc taccaagtgt tttattatcc gcagccattg gacgttcacc ttgtaatacg
    17041 tgtacatcta ctgatggttg attatctact gctgttgaat agatttgaga tttagatgta
    17101 ggaatcgtag tgttacgttc aattaacgta ttcatacgtc cacctaaaat ttcaatacct
    17161 aaagatagtg gtgttacgtc taataatact acgtctttaa cgtcacctgt gataacgcca
    17221 ccttggattg cagctcccat tgccactact tcgtccgggt ttactccttt gttaggctct
    17281 ttaccgattt cttttttgac agcttcttgt actgctggaa tacgagttga tccaccaact
    17341 aagataactt catcgatatc tgagtttgtt aagccagcgt ctttcattgc ttggcgtgta
    17401 ggttccattg ttcttctaat taatgaatct gataattctt caaatttaga acgagttaag
    17461 tttacttcta agtgtaatgg accgttttca ccagctgaga taaatggtaa tgagatttga
    17521 gtttgtgata cacctgataa gtctttttta gctttttcag cagcatcttt caaacgttgt
    17581 aatgccattt tatcttgaga taagtctacg ccattttctt ttttgaattc tgcaactagg
    17641 tagtcaataa ttacttggtc aaaatcatca ccgccaagtt tgttgtcacc ggctgttgat
    17701 agtacttcga atacaccgtc acctaattct aggatagata cgtcaaatgt accgccacct
    17761 aagtcaaaaa caagaacttt ttcatcttta tcagttttgt ctaaaccata tgctaatgct
    17821 gcagctgttg gttcattaat gatacgctca acttctaaac cagcaatttt accagcatct
    17881 ttagttgctt gacgttcagc atcgttaaag tatgcaggta ctgtaattac agctttgtca
    17941 actttctcac ctaaatagct ttcagctgta ttttttaagt tttgtaaaat catagctgag
    18001 atttcttgtg gtgtgtatga tttaccttca atatctactt tataatcagt acccatatga
    18061 cgtttaatag attgaacagt gtttgggttt gtaatagctt gacgttttgc tacttcacca
    18121 acttgagttt ctccattttt gaaagctaca acagatggtg ttgtacgtga accttcaggg
    18181 ttttgaatta cttttggctc atcgccttct aatactgtta cacatgaatt tgttgtacct
    18241 aagtctatac caataatttt actcataata aaattcctcc atttaatcat taaattaatt
    18301 taattttaaa caatgtcttt tcgccaaatt taagttattg gtttactttg accattgatg
    18361 gtcttaatac tctatcttta agcttgtatc ctttttgtag ttcttgagtg atttcgccag
    18421 attcaaaatc agggttatca tcttgaacta cagcttggtg aatatttgga tcaaatgctt
    18481 caccttcagt tttaataact tcaagaccat tatcttttag tgcgttaatc aaactttcat
    18541 gcaccatttg tacacctttt tgaagagatt taaaagtctc atcatcacct tcaatttgaa
    18601 gtgcacgttc tatattgtct attgctggta aaatatctgt taacacacgt tgtgcttgat
    18661 atgttttgtt tatttcattt tctttttgaa ttctacgctt ataattttca aactcagcgt
    18721 agagccttaa atatttctct tcgttttcat ctgctaattg ttgaagttca ttaatttttt
    18781 gatcttttgg atctatttct tcaataacat tctcgtcaga cgtttcttct attgcttcat
    18841 cttgtaaatg acctttactt tcttcagctt gttcaactga atcatcaata ttttgtttga
    18901 cgtttgtttc ttcaactgtt gattcagtgt ttttttcaac tgattcgtct ttatttgtca
    18961 ttttctgtcc tccaatactt tctaatccat cattaccaaa ttctatttaa taattgaatg
    19021 acattttgat aatgcatagc tgtaggtcca atcacagcga tttgaccttt taacgtttca
    19081 tcaaaatgat attgacttgt tacaattgaa atatcactta agctgtcatc aatttcatta
    19141 ccaattttta cattaatatt tggtgaagat atatcttgta ataattctgc aattctattt
    19201 gattctatat attgtagaat gggctgaatt gaagatacat tactttcatt caatgcatca
    19261 ataagtttaa cctttccacc catataaatg ctattacttt gattagaaat atgattattc
    19321 atcgtattta acaatttatt gataaaaatt tcttcctgct ctgattgaac aaaagagaca
    19381 atatcatctt gtaaattctg attaaactca gttagtttgt ttgtaacaaa atttgatatt
    19441 gtatttagtt tgtcattatt aaacggtatg tctgaagcaa gatgtacatg ctcaacatga
    19501 cctgatgaaa atacgataac cattataact aaattaggat tagcacgaat caagtgtaca
    19561 ttattgataa tatcttgttt atgattagga tgaacaacta aagttgtata ttgagatata
    19621 tttgataatt catctgcaaa atatgtcaat gctgatgata catcatattg attctcaact
    19681 aacaattgat ttaatcgtct taatttattt gttttttgat gagatgtttg ttcaagtaaa
    19741 cgattgacat aatacctaaa acctaattgt gatggcgaac gccctgaaga actatgtgtc
    19801 ttctcgatat agtttaaatc ttcaagctgt ttcatctcat ttctaattgt agcaggacta
    19861 acattcaagt tatgtcgctc aattagtgtt ttagaaccaa cgggttgtcc aaaatcaaca
    19921 taatcctcaa caattgcgtt taatatactc aattgcctat ctgtaatcat gttttcacct
    19981 cattagcact cacttatctc aagtgctaat tataatttat caaattggtc aaagtaagtc
    20041 aatgttaaag actcgaaatt tcaatttttt taatcattta ttaggaaagc ttcaaaaacc
    20101 tcattaccta tgactttccc tctatttgta agtgcaatca catcgttctt ttctacaatt
    20161 aattccttct cttttaaatt atttattgtt tgaccaaaga cactttcaat agattggtca
    20221 aacttctttt tgaacctact actactcaca ccttcattta aacgcaaccc aagaaacatt
    20281 tcttcttcca ttctctcagt caaagaaggt ttatttgata ctaaaattgc tttactttct
    20341 ttatttatag ctttgatata atgattcact ggattgatat tcgtataacg cacaccatct
    20401 acataaccac ttgcacctgc tccaaatcca taatattcct cattaaacca gtaaacctta
    20461 ttatgttctg attcatggcc atctaatgca aaattagata tttcgtattg atggaaagga
    20521 gattgttcta tcttagacat cagcaactga tacatgtcag cacctaaatc ctcattagga
    20581 agtttgagca agccttttct atacatatta taaaattggg ttttaggttc aagtattaag
    20641 ccgtaactcg aaatatgttg aatatccata tctaaagcta gatctaaact ttgttcaaaa
    20701 tcttcaatcg tctgtttcgg taaatgatac attaaatcta aactgattga tttaatacct
    20761 gtgtttttag catttaacac cgaagtgtaa atatcttcag tattgtgcgt tctacctaaa
    20821 acagacaata actccggctt gaatgtttga acgcccattg aaatcctttt tactccatat
    20881 ttctctaata gttggacttt ctctttagtt aactcatcag gatttgcttc aaatgtatac
    20941 tcgcctgtga ttgtaaacgt atcacgtatt gctttaagta atctttccaa ctgattaata
    21001 gaaagggccg ttggtgtgcc gccacctaca tacatggtct ttaagatcct atattttgct
    21061 gtagacattt ctgtgattag tgcatctaag tactcatcta caggttgatt ctgtataaaa
    21121 tatttattga aatcacaata tgtacatatt cttacacaaa atggaatatg tatatatgca
    21181 ctttgtaccg tcattttaat gccccgcttc tcagactttt tatccaatcg gctatatcct
    21241 gttggactat ctgcaattat tcttactttt aactttaacg tttaatcaat gattgtctaa
    21301 tatatcgatt cattttaata tttattcttg tttagaggga gtgagataga aataataaag
    21361 aaccactaat gatttattat gtagtggctc ttacacatta gccagatgta atatgtcctt
    21421 aaaaataaaa ttacatgagt aaaactcatg cataagggag tgggacagaa atgatatttt
    21481 cgcaaaattt atttcgtcgt cccaccccaa cttgcattgc ctgtagaatt tcttttcgaa
    21541 attctctatg ttggggcccc accccaactt gcacattata gtaagctgac ttttcgtcag
    21601 cttctgtgtt ggggccccat tgttataagc tcattttaac tctgtcgatg gctgccaata
    21661 taacattgta aagcccagga cgttaattta tcctaggctc cctcttttta gttattaaac
    21721 tttattcatt gtaaattgtt aattgatttt ttaaaattat tcatcatcca ttttcaatac
    21781 agccaagaaa gcatcttgtg gaatttcaac attaccaact gctttcatct tagctttacc
    21841 tgctttttgt ttttcaagta atttacgttt acggcttatg tcaccgccat aacatttagc
    21901 taaaacgttt ttacccattg atttaatatt tgtacgcgct acaatttttt gtcctattgc
    21961 agcctgtaca ggtacttcaa attgctgtct tggaattaac gttttaagtt tttcaactaa
    22021 tgctttacca cgttcatatg caaaatctct atgaactgtg aagcttagcg catccacttt
    22081 atcaccattt aataaaatat ccatcttgac taaattactt tctttatttt cgatgaattc
    22141 ataatcaaat gatgcatatc ctttagtatt agatttaagt tgatcgaaga aatcaaatac
    22201 aacttcagct aaaggtaatt cataaacaat atttacacga atatcatcta aatagtccat
    22261 atttataaat tgtccacgtt tacgttgaca taattccatt actgcaccga catagtcatt
    22321 tggaaccatc atagttgcac gaacatatgg ctcaaatatt ttatcaattt tatcacgatc
    22381 tggcatttgt gctgggttat caaccgtcac ttctgaaccg tcccttaaaa cacattgata
    22441 aattacagat ggtgcagttg caattaattc aataccaaat tctctttcaa ttctttcttg
    22501 aattatttcc atgtgtaaca tacctaagaa accagttcta taaccaaaac ctaatgcttg
    22561 tgacgattca ggctcaaatt ctaatgatgc atcattcaat tgtaattttt ctaatgcttc
    22621 tcttaaatca ttataatttt tgttatctat tgggaacagt ccgcaatata ccattggatt
    22681 cattttctta taaccttgca atggttctga tgcaggtcta ctagctaatg tgatggtgtc
    22741 accaacccta gaatcatcaa catttttaat acttgcaata atataaccaa catcaccaac
    22801 tgttaattca tcaactggaa gctgcttagg tgtattaatt ccaacttctg ttacttcgaa
    22861 ctctttacca gtggccatca ttcgaatttt atctccggct ttaacaacac cgtccacaat
    22921 tcttatcgat gaaattaccc ctctatatgg atcatactca gaatcaaata ttaacgcttt
    22981 tagtggtgct tctgggtcac catctggagc tggcacaact tcaactattt tctctagtat
    23041 ctcttcaatt ccaatgttag atttagcact tgctaaaaca acatcgtctt ggtctaaacc
    23101 tatcatatct tcaatttctt gtttcacgcg ttcaggttct gcagcaggta aatcaatttt
    23161 gttaataaca ggcaataact ctaactcatt atctaatgct aaataaacat ttgctaatgt
    23221 ttgtgcttcg ataccttgag ccgcatctac tactaaaatc gcgccctcac aagctgccaa
    23281 agaacgtgac acttcatatg taaaatcgac gtgtccaggc gtatcgatta aatggaatgt
    23341 ataagtattt ccatctttag cttcgtactt taaacgtact gcgtttaatt tgattgtaat
    23401 accacgttct ctttctaaat ccattgaatc tagtaactga tcttgcatat ctcttgtttc
    23461 aactgatttg gtattttcta aaattctatc agccaatgta gattttccgt ggtcaatatg
    23521 tgctataatc gagaaattcc ttatattctc tcttcttttt aagcgttgct cattatccat
    23581 tttatccttc tcactttcat aaaataaacc cgtattatct ttcgcatctt tgatataata
    23641 acgtttttaa taggtaaatg caaacgccat aatcttggat gaactttgaa attataacaa
    23701 tctttattta aatacatact tgcgatatgt taatatgtta attacgtact tataattata
    23761 aaacgtattg attgtgtttc caacacaatt tattaaatct gcaaaatttt aagctaaccc
    23821 catcaaataa atgattgcac aacggttaga cttttgttaa aatatttctt gttgtaatca
    23881 aataaaattt tgataagatg aactcacttt taggaggtga cagaaatggc aaatatcaaa
    23941 tctgcaatta aacgtgtaaa aacaactgaa aaagctgaag cacgcaacat ttcacaaaag
    24001 agtgcaatgc gtacagcagt taaaaacgct aaaacagctg tttcaaataa cgctgataat
    24061 aaaaatgaat tagtaagctt agcagttaag ttagtagaca aagctgctca aagtaattta
    24121 atacattcaa acaaagctga ccgtattaaa tcacaattaa tgactgcaaa taaataatct
    24181 ttttaaataa aagttcaagc gcatgcttga acttttattt tttataaaga tagaatgaat
    24241 aattccagta ttaactgttt atccatatat gatgatttaa gtttataatc agtttccgca
    24301 caagcatcta taatattcaa taattcatca agttgataat gtcttacttg tcctaacgct
    24361 aattttactc tgtatggatg tactcctatt gttttagcaa tttgctgtcc actatatcct
    24421 ttttgactca gaatcttaca ttgataaaat aatcggtaat tacttgtaat tagtgcaagt
    24481 aatttaattg gttcttcttt catagttatt aaatctttta ctaaatgaat tgcttgttct
    24541 ttctttcttt tctgaatgta ttcagtcagt aaaaatacat tttgctctaa acttctatta
    24601 ataatttggt taacatcctg cttattaatt gttggtctat cgcctaaaaa taaaatcaac
    24661 ttttctatct cttgtgagac aatattaaag ttaatacctg tcaactcaat aaataaatct
    24721 aatgcatctc ttttgatatc tttgaaattc tcatttaatt tactttgaat ccattttttt
    24781 atttcttctt cagacatttg ctctattttt ttaagccttg catgcttttt tagagtttta
    24841 gttaactttt ttctttcatc aagtttattt tgatatatct caaagacaat caaattttcg
    24901 ccatcatatt tttcaataaa ttctattaat tgatctacat tatgagccat atcttttggc
    24961 gctttttcac ctgtaaatat atatgcattt ttaaccaaaa ttgctttttt atctgaaaag
    25021 aaaggcaatg ttaatgtttc ttcaacaatt ggtgcaatct ctgtttcgta taaattatat
    25081 ttcacaaagt taaagtcatc tctatcactt ttcaaaaatt gtgatatgat ttctgcactt
    25141 tgtttttcaa ccaattcagg cacatctcca taaatagcta caatattgtc gctcattcat
    25201 tacacccttt cattcgtttg acacatcaat agattatatc atgtttctgt atctataaac
    25261 cacttgcatt tccataagag cttgaatcaa cttttaaatt atcatctaag tcaattgtaa
    25321 cttgaccgtt ttgttgacta ttgtaaatgc gactgcgaat cctttgcaat cgtttaacaa
    25381 cttctatatt aggaagatga tacatattgt tcttcccaga agaaatcaaa cttattttag
    25441 gcttaatcat ctctataaat tctttagaac tacttgtctt gctcccatga tgtcctactt
    25501 ttaaaatatc aatctccggc aagttatatt tttttagtag taaagattca ttatttttac
    25561 tagcatcgcc cattaataaa acttttttat tttgatatgt aatcatagta ataatcgaat
    25621 actcattttt atctcggcta tttggaataa aactatcaaa aaatagaaaa ctactatctc
    25681 caagtttaaa actactaact tgtcttacat ccataagttt aatgttgtac ttatggctta
    25741 atttcgataa taacatcaat gtattactac tatatccctt attgtatatc actatatgtt
    25801 taattttaat atgactaata atatattcca cttcaccaat atggtcattg tgtggatgtg
    25861 ttaaaattag atactctaat tcatttatcc ctctttcatt tagcgttggt aaaatatgat
    25921 atttagaaat tgaataacta ggttgtttag tatcatcaat cactttccca cctgtatcaa
    25981 tcaagacatt ttggttctta ccgccttcat ataaaatact gtctccctgc cccacattta
    26041 acattgtaat tttgtgatgt gaatttgttg gaaacgttat taataatgtc agaataatta
    26101 tagtccaaaa cgtaaccaat atatatttac gtttagccaa taaccaaaat atgtaataaa
    26161 cagaaattat aaatactata aatatccaat cattaaactt gggaacagaa aaatgtgatt
    26221 gcttgattct tgtgaatagg tctagtaacc agtcatgaaa attaaaactt aggtcaacca
    26281 agtaatttag cggcgttaat cccacaataa aatgacttgt aataaagaat aaaatagata
    26341 gcggaaacaa tataatcgaa tagtacggta caaaaatcaa attagataaa aatcccaccc
    26401 attgaagttg atgaaagctt ggaatggcaa cgataaatga agctaattgt gcaataaacg
    26461 taattatgaa taatgattgt aactttgaca attgctgtaa aaaaggaaaa agtagcataa
    26521 taaaaaatga aatgatgaat gaaaattgaa atccaatatc ataaacaact agtggattta
    26581 aaataaacat aattataaat gcaaatgcta atagctgaat actctttatt ttaatttgct
    26641 tagtaataac cagtacaaga gttgtcatta ttatagctct tacagcacta ggtgcataat
    26701 ttgtgtattg agcaaataaa gctaatacaa tgattgtaat tcctttaatg acaaataaag
    26761 gtaaatttaa tcgttttaaa ggttggtaaa ttaaaaatac aattgcagct atatgcgagc
    26821 cactaactgc cagcaaatga tatataacta tctctttaac acgttcctta aattgctcat
    26881 taacttcttt tacgtcacca gtaatcaatg ccataatacg atccggaaac ttaatacccg
    26941 aatcataaat tcgattcatt ataaactgtt tatgtttctc aattaaatta gaccggttcg
    27001 attctaggca gctttgtaca actatacttt gtaatttaag agttacaaac ttattgtcat
    27061 taactttgaa ttgtccttta acaatacaat tacgactttc aattttcttt aaatcaaaat
    27121 tctttttatc tgttaaaaag aaaggatata tttcattttt taaactcaac ctacctttat
    27181 atgtgtcact accttgacgt tgaatttgga ttacttgagc acgctcatta aactgagaat
    27241 tacgttcaat ataattgata taattaaata ttgcttgttg tgaataatgt aaataccatg
    27301 cggagaaaat gattaaaaag agagaaatag gggcataaac gattttattt ttacgataag
    27361 taatatacaa aagtaaaatg aaaagaaatg tagagagcac tttgctagaa ttccaaagca
    27421 ctcctacaat cattgataac gcgacaaaca gcaaaacatt aacctttagt cagatattta
    27481 gcaacatatt ctggtgaaaa tggaattttt ttaaattcaa taccagattg tttgagtaat
    27541 ttagttgcat attcatggtt atgataatct tctgcatagt agatacgctt tatacctgct
    27601 tgaataattg actttgtaca atttaggcat ggaaaatgag taacatagat tgttgcacct
    27661 tcagtagata caccttgttt tgcacattgt aataaagcat tcatttctgc atgtatcgtt
    27721 ctgatacaat gtccatcttc aattaaacat ccttcatcta tacaatgcac ctcgccagct
    27781 acagagccat tataaccacc agcaataata cgattatcct taacaatcgt tgcacctaca
    27841 gataatcttt gacaagttga acgtaatgct agcaaatgac tttgtgccat aaaatattct
    27901 tcccatttga ttctttccaa gatgttcacc ttccatactt aaaatttagt aacattttct
    27961 aataatataa gattaatcac aaaaaataaa atttgcaatt aaaataatcc attatgtcgt
    28021 gaaataagat ttcagtttat caaaagtttt acttccaaaa ccttttactt ttttcaaatc
    28081 gtcaatttct tgaaatgcac cttgttggtt gcgatattca acaattgcat tagctttagc
    28141 ttgcccaact ccaggaacag acatcaattc tgatacagat gccgtattta aatttacttt
    28201 agtattattt gtgttcccat ttttttcgtg cacactgttt acttcaattt gtggttcaac
    28261 attcttttgt cctttatgag gtatgaaaat cattttttga tctgttaatt tttcagacaa
    28321 attaatttga cttacatctg catcctccaa taattgtgct ttatcaagta aatcaactac
    28381 tctatcctta gatgtcattt tataaacatt aggatgttta acagcacctt ttacatcgac
    28441 atatacagga cccttatttt tggaattatc tccatctttg acctggacat cttctacttt
    28501 ggacaaacta ctattttcac tagtgctttg tttcagagca gtatctttat tttcaaaatt
    28561 tcttgaagta taatcatctt gtctccagaa tataaaacca attaatacca taattaaaac
    28621 aacagcactt agaatatata acttccattg agttaaaaaa tctttatagc gtaataaaaa
    28681 ttgatacaat aaaaccactc ctcaacttta tacacgtctg agcgagtggt tttactttta
    28741 ttttttcgca atgaaaaaca atctttctgc atcttcatta tgttcatcta tattaaaatc
    28801 agtaaatgtt tcaacatgtt taaaaccaac ttgagctaac caagacaaat atgtcttttc
    28861 atcaaatgtt ctctgaaaat gagactcatc aaatcttgaa tatgtttcat cttcatgtcg
    28921 aatgaaaaat gtcata
//
LOCUS       DANJFZ010000025        28909 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.025, whole genome shotgun sequence.
ACCESSION   DANJFZ010000025 DANJFZ010000000
VERSION     DANJFZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.025"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..252)
                     /locus_tag="PWN45_000932"
     CDS             complement(<1..252)
                     /locus_tag="PWN45_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0622715.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYED"
     gene            complement(313..1404)
                     /locus_tag="PWN45_000933"
     CDS             complement(313..1404)
                     /locus_tag="PWN45_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0622716.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(1579..1881)
                     /locus_tag="PWN45_000934"
     CDS             complement(1579..1881)
                     /locus_tag="PWN45_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622717.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(1895..2215)
                     /locus_tag="PWN45_000935"
     CDS             complement(1895..2215)
                     /locus_tag="PWN45_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HDL0622718.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(2366..2650)
                     /locus_tag="PWN45_000936"
     CDS             complement(2366..2650)
                     /locus_tag="PWN45_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622719.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(2659..3087)
                     /locus_tag="PWN45_000937"
                     /pseudo
     CDS             complement(2659..3087)
                     /locus_tag="PWN45_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(3282..3506)
                     /locus_tag="PWN45_000938"
     CDS             complement(3282..3506)
                     /locus_tag="PWN45_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDL0622720.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(3523..3726)
                     /locus_tag="PWN45_000939"
     CDS             complement(3523..3726)
                     /locus_tag="PWN45_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDL0622721.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            3885..4445
                     /locus_tag="PWN45_000940"
     CDS             3885..4445
                     /locus_tag="PWN45_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0622722.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            4464..6866
                     /gene="mnhA2"
                     /locus_tag="PWN45_000941"
     CDS             4464..6866
                     /gene="mnhA2"
                     /locus_tag="PWN45_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDL0622723.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            6853..7278
                     /gene="mnhB2"
                     /locus_tag="PWN45_000942"
     CDS             6853..7278
                     /gene="mnhB2"
                     /locus_tag="PWN45_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDL0622724.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            7275..7619
                     /gene="mnhC2"
                     /locus_tag="PWN45_000943"
     CDS             7275..7619
                     /gene="mnhC2"
                     /locus_tag="PWN45_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDL0622725.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            7609..9105
                     /gene="mnhD2"
                     /locus_tag="PWN45_000944"
     CDS             7609..9105
                     /gene="mnhD2"
                     /locus_tag="PWN45_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDL0622726.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            9106..9588
                     /gene="mnhE2"
                     /locus_tag="PWN45_000945"
     CDS             9106..9588
                     /gene="mnhE2"
                     /locus_tag="PWN45_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDL0622727.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            9585..9887
                     /gene="mnhF2"
                     /locus_tag="PWN45_000946"
     CDS             9585..9887
                     /gene="mnhF2"
                     /locus_tag="PWN45_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDL0622728.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            9862..10299
                     /gene="mnhG2"
                     /locus_tag="PWN45_000947"
     CDS             9862..10299
                     /gene="mnhG2"
                     /locus_tag="PWN45_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDL0622729.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            10639..12681
                     /locus_tag="PWN45_000948"
     CDS             10639..12681
                     /locus_tag="PWN45_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL0622730.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(13841..14770)
                     /locus_tag="PWN45_000949"
     CDS             complement(13841..14770)
                     /locus_tag="PWN45_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0622731.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(14767..15603)
                     /locus_tag="PWN45_000950"
     CDS             complement(14767..15603)
                     /locus_tag="PWN45_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0622732.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(15597..16340)
                     /locus_tag="PWN45_000951"
     CDS             complement(15597..16340)
                     /locus_tag="PWN45_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0622733.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            16462..17106
                     /locus_tag="PWN45_000952"
     CDS             16462..17106
                     /locus_tag="PWN45_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL0622734.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(17186..17938)
                     /locus_tag="PWN45_000953"
     CDS             complement(17186..17938)
                     /locus_tag="PWN45_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDL0622735.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            18174..18938
                     /locus_tag="PWN45_000954"
     CDS             18174..18938
                     /locus_tag="PWN45_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDL0622736.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(18999..19793)
                     /gene="tagH"
                     /locus_tag="PWN45_000955"
     CDS             complement(18999..19793)
                     /gene="tagH"
                     /locus_tag="PWN45_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDL0622737.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            20140..20952
                     /gene="tagG"
                     /locus_tag="PWN45_000956"
     CDS             20140..20952
                     /gene="tagG"
                     /locus_tag="PWN45_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDL0622738.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            21051..22154
                     /locus_tag="PWN45_000957"
     CDS             21051..22154
                     /locus_tag="PWN45_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL0622739.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            22151..23212
                     /locus_tag="PWN45_000958"
     CDS             22151..23212
                     /locus_tag="PWN45_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0622740.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAIHVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            23275..23673
                     /gene="tagD"
                     /locus_tag="PWN45_000959"
     CDS             23275..23673
                     /gene="tagD"
                     /locus_tag="PWN45_000959"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDL0622741.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(23790..25085)
                     /gene="pbp4"
                     /locus_tag="PWN45_000960"
     CDS             complement(23790..25085)
                     /gene="pbp4"
                     /locus_tag="PWN45_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDL0622742.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            25506..27233
                     /locus_tag="PWN45_000961"
     CDS             25506..27233
                     /locus_tag="PWN45_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622743.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            27592..28821
                     /locus_tag="PWN45_000962"
     CDS             27592..28821
                     /locus_tag="PWN45_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0622744.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
BASE COUNT         9671 a         4303 c         4868 g        10067 t
ORIGIN      
        1 atcttcatac tttttttgaa tcatattcgt tttattaact gcttctttac tgtcattatt
       61 tgctcttgta gctttaataa aagaagctaa taaaagaatt aacaaaatag ctaataatga
      121 gtaaaaacaa attacaatca tcttacgacg atttttaaat tttggaatta ctcggcgttg
      181 ttctttagga atatcaaaat gctttccgtt tttagaaaat cgtgttaaaa tgtacttgct
      241 tattattttc ataaacgatt ttctaaaatc ctttacgaaa tttttaataa aaatcacatc
      301 ccagttattt ttttaacact tatcttttga taagtgcttt ttttgtttta aagcctccaa
      361 ataatgatta attctattcg tatgctcatc attaaattgt gaatgcgcta aaattttatc
      421 taaaaaatca gtatctgtaa gcaacccctc agattgcgcc atactttcgg cagttaacac
      481 tgtatctaaa gtagtagcaa cttgtttaca taaccaatca agattatcct caatggactt
      541 taatgttggt ctagtagtta aattcaattt ttcagtatct ttaataaata acgcccaagg
      601 tctatataat ggccaccgct tacgattttt atcatttcca cctttaggag gagagataaa
      661 acgcaaatta ttatgcaata tagactttac aatttcgctt atattatctg ttctactcaa
      721 cactttcgca caattaactg catatgcctt tcgcatttgt atttcataac gattccatag
      781 cccaatgtct tcgacatcac aatgacgttt aaacgcttgt tcataattct tttcataaaa
      841 acgacaatat aaattgcttt gtttagaacc aatataaaaa gtagcgcctt tacttcttcc
      901 gtctgacaaa ttaaaaccgt ttattgcgct tccagctcta aaacgtgaaa cacactcagc
      961 tttttcagcc tttttaatta ataaaggcac ttttaaatat gttttataat catcaattgc
     1021 aatatcaatt cttgtaaaat ttgcattaac tgataaacat ttgtcaaaaa aatcttgcca
     1081 agttttattc aactgctgta aatgatgttc aaactctcta caacctgtac cagttaactg
     1141 taagtgaatg cccattgatt catcatcact tgaataatac aatttaatgt ttgaaaattc
     1201 ataaccgcga ttataaaagt aaatgccact atttcgtttt ttgaacaacg ttataggcaa
     1261 accgataacg tcctcaatta ctgctgaaat aggggctata tggaaagtga cctgaaccca
     1321 gtcaacaact gcctccacag cacttttatt tgtacgctct acccccctat tagtaagggg
     1381 ggtacactgg ttttttaaag tcatatttat ttatgcctcc tatcaaatcg caagcgattt
     1441 gatacatgac cgttctcgct tttttaacgc gtatttgtcg caggctccgc ccaaatgctt
     1501 aacgcactcc gtgcttgcat ttctgcgcct gcatacgcta aaagctacac ggtcatgtta
     1561 aatcactaat actatatgtt aaacatcaat atcgccaata taaatacaat cttccaaatt
     1621 cacttgtagc attctgccat gtcctgcttc actcaagctc atacacaata gacaaaggtc
     1681 agcgtaaatt ttaggccgac cttttaccgt attttcttta acgtaactat cattaaattt
     1741 agactgtatt tctttagcta aagtcattaa tgaaacttta tcgccccaat ttatacgact
     1801 agcaacatag tttaaaatag gttcactcaa atttgaacta tttgtgtagc gatataatct
     1861 atctgctgta taataatcca tttacatcac ctaattattt ttgtttaagt gcactattag
     1921 aaccaactac tttaatatct tctgcattcc agctatatgc gatagttcca aaatcgcctg
     1981 acatttgaac atacatttta acgtctggtt caactaattc gcattcttgt aaatagtcta
     2041 attccacttt attaggcgtt ttaacagtga tttgttcgcc ttgttcttga ctcgctaatt
     2101 tatatgcata aacagtttta tcagtacgct ctccattttc atatttatat ttttcatcta
     2161 cacctaaaaa gtttaattgt cctaaagttt ttttaatatc caatactggt ttcataatat
     2221 atccatcctt tacaaaattt ttaataaaat taaacgactt ctaaagggtc gcacgaatag
     2281 gaataactcc cgcaaatccg taccactgca tttttgataa tggagtgtct atgcttctcc
     2341 tgacctatca tatttttaat caaacctatc ggttcatcac ctcaaaaaat tctaagtctt
     2401 caattgaaat atgatacatc ttacaaataa atgtattaga cacaccatat tgttttaaaa
     2461 atttataatc cttataagta aatcgataat cattcttttt tgccatttca caaacttcta
     2521 atatacgaaa atatgcaaaa ttatacattt tacaaatttt tcttttagaa acaccatgaa
     2581 atcttaaaat tacaaattta agataattta attgatttga accatcaaaa aaaggtgtaa
     2641 actcagtcat aattatcaat tcctttcata agaattaact gttaactaaa gtttacacca
     2701 ccaattaaaa tcatgaattg tagcgccaaa taatccaaat gtatcaataa tgacacccat
     2761 aataatttga cccgcaactg ttgcaattac agttaatgtt gcacctaatt ttggcaataa
     2821 caataaattg ccagttaaaa agctaacccc aagcaaacca ccgactaccc atgtgtagtt
     2881 aaatgattga ttattgtaaa agtgaatagt aaatacttct ggattgataa tgatatttaa
     2941 aataattaaa caaattgttc caactgaaaa tgaaatgaat gaagtataga aaggcgattt
     3001 agtatacagt gatagtcttg aattgacaga tgtttggata ggaataagca ttccaactag
     3061 gacaccaatg atatagaaaa gaagcatatg aataaagtct cactttccaa agttaataat
     3121 aaaatcatat atttacatta tcattataga gaaaatatac aactcattta caataatgtt
     3181 gaaaaaaaca ggacattatt acaacaaaat caccctagac aatcatgatg aaagtctagg
     3241 gtgttagtat ttatactaaa ttaattgcgc tttctttata tctatttctt atcttcaaat
     3301 aaaacagttg ttgttttatc aataatttga gcagaatgta ctttggtgat agtaccacta
     3361 tttgacttta taatatttaa atctactaat gcattcatac tatcacgtgc aacttgctcg
     3421 gtcactacgt tgtttaattg tggtaattgc agttttacag gcttgtctag tgaagattta
     3481 aaacttaatt caagtgtttt agtcatgaat atgtcctcct gattaaattg ataaagattt
     3541 gatgagttcg atattgctat atgtttctcc agtaagtcgc tcaataagtt tgctgaatgt
     3601 tttaatttgg tcgtttgatg catcagggtt aatgttagcg aatcgacgct taaattctgt
     3661 ttgtttgccg ttagcgtcta ctttagtaaa tgataataca atagtgatgt ggtttatttt
     3721 actcatattt taaaacctcc tttcacacta tatatcgaaa caaaataata aaatggctaa
     3781 ttttattttc tatgtttaaa atctataaaa aaggcaatag atatatgtaa ctaaaatata
     3841 atatagctat gattaagaca cttaaagcaa ggggaggttg agtaatgaat aaagtagaag
     3901 cgattaaatt taatgatgat attgttaaaa tgtatgaagc gctcaagata aaatctgaac
     3961 gtgactattt attctttaag ttagctatac atagtggatt gaaagtatca gaattattaa
     4021 caattacagt ctctcaagtt aagagactaa ttgaaaagtg tacgttatca gaaatgtgta
     4081 aagcacattt tcattcgttg attaaaatta ggttaccaga aacattatcg aaagaactac
     4141 ttcaatatat agaggacagg agtctttcga atgaagacgt tctttttcaa tcactacgaa
     4201 caaatcaagt attatctaga cagcaagcat atcgaataat tcaccaagca tcaattgaag
     4261 ctggtataga taatgtagga ctaacgacat tgcgtaagac atttgcatat catgcttatc
     4321 aaaaaggtat acctatacca gtcattcaaa agtatttagg gcatcaatct gctattgaaa
     4381 cactaaattt tatcggttta gaaaatgagt gtgaacatag tatttatatt tcattacaat
     4441 tatagaaaca aaggaggcta ataatgagtt tggtttattt attaattgct atacttgtga
     4501 ttatggcgat gatacttcta atgtctaaac gtagagcatt ggctaaatat gccgggtaca
     4561 tagcgttggt tgcacctgta atttcatcta tctatttttt gattcaaata ccatcagtag
     4621 ctaaactgca atatctttct acctctattc catggattaa gacattagat attaatttag
     4681 atttacgttt agatggttta agtttaatgt tttctcttat tatttcactt attggaattg
     4741 cagtattctt ctatgcaact caatatttat cctctcgaaa agacaattta ccaaggtttt
     4801 atttttattt aacgttattt atgttcagta tgattggtat tgtattatca gacaatacga
     4861 tattgatgta cattttttgg gaattaacga gtgtatcatc atttttattg atttcatatt
     4921 ggtataacaa tggtgacagt caatttggtg cgattcaatc atttatgatt acagtatttg
     4981 gtggattggc gttattagtt ggttttatta tgctgtatat catgacagga acgaataaca
     5041 tcacagagat attaggacaa gcagatcata ttaagaatca tggattgttt atccctatga
     5101 tttttatgtt tttattaggt gcatttacaa aatcagcaca atttccattt catatttggc
     5161 tacctagagc aatggctgca cctacacctg taagtgctta tttacattca gccacgatgg
     5221 taaaagctgg tatcttttta ttacttcgat ttacaccatt attaggtctt agcaatatgt
     5281 acgtatatat cgttacgttt gttggtttaa taacaatgtt atttggttca attacagctt
     5341 taaaacaatg ggatttaaaa ggtatcctag cgtactctac aatcagtcaa cttgggatga
     5401 ttatggctat ggtgggtata ggtggcggat atgctcaaca ccaacaagac gcaatagcat
     5461 ctatttatgt atttgtatta tttggtgcgc tatttcatct aatgaatcat gccatcttta
     5521 aatgtgcgct tttcatggga gtaggtattt tagatcatga agcaggttca agggatatac
     5581 gaattttaag tggaatgcgt caactatttc ctaaaatgaa tctagtcatg acgatagcgg
     5641 ctctatctat ggctggagta ccatttttaa atggattttt aagtaaagaa atgtttttag
     5701 atgcattaac acaaactgga caattatccc aatttagttt gatttcaatg atagctatcg
     5761 tgtttgttgg tgttattgcg agtgttttta cattcacata tgcactatac atggtaaaag
     5821 aagtattttg gacaaaatat gattctaagg tttttactaa aaaaaatatc cacgaaccat
     5881 ggttgtttag tttaccatct cttatattaa tggtgctagt acctgtaatc ttttttgtac
     5941 caaatatatt tgggaagggg attatcgttc tagcattaag agctgtatca ggtggtaatc
     6001 atcaaattga tcaattggca ccacatgttt cgcaatggca tggatttaac ataccgcttc
     6061 ttttaaccat catcattatt ttattgggta gtgtactagc aatcaaagta gattggaaaa
     6121 aagtgttcac aggtaaaatt agacagattt cagtttcaaa aagctatgag atggtatatc
     6181 gacattttga aaagtttgct acgaagcgat ttaaacgtgt tatgcaagat cgtttaaacc
     6241 aatacattat tatgacctta ggcatattta tgattatcat tggatatggt tatattcgaa
     6301 ttggacttcc taaagtacat cagttacatg tttctgaatt tggggcatta gaaattatat
     6361 tagcaatcgt aactgtcaca attggtattt ctttaatttt tatacgtcaa cgactgacaa
     6421 tggtcatttt aaatggagtc atcggatttg ttgtgacctt attctttata gcaatgaaag
     6481 cccctgatct agcattgact cagctagtag ttgaaacaat aacgacgata ctatttattg
     6541 tcagtttttc aagattacca aacgtgccaa gatctaacgc taacaaaaaa agagaaataa
     6601 ttaaaatttc tgtatcactc ttgatggcac ttattgttgt atcattaatt tttattacac
     6661 aacaaacaga tggtttatca tcaatatcag acttttattt aaaagctgac aaactaacag
     6721 gtggtaaaaa tattgtaaat gcgatacttg gtgactttag agcattagat acattatttg
     6781 aaggattagt gttaattatt actgggctag gtatttacac attattaaat tatcaagatc
     6841 ggaggggaca agatgaaaga gaatgatgtc gtgttaagaa cggtcacgaa acttgttgta
     6901 tttattttat tgactttcgg attctatgtc ttcttcgcag gtcataataa tcctggtggt
     6961 gggtttattg gtggtttaat atttagttca gcgtttattt taatgtttct ggcttttaat
     7021 gttgaagagg ttttagaaag tttaccgatt gattttagaa ttttaatgat tattggagca
     7081 ttggtatcat ctattactgc gataatacct atgttttttg gaaaaccatt tttgtctcaa
     7141 tatgaaacaa cttggatact tccaatttta ggacaaattc atgtaagtac aataacactt
     7201 tttgaattag gtattttatt ctcagttgtt ggtgttattg tcacagtgat gttgtcgctt
     7261 agcggaggtc gatcatgaat ttaatattat tactagttat aggattttta gtgtttatag
     7321 gaacatatat gattttatca atcaatttaa ttcgtattgt aatcggaatt tcaatatata
     7381 ctcatgctgg taatctcatt attatgagta tgggaacgta tggttctagt agatcagaac
     7441 cactaataac tggtggaaac caattgtttg ttgatccctt gttacaagct attgtactaa
     7501 ctgcaatagt tatagggttt gggatgactg cgtttttact tgtacttgtt tatagaactt
     7561 ataaagtaac aaaagaagat gaaattgaag gcctaagggg ggaagatgat gctaagtaac
     7621 ttattgattt taccaatgtt attaccattc ctttgtgcct taatccttgt atttttaaaa
     7681 aataatgatc gtatttctaa atatttatac ttaggtacaa tgactatcac cacaattatt
     7741 tcattaatgc tattaattta tgttcagcgt caccgtccaa ttacgctaga ctttggagga
     7801 tggtcagcgc cctttggtat acagttttta ggagattctt taagtttaat tatggttaca
     7861 accgcttcgt ttgtgattac tttaattatg gcatacggat ttgggcgtgg cgaacataaa
     7921 gcaaatcgtt atcacttgcc atcgttcata ttatttttaa gtgttggcgt gataggctct
     7981 tttctaacat cagatttatt taatttatac gtcatgtttg aaattatgtt actagcgtca
     8041 tttgtactca ttacacttgg acaatctgta gaacaattac gtgctgcaat tatttatgtt
     8101 gtcttgaata ttattggttc atggctattc ttattaggta taggtttact ttataaaaca
     8161 gtaggtacat taaacttttc acatattgca atgcgtttga atgacatggg agataatcgc
     8221 actgttacaa tgatttcatt aatcttctta gtcgcattta gtgcgaaagc agcgctggtc
     8281 ctttttatgt ggctacccaa agcctacgct gtgttaaata ctgagcttgc agcattattt
     8341 gcagcgttaa tgaccaaagt aggggcctat gcattaattc gattcttcac tttactattt
     8401 gatcaacata atgatctcat acatccattg ctagcaacta tggctgctat aactatggtc
     8461 atcggcgcta taggtgtcat tgcttataaa gatattaaaa agattgcagc ttaccaagtc
     8521 ataatctcaa taggatttat cattttaggt ttaggaacaa acacgtttgc aggtattaat
     8581 ggtgcaatat tttatttggt aaatgacatt gttgtaaaaa cattgctatt ttttattatt
     8641 ggtagtttag tttacattac aggctatcga caatatcaat atttgaatgg cttagctaaa
     8701 aaagaacctt tatttggagt tgcgtttatt ataatgattt ttgctattgg cggcgtgcct
     8761 ccatttagtg gctttccggg gaaagtactt attttccaag gtgcattgca aaatggcaat
     8821 tatattggac tagcgttaat gattattact agtctaattg caatgtacag tttatttagg
     8881 atactttttt atatgtattt tggagataaa gatggggagg aagttaattt taagaaaatc
     8941 ccgctatatc gaaaaagaat tttaagtatt ttagtagttg tggttatcgc aatcggaatt
     9001 gctgcacctg ttgtgttaaa tgttacaagt gatgcaactg agttgaacac gagtgatcaa
     9061 ttatatcaaa aacttgtaaa tccgcatttg aaaggagagg actaaatgaa tcaaatagtt
     9121 ttaaatatta tcattgcatt cttatgggta ttatttcaag atgaagatca ttttaaattc
     9181 tcgactttct tttctggata tctaattggt ttaattgtca tttatatatt acacaggttt
     9241 ttcagcgatg atttttatgt tagaaaaata tgggtagcta ttaaattttt aggtgtttat
     9301 ttatatcaat taataacatc tagcattagc acgattaatt atattctttt taaaacaaaa
     9361 gatatgaacc ctggattact ttcatatgaa acaagactaa caagtgattg gtcaataaca
     9421 tttttaacaa ttttaattat tataactcca gggtctacag taatacgaat ttctcaagac
     9481 tctaaaaagt tttttattca tagtatcgac gtgtcagaaa aagaaaaaga tagtttgtta
     9541 agaagtatta agcattatga agacttaata ttggaggtgt cgcgatgata caaacaataa
     9601 cacatattat gattattagt tcactcatta tttttggaat tgcattaatc atctgtttat
     9661 ttagattaat caagggacct acaacagcag atcgtgtcgt tacatttgat acaacaagtg
     9721 ctgtcgtaat gtcaattgtg ggtgtgttaa gtgtacttat gggcaccgtt tctttcttag
     9781 attcaatcat gctcattgcc attatatctt ttgtaagttc tgtttcaata tcacgcttta
     9841 ttggtggggg gcatgtgttt aatggaaata acaaaagaaa tctttagtct tattgctgct
     9901 gtgatgttgt tgttaggtag ttttattgct cttattagtg caataggtat cgtgaaattc
     9961 caagatgttt tcttaagaag tcacgctgcg acaaaaagtt caactttatc cgtgttatta
    10021 actttaatcg gtgtattaat ttattttatt gtgaatacag gatttttcag tgtgcgttta
    10081 ttactgtcac ttgtttttat taatttaact tcaccagtcg gcatgcactt agtcgctcgc
    10141 gctgcttatc gcaacggcgc ttatatgtat cgaaaaaatg atgctcacac acatgcatca
    10201 atattattaa gttcaaatga acaaaactct acagaagcat tacaattacg tgctgaaaaa
    10261 cgagaagagc atcgtaagaa atggtatcaa aacgattgaa gtgttggcta aattgttaag
    10321 cataacaatg cttttgaaaa tctgttttca aaattaatca ttaaaagagt actttgaaat
    10381 tggttagtcg attttaaggt gctcattttt tgtattataa gttaatgttg ttatgtgaaa
    10441 gcaagttgtt tatttataca aacaattagt gataaaataa aattaaataa ttgcaaattt
    10501 aagtactgca atcgtagaca tatatctata ttaaagtgct tttgtggtcg ctaactcaat
    10561 ttatcctcta aagtataatt acaacgagat gtgatactta attttcaatt aaacaatact
    10621 ttttagagag gtgaaagttt ggaaatattt gaaacaattc ttatatttat agctgttgtg
    10681 atactaagtt cgtttgtcca tactttcata cctaaagtac ccctagcatt tatacaaatt
    10741 ttcttgggca tgttactatt tattacccca atccctgttc aatttaattt tgattctgaa
    10801 ttgtttatgg taacaatgat tgcgcctttg ttatttgtag aaggtgttaa tgtttctaga
    10861 gtccatttaa ggaaatatat taagccagtg atgatgatgg cattaggatt agtcattact
    10921 actgtgatag gtgtaggttt atttattcat tggatttggc cagatttacc tattggagca
    10981 gcatttgcaa ttgctgccat tctttgtcct actgatgcag tagcagtgca agcaatcact
    11041 aaaggaaagg tcttgccaaa aggagcaatg acaattcttg aaggtgagtc attattgaat
    11101 gatgctgctg gtattatttc atttaaaata gctgttggag tattagttac aggtgctttt
    11161 tcacttgttg atgctgttca gttgttttta attgcatcaa ttggtggcgc agtggttggt
    11221 ttacttatag gtatggcatt agtaaggttc cgattaacat tgatgcgtcg aggatatgaa
    11281 aacattaata tgtttacaat tattcaattg ttaacaccat ttgttacgta tttaattgct
    11341 gaattgtttc acgcatcagg aatcattgca gcagtagttg caggacttgt acatggtttc
    11401 gaacgtgaca gaattatgca agtacgtaca caactgcaaa tgagttacaa tcatacatgg
    11461 aatatactag gttatgtttt aaatggcttt gttttttcaa tattaggatt tttagtacct
    11521 gaagttatta ttaaaattat caaaacagaa ccgcacaatt taatcttttt aataggcatc
    11581 actattgttg ttgctttagc tgtctatcta tttagatttg tttgggttta tgtcttatat
    11641 ccttattttt atttagccat cagtccattc caaaaaatga tgactaaaaa tgatgatgat
    11701 aatccaacga ctgagaaacc accaaagcga agtttatacg ctttaattat gacgttatgt
    11761 ggtgtgcatg gaacaatttc tttagcaatc gcattaacgt taccgtattt tttagcaggg
    11821 catcatgctt ttacgtatag aaacgactta ttatttattg catctggtat ggttattatt
    11881 agtttggtag ttgcgcaagt attattgcca ttattaacga aacctgcacc taaaacagta
    11941 attggcaata tgtcgtttaa agttgctaga atttatatat tagaacaagt tattgattat
    12001 ctaaatcaaa aatctacttt cgaaacaagt tttaaatatg gtaacgtgat taaagaatat
    12061 catgataaat tagcattttt aaaaactgta gagaaagatg atgaaaactc taaagaatta
    12121 gaacgtctac aaaaaattgc ttttaatgta gaaacaaaaa cattagagtc tttagtagat
    12181 gaaggacaaa taacgaatag tgtacttgaa aactatatgc gctatgctga aagaacacag
    12241 gtatatagac aagcatcatt aataagaaga atgattgtat tattacgagg tgctttatta
    12301 aaacgaagag tacaaacgag agtgaactcc gcatcttcac ttagtgttac ggataactta
    12361 atggaattaa ataaaattaa taaattagtc cattataatg tggttagtcg tttgtctaag
    12421 gaaacaacaa aagataatac acttgaagtt ggaatggttt gtgacggtta tttaatgcga
    12481 attgaaaact taacaccatc aaatttcttc aactcagcaa gtgaagatac gattactaaa
    12541 attaaattaa atgcattgag agaacaacgt cgcattttac gtgagttgat tgatacagat
    12601 gaagtatcag aaggtacagc gttaaaacta agagaagcca tcaattatga tgaaatggtt
    12661 attgtagata gtatgacgta gttcctaatt atgctaaaag ggattgatga aaaactgaag
    12721 ggcttttcat caatcccttt tattttaggg gaattgaata gatagtttta aactatacga
    12781 attattaata tttgagattt aattgaaata agttttaaaa attggaggag atagattaag
    12841 cgaagtcatt taaaggtgaa gttaagtgta ttcacaaaaa atagccacac tcatatgaca
    12901 tcggatgagt gtggcttaag gatctatggg gggaggaaac catagatgtt tactttgata
    12961 ggccagatta aatatcaaag tatgcgatta tttatagctt gatgcaaaag tggtatgcct
    13021 attaaaagtt actgcacata gcttttaata ttccgttcaa aggaaagggg catacaattg
    13081 aacaatctgt aatagtactt ttaaccagct atgctaaaag tctagtaggg agaacagttg
    13141 tccaatcaca taagaacctc taacttcgtt agtacgatta agaaaagctt tttagttagt
    13201 atgtaataca atttattgac gcgcgtgaat ctcttttata agagtgtgta gggaatggcg
    13261 ttgtataaat tgtattagaa gaacttctaa cgcatctctg tggttaaaag agatgaaggg
    13321 aacgacagtt taattaaaac tgcataagaa cttctagctt ttctctctcg ttcaaagaga
    13381 agcagctgtt cgcagtttaa tcaaaaccac ataaagcttt taactttact ctttgattta
    13441 aagagtgata aatgtttaca gtttaattaa aactgcataa gaacttctag cttttctctt
    13501 tcgttcaaag agaagcagct gttcgcagtt taatcaaaac cacataaagc ttttaacttt
    13561 actctttgat ttaaagagtg acaaatgttt acagtttaat taaaactgca taagaacttc
    13621 tagcttttct ctttcgttca aagagaagtt ctaataccac catatcgtgc gatcgggaac
    13681 ggtatatata ttaataggag ggtaatatat atttaacgca cgatatggga ctattagcct
    13741 tcgactttgt tatgttgatg tgtggcctaa aatattggag ataccaatat tttaggttgc
    13801 atcaacatca attcatctta cttcattaaa acacagcgtg ttatttcatg cttccgtgta
    13861 cagtttcaat atttgatttc atcattttgt agtaagagtc acctttagtg ccttctttac
    13921 cgattgaatc tgtgtacact tcaccaaaga tatctttctt cgtttcttca gataaacttt
    13981 ccattgcttt cttatcaaca cttgtttcta ctaataagtg ttttaatttg tgctttttaa
    14041 caaactcaat agcttgtctc atttgttcag gtgtaccttg tttttcagtg ttaatttccc
    14101 aaatataacc tggtgtaata ccgtattgtt ttgagaagta cttgaaggca ccttcacttg
    14161 taatcatggc acgttgttct tttggaatgt cattaaattt gtctttactg tcattattta
    14221 atttttccaa ttgagcaatg tatttgttac cttgcttttc ataatctgct ttatgttttt
    14281 tgtcgttatc gataaatgtt tgttgaattg tttttacgta tttaataccg ttatctaaac
    14341 ttaaccatgc gtgtggatct tgtttatctt tgttgccttc ttcaccgttt aaatagatag
    14401 gtttaacatc ttttgatact gcgataactt ttttatcttt taatgattta ccagcctgtt
    14461 ctaaggcttt ttcaaaccaa ccgttaccag tctctaaatt taatccgttg tataaaataa
    14521 cgtcagcgtc agttaacttt ttaatatctt taggtttaac ttcatattca tgaggatctt
    14581 gaccaacagg tacaatacta tgaatatcga cgttgtctcc accaacattt ttagccatat
    14641 catataaaat tgaattcgtc gttactactt ttaatttgcc atttgactta tcactgcttt
    14701 gtttaccacc agtaccacat gcagcaacta gaagtaataa ggctaataat aaaggtacta
    14761 attttttcat gttaaacttc ctcgtttctt tctattcgta aattttgtga aaaataatgt
    14821 gatgatataa attacaaacg tacaaagtac gattgtcgca ccactaggaa tgttgtaaat
    14881 atagctgtaa taaagtccga caattgaact tatgacactt attaaacttg ctataatcat
    14941 cattgagtat agttttttac taattaaaaa tgctgtagat gcaggtgtaa ttaataatgc
    15001 aactacaaga ataataccta ccgtttgaat acttgctact gttactaatg agagtaacaa
    15061 catcacaaag taatgtaata acgtcgtatt tagaccactc attctactaa acgttggatc
    15121 gaatgtagaa atcattaatg gacgatagaa aataatgatt agaataagga cgattgaacc
    15181 aatcacaata gttgttaaaa atgcactatt tgtgattgcc agtaaattac caaacagaat
    15241 atggtacaaa tctgtcgtag tgtttattaa gctaataata ataatccccg aagctaagaa
    15301 agcggtaaaa ctaattccaa tagcggcgtc aggtttcgtt ttactactag atgtgatata
    15361 accgataaaa atacttgcga tcataccagt tataagtgcg cctacaaaca ttggaatacc
    15421 aaataagaat gatagggcaa caccaggtaa tactgcgtga ctcattgcat ctcccattaa
    15481 tgaaagacca cgtaatacaa ttaaactacc aactgtacca caaactatcc ctacaataat
    15541 tgaagttatc aatgctcgat tcaagaattg atatgtaaat aaatgttcga caaactctaa
    15601 catgttatat tgctcctttg actagggtca ctacagtcag tgctactcat aaatgtttcg
    15661 tttaagcgag tgacactcat agcctcttca ctatcaccaa agtatcgtaa tgtttgattt
    15721 aatagaataa tgcgatcaaa gtattgcttt gcttttgata gatcatggtg gatgataaga
    15781 ataagttttc cttgttgttt taagttctcg atttttgtca tgattaattt ttcgctacta
    15841 aaatcaattc cgacaaacgg ctcatctaga aaataaactt cactttcgga catcaatgct
    15901 cttgctacta gcacacgttg taattgtcca ccacttaatt ctgaaatttg tcgatgacgt
    15961 aaagattcta attctaaatc gcttaataac tgtttgagtt tatcccttgc tgatttatta
    16021 ggtcgtctaa accatccaat ttctttgtag caacctgata aaatcacttg ttccacactt
    16081 ataggaaaat ctaaatcaat atgtgctttt tgtggaatat atgtaatatg ttgcagttgt
    16141 tgttgtatag gtttgttata taacaattta gtaccggtag cattaaattc accaattaaa
    16201 gacttgataa gggaagattt accagcacca ttcgggccca tgataccaat tatttcgccg
    16261 cgtactggta tcgataagga aatgttttta agtacatgct tattacctaa aaacagattt
    16321 aaatcttttg tttctaacaa acgtttatac ctcctaatta aaagtttagg ctaacctaat
    16381 taattgtata ataaactgag aatatttatc atgtcaagta aattcgtgat ataatataga
    16441 caatgtatgt gaggtgaaag tatgttaact gaagaaaaag aggactattt aaaggcaatc
    16501 cttacgaata atggcgataa aaactttgtg acaaataaaa tcttatctca atttttaaat
    16561 attaagcctc catctgtaag tgaaatggta ggacgtcttg aaaaagcagg ctatgttgaa
    16621 acaaaaccat acaaaggtgt tagattaaca gaggatggtt taacgcatac gcttgatatc
    16681 attaagagac atcgactatt agaattattt ttaatagaaa tattgaaata taattgggaa
    16741 gaagtacatc aagaagcaga aattttagaa catcgaattt cagatttatt tgttgaaagg
    16801 ctggatagcc tgttaaattt cccagaaact tgcccgcacg gcggtgtgat tcctagaaat
    16861 aatgaatata aagagaaata tataacaacg attttgaatt atgaacctgg tgatatcgtt
    16921 acaatcaaac gtgtgagaga taagaccgat ttgctaatat atttgtctag taaagatatt
    16981 tctattggta atgaagtgga aattgtatcg aaagatgaaa tgaataaagt aattatcatt
    17041 aaacgtaatg ataatgtaat tattgtcagt tacgaaaatg caatgaacat gtttgctgaa
    17101 aaataaaata aagaagccat aaagatatcc atgattgaac tgataaagac atatggataa
    17161 ttgctttagg cttctttttt attagttaat ttatcaagtg agtatatttg agtaaaatat
    17221 tcactgcata aagattgaag ataatccaga ttgtactata aatgaagata ggtacatgac
    17281 tgagttcttt aagtgcacta ccatcccact gtggactcgg acgctggaaa gtcaatttag
    17341 caatcgtcca actagattgt agaacttcgc ctaataatac acctaaaata tattgataac
    17401 tcattgtgac aagtagttga atttctacta tattttcatc ttttaatata aaatacaaca
    17461 tgatagaaat taaagttata acaacaatgg gtgagccttt tctagatgtt aaaattaaaa
    17521 aataaataaa tatcaataaa taggtaaata taaagaaact aggtatctga taatggctcg
    17581 acgctaaacc tatcaataac ataataggtg gcataaaata accaccaatc gttgtaagcc
    17641 attggcctgc tagatgtcta gattgtgtaa ttgcgaatcc ttgttgtaat gtctgttgtc
    17701 gctctcgtgg acttgttaca atgactaaat cttttgcacg gccaccagcg agtttattaa
    17761 acagtacatg accaaattca tgtgttaaaa cagggatata gtttaaaatg acatctaaat
    17821 agttcaaaac aggcttatgt ctatattgat gaatagcaat ataacaagct gcaacaataa
    17881 cgataatgta tatattaagt tgaattgtcg tattaaaaaa gtttgataaa taattcattg
    17941 ttaacctcat ataagatatt aatttaaagt ttgcttatca cttattataa atgatattgg
    18001 catcaatagc gttagacttt agacttacct tagttaaact aattttaatt tttgaaaagg
    18061 tgaatatgtg ttaaaataaa gcaaaatcat ttcgatataa ataggatgaa tataaatact
    18121 gttaatattg attacactaa cataataatg aaataagata ggagattcct gttatgactg
    18181 ttgaagaaag atccaataca gccaaagttg acattttagg ggtcgatttt gataatacaa
    18241 caatgttgca aatggttgaa aatattaaaa ccttttttgc aaatcaatca acgaataatc
    18301 tttttatagt aacagccaac cctgaaatag tgaattacgc gacgacacat caagcgtatt
    18361 tagagttaat aaatcaagcg agctatattg ttgctgatgg gacaggagta gtcaaagctt
    18421 cgcatcgttt aaagcaacct ctagcgcatc gtatacctgg tattgagttg atggatgaat
    18481 gtttgaaaat tgctcatgta aatcatcaaa aagtattttt gctaggggca actaatgaag
    18541 ttgtagaagc ggcacaatat gcattgcaac aaagatatcc aaacatatcg tttgcacatc
    18601 atcacggtta tattgattta gaagatgaga cagtagtgaa acgaattaaa ctgtttaaac
    18661 ctgattacat atttgtaggt atgggattcc ctaaacaaga agaatggatt atgacacatg
    18721 aaaaccaatt tgaatctaca gtgatgatgg gcgtaggtgg ttctcttgaa gtatttgctg
    18781 gggctaaaaa gagagcgcct tatatcttta gaaaattaaa cattgaatgg atatatagag
    18841 cattaataga ttggaaacgt attggtagat taaagagtat tccaatattt atgtataaaa
    18901 tagccaaagc aaaaagaaaa ataaaaaagg cgaaataatc atgatgacaa aaataaaacc
    18961 gaggaaatcc ttaaatggag attctcggtt ttttcggttt atttaataac gaagcgggac
    19021 tcatcgagtt tgtttctaaa ttctttttgt tcggctttgg atttcttttt aaaatcgtta
    19081 aggaaagctt catatttagg taatacatca tcaagttcac cgtaatcttt taactttccg
    19141 ccttcaatcc aagcaatctt agtacaaaat tgtctcactt gtcctaagtt atgactaacg
    19201 aaaaagatgg ttttgttttg ctctttaaac tcgtaaattt tatctaaaca tttttgtgca
    19261 aaagtttggt cacctacaga taaagcttcg tcaatgacta agatatctgg attaactgtg
    19321 atattaattg aaaaaccaag ttttgcacgc ataccacttg aatacttttt aactggttga
    19381 taaataaact caccaagttc actaaattca ataatcttag gtgtcatcgc tttaatttct
    19441 tttcgcttaa agcccataca taacatttta aattcgatat tttcaatccc tgtaagttgt
    19501 ccactcaagc cagcactaat tgcgataacg ctgacttcac cattacgatc cactttgcca
    19561 acagtaggcg acaaagaacc gccaatgata ttgctcaacg ttgatttgcc ggaaccattg
    19621 atgccaacaa gccctatgac gtcaccttca tatgctttta aactaatgtc atctaaagcg
    19681 aaaaatgttt tgtttttatg tttgggaatg agcgcatctt tcatacgttc tttatttgta
    19741 cgataaatac gatattcttt tgttacattt ttaatgttta ccgaaacgtt catttgtaga
    19801 ccttccttat tcacatttat ctagattata atatactact caacagttgt taaattttaa
    19861 aacctgttgt aaagtgtata gaagattttg ttattatcag agtgggtgtt ttgacacaaa
    19921 atgttaatca tcaatgataa caatgatatt taaaaactaa acttatttca acttacatga
    19981 ttgtatacta taatgtattt gtaataaact aatattttaa agaactagac aataattttg
    20041 atagcatcca tgtatagtga tagtatttac aacaattatt ataatactat ttagttaagt
    20101 agagaaatag ttaaacattt gaaagtgtgg tttaatggaa tgtcagcaat aggaacagtt
    20161 tttaaagaac atgtaaagaa cttttattta attcaaagac tggctcagtt tcaagttaaa
    20221 attatcaatc atagtaacta tttaggtgtg gcttgggaat taattaaccc tgttatgcaa
    20281 attatggttt actggatggt ttttggatta ggaataagaa gtaatgcacc aattcatggt
    20341 gtaccttttg tttattggtt attggttggt atcagtatgt ggttcttcat caaccaaggt
    20401 attttagaag gtactaaagc aattacacaa aagtttaatc aagtatcgaa aatgaacttc
    20461 ccgttatcga taataccgac atatattgtg acaagtagat tttatggaca tttaggctta
    20521 cttttacttg tgataattgc atgtatgttt actggtattt atccatcaat acatatcatt
    20581 caattattga tatatgtacc gttttgtttt ttcttaactg cctcggtgac gttattaaca
    20641 tcaacactcg gtgtgttagt tagagataca caaatgttaa tgcaagcaat attaagaata
    20701 ttattttact tttcaccaat tttgtggcta ccaaagaacc atggtatcag tggtttaatt
    20761 catgaaatga tgaaatataa tccagtttac tttattgctg aatcataccg tgcagcaatt
    20821 ttatatcacg aatggtattt catggatcat tggaaattaa tgttatacaa tttcggtatt
    20881 gttgccattt tctttgcaat tggtgcgtac ttacacatga aatatagaga tcaatttgca
    20941 gacttcttgt aatatattta tatgacgaaa ccccgctaac cattaataaa tggaagtggg
    21001 gttcattttt gtttataatt taagtaaata acatattaag ttggtgtatt atgaacgttt
    21061 taataaagaa attttatcat ttggtagttc gaatactttc taaaatgatt acgcctcaag
    21121 tgattgataa accgcatatc gtatttatga tgacttttcc agaagatatt aagcctatca
    21181 tcaaagcatt aaataattcg tcgtatcaga aaactgtttt aacaacacca aaacaagcgc
    21241 cttatttatc tgaacttagc gacgatgttg atgtgataga aatgactaat cgaacattgg
    21301 taaaacaaat taaggctttg aaaagcgcgc agatgattat tatcgataat tattacctat
    21361 tactaggtgg atataataag acttctaatc aacacattgt tcaaacgtgg catgcaagtg
    21421 gtgcattaaa aaactttggc ttaacagatc atcaagtcga tgtgtctgac aaggcaatgg
    21481 ttcagcagta ccgtaaagtt tatcaagcga cggattttta cttagtgggt tgtgaacaaa
    21541 tgtcacaatg ttttaaacag tctttaggtg caacagaaga gcaaatgctg tattttgggc
    21601 ttccgagaat taataaatat tacacagctg atagagcaac ggttaaggca gagttaaagg
    21661 ataaatatgg aattacaaat aagttggtat tatatgtacc aacatataga gaagataaag
    21721 cagataatag ggctattgat aaagcttatt ttgaaaaatg tttaccagga tatacactga
    21781 ttaataaatt acatccatca attgaagatt cagacattga tgacgtatct tcaatcgaca
    21841 cgtctacatt aatgctaatg tcagatataa ttattagcga ctatagttcg ctgccaatag
    21901 aagctagctt gttagatatt ccaactatat tttatgtgta tgatgaagga acatatgatc
    21961 aggtgagagg cctgaatcaa ttttacaaag caataccgga tagctacaaa gtgtatactg
    22021 aagaagattt aataatgacg atacaagaaa aagaacatct attaagcccg ttatttaaag
    22081 attggcataa gtataatact gataaaagtt tacatcagct cacagaatat atagataaga
    22141 tggtgacaaa atgaggttta cgataatcat acctacatgt aataatgagg caacaattcg
    22201 acaattgtta atatctattg agagtaaaga acactataga atcctttgta ttgatggtgg
    22261 ttctactgat caaacaattc ctatgattga acggttacaa agagaactca agcatatttc
    22321 attaatacaa ttacaaaatg cttcgatagc tacgtgtatt aataaaggtt tgatggatat
    22381 caaaatgaca gatccacatg atagtgacgc atttatggtc ataaaaccaa catcaatcgt
    22441 attgccaggt aaattagata ggttaactgc tgctttcaaa aataatgata atattgatat
    22501 ggtaataggg cagcgagctt acaattacca tggtgaatgg aaattgaaaa gtgctgatga
    22561 gtttattaaa gacaatcgaa tcgttacatt aacggaacaa ccagatttgt tatcaatgat
    22621 gtcttttgac ggaaagttat tcagtgctaa atttgctgaa ttacagtgtg acgaaacttt
    22681 agctaacaca tacaatcacg caatacatgt caaggcgatg caaaaagcta cggatataca
    22741 tttagtttca cagatgattg tcggagataa cgatatagat acacatgcta caagtaacga
    22801 agaagatttt aatagatata tcacagaaat tatgaaaata agacaacgag tcatggaaat
    22861 gttactatta cctgaacaaa ggctattata tagtgatatg gttgatcgta ttttattcaa
    22921 taattcatta aaatattata tgaacgaaca cccagcagta acgcacacga caattcaact
    22981 cgtaaaagac tatattatgt ctatgcagca ttctgattat gtatcgcaaa acatgtttga
    23041 cattataaat acagttgaat ttattggtga gaattgggat agagaaatat acgaattgtg
    23101 gcgacaaaca ttaattcaag tgggcattaa taggccgact tataaaaaat tcttgataca
    23161 acttaaaggg agaaagtttg cacatcgaac aaaatcaatg ttaaaacgat aacgtgtaca
    23221 ttgatgacca taaactgcaa tcctatgatg tgacaatatg aggaggataa cttaatgaaa
    23281 cgtgtaataa catatggcac atatgactta cttcactatg gtcatatcga attgcttcgt
    23341 cgtgcaagag agatgggcga ttatttaata gtagcattat caacagatga atttaatcaa
    23401 attaaacata aaaaatctta ttatgattat gaacaacgaa aaatgatgct tgaatcaata
    23461 cgctatgtcg atttagtcat tccagaaaag ggctggggac aaaaagaaga cgatgtcgaa
    23521 aaatttgatg tagatgtttt tgttatggga catgactggg aaggtgaatt cgacttctta
    23581 aaggataaat gtgaagtcat ttatttaaaa cgtacagaag gcatttcgac gactaaaatc
    23641 aaacaagaat tatatggtaa agatgctaaa taaattatat agaactatcg atactaaacg
    23701 ataaattaac ttaggttatt ataaaataaa tataaaacgg acaagtttcg cagctttata
    23761 atgtgcaact tgtccgtttt tagtatgttt tattttcttt ttctaaataa acgattgatt
    23821 atcatatgaa caataagtgc taatccagcg acaaggcatg taccaccaat gatagtgaat
    23881 aatggatgtt cttcccacat acttttagca acagtatttg ccttttgaat aattggctga
    23941 tgaacttcta cagttggagg tccataatct ttattaataa attctcttgg atagtccgcg
    24001 tgtactttac catcttcgac tacaagttta taatcttttt tactaaaatc acttggtaaa
    24061 acatcgtaaa gatcattttc aacataatat ttcttaccat ttatcctttg ctcaccttta
    24121 gacaatattt ttacatattt atactgatca aatgagcgtt ccattaatgc attccccatc
    24181 atattacgtt gcttctcgcc accaaggttt ttatagtctc ctgcacccat gataacttga
    24241 ttaattctaa atttacctcg tttggtagta atcgtatggt tgtaatttgc tgtatcactt
    24301 gatccagttt ttaaaccatc tgtacccggc aaactcattt ttgcaccttc caatgaaaag
    24361 ttgaatgtgt aatacgtaac tgcatgcgtt gttggtgcta actgctttgt aaagtctaat
    24421 attttaggtg tctctttaat cacgtgtaaa tctaaaatgg catagtctct agcagtcgtt
    24481 acagtacgtt cttggtcttt atactttgtt ggtgcaaatg tacgtaatct tgaattttca
    24541 gcacccgttg gattgacgaa atgtgtattt ttcattccga tagctttagc tttgttattc
    24601 attaaatcaa cgaaatcgct ggtgtttttt gaaaccttct tagctaaaat taatgccgcg
    24661 gcattactag aattagatac tgtaatttgt aataggtctg cgattgtcca tacttgtcca
    24721 ggatatagtt tcgtattact caactcaggt agtgtagaca taatatattc tttgttcgtc
    24781 attgtgactg tgtcatcaag tgaaagctgc cccttattta cagcttccaa tgttaagtac
    24841 attgtcatta atttagtcat agacgctgga ttccacttag tatcgatatt gtattgatac
    24901 agtaattgtc cagtttgact tacattaaca gcactcgtcg gttcgtatgc agccgacaaa
    24961 cctgcataac catattgatt tgctgcttgt acaggggtta cgtcactgtt agtagcttgt
    25021 gcatatggtg tcataatact taatgttaaa cataaaatga tgataataga tattaaattt
    25081 ttcataaagc gttaatcttc ccttttccaa ttcttaaata ttccctaaaa gcaatggtta
    25141 ttcctactta cggaaatcat tgctaattca cttcacctta attaaattgt tgaaaataaa
    25201 gttttctgca gttaatttga aaaataatgc aaatatatta cgtgtgtagc taaaggtgtt
    25261 ataatgtttg tacgaagagc aaacttactc aaaagcgatt aattttcatg ttttaatata
    25321 aagactttga gaagttatta caaaaaatgc aatagaaata ttctatcata taaatgttat
    25381 gagcggtatt ttggggcaac actttatttg atttttaaag ttttgttggg agaaagtata
    25441 tgatagaaat gcatgtatct atctaaatga attaactata aatttcaaac agaagaggta
    25501 aaactatgaa acgagaaaat ccattgtttt tcttatttaa aaaactatca tggccagtgg
    25561 gtcttatcgt tgcagctatc actatttcat cactagggag cttaagtgga ctattagtgc
    25621 cactgtttac tggacgaatt gtagataaat tttccgtgag ccatatcaat tggaatctaa
    25681 tcgcattatt tggtggtatc tttgtcatca atgctttatt aagcggatta ggtttatatt
    25741 tattaagtaa aattggtgaa aagattattt atgcgatacg ctcagtttta tgggagcata
    25801 tcatacaatt aaaaatgcca ttctttgaca aaaatgaaag tggtcaatta atgagtcgat
    25861 taactgacga tacgaaagtg ataaatgaat ttatttcaca aaagctacct aacttattac
    25921 catcaatcgt tacattagtt gggtcactaa tcatgttatt tattttagat tggaaaatga
    25981 cattattaac atttataacg ataccgatat tcgttttaat tatgattcct ctaggtcgta
    26041 ttatgcaaaa gatatcgaca agtacacaat ctgaaattgc aaacttcagt ggtttgttag
    26101 ggcgtgtcct aactgaaatg cgtcttgtta aaatatcaaa tacagagcgt cttgaattag
    26161 ataatgcaca taaaaatttg aatgaaatat ataaattagg tttaaaacag gctaaaattg
    26221 cggcagttgt acaaccaatt tcaggtatag ttatgttgct aacaattgca attattttag
    26281 gttttggtgc attagaaatt gcgactggtg caatcactgc aggtacatta attgcaatga
    26341 tattttatgt tattcagtta tctatgcctt taatcaatct ttccacgtta gttacagatt
    26401 ataaaaaggc agtcggtgca agtagtagaa tatacgaaat catgcaagaa cctattgaac
    26461 cgacagaagc tcttgaagat tctgaaaatg tattaattga tgacggtgta ttgtcatttg
    26521 aacatgtaga ctttaaatat gatgtgaaga aaatattaga tgatgtgtcg ttccaaatcc
    26581 cacaaggtca agtgagtgct tttgtaggcc cttctgggtc tggtaaaagt acgatattta
    26641 atctgataga acgtatgtat gaaattgagt caggtgatat taaatatggc cttgaaagtg
    26701 tctatgatat cccgttatct aagtggcgac gcaaaattgg atatgttatg caatcaaatt
    26761 cgatgatgag tggtacaatt agagacaata ttttatacgg aattaatcgt catgtttcag
    26821 atgaagaact tattaattat gctaaattag cgaactgtca tgattttatc atgcaatttg
    26881 atgaaggata tgacacgctt gtaggtgaac gaggattgaa actgtctggc ggacaacgtc
    26941 aacgtattga tattgctaga agttttgtta aaaatcctga tattttgtta cttgatgaag
    27001 caacagctaa tctcgatagt gaaagtgaat tgaaaattca agaagcttta gaaacattga
    27061 tggaaggtag aacaacgatt gtcattgcgc atcgtttgtc tacaattaaa aaagccggtc
    27121 aaattatatt cttagacaaa ggacaggtaa caggtaaagg tacgcattca gaactgatgg
    27181 catcacatgc gaagtataaa aactttgtag tgtctcaaaa attaacagat taattttata
    27241 tatataagta agcttggagc aaatacacat ataccatcga ggaaattaaa gtgtggcaca
    27301 ttgatggata tagatgttaa taaattgctt caagcttttg tctattttaa atcatttgag
    27361 aagttacgac ataataattc ttaaattaat gaaatcgata ttttaagaaa aaaatgctca
    27421 tggtataata caagttataa gcaaacatac atatattaaa tactgtagcc acgagtcata
    27481 attcttcgta ttttacatag caatttaact gattttagag tccacggtac agaagtttga
    27541 tatttcaatg tttctaaatt tttaaaaaat taaatcatag gtgggtgcca aatgttttta
    27601 ttaatcaaca ttattggtct aattgtattt cttggtattg cggtattatt ttcaagagat
    27661 cgcaaaaata tccaatggca atcaattggg atcttagttg ttttaaacct gtttttagca
    27721 tggttcttta tttattttga ttggggtcaa aaagcagtaa gaggagcagc caatggtatc
    27781 gcttgggtag ttcagtcagc gcatgctggt acaggttttg catttgcaag tttgacaaat
    27841 gttaaaatga tggatatggc tgttgcagcc ttattcccaa tattattaat agtgccatta
    27901 tttgatatct taatgtactt taatatttta ccgaaaatta ttggaggtat tggttggtta
    27961 ctagctaaag taacaagaca acctaaattc gagtcattct ttgggataga aatgatgttc
    28021 ttaggaaata ctgaagcatt agccgtatca agtgagcaac taaaacgtat gaatgaaatg
    28081 cgtgtattaa caatcgcaat gatgtcaatg agctctgtat cgggagctat tgtaggtgcg
    28141 tatgtacaaa tggtaccagg agaactggta ctaacggcaa ttccactaaa tatcgttaac
    28201 gcgattattg tgtcatgctt gttgaatcca gtaagtgttg aagagaaaga agatattatt
    28261 tacagtctta aaaacaatga agttgaacgt caaccattct tctcattcct tggagattct
    28321 gtattagcag caggtaaatt agtattaatc atcatcgcat ttgttattag ttttgtagcg
    28381 ttagctgatc tatttgatcg ttttatcaat ttgattacag gattgatagc aggatggata
    28441 ggcataaaag gtagtttcgg tttaaaccaa attttaggtg tgtttatgta tccatttgcg
    28501 ctattactcg gtttacctta tgatgaagcg tggttggtag cacaacaaat ggctaagaaa
    28561 attgttacaa atgaatttgt tgttatgggt gaaatttcta aagatattgc atcttataca
    28621 ccacaccatc gtgcggttat tacaacattc ttaatttcat ttgcaaactt ctcaacgatt
    28681 ggtatgatta tcggtacatt gaaaggcatt gttgataaaa agacatcaga ctttgtatct
    28741 aaatatgtac ctatgatgct attatcaggt atcctagttt cattattaac agcagctttc
    28801 gttggtttat ttgcatggta atatgtcgaa gagtgactat gataatacat tttaactaat
    28861 aaatatgtcc aggcatgtcg tctattgata taggtgagat gcttggact
//
LOCUS       DANJFZ010000026        27698 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.026, whole genome shotgun sequence.
ACCESSION   DANJFZ010000026 DANJFZ010000000
VERSION     DANJFZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.026"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            14..128
                     /gene="rrf"
                     /locus_tag="PWN45_000963"
     rRNA            14..128
                     /gene="rrf"
                     /locus_tag="PWN45_000963"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(338..1606)
                     /gene="ilvA"
                     /locus_tag="PWN45_000964"
     CDS             complement(338..1606)
                     /gene="ilvA"
                     /locus_tag="PWN45_000964"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDL0622745.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(1621..2193)
                     /gene="leuD"
                     /locus_tag="PWN45_000965"
     CDS             complement(1621..2193)
                     /gene="leuD"
                     /locus_tag="PWN45_000965"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL0622746.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            complement(2194..3564)
                     /gene="leuC"
                     /locus_tag="PWN45_000966"
     CDS             complement(2194..3564)
                     /gene="leuC"
                     /locus_tag="PWN45_000966"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL0622747.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            complement(3578..4624)
                     /gene="leuB"
                     /locus_tag="PWN45_000967"
     CDS             complement(3578..4624)
                     /gene="leuB"
                     /locus_tag="PWN45_000967"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL0622748.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            complement(4627..6156)
                     /locus_tag="PWN45_000968"
     CDS             complement(4627..6156)
                     /locus_tag="PWN45_000968"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL0622749.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            complement(6186..7190)
                     /gene="ilvC"
                     /locus_tag="PWN45_000969"
     CDS             complement(6186..7190)
                     /gene="ilvC"
                     /locus_tag="PWN45_000969"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL0622750.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            complement(7327..7581)
                     /locus_tag="PWN45_000970"
     CDS             complement(7327..7581)
                     /locus_tag="PWN45_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDL0622751.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            complement(7581..9350)
                     /gene="ilvB"
                     /locus_tag="PWN45_000971"
     CDS             complement(7581..9350)
                     /gene="ilvB"
                     /locus_tag="PWN45_000971"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDL0622752.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            complement(9378..11066)
                     /gene="ilvD"
                     /locus_tag="PWN45_000972"
     CDS             complement(9378..11066)
                     /gene="ilvD"
                     /locus_tag="PWN45_000972"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL0622753.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            complement(11384..11539)
                     /locus_tag="PWN45_000973"
     CDS             complement(11384..11539)
                     /locus_tag="PWN45_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622754.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            11577..12038
                     /gene="tsaE"
                     /locus_tag="PWN45_000974"
     CDS             11577..12038
                     /gene="tsaE"
                     /locus_tag="PWN45_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL0622755.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            12019..12681
                     /gene="tsaB"
                     /locus_tag="PWN45_000975"
     CDS             12019..12681
                     /gene="tsaB"
                     /locus_tag="PWN45_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL0622756.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            12654..13118
                     /gene="rimI"
                     /locus_tag="PWN45_000976"
     CDS             12654..13118
                     /gene="rimI"
                     /locus_tag="PWN45_000976"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0622757.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            13111..14136
                     /gene="tsaD"
                     /locus_tag="PWN45_000977"
     CDS             13111..14136
                     /gene="tsaD"
                     /locus_tag="PWN45_000977"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL0622758.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(14238..15848)
                     /locus_tag="PWN45_000978"
     CDS             complement(14238..15848)
                     /locus_tag="PWN45_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDL0622759.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            15937..16065
                     /locus_tag="PWN45_000979"
     CDS             15937..16065
                     /locus_tag="PWN45_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622760.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(16209..18137)
                     /gene="abc-f"
                     /locus_tag="PWN45_000980"
     CDS             complement(16209..18137)
                     /gene="abc-f"
                     /locus_tag="PWN45_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDL0622761.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            18390..19025
                     /locus_tag="PWN45_000981"
     CDS             18390..19025
                     /locus_tag="PWN45_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0622762.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            19329..20408
                     /locus_tag="PWN45_000982"
     CDS             19329..20408
                     /locus_tag="PWN45_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL0622763.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            20468..20692
                     /locus_tag="PWN45_000983"
     CDS             20468..20692
                     /locus_tag="PWN45_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL0622764.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            20900..22150
                     /locus_tag="PWN45_000984"
     CDS             20900..22150
                     /locus_tag="PWN45_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL0622765.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            22333..23283
                     /locus_tag="PWN45_000985"
     CDS             22333..23283
                     /locus_tag="PWN45_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0622766.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            23432..24916
                     /locus_tag="PWN45_000986"
     CDS             23432..24916
                     /locus_tag="PWN45_000986"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL0622767.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            24913..25873
                     /locus_tag="PWN45_000987"
                     /pseudo
     CDS             24913..25873
                     /locus_tag="PWN45_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
     gene            complement(26061..26168)
                     /locus_tag="PWN45_000988"
     CDS             complement(26061..26168)
                     /locus_tag="PWN45_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622768.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(26240..26956)
                     /locus_tag="PWN45_000989"
     CDS             complement(26240..26956)
                     /locus_tag="PWN45_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0622769.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(26975..>27698)
                     /locus_tag="PWN45_000990"
     CDS             complement(26975..>27698)
                     /locus_tag="PWN45_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0622770.1"
                     /translation="IFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEM
                     RKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVRE
                     IKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDP
                     IIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNAD
                     NVLLDTIIENGFFIQKVEIINN"
BASE COUNT         8988 a         4512 c         4817 g         9381 t
ORIGIN      
        1 ttacattcat atgtctggtg actatagcaa ggaggtcaca cctgttccca tgccgaacac
       61 agaagttaag ctccttagcg tcgatggtag tcgaacttac gttccgctag agtagaacgt
      121 tgccaggcaa aaagtggatg cgatgagccg cattgagacc gcaaggtctc tttttttatg
      181 tctaaaacgt caaaataaaa gcaaacacaa agaaagatgg cttggcgaag tgaaaacgtt
      241 tgaatctgac gaaacgagaa atgtaaagta taataaaaag cagtcataag atgatttcaa
      301 ttagaaatca atttatgact gtttttctta ctatgtgtta aattaacaat gaatataaca
      361 tcttattttc attaatataa atattggaag gatcgaaatg atttacacgt tgtttgagtt
      421 gtattaaatc atcatgatct ttaagttgaa taccaataat gacagtacct gtattttgag
      481 aagatttttt taagtattca aatttagtaa tatcgtcttg aggtcctaat acgtcattta
      541 caaattctct caatgcacct ggacgttgag ggaaatttaa gataaagtaa tgcttcattt
      601 cttcgtatag taatgaacgt tcttcaattt ctttcattcg attaatatca ttattaccac
      661 cactaatgac acaaaccact gttttacctt taatatgatc tttatagttt tcaagcgcac
      721 ttacacttaa tgcgccagca ggttctgcta caattgcttg ttttgaatac atatctaaaa
      781 tcgtagaaca aactgcacct tcatctactt gaacgtaatc atctacattt tcttttgcaa
      841 tttcaaatgt aatatcgcca actctagcta cagatgcacc gtccacaaat ttatcgatat
      901 taggcaatgt gactacctga ttatttacca caacagattc atacatacta cttgcacctg
      961 aaggttcaac acctataatt ttcgtggtag gtgaataggt tttaaagtaa gtactaatac
     1021 ctgaaattaa accgccacca ccaattgcgg caaatagata atcaaagtta acattgtcag
     1081 actttgcttg ttctagcatt tctttagcaa gcgtaccttg tccagaaatt gtatgaacat
     1141 tattgaatgg atctataaag ttcatttgat gttcacttgt ataagttaaa gcttcagcta
     1201 aacagtgatc aaatgtatca ccagtgagta caacttcaac gttactattt ccaaagaact
     1261 ttacttgatt taccttttgt aaaggtgtag tgactggcat aaagataaca gcgtttaaat
     1321 taagtttttt agctgtatag gcaacacctt gagcatgatt acctgcactt gcacatgtaa
     1381 tacctttact tttagcttca tctgataaaa cagaaatagc gttgtaagca cctcttaatt
     1441 taaaagaacg tacccattgt aaatcttctc gttttaaata gactttacaa tcatactttt
     1501 gagataagta atggtctaat tgtaaaggtg tttctttgac aatatcttta agtcttaaaa
     1561 atgcctcatc gatatctttc gtagaaactg ttgttttgac tgtcataata ttcaactccc
     1621 ttaaagtgat ttttcatatt tttctattaa tgattcatat tgtagggtga ttgcaatgtc
     1681 atctaagcca tttacaagtt tattcttcca agtttcatca atttcaaaat ggaaacgctt
     1741 gtctggtgat gacacagttt gatttggtaa atcgacctca atttcaacat attgtgcaag
     1801 atgttcacgg gcactttttt ctaaaacgat aggcaacatc gcatttttag tgcaattcat
     1861 ataaaatatg tcactgaaac ttcctgcaat aataatatga aaaccatagt ccttaagagc
     1921 ccaagcagca tgttcacgac ttgaaccaca tccaaaatta tctccagtaa ttaaaataga
     1981 agccccttta tattgtggtt tgttaggatt gaaatcagga ttatctgaac catcaggtaa
     2041 gtaccgccat tcatcaaaag caaatggacc aaagccactt tttgaaatac gctttaagtg
     2101 taccttagga atgatttggt ctgtatcgat attgtcgttg aagagaggga ctattttacc
     2161 tttatatgtt gtaataggtt tgattgctgc catttaaaca accacctttc ttacgtccac
     2221 aaatttacca tgaatagctg ctgctgctgc catagcaggg gatactaaat gtgttcttgc
     2281 acctttgcct tgtcgtcctt caaagtttcg attacttgta gatgcacaat gtacgccctc
     2341 aggtacttgg tcaggattca tgcctaaaca cattgaacat cctggttcac gccattcaaa
     2401 tcctgcattt ttaaagatag tatctagacc taatttttct gcttcttttt ttactgtacg
     2461 agaacctggt acgacaatag ctgtaatatt tggatgaact ttatttcctt taacaatatg
     2521 actagcttca atcaaatctg atagtctagc atttgtacat gaaccgagaa aaacataccc
     2581 taagtcgatg tcttcagctt tttgacctgg ttctaacccc atataatcat acgcacgttg
     2641 atcgttgata tcattgattt cagggaatgg ttcactgaaa ttaacaccca tttcaggatt
     2701 agttccccat gtcacttgtg gttctaatgt tgaaacatca agttcaatta cacgatcaaa
     2761 tatcgcgtcg tcatcagaat atagctcacg ccacttatca actgatttag cgaagttatc
     2821 ggcaaatggt ctccctttaa catattcaaa tgttatatca tcaggttgga ttatgccgta
     2881 tttggcacca ccttcgatag ccatgttaca aatagtcatt cgaccatcca ttgaaaggtt
     2941 tttaattgtt tcgccagtaa attccaaagc atagcctgta ccaaagtcaa caccatacgt
     3001 tttaattaaa tgcagaataa tgtccttagc atagacgcct gttggtaagg taccattaat
     3061 atcgattttt aagtttttgg gttttgtttg ccatagcgtt tgagttgcga aaacatgttc
     3121 aacttcactt gttccaattc caaatgcaat agcaccaaat gctccatgtg ttgctgtgtg
     3181 agagtcacca caaacgattg tcttgccagg ctgtgtaagt cctgtctcag gtcctaccat
     3241 gtgaacaata ccttgttcat cagaacccat atcaaaaata tgcaccccaa aatctatggc
     3301 gtttttttgt aatgttgtga tttgtttgtt tgcaatttca tctttaatat tgaatatatc
     3361 aatagtagga acattgtgat cgagtgttgc aaatgttaaa tctgggcgtc ttaattttct
     3421 gttttgaagc ctaagtcctt caaatgcttg aggagaagta acttcatgta taaggtgtaa
     3481 atcaatgtat aatagttgcg gttcgcccaa tttcccgtat aacacatgtc tgttccacac
     3541 cttgtcaaat aatgtttgac ccatttacat ctccccctta gtgattcaat ttttgagata
     3601 gaatttcgaa aatatcagta gtattcaatt tgccgcctaa atctgctgtc gtttgcccat
     3661 gttcaatcat gctataaata tgttgttcta attcatctgc agcatctggt tgatttaagc
     3721 tttcacgtaa acacatcgct aaagatagaa tcattccaaa tggattggca acgtttttac
     3781 ctgcaatatc tggtgctgat ccatgaatag gctcatacaa tcttggacca tcgttactaa
     3841 aactagcaga aggtgataaa ccaagtgaac caggaatcac tgaagcttca tcacttaaaa
     3901 tatcgccaaa taagttttca catacgatga cgtcaaattg ttttggattt gtgattaaat
     3961 gcatactaca agcatcaaca agtaagtgat ttactgttac ttctggatat aattgactta
     4021 cttcatttac gactttgcgc cacaatttac tagaagctaa tacattttct ttatcaactg
     4081 atgttagttt tcctcgtctt gaagcggcca atttaaatgc tacgtgaaca atgcgttcta
     4141 tttcttctct tgtataagta agagaatcta aggcctcgtg attattaaaa tgtctaggtt
     4201 ctccaaaata aataccactt gtcaattcac ggactataac taaatctgtg ccttcaacgc
     4261 gttcttcctt taaaggtgat aaagaactag cgcctttgac aacggtagtg gggcgtatat
     4321 ttacaaataa atttaaggat ttacgcaatt ttaataatcc ttgttctggt cgattgttag
     4381 gatctgtcca tttaggtcca ccgattgcac ccagtaaaat agcatctgct cttttacacg
     4441 catttaaggt tttctcagtt aaaggctcgc cgaatgtatc aatagaggca ccaccaaatt
     4501 cgtggtgctc tatttgataa ttaaagttat atttattact tataatttca agcaatgata
     4561 gagatccgtt caaaatttct ggaccgattc catcaccagg tagggcaaca atgttataag
     4621 tcataattaa ttacctacct tctcaacatt ttcagctgca aatttagcat gtgcttctac
     4681 gtatgcttta caagaggctt gtaaaatatc atgatcaata ccaaagccat tgacagtctt
     4741 accttcaatc aataaattta catgtacttc tgcttgggca tcagtacctt cagtgacaga
     4801 attaatacga taatcaatta attctgtttc tttctggaaa atacgatcaa ctgcattgta
     4861 aattgctacg attgaaccag taccaatact tgaatcctgg taaatatgac cctctttatc
     4921 tttaacaaca acaacagcac tttgaaggcc gctagagaca tattgtagtt gcagtgtttc
     4981 caatttataa agtgcttgat gctcatgctc agaaccttga ataatcgcat gaatatctct
     5041 atctgaaaca gatttctttt tgtccgcaat ggccttgaat tgtttaaata aatctatttg
     5101 cgcttcttta tcaatgtcat aacctaatgc ttttaacttc tctgagaagg cgtgtttacc
     5161 agataatttt cctaatggaa gttcagtcgt gcttacacca acaagttgag gtgtcataat
     5221 ttcatatgtt tcacgatgtt ttaatacgcc atcttggtga atacctgatt catgactaaa
     5281 tgcattttgg ccaacaattg ctttatttct aggcactcga atacctgcat atcttgaaat
     5341 taaatccgat gtttttttag tttcttcgag attaagagca gtttgagcac cataatgatc
     5401 atttcgaacg tatagtgcaa gcgcgacttc ttcaagtgct gcattacctg ctcgttcacc
     5461 aataccattt acagtgcctt caattcgtct cgcaccgcct tcaattgcag ctaaactatt
     5521 tgaaacagcc attcctaaat cgtcatggca atgagcacta taaataattt catttgaaga
     5581 tgttacagat tctgttaagg ttttgaaaat atgtgcatat tcatcatggt aactgtagcc
     5641 gactgtatca ggaatattaa taactgtagc tccagcgtca acggcagttt gtacacattt
     5701 cactaagaat ggtaattcag tacgcgttgc atcttcaggt gaaaattgaa caacgtcaaa
     5761 taattgtttc gcgtatgtga catgttcttt aatagatgct aaaacgtctt cttgagacat
     5821 tttaagttta tgttcaagat gaataggtga tgttgctata aaaacatgca cgaccggctt
     5881 cgctgcatct tttgttgctt catatacagc atcgatgtca gattttttac atctagctaa
     5941 accacataca gccgttgttg ttaatgtttg tgcaattgct tgaacagatt taaagctacc
     6001 tgtacttgaa gcaggaaatc cagcttcaat aacatctaca ccccattttt ctaattgcaa
     6061 tgcaatacgc aagcgttcat caaaagtaaa attcactcct ggtgtttgtt caccgtctct
     6121 tagtgtcgta tcaaaaattt gaatatgact actcatattt aacaactcct cattgtaggt
     6181 ctatcttatt tttcaatgct tttagattta ataaaaggca tcatttcgcg taattcacga
     6241 ccaacttttt caatttgatg accatgttgt tcttcgcgta atttataaaa ttctttgaat
     6301 ccatttttat tgtcttcgat aaagcgatta ctgaagttac cattttggat atcagttaat
     6361 acagctttca tattttcttt aacatctggt gtgataacac gtggtcctga aacatagtca
     6421 ccaaattcag cagtatttga aattgagtaa cgtacatttt ccataccgcc ttcatacatc
     6481 aaatcaacga ttaatttcat ttcatgtaat acttcaaaat aagctaattc tggttgataa
     6541 cccgcttcta ctaatgtttc aaagccactt tgaattaatt tcgatacacc accgcaaagt
     6601 actgcttgtt caccaaataa atctgtctca gtttcttctt taaatgttgt ttcaataaca
     6661 cctgcacgag ttgcaccaat accttttgca taacttaaag caatattacg tgcttgacct
     6721 gaagcgcctt gttgaatacc aaatagtgat ggtacagcag aaccttcaac aaatgtacgt
     6781 ctaactaaat gacccggtcc tttaggagct actaaaaata catcaacatc agctggtggt
     6841 tgaataacac caaaatgaat gttaaagcca tgagcaaatg caagcgcatt atgtttttct
     6901 aaatttggtt caatttcgtt tttgtataca tcaccttgaa tttcatcagg taatagcacc
     6961 ataattacat cagcttgctt aactgcttct gcaacaggga acacatcaaa tccatcttct
     7021 ttagctttgt caaaagaacg acctgggcga atgccgatga ctacatcata tccattgtct
     7081 tttaagtttt gtgcatgcgc gtgaccttgt gatccataac ctactactgc aatttttttg
     7141 ccttgtaaag cgtccgtttt tacatcttga tcataataaa ctgttgtcat aataaattcc
     7201 tccaaataat taaatatttt gtgtttcgaa aattctgaaa tataactttg taatagtggc
     7261 agatgttaac tagcagccta tgatatatct ataactaatt agcgcatcat tgcctttgtc
     7321 ttaaaattaa gcttcgttat caacaaggtc gtagcattca accgttaaaa cattaatttg
     7381 ttgttttaat tttttaatta atatatgaag tgatgtatca tcttgaatat cgacttgaat
     7441 ttccatgtta gaaatcccag gttgttcaga atgtgtaaca tgtaatgtat cgatattata
     7501 ttgtaggcga acaaaagcac ttgtaattcg atttagcgtg ctgacttgat ccgcaacttg
     7561 taatttaaga attcttgtca ttataagccc tccatttcat gatttgattt gccactcgga
     7621 accattgggg ttacagcttc agtaggggaa atacgaacct caattaaagc tggtccttga
     7681 taagcaaacg ctgcatctaa ttgttcttcc agttgttctg gcttatcgat taagaaacct
     7741 ttgacgccat atgcttctgc cattttcata aaatcaggtt gaccattaaa tactgagtgt
     7801 gagaagcgtt gattaaagaa cttatcttgc cattgtttaa ccatacctaa tgttccatta
     7861 ttgattagta cgattttgac atctaaacca tattcgggta aaagtgccat ttcttggttt
     7921 gtcatttgga aaccaccgtc accgacgaaa catacgactg ttttatcagg attagctaat
     7981 ttggcaccaa ttgacgaagg aataccgaat cccattgttc ctaaaccacc gcttgtaacc
     8041 cattgtccgt gatttttaaa tggataaaat tgagctgccc acatttgatg ttgtcccacg
     8101 tctgtagtaa caattgcttc accatttgta attttgccga tatattcgat tgtttgttgt
     8161 ggcttacaaa atacttgatc ttcttcacca agtttaaatg ggtgtttctg cttattattt
     8221 tgacaatgtt taacccagtc actgtgttct atagtctcaa catttttatc atttaaacat
     8281 tctaaaaatc ttttacagtc tgcaataata cctaaatcta catgaataac tttattgatt
     8341 tctgaaggat caatatctac atgtacaatt ttggcgttag gtgcaaaggc atcaggtttg
     8401 cttgctaatc tatcatcgaa gcggctacct aaattaatga gtaaatcaca ctcagttaat
     8461 gccatgttac tagcataaga accatgcatt cctcccatac ctaaaaatag tgtatcctca
     8521 taaggtacag cacctagacc tagtaaagta gtaactgttg ggatttgatg tttattaaca
     8581 aactgtgtta ataattgatt tgattttgat tgattaatac ctgcgccggc taatacgaca
     8641 ggctttttcg cttcttttaa aagtgagatg aaagtgtcaa tgtctttatt ttctggttct
     8701 gtaacaactt cataacctgg aatattgatt tcgtcgcata aatccacatt tgtagctaaa
     8761 acacccatat cttttggaaa atcaatcact acaggacctt tgcgtccaga attagctaca
     8821 tggaaagctt cgtgtacgat tttagggata tcttcaacac gtttcacttg ataattttgt
     8881 tttgtaattg gtgaagtcat agatagaata tccgcttctt ggaatgcatc tttaccaatg
     8941 cctggtgtag caacttgtcc agtgaataca actagaggta aagagtcgca atgtgcatcc
     9001 gtaatacctg tcattacatt agttgcacct ggaccgcttg taactacaac gacgccagtt
     9061 ttaccagata cacgtgcata accttctgca gcatgaacag caccttgttc gtgtcttgct
     9121 aaaatatgtt tgattttacc atcataaaac gtgtcatata aaggtagtac ggcaccacca
     9181 ggataaccga ataaataatc cacattttct ttaagtagag cttctactag cacttctgat
     9241 cctgaacgca tatcatttaa agttttttca tttagttgtt caggttcaag tgattcagcc
     9301 atttttaaag ggtcaatatt ttggtttact tcatgttgag ttttagacat tttaaccatc
     9361 tccaatataa aaataaatta aattaaattc tcagggactt gcatgacgcc acctgtattt
     9421 gcgctagtta ctagggcagt ataacgagct agataacctg tttttacttt cgctttaaat
     9481 ggtgttaaag attctcggcg acgcgctaga acatcttcag gctggtttac gtttaatgta
     9541 cgatttgtta aatcaatagt aatctcatca ccatcttcaa ttaaggcaat tggtccacca
     9601 gatgcagctt caggggaaat atgaccaact gcaatacctc ttgtggcacc ggaaaaacgc
     9661 ccatcagtaa ttaatgcaac atctttacct aagccgcgac caacaataga ggaagtaggt
     9721 gctaacattt caggcatacc tggtccacct ttaggtcctt catatctaat gacaacgacg
     9781 tggcctgcac gaacggtacg attgtctatt gcttcaacag cttcatcatg cgaattgaaa
     9841 caaattgctt tcccagtaaa tgttttgata gatggatcaa cgccgccaac tttaataact
     9901 gcgcctttag gggcgatatt accaaataag atagataaac cgccttgtgc atcatatggt
     9961 gcatcaagag ggtgaatgac atcaaagttc ttaatttctt tgccttcgtt attttcacgt
    10021 aacgttttgc cagtaactgt gattctatct gggtgtaacg tgccatcttt cttcatcaat
    10081 tcattaataa ttgctgggac gccaccagct tcatgcacat catgcattga atacgatgaa
    10141 ctaggtgcta tttttgataa atatggcgtg cgtttggcaa tagcattaat gcgctctaag
    10201 tcataatcaa taccagcttc attggcaatg gctaacgtat gcagtaccgt gtttgttgaa
    10261 ccacccatag ccatatcaag tgcaaatgca tcatcaattg cttcgcgagt aacgatatca
    10321 cgtggtttta aatcattttt aatattttca actaatttaa aagctgcttg gcgaatcatt
    10381 tctcgtcgct gatcactgac tgcaagtgca gtaccgttgt atggtaatgt tagacctaaa
    10441 acttccatca aacagttcat tgaatttgca gtaaacatcc cagcacatga accacaagta
    10501 gggcaggcat tttgttccat atctaaaaat tcttctttag aaatcgatcc ttctttaaat
    10561 gcgccgactg cttcaaacat tgatgaaagt gttaatgctt ttccatgtgc agataagcca
    10621 gctttcattg gtccaccaga gcaaaagata gctggtacgt ttgtcctcat ggctgctaaa
    10681 atcataccgg gtgtaatctt gtcacaatta ggaatgtaaa atacgccgtc aaaccaatga
    10741 gcgttaatta cagtttcagc tgcatctgca ataatttcac gtgatggtag agaatatcgc
    10801 attccgatat gtcccatagc tattccatca tcaacaccaa ttgtattgaa ttcaaatgga
    10861 atggcaccgg cttctctaat tgcttcttta gctatatctg caagctctct caagtgaaca
    10921 tgtccaggaa caatatcaat ataagagtta caaatagcta caaatggttt gttcatatca
    10981 gttggacttt ttagcgcgcc cgtggcatgt aaaagacttc ttgctggtgc ttggtgatct
    11041 ccttttttga tcatgtcgct tcgcatagta aattcccccg taaattttaa tgattaatca
    11101 tgttttatag taataaaaat aagtaattga aagataaaac ataaaaaaac gtcccaatat
    11161 tattgggacg tttacaagtc ccattcaaac agaaatagga cttaaagcgt ttagtaaata
    11221 tttactaacg ttcgagtcct aaagagtata ataaaataat aatatttgaa gttgtcggtt
    11281 gatgttcagt atattgatta agcattttat tatactcctt ccgtgaatgg tatatatcac
    11341 tttaatagtt agaattgtct aaaaatttga taaatttaat ttactatatc tgctttagta
    11401 tgtcaacaaa tattttaaat tttcttacaa ttaactttga atagataatc tgtttcaaag
    11461 ataagaaatt taagttgaaa tttgttacaa tataaaggtt aaatataaag ttagatacga
    11521 ttgaacaatg gataatcatt tttatgaatg aaaagcataa tataggagaa tcaacattga
    11581 taaagataaa taatttagat gaaatgaatc aatttgctat atttttagtt gagcaattga
    11641 aaagtggtga tttgatttta cttaacggag atttaggagc aggtaaaaca acgttaacgc
    11701 aatttatagg aaaagctctt ggtgtaagac gtacgattaa ttccccgaca tttaacatca
    11761 ttaaatcata taggggtaaa aatttaaaat tgcatcatat ggattgttat cgcttagaag
    11821 attctgatga agatttagga tttgatgaat ttttcgaaga tcaggcaatt actgttattg
    11881 aatggagtca atttataaaa gatttacttc cagcgacgca tttatctatt aacatttcaa
    11941 caatatctga aaatacaaga caaattgagt tgttcgcgca aggagaacat tatgaacaaa
    12001 ttaaggaggc aattatccat gaattcgctg ctcattgata catcgaatca accattatca
    12061 gtagcattga tgcaaaacga tgaagtttta gctgaaataa ctacggattc taaacaaaat
    12121 cattcagtgc aattaatgcc tgcgattagt cagctatttg agcagagcaa aatagctaaa
    12181 cagcaattag atgcgattat tgttgctgaa ggtcctggtt catatactgg cttacgtatc
    12241 ggtgttactg ttgctaaaac attagcgtat gcgttagatg ttaaattata tggtgtttca
    12301 tcattaaagg cattggcagc aacaatcgat catactgata aattattggt tcctgtgttt
    12361 gatgcaagac gacaagcagt ttatacaggc atattccagt ggcaaaatgg tcaattagtg
    12421 actattttag aagatcaata tatgtcgata gaggcattgc gagctttttt agaagattta
    12481 aaccaaccat ttatttatat tgggaaagat actgtaaaat tacaagatga attacaaggt
    12541 gaagtgattg cgcagctacc aaatgcatca gtcatgtatc aattgctaga taaaccttca
    12601 gacgtacata catttactcc taaataccat aaattagctg aggcggaacg aaattggatc
    12661 aacagtcaaa agaacaatta aacattagag agatgactaa agaagatgtg ccacaagtct
    12721 ttgatcttga gcgtcggagt ttcaatgaca gttcttggac aattgacgca ttttatcatg
    12781 aaatagagca aaataacttt gcgaaatatt ttgtcttaga atttgaacaa caaattattg
    12841 gttatttagg tttatggatt gttatcgatc aagctcaaat tacaacagtt gcaatcgatg
    12901 ataattatag aggctatggt ttaggtcaaa tgcttttaaa atacggaata aactatgcaa
    12961 gtcacacttg tgatgtgatg agtttagaag taagagttaa taataaagtg gcacaacatg
    13021 tatatgaaaa tttaggattt caatatggtg gtaaacgtaa aaattattat ggtgaaggtg
    13081 aggacgcaat ggttatgtgg gtgaatttaa atgactaaag atatattaat actagctgtt
    13141 gaaacaagtt gtgatgaaac aagcgttagt gttataaaaa atggcagaga tattttatca
    13201 aatacagttt taagtcagat tgaaagtcat aaacgatttg gcggtgtcgt tcccgaagtg
    13261 gcaagtagac atcacgttga aggtataaca gcaacaataa acgaggctct aggggatgcc
    13321 gatgtatcaa tagaagatat tgatgccata gcggttacag aaggccctgg actaattggt
    13381 gcgttactaa taggtgttaa tgcagccaaa gcattggcat ttgcttacga taagccactt
    13441 attcctgttc atcatattgc aggacatata tatgctaatc acatagaaga gccattaaca
    13501 ttcccgctaa ttgcacttat tgtttcaggt ggacatactg aattagttta tatgaaggat
    13561 catttatcat ttgaagtcat tggtgaaaca cgagatgacg cagtaggtga ggcttatgat
    13621 aaagtggcac gaacaattgg tttaaattat ccaggtggtc cacaagttga tcggttggct
    13681 gctgaaggtg aagatactta ttcattccct cgtgtttggt tggataaaga tagttatgat
    13741 tttagtttta gtgggttgaa aagtgccgta atcaatcaac ttcacaatca acgacaaaaa
    13801 aatattccaa tcattgaagc taacgtagca acgagctttc aaaacagtgt tgtagaggtg
    13861 ctcacgttta aagctattca agcttgtaaa gaatatggtg ttcagcgatt aattgttgct
    13921 ggtggcgtgg cgagtaataa aggattacgt caatctttag cggatcaatg caaagtcaat
    13981 gacattcaat taactatccc aagtcctaaa ttatgcacag ataatgctgc aatgataggc
    14041 gttgccggcc actatttgta tcagcaaggt cgatttgctg atttagcatt aaatgggcac
    14101 agcaatatag atttagaaga gtattctgca gaataaaata aacgtcagtt attaacatga
    14161 ttactatgaa cattattgaa actgttcata gtcttctgtc atgtgatagc tgacgttttt
    14221 atgtttaaag ttaaatgcta aattttcggg acattatctt ttgctcgttc atatattttt
    14281 gctggaaatg aagtgatttt taataattcg atggcattac gtgtatttgc tttgccaggc
    14341 ttaattttgt aatcaaaatg tatgttatta ttttctatta cctcattgaa atggtaattt
    14401 tcataacgtt gttttaagag ttcagctaac tcaatatcat gtgttgctgc aataactcta
    14461 aagttagatt tttcatgtaa aaatgatagt actgattctg aagcggcaat tcgttcagtt
    14521 gtgttggtac ctttaaaaat ttcatctata aagcagtata ttttttgatt atctggaatt
    14581 tcaacaatac gcttaataga ttttaattca gccataaaat aactatcgcc tgataacaca
    14641 tcatcggcat ttgccattga cgtgaacaca attccaggtt gatatacaaa tttgcttgcg
    14701 gtaactgttt gtatagccga tgcaagaata atgtttattg ctatggattt cataaatgtt
    14761 gatttacccg aagcattaga cccagttaac agtatatttt gtgatagtga gaaatcattg
    14821 gctactgcat ctgctatgag tggatgtgtt aactcagaga atactatgcc atcatttgaa
    14881 tcatcaattt gtggttcagt ataacattct agcgttctac gatacattgc taatgcataa
    14941 tgattatcta attcagctat gtaatcgaaa caatttttaa gctcattcat atgtgttgta
    15001 tagctttttt gaattgaatg aaataaaaca taatcaagca tgaaaataag tttaactagt
    15061 ttaattacca tagctccgat atcttcatca ttaacttcag ctaaaacact cgttaaatga
    15121 cgtgctgttc taaattgctt aaaattgact tcaggttgtg gagcatgttt aatttttgag
    15181 atagtatagc cttgttttaa aacattggat gcataaaaga tagattttaa gtcatcctca
    15241 tatgtacgct ttaaagttgc tgataaaatg atattaaata ggcaactcat aagaaataat
    15301 aaaataccaa cttgtgaatt aataaatata actgcaaatg agataactgg taaaaacggg
    15361 catagaaaca atatattttg acgttttacc ggtgtgattt ggtctggaaa tgttggataa
    15421 acagttttac caatcaaagc taaatgatat gttacgtttt ttctaaattc agcattatca
    15481 ttaaataaac taagtaatga tttgttcgta attgaaagca ttccacgtaa acaggcatat
    15541 agcttcattt caccaatagc tgttaaatta taattcatct tatgaaagat tgcattcata
    15601 tttaagtcgg accaagtttt atcatcaact aatgaatgtg attcaaattt ttccttgtag
    15661 agcttatact catagtcgaa atgtgagttc ggtctgataa aagcttctaa tggagaaatc
    15721 gtttgccata atgtttccat agattttaat agacgttgac gactcatgta tcgtccaaca
    15781 attcctatta cagatgtaag tagaataatt gctataacaa atattaaaaa tgtttgattt
    15841 gtgctcataa gtatcccctt atttattttt aataataata aaaatatact tcaatattat
    15901 tattatttca taaagaaaat gtcttaacaa tgaattttga taaaaacaat gtcaatagag
    15961 aacatgcgta cttatcaaca aagagtgaat aacttgtgga taaaaggtgt tgataacgtc
    16021 atttattgtg gatatcaggt gggaaaacag aaaataaatg attgattagt gttaatttac
    16081 ttgtgggtaa ctaggataag tctgtggata gctataatat cagcgttttt attgtgaata
    16141 aaaaaattaa aatttgtaaa aaaataactc taactatagc gattttacag ttagagttat
    16201 gtatattttt atagtttttg ttgtaattct tcccatttag acatagcgtg ttctaatttt
    16261 tgttcgctat cttgtttttg gatagctaat tcgtttgctt tttgtggatt gttatacact
    16321 tctggttggg ttaattgctc gtctatttgt aagatagtag tctctaaagt ttctatttca
    16381 ttttcacact cggatatttg tcgttcaagt ttgcgttgtt cacgtctttg ttctttttgg
    16441 ttatgatacg acgattgttc catagacttt ttatgtgctt gtacgttaac tgactgttct
    16501 tcttggtgcg ctttcaacgc ttccgcttcc tcaacttttt caatgtaata ttgataatct
    16561 cctaaataca tctttccgcc atcaattgtt aaatcaaata ccttgtttgc taattggtta
    16621 ataaagtaac gatcatggga aacaaatagt attgttcctt caaaatgttg gagtgcttgc
    16681 tctaacattt cttttgaatc tatatcgagg tggttggtag gttcatctaa aataagtaca
    16741 ttgtcacgtt gcaacataag tagtgctagt agtaaacgtg ctttttcacc accagataaa
    16801 tcattaatta tctttttaac atcgtcttgt acaaataaga aacgtccaag aactgctcga
    16861 atatcttttt cattcattaa cggatattga tcccacacat aatctaaaat cgttttacta
    16921 gatttaaatt ctgcttgctt ttgatcataa taaccaattt gtaaatttgc gccgaaagta
    16981 atatcgccat taagcgcttt ttgttgatta gcaatagttt taattaaggt cgattttcca
    17041 ataccatttg gcccaatgat tgctatatga tcgcctttag agacctctat actcataggt
    17101 ttggtaattg cagtttgata accgatttct aaatttttta catgcatgac gtcattacct
    17161 gtattccggt caaagccaaa ttgaatattt gcacttttgg catctaacat tggtttatca
    17221 atgcgttcca ttttttctaa aatcttacgt ctactttttg ccattccact tgttgaagca
    17281 cgggtaatat ttttctcaac aaaagtttct aatcgtttta tttctgcttg ttgactttca
    17341 tattcttgca ttcgtttttg ataatataaa tcccgttgct gtataaattc ctcgtaatta
    17401 ccaacatagc gtttgacatc acctaaagcc acatcataaa tttgagtaac tattttatct
    17461 aaaaagtaac gatcatggct gatgatgacg attgcacctt taaaataacg tagataatct
    17521 tcaagccact ttgtcgtttc taaatctaag tggttagtag gttcatctaa aagtaataaa
    17581 tcaggttcgt ttaatagcat ttgagctaaa gataaacgtg ttttttggcc accgctaaaa
    17641 tcattgatag gtttattgaa atcttcttca ctaaaattta acccgtaaag tactgtttta
    17701 attttacttt catattgata tccttctaat tgttcaaatt gattcgataa agattcataa
    17761 cgggacatat gtgttttata tgtatcacta tcgtaatcat ttgcatgttt tgataaacaa
    17821 tctgtttctt ctttgattaa gctttccatt cgtttaatat gttcaaatgg ttttgacatt
    17881 tcttcaaaaa ctgttgcatt agaatttaaa gtcatttgtt gagttaaata acctagtttt
    17941 aagtttttaa tttttgacac atttccagaa tcatagtttt ctacgccagc tataattttc
    18001 atcaatgttg atttaccggc gccatttctt cctacgatac caattcgttc accagttttt
    18061 acttcaaaat caacatcagt aaatatatct tcaccatcga acgattttga tatatgattg
    18121 agttgtaaaa gtatcattcg atcttcacct ttcgtctttc tcaaatttta ttaacttatg
    18181 aaaaaagtcg taattattac attaataacc attttacagt ataaaacgcc gtcttgaaac
    18241 taagatattt atttttaaaa ttcaataatg tataatattt aggtattcat tgtaatcaat
    18301 tatttgtttt ttaataatct aacaattacg ctctaactcg tttgaataca tagatataat
    18361 tcattcaaac acgaaggagg aaatagcgaa tgagtgacca agttaaaatt cctcgagcaa
    18421 ctttaaaacg tttgccgtta tattatagat ttgtcagttc attaaaatct aaaggtatag
    18481 atcgtgtaaa ttcaaaagcg attagcgatg cgttacaaat tgactcggca acaattcgtc
    18541 gtgacttttc atattttggc gaattaggta aaaaagggta cggatataat atagatagtt
    18601 tattggattt ctttaaatct gaactaagcg agagtgacat gatcaaaatc gcaattgtcg
    18661 gagttgggaa cctagggaaa gctttgctca catataactt ttcaatacat gacgatatga
    18721 cgattacaga agcgtttgac gtaaaagaag atgttattgg ccagaaaata gggaacgtta
    18781 ttgttaaaga taacgatgaa ttaataacaa cattgaagaa ggaagaaata gatgttgtga
    18841 ttctaactac accagaaaga gttgcacaga aagttgcaga tgaactcgtc caagctggtg
    18901 tgaaaggtat tttaaacttc actcctggta gaattaatac gccttcagat gtgcaagtac
    18961 atcaaattga cttaggtata gaattacagt cattattatt ctttatgaaa aattacagtg
    19021 aataaatgtg atgttagctt tgaatgatta accttgttat atctttaatt aattatagaa
    19081 cagtctggga ctaagtatgt ctcaggcttt ttctgttatg tttgaaaatg aaaggaaaaa
    19141 ctgttgtgtt gataaatata gtataggtta ataaattaac acgtagagat ataatgatga
    19201 atcgtaatta atcgccttcc ttatatgaat ataatataaa tatcaaaaat attgtgtata
    19261 aggttgaaaa caagaaaatc atcacctata atacatacta aacttatcgg aaatgagagg
    19321 ttaagattat gtggatgatt tttagtggct taattgtcgg gggactctta ggttttgtca
    19381 tgcaaagaac aagattttgt ttaacaggtg gctttcgaga tatgtatgtg caaaagaata
    19441 ataagatgtt ctatgcatta ttaatcgcta ttactattca aagtatagga ttattgattt
    19501 tgacggcaac agatatttta caaattcctg cacatagttt tccaatattg ggaacaatta
    19561 taggttcttt tatttttgga attggaatag tattggctgg aggatgtgca acaggtactt
    19621 ggtatcgcgc tggtgaaggg ctaattggta gttggattgc attagtatta tatgctgtta
    19681 ctgcagcaat cactaaaaca gggattttaa agccagtaat ggataaaatt aatcaaccaa
    19741 cgaatgtaaa tagtgatatg tctcaaacaa ctggcattcc gttttgggga ttagtcgtta
    19801 tattaactat aatcaccatt tttctagttg taagaacact caataataag aaagttcgag
    19861 ttgctgttcc aaaactaaag caacgttata caggtattag atattacctt ttcgaaaaac
    19921 gataccatcc atttattgca gcaattgtaa ttggacttat cgcactctta gcttggccaa
    19981 tgagtgcatc aactggaaga aatgacggtt taggtataac aacgccttca gcaaatttag
    20041 tacacttttt gattacaggt gaaactaaat ttattgattg gggtgtcttt ttagttctag
    20101 gaattttcat tggttcatat attgcagcta gaggatcaag agaatttaaa tggcgattgc
    20161 cagacaagat tacaatacga aacagtgcca ttggtggcat atgtatggga tttggtgcgt
    20221 cagttgctgg tggttgttct atcggtaacg gtttggttga aacggcaacg atgacttggc
    20281 aaggatggat tgcgctagca tcgatgatag ttggtgtatg gacaatgagt cattttatct
    20341 ttgttcgtcc aatgaaaaaa gtacaccaac aatctgcaaa ggttaaacag caaacgcaaa
    20401 tagtatagaa gattattatg caaatgatgt tgatcaaata aaagtgattg gaaaaggaga
    20461 aataattatg atacacgaat taggtacagt aggaatggta tgtccatttc cgttaattga
    20521 agcgcaaaag aaaatggcaa cattgcaatc tggagatgaa ttaaaaattg attttgattg
    20581 cacgcaagcg acggaagcca ttccaaattg ggctgcagaa aatggttatc ctgtaacaaa
    20641 ctatgaacaa attgataatg cttcatggac aattacaatt caaaaagttt aacgttatca
    20701 ttttaacaat aaaatagata ttagattcta tggctacttc cgctaattta aaagtgagta
    20761 agtagtcttt tttgttttag ttcatgaaat catttttata tagtgtggca cattttattc
    20821 caaaagatgt aataaaactt aacgcatttt tgctttttat aaattgtcag attattatga
    20881 aaaaaaggga gtggtaagta tgaatcttaa cgatacgata tttatgtttt tgtgtacatt
    20941 attagtttgg ttaatgacac caggattaag tttattttat ggtgggttag ttcaatctaa
    21001 aaatgcgctt aatactgtca tgcaaagtat ggcagcaatt gtgcttgtta catttgtatg
    21061 gataacagtt ggttttacaa ttagttttgg gaatgggaat ttatggttcg gaaattggga
    21121 atatactttt cttaatcatg taggttttgc gactcaagaa gatattagcc cacatattcc
    21181 tttcgctttg tttatgttat ttcaaatgat gttttgtacg attgcaattt ctattttatc
    21241 tggttcaatc gctgagaaaa tgaagtttat tccttattta ttattcgtag taatatggac
    21301 tgctcttgta tacagtccag tagcacattg ggtttggggc ggcggttgga ttaacaaact
    21361 cggtgtatta gatttcgctg gaggtacggt tgttcatatt acatcaggtg tttctggttt
    21421 agtattagct attatgattg gaaaaggaaa caaacattct gaatcaacac cacataatct
    21481 tatcattacg ttgattggcg gtatattcgt gtggattggt tggtatggat ttaatgtagg
    21541 tagtgctttt acatttgata atattgcgat gcttgcattt acaaatactg tcatttcagc
    21601 cagtgcaggt gctataggtt ggttaatttt agaatatatt tttaaaaaga cgacaagttt
    21661 acttggactt ttactcggtg cattagcagg attagttgtc attactcctg cagcaggata
    21721 tgtaacatat cttagtgcaa caataatggc tttaatagga ggtatctgtt gttatattgt
    21781 cattaattac atcaaggtaa aactaaaata tcatgatgca ttagatgcat ttggtattca
    21841 tggtgttggt ggtattattg gtgctgtttt aacagcagtt ttccaaagta aaaaagccaa
    21901 tcctgacatt gagaatggct ttatttatac tggtgacata catattatac ttgtacaaat
    21961 attatgtgta acagcagttg taatttttag tatagtcatg acgtttatta ttgcgaaagt
    22021 aattaaatta attacaccat tatctgttac ggaacaagaa acgaatatag gattagacaa
    22081 gattgttcac ggtgaacatg cttactttga aggtgagcta aatagattca ataaacatat
    22141 tcgatattag aatatattta catagaatat tcattgtcct gacatttaac taaaggttga
    22201 tgttgggaca ttttgttata caaaagtttt attttgaaat ctttttatga aagaagcaga
    22261 aatattattt aaagcggtta cacatatgct aaaataaggc taagtgtcac aaataatgat
    22321 aggtgaataa gtatgaaaaa tatatctgat attgccaaat tggcaggcgt ttcaaaaagt
    22381 acagtatcta gatttttaaa taatggatct gtcagtaaaa aaacaagtga aaaattaaca
    22441 agaattatag cagaacatga ctatcaaccg aatcaatttg ctcaaagttt aagagcgaga
    22501 caaacacacc ttattggtgc cattatacct agaatgaatt catatgcagt agatgaaaca
    22561 atcaaaggat tggcaaaaca atgccaaaaa tatgaatcac aattaatttt aaattacaca
    22621 ggtttaaata tcgaagcaga aatacaagcg cttgaaacat tagcacgcag taaagtagat
    22681 ggtattgttt taatggctac agacataaca gagagacata ttgaagtcat taataaaatg
    22741 aatgtaccaa tcgttattgt tggtcaacaa catgaacaac ttcatagtat tgtgcatgat
    22801 gattataaag caggtcaaat tataggcgaa tggattggtc aacagggata tcaacaagtt
    22861 gaagtgttta gtgtaagtga aaaagatatt gcagttggta tacatagaaa acgtggttta
    22921 cttgaccagt tagctaaata ccaaattaaa cctaatatac atgaaacaaa ttttacttat
    22981 gtggaagcac aaaaagatgt tgcaaatgtt ttggaaaatg tggagcaagt agatgcggtt
    23041 gttggagcaa ctgatacgat tgcattagct gcctataaat attattctga taaaaaagat
    23101 gttatgaaac cacatcaaat atatggtttt ggtggtgacc caatgacaca attagtgtct
    23161 ccatcgataa aaacaattca ttataattat tttgaagctg gccaatgcgc gatggaagag
    23221 atacaacaga tgcttaaaaa gcaagatatg ccatatagcg tcacagtaga tgttaatatt
    23281 tagacgctgt attttttaaa ataaatgtgg aaccgatacc atataactat aaatggatag
    23341 gttaaaagtt aaagaacgta ggtaaaattt gctataatag aatataaatt gttattagca
    23401 taaattataa aaggaggact gggtaaatat tatgaccgaa tggactagag aagaacgtta
    23461 tcaacgaatc gaggacgttg atactgagta ttttaaaaca ttaaaacaac aagttgatca
    23521 atcaaaattt cgtcaacaat ttcatataca accagaaaca ggcttattaa atgaccccaa
    23581 cggacttatt ttttataaag ggaagtatta tgtttcacat caatggttcc cattaggcgc
    23641 agtacatggc ttaaagtatt ggtataacta cacgagtgat gacttaataa actttaaagc
    23701 tgaagggcca attttaaatc cagatactaa atatgacagc catggtgtat atagcggtag
    23761 cgcttttgaa tataacgggc atttatatta tatgtacaca ggaaatcatc gagataatca
    23821 ttggcaacga catgcgagtc agatgatcgc acgattgaaa gaagacggtt cagttgaaaa
    23881 gtttccaaag ccagtaatta gccaacaacc agaaggatat acgagtcatt ttagagatcc
    23941 taaagttttt aaatatgatg agaaatatta tgcaatcatt ggtgcacaaa ataatgatca
    24001 gcaaggtcga ttattacttt ataatactga agatataatt aattggcatt atttaggtga
    24061 aataaataca gagttggatg attttggata tatgtgggaa tgcccagatt actttaatgt
    24121 agataatcaa gatgtcatac ttatttgtcc acaaggtatt gaaccaaaag gcgatcagtt
    24181 caaaaatatt tatcaaagtg gttatatact tggaaagttt gatattgaaa agttaacata
    24241 tgaacatgaa aattttgtcg agcttgataa tggttttgat ttctatgcac ctcaaacatt
    24301 tttagatgaa aaaggccgac gagtactaat tggatggatg gggttaccgg aaatcgaata
    24361 tcctactgat aatgaaggat gggcccattg cttaacaatt cctcgtgtat taaatgtaga
    24421 aaatggtcaa cttaagcaac gtccatatcc agcgttggaa aaattacgtc acaataaaga
    24481 gacagcatta ggctacgcaa ataaatttac tcgaaaatta catccgtatg aaggtaaaca
    24541 gtatgaatta atcatagata ttttggataa tgatgctacc gaagtgtact ttgaattacg
    24601 tacatctaag acttcttcaa cattaattgc ttataacaag cgtgaaaata aaataacatt
    24661 agatcgcagc gacagtggtt tattgccgac aaatgttgaa ggtacgacgc gtagtacgat
    24721 attagacacg ccattaaagc aattacaaat ttttgttgat acatctagta tagaaatttt
    24781 ctgtaatgat ggagaacgtg ttttgacatc tcgaattttc ccgacagaag acgcactagg
    24841 tattaaaaca tcaacagaat cagggcaagt atatttacaa tttactaaat atgatttaaa
    24901 ggatgaacat aaatgagacg tctattttca ataggagaag cgttaattga ttttattcca
    24961 aatgttacaa atgcgaattt aaaagatgtt caaacattta cgaagcaaat tggtggcgcg
    25021 ccatgcaatg tagcttgtac agttcaaaag ttaggacaac aagcgtatat gattacacaa
    25081 ttaggtaatg atgcatttgg agatagcatt attgaaacga tatcaagtat tggtgtagat
    25141 gtatcaaaag tgtatcgcac gaatgaagca aacactgcgt tagcttttgt gagtttgact
    25201 gaagcgggcg agcgagattt ttctttttat cgtaagccct ctgctgatat gttatttgaa
    25261 ccaagttttg tgaatgatat tgatgtgaat gaaaatgatg tagtacattt ttgttctgtt
    25321 gatttggtcg atagtccgat gagagatgca cattatcaat tgattaccaa aacattaaat
    25381 gcaaatggta ctgtcgtatt tgatcctaat gtaagattgc cattatggga taacgctgaa
    25441 gatttaagac aaactattca tacctttttg ccattggcgc atattgtaaa agtttcagat
    25501 gaagaattag aatttattac aggtattcat gatgaaaatg aagccattca atcattattt
    25561 acaggtaatg ttactgtagt catttatacg aagggagcag atggtgctgc cgtatatttg
    25621 aaaaatggta ttaatcatta tcatagtggc tataaggtaa aacctgttga tacaactggg
    25681 ggcaggggat gcatttattg gtgcagttat tagcaggatt ttagcaaccg atgtgttaaa
    25741 tttaactcag ttatttgaaa atgaaggtga ggaaatttta gcgtttagca atcgcgttgc
    25801 tgcaatagtg acaacgaagt atggtgctat taatagtttg ccaacattac aagaggttga
    25861 acaagcattt taaataattc atttataaaa cagtgtagga aacaagttga atcttgtgct
    25921 acactgtttt ttaaaacaaa ataagattca cggagtagga aattatagca ttttatttat
    25981 ggaaactgtc aatttcaaat ttagtatatt ttgttaaaaa ttgcgccata ggattgtaga
    26041 gtgaaataga aattgataaa ttatgtagta ataggaatag gtatacaaag agacgttaca
    26101 tcatttttta attcacgcgt catatttaat tttgttaata aaattaaaaa gccagctata
    26161 cagtgcattt gctagttatc ttgttaaaat ccaacaagat ttacaattga atacgccgtt
    26221 aactgacttt attatcttat tatatttttt taacgtttct caccgatgca tagcagtgtt
    26281 ctttattttt aaaatagaca attcgctctt tcgaatctat agattcaata ttatggcgat
    26341 tgacgacaaa gctattatga catctaaaga aacgatcatc taattgactc agttctttta
    26401 aattaccata aaattcaatt tgacggttat ctaaatgggc aatgagtctg tgagattttg
    26461 ttgatgattc aaaaaacata atatcatcat attgaacata cactgaatta ctgccacgtt
    26521 ttaattcaat cgtttcaacg ctattatctt tagacaacaa ttgtaagcgt gtatgtgcag
    26581 tttctaaaca gtctataatt cgagttctta attcagctgg atcatcttta aaaataaaat
    26641 ccatcgctgc aactttgtag acaaatgtta aataggtaag ttcactgtga ctcgtaacga
    26701 aaataatgtt accaactggg tcatgcttac gaatttcact gcctaatttg ataccattaa
    26761 tatcagttga aagttgaata tctaaaaagt aacagcctat gtcattcata tttttagctt
    26821 gctcaagcac ctcataagga ttatcagttg cgagggcaat ttccataggc ttttcttcta
    26881 tcattatata atttttaata atggtaacca tgttttctct ttgttttgga tcgtcttcgc
    26941 aaatgaaaat tttcatacat tcacatcctt atggctagtt gttaataatt tcaacttttt
    27001 gaataaagaa accattttcg ataattgtat ctaataagac attgtctgca ttatcagcaa
    27061 tttcttttaa agttgataga cctaaaccac gaccttcacc tttagtagaa aaactttctt
    27121 ggaacaattc atgaatgcgt ggtatatcat cagcgcattt attcataaca ataaacgtta
    27181 ctgaattttc actttcaata aatgcaacgc gaatgatagg gtcatcaatt tcagttgatg
    27241 cctcaattgc attatcaaga ataataccaa tactgcgact taaatcgatc atattcaagt
    27301 taatgctact tacttcatcg ggtatttcga tactaatcgg aaaattcatt tcttgtgcac
    27361 gtaaaatttt cgcagtaatt aagcctttaa tttcacgtac tttaagattc tcgataccat
    27421 ttaattttat agcattcatt tgtaaattgt ctttcatagg tacaatattt ttattgaaat
    27481 aatcacgtag gccaggcatg tcatcttctc gaatgtattc tgaaagtgtc gttaagatat
    27541 tgacataatc atgacggaac ttgcgcattt cgttgttgat agcttcaatc ttcaatgtat
    27601 attcataata ggtttcaatt tcttcttgat tacgtttata tttcatctct ttaaggagaa
    27661 attgagaaat aacaaatgtt aatatactta aaaatata
//
LOCUS       DANJFZ010000027        27069 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.027, whole genome shotgun sequence.
ACCESSION   DANJFZ010000027 DANJFZ010000000
VERSION     DANJFZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.027"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(191..802)
                     /locus_tag="PWN45_000991"
     CDS             complement(191..802)
                     /locus_tag="PWN45_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDL0622771.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            1002..1709
                     /locus_tag="PWN45_000992"
                     /pseudo
     CDS             1002..1709
                     /locus_tag="PWN45_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1748..2686
                     /locus_tag="PWN45_000993"
     CDS             1748..2686
                     /locus_tag="PWN45_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL0622772.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(2952..3194)
                     /gene="rpsR"
                     /locus_tag="PWN45_000994"
     CDS             complement(2952..3194)
                     /gene="rpsR"
                     /locus_tag="PWN45_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL0622773.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(3246..3749)
                     /gene="ssb"
                     /locus_tag="PWN45_000995"
     CDS             complement(3246..3749)
                     /gene="ssb"
                     /locus_tag="PWN45_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0622774.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(3770..4066)
                     /locus_tag="PWN45_000996"
     CDS             complement(3770..4066)
                     /locus_tag="PWN45_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL0622775.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            4310..4501
                     /locus_tag="PWN45_000997"
     CDS             4310..4501
                     /locus_tag="PWN45_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622776.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(4587..5684)
                     /gene="ychF"
                     /locus_tag="PWN45_000998"
     CDS             complement(4587..5684)
                     /gene="ychF"
                     /locus_tag="PWN45_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL0622777.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(5696..5899)
                     /locus_tag="PWN45_000999"
     CDS             complement(5696..5899)
                     /locus_tag="PWN45_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL0622778.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(5929..6810)
                     /locus_tag="PWN45_001000"
     CDS             complement(5929..6810)
                     /locus_tag="PWN45_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL0622779.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(6967..7812)
                     /locus_tag="PWN45_001001"
     CDS             complement(6967..7812)
                     /locus_tag="PWN45_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL0622780.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    8041..8410
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8476..9579
                     /locus_tag="PWN45_001002"
     CDS             8476..9579
                     /locus_tag="PWN45_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0622781.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            9576..10736
                     /locus_tag="PWN45_001003"
     CDS             9576..10736
                     /locus_tag="PWN45_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDL0622782.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            10705..12546
                     /locus_tag="PWN45_001004"
     CDS             10705..12546
                     /locus_tag="PWN45_001004"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDL0622783.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            12543..14771
                     /gene="metE"
                     /locus_tag="PWN45_001005"
     CDS             12543..14771
                     /gene="metE"
                     /locus_tag="PWN45_001005"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL0622784.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            14814..15566
                     /locus_tag="PWN45_001006"
     CDS             14814..15566
                     /locus_tag="PWN45_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL0622785.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(15649..16830)
                     /locus_tag="PWN45_001007"
     CDS             complement(15649..16830)
                     /locus_tag="PWN45_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDL0622786.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            17369..18502
                     /locus_tag="PWN45_001008"
     CDS             17369..18502
                     /locus_tag="PWN45_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDL0622787.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(18869..19498)
                     /locus_tag="PWN45_001009"
     CDS             complement(18869..19498)
                     /locus_tag="PWN45_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDL0622788.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(19498..20340)
                     /locus_tag="PWN45_001010"
     CDS             complement(19498..20340)
                     /locus_tag="PWN45_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622789.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(20365..21078)
                     /locus_tag="PWN45_001011"
     CDS             complement(20365..21078)
                     /locus_tag="PWN45_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDL0622790.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(21075..21278)
                     /locus_tag="PWN45_001012"
     CDS             complement(21075..21278)
                     /locus_tag="PWN45_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0622791.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            21519..21908
                     /locus_tag="PWN45_001013"
     CDS             21519..21908
                     /locus_tag="PWN45_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDL0622792.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            22008..22232
                     /locus_tag="PWN45_001014"
     CDS             22008..22232
                     /locus_tag="PWN45_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDL0622793.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            22249..22950
                     /gene="tatC"
                     /locus_tag="PWN45_001015"
     CDS             22249..22950
                     /gene="tatC"
                     /locus_tag="PWN45_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL0622794.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(23042..24754)
                     /locus_tag="PWN45_001016"
     CDS             complement(23042..24754)
                     /locus_tag="PWN45_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDL0622795.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(24735..25964)
                     /gene="efeB"
                     /locus_tag="PWN45_001017"
     CDS             complement(24735..25964)
                     /gene="efeB"
                     /locus_tag="PWN45_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL0622796.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(25961..26815)
                     /locus_tag="PWN45_001018"
     CDS             complement(25961..26815)
                     /locus_tag="PWN45_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622797.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
BASE COUNT         8822 a         5113 c         3985 g         9149 t
ORIGIN      
        1 ggttgtcgaa tgtaaaaaca tattgattaa ccataaaaga gaaattattt ttgtagattt
       61 cggcataaac gtcattaaag aattttctat atgatggtgc taatcataac aaagaaagct
      121 aggcaccttt agatacctaa ctttcaaagt attagcgata aaacactata tatactaatt
      181 tagcttaatt tcaaacttgt tcaatgtcat taacactttt cacaagtttg aaagttacat
      241 tttttatatc ggctgtatta atttttacat tttccctatc tttttgtagc tctttatgcg
      301 actctaatgt atatttacca ccatcttttg tatttatgac gatgttacct ttaggcaaat
      361 gttctcccat atacaagcca taggaaatat gagcaaatct tatgatatga tccaattctt
      421 ttaaagtgac aacttcttta ttaagagtaa tattcctctt aggtgtatac actctgccag
      481 caactgtgta tgttccttct aaatgtatac gaacttggtt ttcatttaaa ggtctctctg
      541 aataaaccca attcctagat ttattagatt ttccatttat tgtatccttg ctgtatctat
      601 cgtaaagttt tttaaccaaa gctttttctg aattatttgg tacttcttca actttttgta
      661 attgtttatc ttgtacactt gaggaatttt gtgttgacgc gtctgctttt ggatatattc
      721 caaatgttcc cccatagatg atacctagcg atagaataga ttttaatacg attgaatttt
      781 ttgagtcata ttttttgaac atatttaatt acctccttga tgtaaagctt tatttgctac
      841 aattataaaa ataatagacg tgttcatgaa ttaaattcat cttaactctt gattaacttt
      901 aatttgctac cactctgaat ttaataacta taaatcgtct acacataatt ggacaaaatc
      961 taagagaata aaatttgtta atttaaaata gcaagcaatt caaagttata tgtgtaatag
     1021 ataaaataga tatccctata gtgatgcgtt actagctaaa cataataaca cattagaaga
     1081 taatgaagtt aaggagttac tggattgttt cgactatgta attaagtata aaaatatcca
     1141 acgacaaaac gtaattataa aatggtaaaa gctatggtac agtttcaaat tgctaatgac
     1201 atgcgtatcg gtgagctact tgcaataaag agagtaaata taaactatga agataaaacg
     1261 ctagatatcg acggtaaagt taattggata actgaaaaaa gacgggagca ttcggagtaa
     1321 aggagacaac tgaaagaagt aatagctata aggccacagg gctcactacc caaagcatcg
     1381 acttactaag aacacttatg cttggaatga taagtttatt gatagagagt acatattcac
     1441 aaatacgtct ggtagcccta tcgactcgaa caaaattagc cacattatta aaggggggcg
     1501 ctgatattag ttctattaag aaacctataa cgacgcatac attacatcat tcgcatatat
     1561 ctacacttgc tcaattagga attaacttaa aagcaatgca agagcatgta ggtcattcag
     1621 attataaaaa aaatctagag atatacacac atgttactaa tcagatggcg aaagatatga
     1681 tgaataaatt tgaacgattg gggagttaaa attggaaaaa gatgatacac tagcagaaat
     1741 taagcctatg ctcaattttg atgagcaaat agcgaaatta aaacagatga atatattttt
     1801 taatattatt gacaccgaaa aagcaaatga aattcttaga aaaaataatt acttcttcaa
     1861 actagcttat ttccgaaaaa atttcgaaaa aaagaatggc ggctatttca tagaatttgc
     1921 ttatttatca gatttagcaa ctatagatat gaaattaaga tacacaatgt tgcatttaac
     1981 tttagatatt gaacatagtt taaagtatct agtcttaaaa ctaataacag aaaataacca
     2041 agaagatggt tataaaataa tagatgagtt cttatgtatt gataaatcat atagcaattc
     2101 aaattttgac acaaattcaa gaacaccaga agaagttatg gaaaccaaaa tcaaaaataa
     2161 aaacgaaata ttcaagcata tgaataaacg aggacaacta cccgagaagt tgaataaata
     2221 ctatcaaaat ccacccgcat gggtttgcat tgaattcatg caactaggtc aattcgtttc
     2281 gtttctcaac ttctattaca agaagtacaa tgacgaagaa ttgagagttg ctaatatttt
     2341 aatgccttta gttaaaaata taagaaacaa atcagctcat aaccaaccca tcatagcaaa
     2401 tctaaattat gacagtagat tacctcaata tttatttgaa aaagggaata atataggcat
     2461 atctagaaac atgttcggaa taaaaaattt catagatact ttcgctacgc tagaattaca
     2521 taatcaagtt tgtagtaatg caattatcca agcaagatat cacgatttgg accaacttca
     2581 aaagcgatat aaaagaaacg aaagctatta taataatgca ttagctatca aaagattttt
     2641 tatagcttta gataaaatta ttgacttcaa cagaccaaaa gtataaacta tctagtgagg
     2701 aaagagactt ataggtctcg cgagttattt taattcgtat gcaagaaaaa gaagagctat
     2761 gcattttatt taaaatgcgt agttcttttt ttatgcatct aaattcatat tatttttgca
     2821 atataaacat atctttgtgc aaattccgaa cacaaaacat tcacatcatc cttttttgcc
     2881 ctttttctat accccaaaac acaaaaagcc ccgtaagcct atgcctacgg ggtttgacaa
     2941 taaattatat attattgttc ttctttaaca tatggtaata atgccatatg acgagaacgt
     3001 ttgatagctg tagtcaacat acgttgatat ttagctgaag taccagttac acgacgtggt
     3061 aaaattttac cgcgttctga gataaaacgt tttaataatt cagtgtcttt gtagtcgata
     3121 tgtgtaatac catttgctgt gaaatagcat acttttttac gacgacgtcc gcctcttctt
     3181 ggtccacctg ccatgattgt gtgcctcctt tgataatttt ttcgctttaa tttcgttaat
     3241 ttttattaga atggtaagtc atcatcactt atatcaatcg gtccgtttgc atttgcaaat
     3301 ggattatcag attgtttcgt gtttgatgaa ttattgtacg aattgttttg tcctgattgt
     3361 tgaccaccga atccttgacc gtaatcttgg aattcatttt gttgacgttg gccaccattt
     3421 tgttgcgcat ttttaggttc aaggaattga acgctatcac acacaacttc agtaacaaac
     3481 acacgacgac cttcttgatt ttcataatta cgggattgta agcgaccatc tacaccagct
     3541 aaactacctt tagataaata gttatttaca ttatctgctt gtcttctaaa aacaacacag
     3601 ttaataaaat ctgcttcgcg ctccccttga gcattcgtga acgtacgatt tactgcaaga
     3661 gtgaatgtcg ctacactcac acctgaggga gtggttctgt attccggatc tttcgttaaa
     3721 cgacctacta atacaactct atttagcatt taaacgcccc ctctaattat tacttgtctt
     3781 cgtcttcacg aataaccatg taacgaatga tatcgtcact gattttagct agacgttgga
     3841 attcgtcagt agctttgttg ttatcagatt taacacgtac gatgttgtag aagccatctt
     3901 tgaaatcatt gatttcataa gctaggcgac gtttacccca gtcttttgct tctaaaactt
     3961 ctgcaccttc agtagctaag ataccgttga aacgttcaac taacgctttt ttagcatctt
     4021 cctcaatgtt tgggcgtacg atgtacataa cttcatatgt tctcatttta tatttgcacc
     4081 tccttgtggt ctatacggct tatcaatctt aaaacagata agcaaggaat aattttcatt
     4141 actcacaata aagaattata tcatgcgcca ttacttttta caataataat tcaaactact
     4201 cttcatatca tttttgatat taattcattt gaaactttct atgatatttt taaaaataca
     4261 cttcacaaaa gcgaacatat gttctataat agttgtgagg tggtaaggaa tgaatttaaa
     4321 tattgattgg tctaaagatt ttcaagaatt ccaagagata cttaatagtg gtattcatcc
     4381 tgaatggctt tattgtgcaa aggctaatct tgttttagag cctgcttata ctggcgaagg
     4441 caaacaattt ttcagcactc aagatattat taacgcgagt aaaattattc cattctttta
     4501 ataaattata cgcataatta aaagaggcga acaattgatt ccgcctcttc tacatatata
     4561 ctatttaatt aacttcactc tatcgtttat acattaaatc tgaaatgaac gatatcgcca
     4621 tcttgcataa tatattcttt accttctaat cgttgtctgc ccgcttcttt agcgccactt
     4681 tcgccaccat attgtacata gtcgtcataa cttgttactt cggcacggat aaatccacgt
     4741 tcaaaatcag tatgaatgat accagcacat tgaggtgcag tcataccttg tttaaatgtc
     4801 caagcacgta cttcttgcac accagcagta aaatatgttg ataatcctaa taattcataa
     4861 gttgttctaa ttaatcgatc taatcctggt tcttcgatac ctaaatcttc taagaacatt
     4921 tctttatctt catcatctaa tgtagcaatt tcttcttcaa tttttgcact aataacaatc
     4981 acttcagagt cttcttgcgc tgcatattca cgaatcgctt ttactttatc attatcatca
     5041 tcaccaattt catcttcacc aacattagcg atataaagca tttttttaga agtcagtaat
     5101 tgcgcttgat tcacccattt ttgatcttct tcattaaagt caatactacg agcgggttta
     5161 ccattttcta aagcttcttt aatagttgtt aaaatacgta cttccatttc agcagtctta
     5221 tctttttgac gtgctaattt ttcaattcta ggcaaacgtt tctcaacaga ttctaagtcc
     5281 gctagtacta attccatatt aataacttca atatcatcaa tagggtctac tcgaccagca
     5341 acatgagtta cgttatcatc atcaaatgca cgaacgacct gacaaatcgc atctacttct
     5401 ctaatatgtg ataagaattt attacctaac ccttctccct ttgaagcacc tttcacaata
     5461 ccagcgatat ctgtaaattc aaatgtagtc ggcaatgtct ttttaggttg aaccatttct
     5521 tctaatttaa gtaatctagc atctggcact tctactatcc ctacattagg atcaatcgta
     5581 gcgaatggat agttcgctgc taaagcacct gcttttgtta ttgcattaaa taatgttgat
     5641 ttaccaacgt ttggcaatcc aacgatacct gctgttaaag ccattaatca ttctcctatc
     5701 tttgtgtatc atcatgagat tcgatgattt ttttaagttt tttatcaaac gtttgacgtg
     5761 gaatcataat acttctttga caattttcac atttaattct tatgtctgca cccattctaa
     5821 taatcttaaa acggtttgtt ccacacgcat gttgtttttt catttctact atatcattta
     5881 ttccatattt tgacgccatt aaatatgacc tccatgtatt ataaactact aaacaccgtt
     5941 ttcactatga ttataagcag tcataattgg ttgaggtgtt ttaatacctt cttgtaaaaa
     6001 cattttttgt acttctttgc gaataattcg agctccagca aatccttcac ctggaattgt
     6061 ttctgcagat attctcaata ttactcttgt atcttcaata gcatcaatac caataacaac
     6121 cggatcacta acaaataagt aatatttact acgtaaagaa gtaaatagtt tgtttagttt
     6181 tttatcaaca ttatcaatgt tttcttcgac agacactgga attttaacga tagctgtacc
     6241 atttgtaatt gagtagttcg ttatttcacc catactacta tttggtaaaa ttgttaattc
     6301 tcctgaaatt gtattgattc gtgttgaacg aagtccaatt gatttaactg ttccctctgc
     6361 tacagttgta ccaccgttat ttatcttaac ataatcacct acatcaaatt ggctttcaaa
     6421 tataataaag aatcctgtaa ttacgtcttt aactatagtt tgagcaccaa aacctactgc
     6481 taagcctacg acaccagcac tggcaattac cccttcaaca ctaatgccaa atttacttaa
     6541 aatcgtcgta ataactataa accaaacgat atacttcact acattttgaa caagagatat
     6601 taaagtttta gagcgctttt tgttactctt tttactttta ttttgaatct taaatccctg
     6661 ttcaatcatt ttattcagta ttttaataac tatgagggct acgataatat aaataacaat
     6721 catagcgatt ttagttgcaa tgttttcata tgtttctatt tttgttaatg gctcaaatag
     6781 agatgaaata atattcatga cttgattcat gataagcgtt actcccttct atatgatata
     6841 tcaatattgt attcatattt ttatatactt ccaacctacg aaagtctcaa tatatctaga
     6901 atcaaatgtt cactgattac aatttattta cacttgcaca aagagttatt atataaaatt
     6961 gtgtaactat ttaccatacc tacgatttaa ttgttcaatt attctcacaa aatcttcttg
     7021 tgaatcaaac tcaaatgaga ttttaccaac cgattttttt attgatatat ctactttggt
     7081 accatactgt tctcgcaact gtcgttcttg ctgctttata aatttaggct tagttatatc
     7141 tactttgtct gtttccgact ttgacgaaac attttttaat tcattaacat ggttttctaa
     7201 atatctgaca ctccactttt ctttaacaac ccgtttcgct aacctaagca tttgttgttc
     7261 atctttaatt gccaataacg ttcgtccatg tgcacttgtc agtcgcccat cttttaccat
     7321 gtcagcaatc tttttcggca aatgtaataa cctcaacata ttcgctatat acgggcgcga
     7381 cttactcaat cgtttcgcta cttcttgttg tgtaattttc aaatctgtca tcaaacgttg
     7441 ataactttca gcttcttcaa tcgcatttaa gtcttctcgt tgtaaatttt cgatgaccgc
     7501 cagttccatc atatcttcat ctgttaaatc tttgataatc gctgatacgt attttagacc
     7561 agcaattttc gaagctctaa accttctttc accaactaca atgtaataac cttgaactgt
     7621 ttttctaagc acaattggtt gcaaaattcc atattgctta attgaatctg ccaaatcatt
     7681 taaatgattt tcatcgaaag tttttcgggg ctgatacgga ttcggtttga tatccgatat
     7741 atctatttgc ttaatttgtt catcttttgt tttagtaata cgttgatctt cactttttga
     7801 caattcactc acatcacttt ttatttgata acaacagtta aatatatttt atgtattcta
     7861 aatgagtcag acaaccatta accacacttt atttaaaaaa taattcattt ttcaacttca
     7921 agataccaat tacaatttca caaacaaatt aacttttaaa tcaaaatttt ctgaataatt
     7981 gttgacaaat ctttttttac tctgtaagct aattaatatc ttataacagt ttaatgaaac
     8041 gtaaacacaa taaagaggaa agtaaaacac accctgctta tacagagagt ctttagtagc
     8101 tgagagaaga ttttgaaagc gtgtttgaaa atggccttgg agtgttgatg ccaatatgag
     8161 gtgtctacgg gttcgcccgt tatagcgata cagtattaac attgatgtta aatggcgtac
     8221 tggattcttt acgcacgatt ttttgttaat aagtatggga tagcacatta ctatatcctt
     8281 acttactgac tttaattgtg ataattgttc agtaagcata tttactttta atgcgtactg
     8341 aataaggtta tttcagcgat ggaataacaa ataaaggtgg taccgcgaaa cataagcttt
     8401 cgtccttttt atccgattca ttcgggtacg aaggacggaa gcttttttta ttttttctta
     8461 taggagggtc ttaatatgaa ggatacacag ttagcccaaa tcacattaac cgatgattca
     8521 accggtgcta tagcgaatcc aatccattta tctactgcct acaagcatcc aaaactagga
     8581 caatcgacag gttttgatta tacacgtact aaaaatccta cacgctcaac atttgaaacc
     8641 tgttttgcca aacttgagca tggtattgca tcattcgcta catcaagtgg aatgtcagcc
     8701 attcaattaa tatgtaatct atttaaacct catgatgaaa ttttagtttc attcgattta
     8761 tatggtggca catttagatt atttgaattt tacgagcaac aatacgatat caaatttaag
     8821 tacgttgatt ttacagatta tgaacaagtt gaaaaagaaa tcactgataa aacagttgca
     8881 ttattcattg aaccaatatc taacccacaa atgattgcta ttgatgtaaa gccatactat
     8941 caactttgta aagctaaagg cttattgtca attatcgaca atactttttt aacaccttat
     9001 ctttcaacac cactagcaga aggtgctgat atagtcttac attcagccac gaaatatatt
     9061 ggcggacata acgatgtact agcaggtgtc gtaaccgtca aagatgaatc actcgcgcaa
     9121 cagttgtttg attttcacaa catgactggc gcaacacttt caccaataga tagttatttg
     9181 ttgttacgtg gacttaaaac tttgcattta cgcattgagc gtgcgcaatc aaacgctaga
     9241 aaacttgcta aaaaatgtca gtcacttcaa gcaattgacg aagtactata tagcgggcaa
     9301 actggcatgc ttagtttaag acttaacaag gcctatagcg tcgctaaatt attagaaaat
     9361 ttagacattt gcatttttgc agaaagttta ggaggtactg aaacattagt gaccttccct
     9421 tacacccaaa cacatgttga tatgccagat gctgaaaaag ataaacgtgg cattgatgag
     9481 tatttaatcc gcttatcact aggtgttgaa aattatgaag acatcgaacg cgatatcatc
     9541 caagcattag ataaagctca gattggagag attgtatgac actttcaaaa gagacagaag
     9601 tgatattcga ttggcgtaga ggtgtggaat atcattcagc taacccacca ctctatgatt
     9661 cttcaacatt ccatcaaaca agtcttggtg gcgatgttaa atatgattat gcacgaagtg
     9721 gcaaccctaa ccgtgaactt ttagaagaga agttagcacg attagaacag ggaaaattcg
     9781 cttttgcttt tgcatcaggt attgctgcta tttcagcagt acttttgact ttcaaatctg
     9841 gtgatcatgt catcttaccc gatgatgtat atggcggtac ttttcgcctc actgagcaaa
     9901 ttttgaatcg atttaatatt gaatttacaa ccgtagatac tacaaagctc gaacaaatcg
     9961 agggtgccat tcaatcaaac acaaaattaa tttatatcga aacaccatcg aatccctgtt
    10021 ttaaaattac tgatatcaaa gctgtttcta aaatagccga aaagcatgaa ctactggtag
    10081 ctgtggacaa tacatttatg acaccgttag gtcaatcacc tttattactt ggcgctgata
    10141 ttgtcattca tagtgctaca aaatttttaa gtggacatag cgatttaatt gctggtgctg
    10201 tcataactaa taatgaggca attagtgacg ctctttattt aatacaaaat ggtacaggca
    10261 atatgttatc tgctcaggat agctggacac ttgctaaaca tttaaaaact tttccaatca
    10321 gatttaaaca atctgtcgaa aacgctcaaa aaatcgtgtc atttttaata aagcaagatg
    10381 aaatttcaga agtttattat ccgggactca ctactgctca tttagaacaa gctaaaaatg
    10441 gcggtgccgt tattggcttt cgtttagctg atgagtctaa agcacaacaa tttgtcgatg
    10501 cactgacatt accactcgtt tcagtgagtc tgggcggtgt tgaaacgatc ctttcacatc
    10561 cagcaacaat gtctcacgct gcactacctg aagaagtgag acaagaacgt ggtatcactt
    10621 tcggtctatt ccgattaagt gttggtctcg aagatcctga tgaactcatt gcagacatca
    10681 aatacgcatt aaaggaggca ttcaatgagt caattcctca cacaattgaa agataatgtt
    10741 ttagtagctg atggcgctat aggaaccatt ttatactctg aaggattaga cacctgtcca
    10801 gaagcatata atcttagcca tccagataaa gttgaacgca tccatcgttc atatattgaa
    10861 gccggtgctg atgtcattca aaccaatact tatggtgcaa attttgaaaa gttaaaacga
    10921 ttcggtcttg aagataaagt taaagcaata catcaagccg ccgttcgcat cgcaaaaaaa
    10981 gcagcaaata aagatacgta tatattaggc acagttggtg ggtttagagg tatcaaacaa
    11041 gaggatatca gcttacaaac tattctttat catactgaaa ttcaaataga caccttaatt
    11101 gaagaaggcg ttgacgcgct acttttcgaa acgtattacg acctagaaga gttaacaaat
    11161 gtcatttcac gaacgagaaa gaaatacgac attccaatca ttgctcaatt aaccgcttca
    11221 aacacaaatt acttagttaa tggtcaggca atcaatgaag gattaaaaca actcgttcaa
    11281 tgtggtgcaa acatcgtggg actcaattgt catcatggcc cgcaccatat gcaagagtct
    11341 ttcacacata ttgaattacc agagcacgca ttcttatctt gttatccaaa tgccagctta
    11401 ttagatattg aaaatagtga atttaagtat agtgacaatg cacaatattt cggtcaagtt
    11461 gctcaaaatc taattcgcga aggtgttcgt ttaattggtg gttgctgtgg tacaacgcca
    11521 gagcacatca aatttattaa agaatctatt cagacactta aacctgttaa tgacaaaaaa
    11581 gtgattccga taccaacgaa agcacttttc aatccatctc aaaataaagt tagacaatca
    11641 ttaacatcta aggttcaaga acgtccaacc gttattatcg aattggatac accgaaacat
    11701 ttagacacgg atagattttt tgaaaatatc gctaaacttg ataaagctaa tgtagatgcg
    11761 gtaacactcg cagataattc attggcaact gtcagaatta gcaatattgc tgctgctagc
    11821 ttaattaagc aatattacaa tattgaacca ctcgtacata ttacatgtcg agaccgaaac
    11881 ttaatcggct tgcagtccca tttacttgga ttatcgctca ttggcgttaa cgaaatatta
    11941 gccataactg gtgatccttc aaaagttggt cacttaccag gtgcaaccaa tgtctatgat
    12001 gttaattcta aaggattaac tgaactcgct ctaagattta atcaaggtat taacactgac
    12061 ggtgatgcgc tgaagaaacg tacacacttc aacatcgctg gcgcctttaa ccctaatgtt
    12121 cgtaaattat atggtgccgt caaaagatta gagaaaaaga tagaaagcgg aatgtcttat
    12181 tttataacac aacccgtgta cagcaaagag aaaatcattg aaatttacca tgccactaag
    12241 cacttgaaca aaccattttt cataggcatt atgcctatcg caagttacaa aaacgcactc
    12301 tttttgcata atgaagtgcc aggaatcaag atgtcagatg aaattttaca acaatttgaa
    12361 gcagttaaag atgataaagc caaaacacga gaactaagtc ttaagctttc aaaggattta
    12421 atcgatactg ttcatgaata ttttaatggt ttatacatta tcacaccgtt tcaaaatgtc
    12481 gaagattcat tagaacttgc agcatactca aaatctatta ctgctcacaa ggaggcaata
    12541 ttatgacaac aattaaaaca tcaaacttag gattcccaag attaggtaga aaaagagaat
    12601 ggaaaaaagc catcgaaagt tattgggcaa agaagatttc taaagaagaa ttagatcaaa
    12661 cattaactga tctacataaa gaaaatttat tattacaaaa gtactatcac ttagatagca
    12721 tcccagttgg tgatttctct ttatatgacc atatattaga tacgtcatta ttattcaaca
    12781 tcatccctga acgtttccaa ggaagaacta ttgatgatga tttattattt gatattgctc
    12841 gcggtaataa agaccatgtt gcaagtgcac ttataaaatg gtttaacact aactatcatt
    12901 acatcgtacc tgaatgggac aatgttgagc ctaaagtgag tcgcaatgta ttattagatc
    12961 gctttaaata tgcccaatct ttaaacgtta acgctcaccc tgtcattgtt ggtccgatta
    13021 cttttgttaa attatcaaaa ggcggtcacc aaacttttga agaaaaagtt aaaacgttat
    13081 taccacttta taaagaagtg tttgaatcat taattgatgc aggtgctgag tacattcaag
    13141 ttgatgagcc aatcttagtt acagacgaca gcgaaagcta tgaaaatatt acacgtgaag
    13201 cttatgacta tttcgaaaaa gctggtgttg ctaaaaaatt agtcattcaa acatactttg
    13261 aacgtgcaca tcttaaattc ttaagttctt tacctgttgg tggtataggt ttagactttg
    13321 tccatgataa tggctataac cttaaacaaa ttgaagctgg agattttgat aaatcaaaaa
    13381 cattatacgc tggaattatt gatggtcgta atgtatgggc aagtgacatt gaagctaaaa
    13441 aagtcttaat cgataaattg ttagcacaca ctaatgaact tgtcattcaa ccatcatctt
    13501 cattattaca tgttccagta tctttagatg atgaaacatt agatacaagt gttggcgaag
    13561 gcttaagctt tgcaactgaa aaattagacg aattagatgc attgcgtcgc ctatttaatc
    13621 aaaacgatag tgttaaatat gataaattaa aagcacgtta tgagcgtttc caaaatcaat
    13681 cattcaaaaa cttagattat gatttcgaaa gcgtgcgtac ttcaagacaa tcaccattcg
    13741 cgcaacgtat tgaacaacaa caaaaacgtt tgaacttacc ggatttacca acaacaacta
    13801 ttggatcatt cccacaaagc cgagaagttc gaaaataccg tgcagattgg aagaacaaac
    13861 gcattacaga cgaagcatat gaaacattct taaaaaatga aattgctcga tggattaaaa
    13921 ttcaagaaga cattggctta gatgtattag ttcacggtga atttgaacgt aatgacatgg
    13981 ttgaattctt cggagaaaaa ttacaaggtt tcttagtaac taaattcggt tgggtgcaat
    14041 catatggttc acgtgccgta aaaccaccaa tcatttatgg tgatgtaaaa tggacagcgc
    14101 ctttaactgt tgatgaaaca gtttatgcac aaagcttaac agataaacca gttaaaggta
    14161 tgttaactgg acctgtaaca attctaaact ggtcatttga acgtgttgat ttaccacgta
    14221 aagtcgttca agatcaaatt gctttagcaa tcaacgaaga agtattagca cttgaagctg
    14281 caggaatcaa agttatccaa gttgacgaac ctgcattacg tgaaggctta ccattacgct
    14341 ctgaatatca cgaacaatat cttaaagatg ctgttttatc atttaaactt gcaacgtctt
    14401 cagttcgtga tgaaactcaa atccatacac atatgtgtta ttctcaattc ggtcaaatca
    14461 ttcatgctat tcatgactta gatgctgatg ttatttcaat tgaaacatct cgtagccatg
    14521 gtgatttaat taaagacttt gaagatatta attatgattt aggtattggt ctaggtgtat
    14581 atgatattca tagtccacgt attccaacaa aagaagaaat tactacagca atcaatcgtt
    14641 cactacaaca aatcgaccgc tcattattct gggtaaaccc tgactgtggt ttaaaaacgc
    14701 gaaaagaaga agaagttaaa gatgcattga ctgtgcttgt gaatgctgtt aaagctaaac
    14761 gccaagaata atcgcagcat ttatcatata ataaactaaa aacgggtgat tttatgacac
    14821 aatatccttt atggcaacaa ttaaatactt taaaacaagc aacttgggtt gatttaacgc
    14881 atacatttga tccggaaata ccacgtttta gtgagtttga aaaaggcgaa gtttcaactt
    14941 tattcacggt taaagatcat ggtttctatg tgcaacgttg gaatattgta acgcaatatg
    15001 gtactcacat tgatgcacca attcactttg tcgagaataa acgttattta gaagacattg
    15061 atttaaaaga attggtattg ccgttaattg tattagactt ttcaacagaa gtagcgaaca
    15121 acaatgattt catcgtaaca cgtgcgcata tagaagcatg ggaaaaagag catggtacta
    15181 ttgaacctgg cacttttgtt gcacttcgta ccgattggtc aaaacgttgg cctaatattg
    15241 aaaagtttga aaacaaagat gcgaacggac aacaacatgc accgggttgg ggattagatg
    15301 ctttgaagta cttaattgaa gaacgtcgtg ttgaagctgt tggtcatgaa acattcgata
    15361 ccgatgcatc tgtggacgtt gtaaaaaatg gtgatttagt tggcgaacgt tatattcttg
    15421 gtcaggataa ataccaagtc gagttattaa ctaatttaga tcaattacca actcgtggcg
    15481 ccatcattta cgctatcagc cctaaaccaa aagatgcacc tgggttccct gttagagcat
    15541 ttgcaattaa accatcaaat gactaatatt acccaataaa tccataaaca aataaaaaca
    15601 ggttatgcca acatgtaatt aatactgttg tgataacctg ttttcttatt atttatactt
    15661 tgatacaact gcagcgatag cttgaccgcc accaatacac aatgatgtta aaccagtttc
    15721 aacttcatca ttcagttgat gcaataatgt cactaatact ctagcaccag atgcaccaat
    15781 aggatgtcct aatgcaatag cgccaccttt aacattcacc ttttcaggag gtaattttaa
    15841 ttcacgatca acagctaatg attgtgctgc aaatgcttca tttaattcaa atacatcaat
    15901 atcgcttaat tcttttttac tacgtttcaa agccttttct acagcgccaa ctggtgcaat
    15961 acccataata gaaggatcta caccatgact tccaaagcca tcaagcactg ccaatggttc
    16021 gatatttaat tctttagctt tgtcttctga catgactaac atcatcgcag caccatcatt
    16081 gattcctgat gcattacctg ctgtaactgt accgtctttt ttgaaagctg gtcttaatcg
    16141 acttaatttt tcgactgata cattttcacg tacaccttca tccttagtga ctacgattgg
    16201 ttcaccttta cgttgaggaa tcgatactgg aactatttca ctatcaaatt caccattttg
    16261 ctgtgcacgt actgcttttt gttgtgagtt tacagcaaat gtatcttgtt cttctcttga
    16321 aataccatat tgctctacta aattttcagc agtaataccc atatgatatt gattaaatac
    16381 atctgttaaa ccatcatata ccatgctatc aaccattgat tgatgtccca ttttaaaacc
    16441 aaagcgactg ttgttgacaa gcattggtga ttgagacata ttctccatac cgccagctag
    16501 cacgatgtca ttttcaccag tcacaataga ttgatatgct aattgaatcg actttaaccc
    16561 agaaccacat actttattca ccgtaaatgc aggtactgtt tctggcaagc cacctttcat
    16621 agcagcaatt cgtgctggat tttgtccttg tcctgcttgt agtacgttac cgatgataac
    16681 ttcatcaatc tcacttggat tcaaacccgt ctctttaata atatgttcta ttaaagtcgc
    16741 acctaaatca taggctggca cgtctttaaa cgcacctcca aaaacgccaa taggtgtcct
    16801 gtatgctgct gctaatacga ctctcgtcat gtcatattca tctccttatg atatatacaa
    16861 ttttattact aatacgtcta atgaaccaat acgccactaa catccaattt atgaaaccaa
    16921 ttgctacgta tgttacattt tcattcttgt tatgcattta acaaatcata ttatacaatg
    16981 atgtaatttg tttgggcaat cggaaacgta aacggtttca ctttaacatt tcaccctctt
    17041 aaatatgctc tatcatcctc atctatagta caaaattatg ctttgattta ccacaaatta
    17101 tcattaatca tttcgcgtaa tgactctaat taaattctat ctatcattgc atcgctattc
    17161 acagctggtt tcaccatcaa tttctcatca attttttatt accatgacgt ctccagtcaa
    17221 atattaaatc gctgatgatt gttaattccg caattacctt gcagtcacct ctccttccac
    17281 ttcatcgcca aatctgcccc tattcactag aaaaaataaa tagtatactt agtaaattaa
    17341 ttacacatct taaaggagtt aaaattttat ggttaatgca ttaacaccaa aacatttagc
    17401 tgaaaagcgt gcaggattcc atgagttgtt ctttgattta attttcgtct atgccattca
    17461 aaaaattgca catgtgattt taacaacaca aaacggttcc atttcagctg atttattttt
    17521 caaatacatc gtcatgagtt tgttcttatg gttaatgtgg tctcatcaaa cattttttac
    17581 aaatcgattt ggtcaagtta cattcaaaga cgtctcgttt atgatgttta atatgttcat
    17641 catggtcttc ctatctaaca gcctatatcc ggactttgaa aagacattct tcccattctt
    17701 cttatgcgtg gcaattatgt atttaagtat cggattacag tatttattac atatacgtac
    17761 agggttagat tatggcgata agcgtacatg tcaagctttt gcgagcgtag cctttgtgat
    17821 ttcattttta tcatttctat cattagtact accacaaagt atccattaca tccctggatt
    17881 tttaggcgta ttcattgcag cgacaggact tattccattt cagaaatatc ttgtattatc
    17941 acctgttaat atgatgcatt tagtagaacg tttttcgtta ctaacaatta ttatctttgg
    18001 cgaggtactg gttggtttag cttcgtcatc atttagcatt gatcatttta gttacattta
    18061 tatattccaa tttatgattt taattagtct tttcggtgta tattggatca ttactgaaaa
    18121 ttatattaat cataaattaa catctatcgg tttccgttta tcgtatacgc atctattaat
    18181 caatatcgct ttaggtgtga ttaatgctgc aattgttttt agtaataaca ataaactaaa
    18241 tgacttgttt gaaattaata tgatgtatat cagtgtttta atattctata ttggattatg
    18301 gttaatcacg ccttatttcc ataatgaact gacaaacgcg aagtatattt catattcact
    18361 aggtatactc gttatgtctt atattgtcag ccttttattt aaaggtcacg accatgtaat
    18421 gattatctca gtatcagttg caaccttctg catcatgctg atttatttta aaaatcaacg
    18481 tctacgtaat aaacatgaat aaatagtatt tacctcggtc aaagtattgg aactgaatcc
    18541 aaaagtgtcg aacttcacat tatacttttg tgacaaactt cagttatgct acatgatcga
    18601 ggtttttaaa tatttccgag gaaatcgtta gcctatacaa tcatcaaatg atacttataa
    18661 ataacatata tcctctaatc cccactatct aaaaggaaat aattcccaca caacataacc
    18721 acctcaaaac aaataaatca atatagatat gtcacctata cttatccccc acatttcatt
    18781 ccacaccaaa aagaacagat gacaatcgta aagatttatc acctgctcta aatattattt
    18841 caaatactat catcaccaaa atcgacaatt atagttcttg tttgttaaaa attttaatag
    18901 acaatatata agaaacgctc gttataacta taccaatgac cgcatatgct atgacaacta
    18961 gccatccttc agtgctaatt ttttcaaatt ctaatgcaga tgcaccacta accataccat
    19021 atataaagaa aaagaccacg aaagaaataa gtagtagtat agatgcacat atcataatga
    19081 cattggagtt ttcagcgcca aatttaaatg tcaatgggaa aatgatagag tatgccccta
    19141 tgataccaaa actcattaaa cctgacataa taccaatcat cacactttgg gtcacaatcg
    19201 tagtcacaac taatccaatc attaaacttg caccgaataa gattaaataa aaggcaaagt
    19261 atgacttaat ataatcacta cgtttaaccg gtaaagtaga tacataatac atccatcttg
    19321 agtctttttc atgtttaata ttatcagtaa taggtgtgat taacataacc ccagccattg
    19381 ccgaactcat caacggatta aatactgcaa agtatcctgc agctataata gcgactataa
    19441 aataaatata tgtttgcttt ctcgttgcat aaaaactact taggaacata cctttcatta
    19501 tacttcacct cgcattatga tttttgtagc gtcatcaatg tgttttaaag gctctgcatt
    19561 ttcaatttct gcataatctt taactaaaat ttgatatttc ccctttgaca atctagaagc
    19621 aatgattaaa tgcttaggaa tttcaacatc tttatcttct gtcgtaacaa ttccatattg
    19681 tgctaaaagt atatctttct gttcagttaa aatcattcgt ccatctttca taaacactaa
    19741 tttatcagct aaatgttcta tatcttcaga aatatgcgat gatattaaga tgcctccacc
    19801 ttgtgcgaca aaatcttcta gtatttccat tacttcttct cttccagaaa catccatacc
    19861 tgcagttgct tcatctaaga ttaataactt cacatcatga gaaagcgcaa tcgttaaagc
    19921 tatcttcatt cgcatccctc ttgaaaaagt tttaatttta gtttgtagtg gtaactcgaa
    19981 atatttgatt aaatcaaaga atttttgact attccaagtc atataaatag attgaaatac
    20041 tttatcaata tctttaatag tcaatttatt aggcactctc aaatcatcaa acactacacc
    20101 tatgtgttct ttatattcga catcatccac ggtatgttct tctccaaaaa atgtgatact
    20161 accgttatct ttatgtcgat tgcctactag aatattaatt aatgtcgatt tacctgagcc
    20221 atttttccca attaatccaa ctgtctcatt agtagaaatc gttaaagatg catcttgcaa
    20281 tttaaaatcc gaatctttgt attgtttgtt cacactatct acgtttaaca atgttgtcat
    20341 atccatgctc ctctttgttt aattttaata aaaacgtctt ctcttcaata agtaactaaa
    20401 ggcgttatat atgaaaatag caatgataag tagcaaacta aagctttgat taattccagt
    20461 agtaattgaa taaagcccaa ttactacaat ggctagtatc aatattgata agttgatcgc
    20521 atatgtttta aataatgcaa ttaattctat atggcgttca ccttcatcca atatttccaa
    20581 tcgcttttca gtgtaattct tatctgcaat ttttggtatt ctgtcatcaa accttctatt
    20641 aaacagtgta aattgtgtat tgaaaatagc actagcaaaa aatggtatta aaaagaatag
    20701 tattgcattt gctgcagcgt gccctaccac aaaaataagc aatactaaaa aagcaataac
    20761 tgtctgcaga atacttaata tacttccatt taaaacataa cgattcgcaa gcaactcata
    20821 ttgatctgtt gcatcgatat ctacctcttt atctacaagt tgcttatact tcaatgcacg
    20881 tctttgattc atcaaaacaa tggcttctac caatatgata ataatcgtcg caacaatcga
    20941 tattacaacg acattgttat atgtcgcaaa cgtcaagttc tcaataccaa atccatcaaa
    21001 attacctaaa attccaccta tgataccccc tacaagtcca cccagtaaaa gatatccgat
    21061 atatcttagt attttcatgc ctcatcctcc tcaataataa aaacagtttc caccgtttca
    21121 ttgaaaatgc gagcaatttt tattgccgtt aatactgatg gcataaaatt gtttcgctca
    21181 attagcgata tggtttgtct tgaaacgccc gcttgtttag caagttgcgt ttggtttaag
    21241 ccatctcgtg ctcgtaattc tttcaatcga ttacgcacat cgcatcaact ccttaattac
    21301 actgtaaatg atatgcgtct ttttgacaac tatatttgtc aaatctacac caaaaaatat
    21361 gattatccac ctatgtatga cattttgaaa caaacacctc aacgcctaca agtcataatt
    21421 gtttactttc gttacacctt cctgcataat taacagcatt ctaattttag tatgatgcac
    21481 gcattttcac taaatcaaac cattcaaagg agactattat ggcatttaca ttatctgcaa
    21541 ttcaacaagc acatcaacaa tttactggtg ttgactttcc aaaactattc aaagctttta
    21601 aagatatggg gatgacttac aatatcgtca acattcaaga tggcactgca acatacgtac
    21661 atcaatcaga agatgatatc gttacgtcat ctgtaaaaag taatcatcct gttgctcaaa
    21721 aatcaaacaa aacaatagtt caagacgtct taactagaca tcaacaaggg caaacagatt
    21781 ttgaaacatt ttgtgatgaa atggctgaag ctggcattta taaatggcat atcgatattc
    21841 aagcgggcac ttgtacttat atcgacttgc aagaccaagc tgttatttca gaattaatcc
    21901 ctcaataaac tatatttata gcaacatttt aattatttca taaaatttta ttgataatca
    21961 ttatcgttcg gtataaagta aatactatat actacttatg agtgaggttg attatcatga
    22021 taactaacac ttttatttta ggcatcacag gcccaacaag tcttgtcgtc attagcatta
    22081 tcgctttaat tatttttggt ccgaaaaaat taccacaatt tggccgtgcc atcggttcta
    22141 ctttaaaaga atttaaatct gcaacagaag atttagataa agagtctcac gatacaccca
    22201 gtaaggaatc gaaacaacag cgagagcaat agcactgacc acaccttact ggttcacttt
    22261 agcgaactac gccatcggtt agtaaaaatt ttattgtcgt tcgtcattac ggtcatcgtc
    22321 gtatatgttt catcattttg gtggatgaca ccattcataa cgtatattac ccgtgcacat
    22381 gtgtccttac atgcattttc attcacagaa atgatacaaa tatacgtgat gattatattt
    22441 ttcatcgcgt tttgtttcat ttcaccagtc atgttttatc aattgtgggc attcattgca
    22501 cctggattac ataataatga acgtcaattt atttataaat atagtttttt cagcgtgtta
    22561 ttattctgtg ctggcgtcgc ttttgcattt tatgttggct ttccaatgat cattcaattc
    22621 gcgttaaaat tatcattaac tttaaacatt tcgcctgtca ttggctttaa agcatatttg
    22681 attgaactca tacgttggtt attcacattt ggtcttctat ttcaattacc aatattattt
    22741 atgggtcttg caaaattcgg tcttatagat accacatcat tgaaacatta tcgtaaatat
    22801 atttatttcg cttgctttgt attagcaagt atcattgcac cacccgattt aacattaaat
    22861 atattactca cattgccact catattgctc tttgagttca gtatgttcat cgttaagttc
    22921 acatgccgag gcaaaccgcc aacacattaa ccttatacga atcaatgctg tgattatcgt
    22981 tatactgcaa taccgaatat ttccaatata gcaacaaaaa aacaggcact cggcctgttt
    23041 tttatttttt aaatttaaag attaagatag caactaccat aatataagca ccttgtgcta
    23101 tcaatggttc ataacttgga taaaagccca accagttaat cgttgggaat ccttcaataa
    23161 catgtcttgg catcgcacct aataattgta acttttgaat acttacgcca agcattttga
    23221 atcccataat aaaaataaag atcgacaaca ctctaaatat atagaaaata ggtattaatt
    23281 taactataaa tctaaataat aatgcaaaga tgattaaaat aacgatagct aaagcaatac
    23341 caataataaa atctttggtc gctagctcac ctatcatccc catatagaaa atgataacct
    23401 cgacaccttc acgcaacaca gatattaaac caatcgtcgc taacaatacc aaattaccat
    23461 tactaatcgc attagcatac atatttttaa tcatgtcatt ccaacgtttt gcatttgaac
    23521 gtttgtgcat ccaaacacca acgataaaca ttaatatgac cgcaacgata cctaatcccg
    23581 cttccatact ttcacgaaga atgccactat tccctaaagt ttctacaaac gtaattgcta
    23641 agataatact cagtacaagt ccggcaattg caccaccaat cacacttgca gtccctttct
    23701 tatcttttac attacgcgtc atggtagtca atgtcattac aattaacaac acttctagcc
    23761 cttcacgtaa aaagataatc atcacatcga cgaagctata actatggcca acaacctctt
    23821 taatttggtt atttaaatct actaaaccat ctttcacatg tgctttatta tgttcgtcta
    23881 atacactttg ataatatggt attttatctt caattttcgt atacaaagca ccgtctttag
    23941 tttgaatttg accttcaaca tacggccaag tttctataaa atgtgtaagc gcagcatcag
    24001 catccgacaa ttgattgtcg tcgatagctt taatcgcctt ctctaacgca tcatttaatt
    24061 gtgatacatg gtattgatca tttgcagacg tattactttt tttatcgaca tgatcaatat
    24121 ttgatttaaa agttgtccaa gcatgtgaca cttttgccgt atctaatggt gacttatgaa
    24181 ttgcaattct aagttgtaat aatgcgactt caatttgtcc atattgattt gcgtcataat
    24241 tgcgaatcac tgtttcatta cttgtccaaa tctgattcaa actattgttc aaagattcta
    24301 attccgcttt atttttatct ttaatcgctt ttgtcatcgc agcatcttta gcatcgactt
    24361 gctgttgcaa tagtttaatt ttagaacccg catctttact agccaatttc tcttcataag
    24421 caattaatga cttcgttaat tgcgaaagtg tatctttttg attatcattc gcttttgcat
    24481 cttcaagctt tctcacatct gatttgacag cattactttc actattatct tcaagcgata
    24541 atttcttaac tgcacttacc acttgctcaa ttgctttctg cttattgtca ttcgatatcg
    24601 aattattaga aagtgcagat ttcgcatccg ttatcacact atatacatca ctaatacttt
    24661 gttgttctgc tgcctgactt ttcagtaacc caaagctaca caccatagca gcagttatta
    24721 gcattgctac aaatttagtc aaataatgtt tcaccaaggt atcctccctt actaacacct
    24781 ggtaatacta aaaatgaagc agaacctcta tgtgtaatat attcatttaa tttatcatta
    24841 ctacctaaat tattttgtat atcgataaat tgttttgtcg ctttttgaaa agcaataaaa
    24901 agtaagcctg tttcgaagtt acctgtgcgg tcatccgtac catccacata attaaaggct
    24961 ctacgtaaaa ttgacgtatt tgcttctttc gctagcctcg tatgggcatc tttatcaata
    25021 atatactcgc catgactatc tttcgctttt aagtcaattt catcaaactc tttcccacct
    25081 gttaacggtg caccactatg tcgtttccga ccaaatgtag cctcttgttc ttccagcgca
    25141 gtacgatccc acgtttcaat gtgtatttga atacgtctga caacgcaata agttccatgt
    25201 ttcgcccatc cgtcatcaat aaacacataa tctttaagtt gattattctt cctaggatta
    25261 attgttccat ctttaaatgc cattaaattt ctaggtgttt ccttaccttt agcagagata
    25321 aaaccagatt gcgcccaacg taccttaaca atatctcgaa atggacgaac taaattatga
    25381 accgcatgaa aggatacttg cgaatcattt gagcatgctt gaatcataat atcaccatcg
    25441 ctgtaatcgt ctattaactg atcattcgga aaatgcggta aatctttaaa ggcatcggga
    25501 attttactag ataacccaat tttcttcatc aaagacttac taatcccaaa ggtaatcgtt
    25561 aacttgcttg cacctaatcc tatcgattca ccggtatcta ctggtggcat taaaggattg
    25621 ttactcggct tgccaactgt atcaccatcc atcatacgat cagccataac cgtccacttt
    25681 ttaaacattg ccttaattgc atctctatct tttgacttca aatctaacgc aacgaaattg
    25741 catgtttttt gcgtgggtgt ggtaatgcct ggttgcactt taccataaaa ttcatacgca
    25801 tctttttccg gatcttctgg tgtattgaac attgatttga aagaccacat gctaccaaca
    25861 ccacttgcgc caattgcaac accggcaccg ccaataccta acattttcaa aaatgtacgt
    25921 cttgaaaatt gcgtactatc gttaacttgt tcataatttg tcattcagta atcacagcca
    25981 ttttacttag tggctctcct aatgcattaa cagcatccgc aaatgcttta cggtctttct
    26041 tcgatacttt ttcaaatgac tcataaccgc cgttgttatc tttatattta tccaataatt
    26101 gattcacttt atcgaagttc atttggatat catcacttaa ttttttatct tttttctcta
    26161 aaataggttt aaataagtca taaatttttt gtgcgccttc aacgttcgct ttaaaatcat
    26221 ataaatctgt atgtgaataa atttcttctt cacctgtgat tttagaagtt gcaacttcat
    26281 ttaataggtc aacagaacct tgtaacatta attttggtat gatatctaat gtatcagctt
    26341 tggcatgcaa ttcttttgca tctttcaata attgttgtgc atctttttta gtcacatcat
    26401 caattttctt atcttcgtat aatgcttttt caatcttatg atatcctgac cattcttttt
    26461 cctttttctc ttctttcata tctgcaagac gtgcatcaat tttaggatct aaatctccaa
    26521 atgcttctgc aactggttca gagcgttcat aatacatacg aacttttgga tataacgctt
    26581 ttgcttgggc catatcatta ttttcaatcg ctttaacaaa tttttctgta ccttttaaaa
    26641 attcatttaa ctgttcatca gtatatttat catatgcttt tgttgcttgt tttaattctg
    26701 ctttaacacc atcatctttt ttcgatgtct ttgaatcatc cttcttacta tcgtcgttcc
    26761 cacatgcagc aattaataac gttgatgcta ataatagcgt tgttaacttt ttcattttga
    26821 cacctcatta tagaaattct attatcccca tagatgtgtt cttataaata ttattttcgc
    26881 attccctaca cgctaaaata atacattaca cttaaaacgg ctgttttaaa gcatcctccc
    26941 ataaacatca tctagttgat aataattatc aaaatcatta tattgttata aaataatact
    27001 gtcaacaacc ttttattttt aaaaattaaa taaaagagcg ctgccatttt aaatgtcatc
    27061 actcttttg
//
LOCUS       DANJFZ010000028        24050 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.028, whole genome shotgun sequence.
ACCESSION   DANJFZ010000028 DANJFZ010000000
VERSION     DANJFZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.028"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(38..157)
                     /locus_tag="PWN45_001019"
     CDS             complement(38..157)
                     /locus_tag="PWN45_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622798.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(237..377)
                     /locus_tag="PWN45_001020"
     CDS             complement(237..377)
                     /locus_tag="PWN45_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622799.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(381..980)
                     /locus_tag="PWN45_001021"
     CDS             complement(381..980)
                     /locus_tag="PWN45_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDL0622800.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            1160..2797
                     /locus_tag="PWN45_001022"
     CDS             1160..2797
                     /locus_tag="PWN45_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0622801.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            3405..4757
                     /locus_tag="PWN45_001023"
     CDS             3405..4757
                     /locus_tag="PWN45_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDL0622802.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(4819..6006)
                     /locus_tag="PWN45_001024"
     CDS             complement(4819..6006)
                     /locus_tag="PWN45_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0622803.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(6402..7178)
                     /gene="fetB"
                     /locus_tag="PWN45_001025"
     CDS             complement(6402..7178)
                     /gene="fetB"
                     /locus_tag="PWN45_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDL0622804.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(7171..7833)
                     /locus_tag="PWN45_001026"
     CDS             complement(7171..7833)
                     /locus_tag="PWN45_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0622805.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(8081..9157)
                     /locus_tag="PWN45_001027"
     CDS             complement(8081..9157)
                     /locus_tag="PWN45_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0622806.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(9512..9778)
                     /locus_tag="PWN45_001028"
     CDS             complement(9512..9778)
                     /locus_tag="PWN45_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0622807.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(9778..10035)
                     /locus_tag="PWN45_001029"
     CDS             complement(9778..10035)
                     /locus_tag="PWN45_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0622808.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            complement(10141..10335)
                     /locus_tag="PWN45_001030"
     CDS             complement(10141..10335)
                     /locus_tag="PWN45_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622809.1"
                     /translation="MMLFKNLNFVIFFEDMSFFKRIPHKILKIFKPENDKAVGNVVCS
                     TADFFLSIIGGMLEEKNKSL"
     gene            10394..10849
                     /locus_tag="PWN45_001031"
     CDS             10394..10849
                     /locus_tag="PWN45_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDL0622810.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(11017..12594)
                     /locus_tag="PWN45_001032"
     CDS             complement(11017..12594)
                     /locus_tag="PWN45_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDL0622811.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            12789..13538
                     /locus_tag="PWN45_001033"
     CDS             12789..13538
                     /locus_tag="PWN45_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622812.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(13762..14955)
                     /locus_tag="PWN45_001034"
     CDS             complement(13762..14955)
                     /locus_tag="PWN45_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0622813.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(14967..15716)
                     /locus_tag="PWN45_001035"
     CDS             complement(14967..15716)
                     /locus_tag="PWN45_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622814.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(15709..16524)
                     /locus_tag="PWN45_001036"
     CDS             complement(15709..16524)
                     /locus_tag="PWN45_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622815.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(16521..17390)
                     /locus_tag="PWN45_001037"
     CDS             complement(16521..17390)
                     /locus_tag="PWN45_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622816.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(17387..18322)
                     /locus_tag="PWN45_001038"
     CDS             complement(17387..18322)
                     /locus_tag="PWN45_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622817.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(18335..19933)
                     /gene="cntA"
                     /locus_tag="PWN45_001039"
     CDS             complement(18335..19933)
                     /gene="cntA"
                     /locus_tag="PWN45_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDL0622818.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(20076..21377)
                     /gene="cntM"
                     /locus_tag="PWN45_001040"
     CDS             complement(20076..21377)
                     /gene="cntM"
                     /locus_tag="PWN45_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDL0622819.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(21370..22188)
                     /gene="cntL"
                     /locus_tag="PWN45_001041"
     CDS             complement(21370..22188)
                     /gene="cntL"
                     /locus_tag="PWN45_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDL0622820.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(22199..23020)
                     /gene="cntK"
                     /locus_tag="PWN45_001042"
     CDS             complement(22199..23020)
                     /gene="cntK"
                     /locus_tag="PWN45_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDL0622821.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
BASE COUNT         7663 a         4632 c         3560 g         8195 t
ORIGIN      
        1 ctaaatagat aactcatatt ttttaaaagc gaatttatta tcgctcaatt ggttgtttta
       61 tagcaaaaaa gtgtcgaaaa tgtgaaatat tattacaaaa gtcgaatttt tgtaataatg
      121 cacacgcatc cattgttaca gacggacaac cgcccatccg ccactcacaa agtcatcctt
      181 atattttaat atcttcatca ttcattgtag acacccttgc cttttgctaa cattttctac
      241 tcccaagcct gtaacggaat tccaacgtca aagtgttgcc catgacagac acacctaaaa
      301 aaacaaccaa cagcatgagc cattggttgt ttcgtgtcat cttgtatgtt gttgtataac
      361 cggtatgctt tactcattaa ttacttatca tgcatccctt taccttctag tatttggtta
      421 atatactttt caacacgctt ttgtcttgtt tcactgcgtt tagcttgtcc aatatgatat
      481 atatattgat gttgtctgcc tggcgttaat ttataaaatg aagactctaa ctgtggcatt
      541 tcttcgaact tagcttctaa ctctttagga ataacatatt cctctgtttt cttcatttca
      601 acttttttac cagctttttc agctttaatt gcttcggcta gataatattt aatttcttcg
      661 gtacgtgctt gaatctctgt taaattttca aatcgtaact gacgagctgc ttgcaccttt
      721 tcagtctgtt gaatgagtgt atgatattta tcctccaaaa tggcaccttt atgaaataat
      781 agtgcaacat aatttttgaa gccatggata agtactacat ttttattatt caacgtgtaa
      841 caaggatgca tccatttata cgcttcctct aattctgttt cattaaagat taacgctctc
      901 aaatatttat attcttcttg ccattgactc tctttattta aaaatgcttc aacttgttca
      961 tttgattgtt tctcactcat agtcatcact tccattttgt ttttcaatac acattgtatt
     1021 atatatccta attttataaa aaactaattt tacaaatgct tttgcgttct tacaaaaaat
     1081 gcatttgact attattataa taagcgtata attgtcgcat attatttttt gtatttttgg
     1141 caataacgaa ggagtattta tgaataaaga caagcaattg cacaacgaca aaatcaatct
     1201 atcccaatta gtcttattag ggttaggctc tttaatagga tctggttggc tatttggtgc
     1261 gtgggaagca tcatcaatag ctggaccagc agcaatcata tcatgggttc ttggattcct
     1321 agtcattgga accattgcct ataactacat tgaaatcggc acaatgtttc ctcaatcagg
     1381 tggcatgagt aactatgccc agtatacaca tggctcatta ttaggcttta ttgctgcttg
     1441 ggcgaattgg gtgtctttgg tgacaataat acctatcgaa gctgtgtcag ctgttcaata
     1501 tatgagttct tggccgtggc attgggcgaa accaatgaga tatttaatgg aaaatggctc
     1561 tattagcaca tacggattgc tagctgtata tctcatcatt gttatttttt cattattaaa
     1621 ctattggtcc gtaaaacttt taacatcatt tacgagttta atttctgtat ttaaattagg
     1681 cgtacccatg ttaaccatca tcatgttgat gctatcagga ttcgacactt caaattacgg
     1741 ccattcggca agcacattta tgccttacgg aagtgcaccg atttttgctg caacaacagc
     1801 atcagggatt attttttcat tcaattcatt ccagacaatt attaatatgg gttcagaaat
     1861 taaaaatcct gaaaaaaata tcgcaagagg catcgctatc tcactgtcaa tcagtgcagt
     1921 gttgtacatc attttacaaa gtacgtttat cacttctatg cctcaatcaa tgttacaaca
     1981 tagtggatgg aatggcatca acttcaattc accatttgct gatttagcta tcttattagg
     2041 aattaattgg ctcgcaattt tactatacat tgaagctttt gtatcaccat tcggtactgg
     2101 cgtgtcattt gtcgccgtta caggtcgagt tttacgagca atggagaaaa atggacatat
     2161 ccctaaattt cttgggaaga tgaatgaaaa atatcatatc ccacgtgtag caatcatctt
     2221 taatgccatc attagtatga ttatggttac attatttaga gattggggta cgctagcagc
     2281 agttatttct actgcaactt tagtagccta tttaactggc ccaacgacag tgattgcatt
     2341 aagaaaaatg ggaccaacaa tgactcgtcc atttagagca aaaattttaa aagtaatggc
     2401 accattatca tttgtattag cttcattagc tatatattgg gcaatgtggc caacaacggc
     2461 tgaagttatt ttaatcatta tacttggatt accaatctac ttcttctatg aatatcgtat
     2521 gaattggcgt aatacaaaga aacaaattgg tggtagctta tggattattg tatatttaat
     2581 cgtgctatca atactgtcat ttataggaag caaagaattt aaaggcttaa atatgattca
     2641 ctatccattt gactttatcg ttattattat tgtggcactt atcttctatt acatcggtac
     2701 aacgagttca tttgaaagcg tctatttccg tcgcgcaaca cgaatcaata cgaagatgcg
     2761 tgagtcacta aataatgaat caaagtcatc tcattaaata ttttaaaata cagtgccata
     2821 tatgatttca acacacacac attaaccaac cattgatttc aacatcttgg ttggtttttt
     2881 attttgaaaa tcggttataa ataactaaca taacaagatg atgatcaggc tgggacataa
     2941 atcaatgttc tatgctctac gaagttatat tggcagtagt tgactgaacg aaaatgcgct
     3001 tgtaacaagc ttttttcgat tctagtcagg ggccccaaca cagagaattt cgaaaagaaa
     3061 ttctacaggc aatgcaagtt ggggtgggac gacgataaag aaatactttt tctatagaaa
     3121 ttagtatttc ttatgcatga gttttactca tgtattcata tttttaagta cacattagct
     3181 gtggctaatg tataagaacc actacataat aaatcatttg tggctcttta tcatttctgt
     3241 cccactcccg tagaagtaca tcatataatg ctgaaaatgg tttgagttaa aacagatatc
     3301 aagctcgtct gattcagtca caaaattgtc ttgttatact tgtcacctat catctataga
     3361 ccgtggtatg attaaattgg ggatgataaa ggaggttaat aaatatgaag attaatacta
     3421 caggtggtca aattcatggt attacacaag atggtttaga tatcttctta ggcattcctt
     3481 atgcagaacc accagttcat gacaatcgct ttaaacattc tacgttaaaa acacaatggt
     3541 cagagccaat tgatgcaact gaaatacaac ccatcccacc gcaaccagac aacaaattag
     3601 aagatttttt ctcctcacaa tctacaactt ttactgaaca tgaagactgt ttatatctaa
     3661 atatttggaa acaacataat gatcagacga agaaacctgt catcatttat ttttatggtg
     3721 gtagttttga aaatggtcat ggtacagccg aactctatca accggcacat ttagtacaaa
     3781 ataacgacat tatcgttatt acatgcaatt atcgtttagg cgcattagga tatttagact
     3841 ggtcatattt taataaagat tttcattcca ataatggcct ttcagatcaa atcaatgtca
     3901 taaaatgggt gcatcaattt attgaatcct tcggtggcga cgctaataac attactttaa
     3961 tgggtcagtc tgcaggcagt atgagcattt tgactttact taaaatacct gacattgagc
     4021 catacttcca taaagtcgtt ctactaagtg gcgcactacg attagacacc cttgagagtg
     4081 cacgcaataa agcacaacat ttccaaaaaa tgatgctcga ttatttagat acagatgatg
     4141 ttacatcatt atcgacaaat gatattctta tgctgatggc gaagctaaaa caatctcgag
     4201 gaccttctaa agggcttgat ttaatatatg cgcctattaa aacagattat atacaaaata
     4261 attatccaac aacgaaacca atttttgcat gttatacaaa agatgaaggc gatatttata
     4321 ttactagtga acagaaaaaa ttatcgccgc aacgctttat cgacattatg gaattaaatg
     4381 atattccttt aaaatacgaa gatgttcaga cggcgaagca acaatcttta gcgattacac
     4441 attgttattt caaacagccg atgaagcaat ttttacaaca actcaatata caagattcaa
     4501 acgcacaact atggcttgct gaatttgcat ggcatgatac ttctagcgca cactatcgca
     4561 gtgcttacca tattctagat atggtatttt ggttcggaaa cttacaaata ttagcagcac
     4621 atcaatatcc aacaactgct caccttaaat ttttaagtcg acaaatgcaa aatgatttag
     4681 ctaattttgc taaaagcggt aaaatgcctt ggccgatgta tcataatgaa cgtcgctatt
     4741 atcgcacata tcaataacgt tatcgcatct aaaaaagcac acctgaattt gatagcaggc
     4801 gtgcttttca tatgagaatt atgatgattt aaatgtttta gcataatgat tatttttcaa
     4861 tgtgaaaaca acgattaatc ctaatgcgcc aatgatggca ctcgtatatg tgatagcttg
     4921 aacagaaaca tgtgtcatga ccaagcctcc aataatgcct ccaacaccaa taccagcgtt
     4981 taaactagac atgttccaac tcattacttg gcttgtgtcg ccttcaacat gttgaatcac
     5041 accgctttgc actgctggat tagtactcca ttgcatgata ttccaaataa atagtcctgc
     5101 taacaataga cctgaaccag gtaagattaa attcataagt aacatcatga cgataaaaat
     5161 agaaacagaa atcattaacc aacgcttact tgtaatttta tcggagaata taccacctaa
     5221 tgatgttcca ataacgccag cgattccatt tactagaagt gctaatgaaa cgaatgacat
     5281 atcatgacca ttagataaaa taagtggatt tataaagacg aatgtcactg agtttgcaat
     5341 caatactaaa aacgtaataa ttaaatattt tgctacttca gcaggtctta atattttcga
     5401 agtaacatga ttttcatgag atggtgtctc atgattcaca gggcctcgtt gtatttcctg
     5461 atccttcggt aaatagatca tcatcaagaa gccaacaata atactcacaa taattaagaa
     5521 tagaaatgta tagcgccaac ctactaaatc gccgataacc gttccaattg gtacaccaaa
     5581 aacattagca ccactaaacc ctgtatagac aagtccaatc attttaccac gatttttagg
     5641 tgctgataac atcgctgtaa tagctaatac ttttacgata attagtgctg ctgctgcaga
     5701 tgagataatt ctaccaacta ctaatattga aaaatttggc gctacagcaa taatgccatt
     5761 accaatgata aatataagta atgtccataa taatacaggc cttgatgaaa aacggttcgt
     5821 taatttaacc agaataggtc cacatatcgc aaatgttaaa gcgtacattg tcactaattg
     5881 accaacgaca gcttctgata catgtaagtc ctgactcatc aagttcataa ttcctgcaac
     5941 catcatttca accattccta cgataaaaat acttaaaatg aatgtaaata gtcgcatgat
     6001 tgacaattta aaactccttt caaacaaaat ttccgatatt catttccctt agaataacga
     6061 tttattaaaa aatgtgtgca acacaatttt atatataacc gttctattat ataatatttt
     6121 gctttaattt tctgtgctta attggaaaat tattgaaatt gtttttcagt atttgaaaaa
     6181 ttaacacaag tatgtcattt aataattatg tataattact tcagtttcaa caataactcc
     6241 aaaaaagcac gcacgcttta tatgtcagaa cagataactg actacaaagt atgcgtgcct
     6301 ttatgaatat tttcgctgcg ctaatgaata gcgatactat atgtgcgcgt cacttcattt
     6361 ccaattaaaa ctaatgccta aatctgatgc agtaaaatct atcatgattc actcttaaca
     6421 tcagtatttc gtgctactaa ttgatgtctt gcattgaaaa attgaccata gcttaaatat
     6481 gtcgcaatca aagcagacat aatggtcgca gttgtatgaa taaacacgac taacaattga
     6541 aatttaatcg cttgtaaagg tggtacgcca ccaataatta agcctgtcat cataccagga
     6601 atcgacacaa gcccatatgt tttaaccgaa tcaatagttg gcactatagc taaacgaata
     6661 ctttcacgta ttgcaccttt agaagccaat ttaggtgtag ctgcaagtga taatttagat
     6721 tcaatattag taccatcttg tacgaatgca cgatctaaat tctggtaagc taaattaatt
     6781 gcaatcaagc cattatttgc aagcatgccg ccgataggta taacttcatt tgcggtaaat
     6841 tgaatggccc ctgtcgcaat agtacctgca agcggtaatg ccgttccaat gaagatagct
     6901 agaaatgata tccaaaacac atgatgcatc actggtgatg ctcgactaat tgtattccat
     6961 gatgcattaa taataatgac caatacacat aaaataagca gccatttatc gtttatttta
     7021 aaaatataat gcagcaaaaa tcccaaaatg attaattgca caactgctcg taatgtcgca
     7081 acaattaaat ctttaataat atgtaaacct tctttatatg aaatgataat cggaatgact
     7141 aaaagcaatg ccgtcaatcc aagtgccgta ttactcatgt tgattcaact cctctgtatt
     7201 agaaatttga ccatcaacaa ttgttatacg cttttgaaag tgtcgcatac tttggtcatc
     7261 gctgtgggta atccacataa ttgccacgcc ttgatctgct aatttaaaaa tgatattttc
     7321 tatcttttct ttattattaa cgtctaatgc actggtcgat tcatctaata aaagaatatc
     7381 cggtgtatac atcagttggc gcgctatagc aattctttgc cgctcaccac ccgacatatt
     7441 ttccacttcc gaacttaatt gataatgtcc caaaccgaca tctttaatta attgctttgc
     7501 acgttttcta tcaaatttat cattacgtgc aagtgatggg aatatcatgt tatcttcaat
     7561 cgtttcacca aacaagtcac tttgctgcat caaataactg attcgttgac gcaattcttc
     7621 cgggtcataa tcattatagg gtttaccttt aaaataaagt tctccactag ttggactaaa
     7681 caaattacat atttgctttt gaaatgtact tttaccacta cctgatggac ctataatggc
     7741 aatactctcg cctttatcta cttttagact gatatgatct agtatgattc tatcgctcgc
     7801 tttatacact aaatctttaa tttctaacaa catttgcacc gctcctcatg taatatatca
     7861 ggtatattat tcccgttttt ttggttttta gtgcaattac atttaagaaa aaatcatttt
     7921 cttgtaatat caaaatttag gcataattga ttttcagcat ttaaattaaa aacaaaaaat
     7981 cctattaaca tggatactgt ctaatgacta agaattaaaa ttagacaatt gtttaacaac
     8041 ctaccatatt aaaaggattc tttattattt atggatttga ttaccacatg atatttttat
     8101 aactttcatc attcaatgaa cggacaattt cagtaacaag acggatagaa ttttcataat
     8161 catctacatt gagcactgaa acattagaat gcatgtatcg cagcgtaaca ccgattgtca
     8221 tcgttggaat accttcattt gcgacatgaa tacttcccgc atctgtacct ccacctggtg
     8281 tcgtatccca ttgtacttcg atgttatgtt ccttagctac atctttaata tgctttcgca
     8341 aaccttggtg agcaatactt gtagcatcca tcataatgac aactggacca ccgcctagtt
     8401 tactatcgct cgtttgacct gacatacctg gggtatcata agcaatacct acatcgacag
     8461 ctatcgccaa gtctggttta atcgtattcg ctgccacttt cgcaccacgc aaaccaactt
     8521 cttcttgcac tgtggcacca ctgtataagt taatgccaat attttcatct tttaaacgtt
     8581 ttaatacctc aatagctaat gcacagccat agcgattatc aaatgctttc gcagttaaat
     8641 atttatcatt tgcaagcact tcaaattcac tatatggcgt aaccatattg cctacttcaa
     8701 cgccagcttc ttcagcttct tccttgctac taacaccaat atctataaac atatttttga
     8761 tttccattgg ctttttacgt tcttcaggcg ttaagacatg tggcggttta gaaccgatga
     8821 tacctctaat ttctttgccc gaatctgttg taatcgttac tttttgagat agcatgactt
     8881 gattccacca tccaccaact ggcgtaaatg aaataaaacc atgtttatca atctttgtta
     8941 ccataaaacc aacttcatcc atatgaccag aaatcataat tgagtattga ccattctcag
     9001 catttttctt tccaaaaatg ccacccaagt tatcttcaat aatttgatca ctgacaggct
     9061 ctatatagtt acgcattgct tctttaactt gcatttcata accagcaatg ccgtttacat
     9121 ctgttaaatg tttaagtaat ttgactgact gattcatgtt attcatcctc ctgtatttca
     9181 acttaaaact attgtatcac taactttcag aaaaacgttc cctaccgctt tatcattttc
     9241 aggcttaaat atttttaaaa ttttgtgagg aatccgctta aaaacctcat atttctaaaa
     9301 tttaatattc cgaaaaagtc acaatttaaa attccttatt ataatacgtt attcttttat
     9361 tgttactaat ttcaatctgt ttagtcttgc gctattataa aattaaaaag tgtagagata
     9421 acaacgcatt aacgtcgcta ttttccctac acttaaatac tagtttcttt aattaactta
     9481 tcattaaagt tattcagata ttgaatattc attaatcata atgtgaccat gccgatagta
     9541 ttaatacttc tttatttcga tcatctacgg tatagacgac cctgtgctga tggttaattc
     9601 ttcttgaata tcgctctaaa tatttaggct ctaatttttc aaaagattgt gttattttat
     9661 acggatcatt ttttaaagtc tcaacaattt ctaaaaatga cttctttaaa taagaatgtt
     9721 ttattttctt taaatctgat ttcgctgaat ttttaatctt aaccgtgtaa ttgctcatta
     9781 aagattatcc caatcaatat catctatatt tgttgtacca ctattatctt tttctctttc
     9841 tcttactttg tccattgtac cagtagattc aagatatatt gtctcttgta tacttttcca
     9901 atcttctaaa cctataatca cagcattatt ttcggcatta ttgccactaa tataaattgg
     9961 ttcgtgatta ttattaacat tttttagtaa ttgataaaaa tcttttctcg cttctgtagg
    10021 gctagtgata atcatataaa ccctcctttg ttttctgtta cgctaattat agcgtacgca
    10081 cttgagtacg tcaatattac caacgagttc atcgtattct taatacctat atgctatcgt
    10141 tcacaaactt ttgttctttt cttccaacat accgcctata atgctcaaaa agaaatcagc
    10201 agtggaacac actacgttcc ctaccgcttt atcattttca ggcttaaata tttttaaaat
    10261 tttgtgagga atccgcttaa aaaaactcat atcttcaaaa aaaatgacaa aatttaaatt
    10321 tttgaataac atcatttttt agtaaatgtt ataatttctt tataatacaa atatcgaaag
    10381 aaggcgactg actttgaaaa aattatgttc attaattgta gtagcattag tttgtatcat
    10441 tgcattatca gcttgtggta aagaacaaac caaaacatat gaaggtgatg ttagtggtaa
    10501 acatgtacta acttctatca catataaaga tgataaagtt ttaaaacagt ctacaatcaa
    10561 cacaattaaa tatgacgatc taggtatgga taaagacgaa gccaaaaagt tattcgccaa
    10621 atctgaaagt attttcaaag accttaaagg cgtaaaatac aaagtagact ataaagataa
    10681 aaaagcaatt gaacacttag acatagatta cacagaagtt gacatgaaaa aattaaataa
    10741 acgtcttggt gtttcgacta aagaaaataa agatattagt tttgaaaaac ttgaaaagca
    10801 attaaagcac agaggtttaa aagaaaaaga taaaatggac gacaaatagt ttataactta
    10861 aaaatgccct cagataagac taaggttaca aaccttaatt catattctga gggctttaat
    10921 atttgaagtt cttgtgtgac cagcatccac tactaatata aaattatttg cagtaacgct
    10981 aaaatccgct gctttcaatt tcccgaaata attaagttaa ctaatgagtt ttaatttata
    11041 atcatgtatc gtttgtaact caccatcgac ttttcgatat acaatatgat cagcagtaat
    11101 ttctgtagga ctggatacgc caacagctgc tgcaatattg aataagcctt catgcaaact
    11161 tgttacatag tttgtgacac gatattgctt ttctccaaca atcaatgctt tttctttctt
    11221 cgcatctgtc gttgcaacac ctacaggaca cgtattcatg tgacattgtt gactcattat
    11281 acaaccgaca ctaatcatca tcccacgtgc gatatttaca aaatctgcac ctaaacctag
    11341 tgcaatcgca attttatctg gtgtcactaa cttaccagat gccgccaatt tcactttatc
    11401 tcgaatacca tatttttcta acatgccaga cacaataggt agagctgtaa atagcggtaa
    11461 gccaacacca tcttgtaatt cttggaatgt tgcaccagta ccaccttcac caccatcaat
    11521 cgtaataaag cttggatact tatctagttc caccatcgta cgtacaagtg tttcaatttc
    11581 tgaaactttg cttactacaa ttttgaatcc tactggtttt tgacctaatt gctgcaactg
    11641 atcgacgaaa cgaatcaaat cttcagcatt atgaataaat tcgtaacggt taggtgaatt
    11701 gattgtttta taaggttcaa catttcggat tttagcaatt tcttcgttta ccttttcagc
    11761 ttccatatga ccaccacgag tcttagcacc ttgtgccaac ttcagctcaa atgcgcgtac
    11821 gttagataac tgtgcaacct ctttaaataa accttcacta aaattacctt ctttatcacg
    11881 aacaccaaat aaaccgggac caatttggaa aatgatatcc ccattacctt ttaaatgata
    11941 ttctgataag ccaccttcac ctgtattcat ccaagtgccc gctttagcta gacctttaga
    12001 taaagctgta atggcatttt ttcctaaagc gccataactc ataccagatt gtcctacgat
    12061 acgttttaaa ataaatggat gttttaaatg ttcacctaat tttattgcat ggtcatcact
    12121 taagtaatac ggatcaatct ttgtcggcac acgatattct tcacgactaa ataaacgctc
    12181 attcgcgatt ttataaatga atgttgataa caatgttgta ttatctactg aaatctcatt
    12241 acgttgcatc ggaaacattg tgttctgtat gtaaaagccg tcttgataat ctttagtagt
    12301 accgaagctg gtcatacgag agttatattt tccagccaaa acgatatttt tataatcatt
    12361 acgtgaaaaa ggtttccctt cattatcccc agaaaataaa tactgacgta attccggtcc
    12421 cattttttct gaaatatatc taatacgtgc tagtaaagga taattcctta atacactatg
    12481 ttgtgattgt cttttatctt taattaacca aataagcccg ataacaataa ccgtaagcat
    12541 gaatcctaca acgataatgt taactataaa ttgcatgact gtaagaaacg tcattacaat
    12601 acctcccccc aaaatttcaa ttcaatattt atgatacacc ttacaaaaca aaacacaatg
    12661 gaagcgcttc attttataaa acaattttat gatatgtttt tcattttaaa ttttaatgta
    12721 taaaacatac aatacaaagt aatatgtgct aaagtatcta tataatacaa ctatttaaga
    12781 ggtatactat gtcaaataca aataaacatt acatagaaga agaatacgct accgaacaat
    12841 cgcgtttttt caaacgtgat attggattta ttttccttta catatttttg gttaacatct
    12901 tgccgatcat tatcgtcttt ttaacttttg ggttagtagc tgtattcact caacaagctc
    12961 cgtggggttt ctcttttcct atttcaatga acattagcta tttactcgca gttattattt
    13021 cactagctat ttttcgtctg atgcatgaag atgcgtttat tccaatgata cgttcgcaaa
    13081 tcgttgctag tgctaaatat aaatggcata ttcttatatc atttacacta agtgtcgtac
    13141 tctttttact tttaaatcat ttcaatattt tcgcactaaa tgtagattcc tttactgtct
    13201 ttgacattta cccgctcatt aaagggtatg gttccatcag tttaataact atattttcgt
    13261 taaccattat attaaatact ttgatttgtc aacttatttt ccgtcatatt ttaattcaag
    13321 aattaagcag attaatacct ttaaagatta caatagtatt gtccattatc cttgaaactc
    13381 tgttctacta tccatacatt aatacatggt gggaattcat tccagcattg atactagcag
    13441 gtagtgcgac ttacttatat ttaaaatcat accgtaattt tatggtgagc tatttctatc
    13501 aattagcagt tatactcgtt ttgcatattt tcatgtaaaa tttttcagag tattatccat
    13561 tatccataca catcaaaaag cacacttaaa agtcagtatc aaaactcact tttaagcgtg
    13621 ctttatttag atatactgtc gttctaaaaa cgagccatat agaatgtttc atgtcctagc
    13681 atgaagcaca tgaatcgcca tgctcaatat accttttaaa atttaaatat tcattaactt
    13741 aatatgcttt aattcacttc attaaagact actcgctgga cgtggtgttg gctttttcaa
    13801 gaaactcata atcattatta caatgactaa cataccacaa attagataca tccatttaaa
    13861 tcctactaaa tcggaaatag gtcccatcaa tgcgccacct aacgatatac ctaagtctgc
    13921 acaggcaata aataaaccta acaacatatt acgacctact tttggtaaga cgaagcttaa
    13981 gtatgatgtt aatgttgggt acaccattgc ttgcgtcatt cctattaata tcgcactacc
    14041 atagaaaata attgcaccta cttgcggacc aaatgccact acaaatgacg cgattactaa
    14101 taatgatagt acagatacca tatatttagg atgccacata ccatctgacg gaatgtattt
    14161 ccttaagtaa aatcttgccg caacaactgc gatggcctgt attgtcaaaa agattcccgc
    14221 attcgcgaat cctaaactca ctgtgtataa cggtacaaat gtactaactg caccaaatac
    14281 aatcgatgca acaatcatga taataccact gttcaacaac tctttatttt tgaaaaattg
    14341 cgcaaaaaca gttacagcgt taaacggcat tttttcaatc ttatctgacg tatcgggttc
    14401 ctgttcagca aaggtcacgc gatagccaaa gaatgttgtt gttaatgcga taaagataat
    14461 gacaattgca aatagtgaaa tattatttgc attccaaata cctacggcaa ctaatggtcc
    14521 gattaagttt ggaatcgttg aaaatagcga gtacaatgat acaccttcag aacgatgttc
    14581 ctctggtaat gcatcaataa tacctagctg taaagacata gaaaagaatg ccgtacacac
    14641 accttgcata acacgtgcta cgaaataacc ttctaagcca gtaaaaccat aaataattaa
    14701 agctatggca ttgataatca aaataattct taatacttta atgggaccga cacgagcaat
    14761 aatttgtcct gcccatggtc gaaataccat tgctgttaac atgtatgccc ccataacgat
    14821 accgataacc gtattcgttg cgcctaaatc atgccctcgt aaaggtataa acacgtttaa
    14881 gattgcattg gcactaaaga acatcaatgt taatatatat aatctcaaaa agggccaagc
    14941 cattgcacct ttcattatct cactccttag aatgatagtt gtttttctat taattctctt
    15001 gtataagcat tgtcactttt atgcaatgcg cttgtcggaa tttgttcttc tatttttccg
    15061 tttttaaaaa taattaattg atcacataaa tacgtggcag cttgaatatc atgtgtgata
    15121 aaaatataac tcaactgacg cgtttcacgt aaatgaatca ataaatctaa tatttgtgtt
    15181 tgaattgaca tgtcgagtga actaatggct tcatcaaaca aaatatattt agggttaata
    15241 catattgcac gcgcaatcgc aacacgttgc gcctctccac ctgataacat attaggatat
    15301 ttatccatgt atgccttaga tagaccgact tcttccaaca atgtaattgc ttggacttcc
    15361 ataacttctt taggttgtcc atcacattga cacatcactt caaataagat ttctctaaca
    15421 gtctgaaatg gatgtaatga tgacgtataa tcttgaaata cagcaccaat ttgatgacgt
    15481 ctcactttct tcttatgcat cggttgatca tttaaggtta cacaaccttt atccggtttc
    15541 tcaataccta atatcatacg actcaacgtc gatttaccgc taccactttc tccgataatc
    15601 gcaatcgtcg caccgattgg acactcaaat gacacacctt tcacgatagg tgttcgacga
    15661 cgcttaaaaa catgtgcgct ttgatatgac ttttcaacat ctttaatttt aatcatgtac
    15721 atcacccctc atcacatgtt taaaatgatc attaatcttc ttcttcgttg ataataaata
    15781 cttcgtataa acatgttctg gatgatgcaa gactgattca cgtgtccctt gttcaataag
    15841 ctgaccattt ttcatcacaa caacacggtc tgcaatcttg ttaataaccg ttaaatcatg
    15901 tgaaatgaaa atcatcgcac agtcaaagtg ttttttaata tctataaatg cttccagtac
    15961 atcatattgt gtaattgtat ctaaagccgt tgtcggctca tcagcaatga ttaactttgg
    16021 tttcaaagct aacgctaaag caatcatcaa tcgctgtaac attcctcctg ataacatgta
    16081 agggtatgat tttaatatac gtttaggatc tttcaaactt aaataatcca tatattcaat
    16141 caatgtcttt tcaatttctt gtgtagacat tgacgtatgt actttcatag tctcaaacat
    16201 ttgtttaccg acagtagttg atgggtcaaa ggcacgacta ccttgttgca tgaccatcgc
    16261 aatgtcttta ccacggtact ttttcaattg cgattcagat aatgacaaca ttgatgtacc
    16321 atcaaagata atttcacctg tcaccccgag tcgttcggga ttcaaaccaa taatcgattt
    16381 acaagtgatt gatttaccac taccactttc tccaataacg cctaaagttt cacccttagt
    16441 taatgtaaaa ttcacatcac tcacgagtgg ttgatctgtc caggtatctg taatcgtcaa
    16501 atgtttaact gttaacaatg tcatgattgc accactcctt ttttcacaga acgaagttta
    16561 tctttagaag agatgcgggg atcaatagca atttgtaaag catcggataa gaagttaaat
    16621 gccatcacta taatcactat ggcaatacct ggcgcaaaca tcatttcagg atgtgtaaac
    16681 atcacttttc tagcttcgtt aagcatcatg ccccactctg cagtaggcgc tttgacacct
    16741 aatcctaaaa atgaaaagcc agatatttgc aagatcattg aacacatcga gctactagag
    16801 atgatagcaa tatctgctaa tgttaatggc ataatatgtt tgtgaataat tttcatatca
    16861 ttcataccga ttgttttagc aaatcttaca tggtcagaag cagtgtactg cataacactt
    16921 gtacgtataa cacgacagaa ccatgcccaa cgcgtcaaaa taaatgccat gataatattt
    16981 tcggcaccca ttccaaacaa tgcaattaat gctaacgtta caacataact tgggaatgcc
    17041 aacataacat cacacgcacg catgattaag gcgtcaacaa acccttggaa atatcctgat
    17101 aagaatccta aaatagatcc aataagtaca gaaacaaata gcgcaacaaa gacatataac
    17161 aaacttggtc taatcgcata aattaaccta gttaaaatat ctctacctaa atggtcagta
    17221 cctagtagat gttgaaaact catgccagca aatttgtttg ctgtatcgat atggttagga
    17281 tcataaaatg tcacaagtgg tgctgctaat cctaaaaaga catataatac aataatgcct
    17341 aaagcaatta ctgcaccttt atcttgtaat aatcgtttta aaattatcat cgtgcgccct
    17401 cccttaatct tggatttaat agcgcattaa tgatatctgc taatgtatta aatacaataa
    17461 ataataccgc tacaattaat acatatgctt gaatgactgg aaaatcgtgt tcaagtattg
    17521 cttttaaact taattgacct agtccaggcc atgcaaagat atactcgata acaactagtc
    17581 cacccattat cattggtata gacatacaaa agattgatac cgcaacttgt aaagcattac
    17641 gcaacacatg caacattaat gtgatagatt tcacaccgct tgctcttaaa taaagtacat
    17701 aatcttcatt taattgttcc accatcgagc gtctaacatt tctaaagtaa ataccagcat
    17761 aggcaatcgt aataacgatc actggcaata tgtaactttc tggacctgtt aatccagaag
    17821 tcggcaatat gtttaacttc actgaaacgt aaataataag tattgaagct atccaatatg
    17881 atggtaatgc agttagaaag aaagccactg aacgtatcgc acgatcagtg aactttcctc
    17941 tttttaatgc actaactaca cctaaaataa ttgatgtaat catcaccata acacttgaaa
    18001 ttattgttaa tttcaatgta ttcataaatg ctggaccaat acgttcagca actgggtcac
    18061 ctgtaatgta gcttgtacca aaattaaatt gcatcgcttc aagtaaccaa tttttatatt
    18121 gaattaataa tggatcattg aaaccgtact tctcattcgt ttctgcaatc aactctggtg
    18181 ttacatttgg cgtcccttgt gcatgtaaaa ttgtcacagc tggattttca tttgtaatat
    18241 acgtcaatag aaatgtcata aaacttacta caatcatcaa tggaaacatg agcgcaatac
    18301 gttttaagat aaatttgaac atctaattgc tcctttattt atactgcatt tcattgaatg
    18361 gtaattcata ctgtgattgt gtgaatgata ctttttctaa atcttttggt gcaacaactg
    18421 tcatactacc gtgtgaaata gggataaaga taccttcatc atcaatttgt ttcaaaatgt
    18481 ttttataagc gtctgaacgc tctttaccgt tttggatttt aaatgcgtca tcaatgctgt
    18541 tgtatatttt atctttgttc tcaatgcctg atgttgcact ttcataacca tttttcgctt
    18601 taaatgctgc aatagtactt tgtggatcgt acaataatcc ccaagtttgg ttgaacatta
    18661 agtcataatc accagaagta cgacgttcag caattttatc tgatgtttcg ccattgatgt
    18721 ttaacttaat acccattttc ttaaattctg cttgtaagta ttctgcttgt tctttttgac
    18781 ttgaagaacc tttgtcatag tacattgcca tttcaaggtt tttaccatct ttttgacgaa
    18841 catcgctgtc tttacctttc ttccaaccag cttcatctaa taatgattct gcttttttaa
    18901 ggtcatactt acgtgttggc atatcgaaat taatgtctgt tacatttttc gcaaataatt
    18961 gagttgctgg tttttcttga ccatctaaaa tttctttggc aattttatct ctgtttacca
    19021 tatgaccaat cgcttgtctg actgttttgt cactcacagc gttatctttt ttaccagaat
    19081 tgacaactaa cattttcgta ttcataggtt gactacgctt aacttgatag tcacctgtat
    19141 ctttcaattg ttttaaagag tctttgtcta agctatctgt acctctatca tctgtgaagg
    19201 caaagttcgt ttcacctttt ttcattgata ggaatgctgt ttcaccagca ggcattactt
    19261 ttgcttgtac tttgttaagt ttagactttt cgccccagta ttgatcattt ttgttaaagt
    19321 ctgcagactc atcttttttg tgttcaccta atttaaatgg accagtacca tcgaactttt
    19381 taacgccatc ttttgttgta ccgtttttaa agtcttttgg agacacaaat acatatggac
    19441 gaggcatcgc taattcagcc aatgcaggtt gatatgcttc tttcaaattc aattcaaccg
    19501 tgtacttatc tttaacttta acattgtcaa ttaatgtcga aatctttaac caagaatgca
    19561 attttttgtt ttcttgaact gcgtcaatat ttttcttaac tgcgtcagca tcaaatggcg
    19621 taccatcatg gaatttaacg tcatctctca aatggaacgt gtatgtcttc ccatcttcag
    19681 acacatccca ctttttagct agtaaaggct taataccatc tttcgtgtta cgtacaagcg
    19741 gctcgtatat catactttca gcagacattg atccaccgta aacatgcgga ttcatatcac
    19801 cgatatcttt aaccgtcgta tacgttaatt gcttgttttc ttttttctcc tctaaacctt
    19861 tattaccgcc acaaccagtt aaaattagcc ctgatgcaag taacattgca ctcattttag
    19921 ttagttttct catttgcttt tcctctttct aaattgataa gttgcattac ttaatcaaat
    19981 cgtaatgatt atttttctca attatttgct tatttttatg taaatgtcaa acataattca
    20041 ataatttttt tctattataa aacttcaaac ccttattatg aaagcgttct attgatttcc
    20101 aaaaattttg tgacgagtgc tttatctcct tcaaatagat ttgtattaaa ttgagaagat
    20161 aagtgttgat cttgatgcat atccttgtac gcctggcaac ttgcttcata gcgagttagg
    20221 aactgatcaa tcattggcgt tttgatacct agcattttcc caatatgctg aatcatcgcc
    20281 gttctgtaat aatcttcact tggcattctt ggaatttgaa caacatcctg ttcattttta
    20341 tagacttgct taaatggtac agctgaaaaa tcaaagtaat gtccgttttc gtctggctgt
    20401 gaaaatggat caatgaggat tgcggtatat cttacataaa gcagatattc ttgtaagata
    20461 tctggcaaga tttcgaaatg ctcaatatca ccttcatcca aagtttcagg acgtactgga
    20521 taattttcct tcaccataaa ttgaagcagg ttgactgacg gcactctaaa tgcttgtaaa
    20581 ataaccatca tttccttcca cattaaacgc atttcacgga ttagtgtcat cgttatcggt
    20641 ccttcaggaa ataacttata cacataaacc ggtacatctg ttccttcgaa aatggctttc
    20701 aatgaaaagt cattcataaa tagtggtggg tgcacataaa gcgaactgtt tcgcgtttca
    20761 gcatgcagtg gcgattcaac gacttctaat tgaatcttca attgctcagc taaagcagag
    20821 attcgttgac acattgttga gtttgaatgt gtcgatccca tgtacaattt cttttttaca
    20881 cctgttgtca acacatgatt aggcgcttct ttatcaacaa tacgtgtatc gccaagataa
    20941 gttgagaatg aaatcacttc gatatcttta ttaaatttag acataaattg ttcgacaatc
    21001 atttgcgaac caaatgtcgg tgatattaaa atgacatgtt tgacactttg caaagtttct
    21061 aacgacaatt gctgtagtgt gtcataataa gcatctgctg tgcatgccat gacaaccgtt
    21121 tcgtattcac ccttaacgtt tttaacatct ttatacaaac gattaatttc aaacttacct
    21181 tccagatgtt gatgcgcctc gttttgtatt ttgacttcaa attgtttcgc ttttttatac
    21241 gcttgatata agcgttttga tttttctgat gttgaggcac gtccaaccat atcaatctca
    21301 taatctgatt ttaaatagca aatattcgct aattggattg cgaccggacc agtgcctatc
    21361 attaataatt tagacatccg taatacctac ctttatagct gcttttttat aaagtgctat
    21421 atcaaaaatt tgctgtggtc tcatatgttt attcacacat tgccacttat cttccgctgt
    21481 ttcttgtgac ggataattaa atattgcttt gatgccatca ccaaagcgca ttgcaactac
    21541 gacattttca tttgttaaat catataattc ttctaaaatg ctgtacttta aaggaattgt
    21601 cgagctgaat atgatatgcg tcacatcttt gatatcttta agttcagata ccttttgatc
    21661 cgtaattgtt atatcttcat ttggtgctaa gacgttaacg attctgcgcc ctaggtcaac
    21721 ggcttgtgga tcaatatcaa taccgataac tgaagcacct gtttcttttg ctacttgaat
    21781 taacgtcatt ggatatgcac ctgaaccaac taacaataat ttgtcattgc tagctatact
    21841 acattgtcca aactcttcat caatacaatg ttctatattg tcaaagtaac ctgtggtaga
    21901 cgcctgtccg tctaatagac gtctcgctct aatgacttcc acttgtttaa cacactgcgc
    21961 tgtaatatat tgcagtgatt ttattaattg tgctttttct tcaacgtcac gccaagcatt
    22021 aaattgttgt tcataaatag gatttaaaat aaattcacta tagtcatcca tcaatgtttc
    22081 taatgcttcg atatattgat cgtcttgtaa tacacgctcg taatgcgctt caaacttttc
    22141 taaatattgt tgtaatatca atttgatttc attattaaag ttattcattg taggctcctt
    22201 attctatata tgcttttcct gtagctacag ttgtaacctg tcctttaatt gaagtttgat
    22261 atcccaattg atgacatcgc tttgatgtca ctaaaatact gccccctggc tgatgtactg
    22321 taaaatcttt gcatgcgtca ttacgttgat aattattaaa aaccccaatt gatgctgtac
    22381 cagaaccaca gctattttcc caaattaaac tttgaatttc tggtatatag attaatggct
    22441 gtaaaaattg acgttgttca tcaaaaagca tcatacctac tgttttatat ttgtgactcc
    22501 attgttgctc acgcacaaac gcttcaacca aatgttgaat ttctgttgtt acttgtttaa
    22561 ctggaatcac ataatgtaca tatgtttcat aaataatttc tattgctttc catgaatgat
    22621 tacccatatt aattgttgtt ggcacaacac gatgggcttg tggcatttga acttcatagt
    22681 attggcaatc atgaattgcg cattgcacta aatccgaaca gccagacacc ttcaccttaa
    22741 actgttggtc tttaagcaaa tgactttcct gcaaatgatg tatatatgac atcgtcgcat
    22801 taccgcaaaa ttcattaccg ctcataacta agtgaaaatc attaccatca tcattttgtg
    22861 ttgattctat aaagcctacc tgttcacagc atacatgtgt tgcggccatc aactgattgg
    22921 cgatagatgc atattcacta gcatcatgtt ttgaatgaac aagtatcgtc atattccccg
    22981 aaggattata cttagaaaat tctataacct gccgattcat acgacactcc tttagatgta
    23041 tttttagagc ctctgtaatt ttaattacta gaggctctta tgcagttgtt gcgaagcaaa
    23101 caactgtatt cctctaaacc tgtaattttt tagaagatga acctaatagc tctatctagt
    23161 aggtaagtca tgctaattcg caaaagcgaa aaccatggct cttatgcagt tgttgcgaag
    23221 caagcaactg tattcctcta aaactgtaat tttttagaag ctgaacctaa tagctctatc
    23281 tattaggtaa gtcatgctaa cccgcaaagt ggaaagcatg acttagtaat cggttgttgt
    23341 atcaaaatgc aaccgtaaac cttatttaaa gttgcttctt gtggctctac aagcgaatgc
    23401 agttgtccgt taactacatt cacttagatg cttttactgt tttcacctca actaaaactt
    23461 caaaaaagtt gcagaaaaca aatcgtaact attacgattt gattatatac tatattagtt
    23521 gagaatcatt gtcaataaag gttgtaaatg aatgcacatt tatattttga taatccttcc
    23581 ataaacttcg tattctcagc acattcaagt ctttttgaaa atttttatca ataattgagt
    23641 agaagttccg ttctagtcag ttcaaaattt tacgtaagtt gtctctgcgt attgctttag
    23701 tgagaataac ccacacttgt ggaaacgtcc ttttgttaat ctacaatgga tacccttgtc
    23761 tttcgctaac atttcctatt accaatcctg taacggattt tcatcgtcaa gttgttgccc
    23821 atacaatacg caccaaaaaa gctttgtaca atttcgtaca aagctttaaa aatttaaata
    23881 aaaatatcat tgttttaatg aactagccat atcagatttt atttcgtttg ctaatacttg
    23941 taattcaggg aagtaattat tagcaaagcc aattattatt ttatttttta caaacgtgtt
    24001 aatctctcga tcttctggaa tcgtattttc tattaaattc aaaagctcac
//
LOCUS       DANJFZ010000029        23377 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.029, whole genome shotgun sequence.
ACCESSION   DANJFZ010000029 DANJFZ010000000
VERSION     DANJFZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.029"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            55..2127
                     /gene="lip2"
                     /locus_tag="PWN45_001043"
     CDS             55..2127
                     /gene="lip2"
                     /locus_tag="PWN45_001043"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDL0622822.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(2369..3196)
                     /locus_tag="PWN45_001044"
     CDS             complement(2369..3196)
                     /locus_tag="PWN45_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0622823.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(3269..4468)
                     /locus_tag="PWN45_001045"
     CDS             complement(3269..4468)
                     /locus_tag="PWN45_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL0622824.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4564..4656
                     /locus_tag="PWN45_001046"
     CDS             4564..4656
                     /locus_tag="PWN45_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622825.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            4770..5771
                     /locus_tag="PWN45_001047"
     CDS             4770..5771
                     /locus_tag="PWN45_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL0622826.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5805..6137
                     /locus_tag="PWN45_001048"
     CDS             5805..6137
                     /locus_tag="PWN45_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL0622827.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6138..6938
                     /locus_tag="PWN45_001049"
     CDS             6138..6938
                     /locus_tag="PWN45_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDL0622828.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            6928..7872
                     /locus_tag="PWN45_001050"
     CDS             6928..7872
                     /locus_tag="PWN45_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL0622829.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            7850..8872
                     /locus_tag="PWN45_001051"
     CDS             7850..8872
                     /locus_tag="PWN45_001051"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL0622830.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            9187..10212
                     /locus_tag="PWN45_001052"
     CDS             9187..10212
                     /locus_tag="PWN45_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDL0622831.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(10305..11651)
                     /locus_tag="PWN45_001053"
     CDS             complement(10305..11651)
                     /locus_tag="PWN45_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0622832.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(11666..11950)
                     /locus_tag="PWN45_001054"
     CDS             complement(11666..11950)
                     /locus_tag="PWN45_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0622833.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(11952..12395)
                     /locus_tag="PWN45_001055"
     CDS             complement(11952..12395)
                     /locus_tag="PWN45_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0622834.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(12400..14355)
                     /locus_tag="PWN45_001056"
     CDS             complement(12400..14355)
                     /locus_tag="PWN45_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0622835.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            14567..14986
                     /gene="mepR"
                     /locus_tag="PWN45_001057"
     CDS             14567..14986
                     /gene="mepR"
                     /locus_tag="PWN45_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDL0622836.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15093..16448
                     /gene="mepA"
                     /locus_tag="PWN45_001058"
     CDS             15093..16448
                     /gene="mepA"
                     /locus_tag="PWN45_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDL0622837.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16552..16992
                     /locus_tag="PWN45_001059"
     CDS             16552..16992
                     /locus_tag="PWN45_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDL0622838.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17075..18433)
                     /gene="glpT"
                     /locus_tag="PWN45_001060"
     CDS             complement(17075..18433)
                     /gene="glpT"
                     /locus_tag="PWN45_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL0622839.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18741..19667
                     /locus_tag="PWN45_001061"
     CDS             18741..19667
                     /locus_tag="PWN45_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0622840.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19681..20742
                     /locus_tag="PWN45_001062"
     CDS             19681..20742
                     /locus_tag="PWN45_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL0622841.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            20756..21322
                     /locus_tag="PWN45_001063"
     CDS             20756..21322
                     /locus_tag="PWN45_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0622842.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21382..22377)
                     /locus_tag="PWN45_001064"
     CDS             complement(21382..22377)
                     /locus_tag="PWN45_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDL0622843.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22475..22771
                     /locus_tag="PWN45_001065"
     CDS             22475..22771
                     /locus_tag="PWN45_001065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622844.1"
                     /translation="MIGNLDINQSNRHYNAYWRGPNKEADEKSAYNNVQVGDGPQHRE
                     IGSVISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQVGEGQQI"
     gene            22851..>23377
                     /locus_tag="PWN45_001066"
     CDS             22851..>23377
                     /locus_tag="PWN45_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622845.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKS"
BASE COUNT         7955 a         3656 c         4584 g         7182 t
ORIGIN      
        1 cagttgtttt tcattctcaa gagggggtca aaaaaatact tttgaggtga ttatatgtta
       61 agaggacaag aagaaagaaa gtatagtatt agaaagtatt caataggcgt ggtgtcagtg
      121 ttagcggcta caatgtttgt tgtgtcatca catgaagcac aagcctcgga aaaaacatca
      181 actaatgcag cggcacaaaa agaaacacta aatcaaccgg gagaacaagg gaatgcgata
      241 acgtcacatc aaatgcagtc aggaaagcaa ttagacgata tgcataaaga gaatggtaaa
      301 agtggaacag tgacagaagg taaagatacg cttcaatcat cgaagcatca atcaacacaa
      361 aatagtaaaa caatcagaat gcaaaatgat aatcaagtaa agcaagattc tgaacgacaa
      421 ggttctaaac agtcacacca aaataatgcg actaataata ctgaacgtca aaatgatcag
      481 gttcaaaata cccatcatgc tgaacgtaat ggatcacaat cgacaacgtc acaatcgaat
      541 gatgttgata aatcacaacc atccattccg gcacaaaagg taatacccaa tcatgataaa
      601 gcagcaccaa cttcaactac acccccgtct aatgataaaa ctgcacctaa atcaacaaaa
      661 gcacaagatg caaccacgga caaacatcca aatcaacaag atacacatca acctgcgcat
      721 caaatcatag atgcaaagca agatgatact gttcgccaaa gtgaacagaa accacaagtt
      781 ggcgatttaa gtaaacatat cgatggtcaa aattccccag agaaaccgac agataaaaat
      841 actgataata aacaactaat caaagatgcg cttcaagcgc ctaaaacacg ttcgactaca
      901 aatgcagcag cagatgctaa aaaggttcga ccacttaaag cgaatcaagt acaaccactt
      961 aacaaatatc cagttgtttt tgtacatgga tttttaggat tagtaggcga taatgcacct
     1021 gctttatatc caaattattg gggtggaaat aaatttaaag ttatcgaaga attgagaaag
     1081 caaggctata atgtacatca agcaagtgta agtgcatttg gtagtaacta tgatcgcgct
     1141 gtagaacttt attattacat taaaggtggt cgcgtagatt atggcgcagc acatgcagct
     1201 aaatacggac atgagcgcta tggtaagact tataaaggaa tcatgcctaa ttgggaacct
     1261 ggtaaaaagg tacatcttgt agggcatagt atgggtggtc aaacaattcg tttaatggaa
     1321 gagtttttaa gaaatggtaa caaagaagaa attgcctatc ataaagcgca tggtggagaa
     1381 atatcaccat tattcactgg tggtcataac aatatggttg catcaatcac aacattagca
     1441 acaccacata atggttcaca agcagctgat aagtttggaa atacagaagc tgttagaaaa
     1501 atcatgttcg ctttaaatcg atttatgggt aacaagtatt cgaatatcga tttaggatta
     1561 acgcaatggg gctttaaaca attaccaaat gagagttaca ttgactatat aaaacgcgtt
     1621 agtaaaagca aaatttggac atcagacgac aatgctgcct atgatttaac gttagatggc
     1681 tctgcaaaat tgaacaacat gacaagtatg aatcctaata ttacgtatac gacttataca
     1741 ggtgtatcat ctcatactgg tccattaggt tatgaaaatc ctgatttagg tacatttttc
     1801 ttaatggcta caacgagtag aattattggt catgatgcaa gagaagaatg gcgtaaaaat
     1861 gatggtgtcg taccagtgat ttcgtcatta catccgtcca atcaaccatt tgttaatgtt
     1921 acgaatgatg aacctgccac acgcagaggt atctggcaag ttaaaccaat catacaagga
     1981 tgggatcatg tcgattttat cggtgtggac ttcctggatt tcaaacgtaa aggtgcagaa
     2041 cttgccaact tctatacagg tattataaat gacttgttgc gtgttgaagc gactgaaagt
     2101 aaaggaacac aattgaaagc aagttaaatt catcttctga atttaatatg ctatgtaaat
     2161 cgtgctgtta tcacggcaca tcagatataa gtagcatcac agtgttgaat ttaaaaatag
     2221 taaagtgaaa taaagcgcct gtctcattag cgaaaactaa agggacaggc gtatctgttt
     2281 atgagcttaa taaattgtat gaataatatg gttgatcgaa taactgttta tcatgatgat
     2341 aaattgagtt ttttaaaata atgatatatt acatcattgt tatagcgttt aagaaatcaa
     2401 caactttacg ataaatagtg attgcttcgt cattaggtct acgatcaaaa tcatgctcgt
     2461 ttttattcac gcgttcaaat gttgaatgtg gaacatgatt catgatatgt tcgctttcct
     2521 caacgggaac atcataatcg ccattacaat gcgcaatgaa aacaggtgga agtgttttaa
     2581 gttcatctgg tgcaatatta tattttgaat tagtataatc agcaatgtta atcatattta
     2641 tccatttacc tgtgccacgt gcataaacgt agattaaaaa acgttgtgcg atttgatctt
     2701 gaacaaccgg tgttggtgaa gtgagttgtg caatcattgt ttcgtttacg ctttgagcta
     2761 tttttgcgta ataactatta gttgttttaa aaggttcagt gttgatgcga ctataaccat
     2821 aaaaatcaat aacaccatca atatctctgt ctcgtgcaat taatagactt aaatatgcac
     2881 ctgatgatct gccaaaggta aaaatagggc aattagaata ttgtgattga atcgcatcga
     2941 atgatgcgta gacatcctca ataatgcaat cgagacttac ttctggtaat aaacgataac
     3001 ttagttgaat taaatcgtaa tgttccgtaa ggatatcgat atactgtggg gataaatcgt
     3061 tagctttacc gaacattaat ccaccaccgt ggatgtagac aatagcgcct tttgttggtt
     3121 gattttttgc tttaataatt gtgtaaggta atgcaaatgc atctttagta attactttat
     3181 ctttaatttc agtcacgatt taataggctc cttatttttg atattgatgt cattataaca
     3241 ctgtcttaaa tttccatgaa aaatagtctt aagacgatga gtcatgataa ttctgttcca
     3301 attgacgtaa agcgtcacgg gtatgcttct ttagaccttc cccataatcc atcattttaa
     3361 caatatcttt aaaagcagca tgtggaatgg ctaaatcttc taaatctgcc atagaaaatt
     3421 caagattgat atcatgtggt cgctgttcag caagtttatg cacaaagtca ggttctgtga
     3481 caaaaggcga agacatgccg accatatctg catgttgtaa agcatctaaa gcagactctg
     3541 gagaattaat cccgccactt gcaattaaag ggatacgacc tgctaaatgt tcatagacaa
     3601 tttggttaac tggtcgaccg aaatgatcac ctggtgtacg agacgtattt tgataaatat
     3661 gtcgacccca gctagcgatt gctaagtatt ggatgtttga aacgtccatg acccaattga
     3721 ttaattggtt gaactcgtca atggtatatc ctaaatcact gcctctggtt tcttctggcg
     3781 ttgctcgaaa tcctaaaata aaattgtcag gtgcttcttt atcaatcact tcttgtaccg
     3841 cacgcataac ttctaaacat aatcttgcac gattttttaa tgagtcggca ccgtaatggt
     3901 ctgtacgttt attcgaaaaa gttgagaaaa atgtttgaat cagcaaacgt tgtgcaatcg
     3961 aaatttccac accatcaaaa cctgctttaa tcgcgcgtaa tgtagcatcg cgatactgct
     4021 gaatgatgct attgattttc tcatgagaca tggcgataac atcgtgttca atcggtgaat
     4081 gcaatgtcat agggcttggt ccatacacct ttccaaaatt taaaatggct tgatttgaaa
     4141 aacgaccagc atgcgctagc tggataatag cgaggctacc atgttgtttc atcgtagatg
     4201 ccatgttagt taatccaggg atacaagcat catgatcaat attaaagcca tattcaaaca
     4261 attgaccata aggttcaatg taagcagcgc cggtgacttg cattccagct gaattagagc
     4321 gacgtgcagc ataagccaag tcttcttttg taatatagcc ttcttttgtt gatgtgttta
     4381 cggtcattgg tgataataca aagcgattcg aaattttgat gccattaggt aagtggattg
     4441 attgtaaaag tggtttgtat cggtacatac tatgattcct tttctattca atattgtttt
     4501 caaagtacca tggaaagaat gaataatcaa tgatgaacag tcttgataga atagaattgg
     4561 tacatggaaa gtatttttaa aattaaacta atgaatggca tttgtaggtc tgaaaatatg
     4621 aatatgaaaa agaaaaataa aggcgaaaag atataaaagt taattgaaaa acgttatcat
     4681 atacgtgggt atatgaagag ggaatggtat taagaacgct aaaatgttat gtcggtttga
     4741 catgacagga taagtttgga gatgacggat tggttaaatt aagcgtatta gactatgcct
     4801 taatagatga aggtaaggat gcacaaaagg cattgcaaga ttcagtgaca cttgcaaaat
     4861 tagcagatcg acttggcttt aagcgaattt ggtttacgga acatcataat gtaccagcgt
     4921 ttgcgtgtag tagtccagaa cttttgatga tgcatacatt ggcgcagaca aatcacatac
     4981 gagttggctc tggtggtgtg atgctgccgc actatcgacc ttataaaatt gctgagcatt
     5041 ttagaatgat ggcagcgtta tatccaaatc gtattgattt aggtattggt aataatccag
     5101 gtactactat ggtaaagcaa gctttagatg gaataaatcc tacatatgat agttacgatg
     5161 aatcgatttc gttattacgt gattatctta caataaagga taaaccaagt gcgcatacgt
     5221 taggtgtcca accacacatt gatcattttc cagaaatgtg gttattaagt agtagcgcaa
     5281 catctgccaa aatagctgcc gaactaggta tagggctttc tgttggaaca tttttgctac
     5341 cagatataaa tgcgatacat gcagcgaagg ataacattga tatttacaaa aaacatttcc
     5401 aagcatcaac gattaaaatg gacgcaaagg tgatggcatc tgtatttgtc attgtagctg
     5461 ataacgaagc ggaagtagca gcattacaac atgccttaga tgtttggtta ttaggtaaat
     5521 tacaatttgc agaatttgaa gattttcctt cagtagacac agcacaaaag tataagctta
     5581 atgatcgaga caaagagatg attcaagcac atcaagcacg catcattgca ggtacacaag
     5641 aaaaggttaa agcacaatta gatgatttca ttgctacgtt tgaagttgat gaggtgttag
     5701 tagcaccgct tattccaggt attgaacagc gttgtaaaac attaaaatta ctcgcggaaa
     5761 tttatttgta gcattttaaa tagaagagaa aggatgaaga taagatgaaa aagttagcca
     5821 attatttatg ggtagaaaaa gtaggagatt tgtatgtgtt tagtatgaca cctgaattgc
     5881 aagatgatat tgggacagta ggttatgttg aattcgtaag tccagatgaa gttaaagtgg
     5941 atgatgaaat tgtgagtatc gaagcatcga aaacggtcat tgatgtgcaa acgccattgt
     6001 caggaacgat tattgagcga aatacaaaag cggaagaaga accgacaatt ttaaactctg
     6061 aaaaaccaga agaaaattgg ttgttcaaat tggatgatgt cgataaagaa gcattcctag
     6121 cattaccgga ggcttaaatg gaaacgttaa aatcaaataa agcgagactt gaatatttaa
     6181 tcaatgatat gcatcgagag agaaatgaca atgacgtatt ggtaatgcca tcttcatttg
     6241 aagatttgtg ggaattatat cgaggcttag caaatgtcag accggcatta cctgtaagtg
     6301 atgaatattt agctgtacaa gatgctatgt taagtgattt gaatcgtcaa catgttacgg
     6361 atttgaagga tttgaagccg ataaaaggtg acaatatctt tgtttggcaa ggtgatatca
     6421 cgacgttaaa aatcgatgct attgttaatg ctgcaaatag tcgttttcta ggatgtatgc
     6481 aagctaatca tgactgcatt gataatatta ttcatacaaa agcgggtgtt caagttcgac
     6541 ttgattgtgc agagatcatt cgacaacaag ggcgcaatga aggtgtaggt aaagccaaaa
     6601 taacacgtgg atataatttg tcagcaaagt atataattca tacggttggt ccgcaaatac
     6661 gtcgattgcc tgtttcaaag atgaatcagg acttgttagc taaatgttat cttagctgtc
     6721 ttaaattggc tgatcaacat agtttaaatc atgtcgcttt ttgctgtata tctacaggtg
     6781 tatttgcttt tcctcaagat gaagcagcag aaattgctgt tcgaacagta gaaagctatc
     6841 tcaaagaaac aaattcaaca ttgaaagtcg tgttcaatgt atttacagat aaggatttac
     6901 aactgtataa ggaggcattt aaccgtgatg cagagtagta agtggaatgc aatgtctctg
     6961 ttaatggatg acaagacaaa gcaggctgaa gtattgcgta ctgcgattga tgaagcagat
     7021 gcgatagtga ttggaattgg tgcaggcatg tctgcatctg acggatttac atatgtagga
     7081 gagcgtttta cggaaaattt cccagatttt attgaaaaat atcgcttctt tgatatgttg
     7141 caagcgagtt tacatcctta tggcagttgg caagagtatt gggcatttga gagtcgtttt
     7201 attacattaa actatttaga tcaacctgta ggtcagtctt acctcgcttt aaaatccttg
     7261 gtggaaggta aacagtacca cattataact acgaatgcag ataatgcttt cgatgtagct
     7321 gattatgata tgactcatgt atttcatata caaggggagt atatactgca acagtgtagt
     7381 cagcattgtc atgctcaaac gtatcgcaat gatgatttaa ttcgtaaaat ggttgttgcg
     7441 caacaagata tgcttatacc ttgggagatg attccaagat gtccaaaatg tgatgcccca
     7501 atggaagtga ataaacgtaa agcggaagtt gggatggttg aagatgctga atttcatgcg
     7561 caactacatc gttataatgc ttttctagag caacatcaag atgataaagt gttgtatttg
     7621 gaaattggaa ttggttatac tacaccacaa tttgtgaagc atccttttca gcgtatgaca
     7681 cgtaaaaatg aaaatgccct ttatatgacg atgaataaaa aggcatatcg cattccgaat
     7741 tcaattcaag aacgtaccat acatttaact gaggatatct caacattgat tacagcagca
     7801 ctccggaacg acagcacaac gaaaaataac aacattggag agacagaaga tgtacttaat
     7861 agaaccgatt agaaatggag aatatattac tgatggtgcg attgcactcg ctatgcaagt
     7921 ttatgttaac cagcatatct ttttagatga agatatttta ttcccttatt attgtgatcc
     7981 aaaagtggaa attggacgtt ttcaaaatac tgctatagaa gtgaatcaag attatataga
     8041 taaacacagt attcaagtag ttcgccgaga tactggtggt ggcgctgtgt atgttgataa
     8101 aggtgccgtt aatatgtgtt gtattttaga acaagacact tcaatttatg gtgattttca
     8161 acgattttat caaccagcta taaaggcgtt gcatacatta ggtgcaacag atgtggtaca
     8221 aagcggtaga aatgatttaa cattgaatgg taaaaaagtg tcaggcgccg caatgacatt
     8281 aatgaataat cgtatttatg gcggttattc gctattactt gatgttaatt atgaagcaat
     8341 ggataaagtg ttaaagccta atcgcaaaaa gattgcatcg aaagggatta aatctgtgcg
     8401 cgcacgtgtt ggtcatctta gagaagcact ggatgaaaag tatcgtgata taaccattga
     8461 agaatttaaa aatttaatgg tgacgcagat tttgggaatc gatgacatta aagaggcgaa
     8521 acgatatgaa ttaacggatg cagattggga agcgattgat gaattagctg ataaaaagta
     8581 taaaaattgg gattggaatt atggcaagtc acccaaatat gaatacaatc gaagtgaaag
     8641 attatcttca ggtacggtag acataacaat ttctgttgaa caaaatcgta tcgcagattg
     8701 tcgtatttat ggggatttct ttggacaagg tgatataaaa gatgtggaag aagcattaca
     8761 aggaacaaaa atgacaagag aagatttaac gcatcagtta aagcaattag acatcgttta
     8821 ttattttagc aatgttacgg tagaagcatt agtggatatg attttaagtt aatattgtta
     8881 ttttatgtat gctgaatcat tggaagtgtt tgcttgctct tgaaaaggtg acaatagtgt
     8941 ttggtgaagg ttgaacatat gagtggaaat tattgccttt aactattcaa agtatgatat
     9001 atatatggtt tttgtttcta aatgattggg tatttgaaaa tagatgagtt taatatttta
     9061 aggaatataa tgatgtttac ttttataatt catatagaat attaagcaat ataagtctgt
     9121 tgatatatac aaaatataat gactgctata atgagtaatc aatagacaca aagaggagat
     9181 tatgtgatga ataataaagt attagcaacc ggtggtacag ggtttgttgg catgcgaatt
     9241 atttcacgat tattagaaca aggttatgac gtacaaacga cgatacgtga tttaagtaaa
     9301 gctgataaag taattaaaac aatgcaagac aatggcattt ccacagagcg attaatgttt
     9361 gtcgaagcgg atttatcaca agatgaacat tgggatgaag caatgaaaga ttgcaagtat
     9421 gtcttgagtg tagcatctcc ggtgtttttc ggtaaaacag acgatgcaga agtgatggcg
     9481 aagcctgcaa ttgaaggtat acaacgtatt ttaagagctg cagaacatgc gggtgttaaa
     9541 cgtgtggtaa tgactgcaaa ctttggtgca gttggtttta gtaataaaga taaaaattca
     9601 atcacaaatg aaagtcattg gacaaatgaa gatgaaccag gcttatcagt atatgaaaaa
     9661 tcaaaattgt tagctgaaaa ggcagcgtgg gattttgttg agaatgaaaa tacaacagta
     9721 gaatttgcca caatcaatcc agttgcaatt tttgggccat cattagatgc acacgtttca
     9781 ggaagctttc atttattaga aaatttattg aatggttcaa tgaaacgtgt accgcaaatt
     9841 ccgttaaatg ttgttgatgt gagagacgta gctgaactgc acattttggc aatgacaaat
     9901 gaacaagcta atggcaagcg atttattgcg acggctgatg gacaaattaa tttgttggaa
     9961 attgcaaaat taattaaaga aaagagacct gaaatagctc aaaaagtttc tactaaaaaa
    10021 ttaccagact ttattttgag tctaggtgct aaatttaatc atcaagctaa agaaggtaaa
    10081 cttttattag atatgaaccg caatgtaagt aacgaacgtg caaaaacatt acttggttgg
    10141 gaaccgattg cgacacaaga agaagcaatt ttagcagctg tcgatagtat ggctaagtat
    10201 catttaatat aatacaaaac accgtccata agtaagtggc tttcctcttg ataaaagggg
    10261 cattcactca atatgtgacg gtgttttata tttctacgtt aacactattg ctgttctttt
    10321 tgacggcctt ttaataaaat tgttgtcgca cctacgataa taataaatag aatcgcacca
    10381 aataatccca tatattttac tgcgttaccg aacacgatac cgacagctaa aaagtctgta
    10441 tctgagaatg ttgttgcagc accacctaat tcgcctaaaa atggcaagaa taataatggt
    10501 aaaaacgtga ttaggatacc atttagagcg gcgccagcaa tagcaccttt aataccgcct
    10561 cttgcattac cgaatacagc agccgttgca cctaagaaga agtgtgcaac tacgccaggt
    10621 aaaatgacga cgccaccaaa taagaataag ataaacatac cgatgacacc tgtaataaag
    10681 ctgacaaaga atccaattaa tactgcattt tgtgcataag ggaacacaat agggcagtct
    10741 aatgcaggtt tagaatttgg tacaagcttt tcagaaattc ctttaaatgc tgggacgatt
    10801 tcagctaaga ttaaacgaac gcccgttaaa ataataaata caccagcagc aaatgtcaca
    10861 ccttgaatta atgaaaagac aataaagttt tgaccatcac taatagattc gtgtacataa
    10921 ctaacgcccg caaataagca tgcgatgaag taaagtaatg ccatcgtaat cgagatacta
    10981 attgtacttt ctcgtaagaa acttaagcct tttggaaatt taatctcttc cgttgattta
    11041 gacttacctt tgaataattg acctacagca cctgcggcaa agtaactgat tgagccaaaa
    11101 tgacctaaag ctacttggtc attccctgta atttttcgca tcgtaggttg gagtaatgca
    11161 ggtaatactg ccatgattaa tcctaatacg agtgcgccga taacaatcgt tagccagcct
    11221 ttaatatgac tgactgttaa aatgattgct aaaaacgcag ccatgtaaaa tgtatgatga
    11281 cctgttaaaa agatatattt taaattagtg aagcgggcaa ttaaaatatt aacaatcatg
    11341 ccacagacca tgatgagtgc agctgttgtt ccaaaatctt ttaaggctag tgagacgata
    11401 gcttcgttgt taggtacgat accttgcaca ccaaatgcgt gttggaatat tttgccgaat
    11461 ggttcaagag atcgaacgac gacatcagca cctgcactta aaattaagaa gcctaatatc
    11521 gttttaatgg ttcctgaagt gatcgttgcg gcaggttttt tctgaacgat taaacctata
    11581 aaggcaatca gtgcaacaag aatggctggt tgacttaaaa tatcgactat aaaattaagg
    11641 attgcttgca taggtcgtac ctcctttaaa tcatgttaag ttgttgtaat ttttctgaga
    11701 gcttttgttg taattcagct ttatctaaaa tattatcaag aactaagaca tcccctagac
    11761 gttcggcatt ttcagctaaa tctctaccac aaataaacaa gtcagccatc tctggacttg
    11821 ctgtcataat gtcactatgt tcaacttcga tatcagatgg tgcattaagt tgcctaagtg
    11881 cttcttgtgc gttcatttct accataaaac tacttcctaa accgtggcca catactacta
    11941 aaattttcat attaatcatg ctcctttaaa atgtttttaa tgtcttgtgc atttgttgca
    12001 gttaatagtt gctggactgt ttggttatcg cccagtacgg ttgctaaatt ttgtaataca
    12061 gataagtgtg aatgattgtc gatggcactc aatacaaaaa tgagagatgc gtagtgatct
    12121 tcatcacaaa atgccacatg ttgattcaac tttaatagac ttaaaccaac ttgatgtacg
    12181 tcattgttcg gtcttgcatg tgcaattgca atttcaggtg cgataacgat ataaggtcca
    12241 agttcattaa cgctatcaat cattgcttga acatagcctt gttcaataat ttgttcttgt
    12301 agtaatggct gagaagctat agttatagct tcagtccaat catttacttg ttcttttaca
    12361 atgatgcgtg ttgttgacaa aatgtctaat gacacgttat taagcctcct ttgtcatagt
    12421 taaagcaatg tgttgtttaa ttttaaaaat attcccatct aagaaatctt gtcgatataa
    12481 gtcgttgctt aagcattcgc ttaactgtcc caatgccttt aaatgtgcat tggggtggtc
    12541 cgttgctaat gtaattacaa ggtgaacggg atcgttagct ttactaccaa agataatccc
    12601 ttcagtgaaa tatgttagtg cgaaacctac accattctgt acataatcag taccagcgtg
    12661 aataagtgca atatgtggac taatgaccat atatgacccg aattgttcaa attgttttaa
    12721 aattgcagct gtataatttg aatagacaat gccatcattg attaaaggtt gcacagccac
    12781 tgcaattgcg gattcaattg ataatggttg tttatttata atgatgcgat gttcaggcaa
    12841 taaatctgcg agtgacttgc catcagttgc cattttcatg actcgttgtt ctcttgagtc
    12901 attgataatt tgattcaatt tttgacgaga ttgttgattg ataaatggat cgacatgaat
    12961 aactggtaca gctgatattt cacaaggtac tgttgaaatg acataatcaa tgttatcttg
    13021 caataatcga ctttcttcca attgataaat ggaataggca tcccaaatgt gaaactcagg
    13081 atacaggtga tttagttttg attttaaaag ttgtgacgtg cctataccag aaccacatag
    13141 taagacaacc ttaatcattg attgtttatg tgttgcaaca cgctctatac ttgatgcgaa
    13201 gtgaattgta atgtatgtta attcatcttc gttgaagcga atagcagcat cttgttcaat
    13261 tggactaata tgcttgctaa cggcttcaat gatttgagga tagcgacgca taacttcttg
    13321 cctcaaagga ttaggttgta gcatatcgta tttaatacga tgtatagctg gtttgatatg
    13381 tgtgatcaga ctggtatgta acttgttgtc ttttgacata tcaatgccta attcttggct
    13441 aacacaagtg atcaattcat gtatattttg cgataaatca tggtattcaa aggtaattga
    13501 agatgctgta tgttcagtca ttttagagcc tagtaaatgt aacgtgataa agataatttc
    13561 agactctgga aatgtgacat tacaactgcg ttctaagttt tctatcattt ttgaagcaat
    13621 agcatactga ttagtatgtc gccatttatc aatttcattg ataggtatat cgaacgaaaa
    13681 attttcattt aaacgctgaa tggcaatgag tatatgatag attaagccat cgatagccga
    13741 ctgaactaaa tgataatttt cactatttaa tgtcttaata atggcacggc gaaccaatgc
    13801 gattgattct gaattaaaga tatccgcctc tataaaaggt gcagcttgtt tcatatattg
    13861 atgtataaag tgtgcatacg ctttacgata atgatcttcc tcaccaataa tattgaatcc
    13921 tttattgtgg acataattta actttaaatg gtattgatct agttgggctt gaatcatttt
    13981 aatatcatct gcaattgtcc gacgcgaaac attaacatct tgcgcaagtt gctttgttga
    14041 aacaggatcg gttgtttcga ataactttaa agcgatatgt gtgagtcgtt catcttttga
    14101 aaaatgaatt tgatttgtta gattgtgctc taattcattt aatagtgtcg cgtgagctgt
    14161 tgttactttg atgcctgcag ctttattacg gctgacttgg taatgataag tttcagcata
    14221 ttgctcaata tatgctatat catattgaat ggtacgaggt gatacaccaa gttgattagc
    14281 aatggtattg attggaataa acgtttgctc atgaattaaa agatacaaaa tttcgatttg
    14341 tctataactt aacaacgtaa tatcctccta tttgtaattg taagcgattt cttaaaaacg
    14401 tagatatgca atctctttca tattttaatc cgaaaaattg catatcaaaa tgtttatggc
    14461 gcaagatttt ataggaactt ttaaaataaa ttagatattc atgttgtcaa tttaaaaatg
    14521 tcgcagtata tttagttaga catctaacga aatggtggtg caataaatgg aattcactta
    14581 ttcgtattta tttagaatga ttagtcatga gatgaaacaa aaggctgatc aaaagttaga
    14641 gcaatttgat attacaaatg agcaaggtca tacgttaggt tatctttatg cacatcaaca
    14701 agatggactg acacaaaatg atattgctaa agcattacaa cgaacaggtc caactgtcag
    14761 taatttatta aggaaccttg aacgtaaaaa gctgatctat cgctatgtcg atgcacaaga
    14821 tacgagaaga aagaatatag ggctgactac ctctgggatt aaactcgtag aagcattcac
    14881 ttcgatattt gatgaaatgg aacaaacact cgtatcgcag ttatctgaag aagaaaatga
    14941 acaaatgaaa gcaaacttaa ctaaaatgtt atctagttta caataaatga taagtgtgac
    15001 tggtagaaat cagtcacttt gtctttaata ttatagttag atatctaatt gttagtaagc
    15061 taattattgg aaaagacaag gagtattgaa caatgaaaga cgaacaatta tattattttg
    15121 agaaatcgcc agtatttaaa gcgatgatgc atttctcatt gccaatgatg atagggactt
    15181 tattaagcgt tatttatggc atattaaata tttactttat aggattttta gaagatagcc
    15241 acatgatttc tgctatctct ctaacactgc cagtatttgc tatcttaatg gggttaggta
    15301 atttatttgg cgttggtgca ggaacttata tttcacgttt attaggtgcg aaagactata
    15361 gtaagagtaa atttgtaagt agtttctcta tttatggtgg tattgcacta ggacttatcg
    15421 tgattttagt tactttacca ttcagtgatc aaatcgcagc aattttaggg gcgagaggtg
    15481 aaacgttagc tttaacaagt aattatttga aagtaatgtt tttaagtgca ccttttgtaa
    15541 ttttgttctt catattagaa caatttgcac gtgcaattgg ggcaccaatg gtttctatga
    15601 ttggtatgtt agctagtgta ggcttaaata ttattttaga tccaatttta atttttggtt
    15661 ttgatttaaa cgttgttggt gcagctttgg gtactgcaat cagtaatgtt gctgctgctc
    15721 tgttctttat catttatttt atgaaaaata gtgacgttgt gtcagttaat attaaacttg
    15781 cgaaacctaa taaagaaatg ctttctgaaa tctttaaaat cggtattcct gcatttttaa
    15841 tgagtatctt aatgggattc acaggattag ttttaaattt atttttagca cattatggaa
    15901 acttcgcgat tgcaagttat ggtatctcat ttagacttgt gcaatttcca gaacttatta
    15961 tcatgggatt atgtgaaggt gttgtaccac taattgcata taactttatg gcaaataaag
    16021 gccgtatgaa agacgttatc aaagcagtta tcatgtctat cggcgttatc tttgttgtat
    16081 gtatgagtgc tgtatttaca attggacatc atatggtcgg actatttact actgatcaag
    16141 ccattgttga gatggcgaca tttattttga aagtaacaat ggcatcatta ttattaaatg
    16201 gtataggttt cttgtttact ggtatgcttc aagcgactgg gcaaggtcgt ggtgctacaa
    16261 ttatggccat tttacaaggt gcaattatca ttccagtatt atttattatg aatgctttgt
    16321 ttggactaac aggtgtcatt tggtcattat taattgctga gtcactttgt gctttagcag
    16381 caatgttaat cgtctattta ttacgtgatc gtttgacagt tgatacatct gaattaatag
    16441 aaggttaaat atttcgtcca cttctggctg agtatatttc ggtcggaagt gtatttttcg
    16501 aaaaaaataa atatatgata cgattatgaa aaaataaagt gagattggca tatgtgtaaa
    16561 tctaaaatac tgttgaaaaa tatttttagt gaagaatcag aagttaaaga tttaactgaa
    16621 gaaaaatata atcaagatta cgaagcatta acatttagct ttaaagagga aacatatcaa
    16681 agtaggttag ctaagaaaac accgactaaa tcgggatatt tcgtgacatg ttggacaaaa
    16741 gacgaagata attataatcg gccatacaaa attgaagagt ttgctgatta cctgattgtt
    16801 gctgttatcg atgatgaatt aaatggctac tttctatttc ctagggaatt attggtagaa
    16861 aaaggtatct tagcttcatc taagtatcaa gggaaaatgg cttttagagt ttatcctaag
    16921 tggtgtaatc aattgaataa aacagcaggg caaacacaaa agtggcaatg taaatatttt
    16981 tttgaatact aataaaaagt catatggttg aacttataag agatgaatat atcatctttc
    17041 gataagataa ccatatgact ttatttttta tcatttaatg atgaacggtt tcttgtccta
    17101 ctttattcca agtgaggata aagctcaaca ttgcaaacac actaattgct gttaataaaa
    17161 taaaaccgac atcccatccg aatttatcaa ctacagcacc taagacgatg ttggccatta
    17221 cagcaccaaa cagataacca aataatcctg ttaatccagc tgctgtgcca gctgcttttt
    17281 taggtacata atctaatgct tgtaaaccaa ttaacataac tggtccatat attaagaaac
    17341 caatggcaat taatgagaca ttgtctaacc aagcattgcc tggaggattt aaccaataaa
    17401 ttaatacaaa tactgtgaca cctaacataa agaagaaacc tgcaggtcca cgacgacctt
    17461 tgaataattt atcagaaatg taaccacata ataatgtacc aggaattcca gcccattcgt
    17521 ataagaagta tgcccaacct gatgctttta agtcgaaatg tttttcttca cttaagtaga
    17581 ctggcgccca atcaagtaca ccataacgca cgaaataaac aaatatattt gcaaaggcaa
    17641 ttgcccatac ccatttattg ttcagtacat atttaaataa aatttctttt gtagttaatt
    17701 ctgtttctaa tgttttctta tcgcttgtag caaagtcatt tttataaatt tcgattggag
    17761 gtaaaccttg agattgaggt gtgtctctaa tcaatacgta tgaaattgcg gcaatgataa
    17821 gtgctaagag tgcagggtaa atgaatacac cttcgaaacc ttttaaataa ccaaagttga
    17881 taaatgcagt tgttgtaata ccccaagcag caataggtgc cataatacct ccaccaacat
    17941 tatgcgcaac gttccaaagg gcagtcttac ttccgcgttc acttacacta aaccagtgaa
    18001 cgagaacacg gcctgaaggt ggccagccca taccttgaaa ccatccattt aagaataata
    18061 ggacaaacat aataccgata cctgatgtga agaacggtac aaatcccatt aacaaattga
    18121 cgatagcagt gagtgctaat ccaagaacta agaatatccg agcattgctc cgatcactta
    18181 cagtacccat aaagaactta ctaaatccat atgcgatgga aacagcagaa agtgcaaaac
    18241 ctagttccgc ttttgtaaaa ccttgctctt gcaatgccgg catcgctaac gaaaagtttt
    18301 tacgtaataa atagtaccca gcataaccga tgaaaatacc aagaaatact tggagacgta
    18361 atcgtttata ggtatcatct atctgatttt ctggcaaagg cttaatatgc tttgcaggtt
    18421 taagaaaatt cataaaatcc tccttaatat gtatttatat gcattttgtg cgataaactt
    18481 atattagaca tgtatgaaag cgttgtcaat atgcttttgt gaaatcttgc atatttagtt
    18541 attgaagtat tttataaaca tttagaaaaa ataaattgct acacataaat ttataattat
    18601 gcaattatta tgtcgttata catactagct gtgtatctac gaatgagtac aaacatattt
    18661 ttatttgcaa ggggtaaatg gcatataact atctttttta tgtaagctgg tataaaattt
    18721 atactaatag gagggatagt atgaatatag tagggcatca tcacatatcc atgtatacaa
    18781 aagatgcaaa acgtaataag gatttttaca caaatgtcct tggattacga ttagttgaaa
    18841 agtcggttaa tcaagacaat ccttcaatgt atcatttgtt ttatggggac gaagtaggta
    18901 cagccggaac aattttaagc ttttttgaaa ttcccaatgc gggtcataag cagccaggta
    18961 ctgaaacgat ttatcgattt tcattattag taccaaatca agcggcactt cattattttg
    19021 aaaaacgtct tgagaataat ggtattaagt ctgaacgttt gtactatctt ggacaagaag
    19081 gtgttgtctt taaagatgaa gacgacttag aaatcatatt gcttgttaat gatagttttg
    19141 aagtaccaca tcaatggcaa cataacgctt atagtgaaat acctcaagca tatcaaattt
    19201 taggaatagg gccagtcgaa ttaagagtta gaaatgcagc gcgtacggta gaatttttgg
    19261 aaaatgtctt aggttatcgc aaaagagata ataaatcatt cgatgtgctg acattagcac
    19321 cacaaggttt atattcggat tttgtagtta ttgagcaaca gggacaacgt gaaagacctg
    19381 gacgaggtta tatccatcat attgcagtta atacaccaca aatgagtgac ttagatgcaa
    19441 tttacaagaa attacaacaa caaccacaaa gtaattcagg tataattgat cgctatttct
    19501 ttaaatcatt atactatcgc cataattcaa ttatgtatga atttgcgact gaagcgcctg
    19561 gatttactat tgatacacct gttgaacaat taggaagtca attgaacttg cctgactttt
    19621 tagaagcaga acgtgaacaa attgaaagta agttacacga aatataaagg agaatgttta
    19681 atggccaaat tagaaatgaa taaaaatacg cctcttgagt ttggtttgta ttccttaggt
    19741 gatcatttat tgaatccatt gaaaggtgaa aaagttagtt atgagcaacg tattaatgaa
    19801 attattgaag caagtaaatt agcagatgaa gcaggtattg atgtttttgc agttggtgaa
    19861 agtcatcagg agcattttac aacacaggca catacggttg tgttaggtgc aattgcccaa
    19921 gcgacaaagc atattaaagt ttcaagttct tcaacgatta ttagtgcaac agatcctgta
    19981 agagtatttg aagacttcgc gacattagat ttgatttctc atggtagagc cgaaattgta
    20041 gctggcagag catcaagaac aggtattttt gacttgtttg gctatgattt aaaagactat
    20101 gatgaattgt ttgaagaaaa attaggttta cttttagagt taaataaaac tgagcgtatt
    20161 acttggtctg gaaaatatcg tccagaactt agaaatatga aaatattccc aagaccaatc
    20221 gataatatat tgccaatatg gcgtgctgtt ggtggtccac ctgcaagtgc tattaaagcg
    20281 ggaaaacaag gtgtgccaat gatgattaca acccttggtg gcccagcaat gaactttaaa
    20341 ggttctatag atgcttatcg tcaagcggca actgaagcag gtttcgatgc ttcgcctaag
    20401 tctttaccag taagtacagc gagtctgttt tatacagctg aaacaactca ggatgctatg
    20461 agagaatttt atccacattt gaatacaggg atgtcattta ttcgtggtgt tggttatccg
    20521 aaacagcaat ttgctaattc gtcagattat cgagaagcgc taatggttgg aagcccgcaa
    20581 caaattattg aaaagatatt gtatcaacac gagttgtatg gtcatcaacg ttttatggca
    20641 cagcttgatt ttggcggtgt gccatttgaa aatgttatga agaatattga gttaattggc
    20701 aacgacatta taccggcgat taaaaagcat ttatcaaaat aggaggggcg tcatcatgaa
    20761 tattgtatta ttgtcaggtt ccacagtagg ttctaaaacg agaattgcta tggatgattt
    20821 aaaaaatgaa ctagaagtca tcaatgaggg acatcaaata gagttgatgg atttacgaga
    20881 acttgaatta gaatttagcg ttggaaagaa ttatctagat actacaggag atgtatataa
    20941 attaacgacg tcgttaatgc aggctgatgt gatttttatt ggttttccaa tttttcaagc
    21001 ttccatccct ggtgctttga aaaatgtgtt tgatctactt ccagtcaatg cgtttcgtga
    21061 caaggtaata ggacttgtag cgacagcagg ttctagtaaa cattatttaa ttcctgaaat
    21121 gcatttaaaa ccaatattga gttacatgaa agcacatacg atgcaaacgt atgtatttat
    21181 tgaagagaaa gatttttcaa atcaacaaat tgtcaatgat gatgttgtat ttcggttaaa
    21241 agcgttggca caatccacaa tgcgaactgc caaagtacaa caacaagtgt ttgaagaaga
    21301 aaacaaccaa tacgactttt aaagtataaa aataagacgc tcggcacact aaatttgtaa
    21361 gtgtttgagc gtcttttcat attaactata tagccaatga acgacgataa aggcaagtga
    21421 tgacaagcat attgaggtaa taatgattgt cataagcggt ttaagtgcgc gatttttaag
    21481 atctttaaat gcaacattta accctaaagc aaccatggcc attaataagc aaattgttga
    21541 tacagtattt aaaatattta gcaatgctga cggaatagtt acatatgtat tcactaaggc
    21601 cataatgaca aatccaatta aaaagtatgg aatgcttatt cgacccttgc tagatgattc
    21661 tgatgaacgg aaacgcataa ttaaaataag tacgatggtt aatggaatca gtaagaatac
    21721 tctaccaagt ttaccaagaa gtgcaatttt aagtgcatca ctaccaccaa agccaccagc
    21781 taagacaacg tgtgcaattt catgaagact aacaccagac caagcgccat aaacatttgt
    21841 cgtcattgaa aagatagcgt agatagctgt atatataagt gaaaatatcg taccaatcaa
    21901 tgcgatgata ccgatactaa tagctgtatc cttttcacgt gatttgaata ttggagcgac
    21961 tgcggcaata gcagcagcac cacaaacgcc tgtgccgaca cctagtaata atgcgatgtt
    22021 tttgtcacca tgcaacagtt tgttgacaaa gagcatcatt acaatactga aaataacgac
    22081 acctacatcg atggctaata gtttactacc ttgaccgata atatcgaata tattgagttt
    22141 aagtccatat aggatgattg caaatcttaa taaatattta gatgaaaacg taatacctga
    22201 gctatattgt tcaggatatc ctctaaagtg acgatataga atagcgatta atatcgcgat
    22261 agttaatgcg ccaaccttat ctaggattgg caatttagct gctaaaaagc taaataatgc
    22321 gactataaat gttagtgata gcccaatcat aaaatgctta tttttcaatg atgccatgag
    22381 cagtgcctcc tttaatagca ttttagcact gttttgtcgt atttttaaat ataaatttgg
    22441 aatgaataat aaagtagtga ttaaattaag ttgtgtgata ggaaacttgg acatcaatca
    22501 aagtaatagg cactacaacg cttattggcg gggccccaac aaagaagctg acgaaaagtc
    22561 agcttacaat aatgtgcaag ttggggatgg gccccaacat agagaaattg ggtccgtaat
    22621 ttctacagac aatgcaagtt ggcggggccc caacatagag aatttcgaaa agaaattcta
    22681 caagcaatgc aagttggcgg ggccccaaca tagagaattt cgaaaagaaa ttctacaagc
    22741 aatgcaagtt ggggaaggac aacaaattta agatacaatg cgtaacatta atatgttatt
    22801 ataatgataa tttacagaat tatatgaaaa atgaatgagg atgtgatggt atgtttggaa
    22861 tgaaagtgaa tgaacaaata acattaaaaa ttttagaagc tcatgacaca gaagcgcttt
    22921 tcaatttagt caatcgttca agaaattcac ttagggaatg gttaccttgg gtagatgcaa
    22981 ctgagcaacc atcagatacg cgtgcattta ttaaaagagg acttttgcaa tttgctgatg
    23041 gtaatggatt tcagtgtggc atttggtatg aaggaacgct agttggtgtc atcggtttac
    23101 atgaaattaa tcacatgcac agaaaaactt cattagggta ctatttagat aaagaatttg
    23161 agggtcatgg gattatgaca caagcagttg aggcattgat aaagtattgt ttcgaagagc
    23221 ttgacttaaa ccgaattgag attagtgccg cagttaataa tgaaaaaagc cgggctattc
    23281 ctgaaaggct gggatttact agagaaggta tgttacgtga caatgaatta ctaaatggta
    23341 tttattcatc gagttacatc tatagtttat taaaatc
//
LOCUS       DANJFZ010000030        23198 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.030, whole genome shotgun sequence.
ACCESSION   DANJFZ010000030 DANJFZ010000000
VERSION     DANJFZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.030"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(168..1181)
                     /locus_tag="PWN45_001067"
     CDS             complement(168..1181)
                     /locus_tag="PWN45_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL0622846.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            1493..2635
                     /locus_tag="PWN45_001068"
     CDS             1493..2635
                     /locus_tag="PWN45_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0622847.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2636..3754
                     /gene="mnmA"
                     /locus_tag="PWN45_001069"
     CDS             2636..3754
                     /gene="mnmA"
                     /locus_tag="PWN45_001069"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL0622848.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            4216..4884
                     /locus_tag="PWN45_001070"
     CDS             4216..4884
                     /locus_tag="PWN45_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0622849.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            4886..7363
                     /locus_tag="PWN45_001071"
     CDS             4886..7363
                     /locus_tag="PWN45_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL0622850.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            7706..10336
                     /gene="alaS"
                     /locus_tag="PWN45_001072"
     CDS             7706..10336
                     /gene="alaS"
                     /locus_tag="PWN45_001072"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL0622851.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            10399..10659
                     /locus_tag="PWN45_001073"
     CDS             10399..10659
                     /locus_tag="PWN45_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL0622852.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            10663..11091
                     /gene="ruvX"
                     /locus_tag="PWN45_001074"
     CDS             10663..11091
                     /gene="ruvX"
                     /locus_tag="PWN45_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL0622853.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            11106..11414
                     /locus_tag="PWN45_001075"
     CDS             11106..11414
                     /locus_tag="PWN45_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL0622854.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            11699..12337
                     /locus_tag="PWN45_001076"
     CDS             11699..12337
                     /locus_tag="PWN45_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL0622855.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            12340..13263
                     /locus_tag="PWN45_001077"
     CDS             12340..13263
                     /locus_tag="PWN45_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0622856.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            13275..14543
                     /locus_tag="PWN45_001078"
     CDS             13275..14543
                     /locus_tag="PWN45_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0622857.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            14543..15166
                     /gene="udk"
                     /locus_tag="PWN45_001079"
     CDS             14543..15166
                     /gene="udk"
                     /locus_tag="PWN45_001079"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL0622858.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            15194..15670
                     /gene="greA"
                     /locus_tag="PWN45_001080"
     CDS             15194..15670
                     /gene="greA"
                     /locus_tag="PWN45_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL0622859.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            15996..16730
                     /gene="pxpB"
                     /locus_tag="PWN45_001081"
     CDS             15996..16730
                     /gene="pxpB"
                     /locus_tag="PWN45_001081"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL0622860.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            16720..17730
                     /locus_tag="PWN45_001082"
     CDS             16720..17730
                     /locus_tag="PWN45_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL0622861.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            17732..18181
                     /locus_tag="PWN45_001083"
     CDS             17732..18181
                     /locus_tag="PWN45_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDL0622862.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            18195..19556
                     /locus_tag="PWN45_001084"
     CDS             18195..19556
                     /locus_tag="PWN45_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL0622863.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            19556..20308
                     /gene="pxpA"
                     /locus_tag="PWN45_001085"
     CDS             19556..20308
                     /gene="pxpA"
                     /locus_tag="PWN45_001085"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL0622864.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            20321..21550
                     /locus_tag="PWN45_001086"
     CDS             20321..21550
                     /locus_tag="PWN45_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL0622865.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            21839..23059
                     /locus_tag="PWN45_001087"
     CDS             21839..23059
                     /locus_tag="PWN45_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL0622866.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
BASE COUNT         8402 a         3159 c         4483 g         7154 t
ORIGIN      
        1 gtaaagttga aggggcgctt tcatatttgc attagaattt taagtgaatt tgaatcatta
       61 ctaaaataaa tgattgaaca ataaagacat ctttcattca aaatgctgtt actgtatatt
      121 tttattaata ttatggatac atactttcac ttagtttatt tatcacatta taaagtgttt
      181 ttttcattta tctgatttat aacattcttg aaccgacgat aagagtctaa ttgcatatct
      241 ttatcataaa tataacttat tgccataatt tcgttgactt caccataagt ttccataaaa
      301 tcttgaattt tttgttgaac agtattttct gaacctatta atgatatttt ctgtctttcc
      361 atagccatcg cgtattctct cggcgttaat atattttgta gttcatgtgt tggcggttga
      421 acgggctgca ttctgccacg agtgatacta accattactt gtgccataga tgaagctaaa
      481 tattgtgctt cgtcatcatt ttcagcaacg attgtattta aacatacaat aacataaggt
      541 tcgtctaata catcagaagg ttcaaataac gttttgtaaa tttcgatagc ttctttcatt
      601 tgttgaggtg caaaatgtcc agcgaacaca tatggaagcc ctttgcgagc agctaaatgt
      661 gcagaatctg ttgaagaacc aagaatgtat aaaggcacat ttttacctac tgctggataa
      721 gcacgaacat atgcttgttg gtgagcaggg ccgaaatatt gttgtaataa tgaaacctct
      781 tctggaaatt tataaacacc atcatgttga tctcgtctta atgcacttgc ggtcatcata
      841 tctgttccag gtgcacgtcc taatcctaaa tcgacacgat ttggaaataa tgttgccatc
      901 gtgccaaatt gttccgcaac gattaatgga gcatgattag gtaacatgat gcctccagaa
      961 cctacacgta tgtgtttcgt atgttctaac gtatgttgaa ttaataaagc agttgctgaa
     1021 cttactaggt tgggagcgtt atggtgttca gcaatccaat atctttcata tgataattcg
     1081 tctaaatgtt gtgcgagatt aaccatatca ttaattgctg tttgttcatc ttcaccttct
     1141 cgaataggga caaggtttaa tgctgaatat ttaatcgcca tttttatatc gtccttcctt
     1201 ttttatgata taagaagtat accaatcgaa taaccagata aatattaaat tgctcataac
     1261 caaaataata gtacgttaca ttttacaaat aaaaaaatat ctgcaaatac taaaatttat
     1321 aaaaaagaca agaataaagc gataaactta aaaaagccta tcgaaaagca tgtgtagaaa
     1381 atggtaaaat ataattaaaa acattataaa taaagttaaa cagaaaagta gaaaaatagt
     1441 atgtaaggtg ctattattgg aacattgata aaattgtgag gagtgtccaa acatggaaat
     1501 ttatgcagat tatgctgcga ccacaccagt aaaacctgaa gtagtagatg cgatgatgac
     1561 gatttacaat tcgcattatg gtaatccatc atcaatacat gctaagggaa gagatgcgcg
     1621 taaatattta gatgagtcaa gacgtcaaat tgcacaatta cttggtgcag atacacatga
     1681 aattatattt acgagtggcg caacagaatc gaataatacg gcaattaaag gcatagtaaa
     1741 agcaaatgaa caattgggta atcacattat cacatcgaag attgaacatc attctgtgtt
     1801 acatgttttt gaacaattag agcgagaagg ttttgatgta acatatctag atgttgatga
     1861 tacaggtgct attgatttag atcaacttga agaaacaatt acagataaga cgattttagt
     1921 atctattatg tttgtaaata atgaagttgg cacagtgcaa caaatttatg atattcaaga
     1981 tattattgct gagacaaatg catatttcca tgttgatgcg gttcaagcaa ttggacatct
     2041 tgatgttaag tttgacgaat ttgagatcga cgcaatgagt attactgctc ataagtttgg
     2101 cggaccaaaa ggtgtcggtg cattactagt taaagaccat gttacactag attatcctca
     2161 acttggcggt gaacaagaat tgaaacgccg tgcaggcact gaaaatcttg cgcaaattgt
     2221 tgggatggct aaagcattac aattagcaga aaagaatcga gacgataaca atattcattt
     2281 aatgaattta aaagaacaat ttttagttaa attacaagaa agagcgattc catttgaatt
     2341 aaatggttca atgacggatg ctacaggcca tattgtaaat ctatattttc cgtttgttga
     2401 agttgaaacg atgttaacat tgttggatat ggcacaaatc tatgtgtcgt ctggatctgc
     2461 atgtacagca ggctctacac aaccgtcgca tgttttagat gcaatgttcg aagatgagga
     2521 gcgatcaaat cattcgattc gatttagttt taacgaattg actactgaaa atgaaattaa
     2581 tgcaattgta gctgaaattc ataaaatata ttttaaattt aaggaggagt cataattgtc
     2641 aaataaagat atacgtgttg tcgttggtat gtcaggcggt gtagatagtt ctgtaacagc
     2701 ccacgtctta aaagaacaag gttatgatgt cattggcata tttatgaaaa actgggatga
     2761 cactgacgaa aatggcgtat gtactgcaac tgaagattac aacgatgtta ttgaagtgtg
     2821 taatcaaatt ggcattccgt attacgctgt taattttgaa aaagaatatt gggataaagt
     2881 ctttacgtat ttcttagatg aatacaaaaa aggtcgtact ccaaatccag acgttatgtg
     2941 taataaagaa attaagttta aagccttttt agatcatgcg atgaatttag gtgcagatta
     3001 tgtagcaaca ggacattacg cacgcataca tcgtcatgaa gatggtcatg ttgaaatgtt
     3061 acgtggtgta gataataata aagatcaaac atacttcttg aatcaattat ctcaacaaca
     3121 actttcaaaa gtgatgttcc caattggcga catcgaaaag agtgaagtgc gtcgaattgc
     3181 tgaagaacaa ggacttgtta ctgctaagaa aaaagattct acaggcattt gttttatcgg
     3241 cgaaaaaaac tttaaaacat ttttatcaca atatttacct gcacaaccgg gtgatatgat
     3301 aacacttgat ggtaagaaaa tgggtaaaca tagtggtttg atgtattaca caataggaca
     3361 aagacatgga ttaggtatag gtggagatgg cgatccttgg tttgttgtcg gtaaaaacct
     3421 aaaagataat gttttatatg tagaacaagg attccatcac gatgcattat acagtgatta
     3481 cttaattgct tcagactatt catttgtaaa tccagaagat aatgacttag atcaaggttt
     3541 tgaatgtaca gctaaattta gatatcgcca aaaagatacg aaagtttttg tgaaacgtga
     3601 aaacgaccat gcactacgtg ttacttttgc tgagccagta agagcaatca cacctggaca
     3661 agcagttgtt ttttatcaag gtgatgtgtg tcttggtggt gcaacaattg acgatgtgtt
     3721 caaaaatgaa ggtcaattaa attatgttgt ataaacaatg gcaacaataa attacttatt
     3781 tgaagtttca acgttgaaaa tgacgaaaga cagtttttga tgagaataat tcatgaggat
     3841 agagtctggg acatcacaat gtcctaggct ctacaatgtt atattggcgg gaccacaaca
     3901 tagagaattt cgtaaagaaa ttctacaggc aatgccagtt ggggataacg aatttaattt
     3961 tgttaaaata tcatttctgt cccactccct atgcatgaat ctaattatgt attcttattt
     4021 ttaagtacat aatagtggtg gctaatgtgg aagaaccatt acataataaa ccgttaatgg
     4081 ttcttaagca tttctattcc attcccgctt tttcatgaat gaagatgata ttagattata
     4141 ttttattcgt tgttaagtga ttcgagacat acaatttatc aagatgttat aattgatgag
     4201 aaatgaggtt cgtaaatgat agatcaacaa acaatttatc aatacataca aaatggaaaa
     4261 atagaagaag cgttacaagc attgttcgga aatatcgaag aaaatcctac aattattgaa
     4321 aattatatta atgctggtat cgtacttgct gatgcgaatg agattgaaaa ggcagagcgt
     4381 tttttccaaa aagctttaac aatagatccg aagaatggcg tcgtattttt taatctagca
     4441 aatgtatatt ataatcagca acgttatcaa gaagctatta aattatatca acaagcatta
     4501 caaacagaga ttgaacaagt tgattgtaat tatatgatcg gtatggcgtt taatcagtta
     4561 gaatcattta agctggcatt gccgtattta atgactgctg cggaactaga taaagacaaa
     4621 gatgcagaag ttcaatttca atatggtctt gtattatgtc aattagaaat gtttaatgaa
     4681 gccataactc aacttaaaca tgtattaacg attgataaaa atcatgttga tgcaagatac
     4741 aatttgggct tagcgttatt tatgaaaaat gaagatattg atgaagcaat aactcatttt
     4801 aaagaagctg tgactatcga ccctaaacac ttattaagtc agcatgcgct gaaaacattc
     4861 actaaaatga aagaggagga gtaacatgtc agaccctaca ctatttgatt attcaatgat
     4921 caaaggtact gttgaagcga tattgtttca aaacagtgat aatttttata cagtgcttaa
     4981 agtagatact attgaaacaa atgaagactt tgatacaatg ccaactgttg tagggtttct
     5041 tccgaatatt gttgaaggcg atgtctacac atttaaaggg caagttgttg atcatccacg
     5101 ttatggtaaa caattaaaag cagagacttt tgaaaaagaa atgccacaaa cgaaagaagc
     5161 aattataagt tatttatcta gtgatttgtt taaaggtgta ggtaaaaaga cagctcaaaa
     5221 tattgttaat acattaggtg acaatgcgat aaatgatata ttagatgacc attcagtgct
     5281 agaaaaagtt tcgggattat ctaaaaagaa acagaaacaa attgcagaac aaatttcagc
     5341 aaatcaagaa tctgaaaaaa taatgattcg tttacatgat ttagggtttg gtcctaaact
     5401 ttcaatggca atttatcagt tttatctcgg cgatacactg actattttag atcgaaatcc
     5461 atatcaattg atttatgata ttaaaggtat tggttttaat aaagcagatc aacttgctag
     5521 aaatattggt atcgcttata acgataatga acgattaaaa gctgcgttgc tctatacgtt
     5581 agaagaagaa tgtattaaac agggacatac atatttgcct atcaatgttg ttatagattt
     5641 aacggttgat gtattaaatt atcaagatga agaggtcatc gaacctgaaa aattagacga
     5701 aatgttgcaa tacttaaatg aggaaaaacg actaattatt gacaatgaac aagttgcaat
     5761 tccaagtttg tattattctg aaatcaaaag tgttcaaaac ttattcagaa taaagacgca
     5821 tacaaataaa cttacagaaa ttgaacagtc agatttacaa atgcatattg gtgaaataga
     5881 agatgcgaat caagtgaact atgcagcttc acaaaaagaa gctttgcaaa cggctatcaa
     5941 ttctaaggtg atgctattaa ccggtggacc gggaacagga aaaacgacag taattaaagg
     6001 tattgttgaa ttatatgctg agattcatgg cttatcatta gattatgatg attatgtcaa
     6061 tgatgattat ccagttgtat tagcagcgcc aacaggaaga gcgtctaaga gactacaaga
     6121 gtcaactgga ttagaagcaa tgacgattca tcgtttaata ggttggaatc aagacacaaa
     6181 gccagaggat atattagaaa atgaaataaa cgcacgactc ataatcattg atgagatgtc
     6241 tatggtagat acttggttgt tccaccaatt tttaagtgca gttcctttag atgcacaatt
     6301 gatatttgta ggtgatgagg accaattgcc ttctgtaggt cctggtcaag tatttaaaga
     6361 tttaattgaa tcgaaagcta taccacgagt aaatctgact gaagtatatc gtcaacaaga
     6421 tggttcaagc attatagaat tagcacatcg aatgaagttg ggtcagaaaa ttgatattac
     6481 acagcgtttt catgatagaa gttttattaa ctgtcaggca aaccaaatac caacagttgt
     6541 tgagaaggta gttactagtg ctgttaataa agggtatact atggctgata ttcaagtgct
     6601 tgcaccaatg tataaaggaa atgccggtat taaacgttta aatcaagttt tacaagatat
     6661 tttaaaccct aaaaagaaag acacacgaga aattgagttt ggtgatgtag tctttagaaa
     6721 aggtgacaaa gtattacaac ttgttaatag gccaaatgat aacatattca atggtgacat
     6781 aggagttatt gttggtattt tttgggcgaa agaaaatgcc cttaataaag atgtacttgt
     6841 tgtggatttt gaaggtaatg aaattacatt tacgaaacaa gatatgatgg aattaacaca
     6901 tgcttattgt acatcaatcc ataaatcaca aggttcagaa tttcctattg ttatcatgcc
     6961 tattgttaaa cagtatttta gaatgttaca aagaccgata ttatatactg gtttaactag
     7021 agcgaaaacg agtttagttt tacttggtga tccggaagca tttgatattg gattgaaaac
     7081 aaatggtcaa gcgcgtttaa cacaattatg tacattgttg aaaaattatt ttaattcaga
     7141 agatgaagaa atgctggaaa acacagcaac taatgataca ggagcttcac aaacgacaat
     7201 agatgatcaa gtagaagcgc caatgtcatc gaatgatagt gaagaagtta tgtctgattc
     7261 aacaaaaaca gatacaaatg tattaacgga agctacaatg tttaaaattg acccaatgat
     7321 taatatggga gaaatcacgc cgtatgactt tatagaacgt tgacaacaag cgtaaatgta
     7381 actaaaatag aattaaattc gtataaagat gagtagttaa tgatttgttt atcagagatg
     7441 tactggttgg tgaaaagtac aacaaacatt agtgaacgct actttatgca caactgtata
     7501 gtcgaattaa agtgataaat gtgttgaatt gcatttataa ctagggtggt accgcgataa
     7561 cgttcgtccc ttttgaggat gggcgttttt tattttttaa taataaaaaa tgatattgat
     7621 ttatactagt tttgcaaatc gactatctgt gtcatcaatt tttatggaat ggtaatttaa
     7681 aaacagtaaa taggatgtgt aaatgatgaa aaagttaaaa gcgagtgaaa ttagacaaaa
     7741 atatctagat ttctttgttg aaaaaggaca tatggttgaa ccttctgcac cattagtgcc
     7801 aattgatgat gatacattat tatggattaa ttcaggtgta gcaacattaa agaaatattt
     7861 tgatggacgt gaaacaccta aaaagccaag aattgtaaac tctcaaaaag ctattcgtac
     7921 aaatgatatt gaaaatgttg gtttcacagc gcgtcaccat acattctttg aaatgttagg
     7981 taacttctca attggtgatt attttaaaca agaagcgatt gaatttgctt gggaattttt
     8041 aacgagtgat aaatggatgg gtatggagcc agataaattg tacgttacga ttcatccgga
     8101 agatatggaa gcatacaaca tttggcataa agatattggg cttgaagaaa gtcgtattat
     8161 tcgcattgaa ggtaacttct gggatattgg tgaagggcct tcaggaccga acactgagat
     8221 tttctatgat cgcggagaag catatggaca agacgatccg gcagaagaaa tgtatccagg
     8281 tggagaaaat gaacgctatc ttgaagtatg gaacttagta tttagtgaat tcaatcataa
     8341 taaagatcat agttacacac cattacctaa taaaaatatt gatactggca tggggcttga
     8401 gcgtatggcc tcagtttctc aaaatgtacg tactaactat gaaacagatt tatttatgcc
     8461 tataatgaat gaaatcgaaa aagtatcagg taaacaatat ttagtaaaca acgaacaaga
     8521 tgtggcattt aaagtaattg ctgaccacat tcgtacgatt gcatttgcaa tttctgatgg
     8581 tgcattacct gccaatgaag gtagagggta tgtattacgt cgattgttac gtcgtgccgt
     8641 tcgttttagt caaacgttag gaatcaatga gccatttatg tacaaacttg ttgatattgt
     8701 tgcagacatt atggaaccat attatccaaa tgttaaggaa aaagcagatt tcattaagcg
     8761 tgttataaag tctgaagaag aacgattcca tgaaacatta gaagatggtt tagcgatttt
     8821 aaatgaatta attaaaaaag ctaaagcgac aacaaatgaa attaatggga aagatgcatt
     8881 taaattgtat gatacgtatg ggttcccaat tgaattaact gaagaaatag cagtgcaagc
     8941 aggattgaaa gttgatatga caacattcga gtcagaaatg caacaacaac gtgatcgtgc
     9001 acgtcaagca cgtcaaaatt ctcaatcaat gcaagttcaa agtgaagtat tgaaaaatat
     9061 tacatctgca agtacttttg ttggttatga tactgcgaca gctcaaacaa cactaacaca
     9121 cttgatatac aatggtgaag aagtttcaca agttgaagcg ggtgaaacag tatacttcat
     9181 gttaacggaa acaccatttt atgcaatcag tggtggacaa gttgcggata caggtattgt
     9241 ttataatgac aattttgaaa ttgctgttag tgaagtaacc aaagcaccaa atggtcaaaa
     9301 cttgcataaa ggagtagtac aatttggcca agtaaatgtt ggcgctacag tgtctgctga
     9361 agtgaaccaa aatgatcgac gtgacattca aaagaaccat agtgcaacac atttattaca
     9421 tgcagcgttg aaatcagtac tgggtgatca tgttaaccaa gctggttcac tagtagaagc
     9481 agatcgttta cgttttgatt tctctcattt tggtccaatg actaatgatg aaattgatca
     9541 agttgaacgc ttagtaaatg aagaaatttg gaaaggtatt gacgttaaca ttcaagaaat
     9601 ggatattgct tcagctaaag aaatgggcgc aatggcatta ttcggtgaaa aatatggtga
     9661 tgttgtgcgt gtagtaaata tggcaccatt ttcaattgaa ttatgtggtg gtattcatgt
     9721 ccgcaatact tctgaaattg gcttattcaa aatagtaagt gagtcaggta caggagctgg
     9781 tgtgcgtcgt attgaagcat taacaggtaa agcagctttc ttatatttag aagatattca
     9841 agagaaattt aatacgatga aatcacagct gaaagtgaaa tctgatgatc aagtagtcga
     9901 taagttaaca caattacaag atgaagaaaa agcattatta aaacaattag agcaacgtga
     9961 caaagaaatc acatcactta aaatgggtaa tattgaagat caagttgaag aaatcaatgg
    10021 ctataaagta ttggttactg aagtggatgt accaaatgcg aaagcaattc gctcgacaat
    10081 ggacgatttt aaatctaaac tacaagatac aattatcatt cttgcaagta atgttgatga
    10141 taaagtatcg atggttgcaa ctgtccctaa atctttaaca aataacgtta aagccggtga
    10201 tcttatcaaa caaatggcac caatcgttgg tggtaaaggt ggcggtcgtc cagatatggc
    10261 tcaaggtggc ggtacacaac ctgaaaatat ctcaaaatca ttaagcttta ttaaagatta
    10321 cattaaaaat ctataattta aagccataat attgtatgat gatagtgagt tatatttgta
    10381 attaaaggag tgtcgcaaat ggaaaacttt gataaaacaa tgaaattcga ctatgaagaa
    10441 cttccaacgc aagatgtaag agatgtttta aataatgttt atcgaacatt agatgaacga
    10501 ggatataatg ccgtaaacca aattgtaggt tatttattat caggtgaccc tgcgtatatt
    10561 ccacgccaaa atgaagcaag aaatcaaatt cgtcatattg atagagatgt tattatggaa
    10621 gagcttgttt cttactattt aaaagagcaa aataaataat ttatgttaca acataaaatt
    10681 ttaggactcg atgtcggtag tagaacggta ggaattgcaa ttagtgatat aatgggttgg
    10741 acggcacaag gattagacac actccgaatc aatgaagaaa ataatgaatt aggtattgac
    10801 caattagtag acattattaa aaaacataat gtgggtacag tcgtaatagg actacctaaa
    10861 aacatgaata attcaatagg atttcgaggc gaagcttcgt taacatataa agaaaaatta
    10921 ttagaagctt atccttctat tgagattgtg atgtgggatg aaagattaag cacaatggct
    10981 gctgaacgat cattattaga agcagatgtt tcaagacaaa aaagaaaaca agtgattgat
    11041 aaaatggcag cggtatttat tttacaaggc tatttagatt cactacatta aaaggagatt
    11101 ttactatgac tgaacataat catgattcac aactagaaat taataacgaa gaagaattat
    11161 taactttatt cgatgaagag ggaaatgaag ttttataccg aaaagtttta gaattttatc
    11221 atcctgaatt caaaaaagag tatgttatct tagctgaaga aggtgctcaa tcagatgaag
    11281 acgatatgat tgagcttgta ccaatgatca atgaaccaga tgagtcaggt gacggtggta
    11341 agttagtacc aatcgaaact gatgaagaat gggacatgat tgaagaagtt gtaaatactg
    11401 aaatggaaga ataattaaaa agattttact cttgctaacc ttaaagatgg ggaggcaaga
    11461 gtatttttta agtcaagcaa tgtataaata attaacgctt ataattttat tgaaaatatg
    11521 gtaaaatatc taaagttaaa ttttatatga atgattcgct tctataaata aaatagcttt
    11581 gcgtctaaca cgaaatcagt catttaattg gttggcaaaa ttataatcaa ttgtgaaggc
    11641 tgatttattt tattgtcata atcatattga cttattttac ataaaggaag tttaaaacat
    11701 ggatgaccta aataaaaaat atttaataga tttacatcaa catcaaaata gttcaatcga
    11761 agttttgcgt gaatttgccg aggtaaatga agtgccaatt gtagatcgtt taacattaga
    11821 tttaattaag caattaattc gtatgaataa tgttaaaaat attttagaaa ttggtacagc
    11881 aatcggctat agttctatgc aattcgcttc tatatctgat gacattcatg tcacaacgat
    11941 agagcgtaat gaaacgatga ttcaatatgc taaacaaaat ttagctactt atcattttga
    12001 aaaccaggtt cgaattattg aaggtaatgc tttagagcaa tttgaaaatg taaatgacaa
    12061 agtttatgat atgatattta ttgatgcagc aaaagcgcaa tcaaagaaat tttttgaaat
    12121 atatacacca cttttaaagc accaaggtct cgtaattaca gataatgttt tatatcacgg
    12181 ttttgtatcg gatattggga ttgttagatc gagaaatgta agacaaatgg ttaaaaaggt
    12241 tcaagattat aatgagtggt taataaagca accaggatat acaacgaatt ttttaaatat
    12301 agacgatgga ttagcgattt caattaaagg agaatgacta tgacagaatt acttgtcaca
    12361 cccaagtcat taagccatat ggaaacactt attgatttag gtgcagatgc ttttgtaatt
    12421 ggcgagcaaa agtttggttt aagacttcca ggggaattca atagacaaca aatgacagag
    12481 gctgtcgcat tagctcacaa aaacgataaa aaagtatatg cggcagtgaa tggattattt
    12541 cataattatc atttagatgc tgtagaagat tatattaatt ttttacatga aatacgagtt
    12601 gacagaatta ttttcgggga tccagcagta gtaatgtatg tgaaggcgca agaaaatcca
    12661 ataccacttc attggaatgc agagacgtta gtaactaatc attttcaatg taattactgg
    12721 ggtaaaagag gcgcatcaag agctgtctta gcacgtgagc tgaatttgga tgaaattatt
    12781 aatataaaag aaaattcaaa tgtggaaatt gaggtgcaag ttcaaggaat gacttgtatg
    12841 tttcaatcaa agcgtatgct actaggcaat tattatacgt tccaagatcg ccaaatgaaa
    12901 attgaacgtc gcaatgatga acaatcttta ttactttatg atgaagaaag acaaaataat
    12961 tacccagttt acgaagatta caatggtacg cacattatgt caccaaacga catttgttta
    13021 attgaagaat tagcaccatt ttttgaggcg ggtatagatt catttaaaat cgatggtatt
    13081 ctacaaacgg aagaatatat taatgtggta acggaacagt atcgtcaagc gatagatttg
    13141 tacaatgaag atcctgaaat ctatgaagat gagaaattta tgttgatgga tccaattgaa
    13201 gaaattcaac ctgatcatcg accatttgac gaaggtttct tatataaaca aacagtatat
    13261 taaggaggtt aatcatgaag acaatagaag agattaaatc aactcctaaa acagttatga
    13321 agaaaccaga attattagca cctgctggaa acttagaaaa gttaaaaata gcagtacatt
    13381 atggcgctga tgccgtattt ttaggtggtc aagaatatgg attacgttca aatgctgata
    13441 atttcacgat ggaagaaata gctgaaggtg ttgaatttgc gaaccgttac ggtgccaaaa
    13501 tttatgttac gacaaatatt attgcacatg atgagaatat tgaaggtcta gaatcatatt
    13561 tgcgtaattt ggaaaagact ggtgcgacag gtatcattgt tgcagatcct ttaattatcg
    13621 aaacatgtaa agaagttgcg ccaaaacttg aaattcattt atctactcaa caatcacttt
    13681 ctaattacaa agctgtagaa tattggaaag aagaaggatt ggatcgtgtt gtattagcac
    13741 gtgagaccgg cgcgatggaa atgcgtgaaa tgaaggaaaa agtagatatt gaaatcgaag
    13801 catttattca tggtgctatg tgtatcgcct attcaggtag atgtacatta agtaatcata
    13861 tgactgcaag ggattccaac agaggcggtt gctgtcaaag ttgccgttgg gattatgaat
    13921 tattagaagt tgatgataat ggtgaacttg atgtttttta taatcaaggt gaagttacac
    13981 cgtttgcgat gagtcctaaa gatttaaaat taatcgaatc aattcctcaa atgatggata
    14041 ttggtgtgga ctcattaaaa attgaaggac gtatgaagtc aattcattat attgcaacag
    14101 ttgtctcagt atatcgtaaa gtcattgatg cgtatgcggc agatcctgac aactttaaga
    14161 ttaatccgga atggttaata gagttagata aatgtgcaaa tagagacact gcaccagcat
    14221 tctttgaggg aacacctggt tatgaagaac agatgtttgg tcaacaacaa tctaaaaaat
    14281 caccatttga tttttgtggt ttggtattag actataatga agatacaaaa attgcgacta
    14341 ttcaacaacg aaataacttt aaaccaggtc aagaaataga attctttggt ccagaaattg
    14401 aaacatttac acaagtagta gaagcaattt atgatgaaga aggtaacagc ttagacgcgg
    14461 cgcgccatcc attacaaatc gttcaaatta aagtggatcg cccgatatat ccaaacaaca
    14521 tgatgagaaa ggaaattggc taatgaaagc tactacaatc attggcatag ctggtggatc
    14581 tggctcagga aaaacaactg taactaacga aattatgaaa aacttagaag gtcatagtgt
    14641 cgctttactt gctcaagatt actattataa agatcaaaag cacttgactt tcgacgagcg
    14701 cctagaaacc aattatgacc atccatttgc attcgataat gatttattaa ttgaaaatct
    14761 taaagacttg aaaaatggta aagcagtaga agtaccgaca tatgattatg ctagtcatac
    14821 aagaagtgac attaccattg attttaaacc taaagatgtt attatcgtag aaggcatttt
    14881 cgctttagaa aataaggtat tacgtgatat gatggatgtt aaaatatatg ttgatacaga
    14941 tgcagacttg agaatattac gccgtttaac acgagatact aaagagcgtg ggcgttcaat
    15001 ggactctgtt atcaatcaat atttaagtgt tgttagacct atgcatgacc aatttattga
    15061 accgactaag aaatatgctg atataattat tcctgaaggt gggagcaata aagttgcaat
    15121 agatattatg acaacaaaaa ttcagtcttt agttagcaag caatagtata attcagtaaa
    15181 ggaagatgac aatatggaaa atcaaaagca atatccaatg actcaagaag gttttgaaaa
    15241 attagagcgt gaacttgaag aattaaaaac agttaagcgt cctgaagttg tagagaaaat
    15301 taaagttgca cgttcatttg gtgacttatc agagaactct gagtatgatg cagcaaaaga
    15361 tgaacaagga ttcatcgaac aagatattca aagaattgag catatgttaa gaaatgcatt
    15421 aatcattgaa gatactggag ataacaacgt tgttaaaatt ggtaaaacag taacgtttgt
    15481 agaattacca ggtgatgaag aggaaagtta tcaaatcgtt ggttcagctg aatcagatgc
    15541 atttaatggt aagatttcaa atgaatcacc aatggctaaa gcgttaattg gtaaaggttt
    15601 agatgatgaa gttcgtgttc cactacctaa tggtggcgaa atgaacgtaa aaattgttaa
    15661 tatccaataa atgatcaaat agattattta attgcgcgat tgaagtgtgt taatgatact
    15721 tcggtcgtgc ttttttatat tcaaaatcaa aaaatcggac agatgaaaag taaaaatttt
    15781 ccatcagacc gaatgatagc agaacctaaa ttagtgtaag agttacaata ttaactactc
    15841 cattttaaaa aagcaaaata tttccgagaa aacaaattga ttatcatttg ttattgtatg
    15901 cgatttgaat tacttattaa acaacttgtg cttctcaatg atacaatgaa aaggtattca
    15961 atactggttt attgcaatac aaagggggat ttaaagtgga tgtaagattt attaacgagc
    16021 agacaattat gatttatttt gaaaataaaa tatccgaaga gacatatcgt aatgtcacag
    16081 caatggtacg gtggatacga gaaaaagaaa tattagaaat tcaagatatt gtcccttcgt
    16141 atcgagcagt tctaatatat tttgatgaac aagctataac atcttctaaa ttaattgaaa
    16201 atttagaact taacaaattt aatgaaaaaa atgttcatgc tgtgaatcaa accaatcgta
    16261 ttattaaaat acctgttcaa tatggtggta catatggacc agatattgaa gaagtagcaa
    16321 agcataatcg aataactgtt gagcaagtta ttgaaaaaca tacaagtaaa ccttatttaa
    16381 tatatatgct aggatttatg ccaggatttc catacttagg cggacttgat gaacaactac
    16441 atacacctag acgtaatcag ccaaggttaa aaattcatgc aggttctgta ggtattgcga
    16501 ataatcaaac aggactatat ccatcagatt ctcctggcgg atggcaaatt attggacgca
    16561 caccgttaaa agtatttagc agcgagcgtg aaccaatgtc aatgtacgaa gcaggagaat
    16621 ggatacaatt ttatgcaata gatgaacaga aatttataca aatcgaaaga gatatatcag
    16681 atggcaactt taatgttgat gactgggtgg tgattgaaaa tgtcaattaa gattttacaa
    16741 ccaggtctct tttcaacggt acaagatcta ggaagaatag gttatgaaca tattggattt
    16801 tcaggcgcag gtgccatgga tcaatttagt tttaaggttg cgcagtcatt aattaacaat
    16861 gatggtccag cgattgaata tactttgatt ggtcctacca ttcaatttaa tacgcaaaat
    16921 acatttgtta taaccggtgg tagtattaat gcttcgctaa ataataaaac tatatcaatg
    16981 aattctgtca tattagctga gaaaggtgac attttaaaaa taggtgctat aaccaaaggt
    17041 gcacgcggtt atcttacttt tggtcattct atcaacgtac cttctattgc tgaaagttat
    17101 gcaacacata cccgaagcag tataggagga ttcaaaggta gaaagttatt agctgatgat
    17161 gtaataacag tgaaaataaa taatgacttt aaagaaaaca ttggaaagac gattcattta
    17221 caggacgatt tattgccaga aaataatatt atacacattc ttcaaggacc tcaattcgag
    17281 gcattttctg aagaggctag agcgaaaatt gtaaatcatc catatttaat tactgaacaa
    17341 tcagaccgta tgggctatcg tttggaaggt gacagcgttg caccaattaa tcaagcagat
    17401 atcatttctg aaccggttgc tcttggtagt gttcaggtac caaatgatgg tcaacctatt
    17461 attctgctta atgataaaca aacgataggt ggttatacca aaattgcaac tgtatgcaaa
    17521 tttgatctgc caaagttagc acagatgaaa ccacaagata caattcaatt taaatggata
    17581 tcattccaag aagctgtaga taagaatcgg gaacaaatgt ctctgtttaa tgaaattctt
    17641 aagagtcatc aaaagactcc aatttttgat acatctagct tgcgacatac ttcgaaaaaa
    17701 ttagcaacaa ttttaaaagg ggatttgtaa aatgaatatt gaaaaaatcg aacaaataat
    17761 caaattagtg aaggaaaatg atgttaagaa atttaaatat aaaaattttg aagatgaaat
    17821 agaaattgac ttcactgact cgaatcattt ggctgcacac agtaatcaat caaatcaaag
    17881 tatgaacaat aatgatttga cagcttcaaa agcgaatgat aactccgatg tttcgacaaa
    17941 tgattatcat gacattaaat caccaatgat aggtacattc tttttacaag atagtaaaga
    18001 attaactgaa ccaattgtga atgtcggtga caaagttaac aagggagata ttataggata
    18061 tgttgaagcg atgaaagtat taaacgaggt aacaacagat gttgctggag aaattactga
    18121 aatagtagct gatcatggca caaatgttga atacgaccaa gttttggtac gtattaagta
    18181 aagggggtaa gtaaatgctt cgttgtttaa ttgcgaacag aggtgaaatt gctgtaagga
    18241 ttataagagc ttgcagagaa tatgggattg aaactgtagc agtttatgca aaaggggacg
    18301 agcaaagctt gcatgtacat ttagcagatc aagctatatg tattggggaa gctaatgctt
    18361 tagatagtta tttaaatatt gaccgcatca tatctgctgc acaaatcact ggtgctaatg
    18421 caattcaccc aggatatggc tttttatcag aatcaacaaa atttgctcaa accgttgagg
    18481 aacaaggcat agcgtttata gggcctacta aaaaaacgat ggaaatgatg ggggataaaa
    18541 taacagcaag acaaacagtt catcatgcag gtgttcctgt gattccagga tcaaatggtg
    18601 ctgtgaatca tgtgtctgaa attgagaatc ttgccaaaga catcggttat ccagttgtta
    18661 tcaaagctgc cagtggcggt ggtggtaaag gtatacgtat tgtaaagaaa gctgaagatt
    18721 tagaaaaggc atttaaagaa gctaaaagtg aaggaaaaaa atactttgat gatgatcgtg
    18781 tttatgttga ggcttttata cctgttgcaa aacatgttga agttcaagtt atgggagatg
    18841 ggcaggataa ttatgtacat ttaggggaac gtgattgttc tgtacaacgt aaaaatcaaa
    18901 aattaatcga agaatctcct tgtgcagcat taactgaaga aagacgacaa caaatatgta
    18961 acgatgctgt taaagttgca cgtgcagcaa attatagaag tgcaggtacg attgaatttt
    19021 tagtaactga taccgcacat tactttattg aaatgaatgc tcgaatacaa gttgaacata
    19081 ctgttacaga gatgcgagca gaacgtgatt tggttgcagc acaactgtat ttattggaac
    19141 ataatcattt acctttttca caaagcgata ttcaatttaa tggtcatgtg atagaagcga
    19201 gaataaatgc cgaaaatcct gaaaagaaat ttcaaccaac accaggtaaa gtgacagcgt
    19261 tacatttacc tcaaggcttt aatgtgcgag tcgattcttt actatatcac ggttatcaag
    19321 tttcaccata ttatgattct ttagtagcta aagtaattgt aaaatctcat gatagagctt
    19381 cagcaattga taagttgaaa gtcaccttag atgaaatggt gatagatgga ttttctacaa
    19441 ctgccgattt tttatatgca gttttaaatt atccactata tcgtgatgga gatgcaaaag
    19501 atgttgatat taaattttta gaaaaacatc aaattgttaa aggggtggaa ttataatgcg
    19561 agttgatttg aattgtgatt taggcgaagc atttggaaat tattcctttg gtggtgatca
    19621 tcaaattatt ccgttaatta caagtgcgaa tgttgcttgt ggttttcacg ctggtgatga
    19681 aaatgtaatg aatgaaacgg taaaacttgc caaagcacat aatgttgcag taggtgcaca
    19741 tcctggttta cctgatttga aaggctttgg cagacgaaat atagatatct ctaacgacga
    19801 gatttataat ttgatgattt atcaattagg tgcattacaa gggttttgtc gcattcatca
    19861 agttaaaatt aatcatgtta aaccgcatgg tgcattgtat cagatgggtg caaaagacag
    19921 agaaatagca aacgttatag cacaagctgt ttatgacttt gatccatcac tagtgttagt
    19981 aggattagca aattcatatc taatttcaga agcaaagaat gtcggattaa ttacagcttc
    20041 tgaagtgttt gctgatagac gatacgaaga tgatgggcag ctcgttagta gaaaagaaag
    20101 tgatgctgtg attactgata ctgacgaagc acttaagcag gttttaaaga tggtgaagga
    20161 aaataaagtt atttcaaaaa acaataagga agtaacgtta caagcagata caatttgtgt
    20221 gcatggtgat ggagaacatg cattattatt tgtttcgaaa attagagaaa ttttaatgaa
    20281 agaaggcatt gatattcaat ccttataggg ggctaaaaaa atggggagaa atttaaagct
    20341 taaaaaagaa agtgactttg aattcacaaa aaatcataaa aggttattat taggttctgt
    20401 atttttgatg gcaacttctg caattgggcc agcattttta acgcaaacag cagtatttac
    20461 atcacaattt tttgcaagtt tcgcatttgc catattactg tctatcatca ttgacattgg
    20521 tgcacaaatt aatatatggc gcatattagt tgtaactggt ttaagaggtc aagaaatatc
    20581 aaataaagtt gttcctgggc ttggtactgt tatctcaata ttgattgcat ttggtggtct
    20641 agcttttaat attggtaata ttgctggtgc cggtttaggt ttaaatgcaa tttttggatt
    20701 agatgtaaaa tggggcgcag ctattactgc aatctttgca atattaatct ttgtaagtaa
    20761 aagtggccaa aaaattatgg acgttgtttc aatgattctt ggtattgtga tgattttagt
    20821 tgtggcatat gtgatgtttg tttctaatcc accttatggt gatgcttttg tgcatacatt
    20881 tgcgccagaa catccaatga aattagtctt gcccatcatt acgttagttg gtggaactgt
    20941 aggtggttat attacctttg caggtgcaca tcgtatatta gactctggca ttaaaggtaa
    21001 gcaatattta ccatttgtaa atcaatcagc aattgctggt attttaacta caggtattat
    21061 gagaacgtta ctattcctag cggtattagg agttgttgta acaggtgtga cactaagttc
    21121 tgaaaatcca ccagcgtcag tttttgaaca cgcaattgga ccaattggaa agaatatttt
    21181 tggtattgtg ttatttgctg cagctatgtc atcagtaatt ggctcagcat acacaagcgc
    21241 aacattttta aaaacacttc ataaatcact taacgaaaga agtaatttaa ttgtgattgt
    21301 gtttatcgtt atttcaacaa tgattttctt atttattgga aaaccaatca gccttttaat
    21361 tatagcaggc gcgataaatg gatggatttt acctattacg ttaggtgcaa ttttgattgc
    21421 aagtaaaaag aaatcaattg ttggtgatta taaacaccca aattggatgt tcatctttgg
    21481 tatcgttgca gtacttgtta caatattgac aggtatattc tcattcaaag aagtacttca
    21541 gttattttaa aagacatttc gactaatagc atcactgtaa atgttgttag tattaggcaa
    21601 gatatatttt gatttccggt gattcattta taaagcataa ttagatatat tttaagggag
    21661 agtggtgcat gacaaatgta ctgctctttt tgttgtgatg aaaaatcttt ttctattgat
    21721 tgagaaaata gttatttata tagtttttaa agaaaaaaag tgataatatt tagctaatct
    21781 aatgaaaatt gatttattgg acaaaaaata cattttaaat gagtagagga ggctgcaaat
    21841 gggttacaga actttaaaaa gtatttttca tgaacacaat gaaagtaaaa tgaaggaaga
    21901 gtatattaaa agatttaatt ctttagcttc tttcaatact aatattaata tcatacctat
    21961 ggaaaatgga aaaaaagtta atgatttgga atatcctcta ttctttatgg tgactaaaaa
    22021 tctatcaaaa aaacaagaat taatatcaat taatagtaga aaaattgata gagcacttaa
    22081 ttctttacca tatgcagcta gagaacaata ttttaatgat ttattaatcg atgaattaca
    22141 aagtactaat gaaattgaaa atgtatttag tactaaacaa gagattgcac atgcgttaaa
    22201 taaccaagca tcagaatttc ttaagttcag aggcctcgtg gatcaatata aagagataga
    22261 acttaataaa aaaattaaag ttgataatgt aagagacatt agagcgattt atgataaatt
    22321 agtttcaaat gaaattaacg aacaagataa gttagatgga gagctatttc gtaaaaattt
    22381 tgtcggtgtg catgatgggt caacgaataa atatatacat gttgggttac aacctgaaac
    22441 caaaattgtt gaatttatag gtgaaatgct aacattttta aaatattttg atgcgcctca
    22501 gccgttcaaa atcatggcta gtcattatct gtttgaatat atacatcctt tttatgatgg
    22561 caatggtaga gttggaagat ttataattgc caagctcttg agtgattatt atgataatta
    22621 tactgctttg acattttcat atgttataaa tagaaataaa tcgaaatatt acaaggcgtt
    22681 tatgactgct tcaaatcatt tgaattgtgg tgatttgacg gaatttattg acacaatgct
    22741 tgagctattg atagcaggac aagaaagaat acttgatgag ttaataccaa aaatggatgc
    22801 gactgaaaaa ttaacgcttt atttaactag tcattataaa cagatagatt atgaattttt
    22861 gtatctcctt tcaatggata aattgtttgg aaataaaaga aatagattaa cattaattga
    22921 tttagaaaac atattgggag taggaagagt aaaaattaat aatacaatta aaaaatatga
    22981 taactactta gttaaaatta aaagtagacc aacaatttat gagataagtg atgaatttct
    23041 aaattcgatt ataaaataaa tataatatgt gatcagctat acttatagta tttgtaagat
    23101 cgcgtaattg cttcattgaa aaaatatatt tgttgagagt ggtgcatgac aaatgtactg
    23161 ctctttttgt tgtgtatcag tattgtgatg ttttaatg
//
LOCUS       DANJFZ010000031        23036 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.031, whole genome shotgun sequence.
ACCESSION   DANJFZ010000031 DANJFZ010000000
VERSION     DANJFZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.031"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(66..176)
                     /locus_tag="PWN45_001088"
     CDS             complement(66..176)
                     /locus_tag="PWN45_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622867.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(262..993)
                     /locus_tag="PWN45_001089"
     CDS             complement(262..993)
                     /locus_tag="PWN45_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL0622868.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(995..2308)
                     /gene="murT"
                     /locus_tag="PWN45_001090"
     CDS             complement(995..2308)
                     /gene="murT"
                     /locus_tag="PWN45_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDL0622869.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            2600..3100
                     /gene="ftnA"
                     /locus_tag="PWN45_001091"
     CDS             2600..3100
                     /gene="ftnA"
                     /locus_tag="PWN45_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDL0622870.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3182..3274)
                     /locus_tag="PWN45_001092"
     CDS             complement(3182..3274)
                     /locus_tag="PWN45_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622871.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            3489..4043
                     /locus_tag="PWN45_001093"
     CDS             3489..4043
                     /locus_tag="PWN45_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDL0622872.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4111..5181)
                     /gene="dinB"
                     /locus_tag="PWN45_001094"
     CDS             complement(4111..5181)
                     /gene="dinB"
                     /locus_tag="PWN45_001094"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL0622873.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5428..5958)
                     /locus_tag="PWN45_001095"
     CDS             complement(5428..5958)
                     /locus_tag="PWN45_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDL0622874.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6128..7489)
                     /gene="rlmD"
                     /locus_tag="PWN45_001096"
     CDS             complement(6128..7489)
                     /gene="rlmD"
                     /locus_tag="PWN45_001096"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0622875.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(7570..8517)
                     /locus_tag="PWN45_001097"
     CDS             complement(7570..8517)
                     /locus_tag="PWN45_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0622876.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(9100..9246)
                     /locus_tag="PWN45_001098"
     CDS             complement(9100..9246)
                     /locus_tag="PWN45_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622877.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9286..10713)
                     /gene="gatB"
                     /locus_tag="PWN45_001099"
     CDS             complement(9286..10713)
                     /gene="gatB"
                     /locus_tag="PWN45_001099"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL0622878.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(10726..12183)
                     /gene="gatA"
                     /locus_tag="PWN45_001100"
     CDS             complement(10726..12183)
                     /gene="gatA"
                     /locus_tag="PWN45_001100"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL0622879.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12185..12487)
                     /gene="gatC"
                     /locus_tag="PWN45_001101"
     CDS             complement(12185..12487)
                     /gene="gatC"
                     /locus_tag="PWN45_001101"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL0622880.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            12854..14392
                     /gene="putP"
                     /locus_tag="PWN45_001102"
     CDS             12854..14392
                     /gene="putP"
                     /locus_tag="PWN45_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL0622881.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            complement(14481..15680)
                     /locus_tag="PWN45_001103"
     CDS             complement(14481..15680)
                     /locus_tag="PWN45_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDL0622882.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(15693..17696)
                     /gene="ligA"
                     /locus_tag="PWN45_001104"
     CDS             complement(15693..17696)
                     /gene="ligA"
                     /locus_tag="PWN45_001104"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL0622883.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            complement(17700..19892)
                     /gene="pcrA"
                     /locus_tag="PWN45_001105"
     CDS             complement(17700..19892)
                     /gene="pcrA"
                     /locus_tag="PWN45_001105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL0622884.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            complement(19889..20581)
                     /locus_tag="PWN45_001106"
     CDS             complement(19889..20581)
                     /locus_tag="PWN45_001106"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDL0622885.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            complement(20753..21055)
                     /locus_tag="PWN45_001107"
     CDS             complement(20753..21055)
                     /locus_tag="PWN45_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622886.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            complement(21164..22459)
                     /gene="purB"
                     /locus_tag="PWN45_001108"
     CDS             complement(21164..22459)
                     /gene="purB"
                     /locus_tag="PWN45_001108"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL0622887.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
BASE COUNT         6986 a         4255 c         3273 g         8522 t
ORIGIN      
        1 aagatgcatc ttttaatatc actttagtag gcttcatcgt caaattcctt tcatcattca
       61 ttttttcatg ttgcataatc accattacta ttgataattc taggatagat actcattgta
      121 taataattat tcactagaac aaaaaaacac acatcaattt ggttgtttcc atccaaaata
      181 atgtgtgtgt ttatacatat atgattaatt actaaacttc atcgtttatc aatattaaat
      241 ccattgatgc gatgttcaga gttaacgaga tttcttctgt ctatttgctc tgtctattaa
      301 tacttgtttc gcttgtattt ccgcttcatt atctatttct ttaggctcaa acggaatacc
      361 cttacgttca caagcttttt ctaacagata atcagtgatt tcgtaatttt taggtaaaat
      421 tggtccatgt aaataagtac ctaataaatt tttataatga atgccttctt ttttatcttc
      481 atcattatta ccataaccaa aagtaacatg accaagtgta ccgaaatcat gatatgttct
      541 accaccgtga ttttcaaaac ctacaatagt tccaaaagta tcactttcga taacaatatc
      601 tcctgttaat cggtttgtct ttgattcagt ataaaaatct aaaataccta acccttctaa
      661 ttctgtacca tcaggcgtga tatatttttt ccctaaaaat tgatagcctc cacaaatcgt
      721 taatcccggc ataccatctt caatcgcttc tttaagtggt gtcttaattt tacttaattc
      781 ttttgttgct aatgcttgtt ctctatcact tccaccaccg ataaagaaaa tatcacattc
      841 atcaaaggta ataccttctg tttcattgat ttctacgaca ttaactttaa tatttcgttt
      901 tttagcacgt tgtcttaaag caataatatt tcctatatca ctgtataaat tcaatttatc
      961 tgacataaaa tgataaatag tcaattcatg catattatga ttgacctcct tcaaacgaac
     1021 ggtttaattg ttcaagcata ggcgctaatg atgtatagtt tggtattgca actgtgaaac
     1081 ctttataatc catagtcttt gccgttgctt tataaatatc acgctcaact ataattggta
     1141 cttcaacctc tgctaacttc aatcgcaatt gaagttcttc tgctcgtgta cctgtcacga
     1201 tgatagcttc aatttgttgc ttagataatt tttcaaaatc tgcatcataa atccatgaag
     1261 tatctcgacc atctgcagcg ttatcattta gcgaaataac atacactttt tcgccttcta
     1321 attgttcacc aactgataaa cttgcattca ttcctgcagg atttttagct aaattgatca
     1381 tcgcttcttt tcgttctttt ttaaagtact gcatacgacc attgtctgat gtatacgttt
     1441 caaagccatt tttaattgtt tgttcattta accctagctc tcttaaaaca gtatatgctg
     1501 ctaacgcgtt ataagcgtta aagtcacctg caattttcat atcatatttt tcatcattga
     1561 tatttaaata taaaaacggt gccacatcaa aacttgatat ttcatatttt gcttgctctc
     1621 gtttgaaacc acactgacag tgataatgac caatttgatt ataatgaata taatcgtatt
     1681 gcaataagcg accacagttt ggacaatatc tactttcatt catcgtactt tgttcaaatt
     1741 catgggcatg tgctttcata ccatagtaca caatcgtatc acttgcgatt ttcaaacgac
     1801 tcacaaatgg atcatcagca tttagcaata atttgatgcc tttattacta attgtctctg
     1861 caatgttatt aaccataata tcaatttcac cgaagcgatc catttgatct ctaaagaaat
     1921 tagtaaatac catcattgaa ggtgtaactt cttttaacac acgtggaatc gaaccttcat
     1981 caatttcgat taccgcaatt ttagtcttag gtgttgattg catgatgaat gcagaagtta
     2041 tacctgcagc catattagca ccttcattat tgtgtataat ttgaatatta tttgctttta
     2101 aagtatgtcc aattaagttt gaagtcgttg ttttaccatt tgttccactg ataaatacaa
     2161 tatcatcaac ttgctctgct aattttctta atatatctgt atccactttt ctagcgattt
     2221 gtccaggtaa atctgttcct cttttaccta ctgctctact tgctttacgc gccaatttcg
     2281 ctagatggat tgccgtccac tgtctcatgt gtttcctcct caaatttcca ctcgcatcat
     2341 tataacatga caaggcaact tcaaaaaagt ttctcaatca caaattgata cagtgtttct
     2401 aaattataat cattctcatt tagataatta aaaatgatat ttattctcaa ttaaatatcg
     2461 ctaaatgata cattttctca attagagtgc taaaataagg cattaaatta taattattat
     2521 tatttacaat tgagaatgag tttgttatac taagtttaat tcatagtaat tttaatttac
     2581 aaataagagg tgtatcaaaa tgttaagtaa aaatttatta gaagcattaa atgatcaaat
     2641 gaaccatgag tactttgcag cacacgcata tatggcaatg gcagcatact gtgataaaga
     2701 atcgtacgaa ggatttgcaa acttcttcat tcaacaagct aaagaagaac gtttccatgg
     2761 acaaaagatt tataactata ttaacgacag aggtgcacat gcagaattca gagcagtttc
     2821 agcaccaaaa attgactttt caagcatact agaaactttc aaagacagct tatctcaaga
     2881 acaagaagta acaagacgtt tctataactt atctgaaatc gctcgtcaag ataaagatta
     2941 tgcaactatc tcattcttaa actggttctt agatgaacaa gtcgaagaag aatcaatgtt
     3001 tgaaactcac atcaattatt taactcgtat cggcgatgac agcaatgcat tatatcttta
     3061 cgaaaaagaa cttggcgctc gtacattcga cgaagaataa ttaaacatca ctacaataga
     3121 cagataaata tcatacgaca tgataggcat ttgggtcact tacaataacc caatgtctat
     3181 attattttgc tttacggaga tcactagatt cattttctga atcattgatc tgcgtttttt
     3241 cattttcaag gctaattatt gtatttttag tcatttattt tttaaactac taatgttaat
     3301 aactctaaat ttgatgttga attaatttga cgattttaaa gcatatcatc atttactttt
     3361 taatcagagt tacatccaaa tgatagattt cacgttatac cttcacgtat aatattatgt
     3421 atcgtttgta agcaaatgac taaaagtcta ttaatatata catttaatta attgaaagga
     3481 ttgactacat gatacaagat gcgtttgttg cacttgattt tgaaacagca aatggtaaac
     3541 gtacaagtat ttgttctgtc ggaatggtta aagtcattga tagtcaaata acagaaacat
     3601 ttcatactct tgtgaatccg caagactatt tttcacaaca aaatattaaa attcatggca
     3661 tacaaccaga agatgttgaa aatgcaccta cgtttgacta cgtatttcca tatatgatgc
     3721 aatttattgc agatttacct gttgtcgcac ataacgcggc atttgatatg aacgtcttac
     3781 atcaaagcat tcaaaatatt ggtttaccaa ctccaaattt aacttacttt tgtagttatc
     3841 aacttgctaa aagaaccgtt gattcgtatc gatacggttt aaaacatatg atggagtttt
     3901 atcaattaga ttttcatggt catcatgatg cattgaatga tgccaaagca tgcgcaatga
     3961 ttacttttag gctactgaaa aattatgaaa atttaacata tgtaactaat atttatggta
     4021 aaaatctaaa agataaaggc taggactaaa taaaatactc ccttcaaaag taagcattgt
     4081 aaaaatgtaa actttgcagg gagctttatt ttatataaag tcatatatcg tcatattttt
     4141 ataagttgat tgttctaaat tacctacagt gacaccaata agtcgaattg gtacatcagg
     4201 gtcttttaaa tcgttataaa gtaaatatgc aatattataa atatcttctt cagaactaac
     4261 cgaatctctt aaactcatct gtttagatag cgtttcaaat tgataagttt taattttaac
     4321 cgttacagtt ttagctgact tctgtaattt atttagacgt tcagctgttt tacctgacaa
     4381 ttcccatact tttcttaaaa tctcttcatc atcattcacg tctgttgcaa atgtgcgttc
     4441 agtccctact gattttctta ctcttgatga tttcacttca ctatggtcaa taccgcgtgc
     4501 cttgttatat aaaccccgac ctctttttcc aaacaaacgt attaattcaa attccgtttt
     4561 ctcatataaa tctctaccgt taaaaatacc attatcatgc attacttttt tggaagcttt
     4621 acctacgcct ggaaaatctc caatatccaa tgtcatcaaa atatcatgga cattttgata
     4681 atcaatcaca gtcataccat caggtttatt cataccactc gctaatttag ctaaaaattt
     4741 gttataagaa acacctgcag atgctgttaa atgtgtctgc tctagaatat cttttctaat
     4801 atactgagca attttcgaag caggaaggtc tggtctcact aattctgtaa tatctaaata
     4861 cgcttcatcc aatgacatcg gttctacctt atctgtataa cttcggaaaa tagacataat
     4921 ctgcgcagat gtttctcggt aagcaccaaa attacttgtg acaaagtatc catttggaca
     4981 taatttatgc gcttgtgaca taggcattgc tgaatggacg ccgtattttc gtgcttcata
     5041 ggatgccgta gagacaacac ccctactgct tgctttacca ccaacaatga ctggtttccc
     5101 tttcaatttg gggttatctc tcatttcgac ttgtgcaaaa aaatagtcca tatctatatg
     5161 aataattcgt ctctcagtca agtgctcacc tccctactaa tttttacttt tataacgcac
     5221 aaaaatatct caacataatt atacgctgtg tacgattttt ttacataaat cttgcactta
     5281 gcgataacta tattgagata actacaagtt gttataaaat caattgctat ttaagcatga
     5341 tgatgaagac gattgagtaa gaaaacatag gtaatctgaa ataattcaag caaattcatt
     5401 ttgttggtat catcatatta aaatttatta ttgagtcggc ttttgatgat acaaataaat
     5461 actatcttca tcttgttgaa catatttaaa actactagat attgttaatg caacaagcag
     5521 aatatctata aagcaataag cggtatgcat actaaacata aaaataagtg atgaataacc
     5581 aaatacctta attaaaataa gcaagccagt acttaatagg attagtggtg acagcataat
     5641 aattgagaat tgccatttgt tgaagcaagc atctgctgtt tggaataaga ttctgtcttt
     5701 ttttatatta aacataggtt tgctatcttt tttaaataaa agaaataatg ctctatggat
     5761 aagttcatgt aaaatcaata aaataatgaa tccagcaaac ccatatacaa gattgatgat
     5821 gatattttga tcgacaaccg ctgtgacacc taacgcccac ttatacgtaa ataaaatcac
     5881 gaataacgca ataacaagtt gcaagataat aaaccttcgc atttgaaaat tatttgtcgt
     5941 taaatcaatt ttatgcatta ccaaccctcc cgatcatgac attcttattc ttctttaaat
     6001 atagtataca atgtcacatt taatttaaaa agttcatatc aagaaagtaa attggctgta
     6061 ataaaatttt aatatacgac ttctttcttc acttattaag gcgaaatttt atctcaaatc
     6121 atgtgcgcta tttcaaattg aataatgcca ctgtctcaac atgtgttgtt tgtggaaaca
     6181 tatctaccgg tgttacctct tcaagttgat atttttcagc taataataat gcatcacgtt
     6241 gctgtgttgc gggattacat gaaatataga caatacgctt aggttctaat gtaagcaaag
     6301 tctgaataaa cgtttcgtca cagccctttc ttggcggatc aaccattaca acatctggtt
     6361 taatcccttg tgctttccat tgtaaaataa cttcttcagc tttcccacag acaaaagttg
     6421 tattattgca ttggtttata gtcgcatttt gttgtgcgtc ttcaattgca gaaggtacta
     6481 cttcaacacc gtatacatgt tttgcaagtg gtgccatata tagccctatt gttccaatac
     6541 cacaataggt atctaataca acttcattac ctgtcaattg cgcatactca attgctttat
     6601 tatataattt ctctgtttgt tcagaattaa tttggtagaa tgactgatca cttattttaa
     6661 atgtactatc tgttaattga tcaataattg tatctttacc atatagcgtt atagattgac
     6721 gtcccataat aacattagag tggctatcat taatgttttg tttaatgctt gtcacattag
     6781 gaaatgcatc taatatcttc tcaacaacag catttttttg tggccacttt ttaccattag
     6841 ttacaaaaat aatcatcatt tcgtctgtat gatatcctgt tcttacaacc aaatgtctca
     6901 ttaaaccttt tttcaattgt tcttgataaa tacttacatt taaatctttt aaaatagatt
     6961 taacttcatt catcacttct tgatgttgtg aatcttgtat taaacaactt tccatgtcaa
     7021 taatgtcatg gcttctttga cgataaaagc ccataataac ttcattctgt tcattcttac
     7081 caactggaat ctgggacttg tttcgatatc tccaaggatc tgtcatgcca actgtatcgt
     7141 taatcttaga attatcaaaa tgcgcttttc gctgaaacaa attaatcact tgttcctttt
     7201 tcatttcaag ttgtgcttcg tatgataagt gttgaagttg gcacccacca caacgttcat
     7261 aatatataca tggcggtgca acacgattct cacttctcgt atttatatta agcaacttac
     7321 ctatcgcaaa gttctttttt actttgataa ttttatattc aatttgttca ttaattaaag
     7381 cttgtggtat gaaaatagga aagcgatcta tttttacgac accatggcct tcatgcgtta
     7441 aatcaacaac tgttcccgtt tttatgtcat ttttagctat tgcttgcaaa attttacctc
     7501 caaaatgaac aggttaggaa caaaattatg cgcttcctaa cctgccatta tatatttcac
     7561 tatttctgtt tattcttcga ttaaattgtc atcaacatga tcattattta ttaactcttc
     7621 atttacaata tcattaggtg caaagacatc tatatgacgt tctagattta agaaattcgc
     7681 tggtaattta ccaccatatt ctccatctac atttagttgt aagtctgtga atgatgaaat
     7741 attaattgcc tttgcttttt cataaataac tttaggatgc ttagtatgtt ctcctcttga
     7801 agctaaagtc ataatatgac caagttctgc aaggtttgat ttttcaacta taattaacgt
     7861 aaaatagccg tcatctaact tagcgtccgg cactaatttt tcaaatcctg ccattgaatt
     7921 tgttaaacct aaaaagaata ataatgcttc tccttggaaa acattaccat catattcaat
     7981 tcttaaatct acagctttca tttgaggtaa catttcgaaa cctttgatgt aataagcaaa
     8041 tggaccaaca atagatttca atttactcgg tgtttcataa gagacttgcg tcaattgtcc
     8101 gcctgcagct aaattaataa agtatcgatt attcatttta ccaatatcta ctttagtaga
     8161 atgaccttca atgatgacat caagtgcccc catgatgtca ttaggtatat gcaatgcacg
     8221 tccaaagtca ttaacagtac ccataggaat gacacctagc ttaggacgat taggcttttc
     8281 tgcgatacca ttaactactt catttaatgt tccatcacca cctgcagcga ttaatacatc
     8341 ataattttca tgcatagctc tttctgcttc aagtgtggca tcacctattt tctcggttgc
     8401 atatgcactc gtttcatatc ccgctttttc taattttatt aaggcatcag gtaattctct
     8461 tttaaatagc tctttacctg atgtcgggtt ataaatgatt ctagcacgtt tcctcatatc
     8521 ttatccctct acttaaaatt catatatttt aacttcatct ttgtttcgtc taatagggag
     8581 tgggacagaa ataatattta acaaaattta tttcgttcta ccccaacttg cattgtctgt
     8641 agaatttcct ttcgaaattc tctatgttgg ggccccaccc caacttgcac attattgtaa
     8701 gctgacagaa agtcagcttc tttgtttggg ggccccgcca acttgcacat tattgtaagc
     8761 tgacagaaag tcagcttcta tgttggggcc ccactagaat tgaaaaaagc ttgttacaag
     8821 cgtattttct ttcagtcaac tacagccaat ataacattgt agtgcctagg acattgaatt
     8881 tatgacccag gctcagtctt atttcatcat tcttaatatc gttaaagacc aacttgtatc
     8941 ttaaacaaat actatctcaa tatgtacaaa gcttgttatt tattcagcat tttttgccgt
     9001 tcttcattat atagacttcg tcagttatgc tattttacct ttaaaatgat gttgtaaata
     9061 taatgttgtc tttatacaaa aatgttttat acctttttat tatacagttt gccttaatat
     9121 aattaaagct aacgcattaa taaaattaat atttttacca ttaacatgta caatgaataa
     9181 agttaaaagt aatttgactt ctatagatat aaataaaccc tcgattgcat ctaagtcagc
     9241 aatcaagggt ttatttttta aatcttcata gtttgatgat ttaaattatc ttttatctaa
     9301 ttcttgtttt aatagttgat ttactaattg tggattagct tgacctttag acgctttcat
     9361 aatttgacca actaagaagc ccatagcttt gcctttacca tttttgtaat cttcaactga
     9421 ttgttcgtta ttgtctaatg cttcatttac aaattttaga agtgttgctt catcagaaat
     9481 ttgaactaag ccattatctt ccataatctg tttagcatta ccacctttag ctgctaactc
     9541 tgggaagact ttcttcgcaa ttttactgct cattgttccg tcttcgataa gtttaatcat
     9601 acctgctaaa ttttctggtg ttaatttagt atctaataat tctacttgat ttttatttaa
     9661 atattcgttt acgccaccca ttaaccagtt agatgttaat ttaacatctg caccgtgttc
     9721 aattgttgat tcaaagaaat ctgacatttc tttagtcaat gttaatacgt gtgcatcgta
     9781 tgcaggtaaa cctaattcat ttacatactt agctttacgt tcatctggta attcaggaat
     9841 tgtctgacga acacgctctt tccaagcatc atcaatatat aaaggtacaa tgtcaggctc
     9901 tgggaagtaa cggtaatcat cagaaccttc tttaacacgc attaaaattg ttttacctgt
     9961 agattcatca aatcgacgtg tttcttgtcc gatttctcca ccatttaaca attcttcttc
    10021 ttggcgtttt tcttcatatt ctaaaccttt acgtacatag ttaaatgagt ttaagttttt
    10081 caattcggct ttagtaccaa atttttcttg accatatgga cgtaaagaga tgttagcatc
    10141 acaacgtaaa gatccctctt ccatcttaac gtctgataca ccagtgtatt gaataattga
    10201 acgcaatttt tctaaatatg catatgcttc tttaggtgaa cgaatatctg gttcagatac
    10261 gatttcaatt agcggtgtac cttgacggtt caagtcaact aatgaatact cacctttatg
    10321 tgttgactta ccagcatctt cttccatgtg aagacgagta ataccgattc gttttgtttc
    10381 accgtcgact tcgatatcga tatatccatt ttcaccaatt ggttgatcaa attgagaaat
    10441 ttgatatgct tttggattat ctggatagaa atagttctta cggtcaaact tagattctgt
    10501 tgcgatttcc atatttagtg ccattgcagc acgcattgcc cagtctactg cacgcttatt
    10561 aacaactggt aagacacctg gatatgctaa gtcgataaca tttgtatttg agttaggttc
    10621 tgctccaaaa tgcgctggtg atggagaaaa catttttgag tccgttttta actctacgtg
    10681 aacttcaagt cctataactg tttcaaaatg catgatttcc actccttata atttttcata
    10741 aacgtcatgt aaattgtatt gtgtttcata ttgataagcg acacgatata acgttttttc
    10801 atcgaatggt ttaccaatga actgtaaacc gattggtcgg ccatttgatt gtccacaagg
    10861 aacagaaata ccaggtaatc cagctaagtt tactggtgtt gttaataaat cattggcata
    10921 cattgttaat ggatcatcaa tttcttcacc taaattaaac gcagttgtag gcgctgttgg
    10981 accaactact acatcataat tttcgaatac tttatcaaag tcatttttaa tcaatgttct
    11041 aactttttga gattttttat agtaagcatc atagtaacct gaacttaatg caaatgtacc
    11101 taagaaaata cgacgtttta cttctttacc gaaaccttca gatcttgaca ttttatataa
    11161 ttcttctaat gaatgagctt ctttagaatg ataaccataa cgaattccgt caaaacgaga
    11221 aaggtttgac gaagcttctg atgatgcaat cacgtaatat gatggaatac caaatttagt
    11281 atttggcaat gatacttcct caacgacagc acctaaagat tttaaagttt ctacagcgtt
    11341 ttgaactgct tcttttacgt catcagctac accttcacct aagtattctt taggtaatgc
    11401 aacttttaat cctttaatat ctttaccaat ttcagatgta aagtctacat catcaactgg
    11461 tgcacttgta gagtcattaa catctgcacc agaaatagct tctaatacga ttgcattatc
    11521 ttttacattt cgagtcaatg gaccaatttg gtctaatgaa gatgcaaaag caactaatcc
    11581 aaatcgagat acacgaccgt atgttggttt cataccgaca acgccacaat atgcagccgg
    11641 ttgtctaatt gaaccacctg tgtctgaacc taagctaaat ggtactaagc cagctgcaac
    11701 tgctgctgca gatccacctg atgaaccacc tggcactgct ttatggtcaa atgggttaac
    11761 tgtttttttg aaataagatg tttctgttga accacccatt gcaaactcat ccatatttaa
    11821 tttaccgatt aaaacggcat tttcattatg tagtttttcc attacagtag attcgtaaat
    11881 tggcacaaaa ccttctaaca ttttacttgc acatgttgtt tctaatccgt ttgtaataat
    11941 gttatctttt atacccattg gaataccaaa taatttgcca tccatttgat cttttgcttg
    12001 taattcatcc aattcttgcg cttttttgat tgcattttct ttatccagcg ctagaaaaga
    12061 cttaattgtt ggatcagtct cttcaattgc atcatatata tctttaacaa catcagatgg
    12121 tttgattttt ttgtctttta ttaaagttaa taaattctca accgattcgt agcgaatgct
    12181 catcttacgc gtcctcctca ttcatgattg taggcacttt aaattgtcca tcttctgttt
    12241 ctttggcatt tttcaaagct aattcttgtg gaataccttt aattgcttta tcttcacgta
    12301 aaacgttttg taaatctaaa acgtgatatg taggttcaac gccttctgta tcagcgctat
    12361 cattttgttt tgcaaaatct aaaatgcttt ctaatgtgtt ggccatttct tccgtttctt
    12421 caggagaaat ttgaagtctt gcaagattcg cgatatgctc aacttcttca cgtgttactt
    12481 ttgtcattaa taaaagcctc ctttaagtca ttcatcacta aattgtatca aatttccaat
    12541 taaaaatcta agtatttatg aggtgctact ttaatttcat ataaactgta taaacattat
    12601 cattcgttta tcaaatcatt ttttatgaaa acaacactct tttaatatta gacaacccaa
    12661 ttcaatatta tgattatgca attttaatta taaaaatttg ctcatatcca aaaaatacga
    12721 aagcgctttc tatattggta tgcaagtatt tcaaaaagaa taaatttaat tttcctactt
    12781 ttctaaacat ttatctttat gtataatgtt ttcaagtaac taaattataa attaaataaa
    12841 gggagtgttt atcatgctta caatggggac agcattaagt caacaagtag atgccaattg
    12901 gcaaacttat attatgattg ccgtctactt cttgatacta atcgttattg gcttttacgg
    12961 ttacaagcaa gcaactggta acctaagcga gtacatgtta ggtggacgta gtattggacc
    13021 gtatattact gcattatcag ctggagcttc agatatgagt ggatggatga ttatggggct
    13081 acctggttct gtctatagca ctggtctatc agctatgtgg attacaatcg gtttaacatt
    13141 aggtgcttat ataaattact ttgttgttgc tcctagactt cgtgtttata ccgaattagc
    13201 tggagatgca attacattac cagatttctt taaaaatcgt ttaaacgata aaaataatgt
    13261 gttaaagatt atttctggat tgattatcgt agtattcttt acattatata cacattctgg
    13321 tttcgtatct ggtggtaaac tatttgaaag tgcttttgga ttagattatc atttcggttt
    13381 aatattagtt gctttcattg tcattttcta tactttcttt ggtggatatt tagctgtatc
    13441 aattacagat ttcttccaag gtgtcattat gttaattgcg atggttatgg tccctattgt
    13501 tgctatgatg aatttaaacg gctggggaac gtttcatgat gtagcagcta tgaaacctac
    13561 aaatttaaat ttatttaaag ggttatcatt tataggaatt atctctctat tttcatgggg
    13621 attaggttat ttcggtcaac ctcatatcat tataaggttt atgtctatta aatcacacaa
    13681 gatgctacct aaagctagac gtttaggtat tagctggatg gctgttggtt tattaggcgc
    13741 tgtggctgtt ggtttaacag gtattgcatt cgtacctgct tatcatatta aactagaaga
    13801 tcctgagaca ttattcatcg tgatgagtca agtactcttc catcctcttg taggtggttt
    13861 cttacttgct gcgattctag ctgcaattat gagcacgatt tcttcacaat tacttgtaac
    13921 atctagttca ctaacggaag acttttataa attaattcgt ggtgaagaaa aagctaaaac
    13981 gcaccaaaaa gaatttgtta tgattggaag attatctgta ttagttgtag caattgttgc
    14041 catcgcgatt gcatggaatc caaacgacac aattctaaac ttagtaggta acgcttgggc
    14101 cggatttggt gcatcgttca gtccacttgt gctatttgca ctttactgga aaggtttgac
    14161 acgtgccggt gctgtaagtg gaatggtttc aggtgcctta gtcgttatcg tttggattgc
    14221 gtggattaaa ccattggcac atatcaacga aatattcggc ttatatgaaa ttattcctgg
    14281 atttattgta agtgtaatcg ttacatatgt tgtaagtaaa cttactaaaa aacctggtgc
    14341 atttgttgaa actgacttaa acaaagttcg tgacatcgtt agagaaaaat aattcataag
    14401 tcttaacaaa ttaaaaaggt actaatgtta atcaaaatta tgactaacat tggtaccttt
    14461 ttattatctt taatttacaa ttaattactg taaatatgaa cttgcggttc tttgtcatct
    14521 tttgtcttac taattaaagc acgtggttgg ttaccatctt taatacgaat ttcatagtta
    14581 tcgattttat cgaaatattt attcgcttgt tcagtaacgt actgtgtaat accaattgtt
    14641 tcagcttgtc catagtaatc gattggtaaa tctactacta atcgttgtgg ctttttatca
    14701 acaaatttaa ctttccctac tgcttgtgtg aaattagaaa aatatgattg caaattatca
    14761 ttaaattgct tgaaattatt atttaaattt tcatcataat ctgctgctgt tgaagaaggt
    14821 aataaagctg atttttcatt gatattatgc cattcattaa gctttgtttg actcttttct
    14881 gcagtcgctt gagtgataaa ttcacctggt gtgattgaat cttcacttga ttgcttataa
    14941 attgcaaaat gaattggtat atcttttaaa tcatcatttt cacgtaacct tgataatatc
    15001 tcactagcca tttgtttacc ttgcttttta acttcgctat catctagttt tttactaaaa
    15061 gtcgatccat ctttttcttt tttatagtaa taaacactat tcatagctaa accaatcgtc
    15121 atacctttaa tattcttacc ttttgtatct ccaccaccat aaaaatcttg ctctaaaatg
    15181 ttagataaat aggctggtga tttttctgca atcttttcag gatctgtttc accttcgtgt
    15241 gatggattaa gtcctaaatt ttcattcgct ttcttgtctt ttttatcttt ttcagacatt
    15301 ttatcgattt cacgttttgt atacttagga tttaaatagg cattaattgt tttcttgtcc
    15361 aaaaattgac catcttgata caaatattta tctgttggaa atacttcttt acttaagttc
    15421 aataaaccat cttcaaagtc gccgccatta taactatttg ccatgttatc ttgtaaaagt
    15481 cctcttgcct ggctttcttt aaatggtaac aatgtacgat agttatcacc ttgtacattt
    15541 ttatccgttg caatttcttt tacttgattt gaactattgt tatgtttttg attatctttt
    15601 ccagcctggt catccttatg gttaccacaa gcagcgagta taaagatagc tgtaatcaat
    15661 aatactaatg tacgcttcat cgacataccc ctctaactat ttaattcatt ttgcttatct
    15721 acaaattgtt gctctgtcca aatttcaata cctaaacttt gtgcttttgt taattttgaa
    15781 cctgcatctt caccagcaat aacgacatct gtatttttag taacgctact tgtaacttta
    15841 gcaccttgtg atgcaagcca tttagatgct tcattgcgtg tcatttgatg tagcttacca
    15901 gtcagtacta tcgttttacc actaaattca ggatgtcctt caatatctga tgttttgata
    15961 cctttataaa tcatattaac atgtttatct tttaattttt gaattaaagc acgaatatct
    16021 tcattttcta aataagtaac tacagattgt gctactttat cacctatatc atgaatttct
    16081 actaattccg cttcagttac cgttagtaat cgatctatcg tttcatattt ttctgctaac
    16141 acttggctcg ctttaacacc taaatgccta atacctagac caaataataa attttctaaa
    16201 gagttgtcct tagcttgttg aatggcagct aataaattat caactttttt ctgccccatt
    16261 ctgtctaaag gtaataaatc ttcttctgtt aaatagaaaa tatcagcaac atctttaatt
    16321 aattcgcttt gataaagctg ttgaataatt ttagtgccta aaccatcaat attcatggct
    16381 tgtcttgata caaagtgaat caatccttca acaagttgtg cttggcattt tggattaatg
    16441 caacgaagtg ctacttcgcc ttcaatacgt actaattcat gtccacaact tggacaatgg
    16501 gttggcatat gatatgtgac agcatcctca ggtctacgtt ctggaatact acgtacaact
    16561 tcaggtatga tgtcacctgc ttttttcact acaacactat caccaattcg aatatctctg
    16621 tcatgaatta aatcctcatt gtgcaaagat gctcttgata cagttgtacc agctactttt
    16681 actggttcta aaatagcagt aggtgtgact acacctgttc gtccaatact taattcaata
    16741 tctaataatt tagttactac ttcctcagct ggaaatttat aagcaatggc ccatctagga
    16801 gatttttgtg tgaatcccat ctcatcctgt tgatctaaat cattaacctt aataacaatc
    16861 ccatcaatat cataaggtaa tgactctctt tggcttgtcc atttttcaat atactctaaa
    16921 acaccatcga tattatttac acgcgctcta tttttattcg ttgtaaaacc taatttatct
    16981 aactcatcta atgcttcact ttgcgaacgc gcattgaaat cagtgaaatc attgacacta
    17041 tatataaata cgcttagctt tcgttttgcc gttaatttag aatctaactg tcttaatgat
    17101 cccgcagcag cgtttcttgg atttgcaaat aactgctcat catttttttc tttttcttca
    17161 tttaatcgta aaaatgaacg tctcggcata tatgcttcac cacgaacttc tacatttaat
    17221 ggttctttca ttttcaaagg tatcgcatga attgttttta aattttcggt aatatcttca
    17281 cctgttgttc catcaccacg tgttaaacct tgaacgaagt atccatcaac atatttcaat
    17341 gatactgcta agccatcaat ttttaattcg cacatatatt caacgttgcc aatttgttca
    17401 cgtatgcgtt ggtcgaattt tctcaaatca tcctcattaa atgcattccc taaacttaac
    17461 attggcgtgt catggttgac tttattgaaa gaggcttggg cttcaccgcc aactctaact
    17521 gttggagaat ctacagtctt atactcagga tgctcctctt ctattttaat cagttcatga
    17581 agtaatttgt catattcact atctggtaca gatggattat cctctacata gtattcataa
    17641 ctgtattgat ttaataaatc atgtaactcg ttcacacgag acgataaatc agccatccct
    17701 taatcctcct ttttttcaat tggtgcaaat tgcgctaaca aacgttttgg cccttgtgat
    17761 ttaaagataa tatctagttc gattgagcca tttttctcgt ttacattact caccatgcct
    17821 tctccccagg ctttatgcat cactttgtca cctacattcc aatcagatga caatacttgt
    17881 tttttcgttg acgttgttcg ttgactaaat ccgcgtttag caaaaggttt tgccttaggt
    17941 tgtatcgttt gtcgtttgcc acttgaatga ttttctaata gtgattctgg aatttccttt
    18001 aaaaatctgg atggcatatt tgactgaggg cgaccaaata acattcttga tgtcgcatga
    18061 gtgatatata acacctcttc agcccttgta attgctacat aacaaatacg acgttcttct
    18121 tgcatttcat gatcatcttc actcttaatc gctctaatat gtgggaataa agattcttcc
    18181 atccccatta taaagacaat tggaaattca aggcccttag ccgagtgcat cgtcattagt
    18241 gttacgccat tttcagtatc tgcctcatca atatcagcta ctaacgataa atccgttaaa
    18301 aagttaatta atgactgttc ttctaatggg gtattttcct catagtcttt tggtactgac
    18361 ataaattcat cgatgttttc taatctactt cgagattcta atgtattttc acgttcaagc
    18421 atttctcgat agccagactt ttgtaatact tcatcaacta tttcatgaat ttctaaaaat
    18481 tcttgttctt ttatcaagct ttggattaac tcgtaaaaat taagacactc ttgtgtcacc
    18541 ttttttgaca atccgataaa atcagcttct ccaagtgcat caaacatact gatattgttt
    18601 tgaagtgcat agttttgaac tttttcaaca gatgaaggac ctacacctct ttttggaaca
    18661 ttaataatac gttgcaaact aatgtcatca ttactattgg caattatacg caaataactt
    18721 aataaatctt tgatttcttt acggtcatag aacttttggc caccaaccat tgtgtatggc
    18781 atattagatt tcatgaatgt ttcctcaagt acacgtgatt gtgcattcgt tctatataaa
    18841 attgccatat cttgatattt cttaccatta cgttgatgct tcataatttc tcgtattaca
    18901 aattccgctt catcacgttc cgtcattgct tcatagtaat gaattttctc accattcgta
    18961 tttgcagtcc acagtccttt tggcttacgt tcagaattat ttttaatcac ttcgttagcc
    19021 gcatttaaaa tcgttttcgt cgaacgataa ttttgctcta aaaagattgt attcgcttct
    19081 ggatagtctt tttcaaatga taagatattt tgaatatcag caccacgcca accataaatt
    19141 gactgatctg agtcacctac aacacataag tttttaaact tacttgctaa taatttaact
    19201 aatgtgtatt gtgctttatt agtatcttga tactcatcta catgaatata ttggaatttg
    19261 ttctgataat attctagaac ttctggtaca cgctcaaata agttaatcgt tgtcataata
    19321 aggtcatcaa aatctaacgc ttcattacgt gacaattggc gttgatatcc actataaacc
    19381 gttgctacca tttgcgagtg ataatctgtg gcttcttttt gagcatctgc aggtgtttta
    19441 agttcatttt tcaaattact gatcgcaccg ataaacatac gaggttcaaa ctttttacta
    19501 tcaatatttt cattttttaa gacgtcttta ataacagatt tttggtctgt aggatcaatt
    19561 atcgtaaaat tgcgttctat accaattcga tctgcatcac gacgtaaaat acgaacacac
    19621 attgagtgga atgttgacat ccaaataact tctgcttgat cacctactaa tttttgaaca
    19681 cgttctttca tttctcttgc agctttattt gtaaaagtaa tagccaaaac attgtatggt
    19741 gagacatctt tttcgtctaa taaataagca attctatgtg ttaaaacacg tgtcttccct
    19801 gaaccagcac ctgccataat taacaatggt ccttctgttg tctttacagc ttcactttgc
    19861 tctgtattca tatgatttaa taacgcattc atttactaga ctcctttatt tttactgttt
    19921 ttaaagcttt tttaatatct ttataaataa tatcaccgac gataattgta tctgcaatag
    19981 ctgccatctc tgtcgcttgt tgttctgagg aaataccgcc accataaaaa agttgcgttt
    20041 ctgttagatg ttctgagaca gcttgaacct ttgatacgtc gccataaatg ccactatact
    20101 ctatatacat aaccggtaat cgatacatat gattgaccat ttgggcatat gcttctaaat
    20161 cttctgttgt taaatctgta tttgctttgg tatgttttgc cattttgcta tcagcattgc
    20221 acacgacata cccttcaaat attacttcct caaaatctat actatgtcca tatgttttaa
    20281 gcgcttctaa taatgtacca ttgtgaaata caacatctgt actgttcagt actgtaggta
    20341 caaaataaaa atcaaaacca ggcattacac tttcgatgtt tgatatttca agtactaaag
    20401 gcaatgggta tcttcttact ctgctcatta aatgaatgac attatcttca gtaacgtcat
    20461 cagttccacc aatcataatt gcatctgttt gagacataca aatcgcatct aaatcatcat
    20521 ctgaaatatg tttagctggg tctaatttaa aaatatggcg ccatttttta atgtcataca
    20581 ttttccgaaa ctcctttttt aacatacata tgattatagc atttttaagt gtcgagttct
    20641 aaagacttaa tatgaaattt aagttctttt actctgtaaa ttttttcgtg aaaaaacaat
    20701 gattatctac cttattagtg cagatagata accattgttt atcgttcatt tattattcat
    20761 ttgtttcaat attcatacga tctaaaatca ttgtataagc atcattaccc cattgtaatg
    20821 aacgcttcac tctagaaatc gttgcagtcg atgctccaga ttcttgttca atcgttgcat
    20881 aggtataacc ttgcttaatc attttagcaa cttgtaatct ttgagacagt gattgaattt
    20941 catttacagt acacaaatca tcgaaaaatt ggtaacattc ttctctattt tctaacgtta
    21001 gtattgcatc aaacaattca tctaacgctg cacctcgtaa tttttcaatt tgcatatcga
    21061 aacaacccct ataattttat tctttatgtc attttaacgc attaatctat taaagtgtag
    21121 cgatttatat tttattaaat ctgaatcgtt actttattta attttatgct aatccagcgc
    21181 gttcgaaaat agtgtcaact tgattcaaat gatgtttagg atcgaaacat tcatccaatt
    21241 cttcttttgt taaaacactt gtaatagact catcttgttc gattaattca cggaacggtg
    21301 ttttcgtttc ccaagatatc atcgcttttg gttgtacttt gtcgtatgct tcttcacgaa
    21361 ccataccttt attaattaat gctaataaga cacgttgtga gaaaatcaga ccaaatgttt
    21421 tatctatgtt attacgcata ttatcttcaa atacagttaa acggtccaca atatttgtga
    21481 aacgattcaa tgcataatct agtgctattg taacatctgg taacataata cgctcagcag
    21541 aagaatgaga aatatctctt tcatgccata atggcacatt ctcataagct gtagtaatat
    21601 aaccacgaat gactcttgaa atacctgtga tattttcaga accaattgga tttcgtttat
    21661 gaggcattgc agatgaacct ttttggcctt ttgcaaatgc ttcttcaact tctctcgttt
    21721 cggttttttg aaggttacgt atttcaacgg caaatttttc tagtgatgtc gcgattaatg
    21781 ctaatgtcgc aatatagtat gcatgtcgat cgcgttgcaa tgtttgcgtt gatacaggcg
    21841 ctgtgccaat acctaaatgt ttacacacat aactttctat ttcaggagga atgttagcaa
    21901 aagtacctac tgcaccactc attttcccta cttcaatttc ttctcttact tgtttgaaac
    21961 gttgtaagtt acgttgcatt tccgtgtacc acaatgccat tttgacacca aatgtagttg
    22021 gttctgcatg cactccatgt gtacgtccca tcatcaatgt atatttataa ttttttgctt
    22081 tttcagctaa aacgtcgata aatctttcta aatctttttc aataatgtca tttgcttgtt
    22141 taataacgaa acttaaagct gtatctacaa catcagtaga agttaaacca taatgtaccc
    22201 acttacgttc ttcacctagc gtttcagaaa cttgtctagt aaaggctaca acatcatggc
    22261 gcgtttcttg ttcaatttct tgtgcacgtt cgacatttac ctttgcgttt tgacgaattt
    22321 tttgtacgtc agctttcggt atatgtccta attcactcca tgcttcacat gctaaaattt
    22381 ccacttctaa ccatgcttca tagcgatttt gatcagtcca aatattagac atttcttctc
    22441 tagaatagcg ttcaatcatt tttttaactc ctatcgtatg tatactcgaa ttttcaaaaa
    22501 cagaacattt tttaataaaa ataaacaaaa agttcgtttt taactctact cttcacagtt
    22561 taacagaatt ggttataaat gtataaaaaa tttccgtata ttctcattct attttatttt
    22621 ttacattaat acaattatct atggcagatt aatactgatt tctaattgta aatgttcgca
    22681 atactttaat ctaattggta tttataatga tccattgaga atatattttt gcattttagt
    22741 ttttctcccc tcaactacac tatccaactt tttattacaa ttaggacaat caatagcttt
    22801 agtaatacca gaatttattc actttacttc tcctattgta tggaattttc aacaatctac
    22861 ttttcctagt ttcataatat ttcttcttac atttcataat atgttttaaa attattcgct
    22921 tctaatataa caaattttct actcacaaaa agaatatata ttctattgca taggtgtggc
    22981 attttaaata taagacatta atattgaact ccagaatcgt ataaatacga aactga
//
LOCUS       DANJFZ010000032        22502 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.032, whole genome shotgun sequence.
ACCESSION   DANJFZ010000032 DANJFZ010000000
VERSION     DANJFZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.032"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..495
                     /locus_tag="PWN45_001109"
     CDS             <1..495
                     /locus_tag="PWN45_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0622888.1"
                     /translation="NPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFIALH
                     IASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRRLRR
                     KEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHFEER
                     INQS"
     gene            470..637
                     /locus_tag="PWN45_001110"
     CDS             470..637
                     /locus_tag="PWN45_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622889.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            630..1838
                     /locus_tag="PWN45_001111"
     CDS             630..1838
                     /locus_tag="PWN45_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0622890.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            2319..4841
                     /gene="mprF"
                     /locus_tag="PWN45_001112"
     CDS             2319..4841
                     /gene="mprF"
                     /locus_tag="PWN45_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0622891.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(5044..5553)
                     /gene="msrA"
                     /locus_tag="PWN45_001113"
     CDS             complement(5044..5553)
                     /gene="msrA"
                     /locus_tag="PWN45_001113"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0622892.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            5690..6673
                     /locus_tag="PWN45_001114"
     CDS             5690..6673
                     /locus_tag="PWN45_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0622893.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(6761..6946)
                     /locus_tag="PWN45_001115"
     CDS             complement(6761..6946)
                     /locus_tag="PWN45_001115"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL0622894.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            7094..8356
                     /locus_tag="PWN45_001116"
     CDS             7094..8356
                     /locus_tag="PWN45_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0622895.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(8493..9584)
                     /locus_tag="PWN45_001117"
     CDS             complement(8493..9584)
                     /locus_tag="PWN45_001117"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL0622896.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            9749..10780
                     /locus_tag="PWN45_001118"
     CDS             9749..10780
                     /locus_tag="PWN45_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0622897.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            11272..12678
                     /locus_tag="PWN45_001119"
     CDS             11272..12678
                     /locus_tag="PWN45_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL0622898.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            12675..13241
                     /locus_tag="PWN45_001120"
     CDS             12675..13241
                     /locus_tag="PWN45_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL0622899.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            13247..14245
                     /gene="trpD"
                     /locus_tag="PWN45_001121"
     CDS             13247..14245
                     /gene="trpD"
                     /locus_tag="PWN45_001121"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL0622900.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            14247..15029
                     /gene="trpC"
                     /locus_tag="PWN45_001122"
     CDS             14247..15029
                     /gene="trpC"
                     /locus_tag="PWN45_001122"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDL0622901.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            15029..15661
                     /locus_tag="PWN45_001123"
     CDS             15029..15661
                     /locus_tag="PWN45_001123"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDL0622902.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            15654..16868
                     /gene="trpB"
                     /locus_tag="PWN45_001124"
     CDS             15654..16868
                     /gene="trpB"
                     /locus_tag="PWN45_001124"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL0622903.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            16861..17589
                     /locus_tag="PWN45_001125"
     CDS             16861..17589
                     /locus_tag="PWN45_001125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL0622904.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            17911..19173
                     /gene="femA"
                     /locus_tag="PWN45_001126"
     CDS             17911..19173
                     /gene="femA"
                     /locus_tag="PWN45_001126"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDL0622905.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            19192..20451
                     /gene="femB"
                     /locus_tag="PWN45_001127"
     CDS             19192..20451
                     /gene="femB"
                     /locus_tag="PWN45_001127"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDL0622906.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            21140..21907
                     /locus_tag="PWN45_001128"
     CDS             21140..21907
                     /locus_tag="PWN45_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0622907.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(21955..22428)
                     /locus_tag="PWN45_001129"
     CDS             complement(21955..22428)
                     /locus_tag="PWN45_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDL0622908.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT         7919 a         3210 c         3982 g         7391 t
ORIGIN      
        1 aatccatttg ttatggaaac tatgcagtta tatagtgatg catatcatat tgctaaacag
       61 gtgattgatc agttaaatgc agcattagat gtacattttc ctgaagatga gataggattt
      121 attgcattac atattgcatc taatacagaa gatttatcta tgcatgagat gaccttgatc
      181 aataatgtta ttaaaaaagg tatagatatc attgaatcag accttgtgac aactgttgat
      241 aaggaatcat tacaatacca acgttttata aggcacgtac aatttttaat tcgccgatta
      301 agaagaaaag aatatataca tgcacaagat gattttgtgt ctatgattaa aaatcactat
      361 ccgatttgct ataacacagc atataaaatt ttaactatga tacaaaaaca atttgatgtt
      421 aatatcagtg agtctgaaat tatatattta acattacaca ttcatcattt tgaagaaagg
      481 attaatcaat cctaaattaa gtgtattaca cgggcgtttc gttactgtaa agggtatttg
      541 tatggtatca ttgatgttag taattttaca taagtggact gtttttatac aacatcttat
      601 gaatatttta agtggtggtg aatctataat tgaatgaaaa tgaaaagaat ataagaaaga
      661 attttttaaa atttaccgaa tcacggtata tgaagtttgt tggtgggaat gatttagtct
      721 tctcattaat agcgctagta ttgttgggta ttgttatttt tattttcgaa aaagtatcat
      781 atgtttttga tccttttatc atcgttttta agacgatagc agcacctatc atcgtctctt
      841 taattctatt ctatctattt aacccaatcg taaatatgat ggaacgttat agaataccaa
      901 gagttgcagg tatttctatt atttatctag ctgtagtagg tgttattacg ttaattgtta
      961 atttattgat acctattatt ggttcgcaag tagatagttt agttaaaaat tcaccgcaat
     1021 atctagaaaa attaattaat tctattgata aaatagcaaa taatacgttt ttctcttcgt
     1081 attatagtca aattaatgat tggttaaatt ctttacctaa gaaaatacca tctatgttaa
     1141 gtgaatttac agatggcttt gggtctaaaa ttgcaacgtt tgcagaaacg attgctaata
     1201 ttggcgttgt gattgtcaca acaccatttg tactattctt tatgcttaaa gatggacatc
     1261 acttcaaaga attttcaacg aatattatgc caccgaaatt ccgaaaagat tttcatgatc
     1321 tacttgaaaa aatgagtgtt caagttggtt catacattca aggacaaatt atcgtttcat
     1381 tctgtatcgg tatactgttg tttatcggtt attcggttat cgggttgaaa tatagcttag
     1441 tattagctag tattgcggca gttacaagtg ttgtaccata tttagggcct actatagcga
     1501 tttctccagc tattgtaata gctgctataa catcgccgtg gatgctctta aaattagcag
     1561 tagtatggac tttagtacaa tttgttgaag ggcacttcat ttcaccaaat atcatgggta
     1621 aaacacttaa gattcatcca cttacaatca ttttcatttt actgtgtgca ggcaaattgc
     1681 ttggtattgt aggcgttatt ttaggtattc cgggatatgc tattttaaaa gtattagtta
     1741 ctcatttatt ccaattattt aaacgtcgat acaatcgttt ctatggtaat gatgtaggtg
     1801 aatatgatat taaagaaagt aataaaatag ttgaataagt aataaaaaat accaatgact
     1861 atatgtttgt tggtattttt tatggtgctt ataatgttgg gcagttacat ttatgattat
     1921 cagcacatgt ttagcttata aatttttacg aaagtttagc cgtttataaa gcacatgcat
     1981 aatgaaacga gtatttgcca cttgattagt acttcattat tatgtcgaaa ataaaaataa
     2041 gtggtatttt taatatatta agaagcactc ataatcggct gttaattaat aatatttttc
     2101 ataagtattg attcatcatt ttctttatgt taaatatgaa tcagtataga taaccatatt
     2161 gttctgtttg agatagaaat attttctatt tacttcttaa tacagatatc aatatgacaa
     2221 aagtgttata taaatcaaag gtaaatgata tgctattttt atggtttata tgatatattt
     2281 tgatttataa cagaaataat tagaattgat gtgaaaaaat gaatcaggaa gttaaaaaca
     2341 aaatattttc aatcttaaaa attacgtttg ctacagcttt atttattttt gtagcaatca
     2401 cattgtatcg ggagttatct ggtattaact ttaaagatac gttggttgaa tttagtaaga
     2461 ttaaccgtat gtccttagtg ttactattta ttggtggtgg ggcatcgctt gttattctat
     2521 caatgtatga tgtgatttta tctagagctt taaaaatgga tatatcctta ggcaaagttt
     2581 taagagtaag ttatatcatc aatgcattga atgcgattgt aggtttcggt ggctttattg
     2641 gtgcaggcgt tagagcaatg gtttataaaa actatacgca tgataaaaag aaattagttc
     2701 actttatatc cttaatactt atttcaatgt tgacaggttt aagcttatta tcattgctaa
     2761 ttgtattcca tgttttcgat gcatctttaa tcttagataa gattacatgg gtaagatggg
     2821 tattatatgt agtgtcattt ttcttaccat tattcattat ttattcaatg gttagaccac
     2881 ccgataaaaa caatcgtttt gtaggattgt actgcacttt agtgtcgtgt gttgaatggt
     2941 tagcagctgc agttgtatta tatttctgtg gtgtaattgt tgacgctcat gtatcattca
     3001 tgtcctttat tgcaatattt atcattgctg cattatcagg tttagtcagc tttattcctg
     3061 gtggtttcgg cgctttcgat ttagttgtat tactaggatt taaaacttta ggtgtccctg
     3121 aggaaaaagt attattaatg ctacttctat atcgttttgc gtactatttt gtaccggtaa
     3181 ttattgcatt aattttatca tcatttgaat ttggtacatc agctaagaag tacattgagg
     3241 gatctaaata ctttattcct gctaaagatg ttacgtcatt tttaatgtct tatcaaaagg
     3301 atattattgc taaaattcca tcattatcat tagcaatttt agtattcttt acaagtatga
     3361 tcttttttgt aaataactta acgattgttt acgatgcttt atatgatgga aatcacttaa
     3421 cgtattatat tctattggca attcatacta gtgcttgttt attactttta ctgaatgtag
     3481 ttggtattta taagcaaagt agacgtgcca ttatctttgc tatgatttca attttattaa
     3541 tcacagtggc gacattcttc acttacgctt catatatttt aataacatgg ttagctatta
     3601 tttttgttct gcttattgta gctttccgta gagcacgtag gttgaaacgc ccagtaagaa
     3661 tgagaaatat agttgcaatg cttttattca gtttatttat tttatatgtt aaccatatat
     3721 ttattgctgg aacgttatat gcattagata tttatacgat tgaaatgcat acatctgtat
     3781 tgcgctatta cttctggctt acgattttaa tcatcgctat catcataggt atgattgcat
     3841 ggttgtttga ttatcaattt agcaaagtac gtatttcttc taaaattgaa gattgcgagg
     3901 agattattaa tcagtacggc ggtaattatt tgagtcactt gatatatagt ggtgacaagc
     3961 agtttttcac taatgaaaat aaaacagcat ttttaatgta tcgttataaa gcaagttcat
     4021 tagtggttct tggagatccg ttaggtgatg aaaatgcctt tgatgaattg ttagaagcat
     4081 tctataatta cgctgagtat ttaggctatg atgttatatt ctatcaagtt acagatcaac
     4141 acatgccttt atatcataat ttcggtaacc aatttttcaa attaggtgaa gaagcaatta
     4201 ttgatttaac gcaattttca acttcaggta aaaaacgccg tggatttaga gcgactttaa
     4261 ataaattcga tgaacttaat atttcgttcg aaattattga accaccgttt tcaactgaat
     4321 ttataaatga acttcaacat gtaagtgatt tatggctaga taatcgtcag gaaatgcatt
     4381 tctctgttgg tgaatttaat gaagaatact tatctaaagc gccaattggt gtaatgcgaa
     4441 atgaagaaaa tgaagtaatt gcattttgta gtttaatgcc aacatacttt aatgatgcca
     4501 tttcagtcga tttaattaga tggttgccag agttagattt accattaatg gatggtctat
     4561 acttgcatat gttactttgg agtaaagaac aaggttatac aaaatttaat atgggtatgg
     4621 caacgttatc gaacgttggt caattgcatt attcatattt aagagaacga cttgcaggcc
     4681 gtgtctttga acatttcaac ggtctatatc gtttccaagg attacgtcgt tataaatcta
     4741 aatataatcc gaattgggaa ccacgctttt tagtttatcg taaagataat tcgctttggg
     4801 aatcactttc taaagtaatg cgtgtaatac gtcacaaata attaaaatcc aagtgctaag
     4861 aggtatacag ttatgcctga tgcgcttgga ttttttggtt tctataaaga atgatttata
     4921 aaaaacggta cagataagaa tatttaagtt tcctatctgt accgtatttt aatattgaaa
     4981 tgagctaaag ttaagtcgcc taacttttat tatcagtcca atcagtttca tcaagtataa
     5041 atgttattgc ttatttttgt attcttggcg tattttttgt tcttctgcat agcgctctgg
     5101 atttttctta taaaaatctt ggtgatagtc ttcggctttg taaaattgtg acgctggtaa
     5161 tatttttgtt gcaattgcct tatcagcatt aatcgtattt ttaagctgct cgatataagt
     5221 ctcagcgagt tctttttgat gatcattagt gtagaaaata gctgtttgat attgaggacc
     5281 acggtcttga tattgaccac ctgtatctaa tgggtcaatg actgagaaaa atatttctaa
     5341 taacttattg tatgagaata atgcaacatc atattgaatt tcaacagttt ctaaatgacc
     5401 actcgtacct gattttactt gttcgtaagt aggattttca atatgtccgc ccatatatcc
     5461 agaagttact ttttctatgc cgtcaaaggt gtcaaatggt ttcgtcatac accaaaagca
     5521 acctccggca aaataagctg tattaatatt catttttgac atcctttcat tagaccttag
     5581 tacgatttat taagaaatca cttgcttttt gaattgtttt tatataacgt taatatgtga
     5641 ttattatagt acacaaaaat ctatttttga agatataagg taggtaaata tggataaaga
     5701 aactaatgac aacgaatata gacgtcaaag tgaacatcgc acttcggcgc ctaagcgaaa
     5761 aaagaagaag aaaattagga aattacctat cattcttctg attgttgtaa ttttacttat
     5821 cgcattagtt gtatatattg tacatagtta caatagcggt gtagaatatg ccaagaaaca
     5881 tgcgaaagat gttaaagtac atcaatttaa tggaccagta aaaaatgatg gtaaaatttc
     5941 tattcttgta ctcggtgcag ataaagcaca aggtggacaa tcaagaacag attctatcat
     6001 ggttgttcaa tatgacttta tcaataaaaa gatgaaaatg atgtctgtca tgcgtgatat
     6061 ttatgcagat attccaggat atggaaaaca caaaattaat tcagcatacg ctttaggtgg
     6121 tccagagcta cttagaaaaa cacttgataa aaatttagga attaatcctg aatattatgc
     6181 agtagttgat tttactggat ttgagaaaat gattgatgaa ttaatgcctg aaggtgtacc
     6241 aattaatgtc gaaaaagata tgtcgaaaaa tattggtgta tctttgaaaa agggtaacca
     6301 taggttgaat ggtaaagaat tacttggtta tgcaagattc cgtcacgacc ctgaaggtga
     6361 cttcggacgc gtgcgacgtc agcaacaagt gatgcaaaca ttgaaaaaag aaatggttaa
     6421 ttttagaaca gttgttaaat taccaaaagt tgcaggtatt ttaagaggct atgtgaatac
     6481 aaacattcct gattcaggga ttttccaaac aggtttgagt tttggtatcc gaggtgaaaa
     6541 agatgttaag tcattgactg tgccaatcaa gaactcatac gaagatgtca atacaaatac
     6601 tgatggtagt gcattacaga ttaataaaaa cacaaataaa caagctatta aagacttttt
     6661 agatgaagat taaaaataaa caaggcgatt tctatcatac tcagatagaa gtcgccttgt
     6721 tcgttttcaa agaattaatt ggcatgcatc ttatgaagaa ttattgatct gactttctta
     6781 cgccagccac accataatgg tttggtttca tttcttctat aacaacgtga attgcttgtc
     6841 tatttgcccc cgttgttttt tctacggcgt cagttacttc gctaactaaa ttttttaatt
     6901 gttcatccga acgaccttct aataatttta cattgacgat tggcatcatc caaacctcct
     6961 aatcattatt aatttgagta tatcatgtaa tacttaaaaa ttcatttgca aaataacaga
     7021 acatttgttc taaaatagtt gaaatagaac acgtgttcgt atataataag attcaagtaa
     7081 gggaggaata acagtgtata attatcattt attagaagat agggatgttc tatgtattga
     7141 ccaaaaaagt ttttttgcga gtgtttcttg tattgaaaag gggctagatc cattagaaac
     7201 aaagctagct gttgttgcag atactaagcg tcagggttct gtaatattgg ctgcgacacc
     7261 taaattaaaa gaattaggca tcaagacagg gtcgcgattg tttgaaatac cacatagaaa
     7321 tgatatttac attatcaatc caagtatgcg taaatatctt aatgtttcag ttgctatttc
     7381 taagattgca ttgcgttata ttccacctga agatttacac caatatagta ttgacgaatt
     7441 ttttatggat gttactgata gctatcatag atttagttct acagtacatg cattttgcga
     7501 aagacttaaa cgtgaaattt atgaagaaac aggcatttat tgtactgtgg gcattggttc
     7561 taatatgtta ttaagtaaaa ttgctatgga tgttgaagcg aagcatagtc aaaatggtat
     7621 agctgaatgg cgatatcaag atgtaccaac gaaattatgg ccaattcagc ccttgcgaga
     7681 tttttggggt attaatcgtc gaacagaagc caaattgaat aaaagaggaa tttttactat
     7741 aggagattta gcgaaatatc catataaatt tttaaaaaaa gagttcggta ttttaggtgt
     7801 tgatatgcat ctacatgcga atgggataga tcagagtaaa gtacgtgaaa agcacaagat
     7861 cagcaatcca tcgatatgca aaagtcaaat attaatgaga gattatcatt ttgatgaagc
     7921 aaaagtagta atgcaagagt taattgaaga tgttgctagc agagttcgag caagaaaaaa
     7981 agtggcaaga acgatacatt ttgcctttgg ctatagtgat gaaggcggtg tacataagca
     8041 atatactttg aaagatccaa caaacttaga aaaagatatt tataaagtag taatgcattt
     8101 cgcagataaa ttatgtaata aacaagcact atatcgtacg ctaagtatat ctttgagtca
     8161 atttattaat gaggatgagc gacagttaag tctgtttgaa gatgaatacc aacgcaaacg
     8221 tgacgaatgt ctagctaaaa cgatagacca attacatttg aaatacggca aaggtattgt
     8281 gtccaaagca gtatcgttta cagaagcagg tacaaaacac ggcagattag gtttaatggc
     8341 tggacataaa atgtaatgac tatacggttt aagtaatata taactgtgat tcgtataaaa
     8401 taagtctcta aagataaata tttcatatat cacaatagat tttcacaata atatctaaga
     8461 atacatggaa tttatcaaaa gagacttaat aattattgga tataacaatc aaaatcactc
     8521 aatgcttgca taccgcgttc tcggtcagta gggtttttga aactaatttt taaagcaccg
     8581 tatatatctt cgcgtacttc taagattctt aagttgctta tagatatgtt atgtaaactc
     8641 aggatataag tcactttact tatcatacct gattcatccg gaatgtctac atatagatca
     8701 tacgcagtat ttagtccacc tagttgttta gcgggtagtg cgtcgcgata cgatttagct
     8761 tgggcaaaaa atgataacaa tttttcagaa tcattgcttt caattagtct ttctaaatct
     8821 tgaaactgac tttttagctg tcgaatcatt tctaaaatat acgttttatt actcaaggtg
     8881 atatctttcc acatttgtgc attactacta gctatacgag tgatatcacg aaaaccacca
     8941 gctgcaagtt tattaactaa atgatgttct tgaccgttct tttgactaac atgaactaaa
     9001 ctagatgcaa cgatatgagg taaatgactt acgacgcttg ttacgtagtc gtgttcttca
     9061 gcagtagtta caataaattt agcaagagta ggtgataaca gttcttttaa cgtgtttgct
     9121 gcttgctcat ttcttggctc attgtagact aaaatataat aagcgttttc aaataagtgc
     9181 tttttagcat ttagtacacc agatttatga ctaccagcca ttggatgacc actgactaaa
     9241 tgaatattat gctttaataa attgcattcg tgttgctgta tcattgcttt agtactacca
     9301 gtatcagaaa caataacacc aggtttagtt ggcatatcta taagctcgct aagatattta
     9361 tttgtgatag caacaggtgt tgcataaata attacatcgg cttttttaat agcttcacta
     9421 taatttaaac atttttcatt aataatgccg attgatttag ctttatctaa ctgagaagta
     9481 tctgcatcgt atgcaataat attagtatta gggttatggt attttatatt gctagcaaga
     9541 cttccaccaa ttaatccaag cccaacaaat aaaactgttg tcatataaat caccttattt
     9601 cgaaattttc agaataataa cattgtaaat gagctgttga cacagtgcaa tagtaaataa
     9661 aaatcgataa tagcattaat agataaacgg agataaatca tctacaataa agagtatagt
     9721 aacacaatgg caacggaggg gtaaatcaat ggaaccaata ttagaaatga ttaaaacatt
     9781 aacaggtatt aatagtcctt caggagacac agaagaagca attcaatttg tcgaaaaata
     9841 tgcaaaagac ttgggttatc aaacaacact aacaaataaa ggtgcgttat taataacagt
     9901 gccaggcaaa aatgatgaag tacaacgctg tattactgct catgttgata ctttaggtgc
     9961 aatggttaaa gaaattaaag aagatggtcg cttagcaata gaattaattg gaggattcac
    10021 gtataacgcg attgagggtg aatattgcca aattaaaact gatgctggtc aaatatatac
    10081 aggaacaatt tgtctgcatg aaacaagtgt tcatgtatat agaaataatc atgaaatacc
    10141 tagagatcaa aagcatatgg aaataagaat tgatgaagta actacatcag aagaagatac
    10201 aaagagttta ggtatttcag taggtaattt tgttagcttt gatccacgta cagttatcac
    10261 gtcatcaggt tttattaaat ctcgtcattt agatgataaa gctagcgtag cgatgatact
    10321 acaattacta aagaaattaa aagaagagca aataatatta ccacatacaa cgcaatttta
    10381 tatttctaat aacgaagaaa taggttacgg tgcaaatgca tcaattgatt cgaaaatcaa
    10441 agaatatatt gcattagata tgggcgcgtt gggagacggt caagcatcgg atgaatatac
    10501 agtttctatt tgtgccaaag atgcttcagg tccatatcat aagcaattga aatcgcacct
    10561 agttaatctt tgcaaaataa ataacattcc atataaagta gacatatatc catattatgg
    10621 ttcagatgct tcagcagctt tacatgctgg tgcggatatc agacatggtt tatttggcgc
    10681 tggcattgaa tcatctcatg caatggaacg aacacatatt gattctatta aagcgacaga
    10741 gaaattacta tatgcatatt gcttatcacc aattgagtaa acaattagtg ttgacaaatg
    10801 tgaacgacct atgtaatata atgaactata aaaataatta gaattttcta aagaaatagt
    10861 agcagatatg aaacgtagca aatagaaagc taatgggtga tgggaattag cacgccatat
    10921 cttgtgaatt ggactttgga aaacaattga atgagttttg aaagtgaaca tgaattatgt
    10981 taactaaggt ggcaccacgg taacgcgtcc ttacaggtat atgcgttatg tggtgtcttt
    11041 ttatttagac aaaatgtagt agttaattaa aggtagcaac agaaagttag tggatgatgt
    11101 gaactaacac cgagattaat gaaattgggt tttgtctgca acagaaaaat tatatatagt
    11161 aaagagtgaa ctatgaatat ttcgaatatt cggttaattt aggtggtacc acgcgtcagc
    11221 gtcctttata ttgataagga tgctggcgct tttttgaaag gagcgtatag aatggatata
    11281 ttttataaaa aaataaaagc aaatgtaacg cccgaagttt tagcacaact tcattccaag
    11341 aagatcattt tggaaagtac aaatcaacaa caaactaaag gtcgctattc agttgttatt
    11401 tttgatattt atggcacttt aactttagat aatgatgtat tatcagtaag tactttaaaa
    11461 gaatcgtatc aaatcactga aagaccgtac cattatttaa cgactaaaat aaatgaagac
    11521 taccataata ttcaagatga gcaacttaag tcattaccat ttatatctgg atatgttggg
    11581 acgtgtagct ttgatttagt aagacatgaa tttcctaaat tgcaatcaat acaattagaa
    11641 gatcacaagc agcacgatgt aaggttatat atggttgaac aagtttatgt atttgaccat
    11701 tacaaagatg agttatatat catcgcgacg aatcaatttt caaattcaac aaaatcagat
    11761 cttgagaatc gagttattaa gtctatcgaa gacttaacta aaatccaacc attcatgcct
    11821 acacaagatt ttgattttaa aactaaagaa attcaatcaa acatttctga agaaagattt
    11881 atcgaaatga ttcagtattt caaagagaaa ataacagaag gggatatgtt ccaagttgtg
    11941 ccatcaagaa tttacaaata tgcgcatcat gctagtcagc atttaaatca actttcgttt
    12001 caactgtatc aaaatttaaa acgacaaaac ccaagtccat atatgtatta tcttaatatc
    12061 gatcaaccat atattgtcgg tagttctccc gaaagtttcg taagtgtcaa agatcaaatt
    12121 gtaacaacta atcctattgc aggtacgatt caacgtggtg agacgacaca aatagataat
    12181 gagaatatga aacaactact taatgatcca aaagaatgca gcgaacatcg tatgctagtt
    12241 gatttaggac gtaatgatat tcatagagta agtaaaatcg gtacctcaaa aattactgaa
    12301 ttaatggtta ttgaaaaata tgaacatgtt atgcatatcg taagtgaagt cacaggtaaa
    12361 ataaatcaaa atttatcgcc aatgacagtt attgcgaatt tattaccaac aggtaccgtt
    12421 tcaggtgcac caaaattacg tgcaattgaa agaatatatg aacaatatcc acataaacgg
    12481 ggcgtttata gtggtggtgt tggatacata aattgtaatc ataacttaga ttttgcatta
    12541 gcaattcgaa cgatgatgat agatgagcag tatatcaacg tagaagctgg ttgtggcgtt
    12601 gtatatgatt ctattcctga aaaagaactg aatgaaacga aattgaaagc taaaagctta
    12661 ttggaggtga gcccatgatc ttagttgtag ataattatga ttcctttaca tataacctag
    12721 tggatattgt tgctcaacat actgacgtca ttgttcaata ccctgatgat gataatgtgc
    12781 tgaatcaatc ggtggacgct gttattatat ctcctggtcc agggcatcca ttagacgatc
    12841 aacagttaat gaaaatcata tcaacctatc aacacaaacc cattttaggt atttgtttag
    12901 gggctcaggc actgacttgt tactacggtg gagaagtcat taaaggcgac aaggttatgc
    12961 acggcaaagt tgatacacta aaggttatat cgcatcatca acatctgtta tatcaagata
    13021 taccagaaca gttttcaatt atgagatatc attcattaat aagtaaccct gacaattttc
    13081 cagaagaatt gaaaattact ggacgtacca aagattgtat acagtcattc gagcataaag
    13141 aaagaccgca ttatggtatt cagtaccatc ctgaatcatt tgctacagac tatggtgtca
    13201 aaataattac aaatttcatt aatctagtga aggaaggatg aaaaccatga cattactaac
    13261 aagaataaaa actgaaacta tattacttga aagcgacatt aaagagctaa tcgatatact
    13321 tatttctcct agtattggaa ctgatattaa atatgaatta cttagttcct attcggagcg
    13381 agaaatccaa caacaagaat taacatatat tgtacgtagc ttaattaata caatgtatcc
    13441 acatcaacca tgttatgaag gggctatgtg tgtgtgcggc acaggtggtg acaagtcaaa
    13501 tagtttcaac atttcaacga ctgttgcttt tgttgtagca agtgctggcg taaaagttat
    13561 aaaacatggt aataaaagta ttacctcaaa ttcaggtagt acggatttgt taaatcaaat
    13621 gaacatacaa acaacaactg ttgatgatac acctaaccaa ttaaatgaaa aagaccttgt
    13681 attcattggt gcaactgaat catatccaat catgaagtat atgcaaccag ttagaaaaat
    13741 gattggaaag cctacaatat taaaccttgt gggtccatta attaatccat atcacttaac
    13801 gtatcaaatg gtaggcgtct ttgatcctac aaagttaaag ttagttgcta aaacgattaa
    13861 agatttaggt agaaaacgtg caatcgtttt acatggtgca aatggtatgg atgaagcaac
    13921 actatctggt gataatttga tatatgaatt gactgaagat ggagaaatca aaaattacac
    13981 attaaatgcg actgattatg gtttgaaaca tgcgccgaat agtgatttta aaggcggttc
    14041 acctgaagaa aatttagcaa tctcccttaa tatcttgaat ggtaaagatc agtcaagtcg
    14101 acgtgatgtt gtcttactaa atgcgggttt aagcctttat gttgcagaga aagtggatac
    14161 catcgcagaa ggcatagaac ttgcaactac attgattgat aatggtgaag cattggaaaa
    14221 ataccatcaa atgagaggtg aataatatga cgattttatc agaaattgtt aaatataaac
    14281 agtcactttt acaaaatggc tattatcaag acaaacttaa taccttgaaa agtgtgaaga
    14341 ttcagaataa aaaatctttt ataaacgcaa ttgagaaaga accaaagcta gcaattattg
    14401 cagaaattaa atcgaagagt cctacagtta atgacttacc tgaacgagat ttatcgcaac
    14461 aaatctcaga ttatgaccaa tatggtgcaa atgccgtgtc cattttaact gatgaaaagt
    14521 actttggtgg tagttttgaa agattacaag cattgacgac aaaaacaaca ttacccgtat
    14581 tatgcaaaga ctttattata gacccgcttc aaattgatgt tgctaaacaa gctggtgcat
    14641 ctatgatttt attgatcgtt aacatcttat ctgataaaca attgaaagat ttatataact
    14701 acgctatatc gcaaaatcta gaagtgttag ttgaagtaca tgatcgccat gaattagaac
    14761 gtgcctataa ggttaatgct aaattgattg gtgtaaataa cagggactta aaacgatttg
    14821 ttacaaatgt ggaacataca aatactattt tagaaaataa aaaaacaaat cattattata
    14881 tttctgaaag tggtattcac gatgcatctg atgtaagaaa aatcttgcat agtggtatcg
    14941 atggcttact aataggtgag gcgcttatgc gttgtgacaa tctatctgaa tttttaccac
    15001 aactgaaaat gcaaaaggtg aagtcatgat gaaattgaaa ttttgtggct ttacatcaat
    15061 aaaggatgtt acagcggcca gtcaattacc tattgatgcg ataggtttca tccattatga
    15121 aaaaagtaaa aggcatcaaa caattaccca aataaaaaag ttagcgtctg ctgttccaaa
    15181 tcatatcgat aaagtatgtg tcatggtaaa tcctgattta acaacaattg aacacgtatt
    15241 aagcaatacg tcaattaaca caatacagtt acacggcaca gaatctattg attttataca
    15301 ggaaattaaa aagaaatatt caagcattaa aatcactaaa gctttagctg cagatgaaaa
    15361 cataatccaa aacataaata aatataaagg gttcgtagat ttatttatta tcgacacacc
    15421 ctcagtgtcg tatggtggta ccggtcaaac atatgactgg actattttga agcacataaa
    15481 agacatacct tatttgatag caggaggcat taactctgaa aatattcaaa cagttaatca
    15541 acttaaatta tcacatcaag gttatgatct tgcatcaggt atagaagtaa atgggcgaaa
    15601 agatatagaa aaaatgacag caattgtaaa tattgtgaaa ggagatagag acaatgaata
    15661 aacaaataca aacagaagca gatgaattag gtttctttgg tgaatatgga gggcaatatg
    15721 ttccagaaac attaatgcca gcaattattg agttgaaaaa agcttataaa gaggcaaaag
    15781 cagacccaga gtttcaaaga gaactggaat actatttatc agagtatgta ggacgcgcga
    15841 caccacttac atatgctgca tcatatactg aaagcctagg tggcgctaaa atatatttga
    15901 aacgagagga tctaaatcat acaggcgccc ataaaattaa taatgcgtta ggtcaagcgt
    15961 tgcttgctaa aagaatgggc aagaagaagc ttgttgctga aactggtgcg ggtcaacatg
    16021 gtgtagctag tgctacggtt gctgcattat ttgatatgga acttgttgtc tttatgggaa
    16081 gtgaagatat taaaagacaa caacttaatg tatttagaat ggaattactt ggtgcaaagg
    16141 ttgtggcagt tgaagatggt caagggactt tatcggatgc agttaataaa gcattgcaat
    16201 attgggtaag tcatgtagat gatacacatt atttattagg ttctgcatta ggtccagacc
    16261 cgttcccaac gattgttaga gattttcaga gtgtgattgg taaagaaata aaatcacaga
    16321 tattgaagaa agaaggtcga cttccggatg caattgtagc atgtatcggt ggtggctcaa
    16381 atgcaatcgg tacattttat ccatttatta aagatgatgt tgcattatac ggtgttgaag
    16441 ccgcaggtca aggcgatgat actgataaac atgcacttgc aattggcaaa ggatcacctg
    16501 gcgtattaca tggtactaaa atgtatttaa ttcaagatga agatgggcaa gtgcaactag
    16561 cacattctat ttcagcagga cttgattatc ctggtattgg accagaacat tcttattacc
    16621 acgacattgg tagagtaact tttgaaaatg ctagtgatac acaagcaatg aatgctttaa
    16681 tcaactttac aaaacatgaa ggtattatac ctgcaattga aagtgcacat gcactgagtt
    16741 atgttgaaag actagcgcct acgatgtcga aagaagatat tattgtagta actatttctg
    16801 gacgtggcga taaagatatg gaaacaatta gacaatatat ggtagagcga ggtcttgcaa
    16861 atgactaaat tatttatacc ttatattatg ggcaataaag atttgattga aaatgcaaca
    16921 ttgttgagtg aaaatggtgc agatataatt gaaattggag tacctttctc tgatccggtt
    16981 gctgatggtc cagttatcat ggaagcaggt caacaagcga ttaaacaagg catcacgata
    17041 gattatattt tcaatcaatt agaaaaacat ggtgatcaaa ttaagtgtaa ctatgtatta
    17101 atgacgtatt ataatattat ttgtcattat ggagaacaag cgttttttga aaaatgtcga
    17161 gatactggtg tctacggctt aattattcct gatttaccat atgaattatc gcagcgttta
    17221 aaacaacaat ttagtcacta tggcgtcaaa atcatatcgt tagttgcgat gactactgat
    17281 gacaaacgta taaaagatat cgtatcccat gcggaaggct ttatttatac tgtgacgatg
    17341 aatgcgacaa cagggcaaaa cggtgcgttt catccagaat taaaacgaaa aattgagtca
    17401 attaaagcga tagccaatgt gccagttgtc gcaggatttg gtataagaac accacaacat
    17461 gttgcagata taaaagaggt tgcagatggc attgtcattg gtagcgaaat cgttaagcga
    17521 tttaaatcta acacgcgtga ggaaatcatt aaatatttac aatctatcca acaaacattg
    17581 aataattaag tttacttgat ttaaaaaaat taggcgaata ctgtttgaaa aagtgaaaaa
    17641 cggtgaatta taaaattgaa tagaaatgat acaaaagtaa tatgagcaaa cccaaacgtt
    17701 catattactt tttttgaaat tgtattcaaa aatctaaata ttactataaa agtatacgca
    17761 attaaagcgt ttatgtttta gttttaacat taactattgt atacttattt agattagatt
    17821 tattattttt gacatttgca gaggggaaat agaaaaactg caaatacgga aatgaaatta
    17881 attaacgaga gacaaatagg agtaatgata atgaagttta caaatttaac agctaaagag
    17941 tttggtgcct ttacagatag catgccatac agtcatttca cgcaaactgt tggccactat
    18001 gagttaaagc ttgctgaagg ttatgaaaca catttagtgg gaataaaaaa caataataac
    18061 gaggtcattg cagcttgctt acttactgct gtacctgtta tgaaagtgtt caagtatttt
    18121 tattcaaatc gcggtccagt gattgattat gaaaatcaag aactcgtaca ctttttcttt
    18181 aatgaattat caaaatatgt taaaaaacat cgttgtctat acctacatat cgatccatat
    18241 ttaccatatc aatacttgaa tcatgatggc gagattacag gtaatgctgg taatgattgg
    18301 ttctttgata aaatgagtaa cttaggattt gaacatactg gattccataa aggatttgat
    18361 cctgtgctac aaattcgtta tcactcagtg ttagatttaa aagataaaac agcagatgac
    18421 atcattaaaa atatggatgg acttagaaaa agaaacacga aaaaagttaa aaagaatggt
    18481 gttaaagtaa gatttttatc tgaagaagaa ctaccaattt ttagatcatt tatggaagat
    18541 acgtcagaat caaaagcttt tgctgatcgt gatgacaaat tttactacaa tcgcttaaaa
    18601 tattacaaag accgtgtgtt agtaccttta gcgtatatca actttgatga atatattaaa
    18661 gaactaaacg aagagcgtga tattttaaat aaagatttaa ataaagcgtt aaaggatatt
    18721 gaaaaacgtc ctgaaaataa aaaagcacat aacaagcgag ataacttaca acaacaactt
    18781 gatgcaaatg agcaaaagat tgaagaaggt aaacgtctac aagaagaaca tggtaatgaa
    18841 ttacctatct ctgctggttt cttctttatc aatccatttg aagttgttta ttatgctggt
    18901 ggtacatcaa atgcattccg tcattttgcc ggaagttatg cagtgcaatg ggaaatgatt
    18961 aattatgcat taaatcatgg cattgaccgt tataatttct atggtgttag tggtaaattt
    19021 acagaagatg ctgaagatgc tggtgtagtt aaattcaaaa aaggttacaa tgctgaaatt
    19081 attgaatatg ttggtgactt tattaaacca attaataaac ctgtttacgc agcatatacc
    19141 gcacttaaaa aagttaaaga cagaattttt taggaaggga attatcaaaa catgaaattt
    19201 acagagttaa ctgttaccga atttgacaac tttgtacaaa atccatcatt ggaaagtcat
    19261 tatttccaag taaaagaaaa tatagttacc cgtgagaatg atggctttga agtagtttta
    19321 ttaggtatta aagacgacaa taacaaagta attgcagcaa gccttttctc taaaattcct
    19381 actatgggaa gttatgttta ctattcgaat cgtggtccag taatggattt ttcagattta
    19441 ggattagttg attattattt aaaagagtta gataaatatt tacagcaaca tcaatgttta
    19501 tatgttaaat tagatccgta ttggttatat catctatatg ataaagatat cgtgccattt
    19561 gaaggtcgcg agaaaaatga tgccctagta aacttgttta aatcacatgg ttacgagcat
    19621 catggcttta caactgagta tgatacatcg agccaagtac gatggatggg cgtattaaac
    19681 cttgaaggta aaacacccga aacattgaaa aagacatttg atagtcaacg taaacgtaat
    19741 attaataaag cgataaacta tggtgttaaa gtcagattcc ttgaacgtga tgagttcaat
    19801 cttttcttag atttatatcg tgaaactgaa gagcgtgctg gatttgtatc aaaaacagat
    19861 gattattttt ataactttat tgacacatat ggagataaag tattagtacc attagcatat
    19921 attgaccttg atgaatatgt gttaaagttg caacaggaat tgaatgacaa agaaaatcgt
    19981 cgtgatcaaa tgatggcgaa agaaaacaaa tcagataagc aaatgaagaa aattgcagaa
    20041 ttagataagc aaattgatca tgatcagcat gaattattga atgcaagtga attgagcaaa
    20101 acggacggcc caattctaaa ccttgcttct ggcgtttatt ttgcaaatgc atatgaagtg
    20161 aattatttct ctggtggttc atcagaaaaa tataatcaat ttatgggacc atacatgatg
    20221 cattggttta tgattaacta ttgcttcgat aatggctatg atcgttataa tttctatggt
    20281 ttatcaggtg attttacgga aaacagtgaa gattatggcg tataccgctt taaacgtgga
    20341 tttaatgtac aaatcgaaga attaataggg gatttctata aaccaattca taaagtgaaa
    20401 tattggttgt tcacaacatt ggataaatta cgtaaaaaat taaagaaata gatgtaaatg
    20461 atgttagggc ttatagttat tgatactata ggctcttttt tatatgtttt ttaataaagc
    20521 ctcgaataat gatatattaa atgacaagtt taacaagtga ttgcaataaa tataaatatg
    20581 aattgcacaa cctatttgta aatgattgag ttgagtatac tttttaattc attcaatatg
    20641 actcaaaaca agccaatatg tattatattt ttggtgaata aaccttagca taacaattta
    20701 tgtctgatta tatatgtgtg tgtttgcaag tgtgtgatat caaatgtcat atttgtctaa
    20761 ttgtaattta taaaatgcat gagcaatgat tgctgtaaca aattctagca aagtattcag
    20821 tttgtatttt agaaataaaa atgatgatag atgtatgatc gatataaaag atttaatatg
    20881 caccaaaatg ataacgtgag taattggttt aatgaaacat aacatgaatg taaatttagc
    20941 ttactgacat aaacactaca caaacataca gttatataag tccccttatg gaatttcaca
    21001 ttctagttta cataatatat attataggaa gttatatgtg tgtaacgcaa aaggtaccct
    21061 acatcataat cattatctaa tatcgtcaca taacttactt atgctataat catggtatta
    21121 tattgtttgg agtgatttga tgagatttgt ctttgatatt gatggtacgc tttgttttga
    21181 cggccgatta attgaccaga ctattattga tacattgtta caattacaac atgatggtca
    21241 tgaacttata tttgcatcag cacgtccgat tcgtgatttg ttgccagttt taccatcagt
    21301 atttcatcag cacacattaa ttggcgcaaa tggtgctatg atttcacagc aatcaaagat
    21361 ttctgttatc aaaccaattc atactgatac atatcatcat atcttcaaaa taattcaaaa
    21421 gtatgagtta gattatatta ttgatgatga ttggaattat gctgcacaac ttgacgctga
    21481 gaacgcgatt tttgagcgtt tagatccaca taagctggcc agttgtattg atgttgcaaa
    21541 tatcgacaca ccaatcaaga ttattttatt aaatatagac ccggcacaaa ttacaactat
    21601 attagacgag ctagataaat accatcaaga attggaaatg attcaccatt caaatgagta
    21661 taacattgat ataacagcgc aaaatattaa caaatatact gcattacaat atatatttga
    21721 tgcagatgtt aaatatatag catttggtaa tgaccacaat gatattgtca tgttacaaca
    21781 tgctagtagt ggctatatta taggaccatc agaagcatac acacacgcaa tattgaaact
    21841 tgataaaatc aaacacatca ataataatgc acaagctatt tgcaaagtct taaaatcata
    21901 taaataaaaa cacccctatc aaatgataat cattatcaat cgataggggc tattttaata
    21961 aaattcgtcc tcgaacattt cttcctcttc atctaatcca aataattctg ccatttctcc
    22021 atgttcaatt aacatgttta aatatgcatc gcggagttct tcttcactca tatcattaat
    22081 catttcttta agactatcaa tccacatatt tctgcgtaat tgatagtctt cttcaacttc
    22141 gtttaacatc attatatgtt tatttgctgc ttctggacta gctgtaaaga gtaatgcaat
    22201 catatgttta catatcactc gtcttccatc agcatgagga caattacata tggattttct
    22261 aggatgttcc atatcaatat aacaacgata tactttgttg ccactgccct ttacttcagc
    22321 ctcatgctgc gtttctgaaa atgattttaa gttaatgacg cattcacttt gataataatt
    22381 aaagcctctt tctatagaac gaatacttgc aatatcaagt aatcccatta atgatactcc
    22441 tttttatttt tatttttaaa taaagaaaat aaaatagata agtgtctaga ttaaaatact
    22501 tg
//
LOCUS       DANJFZ010000033        22442 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.033, whole genome shotgun sequence.
ACCESSION   DANJFZ010000033 DANJFZ010000000
VERSION     DANJFZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.033"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            105..1013
                     /gene="rocF"
                     /locus_tag="PWN45_001130"
     CDS             105..1013
                     /gene="rocF"
                     /locus_tag="PWN45_001130"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDL0622909.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            1202..2011
                     /locus_tag="PWN45_001131"
     CDS             1202..2011
                     /locus_tag="PWN45_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL0622910.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            2013..2945
                     /locus_tag="PWN45_001132"
     CDS             2013..2945
                     /locus_tag="PWN45_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDL0622911.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            2972..4327
                     /locus_tag="PWN45_001133"
     CDS             2972..4327
                     /locus_tag="PWN45_001133"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL0622912.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            4604..12040
                     /locus_tag="PWN45_001134"
                     /pseudo
     CDS             4604..12040
                     /locus_tag="PWN45_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(12359..13465)
                     /locus_tag="PWN45_001135"
     CDS             complement(12359..13465)
                     /locus_tag="PWN45_001135"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL0622913.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(13465..13899)
                     /locus_tag="PWN45_001136"
     CDS             complement(13465..13899)
                     /locus_tag="PWN45_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0622914.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMENGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(13911..16043)
                     /locus_tag="PWN45_001137"
     CDS             complement(13911..16043)
                     /locus_tag="PWN45_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0622915.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(16078..17616)
                     /locus_tag="PWN45_001138"
     CDS             complement(16078..17616)
                     /locus_tag="PWN45_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDL0622916.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            18297..20102
                     /gene="glmS"
                     /locus_tag="PWN45_001139"
     CDS             18297..20102
                     /gene="glmS"
                     /locus_tag="PWN45_001139"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL0622917.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            20334..21116
                     /locus_tag="PWN45_001140"
     CDS             20334..21116
                     /locus_tag="PWN45_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622918.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            21184..22041
                     /locus_tag="PWN45_001141"
     CDS             21184..22041
                     /locus_tag="PWN45_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0622919.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         8408 a         3560 c         3998 g         6476 t
ORIGIN      
        1 tttttatgaa gtaataagat aaacagatta tatgtgtagc tattgcttga taatggtaag
       61 cgcatacatt attctttaat tacatagagc aaagggggac gcttatgaca aagacaaaag
      121 caattgatat tataggtgca ccatcaacat ttggacaaag aaaattaggt gttgatttag
      181 gaccaacagc aattagatat gctggattaa tttcaagatt aaagcaatta gaccttgatg
      241 tatatgacaa gggggatatt aaggtacctg ctgtgaacat tgaaaaattt catagtgaac
      301 aaaaaggatt aagaaattat gatgaaatta tagatgttaa tcaaaaatta aataaagagg
      361 tttcagcatc aattgaaaat aacagatttc ctctagttct tggtggagat cattctattg
      421 cggtaggttc agtatcagca ataagtaaac attataataa tttaggtgtt atttggtatg
      481 atgcacatgg tgatttaaat atacctgaag agtcaccaag tggaaatatt catggtatgc
      541 ctctaaggat tttgacaggc gaaggtccca aagaactttt agaattaaat agtaatgtaa
      601 tcaagccaga aaacatcgta ctaattggta tgagagattt agataaaggt gaaagacaat
      661 ttatcaaaga tcataatatt aaaacattta ctatgtcaga tattgataaa ttggggataa
      721 aggaagtaat tgaaaataca atagaatatt tgaagtcacg caatgttgat ggcgttcatt
      781 tatctttaga tgttgatgct ttagatccgc ttgaaacgcc aggcactggt actagagttt
      841 tgggtggtct tagttataga gaaagccatt ttgcattgga attactgcat caatcacatt
      901 taatttcctc aatggattta gttgaagtaa atccattgat tgacagtaat aatcatactg
      961 ctgaacaagc ggtttcatta gttggaacat tttttggtga aactttatta taaataaatg
     1021 atttgtagtg tataaagtat attttgcttt ttgcactact ttttttaatt cactaaaatg
     1081 attaagagta gttataatct ttaaaataaa ttttttctat ttaaatatat gttcgtatga
     1141 cagtgatgta aatgattggt ataatgggta ttatggaaaa atattacccg gaggagatgt
     1201 tatggatttt tccaactttt ttcaaaacct cagtacgtta aaaattgtaa cgagtatcct
     1261 tgatttactg atagtttggt atgtacttta tcttctcatc acggtcttta agggaactaa
     1321 agcgatacaa ttacttaaag ggatattagt aattgttatt ggtcagcaga taagtatgat
     1381 attgaacttg actgcaacat ctaaattatt cgatatcgtt attcaatggg gggtattagc
     1441 tttaatagta atattccaac cagaaattag acgtgcgtta gaacaacttg gtagaggtag
     1501 ctttttaaaa cgctatactt ctaatacgta tagtaaagat gaagagaaat tgattcaatc
     1561 ggtttcaaag gctgtgcaat atatggctaa aagacgtata ggtgcattaa ttgtctttga
     1621 aaaagaaaca ggtcttcaag attatattga aacaggtatt gcaatggatt caaatatttc
     1681 gcaagaactt ttaattaatg tctttatacc taacacacct ttacatgatg gtgcaatgat
     1741 tattcaaggc acgaagattg cagcagcagc aagttatttg ccattgtctg atagtcctaa
     1801 gatatctaaa agtttgggta caagacatag agctgcggtt ggtatttcag aagtatctga
     1861 tgcatttacc gttattgtat ctgaagaaac tggtgatatt tcggtaacat ttgatggaaa
     1921 attacgacga gacatttcaa acgaaatttt tgaagagttg cttgctgaac attggtttgg
     1981 cacacgcttt caaaagaaag gtgtgaaata atatgctaga aagtaaatgg ggcttgagat
     2041 ttattgcctt tcttttggca ttgtttttct ttttatctgt taacaatgtt tttggaaata
     2101 tctttaacac tggtaatctt ggtcaaaagt ctagtaaaac gattcaagat gtacccgttg
     2161 aaattcttta taacactaaa gatttgcatt taacaaaagc gcctgaaaca gttaatgtga
     2221 ctatttcagg accacaatca aagataataa aaattgaaaa tccagaagat ttaagagtag
     2281 tgattgattt atcaaatgct aaagctggaa aatatcaaga gaagtatcaa gttaaagggt
     2341 tagctgatga cattcattat tctgtaaaac ctaaattagc aaatattacg cttgaaaaca
     2401 aagtaactaa aaagatgaca gttcaacctg atgtaagtca gagtgatatt gatccacttt
     2461 ataaaattac aaagcaagaa gtttcaccac aaacagttaa agtaacaggt ggagaagaac
     2521 aattgaatga tatcgcttat ttaaaagcca cttttaaaac taataaaaag attaatggtg
     2581 acacaaaaga tgtcgcagaa gtaacggctt ttgataaaaa actgaataaa ttaaatgtat
     2641 cgattcaacc taatgaagtg aatttacaag ttaaagtaga gccttttagc aaaaaggtta
     2701 aagtaaatgt taaacagaaa ggtagtttag cagatgataa agagttaagt tcgattgatt
     2761 tagaagataa agaaattgaa atcttcggta gtcgagatga cttacaaaat ataagcgaag
     2821 ttgatgcaga agtagattta gatggtattt cagaatcaac tgaaaagact gtaaaaatca
     2881 atttaccaga acatgtcact aaagcacaac caagtgaaac gaaggcttat ataaatgtaa
     2941 aataaatagc taaattaaag gagagtaaac aatgggaaaa tattttggta cagacggagt
     3001 aagaggtgtc gcaaaccaag aactaacacc tgaattggca tttaaattag gaagatacgg
     3061 tggctatgtt ctagcacata ataaaggtga aaaacaccca cgtgtacttg taggtcgcga
     3121 tactagagtt tcaggtgaaa tgttagaatc agcattaata gctggtttga tttcaattgg
     3181 tgcagaagtg atgcgattag gtattatttc aacaccaggt gttgcatatt taacacgcga
     3241 tatgggtgca gagttaggtg taatgatttc agcctctcat aatccagttg cagataatgg
     3301 tattaaattc tttggatcag atggttttaa actatcagat gaacaagaaa atgaaattga
     3361 agcattattg gatcaagaaa acccagaatt accaagacca gttggcaatg atattgtaca
     3421 ttattcagat tactttgaag gggcacaaaa atatttgagc tatttaaaat caacagtaga
     3481 tgttaacttt gaaggtttga aaattgcttt agatggtgca aatggttcaa catcatcact
     3541 agcgccattc ttatttggtg acttagaagc agatactgaa acaattggat gtagtcctga
     3601 tggatataat atcaatgaga aatgtggctc tacacatcct gaaaaattag ctgaaaaagt
     3661 agttgaaact gaaagtgatt ttgggttagc atttgacggc gatggagaca gaatcatagc
     3721 agtagatgag aatggtcaaa tcgttgacgg tgaccaaatt atgtttatta ttggtcaaga
     3781 aatgcataaa aatcaagaat tgaataatga catgattgtt tctactgtta tgagtaattt
     3841 aggtttttac aaagcgcttg aacaagaagg aattaaatct aataaaacta aagttggcga
     3901 cagatatgta gtagaagaaa tgcgtcgcgg taattataac ttaggtggag aacaatctgg
     3961 acatatcgtt atgatggatt acaatacaac tggtgatggt ttattaactg gtattcaatt
     4021 agcttctgta ataaaaatga ctggtaaatc actaagtgaa ttagctggac aaatgaaaaa
     4081 atatccacaa tcattaatta acgtacgcgt aacagataaa tatcgtgttg aagaaaatgt
     4141 tgacgttaaa gaagttatga ctaaagtaga agtagaaatg aatggagaag gtcgaatttt
     4201 agtaagacct tctggaacag aaccattagt tcgtgtcatg gttgaagcag caactgatga
     4261 agatgctgaa agatttgcac aacaaatagc tgatgtggtt caagataaaa tgggattaga
     4321 taaataaata ctgtattaca aatgagccga tgcgtatgca tcggtttttt gtgtttgtag
     4381 aaataattta tagtacaaac gtaaaatgat ataaacaaaa taaaaacaaa gtaatcaata
     4441 tgtaatataa aatacactgg tactcaatat ataatgatga taaaattaat tttaattaga
     4501 tagagttgct ttgtgttttt aacgcagatg ctactactta tcttaacagt tgattaagtg
     4561 aaatcattta acagcgagaa taatcaacca ggaggatgac ttaatgaatt tattcagaca
     4621 acaaaaattt agtatcagaa aatttaatgt cggtattttt tcagctttaa ttgccactgt
     4681 tacttttata tctactaacc cgacaacagc gtctgcagca gagcaaaatc agcctgcaca
     4741 aaatcaacca gcacaaccag ctgatgccaa tacacagcct aacgcaaatg ctggtgctca
     4801 agctaatcct acagcacagc cagctgcacc tgccaaccaa ggacaaccag cagtacaacc
     4861 agcaaaccaa ggtggacagg ctaatccagc aggaggagca gcacaaccaa atacacaacc
     4921 agctggacaa ggtaatcaag ctgatccgaa taacgctgca caagcacaac ctggaaatca
     4981 agcaacaccg gcaaaccaag caggtcaagg aaataaccaa gcaacaccta ataataatgc
     5041 aacaccggca aatcaaacac agccagcgaa tgctccagca gcagcgcaac cagcagcacc
     5101 tgtagcagca aacgcacaaa ctcaagatcc aaatgctagc aatactggtg aaggcagtat
     5161 taatacgaca ttaacatttg atgatcctgc catatcaaca gatgagaata gacaagatcc
     5221 aactgtaact gttacagata aagtaaatgg ttattcatta attaacaacg gtaagattgg
     5281 tttcgttaac tcagaattaa gacgaagcga tatgtttgat aagaataacc ctcaaaacta
     5341 tcaagctaaa ggaaacgtgg ctgcattagg tcgtgtgaat gcaaatgatt ctacagatca
     5401 tggtaacttt aacggtattt caaaaactgt aaatgtaaaa ccagattcag aattaattat
     5461 taactttact actatgcaaa cgaatagtaa gcaaggtgca acaaatttag ttattaaaga
     5521 tgctaagaaa aatactgaat tagcaactgt aaatgttgct aagactggta ctgcacattt
     5581 atttaaagta ccaactgatg ctgatcgttt agatttacaa tttattcctg acaatacagc
     5641 agttgctgat gcttcaagaa ttacaacaaa taaagatggt tataaatact attcattcat
     5701 tgataatgta ggtctattct caggatcaca tttatatgtc aaaaatagag atttagcacc
     5761 gaaagcaact aacaataaag aatatactat taatactgaa atcggtaaca atggtaattt
     5821 tggtgcttca ttaaaagcag atcaatttaa atatgaagta acattaccac aaggtgtaac
     5881 ttacgttaat aattcattaa ctacaacatt ccctaatggt aatgaagaca gtacagtatt
     5941 gaaaaatatg actgttaatt atgatcaaaa tgcaaataaa gttacattta caagccaagg
     6001 tgtgacaacg gcacgtggta cacacactaa agaagtttta ttcccagata aatctttaaa
     6061 attatcatat aaagttaatg ttgcgaatat cgatacacct aaaaatattg attttaatga
     6121 aaaattaaca tatcgtactg cttcagatgt tgtaattaat aatgcgcaac cagaagttac
     6181 actaactgca gatccatttt cagtagcggt tgaaatgaac aaagatgcgt tgcaacaaca
     6241 agtaaactca caagttgata atagtcatta cacaacagca tcaattgcag aatacaataa
     6301 acttaaacaa caagcagata ctattttaaa tgaagatgcg aatcatgtta aaactgcaaa
     6361 tcgtgcatct caagcggata ttgatggttt agtaactaaa ttacaagctg cattaattga
     6421 taatcaagca gcaattgctg aattagatac taaagctcaa gaaaaggtta cagcagcaca
     6481 acaaagtaaa aaagttacgc aagatgaagt tgcagcactt gtaactaaaa ttaacaatga
     6541 taaaaataat gcaatcgctg aaattaataa acaaactaca gcacaaggtg tcacaactga
     6601 aaaagataat ggtatcgcag tgttagaaca agatgtgatt acaccaacag ttaaacctca
     6661 agcgaaacaa gatattatcc aagcagttac aactcgtaaa caacaaatta aaaagtcaaa
     6721 tgcatcatta caagatgaaa aagatgtagc aaatgataaa attggtaaaa ttgaaacaaa
     6781 ggcaattaaa gatattgatg cagcaacaac aaatgcacaa gtagaagcca ttaaaacaaa
     6841 agcaatcaat gatattaatc aaactacacc tgctacaaca gctaaagcag cagctcttga
     6901 agaatttgac gaagttgttc aagcacaaat tgatcaagca cctttaaatc ctgatacaac
     6961 aaatgaagaa gtagcggaag ctattgaacg tattaatgca gctaaagttt ctggtgttaa
     7021 agcaattgaa gcgacaacga ctgcacaaga tttagaaaga gttaaaaacg aagaaatctc
     7081 aaaaattgaa aatattactg actctacgca aacaaaaatg gatgcctata atgaagttaa
     7141 acaagctgca acagctagaa aagctcaaaa tgctacagtt tcaaatgcaa caaatgaaga
     7201 agtagcagaa gctgatgcag cagtagatgc agctcaaaag caaggtttac atgacatcca
     7261 agttgttaaa tcaaaacagg aagttgctga tacaaaatca aaagtattag ataaaatcaa
     7321 tgcaattcaa acacaagcaa aagttaaacc tgcagctgat acggaagtag aaaacgcata
     7381 taatacacgt aaacaagaaa ttcaaaatag caatgcttca actacagaag aaaaacaagc
     7441 tgcatataca gaattagata ctaaaaagca agaagcaaga acaaatcttg atgctgcaaa
     7501 tacaaacagt gatgtaacaa cagctaaaga caatagtatt gctgcaatta atcaagttca
     7561 agctgccaca actaagaaat cggatgcaaa agcggaaatc gctcaaaaag caagtgaacg
     7621 taaaacagca attgaagcaa tgaatgattc gactactgaa gaacaacaag cagcgaaaga
     7681 caaagtggat caagcagtag ttactgcaaa cgctgatata gataatgctg cagcaaacaa
     7741 tgatgtggat aatgcaaaaa ctacaaatga agctacaatc gcagccatta cacctgatgc
     7801 aaatgttaaa ccagcagcaa aacaagcaat tgcagataaa gtacaagctc aagaaacagc
     7861 aattgatgga aataacggct caacaactga agaaaaagca gctgctaaat aacaagttca
     7921 aactgaaaaa acaacagctg atgccgcaat agatgcagca catacaaatg cggaagttga
     7981 agcggctaaa aaagcagcaa ttgctaaaat tgaagcgatt cagccagcaa caacaactaa
     8041 agataatgcg aaagaagcaa ttgctacgaa agcgaatgaa cgtaaaacag caatcgctca
     8101 aacgcaagac attactgctg aagaaattgc agcggctaat gcggacgtag ataatgctgt
     8161 gacacaagca aatagcaaca ttgaagctgc taatagtcaa aatgatgtag accaagcgaa
     8221 aacgacaggt gaaaatagta ttgatcaagt aacaccaaca gttaataaaa aagcaactgc
     8281 acgtaatgaa atcacagcaa ttttaaataa caaattgcaa gagattcaag ctacgccaga
     8341 tgcaacagat gaagaaaaac aagcagctga tgctgaagca aatactgaaa atggtaaagc
     8401 aaatcaagcc atttcagcag caactactaa cgcacaagtt gatgaagcta aagcaaatgc
     8461 agaagcagcg attaatgcgg taacaccaaa agttgtgaag aaacaagcgg ctaaagatga
     8521 aattgatcaa ttacaagcaa cgcaaacaaa tgttatcaat aatgatcaga acgctacaac
     8581 agaagaaaaa gaagcagcta ttcaacaatt agcaacagca gttacagacg cgaaaaataa
     8641 tattacagct gcaactgatg ataatggtgt agatcaggcg aaagacgctg gaaagaattc
     8701 aattcaaagc acgcaaccag caacagcggt taaatcaaat gctaaaaatg atgttgatca
     8761 agctgtgaca actcaaaatc aagcaattga taatacaact ggtgctacaa ctgaagagaa
     8821 aaatgcagca aaagatttag ttttaaaagc taaagaaaaa gcgtatcaag atatcttaaa
     8881 tgcacaaaca actaatgatg ttacgcaaat taaagatcaa gcagttgctg atattcaagg
     8941 tattactgca gatacaacaa ttaaagatgt tgcgaaagat gaattagcaa caaaagcaaa
     9001 cgaacaaaaa gcgcttattg cacaaactgc agatgcgact actgaagaaa aagaacaagc
     9061 aaatcaacaa gtagacgcac aattaacaca aggtaatcaa aatattgaaa atgcacagtc
     9121 aatcgatgat gtaaacactg caaaagataa tgcaattcaa gcaattgacc caattcaagc
     9181 atcaacagat gttaaaacga atgcaagagc ggaattgcta actgaaatgc aaaataaaat
     9241 aactgaaata cttaataata atgagactac taatgaagaa aaaggtaacg atattggacc
     9301 agttagagca gcatatgaag aaggtttaaa taatattaat gcagcaacta ctacaggtga
     9361 tgtaactact gctaaagata cagcagtaca aaaagttcaa caacttcatg caaatcctgt
     9421 taagaaacca gcaggtaaaa aagaattaga tcaagctgca gctgataaga aaacacaaat
     9481 agaacaaaca ccaaatgcat cacaacaaga aattaatgat gcaaaacaag aagttgatac
     9541 tgaattaaat caagcgaaaa caaatgtcga tcaatcatca acaaatgaat atgttgataa
     9601 tgcagttaaa gaaggaaaag ctaaaattaa tgcagttaaa acatttagtg agtacaaaaa
     9661 agatgcttta gctaaaattg aagatgcata taatgctaaa gtaaacgaag cggataactc
     9721 taacgcatcg acttcaagtg aaattgctga agcgaaacaa aaacttgctg aattaaaaca
     9781 aactgcggat caaaatgtta atcaagctac ttctaaagat gacattgaag ttcaaattca
     9841 taatgactta gataatatta acgattacac aattccaaca ggtaaaaaag aatcagctac
     9901 aacagattta tatgcttatg cagatcagaa gaaaaataat atttcagctg acactaatgc
     9961 aacacaagat gaaaagcaac aagcaattaa gcaagttgac caaaatgttc aaactgcatt
    10021 agaaagcatt aataatggtg tggataatgg tgacgttgat gatgcattaa cacaaggtaa
    10081 agcagcaatt gatgctattc aagtagatgc tactgttaaa cctaaagcga accaagctat
    10141 tgaagttaaa gcagaagata cgaaagaatc tattgatcaa agtgaccagt taactgctga
    10201 agaaaaaact gaagcattag caatgattaa acaaattaca gatcaagcta aacaaggtat
    10261 tactgatgca acaacaactg ctgaagttga aaaagcgaaa gctcaaggac ttgaagcatt
    10321 tgataacatt caaatcgact caacagaaaa acaaaaagct atcgaagaat tagaaactgc
    10381 actagaccag attgaagcag gtgtaaatgt caacgctgat gctacaactg aagaaaaaga
    10441 agcgtttacg aatgctttag aagacatttt atcaaaagca actgaagata tttctgatca
    10501 aactacaaat gcagaaatcg ctactgtcaa aaatagtgcg cttgaacaac ttaaagcaca
    10561 acgtattaat cctgaagtta agaaaaatgc tttggaagca atcagagaag tggttaacaa
    10621 gcaaatagaa ataattaaaa atgcagatgc agatgcatcg gcgaaagaaa ttgcacgtac
    10681 ggatttaggt agatattttg acagatttgc tgataaatta gataaaacac aaacaaatgc
    10741 agaagttgct gaattacaaa atgtaacgat acctgcaatt gaagcgattg tgcctcagaa
    10801 tgatccagat gctaatgata ctaataatgg tatagataat aatgatgcaa cagcaaattc
    10861 aaatgctaat gcaacaccag aaaatactgg acaacctaac gtatctgaga cgacagctaa
    10921 tggcaaagca gacgcttctc cgacaacacc aaataatagt gatgcggcaa caggtgaaac
    10981 aacagcaaca tcagcaactg atgatgcaaa tgataaaccg caagctaata acaattctag
    11041 tgtagatgca tcaacaaaca gtccgacaat ggataacgat gtaactagta aaccagaagt
    11101 agaatcaact aataacggca caactgataa acctgtaaca gaaactgata atgcaacgcc
    11161 agcagaaagc acaacaaata acaatagtac aactacagca acaaacgaga atgcaccaac
    11221 aggatcaaca gcaacggctc caacaactgc gagcacagaa gcagcatcat ctgctgatag
    11281 caaagataat gcatctgtaa atgattcaaa acaaaatgct gaagtaaata atagtgctga
    11341 atctcaatca actaatgaca aggttgcaca accaaaatct gaaaataaag ctaaggcaga
    11401 aaaagatggt agtgattcaa caaatcaaag tatggttgaa tcaacaactg aaacattgcc
    11461 ttcagcagac ataacagaac caaatgtacc ttctaataca tcaaaagata aagaagaaag
    11521 tacgacaaat caaactgatg caggacaact taagtctgaa actaacgttg catctaacga
    11581 agcagataaa tcgccaagca aagctgatac tgaagtttcg aataaaccat caacatctgc
    11641 atcttctgaa gcaaaagaaa aaatgacttc aactaatgtt agccaaaaag atgatacggc
    11701 aacagcagat actaatgata cgcaaaaatc agttggttca gctgcaaaca ataaagctac
    11761 gcaaaacgac ggtgccaatg catctccagc tacagtttca aatggaagca atagtgctaa
    11821 tcaagatatg cttaatgtaa ctaacactga cgaccaccaa gctaagacaa aatcagctca
    11881 acaaggaaaa gttaataaag ctaaacaaca agctaaaact ttaccagata ctggtatgtc
    11941 acataatgac gatttaccat atgctgaatt agctctaggt gcaggtatgg cattcttaat
    12001 tagaagattc actaagaaag accaacaaac tgaagaataa aatttattca agaatgtgaa
    12061 catctagtta tttcataggg aaatattcct tactaagtaa ccaagtctaa cagttaatat
    12121 gaatttgttt tagtaatgac caatagccat tgaataggtg gctgtggctc gaaaataaaa
    12181 caaattatta acgcaaaaat actcctttca tacataacac aagttaagtg tctatgttga
    12241 agggagtatt ttttatgttc agaaatttaa tattgttaat tcaagggaac tactacatta
    12301 ttactgattg ttaacgaata acgttggaaa cttcttgttt caaacaaagc tcaataaatt
    12361 atgaaagtga attgtacgct tcaattattt catcggctaa gccatcgtca actttagcat
    12421 attcacttaa aaacgctttt aaaccgtgtt ctttaatgta attatttttc tcaacagttt
    12481 cttggtctgc tgtatcatca tacttcaata acaatgcagc tgtatttaat aaaccagtgc
    12541 gttctaaatc tttattataa agatatgtta atggttttat aattctatct ttagggccaa
    12601 ttttacgtaa tgtacctcgt ccgacacgtg ttacttcatc agataaataa gaattgttga
    12661 aacgatctat tattttttca acataagcag cttgttccgc ttcagtaaaa tcaaattcat
    12721 tagtaatata ttgactagtt tcagctaaaa cacggcgtaa gccagcttca attgaactat
    12781 cttcaactgc atctaaaact gtagctttac ctgcaaattt accagcatac gctaaatatg
    12841 catgtcctgt attcacagtt aataatttac gctcaatata tggtgttaaa tcatcaacat
    12901 atttaatatg gtttagttct ggaccatacc atgcatcttt ttcaacaacc cattcgtaaa
    12961 atggctcaac cataacgtct aatatatttt cattcttttg taatggtaca attctatcaa
    13021 ctgctgagtt agcaaaatga atattgttac caagagggcc agtaatatca agtactgctt
    13081 ttttcaatgt atcagttgcc ataatagcat tctcacaagc aacaatatta acatggtttg
    13141 ttttttcttt taagaaaggc gcaaaagatt tagcaataat aggtagtatg ttaacaccaa
    13201 cagctgttgt aataatatca gcttctagaa ttgcttgttt caacgcttca gaaggttgca
    13261 ttgaattaat tgcatcaaca ttattcacgc gcgtcgttgt tttagactca tcagctaaaa
    13321 taacatcgta ttgatgatca tgagctaaag cattaatgat ttcttcattg acgtctgcaa
    13381 atgttacttt aacattgttg tctgcaagaa tataaccaat gaaaccacga cctatgttac
    13441 cagcaccaaa gtgaactgct ttcattatgc atctgcctcc tcgaatactt gtttaatttc
    13501 ttctgcagat tttgcttgaa cgatacgatc cacattttct tcttcgctaa atgtaattgc
    13561 aattttagat aacaagtcta aatgttcgcc atctttacca gcaattccca cgacaacttt
    13621 aactacttcg ccatcccagt caacgccttc agggatttgt aataatgtta aacctgagtg
    13681 aatcacattt gttttagctt catctgtgcc atgaggaatt gctaagccat tttccataaa
    13741 tgttgatacg acttgctcac gatctttcat tgcttgaata taagcatctg ttacagcacc
    13801 actatcaact aaggctttac ctgctttttc aattgcttca ttttggctgt taacatttac
    13861 atttaaaaag atattgtcat tactaaataa ttcgctcatt ttgtacactc ctaaataaat
    13921 tgttttttaa ttcgttctaa aaatttgttt cttaataatg tttcaagttc ctgtggattt
    13981 tccataaaag tatcaataga ttctaatttc aaacttaaaa attcggacaa gtcacttacc
    14041 agtgatgcca taaaatcatt gtcagaaata aacatggaaa tcatatattt aattgtttga
    14101 ttgtcatttt gaatactagg caatgttaac ggttcttcta aaactgtgat tagaatcata
    14161 gggtgtttaa tcatattgtc tcttaggtgt ggtattgcaa caggatatgg acttaatatc
    14221 catcctggat tgtcaatcaa tcgttgctct agtagttcag cgaatgacgt tggatcgttt
    14281 attacatcgc attgatatag ataatctgcg atatatgttt gccactgttt aacttcggta
    14341 ctaactattt tcactgaatc aagcaaagtt aaacccgaac gcatatgatg caataattgt
    14401 tctgattttt cgtcacttaa atgtttttcg aaaactgaag ttaagtatga agtttgttca
    14461 ttttcaaaag atgtgttgcc atcaacatct ttcgtttcaa catgaacatc atccttatca
    14521 atcatgtttg atgatttatc atgcgtctct tggaacgtag actttgtatt taaaaactgt
    14581 gcgacataac tgatatcact atctggtaat aatgggttta ccgttaaata gggggagtcg
    14641 atgtctaaat tcacagtaga aataatgcca tcatattgac ttaaatctag tgacttcaaa
    14701 tcgctgactg atgcttgtgt aatacgctca atctcactaa aaacttgatc tagacgagtt
    14761 gataatagac gactagttcc cataccactg ctgcaaacga ctaatatgtt taaaaatcga
    14821 ttaccttggt ttttaatcga gccaccaaag tgtaaaacta taaaagcaat ttcactctct
    14881 ggaaaaatca aatcaggcca acaatcattt aaagctaatc ttacattttc aaatagtctt
    14941 ggatatttga acttaatcat gtctgttaac ggattatagg tctcgatatt agcttgcaaa
    15001 cgattgattg caggattaag gtgtagcgtc agtccatcta ctaaagtatc caaatctgca
    15061 aatgtcatac cggaaatcat ttctactttg ttaacaaacg ctttaatttt gttttcttca
    15121 caacgattat ttaatgatgt atcattaaga ttttttcgtt tagctccacg taaatggata
    15181 gtaatgaaag caatttctgc ctgattaaac gtgacgtcat atatttgacc aagtttatca
    15241 gcaagttcgc ttgctacttt gtgttcaaat gtgttttgta cagaatcata aatatcatcg
    15301 tttaatgcga catactctcc attttttata cgtgaaatgg agagcacgat atggacagtt
    15361 aaagttaaat aacttgattc ggtaagttgg tagggtaagg cacttaggta gtccattaat
    15421 agtctttcaa cattaaaaat gcgctctaag tcaacaaagt ctttgtggat ttgatttaat
    15481 gaatgaaaga caaaatgatt ttcaattact gaataaacgc tagtactatt taagttattc
    15541 accattaatt gacttaaaaa ttcacgtttc tttgattcag tacctaccaa gtaaatgcct
    15601 tcgccacgct ttcgagataa agatagttgg tacttattta aatcaagctc taaatcatct
    15661 aacatctttg ctaaagtctg gacagaaacg ccaatttctt gcgctaaact atattgttta
    15721 actggctcct tggcttgtat caaagcgtat ataataatta ctttctgctc ttcaacagat
    15781 aagtcaatgg tttgatgttg tgcaatcgat tgcttcaaat cgtttaaatc agaatctgtg
    15841 ccagcaatgc gtagcccttt tttgtttgcg cgttctaaag ttaatgaaaa tgaagttaaa
    15901 tatgcttcaa caccttttaa ttcacggtga atagtacgag aggacaccgc aagttgctga
    15961 gcaatgtcgt gtatagtgat atattgaccg tggtacttaa tcaatagggc tatcatttct
    16021 ttttcacgtg tactcaataa catggcggta tcctccattt tttaatttat ttaattatta
    16081 tgcttgatca tcttttttta gattatttaa aagttcttca tatcttggtg aattaaggaa
    16141 attatccact gaaatatgga tggcatttgg tgtttgttta atagcacgat cagttagttt
    16201 tttctgagtg ataactaatt gagcatcttt tggcaattga ttaatcgcag tatttgtaac
    16261 tgtaatatca ttaatgcccg cctttttaaa tttattacgt aacatgcttg cacccattgc
    16321 actagaaccc atacccgcat cgcaggcaaa aataacatga tttatattat tgtaattgtg
    16381 tgcatcaaca tcttcagtgt tgtaattatc caatagcgct tcaggatcat catctgatga
    16441 agatgtttca ctaacattac cactagcatt ttcttctgta ttaacatttt tatcagaaga
    16501 tactaactta gaagcaacgc ttgatttttt ccctttagta ttttccattt gagctgtcgc
    16561 agcttctaaa tcctgctttg gttctctagt gaacttcata attaaagcag ctacaacgaa
    16621 tgatacaagt gcagcaagga agacaccgag taacatgtgc aagaattcac ctctaggtgc
    16681 atttaaacag taaactataa atgaacctgg tgacgcggga cttttaaatc caaatcctgt
    16741 tgcttgataa gttgcaacac cagtcattcc acctaaaata acagcgataa ataataaagg
    16801 acgcattaat acatatggga aataaatttc atgaatacca cctaagaagt ggataattcc
    16861 agcaccatat gacgttgctt ttgcagtgcc ttttccaaaa atcatataag caagtaagat
    16921 acctaaacct ggtccagggt tagattcaat tgtgtataaa attgattgac cagcttttgc
    16981 agcttgatct gcaccaagcg gtgtgaatac accatggtta atcgcattgt ttaaaaatac
    17041 aatttttgca ggctctacta aaatacttac aagtggaagt aggtgtgcat gtactaatgc
    17101 ttcaactgcc actgataaaa tatgcataat aaatttcata agtggtgcta aaattttaaa
    17161 tcctgcaatc gtcatgataa atcctaaaat accagcagaa aagttattaa ataacatttc
    17221 aaaaccttgc ggcgttctag gttgaatcaa ttggtcggtc ttcttcatta accaaccaac
    17281 aagtggaccc ataatcattg caccaagtaa cattggtgta tcaggtaatg caacgatgac
    17341 ccccatagtt gctgttgctg cgatgatacc accacgtaaa tcataaatta aacgaccacc
    17401 actaaatgcg atcaataatg ggattaaata agtaatcatt ggtcctgcta aagtagctaa
    17461 atctttgtta ggtaaccatc cattatctat aaaaatggcc gcgataaaac cccaagcgat
    17521 gaaagcgcca atgtttggca tgatcatact acttaagaat gatccaaatg cttgaacacg
    17581 acgaccaatt ccttttttct cttcagtttg tgacatgttt acacctcttt tttaatatat
    17641 gtgactatat atagtgtata gagaggttac ttgttactca atataaacaa aaatcaactt
    17701 tgtcaaaata aatgtgacaa aattaaataa agtgtcatca atgtgacagt atagatattt
    17761 tgaaaaagta aaacaaaaaa attgttttag gatttttaaa attttattgt gaaaatattt
    17821 gcaaaacaaa acaacaccgt tgtacaataa tgattaatgg aaagggggaa agttcggcag
    17881 tacagttaaa gcgcctgtgc aaataaatat ttgtatttga agattaaagg ttaatatatg
    17941 agtggccttt atagagtgca atatatgtat ttgtagacga ggaggatagt gatcgaatag
    18001 atcggcggat gctatcccgg atgtggctca ttcgttagct tattaagtaa aacattaggg
    18061 tgacttaatg gacaaagtta ataagatcgc cagaaattga atataaaaaa tattaatatg
    18121 gaaagtacag tgtgagcaat ttgtatagtt gtaaaaataa ctatgcttaa tttgttatgg
    18181 atgaatgcga tgatagcatg ttcctattta tattatgaaa gcagattgtc aatctaaatt
    18241 atcggcaata aatcataatt tacgcgtact attccaatat ggaggaaaat gtcgttatgt
    18301 gtggaattgt tggttatatt ggctatgata atgccaaaga attattatta aaaggtttag
    18361 aaaaattaga atacagaggt tatgactctg caggtatcgc agtagtaaat gatgataata
    18421 caactgtatt taaagaaaaa ggtcgtattg cagaattacg taaagttgct gatagtagcg
    18481 attttgatgg acctgttgga atcggtcaca cacgttgggc aacacacggt gtaccgaatc
    18541 atgaaaactc tcatccacat caatcatcaa atggccgttt tactctagtt cataacggtg
    18601 ttattgaaaa ctatgaagag ttaaaaggtg aatacttaca aggtgtatca ttcatttcag
    18661 aaacagatac agaagttatc gttcaattag ttgaatactt ttcaaatcaa ggactttcaa
    18721 ctgaagaagc atttacaaaa gttgtgtcat tattacatgg ttcatatgca ttaggtttat
    18781 tagatgctga agacaaagac acaatctatg ttgctaaaaa taaatcacca ttattattag
    18841 gtgttggtga aggtttcaat gttatcgcat cagacgcact tgcaatgtta caagtgacaa
    18901 gcgaatataa agaaatccat gaccatgaaa tcgttattgt taaaaaagat gaagttatta
    18961 ttaaagatgc agatggaaac gttgtagaac gtgattcata tattgctgaa attgatgcat
    19021 cagatgctga aaaaggtgtt tatgcacact acatgttaaa agaaattcat gaacaaccag
    19081 cagtaatgcg tcgtattatt caagaatatc aagatgcaga aggtaacttg aaaattgatc
    19141 aagacatcat caatgatgtt aaagaagcag accgcattta cgttattgca gcaggtacaa
    19201 gctaccatgc aggtttagta ggtaaagaat ttttagaaaa atgggctggc gtaccaactg
    19261 aagtacacgt tgcatcagag tttgtctaca acatgccatt attatctgaa aaaccattgt
    19321 tcgtttatat ttctcaatca ggtgaaactg cagatagccg cgccgtatta gttgaaacta
    19381 ataaattagg tcataaatca ttaacaatca ctaatgttgc aggttcaact ttatcacgtg
    19441 aagcagacca cacattgtta ttacacgcgg gtcctgaaat cgcagttgca tctacaaaag
    19501 catatactgc acaaattgca gtattatcaa tcttgtctca aatcgttgca aaagagcatg
    19561 gtcgtgaagc agatattgat ttattgagag aattagcaaa agtaacaaca gcaatagaag
    19621 caattgttga cgatgcacca attatggaac aaattgctac agatttctta gaaacaacac
    19681 gcaatgcatt ctttatcgga cgtactattg actataacgt aagtttagaa ggtgcgttaa
    19741 aacttaaaga aatttcttac attcaagcag aaggttttgc tggtggagaa cttaaacatg
    19801 gtacaattgc cttaatcgaa gaaggtacac cagttgtagg tttagcaaca caagagaaag
    19861 ttaatttatc aattcgtggt aacgttaaag aggtagtagc acgtggtgca catccatgta
    19921 ttatttctat ggagggtctt gaaaaagaag gcgacactta tgtcattcct catgtacatg
    19981 aattgttaac gccattagta tcagtggttg cattacaatt aatttcatac tatgcagcat
    20041 tacacagaga tttagatgtt gataaaccac gtaaccttgc taaatcagtt actgtggaat
    20101 aattcacttt tttagaatca atcatgtatt aaaattaaag tatatggcac ccttttagat
    20161 taatcgacta gaagggtgct tttttaggtc gacttagctt ttacttcatc ttaatttggc
    20221 agaaatgcgt aaaaatgaag tgttttattt atttaaatag tctgacaatt aagggtgtta
    20281 tgttaatatg attttatgag aagtatggag tagcaataaa ggggtgacct cgcatgttaa
    20341 ttcaattaga tcaaattggg cgaatgaagc aaggaaaaac aattttaaaa aagatttctt
    20401 ggcaaattgc taaaggtgat aaatggatat tatatgggtt gaatggtgct ggcaagacaa
    20461 cacttctaaa tattttaaat gcgtatgagc ctgcaacatc tggaactgtt aaccttttcg
    20521 gtaaaatgcc aggcaaggta gggtattctg cagagactgt acgacaacat ataggttttg
    20581 tatctcatag tttactggaa aagtttcaag agggtgaaag agtaatcgat gtggtgataa
    20641 gcggtgcctt taaatcaatt ggtgtttatc aagatattga tgatgagata cgtaatgaag
    20701 cacatcaatt acttaaatta gttggaatgt ttgctaaagc gcaacaatat attggttatt
    20761 tatctaccgg tgaaaaacaa cgagtgatga ttgcacgagc tttaatgggg caaccccagg
    20821 ttttaatttt agatgagcca gcagctggtt tagactttat tgcacgagaa tcgttgttaa
    20881 gtatacttga ctcattgtca gattcatatc caacgcttgc gatgatttat gtgacgcact
    20941 ttattgaaga aataactgct aacttttcca aaattttact gctaaaagat ggccaaagta
    21001 ttcaacaagg cgctgtagaa gacatattaa cttctgaaaa catgtcacga tttttccaga
    21061 aaaatgtagc agttcaaaga tggaataatc gattttctat ggcaatgtta gagtaaatat
    21121 tttgcaaata ataagtaata atgacaaaat ttaattaaga taaaatggac agtggagggc
    21181 aatatggata acgtaaaagc aatatttttg gacatggatg gaacaatttt acatgaaaat
    21241 aatcaagcat caacgtatac gaaagatgtc attaatcaat tgagagagaa aggatataaa
    21301 gtatttttgg caacaggacg ttcgcattct gaaatacatc aacttgtacc tcaagatttt
    21361 gcggttaatg gcatcattag ttcaaatgga acaattggag aagtagatgg agaaattatc
    21421 ttcaagcatg gtttatcatt ggctcaagtg caacaaatta ctaatttagc taagcgccaa
    21481 caaatttatt atgaggtatt tccttttgaa ggtaatagag tttctttaaa agaagatgaa
    21541 acatggatgc gagatatgat tcgtagtcaa gatcctatta atggcgtaag tcatagtgaa
    21601 tggtcttcaa gacaagatgc gcttgctggt aagatagatt gggtaactaa gtttcctgaa
    21661 ggtgaatatt caaaaattta tctattcagt tctaatttag aaaaaataac agcatttaga
    21721 gatgaattaa agcaaaatca tgtgcaacta cagattagtg tttcaaattc atcaagattt
    21781 aatgcggaaa caatggctta tcaaactgat aaaggtacag gcattaaaga aatgattgca
    21841 cattttggta ttcatcaaga agaaacgtta gttattggag atagcgacaa tgatagagca
    21901 atgtttgaat ttggtcatta tacagttgct atgaaaaatg cacgccctga aatccaagca
    21961 ttaacttcag atgtaacggc atacacgaat gaagaggatg gcgcagcaaa atatttagca
    22021 gagcattttt tagctgaata ataaaatagg tagttattta ttatttaatt tacaatagtt
    22081 gatgagtaat gtacaaagag cagtaaagtt attttctatt agaaaatgtc ttactgctct
    22141 tttgtatgct tataaatatt tgaatcatct atatttaatt ggacaaactc tatgagaata
    22201 aatattgtta aaactaataa gataggaaat tcattgattt tgaataatat ttcttgtttt
    22261 aaggtttaac tattgaattg tatacttctt tttttagtag caacagatcc gtctttaaaa
    22321 aaattcacac ctattgactg tttttgttct tgattatatt ctctatgaag tgttaatcca
    22381 ctataaaatc aaccggttcg tttacatatt tccccaatat aatttactct atagtatact
    22441 tc
//
LOCUS       DANJFZ010000034        22260 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.034, whole genome shotgun sequence.
ACCESSION   DANJFZ010000034 DANJFZ010000000
VERSION     DANJFZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.034"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(92..757)
                     /locus_tag="PWN45_001142"
     CDS             complement(92..757)
                     /locus_tag="PWN45_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0622920.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(757..1812)
                     /locus_tag="PWN45_001143"
     CDS             complement(757..1812)
                     /locus_tag="PWN45_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0622921.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1948..2622
                     /gene="hssR"
                     /locus_tag="PWN45_001144"
     CDS             1948..2622
                     /gene="hssR"
                     /locus_tag="PWN45_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDL0622922.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2615..3988
                     /gene="hssS"
                     /locus_tag="PWN45_001145"
     CDS             2615..3988
                     /gene="hssS"
                     /locus_tag="PWN45_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDL0622923.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4151..4594
                     /locus_tag="PWN45_001146"
     CDS             4151..4594
                     /locus_tag="PWN45_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0622924.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4591..5046
                     /locus_tag="PWN45_001147"
     CDS             4591..5046
                     /locus_tag="PWN45_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDL0622925.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="PWN45_001148"
     CDS             complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="PWN45_001148"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDL0622926.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(6971..8569)
                     /locus_tag="PWN45_001149"
     CDS             complement(6971..8569)
                     /locus_tag="PWN45_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL0622927.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8886..10559
                     /locus_tag="PWN45_001150"
     CDS             8886..10559
                     /locus_tag="PWN45_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL0622928.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10712..11341)
                     /locus_tag="PWN45_001151"
     CDS             complement(10712..11341)
                     /locus_tag="PWN45_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622929.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11655..12170)
                     /locus_tag="PWN45_001152"
     CDS             complement(11655..12170)
                     /locus_tag="PWN45_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622930.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            12361..13365
                     /locus_tag="PWN45_001153"
     CDS             12361..13365
                     /locus_tag="PWN45_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0622931.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(13411..13812)
                     /locus_tag="PWN45_001154"
     CDS             complement(13411..13812)
                     /locus_tag="PWN45_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0622932.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(13848..14882)
                     /locus_tag="PWN45_001155"
     CDS             complement(13848..14882)
                     /locus_tag="PWN45_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0622933.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(14893..15009)
                     /locus_tag="PWN45_001156"
     CDS             complement(14893..15009)
                     /locus_tag="PWN45_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622934.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(15114..15545)
                     /locus_tag="PWN45_001157"
     CDS             complement(15114..15545)
                     /locus_tag="PWN45_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDL0622935.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(15752..17029)
                     /locus_tag="PWN45_001158"
     CDS             complement(15752..17029)
                     /locus_tag="PWN45_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL0622936.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            17190..17813
                     /locus_tag="PWN45_001159"
     CDS             17190..17813
                     /locus_tag="PWN45_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0622937.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            17859..18803
                     /locus_tag="PWN45_001160"
     CDS             17859..18803
                     /locus_tag="PWN45_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0622938.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(18889..20331)
                     /locus_tag="PWN45_001161"
     CDS             complement(18889..20331)
                     /locus_tag="PWN45_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDL0622939.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            20514..20897
                     /locus_tag="PWN45_001162"
     CDS             20514..20897
                     /locus_tag="PWN45_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDL0622940.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         7478 a         3903 c         3206 g         7673 t
ORIGIN      
        1 cctgtttttt gtaaacccaa atcgtattct gttatctaac ttttatacta ctatgtctta
       61 aatcattaac atttagcgtc tttacaagcc actaatcagt tattttgcca tcttctaatt
      121 caatcactcg atctgcatat tcaaataatc ttcgatcgtg tgtaatcata ataccaatca
      181 tttgttgttc tttaatttgt tgacgtatca tctcaacaac ttttgttgct ctatcggcat
      241 ctaaacttgc tgtgggctca tctgctaaaa tgattttcgg attattcata aatgctctca
      301 taatcgcaac acgttgcttt tcaccgccag ataactgatg cggatataca ttcaagcgat
      361 cttctaaacc aatatttttc aaaagttgta ttgctcttgt tgaactttgt tgtttggtca
      421 ttcccgcttc ttgacctacg agtgtcaatt gctctatcac ttttaaataa ggaactaaat
      481 gtgaagattg aaaaataaaa ccaatatctt ccaatcgtaa atcactagga cgatgctgtt
      541 tatcaaacaa tggcgcatca ttgtaaagca ctgtaccact cgtttgactt aacaatccgc
      601 ctaatatcgt tagcaatgtt gttttcccag aaccagaggc accatttaaa atgacaaatt
      661 ccccttgttc cacttcaaaa ttaatacctt ttaaaacttt tgtttcagac aaaccttctc
      721 cgaaattttt gacgatatct tcaacgacta atgccattat tctgcacctc caattgcttc
      781 gataggatcc actttaaata atttgataaa tgatagtgag gcacctaaaa tcgctactaa
      841 tataaatatc cccaccatta ataaaatatt ttgcgttgtt aagtaaaaag gcatcgttac
      901 cggcatcata aatgatagtc ctactatgat gatcacagca atacctaccc caattattgt
      961 tagtgttaaa atttgtaaaa ctaacgcact caataaatgt cttgtcttaa taccaattgc
     1021 tttcaaaatg ccaatttgtg atattttttg aatcgtcata acatagaaaa atgcacttag
     1081 aacgattgct gtaatagcaa acaaactaac aatcatcata tttaacggtg cttgctctgc
     1141 ttgataactc gcaatattac ctgttaaatc tttttcactc acaacttgaa cgtcatttat
     1201 tttattaagc tcatcacgtt gttgttgtgt taaatttttc actggataaa atgccgtatt
     1261 aaccttattc aatgcattaa atccgttatc gttcaatagc acaatggaac tatgcgcata
     1321 cattgtgtcg tttaaaatac cagaaactct ataactagtt gaattatttt taaaatgtaa
     1381 tctatccccg actctaatgt tgtctgcagc taacttatca ttaatagcga cctcatgagc
     1441 atttttagga taatcacctt ttttaagttg tatgcgttgt tgtttaacgt cattcattgt
     1501 aatcacatct tgttctttat tagataattt caatgtttga atattcattt tatatggttc
     1561 ttgatgaatt acctttttaa tttgattctg ttgcgtatca ctaagttgcg atttctcaat
     1621 ttgcggctct ttcatttttt gaacaacata ttcatcatta tcaaagtgtt caaataacga
     1681 aatattctcc ctaccaagcc cttgagctaa accactaata aataacacca taatacttaa
     1741 taaaagaatg attaatgtga ttaaaatata acgaaatttg taaaacataa tctcttttat
     1801 cgctaatttc atatcgattc acttctccct atttcttctt tagcgtcagt ataaggttga
     1861 aatatgaact caatatgaac aattgttttt ttacgcaaaa atattttaca tgtataataa
     1921 atgaaaaaga taagggagtt tatagctatg gtgcaatgtc ttgttgtcga tgacgatcct
     1981 cgaatcctaa attatatagc tagccattta caaacagagc acattgatgc atacacacaa
     2041 ccaagtggtg aagctgcatt aaaactttta gaaaaacagc gtgtcgatat tgcagtggta
     2101 gatattatga tggatggtat ggacggcttt caattatgta atacattaaa aaatgattat
     2161 gatataccag ttattatgtt aacagcgcgg gatgcactta gtgacaaaga gcgtgcgttt
     2221 ataagcggta ctgacgatta tgtaaccaaa ccctttgagg ttaaggaact tatttttaga
     2281 attcgtgctg tattacgtcg atataatatc aattcaaatt cagaaatgac tattggcaac
     2341 ttaacgctaa accaatccta tttggaactc caagtatcta ataaaacgat gacgttaccg
     2401 aacaaggaat ttcaattatt atttatgctt gcagcacgtc ctaagcaaat ctttactcgt
     2461 gaacaaataa tagaaaaaat ttggggctat gattatgaag gagatgagcg aacagttgac
     2521 gttcatatta agcgactacg ccaaagatta aaaaaattaa atgccacact tacaattgaa
     2581 acagtaagag gacaaggcta taaggtggag aatcatgttt aaaacactct atgctagaat
     2641 tgcgatttat tccattacgg ttattttatt tagtgcatta ataagttttg tattaacaaa
     2701 tgtttactat cattataatt taaaagcatc taatgacgcg aaaatcatga agacgcttaa
     2761 agaagcaaga caatatgaac aatcagctaa accaacgcac attcaacaat atttcaaaca
     2821 tttagggcaa atgaattacc aaataatgac cattgatcaa aaaggtcata agacatttta
     2881 tggggagcca tttagagaag atactttatc acaaaatgct attaacaacg tattaaataa
     2941 tcaagattac catggtatta aagataaacc attcgcatta ttcgtaactg gatttttcga
     3001 caatgtaaca gataatactg ttggtattaa ctttaagaca aaagacggtt caatagcagt
     3061 atttatgcgt ccagatattg gagaaacatt tagcgagttt agaacattct tagccgtctt
     3121 gttaatgttg ttattattta tctcgatttc attagttatc gcatcaacct attcaatcat
     3181 tcgtccagta aaaaagttaa agctagcgac cgaacgctta attgatggtg attttgaaac
     3241 acctatcaaa caaacacgca aagatgaaat tggaacatta cagtatcact ttaataagat
     3301 gagagagtca ttgggacaag ttgatcaaat gaggcagcat tttgtacaaa atgtatctca
     3361 tgaaatcaaa acaccattaa cacatattca tcatttatta agcgagttac aacaaacttc
     3421 tgataaaaca ttaagacaac aatatataaa tgatatctat accattacaa cgcagctgag
     3481 cggattaaca actgaattgt tgctcttatc cgaattagat aatcaccaac atttattatt
     3541 cgatgataag attcaagtca atcaactcat taaagatatc attcgacatg aacaatttgc
     3601 tgctgatgaa aagtcgttaa tcattttagc tgacctagaa tcaatcaatt tcctaggaaa
     3661 tcaacgtttg ttacatcaag cattgagtaa tttattaata aatgctatta agtatacaga
     3721 tgttggtggt gccattgaca tcgctttgca acatagtcac aacaatatca ttttcacaat
     3781 aagtaatgat ggttcaccaa ttagtccaca agctgaagca cgtttatttg aacgttttta
     3841 taaagtgagc aaacatgata atagcaatgg tttaggtctt gcaattacaa aatccatcat
     3901 tgaattgcat catggtacaa tccaattcac acaaagtaac gagtatgtaa ccacctttac
     3961 tattacactg ccaaataatt cactttaatc tctacctcct gaaaaaacat ttctgttttt
     4021 atttcaggag gtatctttta tattgattag cgctttctag ccacttaaca tttaatttct
     4081 accattcaac aaaatttaaa tatatcgatg gtcatttatg gtttaataat tttgaaggag
     4141 gcattaacct atgatgaaac tcaatttatt tatcaatgca aatgagacgg aatcttatat
     4201 tgatatacat gcacctaaaa tgaatgataa cgttcaaagt attattaatg cagtcaatga
     4261 tttagacaaa tcacatacat tagtcggata catagacaaa gaaatccata tcattaatgt
     4321 atcagatgtt ataacattcc aagttattaa taaaaatgta acagccatta caagcaatca
     4381 aaaattcaaa ctaaaattac gactttatga actagaaaaa cagttaccac aacatttcat
     4441 tcgcatttcc aaatcagaaa tcgtcaacaa gtattacatt gaaaaattat tattagagcc
     4501 aaatggactt atacgtatgt atcttaaaga tgcacactac acctattctt ctagacgtta
     4561 tttaaaatct ataaaggagc gcttatcaat atgaaaaatt tgaaaaactc attatttatt
     4621 agcttaatca ttggtttgtc cctttcactg ttctttagta tgttatttgc agacggcaaa
     4681 tattatccac tgaacccaca atcaactatt ggcatattgt attatacgca tttcacagaa
     4741 actaccgtaa tgttgatttc tatcatttta tggttactca taggcgtcgt ctttttcctt
     4801 ggagatttta tctttaaata cacagattgg agcattacta aagcaactat aatgcatttc
     4861 ataacaacgt atgtcggatt cttgccttta gcaatgcttg ccggttggtt tccactaaca
     4921 gtgcattatc taatcatatt cacaattatc ttcattgtgg tttatgtatt aatttggata
     4981 attcaattct ttaaaaacaa gaactacgta gacactatta acaagcaact gaaacaatta
     5041 aaatgatttt tattaaccac taaaaaacgg taatgtctat cacattaaaa aagacattac
     5101 cgtttcgttt attatttaac ttgtaaatac ttagttactt cttcattaat tttatgatat
     5161 aaatcctcat gatctgttaa gcgataacca aatgacggca ccatttcttt aatctttggt
     5221 tcccatcctt tgaattcatc acgatagcat ctctgtaaaa catcaaacat aatatctaca
     5281 gctgttgacg caccaggtga tgcaccaagt aatgcagcta atgtgccatc atctgacgta
     5341 ataacttcag taccaaattg taagttacct ttagaatcct cagtatcctt gattacttgg
     5401 acacgttgcc ctgctgtaat cacttcccaa tcttcatttt tagcatttgg gaaaaagact
     5461 cttaaatcat ccatacgctc atcattagat aacatcaatt gtgacactaa atacttcgta
     5521 agactcattt ctttgatacc tgcagataac atcgtcacga tattatttgg tttaaccgat
     5581 ttaattaaat ccatatgtga acctgttttt aaaaatttag gtgagaaacc tgcaaatgga
     5641 ccaaataaca atgaacgctt gccgtctaca aaacgtgtat ctaagtgcgg cactgacatt
     5701 ggtggcgcac ccactgctgc ttttccgtac actttagcat gatgacgatc aatcacttct
     5761 tgatttgtac agcgcaggaa taatccactt accgggaaac caccaatatg ttttgattgt
     5821 ttaatcccag tcttttggag taatggtaaa ctcgcaccgc cagcaccaat aaatacaaaa
     5881 tcagattcat atgttgtcgt ttcattagtt tctaaatctt taactttaac tagccatacc
     5941 ccagatttct gtttcttgat gtctaataca ttctgcttat attgaattcc cacaccacgt
     6001 tgctctaaat tatcaaataa ctttgcagtt aacgcaccaa agttaacatc tgtaccagtt
     6061 tcatcataag ttaaagcaat tggttcatca gacttacgac cttcaatcat taacggtacc
     6121 caagatttca tttcttcttc atcttgcgat aatttcattt tttcgaataa aatacttttc
     6181 tttaacgttg caacacgact ttttataaaa gctacattat cttcgccaat gacaaaactc
     6241 atgtgtggca ccgcttgaat aaagcgatct gggttatcta attgtcctgt tttaactaaa
     6301 tatgcccaaa actgttttga aatttggtac tgctcattta tcttaattgc tttactacaa
     6361 tcaactgtgc catccttacc ttcttttgta tagttcaact cgcaaagtgc tgaatgccct
     6421 gtaccggcat tattccatac atttgaactc tcttcgccag gttgtgctaa tttttcaaac
     6481 actttaatat ttttctcagg tgataattct ttaagtaatg ttcctaatgt tgcactcata
     6541 ataccgccac caattaagat gacatctgtt ttgctatgtt gtgttgtcat aacaaataca
     6601 gtcccccttt ttaaaatgaa taatggaacg ttcgagaaat acgacatatt aataccactt
     6661 taaatcaata agtgtctcaa taggttccca tggtgatgct ctattactat ctactatgta
     6721 tattaatatt ttataataac gaaactactt taattatata ctttatgtgc gatagcacaa
     6781 agacaaaacc acaaacctta acagctatca accaatttaa tttgattgct gattactcac
     6841 tattcatttt aacataaagc gctaccatta ttataatgtc aaacttcttt cattaatgat
     6901 ccataaaaaa aagatgagat gttagaaaac atctcacctt ctgtatattt atattttgaa
     6961 atacgttttt ttataataat gacaagatga aagtccaaat acagatgaat attagtaaac
     7021 atacactgta tttcaatgtc atttttaata attctgattc cttaccaact tgttttactg
     7081 ctgcagttgc aattgcaatt gattgtggtg aaatcaattt tgccgctaca ccaccaactg
     7141 tattagcaga tacaagaagt gaaccacttg ttccaatttg ttgtgcaaca gaagcttgaa
     7201 ttggtgcaaa taatgagttg ttatttacaa ctgatcctgt cataaacaca cctatccaac
     7261 ctaaaattgg tgatagaact gggaagacat tacctgcttt agcaatacct tgacccattg
     7321 ctgcacttaa accaccataa gttgtgattt tagaaattgc taagatgaaa caaattgtaa
     7381 gaactggtaa ccacaactct ttaaatgtaa cgccgaataa tctacctgca tctttaaagt
     7441 taaccttttt agacattaaa attgtaataa taatagttaa taaaatagct gtacctgttt
     7501 gtccaataat atttaaagtc aatactaatg gtttatgcgt aacttcgctg attgttccag
     7561 gtaagttgaa tttaaatact aatgatgata aagcaccatt tggtaagaat aagtttttaa
     7621 agaatggcgc actccaaatc attacaatga ctgttaatac aatgaatggg ctccatgcat
     7681 gtaatactgc ttttgcagaa tgtgcttttg caggttcaat ttcttcatct ttattaacac
     7741 gataaatgtg ttttggttgg aattttttag aaaatactgc taatgctaac attgttaata
     7801 acggtggaat aatatctgct aattcaggtc cactgaatac agttaataat ccttgagtaa
     7861 atgtataagt gattgaaact actaaaattg ctggtaatgt ttctttaaca cctctaaaac
     7921 catcaataat aaagataagt aagaatggaa taatgaaatt tatgattgcc aatgttaaag
     7981 ttgctgattg agaaacacct aatactgaaa catctccagg taatttcaac gtttctacaa
     8041 cacctacagg gataccaatc gcaccaaaag caccagacgc tgcatttgcg actaaacata
     8101 acatcgcagc ttttaatgga ttaaatccta attgtgttaa taaaagtgca caaattgcaa
     8161 ttggtacacc aaatcctgct gcaccttcta aaaatgcgtt gaatgcaaat ccaataagta
     8221 aaacttgaat acgttggtct tgtgaaatat ttgtaatact atcttgaatt gttaaaaatt
     8281 gtccagattc aacagtaatt ttgtataaca atactgccat cataacgata tagccaatcg
     8341 gaatgatacc ttggaagaaa ccttcgacta ctgcaccaga agcgatacca actggcaatt
     8401 tgaaaaatgg tattgcaatt agtaatgtta caacaagtgt tgtaatagct gcataaatac
     8461 ctttcatttt aaaaacagtt aaacatagta aaaacagtac aatcggtatt gctgcaatta
     8521 aagacgataa taataaatta tcaaatgggt taaacgtatt tactaacata ggctgactct
     8581 tctttccata aagtagtagt gtaattgctt cattaaaatc atgctctggc tatagcattt
     8641 tgttatcgat aacattttac agaacagtga gattaatgtg atttttttca caattaaaat
     8701 ataccatttt ttcagactat taacaatatc actttcctat ttaattgttt tatttttcat
     8761 tttaaatctg ttcatcaaaa tgaattgtac ttaatgtaat gaagtaactt atactattca
     8821 tgaattttac ataagaccgt gctaaaataa ggaagatact acgatgagga atggaagtta
     8881 tagcaatgat taaacagata aatgtttcta atatgcaaaa gtttgaatct caattaatga
     8941 aagctcagtc tgaaggttat acacatgtcg ttccatatgc caatgagata atgatttatc
     9001 aatcgatgtt agatgcggtt caattatatc ctaagtctat cgtggttgat tatactgtgg
     9061 atggacaata caaaaatgat tgtcactatt tcggtcaatc atcgatcaac attgccgatt
     9121 gggctcaaaa caataattat tatcctaacc tgatatatgc gattcaacag actcttgatt
     9181 taatacatta ttactctgta gaaacgattt tcgatttagc tttattgaca cttctgaaag
     9241 gtgatttatc aatcgatggt catgtcgttt ttgattttaa agcccctata gcaacaagtg
     9301 cttcaatatg ggaaactatt aaaactattg aagattttga tatgatgtct cagttttatt
     9361 tgaataaaat ggcttatatt gatcatcatc ctataccttt tcgcaacctt tttatcgagg
     9421 attcagaaca acttaattcg ccagataatt ggttgtattc taccaagttt atgctaccta
     9481 aatggttata taaaatagca aagcaacgtg ctgataacaa acaattacaa aacttcggcc
     9541 tgtatacgaa gcaaccaaac gttttaaaag atcacatcgt ttttatcggt gatcatcatc
     9601 aatatatagg taattcaaaa tatttattta cttacttcgt taaacataat ccaatgactg
     9661 cctgttattt tgttactgac gatcgtcgcg gaccacattt catatcacct aagtctgaaa
     9721 aagctgacga attgattaac agcgcacgcg tagttatagt tgaaaatgat atacctgaaa
     9781 cgttacaacc aaacggtact ttaattcaat tacatcaagg cacaccaata atgcaattat
     9841 ttttagatag taaagagcct attaaaaata tagaaacacc tttttatcgt gctaaaaggt
     9901 ataatcgctg gttgcaattc gattatgtta tacattctgc tgatgatatt agccactttt
     9961 atcaaacggc ttttcctagt caccaagcaa atgtattggc ttatggtaat cctaaacacc
    10021 aatatttact acaaaagagt aacgaaagta ctactcaaca acagtataag aaatcgttta
    10081 aaataaatga tcaaaaacca gttttattat acgcacctat tggattagtg agtgcgcaac
    10141 aattaccact atcagatgct ttattcaaag cctatcatgt tgtggttcag ggtgttgatg
    10201 aagcgatctt accagaagag gcgcttgttg caccaaagta tcttagtgct caagacttaa
    10261 ttctaatgtc tgatgtcgtt atcactgatt actcaaatat aatatttgat gcgatggcta
    10321 tagacaaaac agtagcatta tacactccaa atcacagtca atacattgag tctcaaggtg
    10381 ttaacgaaga tatttggcgt catctatcaa aaatttggta tacagaccga caattattaa
    10441 ttaataattt aatttcccag gcaataccag tgattaaata tccacaaata caacataaag
    10501 aacaaccatt agaatctatt tcacaactta tattgtctaa gatgacatct aatcaatagt
    10561 gtttaaattt ctcagtggct gtgaatgagg tttaaaagta ctataaaacg taaactttga
    10621 tactttaaaa tacgcaaaaa acggtaaacc ctaattcata ttatagagtt taccgtttta
    10681 ttttttaact tgcatcatag ttatattaac attattgttg gtagtttgga tcagtaacca
    10741 ttgcttgtcc agtataatca accgttacaa ttgaatattt tccatttgca tttgggtctt
    10801 taaaactaaa cacatactta tagttgccat tatgttcttc aatagaataa tcattataca
    10861 ctttattatt actaccaaat ttatttgctt cattattagc cgcatttaaa gctgtttgga
    10921 aatttggcaa ttgctgtaaa gcttgatttt tatttccatt aaacggataa atttgacgtg
    10981 caaccggcgc ggcattttgt ccataatatg gtgcaacgta acttgatttt tgattattat
    11041 tcgcttggtt attacttgat tggttattat ttgtttggtt ttggtcattg tttgttgcat
    11101 ttgaattaga ttgttgctgg ttatcgtttg cactattatc tttattatct ttgtttacgt
    11161 ctttactatc atctttatta tctttcttat ctttagatga atcatttgtt tttttatctt
    11221 gttgttcagt tttcgcttta tcatcttttt ctttattacc gtctttttgt tggtcactat
    11281 cttgaccaca tgcagctaaa aataatgata atgctagtaa ccctgtaact aatcttttca
    11341 tacatatctc ctcctataat tcgatattca ttgaataatc ttgaaataca tatctaccat
    11401 gtgtatcttt tcatggcttg ttaccaaaga cgtcaacatt aatatgatct gaattatcac
    11461 aatatccata actacaatct aaggttcttt ttattatacc ctaatttttg ttcattatta
    11521 tttaattttt gtgaatttta tgttttctat aaatttaatt attttacttt aacaattcat
    11581 tacgcattta gcatttcaag gtatacacaa tatttattac tatgatttca ttttatctgc
    11641 tgcaaaaaca atcattataa ctctttttcc ataattaaat ctgtatccgt tacatcacct
    11701 gtttgaaaat gatgttcacc aaccacttta aatccatgac gtttataaaa tgcttgagca
    11761 cgaggattat gctcccaaac tcctagccaa attttatgtt tattatgttc ttgagcaatt
    11821 ttttcggcca attctatcaa ttgtgaacct cttccgccac cttgaaagtc tttcaaaaaa
    11881 tatatgcgct gcacttctaa ataggtctcc cccatttctt cagtttgagc actattaata
    11941 ttcatcttta tataaccaac attcgcacca tcttcttgat aaaaataatg aaatgaatct
    12001 acatggttaa tctcttgtgt aaatttctct acagtataat tgtctttaaa aaattgatca
    12061 aaatctttgt catcatagta agaaccaaac gtgtcataaa atgttctagt tgctaattca
    12121 actaattcac tagcattttg ttctgaaatt tctttgatta tcccagccat ataaatcctc
    12181 caataaacag tgatcgaatc aaaatattac ttatgttatt tttcagccaa aactatttaa
    12241 aaatacatta acacaaatca attacaaatt gtattgattg tgtgtaacat caataaatga
    12301 tacatttatt ccagtaaaat ggccgtattt tcaaaagaga aaaagagagg atgtatcgtt
    12361 gtgatagaaa catttaaagc gtttgtaatt gataaagatg agagtggtaa agtgacacca
    12421 actttcaaac aattatcgcc tactgattta cctaaaggag atgtgctgat taaagtacat
    12481 tactctggta taaattataa agatgcttta gcgactcaag atcataatgc agtcgtaaaa
    12541 tcgtatccta tgattccagg aatagattta gctggaacaa ttgttgaatc cgaagcacca
    12601 ggctttgaaa aaggagaaca agtaattgta acgagttatg acctaggtgt cagccattat
    12661 ggcggtttta gtgaatatgc gcgtgtaaaa tcagaatgga ttatcaagct tcctgatact
    12721 ttaacattag aagaatcaat gatatatggc acagctggtt atactgccgg tttagcaatt
    12781 gaaagacttg aaaaagttgg aatgaatatt gaagatggtc ctgtactcgt tcgcggtgct
    12841 tcaggtggtg tcggtacttt agcagtactc atgcttaatg aacttggtta taaagttatc
    12901 gcaagtacag gtaaacaaga tgttagcgat caattacttg aacttggtgc caaagaagtt
    12961 atcgatcgac ttcctgttga agatgatcat aaaaagccac tcgcatcatc aacttggcaa
    13021 gcttgtgtag accctgttgg tggcgaaggt attaattatg ttacaaagcg tttaaatcat
    13081 agtgggtcaa ttgcagttat tggtatgact gccggtaata cttatactaa ttctgtattc
    13141 cctcacattt taagaggtgt aaacatttta ggaattgact cggtatttac tgctatgaaa
    13201 ttaagacagc gcgtttggcg tcgtctcgca aaagatttaa tgcctgaaaa tttacatgag
    13261 atcaagcaag ttattacatt tgatgaactt ccagaacaac ttaacaaagt aattaaacat
    13321 gaaaataaag ggcgcattgt tatcgatttc ggtgtagata aatagtattc atgaaaaaga
    13381 catcccgtta tgcgagatgt cttttttaat ttagtatttg atatacatac cgcctgaatc
    13441 tggttcggta ggtataaatc caaattttgt atataattta tccgctgggt agtctgcaat
    13501 cagactaacg tatgtactct caacagccac acctttaata tattgcataa tatgctccat
    13561 aattagactg ccgtaacctt gaccttggta acttttcaaa actgcaatat caacaatttg
    13621 aaaaacagtt ccgccatcgc caatcactct acccatacca attaaccgat ctttatcata
    13681 caaggttact gtaaataagg cattaggtaa tcctttttca gctgcttcgc gcgtctttgg
    13741 actcatacct gcgttaatcc ttaatgcgca ataatcctcg caagtcggaa tatcatatgt
    13801 cactttaacc attatttacc ccacttttca tcacacaata tatcaaccta gtataaatgt
    13861 ttatttacaa tagtcttatt cgcttcttta aacacttcat gatgacttga aacataaccc
    13921 tctgcattcg catctggttg gatatatgtt ttagcaaggt tcgctgcatt tgcaccatca
    13981 ctaaatgcac ttgcaattag atgtgatttt gcatcatgat aaacaatatc tccacacgca
    14041 tagataccag gtatactagt tgtcgtatta ccaaatcctt taacacgaca atcatcatgc
    14101 atatctagct ttgaagatgt ttcactcaat aatgtattac aacgatcaaa cccatgacta
    14161 ataatgacat cgtcaaattt aactgtatgc ctatcgccac tttcaacatg ttccaaaaca
    14221 acttcactta tatgcgtttc atcatcattg ccgaccaagt atttaatacg tgtttttggg
    14281 catagtttca catttaaatc tgtcaccaac gttttcatcg cttcatgacc acttacatct
    14341 tcttttcgat aaacaactgt cacgctttta gcaatcttgg caatatcatg cgcccaatct
    14401 aatgctgtat ttcctccacc tgatattaat acatctttat ctttgaaacg tctgtaactt
    14461 tgtacaacat aatgtaaatt agttaattga tatctctcta cacctttaac atctaattgt
    14521 tttggattaa taatacccgc accaattgca atgataactg ctttcgatgt atatatttct
    14581 cccgcttctg tttcaacttc gaaatgacgt tctgcctttt tcctaatatc taccacacgt
    14641 tcattcaaat gaacttccgg tttaaaatat aatccttgct taattgtatc ttttaaaatt
    14701 tcatgacaag gttttggcgc aatgccgcca atatcccaaa taattttttc agggtaaatt
    14761 ctcatcttac cccctaattc agattgaaca tctatcaatc ttacagacat atctcgcaat
    14821 ccagcataaa agcttgcata caaaccagac ggaccgccac caatgattgt aacatctttc
    14881 attatgtgcc tcctatgact ctctatattc atttctttca ttaacgtgct caaattgata
    14941 attattatca tttaaagcca ttatactatt aatatttata ttgttaaaat aaatcgcata
    15001 gttagccatg aattatcaat aactatccca tcacaatata aaaacacacc tttcaaagta
    15061 agaaacaaca atgttccttt gaaagatgtg tatatttttt agttctagtt atattatttt
    15121 ttaaaagact catcacgtgg ttctttaaga attgcttgtc ttaaaaggaa aaatagcaac
    15181 aataaacctg caagcatacc tgtgtgccca atacctgcaa agcctgcaaa tgcttctgga
    15241 gagtatgatt taccagtgac ttggaagaat ccttttgtca ccatcatacc tatagtaacg
    15301 ataacaccta tgttatatac atagaagaac cagttaaata agtagtaact tgataattta
    15361 aataacttct ccaatggtaa taatattaaa aacataaaca tacctaaaat aagtgtatgt
    15421 gtgtgtacaa gtgataattg tgttgtacca gtaaaatcat atgcttttgt tagctctcta
    15481 tagaaaaagc cactaaataa acctatgatc atataaaaaa gaaatgaata taataaccta
    15541 cgcattttga atcctccctc accttttcat tatatccgat tatacctatt ataacagtga
    15601 caaaaaagcg aagatatgac acaactgaat ttggtttagc acatagtatg acagctaaaa
    15661 taactttaca tcaagcgcta tacaaataaa atagctatac catgacgctc gctacaaacg
    15721 tcttgtatag ctattatttc tatttattaa attatgacgg cacttcagta cgttttgtgc
    15781 tatgttttct aacaatagct gaaactaaac ttgatacagc gccaaaaatc ataaacccta
    15841 taaacatcca cattgtacta tttaattcaa tatgatgatt caagtataat agttctctta
    15901 cacttgttgc atagtgtgtg aatggattcc aatcatatac atatttttga taactttcag
    15961 gcaacatttg tttaggtaac gttacaagtt gcatactaaa gaacattaaa atgaagaaaa
    16021 ttggaactga cttcatacct agccatacca taacacctaa aataaggccc acgaaaccaa
    16081 gaatcgcaaa tgctacaaaa attgcgatac gatttggatg gtcaaaatca aatctttgaa
    16141 cgccttgcat gaaataaata taaacaaagc tacctgcaaa tgctgcaaca actgcaaata
    16201 tcatctgtcc aattgaagca atgatacgat gttgcacgac gatattgtta ctagttctaa
    16261 atgcaaagaa caataagata gacgttacga ttgaacctat ccaaattggc ataaacatta
    16321 ggaatggtgc attaccacct gcttggtgat ctttaacttt attaagtttt tcattatcca
    16381 cttttactgg attcgtcaaa ccattgatgt ccgcggcatt gactttaaca ttttgactcg
    16441 ttaatgtttc caaactttgc ttcgtaattt gagcattaat attatcaccc ataccagtta
    16501 acacagctga tgcaatttgt gaaccttgta agcttgatcc ttcacttaca atcgttttaa
    16561 atttagcctg cttaacctct acttgttgat tccccatttt ttgtttcatt tgttgaacca
    16621 cttgtggcgg aatttcacct gaagcaactt tttgttgcat ttcttctttt ttactatcca
    16681 taactacttt ttgtgtttta ctcatagcat cttttgaaaa gtctttatca ataatagcta
    16741 ctccaaagat tttttgatct ttcaaatctt tttcaaggtc tttttcacta tcaaccttaa
    16801 cccatttaat tttattagaa tcactatcta ataatttatc ctctaatttt ttaccaatgt
    16861 taacattttt atcttgaatc gttgtacctt tatcctcgtt taatatacca attggtaaat
    16921 cttttggttt aggattatat gcaggataaa aagctaaaga aaagattacc aagataatca
    16981 tagttgctat tggtgcaatc cataataatt tatttttaaa tatattcatt ttattaatta
    17041 cctccttatt agacaatgtg ttgaatattt cgcatgttgt ctattataat atgtaagtgg
    17101 gaataaacaa tacacaatgt tcatcaatac gttcattaat agacaaatac acccacattg
    17161 ttcaataaca caaggaggtt gatttatttt tgaaagaaga taggcgaatt agaaagacaa
    17221 agtcctccat taaacaagcg ttcactaaat tactacaaga aaaagattta gaaaagatta
    17281 ccattcgcga cataacaaca cgcgctgata tcaatagagg gacattttac ttacattacg
    17341 aggataaata tatgttactc gcagatatgg aagatgagta tatttcagaa ctaacgacat
    17401 atactcaatt tgatttgtta cgcggttctt caattgaaga cattgcgaat acttttgtaa
    17461 ataatatact caaaaatatt ttccaacata ttcatgataa tttagagttt tatcatacta
    17521 ttttacaatt ggaacgcacg agtcaattag aattgaaaat caacgaacat attaaaaata
    17581 atatgcaacg ttacattagt atcaatcatt ctatcggagg cgttccagag atgtattttt
    17641 acagttacgt ttccggagca acaatttcaa ttattaaata ctgggtaatg gacaaacagc
    17701 ccatttcagt tgatgaatta gctaaacatg tgcataatat tatttttaat ggtccattaa
    17761 gaataatggc agaaaaccgt ttgcataaat caaaccttga ttcactaact taagtaatac
    17821 tcgcttttcc taaatccttt agaaaggaac tatatatcat gattacttca ttcagacatt
    17881 ctgaagatat agataaacat attataaaaa caccattaga tcatacagcg tcatggatta
    17941 atgtagtaga accagaccga gaagaaattg aaaatcttat ggaacaatat aatatacctg
    18001 aagactttat acgtgaccct ttggactcag aagaaagttc ccgtattgaa tatgacgaag
    18061 atactggtta ctcattaatc attattgatt tacctatcgt caattcaact aatcgtagcg
    18121 ttctatcttt tgtaacgatt ccattaggca ttattattgg caacggtatt atcgtaacag
    18181 tttgtgacgc tgaaaatgaa tttttagaaa atttacctaa gcgtgatatt aatttaaaat
    18241 ttcacagcag atttgcatta gaaattttaa ctactattgc cgaccattat aatcgtaact
    18301 tacgattact taataaaagt agaattcgta ttgagaaaga actaaaaaat aacattacta
    18361 acaaacaact tttcaaatta atggaagttg aaaaaagttt agtatacttt ttagctgcct
    18421 taaaaggtaa cgatacaatt attaaaaagt tattccgttt acctgcaatt aaacgttttg
    18481 aagaagatga agaattacta gaagatttaa tcattgaaaa taaccaagcc atcgaaacaa
    18541 cagaattaca tcaacgtatc ttagaaagta tcacaacatc atacgcttcc ttattatcta
    18601 atgatatgaa tacgattatg aagacattaa cacttttcac ggtactatta acgttaccaa
    18661 cactcgtatt tagtttcttc ggtatgaatg tgccgttgcc aattgatgac catagttata
    18721 tatcttggat tattgtggta ggtatttcat taattcttgt cgtaattgtt agtattttct
    18781 tatggcgtaa acagaaatta taactaaatt aaaaggtatg acagcttaaa gtttcatcct
    18841 attgcatttg caatgactga tactttgatt actgtcatac ctcttttttt attctacaac
    18901 ttctttattt gcttttcttt tagataaaat taaagtaact gttatagcaa tgatgaatga
    18961 tattgtcata ccaacaaagt agtgtaacca tcctgcatgt actggattga ttgaaataaa
    19021 tcctggcaat ccagcagttc ctaatgcgat tgctttaacc ttgaagaaag caatatatgc
    19081 tgaaccaata cctgatccaa cgatagcgcc aataaatgga tatcttagtt ttaagttaac
    19141 accaaacata gccggttctg taataccaag taatgctgaa atacctgcgg cagatgccac
    19201 accttttaac ttcttatttt gtttaataat aaagaacgct gcaattgctg caccaccttg
    19261 tgcaacatta gacattgtcg caatcgggaa tataaatgat ccacccgttt tagtggcatc
    19321 tgcaattaat gtcgtttcta cagctataaa gctatgatgc atacctgtaa taacaatcgg
    19381 agcatacaat aatccgaata ttaatccacc aattgcacca ccaaattcat aaagccatgt
    19441 taaaccatct gataaccagt aacctaattg tcgagtgatt ggacctacaa atgaaaatgt
    19501 tagaaatgct gttataaaaa tagataacaa tggtgttaac aaattatcta acaccgttgg
    19561 aataactttg cgtaaccctt tttcaattga ggctaagata taagctgcta caagcatagg
    19621 taacacttgt ccttgataac ctacttgatt aatatgcaaa ccaaaaacat cccaatatgg
    19681 aatagcctta cctgcttcaa ctgcttttgg gaaatcgtat gcgctcatta gcgatggatg
    19741 aactagtatc atacctaatg cagcacctaa aaatggattg ccaccaaagc gttttgctgc
    19801 actaaatcca attaaaattg gtaataatgt aaatggtgca ttcgcaaaaa catttatcat
    19861 ttcagctaat ccagcaaatt gactatatac atctatcaat gatttacctg aaaagaataa
    19921 atctttcgca gtcaaaatgt tatttaaccc cattaataaa ccaccagcaa cgatggctgg
    19981 tataatcgga acaaagatat ctgaaagcat ttttacaaat cgctgtaacg gattcatatt
    20041 tttagcagat tgtgctttga cttccgaagt ggttgatgct tcttttccag ttaatttttc
    20101 cagttcacta aatactttat tgactgtacc agacccaata ataatttggt attgtccccc
    20161 agtagaaaac gtccctttaa ctacatccat attgtttagc gcctcttcat ttactaaact
    20221 ttcatcattt aaaactaatc gtagtctcgt tgcacaatgc gccattgcat ctaaattctc
    20281 ttctccgcct atcgcgttca aaatttcttc tgcggattgc ttataattca tatatttagc
    20341 ctcctaatta gaaccggttc catttattcc actttattgt aaccggttcc aaaaatattt
    20401 gcaacactct attatcattt tatataaaca cctacaccat ttttgatagc gcattcattt
    20461 ttattgctat actacatgca tgtatattaa cattaattat cggaggtaat tttatgaaag
    20521 aacgtggcta tatcgaacag ctttggcgtg aggaaaaata tcatgtattg ttacacagtc
    20581 aacagtctta ccaaatgata aggaatgctt taaaaacgga tttatcactt catcaagttc
    20641 aacaaatgat tgatgttgct ttgttaattg aaccgagtat aggaagtgta tgtaatgctt
    20701 ttgatcatat gtggggatat ttaaaaaaat gtgcgaatga agaagaaaga caacaatcaa
    20761 aactacttaa agctgatttt attaatggta aaatcgacac ccaaacttta ttagattttt
    20821 tagcagaact tgctaataaa tatgatgttc aatatttact acaaagtcgt gttttaaaca
    20881 caaaaagaaa gagatagatt gaaactttag ttgtaacgga caaatagtag tcattggagt
    20941 tcagcaattc atactttgta ctaaagttta ctataatcta tctctcaagg tttaaatgta
    21001 catatctata ttaaaatgcg tttcatgatg ttagaagata ccatacacat ttaattagtt
    21061 acagcataaa tcaatttata cccttaatta ttacattgtt tatattttaa attcggggga
    21121 gtaattttaa gtaatatctt gttgctgcta attctattgt taataaattg atattagatg
    21181 tagaaattga gtgtgaaagt taataataga tgatttagct agtaacataa ttaattcata
    21241 tatagtcaaa aagttctgtt agatactgtt cttgatgaat attctttgat gttaggtgtt
    21301 agctattcta gatgagtttc ttagatgtgg atgcctgtct tgatgaagtt atctatgatg
    21361 ttaatataat acttgatgtt gattcttaga tgttagtgcg atttcaagat gctgattctt
    21421 tgttgttgca ctatcttggt gacgtttcta gttgtttata gttatcgcat ctcttgatgt
    21481 aaaatctaag atgtttatgc atttctagat gttaattctt cgctgcttaa gaattatctt
    21541 gatggtaaat ctagttgtgc catgcatttt ctcatgatga atctaaggtg ttaattcatt
    21601 gatgtgtgct gtttcttgtt gcttcattat cttgatggtg aatttcgttg tctaatgcac
    21661 tttttcaaat gattattcta attgttacag caataactag atggattttc ttagatgttc
    21721 acttttctag atgtttatac ttatcttgat gttttctaaa cagaactttt aaacgcttta
    21781 ttttttagta gattgtttaa aatttcttag ttgatggtta ttgttgttga tttgaataat
    21841 ttgtagcgaa tattttgtaa cagggctact aagaattttt ataacaagaa gtgatttcaa
    21901 tcgtactaat tttcagcgtt tgatgacaaa agtgtatgtc caaacttaaa taatggcgat
    21961 aaaatttaag tcatgggtac atttaatgtt ttttgattca aacttccatt ttggtcaatt
    22021 tgcttttttt ataacatagt tgaatatcaa taaaagatta gcaagttagt tcaaaatggc
    22081 tatgtattgt tttttgaaat acataggttt gaaatctata ttaattatgc accaaataat
    22141 ttaattagac tctatcgaaa atttccaaat tttgcttact atctttctaa ttttactttt
    22201 atattgttcg caatgcataa aaacaagcgt tttcctagga gttttcatta atttctttta
//
LOCUS       DANJFZ010000035        22029 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.035, whole genome shotgun sequence.
ACCESSION   DANJFZ010000035 DANJFZ010000000
VERSION     DANJFZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.035"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            59..1651
                     /locus_tag="PWN45_001163"
     CDS             59..1651
                     /locus_tag="PWN45_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL0622941.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(1980..3506)
                     /gene="spa"
                     /locus_tag="PWN45_001164"
     CDS             complement(1980..3506)
                     /gene="spa"
                     /locus_tag="PWN45_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDL0622942.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(3927..4679)
                     /gene="sarS"
                     /locus_tag="PWN45_001165"
     CDS             complement(3927..4679)
                     /gene="sarS"
                     /locus_tag="PWN45_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDL0622943.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            complement(5048..6046)
                     /gene="sirC"
                     /locus_tag="PWN45_001166"
     CDS             complement(5048..6046)
                     /gene="sirC"
                     /locus_tag="PWN45_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDL0622944.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(6043..7038)
                     /gene="sirB"
                     /locus_tag="PWN45_001167"
     CDS             complement(6043..7038)
                     /gene="sirB"
                     /locus_tag="PWN45_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDL0622945.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(7054..8046)
                     /gene="sirA"
                     /locus_tag="PWN45_001168"
     CDS             complement(7054..8046)
                     /gene="sirA"
                     /locus_tag="PWN45_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDL0622946.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            8277..9257
                     /gene="sbnA"
                     /locus_tag="PWN45_001169"
     CDS             8277..9257
                     /gene="sbnA"
                     /locus_tag="PWN45_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDL0622947.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            9254..10264
                     /gene="sbnB"
                     /locus_tag="PWN45_001170"
     CDS             9254..10264
                     /gene="sbnB"
                     /locus_tag="PWN45_001170"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDL0622948.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            10285..12039
                     /gene="sbnC"
                     /locus_tag="PWN45_001171"
     CDS             10285..12039
                     /gene="sbnC"
                     /locus_tag="PWN45_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDL0622949.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            12032..13288
                     /gene="sbnD"
                     /locus_tag="PWN45_001172"
     CDS             12032..13288
                     /gene="sbnD"
                     /locus_tag="PWN45_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDL0622950.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            13278..15014
                     /gene="sbnE"
                     /locus_tag="PWN45_001173"
     CDS             13278..15014
                     /gene="sbnE"
                     /locus_tag="PWN45_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDL0622951.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            14923..16773
                     /gene="sbnF"
                     /locus_tag="PWN45_001174"
     CDS             14923..16773
                     /gene="sbnF"
                     /locus_tag="PWN45_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDL0622952.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            16748..17524
                     /gene="sbnG"
                     /locus_tag="PWN45_001175"
     CDS             16748..17524
                     /gene="sbnG"
                     /locus_tag="PWN45_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDL0622953.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            17524..18726
                     /gene="sbnH"
                     /locus_tag="PWN45_001176"
     CDS             17524..18726
                     /gene="sbnH"
                     /locus_tag="PWN45_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDL0622954.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            18730..19494
                     /gene="sbnI"
                     /locus_tag="PWN45_001177"
     CDS             18730..19494
                     /gene="sbnI"
                     /locus_tag="PWN45_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDL0622955.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            19690..20097
                     /locus_tag="PWN45_001178"
     CDS             19690..20097
                     /locus_tag="PWN45_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622956.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            20147..20773
                     /locus_tag="PWN45_001179"
     CDS             20147..20773
                     /locus_tag="PWN45_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0622957.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            20985..21761
                     /locus_tag="PWN45_001180"
     CDS             20985..21761
                     /locus_tag="PWN45_001180"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDL0622958.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            21905..22027
                     /locus_tag="PWN45_001181"
     CDS             21905..22027
                     /locus_tag="PWN45_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622959.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
BASE COUNT         7195 a         3470 c         4284 g         7080 t
ORIGIN      
        1 aaataggttg tagattaatc agaatgataa atattttata taaagagagg gagtcattat
       61 gacactactt actgtaaatc cattcgataa tgtcggatta tcagccttag ttgcagcagt
      121 acctattatt ttatttttat tatgcttaac cgtttttaaa atgaaaggca tttatgcagc
      181 attgacaact ttggttgtta cattgattgt ggctttattt gtatttgaat taccagcgcg
      241 tgtatcagca ggtgcgatta cagaaggcgt tgttgccggt attttcccaa taggatatat
      301 cgttttaatg gcagtttggt tatataaagt ttctattaaa acaggacaat tttctattat
      361 tcaagatagt attgcaagta tttcagtgga ccaaagaatc caactattat taattggatt
      421 ttgtttcaac gcatttttag aaggtgcagc aggatttggt gtgccaattg cgatttgtgc
      481 agtattatta attcaacttg gatttgaacc attaaaagca gcgatgttat gtttaattgc
      541 taatggtgcg gcgggtgcct ttggtgcaat tggtttacca gttagtatta ttgatacgtt
      601 taacttaagt ggaggcgtta caacattaga tgttgcgaga tactcagcat taacacttcc
      661 aattttaaac tttattattc catttgtttt agtattcatt gtagatggta tgaaaggtat
      721 taaagaaatt ttacctgtca ttttaacagt gagtggtaca tatactggat tacaattatt
      781 attaacaata ttccatggtc cagaactagc agacattatt ccatcactag caacaatggt
      841 ggtgttagca tttgtttgtc gtaaatttaa accgaaaaac attttcagat tggaagcgtc
      901 tgaacataaa attcaaaaac gaacgcctaa agaaattgtc tttgcttgga gtccgttcgt
      961 aattttaact gcctttgtat tagtatggag tgcaccattc ttcaaaaaat tattccaacc
     1021 tggaggtgca cttgaaagtt tagtaataaa attgccaatt ccaaatactg tgagtgattt
     1081 atcgcctaaa ggaattgcgt tgcgtctcga tttaattggt gcaactggga cagcgatttt
     1141 attaacagta attattacaa ttttaattac gaagttaaaa tggaaaagtg caggtgcttt
     1201 attggtcgaa gcaattaaag aattatggtt accgatcctt acaatttcag ctatcctagc
     1261 tattgctaaa gttatgacat acggtggttt gactgtagca attggacaag gtattgctaa
     1321 agcgggagca attttcccat tattctctcc agtattaggt tggattggtg tgtttatgac
     1381 tggttcagtt gtaaataaca atactttatt cgcacctatt caagcgacag tggcacaaca
     1441 aatttcaaca agcggttcat tacttgtggc agctaacact gcaggtggtg tagcagcgaa
     1501 acttatttca ccacaatcaa ttgccattgc gactgcagct gttaaaaaag ttggtgaaga
     1561 atctgcatta ttaaaaatga cgttaaaata cagtattata tttgttgctt ttatttgtgt
     1621 ttggacgttt atactaacgt taatattcta aatataaata atgttgtcac ttggattcaa
     1681 atgacatttt aaatctaatt attcatgaat cgaactagta cgaaatgcaa tgagcatctt
     1741 gtctagttcg attttttaat gcctaaaaat gtcatatatg taatcagagt agaaagtgtt
     1801 gaggcgtttc agaagttgtt tagaaaagta agtaaaataa aaaatgcact gagcaacaaa
     1861 agatgttgct cgtgcattta gatgattctt atcatttcaa ataagaatgt gttaatcaac
     1921 gtatataagt taaaattggt ttggataaaa tgatatctat cgttgtgtat tgtttgtttt
     1981 tatagttcgc gacgacgtcc agctaataac gctgcaccta aggctaatga taatccacca
     2041 aatacagttg taccgatgaa tggattttct tcaccagttt ctggtaatgc ttgagctttg
     2101 ttagcatctg catggtttgc tggttgcttc ttatcaacaa caagttcttg accaggtttg
     2161 atcatgtttt tatcagctaa tttgttatct gcagcaattt tgtcagcagt agtgccgttt
     2221 gcttttgcaa tgtcatttac tgtatcacca ggtttaacga catgtactcc gttaccatct
     2281 tctttaccag gtttgttgcc atcttctttg ccaggcttgt tgccgtcttc tttaccaggt
     2341 tttttgttgt cttctttacc aggcttgttg ccatcttctt taccaggttt tttgttgtct
     2401 tctttaccag gcttgttgcc gtcttctttg ccaggcttgt tgttgtcttc tttaccaggc
     2461 ttgttgttgt cttctttgcc aggcttgtta ttgtcttctt tgccaggctt gttattgtct
     2521 tcctcttttg gtgcttgagc atcgtttagc tttttagctt ctgctaaaat ttctttgctc
     2581 actgaaggat cgtctttaag gctttggatg aagccgttac gttgttcttc agttaagtta
     2641 ggtaaatgta aaatttcata gaaagcattt tgttgttctt tgttgaattt gttgtcagct
     2701 tttggtgctt gagcatcatt tagcttttta gcttctgcta aaaggttagc gctttggctt
     2761 gggtcatctt ttaggctttg gatgaaacca ttgcgttgtt cttcgtttaa gttaggtaaa
     2821 tgtaagattt catagaaagc attttgttgt tctttgttga atttgttatc cgctttcggt
     2881 gcttgagatt catttaactt tttagcttct gacaataggt tagcactttg gcttgggtca
     2941 tcttttaagc tttggatgaa accattgcgt tgttcttcgt ttaagttagg catattcaag
     3001 atttcataga aagcattttg ttgttctttg ttgaaattgt tatcagcttt cggtgcttga
     3061 gattcgttta attttttagc ttcacctaaa acgttagtgc tttggcttgg gtcgtcttta
     3121 agactttgaa tgaagccgtt acgttgcgct tcgtttaagt taggcatgtt caagatttca
     3181 tagaaggcgc tttgttgatc tttgttgaag ttattttgtt gcgcatcagc ttttggagct
     3241 tgagagtcat taagtttttg agcttcacct aaaacgttag cactttggct tggatcatct
     3301 ttaaggcttt ggataaaacc attgcgttga tcagcattta agttaggcat atttaagact
     3361 tgataaaaag cattttgttg agcttcatcg tgttgcgcag catttgcagc aggtgttacg
     3421 ccaccagata taagtaatgt acctaaagtt acagatgcaa tacctacacc tagtttacga
     3481 attgaataaa tgtttttctt tttcaaatta ataccccctg tatgtatttg taaagtcatc
     3541 ataatataac gaattatgta ttgcaatact aaaatctata tttataatta aatttaaagg
     3601 taagttttac aacttataaa ataaatattt tgctgaaaga tttattcagg aagtaaaagg
     3661 cttaaaaccg caaaatcacg ctatttcgat taataaaaac agataaaata tagagatatt
     3721 ttttataatg aaagcggttt aatacatatg agttaaaaaa tatttttaag aataaagtgg
     3781 ggctttgaat gtgctgaggt ttaatttcgg aaatggtttt aagtgatata gaaaatagaa
     3841 agttgttcat tatgtgctga ggttttatta aaaaatagaa aacacaagtg cactttagaa
     3901 tacagcacac ttgcgtaata gagatattat tcaaaaacaa gatgtaaatg atctttatct
     3961 gctaataatt gattcacttg agctaataat tgttcagcat ggtcttgctg cgcgtcatcc
     4021 atatgaatta aaatttttct ttcatcttca gttgagcgtt cttttattag atagccttgc
     4081 ttttttaaat tattgagagc tctaacagtt tgagggtatt tatggtggat tgtttcaatt
     4141 aaatctttaa gaagaacgat gtttttattt tgagaagtga taatagctag aattgtgaat
     4201 tctacaaaac ttaatgttag atgttttttg ataatattct tgaaatacat tgtatacatc
     4261 atcaagttta gaaattcttt actatctttt ggtatcatct gtgattcact ttgatctgca
     4321 aggttaaatt gtttaatgat ttgatcaaac aatgtaacac gttctgcaat tttctctcgt
     4381 tgttcttcag atattgaaat gtaagtatta cgctcatcaa ttttacttcg aactttacta
     4441 atatatgaat gtttcacaag tacttttata tgctgtacta aatccgattg tttataacat
     4501 aaatctgaaa caatcttctt aaatggaagt gtgttttctt gttgatgaaa taaataagtc
     4561 agtaatataa attcttttat agtcatatcg acttcaggct tgactttttt cttaaaacga
     4621 aacatatatg cttcaatgat tataaaatct ctaattttgt catggttatt atatttcatt
     4681 gttttatctc cttgtatatg cactttattt taatataaca caatataata agaaaaataa
     4741 tccataattt acaattaaaa ataatatgat atttttatta aaatttgtgt ttttgaataa
     4801 atgacattat caaacactat atttaaataa gaaatagtta tcgatatttg aaaaatcgaa
     4861 gaatattgat tgatattata gaatatgagt cggtgttatt tgtatataaa gtattaatat
     4921 gtaattaaga aagtcgacga ttgttaacaa taacaacaac tatatgtata aaaaaacctc
     4981 gcaacggtta gttaactgaa tcatttagct aacttcgttg tgaggtcatt ttgttttaat
     5041 aatatcgtta taactttttc acggttaata ataagtatat gaagaatggg gcaccaaaag
     5101 cagcaataaa tacacctgct ggcacttctt taggcaagaa taaggtacgc ccaattaagt
     5161 ctgcaataac aattgatatg gcaccaatca ttgctgacat tagtaacttt ttagcataac
     5221 ttccgcgaac gattgttttc gcgatatgtg gtgcgattaa accgacaaac ccaatgttac
     5281 ctactaaact gattgccata gatacgagta tagtagaagt gattaattgg attagtttca
     5341 tacgttgtac atgtaagcct aagccaatcg ctacagggtc atcaagtata gatattttca
     5401 tttttggtat aacaagaaat aacaacggca caacagctaa aataaccata cccaaaatga
     5461 ttgtatcttt aaacgtagca ccgtaaagac ttccgactag ccatgtataa gctttggcag
     5521 cagataattg cttcgttgta atgagtaatc cttggacaag cgcaataaac aacgtttgca
     5581 tcgaaatacc gatgattatg agtgttgtcg ggcgtatttg tcctttcgtt tgaaacacta
     5641 atagtatcat cattgcaact gcgccaccta atactgcaaa tagtggaagt aaatgtattg
     5701 ttaaatggct gaaaaatgca ataaagacaa cagcacttaa gctagcacca cctgtgatac
     5761 cgataatatc aggtgaggca attggatttt ttaatacatt ttgcaacatt aaaccactca
     5821 ttcctagtgc ggcacctgct aaaatcgcaa gtgtaatgcg aggtaagcgt aatacttcta
     5881 aagtgaattg atccatactg tcatttggat ttataaagta catcagtacg cgttgtaatg
     5941 gtataaagct tgaaccaatc atcatactta ccactgaaac gatggctaaa aagattaacg
     6001 cgaagatgag atggtaattg tcttttttat taatcttttc ggtcataagc gttgacgtcc
     6061 tttcttcata atatagatta agacaatagc gccaatgaca gcggtaacga caccgatagg
     6121 caactctagt ggcttaatta ttatacgagc aacaatgtct gaaatgatca ttaggattgc
     6181 tccagctaat gcagtaaaag gaattaaata cttatagttt ggtggtaata atcgtttgct
     6241 aatattcggt acgataagac ccacaaagac gattgatcca gctacggcta ccgaaatacc
     6301 ggctaacata ctgatgagca taataatcat ccatttgatt aattttatgt tttgaccgag
     6361 gccggttgca atgtcgtcac ttgtcatcaa gatgttgatg tgtgcagcca tgctaaatgc
     6421 aattaaaata agtatcaata caagcggaat aatccatggg atatcccaaa tattacgtaa
     6481 tgaaacggag ccacttaacc aaaataatag gccttgtaag tctgtttcgt tcataataag
     6541 tatgccttga gtaaaggctg taaatagcat cgcaatcgca gcacctgcca aaatgacacg
     6601 gtgaggtgag aatagtgttt gtctaaacat acctagtgca acaactaata cagtaacaac
     6661 aatagccccc aaaaatgcaa taactacaat cattttaaaa gattgaattt ggataaatgt
     6721 aatactaaaa atgacaaaaa atactgcgcc tgcattgaca ccgaaaagcc ctggtgaggc
     6781 tattgggttt cgtgtaagtg cttgcatcaa caaacctgag acagcaaggg cagcaccagt
     6841 caataacgca atgattgttc tcgacgcccg tgcaccagtg acaacatcat gtaaatcgtt
     6901 ttcactatca aagttgaata acgcctgtat caccgtacct ggtgacacaa gcgtatttcc
     6961 aatcattaaa cttaagatag ctactattgc aagacataaa ccagcaataa cgatttggta
     7021 ttttggttta agtagcatcg taaaactcct taattatttt gattgttttt caatatttaa
     7081 cttttcatat aaatcgtcaa taagttttaa tgaagattta tatccgccag ctaagttcca
     7141 agtgatttca tctaaatcat cagatacttg gttgttttta actgcgtcta aatttttcca
     7201 ctctttactt gaagtccatt cgctttcagt ctttttaact aatgcagcat ctttcgcatt
     7261 tggatctgat tttactacaa aaatatgatc agcgttcatt aatggaatgc tttctttaga
     7321 tgtaagttgg ataatatctt taccattatc aacttgtttt tgtaagtctt tattacgttt
     7381 gaatcctaaa tcatttaaga tttcaccagc atatccacca gcataaattc ttgtatgatc
     7441 agcacggaag ttaacaactg aagctttcaa tggccatgca tctttatact ttgcttttgc
     7501 atctttttgg aatgcagcta ctttatcatc gtacttttta agtaaatctt cagcttcttt
     7561 ttctttccct aaagctttcc ccattaactt agttgtatct ttgaatttga aaactgtatc
     7621 agtagaaact gttggtgcga ttttagataa ttgatcgtaa actttttcat ttctaacttt
     7681 tgacgcgaca attaagtccg gttttaattt agagatttcc tctaagttag gtgcaggttc
     7741 ttgacctaca atcttagtat cttttaaatc attttttatg tattcgaatt tcggtttttg
     7801 tgtccatgat tctacagcac ctacaggttt aacacctaaa gatacagcga cgtcagtggc
     7861 accttgatat agcgtaacaa cacgctttgg tttcccttta atttcagttg tacccattgc
     7921 atgtttaatt gaagttgttt ccttatcttt gttatcagat gattgtttat ttgaattccc
     7981 actacatcct gctaaaacaa gtaggaaagc aagcgtaaca acaagcattt taattacttt
     8041 attcattgac taattagcct ccttcgtgat gtatgacaat gagaatcatt atcacgattt
     8101 agtatgaatt aaattttttc ctaagtcaat aaaatattta tgatttacat gcaacttata
     8161 attatttgac atataaatgc ataaaaaata taatcctaat tacttgatag tgagaatcat
     8221 tatcaattag gtaacacaca atattataga attttaaatt tgaggaggaa gcgcttttga
     8281 ttgaaaaaag tcaagcatgt cacgattcat tgttagattc tgtagggcaa acacctatgg
     8341 ttcaacttca tcaactattt ccgaaacatg aagtgtttgc aaagttagag tatatgaatc
     8401 ctggaggcag catgaaagat cgacctgcca agtacatcat tgaacatggt attaaacatg
     8461 gtttaatcac tgagaataca catttaattg aaagtacttc tggtaattta ggcattgcgt
     8521 tggcaatgat agctaaaatc aagggattaa aactcacgtg tgttgttgat cctaaaatat
     8581 caccaacaaa tttgaaaatt attaaaagtt atggtgccaa tgtagaaatg gttgaagaac
     8641 ctgatgcaca tgggggttat ttaatgactc gtattgcaaa ggtgcaagaa ctgttagcca
     8701 ctattgacga tgcatattgg attaatcaat atgcgaatga gttaaattgg caatcccatt
     8761 atcatggtgc aggcacagag attgttgaaa caattaagca acctatagat tattttgtcg
     8821 cgccagtcag cacgacaggt agcattatgg gtatgagtag aaaaataaaa gaagtgcatc
     8881 caaacgcaca aattgttgct gttgatgcga aagggtcagt catttttggt gacaaaccta
     8941 ttaatagaga attacctggt atcggtgcta gtcgtgtacc cgaaatattg aatagatcag
     9001 aaattaatca agtgatccat gtagatgatt atcaatctgc tttgggctgt cgaaaactga
     9061 ttgattatga aggcatattt gccggaggtt caacaggttc gattattgca gcgattgagc
     9121 agttgataac gtcaattgaa gaaggtgcaa caattgtcac gattttacca gatcgaggcg
     9181 atcgttactt agatttagtt tattcagata catggttaga aaaaatgaaa tcaagacaag
     9241 gagttaaatc agaatgaata gagagatgtt gtatttaaat agatcagata ttgaacaagc
     9301 gggaggtaat cattcacaag tttatgtgga cgcattaaca gaagcattaa cagcccatgc
     9361 gcacaatgat tttgtacaac cgcttaagcc gtatttaaga caggatcctg aaaatggaca
     9421 catcgcagat cgaattattg caatgccaag tcatatcggt ggtgaacacg caatttcagg
     9481 tattaagtgg ataggtagta agcacgacaa tccatcgaaa cgtaatatgg agcgtgcaag
     9541 tggcgtcatt attttgaatg atccagaaac gaattatcca attgcagtta tggaagcaag
     9601 tttaattagt agtatgcgta ctgcagcagt ttcagtgatt gcagcaaagc atttggctaa
     9661 aaaaggattt aaagacttaa caatcattgg atgcgggcta atcggagaca agcaattaca
     9721 aagtatgtta gagcaattcg atcatattga acgcgtgttt gtttacgatc aattctctga
     9781 agcatgtgca cgctttgttg atagatggca acaacagcgt ccggaaatta attttattgc
     9841 gacagaaaat gctaaagaag cagtatcaaa tggtgaagta gtcattacat gtaccgtaac
     9901 ggatcaacca tacattgaat atgattggtt acaaaagggt gcatttatta gcaacatttc
     9961 tatcatggat gtgcataaag aagtctttat taaagctgac aaagtcgtag tagatgactg
    10021 gtcacaatgt aatcgagaaa agaaaactat taaccaattg gtgttagaag gtaaattcag
    10081 caaagaagct cttcatgctg aactaggaca acttgtgaca ggtgacatac caggacgtga
    10141 agacgatgat gagatcatat tacttaatcc gatgggtatg gctatcgaag atatttcaag
    10201 tgcttatttt atttatcaac aggcacaaca acaaaatatt gggacaacat tgaacctata
    10261 ttaagaatgc gaggtgtctg aacattgcag aatcatacag cagtcaatac agcacaagcg
    10321 ataatattaa gagatttagt tgatgcatta ttatttgaag atatagccgg aattgtatcg
    10381 aatagtgaga ttactaaaga aaacggacaa acgcttttga tatacgaacg tgaaacacaa
    10441 caaataaaga tacctgttta ttttagtgct ttaaatatgt ttcgttacga aagttcacaa
    10501 ccaattacga tagagggaag ggtgtctaag caacctttaa cggcagctga attttggcaa
    10561 acaattgcta atatgaattg tgatctaagt catgaatggg aagtggctcg cgttgaagaa
    10621 ggactgacta ctgctgccac acagcttgct aaacaattat cagaattaga tttagcgtca
    10681 catccttttg tgatgtcaga gcagtttgca agtttaaaag atcgtccatt tcatccatta
    10741 gctaaagaaa aaagaggatt aagagaagcg gattatcaag tgtatcaagc tgaattaaat
    10801 caatcatttc ctttaatggt tgcagcagtt aaaaagacac atatgattca tggcgatact
    10861 gcaaatatcg atgaattaga aaatttgaca gtacctataa aagaacaagc gacagacatg
    10921 ttaaatgatc aagggttatc aatagatgac tatgtactat ttccggtaca tccttggcaa
    10981 tatcagcata ttctgccgaa cgtctttgcg aaagagatta gtgaaaagtt ggttgtacta
    11041 ttaccgttaa aatttggaga ttatctgtcg tcttcaagta tgcgttcatt aattgatatt
    11101 ggcgcaccgt ataaccatgt caaagtacca tttgcaatgc agtcattagg cgcattaagg
    11161 ctaacgccta cgcgttacat gaaaaacgga gaacaagcag aacaattatt acgtcagctt
    11221 atagaaaaag atgaagcact agctaagtat gtcatggttt gtgatgaaac agcttggtgg
    11281 tcatatatgg gtcaagataa tgatattttc aaagatcaat taggtcatct aactgttcag
    11341 ctaagaaagt atcccgaagt gctagccaaa aatgatacgc aacagctagt gtcaatggca
    11401 gcactcgcgg caaatgatcg cactttatat caaatgattt gtggaaaaga taatatttct
    11461 aaaaatgatg tcatgacgtt atttgaagat atcgcgcaag tctttttaaa ggtaacacta
    11521 tcatttatgc aatacggcgc attaccagag ttgcatggtc aaaatatatt gttgtcattt
    11581 gaagatggac gtgtacaaaa atgcgtgtta cgtgatcatg atactgtcag aatttataaa
    11641 ccatggctaa cagcacatca gctttcattg ccgaagtatg tcgtcagaga agatacacct
    11701 aatacgctaa ttaatgagga tttggaaaca ttctttgctt attttcaaac attagctgta
    11761 tcggtaaatc tatatgccat tattgatgca attcaagatt tatttggtgt aagtgagcat
    11821 gaacttatgt cgttgttaaa acaaatttta aaaaatgaag tggcaactat ttcctgggtt
    11881 acaactgatc agctagctgt cagacacatt ttatttgata aacagacgtg gccattcaaa
    11941 caaattttat taccattgct atatcaacgt gatagtggtg gaggtagtat gccttcaggt
    12001 ttaactaccg taccaaatcc aatggtgaca tatgattaat cagtctatat ggcgcagtaa
    12061 ctttcgcatt ttatggctca gtcagtttat agcgattgct ggactgacag tacttgtgcc
    12121 attattgcca atttatatgg catcactaca aaatctatca gtcgtagaaa tacagttgtg
    12181 gagtggtata gcgattgctg ctccagctgt aacgacgatg atagcttcgc cgatatgggg
    12241 gaagctaggt gataagatca gccgaaaatg gatggtgtta agagcgttac ttggtttggc
    12301 ggtatgctta tttttaatgg cattgtgtac gacaccatta cagtttgtac ttgtgaggtt
    12361 attgcaggga ctatttggtg gtgttgttga tgcatcaagt gcgtttgcga gtgcagaggc
    12421 gccagctgaa gatcgtggaa aggtattagg aagactgcaa agttcagtca gcgcagggtc
    12481 tcttgtgggg ccattaattg gcggtgttac agcttcgata ttaggtttta gtgcgttact
    12541 gatgagtatt gccgttatta cttttattgt ctgtattttc ggtgcattaa aattgattga
    12601 aacgacacat atgccaaaat cacaaacacc aaatattaat aaaggtattc gccgttcatt
    12661 tcaatgtcta ttatgcacac aacaaacatg tcgatttatt atcgttggcg ttttagcaaa
    12721 ctttgctatg tatggcatgc taactgcatt atcaccactt gcttcatcag tgaatcatac
    12781 agcgatagat gaccgtagtg tgattggatt tttacagtcc gcattttgga cggcttcgat
    12841 attaagcgcg cctttatggg gacgctttaa tgataaatca tatgttaaat cagtatatat
    12901 atttgccacg attgcatgtg gttgtagtgc gatactgcaa ggtttagcga cgaatataga
    12961 gtttttaatg gctgcaagaa tacttcaagg attaacatat agtgcattga ttcaaagtgt
    13021 catgtttgtt gtcgtgaatg cgtgtcatca acaacttaaa ggcacatttg ttggaacgac
    13081 gaacagtatg ttagttgttg gtcaaattat tggcagtctt agtggcgctg ccattacaag
    13141 ttatactaca ccagctacta cgtttatcgt tatgggcgta gtatttgcag taagtagttt
    13201 atttttaatt tgttcaacca tcactaatca aatcaacgat cacacattaa tgaaattatg
    13261 ggagttgaaa caaaaaagtg caaaataaag aattaataca acatgcagcg tatgcggcta
    13321 tcgaacgcat tttaaatgaa tattttagag aagaaaattt atatcaagta ccacctcaaa
    13381 atcatcaatg gtctatacaa ttatcagagc tcgaaacttt aacgggtgaa tttcgctatt
    13441 ggtctgcgat ggggcatcat atgtatcatc cagaggtatg gcttatcgat ggaaaaagta
    13501 aaaaaataac aacttataaa gaagcaattg cgcgtatttt gcaacatatg gctcaaagtg
    13561 cagataatca aacggcagtg caacaacata tggcgcaaat tatgtctgac atcgataata
    13621 gcattcatcg cacggcacgt tatttgcaaa gtaacacaat agactacgta gaggatcgtt
    13681 atatcgtttc agaacaatct ttatacttag gtcatccatt tcatccgact cctaagagtg
    13741 caagtgggtt ttcagaagca gatttagaga aatatgcacc cgaatgtcat acatcattcc
    13801 aattgcatta tttagctgtg catcaagatg ttctgctcac gcgctatgta gaaggtaaag
    13861 aagatcaggt tgagaaagtg ttgtatcaat tagcagacat agatatatca gagataccca
    13921 aagattttat tttattacca acacatcctt atcaaatcaa tgtgttgcga cagcatccac
    13981 agtatatgca atatagtgaa caaggtttaa taaaagacct tggcgtttcc ggtgattcag
    14041 tgtacccgac gtcttcggtt agaactgtat tttcaaaagc attaaacatt tatttaaaat
    14101 taccgataca cgttaaaatc actaatttta tacgtacgaa tgaccttgaa cagattgaac
    14161 ggacaattga tgccgcgcaa gttatcgcat cagtcaaaga tgaggttgaa acaccccatt
    14221 ttaaattgat gtttgaagaa ggatatcgtg cattgttacc gaatccatta gggcaaacag
    14281 ttgaacctga aatggattta ttaacaaata gtgccatgat tgttcgtgaa gggataccga
    14341 attaccatgc tgataaagat attcatgtat tggcgtcatt atttgaaacg atgcctgatt
    14401 caccgatgtc taagttatca caagtgattg agcaaagtgg tttagcacca gaagcatggc
    14461 ttgaatgtta tttgaatcgt acattattgc cgatattaaa gctgtttagt aacacaggca
    14521 ttagtctaga agcacatgta caaaatacat taattgaatt aaaagatggc atacccgacg
    14581 tatgctttgt cagagatctt gaaggcattt gtctatctag aacgattgct actgaaaaac
    14641 agcttgtgcc aaatgttgtg gcagcatcaa gccctgttgt atatgcacat gatgaagcat
    14701 ggcatcgtct taaatattac gttgtagtaa atcacttagg acatttagta tcaactattg
    14761 gtaaagcgac tagaaatgaa gttgtgttat ggcaacttgt agcgcatcgt cttatgactt
    14821 ggaaaaaaga atacgcgaat aacgcagtat ttgttgactg tgtagaagat ttatatcaaa
    14881 cgccgaccat tgcggctaaa gcgaatttga tgagtaaatt gaatgattgt ggtgcaaacc
    14941 ctatttatac acatatacca aatccaattt gtcataacaa ggaggtatcg tattgtgaat
    15001 caaacaattc ttaatcgtgt aaagactaga gtgatgcacc aactggtatc atcacttatt
    15061 tatgagaata ttgttgtgta taaagcgtca tatcaagacg gtgtcggtca ttttacaata
    15121 gaaggacatg attcagagta tcgttttact gctgaaaaga cacatagctt tgatcgtata
    15181 cgtatcacat caccaattga gcgtgtcgta ggagatgagg cagatacaac aacagactat
    15241 acacaattat tgagagaggt tgtatttaca tttcctaaaa atgatgaaaa gctagaacaa
    15301 tttattgtcg agttattaca gacagaatta aaagatacgc aaagtatgca gtatcgagaa
    15361 tcaaacccac cagcaacacc tgagacattt aacgactatg aattttatgc gatggaaggg
    15421 catcagtatc atccaagtta caaatcacgt ttaggattta cgttgagtga taatttgaaa
    15481 tttggtcctg attttgtacc aaacgttaaa ctgcagtggt tagctatcga caaagataaa
    15541 gtagaaacga cggtatcaag aaatgttgta gttaacgaaa tgttacgtca acaagttggc
    15601 gataagactt atgaacattt tgtacagcag attgaagcat ctggcaaaca tgtaaatgat
    15661 gttgagatga tacctgtaca cccatggcag tttgaacatg tcatccaagt tgatttggct
    15721 gaagaaaggc ttaatggcac agtactatgg ttaggggaaa gtgatgagct atatcaccct
    15781 caacaatcga ttcgtacgat gtcgccaata gacacgacaa aatattattt aaaggtacca
    15841 ataagtataa cgaacacttc aacgaaacga gtgttggcgc ctcatacaat tgaaaatgca
    15901 gcgcaaatta cggattggtt aaagcagata cagcaacaag atatgtattt aaaagatgaa
    15961 ttaaagacag tttttctagg ggaagtctta ggacagtctt atttaaatac acaactttcg
    16021 ccttataaac aaactcaagt ttatggtgcg ttaggtgtta tatggcgtga aaatatatat
    16081 catatgttaa tcgatgaaga ggatgcgata ccatttaatg cactttatgc aagtgataag
    16141 gatggtgtac cattcattga aaattggatt aaacaatatg gttctgaagc ttggacaaag
    16201 caatttttag ctgtagcgat tcgtccaatg attcatatgc tttattatca cggtattgcc
    16261 tttgaatcgc atgcacaaaa tatgatgctc attcatgaaa atggttggcc tacacgtatt
    16321 gccttaaaag atttccatga tggtgttcgt tttaagcgtg agcatttaag tgaagctgct
    16381 tcacacctga cattaaagcc aatgccagaa gcacataaaa aagtgaatag taattcattt
    16441 attgaaacag atgacgaacg tttagtacgc gactttttac atgatgcatt tttctttatt
    16501 aatatcgccg aaatcatctt atttattgaa aagcaatatg gtatcgatga ggagctgcaa
    16561 tggcaatggg ttaaaggcat catcgaggcg tatcaagaag catttccaga gttgaataac
    16621 tatcaacatt tcgatttgtt tgaacctacg attcaagttg aaaagttaac gacacgtcga
    16681 ttattaagtg actccgagtt aagaattcat catgttacaa atccattagg tgtaggaggt
    16741 atcaatgatg caacaactat ctctgaaaca tagattaaac aatggtgatt cagtttatgg
    16801 catttttaat tctataccgg acccattgat gatcgaggtt atcgcagcaa gcgggtatga
    16861 ctttgttgtg attgatacag aacacgtggc gattaatgat gagacactag cgcatttaat
    16921 tcgtgcagct gaagcagcgc atattatacc aattgtacgt gtcactgcag tgatagatag
    16981 agatatcatt aaagtgttgg atatgggtgc gagaggtatt attgtgccac acgttaaaga
    17041 tcgtgagaca gttgagcata ttgtgaaatt aagtcgttat tacccgcaag gattaagaag
    17101 tttgaatggt ggtcgcatgg caagatttgg acgtacacca ttacttgatg caatggagat
    17161 ggctaatgag catattatgg tgattgccat gatagaagat gttgaagggg ttatggccat
    17221 tgacgatata gcacaagtcg aaggtttaga catgatagtc gaaggtgccg cagatttatc
    17281 gcagtcactt ggcataccat ggcaaacgcg tgatgatcaa gtaacatcac atgttcaaca
    17341 tatttttgaa gttgtgaatg cacatggtaa acatttttgt gcattaccac gtgaagatga
    17401 agatattgca aaatggcagg cacaaggtgt acaaacattt attttaggtg atgatcgcgg
    17461 aaaaatatat cgccatttaa gtgcatctct agcgacgtct aaacagaaag gggatgaagg
    17521 ctaatgcgta tagttcaacc tgttattgaa caattaaaag cacaatctca tccagtttgt
    17581 cattatatct atgatttagt cggactggaa catcatttgc aacatattac atcgtcattg
    17641 ccgagtaatt gtcaaatgta ctatgcaatg aaagcaaata gtgaacgaaa aatcctagat
    17701 acaattagtc agtatgttga aggattcgaa gttgcatctc aaggtgaaat agcaaaaggt
    17761 cttgctttta aaccagcaaa tcatattatt tttggtggcc ctggtaagac agacgaggaa
    17821 ctaagatatg cagtaagtga aggtgttcag cgtattcatg ttgaaagtat gcatgaatta
    17881 caacggctaa atgccatctt agaagatgaa gataagacac aacacatttt attgcgtgtt
    17941 aatttagcag gaccatttcc caatgcaacg ttgcatatgg caggacgccc aacacaattt
    18001 ggtatttctg aagacgaagt tgatgatgtc attgaagctg cgctcgcaat gccaaagatt
    18061 catctagatg gatttcattt tcattctatt tctaacaatt tagactcgaa tttacatgtc
    18121 gatgtagtga aactttattt taaaaaagca aaggcatggt ctgaaaaaca tcgatttcca
    18181 ctcaaacata tcaatcttgg tggtggcata ggcgttaact atgcagattt aactaaccaa
    18241 tttgaatggg ataattttgt agaacgtttt aaaacactta tcgttgagca agaaatggaa
    18301 gatgtgacat tgaactttga atgtgggcgc tttattgtgg cacatattgg ttactatgtg
    18361 acagaagtgc tagacattaa gaaagtacat ggtgcttggt atgccatatt aagaggaggt
    18421 acgcaacaat ttagactgcc ggtatcttgg caacataacc atccttttga catttatcgc
    18481 tataaggaca atccatattc atttgaaaaa gtttcaattt cgagacagga cacaacgtta
    18541 gtcggtcaat tatgtacacc gaaagatgtc tttgctagag aagtacagat agacgcaatc
    18601 agtacaggcg acgttattgt tttcaaatat gcaggtgcat acggatggtc tatttcacat
    18661 cacgatttct taagccatcc acatcctgaa tttatttatt taacgcaaac aaaggaggat
    18721 gaataactat tgaatcatat tcatgaacat ttaaaattgg taccagtaga taagattgat
    18781 cttcacgaaa cattcgaacc tttaagattg gaaaaaacga aaagtagtat tgaagcagat
    18841 gattttatac gtcatcctat tttagtgaca gcgatgcaac atggtagata tatggttata
    18901 gatggtgtgc atcggtatac aagtttgaaa gcgttaggat gtaagaaagt tccagtgcaa
    18961 gaaatccatg aaacacaata ttcaattagt acatggcaac ataaagttcc atttggtgtg
    19021 tggtgggaaa cgttacaaca agaacatcgc ttgccatgga ctactgagac aagacaagaa
    19081 gcgccattta ttacaatgtg tcatggtgat acagaacaat atttgtatac aaaagattta
    19141 ggcgaagcac attttcaagt atgggaaaag gttgtcgcaa gttatagtgg ttgttgttct
    19201 gtagagagaa ttgcacaagg tacatatcct tgtctttctc aacaagatgt actcatgaag
    19261 tatcagccat tgagttataa ggaaattgaa gcggttgttc ataaagggga aactgtgcca
    19321 gcaggtgtga cacgctttaa tatttcagga cgatgtctta atcttcaagt accactggca
    19381 ttacttaaac aagatgatga tgttgaacaa ctgcgcaatt ggaagcagtt tttagcagat
    19441 aagtttgcca atatgagatg ctatactgaa aaagtatact tggtggagca atagttttac
    19501 tgtgatgttg agggaaatat gatgatttag cgtattgata gcgaaaatat aataaaacaa
    19561 tatagtgtgg agaacttttg atattttata aatattgaag ttctccattt ttgtattttg
    19621 catataaaaa ttaaataaaa taaggtatat taaggtaaag tataaatttt aaataaatgg
    19681 ggaatgagta tgagctcaat tataggaaaa atagcaattt ggataggcat cgtagctcaa
    19741 atatatttta gtgtcgtttt tgttaggatg atatctatta atattgctgg aggatctgat
    19801 tacgaaacaa tttttttatt aggattaata ttggctcttt tcactgtttt accaaccatc
    19861 tttactgcga tttatatgga aagttactct gtaatcggag gtgcactttt tattgtttat
    19921 gctattattg cactgtgttt atataatttc ctttcgtcaa ttttatggct gattggtggt
    19981 attttgctga tttggaataa atactcaaaa gatgaatcga cagacgaaaa tgaaaaagtt
    20041 gatattgaaa gtacagagaa tcaatttgaa tctaaagata aaatcactaa agaataaaga
    20101 gaatatttaa ggtaaagtat aaattttaaa taaatgggga atagacatgg aaaaaaatgt
    20161 agaaaaatca ttcataaaga taggtttata ttttcaaata gcttatatag tactcatggc
    20221 tataacttta tgtgggtttg taatttgcta tggactaatt ttcggccttt tctatttatt
    20281 atcaggtagc agagctgatt atttaatagt aacaatagtt atatcggcaa taatttctat
    20341 atttgtaatt atactttcaa tcgtacctgt catcgtattg gcatctgact tatttaaaga
    20401 aaggatttca aaaggtgtca tattaattgt attggctatt atcgctttag tattatgcaa
    20461 ctttgtatct gcaatactct ggtttgtttc agccatatct attttaggta gaaaaaaatt
    20521 agtagctgca gcagatacta ccactattca aaaaagtaaa gggaacgcaa atcaagcatc
    20581 acataaagac acgtgtaaaa aggaacttga tagtcaagac atgatggaac atcctgaggt
    20641 taaaaatccc acgactaaaa accttgaagg atttaacgaa gaaatacata aagatgaagc
    20701 tacaactaaa gttgtcagtg ataacacgga accgcctatt gaatcaaaag accatgtctc
    20761 gaaaaaagat tgatgacaaa ctaatcgaga gacttaaaaa aataatattc aacataagaa
    20821 cttttaaaac gacatttaaa cgcattgcca atcactaatg gtagtgcgtt taactatacc
    20881 ttaaatatct gaatattttg ttaaatggag ctacctttgt tgtactattc aaatgaagag
    20941 gagtaaaatg taattaaagg aaagaaattt gaggagtgat ctttatgaca aacaacaaag
    21001 tagcattagt aactggcgga gcacaaggga ttggttttaa aattgcagaa cgtttagtgg
    21061 aagatggttt caaagtagca gttgttgatt tcaatgaaga aggggcaaaa gcagctgcac
    21121 ttaaattatc aagtgatggt acaaaagcta ttgctatcaa agcagatgta tcaaaccgtg
    21181 atgatgtatt taacgcagta agacaaactg ccgcgcaatt tggcgatttc catgtcatgg
    21241 ttaacaatgc cggccttgga ccaacaacac caatcgatac aattactgaa gaacagttta
    21301 aaacagtata tggcgtgaac gttgcaggtg tgctatgggg tattcaagcc gcacatgaac
    21361 aatttaaaaa attcaatcat ggcggtaaaa ttatcaatgc aacatctcaa gcaggcgttg
    21421 agggtaaccc aggcttgtct ttatattgca gtacaaaatt cgcagtgcga ggtttaacac
    21481 aagtagccgc acaagattta gcgtctgaag gtattactgt gaatgcattc gcacctggta
    21541 tcgttcaaac accaatgatg gaaagtatcg cagtggcaac agccgaagaa gcaggtaaac
    21601 ctgaagcatg gggttgggaa caatttacaa gtcagattgc tttgggcaga gtttctcaac
    21661 cagaagatgt ttcaaatgta gtgagcttct tagctggtaa agactctgat tacattactg
    21721 gacaaacaat tattgtagat ggtggtatga gattccgtta ataatcatcc actaatgata
    21781 aataaatcct tattgttaag tttaatcact tagcagtaag gattttttag tgcacttaga
    21841 agggagtgta ttggtagaaa attaataagc gaagttctta agtgagttat gatgtcacag
    21901 tctaatgcat cagttgaaag cattattagt attaacacac ccaagatatt ataaaacatc
    21961 acaaaaacac cactatctaa tttatctcaa taaaaattca caaagttatc tcattttatt
    22021 tttataaat
//
LOCUS       DANJFZ010000036        21784 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.036, whole genome shotgun sequence.
ACCESSION   DANJFZ010000036 DANJFZ010000000
VERSION     DANJFZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.036"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(149..709)
                     /locus_tag="PWN45_001182"
     CDS             complement(149..709)
                     /locus_tag="PWN45_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL0622960.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    767..1068
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1112..3742
                     /locus_tag="PWN45_001183"
     CDS             1112..3742
                     /locus_tag="PWN45_001183"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL0622961.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            3755..5026
                     /locus_tag="PWN45_001184"
     CDS             3755..5026
                     /locus_tag="PWN45_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL0622962.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            5296..6003
                     /locus_tag="PWN45_001185"
     CDS             5296..6003
                     /locus_tag="PWN45_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL0622963.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            6000..6686
                     /gene="radC"
                     /locus_tag="PWN45_001186"
     CDS             6000..6686
                     /gene="radC"
                     /locus_tag="PWN45_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL0622964.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            6754..7038
                     /locus_tag="PWN45_001187"
     CDS             6754..7038
                     /locus_tag="PWN45_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622965.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(7155..7352)
                     /locus_tag="PWN45_001188"
     CDS             complement(7155..7352)
                     /locus_tag="PWN45_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622966.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            7512..7985
                     /locus_tag="PWN45_001189"
     CDS             7512..7985
                     /locus_tag="PWN45_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDL0622967.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            8059..8178
                     /locus_tag="PWN45_001190"
     CDS             8059..8178
                     /locus_tag="PWN45_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622968.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            8378..9220
                     /gene="mreC"
                     /locus_tag="PWN45_001191"
     CDS             8378..9220
                     /gene="mreC"
                     /locus_tag="PWN45_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL0622969.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            9220..9750
                     /gene="mreD"
                     /locus_tag="PWN45_001192"
     CDS             9220..9750
                     /gene="mreD"
                     /locus_tag="PWN45_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL0622970.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    9777..9904
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9917..10225
                     /gene="rplU"
                     /locus_tag="PWN45_001193"
     CDS             9917..10225
                     /gene="rplU"
                     /locus_tag="PWN45_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL0622971.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            10231..10551
                     /locus_tag="PWN45_001194"
     CDS             10231..10551
                     /locus_tag="PWN45_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL0622972.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            10563..10847
                     /gene="rpmA"
                     /locus_tag="PWN45_001195"
     CDS             10563..10847
                     /gene="rpmA"
                     /locus_tag="PWN45_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL0622973.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            11202..12494
                     /gene="obgE"
                     /locus_tag="PWN45_001196"
     CDS             11202..12494
                     /gene="obgE"
                     /locus_tag="PWN45_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL0622974.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            12507..12962
                     /locus_tag="PWN45_001197"
     CDS             12507..12962
                     /locus_tag="PWN45_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDL0622975.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            12976..13578
                     /gene="ruvA"
                     /locus_tag="PWN45_001198"
     CDS             12976..13578
                     /gene="ruvA"
                     /locus_tag="PWN45_001198"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL0622976.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            13610..14614
                     /gene="ruvB"
                     /locus_tag="PWN45_001199"
     CDS             13610..14614
                     /gene="ruvB"
                     /locus_tag="PWN45_001199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL0622977.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            14616..15641
                     /gene="queA"
                     /locus_tag="PWN45_001200"
     CDS             14616..15641
                     /gene="queA"
                     /locus_tag="PWN45_001200"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL0622978.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            15664..16803
                     /gene="tgt"
                     /locus_tag="PWN45_001201"
     CDS             15664..16803
                     /gene="tgt"
                     /locus_tag="PWN45_001201"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL0622979.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            16822..17082
                     /gene="yajC"
                     /locus_tag="PWN45_001202"
     CDS             16822..17082
                     /gene="yajC"
                     /locus_tag="PWN45_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL0622980.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            17357..19636
                     /gene="secDF"
                     /locus_tag="PWN45_001203"
     CDS             17357..19636
                     /gene="secDF"
                     /locus_tag="PWN45_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDL0622981.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            19839..>21784
                     /gene="recJ"
                     /locus_tag="PWN45_001204"
     CDS             19839..>21784
                     /gene="recJ"
                     /locus_tag="PWN45_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL0622982.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYF"
BASE COUNT         7728 a         2984 c         4147 g         6925 t
ORIGIN      
        1 ctatttttag attaagatat ctaatattag cagagcatta acttggaact ttaacttatg
       61 atttgaataa attttcacat taaaaaacga gacagtctat tagttaaaaa tagatgatgt
      121 ctcgtttact ttatgatgct atagcaaatt aattatgttt aggctttgat ggacaatctt
      181 gtaaatgtgc atcatataaa ccggctgctt ctaaaaacga aaatactgtt actggaccta
      241 aaaatttaaa cccgtattgt tttaaatctt tagatagttg tgttgcagta tcatcaactg
      301 tgatacgatc agaagcatgt tcatactgca aatctttagg cttaccattt acatatgacc
      361 ataaaaattt actaaaacta ccatatgctt gttcaatttt taaataccct tgagcttgat
      421 taacaattgc ttctaatttt ttacgatgat gaacgatatt tggaaaagtc attaagcggt
      481 cgatatcttg agcggtcatt tgtgctacct tttctggttc gaaatcataa aatgcttctt
      541 cataggcttc tttctttttt aaaatagtta accaagatag cccagcatgt tgtgattcta
      601 atgctaaaag tttaaacaat gccttgctat catagagcgg ttgtccccat acatgatcat
      661 gatagtctaa gtagactgga tctttagtac caaatgcgca ttcattcatt ttattcccac
      721 ctatacattg acttattttg tcaatcatta tactataagt taagagttta atataggatt
      781 tgggaagagt aagttcaaat tattttaaag agagcatatg atttggtgtg agtatgtaaa
      841 tgatttttac tgaaggtagc ccacagagta tcttagctga acgtgattaa tagtaggcta
      901 agacgtgttt gagcgttaat cataatctag agtgcttgaa gagatgaagc atttcaaatt
      961 tcacgatgat agtattggta tgattattcc aattatgtta aaagagtgaa tttgatatta
     1021 atcatccttc ttgaatatag gtggtaccac ggaatatccg tcctatttgt atataggatg
     1081 gatattttta tttttttagg aggtatagca aatggaaatg aaaccaaaat atgatcctcg
     1141 tgaagttgaa gcgggacgtt atgaagaatg ggtaaagaat ggttatttta aaccgtcaga
     1201 agataaatca aaagaaacat atacaattgt tatcccgcca ccaaatgtaa ctggtaaatt
     1261 acatttagga catgcatggg atacgacttt acaagatatc attacacgta tgaaacgtat
     1321 gcaaggatac gatacgttat acttaccagg tatggatcat gctggtattg cgacacaggc
     1381 aaaggtagaa gctaaattaa atgaacaagg aataactaga tatgatcttg gtcgtgaaaa
     1441 gtttttagaa caggcatggg attggaaaga agagtatgcg tcatttattc gtgcgcaatg
     1501 ggctaaatta ggtctaggtt tagattatag tagagaacgt tttactttag atgaaggttt
     1561 aagtaaagca gttaaaaaag tttttgttga tttatacaat aaaggaatta tttatcgtgg
     1621 cgaacgtatt ataaattggg atcctaaagc acgtacagct ttatctgata ttgaagtaat
     1681 acatgaagat gttcaaggtg cgttttatca ttttaaatat ccttacgctg atggtgaagg
     1741 ttttattgaa attgcaacaa caagaccaga aacgatgtta ggtgatacag cgattgttgt
     1801 taaccctaat gacgaacgat acaaagatgt aatcggtaaa actgttatat taccaatcgt
     1861 aggacgcgaa ctgcctattt tagcagatga gtatgttgat atagacttcg gttctggtgc
     1921 tatgaaagtg acaccagcac atgaccctaa tgattttgaa attggtcaaa gacatcaatt
     1981 agaaaatatt atcgttatgg atgaaaatgg taaaatgaac gacaaagcgg gtaaatatga
     2041 aggtatggac cgttttgatt gtcgtaaaca gctagttaaa gatttaaaag aacaagattt
     2101 agttatcaag attgaagatc atgttcattc tgtaggtcat tcagaacgat ctggcgctgt
     2161 tgttgaacca tatttatcaa cacaatggtt tgtgcgcatg gaagacttag cgaaacgttc
     2221 attagataac caaaaaacag atgatcgtat tgatttttat ccgcaacgtt tcgaacatac
     2281 atttaaccaa tggatggaaa atattagaga ttggacgatt tcaagacaat tatggtgggg
     2341 tcatcaaatt ccggcttggt atcataaaga aacaggcgaa atatatgttg gagaagaagc
     2401 gccaactgat attgaaaatt ggcaacaaga tgaagatgta ttagatacgt ggttctcaag
     2461 tgctttatgg cctttctcta cgttaggttg gcctgattta gaaagtgaag actttaaacg
     2521 atactaccca acaaatgcct tagttacagg ttacgatatt atctttttct gggtagcacg
     2581 catgatattc caaggcttag aatttacaga tcgtcgtcca tttaatgatg tattattaca
     2641 cggtttagtt cgtgctgaag acgggcgtaa gatgagtaaa tcattaggta atggtgtgga
     2701 tccaatggat gttattgacg aatacggtgc tgatagcttg cgttacttct tagcaacagg
     2761 ttcatctcca ggacatgatt taagatactc aactgaaaaa gttgagtcag tgtggaactt
     2821 tatcaataaa atctggaatg gggcacgttt cagtttaatg aatatcggtg aagactttaa
     2881 agttgaagat atcgatttaa gtggtaactt atcattagca gataaatgga ttctaacacg
     2941 tttaaatgaa acgattgcaa cagttactga tttaagtgac aaatatgaat tcggcgaagt
     3001 tggacgtgca ttatataatt tcatttggga tgatttctgt gattggtaca ttgaaatgag
     3061 taaaattcca atgaatagta atgatgaaga acaaaaacaa gttacacgtt cagtattgag
     3121 ttatacttta gacaatatta tgagaatgct acatccattc atgccatttg taacagagaa
     3181 aatatggcaa agtttaccac atgaaggtga cacaattgtt aaagcttcat ggccagaagt
     3241 gcgtgaatca ttgatttttg aagaaagtaa acaaacaatg caacaacttg ttgaaatcat
     3301 taaatctgta agacaatcac gtgtagaagt aaatacgcca ttgtctaaag aaatacctat
     3361 tttaattcaa gctaaagata aagaaattga aacaacttta tcacaaaaca aagattattt
     3421 aatcaaattc tgtaatccta gtaccttaaa tattagcact gacgtggaaa ttcctgagaa
     3481 agcaatgaca tcagttgtaa ttgcaggtaa agtggtatta ccattagaag ggctaattga
     3541 tatggataag gaaatcagcc gtttggaaaa agaattagct aaacttcaaa gcgaattaga
     3601 tagagtagat aaaaagctct ctaatgaaaa ctttgtaagt aaagcacctg aaaaggttat
     3661 aaatgaagaa aaacgtaaaa aacaagatta tcaagaaaaa tatgatggtg tgaaggcaag
     3721 aattgaacaa ttaaaagcat aggagttagt aacaatgaat tacctagaga gcttgtattg
     3781 gatacacgaa agaactaaat ttggcatcaa accaggtgtt aaacgtatgg aatggatgct
     3841 agcacaattt aataatcctc aaaataacat taagggtatt catgtaggtg gcacaaatgg
     3901 taaaggctct acagttgctt accttagaac agctttagtt gaaaatggtt atgaagtagg
     3961 tacatttacg tcgccgttta ttgaaacatt taatgaacga attagtctaa atggtgtgcc
     4021 aatatcaaat gacgctattg tagaattagt atcacgtatt aaaccagtaa gtgaaatgat
     4081 ggaacgtgaa acagatttag gtgttgcaac tgaattcgaa ataatcacag cgatgatgtt
     4141 tttatatttt ggtgaaatac atcctgttga ttttgtcatt gttgaggctg gattgggtat
     4201 aaagaacgat tcgacaaatg tctttacacc ggttttatca atcttaacta gtatcggtct
     4261 agaccataca gatattttag gtggtactta tctagatatt gctagggata aaggcgcgat
     4321 tataaagcct aacgttccag tgatatatgc tgttaaaaat gaagatgcat taaaatatgt
     4381 tcgtgaacgc gcaattgaac aacatgcaaa gccaattgaa ttagatagag aaattgttgt
     4441 tgtatcgcaa aatgatgaat ttacttaccg ttataaagat tatgaattag aaacaatcat
     4501 tttaagcatg ttaggtgaac atcagaaaca aaatgctgca ttagccataa cagctcttat
     4561 tgaattaaat gaacaaggat taattgaatt agatttcaat aagatgatag acggtattga
     4621 atcagttcgt tggactggac gtattgagca ggtgcatgac aaacctttaa tcattttgga
     4681 tggcgcacat aattcagaga gtatagatgc tctaattgat acaattaaac agtaccatga
     4741 taaagaaaaa gtagatattt tgttctcagc aataaacgga aaaccgatta acgagatggt
     4801 caaacattta agtttaattg cgcatacgtt ttatgcaact gaatttgatt ttccgaaagc
     4861 gttacgcaaa gaagaaattg taggtagtat tgaaaatgat gaaatacaat tagtagatga
     4921 ctacgttgaa tttataaaaa attatcaagg tgatacatta gtaattaccg gtagtctgta
     4981 tttcataagt gaagttaaat caaaattaga ctttgaagca ttataaaact aaaactaaaa
     5041 tagccatgaa gaatctattt aatcaatagg tagagcttca tggttatttt ttatgctcta
     5101 aatcgtatgt gtttataagg ttcaatttct tgccattatt aattttgccg agcagtaatg
     5161 ggttgttaaa gtatccatca attatgctta gcaatattga aaaatttaaa tgaaaaagcg
     5221 gttcaattct tgaaaaatag tgtatattta cattttggta ataactaact ataatttaca
     5281 taaggaggtt gtcatttggt agtattgtta tcttatagtt gcagttgtat ttttagtttt
     5341 ctatatcaat ttatatctat agaagaaacg tcatttgatt atttacatag aagatcgaaa
     5401 tgtgattatt gtaattcgtc actcaaatgg tatgaattaa tgccgattat tagtttttta
     5461 ttattaaaag ggcgatgtcg aaactgtcga aagcgtattt ccctaacaca tttcttaggg
     5521 gaaacctttg ctttaatacc tatcgtcttt attaagtatg atttcacata cgtaaatgct
     5581 acgctattta taactacata tgtttttctg cttattttta ctatgaccga tatcacttct
     5641 ttaatgttag attgtcgctt aattataatt tattgtatcg tttctctctc gttaagtatg
     5701 atttatccag tagcttttat cattattagt atgaccacgc atatattcta ctttttattt
     5761 cgggcatata ttggttatgg tgacgtttta ctaatatctg cactttcttt gtttttccct
     5821 ctccaattca ctatttatgt cattttattt acatttgtca ttgctggttt agttgcttta
     5881 attaccatga tatttaagcc gattaaacta ttaccccttg ttccatttat atttatttcc
     5941 ttttttatca attcactttt ttataatgat atccatcaat ttttaggagg cgtatatttt
     6001 tgaaaattaa agaaatggta acttcagaaa tgccaagaga acgtttgtta agccatggtg
     6061 caaaaagtct ttcgaataca gaattattag ctatattaat taacaccgga agaaaaggat
     6121 tctcgagcat agacattagt aatgaactgc ttaaatctgc ttcgaatctg aatgaattga
     6181 aaaaatcttc aattaacgat ttgataaaag ttaaagggat tggattacaa aaagcaatta
     6241 ctttaaaagc agcatttgag ttaggggaaa ggatggggag aagagctgaa aataatcgta
     6301 taaaaataac gcaaccaagt gatgttgctg attatatgat tccaacaatg aaagatttaa
     6361 cacaagaaca ttttgtcatt ttattgttaa attcaaaaaa tgtagtgatt aaagaaacct
     6421 gtgtttttaa aggtacatta aatagttcga ttgtacatcc acgtgaaatt tttagtattg
     6481 cggtgagaga aaatgccaat gcaatcatcg cagttcataa tcatccatcc ggtgatgtaa
     6541 cgccctcaca agaagatatc ataacaacaa tgaggttgaa ggagtgtggt ttgattttag
     6601 ggatagattt attggatcat attataatcg gtgataatag atttaccagt cttgtagaag
     6661 cgggttactt tgatgaaaat gattgaagtt gatagaatta atgacgtatc ttgtgtataa
     6721 tacctacgaa gtactttcat tggaggaaaa atagtgactc tatttattat tatcggggtt
     6781 ctcgtgccaa tggtttatac catgcagtta aatattaaaa atgaacctgt aacaaagcgc
     6841 aatcttttaa taacattagc tttatctacg ttaggtattt tagtaaccgc gttagcaggt
     6901 gtaatcgtta cgaaacaagc ttttccttta ttaagtgtag caattggctc aatttttact
     6961 ggaatcgttt ggggcctttt actaagtggt agctacgcgc tgatacgatt tttatctaac
     7021 gcatttgggc gtaagtaata gtaatctgtt catcaagtag tatccgtgct tgaaaacaaa
     7081 ctaaaactcc taatgtggaa ctagttaaaa aattctaaac tagtacatta ggagttttgt
     7141 tatgcagaat aaatttaatt gttaaattga aagtgcggta aaaatccact atttatttga
     7201 aaaaaatcga gcaaccaaat taaaccttga tatcctaagt aaatacataa taaaacaagt
     7261 ccgacataaa ttaaaaatcg caaaatagaa agtccaactc taaaaaggag gatgactaat
     7321 agtgctatta aaataattgt tagtatactc aatgctacaa acctcctaat acgcttttaa
     7381 atcataacaa aattataagc tattttaaat gaacgtgcat cagtcctaag atatatcatg
     7441 attaaggctg aagactgagg aaaatataca taataaaata tataattaga tgaagacatt
     7501 ggaggcgaac tatgagattg atatttagta tgattaaaaa cattatagca gtaatagcaa
     7561 ttgtgataat tgtttatatc gccttgaaat acgcaccttt tttaaaagaa caagattgga
     7621 atcctgtaaa tcacgataat gatcaaatga atcaagttac gcaacccact aatgatgccc
     7681 aacatgttta tgtaagtggt gagaaatatt cattaaaaga aaatgattta ataaagaatg
     7741 taccattaag ccaaattaaa aatgtattta aaatgataga taaacaagag tttatggctg
     7801 tgtctggaat gaatcgcatg gcttataatg atcaatatat tataggtcaa agaggagacg
     7861 aatttattct ttataaattt ggagatgagt caatgcgtgt ttacaatact gaatttgaaa
     7921 tgcaacaaga cttaaatgaa ttagggcaaa atttacaact aaaatctgaa aatgcttatc
     7981 aataggaaat tattgcataa gaaatatatt ttagttatcc tgtcactaga ataaatttaa
     8041 aaggttggtg tagttcaaat ggcagaacaa tcaaaacaga aacaagctaa tgaacaacaa
     8101 aaggcccaga acttattcgc acgttggaga caacttcaga attcgaacag tgaatcctcg
     8161 aacgatacaa acaaataatt ttaaaattta aatcgattat ttaaactact agtgactgga
     8221 atgtttgcta gtagtttttt tgtttttaaa atgccacaaa ttttttacgg agttgttcat
     8281 tttcaaagta attcgtagtt agtgctttat ttcataatgg gagtaatata aaatagaaaa
     8341 ggatatacaa tattttgaca taatagaggt gttctgggtg cttaagtttt ttaaaaataa
     8401 caaattaatt gttgttttat gtgcaattat cgtttttatt gcattaattg ggctgtccat
     8461 acgttcacaa tctcaatcac ctcctgaaca atatataggt gattctgtgt cttttggaca
     8521 acgagttgtg agttatccag ttaattttgt tgctggtacg attggggact tttttaaaaa
     8581 aggagattct aaagaatcta aaaataagat tagccagtta gaatctaaga accaacaatt
     8641 agaagcggaa aatgaaaaat taaaaaaaga gcttgattta aaagatattt caaaatttga
     8701 tcctatttct actacggttt tggcaagaaa tccggatcag tggatgaata caattgtaat
     8761 tgataaggga tctaaatcag gtataacttc aaatatggct gtgatgacat cacaaggttt
     8821 tgttggaaga gttactaaag ttaataaatt ttcttcacaa gttgatttaa tctcaactaa
     8881 tacacgtgcg ggtaaattat ctgtaaatat acaacacggt tctaaaaata tatttggttt
     8941 aattgatcgt tatgatgaaa agaactcaga acttgtaatt agtgacatta ataatagaga
     9001 taatatctca aaaggtgata aagtcgttac aagtggatta gctgatcaac taccaagtaa
     9061 tttatatata ggagaagtga ctaaggttca aaatgatcaa tacggcttag ctaaagaagt
     9121 tagggttaag actggtgcgg acttaacaga tttgagtcat gtttatgttg caaaaagaga
     9181 tcctaaaaca attcctgatg atgaaagcag ggataaataa tgcgtacact gtattatttt
     9241 ttgataggta ttttactatt ttatatagat actgcaattg ggcttcttat tccaatgcac
     9301 ataggtaagt ttgaacttgt atttgtacca caccttacat ttatgtatat tttaatgatg
     9361 gtagtgtatc gtggctttgg cgtatcatta ttgctcagca tatttttagg cgtaatgact
     9421 gatgtatact ttggtagtat ttatggagtg tacttatttg gctatatatt gtttttagca
     9481 cttatagatc gattctttaa aattttttac aaagatcatt cgatgttatt cattattata
     9541 ttagccagca ccttattatt agaagtctat gtggcattaa tatacggtat gttaggattc
     9601 attcaatttg atattattca ttttgtagtc tttagattat tgccaacatt aattatgaat
     9661 tttgtactgt tgattatgct ttatccgttg attataaagt tccttaaaaa aacaaacaat
     9721 gacattgaca tgaaacgtcg tcaatggtaa gatgcaatag ttgagtagtt ataagtagct
     9781 acatacaacc gctcaaatat aggttcaaga acatttttaa tgaagaaagc gattttccca
     9841 cagagtgcaa acgcttgact caataacgaa atgtcaccta tgtatggcgt gacttattat
     9901 acaggaggtg caaagtatgt ttgctattat tgaaacaggt ggaaaacaaa tcaaagtaga
     9961 agaaggtcaa gaaatcttcg ttgaaaaatt agacgtaaac gaaggagata cttttacatt
    10021 tgataaagta ttatttgtag gtggagattc agttaaagtt ggagcgccaa cagttgaagg
    10081 tgcaacagtt actgctactg ttaataaaca aggtcgcggt aaaaaaatca ctgtattcac
    10141 atacaaacgt cgtaaaaatt caaaacgtaa aaaaggccat cgtcaaccat acactaaatt
    10201 aacaatcgat aaaatcaacg cgtaattatt atgattactg ttgatattac agttaatgat
    10261 gaaggcaaag taacagacgt tattatggat ggccatgctg accatggtga atatggtcat
    10321 gatatcgttt gtgctggagc ttcagctgta ttgtttggta gtgttaatgc gattatagga
    10381 ttgacatctg agagaccaga tatcaattat gacgacaatg gtggtcattt tcatataaga
    10441 agcgttgata caaacaacga tgaagcgcaa ctaattcttc aaacaatgct tgtgtcttta
    10501 caaactattg aagaagaata taatgagaat attagattaa attataagtg aggtgcattc
    10561 cgatgttaaa attaaactta caattcttcg catctaaaaa aggggtaagt tctacaaaaa
    10621 acggacgtga ctctgaatca aaacgcttag gtgctaaacg tgctgacggt caattcgtaa
    10681 caggtggttc aattttatat cgccaacgtg gtactaaaat ttaccctggt gaaaatgtag
    10741 gtcgtggtgg cgatgataca ttattcgcta aaatcgacgg cgttgttaaa ttcgaacgta
    10801 aaggtcgcga caaaaaacaa gtttctgtat atgcagtagc tgaataattt tgtctagtta
    10861 acaccagaag tgaatcttct ggtgtttttt actttttata aaatgttttt cattattatt
    10921 ttcattatgt tattaaaaaa tgggaatttt agacgttata ctaaatgtgc aactgtataa
    10981 gagcccctaa tcactaaact ataaggggga caaaggaata cagttgcagc gtttaaagaa
    11041 taaactgtac cacaattggt gctgagaaat ataagtattt taaagcaaag atttataaaa
    11101 gtaactgcat aagagcccct aattatttac aatatataag gggctctaat atgctataat
    11161 tattgggaaa atgaaaatta tatgtaaaag aggtgagata tatgtttgtc gatcaagtca
    11221 aaatatctct taaagccggt gatggtggta atggtattac cgcatacaga agagaaaaat
    11281 atgtaccatt tggtggacca gctggcggtg acggtggtaa aggtgcttca gtcgtatttg
    11341 aagtggatga aggtttaaga acgttattag attttagata tcaacgtcat tttaaagcaa
    11401 gcaaaggtga aaatggccaa agtagtaata tgcatggtaa aaatgcggaa gatttagtat
    11461 taaaagttcc acctggtaca attattaaaa atgttgaaac agacgaagtg ttagcagatc
    11521 ttgttgaaga tggtcaaaga gctgtagtag cgaagggcgg tcgaggtggc cgaggtaatt
    11581 cacgttttgc aacacctaga aaccctgcac ctgacttcag tgaaaaaggt gaaccaggtg
    11641 aggaattaga tgtatcttta gaattgaaat tattagctga tgtaggatta gtaggtttcc
    11701 ctagtgtggg taaatcgact ttattatcta tcgtttcaaa agctaagcct aaaattgggg
    11761 catatcattt tacaacgatt aaaccaaatc taggtgttgt ttcaacgcct gatcaacgta
    11821 gttttgttat ggcagattta ccaggtttaa ttgaaggtgc atctgatggc gttggattag
    11881 gacatcaatt tttaagacat gtagagagaa caaaagttat tgttcacatg attgatatga
    11941 gcggttctga aggtagagaa cctattgaag attataaagt cattaatcaa gaattagctg
    12001 cgtacgagca acgtttagaa gatagacctc aaatcgtagt agctaacaag atggatttac
    12061 ctgaatcaca agataattta aacttgttta aagaagaaat tggcgaagat gtgccagtta
    12121 ttccagtttc aacaataacg cgtgataata ttgatcaatt attatatgca atagcagata
    12181 aattagaaga atataaagat gttgacttca cagttgaaga agaggagtca gttggcatta
    12241 accgagtatt atataaacat acaccgtcac aagataaatt tacaatttca agagatgatg
    12301 atggtgctta tgtggtaagt ggtaatgcta ttgaaagaat gtttaaaatg actgacttta
    12361 acagtgatcc agcagtacgt cgatttgctc gtcaaatgcg ttcgatgggt attgatgatg
    12421 cgcttagaga acgtggttgt aaaaatggtg atatcgttag aattcttggc ggagaatttg
    12481 aattcgttga ataggagcga aacatgatgg acaataaaga ttataaaaag ttttatttaa
    12541 ttagagaaga tgtcttgcct gaatccgtgg ttaaaacatt gaagattaaa gatgccttaa
    12601 aaagtgatcc gacattgtcc atttatgatg ccgttaaaca gtttgatcta tctagaagtg
    12661 ctttttataa atatagagaa acgatatttc cagtagacga taaaatgctt gaccatcgag
    12721 aatttacatt aattttatat gtaactgata ttgttggtat gttggcacgt gtactagatg
    12781 ttatatcaaa gttagaacta tctgtattaa cgattcatca aagtattcca atggaagaaa
    12841 aagcaacaat aacattatca ctgaatgcta aatctaaaga aacttcagta gaagatgtta
    12901 ttggcgcttt gagaaattta gattatgtat caaaagtaga attaattagt atgagtatgt
    12961 aaggatgtgc ctataatgta cgcgtatgtc aaaggtaagt taacacattt atatcctaca
    13021 cacgtagttg ttgaaactgc tggtgttggt tatgaaattc aaacaccaaa ttcttatcgt
    13081 tttcaaaagc atctagatca tgaagtttta attcatacat ctttaattgt tcgtgaagat
    13141 gcacaattat tgtatggatt tagtagtgaa gaagagaaag atatgttctt gagtttaatt
    13201 aaagttactg gtattggtcc gaaatcagct ttagctattt tagcgacaag tacgcctaat
    13261 gaagtaaaac gtgccattga aaatgaaaat gatacgtatt taactaaatt cccaggaatt
    13321 ggtaagaaaa cggcaagaca gattgtctta gatttaaaag gtaaagtgaa aattactgaa
    13381 gaagatagcg attcattatt acaagtagac gctacttcga cggtgcaaga tcaattcgtg
    13441 caagaagcaa tgttagcgtt agaagcatta ggttattcta aacgagagct tgcaaaagtt
    13501 gagaaaacgt taaataaaaa taaatatgac tcagttgatg aagctgttaa ggcaggtctt
    13561 caattagttg tatcttaatt ttaaatagat taatagggga agtgttgtca tgaatgagcg
    13621 tatggttgat caatcaatgc atagtgaaga aactgatttc gaattgtcgc ttagacctac
    13681 gagattacga caatatattg gtcaaaattc aataaaaagt aatttagaag tatttattaa
    13741 agcggctaaa cttcgtcatg aaccattaga tcatgtattg ctttttggcc cccctggatt
    13801 aggtaagaca acattatcta atatcattgc caatgaaatg gaagttaata tacgtacagt
    13861 atcagggcct tcattagaaa gacctggtga tttggctgca attttatcag gacttcaacc
    13921 tggagatgtt ttgtttattg atgaaataca cagactgagt agtgttgttg aagaagtgtt
    13981 ataccctgca atggaagatt tctttttaga tattatcatt ggtaaaggcg atgaggctag
    14041 aagtatccgt atcgacttac ctccattcac tttggtaggt gcaacaacgc gagctggcag
    14101 cttaacaggt ccactaaggg atcgatttgg tgtgcactta agattagaat attataacga
    14161 atcagattta aaagaaatca ttattagaac agctgaggtt ttaggcacag gtattgatga
    14221 agaaagtgcc attgaacttg ctaaacgttc tagagggact ccaagagtag caaatcgact
    14281 attgaagcgg gtaagagact tccagcaagt gaatgaagat gaacaaatat acattgaaac
    14341 aacgaagcac gcattaggtt tacttcaagt tgatcaacac ggactagatt acattgatca
    14401 taaaatgatg aactgtatta ttaagcagta taatggcgga cctgttggtt tagatacgat
    14461 tgccgtaaca attggtgaag aacgtattac aattgaggac gtttatgagc catttcttat
    14521 tcagaaaggc tttttagaac gtacgccacg tggcagaaaa gcaacaccat tagcttatga
    14581 acattttgca aagtcgaatg aggagagaga ataacgtgaa tattgaagaa tttgactatg
    14641 acttaccaga atcattaatt gctcaaacgc ctttaaaaga tcgtgatcat agtcgtttat
    14701 tagtcatgga tagagaaact ggtgaaatga aacatttaca tttcaaagat atcattgagt
    14761 attttagacc tggtgataca ttagtgctta acgatacgcg agtaatgcca gctagacttt
    14821 ttggtttaaa agaagaaact ggtgcaaaag ttgaaatgtt aatgttaact caaattgaag
    14881 gtaatgattg ggaagtctta ctgaaaccag ctaagcgtat taaagttggt aataaattga
    14941 attttggtaa tggcaaaatt atagctgaat gcataaaaga aatggatcaa ggtggacgca
    15001 tcatgcgttt acattatgaa ggtattttac aagaaagatt agatgaatta ggggaaatgc
    15061 cactgccacc atacatcaaa gaacgtttag atgatccaga tcgttatcaa acagtttacg
    15121 ctaaagaaag tggttcagcg gcagcaccaa cagcaggatt acattttact gatgagttat
    15181 taattgaaat taaaaataaa ggtgttaata tcgcatttgt tacattacat gttgggttag
    15241 gtacgtttag accggtgagc gtagacgatg tgaatgacca cgaaatgcat agtgaatatt
    15301 atcaaatgac acaagaaaca gctgatttat taaatgatac taagtccaaa ggacatcgca
    15361 ttatatcagt tggtacaact tcaacacgta cacttgaaac aattcgacgc gatcatgata
    15421 aatttgttga aacgagtggc tggactaata tatttattta tccaggattt gattttaaag
    15481 caattgatgg ccagattact aattttcatt taccaaaatc aacattagtt atgctagtat
    15541 cagcgtttag tagtcgtgaa aatgttctga atgcttataa aacggcagta aatttagaat
    15601 atagattctt tagttttggc gatgcaatgt taattatata aaaagaatgt gaggattttg
    15661 aatatgcctg cagtaacata cgaacacatt aaaacttgta aacaatcagg tgcgcgttta
    15721 ggtatcgtgc acacaccaca cggttcattt gaaacaccta tgtttatgcc agttggtact
    15781 aaagcaaccg ttaaaacaat gagtccagaa gagttaagac aaattgaagc aaaaatcatt
    15841 ttgggcaaca catatcattt gtggttacaa cccggaaatg atattatcaa acacgctggg
    15901 ggattacata aattcatgaa ttgggatggt ccgattctta cagattcagg cggtttccaa
    15961 gtgtttagtt taagtaattt acgtaaaatt acagaagaag gcgtggaatt tagacatcat
    16021 actaatgggt ctaaattatt tttgagtcct gagaaatcaa tgcaaattca aaatgattta
    16081 ggatctgata ttatgatggc atttgatgaa tgtccaccga tgcctgctga atatgattat
    16141 gtaaaaaaat ctattgaacg tacaacacgt tgggcgaaaa gatgtctaga tgcacaccaa
    16201 agacctgaag atcaagcatt gttcggcatt atacaaggtg gcgaatatga agatttaaga
    16261 gaacaaagtg caaaggattt agtagaatta gattttcctg gttatgcaat cggcggtttg
    16321 tcagttggtg aacctaaacc ggttatgtat aaaatggttg aacatacaga gcagtttatg
    16381 cctaaagata aaccaagata tttaatgggt gtaggatctc cagatgcgtt aatcgaatgt
    16441 agtattcgcg gcatggatat gtttgattgt gtcttaccga cacgtattgc cagaaatggt
    16501 acttgtatga catcgcaagg tcgtttagtt attaaaaatg caaaatttgc agatgattta
    16561 agaccgttag atgagaattg tgactgttat acatgtcaaa actattcaag agcgtatata
    16621 cgtcatttaa tcaaggcaga ggaaactttt ggtattcgtc ttactactat tcataattta
    16681 cattttctgc taaaattaat ggaagatata agacaagcca ttcgagaaga tcgtctttta
    16741 gatttcaaag aagaattctt cgagcaatat ggattaaatg ttgagaaccc aaaaaacttt
    16801 taagcaagag gagcgtataa aatgcaattt tcattactaa tatatatagt cgtaattttt
    16861 gcggttatgt atttcttgat gatcagacca caacaaaaac gtgcgaaaca gcatcgtgag
    16921 ttgattaata acattcaatc tggtcaaaga attacaacta ttggtggtat taaaggtact
    16981 gttaaagcag tagatgaaac aactgttgtt attacagtta atggtcatgg tactgaatta
    17041 actttcgaaa aacctgctat taaacaagtt gacccttcat aatgagaagt ggtattaaaa
    17101 aggatgaaac gaatagcgtt tcatcctttt taagttataa gtagtatgta gaatagctta
    17161 tttttattcc aaaaaaagaa attgtgatac ataataattg ttaaatttaa attaaatata
    17221 tctatgacta tttttactcc ctgctctagt atgttaatat gtataaggct aataaatttt
    17281 gtaagaaaga tttaacttgc gttataaaat agtattcaag tattctaaat acatacgtaa
    17341 ataatgaggt gttcatgtga agaaaagtag tagaataatt gcgttcttgc ttttagtcgt
    17401 attgttgttt gccggaatgg ctgcaactta taaaagtgtt ataaagaatg tcaatttagg
    17461 acttgatttg caaggtggat ttgaggtgct ttatcaagtc gatcctttaa ataaaggcga
    17521 caaaatagat aaaaaagcct tacaatcaac agcgcaaaca ttagaaaatc gtgttaacgt
    17581 tttaggtgtt tcggaaccta aaattcaagt tgaagaacct aatagaatta gagtacaact
    17641 tgctggtgta acagaccaga atgaagcacg taaaatttta tcttctcaag ctaatttaac
    17701 aattcgcgat gcagaagata aagtaaaatt aagcggttct gatattaaac agggatctgc
    17761 caaacaagaa tttaaacagg aaacaaacca gcctacagtt acttttaaag taaaagacaa
    17821 aaacaaattt aaaaaagtaa cggaagaaat ctctaagaaa cgtgataatg tcatggttgt
    17881 ttggttagat ttcaaaaaag gtgacagcta taaaaaagaa gcacaaaaga aaaaccctaa
    17941 atttatttct gcagcaagtg ttgatcaacc aatcaattct gatagcgtag aaatctcggg
    18001 tggatttaaa ggacaagaag gcgttaaaaa agcaaaacaa atcgccgaat tgttaaatgc
    18061 aggttcttta ccagttgact tgaaagaaat ttattcaaac tcagtaggtg cccaatttgg
    18121 tcaagatgca ttagataaaa ctgtgtttgc atcgtttatt ggtgtagcat taatttattt
    18181 attcatgctt ggattctacc gattacctgg tttagtagcg attattgcat tgacaactta
    18241 tatctatcta acgttagtag catttaattt catttccggg gttctaactt taccaggatt
    18301 agcggcgttg gtattaggtg taggtatggc tgtagatgcc aatattatca tgtatgagcg
    18361 tattaaggat gaacttcgaa taggtagaac gataaagcaa gccttttcta aagcaaacaa
    18421 aagttcattc ctaacaattt ttgattctaa cttaacaaca gttatcgccg cagcagtatt
    18481 attcttcttc ggtgaaagtt cagttaaagg tttcgcgaca atgttattat taggtattct
    18541 aatgatcttt gttacagccg tgttcttatc aagattctta ttatcattac ttgtttcatc
    18601 aaatatattc aaaaatcaat tttggttatt tggtgttaaa aagaataaac gacatgatat
    18661 taatgaaggt gtagatgttc atgaccttaa aacttcattc gagaaatgga attttgttaa
    18721 attagctaag ccattaattg gagtaagtat tttaattgta gttgtcggtt tagttattct
    18781 ttatatcttc aagttaaact taggaattga tttctcaagt ggtacacgcg tagatttcca
    18841 atctaaacaa gcaattactc aacaaaaggt agagcaagtt gtaaaagata gtggacttaa
    18901 agcggatcaa attcagatca atggtaaaga caataaagta gcaactgttc aatttaaaga
    18961 cgatttaacg cgtgctcaag ataataaatt gagtgataat attaaatcga aattcggtga
    19021 cacacctcaa attaacactg tatcaccaat tattggacag gaattagcaa aaaatgcaat
    19081 gcttgcatta atctatgcat cgataggtat catcatctat gtatcattac gatttgaatg
    19141 gcgcatgggt ctttcatctg tattggcatt attacatgat gtattcatta tagtagcgat
    19201 tttcagttta tttagaattg aagtagattt aacatttatc gccgctgtat taacaattgt
    19261 cggttattca attaatgata caatcgtaac gtttgaccgt gtacgtgaaa acttacaaaa
    19321 ggttaaagtg attacgacaa cagaacaaat tgatgatatc gttaatagat caattagaca
    19381 gacaatgaca cgttcaatta atacagtatt aacagttatt gtagtagtag ttgctatact
    19441 attcttcggt gctcctacga tattcaactt tactttagca ttatttatcg gattgatttc
    19501 tggtgtattc tcttcaatct tcattgccgt tccgctatgg ggaataatga aaaaacgtca
    19561 gttgaaaaaa tcgccgaaac acaaattagt tgtatataaa gaaaagaaat cgaacgatga
    19621 aaagatttta gtttaaaatg aattaagcgg tatgtgaaac aataaagagt tcacataccg
    19681 ctttttactt tatttaaatt taacaaaata tatataaata agttcccgca ttcattttta
    19741 aaaaatggta taattaaagt cttgattatt tagataagtg ctttttattt aaagtaattt
    19801 tagatataat gtcttttgga aatgaggcgt aaatatatat gattaaaccg aagtataagt
    19861 ggaaattaac taaaccagct gaatacataa gtgatgaatt aacatcaaaa ttaaagttaa
    19921 cgccaatcgt aaaaaaaata ttagaaagta aatcgattat tgatgaacaa gcaatagaat
    19981 ccatcatttc agatacagat atcaatcatg atgcactgca attgagcgat atgactaaga
    20041 ctattgaaag aattaaaaga gcgatagcga atgatgagaa aatcctagtt tatggtgatt
    20101 acgatgccga cggtgttacc gcaactacaa ttttagtgat aacattacaa cttttaggtg
    20161 cacaagtagg atggcatatt cctaaccgct ttaccgaagg atatggacca aacgaactag
    20221 catttagaaa tgctcatgat gaaggcataa cattaatcat aactgttgat aatggcatac
    20281 aaggtcataa tgaaattaaa atggttcaag atttaggagt tgatgtcatt gtaactgatc
    20341 atcatgaaat tggtagtaca ttaccagaag catatgcaat cgtacatcca atgcatccat
    20401 cgtttaatta tccgttccaa caattgtgtg gtgcaggtgt tgcatacaaa ttagcacaag
    20461 cattgattga aaatgtacct gattatttta aagcattagt tgcgataggt accattgcgg
    20521 atttagtatc cttaactgat gaaaatcgat cattagtgaa acaaggatta aaagtcttaa
    20581 atgatcaatg tccgacctct gttaaagcat tgctaaaaga agcgggatac aatgacaata
    20641 ttgatgaaga aacaattggt tttattattg gacctagact taatgctgta ggtcgtttag
    20701 atgacgcatc attagcttgc gaattgttga tgactgacgt cgaagaagaa gctgcatttt
    20761 tagcagaaca agttgaacat tttaaccgtg aaagaaaaga tattgtagca actatcacag
    20821 aagaagcaat ggctatggca gaaacgaaag ttaaaaaggg agatttattt ttacttttag
    20881 caaaagaaaa ttggcatgaa ggtgtcttag gtattgttgc ttcgaaaatt gtcgaaacat
    20941 ttgctttgcc aacattaatt ttaaatattg atagggagca aaatcatgca aaaggctccg
    21001 ccagatcaat tgatcaagtg tcgatgtttg aaatactatc agcacatcaa gagttaatcg
    21061 ctaaatttgg cggccatcat atggctgcag gtatgacgat ggatattgaa aatatagaat
    21121 cgttagctga aggtttaaat aaatggatga aagaattatc taaaacaact tcgttagatc
    21181 ccgtaaagca agtagatgta ttattaacag aaaatgatat aacgattaaa aatattcgtg
    21241 atatgaatcg tttaagaccc ttcggaacag atttttctag acctattttt gaaatggatg
    21301 atttatcagt ttcttcagtt aaggcgatag gtcaacaaaa aaatcatcta aaattgacac
    21361 ttggtgaaag taatatcgcc gcgctatttt ggcaaaatgg acacttagaa cctgagttac
    21421 aagatgaaca gccaattaat atattaggat ctgttcaaat caacgaatgg aatggtaatc
    21481 aatcaccgca aataattatt caagatattg cgatgaatga acagcaaata ttagattata
    21541 gaagtaagcg aaaaagttta ccttttacag aaaatgatga aaatattgtc gtgcttattc
    21601 atcctaaaag tgataaagta aatgcgaatg aatattatta tggtgaagaa attaaacaac
    21661 aaactgataa agtagtatta agagatttac caacgtcaat ggaagacttg tctaattcct
    21721 tgcaacaact gcaattttct caactttata tagttttgca acataatcat tcgatttact
    21781 tcga
//
LOCUS       DANJFZ010000037        21775 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.037, whole genome shotgun sequence.
ACCESSION   DANJFZ010000037 DANJFZ010000000
VERSION     DANJFZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.037"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(10..336)
                     /locus_tag="PWN45_001205"
                     /pseudo
     CDS             complement(10..336)
                     /locus_tag="PWN45_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            351..437
                     /locus_tag="PWN45_001206"
                     /pseudo
     CDS             351..437
                     /locus_tag="PWN45_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            439..936
                     /locus_tag="PWN45_001207"
     CDS             439..936
                     /locus_tag="PWN45_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0622983.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(996..1496)
                     /locus_tag="PWN45_001208"
                     /pseudo
     CDS             complement(996..1496)
                     /locus_tag="PWN45_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1544..1735)
                     /gene="vraH"
                     /locus_tag="PWN45_001209"
     CDS             complement(1544..1735)
                     /gene="vraH"
                     /locus_tag="PWN45_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDL0622984.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(1828..3708)
                     /gene="vraE"
                     /locus_tag="PWN45_001210"
     CDS             complement(1828..3708)
                     /gene="vraE"
                     /locus_tag="PWN45_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDL0622985.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(3698..4456)
                     /gene="vraD"
                     /locus_tag="PWN45_001211"
     CDS             complement(3698..4456)
                     /gene="vraD"
                     /locus_tag="PWN45_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDL0622986.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            4739..5251
                     /locus_tag="PWN45_001212"
     CDS             4739..5251
                     /locus_tag="PWN45_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDL0622987.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(5385..6179)
                     /locus_tag="PWN45_001213"
     CDS             complement(5385..6179)
                     /locus_tag="PWN45_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDL0622988.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(6422..7438)
                     /locus_tag="PWN45_001214"
     CDS             complement(6422..7438)
                     /locus_tag="PWN45_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDL0622989.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(7602..8177)
                     /locus_tag="PWN45_001215"
     CDS             complement(7602..8177)
                     /locus_tag="PWN45_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0622990.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            8402..9310
                     /gene="rarD"
                     /locus_tag="PWN45_001216"
     CDS             8402..9310
                     /gene="rarD"
                     /locus_tag="PWN45_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL0622991.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(9723..11141)
                     /locus_tag="PWN45_001217"
     CDS             complement(9723..11141)
                     /locus_tag="PWN45_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL0622992.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(11379..11843)
                     /gene="bstA"
                     /locus_tag="PWN45_001218"
     CDS             complement(11379..11843)
                     /gene="bstA"
                     /locus_tag="PWN45_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDL0622993.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            12010..12348
                     /locus_tag="PWN45_001219"
     CDS             12010..12348
                     /locus_tag="PWN45_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0622994.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            12338..13105
                     /locus_tag="PWN45_001220"
     CDS             12338..13105
                     /locus_tag="PWN45_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0622995.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(13224..13862)
                     /gene="pcp"
                     /locus_tag="PWN45_001221"
     CDS             complement(13224..13862)
                     /gene="pcp"
                     /locus_tag="PWN45_001221"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL0622996.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(14014..14970)
                     /locus_tag="PWN45_001222"
     CDS             complement(14014..14970)
                     /locus_tag="PWN45_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL0622997.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            15214..16185
                     /locus_tag="PWN45_001223"
     CDS             15214..16185
                     /locus_tag="PWN45_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL0622998.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(16334..16849)
                     /locus_tag="PWN45_001224"
     CDS             complement(16334..16849)
                     /locus_tag="PWN45_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDL0622999.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            17229..18077
                     /locus_tag="PWN45_001225"
     CDS             17229..18077
                     /locus_tag="PWN45_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDL0623000.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            18106..18660
                     /locus_tag="PWN45_001226"
     CDS             18106..18660
                     /locus_tag="PWN45_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDL0623001.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            18713..20425
                     /locus_tag="PWN45_001227"
     CDS             18713..20425
                     /locus_tag="PWN45_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623002.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            20422..21255
                     /locus_tag="PWN45_001228"
     CDS             20422..21255
                     /locus_tag="PWN45_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0623003.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            21302..>21775
                     /locus_tag="PWN45_001229"
     CDS             21302..>21775
                     /locus_tag="PWN45_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623004.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQ"
BASE COUNT         7415 a         3350 c         3749 g         7261 t
ORIGIN      
        1 tataaatctt tagattgtca aattaagctg gacaagttta tcatcttcga tgaaactcat
       61 aaatacttcc aatggtgttc tataacctaa agattttcta ggatgatgat tgattttttg
      121 agcaacactg atgatatgat catccgttac taaattgaaa tctagttctt tatctaatcc
      181 actgcgtcta attattccgt tagaatgttc atttaaacct cgctgtgatg gcgtgcctgg
      241 atcagcaaaa aatatagata tatcgtgaca gttacatatt gttttccagt ttgagaactc
      301 cttaccacaa tcaaaagtaa ttgatttaaa tatatgagaa gataaaatga aaaggtgcaa
      361 gcagtattaa gaatttaggt agcaagaaaa caagaagtgg tacatacgat gctaacctaa
      421 aaagaattgg agattaaaat gattattaac cttgaaacac aacaaataca ttttgattta
      481 gaacatgaaa ttccctattt tcaagcacct gagaaaaata ggatacgctt agatattgat
      541 gttctcaata aaaagcaaat ttctaatatt attaatgtta tatttaataa ccaatcaaag
      601 acgaaatgta catttttgtc tgaatactta tatcctgtta aatttagaga aaaaacaaga
      661 attggtcgtt tttttaatat tactaactgg tacgaagaaa tccattctac cgatgagaag
      721 tatgttattg caactattaa aaatataaaa aacaaagact taattgatta ttctttatat
      781 attaaaaaag gtttagtgga gccatttatt gtattctata atcaagaaaa tatattctac
      841 attagcaatg ctgttataga tatcatcagt gtatccaaag atactcttga ttatataaag
      901 tcagaattca atgatcactg tgcttcgcca tactgatttt atagcacaga gaaaagccgt
      961 agtttcgaag agaccaacgg cttttttata aatctttaga ttgtcaaatt aagctggaca
     1021 agtttatcat cttcgatgaa actcataaat acttccaatg gtgttctata acctaaagat
     1081 tttctaggac gatgattgat tttttgagca acactgatga tatgatcgtc tgttactaaa
     1141 ttgaaatcta gttctttatc taatccactg cgtctaagta ttccgttaga atgttcattt
     1201 aaacctcgct gtgatggcgt gcctggatca gcaaaaaata tagatatatc gtgacagtta
     1261 cataatgttt tccagtttga gaactcctta ccacaatcaa aagtaattga tttaaatata
     1321 tgagaaggta aagatgaaaa cattgagtgt agagcgtttt ctatatcagt tgccttacga
     1381 cctaagggtc gtaaggtgat aatcatcttg gatactcttt ctaccaaagt aatgattgca
     1441 ctaccatgac gacgaccaac aatagtatct ccttcaagat gtccaaactc cttatgtttt
     1501 ctttggtagg aaatacatat tgagtttagc acataaggct tatttattta cctttttctt
     1561 cacgacgttt taaataatag gcgccaccta ataaaccagc tggaatgcct atcattggtg
     1621 ttgtgaatga gcttaataca ataacaagta ttgttaaagc aatgacgtta taccaagtta
     1681 cagtcaaatt tttcaaatcc tcatatgatt gttttactaa ttctctaaat ttcatgattc
     1741 aatctctcct tttcttaaat caattaatag ttatactgca ttgcttaaaa tcaaaatatc
     1801 taatgaagca tcttttaatc gtaagtgtta aatggttttc ttaatcaatt tgtttgaatg
     1861 tacaaaagca attaaagcaa aagtaatgta aatgagggtg tatacaacaa ttactacaat
     1921 aaccggcatg aaggaaatat tacccatcaa tttcataaat gctattgcgg cgaatactgc
     1981 atgtaatata gcaatcaata atggtaaacc gaagttgaaa gtaattttta ataataatcc
     2041 cttaagcata tctgtatgcg taaagcctat gcgttttaat attctgaagt tacttagttc
     2101 atcctcagtt tcatccattt gtttaatata aataatacat ccagctgcta ctaaaaatgc
     2161 taatcctaaa aatgatgtaa caaatattag aataccgtta gtagcatcga cctctttttt
     2221 catttcatca tacgtgatga ctttgtctcc aaactgtttt gcaattgctt gagctttttc
     2281 cttttgtgat gtttgtttaa tatcatatcc ataaaaagta tgaacgttat tttgtgtttt
     2341 caactgctga tacttttcag gacttacttc gatgacaggt gagttgaagc ttagatttaa
     2401 aggataaacc ttacctttgt cttcttgtgt aacacggaaa gtttcattct tagtaccttt
     2461 tactactaaa tctttgttta aatggatatt aataatgtta ggcagcgatt ttgtatttgt
     2521 aatgatggca ttgttgcctg ttaacttgtt atttgcactt aaaatagaat tcgtgcgacc
     2581 tgaatcacta ccattttcca aagtaataac ctgatcatta acattatcta cagtaatagt
     2641 ttcgtaagca tttttagaaa atgtaatttg ttgttggctt agtttagttt caaattgttt
     2701 agcatcttga gtagcgacca cgttaaattc atttggtgcc atagatgtaa gggtttgatc
     2761 tgtatttgat ttagataatg ccgcaaaaca caatacagtt actgtaactg cagaaataat
     2821 tgcaatgata gttaaagaca tggcattttt cttcattctg tacataatag acgatgtgaa
     2881 tacaacatcg gtaatagata cgcgtccatt ttttgatttt ttcaatgttt taaaaataag
     2941 tgacacggaa cttctgaaga ataaataggc gcctacaacc gttaaaaata aaatgataaa
     3001 cggtgatgtc atagccatag ttagtgcttt gaacgtacca aacatttctg tcgccatata
     3061 atagcctagt gcaatcatag caatacctaa tacgcctgaa ataacctctg cagtcgttac
     3121 tttagcagtt gtggcatcag ttttaattga atctttcatc attgataaga tactacgtct
     3181 ttttagaaat aaagcacttt taaataaaat cagtacatac gcaataatta gcatgaaaat
     3241 agttaaaaca agggccatag gttcgaaatg tatcgataag ttaatcgata acgacatcaa
     3301 tttagatact atggaaagca ataattgtgc acctgcaatg ccacataata caccgacaac
     3361 acctgtgatt aaaaatacga tcatttgttc aagtgctaac attttcaaaa tgttttgtcg
     3421 tgttaaacca atcaactgaa atagcgcaaa ttcacgtgta cggcgtttta cgaataaatg
     3481 attggcatac attaaaaaga tgacaataat gataaataaa aatattgatc cgactaaagc
     3541 acctttctta atgatggcca tcgagtcgtc attatttaca cctttagtaa actgtaaggt
     3601 tgtaaaactg aaatataaga cgatgctaaa aaataatgaa aataaataca ttgcataatg
     3661 ttttaagttt tgtcgtaagt ttttgaaaac gatatggtta aatgtcattt gagacaccac
     3721 ctaatactga ttgaagatgt acaatgtctt cataaaaggc ctgtttagaa cgtccttcct
     3781 gataaagttg tgtatgaatt tggccatctt ttagcatgat gacacgttct gcaaaacttg
     3841 ctgcaaccgg atcatgtgta accatgacaa tagttgtatc aaacgattta ttcatttctt
     3901 ccaaacgttg taataggtca tttgcacttt tcgagtcgag tgcgcctgtt ggctcatctg
     3961 caaatatgat ttgtggtttg tgaacaaatg ctctcgctgc tgcagttctt tgttgttgac
     4021 caccagataa ttcgctaggg tatttatttc ctaggtcata aatacctaat gctgtcgtga
     4081 tcgctttata attttcttcc attgttgcct tcgacatttt ttgaacagat aaaggtaaca
     4141 taatgttttc tttaacggtt aatgtcggca gaatactgta atcttggaag atgaaaccta
     4201 atgattcttt gcggaatttg gcaagtgctt tttgattaag tttattaagc tcttgtccgt
     4261 tagcaatcac gctaccgcta gaaatttggt caattgaact tagtacattt aataaggttg
     4321 tcttacctga tccagaaggc cccataatcg caacgaattc gcctttttgt atgtcaaagt
     4381 taatatcttt aagtgcttga aatgtgtgct ttttaccgta tgtttttgaa acatgttgca
     4441 ctgataatat cgtcatagtc tcactccttt tgtatttaat ttcattttaa ataatgtttg
     4501 gagtagtagc ctttatctaa acttacaatt caatgaatga accttacaga gttgaaagct
     4561 atcgctactt agtagatttt tgagtgagga tacagattca tcgtacatat tagacaaaag
     4621 caatggtgct ttctaagtga tgatgtttgt gtaaattgag aaaagggaat ttaattattg
     4681 tataataaat tttttgtaaa aattaaaaga gggttttatt tgaaaggaat tgattgttat
     4741 ggaaaaagga aatcaaggta ttaaatggtc tagtttaata atgggtgtat tattattaat
     4801 gttggcagtc gttattttta catttccaat tgaaaatttt tatgctatta cctggttgat
     4861 tggactgttt gtattaatta acggtgtgat tcaaatcgtt taccgtagaa aagcaaaagc
     4921 tttagtaggt ggtaaccaaa attggattct gtttatgggg attgtagata ttctatttgg
     4981 tctattagtt atttttaatg ttggcgcaag ttcagcattc tttatttata tgtttgcttt
     5041 ttggtttatt tttagttcta tctctggatt atttacgttt tcgggtagtg gtagcttaaa
     5101 actaatttca gtgattttta atttattagg tattgttttc ggtgtcattt tattatttaa
     5161 tccattaatg ggtatcgtct ttatttcgac gatgattgct attgcatttg tattcgtagg
     5221 tgtcatttat gttgtagatg cacttgctta agtaaaatga agcggttcaa aagaagggtg
     5281 tgacatgaag tttgtgtcat atcctttttg ttgtgtttat gaagcataaa aaaggggcgc
     5341 tacctacaat aagtaagata cgcccatatt tttatatttt actattattg tttttcaata
     5401 cgattaatag ttacatttag tccaaaatat ttttctaaaa aatgtttata gttatcttta
     5461 gtgacatcaa atttttctga gctaccattc cttgttaaag ttaaatgatt ttcagacatt
     5521 gtagcacggc caaatgattg tggcattgta attaataaat gctgtacaaa tattgaatct
     5581 ggatgcgttt gattatattc gatgttgcta ttaaaatctt ctatttgttt aggtttgaat
     5641 tcagcttcat attttgtatg ccaatgatca ttttcgaatt tttgaacata gaaaatatcc
     5701 ttgtcttcgt tgttaaaaat agcgcggaat gtaccactga tatcagtaat tggttgtgta
     5761 tgctcagatg aagtaatagg aatggcatgt agaggtaagt ctccaaagcc aacatcagtt
     5821 acatagaata catcatttat agaaacaaca agtgaagcat gtgaaccgtt cagactacga
     5881 ccgccaccgg gtgtgtgaat agtagctgac attaattcag gattaaatcc tttttgttgt
     5941 aaataggctt tgaaaaatgt atttaattca taacaaaaac caccacgttt atcatgaaca
     6001 attttattaa aaagtgcatc gatatttata gatatcggct tactattttg aacatcaata
     6061 ttttcaaaag gtacagttaa cataaaacgt gttgcataat aatttaatgc ttcaatactc
     6121 ggtcgattat aacgagatga atcaatttgt aaataattct ctaacttcgc aatattcata
     6181 agcatagcgc ctcctgtatt aaagattata attaaatttt aaacagaaat actgaaattt
     6241 taaattcgaa agcattgaat tttggataaa tacattttaa atagaaaaat acgctctcaa
     6301 aatgaagtca tctctaaaag aaacgattta aagatgacta ctgagagcgt agcataatgg
     6361 aagaagtgtg cagggtgtct aaaaatgcaa caatacaaag gtagttgcaa gacaagttgc
     6421 cttatctaga ccatttgtgt tctatgcgac caaacttcca aattaaactt gaaataagcc
     6481 aagtaattaa aaataatgca actaaaatat agcctaaata atcaaattcg atcgaaccaa
     6541 tgaatgccca aaacgcacca tgtaaatcta acttatcagc aagaatttgt agcaattcaa
     6601 tcatcccaat cactaatgct gccatgactg atatcgcagt aatcgttata ttgtaataga
     6661 ttttgcgaat aggattgaag aatgcccaat tataggcata cttcattaca acaccatcta
     6721 atgtatccaa taaactcata cctgatgcga ataaaattgg taaagataag attccgataa
     6781 atgaaatggc ttgttgtgat gcgcctgaag aaagagcgag taacgcaatt tcactagctg
     6841 tatcaaaacc aagtccaaat aaaaagccaa gtggcaatac gtgccaacta cgcgtgatta
     6901 atttgaaata aggtcctaca aatcgagaaa ccaatcctct agattcaagt aatgcatcga
     6961 cttcagcttc ttcaatgtgt tcacgacgta atttagcgaa caagttaatt aaagagatta
     7021 aaataattag attcaacaca ccgataagca ctaaaaagaa acctgaaact agtgtaccaa
     7081 tcgttccacc aatatcttgg aaatgcggta attcatcttt agcccatttt acagataccc
     7141 ctaaaaaaac agccattaaa aatacgacag atgaatgtcc aattgaaaaa tagaaaccca
     7201 caccagatgg atctttgcgt tgctgtaata atttgcgaac cgtattatct attgcagcaa
     7261 tgtgatctgc atcaaatgca tgacgcaaac ctaatgtata tgcaagaatc cccataccaa
     7321 ataagatatg atggtctttt ccagcaatcc ataaaaaact aaacccaata acgtgtaaca
     7381 aaatgacaat agctatgtat ggtaaccagc ttaagcgctc atttttaaaa acagtcaaaa
     7441 tatatccttc tttcataaca tgatatttaa tcatactgta tgttcaatgg gcactctagt
     7501 aataagtgtt catataacaa aaatgttatg ccaaattatt tgttatataa aaatatacat
     7561 gtaaccacaa aagatttttg cgatatatat aatttgataa attaaccaac aacaatgtaa
     7621 gatgtcactt tgcttaactt ggcatccttt ttatgatttt caaattcaaa aaaatgagca
     7681 aaatgaatct ctttaccagt ttttaatatt tcaataccat gcatggaacc taagcaccca
     7741 tgtgtgatgc tggaatggat attgagacta gcaacctgat tgtaatgatt agatagttct
     7801 tgaattaata tttgaggtcc gtatatgtca aagcggccag ggacagacca aataaattct
     7861 gttgtaacca gtgaacgtaa taattcaata tctaatgctg ctgtaacaac tataaaatct
     7921 atcatttgtt gacgtttagg cgcatgattg catgacacat ctcctgttaa cttaaaaggt
     7981 aatgatgact gaacttccgt tttaaaatgt agttggtgct gaaataaagc ttgttccaat
     8041 attgatatag ctttaggacc aacgccatgc atctccatta gagatgactt cgtatattgt
     8101 gatactgctt ctaatgtgta tataccttgt gaatttagcg cgcgtgttgc aggctttcca
     8161 attttaggta aagtcatttg tatccatcct ttatgttgta tggtagtctt tctggcaata
     8221 taccctgatt tgtacagaat tatttataaa attaatattg tttagtggtt ggtgaataaa
     8281 tgtgggaaat ataaacaaaa tgatatcttt atcgaaatat tattagtaaa atcgttatat
     8341 tagcataacc aagtgagcgt atgcagtaaa ttaaaaatta ggaaagtaag gaggtttgaa
     8401 ggtgaattca gagtataaaa aaggcatatt tttagcactc agtgcataca ttctgtgggg
     8461 aatactacct atatattggc agttcgttga tgcaataggc gcatttgaaa ttttagcctt
     8521 tcgtattata ttttcagcaa tattcatgat tttcatactc gcggttggac aaaaacaacg
     8581 caatgcattt caacgagata tgaatcaatt gttaggcaag cccattcagc tattagcgat
     8641 tgtcgtagca ggctatgtca ttacattaaa ttggggtaca tttatttggg ctgtaacgaa
     8701 cggtcacgtc ctacaaacaa gtttaggtta ttatataaat ccacttgtta gcattttgct
     8761 cgcacttatc tttttaaaag aaagattcaa taaatttgaa tggctagcca ttttattcgc
     8821 attcatcggt gtattatata tgacgctcaa gattggagaa ttcccaatcg tctctattat
     8881 attagcgtta tcctttggta catacggatt attgaaaaaa gtagtacata ttgatgccat
     8941 cagcagtatt acgattgaat gtattgttac cgcacctgct ggactaatat acgttattta
     9001 tttatggcag caacatcaga tgtcatttgg attgaacatg tcatcatttt ggttgttatt
     9061 ttctggtgct attacggcaa taccactaat cctattctca gccggggcaa aacgtattcc
     9121 actttcgcta ataggattta ttcaatacgt tggaccaaca ataatgtttg tactcggcat
     9181 atttgttttc aaagagcctt ttagtataga tcaattaatt acgtttatat ttatttggac
     9241 aggtattgtg ttatatagtc tttctcaata cattaaattg aagaaacatc cggtcgcaaa
     9301 aaccctataa aatacgagga aagataatta tacgtgtttt caatatcctt tgtgtcatga
     9361 tgaggtgaga ctgaggtatg tgtgcagtaa cagtgacaga cgtataaatt tgaaacatta
     9421 aatatgaaat aaggcacttt cgttgaattc gcaattaaaa tgaatttaaa cgaaggtgtt
     9481 ttttgtatga tgatttatga gggcatacgt aatgttaatc aatgaagcgg aatagtatag
     9541 aaatgtattt tatgtgatgt tacgtaatga cttaaatctt tatacaaatt ttcagtgaag
     9601 cacgcaatga tttaaaggat aaacgtattt taaaaaggga gtcttgattt agcaaaggtt
     9661 gattttaaat acaaattaaa aagcgcccaa atcaatgaga ttcgagcgct aatttaaata
     9721 ttttaaaata taccaattac tttcatccaa agtgatccta aaccaatcca gataataaag
     9781 tagacgaaac ctaatattaa gttcattgtc caccaacgtt tttgagtcac gtaacctgaa
     9841 gagaatagaa tcggcgctgg accactacta tagtgtgttg ttgaagctaa taggttaccg
     9901 aagaaaccta acattaatgc actgaataat ggtggtgcac cggctgcgat ggcaacgcct
     9961 agtaatgctg catacatcgc actgatatgt gctgtagaac ttgcaaataa gtaatgtgaa
    10021 tagaagtaga acaatattaa aatgaccagg actataggcc agcttaagcc accaagactt
    10081 gtagcaatgg atttacttaa ccaaggaata aatccaagct tgtttaattg gtcggccatt
    10141 aacactaata ctgagaacca tactaatgtg ttccaagcac ctgtttcgtt taaaatgtct
    10201 tgccatgtta agacgcctgt caataataac aatgctagcg caataaaggc cgttaaagtt
    10261 gcatcaatat gaatgaaact tccgacaatc catagtgtta acgcaacgac aaaaatacca
    10321 atcataaatt tttcagctaa agcgatttta cccatagtcg ctaattcatt ttcagcccaa
    10381 ctcttagcat ttggtgtttc tttaacagtt ggtggataaa ttttataaat aataaaaggt
    10441 acaacaatta aggaaacaag tccaggaact aaagcagcta gaaaccaatt catccatgta
    10501 atgtgaacat tagatgtgct agatgctaaa ttttgtgcaa gggggttacc ggccattgca
    10561 gttaaaaaca tagccgcagt aattaaatta ccttggaatt ctgtgaaaac aagaaatgca
    10621 cccattttgc gtgctgatcc gtctttcggt ttcgaaccaa atgattcaga aagtgatttg
    10681 ataattggga acatgattcc accagcacgc gcggtattac ttggtgtagc aggcgctaga
    10741 attaaatcta caccgacgat agaatatgct aatcctaatg tttttttacc aaataatttg
    10801 acgaaatgaa gtgcgatacg tctaccaaga cctgttttca caaatcctct cgaaatgaaa
    10861 aatgccatag caattaacca aatgctatta ttaccaaaac cagcgacagc cgttttcatg
    10921 tcaacaatgc caacgagtac catgattgta aatccaatta tagagacggc cccaattggc
    10981 atcggttgtg taatacaagc aatgattgtc gcgacgaata ttgcgaacat ataccatgct
    11041 gttggatcca cagcatccgg tttaaaaggt gtaagtgccc aaataaggag acctacaacg
    11101 atagggagta taaacttacg atatttaacc gtgttttcca tgttaaaacg tccttctttc
    11161 tatgttttat acatatttca atttaagaat aaagctaact acaaaagatg tacagtaata
    11221 attaaatata aaattaaatt aacgaaatca ttaatataat tatttttcga gaatcggtga
    11281 agaactggta tagatggtgt ttattaaatt caaaaaagtg accttttaaa caaataaaaa
    11341 acccgttaac gtgttgttaa cggttaataa gcacatattt attttaaatt ttttaatata
    11401 cgagacatct cttcaatttt accagcatga agtgggatgt gaatggcatt taatactaat
    11461 aactcttcta agttattaca accgacaatt ggctgtttca attcaattgc taaatcatct
    11521 tcagttagat ttcgtgcacg ttcaggtaaa gtttggagac cttctaaaat cctttcaata
    11581 ctcggtactt catccttcca ttcatctgga gacgaaccat ttccgaataa aggtctatag
    11641 atatttaaat caatattctc tttaccagca acagctaagg ccgattcaaa aattgttaat
    11701 acatgaccaa attgccaatg aatggtatta gaaaaaggct tatgataatc atcaagtacc
    11761 ttttcaactt tccaagcatc atacacattt actaaatatc ctaaccccat atcaatgaca
    11821 tcaaatactg tttttgtagt cattattaat tcctccccca taaataaaat atacttccgt
    11881 aagtattacc ctattaacaa agttgttagt catttaagga atgtgtacaa caactacctt
    11941 acaataaaag atagttgacg aaaagttctc aatgtactat cttattatgt aagatagtag
    12001 gtgattaaaa tgtcagtgtc agatcagatt atgaaaggtc tcttagatgg ggcgatatta
    12061 ggtctcattg cccaaggcga aacgtatgga tatgaaatta tgacgaaact taagaatcaa
    12121 gagtttccag agataagtga gggtagtatt tatccagttt taatgcgttt aaacaaaaaa
    12181 ggatacgttg atacgacaat aaaaaaatct agtaatggcg gcccgaggcg gaaatactat
    12241 acgattaccg attcaggttt aaaggaacta aaagtattta aatcgaaatg gcaagttttg
    12301 aataaaggga tgattaactt gtttggaggg gaaaacgatg ttaagtaaac aatctcaaga
    12361 ttttttagaa aaattacgtg ttgaattgtt atttagaggt aagactgaag cagaagtaga
    12421 agagttattg gaagagctcg aagatcattt aactatggct gaaaaaaata acgaagatac
    12481 aagttcaatt attaatacac cgattaagtc aatggctgat caattatcac ctgagataag
    12541 tcttactacc ggtttgtata aatatattac tttgtacttc gcttttattt tagcagtcat
    12601 tataattcct aggtttttag atttgggtac atttgatgtg acaattgcat ttttacttta
    12661 tatagctagt atcgttattt taggtgttgt cattggtatg ctgattctac gaaatacatt
    12721 agtccgattt ggtgaccaaa aaataacgta tgttataaat gtgacttatg gaattatcgt
    12781 atttttatgg atgattttag gtggcttgat tattaaaaaa tatcctatct atagtttctt
    12841 tgagttaagt gacaaacaga gtttcatcgt tgggttagta cttttaattg cgctcattgg
    12901 aacttgtatt ttaattaaac agaaaattta tgcgctcatc gtgttggtaa tttcattgcc
    12961 aagtttgata gcaaggatag cagctatctt tagtaaagaa ccagacactt ttaatttagt
    13021 ttcagtaagt atactcatca ttctaaatat agcatttatt atttatacat ttgtaaattt
    13081 taggaagtta aaaaaagaag actaaacaat gtcgtttagc taaaatacat cattatagta
    13141 aaactgtata ccaatcatcc aaaagcaccc taaagttaga cttaaactaa caaattaggg
    13201 tgcttttgtt tgtgcactta taactattcc gttgtgccca gggctaggtg taaatcctca
    13261 tcattagaaa tagcttcaat ggctgcagtc aaacctgcaa ctatcttttc taatggcata
    13321 gatggtgtat cgggtttacc aatgacctgc tctggtatgt atggcacatg aataaatccg
    13381 aatcgtaggt gagggtaatg cttatcttgt aaataaccta agtgataaag tgtgtgatta
    13441 caaacaaatg tacctgcgct atttgaaagt gctccaggaa gtccttgatt aataatactt
    13501 tgagtcattg ctttaactgg taaatttgaa aaataagctg gcgcaccgtc taagtgaatg
    13561 gcttgatcaa taggttgaaa atcatcatta tctggaatac gtgcatcatc aatattaatg
    13621 gcgacacgtt ctggggtaat ggcatttcta ccaccagctt gtcctatagc tagtacaaca
    13681 tcataatgat tagatgccaa cgttttattt ataatattat ctactttctt aaaagaggtt
    13741 ggtagtttta atttatcgat tgtatgtgtg ccaataatat cttctagttg agtcacagct
    13801 tcccatgagg gattgatatt ttgattgtca aaaggcgcaa accctgttac taaaatgtgc
    13861 attttaagac accccgcagt attaaaattt aatttagtat aacacgcgca taattaaaaa
    13921 catatcaggt tatgtatgcg cataaaaaac agccttgaaa aacctttata aatcggattt
    13981 tcaaagcgtg ttttgaaagg tatttaatta aaactaagca tgttgatgta aatcatcaaa
    14041 gtttgttaaa cgttgttgcc actcattatc actaatatta tttgcttgaa cataacgatt
    14101 acgctcatgt ttagcacatt cataagagca tgcacctaaa tatttagttt cgttttcttc
    14161 agaaactaat atttgtttat tacattctgg gttagcgcaa ttaatataac gttcacatgg
    14221 tttgccatca aaccaatcct taccaataat tgttttttca acttggttga tatcaacact
    14281 gatacggtca tcaaatacgt acattttacc gtcccaatat tcaccttttg tttcaggatc
    14341 tttaccatat gtagcaatac cgccatgaag ttgagctaca tcttcgaaac cttcttttaa
    14401 aagccatcca gaaaattttt cgcatcgaat gccaccagta cagtacgtaa ccactttttt
    14461 atctgcaaat aacgctttat tctctttaat ccagtcaggc aaatctctaa aacgtgtgat
    14521 atttggacga attgcacctc ggaaatgacc taaatcaaat tcataatcat tacgtgcatc
    14581 aataatgact gtgtcatcat cttcaagagc ttttctaaat tctacaggtg ataaatattg
    14641 gccagttgtg tgtcttggat cgacgtcatc ttctaaatct aaagcaacga tttcttttcg
    14701 aggacgtaca tgcattttct taaaagcatg tccttcagct tcatcaattt taaacaccat
    14761 atctttgaat cgttcatcgg catgcatatg tgccatatat tgttcggttt cttcttttgt
    14821 accagataat gtgccgttaa taccttctgt agaaacaaga attctacctt ttaaatggtg
    14881 tgctttacaa aaggctaagt gatcctgagc aaactgttca gggtcatcaa tcgtcatata
    14941 tttataatat aaaagaactt gatagttcat aggttaatct ccttctataa acatgttata
    15001 agcatgttaa tttttaaaat aaagcgtcaa tacgcatatt tgtctacgtc catcatttta
    15061 acagagttgt attgtcattc aatatttcta gcttgaaatc agccactaga ccgataaaat
    15121 agatagatga aaccgttatc attttatttg atagttatct tgtttaaaat aaaattgata
    15181 gttaaaacga atgaaatgaa aggaatgatc atgatgtcac aacaagattt acctacatta
    15241 ttttatagcg ggaagtccaa tagtgctgtt ccaattatat ctgaaagtga attacaaaca
    15301 attacagctg aaccatggct tgaaatttcc aaaaaaggat tgcaactaga aggattgaac
    15361 tttgatcggc agggacaact ctttttattg gatgtattcg aaggcaatat tttcaaaatc
    15421 aatcctgaaa cgaaggaaat caaacgacct tttgtaagtc acaaagcgaa tcctgcagca
    15481 atcaaaatac ataaagatgg ccgattattc gtttgttatt taggagattt taaatctaca
    15541 ggaggcattt ttgcagctac agaaaatggt gacaacttac aagatattat tgaagatctt
    15601 tcaacagcat attgtattga tgacatggta tttgattcta aaggtggatt ttattttaca
    15661 gattttagag gatactctac caatccacta ggaggcgttt attatgtttc gccggacttt
    15721 agaacagtga cgcctatcat tcaaaatatt agcgtagcaa atggtattgc tttaagtaca
    15781 gatgaaaaag tactatgggt aacagaaaca acagccaatc gattacatcg cattgcactt
    15841 gaagatgatg gtgtgacgat acaaccattt ggagctacca taccgtacta ttttacaggt
    15901 catgaaggac cagactcatg ttgtattgat agtgacgata atttatacgt agcaatgtat
    15961 ggtcaaggtc gagtgttagt ttttaataaa aggggttatc caataggaca aatattgata
    16021 ccaggccgag atgaagggca tatgttacgt tctactcatc cgcaatttat acctggaaca
    16081 aatcaactca tcatttgttc caatgatata gaaatgggcg gaggatctat gctttataca
    16141 gttaatggct ttgcaaaagg tcatcaaagt tttcagtttc aataagacct acctgaatga
    16201 aaggcgtcta aaattagggt gaacagttag tgcttgttac acatgagtta aacgaaaaat
    16261 gccaactata cctagggatg atatagttgg catttcgtat atatattaaa acaatgagga
    16321 ttgtgtaatg agattattct gagattgaga attcagctga tgcttcgaat ttaacatctt
    16381 tgcctagcat tacgccacca gtttcaagtg cttggttaaa gttaatgcca tatttttctc
    16441 tattgattgt accagtaaca ataatacctg ttacttgaga accatccata ggattcttac
    16501 ttactccgtt gaattcaaca tcgaatgttt cttcgttagt gatgccttta attgttaaat
    16561 caccaacaac tttgctttca gatactgatt ttgtcacaaa tgtaatttta tcaaattcgt
    16621 cagtaccaaa gaaatcacca gattttaagt ggttatcacg tgcttcgttt ttagtgttaa
    16681 ttgagcttgg aataattgtt gcagtagctt tcaaagtact gaagtcatta atatctcctt
    16741 caacagctac atcaaattga tcaaatgaac ctttcacttt agaaaccatt aaatgtttaa
    16801 tttggaattc taaactactg tgtgcaccat caaaagtaaa gttagtcata atataaaacc
    16861 tccatatttt tatctcttag atatattttt tatacttgat atacatacaa taccacctaa
    16921 aaaacaaaga tgcaagcatt ttatttcgaa ttcgaatatt ttctgtgatg tatttaaaga
    16981 atgacctata aaacaccaca ctattgttat accctagtca attaattcaa acataaagaa
    17041 gtgattacaa gtccgtgaaa tttagttaat gagataaatc tgactattat tatcattttt
    17101 atagtcacta ctattgactt tgcatcaagt gactcgtact attttaatat aattctgaaa
    17161 attcaaaata tagaacatta aatatgtaga taatattttt ttgaagtttt aaaaaaagga
    17221 ggcattttat gggaaattat ttggtattgc aatcagattt tggacttagt gatggcgctg
    17281 taagtgcaat gtatggagtt gcatatactg taagtgatga tatacgtgtt gaaaacttaa
    17341 cgcatgatat accaccgtat gacatttggg tagcgtcata tcgtttatat caaactgtaa
    17401 aatactggcc taaaggcaca gtttttgtat cagtggtaga cccgggtgta ggtagtgata
    17461 ggcggagcat tgcttgctta acatattcag gtcattacat tattacacct gataatggtt
    17521 ctttaagtca tattaaacat tacgaaggta ttaaaaaagt tatcgaaatt gatgaagtga
    17581 aaagccgatt gcctcattcg gaagaaagtc atacttttca tggtagagat gtttatgcat
    17641 acaatggtgc gagattagct gcaaataaga tagcgtttga acgtctcggt caggcaatta
    17701 atgttgatag tattgaggca ttagaaataa gagaagtgac aaaaaatgag gattctgtta
    17761 caggaagcat tgatatttta gatatcagat ttgggtcatt atggacaaat attccattgg
    17821 cattcttaaa atccttggaa attgttcatg gtaataattt ggtggtaaca atttatcatc
    17881 aagataagaa agtctatcaa aatattatta aatttgcgcg atcgtttgca gatgttaatg
    17941 tgggtgaacc tcttgtgtat gttaactcgc tagtaaatat tggtgtagca gtcaatcaag
    18001 actcattttc agatttatat catattggta ctggaaatga ttggacgatt catttatcta
    18061 aagcaccatc aatataatga attttttatg taaggagtaa tgaagatgaa aaaacaagat
    18121 atttcagtta aaactgttgt tgcaattggt ataggagcag cggtatttgt aattttaggg
    18181 cgttttgttg taataccaac aggttttcct aatacgaata tagaaacatc atatgcattt
    18241 ttagcattaa tatctgcaat atttggacct tttgctggtt taatgactgg attagttgga
    18301 catgctatta aagattttac gacatacggt agtgcctggt ggagttgggt tatttgctcg
    18361 ggaattatag gctgtttata tggctggatt ggactaaaat taaatctttc ttcaggccga
    18421 ttttctagga aatcgatggt ttattttaat atcggtcaaa ttattgcgaa tattatttgc
    18481 tgggcactta ttgcaccaac attagatatt ttgatttata acgaaccagc taacaaggtt
    18541 tatacacaag gtgttatctc tgcagtatta aatattattt cagttggcat tattgggaca
    18601 atattattaa aagcatatgc ttcatctcaa attaaaaaag gtagtttacg taaggaatag
    18661 ttattttgtt gaatcagata tgtaaatgaa tatagaaagg taatgatata tcatgactga
    18721 accaattatc tcgttcaaag actttagttt tcaatatcat agtcaagcaa cacctacatt
    18781 acagaatata aatgttgata tttatccagg agaaaaagta ttagtagttg gtgcttcggg
    18841 tagtggtaaa tcgacttttg caaattgcat aaacggatta attccattta aaactaaagg
    18901 taacataact ggggaactat atataaataa tcaagatgca accgttagtt gtttacatga
    18961 tagatctaat gttgttggta cagttttaaa agatacagat ggacagttca taggcttaac
    19021 agcagctgaa gatatggcct ttttattaga aaataattgt gttgaacaag atgatatgaa
    19081 gaaaaatgta agttattggg ctgaaaaagt tggcatgata gaacatttaa atcaccgacc
    19141 gcaagattta tctggaggtc aaaaacaacg cgtttcatta ggtggtatat taatccatcg
    19201 tacgcctatt ttaatattgg atgagccact ggccaattta gatcctgcga caggacatga
    19261 aacgctgaga ttgttaaaca atattcatga agaaacaaag tcaacgatga ttattgtcga
    19321 acatcgatta gaagaatcat tagatgatac gtttgataga gttcttcttt ttaaagatgg
    19381 aaaaatcatc gctaatacaa cgcctagtga tttgctaaaa tcatctaagc ttaaagaagc
    19441 aggcatacgt gaaccattat actgtacagc gcttaaatat gcagaagtag atgtcgaatc
    19501 cattgataac ttagcgaatt tacgtgacgt ttgtatgagt gaacatgtta aatttaaagt
    19561 gaaaaaatgg atagatgaaa catctgcaaa taatgataat aagtacaaat ctgaaccgct
    19621 attagaatta aatgaggtat gtgttcaata tagtgactat agtaatagtg tacttaataa
    19681 tgttcaatta aatgtttata gaagagaaat gcttagtata gttggtcaca atggtgccgg
    19741 caaatcaaca cttgctaaag caatttgtgg atttttagat ataacaggta atatccaatt
    19801 ttgtaatagg ggatttaatc aattgtcaat tagcgaacga tctgaattcg taggttatgt
    19861 gatgcaaaat ccaaatcata tgatttctga aaaaatgatt tacgatgaag tagcattagg
    19921 gttaagagca cgaggtatga aagaatcaga tataaagata cgagtagaga atgtgctcaa
    19981 aatatgcgga ctttatgcat ttcggaattg gccaattgca gctttaagct atggtcaaaa
    20041 aaagagggtc actatagcat ctgttttagt cttaaatccg gaaataatca tattggatga
    20101 accgactgct ggtcaagatt tctatcatta taatgagata atgtcatttt taattgaact
    20161 aaacagacag gggaagacga ttattatgat tacgcatgat atgcatttat tgtctgagta
    20221 tagttcaaga acagttgtat tatcaaaagg tcaagtcgtt gctgatacca cgccagtatt
    20281 ggttttaaat gataaaaaaa tctgtgagat tgcatcattg agacaaacat cgctatttga
    20341 aatggccgaa tatataggga ttagcgagcc acagaaatta gtacaattat ttattaacca
    20401 tgataggaag gtgagacgcc aatgaatcaa tataatacta taggttttca cccgggaaat
    20461 agtcgtattc atcaattaaa tgcgactgtt aaacttttat tcttattagt tgtttctatt
    20521 tctgcaatgg tgacttatga cacaagatat ttaattttaa ttagtgcttc atctatttta
    20581 ttggtcaaat atgctcatat tgaatggaaa caagttcgct ttgttgttaa attcattctg
    20641 tttttcacaa tattaaatat tattgccgtg tacatatttg accctgaata tggtgtgaag
    20701 atttataatc agcgtacaga gttagtcaat ggtattggtc gatttacgct aacatcacag
    20761 gaattattct atctttttaa tctaatatta aaatatatta gtacagttcc tttagcgtta
    20821 atatttttat tcacaacgaa tccgagtcat tttgctgcaa gtttaaatca gctaggtgtg
    20881 aattataaaa tcagttacgc agtctcacta gcattaaggt atattccaga tattcaagaa
    20941 acatatttta atatttcaca agcgcaacaa gcaagaggat atgataattc gaaaaaagca
    21001 aaatttacta gccgtgttaa aggtatcaaa cgcattgtgt tacctttaat attttctagt
    21061 atcgaaagaa ttgacactat tagtactgct atggagttaa gacaattcgg acagtataaa
    21121 aggagaacct ggtacgtcaa aaaacaatta aaaaaagatg attatgttgt tttgtgtttg
    21181 acgttaatac ttctgatgtt agtagttaca ttattctttt taaataatag tcgatatttc
    21241 aacccgtggc attagtattc atataaatag tctttaaata gaaataggag ggagacattt
    21301 aatgataaat actgaaagat taaatttaat gattccaagt tcctcgcatt taattgaact
    21361 ttataatatt tgtagtcatc cacaagcaaa tatatacact cccaaaggtt tacataattc
    21421 caaattagac acacaacggt ggattgaaaa atggcgaaac cattggcaac aatatcaatt
    21481 tggttacttt gtattggtaa aaaaaataga ttgtagtgtt attggtattt gtggatatga
    21541 atatcgacaa ttaaagcaag aaacagtatt aaatttattt tataaattac atccaagttt
    21601 tgaaggacaa gggtacgcat gtgaggctat tacagcaatc acaaattttg tgaattatat
    21661 cgatcaagaa acagtaaaag ttatcaggac aaataagtgt aaccaacgtt caataaattt
    21721 agcagaaagg cttaaattca agcgagacga tactatggac gacattatca atcaa
//
LOCUS       DANJFZ010000038        20828 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.038, whole genome shotgun sequence.
ACCESSION   DANJFZ010000038 DANJFZ010000000
VERSION     DANJFZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.038"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(186..575)
                     /locus_tag="PWN45_001230"
     CDS             complement(186..575)
                     /locus_tag="PWN45_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623005.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            932..1240
                     /gene="rpsJ"
                     /locus_tag="PWN45_001231"
     CDS             932..1240
                     /gene="rpsJ"
                     /locus_tag="PWN45_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL0623006.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            1268..1930
                     /gene="rplC"
                     /locus_tag="PWN45_001232"
     CDS             1268..1930
                     /gene="rplC"
                     /locus_tag="PWN45_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL0623007.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1957..2580
                     /gene="rplD"
                     /locus_tag="PWN45_001233"
     CDS             1957..2580
                     /gene="rplD"
                     /locus_tag="PWN45_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL0623008.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            2580..2855
                     /gene="rplW"
                     /locus_tag="PWN45_001234"
     CDS             2580..2855
                     /gene="rplW"
                     /locus_tag="PWN45_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL0623009.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2888..3721
                     /gene="rplB"
                     /locus_tag="PWN45_001235"
     CDS             2888..3721
                     /gene="rplB"
                     /locus_tag="PWN45_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL0623010.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3788..4066
                     /gene="rpsS"
                     /locus_tag="PWN45_001236"
     CDS             3788..4066
                     /gene="rpsS"
                     /locus_tag="PWN45_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL0623011.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            4095..4448
                     /gene="rplV"
                     /locus_tag="PWN45_001237"
     CDS             4095..4448
                     /gene="rplV"
                     /locus_tag="PWN45_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL0623012.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            4472..5125
                     /gene="rpsC"
                     /locus_tag="PWN45_001238"
     CDS             4472..5125
                     /gene="rpsC"
                     /locus_tag="PWN45_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL0623013.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            5128..5562
                     /gene="rplP"
                     /locus_tag="PWN45_001239"
     CDS             5128..5562
                     /gene="rplP"
                     /locus_tag="PWN45_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL0623014.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            5552..5761
                     /gene="rpmC"
                     /locus_tag="PWN45_001240"
     CDS             5552..5761
                     /gene="rpmC"
                     /locus_tag="PWN45_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL0623015.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5785..6048
                     /gene="rpsQ"
                     /locus_tag="PWN45_001241"
     CDS             5785..6048
                     /gene="rpsQ"
                     /locus_tag="PWN45_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL0623016.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            6080..6448
                     /gene="rplN"
                     /locus_tag="PWN45_001242"
     CDS             6080..6448
                     /gene="rplN"
                     /locus_tag="PWN45_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL0623017.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            6484..6801
                     /gene="rplX"
                     /locus_tag="PWN45_001243"
     CDS             6484..6801
                     /gene="rplX"
                     /locus_tag="PWN45_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL0623018.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6828..7367
                     /gene="rplE"
                     /locus_tag="PWN45_001244"
     CDS             6828..7367
                     /gene="rplE"
                     /locus_tag="PWN45_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL0623019.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            7390..7575
                     /locus_tag="PWN45_001245"
     CDS             7390..7575
                     /locus_tag="PWN45_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL0623020.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7607..8005
                     /gene="rpsH"
                     /locus_tag="PWN45_001246"
     CDS             7607..8005
                     /gene="rpsH"
                     /locus_tag="PWN45_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL0623021.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            8030..8566
                     /gene="rplF"
                     /locus_tag="PWN45_001247"
     CDS             8030..8566
                     /gene="rplF"
                     /locus_tag="PWN45_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL0623022.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            8597..8956
                     /gene="rplR"
                     /locus_tag="PWN45_001248"
     CDS             8597..8956
                     /gene="rplR"
                     /locus_tag="PWN45_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL0623023.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8977..9477
                     /gene="rpsE"
                     /locus_tag="PWN45_001249"
     CDS             8977..9477
                     /gene="rpsE"
                     /locus_tag="PWN45_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL0623024.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            9494..9673
                     /gene="rpmD"
                     /locus_tag="PWN45_001250"
     CDS             9494..9673
                     /gene="rpmD"
                     /locus_tag="PWN45_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL0623025.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9690..10130
                     /gene="rplO"
                     /locus_tag="PWN45_001251"
     CDS             9690..10130
                     /gene="rplO"
                     /locus_tag="PWN45_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL0623026.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            10130..11422
                     /gene="secY"
                     /locus_tag="PWN45_001252"
     CDS             10130..11422
                     /gene="secY"
                     /locus_tag="PWN45_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL0623027.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            11439..12086
                     /locus_tag="PWN45_001253"
     CDS             11439..12086
                     /locus_tag="PWN45_001253"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL0623028.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            12279..12497
                     /gene="infA"
                     /locus_tag="PWN45_001254"
     CDS             12279..12497
                     /gene="infA"
                     /locus_tag="PWN45_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL0623029.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            12529..12642
                     /gene="rpmJ"
                     /locus_tag="PWN45_001255"
     CDS             12529..12642
                     /gene="rpmJ"
                     /locus_tag="PWN45_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL0623030.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            12665..13030
                     /gene="rpsM"
                     /locus_tag="PWN45_001256"
     CDS             12665..13030
                     /gene="rpsM"
                     /locus_tag="PWN45_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL0623031.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            13054..13443
                     /gene="rpsK"
                     /locus_tag="PWN45_001257"
     CDS             13054..13443
                     /gene="rpsK"
                     /locus_tag="PWN45_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL0623032.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            13518..14462
                     /locus_tag="PWN45_001258"
     CDS             13518..14462
                     /locus_tag="PWN45_001258"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL0623033.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            14479..14847
                     /gene="rplQ"
                     /locus_tag="PWN45_001259"
     CDS             14479..14847
                     /gene="rplQ"
                     /locus_tag="PWN45_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL0623034.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            15385..16194
                     /locus_tag="PWN45_001260"
     CDS             15385..16194
                     /locus_tag="PWN45_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDL0623035.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            16191..17051
                     /locus_tag="PWN45_001261"
     CDS             16191..17051
                     /locus_tag="PWN45_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDL0623036.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            17041..17847
                     /locus_tag="PWN45_001262"
     CDS             17041..17847
                     /locus_tag="PWN45_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0623037.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            17852..18655
                     /gene="truA"
                     /locus_tag="PWN45_001263"
     CDS             17852..18655
                     /gene="truA"
                     /locus_tag="PWN45_001263"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0623038.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            18895..19332
                     /gene="rplM"
                     /locus_tag="PWN45_001264"
     CDS             18895..19332
                     /gene="rplM"
                     /locus_tag="PWN45_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL0623039.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            19346..19744
                     /gene="rpsI"
                     /locus_tag="PWN45_001265"
     CDS             19346..19744
                     /gene="rpsI"
                     /locus_tag="PWN45_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0623040.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            20289..>20828
                     /locus_tag="PWN45_001266"
     CDS             20289..>20828
                     /locus_tag="PWN45_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0623041.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFS"
BASE COUNT         7596 a         3268 c         4059 g         5905 t
ORIGIN      
        1 acacaaaaaa cgactgccaa tgattggtag tcgttttttt ctttgaagta aggataaaat
       61 aagaagcgaa ataacataaa gcgatattat gaatgtcttt aaatatccga tataaatata
      121 cttcttttaa aattgttgga cgtgtttctt ctatattata tacctaatta aaaaggtttt
      181 aagaattatt ctctatgtcc atctttgtat tgtgtgtaaa tgacacgagc atttttatta
      241 ggtatatagg ttggcgctga taatacaact aggacaaata gaatcattag gaaaaataaa
      301 atccaatgaa tcaaaattcc taagaagggg ataatagcta aaaaactacc tactatacca
      361 aatattggaa taatcgctat aggtctaatc gcattttgtc tattcactaa taatataata
      421 gtaagaatta gataaagtaa tgcattaaag agtaaaggct gccatccaaa acttaaaata
      481 aacgttgcac ctaaaaaagg aatgccatag aaaaactcac aaagtattaa aaagatacct
      541 ataagtgcta aagataattt tggtccattt ttcatattca ttcaccaccg ttcttatgac
      601 taattatata gaataatttt caatgataat aataaaatga ttcaaggtac gaatttacaa
      661 taaagaagga aataaaaaat atctcgaaaa tagttgaact gactaagtat ataaagtaaa
      721 atataaaagt atgtgttaga cagatagaaa aattatgtga aaagccttga tttatcgatg
      781 tttttcgtgt aatataacta acgttggact ttaagaaaag cgatgaagcg aaaggttact
      841 gacacacccg gccgctttgc catggcgctg tgtaagatag ttttcgtgga gaagtctatc
      901 actaaatgta gacgaataag gagggaaaat tatggcaaaa caaaaaatca gaatcagatt
      961 aaaagcttat gatcaccgcg taattgatca atcagcagag aagattgtag aaacagcgaa
     1021 acgttctggt gcagatgttt ctggaccaat tccgttacca actgagaaat cagtttacac
     1081 aatcatccgt gccgtgcata agtataaaga ttcacgtgaa caattcgaac aacgtacaca
     1141 caaacgttta atcgatattg taaacccaac accaaaaaca gttgacgctt taatgggctt
     1201 aaacttacca tctggtgtag acatcgaaat caaattataa tagacaattt taggaggtgg
     1261 actttcgatg accaaaggaa tcttaggaag aaaaattggg atgacacaag tattcggaga
     1321 aaacggtgaa ttaatccctg taacagtagt agaagctaaa gaaaatgttg tattacaaaa
     1381 gaaaactgta gaagttgatg gatacaacgc aatccaagtt ggatttgaag acaaaaaagc
     1441 atacaaaaaa gatgcaaaat ctaataaata tgctaataaa ccagctgaag gtcacgctaa
     1501 aaaagctgac gcagcaccta agcgcttcat tcgtgaattc cgcaatgtag acgtggatgc
     1561 ttacgaagta ggtcaagaag tctcagtaga tacttttgta gctggcgacg ttattgacgt
     1621 aacaggcgta tcaaaaggta aaggtttcca aggtgcaatt aaacgccacg gacaatctcg
     1681 tggacctatg tcacacggtt ctcatttcca cagagcacca ggttctgtag gtatggcttc
     1741 agatgcttct agagtattta aaggccaaaa aatgccagga cgtatgggtg gaaacactgt
     1801 aactgttcaa aacttagaag tagttcaagt tgacacagaa aacaaagtta tcttagtaaa
     1861 aggtaacgta cctggaccta aaaaaggttt agtagaaatc agaacttcaa ttaaaaaagg
     1921 taataaataa taagaagtga aaggaggaaa tgcataatgg ctaattatga tgttttaaaa
     1981 ttagacggaa ctaaatcagg ttcaatcgaa ttaagcgatg cagtattcgg tattgagcca
     2041 aataatagcg ttttattcga agctattaat ttacaacgtg cttcattacg tcaaggtacg
     2101 catgctgtta agaatcgttc agcagtaagc ggtggcggac gtaaaccatg gaagcaaaaa
     2161 ggaacaggtc gtgctcgtca aggtacaatc cgtgctccac aatggcgtgg cggtggtatc
     2221 gtattcggac caactccaag aagttatgca tacaaaatgc ctaagaaaat gcgtcgttta
     2281 gctttacgct cagcattatc tttcaaagct caagagaatg gcttaactgt agttgacgca
     2341 ttcaacttcg aagctccaaa aactaaagaa ttcaaaaatg tattatctac attagaacaa
     2401 cctaaaaaag tattagtagt tactgaaaac gaagatgtaa atgttgaatt atcagcacgc
     2461 aacatccctg gcgttcaagt gacaactgct caaggtttaa atgttttaga tatcactaat
     2521 gctgacagct tagtaattac tgaagctgct gctaaaaaag ttgaggaggt gctcggataa
     2581 tggaagcaag agatattctt aagcgccccg taatcactga gaaatcttct gaagcaatgg
     2641 ctgaagacaa atacactttc gacgttgata ctcgtgttaa caaaacacaa gtaaaaatgg
     2701 cagttgaaga aatcttcaac gtaaaagttg caagtgttaa tatcatgaat tacaaaccta
     2761 agaaaaaacg tatgggccgt taccaaggct atacaaacaa aagaagaaaa gcgattgtaa
     2821 ctcttaaaga aggatcaatc gacttattta actaataaaa caattaccat taaggaggta
     2881 agcgacaatg gctattaaaa agtataagcc aataacaaat ggtcgtcgta atatgacttc
     2941 gttagatttc gcagaaatca cgaaaactac acctgaaaag tcattattaa aaccgctacc
     3001 gaaaaaagcg ggacgtaaca accaaggtaa attgactgta agacaccatg gtggtggaca
     3061 caaacgtcaa taccgtgtta tcgatttcaa acgtaacaaa gatggtatca atgcaaaagt
     3121 tgattctatt caatatgatc caaaccgctc agcaaacatc gctttagttg tatatgcaga
     3181 cggtgaaaaa cgatatatca ttgctcctaa aggattagaa gtaggtcaaa tcgttgaaag
     3241 tggtgctgaa gctgacatca aagttggtaa cgcattacca ttacaaaaca ttccagttgg
     3301 tacagtagta cacaacatcg agcttaaacc tggtaaaggt ggacaaatcg ctcgttcagc
     3361 tggtgcaagt gctcaagtac ttggtaaaga aggtaaatac gtattaatca gattaagatc
     3421 tggtgaagtt cgtatgatct tatctacttg ccgtgctaca atcggtcaag ttggtaacct
     3481 acaacacgaa ttagttaacg ttggtaaagc cggacgttca agatggaaag gtatccgtcc
     3541 aacagttcgt ggttctgtaa tgaaccctaa cgatcaccca cacggtggtg gtgaaggtcg
     3601 tgctcctatc ggtagaccat ctccaatgtc accatggggt aaacctacgc ttggtaagaa
     3661 aactcgtcgt ggtaaaaaat catcagacaa acttatcgtt cgtggacgta agaaaaaata
     3721 atataatcaa cttatttggg tgtgcggctt aaagctgcac gcacataata agaagggagg
     3781 cgcccaaatg gctcgtagta ttaaaaaagg acctttcgtc gatgagcatt taatgaaaaa
     3841 agttgaagct caagaaggaa gcgaaaagaa acaagtaatc aaaacatggt cacgtcgttc
     3901 tacaattttc cctaatttca tcggacatac ttttgcagta tacgacggac gtaaacacgt
     3961 acctgtatat gtaactgaag atatggtagg tcataaatta ggtgagtttg ctcctactcg
     4021 tacattcaaa ggacacgttg cagacgacaa gaaaacaaga agataatatc tattaagtag
     4081 aggaggacat cctaatggaa gcaaaagcgg ttgctagaac aataagaatc gcacctcgta
     4141 aagtaagact agttcttgac ttaatcagag gtaaaaatgc tgctgaagct attgcaattt
     4201 taaaattaac aaacaaagct tcatcaccag taattgaaaa agtattaatg tccgctttag
     4261 ctaatgctga acataactat gacatgaaca cagatgaatt agtagttaaa gaagcatatg
     4321 ctaacgaagg accaacatta aaacgtttcc gtccacgtgc gcaaggtcgt gcaagtgcga
     4381 ttaacaaacg tacaagccac attacaatcg tcgtaagtga cggtaaagaa gaagctaaag
     4441 aagcttaatt aacttttaag gagggaatac tgtgggtcaa aaaattaatc caatcggact
     4501 tcgtgttggt attatccgtg attgggaagc taaatggtat gctgaaaaag acttcgcttc
     4561 acttttacac gaagatttaa aaatccgtaa atttattgat aatgaattaa aagaagcatc
     4621 agtttctcac gtagagattg aacgtgctgc aaaccgtatc aacattgcaa ttcatactgg
     4681 taaacctggt atggtaattg gtaaaggcgg ttcagaaatc gaaaaattac gcaacaaatt
     4741 aaatgcgtta actgataaaa aagtacacat caacgtaatt gaaatcaaaa aagttgatct
     4801 tgacgctcgt ttagtagctg aaaacatcgc acgtcaatta gaaaaccgtg cttcattccg
     4861 tcgtgtacaa aaacaagcaa tcactagagc tatgaaactt ggtgctaaag gtatcaaaac
     4921 tcaagtatct ggtcgtttag gcggagctga catcgctcgt gctgaacaat attcagaagg
     4981 aactgttcca cttcatacgt tacgtgctga catcgattat gcacacgctg aagctgacac
     5041 tacttacggt aaattaggcg ttaaagtatg gatttatcgt ggagaagttc ttcctactaa
     5101 gaacactagt ggaggaggaa aataataatg ttactaccaa aacgtgtaaa atatcgtcgt
     5161 caacatcgtc ctaaaacaac tggtcgttct aaaggcggta actacgtaac atttggtgag
     5221 tttggtttac aagctacaac aacgtcttgg atcacatctc gtcaaatcga atctgctcgt
     5281 atagcaatga cacgttacat gaaacgtggc gggaaagttt ggattaaaat cttcccacat
     5341 acaccatata ctaaaaaacc tttagaagta cgtatgggtg ctggtaaagg tgcggttgaa
     5401 ggctggatcg cagttgttaa accaggtaga attttattcg aagttgctgg cgtttctgaa
     5461 gaagttgcgc gtgaagcact acgtttagca agtcacaaac ttccagtaaa aactaagttt
     5521 gtaaaacgtg aggaattggg tggtgaaaca aatgaaagct aaggaaatta gagacttaac
     5581 cacttcagaa atcgaagaac aaatcaaatc ttcaaaagaa gagcttttta acctacgctt
     5641 tcagttagct acaggtcaat tagaagaaac tgcacgtatt cgtacagtaa gaaaaacgat
     5701 tgcacgtcta aaaactgttg ctcgtgaaag agaaattgaa caaagtaagg ctaatcaata
     5761 attaagtaag aggaggttac aaaagtgagc gaaagaaacg atcgtaaagt ttatgtaggt
     5821 aaagttgttt cagacaaaat ggacaagact attacagtac ttgttgaaac ttacaaaaca
     5881 cacaaattat acggtaaacg agtaaaatac tctaaaaaat acaaaactca tgatgaaaac
     5941 aattcagcta aattaggaga cattgttaaa attcaagaaa ctcgtccttt atcagcaaca
     6001 aaacgttttc gtttagtaga gattgttgaa gagtcagtaa ttatttaata caagtttaga
     6061 gataagggag gtttaactaa tgatccaaca agaaacacgc ttgaaagtag cagacaactc
     6121 tggtgctcgt gaagttctta caatcaaagt attaggtgga tctggtcgta aaacagcaaa
     6181 catcggcgat gttatcgtat gtactgttaa aaatgcaaca ccaggtggcg ttgttaaaaa
     6241 aggtgacgtt gtcaaagctg taatcgtacg tactaagtca ggtgttcgtc gtaatgacgg
     6301 ttcatacatc aaatttgatg aaaatgcatg tgttatcatc cgtgatgaca aaggcccacg
     6361 tggtactcgt atcttcggac ctgttgctcg tgaattacgt gaaggtaact tcatgaaaat
     6421 cgtatcatta gcaccagaag tactttaatt aataacaacc aatacaaata aggaggtgcc
     6481 cacatgcata tcaaaaaagg tgacaacgtt aaagttatcg caggtaaaga caaaggtaaa
     6541 gaaggtaaag taattgctac tctacctaaa aaagaccgtg tcgttgtgga aggtgttaac
     6601 attatgaaaa aacaccaaaa accaactcaa ttaaatcctg aaggtggaat cttagaaaca
     6661 gaggcagcaa tccatgtttc taatgtacaa ttattggacc ctaaaacaaa cgaaccaact
     6721 cgtgtaggtt acaaatttgt tgatggtaaa aaagttcgta tcgctaaaaa atctggcgaa
     6781 gaaattaaat ctaataatta ataacaagtt gaaaggagga tcccactttg aaccgtttaa
     6841 aagaaaagtt taacactgaa gttactgaaa acttaatgaa aaaattcaat tatagttcag
     6901 taatggaagt accaaaaata gataaaatcg ttgtgaacat gggtgtaggt gacgcagtac
     6961 aaaattctaa agtattagac aatgctgttg aagaattaga attgatcact ggtcaaaaac
     7021 cattagtaac taaagctaaa aaatcaatcg cgactttccg tttacgtgaa ggtatgccaa
     7081 tcggtgcgaa agtaacactt cgcggtgaaa gaatgtatga attcttagac aaattaattt
     7141 cagtatcatt accacgtgta cgtgacttcc aaggtgtttc taaaaaagca tttgacggac
     7201 gcggtaacta cactttaggt gttaaagaac aattaatttt cccagaaatc gactatgata
     7261 aagtaagtaa agttagagga atggatattg ttatcgtaac gactgctaac actgatgaag
     7321 aagctcgtga attgttagct aacttcggta tgccattccg taaataatta tttaaaggag
     7381 gctaattaag tggctaaaac ttcaatggtt gctaagcaac aaaaaaaaca aaaatatgca
     7441 gttcgtgaat acactcgttg tgaacgttgt ggccgtccac attctgtata tcgtaaattt
     7501 aaattatgcc gtatttgttt ccgtgaatta gcttacaaag gccaaatccc tggcgttcgt
     7561 aaagctagct ggtaataaaa aagagtctga aaggaggcaa caatcaatga caatgacaga
     7621 tccaatcgca gatatgctta ctcgtgtaag aaacgcaaac atggtgcgtc acgagaagtt
     7681 agaattacct gcatcaaata ttaaaaaaga aattgctgaa atcttaaaga gtgaaggttt
     7741 cattaaaaat gttgaatacg tagaagatga taaacaaggt gtacttcgtt tattcttaaa
     7801 atatggtcaa aacgatgagc gtgttatcac aggattaaaa cgtatttcaa aaccaggttt
     7861 acgtgtttat gcaaaagcta gcgaaatgcc taaagtatta aatggtttag gtattgcatt
     7921 agtatcaact tctgaaggtg taatcactga caaagaagca agaaaacgta atgttggtgg
     7981 agaaattatc gcatacgttt ggtaataaaa aataaggagg tgccataaca tgagtcgtgt
     8041 tggtaagaaa attattgaca tccctagtga cgtaacagta acttttgatg gaaatcatgt
     8101 aactgttaaa ggtcctaaag gtgaattatc aagaacttta aatgaaagaa tgacattcaa
     8161 acaagaagaa aacacaattg aagttgtaag accatctgat tctaaagaag atagaacaaa
     8221 ccatggtaca actcgtgctt tattaaacaa tatggtacaa ggtgtttctc aaggatacgt
     8281 aaaagtactt gaacttgttg gtgtaggtta ccgtgctcaa atgcaaggta aagacttaat
     8341 ccttaacgtt ggttattctc acccagtaga aattaaagct gaagaaaaca ttactttctc
     8401 agttgagaaa aacacagtcg ttaaagttga aggtatttca aaagaacaag ttggagcatt
     8461 agcatctaac atccgttcag taagacctcc agagccttac aaaggtaaag gtattcgtta
     8521 ccaaggtgaa tacgttcgcc gtaaagaagg taaaactggt aaataataga taaactctaa
     8581 agaaaggagt attgaaatga tcagtaaaat tgataaaaat aaagtgcgtt taaaaagaca
     8641 tgctcgtgtt cgtactaact tatcaggtac agctgaaaag ccacgtttaa acgtatatcg
     8701 ttcaaacaag catatctacg ctcaaattat tgatgataat aaaggcgtaa cattagctca
     8761 agcttcttca aaagacagcg acattgctac tacagcaact aaagttgaat tagcaactaa
     8821 agtcggtgaa gcaattgcta aaaaagctgc tgacaaaggc attaaagaaa tcgtatttga
     8881 ccgtggagga tatttatatc acggacgtgt taaagcatta gctgaagcag caagagaaag
     8941 cggattagaa ttttaattta aaggagggac aaatacatgg ctcgtagaga agaagagacg
     9001 aaagaatttg aagaacgcgt tgttacaatc aaccgtgtag caaaagttgt aaaaggtggt
     9061 cgtcgtttcc gtttcactgc attagttgta gttggagaca aaaatggtcg tgtaggtttc
     9121 ggtactggta aagctcaaga ggtaccagaa gcaatcaaaa aagctgttga agcagctaaa
     9181 aaagatttag tagttgttcc acgtgttgaa ggtacaactc cacacacaat tactggccgt
     9241 tacggttcag gaagcgtatt tatgaaaccg gctgcacctg gtacaggagt tatcgctggt
     9301 ggtcctgttc gtgccgtact tgaattagca ggtatcactg atatcttaag taaatcatta
     9361 ggatcaaaca caccaatcaa catggttcgt gctacaatcg atggtttaca aaaccttaaa
     9421 aatgctgaag atgttgcgaa attacgtggc aaaacagtag aagaattata caattaagga
     9481 gggaaaacta gttatggcta aattacaaat taccctcact cgtagtgtta ttggtcgtcc
     9541 tgaaacacaa cgtaaaactg ttgaagcttt aggtcttaaa aagactaaca gttcagtagt
     9601 tgttgaagat aaccctgcta ttcgtgggca aatcaacaaa gttaagcact tagtaacagt
     9661 agaagaaaaa taatcaagga ggtgccgaaa tgaaattaca tgagttaaaa ccggcagaag
     9721 gttcacgtaa agaacgcaat cgtgttggac gtggtgttgc gacaggtaac ggtaaaacaa
     9781 gtggtcgcgg acacaaaggt caaaaagctc gttcaggcgg tggtgtaaga ccaggatttg
     9841 aaggtggtca attaccatta ttccgtcgtt taccaaaacg tggttttact aacataaatc
     9901 gtaaagaata tgctattgtt aacttagacc aacttaataa atttgaagat ggtactgaag
     9961 taactccagc tttattagta gaatctggtg ttgttaagaa tgaaaaatct ggtatcaaaa
    10021 tactaggtaa tggttcactt gataagaaat tgacagtgaa agctcataaa ttctcagctt
    10081 cagcagcaga agctattgat gctaaaggtg gagcacacga ggtgatctaa tgattcaaac
    10141 ccttgtgaac ttctttagaa caaaagaggt tcgtaacaag attttcttca cactagcaat
    10201 gttagtaatt tttaaaatag ggacttatat accagctcca ggagtaaatc ctgcagcttt
    10261 tgataatccc caaggttctc aaggtgccac tgagttatta aatacttttg gtggcggagc
    10321 cttgaaacga ttttctattt ttgcaatggg tattgtaccc tacatcactg catcaatcgt
    10381 aatgcaatta ttacaaatgg atattgtccc taaattctca gaatgggcaa aacaaggtga
    10441 agtaggtaga agaaagttaa ataacgttac tcgttattta gcaatttctt tagcatttat
    10501 ccaatctata ggtatggcat tccaatttaa taattatctc aaaggtgcgc tgattatcaa
    10561 tcagtcaatt atgagttatt tattaatagc actagttttg acagcaggaa ctgctttctt
    10621 aatatggctt ggtgatcaaa tcactcagtt cggtgttggt aatggtattt ctattatcat
    10681 atttgcgggt attttatcaa cgttgccagc cagtctaatt caatttggac aaactgcgtt
    10741 cgttgggcaa gaagatacat cattagcatg gttgaaagta ttaggacttt tagtgtcact
    10801 gatactatta actgtaggtg ctatttatgt acttgaagcc gttcgtaaaa ttccgattca
    10861 atatgctaag aaacaaactg ctcaaagatt aggttcacag gcaacttatc tacctttaaa
    10921 agtgaactca gccggtgtta ttcctgtaat ctttgcaatg gcgttcttct tactgcctag
    10981 aacattaacg ttgttctatc cagataaaga atgggcacaa aacattgcga atgctgcaaa
    11041 tccttcaagt aatgttggta tggttgtata tattgtacta attatactat tcacatactt
    11101 ctatgcattt gtacaagtta atccggaaaa aatggctgat aaccttaaga aacaaggtag
    11161 ctatgttcca ggtattagac ctggtgaaca aaccaaaaaa tatattacta aagttttata
    11221 tcgtttaact tttgtaggtt ctatcttctt agccgttatt tcaattctac cgatattagc
    11281 aactaagttt atgggattac cacaatcaat tcagattggt ggtacaagtt tacttatcgt
    11341 tattggtgta gcgattgaaa caatgaaatc tttagaagct caagtatctc aaaaagaata
    11401 taaaggcttt ggtggtagat aattttagga gggcacttat gaatatcatt ttgatgggtt
    11461 tacctggcgc aggtaaagga actcaagcaa gtgaaattgt caagaaattc ccaatacccc
    11521 acatttcaac tggtgacatg ttcagaaaag ctataaaaga agaaactgaa ttaggtaaag
    11581 aagctaagtc ttatatggac cgtggcgaat tagttcctga tgaagtgact gtaggtatcg
    11641 ttaaggaaag aatttctgaa gacgatgcaa aaaaaggctt tttattagat ggcttcccaa
    11701 gaacaatcga gcaagctgag gcattaaata atattatgtc tgagcttgac agaaacattg
    11761 atgctgtcat caatatcgaa gttccggaag aagaattaat gaaccgtctt acaggtcgtc
    11821 gaatctgtga gtcatgtggt acaacgtatc atcttgtatt taatcctccg aaggtcgaag
    11881 gtatttgtga tatcgatggt ggtaaattgt atcaacgaga agatgataat cctgaaacgg
    11941 tagctaatcg tttgagtgtt aatattaaac aatctaagcc tattttagat ttctatgatc
    12001 aaaaaggtgt attgaaaaat attgatggtt caaaagatat tagcgacgtt accaaagatg
    12061 tcattgatat tttagatcat ttgtaataga tcaactttta gcggtctatt ttaagtgagg
    12121 catgatttaa tcatattaga tagatgagag tggcaattaa cgataattaa ttcccgtaat
    12181 ttttgtttaa cttataaaat taatcactga tagattcgta acaataaagt tgtctattga
    12241 cgattacctt actattgcaa aaagggggaa gttaatcaat ggctaaacaa gatgtaattg
    12301 aattagaagg tactgtatta gatactttac cgaacgcaat gtttaaagta gaattagaaa
    12361 atggtcatga gattttagct cacgtaagtg gtaaaatcag aatgaattac attcgtattc
    12421 tacctggcga caaagtaact gttgagatgt ctccgtacga tttaacacgc ggaagaatta
    12481 cttatcgtta taaataatcg tcactccata atatagggag gtataaaaat gaaagtaaga
    12541 ccatcagtaa aacctatttg cgaaaaatgt aaagtcatta aacgtaaagg taaagtaatg
    12601 gtaatttgtg aaaatccaaa acacaaacaa agacaaggtt aataaaagag aggtgtaaat
    12661 taatatggca cgtattgcag gagtagatat tccacgtgaa aaacgcgtag ttatctcatt
    12721 aacttatata tacggtatcg gtacgtcaac tgctcaaaaa attcttgaag aagctaacgt
    12781 atcagctgat actcgtgtga aagatttaac tgatgacgaa ttaggtcgca tccgtgaagt
    12841 tgtagacggt tataaagtcg aaggtgactt acgtcgtgaa actaacttaa atatcaaacg
    12901 tttaatggaa atttcatcat accgtggtat ccgtcaccgt cgtggtttac cagttcgtgg
    12961 tcaaaaaacg aaaaacaacg cgcgtactcg taaaggacca gttaaaacgg tagctaacaa
    13021 gaaaaaataa taggtaaagg aggcaaattt taaatggcac gtaaacaagt atctcgtaaa
    13081 cgtagagtga aaaagaatat tgaaaatggt gtagcacaca tccgttcaac attcaacaac
    13141 actattgtaa ctatcactga tgagttcggt aatgctttat catggtcatc agctggtgca
    13201 ttaggattca aaggatctaa aaaatcaaca ccatttgcag cacaaatggc ttctgaaact
    13261 gcatctaaat cagctatgga gcatggttta aaaacagttg aagtaacagt taaaggacct
    13321 ggtccaggtc gtgaatcagc tattcgtgca ttacaatctg caggtttaga agtaactgcg
    13381 atcagagacg ttactccagt acctcataac ggttgtcgtc caccaaaacg tcgtcgtgta
    13441 taatttatga tggtattgtt acaggtcact gagcaaacat tttaaattaa gtcgacgtat
    13501 ataaggagga tatttaaatg atagaaatcg aaaaacctag aattgagaca attgaaatta
    13561 gtgaagatgc taaattcggt aagttcgttg ttgaaccact agaacgtggc tacggtacta
    13621 cactaggaaa ctccttacgt cgtatcctac tatcttcatt accaggtgca gccgttaagt
    13681 atattgaaat tgagggagtt ttacatgaat tctcagcagt agacaatgta gttgaagatg
    13741 tttctacaat tattatgaac attaaacaat tagcattgaa aatttactct gaagaagata
    13801 aaactttaga aattgatgta cgtgatgaag gcgaagtaac agcaagcgac attacacatg
    13861 atagtgatgt tgaaatttta aacccagagc ttaaaattgc aacagtatct aaaggtggtc
    13921 acttaaaaat tcgtctagtt gctaacaagg gtagaggtta cgcattagca gaacaaaata
    13981 atactagtga tttaccaatt ggtgtaatcc ctgttgattc attgtattca cctgttgaac
    14041 gtgtgaacta tactgttgaa aatacacgtg taggtcaaag cagtgatttt gataaattaa
    14101 cattggatgt ttggactaat ggttcaatca caccacaaga atcagtttca ttagcagcaa
    14161 aaataatgac tgaacacttg aatatcttcg ttggtcttac tgatgaagcg caaaacgctg
    14221 aaatcatgat tgaaaaagaa gaagatcaaa aagaaaaagt attagaaatg tctattgaag
    14281 aattagactt atctgtacgt tcatataact gcttaaaacg cgcaggaatc aattctgttc
    14341 aagagttagc tgacaaatct gaagctgaca tgatgaaagt gcgtaattta ggtcgtaaat
    14401 ctttagaaga agttaaatac aaattagaag atttaggatt aggattaaga aaagaagatt
    14461 gataaaggag gttaactcat gggttacaga aaattaggtc gtacttctga tcaacgtaaa
    14521 gctatgttac gtgacttagc tacatcactt attattagtg aacgtattga aactacagaa
    14581 gctcgtgcaa aagaagttcg cagtgttgtt gagaaattaa tcactttagg taaaaaagga
    14641 gatttagctt ctcgtcgtaa tgcagctaaa actttacgta atgttgaaat cttaaacgaa
    14701 gatgaaacta cacaaactgc acttcaaaaa ttatttggtg aaatcgcaga gcgttacaca
    14761 gaacgtcaag gtggttacac tcgtatcctt aaacaaggcc ctcgtcgcgg tgacggtgct
    14821 gaatcagtaa ttatcgaatt agtataagaa tttatataaa tacactaact acttttatat
    14881 aaagcaaatg agtgcaacga taatgtttgc cacatacaaa tattgtctag ctcagagtac
    14941 cccatcaact aaataaagat ttaaagcgtg aactcaacaa acttatgatg gggtgcgcgc
    15001 ttttttattg aataaaatat cggacttatg aacagaaatg tttttaagtc cgatttcttt
    15061 atatttactt ttttgagtgc gaatctaaaa atatgatgaa gctagtttga tcaatttcac
    15121 tagaactgaa tgaagtgaaa gtattatcgt tgaatcaaac gcgaccaccc ctatattagt
    15181 aaatcattta ttttattaac gtttatacat aattgttaga gaggaataaa agtccttatc
    15241 attagttgtt aaacaacact ttaaagacta gaaatgagtc agaaaatact agtaatatat
    15301 agagaaccca gtcaatgtca tatacagcga tttataaata ttgtaaaata tgatagatac
    15361 acgaatcgta aaggagagag acaagtggag gataagaatt cagttattgt atttaaaaat
    15421 gtttcatttc aatatcaaag tgatgcatcc ttcacattga aagatgtttc ttttaatata
    15481 cctaaaggtc agtggacatc tattgttggt cataacggtt ctggaaaatc tacaattgcc
    15541 aagttaatga ttggcataga gaaagttaaa tctggagaaa ttttttataa taatcaagct
    15601 ataactgatg ataattttga aaagttaaga aaagacatag gaattgtatt tcagaatccg
    15661 gataatcaat ttgttggttc aattgtaaaa tacgatgtgg catttggact cgaaaatcat
    15721 gcggttccat atgacgaaat gcatagaaga gtcagcgaag cacttaaaca agttgatatg
    15781 ttagaacgtg cagattatga acctaatgca ttatcggggg gacagaagca gcgtgtggct
    15841 atagcaagtg tattagcact taacccctct gtcattatat tagatgaggc gacttctatg
    15901 ttagatcctg atgcacgtca aaatttattg gatttagtga gaaaagttaa atcagaacat
    15961 aatattacaa tcatttctat tacgcatgat ttatctgagg cgatggaagc agatcatgtt
    16021 atcgttatga ataaagggac tgtctataaa gaaggcacag cgactgaaat tttcgacaat
    16081 gcagaagagt taacaagaat aggtctagat ttgccattcc caatcaaaat aaatcaaatg
    16141 ctgggacacc aaacatcatt cttaacttat gaagggctgg tggatcaact atgactatac
    16201 ggtttgacaa tgtaagttat acctatcaaa aagggacacc atatcagcat caagctattc
    16261 atgatgttaa tacagaattt gaacaaggta aatattacgc catcgttgga caaacgggta
    16321 gtggtaaatc aacgttgata caaaatatta atgcgctgtt aaagccgact actgggacag
    16381 ttacagttga tgacattact attacacata agaccaaaga taaatatatt agacctgtaa
    16441 gaaaaagaat tggaatggta tttcaatttc ccgaatctca attatttgag gacacagtag
    16501 agcgtgaaat gatatttgga cctaaaaact ttaaaatgaa tttagatgaa gccaaaaact
    16561 atgcccatcg tctgttgatg gatttaggct tttcaagaga tgtaatgtca caatcaccat
    16621 ttcaaatgtc aggtggacaa atgcgtaaaa tagcgattgt atcgatattg gcaatgaatc
    16681 ctgatattat cgtggttgat gaacctacag caggacttga tccacaaagt aaacgacaag
    16741 taatgagatt actaaagtca ctacaaacag atgaaaataa ggcaattatc ctaatttcac
    16801 atgatatgaa tgaagtcgcg cgttatgcag atgaagtcat cgttatgaaa gaaggtagta
    16861 tagtgtcgca aacatcacca aaagagctct tcaaagataa aaaaaaattg gcggattggc
    16921 atattggttt gccagaaatt gttcagttac aatatgactt tgaacaaaaa tatcaaacaa
    16981 aattaaaaga tattgcctta actgaagaag catttgtaag cttgtataag gagtggcaac
    17041 atgaaaaata aattgattat agggcgatat ttaccaatta attcttttgt tcatcatctt
    17101 gatccaagag caaagttaat gtttgttttt ctttttatta tattaatatt tttctgtcat
    17161 tcaccattaa catatctttg ggtgtttgca cttatcttat tcattatgag attagcaaaa
    17221 attcaattat ggttcctaat caaaggttta acaccaatat ttttcttttt aatcttcaca
    17281 ttaatgatgc atatattttt aactaaaggt ggatatgtat tagttgaatg gcatggcatc
    17341 acgattgaaa ctaatggtat tttagaaggg ctatatatat cactgcgttt aattgggatt
    17401 gtaatgattg caacaattat gacactatct acaagtccaa ttgatttaac agatgcgttt
    17461 gaaagattac ttgcaccact aaagatgttt aaattaccag ttcatcaatt aagtatgata
    17521 atgtccattg cgttacgatt catcccgacg ttaatggatg agttagataa aatcattttg
    17581 gcgcaaaagt cgcgtggttc tgaaataagt tcagggaaca ttgcaacacg tattaaatca
    17641 tttattccat tactagtgcc actattcatc tcagctttcc aacgcgccga agaattagcg
    17701 gtcgcaatgg aagttagggg ttatgatgcc aatgttaaga gaacgagcta cagacagctt
    17761 aaatggcaat tgagagatac gatatcttta accatgatta tcccaattgc aattatttta
    17821 ttcgtattaa aatattcagg agtgtaaatt catgcgtata ttagtagaaa ttgcgtatca
    17881 aggaaataat tttctaggtt ttcaaattca acaaaatggt cgaacggtac agcaacaatt
    17941 tgaaaagcta ttacaacgca tgcataaaag acatgtgaga atccatcctt caagtaggac
    18001 tgatagaggt gtacatgcga tacagcaata ctttcatttc gacacagagc tgaatatacc
    18061 tatgtcacaa tggcaatatg caatgaaccg aacgttacct gatgatattt atgtcaacaa
    18121 tgtagttaca gtcgatgacg attttcattg tcgttatgat tgtgtaggta aacgttatcg
    18181 ctacaaagta tatcaagcac aacaccgcga tccatttcaa agtggtttga aaacatttat
    18241 tcctgaaacg ttggatttag gtaaaatgaa cagagcggcg caacaattca taggtacaca
    18301 tgattttacg ggattttgtt cgcaaaaaac tgaagtagaa agcaaagtta gaacattata
    18361 ccaaagtgaa atcgtaaaga cagatgatgg ctttgattat attgtcactg gatctggatt
    18421 tttatataac atggtgcgtg tcttagttgc atttttaata gaagttggaa aagggcgaca
    18481 tgaagtatct gatgtaccaa aacttcttga atcaaagaat cgtaagaatg tacctttcac
    18541 tgcaccggct gaaggattat atttagagaa aatttattta gatgaaaatg agttattaaa
    18601 agactttggt aacgatatca aaatacatcg taaaaaatca ttacaaaatg actgattgcc
    18661 attgacaaaa agcttgtgaa attataagat tattaacggt attgttttat atccacccca
    18721 cgataagccc cggaaactta ttgtgttaca agatatataa gcagaaacga acaacagtta
    18781 acaaaataaa tgaaattaaa cgttttaaaa atgaaacaaa tgaaatcatc tattaggtta
    18841 tgaaactgtt tatagcttga atagaagcat ttatttttta ggaggacaat tattatgcgt
    18901 caaacattta tggcaaatga atcaaacatt gagcgcaaat ggtatgttat cgatgctgaa
    18961 ggccaaacat taggtcgttt atcatcagaa gtagcatcta tcttacgcgg taaaaataaa
    19021 gtaacttaca caccacacgt tgatactggt gattatgtaa tcgttattaa tgcatcaaaa
    19081 atcgaattta ctggtaacaa agaaactgac aaagtttact accgtcactc aaatcaccca
    19141 ggtggtatca aatcaatcac tgctggtgaa ttaagaagaa ctaacccaga acgtttaatt
    19201 gaaaactcaa ttaaaggtat gttaccaagc actcgtttag gcgaaaaaca aggtaaaaaa
    19261 ttatttgtat atggtggcgc tgaacatcca cacgctgcac aacaaccaga aaactacgaa
    19321 ttacgtggtt aattagaagg aggaaatgac tttggcacaa gttgaatata gaggcacagg
    19381 ccgtcgtaaa aactcagtag cacgtgtacg tttagtacca ggtgaaggta acatcacagt
    19441 taataaccgt gacgtacgcg aatacttacc attcgaatca ttaattttag acttaaacca
    19501 accatttgat gtaactgaaa ctaaaggtaa ctatgatgtt ttagttaacg ttcatggtgg
    19561 tggtttcact ggacaagctc aagctatccg tcacggaatc gctcgtgcat tattagaagc
    19621 agatcctgaa tacagaggtt ctttaaaacg cgctggatta cttactcgtg acccacgtat
    19681 gaaagaacgt aaaaaaccag gtcttaaagc agctcgtcgt tcacctcaat tctcaaaacg
    19741 ttaattgtcg gacgatatat acaaaacacc tcgatattat gtcgaggtgt ttttttgcgt
    19801 ttttgcggcg aatatggaat gtgtagaata taaatgaatt tttaccttcc caccataaaa
    19861 gatgaagaac catgaaatgt ggagaacaat aaatagttga tattctgtta tttttttgga
    19921 agtgaagtga tttgaatact ttactctaaa cgataaaagg ttaaaaaaac aacaaaaaga
    19981 aaatattact gtcataattg gaaatacacc gtattctgct ggtcaatcta gtgtaaataa
    20041 tgataataaa aaaatgaata tgaaaaatta aatgcaaaaa tagcaaatac ctatgcaaat
    20101 ttttcaacag caaatttaaa aaaatcatta tatgattcgt atatacaggc ttttcgatgg
    20161 gcaactgata gagttggaaa aaaggtatta taggatttgt aacaaatgga tcatttattg
    20221 atagtcaaag cactgatgga tttagaaaag ttttatacga tgaatttaac tatctgtaca
    20281 taattaattt gagaggcgac caacgaacac agggtgaaaa atcaagaaaa gaaggtggaa
    20341 aaatatttgg atcaggaagt agagcaccga tagctatatc tattttagtt aaagatggaa
    20401 gttacaatca cgatatctat tataatgaca ttggtgaata tttaactaga gagcaaaaat
    20461 tagatacttt aatgaaacat caatcaattg tgaatttgaa aagtttgaat gttttacccg
    20521 ataaaaacaa tgattggatt aatcaacgag acataaacta tgagaattat ttaccaatgt
    20581 atgattccaa ggatattgaa aattctatat atttagatca atttaatgga gtaaattcag
    20641 caagagataa ttgggtaaca aatttttcga atgaaaaagc attagtaaat gcaaaattac
    20701 ttgtagataa ctataattct gaaatagata gattaattga tatcttagat tcaagagaac
    20761 gtataaattt agtgaacaaa gatgaaacat ttattagttg gacacgagga cttacccaaa
    20821 aattttct
//
LOCUS       DANJFZ010000039        20543 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.039, whole genome shotgun sequence.
ACCESSION   DANJFZ010000039 DANJFZ010000000
VERSION     DANJFZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.039"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            35..1375
                     /locus_tag="PWN45_001267"
     CDS             35..1375
                     /locus_tag="PWN45_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0623042.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(1581..1913)
                     /locus_tag="PWN45_001268"
     CDS             complement(1581..1913)
                     /locus_tag="PWN45_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDL0623043.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(1997..3142)
                     /locus_tag="PWN45_001269"
     CDS             complement(1997..3142)
                     /locus_tag="PWN45_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL0623044.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(3351..3730)
                     /gene="rnpB"
                     /locus_tag="PWN45_001270"
     ncRNA           complement(3351..3730)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWN45_001270"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(3793..4137)
                     /gene="gpsB"
                     /locus_tag="PWN45_001271"
     CDS             complement(3793..4137)
                     /gene="gpsB"
                     /locus_tag="PWN45_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL0623045.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(4151..4714)
                     /locus_tag="PWN45_001272"
     CDS             complement(4151..4714)
                     /locus_tag="PWN45_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL0623046.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(4707..5057)
                     /locus_tag="PWN45_001273"
     CDS             complement(4707..5057)
                     /locus_tag="PWN45_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDL0623047.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            complement(5112..5291)
                     /locus_tag="PWN45_001274"
     CDS             complement(5112..5291)
                     /locus_tag="PWN45_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623048.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            5546..6223
                     /gene="recU"
                     /locus_tag="PWN45_001275"
     CDS             5546..6223
                     /gene="recU"
                     /locus_tag="PWN45_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL0623049.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            6220..8403
                     /locus_tag="PWN45_001276"
     CDS             6220..8403
                     /locus_tag="PWN45_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0623050.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(8969..9310)
                     /locus_tag="PWN45_001277"
     CDS             complement(8969..9310)
                     /locus_tag="PWN45_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623051.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(9315..9974)
                     /gene="nth"
                     /locus_tag="PWN45_001278"
     CDS             complement(9315..9974)
                     /gene="nth"
                     /locus_tag="PWN45_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL0623052.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(9964..10650)
                     /locus_tag="PWN45_001279"
     CDS             complement(9964..10650)
                     /locus_tag="PWN45_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDL0623053.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            complement(10978..12270)
                     /gene="asnS"
                     /locus_tag="PWN45_001280"
     CDS             complement(10978..12270)
                     /gene="asnS"
                     /locus_tag="PWN45_001280"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL0623054.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(12592..15285)
                     /locus_tag="PWN45_001281"
     CDS             complement(12592..15285)
                     /locus_tag="PWN45_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HDL0623055.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(15309..16280)
                     /locus_tag="PWN45_001282"
     CDS             complement(15309..16280)
                     /locus_tag="PWN45_001282"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL0623056.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(16267..17469)
                     /locus_tag="PWN45_001283"
     CDS             complement(16267..17469)
                     /locus_tag="PWN45_001283"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL0623057.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(17474..18616)
                     /gene="bshA"
                     /locus_tag="PWN45_001284"
     CDS             complement(17474..18616)
                     /gene="bshA"
                     /locus_tag="PWN45_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDL0623058.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(18857..19174)
                     /locus_tag="PWN45_001285"
     CDS             complement(18857..19174)
                     /locus_tag="PWN45_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0623059.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(19511..20191)
                     /locus_tag="PWN45_001286"
     CDS             complement(19511..20191)
                     /locus_tag="PWN45_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL0623060.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(20263..>20543)
                     /locus_tag="PWN45_001287"
     CDS             complement(20263..>20543)
                     /locus_tag="PWN45_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDL0623061.1"
                     /translation="DITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLN
                     DYIDYFHLQFPYYDFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
BASE COUNT         6659 a         3640 c         2885 g         7359 t
ORIGIN      
        1 ttgacaccgt ataatagcca attgggaggg aaatatgaaa aatacattta atccattaca
       61 aagattacat ttctatgcag caatatttat tgctccactg ttaatcactt taaccatttc
      121 gggcattggt tacttgttct ttccagaagt tgaaaataat atttataaga atgagttttt
      181 tggtgacagt gatgtaaaaa cgcatcaaac attaaatgat gcagtacatc aagttgaaca
      241 acaatatgaa ggattctttg taagtaaagt tagcatactt gatgaaccat ataacaaacg
      301 aattacactg agtgatatgg caggaaatca acgttacgtc tttctagatc ataacaatca
      361 atttgttgca gatcaaaatg cgaaacatac gtattctaat gtgatgcgaa gtatacatag
      421 ttctttgttt actgaaaata ctattattaa ttatttagta gagttaaccg catgttggat
      481 gatattcatg attttatctg gtacttattt actcattaag aagcatttaa tttctaacaa
      541 aagtaaggca cttcgttggc aaaagtggca cgcaatgatt ggagttatca ttgcaattcc
      601 agtatttgta ttagtcttaa ctggattgcc atggtctggt tttatgggca gtaaaattgc
      661 cggtatgatg gacacaaacg gtgcccttgg tcaaggtgaa ttagcgatta atccacctaa
      721 atcagatttg aacgaattac cttgggctac acgtaaaaat aaacagccag cttcatccga
      781 aaaaggttca agtggtcatc atggtaatgc agcaatgcct caaaccaaat tagattatca
      841 aatatctatt gataaggtcg ttgaacaggc gcaaaaagct ggtattaaaa agccgttttc
      901 aatcgtatat ccaagtgata aaaatggtac ctttattgta tctaatacta gtaattcagg
      961 tgttactggg ctagatgtat caccatacaa ggaacaaaca ctttatttcg atcaatatag
     1021 cggtaaaaag ctaggtacga ttaaatatga tgactacggt attattgcta aatggtttac
     1081 atggggcatt ccgcttcacg aaggtcattt attcggcatt ttaaataaaa tcattaattt
     1141 atttgtatgt atcgctttat tagtagccat tggcatgggg tttgtctctt ggataaagcg
     1201 tacaaaaaat actgcaataa aagtaccaca tcgcgtaaaa aaaccagcat ctatatcact
     1261 cataatatgt ttaattgtat taggattatt aatgccatta tttggattat cacttatcct
     1321 tgtatttata attgaattaa tattatatat taaagatcgt cgtgctaaac aataatgcac
     1381 ttaaagtttt gaactgacga aatttacaaa atgattctcg tctctctaat tacttaaaac
     1441 gggttctata ataaatcgta ctgatggaaa gtttttactt tttatctgtc cgattttttg
     1501 aatttgaaga taaaaaaaca tctaaaacgc ataagaagat aactctactt cttcaaaatt
     1561 acgttataga tgctttttta ttaattcata tgcatgtcgt gaattttcat cattaaaccg
     1621 tgtgggacgt catcatgttt aacaatctct tgtttataaa atgtcttccc atcatcttta
     1681 gaaactaatt ttacaaagtc tccttttttc ggcttgaaat cattagatgg ttctactttg
     1741 atattatgtt tgactaatcc atctttttca ctgacaactt tttctgcatt tgtattacta
     1801 ttcatataac caaaataagt cgttttttga ctagaaaaaa tcattaaaca tacaacggca
     1861 ataccaataa caaccaatgc aatagataaa cctagaccca tttttttacc catttataga
     1921 ctccttcatt tttcaaaaat acctattatc atgagaactt aattattact aattttagtc
     1981 acgcattaga aacactttag ttttcaatag ttttacgttc tgtcttttta ccccagtatt
     2041 gataataagt acattctata tatccattaa ataacttacg tcgctttgtt gctttacgat
     2101 ctactaaata ttcaaattct ttattacttg ttaaaatgta tgtagataaa aatggatgtt
     2161 gtttcattag tttaccaata taacggtaca tttcttcaac ttcttcacga tcaccaatac
     2221 gttcaccata tggaggattt ccaattaacg ccaccggttc ttctgtatca attgttaatg
     2281 tattgacatc ttttacacta aatttaataa tatcagacaa tccaacttct tcagcgttac
     2341 gcttagcaat ctctaccatt tctggatcga tatcagaagc atatacttcg atttctttat
     2401 cataatcagc catcttatcc gcttcatcac ggtaatcatc ataaatattt gctggcatga
     2461 tgttccattg ctctgatacg aactcgcgat taaaaccagg tgcgatattt tgagcaatta
     2521 aacaagcttc tatagctatt gttcccgaac cgcaaaatgg atcaattaaa ggtgtatcac
     2581 ctttccagtt tgcaagacgg attaaacttg ctgccaacgt ttctttaatt ggtgcttcac
     2641 cttgtgctaa tctataacca cgtctgttca aaccagaacc tgatgtgtcg atagtcaata
     2701 atacattatc ttttaaaatg gcaacttcaa cagggtattt ggcacctgat tcatttaacc
     2761 aacctttttc gttatatgcg cgacgtaatc gttcaacaat agctttctta gttatcgcct
     2821 gacaatctgg cacactatgt agtgttgatt taacgcttct accttgaact gggaagttac
     2881 cctctttatc aattatagat tcccaaggga gcgctttggt ttgttcgaat aattcgtcaa
     2941 acgttgttgc gttaaaacgt ccaacaacaa ttttgattcg gtctgctgtg cgcaaccata
     3001 aatttgcctt tacaattgca cttgcgtctc cttcaaaaaa tatacgacca ttttcaacat
     3061 ttgtttcata gcctaattct tgaatttccc tagcaacaac agcttctaat cccatcggac
     3121 aaactgcaag taattgaaac atatatgatt ctccttttat acaggtattt tattcttagc
     3181 ttgtgttttt tatacatttc caacaaattt aatcgctgat acattaacgc atccgcttac
     3241 tattttaaaa caaggcagtg tcattatatc aagacaaggc gttaatttta agtgttcttt
     3301 tcatgaaaaa agctctccat catctaggag agctaaacta gtagtgatat ttctataagc
     3361 catgttctgt tccatcgtac tcatcacgtg cactagtcac actggtactc aggtgataac
     3421 catctgtcta caccacttca tttcgcgaag tgtgtctcgt ttatacgttg aattccgtta
     3481 aacaagtgct cctaccaaat ttggattgct cactcgaggg gtttaccgcg ttccaccttt
     3541 tatatttcta taaaagctac gtcactgtgg cactttcaaa ttactctatc catatcgaaa
     3601 gacttaggat atttcattgc cgtcaaatta atgccttgat ttattgtttc atcaagcacg
     3661 aacactacaa tcatctcaga ctgtgtgagc atggactttc ctctatataa tatagcgatt
     3721 acccaaaata tcacttttaa aattataaca tagtcattat tagtaagaca gttaaacttt
     3781 tgtatttagt aattatttac caaatacagc tttttctaag tttgaaatac gttttaaaat
     3841 atctacatta tttgaagatg tatttgttgt tgtattattc gaagaaaaac ttttattgtc
     3901 ctgaggtctt gatgttgcta cacgtagtct taattcttct aattcttttt taagtttatg
     3961 attctcttct gataatttta caacttcatt attcatatcg gccatttttt gataatcagc
     4021 aataatgtca tctaaaaatg catctacttc ttctcttcta tagccacgag ccatcgtttt
     4081 ttcaaaatct ttttcataaa tatcttttgc tgataatttc aatgaaacat ctgacatttt
     4141 ttccacctca ttagaaactt tgatcttcag accactgtaa gtcattgatg aatgctgtta
     4201 attcatcgaa cgtcacaata tcacaagtat agtttgtttt atccataaaa tcaactaaca
     4261 tctgcttgaa gaacttagga ctcgcctctt gttcctcatc ataaatgagc aatgtttgat
     4321 ccgaatgttc aagcataaat tgatctgctt gtttaaattg aaaaggacct tgatacgacg
     4381 tatgaaaaat actatcaaca taatctgcat gcttaattat gttggcatac ttactttgat
     4441 tatgttcatt ccacttttct gtatgtcctt ggaacggtgt aattacagca aactttaacg
     4501 aatcatatgt tcgttgtaat tcaataacaa cttctgcagt ccataattct atacccattt
     4561 gcccttgtat taacacccat tctaatcctt catccaacag ttgttcaatt ttatgtttta
     4621 taaattgttt taaataatgt acttcaggtg cgtcatcttt aaaaatgttt aattcgaatg
     4681 atttgtaacc tgttacataa actgttttaa ccatcagtac cctcttttgt tacgccatct
     4741 aatatatttt gtaaatcata ttgaacactt ttaagctttt ctataaataa ctttcgactt
     4801 gttcgcttaa aatgacattc gacagaaagt tgttcaatgt tagcaatcag tagctcaaat
     4861 ttccttgaat tcatataagg tacttctata ataaattcac gatgtaattt gatctcattg
     4921 agtatgctat caatatgttc agtatatggc tttacagttt ggtaaaaatc atgatcttgt
     4981 tgttgtgatt tcacattttc aaaattttgt tgcatgttgt taacttcata aattagtgat
     5041 tcgactacat cattcatgag gacgcctcct acatttttta atttatcaca atatactgta
     5101 ttcgtcatgt tttaacactc tatataattt gatttaacta ttttttcaaa tgtgttatct
     5161 gttaaatcaa gtaaatctaa aaacttccta tataaataca aaattttatc gtgtatgttg
     5221 ttatacgatg aaaatacttt taatctaata aaatcattta aatcaaatac acctctgctg
     5281 attaacaaca catacttgta cttgcctcaa aaataaaaat tactaatcat gatttgactt
     5341 ttataacaaa attcaaaaat attgtaatga gtattcattt ttataagcaa aacttttcaa
     5401 ttaattaaaa ttgtgtgtaa aattccaaca ttaacggtaa atgtattata atattattgt
     5461 gttaatgctg aaatcacgtt aaaataagct ttggtgtatt gtgtctttcg catttatact
     5521 aatttaagtt tggtgtttca ctataatgtt gtgtataata aacaacgttt actaccaaac
     5581 ttgtgtggtg aaatttatga attatccaaa tggtaaacca tatcgtaaaa atagtgctat
     5641 agacggaggg aaaaagaccg ctgcctttag taatattgag tatggtggac gtggtatgtc
     5701 acttgaaaaa gatatcgaac attcaaatac gttttatctt aaaagcgaca ttgcagttat
     5761 tcacaaaaag cctacgccag tacaaatagt taatgtcaac tatcctaagc ggagtaaagc
     5821 tgtgattaac gaagcttatt ttcgtacacc ttcaacaact gattacaacg gcgtttatca
     5881 aggttattat attgattttg aagcaaagga aactaaaaac aagacgtcct ttcctttaaa
     5941 taatattcat gaccatcaag tcgaacatat gaaaaatgca tatcaacaaa aaggtattgt
     6001 gtttttaatg attcgtttta aaacgctaga tgaagtttat cttttaccct attcaaaatt
     6061 cgaagtattt tggaagagat ataaagataa tattaaaaag tctataacag ttgatgaaat
     6121 acgaaaaaat ggttaccata ttccttatca gtatcaacca agattagact atctaaaagc
     6181 agttgataag ttgatattag atgaaagtga ggaccgcgta tgacggaaaa caaaggatct
     6241 tctcagccta agaaaaacgg taataatggt gggaaatcca actcaaaaaa gaatagaaat
     6301 gtgaagagaa cgattattaa gattattggc ttcatgatta ttgcattttt cgttgttctt
     6361 ttactaggta tcttattgtt tgcttattat gcttggaaag cacctgcttt taccgaagct
     6421 aaattacaag atccgattcc tgcaaagata tatgacaaga acggagaact tgttaaaaca
     6481 ttagataatg gccaaagaca tgagcatgta aatttaaaag acgtgccgaa atcaatgaaa
     6541 gacgcagtac ttgcaactga agacaatcgt ttctacgaac atggcgcact tgattataaa
     6601 cgtttattcg gtgcaattgg taagaacttg actggtggat ttggttctga aggtgcctca
     6661 acattaacac aacaagttgt taaagatgca tttttatcac aacataaatc tattggacgt
     6721 aaagctcaag aagcatactt atcatatcgt ttagaacaag agtatagtaa agatgatatc
     6781 ttccaagtat atctaaacaa aatttactat tctgatggcg taacaggtat taaagctgct
     6841 gctaagtatt actttaataa agatttaaaa gatttaaact tagcggaaga agcttattta
     6901 gccggtttac ctcaggttcc aaacaactat aatatttatg atcatccaaa agctgctgaa
     6961 gatcgtaaaa acactgtttt atacttaatg cattatcata aacgcattac agataaacag
     7021 tgggaagatg ctaagaaaat cgatttaaaa gcgaacttag taaatcgtac tcctgaagaa
     7081 cgtcaaaaca ttgatacaaa tcaagattct gagtataatt catacgttaa ctttgtaaaa
     7141 tctgaattaa tgaataataa agcattcaaa gatgaaaatt taggtaatgt attacaaagt
     7201 ggtattaaaa tttatacaaa catggataaa gatgttcaaa aaacattaca aaatgatgtt
     7261 gataatggta gcttctacaa gaataaagac caacaagttg gtgcaacaat tcttgatagt
     7321 aaaactggtg gtttagttgc tatatctggt ggacgtgatt tcaaagacgt cgttaacaga
     7381 aaccaagcaa cagatcctca ccctactggt tcatctttaa aacctttctt agcgtatgga
     7441 cctgccattg aaaatatgaa atgggcaaca aaccatgcga ttcaagatga atcttcatat
     7501 caagttgatg gttctacatt tagaaactat gatacgaaga gtcacggtac tgtatctatt
     7561 tatgatgctt tacgacaaag tttcaatatc ccagctttaa aagcttggca atcagttaag
     7621 caaaatgctg gtaatgatgc acctaagaaa ttcgctgcca aacttggctt aaactacgaa
     7681 ggcgatattg gtccatctga agtacttggt ggttctgctt cagaattctc accaacacaa
     7741 ttagcatcag catttgctgc aatcgctaac ggtggtactt ataacaacgc gcattcaatt
     7801 caaaaagtag ttactcgtga tggtgaaaca atcgaatacg atcatactag ccataaagcg
     7861 atgagtgatt acactgcata catgttagct gagatgctaa aaggtacatt taaaccatat
     7921 ggttctgcat atggccatgg tgtatctgga gtaaatatgg gtgctaagac aggtactggt
     7981 acttacggtg ctgaaactta ttcacaatat aatttacctg ataatgcagc gaaagacgtg
     8041 tggattaacg gctttacacc tcaatacact atgtcagtgt ggatgggctt cagtaaagtt
     8101 aaacaatatg gtgaaaactc atttgtggga catagccaac aagaatatcc acagttctta
     8161 tatgaaaatg tgatgtcaaa aatttcatct agagatggcg aagactttaa acgtcctagc
     8221 tcagtaagtg gtagtatccc atcaatcaat gtttctggta gtcaagataa caacactaca
     8281 aatcgtagta cacacggtgg tagtgacaca tcagcaaaca gcagtggtac tgcacaatca
     8341 aataacaata ctagatctca acaatctaga aacagcggtg gattaacagg tatattcaac
     8401 taatccactc aacataaaat cctcagttat accatattta tggtgtagcc gaggattttt
     8461 ttaggttctt catcttttat ggtgggaagg taaaacttcc tgcttttttt ataagtattc
     8521 atacgctatt gctatattag tagaaaccgt aaaatcagtc attcacatac attttgaaac
     8581 atctaaatag tttttcatat tcacatcttt agctatgtaa aattattgaa tttgaataag
     8641 tggttgttcc catttttaaa attatgatta tattttctca tagtcatctt agacgctgtt
     8701 ttaaattgat atatttatta attccctgtg tacgttaatc agtgaaatat caacatgttt
     8761 gcaacatatt ttcacaatca tagatataag aaatccacct acgattcgaa atagatatct
     8821 taaagcatat ctcaagatat ccattatgat gtagatggac agtttttata ttaagacgtt
     8881 gtaatgacac gacacctaac ataattataa atatgtgtgc atgaattagt tttatatatt
     8941 ttcaacatta ggaaatgatc aatcttgtct atacctgctt attctttaac cttgtttgat
     9001 aactagcatt cattttaatc aattcagttt taaaggtctt catttgtcga tagcccataa
     9061 aatgatattt acgttcaatc atataatcat atctttcttt aaaattcatt ggcacaacag
     9121 gatattgatt taatttatca aaatcaatat tgccagttat gtcattaacc tgcttaaaat
     9181 aatcaataat tttactatgg tattcatcca agtagggctt tgcttcacta gaacctaatg
     9241 tcttattttt ggcaaataaa tcaatctctt gttctaaatt atcaaatacc tcttttgtaa
     9301 tatttgtcat gttatcacgc ttctttcaaa ctagctttat aacgtttttg tccttctcta
     9361 caatcttcta atagtggaca aatatcgcat ttaggttttc gggctaaaca gtggtatctt
     9421 ccaaagaaaa tgagttgatg atggctccta ttccatctat ctctaggtat gacagaacat
     9481 aaacggtctt ctacctgtct cacattatct ttccaacgat taatacctaa gcgtttagaa
     9541 acacgttcta catgcgtatc aacagctaat gaaggttcat caaatgctac actcatgact
     9601 acattagcag ttttacgtcc tacacctgct aaactttcta attccttatg tgtttgtggt
     9661 atttctccat taaattgatc aatcaaagat tgacaaagtt tcttaatatt cttagctttg
     9721 ttacgataca gaccgataga acgaatatca ttcataagtt cttcatcact gactgccaaa
     9781 taatcttcag gcgttttgta ttttttaaac agctcagttg ttactctatt tactagaacg
     9841 tctgtacatt gcgctgacaa taatacagca atagttaatt cgaacggatt atcatgtttt
     9901 aattcacatt ctgcatccgg aaacatattt gctataacat caatcatttc taatgctttt
     9961 ttcttactta ccatcaaggt tctccccgtt taaccaatca aatttaggta ccgttttaac
    10021 tgtgtgcgtc attttcggtt tattgaattt ttctcttatt tttctagaat cgtcaattgt
    10081 tttgacattg tttttcttcc aattaagtaa aatacgatct atatatttaa agctaagttt
    10141 attcaaacta ttcgcctcgt ctaatgccgc ttgtataatt gcagtatcgt gtttatcaac
    10201 atcaatccat tgatttaacg tttctatttc atatggagat aacggccttg caaatgtatc
    10261 ctctaaaact ctaaataatt gtttaaattt ttctttacta tttagctctt tcgtttccat
    10321 actttgttgc ttcaatatat gacttaattt ttcgaaaaaa ggatctagat tcatatattc
    10381 ggtaaatcta ccttcttcat ctttttgaac ttgtaattct agcaattcac gttgtatcaa
    10441 attttgaata accattgtaa tatcacgtgg ttgcatagtt gagcccttct gaagcaattc
    10501 aattgaaggc tgtttatttg atgtttcgga agcataaatc aatttaagca aaatgactaa
    10561 atcttgctca tctaaaccta agtcactgta atggtctaat aattctcttc gtatcactac
    10621 aggtcttgct tttaattgat atttatccat gttcgtgccc ccttttaaat ttaaccatta
    10681 cttaatagta taaatcattt tctttaattt tcgactaaaa tgcttaatat atttaactag
    10741 tagcaaacaa attttaaata acagtatttt ttaacactag attttaacaa tgaactttat
    10801 ggataatcag atgaactatt tattttccaa atatagttac taaacaaaag tttgttcatg
    10861 attaatataa gccgttatca atcttcattt tccaagaata aatcgcccag ctattaatat
    10921 aggctggacg attcttttta ttatcataat atatatttta agtaacgacc ctaaaactta
    10981 tggatataaa cggtttaata atcttgggaa tggcgctgtt tcacgaacgt gttcaacacc
    11041 agaaatccat gctactgtac gctctaaacc taaaccaaat ccacagtgtg gcacactacc
    11101 ataacgacgt aagtctaagt agtaactata tgcttcttcg tctaatccat gttctttaac
    11161 gcgttgttct aacaattcta agtcatccac acgttcagat ccaccaataa tttcaccgta
    11221 tccttcaggt gcaattaagt ctgcacataa tacagtttct tcattttcag gatttggttg
    11281 catatagaaa ggcttaattt tagttggata attagtaata aacaccggta aatcataatg
    11341 attagcaatg gctgtttcat gtggcgcacc aaaatcttcg ccccattcaa tatcatcaaa
    11401 gccttctgct tttaagaatt caattgcatc atcatatgaa attctaggga atggtgtcgc
    11461 aactttttca agttttgatg tatcacgctc taaaattttc aactctagtt tacaattttc
    11521 taaaactgat tttacaacat gtgttacata ttgttcttga atttctaaac tttcagcatg
    11581 atttgtgaaa gccatttctc cttcaatcat ccagaactcg atcaagtgtc tacgtgtttt
    11641 tgatttttca gctctgaaag ttggaccaaa tgaaaatact tttccgtgtg ccattgctgc
    11701 agcttctaag tataactgac cactttgaga taaaaacgca tcttgatcaa agtatttagt
    11761 atggaataat tcacttgtac cttctggcgc acttgctgtc aaaattggtg gatcaacctt
    11821 tgtaaatcca tctttgttga aaaattcata cgttgcacga ataacttcat ttctaatttt
    11881 cattacagca tgttgttttt tagaacgtaa ccataaatga cggtgatcca ttaagaattc
    11941 tgtaccatga tttttaggtg taatcggata gtcatgcgct tctgaaataa cttcaattga
    12001 tttcacttgc atttcgtatc ctaagtcaga acgattatct tctgtaattg tgcctgtaac
    12061 gtatagagat gattcttgag taatttcttt cgcaagtttg aatacctctt catcaacttc
    12121 tgatttaact actacgcctt gcataaagcc tgttccatca cgtaattgta aaaaggcgat
    12181 tttaccactt gaacgtttat ttgttaacca agcaccaatt gtaacgtctt ggtttaaatg
    12241 atctttcgct tgtttaatcg ttgttttcat aaccattctc ctatttattt tttcgttata
    12301 caatactcat tcattttaac aaaatccgct ttcaagttct agaactagac taaaagataa
    12361 cgtgtaaatg gtaatgattt acgcacagat ttgaacatta atttatatta agacaataca
    12421 atcatcagtt tcagacaaaa tatataataa agcctcaata ttattagtat gaaggtcgtt
    12481 ttttgtgcta acattcaagt ttaattataa aactcaaatt ttgacgacca aacaaaaatt
    12541 cttgtaacag aacatagcac atgtcatgtc acaagaattt tactttgtat ttcacttttt
    12601 ctttttttga atttgtctta ataattttcc aaactgttga atgtcgcctt ttttctgacg
    12661 ataattttca agtgtttgtt caaaaaagtt tttataatta ctgtttatga gtcgatcatc
    12721 aaatgaaact attatgccgc gatcattttc atttctaatt aatcttccaa gtccttgtct
    12781 aaaacgtgta actgcatcag gtaatacata ttccttgaaa gttgaagtga attcagaatc
    12841 cataagccaa tattttgcat tatgcttgtt cataaacggt aacttcgcta tcatcacaca
    12901 tttaatacca tttgcttgaa aatcaaaacc ttcaaaaaat gttgacgtac caagcagtat
    12961 ggccttatca aaattattaa actgttgtac tattttataa ttttggttct gctgttgtgt
    13021 taatacaaca taatcttcaa attctggcaa ttcatttagc atatcttgta ccatatgcat
    13081 cattttataa ctcgtaaata agactaaaca ttttgatgac gttatagtcg tatattcaat
    13141 aatatagctt acaatcgatg ctacatactc atctatattt ttatattgat aagatgctac
    13201 atcactcggt ataaatacac ttgtattttt tgcactttgt aacgacgtgt taacttcaaa
    13261 tgtattaaag tgaacatctt tgttgaataa ctgtttaaaa gcttcaaacg aatgattaaa
    13321 ttttaaagta ccagatataa aaatgagcga tttaaatttt tccaatactt gtttcgttaa
    13381 tacatctttt acagcgtaat ctttcacata taaacgtatt gttgattttt gagataaatt
    13441 tttaatcgaa atgaaactag tatgtccagc ttttaaactt tgttcgatat ttttaaattt
    13501 atcttttaaa tacaacaact gtttccgtaa tgatttaact gttttatgac taatgccatt
    13561 gaatatttct agcgttttat ttaacttatc gataatcgca tgtaaatcct tcaaaatgtc
    13621 ttttgtttca aaagtaaata cattatggaa gcgatgaata tcatcatcat aaacatcaga
    13681 atcattgata atcgtaaata tcgttgagaa caattgctca tttaactcat gaatctcatt
    13741 catactagcc ttcaagccaa aaatatcaat tggtgcaata tctaattttt ccaaaattcg
    13801 ctgcttttcc agttgatcaa ttgcctttaa caatttttca ttttcgtttt taccaatcaa
    13861 accaagctga tatttaatat cagcataact caactcattt gtcacttgat ttaaggcata
    13921 gtctggtaag cgatgtgctt catccactat acaatcatca aacaattgat atattgaatt
    13981 ttcaacatca gaatgaatta aatgtgcatg atttgtaata ccaatttgaa tgttctgtgc
    14041 atttcgctta ataaaattat aataatgaac atcgtgacgt gccggtacat atgtttcaat
    14101 tttctggtca aaatacatct tttgaccacc ttttaaattt aattcctgta tatctccgga
    14161 cggcgtctct gtaatccaaa tcagcaattg cattttcaag atattcactt cgtaattact
    14221 tgtgtcatct tttaaaattt gactaataag ccccaatgaa atgtaatcac ttttactttt
    14281 aatcaatagt gcattaattt taaaattcaa cgcttcattc attgctggaa tatctttttc
    14341 taacaattga ctttgcagta atttagtatt ggtagaaatc atgacatgct tcccagtttc
    14401 aatattatac atcaaggccg caagtaaata tgctaatgat ttaccactgc ctagtgatgc
    14461 ttcaatcatt gctttttcac tatgcatgag ctgatctaat atagtttccg ctaaatataa
    14521 ttgttgcggt cgatatgtta agccaagttg atctacagct ttgctatata aagacttcaa
    14581 gctgccatta taatttgttg tcggcttttt aaaatcaact tgcttacgat agataatctg
    14641 ttcgaacttt tcgtacgatt tatccaatgg ctttgcatca tattgcctaa ccatctcaaa
    14701 gaaaatatca tacaaatcgt atttcaactg tttacttaaa taatataatt gcttcaaagt
    14761 atctaacggt aacttttcaa atttttcaaa agctaatatc atcaatttag cagtagtagc
    14821 ggcatcttcg tcagctcgat gggcatttgc taaggtaata ccatgtgcct ctgctaattc
    14881 acttaattga tagcttttat ctgtaggaaa agctatttta aagatttcta gtgtatctat
    14941 aacttttttg ggacgatatt gaatattaca atctttaaat gcctttttaa taaaattcaa
    15001 atcaaaatct acattatgag ctacaaaaat gcaatctttt atcttatcgt agatttcttg
    15061 tgcaacttga ttaaaatatg gcgcttgttg tagcatattt tcttcaatgg atgttaacgc
    15121 ttgaatgaac ggcggaatct ctaaatttgt tctaatcata gaatgatatg tatcaataat
    15181 ttggttattg cgcacaaacg ttataccaat ttgaatgata tcgtcaaaat ctaattggtt
    15241 gcctgttgtt tccaaatcca caacggcata ggttgccata cccatagcta tctctccttg
    15301 ctttagtgtt aaaaatctat atctgcacta attaaacggt gtgattcacc cgcttcatct
    15361 ctaacaatta gatagccatc gtaatctaaa tcaattgctt gtcctttaaa ctgtttatca
    15421 ttttctgtaa atagcaacgt tctattccaa atattagaag ctgcagtata ttcttcacga
    15481 atttcagaaa aaggtaacgt taaaaattga ttatatcttt tttcaatttc ttgaagtaat
    15541 atctctaaaa attgatatct atctaattta tttttatcat gtaattgtat acttgttgct
    15601 ctatgtctaa tactttcatc aaagttttct agttgttgcg tcaaattaat acctatacca
    15661 catattattg cttctatacc atcattatta gcaaccattt cagttaagaa accacacact
    15721 ttaccattat caatatatat atcattcggc catttcactt tgacttcatc ttgactaaaa
    15781 tgttgaatcg catctcttat ccctaatgca ataaataaat taaatttaga tatcattgag
    15841 aatgcaacgt taggtcttaa cacgacagac atccaaagtc cttgcccttt tgaagaactc
    15901 caatgtctat taaatcgccc acgacctttc gtttgttcat cactcaagat aaaaaatgaa
    15961 gattgatttc caacaagtga ctttttcgca gcaagttgtg tagaatctat tgaatcgtat
    16021 acttcactaa aatcaaacaa agcagaactt tttgtatatt ggtctattat accttgatac
    16081 caaatatctg ggagctgttg taataaatgc cctttatgat ttactgaatc tattttacat
    16141 ccctctaact ttaattggtc aatcactttt tttactgcag tgcgtgaaat attaagtgat
    16201 tccgcaatgc tttgtccaga tatataattc ggtttatttt tatagagtaa ttgaagtaca
    16261 tcttgactat attttgacat gattatccac ccatttcaaa atttcagttt cttcgttgct
    16321 tactttacct gttacaatcg ctatctcaat ttgtcttagc acatctttta accacggacc
    16381 acttttggca tttaaatgtg ccataagtac accgccatta accatcatgt cttttctatt
    16441 atgcataggt aaacgatgta atgtttcatc aatcgtttga aggttaacga ttaatggttc
    16501 atgtccttga atatcatttg cctttaacac gtctgctgca accattacat ttttaatgag
    16561 atgcgtatca taatcataaa caaacatttt taattgttct tttgtaataa tacttggtaa
    16621 tgcattcata atttgaatat attgattgat atcttttact tgtcggttac ttagctttaa
    16681 aggcttcaat gagtaattaa tatcaaattt aactgatact atagcaatca acaattctaa
    16741 atcaattgct tcagttacat taatttgatt catatcaaga tgttcgaaat acggcatata
    16801 attaaatgct ttcagcgatt ttaaatgatt aaaactcttt tcaacattaa tacctcgcat
    16861 taatttagtt agttcaatca ctatacgctc aattgataaa aatttaatat ctgccatttg
    16921 tatacgcatc gcttcgaatg tttccgttgc aatatcaaat gataattgtg actggaacct
    16981 taaacatcga atcatacgta aagcatcttc ttggaatcgt tcctcagcta tacctacagt
    17041 tcttattatt cgattattaa tatcttgttg accatcaaaa taatcataca atttgtatgc
    17101 tgtatccatt gctatcgcat tcatcgtgaa atctcgtcgt tgcaaatctt cgtataaatc
    17161 acgaacaaat gtaacaccac ttggtctacg gtgatcgaca taatcttctt cagcccggaa
    17221 tgttgtcact tcataatttt catcattaaa aactacattt atcgtgccat gttctttacc
    17281 tacaggtatc gtatgactaa agatagattc tatttcatcc ggcgttgcac ttgttgtgat
    17341 atctatatca tgaatatttc ttcccatgac ataatctctt acagagccac ctacataata
    17401 tgcttcaaaa ccattgtctt gaatttgttc taatataggc cttgcctgtt caaataatga
    17461 tttatccata ttattactcg cctttacttt tgttatgctc atttagcatt ttttgataat
    17521 aatactcata ttgatctgta ataagttctg atccaaaacg ttcagcaata tctgctagca
    17581 tgtttttctg aagtttgttg tataacacct tatcttcaag taatcggata gcatagtcac
    17641 tcgctgaatc acaatcaccc acatctacga caaatccagt ttcaccatgt ttaataacct
    17701 ctttaattcc accggcattt gaaccaattg gaacgacgcc tgttttcata gcctcaagta
    17761 aagttagtcc aaagctttct ttttcactta ataataatac taagtcagat aattggtaaa
    17821 attcacttac gcaatcttgt ttccctaaaa ataaaacatc ctcttctacg tttaactctt
    17881 tcgtcaattg acgcattggc actaattcag gaccatctcc aagtaaaatt aatttactag
    17941 gtatcttttc acgtactttt gcaaatgttt ctataatagt atctatgcgt tttacttgtc
    18001 taaaattcga tacatgtatt aacacttttt catctggtgc tataccaaat tgtgatttta
    18061 atgctgtgtt atgtttagtt ggaaactcat tttcacgtac aaaattataa atcggtataa
    18121 tttctttgtt agtttcaata atttcatgtg tttcttgtgc taaagattta ctcacacttg
    18181 tcacaatatc acttttttca atgccaaatt taattgcacc ttggagtgaa tgatcatagc
    18241 ccaaaacagt aatatcagta ccgtgtagcg ttgtcataat ttttatatct ttacctgaca
    18301 tctcacgagc taaaatccca caaattgcat gaggtacagc atagtgcata tgcaacaaat
    18361 caagatcata ttctttaata acttcagcga ttttagtact taacgtaata tcatacggtg
    18421 gatactgaaa tactgcatat tgattcactt caacttgatg aaaaatcata ttcggtaatg
    18481 gttttcttat tctaaacggg atatttgaag tgataaaatg tacttcgtga cctcgctctg
    18541 ctaatttaat tcctaattct gtggcaataa ttccagaacc acccatggac gggtaacatg
    18601 ttatacctat cttcattcgc ttgcccatcc tttctttcta tttctctcta ttattctcga
    18661 tgcgtagata attgtttaaa tttaagttta tagtaatgtt gagtttataa tttcatatat
    18721 ctaaaaacag gtgttgtata tataatcatt catctagtta tacttacttt aaaaataata
    18781 taatttcatg cgatgcaatt cattgatgga tgtttttaat cttaatcaaa tccaaataaa
    18841 gcatatattt ttaaattcac tttctttcga atcgattttt atctcttgta ttaaactttt
    18901 ccattgtttc attaaagctc tctgtcatat ctattcccat tgaattcgct aaacataaca
    18961 acacaaataa attatcacct aattctgctt taatcgtatt tgcttcctct gaatctttct
    19021 tctttttttc accataggta tgatttattt cacgtgcaag ttcgcccact tcttcagtca
    19081 atctagctaa gttagctaat ggtgaaaaat atcctgtttt aaattgtcca atatattcat
    19141 caacttcacg ttgcatttct accattgatt tcatttctac gttctcctta tattgcattt
    19201 ctaatatagt atatatcaat ttgaagtctc atgcatgttt agtgcaatct actgactcta
    19261 gctaaactag atgacaagtt gtttatttta gaagtttaat aattcagttt atataaatgt
    19321 aatgcattcc taactaaatt aaatcaattg aaattgggat tataacttta tgatacgtac
    19381 cactacaata aaataatata gtgaataatc taccattaga aaaataagca caaaaaaact
    19441 agcaaccaca caaaaatgtg attagctagt taataagtgt ctaatttaag ttaattgtta
    19501 atctataaga ttaatcactt gaacgcgcaa tcaaaataat acgtacaagc tctgctacag
    19561 cgactgcagt tgctgcaaca taagtcattg ctgctgcaga taatacttta cgcgcatgct
    19621 tgtattcttt ttcatttaca atgttcaatg ccgtaatttg tttcatcgct cttgaactcg
    19681 catcaaactc aactggtaac gtaacaattg agaataatac cgctaatgac attaaaccag
    19741 caccaatcca taaagcagtt gaaccaaatg cactacctat cgctgttaag ataataccta
    19801 acatgatgat catataactt aatgaactcc ctaggtttgc aacaggtact aatgctgctc
    19861 tgaatcttaa gaaccaatat ccttggtgat cttgaatggc atgaccaact tcgtgggctg
    19921 caattgcagt tccagcaact gatggtctgt catagtttgc aggagatagt gaaacaactt
    19981 tctttttagg atcgtaatga tctgttaaga atccttcacc tttaacaact tcgacatcat
    20041 aaataccgtt tgcatgtaaa atttctaatg caacttcacg acccgtttta ccactagttg
    20101 atctaacttg tgaatatttc tcatagttag atttaacttt gtgttgtgcc cataaaggaa
    20161 gcaccattaa tattacgaaa taaattatca tagtaaaaat tgaagacaat aaactcactc
    20221 tcctttataa atattttact gtcatttgcc gtttttatca aatcatttac actttaataa
    20281 tttgtttaat tcaatataaa gcaaaagtcc aaaaacactt agacaacatg ataatacacc
    20341 aatttgccac acatgtgtag ttataaaatc ataatatgga aattgaaggt gaaaatagtc
    20401 aatataatca ttcaaaaaca cccaaatcat cgctacactg attccaatca tagaacgttt
    20461 aaacctagga tagaagtaaa ttgcctgaac agccattata ctgtgggaaa acattaatac
    20521 caaaccattt actgtaatat cac
//
LOCUS       DANJFZ010000040        20399 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.040, whole genome shotgun sequence.
ACCESSION   DANJFZ010000040 DANJFZ010000000
VERSION     DANJFZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.040"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            238..942
                     /locus_tag="PWN45_001288"
     CDS             238..942
                     /locus_tag="PWN45_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0623062.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1335..1871
                     /locus_tag="PWN45_001289"
     CDS             1335..1871
                     /locus_tag="PWN45_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623063.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(1984..3525)
                     /gene="guaA"
                     /locus_tag="PWN45_001290"
     CDS             complement(1984..3525)
                     /gene="guaA"
                     /locus_tag="PWN45_001290"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0623064.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3550..5016)
                     /gene="guaB"
                     /locus_tag="PWN45_001291"
     CDS             complement(3550..5016)
                     /gene="guaB"
                     /locus_tag="PWN45_001291"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL0623065.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5054..6322)
                     /locus_tag="PWN45_001292"
     CDS             complement(5054..6322)
                     /locus_tag="PWN45_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL0623066.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6322..6900)
                     /gene="xpt"
                     /locus_tag="PWN45_001293"
     CDS             complement(6322..6900)
                     /gene="xpt"
                     /locus_tag="PWN45_001293"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL0623067.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7131..7233)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7413..7820
                     /locus_tag="PWN45_001294"
     CDS             7413..7820
                     /locus_tag="PWN45_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDL0623068.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7963..8625
                     /locus_tag="PWN45_001295"
     CDS             7963..8625
                     /locus_tag="PWN45_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623069.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8743..9699
                     /locus_tag="PWN45_001296"
     CDS             8743..9699
                     /locus_tag="PWN45_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623070.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10490..10591)
                     /locus_tag="PWN45_001297"
     CDS             complement(10490..10591)
                     /locus_tag="PWN45_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623071.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10676..12064
                     /locus_tag="PWN45_001298"
     CDS             10676..12064
                     /locus_tag="PWN45_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL0623072.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12145..12900)
                     /locus_tag="PWN45_001299"
     CDS             complement(12145..12900)
                     /locus_tag="PWN45_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDL0623073.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13392..13961
                     /gene="ahpC"
                     /locus_tag="PWN45_001300"
     CDS             13392..13961
                     /gene="ahpC"
                     /locus_tag="PWN45_001300"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL0623074.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13977..15500
                     /gene="ahpF"
                     /locus_tag="PWN45_001301"
     CDS             13977..15500
                     /gene="ahpF"
                     /locus_tag="PWN45_001301"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL0623075.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIGQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15630..15872
                     /locus_tag="PWN45_001302"
                     /pseudo
     CDS             15630..15872
                     /locus_tag="PWN45_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15945..16571
                     /locus_tag="PWN45_001303"
     CDS             15945..16571
                     /locus_tag="PWN45_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDL0623076.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16826..17209
                     /locus_tag="PWN45_001304"
     CDS             16826..17209
                     /locus_tag="PWN45_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623077.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17275..17856)
                     /locus_tag="PWN45_001305"
     CDS             complement(17275..17856)
                     /locus_tag="PWN45_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0623078.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            18034..18243
                     /locus_tag="PWN45_001306"
     CDS             18034..18243
                     /locus_tag="PWN45_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623079.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18282..18533)
                     /locus_tag="PWN45_001307"
     CDS             complement(18282..18533)
                     /locus_tag="PWN45_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0623080.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            18833..19096
                     /locus_tag="PWN45_001308"
     CDS             18833..19096
                     /locus_tag="PWN45_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0623081.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(19234..19806)
                     /locus_tag="PWN45_001309"
     CDS             complement(19234..19806)
                     /locus_tag="PWN45_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0623082.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(19987..20355)
                     /locus_tag="PWN45_001310"
     CDS             complement(19987..20355)
                     /locus_tag="PWN45_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0623083.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
BASE COUNT         6859 a         3737 c         3111 g         6692 t
ORIGIN      
        1 attaggaatt cgcttataat tagatgttca atatagactt ttttacacat acatgaacta
       61 taaaaagcga ataaatagca ggtcattgcg aaatttgcta aatacaaaat tgatgagttc
      121 cacctaataa aaattgttgg ttttatagta acatctattt tcgtaccatt catgttaatt
      181 tcagtgctaa taatgctgat ataaagaaga ataaattgat taggagaaca ctatttaatg
      241 tctaaaaata tcaatcaagc aaatagtaaa ttaaatacgt caaacaaaaa actaaaacaa
      301 gcttattcaa aatccgacag taaaaacatg aaagttatat acatgcctca ttggctagag
      361 ttttattcaa ttgctataca tagcgatgtt acaagcaata aaaaaagata ttataaatca
      421 tatagtagag gaacagtggt atatgtaaaa ctagggagta atattggaag tgagttttct
      481 ggtaatcatt tttgtgtaat attagataac aaagacaata aaggaaaaga aacagttaca
      541 atagttccac tttcttccaa aggtaataaa aattatttga agttaaacga atcggtgcta
      601 aatctaacag caacagattt aaaaaaacag attatcgata tcagtagtaa agtacaagca
      661 ttagcccaaa aatatagaga aattgacatt aaacttagca cagaagataa aaagcttttg
      721 tctaacttaa atcaaaaagc taatgaactg tatagagtga taggtgttta ttccaagcac
      781 aaagggaaag atacatacgt taatatatca gcaattacaa caataagcaa aagacgtata
      841 agcaaaataa atgattctga tcctactgga acgataataa tttcagaaga tgacatgaat
      901 gaaatcgaaa agcaattaaa gataaagttc ttttctaatt gaattgtaat attacatctg
      961 atataattga tttacaaatt cccggtaacc attccggctt cattctcttg aagaatgaca
     1021 tattctcagc gttttagctg aaggtcagat gatacgtcat ctggcctctt tttttatata
     1081 aatgcccttt aagtgaaata tacaacaatc tgcaatcaat attagacatc acaaaacaca
     1141 caataaatta cacaatgtgt tataagccac atgtcactat tatcgcagct agctgcgtca
     1201 ctcaaaacga atacggaacg cgttggttat ttcaatcata atattactct gcaaatacac
     1261 agccctgtaa ccgagtaaat ggatagagat ttgaacaaat gaaaacaatc aactaatgga
     1321 aaggataaaa tattatgcac aacaataatg aattcaacaa aaaactgaaa gattttatag
     1381 gtagcgataa acggatggct ttagtaaagg gttatgttaa cgagtataaa ttaacaactg
     1441 ttttaagagc tttaaataat tccgattaca caaaagggac gatacacact cgttcaatag
     1501 gcaacctaaa acaaatagta gataatcgaa ttattcctaa aaatataact caaaacacaa
     1561 tcttcaaatt gagtaattta accttagagg ttaacctcta tgaaagaaac acaatttatc
     1621 acggagactt tgctatttac tatcctgtag aatctgctct aataatagaa acagatactc
     1681 ctaggctaat taatcatata aataacaacc cagtccaaaa aatatttata ataacaacaa
     1741 atgactggag ctttaataca cttgaattag aaaaaatagt agacgaaact ataatttatg
     1801 atttaaaaca agaagatcct aaacaatatg aaattttata taaaaataaa cacggaaaat
     1861 tgccttattg atgaattaaa acgcaaactt agaacacctt accccttttc cgtccttatt
     1921 gtaattttcc aactactcaa attaaaaacc acccgtgcaa acaggtggtt ttattatata
     1981 taattattcc cactcaattg tgcttggtgg ttttgatgta atgtcataga ctacgcggtt
     2041 gacgtgatct acttcgttta cgatacgact agaaatcttt tgtaagactt cccaatcgat
     2101 gcgtgcgaag tcacttgtca taccgtcgat agatgttact gcacgaatac ctactgtgtg
     2161 atcatacgta cggtagtctc ccataacacc tactgattga atgtttggta acactgtgaa
     2221 gtattgccaa atttctcttt caagaccttc ttctctaatc acttggcgta aaatcgcgtc
     2281 tgattctcta acgatttcta gtttatcttc agtaatttct ccaagtacac gaataccaag
     2341 accaggtcct gggaatggtt gtctccatac taaatgttct ggaataccta actcaatacc
     2401 taatttacgt acttcatctt taaacaatgt attgattggt tcgattaatt cgaattccat
     2461 gtcttctggt aatccaccaa cattgtggtg tgatttgatt gtttgtgctg ttttagtacc
     2521 tgattcgatg acgtctgtat atagtgttcc ttgcgcaagg aagtctacac ctttcagttt
     2581 tgatgcttca tcatcaaata cgtatacaaa ttcattacca atgattttac gtttttgttc
     2641 aggatctgaa acacctttta atttattcat aaagcgatct ttcgcattaa cacgaataat
     2701 attcatgttg aaaccttcac cgaattgctc cataaccatg tcgccttcac ctttacgaag
     2761 taagccatgg tctacaaaga tacatgttag ttgatcacct attgctttat gcaatagtac
     2821 agctacaaca gatgaatcta cgccgccact catcgcacat aatacacgac ggtctcctac
     2881 gcgttgacga atcttttcaa tttcgatttc gataaagttt tccattgtcc attgacctct
     2941 acaatcacaa acacgacgga caaaattatt taataaatca ttaccatatt ctgtatgacg
     3001 tacttctgga tggaattgaa caccataaat gcgacgtttc ttatcttcga ttgctgcata
     3061 gtctgtgctt gggctatcag cgataacttc aaagccttct ggaatttcaa taactttatc
     3121 agaatgactc atccaaacag tttgttctgc tggtaagcca gcgaataact catctgactt
     3181 cgcattaatg attgctttac cgtattcacg ttcattggca cgttcaactt taccacctaa
     3241 taatttagta gttaattgca tgccgtaaca aataccaagt actggaattc ctaaattata
     3301 tatttccgga tcaattgtaa atgaaccttc ttcataaact gaatttggac cacctgataa
     3361 gataatacct tttggattca ttttcttaat ttcttcaatt gaaatttcat gatcgtgtaa
     3421 ttcactataa acgcccattt cacgaattcg gcgtgtaatt aattggttgt attggctacc
     3481 aaagtctaag acaaggatta actcttgttc ttttgccatt tccatatttg tcgttctcct
     3541 ttatcttaat tagaatgagt agttcggtga ttctttcgta atttgaatat tatgtggatg
     3601 gctttctgct aaaccagcag gacccatacg tgtaaattgt gcttcttcgc gtaattctct
     3661 taaatcgtgt gaaccagtat aacccatacc agcacgcaca ccgcccatta attggtaaat
     3721 tgtatcttgt aacgcacctt tataagccgt acgtccttcg ataccttcag gaacaaattt
     3781 cttaggcgct ttgtcctctt ggaagtaacg gtcgtttgaa cctttttcca tcgcacctaa
     3841 agagcccata ccacggtata ctttatattg tctaccttgg aaaatttctg ttgcgcctgg
     3901 gctttcttca gtacctgcta ataagctacc taacataacc gcatgtccac cagcagctaa
     3961 tgctttaatg atatctcctg agaatttaat accaccatca gcaatgatag ctttaccatg
     4021 tttgcgtgct tcagttgcac aatcataaat tgctgtaatt tgtggtacac caacacctgc
     4081 tacaacacgc gtcgtacaaa ttgaacctgg gccaatacca actttaacaa tatctgcacc
     4141 cgcttcaaat aaatcttttg ttgcttctgc agttgctacg ttacctgcta ctaatgtgat
     4201 ttctgggtaa gtcttcttaa tatgtttcac ttgatcgata acacctttag agtgaccatg
     4261 tgctgtatcg ataactaaga catccacacc tgcttcgact aatttttgag cacgaatatc
     4321 agtatctttt gaaataccaa ttgctgcggc tacaagtaga cgaccatgtt catcttttgc
     4381 tgcattaggg aattcgataa ctttttcaat atctttaata gtaataagac cttctagacg
     4441 tccgtcttta actaatggta acttttcaat cttatgtttt tggagaattt tttctgcttc
     4501 ttcaagtgtt gtattcactg gagctgtaat taaattttct tgcgtcatta catctacaat
     4561 tttaatcgag aagtcttcaa taaaacgtaa gtcacggttt gttaaaatac ctactaagtt
     4621 gcgatcttct ttattatcaa caattggtac acctgaaata cggtatttac ccattaatgc
     4681 ttctgcttca taaacgcttt cttctggcgt taagaaaaat gggtttgaaa tgacaccatt
     4741 ttctgagcgt tttacttttt gaacttcgtc cgcttgttct tcaacgccca tatttttatg
     4801 aataacacct aaaccacctt gacgagccat agcaatcgcc attttagatt cagttacagt
     4861 atccatacca gcagaaataa ctggaatatt taatttaact ttgtctgata attgtacgct
     4921 taaatcaacg tctttcggta aaatatcaga ttgtgctgga attaataaca catcatcaaa
     4981 cgttaatgat tcttttgcaa atttactttc ccacattaaa aacagcctcc atttttcaaa
     5041 ttaattagtt atattatttc acattttctt ccgtttgttt atactttatt ccattaaaaa
     5101 agaaattaag aataattgca gagattgcgc ctaaaacaat tccgttttga gtcaaccatg
     5161 caaattgttc acctaaacct ttaaatgctt gtggtacagc gcttatacca gtacctaatc
     5221 ctactgatac agcgataatt aataaattgt tttgattttt aaaatcgata tgtcctaata
     5281 tactaacacc atatgccatt accatgccaa acatagctat catcgcaccg cctaacacag
     5341 gtagcggtat gatatttgct aatgcgccaa gcttaggtat acaaccacat ataagtaata
     5401 acacgaccat gccgtatata acattgtttt tcttagcgcc ggataaagaa acaagtccta
     5461 cattttgcga ataggctgta tacggaaatg aattgaatat agaacctaac actatcgcta
     5521 gaccttccgc agtataacct ttacgaaaat cttttctttc taacttctta ccggtaattt
     5581 cacttaacgc atgatagaca cctgtcgact caattaaact aacgatagct acaataaaga
     5641 acactaacgt cgatgtcaca tcaaagctaa atccagagaa tctaaacggc actgggatgc
     5701 ctaaccaacc ggcatgattg acttgattaa tatcgaccat cccaagtaag ccagcaccta
     5761 tcgttcctaa aacgagtcca attaatatgg caatactctt aataaatcca gttgtgaatc
     5821 tttgtaaaag aagaataatg attaatgtca ttaaacctaa caaaatgttc ttaacatctc
     5881 catagtcctt tgcaccttga cctccagcta agtaattcat tgctactggc attaaattga
     5941 taccaatgat agtaacaaca ctacccgtta ctactggtgg gaagaatttt acaagatgtg
     6001 aaaagaaagg cgcgatgata ataactaata tccctgataa aaatagcgaa ccataaagta
     6061 catctattcc tttcgtttga ccaattaaaa tcatgggcgc aacagccgtg aatgtacatc
     6121 caagaacgat tggtaatcct gttcctgtta ctttattggc ttgtaaaaat gtggcaaccc
     6181 cacacataaa tatatctact gtaactaagt aagcgatttg ttcaggtgta aacttcaaac
     6241 ttgtaccaac aatgattgga actaagatag cacctgcgta catagctaaa agatgttgaa
     6301 cacttaggat taaatttttc attattcttc tcccaccaat gtcactttgt ttccttctag
     6361 tgaagcaacc ttgcagagag aagaaactgt taaacctgct tcttctaaac gttgatgccc
     6421 attttggaaa ctcttttcaa caacaatacc aataccagct gtcttagcat tcgcttgctg
     6481 tgcgatatcg tataatccta atgaagcatc accatttgct aaaaagtcat cgatgataag
     6541 tacagtatct tcttctgata aaaactcttt tgaaacaatg accgtacttg ttttattttt
     6601 agtaaatgaa tgaatagatg tttcataata accatccgtc aaagtgctag gttttgcttt
     6661 tttcgcaaat aaacatggca catcaaaatg cagtgcagcc atgattgcag gtgcgatacc
     6721 ggaagcttca atggttaaga ttttagtaat ccctttatct ttaaattgct cgtaaaaagt
     6781 gcgaccaact tcattcatta actttgcatc aatttgatga tttaaaaatc catcgacttt
     6841 taaaatcttc tcatcaatga caacgccgtc ttcctttact ttttgtccta gtaactccac
     6901 tcttaaaacc tcctcagatt tgtgtgaaac attttgctct taaattggtg ctagatacaa
     6961 aaaaatcccc aaactaaata atagtttcag ggtttatgag tgaacgaaca tgcataacga
     7021 atttgtcatg caatcaatgt aaaagaagtt tcatcaaata aactgtgacc atcatataaa
     7081 atgatataaa tcacccacca tggttacaat ttaatggctg aagctactcc tagtattgtg
     7141 ttgttactca tagtcatgtc gttcaaggca acatggtaga aacttctaaa gccatattct
     7201 ttagattata tgagtttatg taaattattt aacgataata gcaaattttc ggcatttttt
     7261 caataactgc ttaggtaatc ttttaatagt tttataaatg tacagagacg tttaatgcga
     7321 atgaagactt ataacttaat aatgtaaggg ttttacttta taataagaag ggtaaaagat
     7381 taaataaaca aatttttaaa ggggcgacaa ctatggcaga tattactgta gtaaatgaca
     7441 ctggcgaatt atacaacgta atcaatcaaa agaaatctga aggttattta gaatcagagc
     7501 taacaattat tagtaaaagt aaattacacc taaatgactt acacgactct gaaatctccc
     7561 ttatctcaac aagtggaaca tttagtgata gaatgactaa actgttaaca ggagaagatg
     7621 gtgaacatgc agttttatca cgctacaact tagcgcctga tgaattagaa aaatataaac
     7681 aacttatttt agacgataaa atgttagttg tagcggttcg tgacaaatca tcacatcaag
     7741 aagtgcatga aaataattca gcttatgaag agattgatat tacacatttt gctgaagcat
     7801 ctaaaggtcc caaagcttag ttatcacact gaattcaaaa tgagattact gtactgtcta
     7861 atttaagata gtacagtttt ttataccatt taaaagttta tgaacagtct tttacagcag
     7921 gcgattatgc tataatatta tttaaattgg ggaggtgttc ttatggaatt taaaaccatt
     7981 caatctgtca aagatccaca ttacaacgaa gcacttaaac tttacgatac taagttaaat
     8041 ttatctttaa ctgaagataa acgtatcatc aaacgctcat tagaaaacaa caaaaccgaa
     8101 aacgattatg tctttatcgt aggcttagaa aacgatgttg ttgtcagtct agcaactgca
     8161 cattatgaag ccacaaccaa ttcagcattt ttaatttatt tattcgcaaa agacctaccc
     8221 aaccatgatg agttaatgac gcaaacatta catcaaattg aacatgaact aaacttatta
     8281 tcaaaccttg ttcattctag aagtattaac tttttaatga tggaagtccc taaagaacct
     8341 gcagatttaa cacaagataa cggcctcatt attgaacacc gtagacagtt tttatacgaa
     8401 catcaatttg aaagacaaac tgaaattgat tacattcatc caactaatga tggtcacact
     8461 aaaaaagtgg acttattttt aaagaaaaat attaaactga caaaggatat ctacggtaca
     8521 agtgttaaat caaattatat tttgaagtat gtatttgcca atggtatcgc acgtgaaatt
     8581 atttatgctt tattagaaga aatggattta cgtaaaccat tttaaacaaa cttgaaatca
     8641 ctaacaataa cgtttaaact atgtatgaag caagactgac gtttcactgc ccgtagacaa
     8701 acgtcagtat acataaagta tatgcagaaa ggactttaaa gtatgttaac taaagaattt
     8761 gcacaacgcg tagaactaag tgagaagcaa gtacgtaaaa ttgttcaaca tttagaagaa
     8821 cgtggttacc aactaagcaa gactgaatat cgtggtcgtg aagcaactga tttcaaagaa
     8881 gaagatatcg agcttttcaa agacattgcc gacaaagtaa aacaaacaaa tagttatgat
     8941 ctagcgtttg atgaattaga aaaagaaaaa gacttcctgc aagtcattgt caaaaacgat
     9001 gacaaaaact tacctactaa tcaaaatgtc gctcaactag tagaagattt acgcctagaa
     9061 atccagaaaa tgcgcgaaga acgtcaccta cttggtcaaa tgatgaatca agtacatcag
     9121 caacaacaag aattaaaaga acttcaaaat caacttacat ctaaaatcga ttcaaatagc
     9181 gaatccttaa aagccatcca aacatcacaa gaggctatcc aagaagcgca agcctctcaa
     9241 gcaaaagcat tagctgaatc aacaaataaa gttgaaaaga atgctgttac tgaagataaa
     9301 gctgacagta aagattcgaa ggttgctgga gtgaatacat caacagacgc taagacggat
     9361 actaaagcag aaaatgcagg cgatggcact gctactaaag tagataaaga ggatcaaatt
     9421 tctgcaactg aagcaattga aaaagccagc gttgagcagt ctaaaaatga caatgcagct
     9481 gagacttcaa ataaagaagc tacagtagat gcagaagcgc aacatgatgc tgaacaacaa
     9541 gtagcagaag cccatgcaga agcttctaaa caagctactt cgaacgattc attggaagcg
     9601 aaagcagaaa atgacagcac agcttcacag tcagaaatgt cagaaccaaa acctcaagaa
     9661 gagaaaaaag gtttcttcgc acgtttattc aacctgtaaa cttgtgattt gaacacaaat
     9721 ttaatactca attcatatat agctttgatt taaaaactga tttttataaa cctatagaac
     9781 tgtaatttac tttcaaattt aatactcaat tcataaaacc tgaaacaagc tgatatactc
     9841 cattcaatat ctactttgaa gagagcatat caaactaatt tgtttcaggt tttttaatta
     9901 ttcattatga ttaatattga attttgaata aatttttata gtatgttact tttaaatggg
     9961 atggcttctg tgtacttcta aatagattca tcttaataag tccattcaat ttaaatgttt
    10021 ggctgtgtta gtaactgacg aatcgcacaa gtgcacactc atattcacat ttactacaaa
    10081 cgcctttttg ctcaaacaaa aatatttgtt tacggtagct tttgaactac cacctgcaca
    10141 cacgaacact caaaagatgc taattttgat tcgacagaat tttttacaac gtgtttgttc
    10201 gaaacgtctt ttcaaggttg atcatttatt atcttttcat tcaataaata tgaggttagc
    10261 aatttttatc aaaccgccat agccacatct cgattcaagg ataaatttgt tttctcatat
    10321 cttatctctc atgacagtgc acgatatata tcttttccaa atattcatca cttttttcct
    10381 gttaaaatca tttctattat taatgcttta atgacaactt atttaattta ttgcatttaa
    10441 tgccctgctt cattttcaaa tattaacttt aatcgactag ctcatcacac tatttaccct
    10501 ttccacctat cactactgaa ttcaaaacga tttgtttaca aatcccctta tgccaacggt
    10561 cgaatttcat catgtttaca tttaattgca aatgccctct tgaaatattt taaaattaat
    10621 attattctta gcatactagt cggaattata ttcaaaaatt tgaggagtga cgttcatgaa
    10681 tgcttttctt acactgatta atattattgt attggtgatt ttcatcgtaa tattgcacat
    10741 gatggctcgc aaacatatat cttttgcgaa acgtgtgttt acagctttag ggattggtat
    10801 agtattcggt gtgttattgc atcttattta tggcacacat tcgaatgtta ttacatcaac
    10861 aagtgactgg ttcaacattg tcggacaagg ttacgttgca ttgttgcaaa tgattgttat
    10921 gcctttaatt ttcatttcta ttgtagctgc attcactaaa attcaaatcg gagaaaagtt
    10981 tgctaaaatt ggtagtctca tttttatctt tttaattggc actgtcacca ttgctgcgat
    11041 tgttggtgtt gtgtatgcct tggtgtttgg tttagatgca tcaacaatta accttggtaa
    11101 cgctgaacaa gcacgtggaa gcgaaattgc taaacaagcg aaagatttaa cagcacatac
    11161 gttaccacaa caaattttgg aattattgcc taaaaatcca ttcttagatt tcactggaca
    11221 acgtgcaact tccacgattg ctgtcgtgat atttgcttcg tttataggat ttgcttattt
    11281 acgcgtagca cgcaaacaac ctgatcatgg tgaattgctt aagcgtgcga ttgacgctat
    11341 ctattcacta gtaatggcga ttgtgacgtt cgtcttacgt ttaacgccat acggtgtatt
    11401 agcaattatg gccaacacat tatcgacaag tgactttggc gccatttgga ctttaggaaa
    11461 attcttaatc gcatcgtacg ctgcattaat tacgatgtat atcattcact taattatttt
    11521 aagcctgtta ggtattagcc caattcgcta tgttaaaaag actttggaag tattaatctt
    11581 tgcattcaca tcacgctcaa gcgcgggtgc cttaccattg aatgtacaaa cacaaacaag
    11641 acgtctaggc gtgccagaag gtattgccaa tttcgctgcg acatttggat tgtcgattgg
    11701 acaaaatggt tgtgcaggca tttaccctgc catgcttgca attatggtcg cacccgtcgc
    11761 taatgtagaa attgatttac aatttattgt aacgttaatc gctgtcgtga ttattagttc
    11821 attcggcgtt gctggcgtcg gtggcggggc tacatttgca tccatattgg tgttgtcaac
    11881 gttaaacttg ccagtcgctt tagccggcgt gctcatctcc gttgaaccat tgattgacat
    11941 gggtagaaca gcattaaacg tgaacgattc aatgctagct ggtacaggta ccgccaagtt
    12001 aactaaacat tgggataaag atactttcga ttcaaatgat aatgctgcat taacttcaca
    12061 ctaattcatt gcaagaaata cccgccccct ctagctatac ttatcatttt aagctagagg
    12121 ggggcgggtt attttggtat cttgctatcg ctgtattaag cctgattttt gcaattgttc
    12181 taacatatct aatcttgctt tgtttcctag gaacatctca atttgctgcg accatgtttc
    12241 tttgcgattc ccgtttgtac gttgatcata gtactcgctg attgtctggt cgtaatctgc
    12301 catttgtgca tactgtgttt ccttatcagc atgatactta ttatgatgga agacatggtc
    12361 aaatggtaag cgtggcttgg ctgcaccatt ttcgtcatct gcgggttccc ctactgccat
    12421 accaaatacc gggaagacat agtcaggtaa gtctaaaatt tctcgaacgc gttcaacatc
    12481 atttcttaat gatcctaaaa agacaatgcc ataccccata tcttcagcag ttaccgcaat
    12541 attttcggca actaatgctg catcgattgc acctactagc aaaccttccg ttgaaccata
    12601 tgcattttcc atatcagttt cagcatgttg atcaactaaa tgatgacgat aataatcaat
    12661 aacaaagacg aataaatagc cattttctac aacataaggt tgtccagaaa cttctcgtaa
    12721 attttcttta atcttctcat cgtcgatacc aataattgag tatgcttgca ggaaacttga
    12781 cgtcgaagcg ctttgtccag cttctaccaa tttcttaaca acgtcttcac ttaaaggttt
    12841 attcttaaat tttctaacag aatgatgctt tttcacaaga ttatatacat gttctgacac
    12901 gttacccact cctcgatact ttacaattaa tttaaaatct tcatattgca attgtacacg
    12961 tccaaacttt tttatagcaa ttatttgctt ataagttaat aattgaactt tttaaaatat
    13021 tgtctcatac tttaaaaatc cagtttctca ctcattacct tcatccatct cgaacaaata
    13081 ttctcacacc attatcaata aggttgactt agttgagaat acaaatcttc tttttttaca
    13141 ttttttacat taaaatcaag atattaaata tagacacaac cttttaaatc atcaactaca
    13201 aaatccatta tcaaagcact ttatattatc tttctttatt ttaaaagtaa tcattcactt
    13261 tacaaagtta tccagttgac tattctggat caaaatgtaa taatgctaac taagagatta
    13321 gaattattat aattaagcgc aatcaattga tagttctaat tcatacttaa attcttagga
    13381 ggaagatatt tatgtcatta attaacaaag aaatcttacc atttacagcg caagctttcg
    13441 atccaaaaaa agatcaattt aaagaagtta cacaagaaga tttaaaaggt tcttggagcg
    13501 tagtatgctt ctatcctgct gacttctcat tcgtttgtcc aactgaatta gaagacttac
    13561 aaaaccaata tgaagaatta caaaaattag gcgtaaatgt attctcagta tcaactgata
    13621 ctcacttcgt acacaaagca tggcatgacc attcagatgc aattagcaaa atcacttaca
    13681 ctatgattgg tgacccatca caaacaatca ctcgtaattt tgatgtatta gatgaagcta
    13741 ctggtttagc tcaacgtggt acattcatta tcgacccaga cggtgttgta caagcatctg
    13801 aaattaacgc tgacggaatt ggccgtgacg ctagtacatt agctcacaaa atcaaagcag
    13861 ctcaatatgt tcgtaaaaac cctggcgaag tatgcccagc taaatgggaa gaaggcgcta
    13921 aaacattgca acctggttta gatttagtag gtaaaatcta aggaggcatt acaaccatgc
    13981 ttaatgctga tttaaaacaa caacttaaac aactattaga actaatggag ggcaacgttg
    14041 aattcgttgc cagccttggt tcagatgata aatccaaaga acttaaagat ttgttgacag
    14101 aaattactga tatgtcacct agactatctc tttctgaaaa atctttaaaa cgtacaccaa
    14161 gtttctcagt caatcgtcct ggcgaagaaa caggtgtaac atttgcaggt attccattag
    14221 gtcacgagtt taactcactt gttttagcaa ttttacaggt tagtggtcgt gcacctaaag
    14281 aaaaacagtc aatcattggc caaattaaaa aattagaagg ttcattccat tttgaaacat
    14341 tcattagttt aacgtgtcaa aaatgtcctg atgtcgttca agcacttaac ttaatgagtg
    14401 tgatcaaccc taacatcacg cattctatga ttgatggtgc agtgttccgt gaagaatctg
    14461 aaaacatcat ggcagtccct gctgtctttt taaatggcga agaatttggc aatggtcgta
    14521 tgacaatcca agatattctt tcgaaactag gcagtacggc agatgcatct gagtttgaaa
    14581 ataaagaacc ttatgatgtc ttaatcgttg gtggtggtcc tgctagtggt agtgcagcga
    14641 tttacacagc acgtaaaggt ttacgtactg gtatagttgc tgatcgtatc ggtggccaag
    14701 ttaatgatac tgctggtatt gagaacttca ttactgttaa agaaacaact ggttctgaat
    14761 tttcttctaa cttagcagcg cacattgatc aatatgacat tgatgcaatg acaggtatac
    14821 gtgctacaga tatcgaaaag actgacgaag caattaaagt tacgttagaa aacggtgctg
    14881 tcttagaaag taaaacagtc attattgcta ctggtgcagg ttggcgtaag ctaaacattc
    14941 caggtgaaga gcaattgatt aataaaggtg ttgcattctg ccctcactgt gacggacctc
    15001 tatttgaaaa taaagacgta gcagttatcg gtggcggtaa ctctggggtt gaagcagcaa
    15061 ttgaccttgc tggtatcgtt aatcatgtta cattattcga attcgctagc gaattaaaag
    15121 cagacaacgt gttacaagat cgtttacgtt ctttatcaaa tgttgatatc aaaacaaatg
    15181 ccaaaactac tgaagttgtc ggagaagacc atgttacagg tatacgttac gaagacatga
    15241 acaccggcga agaacatcta cttaacttag atggtatctt tgttcaaatt ggtttacttc
    15301 caaacacatc atggttaaac gatgctgttg aattaaacga acgtggtgaa attgtgattg
    15361 atcgtaacaa taatacgaat gttcctggaa tatttgctgc tggcgatgtc acagatcaga
    15421 agaacaaaca aattatcatt tcaatgggcg ctggtgcaaa tgcagcatta aatgcctttg
    15481 actatattat cagaaactaa agtttcattc atttcactga cagtatctta catttgttaa
    15541 gatgctgtta tttttttatc ccgacataaa atcatgttat gataaactta atatatacaa
    15601 cgagatgtgc gataatgctt aaagtatgta cctctcgcaa acaaatcaat atgaaaaatg
    15661 atattgagaa agaaaagtat gtgaacgaaa ttattatgct tttacgtacg atacctacgt
    15721 ctaaattagt acaaccaatt atgcagttat ttatagataa agctatcctt aacaacgtcc
    15781 aaaatgatgc attattgata gaaaaagcaa ctcatgcacg taatcattta cgtctatggt
    15841 cattgcaagg tcatcgtgac attccaaact aattcaatgt tactctatta aacatgaata
    15901 catatttttt caattaaaga taaactaagg agttatatta cattatgaaa aagagattac
    15961 tactaagtac atttttagca tcgacattaa ttcttacagg ttgcgcgtcc gatcaatctg
    16021 ataacgaaga tcatcataca agtactggca tccatgcacc taaaagcgct aaaaaattag
    16081 aaacaaaaga tatctttaac tcagataaga aaaatagtga tattagtgat gcagagatga
    16141 agcaagctat agaaaagtat ttgtctgtaa atagcgatat acttgataac aaatatatta
    16201 tgcaacataa actagataaa caaattgaca gtcaaacaaa agtgactgaa aaacaagcag
    16261 aaacacttag ccacttatct aacttagctg ttaaaaacga tttgcatttc aagaaatttg
    16321 tcactgaaaa caatattcct aaagaatata agaaacccgt tgaattaatg atgaattact
    16381 tcaaagcttt aaatagtaca atcgccaatg ttgatgaaga tattgaaaag cttagctatc
    16441 aaccgcaaaa taaaatcaat gttgtcgacg tgccaacaaa atatgccggt gatgttaata
    16501 aaaagcaaca agataaaatt aaagatttct taaaatctaa aggtatcaag agtgatgtta
    16561 tcgataaata actgaaagcc atcattgcga aatgtaacac atcattacat gagttttaat
    16621 tcatgacaat tctttatctt taaaagtgac taatcaattt agctaaagtc atcatttggg
    16681 catcaagcag catattactg tttgatgcct ttttaattgc cttaaattct ctatctatgc
    16741 caaaattccc attagttgac gcatttgact atatacctaa catattccat acttatattg
    16801 actctataaa caaaggatga tcatgatgaa agttcgaatt acttttatta ttttagcaat
    16861 tttatcgacg attgtttgct tgttcttagc agcaatgcat ccaacaggac caaacacagt
    16921 aacttttgaa cagccatatt tattcaccct caacatcatt ataatggtat tagtcgcatt
    16981 accttcactt gttttagcta tatatgatta tatgagtttt agaatttctt ctgctatttt
    17041 acaatttcta ggggctatct cttggttctt tttatcattg atattatcgc tcacacaatt
    17101 tacacctttt acattagcgt catttataac ttcaattatt ttgttcacaa gcacaattat
    17161 cacattagcc attggtggta agtctgttga aaagaatgat tccccttaaa ttccaaatga
    17221 aaaaaaggtt ctgaaggccg ctataaaaca cagtttttca gaacctctat acttctattc
    17281 aatgatatat ggtttgcaat tttctacctt taaatccaca gcttctgccc ttgaaacttt
    17341 gttaaaataa accatcaaac aacgaatgac aacttgatgt gcaacaatga caatatcatc
    17401 tttttgtgta tcttcattga caacatgatt cataaaatgt tctacgcgtt gatatacatc
    17461 ttcataactt tctccttcag gcgctttttg tgaaaaacta tgacgaaagt ctttaaagtt
    17521 tggatcattg aaatatttat catatttcgg attcgcactg atttcatctt tatattcacc
    17581 ctcaaatacg ccaagtgaac gttctcttaa tagaggggta gtcgttgatg caatgtcata
    17641 tggaaaaata tgttcaaacg tttgctgtgt tcttaataag tctgaaacat atacatgttt
    17701 aatctgtttc tctttaaaat aatcacataa atcgtctgca ctttttgtgc cagtatccgt
    17761 taacggcaca tctaattgtc cacaaaaata agatcgaaaa tgtttattat cataattcga
    17821 ttttgattcg ccatgtctaa ctaaataaat cgtcataata ttactcctta ccttatgtat
    17881 ttcatatcta ccataacact ttgactacta attcgatatc aatcttaata ttctattcta
    17941 aaaaaagaat taattcatat tttaaattaa tgactctaaa aatagtattg tctttaatca
    18001 agtgatgtgt tactatatgg gtgtagttct taaatgacta cacagtcttt tatattcatg
    18061 aacataatac tcctggatgg cgcaacccaa cgtcatcctt tttttatttg tcaaatactc
    18121 gcttttttat ttccaaaatt tcctcatatt aatttattac tttttaatcc tatcttatct
    18181 acattttcaa atattataat acatattgac cattttcacg caaataaaaa agatgacaac
    18241 tgaatgccat ctttatttca atttccaatt gaattttttt attatttacg catagctctt
    18301 aaaattaacg ttacgattgc aattaagata attgaaccaa ttaatgctgg caagatgtaa
    18361 atacttccta attcaggacc ccattgtccg aatagtttgc cacctaccca tgatccaata
    18421 atacctgcga taatattgcc taaaatacca cctgggatat ctttacccat aatagcacca
    18481 gcagcccatc caattaagcc accgacaatt aacattccaa taaatccaaa cataattttc
    18541 agtctccttt ttctatttat tttgcgttat tctaagtagt accccttatt tacaattcta
    18601 aaacaaattc aaattatttt tatccaaata tttttaaaag tagtaattga atatcaattt
    18661 tattcaatgt agctatcgtt atttaaagtc tctgtaccga taatatcata tacatttaca
    18721 ttattttttc tgccgaattc atagcttgat tattttatgt tataggacta gaatatacac
    18781 atattattag agcatctttg aattttaaat caagaagcga ggttaatgaa caatgaatat
    18841 gcatatttta tataacttac gaactaaaca taatttagaa attgacgaat tagcacagca
    18901 attaaatgag aaatatggta ctaaatatga agcacatcaa atttgggaat gggagaatca
    18961 tcaccatgaa cctaaattta aagatgccat gcatttagct gacttctttg atgcaccata
    19021 tgaaatgttt ttagaaagta aggttaaaga atatcagaaa catttagaag aagtcgatat
    19081 tcgcatggat aaatagatgc aaataaaccc tcacaacacg tttggcatat atcctttcaa
    19141 atctatactg gatatattac attacgttgt gagggtattt tattaattaa tatgaattaa
    19201 gacattttac aagcgttaat gcaacgaatc tttttagtga tcttgctcac tctttaatac
    19261 tttaccgttc ttagcatcaa cagtaacttc ttgtttttta ttaccttttt tcaaatcgat
    19321 attgtaaaca agtttgccat catctttttc aagtgaccat tctttaatat caccatcaaa
    19381 ttctttttgt ccttctttaa tggctttttt gtaatctata gcatcgctat atttaaagtt
    19441 atcattttca ttcattgtat cttctttttc agtctttttg ttaatcactt ttttattttt
    19501 atcagcaaca agtacttctg actcttcacc agatttttgt tgcgtcactt tataagccca
    19561 ttcaccatta gaattttcaa atgaaattcc tttcaacttt tggcctttgt aagtttcttc
    19621 agcttttttc acagcatctt ctgggcttgt tttaacatct tttaaagcaa taacatcttt
    19681 tgttgtatta gtgtcttgat tagtatttga ctctgttgat tttgtttcat cttttggagt
    19741 atcattgcca catgcagtaa gcaccaccgc tgacattgat aacactgcta atgattttaa
    19801 tttcataata tcactctctc ttcctatttt tgaaactcat aacaaaagct tatatgctat
    19861 atagattgta ttaccccttg tttttaattt tattcataat tattacaaat atttttaaat
    19921 taatcgtcat gcgttacttt cgttcgtact ccttttataa atgaaccatg taatataagc
    19981 atgctattat cgactcagtt tgtctaatgc tttctttggt acttcttcct tttcaacttc
    20041 ttcaaaagtt tctacatgat gacctttatg tgtaattttc aagtatctat gcggtttaac
    20101 atcaaatgta gcagtataca ttaattgcgt ctctttccct tctttattaa aaacactttg
    20161 actataactt cggagttgat catccattct agtagacacc tctgtcgttt taacataaga
    20221 atcatccttc tttactaatg ggttaaattg atctgttata ccatgggagt ctattgtttt
    20281 taaaatgaat aaagcagcat aagcgccaat gatagtaagt acaagatatg ttattgtttt
    20341 taaaatcgtt ttcaaaagat cacctctgac aaaatataaa ataggatgta tctgaaaac
//
LOCUS       DANJFZ010000041        20178 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.041, whole genome shotgun sequence.
ACCESSION   DANJFZ010000041 DANJFZ010000000
VERSION     DANJFZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.041"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            214..705
                     /gene="lspA"
                     /locus_tag="PWN45_001311"
     CDS             214..705
                     /gene="lspA"
                     /locus_tag="PWN45_001311"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL0623084.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            705..1622
                     /locus_tag="PWN45_001312"
     CDS             705..1622
                     /locus_tag="PWN45_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0623085.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2021..2548
                     /gene="pyrR"
                     /locus_tag="PWN45_001313"
     CDS             2021..2548
                     /gene="pyrR"
                     /locus_tag="PWN45_001313"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL0623086.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2766..4073
                     /locus_tag="PWN45_001314"
     CDS             2766..4073
                     /locus_tag="PWN45_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL0623087.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4101..4982
                     /locus_tag="PWN45_001315"
     CDS             4101..4982
                     /locus_tag="PWN45_001315"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL0623088.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5000..6274
                     /locus_tag="PWN45_001316"
     CDS             5000..6274
                     /locus_tag="PWN45_001316"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL0623089.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6276..7376
                     /gene="carA"
                     /locus_tag="PWN45_001317"
     CDS             6276..7376
                     /gene="carA"
                     /locus_tag="PWN45_001317"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL0623090.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7369..10542
                     /gene="carB"
                     /locus_tag="PWN45_001318"
     CDS             7369..10542
                     /gene="carB"
                     /locus_tag="PWN45_001318"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL0623091.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10652..11344
                     /gene="pyrF"
                     /locus_tag="PWN45_001319"
     CDS             10652..11344
                     /gene="pyrF"
                     /locus_tag="PWN45_001319"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL0623092.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11344..11955
                     /locus_tag="PWN45_001320"
     CDS             11344..11955
                     /locus_tag="PWN45_001320"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL0623093.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            11985..12197
                     /locus_tag="PWN45_001321"
     CDS             11985..12197
                     /locus_tag="PWN45_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623094.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            12634..13035
                     /locus_tag="PWN45_001322"
     CDS             12634..13035
                     /locus_tag="PWN45_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0623095.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13298..14995)
                     /locus_tag="PWN45_001323"
     CDS             complement(13298..14995)
                     /locus_tag="PWN45_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDL0623096.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15270..15893
                     /gene="gmk"
                     /locus_tag="PWN45_001324"
     CDS             15270..15893
                     /gene="gmk"
                     /locus_tag="PWN45_001324"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL0623097.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15893..16111
                     /locus_tag="PWN45_001325"
     CDS             15893..16111
                     /locus_tag="PWN45_001325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0623098.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16327..17526
                     /gene="coaBC"
                     /locus_tag="PWN45_001326"
     CDS             16327..17526
                     /gene="coaBC"
                     /locus_tag="PWN45_001326"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL0623099.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17526..19934
                     /gene="priA"
                     /locus_tag="PWN45_001327"
     CDS             17526..19934
                     /gene="priA"
                     /locus_tag="PWN45_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL0623100.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7134 a         2930 c         3806 g         6308 t
ORIGIN      
        1 gatttatgtt ccagcctgct ttcctaattt ttaatgtcat cttaaatatg ataaatgaat
       61 aattaagttc atatttaatg tcaaaacata gtagtttatc aagtattgag ttagtaacat
      121 tagatttaat gtaatatgct tacttttttt attagcaggt gtaagctata atataaagag
      181 ttgtctcatg gacgattgat tggaggaacg aaaatgcaca aaaaatattt tattggcact
      241 tccattttaa tagcagtatt tgtcgttata tttgaccaag ttactaaata tattatagct
      301 actacaatga aaattggaga ttcatttgaa gtgataccgc actttttaaa cataacatca
      361 catcgaaata atggtgctgc atggggaata ttgagtggaa aaatgacatt tttctttatt
      421 attaccatta ttatattaat agccttagta tatttcttta ttaaagatgc tcaatataat
      481 ttgtttatgc aagttgctat tagtttactt tttgcaggtg cacttggaaa ctttattgat
      541 agaattttaa caggagaagt tgttgacttt attgatacaa atatttttgg ttatgatttt
      601 ccaatattta atatcgcaga ttcaagttta acaattggtg taatattaat tattattgcc
      661 ttattaaagg atacttccaa taaaaaggag aaggaggtta agtaatggag acttatgaat
      721 ttaacattac agataaagaa caaacaggta tgcgtgtaga taagttgctg cctgaattaa
      781 ataatgattg gtctcgtaac cagatacaag attggattaa agcaggttta gtcgttgcaa
      841 acgataaagt tgttaaatct aattataaag tgaaacttaa tgatcatata gttgtcactg
      901 aaaaagaagt ggttgaagct gatattctac ctgaaaattt aaatttagat atttattatg
      961 aagatgacga tgttgcagtt gtatataaac cgaaaggcat ggtagttcat ccatcaccag
     1021 ggcattatac caatacatta gttaatggtt taatgtatca aattaaaaat ttgtcaggta
     1081 ttaatggaga aattcgtcca ggtattgttc accgtataga tatggatact tctggtttat
     1141 taatggttgc taaaaatgat attgctcatc gtgggcttgt agaacaatta atggataaat
     1201 ctgttaaaag aaaatatatc gctttagttc acgggaatat tcctcatgat tacggtacaa
     1261 tcgatgcgcc aattggtaga aacaaaaatg atcgtcaatc tatggctgtt gttgatgatg
     1321 gtaaggaagc agtgacacat tttaacgtac tagaacattt taaagattat acgcttgttg
     1381 aatgtcaact tgaaacagga cgtacgcatc aaatccgtgt tcacatgaaa tatattggct
     1441 tcccattagt tggtgatcca aagtatggac cgaaaaagac attggatatt ggtggtcaag
     1501 ctctacatgc tggacttatt ggattcgaac atccagtaac aagtgaatat attgaaagac
     1561 atgctgaatt accacaagac tttgaagatt tattagatac aattcgaaaa agagatgcat
     1621 aattgtgtcg tgctataatt acgataacat tattatgtaa actataagtg tttatcgttt
     1681 tcgttttttt aaatgaaaac acattataaa atagattaag gttaacattt ttactgagta
     1741 tggttaattt ttcaatatag attgacaaag cgcctgatta gtattatcct tataacagtt
     1801 taagtacaac ataaagagtc tttaattgag cgtccagaga gacgtcaaag acatgaaaat
     1861 atagatatat actaaaatga tcataacact ttcacatatg caagaataag tagtaatact
     1921 acgattcttc tatgtaaata ggctgattgt atatactaaa cctcatgtcg atattaaaga
     1981 catgaggttt tttgtttcta aattttgaaa gggtgagaat atgtctgaac gtatcataat
     2041 ggatgatgcc gcaatacaac gtacagtgac gagaatcgct cacgaaattt tggagtataa
     2101 caaaggtact gataatttaa ttcttttagg tatcaaaaca agaggtgaat atttagcgaa
     2161 tcgtatacaa gataaaattc atcaaattga gcaacaacgt atacctactg gaacaattga
     2221 tattacatac tttagagatg atatagagca catgtcatca cttacgacaa aagacgcaat
     2281 agacatcgac acagatatta cagataaagt agtcatcatt attgacgatg tgctgtatac
     2341 tggtcgaacg gttcgtgctt cacttgatgc tattttgcta aatgctagac ctattaaaat
     2401 tggtttagct gctttggttg atcgaggaca tcgtgagtta ccaattcgag cagattttgt
     2461 tggtaaaaat atacctactt ctaaagagga aacggtaagt gtctatttag aagaaatgga
     2521 tcaaagaaat gcagttataa ttaaataata ccccttttaa ttcagtacga gagactgaaa
     2581 aaggtggggg agttgaatga taaataagtt ttcatatgct tgtttatcta ataataccct
     2641 catgttcgac aactataagt caatttctat ggacgaaaat gacttgtagc aacttgtcac
     2701 actttttgtc tctttacata tactgatgta aagagatttt tttattatcg gaggaaatga
     2761 atattatgca aaatgatgaa atgtttgaac gaacagtaaa acccgtacta gatgtaaatg
     2821 aaaaaccaca accagcgcaa tgggcatttt taagcttaca acatttattt gcgatgtttg
     2881 gcgcaacagt actagtacca ttcttaacag gactaccaat atcagcagcg ttactagctt
     2941 cgggaatcgg tacattactt tatatcttaa taacgaaggc gcaaatacca gcatacttgg
     3001 gatctagctt tgcatttatc acgccaatta tcacgggatt aagtacgcat agcttaggag
     3061 acatgcttgt agcattattc atgagtggtg ttatgtacgt catcatcggg attctaatca
     3121 aattaagtgg gacagcatgg ttaatgaaat tattaccacc agttgttgtt ggaccagtca
     3181 tcatggtaat tggtttaagc ttagcgccta ctgcagtcaa tatggcaatg tatgaaaatc
     3241 ctggagatat gaaaggttac aatatcagtt tcttaattgt tgcaatgata accttgcttg
     3301 taacaatagt cgttcaagga ttttttaaag gattcctatc tttaattcca gttcttgtag
     3361 gtattatcgt aggttatgtg gtagcgattt ttatggggat tgtgaaattt gatgcaatta
     3421 tgtcagcaaa atggatagat ttccctcata tttatctgcc atttaaagat tatgtacctt
     3481 catttcactt aggacttgta cttgtaatga ttccgattgt gttcgtaaca gtaagtgaac
     3541 atattgggca ccaaatggta ttgaataaaa tcgtaggtag aaacttcttt gaaaagccag
     3601 gacttgataa atcaatcatt ggtgatggtg tttctacaat gtttgccagt attattggtg
     3661 gaccaccaag tacaacatac ggtgaaaata tcggtgtatt agcgattacc agaatataca
     3721 gtatttacgt cattggtggt gcagcagtta tagcaattgt tttagcattc attggtaagt
     3781 tcactgcatt aatttcttct atacctacac cagttatggg aggagtatct atattacttt
     3841 tcggtattat tgcagcaagt ggcttaagaa tgttagttga aagcaaagta gattttgcga
     3901 acaatcgaaa tttagttata gcttctgtaa ttttagttgt aggtatcggt aatttagtat
     3961 ttaacttaaa agaaattggt atcaaccttc aaattgaggg gatggcatta gctgcacttt
     4021 caggaattat tttgaactta atcttaccta aagagaaaaa acaaaacaat taagatttac
     4081 aaattaagga gggcgctttt atgaatcatt tattatcaat ggaacattta tctacagatc
     4141 aaatatacaa acttatccaa aaggcaagtc aatttaaatc tggtgaacgt caactaccaa
     4201 actttgaagg gaaatatgtc gcaaatttat tctttgaaaa ttctactcga acaaaatgta
     4261 gttttgaaat ggcagaactt aagctagggt taaaaacgat tagctttgaa acatcaacat
     4321 catctgtttc aaaaggtgaa tctttatatg acacatgtaa aactttagaa agtattggct
     4381 gtgatttatt agtcattaga catccgttta ataactacta tgaaaaatta gcgaatatta
     4441 acatcccaat tgcgaatgct ggtgatggta gtggacaaca tccaacacaa agtttacttg
     4501 atttaatgac gatatatgaa gaatatggat attttgaagg cttgaatgta ttgatttgtg
     4561 gagacattaa aaattcacgt gtcgcacgta gtaattacca tagtttaaaa gcattaggtg
     4621 caaacgtaat gtttaatagc ccaaatgctt ggattgatga ttctttagaa gcaccttatg
     4681 taaatataga tgatgttata gaaacagtag atatagttat gttattaaga attcaacatg
     4741 aaaggcatgg gcttgcagaa gaaactagat ttgcagcaga tgattatcat caaaagcatg
     4801 gcttaaatga agtacgctat aacaaattac aagaacatgc tattgttatg catccggcac
     4861 ctgtgaatag aggagtagaa atacaaagcg atttagtaga agcttcaaaa tcaagaattt
     4921 ttaagcaaat ggaaaatggc gtttacttaa gaatggcagt cattgatgaa ttattaaaat
     4981 aggtaagggg acgaaaatga tgaaattaat taaaaacggt aaagtattac aaaatggcga
     5041 attacaacaa gcagatattt taattgatgg taaggtaatt aaacaaattg cacctgcaat
     5101 tgaaccaagc aatggtgttg acatcataga tgcgaaaggt cactttgtgt cacctggatt
     5161 tgtcgatgtt catgttcatt tacgtgaacc tggtggtgaa tataaagaga caattgaaac
     5221 tggtactaaa gctgctgcta gaggcggatt tacaactgta tgtccaatgc ctaacacaag
     5281 accggtacca gattctgtag aacattttga agctttacaa aaattaatcg atgacaatgc
     5341 tcaagtacgt gtattacctt atgcttcaat tacaacacgt caattaggta aagaattggt
     5401 tgatttccca gcactagtaa aagaaggtgc ctttgcgttt acagatgacg gtgtaggagt
     5461 acaaactgca agcatgatgt atgaaggcat gattgaagct gcaaaagtaa acaaagccat
     5521 cgtagcacac tgtgaagata attcattaat ctatggtggt gcaatgcatg aagggaaacg
     5581 cagtaaagag ttaggtatac caggtattcc aaacatttgt gaatctgttc aaatcgcaag
     5641 agatgtacta ttagctgaag cagcaggttg tcattatcat gtatgtcatg tttctactaa
     5701 agaaagtgtt agagtcattc gtgacgctaa acgcgcaggc attcatgtta cagctgaagt
     5761 tacaccacac catttattgt taacagaaga tgatattcct ggtaataatg ccatttataa
     5821 aatgaatcca ccattgagaa gtactgaaga tagagaggct ttgttagaag ggttactaga
     5881 cggtacaatt gactgtatcg caacagacca tgcaccacat gcacgtgatg aaaaagcaca
     5941 accaatggaa aaagcaccat tcggaattgt tggtagtgaa acagcattcc cattattata
     6001 tacgcatttt gtaaaaaatg gtgattggac attacaacaa ttagtagatt acttaacaat
     6061 taaaccatgt gagacattta atttagaata cggcacatta aaagaaaatg gttatgcaga
     6121 tttaacaatc attgatttag atagtgaaca agaaattaaa ggagaagatt tcttatcaaa
     6181 agcagataat acaccattta tcggctataa agtttatgga aatccgatct taacaatggt
     6241 tgaaggcgaa gttaaatttg agggggataa ataatatgca aagcaaacgt tatctagtgt
     6301 tagaagacgg ttctttttac gaaggctacc gtttaggatc tgataactta actgtaggag
     6361 aaattgtatt taatacagcg atgacaggtt atcaagaaac tatttcagat ccatcatata
     6421 caggtcagat cattactttt acgtatccat taatcggtaa ttatggtatc aatagagacg
     6481 attttgaatc attagtacct acattaaacg gtattgtagt gaaagaagcg agtgcgcatc
     6541 caagtaattt tagacagcaa aagacacttc atgacgtttt agaattgcat caaattccag
     6601 ggattgcagg tgttgataca agaagtatta cgcgtaaaat tcgacaacac ggtgtgttaa
     6661 aagctggttt tactgatcga aaagaagata ttgatcaact tgtcaaacat ttacaacaag
     6721 tagaattacc taaaaacgaa gtagaaatcg tttcgactaa aacaccgtat gtttcgacag
     6781 gtaaggatct aagtgtcgta cttgtagact ttggtaagaa gcaaaatatt gttcgagaat
     6841 taaacgtcag aggttgtaac gtcacagttg taccatatac aactactgcc gaagaaattt
     6901 tagcaatggc tccagatggc gttatgctat caaacggacc aggtaatcct gaagttgtag
     6961 aatgtgcgat tccaatgatt caaggaattt tagggaaaat tccgttcttt ggtatctgtc
     7021 taggacatca actttttgca ttatctcaag gagcaagctc atttaaaatg aagtttggtc
     7081 atcgtggtgc gaaccatcca gttaaaaatt tagagactgg aaaagttgat attacgagtc
     7141 aaaaccatgg atatgcaata gatatagatt cgttaaaaag tactgattta gaagttactc
     7201 atcttgcatt aaatgatggt actgtagaag gtttaaaaca taaaacatta ccagcatttt
     7261 ctgttcaata ccatcctgaa gcaaatccag gaccgtcaga ttcaaactat ctatttgatg
     7321 attttgtagc aatgatgact aattttaagg aaaaggagcg tcatatcaat gcctaaacgt
     7381 aatgatatca aaacaatttt agtaataggg tctgggccaa ttatcatagg tcaagcagct
     7441 gaatttgatt atgctggaac acaagcatgt ctagctttaa aagaagaggg atatcgagtt
     7501 attcttgtaa attcaaatcc agcgacaatc atgactgata aggaaattgc ggataaagta
     7561 tatatcgaac cgttaactca tgattttata gcgcgaatta tacgtaaaga gcaacctgac
     7621 gctttacttc caactttagg tggtcaaaca ggtttaaaca tggcgattca actacacgaa
     7681 agtggtgtgc ttcaagataa taacgtccaa ttattaggaa ctgagctaac atcaattcaa
     7741 caagcagaag accgtgaaat gtttagaaca ttaatgaatg atttaaacgt tcctgtacca
     7801 gagagtgaca ttgtaaatac agtagagcaa gcctttaaat tcaaagagca agtgggatac
     7861 ccgctaattg ttagaccggc atttacgatg ggtggtaccg gaggcggtat ttgtcataat
     7921 gatgaagaat tacatgaaat cgtctcaaat ggtcttcatt atagtccagc aacgcaatgt
     7981 ttattagaaa aatctatcgc aggttttaaa gaaatcgaat acgaagtaat gcgtgataaa
     8041 aacgataatg ccatcgttgt atgtaacatg gaaaatattg atccagttgg tattcataca
     8101 ggcgattcaa ttgttgtggc tcctagtcaa acattatcag atgttgagta tcaaatgtta
     8161 cgtgatgttt cattaaaagt tattcgagct ttaggtatcg aaggtggttg taatgttcaa
     8221 ttagcattag atccccattc attcgattat tatattatag aagtaaatcc gcgtgtatca
     8281 cgttcatcag cgttagcttc aaaagcaaca ggatatccta ttgcaaaatt agctgctaaa
     8341 atcgcggttg gtctaacatt agatgaaatg ttaaatccaa ttacaggaac atcttatgca
     8401 gcgtttgaac caactttaga ctatgtgatt tcaaaaatac caagatttcc ttttgataaa
     8461 tttgaaaaag gagaacgaga gcttggcaca caaatgaaag caacaggtga agttatggcc
     8521 attggtcgaa cttacgaaga atcattgtta aaagcaattc gatcacttga gtatggtgtg
     8581 catcacttag gattaccaaa tggtgaaagc ttcgatcttg attatattaa agaacgtatt
     8641 tcacaccaag atgatgaacg attatttttc atcggcgaag caattagaag aggcacaaca
     8701 ttagaagaaa ttcataatat gactcagatt gattacttct tcttacacaa gttccaaaac
     8761 attattgata ttgagcatca attaaaagag catcaaggtg atttagaata tcttaaatat
     8821 gcaaaagatt atggatttag tgataaaaca atagcgcatc gctttaatat gacggaagaa
     8881 gaagtatatc aattgcgtat ggaaaatgat attaaacctg tttacaagat ggttgatact
     8941 tgcgcagctg aatttgaatc ttcaacacca tattattatg gtacatacga aactgaaaat
     9001 gaatccatag ttactgacaa agaaaaaatc ttagtattag gctctggacc aattcgaatc
     9061 ggccaaggtg tagaatttga ctatgcgaca gttcacgccg tttgggcaat tcaaaaagca
     9121 gggtacgaag cgataattgt gaataacaat ccagaaacag tttcaacaga cttctcaatt
     9181 tctgacaaat tatactttga acctttaact gaagaagatg tgatgaatat cattaattta
     9241 gaaaaaccta aaggtgtcgt tgtacaattt ggaggacaaa cagcgattaa tttagcagac
     9301 aaattggcta aacatggtgt taaaatactt ggtacttcac tagaaaatct aaatcgtgct
     9361 gaagatagaa aagaatttga agcactatta agaaaaatta acgtgccaca gccacaaggg
     9421 aaaacagcta catcacctga ggaagcatta gcgaatgctg cagaaatcgg atatccggtt
     9481 gtagtaagac cttcttatgt attaggtggt cgcgcaatgg aaattgtaga caatgacaaa
     9541 gagttagaaa actatatgac ccaggctgta aaagcgagtc cggaacatcc ggtactagtc
     9601 gatagatatt taactggtaa agaaattgaa gttgatgcga tttgtgatgg agaaacggtc
     9661 attattccag gaatcatgga acatattgaa cgtgctggtg tgcatagtgg tgactcaatc
     9721 gctgtatatc caccacaaac tttgacagaa gacgagttag caacacttga ggactatact
     9781 ataaaattag ctaaaggttt aaacatcatt ggcttaatca acattcaatt cgttatagct
     9841 cacgatggtg tgtatgtttt agaagtaaat ccacgttcta gtagaacggt accattctta
     9901 agtaaaatta ctgatattcc aatggcacaa ttagctatgc gagcaatcat tggggaaaaa
     9961 ctaacagata tgggttatca agaaggggtt caaccatatg ctgagggtgt ctttgtgaaa
    10021 gcaccagtat ttagttttaa taaattgaaa aatgttgata ttactttagg acctgaaatg
    10081 aagtcaacag gtgaagtgat ggggaaagat actacattag aaaaggcgtt attcaaaggg
    10141 ttaacaggta gtggcgttga agttaaagat cacggtacag tattaatgac cgtcagtgac
    10201 aaagataaag aggaagttgt taaattggca caacgcttaa atgaagttgg ctataaaatt
    10261 ttagcaacgt ctggaacagc taataaatta gctgagtatg acatacctgc agaagtagta
    10321 ggcaaaattg gtggcgaaaa tgatttatta acacgtattc aaaatggtga tgttcaaatc
    10381 gttataaata caatgactaa aggtaaagaa gtagaaaggg atggcttcca aattagacgt
    10441 actacagttg aaaatggtat tccatgtttg acatctttag atacagctaa tgccttaacg
    10501 aatgtaattg aaagtatgac atttacaatg cgtcaaatgt aaatcaatca aactgtatcg
    10561 gtggggctgt aattaaccat ttacttaaag aagtttatat tacagcctca ttattttaat
    10621 gaatttctta atataaaggg agacatatat gatgaaagat ttaccaatta ttgcattaga
    10681 ttttgaatca aaagaaaaag taaatcaatt tttagattta tttgatgaat cattattcgt
    10741 aaaagtaggt atggaacttt tttatcaaga aggtcctcaa ttaattaatg agataaaaga
    10801 aagaggccat gatgtatttt tagatttaaa actgcatgat attcctaata cagttggtaa
    10861 ggcgatggaa ggactagcta aattgaatgt tgatctggta aatgttcatg ctgctggtgg
    10921 cgtaaaaatg atgtctgagg ccattaaagg attaagaaaa cataatcaag atacaaaaat
    10981 tattgcagta acacagctta cgtcaacaac agaagacatg ttacgacacg aacaaaatat
    11041 acaaacatcg attgaagagg ccgttttaaa ttatgccaag ttagcaaatg cagctggttt
    11101 agatggcgtt gtttgttcac ctcttgaaag tcgtatgttg actgaaaagt taggtacatc
    11161 atttttaaaa gtaacaccag gtattagacc taaaggtgca tctcaaaatg accaacaccg
    11221 tattacgaca ccggaagaag caagacagct tggttcgacg catattgtag tcggtagacc
    11281 gattacacaa agtgacaatc cagtcgaaag ttatcataaa attaaagaaa gttggttagt
    11341 ataatggcta aagaaattgc aaaatcatta ttagatattg aagctgtaac attatcacca
    11401 aatgatttat atacatggag ttcaggtatt aaatcaccga tttactgtga taaccgtgtt
    11461 acgttaggtt atcctttagt tcgaggcgca atccgcgatg gtttaattaa cttaattaaa
    11521 gaacactttc ctgaagtaga agttatttct ggtactgcaa cagctggtat tccacatgca
    11581 gcttttattg ctgaaaaatt aaaattacca atgaattatg ttcgttcatc aaataagagt
    11641 catggtaagc aaaatcaaat cgaaggtgct aaaagtgaag gtaaaaaagt agttgtgata
    11701 gaagatttaa tttcgacagg gggatcttca gtcacagcag ttgaagcctt aaaactagca
    11761 ggtgcagaag tattaggtgt tgtagctatc tttacttacg gtttgaaaaa agcagatgat
    11821 acatttagca atattcaact acctttttac actttaagtg attacaatga attaattgaa
    11881 gtagctgaaa atgaaggtaa aatttctagt gaagatatcc aaacattagt tgaatggaga
    11941 gacaacttag cataatatag acactagaag gaggaattca acaaatgaat gacaaaacat
    12001 ctaatgattt atatgggaag ataaaacatt gtaacgaatt tatcaatcat tcaaatgatt
    12061 ccaatctatc tagtagtcac gatgtcgacg aaagttcaac gaagcaaaaa catataaaaa
    12121 ataaaacaac tatagatcat aatgatgatt tatttaaaca tgtaaaggat atattacgta
    12181 aacaagaaca aatttaaaaa caaaaaagct atagtgttta atatcagggg ctgcttaacc
    12241 gcatccgaat attacaaaca ctatagcttt tattatataa aaaatttatt agcggataat
    12301 taactttatt aaaatccaac caattaagaa tacgagtaat aggactaata ctggaatcac
    12361 ataatgtaac ataacgtccc tcctttaact taattttaat tgtaatcaaa tttgacaata
    12421 agtcaaaaca ttaataccta tgataagtat catttattaa catatgtatc atatttttaa
    12481 tcttgcgtaa tttttatcgt taactatgtg atttaatcaa caggccaata agacgtttgt
    12541 cattcgtgca cataacggta cgacgcgctt ttgtcatttt aattatgtta agataatagt
    12601 agttatagaa gttcaatcta taggaggcat agcatggata ttccaaaaat cacgacattt
    12661 ttaatgttta ataaccaagc tgaagaagct gttaaactat acacaagctt atttgaagat
    12721 agtgagatta taacaatggc taagtatggt gaaaatggac ctggtgatcc cgggactgta
    12781 caacactcaa tatttacatt aaatggacaa gtattcatgg cgattgatgc taatagtggc
    12841 acagaattac caatgaatcc tgcgatttca ttatttgtta cagtaaaaga tactattgaa
    12901 atggaacgac tatttaatgg attaaaagat gaaggtgcca ttttaatgcc aaaaacgaat
    12961 atgccaccat acagagagtt tgcttgggtt caagataagt ttggagtaag ttttcaatta
    13021 gcattacctg agtaaaagga ttcgcacagc gttgaatgat aaagaaacac tttttcttat
    13081 gcatgcgttt tacctatgta tttctatttt aagtacacat tagcatttta gtttcactca
    13141 tttttaaaat cacaaagata atttgtaact taaatgatga tacgttattt aaaaacacga
    13201 tacttcgttt caatgaacgc attaaataat aaataaacac ctcaccataa gaaaggacta
    13261 ctttcttttg tgaggtgttt atttgtcgac aacttgatta tgattgtttc attttttgaa
    13321 taagttcata atcaggtgta gcatacaaag ttttttgatt gtcatatgtt acaaacccag
    13381 gctttgcacc tgatggttta tgcacatttt taattaatgt ataatcaaca ggtatttgtc
    13441 cagaattacc agcttttgaa aagtatcctg ctaacatagc cgcttccttg attgtcgtat
    13501 cacttggtgc atcattaaat ataacgacat gtgaaccagg aatatctttt gtgtgtaacc
    13561 atgtgtgagt ttttttagct tttttatttg ttaaataatc attttgcttg ttattcttac
    13621 caacatatat atcgtcgcca tcagttgata cataatgttg taattgaatc tgcgctttct
    13681 ttttcttagt ttgattttta cgctgtttca taaagccttg ttctgctaat tcatctctaa
    13741 tttcatcaat gtcatggaca gaaatatgat gtaattgttg ttcgattgtt gaaaaataat
    13801 ctatattgtc tttcgtcaat tgaatttgat gttgtaattc acgttctctc gttttcatac
    13861 gattatattg tttataataa tattgagcat ttgctgatgg ggattttgta ggatttaaag
    13921 gaatgacaac ttcttcattc gtataataat tcaatgccgt cacttcttta tcgccttgct
    13981 taattcgata tatattagca gtgatcaatt caccatataa ctgttcagta tctttatttt
    14041 tagactgctc atattcttca atcaacttcg ctaatttatt ttgatatttg tacaactgct
    14101 gttgaacaaa tcgaactaaa tcattcgcac gttgtttaac gcgttcacgt tcgccacgcg
    14161 catcataaaa acgatcaagc aaatcattta atgaatcgta tgtaactgta tcatcattaa
    14221 attgatttaa ctttataaaa tagaaatcct ctttacctgt ttcatgattt ttatgaaaaa
    14281 taggagtagg tggtaacttg gtttctgcca ttacttcgtc aaatgcttct ggtaatgttg
    14341 atgaagtaat aaattgacga cgactaacga tttcattcgt aattaaaggg ctaaatcctt
    14401 caaactgatt caataattgt ttagcaatat tacctgcgtt aaaatcgata tatttcaaca
    14461 cctctgcacc tgtaatatca tacggattta ttttgtgctg agtaggtggt gcttcataat
    14521 taaatcctgg cattactgta cgatagtgat tcgtatttgg tgttaagtgt ttaaatcctt
    14581 caattatttt gcgattttca tctactaaaa ttaagttact atgtttaccc ataatctcaa
    14641 ggatgacagt gcggtaaata gtatcgccaa tttcatcttt actctttata tcgatttcaa
    14701 tgcgacgatc attaccaatt tgcttaatcg attcgataat accaccttct aagtgttttc
    14761 taaaaacacg cgcaaacatg ggtggattaa atggattatc atatttttta gtagtcaatt
    14821 gtaatcttga aaagtttgga tggattgaca ataacaattg atggttttgt ctattttgac
    14881 gtacaaccat tagtatcgtg tcattatcag gttgattgat tttgtgaacg cgtcctgttg
    14941 ttaaaaattg tagagactca accattttct ttgtaaataa gccatcataa gccataattt
    15001 atcttccacc ttcttcatat cattcgtttc attttaacat gattgtaact tttcagtata
    15061 gttattgaac catacattat gccatacatt ttacattatc taaaagtttg caattcaatt
    15121 tatttaatta aaattaatcg tgcaatataa cgatattgtt agacttaata gaaattatgg
    15181 catgcaattt caaatatgct atacaatata aagaacaatg tgatatcata tttaaataat
    15241 agaagattag cttagagagg tcgtaaggca tggataatga aaaaggattg ttaatcgttt
    15301 tatcaggacc atctggagta ggtaaaggta ctgttagaaa acgaatattt gaagatccaa
    15361 gtacatcata taagtattct atttcaatga caacacgtca aatgcgtgaa ggtgaagttg
    15421 atggcgtaga ttactttttt aaaactaggg atgcgtttga agctttaatc aaagatgacc
    15481 aatttataga atatgctgaa tatgtaggca actattatgg tacaccagtt caatatgtta
    15541 aagatacaat ggacgaaggt catgatgtat ttttagaaat tgaagtagaa ggtgcaaagc
    15601 aagttagaaa gaaatttcca gatgcgctat ttattttctt agcacctcca agtttagaac
    15661 acttgagaga gcgattagta ggtagaggaa cagaatctga tgagaaaata caaagtcgta
    15721 ttaacgaagc gcgtaaagaa gttgaaatga tgaatttata cgattacgtt gtagttaatg
    15781 atgaagtaga acttgcgaag aatagaattc aatgtattgt agaagctgag cacttaaaaa
    15841 gagagcgcgt agaagctaag tatagaaaaa tgattttgga ggctaaaaaa taatgttaaa
    15901 tccaccttta aaccaattaa cgtcacaaat taaatcaaag tatttaattg caacaactgc
    15961 agcgaaaaga gcgcgtgaaa ttgatgaaca acctgaaact gaattattaa gtgaatatca
    16021 ttcatttaaa ccagttggta gagcgttaga agaaattgct gacggtaaaa ttcgccctgt
    16081 tatttcaagt gattattatg gtaaagaata gtttcgacca ttaaaaatat gtgaagctag
    16141 acgtacatca aatacgtcaa ggcatatcca aatcaaacgc acctatgtag gtgtgttttt
    16201 ttagtttatc caatgattga tgttataata atactaaatt tgtatctata aaaaagtaat
    16261 gagcatttgt gcgcatatga tgatgtaaag cgtaaatgtc atagtgatat ttaagggaga
    16321 ttgaatatga agaaaatatt attagccgtt acaggtggca ttgcggcata taaagcaatt
    16381 gatttgacaa gtaagttaac acaatctggg tatgaagttc gcgttatgtt aacgaatcac
    16441 gcacaaaaat ttgtgacacc attagcattt caagcaataa gtcgaaatgc tgtttataca
    16501 gatactttta tagaagaaaa tccttcagaa atacagcata ttgcattagg tgattgggca
    16561 gatgcaatca ttgttgcacc tgcaacggca aatacaattg caaaattgag tgtaggtatt
    16621 gctgatgatt tggtgacatc aacgttgcta gcaacagaga caccgaaatt tattgcgcct
    16681 gctatgaatg tgcatatgta tgaaaataaa cgtacgcagc aaaatattaa tattttaaaa
    16741 gaagatgggt atcattttat cgaaccagga agcggatttc tagcatgtgg ttatgttgct
    16801 aaaggacgta tggaagaacc gcttcaaatc gtttctgtaa ttgatgctca ttttcaaaat
    16861 agtaatcgtt tagctaatag ttcatttcaa gataaacgcg cattggttac agcaggacca
    16921 actattgaag ttatcgatcc agtcagattt gtatccaatc gttcttctgg aaaaatgggc
    16981 tatgcaatag ctgaagcatt gcgaaatcga ggagctatcg tgacgttagt tgctggtcct
    17041 acaacactag aggatccaaa agatattgaa gttattcatg ttcaaagtgc tgaagaaatg
    17101 tttgaacaag tgacaagccg atttgacgaa caagatattg ttgtaaaagc agcagccgta
    17161 tctgactata caccggttga tgtattagaa cataagatga aaaagcagga tggtgatttg
    17221 tcagtatctt ttaaacgtac taaagacatt cttaaatatt taggtgaaca taaaacatca
    17281 cagtatttaa taggctttgc agcagagact gaagatattg aaaattatgc acaacaaaaa
    17341 ttacgcaaga aaaatgcaga tgtgattatt tcaaataatg ttggggatat gtctatcgga
    17401 tttagttctg atgataatga attgacaatg cattttaaaa ataatgaaaa agtaaatatc
    17461 aagaaaggaa aaaaagtagt attagctgca caaattttag atgaactaga aactaggtgg
    17521 caataatgat agcgaaagtc atagtcgatg tcgcgtcgaa gagcgttgac tataaatttg
    17581 attatataat tcccgaacaa ctcgaatctg tcatccaacc tggtgtgcgt gtaattgtac
    17641 cttttggacc aagaacgatt caaggttatg taatggaagt aacagcagaa cctgatgcac
    17701 aacttgacgt ttcgaagtta aaaaaaatca tagaagtgaa agatatacaa ccagaattaa
    17761 catcagaatt aatagcttta agtgagtgga tgggttcaac tcatgtcatt aaacgtattt
    17821 ctatgctaga agtgatgctt ccgagtgcta ttaaagcgaa gtataaaaaa gcatttaaga
    17881 tgaaagatga catagagcta ccttcagctt tattacaaaa atttgataag catggttact
    17941 attattataa agatgcgcaa aaaaataatg atattcaatt gcttatgaag ttgttaaaag
    18001 atgatatcgt tgaagaaaaa acgattctca cacaaaatat aactaaaaaa accaagcgtg
    18061 ctgttcgtgt cattgaaggg tatcatcctg atgaagtatt agctaagttg gagaaagtta
    18121 ttaaacaata cgatttgtat gcttacttgt ctgaagaaca acataaaaca atatttttaa
    18181 ctgatattga ggatatgggc ttttcaaaat ccagtttaga tggacttatc aaaaaaggtt
    18241 atgttgaaaa atatgacgcg gttgttgaaa gagacccatt taaagatcgt gttttcgaac
    18301 aagaatcaaa acagcaatta acagaagacc aatataaagc atatgaagcg attaaagcta
    18361 aaattgtaag ccaagagcaa gaaacatttt tacttcatgg tgtgacggga tcaggtaaaa
    18421 cagaagtata tttacaaacg atagaagatg ttttaagcca aggaaaacag gcgatgatgt
    18481 tagttcctga aatcgctcta acaccgcaaa tggttttacg cttcaaacgt cgatttggtg
    18541 atgacgttgc tgtattacat tctggcttat ctaatgggga acgttatgat gaatggcaaa
    18601 aaattaggga tggtcgtgcg agagtaagtg ttggtgcaag gtcaagtgtg ttcgcacctt
    18661 tcaaaaattt agggttaatc atcattgatg aagaacatga atctacatat aaacaagaag
    18721 attatccgag atatcacgct agagaaattg cccaatggcg aagtgaatat catcactgtc
    18781 cagtcatttt aggaagtgca acaccatgtc ttgaaagtta tgcacgagct gaaaaaggcg
    18841 tttatcattt gctatcatta ccaaacagag tgaaccaaca agctttacct gaaattgata
    18901 tagtagacat gcgtgaagaa ttgagtgaag gtaatcggtc aatgttttca aaagatttac
    18961 gtgaagccat acaattaaga ttagatcgac aggaacaagt tgttttattt ttaaatcgac
    19021 gtggttatgc atcgtttatg ttatgtcggg attgtggata tgtaccgcaa tgtccaaact
    19081 gtgatatttc attaacgtat cataaaacga cagacttatt aaaatgtcac tattgtggtt
    19141 accaagagac gccaccgaat caatgtccaa attgtgagag tgaacacatt cgacaagtag
    19201 gtactggtac tcagaaagtt gaagaactat tgcaacaaga atttgaagat gcgcgcataa
    19261 ttaggatgga tgtagataca acctcaaaga aaggtgcaca tgaaaagtta ttgactgaat
    19321 tcgaaaaagg taacggtgac attttactag gtactcagat gattgcgaaa ggattagatt
    19381 atccaaatat tactttagtt ggtgtgctga atgcagatac aatgttaaat ttacctgatt
    19441 ttcgggcgag cgaacgtact tatcaactat taacgcaagt ggctggtaga gctggtcgtc
    19501 atgaaaaggc aggtcaagtc atcattcaaa cgtataatcc agatcattat tcaatattgg
    19561 atgttcaaaa aaatgattat ttaacatttt atcgtcagga aatggaatat cgtaaattag
    19621 gaaagtatcc accgtattat tatttgatta atttcacaat ctcacataaa gaaatgaaga
    19681 aggttatggt agcatcgcag catgttcata aaattttatt acagcattta acagaaaaag
    19741 cgcttgtact aggtccatct ccggcagcac ttgcgagaat caacaatgaa tttagattcc
    19801 aaattttagt gaaatataaa agtgaacctg gattattaca agccattcag tttttagatg
    19861 actattacca tgaaaaattt ataaaagaaa aattagcatt gaagattgat attgatccac
    19921 agatgatgat gtaacattac taattattag aaacaagttc agtatgtacg agtatttgaa
    19981 ccagtgtttg aatatttact ttaagtacag aaaaagggca gaatatacaa ctgttaacta
    20041 tttaaattag cagtttatat tctgtctttt tatatggctt tataacttac gtgattttgg
    20101 tttgataagg aatttattag tattttcatt tacgaattct gattttaatg caagcgccat
    20161 acttactgat gaataaaa
//
LOCUS       DANJFZ010000042        19999 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.042, whole genome shotgun sequence.
ACCESSION   DANJFZ010000042 DANJFZ010000000
VERSION     DANJFZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.042"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(259..2868)
                     /gene="adhE"
                     /locus_tag="PWN45_001328"
                     /gene_synonym="adhC"
     CDS             complement(259..2868)
                     /gene="adhE"
                     /locus_tag="PWN45_001328"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL0623101.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(3318..3802)
                     /gene="tnpA"
                     /locus_tag="PWN45_001329"
                     /pseudo
     CDS             complement(3318..3802)
                     /gene="tnpA"
                     /locus_tag="PWN45_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4106..4360)
                     /locus_tag="PWN45_001330"
     CDS             complement(4106..4360)
                     /locus_tag="PWN45_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623102.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
     gene            complement(4516..5424)
                     /locus_tag="PWN45_001331"
                     /pseudo
     CDS             complement(4516..5424)
                     /locus_tag="PWN45_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(5649..5861)
                     /locus_tag="PWN45_001332"
                     /pseudo
     CDS             complement(5649..5861)
                     /locus_tag="PWN45_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(5936..7471)
                     /locus_tag="PWN45_001333"
     CDS             complement(5936..7471)
                     /locus_tag="PWN45_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL0623103.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(7522..9066)
                     /locus_tag="PWN45_001334"
     CDS             complement(7522..9066)
                     /locus_tag="PWN45_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0623104.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            9295..10251
                     /locus_tag="PWN45_001335"
     CDS             9295..10251
                     /locus_tag="PWN45_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0623105.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            10465..11238
                     /gene="phnC"
                     /locus_tag="PWN45_001336"
     CDS             10465..11238
                     /gene="phnC"
                     /locus_tag="PWN45_001336"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL0623106.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            11240..12040
                     /gene="phnE"
                     /locus_tag="PWN45_001337"
     CDS             11240..12040
                     /gene="phnE"
                     /locus_tag="PWN45_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL0623107.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            12037..12852
                     /gene="phnE"
                     /locus_tag="PWN45_001338"
     CDS             12037..12852
                     /gene="phnE"
                     /locus_tag="PWN45_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL0623108.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(12983..14161)
                     /gene="deoB"
                     /locus_tag="PWN45_001339"
     CDS             complement(12983..14161)
                     /gene="deoB"
                     /locus_tag="PWN45_001339"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL0623109.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(14189..14851)
                     /gene="deoC"
                     /locus_tag="PWN45_001340"
     CDS             complement(14189..14851)
                     /gene="deoC"
                     /locus_tag="PWN45_001340"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL0623110.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            complement(14932..16284)
                     /gene="tet(38)"
                     /locus_tag="PWN45_001341"
     CDS             complement(14932..16284)
                     /gene="tet(38)"
                     /locus_tag="PWN45_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDL0623111.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            complement(16291..16998)
                     /gene="deoD"
                     /locus_tag="PWN45_001342"
     CDS             complement(16291..16998)
                     /gene="deoD"
                     /locus_tag="PWN45_001342"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0623112.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            17234..17989
                     /locus_tag="PWN45_001343"
     CDS             17234..17989
                     /locus_tag="PWN45_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0623113.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(18181..18906)
                     /gene="sasD"
                     /locus_tag="PWN45_001344"
     CDS             complement(18181..18906)
                     /gene="sasD"
                     /locus_tag="PWN45_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDL0623114.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(19274..19873)
                     /locus_tag="PWN45_001345"
     CDS             complement(19274..19873)
                     /locus_tag="PWN45_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL0623115.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT         6344 a         3666 c         3060 g         6929 t
ORIGIN      
        1 ttttaaaccg cattattttc gcctttttat ttatatgaaa ttaaaaataa ttatactcta
       61 aagtagtaat agtttgtgaa atgagtatca atcttaatat tttctttatt taaaaaaatg
      121 attgtctagt ttgtatctct ctgaagattt ggcaataaat aaaagccgat aaccgtataa
      181 tgattatcga cttaaagttt atgtggcatt ttttactttt gtaatttcag gtgagttaga
      241 tgattattat cagatagatt attgcttata atcatatgat gtttgaatga tatctttgat
      301 ttcactgatt agtgcttctt taggattagc agttgtacat tgatcttcaa atgcgagctc
      361 tgccattcta tcaattgact catttaattc ttcttcagac acaccttgtg atttcaaatt
      421 catttcaatt ccgactgatt gacctaattc gtagacagct ttagctaatg attctacgag
      481 tgcttctgtc gtattacctt ttaatcctaa gaatttggca atatctgcat aatctgtatc
      541 tgctctgaag aactcatatt tagggaataa tgcatgtttt tgcgggtctt tggcattata
      601 acggataata tgcggtagta atatcgcatt cgctctacca tgcggaatac catattcgcc
      661 accaatttta tgcgcaattg agtgtgcaat gcctaagaat gcatttgcaa atgccatacc
      721 agccaaagtt gatgcgttat gcattttctc tcttgaaact ttatcaccct tttcaacaga
      781 tgattttaaa tattcgaacg tcaatttaat cgcttgtaga ctcaaacctc ttgtgtagtc
      841 tgaagccatt acagatacat atgattccat tgcatgcgtt agtacatcca ttcctgtatc
      901 tgctgtaacg ctttttggca cactcatcac aaattgaggg tcaataattg caacgtcagg
      961 tgttaaagca aaatcagcca acggatattt tacatttgtt tcactatctg tgataactgc
     1021 aaatggtgtt acttctgaac ctgtacctga tgtcgtaggg atacaaatga acgtcgcatt
     1081 ttcaggcatg cctattttat aagtacgttt accgatgtct aggaactttt gtttagcacc
     1141 gaagaatgat gtctcagggt gttcaaagaa catccacatt gcttttgcag catccatcgc
     1201 tgaaccacca ccaagtgcaa tgattgtatc cggttggaaa tcaaccatca tttccagacc
     1261 tttatatact gtattagttg atgggttcgg ttcgacttcg ctaaatattt taatttgagg
     1321 ctgttccgtt ttttgtctta atacattctc aactgttttt gtataaccga attctaccat
     1381 accagggtca cagacaatca tcactttttc aatcttgtcc attgttgtta gactcatgat
     1441 tgcattttct tcaaaataaa tttgagcagg caccttgaaa atttgagtat tattacgtcg
     1501 tttagcaatc gttttaatgt ttaataaatc tgtcgcacta acattatgtg aaattgagtt
     1561 tctaccgtag gaaccacaac ctaatgttaa agacggaatc aattcgttat acatatcacc
     1621 aatacctcca accgctgatg gtgtatttac aagtacacga caagctttca ttcttagtcc
     1681 aaaatctttt tgtaatgttt catcttctgt atggataacg gctgtgtgtc ctaatccacc
     1741 aaaatgtagt gtgtcttcac aaatttgaaa tgcttgtttt gtagattggg cttttactaa
     1801 ggctaatact ggagataatt tttcacgaga taacggatag tctgaaccta caccgctaat
     1861 ttcggctatg ataagttttg tattttcggg gacaggtata cctgctaatt cagctatttc
     1921 aactgcagat ttaccgacaa tatcaggctt aatacctgtt ttttgttcat tcataattgc
     1981 attttctaag cgttgtaatt catctttttt aacaaagtat gcttgatgtg ctttaaattc
     2041 attagtaaca tctttataaa tttctttatc aatgactaca acttgttcag aagcacaaat
     2101 cataccatta tcaaatgttt ttgaaccaat gatatcattt actgcacgtt taatgtgtgc
     2161 tgttttttca atgtaagacg gcacgttacc tggtcccaca cctaatgccg gtttgccagt
     2221 tgaatatgca gacttaacca tgcccgaacc acctgttgct agaactaatg caataccttt
     2281 gtgattcatt aattgttttg ttgcttcgat agaaggcact tcaatccact gaataatatc
     2341 tttaggtgca cctgccttca ttgccgcttc taatacaact tctgctgcac gcttcgacga
     2401 ttcttgtgca cttggatgga atgcaaaaat gattggattt cctgtcttaa ttgcaatcat
     2461 cgctttaaaa atagttgtcg acgtaggatt tgttgttggc gtaacaccac aaataacacc
     2521 aattggttcc gctacatacg ttaatccttt ttctttatct tcaccaataa tccctactgt
     2581 cttattgtct tttattgaat tccatatata ttcagaagcg tataaatttt taatcgcttt
     2641 atcttcgtat atacctcttc cagtttcttc atgtgctaat tttgctagca ccatatgttg
     2701 atcaacagct gctaagctca tttgatgaac aatatgatca atttcttctt gtgacttttt
     2761 agataatgct tctaatgctt ttttcccttt gtcagctaga gcatcaatca taattgccac
     2821 ttcttgttct ttcgatccac gattttcttt ttcaggtata gttaacatat acaaccactc
     2881 ctttatactt tgtgaattat ttcacaaaca ttatagtaca tgtctctcag gatataaaga
     2941 aaattctata caaaaaagtt taatttcgaa tattatttga acaaatatca aattttaaaa
     3001 taaatgtttt catgaaatca ttgttatttc ggtgttttta gaatgatttt ataatcataa
     3061 ttttttcaat gacataattt attcataatt atatatttaa ttctgaatat tctttaatag
     3121 cctaaaaagc ttgttatatc gcacaaaaca caacattaaa aggtccttgt cgatacctat
     3181 caacagatgt tacaaataaa aaccacccgt gtgaacgggt ggtttgttct gcggctataa
     3241 gccttcctta ctggccagcc ctaaaagggc actgacaagt cagccaactg cactactatt
     3301 ccagcaatcc taaaggttta ctcttttttc tttctttttt tatttttctc tccagtgaaa
     3361 ggatctaaat attcttccat tgaaatttca tctgcaacga tatcctcttg taattgatta
     3421 cgaatataat tttcaatcac ttttttattt ctacctactg tatccacata aaatccttta
     3481 caccaaaact ttctatttcc atatctatac tttaagttag catgtctatc aaatatcatt
     3541 aaattacttt tccttttaaa tagccaacaa atgatgatac cccaagtttg ggtggtatac
     3601 taactaacat atggatatga tctttacata cctctgcttc aattatctct acaccttttc
     3661 tttcatataa ttgacgtaat ataatcccta tatctttttt tatttttcca tatatcgctt
     3721 gtcttctgta tttaggtgca aagacaatat gttacttaca attccatttc gtatgtgcta
     3781 aactgtttgt gtcagatgac attaaatagc atctcctcgt gttgattatt ttggttggct
     3841 gaccaatatt tactctaaca tgtagagatg catttttttg acaatggtag aaccttttct
     3901 ggggagtggg acagaaatga tattttcgca aaatttattt cgccgtccca ccccaacttg
     3961 cattgtctgt agaaattggg aatccaattt ctctttgttg gggccccgcc ccaactcgca
     4021 ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccctgacta gaattgaaaa
     4081 aagcttatta caagcgcatt ttcgttcagt caattactgc caatataact tcgtagatca
     4141 tagaagattg atttatttcc cagcctattc tttttataat atttagcaaa ggtaataatg
     4201 atattaataa tgtacaaaaa atataaatca aatcgacatc cttataaaac atcagaacca
     4261 ctaaaaacaa aaaagcacaa aataaaatta aatttaaaat aaacgaccac ttttcaaaaa
     4321 aatctctttt catatttcca cccctaattt taataagcat tattttatat tctcttttaa
     4381 gtttattatt caaaaggaaa acagaaatat ctttcaatat tattataaac atttcaacta
     4441 cttttaaaaa ccaacaaaaa aatacttatt ttaagtagat gagcataagt gaacatagtt
     4501 ctttagttat aataattaat tcaaccaaaa gtcgatttgt ttttgcaatt ggttttcatt
     4561 tcctcttaaa gatattttca ttaaatctgt caaatcaata gacgctatat ttttcaactt
     4621 atctctatat ttatttttag tacgtctttc taaatttccc cattcctctt cttcgtgagt
     4681 taataaatga agcattgctc gttcttgtat attttcaatc atttttaaat tcggttttaa
     4741 aatatgcaaa tcatcaaaac aatctttcca acaatcaacc atatctcgtt ttaattcaat
     4801 ttccacacgc catagaaatg ttgaatcaat ttcaacatct gcattatctt tacgttcttg
     4861 tttttattat aaatccgaat aaacctatca ctattacgca caccaaaata ttttgtttct
     4921 ggttttacat tacgtccata aaatatagtt ttctttaccg acttatctga caatgcataa
     4981 tagtcattta aatcaaattc aaaatcaaaa gccaaatcta atctcgtaaa actaacatcg
     5041 tccaaataac tgatgatatt ttgttttaac caaagcactt catcatgcga aagcttatta
     5101 ggattaaatt caacgcgcat actacgtcta ttccaaagag ttgcttttat tttgtcatat
     5161 tcaatataaa ctttttcttt aagagcttta gctttaaagt ttgtttgtaa aatatcccaa
     5221 agccgaattt caggattagt actcataaaa tgtgaaagtc tctctgcgtt agacatgcta
     5281 agattcccaa caatcgttat agcgtcaaaa gacaattttg gaatagctag tgacatccta
     5341 tgtcgattta accggctatt accggatatt agagtatcca gttttacaaa tggatgaaac
     5401 gaaattcaaa acactaaaaa atatgttcca ctaacagcaa aaaaatacca ttatgttcct
     5461 actaaaaaac taaaaatact ggagaacaaa tgtcaggata taacttagga tactatgtaa
     5521 taaaaattta caataaaaaa acaggaaaac aaatttcaag taaaagaata cccatacaaa
     5581 gaggataaaa taaaaaacct cgaactgaaa tgatgatctt ttcagctcga ggtttaaata
     5641 ttggtgcctt atttatatag attcgttata ttatattctc tattttcatt aacataatcc
     5701 ttaaagagtt ttaaattaat acctgctaga tgattcaaaa atgtttcatc aacttttaaa
     5761 taattcaata atttttgtgg tgtcagtaaa tttctatcaa aatacaactt taataaacta
     5821 ttcattttga caggacgtga catttcaatc acgtcgtcta aagataatac tttctcgctt
     5881 taaacaaaaa caaaaactta cccgattaaa atcaagtaag ttttacatat atccgtcact
     5941 tttcaacttt aaaatcaaca acttgcggga tgcgcatcaa attattattt gataaaaaat
     6001 caataagtaa ttgtgcgcct tcaacttgaa tatcttttac aactggcgcg tcgatataca
     6061 tatcatactg accaccgcct actgcacgat aattatttac acaaattgta tatgtctgct
     6121 ttaaatcaac tgcgtgacct tgaatcatca tattgctcac acgttgtccc tttggtcttc
     6181 caacatgaat ggtataactt acgccaccat atatatcata attaaagtgt tgtggtttgg
     6241 gttcaaggaa gtctgcgctc acactaactt catcattttt cacgtcaaaa tattctgctg
     6301 atcgttcaat ggcttcttta agtttggcac cacttacagc taaaacttta aatgtatttg
     6361 gaaatgggta attgttaata acatctcgca tcgtcacgac ttgcttgaaa ccactagcag
     6421 aatcaaacaa agctgtacag gcaacatctg cgtcactttt ttctaataaa gcgtaattca
     6481 taaaatttgt aaaaggatgc ggtgccacac gtgcctcaaa tgcatgatta atcgtcatat
     6541 catatggcaa tgtagtaatt tcgtaatcta accagtcctc taactgcttt cgtaaatgtt
     6601 ggtcatcttc atcaatagta aatgtggaat catctataac aggaagtaat tcacatgatt
     6661 caacggatag attttcatat tcatcagtac tcaagactac tctgcctaca gttgtacctc
     6721 tcgtaccagg ttgaatcaca gccgtttgct taaacctttc agcaatttgt cgatgttggt
     6781 gacccgtaat aaagatatct atatctttag aaaacgcttc taacatggca tatccttcat
     6841 tttcacccgt taatacttcg gtcggcgtac cactttctaa atccttttca aatccaccat
     6901 ggtaacaaac cacaatgata tctgcatgtc gcttcatttc aggtaagtat tgttgaagta
     6961 tttcaaaagc actatgaaac gtaagtgact gaatatgctc tggttgttcc caatggggaa
     7021 taaattgtgt cgttaaacct atcacaccaa cagtttgatc tccaacctga aaatacttca
     7081 caccgttatc agtcaatgta ctatcatttt catatatatt agcgcacaaa actggataat
     7141 tgagtctgcg taaagtgtct tttaagtatg gtaatccata attaaattca tgattaccaa
     7201 gcgtaccaaa gtcgaatgcc attcgattat aaaaatcaac taaaggctgg ctactgccgc
     7261 tatgcgcgat taagtaatta caaaatggtg acccttgcaa aaaatcacca ttatctattt
     7321 taaaactttg gtcatactgc cttctgtctt gttctataac atgattcgct agtaacaatc
     7381 ccataggttg atattgattt ctactcgtaa aatctgttgg gaaaatataa ccatgtacgt
     7441 cactcacgac ataaaatgct atgtttgaca tcctcactca ctccttcaat cacaaacatc
     7501 tttcttattt ctattatata tttatttgaa gtctgttgta atcaaggttt tgtcaccgag
     7561 ttttaaacga atctttgaac cttccatact ttcaagtact ttagcattga ccttaattgt
     7621 gacatttccg ttttcatctg ctttaactgt tggcaaagta ctgtaacctg gtgggttata
     7681 atcgttatct ttacttgaaa attgtccgat ttgacgtccg ccttctttgt aaataccaac
     7741 tctaagattt tcaagtgttt gatttgcttc aaatcctttt aaatgtattg tcatttcaaa
     7801 tggctcattt acagaaacat tttgcgggat atcaaatgtt actttttcgt tctgatttgg
     7861 tggtgtatga tcatctggtg tgtttggctg aggatctgcg cctttttcgc tgccataact
     7921 acctgcttta aatgttgttg gatcatacca tttataacca ctcggcggtt gtgaccatgg
     7981 ctctttttca ggctcagttg aacgctctgg tcgttcaaaa tcaagcaact tagtctttgt
     8041 atctaatgtt aggctactcg ccttaagtga tttcccatca ttatctttag acatccaagc
     8101 cgttatatta tttaatagct taccgttgtc ttgttcttta aaaccatcat atgttttctt
     8161 cttttctcca ttatcttctc ttacatattt gggcgaacta tcttccacaa gtgatgaatc
     8221 accgataaat gctgctttac cttttccaac tttagaaatt gctacatagg ggccttctgc
     8281 tttaccgccc ccattataaa taccttgatc tacagcatgt gaccatttac ttttcgctgg
     8341 caattgttct ggtgtataca caataccttt tgctttctct ggattagtaa ttgctaatgt
     8401 cgatccggca tgcatagaga cagatttcac accttcagta ataccgaaac tttcttttga
     8461 agaaacaata ttgctcgtat ttaaatcacc tagtgcatta tatcgaaaac gtacgccaaa
     8521 gtttgtagat aaccaatctg aacttttcac accttgcatt gcagtagaac ttttttcttc
     8581 tgcattcata cctttcgaca tatcttcata tgctccacgt cgataaccat tcattgcctc
     8641 cgatgaatca atacgattta aatttcggtc agcattgtaa tgatctgaaa taaagacaac
     8701 attgccacct tgtttcacat atttaacaat tgctgcctgt tctgattctt tgaaaggaat
     8761 gttagcctca ggaattacaa atattttgga acttttcaaa cttgcttctg ttatgttcga
     8821 atgaccatca atagctttaa cgtcataacc ttgtttttgt attgaatccg cataatctga
     8881 aaatgcacca tcactaaccc aatctgcagc accagctgtt tgaccatgag aacgatcgaa
     8941 taataccgtt cgctgttgct ttgtaggttg cgattcatgc gttatagcta aagattgcgg
     9001 taaagcactt aatgataccg ttgcaacaat tgcagagaca gttaatgact tatatatttt
     9061 tttcattttg tgaggctcct tttaaaataa atttgttctt gaattatagg ataaaaattc
     9121 gttgcatatg agcaatttaa cgaaaaattt acaaaatctt atcaaactct taaagaaagt
     9181 tattaaaatt catttttata aaatactttt taacatttaa atgtggtacg ctataagtgt
     9241 aatttcattg catacatatt acacgattaa gaatgtgaag gggacagtta tcaaatgaaa
     9301 aattttaagt gtttatttgt attaatgtta gcagtcattg tttttgcagc agcatgtgga
     9361 aactcaagtt ctttagataa tcaaaagaac gctagtaatg attcggattc taaatcagga
     9421 ggatacaaac ctaaagaatt aaccgttcaa tttgtacctt cgcaaaatgc tggaacatta
     9481 gaagctaaag caaaaccatt agaaaaatta ctatctaaag aattagggat tccagttaaa
     9541 gtgtctgtat caactaacta caatacaatt gttgaagcta tgaagtctaa aaaagttgat
     9601 gttggtttct taccaccaac ggcatacaca ttagcacatg atcaaaaagc agctgattta
     9661 ttattacaag cacaacgttt cggtgtaaaa gaagatggtt cagcaagtaa agaacttgta
     9721 gatagttata aatcagaaat tcttgttaaa aaagactcaa aaattaaaag cttgaaagat
     9781 ttaaaaggta agaaaattgc cttacaagat gtaacatcaa ctgctggata tacattccca
     9841 cttgcgatgt taaaaaacga agcaggtatt aatgcaacta aagatatgaa aattgtgaat
     9901 gttaaaggtc atgaccaagc agttatctca ttattaaatg gagatgtaga tgctgcggct
     9961 gtatttaacg atgcacgtaa tactgtgaaa aaagaccaac caaatgtatt taaagacaca
    10021 cgaattttaa aattaacaca agctattccg aatgacacaa tttctgtaag accagatatg
    10081 gataaagatt ttcaagaaaa attgaaaaaa gcttttatag acattgctaa atcaaaagaa
    10141 ggtcacaaaa ttattagcga agtttattca catgaaggat acacagaaac gaaagattca
    10201 aatttcgaca ttgtaagaga gtacgaaaaa ttagttaaag atatgaaata atcattattt
    10261 aacaaatgaa tcattagcga atttggtatt aaaagctttc gttcaataga tatattctag
    10321 attaatattg aaaagctagg cgctaaactg aaacagatat agaaaggtgt cgctgtacat
    10381 ttgaaaccat ttgtacacag aaacccaatg tctatgatat ttcagtttac cttggctttt
    10441 ctttattaaa gaaaggtgtc aaacatgagt caaatcgaat ttaaaaacgt cagtaaagtc
    10501 tatcctaacg gtcatgtagg cttgaaaaat attaacttaa atattgaaaa aggtgaattt
    10561 gcagttattg tcggactatc tggtgctggg aaatccacgt tattaagatc tgtaaatcgt
    10621 ttgcatgata tcacgtcagg tgaaattttc atccaaggta aatcaatcac taaagcccat
    10681 ggtaaagcat tattagaaat gcgccgaaat ataggtatga ttttccaaca ttttaattta
    10741 gttaaacggt caagtgtatt acgaaatgta ctaagtggac gtgtaggtta tcaccctact
    10801 tggaaaatgg tattaggttt attcccaaaa gaagacaaaa ttaaggcaat ggatgcacta
    10861 gaacgcgtca atatcttaga taaatataat caacgctctg atgaattatc aggtggccaa
    10921 caacaacgta tatctattgc acgtgcgcta tgccaagaat ctgaaattat tcttgcagat
    10981 gaaccagttg cttcattaga cccattaact acgaaacagg ttatggatga tttaagaaaa
    11041 atcaaccaag aattaggcat cacaatttta attaatttac attttgttga cttggcaaaa
    11101 gaatatggca cacgcatcat tggtttacgt gatggtgaag ttgtctatga tggtcctgca
    11161 tctgaagcaa cagatgacgt atttagtgaa atatatggac gtacaattaa agaagatgaa
    11221 aagctaggag tgaactaaca tgcctttaga aatacctaca aagtatgact cccttttaaa
    11281 gaaaaaggtt tctttaaaaa cgagttttac cttcatgtta atcattgtgc ttatcatttg
    11341 gagcttcatt tatacgggat ttaattttgg agatttaatg ataggaatac ctcaaatagg
    11401 tgatctattc aaacaaatga ttccacctga tttcgagtat ttacaacaaa ttacaacgcc
    11461 aatgttagat accattcgaa tggctatcgt aagtacagta ttaggtagca tcgtttcaat
    11521 accaattgcg ttattatgtg ctagcaatat cgttcatcaa aagtggattt caataccctc
    11581 gcgctttatt ttaaatatag ttcgtactat tccagatttg ttattagcag caatctttgt
    11641 ggctgtattt ggaatcggtc aaattccagg gatattagca ctgtttattt taactatctg
    11701 tattattgga aaattattat atgaatcatt ggaaacgata gatccaggtc caatggaagc
    11761 aatgacggct gttggcgcta ataaaataaa atggattgtt ttcggtgttg taccacaagc
    11821 catatcgtca tttatgtcat acgtattata tgcatttgaa gtaaatatac gtgcttcagc
    11881 tgtgcttgga ttagtcggcg ctggcggtat tggattgttt tatgatcaaa cacttggttt
    11941 atttcaatat ccaaaaacag caacgattat tttatttact ttagttatcg tcgtcgtcat
    12001 tgattacatc agtacgaaag tgagggcaca tctcgcatga cacaggaaat agcaaaatat
    12061 aatgttcaca caaaagcaca caaacgaaaa ttgattaaaa gatggcttat tgcaattgtc
    12121 gtcttagcta ttatcatctg ggcatttgca ggtgtaccaa gtttagaact taaaagtaaa
    12181 tcattagaaa tcttaaaatc catattcagc ggattattcc atcctgatat cagctatatc
    12241 tatataccag atggcgaaga cttattacgt ggtttacttg aaacctttgc gatagccgtt
    12301 gtaggtactt tcatcgccgc aattatctgt attccattag catttctagg tgcaaataat
    12361 atggtaaagc tacgcccagt ttcaggtgtt agcaaattta ttttaagtgt tatacgtgtc
    12421 ttcccagaaa ttgtaatggc acttatattt atcaaagctg ttggcccagg ttcattttca
    12481 ggtgtattag ctttaggtat ccattccgta ggtatgcttg ggaaactttt agctgaagat
    12541 attgaaggtc tagatttcag tgctgtagaa tcattaaagg ccagtggtgc gaataagatt
    12601 aaaacactcg tatttgcagt cataccacaa attatgcctg cctttctatc actcatactt
    12661 tatcgctttg aactaaactt acgttcagct tctatactgg ggctaattgg ggctggtggt
    12721 atcgggacac cactcatatt tgccattcaa acacgttctt gggaccgtgt aggtattata
    12781 ttaatcggtt tagtactaat ggtcgcaatt gtcgatttaa tttccggttc aatccgaaaa
    12841 cgtattgttt aacattaaat caggatactc ctaaataaga agtcctaccg tcttacgttt
    12901 ctctattata ataaaaacag cagtgaagaa aactattgtt atagttaact tcactgctgt
    12961 ttttataata tctaaattta ttctatttca attcctttaa ataactttta ccgaactctg
    13021 gtaatgttac gttgaaatta tctgctatag ttgcaccgat agaactgaat gtagtatcac
    13081 tttctagtgc atgaccacct ttaaatttcg gactgtacat aattactggg atatattctc
    13141 tcgtatggtc cgtacctggc gctgtcgggt cattaccatg gtctgctgta ataattacta
    13201 aatcgtcttc ttttaagttg ctaaacagtt ctggcaagcg atcatcgaaa tctttaattg
    13261 cttgtgcata acctggttta tcacgacgat gaccgtataa tgcatcaaag tctactaagt
    13321 ttaagaagct aatacctgtg aaatctttct taacaatttt catcaattga tccataccgt
    13381 ccatgttact cttcgtacga accgcttctg ttacaccttc accatcataa atgtcattaa
    13441 ttttaccgat ggcaataaca tcataaccac cgtctttcaa atgatctaag acagttttac
    13501 caaaaggttt taacgcatag tcatgtcgat tagatgtacg tgtaaagttt cctggttcac
    13561 caacatatgg acgtgcgata atacgaccaa ttaaatattt agggtctttt gtcaactcac
    13621 gaaccttttc acaaatatca tataactctt ctaatgggat aatgtcttca tgtgcagcaa
    13681 tttgcaatac tgggtctgca cttgtataaa caattaagtc accagttttc atttggtgct
    13741 cgccccactc atcgataatt tgcgtacccg atgccggttt gttagcaaca actttacgac
    13801 ctgtcatttc ttcaatttgt tgaattaact cttcagggaa tccattaggg tatactttaa
    13861 aaggttgcat aatatttaat cccataattt cccagtgacc agtcattgta tctttaccaa
    13921 ctgaagcttc actcaattta gtatagtatg cttctggttg ttcaactgca tttactactg
    13981 gtaatttatc gatgttccct agacctaact tttcaaggtt tggtaaagtt tgatcgaaac
    14041 cttctaaggt atgtcttaaa gtatgtgaac cttcatcttt aaaatcagct gcgtctggcg
    14101 cttcaccaat acctactgaa tccattacga ttaaatgtac acgattaaat ggtcttgtca
    14161 tagctatcac tcccaaaatt tatatatatt agtaatctga atctgcttct aaaccttgca
    14221 taatttgaac acctgcgctc gcaccaatac gtgtcgcacc tgcttcaacc attttattga
    14281 aatcttctaa attacgtacg ccacctgatg cttttacttc tacatcagca cctactgtat
    14341 ctttcattaa tttaacgtct tctgcagtcg caccgccacc tgcaaaacct gttgaagttt
    14401 taacgaagtc cgcaccagcc gtttttgtta attcactcgc ttttacaatt tcgtcatggt
    14461 ccaacaatac cgtctcaata atcactttta ctgtgtgacc tttcgcagct ttaaccactg
    14521 cttcaatgtc ttgttgtaca tcatcaaaac gtccatcttt taatgcgccg atgttgatga
    14581 ccatgtcaat ttcatctgca ccattttgaa ttgcatcttc tgtttcaaat gctttcgttg
    14641 cagttgtcga cgcacctaat gggaatccta ttaccgtaca aacgagcacc tctgaatcag
    14701 ctagtcgctc tgctgcatat ttaacatgtg ttggattcac acatacagat ttaaaattgt
    14761 atgctttcgc ttcatcgatg atttgatcga tttgcgtacg tgttgactca ggcttcaata
    14821 aagtgtgatc tatatatttc tcaaatttca tacttactac tcctcgtgtt atataatctc
    14881 tttatttaat tttactataa atacgaatat atctcgcgaa tttatatcta tttatttttc
    14941 agattgtgtc caacgattta agctgacgaa taaaactaat ccgacaatac tcattaaacc
    15001 taaaataatt aaaataatac cgaaatgtga acttaatgca tcattgcctg ggaaatttaa
    15061 tgctttaaaa tcgattagag ccgcagcaat cgcaatacct acagataccg ccacattaat
    15121 aattaaatta taaaaaccaa tagccacacc tgtcatatta agatctattg ttttaatggc
    15181 ttcgttaagt aaaggtgcat acattaaagc aaagctacct gcaaagaata tcatagaaat
    15241 gacgaagatt gaaatgtgat tacctactgc aaatgcaggt aaaatcaagc tcagtgctat
    15301 taaaataatt gctgtgataa tcgcttgttt tgaattcaga tattcgccga ttttaccact
    15361 tagtgcacca acaatgactg ctactatata acccggtact aataacagtg atgttgtgtc
    15421 tagttgcaga tgataaattt gctccattat gaatgggaac gtaaaaatat aacccaattg
    15481 gatagcatac attacaaata ctataaataa aaatgaagca taacgtttat tttggaaaaa
    15541 tgatttattt actaatggac gttgcgcatt tttaatatat agcgcaaaaa cgataatcgc
    15601 aattaaggca ccaatcatat ataaccaatt aaagttcgta ataaacagca tgactgttgt
    15661 agcaatagtt gccactagaa ttaagccaac aaaatctaaa tgagctttat tcgtattatt
    15721 ttcttttggt aataatttaa ataggaatgg caacgtaaat actacgatta atgcaattaa
    15781 aaacatattt gtccagtgta aatacgtaga aataaatcca cctgataatg taccgataac
    15841 taatgacaag gaataactgc tcgtacttaa gccaaggtaa gtcttctggt cctctttaga
    15901 aagatacttt gcaacatata tcacatataa tgtctctgca gcagctaaac cggcagtttg
    15961 aataatacgt ccaactaaaa gtaatgggaa ttgatgttgg aaaatgtaac caataattga
    16021 tccgataatg attaatatca cgccataaat aaatagtgtg cgtatactaa tggcatcaga
    16081 taatgaagcg tatactacag cgccaatacc aattactaaa ccggctaatg tcgcttgcca
    16141 acttactgtc gttacagata tgtttaagtc atcagcaatg gctacagaaa tcaatttaaa
    16201 tgagttgtct ataaccaaac tgaatacaaa taagaataat aaaataggta ctgctttctt
    16261 tattttagaa tattcaacat tcataatcat ctacaccaat gacagtgcaa tttcaatcat
    16321 atctgtaaat gcacgttccc tttcctcagg tgttgttgac gtttcatgaa ttaaatgatc
    16381 gctcactgtg aacacaccta atgcttcgac accagcgtaa attgcattca tgtataatgc
    16441 agctgattcc atttctacac ctaaaatacc catacgcatc caacgttcac tcgctgttgt
    16501 atccgcgtta tagaaaatat cacttgataa cacattacct acatgatgac gtacaccttt
    16561 gtcacgtgct gtttcaactg ctttttctaa taattgataa gaagcaattg gcgcaaaatg
    16621 acctggtaat tgatattgtt gaacgtaatt tgaatcagta gaggcacctt gtgcaataat
    16681 cacatcatat aaatcaatgt tttcttgcat cgcgccacaa gagccaacgc gaattaattt
    16741 tttacaacca aatgtatgaa ttaattcata agagtaaatg ccaatagatg gcatacccat
    16801 acctgaaccc atgacagaaa cttttttacc tttatatgtt ccggtaaaac caaacatgtt
    16861 tcgcactgta ttgaactgtt ccacatcatc caaataagtt tctgcaatga acttagctct
    16921 taacggatct cctggcaata gaaccgtttc tgcaatttcg acgtcattca ttggtttaat
    16981 gtgtggtgtt gatttcataa aatattcctc ccaatatttg tatagtgatt tgtctatttg
    17041 tctaaccatg tatataataa tattttttaa aagcgctttc aagcattaca ttttgacaat
    17101 attaagaaca ttttacagtt acagctacta accttgtata aaactaattt tcaaagccta
    17161 tctatattta atttttataa tttttccaat ttgagtaaaa attattgtat gatgagagtg
    17221 attaagtaac tttatggagg ttgtctacgt gacgcaaaca caaccaaaat ttttaacgat
    17281 ttataatacg ttatataaag aaattcaaat aggaaaatat ccgagtggcc atgcattacc
    17341 tactgaaaag gaactttgtg cgcgctttga tgtcagtcgt atgactttgc gccaagctat
    17401 aaaattgtta gccgaagatg gcatcgtaga gagtacaaga gggaagggac actttgttat
    17461 tccacaaaca aatgcccaac acgcttcaag tatggatcaa tttaagcatc cacttgatca
    17521 aatattgcta gctcgcgtat ctatgtcatc aattaattat cgtgttgatt tagaaagtga
    17581 atatacaaat catttgtttg caaaccatcc ttctgccgtc attgcaatgg aacgctatta
    17641 tcaaaaaaag gacaatcatt ctaagcaagc tgatgcattt tgctttacct ttataccgtt
    17701 aaatgtcatt gacacattta aagtgaatac acaaaacgaa gacgacatga agacattcgt
    17761 tgagcaaacg atttatagca atgcatatca atcagattta aaaatgtcta ttaccaaagc
    17821 gccccacttc aaaaatcata gtcacgtgtt tgatggtgat acacattgtt ggctgattat
    17881 tgaaacactg tacgcacaaa caccttatcc aattatgatt aataagtggt atattccaca
    17941 ggaaatatct gaattaacac tgacacgtat aagacaatcg gattattaaa aattattaaa
    18001 atataagaaa ttttttgatg actatttagg gagttggtaa gaaatggaaa agcttgttac
    18061 aagcgcaatt tcattcagtc aactactgcc aatataacat tagcgagcct aggacattga
    18121 tttatgtcct aggctcgctc agataatcac acgatttttc tattattaat cgtattatgt
    18181 ttatttattt gttgaacgtc gtctaaatgc taagaataac ccagatacta atagtagtgc
    18241 ggcaatactt gcagtaagaa tacttgatgt catactttca ccagcagctg gtaaaacttt
    18301 tttgttactt tggttcattt gatgatttga tgaatgcata tttgcttttg gactcatcat
    18361 ttgatggttt gatgattgca tatgaccttg ttgcatgtgt ggctgttggt tcatgtgtgt
    18421 gttctgattc atatggcctt gttgactcat atgacctgtt ttattttgca ccatttcact
    18481 accaccatga ttaacaccat ttgcatctac attcgtttga taaaaattgc catcttttgt
    18541 taaaggtaac gcatggccat tcatatctct aacagcatgt ggtttaaata cccctacatt
    18601 agttgtaatt atcacattgt atttatcagc aatattttgc gtcgcatcat acaatttcat
    18661 attatagtct tgcatattac tgccagcgct taattggggt aaagctttct ctaattcagt
    18721 atattgtgct tgcgtcattc cttgtggtac tactccgcca taaggacgat gattttgtac
    18781 attttggctt tgtttattcg acacattcat tgaagttgtg tcggcatgcg cattttgaaa
    18841 aggcatactt gagaatacta aagtgcttgt tacaattaaa gaacctactg ttgctaattt
    18901 tttcattgtc ttgtcccctt atattacaat ttgattacat ttacattatc atacgattac
    18961 aaaagaaatg caacaaaatt tttgaatcat tacatttttt tataaaaatt tcactttaga
    19021 ttcacaataa ttacttattt tgtcaattta tttaatgtca atatgttgat taattaatag
    19081 tgttgtctaa tgtatataat atttaggtca tcgttatagt caacaataat aaggtatttc
    19141 gagttgaaat ttatcttatt atttttccac ttttacgtgc tatcccatta cacaaaaaca
    19201 aataagtaaa gatattaagt acaaaaaagt ggaacacctt gtagatgctc cacctcaatt
    19261 atattaagtt atattatttt gctgcttggt ataattcatc aacttttttc cagttaacaa
    19321 tgttccaaaa tgcagtcata tagtctggac gtttattttg atatttcaga tagtaggcat
    19381 gctcccaaac atcaaatagt aagattggtg ttttgccttc tgttaatgga ttatcttggt
    19441 ttggcgttgt cacaatttct aatttgccat cattaacaac taaccaagtc caacctgatc
    19501 caaataatgt tgttgcttta tttgcaaatt catttttaaa ttcatctaaa gtgccccact
    19561 gcgctttgat gtcatctatt acgccacctt tttcttcaga attaggtgat agtatttccc
    19621 agaataatga atggttaaaa tgaccaccgc cattattacg gactgacatc ctcatcgctt
    19681 ccggtacctt gtctaagtta gcaatcatat ccgctagtga ttgatgctct aactctgttc
    19741 cttcaactgt tgcgtttaat ttcgtcacgt acgtattgtg atgtttgtcg tgatgaaact
    19801 ccattgttct ttgatctata tatggttcca atgcatcata tgcatatggt aaatttggta
    19861 atttaaatgc cataagtata ttcctccttt tatgaatata cttttataat aattaatttc
    19921 gggtgtgttt tgcaataatt attatttatt tgttatatcc aattatataa aatttaaata
    19981 ttcgaaattg atataatat
//
LOCUS       DANJFZ010000043        19895 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.043, whole genome shotgun sequence.
ACCESSION   DANJFZ010000043 DANJFZ010000000
VERSION     DANJFZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19895
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.043"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(115..1140)
                     /locus_tag="PWN45_001346"
     CDS             complement(115..1140)
                     /locus_tag="PWN45_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDL0623116.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            1410..1607
                     /locus_tag="PWN45_001347"
     CDS             1410..1607
                     /locus_tag="PWN45_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623117.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            1665..3146
                     /gene="cls"
                     /locus_tag="PWN45_001348"
     CDS             1665..3146
                     /gene="cls"
                     /locus_tag="PWN45_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0623118.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            3321..4229
                     /locus_tag="PWN45_001349"
     CDS             3321..4229
                     /locus_tag="PWN45_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623119.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            4198..4929
                     /locus_tag="PWN45_001350"
     CDS             4198..4929
                     /locus_tag="PWN45_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623120.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAIILLYALGSIIAVYCISHFKRAE"
     gene            4933..6024
                     /locus_tag="PWN45_001351"
     CDS             4933..6024
                     /locus_tag="PWN45_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0623121.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            6021..6623
                     /locus_tag="PWN45_001352"
     CDS             6021..6623
                     /locus_tag="PWN45_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0623122.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(6736..6924)
                     /locus_tag="PWN45_001353"
     CDS             complement(6736..6924)
                     /locus_tag="PWN45_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDL0623123.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            7063..7596
                     /locus_tag="PWN45_001354"
     CDS             7063..7596
                     /locus_tag="PWN45_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL0623124.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(7739..8593)
                     /locus_tag="PWN45_001355"
     CDS             complement(7739..8593)
                     /locus_tag="PWN45_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623125.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            8924..9499
                     /locus_tag="PWN45_001356"
     CDS             8924..9499
                     /locus_tag="PWN45_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623126.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(9553..10935)
                     /locus_tag="PWN45_001357"
     CDS             complement(9553..10935)
                     /locus_tag="PWN45_001357"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL0623127.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            11126..12406
                     /locus_tag="PWN45_001358"
     CDS             11126..12406
                     /locus_tag="PWN45_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDL0623128.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            12412..13473
                     /locus_tag="PWN45_001359"
     CDS             12412..13473
                     /locus_tag="PWN45_001359"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL0623129.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            13475..14389
                     /locus_tag="PWN45_001360"
     CDS             13475..14389
                     /locus_tag="PWN45_001360"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL0623130.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            14447..15250
                     /locus_tag="PWN45_001361"
     CDS             14447..15250
                     /locus_tag="PWN45_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0623131.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(15543..15857)
                     /locus_tag="PWN45_001362"
     CDS             complement(15543..15857)
                     /locus_tag="PWN45_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623132.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(15832..15936)
                     /locus_tag="PWN45_001363"
     CDS             complement(15832..15936)
                     /locus_tag="PWN45_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623133.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(16075..17529)
                     /locus_tag="PWN45_001364"
     CDS             complement(16075..17529)
                     /locus_tag="PWN45_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0623134.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            17733..19250
                     /locus_tag="PWN45_001365"
     CDS             17733..19250
                     /locus_tag="PWN45_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL0623135.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            19341..19490
                     /gene="rpmG"
                     /locus_tag="PWN45_001366"
     CDS             19341..19490
                     /gene="rpmG"
                     /locus_tag="PWN45_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0623136.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
BASE COUNT         7145 a         2767 c         3363 g         6620 t
ORIGIN      
        1 attttatatc atcaacggct acaaatgagc agtttcatat ataaataaac acatctactc
       61 aactgatctt aattaattaa taaatgattt accagatgac atataacagc attattagat
      121 taattctaat agtttattta aattttcttc ggttgtcgcc caactggttg cgaatctaac
      181 aacacgatgt tgatcatcgt atttttccca aacagcaaat ttaacttttt gttctaactc
      241 tgctattttc tcgttactta aaataaaaaa ttgttgattg gttggagaat caaagtaaag
      301 acgatagcct ttatttttaa acccgtcttt catcttattt gccatttcga tagcatgtct
      361 gcttatatta aaatataaat tgtccgtaaa taattctaaa aattgtatgc ctgttaaccg
      421 tccttttgct aaaagggcac cgtgatgctt gattcgagtg gtaaattgtt tcggttcatt
      481 atttttcgta aaaacaatgg cttccccgca taatgcacct atcttcgtac cacctatata
      541 aaatacatca caatatttag cgatgtcttt aatagtcata tctgattggt cactcatcaa
      601 tccataccct aatcgtgcac catccataaa taatggaagc tgatattgct tacatacttt
      661 gcataactct tccaattctg atttagagta taatgtgcca tattctgtag gatgagaaat
      721 atataccatt cctgggaata ccatatggtc ctttttaaaa tcacttttaa atgtctccat
      781 gtaagtttca acatctgaag cactaacttt tccttcctta gagggtatag taattacttt
      841 atgtccacta tattcaattg caccgccctc atgcacagca acatgaccag tgtctgctga
      901 aatgacccct tcgtaacttt ctaacattga attaataaca acctgattgg tttgtgttcc
      961 acctactaaa aaacgaattg tagcatttgg acagtcaatt gtatctttaa tcttttcaat
     1021 tgcctgagct gtgaattgat caaagccata tcccgaagct tgtacaagat ttgtatctac
     1081 taatcgtttt aatacttttt catgagcacc ttctaaataa tcattttcga atgaaatcac
     1141 tacatttccc cctaaaacta atatcaacat tttaataaga taaaccaatt tcaaaactag
     1201 ttcgatattt aaaatgtatt atgaatggtt aaagtttgta tcgcattatc gcgaagttga
     1261 ataaatatat tatacatcaa acaatacatt atgaaaatta attaattttt tatcacagcg
     1321 acttttcatg ctggaacaca tgtattaatg attgaatttt actgttaaaa gttctatgat
     1381 ttaggaaata gagaaaagag gggatttaaa tggaacaaat taaacttaaa acttttacag
     1441 ctgagacttt agagttatta gaaaaaaaca ttaatgcttt tttaagttct gaagaagcta
     1501 caaatttaaa attagtaaat attactatta aagaaataga agaaagaaca ttcccaaata
     1561 atgaagaaga attcaatgca attttaactt tatctgtgaa taaataatat gacgtgaaag
     1621 gtgcaaaatg tttttttgtg cctttcttta taggagaaaa gactatgcga tttacatttt
     1681 caaacgattt aggaacgtta tttactatta ttttagccat tggattcatc attaatttag
     1741 tattggcttt tattattatc tttttagaaa gaaataggcg tacagcgagt tcaacttggg
     1801 catggctatt tgtacttttt gtcttaccat tgattggttt tattctttac ttgttttttg
     1861 gtagaaccgt ttcggcacgc aaattgaata aaaacaatgg taacgtgtta acggatttcg
     1921 atggactttt aaaacaacaa atagaaagct ttgataaagg taattatggt actgataaca
     1981 aacaagttca aaaacatcat gatttagtac gtatgctttt gatggatcaa gatggttttt
     2041 taactgaaaa taataaagtt gatcatttca ttgatggaaa tgatttatat gatcaagttt
     2101 taaaagatat taaaaatgca aaagaatata tccatttaga gtactatact ttcgctttag
     2161 atggtttagg taaaagaatt ttacatgctt tagaagaaaa attgaaacaa ggtctagaag
     2221 taaaaatatt atatgatgat gttggatcta aaaatgttaa gatggcaaat tttgatcatt
     2281 ttaaatcgtt aggtggagaa gttgaagcat tttttgcttc aaaattaccg ttattgaatt
     2341 tcagaatgaa taatagaaat catagaaaaa tcatcgtaat cgatggtcaa ctaggttatg
     2401 tcggaggatt taacattggt gatgaatatc taggattagg aaaattagga tattggagag
     2461 atacgcattt acgtatacaa ggggatgcgg ttgatgcact gcagttgcga tttattttag
     2521 actggaattc gcaagcgcac cgtccacaat ttgaatatga tgttaagtat ttccctaaaa
     2581 agaacggacc attgggcaat tcaccaattc aaatagctgc aagtggcccg gctagtgact
     2641 ggcatcaaat tgaatacggt tatacaaaaa tgattatgag tgcaaagaaa tctgtatatt
     2701 tacaatcacc atatttcatt ccggataatt catatataaa tgccattaaa attgctgcta
     2761 aatcaggtgt agatgtacat ttaatgattc catgtaagcc agatcatcca ttagtatatt
     2821 gggcgacatt ttcaaatgcc tctgacttat tatcaagtgg tgttaaaatt tatacgtatg
     2881 aaaatggatt tatacattct aaaatgtgct taattgatga tgaaatcgta tcagtgggca
     2941 cagcaaatat ggactttaga agttttgaat taaattttga agtaaatgcc tttgtatatg
     3001 atgaaaatct tgctaaagat ttaagggtgg cttatgaaca tgatattaca aaatcaaaac
     3061 aactaaccaa agaatcatat gccaatagac cgctgtctgt taaattcaaa gaatcgttag
     3121 caaaattagt ttcgccaatt ttataattta tttgtaagga gtctcgatta tagaggctct
     3181 ttttattttg cccaaaatta aaataacgat aaatatatta tgtaaatcta ataatgacat
     3241 ttgtcatttc aaatctatga caaagtgttc tattttaaat ctaataaatt tactaatatt
     3301 aaattaagaa aaaaatagag gtgattaaat tgattcaaat atctaatatc aacaagtcat
     3361 ttaataaaag atgtgttcta aaaaatattt cgttcgatat tgaacaaggt aaatgtatcg
     3421 ctttaattgg aaaaaatggt gctggaaagt caacgttaat tgatatatta attggtaatg
     3481 ttaatgctaa ttctggtgag atatttgata aagacaagtt attacaaagt gaaaatcgca
     3541 gtataatgtt ccaaaaaacg atgtttccag atcaattaaa agttattgag attatcaact
     3601 tatatcaatc attttacgaa aatccattac cattggaaga aataatagaa ctgacgaaat
     3661 ttgattctag tcaactgaac caatttgtaa ataaactttc tggtggtcaa caacgattac
     3721 tcgattttgt attatcttta atcggacaac cacaattgat cttattagat gaaccaacat
     3781 cgactatgga tatagaaatt agagaatatt tttggtcaat tattgaaaat ttaaaagaag
     3841 ataatcgaac gatactctat acatcgcact atattgaaga agtcgaacgt atgtcagaca
     3901 aaattattct cattgaaaat ggagaaataa tacttaatga ttcaacgtca catattagaa
     3961 ccaatcagca atctcagatt acgttatccg atgaatatat aagaaagtta aaactagata
     4021 aagatgattt agttattcaa aaaaatcata atggcactat caaaattatt acttcaaatg
     4081 taaatgatac gattttatat cttcaacaac ttcatattaa tttggatgat attgaaatac
     4141 aaaaagtctc aattgttgat tcatacttca acaataaaaa gcaaagggga tctaattatg
     4201 atactaagtt acttgaaaat cgaatttaaa gttataatgc gtaaaaaaac aacattaata
     4261 ttatctattt tatttcctgt tatattctat atattattta cttcgatatt ggaattgccg
     4321 gaagatgtta aacctaaatt ttataaagag tatatgtata gtatgacggt ttatagtttg
     4381 ttaagtttta gtttactaac ttttccatta gatattatta atgaaaaaca aaatgaatgg
     4441 cgccaaagat taatggtaac accatttact tttactagtt attatatttc aaaagtagtg
     4501 aaaactatgc tgcaatttgc aatagcgata ttagttattt ttatggttgg acatttttat
     4561 aaaggtgttg caatgagtgc agttcaatgg ttagagtcag gaatattttt atggttaggt
     4621 gcgtctctat taataacttt tggcatatta ttttctttgt taaatgatat tcaaaaaaca
     4681 agtgctttag ctaatatcgt aacaattggt ttagcagtat taggtggatt gtggtttccg
     4741 ataaacacat ttccaaattg gcttcaacat gttgctcatg ttttaccgag ctatcatttg
     4801 cgtaaactag gtgtagatat tgcttcaaat catcatatca atttaatatc atttgctata
     4861 atactcttgt atgctttagg gagtataata gcagtatatt gtattagtca ttttaaaagg
     4921 gcggaataaa atatgaaatt tttaaaagat acttcaattg ctgaaatatc gtctatagtt
     4981 tatctgattt ttcctattgc cggtatattt tttaatgaag tatatggtcc caaatggttg
     5041 tatattatat cagtcattgt cttttcgttg tcgtatctta tattagttat agtaaataat
     5101 agacttaata cattaatgtt ttacattttg ttgattattc attattttat tatttgttat
     5161 tttgttttca gtgtacatcc aatgctaagt ttgtttttct tttatagtgc ttttgccgtt
     5221 ccatttactt ttaaaaataa tgttaaaaaa acggcaacta atcttttcat actaacaatg
     5281 attatatgta caataataac gtacttattg tataacaact attttgttgc aatgatggtt
     5341 tattatgtcg ttatatcgtt aataatgcta gataatttta aaaaaatgaa aaaccgtgaa
     5401 tatcaaaaag aaatagcaga aaaaaataga catattaata cattaattgc tgaacaagag
     5461 cgacatagaa ttggtcaaga cttacatgat acgttagggc atgtgtttgc aagtttatca
     5521 ttaaaatcag aattagctta taaactaata gatgctgatg tagaaaaagt aaaagctgaa
     5581 ttattagcaa ttaataaatt atctcgtgaa tcattgaaca aagttcgaga aattattgat
     5641 gatgtaaaat taccatcatt tattgaagag attgatagta tacgtaaagt tttaaaagat
     5701 gctgatattg attttacatt tgaaaataaa gaattagcgc aagtattaag tcctactaaa
     5761 caatctatgt tagttatgat tacgcgtgaa gcgataaata atgttattaa acatgcaaat
     5821 gcttcaaaag ttcatggtaa attaaaaact gtaaacaatc ataaattact gcttatgatt
     5881 gaagatgatg gcaaaggtat cgatagtgat tgtgaggtga aaagtatttc acagcgtgta
     5941 caacatttaa atggaacttt agcagtcgac tcaacaaatg gaactaaaat aatcattgaa
     6001 atctcaacag gaggaatagc atgacatctt taattattgc agaagatcaa aatatgttac
     6061 gacaggcaat ggttcaatta attaaactac atggtgattt tgaaatttta gcagatactg
     6121 ataatggtct cgatgcaatg aaacttattg aagaatataa tcctaacgtt gtttttttag
     6181 atatagaaat gccaggcatg actggacttg aagttttagc ggaaattaga aaaaagcatt
     6241 tgaatattaa agtgattatt gtaacaactt ttaaaagacc gggatacttt gaaaaagcag
     6301 ttgtgaatga tgtggatgca tatgttttaa aagaacgttc tatagaagaa ttggtggaaa
     6361 ccattaataa agtaaataac ggagagaaag aatatagcgc cacattgatg acttcatttt
     6421 ttgtagataa aaacccatta acgcccaaag aacaaattgt attaagggaa attggcaatg
     6481 gtttaagtag taaagaaata agtgaaaaat tatttttgac agatggaaca gttagaaatt
     6541 atacatctgt tataattgat aaattatttg cagataatcg ttttgatgct tggaaaaagg
     6601 caaatgaaaa aggctggatc taaatacaaa caaaaaagta ttgagtgttt tgatttaaga
     6661 gacttggaaa gtgagtcaat aatcaaaatc ttactcaata cttttatttt ggtactcgca
     6721 atttagattt gttaactaat tttttcgctt attataaaac gaaacaatca agtaaacgat
     6781 aaagcctaca aagataccca ataaaataga tagtactgcc gtcactatta atggtaattt
     6841 gaaaaatatt tgtaggaaaa taccaatgat aattgcgata attactgcaa ttaatgtgac
     6901 tgtattttca tttgaaatgt tcatttattt tcactcctta acaataacat tatatcatgc
     6961 tatagctttc caaaatattg aaatatgtag atatggctat tgacgatatt tcttaacttt
     7021 tatatgatta atcggaatga aaaaagagaa gtaggtggca atatgaagtc aaataaatcg
     7081 cttgctatga ttgtggtagc catcattatt gtaggtgtat tagcatttca atttatgaat
     7141 catacgggtc ctttcaaaaa ggggacgaat catgaaactg tacaagattt aaatggtaaa
     7201 gataaagtac atgttcaaag agttgtggat ggtgatacat ttattgcaaa tcaaaatggt
     7261 aaagaaatta aagttaggct tataggggtt gatacgccag aaacggtgaa accgaatacg
     7321 cctgtacaac catttggcaa agaagcatca aattatagta agaagacatt aacaaatcaa
     7381 gatgtttatt tagaatatga taaagaaaaa caagatcgct atggtagaac attggcgtat
     7441 gtatggataa gtaaagatcg tatgtacaat aaggaattag tggaaaaggg acttgctaga
     7501 gagaagtatt tttcaccaaa tggcaaatat agaaatgtat ttatagaagc acaaaataaa
     7561 gctaaacaac agaaattaaa tatttggagt aaataattaa ataggtgatt atatatatta
     7621 aagtaaaata tacttgaagc atcgatttat tattaccata aatttatata tagaaataat
     7681 ttaaaggcat aataaccata gtgcgtatcg agcgtaatgg attattatgc ctttgatttt
     7741 acttgaatat agctgtgatt tgatcaacct gttctttatt taaagggtgt ttagatagct
     7801 ctatggcttc gctaataata cgtcgtaacg aagattttgt tgaaactgta tcaacatatt
     7861 tatctctttc ttcagaacct tgaatgatat taactgtacc atcactaaag taaataacac
     7921 cggtactttg gactttaaag ttaaagtttt gttcaatatg atcctttaat gctttcgcat
     7981 tatttgctgc taattgatac ggatcataat cataaaaatc ataaataaca cgatttggtt
     8041 gaactgtttc tgtaaaagta tagatagact ttcttgatga attaaactga tcatgatatt
     8101 gctggctaat gtaatgaccg acaacttttt caatattact attgcttatt tctgtatcat
     8161 gctcatcagg tacatcaaag tgataaaatg ttttttcacc ccagcttttt acatcaacgt
     8221 ttattaaacc gatatctgaa acaatgataa aatcaaatga acgtgcatac tcgaaaaacg
     8281 gatgtttagt agctaaattg cttgtaacga taatatcgta atattttaat ttaccatttt
     8341 cgagatagct atctaaaatc ttgcgtaatt ctcttttagc atttttgtat gcatggtggc
     8401 caacatcatt attagaactc agcatttgac ttcttaattc agcattttct gcacttaatg
     8461 ctttgttctt tttaataagt tgctttcttg cataaacttc ggtatctatt ttactattac
     8521 tatacctttg atttaaaact aatataccaa ttaatgctac aatgataatg ataagtacaa
     8581 cataaaaaga cattttttca ccaatccttt ttgacttctt taactttgta tacaataata
     8641 attaataaag attaattgtt attcaatttc ccacattttt attagttgat tttagttcat
     8701 cattgttata atcaaattat aaactgacag atattgatgt tcaatgaata tgacgtgaaa
     8761 gattcgtgaa ttcaagttta tgtcgaattt atgttataac ggtcatttaa atgacagaat
     8821 taggtcactc atagtatttt gaagattgaa ttcattaatt ttaaaatgta taatgatatt
     8881 tgtgaaagcg cttgcttagg aggtgtattt gagagtgaat gaaatgaatg ctaaagaaca
     8941 attagtggac aatttaatga aaacatcatc gcaattattt aaatttcacg gtgaagttgc
     9001 catgcagctt ttcttaaatg atgaattaaa attaccttct attgttgaaa tatgcgtgga
     9061 acgtaagcgt ttaagtgata ttgtgaaagt tattccgcaa tcatatgcgt tactatacat
     9121 agataagcaa gatcaagcaa tagctaaaga agatttatca ctttcaaaaa ttgcaaaagt
     9181 ttatgtgcaa tatgatgata caacaataat gagtattttc gtttatgatg tagtaaacga
     9241 tgaatggatt tttagattgg atccgaatat acgtatacct aagagtaaca tatacttcca
     9301 tagtttaaat tgggatgtgg attatattaa accggagatc gttctaatgt atgatctaat
     9361 gcaacaccat cagtatcatc attattccaa ttataaacga gtcatagatg cattaagcta
     9421 ctatcaattt tttattttaa aatttgtagt aggtgagcaa cgtattaagg atgcaatcca
     9481 gagaacaata aataattaag aaaaagcaat tcataacgca gttgaataca tgtgttacga
     9541 attgctttta tattagtttt tatcacacaa gttttttaat gcaaccccgt gatagcaaaa
     9601 ctcatatgta gataatacag ctttttcagc atcatctaca tgaattccaa acatcatcga
     9661 gatttccgaa gcaccttggt taatcatttt taagttaatt tttgattcag ctaaggcatg
     9721 tgtaatttta tttgcagtac cgatgacttt attcatacct tcgccgacaa tcattaatat
     9781 tgctaaatca tgctcaatac ttagctcatc aacatcacat ttttgacgaa tttcatttaa
     9841 tacttttgtt tctttatttt gaatttgttt tgaacgcata acgatactga tagtatcaat
     9901 acctgaaggc atatgatcaa atgaaatatt attatcctct aagacaccta atatctttct
     9961 agtaaagccg acttgtctat tcattaaata ctttttgata ttaataacgg taaaatcttt
    10021 atcacaactt ataccgctaa tcacattttt cgcatttatt tctctatcat gcactataaa
    10081 tgtaccctta tcttgagggc ggttcgtatt tttaatcacc acagggatgc gatctttata
    10141 aagtggttgt aaggcttcat catggaaaac actaaaacca gcataagata attcacgcat
    10201 ttctcgataa gtgatttctt cgattaattc aggatctttg atgatatttg gattagcttt
    10261 ataaatacca gacacatcgg tgaaattttc ataaattgtg gctctaacac cacttgatat
    10321 gatggcgcca gttatatctg atccgccacg tggaaatgta actatatatc cttcatgaga
    10381 tacgccaaaa aatcctggga taattagttt ttcatcataa tctcttaatt ttttaatttc
    10441 agagtaagca ctatctaata tttgtgcttc ttgtgggacg tcagtaacaa aaatacccgc
    10501 ttccttcggt gatatatatt ttgttggtat accttgacta ttattatata aagctatcaa
    10561 ttgcgcatta aaatcttcac cacaagaaag taatgcatct aatagtctct ttggttcatt
    10621 ttttaattga ttaatataat gttccaaagt cacatctatc gtccgtaaaa tactttcatc
    10681 catttgcaat tcttttacaa tatcatcata acgctgaata atttctcttt ttttatcatg
    10741 ataatcaaga tgattaatga ccttttcata taatctgatt aacaaatcag ttgttttaat
    10801 atcattatca tgtcttttac ctggagcaga aacgataaca atctttcgct ctggatcaga
    10861 attaacaata tttaaaacct ttttaatttg agtagcattg gagacggagc taccaccgaa
    10921 tttggaaact ttcatagtgc atgccaacct ttctaaaaaa tcagaaaatt gtattcagtt
    10981 ttgtaagtaa gaatgatatt atgtatttat attttgaata ttacgaactt tacaaaaata
    11041 aagaataatt ctatactata ctatcatcta gtgtttttca ataatacatt tgtttttctc
    11101 aaaagcacaa acggaggtac ataaaatgaa aaaattaaat atagcattat taggattagg
    11161 tactgtcgga tctggtgttg ttaaaatcat cgaagagaac cgacagcaaa ttcaagatac
    11221 attaaataaa gatattgtca taaagcatat tcttgttcga gataaatcta aaaagagacc
    11281 gctaaatatt agccaatatc atttaactga agatgttaat gaaattttaa atgatgattc
    11341 attagatatt atcgttgaag tcatgggagg aattgaacca actgtagatt ggttaagaac
    11401 agcacttaaa aataaaaaac atgttattac cgcaaataaa gatttattag cagtacatct
    11461 taaactttta gaagatttag cagaagaaaa tggtgtagct ttaaagtttg aagcgagtgt
    11521 agcaggtggt attccgatcg taaatgccat aaataatggt ttgaatgcga ataatatttc
    11581 aaaatttatg ggaattttaa atggtacctc taattttatt ttatctaaaa tgactaaaga
    11641 gcaaacgaca tttgaggaag cacttgatga agcgaaaaga cttggttttg ctgaagcgga
    11701 tccaactgat gatgtagaag gggtagatgc agcgcgtaaa gttgtcatta catcatattt
    11761 atcatttaac caagtcatta aattaaacga cgttaaacga agaggaatta gtggcgtaac
    11821 tttaactgat attaatgtag ccgatcaact ggggtataaa attaaattga ttggtaaggg
    11881 aatatatgaa aatggcaaag ttaatgcatc ggtagaacca acgttaattg ataaaaagca
    11941 tcaattagca gctgtagagg atgaatataa cgcgatttat gttattggtg atgccgttgg
    12001 tgacacgatg ttttatggaa aaggagcagg cagtttagca acaggtagtg ccgttgtcag
    12061 tgatttattg aatgtagcat tattctttga atcagattta cacacattgc caccacattt
    12121 tgaattaaag acagataaaa cacgggaaat gatggattca gatgcagaaa ttaatattaa
    12181 agaaaaatcc aatttctttg tagtagtgaa tcatgtcaaa ggttcaattg aaaattttga
    12241 aaatgagtta aaggcaatat taccatttca ccgatcatta agagttgcaa attacgataa
    12301 tcaatcatat gccgctgtta tagttggatt ggaatcatca ccggaagaat taatcactaa
    12361 gcatggatac gaagttgaca aagtataccc agtagaagga gtttaattat aatgagaaga
    12421 tggcaaggat tagtagaaga gtttaaagca catttaccag taaatgaaaa tacaccaaaa
    12481 ttaacattga acgagggaaa tacaccactc attcattgtg aaaatatgtc taaaatacta
    12541 ggcatagatt tatatgtgaa gtatgaaggt gccaatccga caggttcatt taaagatcgc
    12601 ggtatggtaa tggctgtgac aaaagcaaaa gagcaaggta agaaaattgt aatatgcgct
    12661 tcgactggaa atacatcagc gtctgcagca gcatatgcag cgagagcagg tttaaaagct
    12721 atcgtcgtaa taccagaagg taaaattgca ttaggtaaat tgtcgcaagc agtaatgtat
    12781 ggtgcagaaa tcgtttctat tgaaggaaac tttgatgaag ctttagaaat tgtaaaagaa
    12841 attgcaaaaa gtggcgaaat cgagcttgta aactctgtca atccatttag aatcgaagga
    12901 caaaagacag gctcatttga aattgtacaa caattagacg gtgaagcacc tgatatttta
    12961 gcgattcctg taggtaatgc aggtaatatt actgcatatt ggaaaggctt taaagaatat
    13021 catgaagcta aaggatcaca attgccgaaa atgtttggct tccaagctga aggcgcatca
    13081 ccaattgttc aaaataaagt cattaaaaat cctgaaacga ttgcaactgc tattcgaatt
    13141 ggtaatcctg ctagttggga taaggcgact aatgctctta aagaatcaaa tggattaata
    13201 gatagtgtta ctgatgatga aattctagaa gcatatcagt taatgacaac taaagaaggt
    13261 gtctttagtg aaccagcgag taatgcttct attgcaggtt taattaaatt gcatagacaa
    13321 ggtaaattac ctcaaggtaa aaaagtagtt gctattttaa ctggtaatgg attaaaagat
    13381 cctgatactg ctatttcact actagataat ccgataaagc cattgccaaa tgataaagat
    13441 agcattatcg attatattaa aggagcttta taacatgtcg aatgttttgg agttaacaat
    13501 tcctgcatca acagccaacc ttggagttgg ctttgattct ataggtatgg ctttagataa
    13561 atttttgcat ctgtctgtaa aggaaacatc agggacaaaa tgggaatata ttttccatga
    13621 tgatgcatct aagcaattgc ctactgacga aacaaacttt atttatcatg tagcacaaca
    13681 agttgcttct aaatatagtg ttgacttgcc taatttatgt atcgaaatga gaagtgatat
    13741 tccattggca agagggttag gttcgtcagc ttctgcttta gtaggagcta tatatatcgc
    13801 aaattatttt ggtgatatcc aactgtctaa acatgaggta ttacaattag cgactgaaat
    13861 cgaaggacat cctgataatg ttgcgccgac catttatggt ggtttaatcg ctggatatta
    13921 taatgatgtc tcgaaagaaa cgtcagttgc acatatcgac ataccagacg tggatgtgat
    13981 tgtaacgata ccaacttatg aactaaaaac agaagcatca agacgtgctt taccacaaaa
    14041 attaacacat agtgaagcgg ttaaaagtag tgcaattagt aatacaatga tttgtgcatt
    14101 agcacagcac aattatgaat tagcaggtaa actcatgcaa caagatggct ttcatgaacc
    14161 gtatcgtcag catttaattg ctgaatttga tgaagtgaaa acaattgcta gtcaacataa
    14221 tgcctatgca actgtaatta gtggtgctgg accaactatt ttaatattta gtcgtaaaga
    14281 aaatagtggg gaattggttc gctctttaaa tagtcaggta gtatcatgcc attctgaatt
    14341 ggtcgatatt aatatcagtg gtgttaaaga acgaattgta taccaataga cgctttatat
    14401 tgtaaaatag tattaaatgc agaatagaga ggagatttaa tgcgatatga caaattataa
    14461 agttgtcgtt ttagatatgg atgacacatt gctaaattca gataatgtga tatcagaaga
    14521 aactgcaaat tatttaacag caattcaaga tgaaggttat tatgttgttc tagcatctgg
    14581 tagacctact gaaggtatga ttccaactgc tagagattta aaattacctg aacatcatag
    14641 ctatattatt agttataacg gtagtaaaac gattaacatg actaatgaag aagtagaagt
    14701 aagtaaatcg attggtaagc aagatttcga tgaaattgta gattattgtc gagatagagg
    14761 ctttttcgtt cttacatatc atgatggtca aattatttac gacagcgaac atgagtatat
    14821 gaatattgaa gcagaattaa caggtttacc gatgaaacgt gttgatgata tcaaagcgta
    14881 tattcaaggc gatgtaccca aggtcatggg tgtagattat gtagcgaata ttacagaagc
    14941 tagaattgat ttgaatggtg tgttcaatga taatgtagat gctacgacaa gtaagccatt
    15001 cttcttagaa tttatggcca aagacgtttc aaaaggtaat gcaattaaag cgttatgtca
    15061 caaattggga tattcggtgg atcaagtcat tgcttttggt gatagtatga atgataaatc
    15121 aatgtttgaa gtcgcaggtc tagctattgc tatggggaat gcatcagatg aacttaagca
    15181 atatgcaaat gaagttacgt tggatcataa tgaaaatggt attccacatg cgctcaaaaa
    15241 attgttataa attttaaaat aagccttaac acatgatatt tgaataagat atcttgtggt
    15301 taaggctttt tatttttgtg aaaatgactt cagttatact atggaggatt tgaaatacat
    15361 attttagatt agtaatgata tcaaacgaat agagtaaatg tatatttttt gaataaatca
    15421 agtattaact agtcacggaa ggtagataaa taaatttgat cattctgaat aataacgaat
    15481 gcattaaaaa atgcagtgac aatctaacat taattgttaa aatcactgca agcatcttga
    15541 ttttatttag ctgtttgtgt ccagtcatgc ttacctctga aacgttttac atgagcatat
    15601 atttggtcag cagtataatc taaatttgta taaactgtaa tactgaacgc tgattggttt
    15661 tcagcaaatg attcttcgga aaataattcc tttaagtgca taaataaatc attcatttgt
    15721 ttatggttta aaccgggata ttcccttaaa gttctcttta ataattgtcc gttcttttct
    15781 tctaatgctt gaactacaat aacaaatctt tcatcttgct ttagtacatc atcaaataaa
    15841 tttgtttgtc caatcattga atttcacccc tgaaaagtta ttgttatcta tattatacac
    15901 gatatgcaaa atatctaaaa taaagaatgg taacatggta ataataaaat ataaacagaa
    15961 tactagttaa tacactttat actagaaaaa gatattaaag tatatctgct ttacaccatt
    16021 aaaaagcggc aaatgctata gataatctaa agcatctgcc gtatgagaat ttatttattt
    16081 ttgattgtca taatcgtgtg gttttaaatt aattgtttct agctttacaa attttgtttt
    16141 gtgaatgatt ttatgaataa agtaaatcaa cgctagaatg attaaaggta aaaagttttt
    16201 aaaagcattt aaccattgat cttttaaaat atattcaact gagccaccaa atagcaagaa
    16261 taatagtgta gtgatgacaa tgattggtcc taatggataa aaaggtgctt tatatggtag
    16321 gaccttatta gggtcttgac cttgtttttt aatagcttgt cgcaatcgta tttgtgacca
    16381 aatgcttgat ccccaaacaa ctataatcat tgaaccaata atttcaagta aattaaaaac
    16441 ggcatttgaa ttaaagtttg cataaataat aacaataaca acgactgcat aagtagttaa
    16501 taatgctctt aaaggtaact tagttgtctt gtttaattta cttaaaaatt ggggtgcttt
    16561 tttgtctgaa cttaaggaat acaacattct gcctgttgta taaacacctg aatttgcagc
    16621 ggataataat gaagttaaaa taaccgcgtt gattactgat gctgcaaagg ctatgcctac
    16681 tctatcgaat acaattgtaa atgggctttg acttattgaa ctacttgctc ttaataatga
    16741 tggatctgtg tacggaataa ttgcaccaat tactgcaatt gataagacat agaataaaag
    16801 aatacgccaa aatacttgtt taattgcctt aggcatagac tttttagggt catctgattc
    16861 accagcagtt actgctacta cttctgtacc accaaccgaa aatccggcga ctaataatac
    16921 gcctaagaaa ccagagatac caccaacaaa cggtgcttgg ccttttgtat agttttcaaa
    16981 tccatatgta tgaccaccta agataccgaa aatcattaaa aagccaaaaa taacgaatac
    17041 gataattgtt aacactttaa tcaatgataa ccaaaactca gtttctccaa atgattttac
    17101 agaaaaaatg tttaataata gtaaaattgt aataaagatt aagctccaag taatggggtg
    17161 gaaaaattta aatgtgtccc agaaataaag cacatttgac gctactatga catcaacact
    17221 tgtaactaat gaccacaatg cccaatacaa ccatcccatg gtaaagccaa gagatgagtc
    17281 aataaagcgt gttgaataag agctgaatga acctgatact ggataaaatg ttgccaactc
    17341 tccaattgat gacattaaga aatatagcat gacaccaata acaagataag cgagtatagc
    17401 gcctccagga ccagcttgag aaatgatatt accagtagct acaaatagac cagtcccaat
    17461 tgcaccacct atagcaatca tggaaatgtg tcttgagtta agactacggt tcattttatt
    17521 atcttccata tttagtctcc catctattta aatataccca ttattgtaag ctttttaagt
    17581 gtactattca ataactattt agtactgtaa agcgaaaaaa ttaaaatttt ctgatttttt
    17641 aatcatcttg agcatgttta attgtaattt tgatggggtt aaattataat atgtattaaa
    17701 ttataattat tataaattgt ggagggatga ctatgtcaca acaagacaaa aagttaactg
    17761 gtgtttttgg gcatccagta tcagaccgag aaaatagtat gacagcaggg cctaggggac
    17821 ctcttttaat gcaagatatt tactttttag agcaaatgtc tcaatttgat agagaagtaa
    17881 taccagaacg tcgaatgcat gccaaaggtt ctggtgcatt tgggacattt actgtaacta
    17941 aagatataac aaaatatacg aatgctaaaa tattctctga aataggtaag caaaccgaaa
    18001 tgtttgcccg tttctctact gtagcaggag aacgtggtgc tgctgatgcg gagcgtgaca
    18061 ttcgaggatt tgcgttaaag ttctacactg aagaagggaa ctgggattta gtagggaata
    18121 acacaccagt attcttcttt agagatccaa agttatttgt tagtttaaat cgtgcggtga
    18181 aacgagatcc tagaacaaat atgagagatg cacaaaataa ctgggatttc tggacgggtc
    18241 ttccagaagc attgcaccaa gtaacgatct taatgtcaga tagagggatt cctaaagatt
    18301 tacgtcatat gcatgggttc ggttctcaca catactctat gtataatgat tctggtgaac
    18361 gtgtttgggt taaattccat tttagaacgc aacaaggtat tgaaaactta actgatgaag
    18421 aagctgctga aattatagct acagatcgtg attcatctca acgcgattta ttcgaagcca
    18481 ttgaaaaagg tgattatcca aaatggacaa tgtatattca agtaatgact gaggaacaag
    18541 ctaaaaacca taaagataat ccatttgatt taacaaaagt atggtatcac gatgagtatc
    18601 ctctaattga agttggagag tttgaattaa atagaaatcc agataattac tttatggatg
    18661 ttgaacaagc tgcgtttgca ccaactaata ttattccagg attagatttt tctccagaca
    18721 aaatgctgca agggcgttta ttctcatatg gcgatgcgca aagatatcga ttaggagtta
    18781 atcattggca gattcctgta aaccaaccta aaggtgttgg tattgaaaat atttgtcctt
    18841 ttagtagaga tggtcaaatg cgcgtagttg acaataacca aggtggagga acacattatt
    18901 atccaaataa ccatggtaaa tttgattctc aacctgaata taaaaagcca ccattcccaa
    18961 ctgatggata cggctatgaa tataatcaac gtcaagatga tgataattat tttgaacaac
    19021 caggtaaatt gtttagatta caatcagagg acgctaaaga aagaattttt acaaatacag
    19081 caaatgcaat ggaaggcgta acggatgatg ttaaacgacg tcatattcgt cattgttaca
    19141 aagctgaccc agaatatggt aaaggtgttg caaaagcatt aggtattgat ataaattcta
    19201 ttgatcttga aactgaaaat gatgaaacat acgaaaactt tgaaaaataa atttgatatg
    19261 tagtttctat attgcgtagt tgagcagttt atgatatcat aataaatcgt aaagattcct
    19321 aacaagagag ggtgtttaac gtgcgcgtaa acgtaacatt agcatgcaca gaatgtggcg
    19381 atcgtaacta tatcactact aaaaataaac gtaataatcc tgagcgtatt gaaatgaaaa
    19441 aatattgccc aagattaaac aaatatacgt tacatcgtga aactaagtaa ttcttatcat
    19501 tcaaatacga cgatttgaaa ataaagcggg cttacctatt atattgggga gctcgctttt
    19561 ttatgaaatt tttgtgaaga gtgattaatg gattgagttt catcggtaga acaatatatg
    19621 attatattag ttgttacttt attaaaattt gagaatattt atagaaggaa atagattact
    19681 gattttataa agtcactttg ttagcgaatg cttgaaagag tatttaatat agtagaattt
    19741 aaaatttcaa agcggaattt aataagtacg aagtaattct gggtatgttt tataaatgtt
    19801 cgataataca ctttaatctt aaatatgatg gtttagaaaa tgatttaaca aagaaatgaa
    19861 actttactgt tgaattatgt gaggattgtg ttatt
//
LOCUS       DANJFZ010000044        19506 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.044, whole genome shotgun sequence.
ACCESSION   DANJFZ010000044 DANJFZ010000000
VERSION     DANJFZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.044"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="PWN45_001367"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="PWN45_001367"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDL0623137.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="PWN45_001368"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="PWN45_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDL0623138.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="PWN45_001369"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="PWN45_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDL0623139.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="PWN45_001370"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="PWN45_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDL0623140.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="PWN45_001371"
     CDS             complement(6315..6440)
                     /locus_tag="PWN45_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623141.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="PWN45_001372"
     CDS             6606..7589
                     /locus_tag="PWN45_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0623142.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="PWN45_001373"
     CDS             7601..8632
                     /locus_tag="PWN45_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0623143.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="PWN45_001374"
     CDS             8629..9597
                     /locus_tag="PWN45_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0623144.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9833..11203
                     /locus_tag="PWN45_001375"
     CDS             9833..11203
                     /locus_tag="PWN45_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDL0623145.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11335..11595)
                     /locus_tag="PWN45_001376"
     CDS             complement(11335..11595)
                     /locus_tag="PWN45_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623146.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11772..12287
                     /locus_tag="PWN45_001377"
     CDS             11772..12287
                     /locus_tag="PWN45_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0623147.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12331..13518
                     /locus_tag="PWN45_001378"
     CDS             12331..13518
                     /locus_tag="PWN45_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDL0623148.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13537..14220
                     /locus_tag="PWN45_001379"
     CDS             13537..14220
                     /locus_tag="PWN45_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL0623149.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14397..15740
                     /locus_tag="PWN45_001380"
     CDS             14397..15740
                     /locus_tag="PWN45_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623150.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            15839..16312
                     /gene="sepA"
                     /locus_tag="PWN45_001381"
     CDS             15839..16312
                     /gene="sepA"
                     /locus_tag="PWN45_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDL0623151.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            16634..18076
                     /gene="lmrS"
                     /locus_tag="PWN45_001382"
     CDS             16634..18076
                     /gene="lmrS"
                     /locus_tag="PWN45_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDL0623152.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18224..19288
                     /locus_tag="PWN45_001383"
     CDS             18224..19288
                     /locus_tag="PWN45_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDL0623153.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19377..>19506
                     /locus_tag="PWN45_001384"
     CDS             19377..>19506
                     /locus_tag="PWN45_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623154.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFL"
BASE COUNT         6427 a         2658 c         3587 g         6834 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaat tacaagaaat agcgtatatt taataactgt aattattcat
     9721 tattttgcat acataaacat tttgaaagat agaatgataa ggaatatgtt tgttaactga
     9781 atagaattct gtatatactt aaaacgaata catagagaaa tggattgatt ttatggatag
     9841 cttttcaagg aaagagatag ttattgggag acttaagttt attacgatgt ctttaattgg
     9901 tatcttatta tttttagtac caattccagt cgaacaagat ggacagaagc aaacaacgtt
     9961 gcccgtggca tttttagcag gcgtgcttaa agatgtatta ggtggcgtaa tgccattttt
    10021 aattgtgacg ataattactt tatcgggtat cataactctg atttgttcaa ctattttaaa
    10081 agataaattg aaacctgacg ggctaatgaa caatgctttt aacgttagaa ttggttggtt
    10141 aattttacga atattagcag ttgtttttgc atggatgaca tttttacgaa ttggttcaaa
    10201 ggttatttac tcagatgaaa cgggtggatt attgttctca agtttattgc caacattagt
    10261 ggcagtattc ttatttgcag ctctattttt accgctatta atggaatacg gtttgttaga
    10321 gatgttgggt cctattttta gaccggttat gagaccttta tttacattgc caggacgttc
    10381 gacggtagat aatttagcat catttattgg agacggaact gttggtgtct taattacaag
    10441 tagacaatat ggtgaaggat attattctcg tcgtgaggca acagttattt caacgacatt
    10501 tagtgtagtt tctatcacct ttgcaattgt agttgctgaa acagtgcaca tgcaaaatca
    10561 attttttgct ttctatttat cagtgatagt atcctgcctc gttgcagcag tcattatgcc
    10621 aagaatttgg cctttaaata aaattcctga tgagtatgct aaggaggtac ctgaaagtgc
    10681 gcgtactgag gcattgccag aaggaaagac ggcattgaga catggctttg atactgcaac
    10741 agaagtaggt attaaagcac cgggcgttat tgactttttt aagtcaggat taaaaacagt
    10801 cattgatatg tggtttgtta ttttgcctgt agtaatgagt attggtacta ttgctaccat
    10861 tattgcgaac tacacgccct tttttgtcat attgggtaaa ccttttgttc catttttaga
    10921 gttaatgcaa atacctgaag cggcacaggc ttctcaaacg attattatcg gttttgccga
    10981 tatgttttta ccttctatat taattgaagg tgtacaaaat gatataactc gttttgttat
    11041 tggggcactt agtatatcgc agttaatata cttgtctgaa gtaggtggcg tcatacttgg
    11101 ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt ttcttgatta gaacaatcat
    11161 tacactacca attatttctt tgatggcaca tcttttgtta taaatatatg tgaaaaagga
    11221 caactgttaa aattatggga acagttgtcc ttttgtattt gtttatgctg ggtgttgaat
    11281 caattaaaaa aatcattcat gaagccctca ccatgaatga tttttttata caatttattt
    11341 agttaattca ttgtaaactt tttgatcatt ttgagtataa ataatgtatt cgtggtcttc
    11401 agtatcgata ataacgcgat tcgttttacc aaacgttgag ccgattctag atacatcttt
    11461 gctgtctaat ttaggaactg catgaatatc ttggtcttgt gtaatatttt taatttcact
    11521 tgtaggaatt ttgatatcag caactctcca ttgaatacgc acttcattat tatccttttt
    11581 tactgtcatt gccattattc aaacacatcc tttaaatatt ttgtaaatac atcataaacg
    11641 attttgtaat cgttttcaag agttatgtga tactttttta aattgtgtca tactaatgaa
    11701 ttttaacccg aaaatatgtt cgtgtttttg ttagggatgt ggtaaaatgt gagtattaaa
    11761 ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg catgctcgtt ggagcgctaa
    11821 caacacaata ttttcaaacg gatatttttt cgtcggttac ggttatcatt ctagcaactc
    11881 tagctagctt attacccgat atatgtcaca ctcaaagtaa gattggcaga aggtttaagg
    11941 taattagttt ttttgtgaga ttgatctttg gacatcgaac ctttacgcat tcaatattat
    12001 ttatcgcaat catagcattt ttattgcaaa ttattcagac gcctaattat tatatggcag
    12061 caattataat tggattagtg tcacatgtta tactagatat gataacacca aaaggcgtga
    12121 aattatttta tccgttaccg tttaatgtga agacaccgat tcaatttaaa acaggtggac
    12181 ttgtagactt atctttagct acagcgttaa tggtcggaac agtttatgtt ttatttcagc
    12241 ctttcatcaa tgatataata agcaactgga atactaagtt tttatagaaa ataaattatt
    12301 aaaggttaca aactcagcag gaggcataaa atgctagata aaaatcaatt agctaaatat
    12361 aaacaagatc atttgtgtga atatgaaaaa ataatgagta acaatgaaaa agaagcactt
    12421 gaagagaagg tagcgtcatt agatttagat tttattgcca aattatataa cgatttatac
    12481 atcaataaga aaacaataga tgatgtatct gcagtttctg aagtgaaata tgatattaaa
    12541 tcacaaatga gtgatgatga gattaagcgt ttagaggaac aaggacttca agctattaaa
    12601 gaaggacaat ttgcagtact tttgatggca ggtggtcaag gaacaagact tggttacaag
    12661 ggccctaaag gttcgtttga aattgaaggt gtaagtttat ttgaacttca agctaaccaa
    12721 ttaaaaacat tgaatcatca atctggacat acgattcaat ggtatattat gacaagtgat
    12781 atcaatcatg aagaaacttt agcttatttc gaagcacata gttattttgg atatgatcaa
    12841 gaagcaatcc atttctttaa acaagataat attgtggcgc ttagtgaaga agggaagtta
    12901 attttaaatc aacagggtcg tattatggaa acgccgaatg gtaatggtgg cgtgtttaaa
    12961 tctttagaca aggcaggata cttagaagaa atgtctaata atggcgttaa gtatattttc
    13021 ttgaataata tcgacaatgt tttagtaaga gtgttggatc cactatttgc aggatttacg
    13081 gtagaacatg attacgatat tacttctaaa acgattcaac caaaaccagg agaaagtgtt
    13141 ggacgtttag ttaatgtcga ttgtaaagat acagtactgg agtattcaga attagatcca
    13201 gaagttgcaa atcaatttaa caatgctaat atcggtattc atgcatttaa acttggtttt
    13261 attttaaacg ctgtaaatcg tgagttacct tatcatttag ctattaagaa tttgaagcaa
    13321 ttagatgaga actttggtgt tattgaacaa ccaactttaa aattcgaatt gttctatttt
    13381 gacatattta cttatggaac tagttttgtc acgttacaag ttcctagaga agaagaattt
    13441 tcacctctta aaaataaaga aggtaaagat agtgttgcaa ctgcaacaga agatttacgt
    13501 agaatgggtt taatttaatg ataggcggtg actatgatgt ccaaatcaac taaaaaaagt
    13561 aatgacacaa tcgtagaaac atttaaggat atcatccctt tatcatttgg agaagaaatt
    13621 ggtaatgcgg catctcatgg tgttgcagct ttgttaacat tattagtgtt accctatgcg
    13681 gctgtgcaca gttatattgc acatggtacg cttgcgtctg tgagtatgtc tatttatgtt
    13741 atttctatct ttatgatgtt tatttcatct actatttatc attctatgca aaatgaaaca
    13801 cctcataaat atattttaag gattatcgat cacagtatga tttacgtagc aatttctggt
    13861 acatatacgc ctattttgtt aacagttgta ggtggttgga taggttggac agtatttatt
    13921 ttattatggg gaacgacact ttgggggatt ttatataaat ccatagctgt caaagttaac
    13981 caaaagttaa gcttaattgt ctatttagtg atgggctggg taggtattat cttgttacca
    14041 attattttta ttcgcacatc atggatattc atattattca ttttcttagg tggtgtgtct
    14101 tatacaattg gtgcatggtt ttatgcacaa aagaatagac catattttca tatgatttgg
    14161 catattttta ttgtaatagc atcactattg catttaattg caatcttata ttttatgtag
    14221 tcgtttagaa aagctggggg aaactccgct ttttttatta cataaatcat tctaataggg
    14281 gattgtattg taatagaatt atcgttaaga tttgaaagtc aaaagtgttt atacatatga
    14341 aaaccaatta tttgtaaata aaatcttata aataaatata tagaaggagg tatttcatgc
    14401 gattaaagtc aatcataact gttatagctt taatattaat tatgtttatg tcagcgattg
    14461 aatcatctat tatttcgctt gcattaccta caattaaaca agatttaaat gcaggaaatt
    14521 taatttcatt aattttcaca gcgtatttta tcgcattagt aattgccaat cctatagtgg
    14581 gcgaattact ttcgagattt aaaattattt atgtggcaat tgcaggatta cttttattta
    14641 gtataggtag ttttatgtgt ggtttaagta ctaattttac tatgctaatt atatcacgcg
    14701 tgatacaagg ttttggatca ggtgtattga tgtcattatc gcaaattgtt ccaaagttag
    14761 catttgaaat tccgttacga tataaaatta tgggcatagt tggcagtgtt tggggtattt
    14821 cgagtatcat tgggccactt ctaggtggtg gtatattaga atttgcaaca tggcattggt
    14881 tattctacat aaatataccg attgcgatca ttgccattat attagttata tggacatttc
    14941 attttccaga ggaagaaaca gttgctaaat caaaatttga tactaaaggg ctcacgctat
    15001 tttatgtatt tattggattg attatgtttg ctttattaaa ccaacagctg ttacttttaa
    15061 atttcttgag ttttatctta gctatcgttg tggcaatgtg tttatttaaa gtagaaaaac
    15121 atgtttcttc accattttta cctgtggttg aatttaatcg ttcgattact ttagttttta
    15181 taactgacct tctaacagct atttgtttaa tgggattcaa tttatatatt ccagtctacc
    15241 ttcaagaaca actaggatta tctccattgc aaagtggatt ggttattttt cctttatctg
    15301 tagcttggat tacattgaat tttaatttac atcgaattga agcaaaacta tcaaggaaag
    15361 ttatttactt actatcattt acattgctac tagtaagtag tattatcatt tcatttggta
    15421 ttaaattgcc ggtacttata gcatttgtgt taattttggc aggattaagt tttggatata
    15481 tttatacgaa agatagtgtg attgtccaag aggaaactag cccattacaa atgaagaaaa
    15541 tgatgtcatt ttatggatta actaaaaatc ttggggcatc aataggttca acaataatgg
    15601 gatatcttta tgcgatacaa tcaggaatct ttggtccaaa cttacacaat gtgttaagtg
    15661 ctgttgctgt aattagcatt gggcttattg ttttatgggt cgtatttttt aaagaacagt
    15721 catctcaatc aaaagaatag aattatgatt aaaattgatg tagtgtaatt aaagtaagaa
    15781 ataataaatt tcctagatac tagataaatc gttcaatatt ttattaataa ggaggccaat
    15841 gattgtgaat tatcttaaac ataagtttta taacttgtta actacaatga ttgttctctt
    15901 tatttttgta ctttctggtg cgattttttt aacatttcta gggtttggtt tatatggatt
    15961 aagtagaata cttatttatt ttaggttggg tgactttaca tataatagaa gtatgtacga
    16021 taacctatta tattatggca gttatataat ctttgggtac ttcatcatat ttgcagtcga
    16081 gcatttaatg gattatttta gaaaaatgtt gcctgaaaat gcatatttta gaggcgcgac
    16141 ttttcatttg atttcttaca cagttgcaac aacgttattt tattttatta ttcatctgaa
    16201 ctatgtgtat atcaatatcg atttttgggt catcatggtt attatagggt tcttatatgt
    16261 ttgtaagctt caattttacc ctgaaagtaa aaatttaaat aatagaaaat agatatctga
    16321 agttgagggc acatatgtgt tccaacttct ttttgttcaa tagaagaatc gataatagtg
    16381 agtcttagtg ctcaatttgt ggtaaataag ttaaataacc attgtatgag tctgagacat
    16441 aaaataaatt attaggccct acaatgttat attgttagtt tttaagtaca cattagctgt
    16501 gcctaatgtg taagaaccaa taatgattta ttatgtagtg cttctttatt atttgagtac
    16561 tactcccatt atgttcatgg ctatacgtat taaagggaca agtgtatatc tgttttaaaa
    16621 aggaggaatt atgatggcta aagttgaatt aacaacccgg agacgaaact tcatagttgc
    16681 tgttatgttg attagtgcgt ttgtagctat tttaaatcaa acattattaa atacagcgtt
    16741 acctagtata atgagagaat taaatatcaa tgaaagtaca tcgcaatggc tagttactgg
    16801 gtttatgctt gttaatggcg tcatgatacc tctgacggca tatctaatgg atagaattaa
    16861 aactagacct ttatacttag cggcgatggg gacattttta ttaggttcta ttgttgcagc
    16921 cttagctccg aattttggag ttttaatgtt agctcgtgta attcaagcga tgggtgcagg
    16981 cgtacttatg cccttaatgc aatttacgtt atttacattg ttcagtaaag aacatcgagg
    17041 ttttgcaatg ggactagcag gtttagtaat tcaatttgca ccagcaatag gacctacagc
    17101 tacaggatta attattgatc aagcgagttg gcgagttcca tttattataa ttttaggaat
    17161 tgctatactt gcctttgttt tcggtttggt ttcaatctcg agttacaatg aagtgaaata
    17221 tacgaaatta gataagcgtt cagtaatgta ttcaactatt gggttcgggt taatgctata
    17281 cgcatttagt agtgcaggag atttaggatt tacaagtcca atagtaatag gtgcgttgat
    17341 attaagtatg gttattatct atttatttat acgtagacaa tttaatatta ctaatgcact
    17401 tttaaattta agggttttta aaaatagaac atttgcatta tgtacgatta gttcaatgat
    17461 tataatgatg tcaatggttg gacctgcgct gcttataccg ctatatgttc aaaacagttt
    17521 atctttatct gccttgttat caggacttgt tatcatgcct ggtgcaataa taaatggtat
    17581 tatgtcagtt tttacaggta aattttatga taagtatggt cctagaccat tgatttatac
    17641 tggttttaca attttaacaa ttactacaat tatgttgtgt ttcttgcata cagacacatc
    17701 ttatacgtat ttaatagtcg tatatgcaat tagaatgttt tcagtttctt tactcatgat
    17761 gccgataaat actacaggaa ttaattcttt gagaaatgaa gaaatctcac atggcacggc
    17821 tattatgaac tttggtcgtg taatggctgg ttcactaggc acagctttaa tggttacatt
    17881 aatgagtttt ggtgcaaaaa tatttttatc tacatcgcca tcgcatttaa ctgcaactga
    17941 aattaaacag caatccattg ctataggggt ggatatctca tttgcttttg tagctgtgct
    18001 tgttatggca gcttatgtga tagcactttt tataagagaa cctaaagaaa tagaaagtaa
    18061 tagaaggaaa ttttaaaata attatagtag ttggtctatt taaaataata ggctaactgc
    18121 tttttttatt taataaaaag ttttatactt ttagtgatag actaagcaaa aattgttatt
    18181 tgctatgatg tagatgtctt aaaatgatta agggggattt gctttgttaa cggtagatca
    18241 agtgaaagaa ttggtaggag aaattaaaga tcctattata gatgtgcctt taaaagaaac
    18301 agaaggtatt gttgaagttt ctattaagga agaaaaagaa catgtgagtg ttaaattagc
    18361 aatggcacaa ttaggtggtg caccgcaatt agatttacag atggctgttg ttaatgcatt
    18421 aaaagaaaac ggtgcgaaaa cggtcggtat acgatttgaa acattgccgg aagaaaaagt
    18481 aaatcaattt aaaccaaaag aagaaaataa acctaaaacg atagaaggcc tattatctca
    18541 aaataatcca gtcgaattta ttgcaatagc ctccggtaaa ggtggtgtcg gtaaatctac
    18601 tgttgcagta aatttagccg ttgccttagc tcgtgaaggg aaaaaagtcg gattagtaga
    18661 tgccgatata tatggattta gtgtaccaga tatgatgggt attgatgaaa agcctggaat
    18721 taaagggaag gaagtaattc cagttgaacg tcatggcgtt aaagttatat caatggcctt
    18781 ttttgtggaa gaaaatgcgc cagttatatg gagagggcca atgttaggta aaatgttgac
    18841 gaatttcttt acagaagtta aatggggaga cattgaatat ttaatactcg atcttccacc
    18901 tggaacagga gatgtagctt tagatgttca tacgatgtta ccttcaagta aggaaattat
    18961 tgtaacgaca cctcatccta cagcagcatt tgttgcagct cgcgcaggtg cgatggcaaa
    19021 acatacggat cattctattc ttggcgtaat tgaaaacatg agttattttg aaagtaaaga
    19081 gacgggtaat aaagaatatg tctttggcaa aggtggcggt actaagttag ctgatgaact
    19141 taatactcaa ttacttgggg aattaccttt agagcaacca tcttggaatc caaaagattt
    19201 tgcaccgtca atatatcaat cagatgatcg tctaggtaaa atttatagct cgattgcaca
    19261 aaaagttata gctgctacaa ataaataggt ataatatatg ctaaacgtac aaaattactt
    19321 ttattttgta cgtttttatg ttgatttgtt ttggaatgct ggtaaaataa gttcttgtgt
    19381 taagcaataa acgcgttgaa aaagaagcgg gttgtgaaca caaaaagttt ttaaaaaagt
    19441 tattgacttt aaattccaaa gttgataata tgataaagtc gtcaaaaacg acgcagttgt
    19501 ttttaa
//
LOCUS       DANJFZ010000045        19237 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.045, whole genome shotgun sequence.
ACCESSION   DANJFZ010000045 DANJFZ010000000
VERSION     DANJFZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.045"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            274..648
                     /locus_tag="PWN45_001385"
     CDS             274..648
                     /locus_tag="PWN45_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDL0623155.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            801..1199
                     /locus_tag="PWN45_001386"
     CDS             801..1199
                     /locus_tag="PWN45_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0623156.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1415..2239)
                     /locus_tag="PWN45_001387"
     CDS             complement(1415..2239)
                     /locus_tag="PWN45_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL0623157.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2476..3783)
                     /gene="brnQ"
                     /locus_tag="PWN45_001388"
     CDS             complement(2476..3783)
                     /gene="brnQ"
                     /locus_tag="PWN45_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0623158.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4367..5257
                     /locus_tag="PWN45_001389"
     CDS             4367..5257
                     /locus_tag="PWN45_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL0623159.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5506..6555
                     /locus_tag="PWN45_001390"
     CDS             5506..6555
                     /locus_tag="PWN45_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL0623160.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6568..7245
                     /locus_tag="PWN45_001391"
     CDS             6568..7245
                     /locus_tag="PWN45_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623161.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7453..8484
                     /locus_tag="PWN45_001392"
     CDS             7453..8484
                     /locus_tag="PWN45_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0623162.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8827..9945
                     /locus_tag="PWN45_001393"
     CDS             8827..9945
                     /locus_tag="PWN45_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0623163.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9920..10843
                     /locus_tag="PWN45_001394"
     CDS             9920..10843
                     /locus_tag="PWN45_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDL0623164.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10854..12074
                     /locus_tag="PWN45_001395"
     CDS             10854..12074
                     /locus_tag="PWN45_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0623165.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12179..13711)
                     /locus_tag="PWN45_001396"
     CDS             complement(12179..13711)
                     /locus_tag="PWN45_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDL0623166.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13751..14632)
                     /locus_tag="PWN45_001397"
     CDS             complement(13751..14632)
                     /locus_tag="PWN45_001397"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL0623167.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14790..15650
                     /locus_tag="PWN45_001398"
     CDS             14790..15650
                     /locus_tag="PWN45_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0623168.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15928..16728)
                     /locus_tag="PWN45_001399"
     CDS             complement(15928..16728)
                     /locus_tag="PWN45_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0623169.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16868..17536
                     /locus_tag="PWN45_001400"
     CDS             16868..17536
                     /locus_tag="PWN45_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0623170.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17663..18976)
                     /locus_tag="PWN45_001401"
     CDS             complement(17663..18976)
                     /locus_tag="PWN45_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDL0623171.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6809 a         2930 c         3547 g         5951 t
ORIGIN      
        1 tagagataca tagcaatgta aaggtatcaa atagttattt cataatttat tatgatgaca
       61 taattttcag tagatagttg taaattaaca ctgaaataga tgtttatatt aagaagactt
      121 taatatcata attaatcaaa atcttgcgtc aaacatttaa acgaatttta agttgataac
      181 aattggcaaa acattgttca aacatcacaa tgataaagca tattatcagt attgtagtgt
      241 gtggaaaatg acagccatct aaggagaaaa atgatgaaaa gaatattggt agtattttta
      301 atgttagcaa ttatattggc aggttgttct aataaaggtg aaaagtatca aaaagatatt
      361 gataaagtgt acaaagaaca gaatcaaatg aataaaattg cctcgaaagt acaaaacact
      421 attaaaacag acattaaaca agaagacagt aatacacatg tttataaaga tggtaaagtc
      481 attgttattg gtattcaatt atataaagat cgtgaaaaaa tgtattattt cgcatatgaa
      541 ataaaagatg gtaaggcaga gattaataga gaaatagacc caattaagta tatgaaagac
      601 cataaagcag attatgaaga tgaaaatgta gaagtggaaa aagattaaca taggctttct
      661 ttaaaacttt taaccagttt caaaaagtaa atgagcttta gcgacaataa tttttaaaat
      721 aaacacgata taaatttaaa catttatata taagaacaca aacgcaagtt tgtaaataat
      781 aataataaag gagtaagaca atggaaaaat cgatcaaaat aatgacaata ataggaattg
      841 ttgttcaggg tttagcaacg gtatttagtt tactattgat ggttttagca gcatcaggtg
      901 taatgactac agatgtgtca acaacagtta atggtgaggt tgacccagtt gatgcagaaa
      961 cagcagcagc aattttcact gtattattct tatccctatt catatttgga atcatttcaa
     1021 ttattttagg tgcaatcggt atgtttaaag catctaaaaa caaaaaaatg agtggtatat
     1081 tgttgattat tggagctgta ataagtggta acataattac atttgcttta tggttaatca
     1141 gtggtattaa actacttact aataacaagc ctaaagatga aataagcgac ttatcataaa
     1201 catcgtatat tgaaatttca agatttctta agtaattaaa taaagtgctc tcttagaagt
     1261 agattttcag tcaataactg cttttaagag agcattttgt atttgataat gctcatagca
     1321 tcggataaaa atcattgacg aactttaaaa aaatacgtta gacaatgcac ttctaaatat
     1381 taaaggcatc gactaacgca ttgttcaaat atagttaatt atttttataa aattgatgat
     1441 gatcattcaa gtaagcatag aataaaccta taatgagtcc gcctccaata tagttaccga
     1501 taaaagccgc agcgatattc gaaatagctg gtatgaagtg caatgtatca acttgataaa
     1561 ttaaaccacc cataaataag caactgttgt aaacgacgtg ttcataaccc ataaaggcga
     1621 atatggtaac accgaacatc atgacaaaca tttttgcgag tacatcgtca atttgcatgg
     1681 caataactaa tgaaatattg ataaagaaat tggcgaagat cgctttcatt aatatactta
     1741 caaaaccagt agacaacgtt ttatgctcta taactgctga taactgattt aacatatctg
     1801 gcgtcattac atttgaaaaa cgcatgaaac taaataaaat agcagcacct aaaatatttc
     1861 ctgcaaagca taataaaaat attttcaata ctctagttgg tttaattact ttataataca
     1921 ggcctacagt aaagtacatg aagttactgg ttagtagttc ggagtttgta aataaaatga
     1981 gtactaacgc aaagctgaat gtaatggcac tggccatatt cacaatgcct ggcggtaaat
     2041 ctggttcgtg tgttgcttta actgataata cgaagaccgt aataatcccg ataataaatc
     2101 ctgccatcat cgcgcgtaat aaataacgtt ttaaataaac gctttgtaat atatcttttg
     2161 ttcttatcgt ttcgactacg ttatttaccc agtcgtcccc ataaaaaatc ttatcccatt
     2221 taatatgttt ctccttcaca atgacatcct cacttttcat ttgttaatca gagtatacta
     2281 tgaattgcct tatttgtgaa taatttcaca aaattaattt taaaaactaa tatttttgaa
     2341 taacagctag gattttttaa caaatcattt attttaaaat aatcaataga cagacggtat
     2401 cactgaaaag tcatgaattt catataaaaa agcctagtaa cgatgggtca atcattacta
     2461 ggcagtgagc atttattaag ttgtcgcttg tttcggacgg cgtataaata catcgattat
     2521 gaaaccgata atagcaaaga gcatgaatgg tacaagccaa gctaaatcga tatctgctaa
     2581 aggtaacatc ataaacgatt tcaaaataac accgtgtaat aagttgaaac tatttagtat
     2641 ttgtaaaatt gaaataatca atgtaataac agttgcgagt cgataggccc aactgaatct
     2701 gaatgtgcta aacatgttag caaatgatat gagtacaagt gcaatcgaca cgggatatat
     2761 taaagtcaat aatgggacag caatttttaa aatcatttct aaaccaagtg ttgtaaataa
     2821 gaaccctatg atagagaaaa taagtgcgaa tattttataa gaaaacttag gtacgtgttt
     2881 cttagtaaat gtggcgcaag cattgacgag tcctatacat gttgttaggc atgcaaggat
     2941 aaccgtcatt ccaaatacga ggttaccgaa cgaaccaaat aatcgtaatg agttgtacgt
     3001 caatatatct gtaccatctt taaagtttcc tggagctgtt gatgccccaa cgtatgcaag
     3061 tgcaaagtaa atcattccaa gtaatatggc tgcaataaga cctgaaaagc agacatattt
     3121 taaaattttc atgcgatctg tgaggccttt aaacttatag ccattgacaa tgactacgga
     3181 aaaagctaac gcagcaacaa gatccattgt aaaatagcct tccaaacttc ctgaaatgaa
     3241 aggatgtgtt atatatttat ccttaggtgc acttagtgca gattcagggt tgaaaatgac
     3301 agcaatactt aatagagcga ccattaatag taataacggt gttaataatt tacctaaatt
     3361 atcaacgatt ttcgatggat ttaaactaat ccagtaaacg atggcaaaaa agattgctgc
     3421 gaatataatt aaagtccatt ggttatgcac aggtaaaatg tgtcttgtac caatttcgta
     3481 cgcgacattt gcagcacgtg gaataccgta aaatgctccg atagacatgt aaatcacgac
     3541 agcaaaaata aacccgaacc atggatgtat acgattgcct acactttcaa caccttcatc
     3601 ataaaatgca acaacaataa cagtaataaa ggggagtaat atgcctgtaa gggcaaagcc
     3661 tagcatacca atccacatat tttgacccgc tgtatggcca agcatgggcg ggaatattaa
     3721 atttccggct ccaaaaaata gtgaaaataa catgaggccc gaaataataa cttgtttttt
     3781 caatgtaatt tccttcctaa aactagatat tcataataat ttaaaaaatc tgaaaaataa
     3841 aaacgttctt actttatctt taaatgacaa tactatgatt ctattatttt ataaaaaaga
     3901 ttgcaataat aacgtcttgc tattattgga taatgacaat taaagtgtcg tttttatgaa
     3961 ttaaaatagg tgaaagtaag ttggaaatac gttggagcgt tgtgattttt atgagaaatt
     4021 gtatgagtga ggactgctat ttataaaagg taaactatgg atgagtttat ggttgttcca
     4081 ataggtaaat aagagatagc acactgtaca cataatgata cgtggatgag tgaatatgtc
     4141 tgataggaag gcctagtcac tcaaatgaat ttaagtttat tacgtgataa atcacaaatc
     4201 tctctcatgt gataggtctc ccattaaatc atgcttatat gaaatgttca catatttgtt
     4261 agcttttcaa gaaataatat taaatatctt ttcatagtat agcaatatta aataaatgca
     4321 tttataattt taactgtatt ttaaatatta atcatgaggt gataagatga ataaaatttc
     4381 aaagtatatt gcaatagcat cattatcggt agcggttaca gtttcagcac cacaaacgac
     4441 aaattctaca gcgtttgcca aaagttctgc tgaagttcaa caaacgcaac aagcttctat
     4501 accagcatca caaaaggcga atcttggtaa tcaaaatatt atggcagtgg cttggtatca
     4561 aaattcagct gaagcaaaag cattatattt acaaggttat aacagtgcaa agacacagtt
     4621 agataaagag attaaaaaga ataaaggtaa acataagtta gctattgctt tggatttaga
     4681 tgaaacagtt ttagataatt ctccatatca aggctatgca tcaatacata ataaaccttt
     4741 cccagaaggt tggcatgaat gggtacaagc tgctaaagct aaacctgtct atggcgcaaa
     4801 agaattcttg aaatatgctg acaaaaaagg tgtcgatatc tactatattt ctgatagaga
     4861 taaagaaaaa gatttaaagg caacacaaaa gaacttaaaa caacaaggta tccctcaagc
     4921 taagaagagt catattttac taaaaggtaa agatgataag agtaaagaat cacgcagaca
     4981 aatggttcaa aaggatcata aacttgtcat gctatttgga gataatttat tagactttac
     5041 agatccaaaa gaagctacag ctgaatctcg tgaagcatta attgaaaaac ataaagacga
     5101 tttcggtaag aaatatatca ttttccctaa cccaatgtat ggtagttggg aagctacgat
     5161 ttacaacaat aactataaag caagtgacaa agcaaaagat aaattacgta aaaatgctat
     5221 taagcaattc gatcctaaaa caggcgaagt taaataatat atgaattgga cgtctacatg
     5281 tacttaaagg tatatgtagg cgtttttaaa ttgatcctta attttaaatg taccgtacaa
     5341 aaataataga caattaattg cattttccat aaatttgctt ttaatataaa aagttcgggt
     5401 ataatgaagg ataagtgtat gtataacatt acagagcgca acacgacgta cgtgcatcta
     5461 agaatataaa caatgtttaa atcatagtag tagggagaga aatgcatgtt tttagcttgg
     5521 aatgaaatac ggcgcaacaa attgaagttt ggactaatta ttggtgtgtt aacgatgatt
     5581 agttacttgc tatttttatt atctggattg gcgaatggtc ttatcaatat gaataaagaa
     5641 ggcattgata agtggcaagc agatgccatt gttctaaata aagatgccaa tcaaactgtg
     5701 caacaatctg tttttaacaa gaaagatatt gaaaataaat acaagaagca agctactttg
     5761 aagcaaacag gggaaattgt gtctaatggc catcaaaaag acaatgtttt agtgttcggt
     5821 gttgaaaagt catcattttt agttccgagt ttaatagaag ggcataaagc gactaaagat
     5881 aatgaagtgt tagctgatga aacgcttaaa aataaaggat ttaaaattgg tgacacatta
     5941 tcactatctc aatcagatga aaaattgcat atcgtaggtt ttacagaaag tgcaaaatat
     6001 aatgcgtcat cagtcatttt cacgaatgac gctaccattg ccaagatcaa tcctagattg
     6061 actggagata aaattaatgc agttgttgta cgtgatacaa attggaaaga caaaaaatta
     6121 aaccaagagc ttgaagcggt aagtattaat gactttattg aaaatttacc aggttataaa
     6181 ccacagaact taacattaaa ctttatgatt tcattcttat ttgtcatttc agctacagtt
     6241 ataggcattt tcctatatgt catgacatta caaaagacga gtttatttgg catattaaaa
     6301 gctcaaggat ttacgaatgg ctatttggcg aatgtggtaa tttcgcagac ggtcatatta
     6361 gcactatttg gtacggcatt tggcttactg ttaacaggcg ttacaggtgc atttttacct
     6421 gatgcagtac ctgtcaaatt cgatgtacta acattgctcg tatttgcaat tgtgttaatg
     6481 attgtctctg tattaggaag tttattctcc attttaacaa ttagaaaaat agatccgtta
     6541 aaggcgattg ggtaggaggt gtagcaaatg ttgaaatttg aaaatgtaac aaagtcattt
     6601 aaagatggga atcgtaacat tgaagcggtt aaagatacaa attttgagat aaataaaggt
     6661 gatattatag cattggttgg accttctggc tctggtaaaa gtacatttct aactatggca
     6721 ggtgctttac aaacaccgac atctgggcac attttaatca ataaccaaga tattacgaca
     6781 atgaagcaaa aagcattggc aaaagttaga atgtctgaaa taggttttat tttacaagct
     6841 acaaaccttg taccattttt aacggtaaag caacaattta cattattgaa aaagaaaaat
     6901 aagaatgtta tgtctaatga agactatcag caacttatgt cacaattagg tctaacttca
     6961 ttgcttaata agttaccttc agaaatttca ggtggtcaga aacaacgtgt ggcgatagcc
     7021 aaagcgttat atacgaatcc gtcgattatt ttagcggatg aacctaccgc ggcgttagat
     7081 actgaaaatg cgattgaagt cattaaaatt ctacgtgatc aagccaaaca aagaaagaaa
     7141 gcatgtatta ttgttacaca tgatgaacga cttaaagcat attgtgatcg ttcatatcat
     7201 atgaaagatg gcgtccttaa tcttgaaaat gaaacagtag aatagtttta ttaagccggt
     7261 acatcatgtg ccggtatttt tatgtttatg tattatttga ataaactttc acattcaatt
     7321 aataataatt attatcgaaa atcagaaata ttccgtgaaa tataatattt tttgtagtaa
     7381 aatggcctct aagtattcaa tatttaaata tggggattga atataaaatt atcgtaatgg
     7441 gggtcaatgg ttatggattt attgataggt actttatttt tatttttggt cttagtgatt
     7501 tttacattat ttacatataa agcgcctaat ggtatgcgtg ccatgggagc attagctaat
     7561 gcagcaatcg caacattttt agtggaagca tttaataaat atgttggtgg cgaagtattc
     7621 ggtattaaat ttttagaaga gctaggagac gctgcgggag gtctaggtgg tgtcgctgcc
     7681 gctggattaa cagcattagc tatcggtgtg tcaccagtat atgcattagt tatagcagcc
     7741 gcgtgcggtg gtatggattt attaccaggt ttctttgcgg gttatatgat tggatatgtg
     7801 atgaaatata cagagaaata tgtgccggat ggtgtcgact taattggatc gattgtcatc
     7861 ttagcgccat tagctcgtct tattgcagta ttattaacgc cagtagtgaa tagtacattg
     7921 attcgaattg gtgatattat ccaaagtagt acgaatacga atccaattat catgggtatc
     7981 attttaggtg gtattattac ggttgtcggc acagcgccat tgagttcaat ggcattgaca
     8041 gcattattag gtttaacggg tgtacctatg gctattggtg ccatggcagc atttagttcg
     8101 gcatttatga atgggacgct attccatcgc ttaaaattag gtgatcgtaa gtctacgatt
     8161 gcagtaagta ttgaaccttt atcacaagca gatattgtat cagccaatcc aattccaatc
     8221 tatattacaa atttctttgg tggtgcgatt gctggtttaa ttattgctat gtcaggttta
     8281 attaacgatg cgacaggtac agctacaccg attgcaggat ttttagttat gtttggattt
     8341 aatcatccga cgacaattgt gatttatggt gtagtaatgg cgattgtagg tgcgcttgca
     8401 ggttatcttg gttcaattgt atttaaaaaa tatccaattg ttactaagca agacatgatt
     8461 aatcgaggtg cagtagacgc atagcatcat catattgaat agtaaaaaca aataaaacat
     8521 agtaacgtga ttcagtcgat gtaacagtcg ataatgagtc acgttttttt atagaaaaat
     8581 acaagacata aaaatgtcat aatttattgt cgacaaatat catactgtat aaacatttat
     8641 cattttctca agtacctttt acacgatgga atgaacttac tttttacgaa attatgcgta
     8701 ttttataaac aaatatcatt gatataacgg taaatgtaag cgtttacaac agaaataaca
     8761 gcatgctacg atatttttgt aaattcactg attcaagtat tttaagtcaa tatgaggagg
     8821 gatgttatga gcgattctga gaaagaaatt ttaaaaagaa ttaaagataa tccgtttatt
     8881 tcacaacgtg aacttgctga ggcaattgga ttatctagac ccagcgtagc aaacattatt
     8941 tcaggattaa tacaaaagga atatgttatg ggaaaggcat atgttttaaa tgaagattat
     9001 cctattgttt gtattggcgc agcgaatgta gatcgtaagt tttatgtgca taaaaattta
     9061 gttgcagaaa catcaaatcc tgtaacgtca acacgctcta ttggtggcgt agcaagaaat
     9121 attgctgaga acttaggtag gcttggcgaa acggtcgctt ttttatctgc tagtggacaa
     9181 gatagtgaat gggaaatgat taaacgattg tccacaccat ttatgaattt ggatcatgtt
     9241 caacaatttg aaaatgcgag tacaggttca tatacagctt taattagtaa agaaggcgac
     9301 atgacatatg gcttagcaga tatggaagtg tttgactaca ttacgcctga atttttaatt
     9361 aagcgttcac acttattgaa aaaggctaag tgcattattg tagatttgaa tttaggcaaa
     9421 gaggcattaa acttcttatg tgcctatacc acgaaacatc aaatcaaatt agttatcacc
     9481 acggtttctt ccccaaaaat gaaaaatatg cctgattcat tacatgctat tgattggatt
     9541 atcacgaata aagatgaaac agaaacatac ttaaatttaa aaatagaatc tactgatgat
     9601 ttaaaaatag ctgctaaacg ctggaatgat ttaggtgtta aaaatgttat tgtgacaaat
     9661 ggcgtgaaag aactcattta tcgaagtggt gaggaagaaa tcattaagtc agttatgcca
     9721 tcaaatagtg tgaaagatgt tacaggtgca ggcgattcat tctgtgctgc agtagtgtat
     9781 agctggttaa atgggatgtc tactgaagat atattaattg ctggtatggt taacgcaaag
     9841 aaaacgatag aaacgaaata tacagttagg caaaacctag atcaacagca actttatcac
     9901 gatatggagg attataaaaa tggcaaattt acaaaagtat attgagtatt ctcgagaagt
     9961 tcagcaagca cgggagaaca atcaaccgat tgtagcatta gaatcaacaa ttatttcgca
    10021 tggtatgccg tacccacaaa atgttgaaat ggcaacaaca gtagagcaaa ttatcaggaa
    10081 taatggtgcc attccagcaa ccatagccat tatagatggc aaaattaaaa ttggtttaga
    10141 aagcgaagat ttagaaatac tggcaactag taaagacgtt gctaaagtat ctagaaggga
    10201 tttagcagaa gttattgcga tgaagtgtgt tggtgctact actgtagcga cgacgatgat
    10261 atgtgctgca atggctggta ttcaattttt tgttacagga ggtattgggg gcgtccataa
    10321 aggtgcagaa catacgatgg acatttcagc agacttagaa gaactgtcta aaacaaatgt
    10381 cactgttatc tgtgcaggtg ccaaatcaat tttagactta cctaagacga tggagtattt
    10441 agaaacaaaa ggcgttccag ttattggata tcaaacgaat gaattgccag cattcttcac
    10501 tcgcgaaagc ggtgttaagt taacaagttc ggttgaaacg ccagaacgac ttgctgacat
    10561 tcatttaaca aaacagcagt taaatcttga aggtggcatt gttgttgcta atccaattcc
    10621 atatgagcat gccttatcaa aagcatatat tgaggcaatc ataaatgaag ctgttgttga
    10681 agcggaaaat caaggtatta aaggtaagga cgccacaccg ttcttgttag ggaaaattgt
    10741 agaaaaaacg aatggtaaaa gtttagcagc aaatataaaa cttgttgaaa acaatgcggc
    10801 gttgggtgct aaaattgctg tcgctgttaa taaattattg taggtgatga tacatgaata
    10861 ttttattcgc tatcacaggg atagcatttg cactatttgt tgcgttttta ttcagttttg
    10921 atcgtaaaaa aatagacttc aaaaagacgt taataatgat atttattcaa gtgttgatcg
    10981 tgttatttat gatgaacaca acgattggtt tgacaatttt aactgcacta ggttcatttt
    11041 ttgaagggct aataaatatt agtaaagcag gcataaattt tgtttttgga gatatacaaa
    11101 ataaaaatgg ctttacgttc tttttaaacg tattactgcc attagttttt atttctgtat
    11161 taataggcat ctttaattat attaaggtat taccatttat tatcaaatat gtaggtatcg
    11221 ctattaataa aataactaga atggggcgct tagaaagtta ttttgctatt tcaacagcaa
    11281 tgtttgggca accagaagta tatttaacaa taaaagatat tattccaaga ttatctagag
    11341 cgaaattata tacaattgcg acgtctggta tgagtgctgt tagtatggca atgctaggtt
    11401 catatatgca gatgattgaa cccaagttcg tagttacagc agtaatgtta aatattttta
    11461 gtgcgcttat catcgccagt gtaatcaatc cctataaatc tgatgatact gatgttgaaa
    11521 ttgataactt aacgaaatcc acagaaacta aaacattgaa tggaaaaaca ggaaaaccta
    11581 agaaagttgc ctttttccaa atgattggtg atagtgcgat ggatgggttt aaaatcgctg
    11641 ttgtagtagc cgtaatgttg ttagcattta tttcattaat ggaagcaatt aatatcatgt
    11701 ttggtagtgt tggtttgaac tttaaacagc ttattggcta tgtgtttgca ccaatcgcat
    11761 tcttaatggg gattccatgg agcgaagctg ttccagctgg ctctttaatg gcgactaaat
    11821 taattacaaa tgagtttgta gcaatgcttg attttaaaaa tgtcctgggt gatgtatcag
    11881 ctcgaacaca aggtatcatt tcagtttact tagtaagctt cgctaatttt ggtacggttg
    11941 gtatcatcgt aggttcaatt aaaggcatta gtgataaaca aggagaaaaa gttgcatcct
    12001 ttgcaatgag gttgctactt ggttcaactc tagcttcaat catttcagga tcaatcattg
    12061 gcttagtatt gtaaatgaat cgaagtacct aaattaaatt catggcaaag ctaaaccccg
    12121 tcaccaagtt ggcgcaacag cgcatcataa cttagtgacg gggttttatc ataacaatct
    12181 actttttcgt agccgttttt gaaatgtatg ttgatggttt atctttttca aaaattgtta
    12241 atcccgttat atctttttta tgttttgaag ggacaatgaa gctaagtata taagcaaaga
    12301 caaaagcaac tgtaaatgaa atggtagata catagaaagg tgagttacct ttgccaacac
    12361 cattatagac ataagcaaag atgataccca atattaatcc acaaataaca ccgaatgtat
    12421 tcgtacgttt agtgaaaata ccaactgcaa atacaccagc caatggaacg ccgaataatc
    12481 cagtcacaaa caagaataaa tcccataagt catttgaatt agaagcaatt aagtatagtg
    12541 acattccaaa accgaaaata cctgcaatga taataatgaa acgtgcaaag ttaacttcgt
    12601 gtcgctcgct accttttccg aagaagcgtt gcttaatgtc gattgaaata caagcagata
    12661 tagaatttaa actagatgaa atggtagact gtgcagcggc gaaaatggct gcaataagta
    12721 atcctgctac aaatggtggc atctcagtca aaatgaaata tggcactaca gatgatgtat
    12781 tgaagccttt tggtaaaaca gcttcatgtg tataaaatga atacagcatt gtacccatac
    12841 cataaaataa gggtgctgaa attaaagcta ggataccatt tgtccataac gatttatttg
    12901 tttcttttaa actatcagaa gcttgataac gctgcacgac gtcttgactc gctgtgtatt
    12961 gatacaagtt gttgaaaata tttcctagga aaataattgg aatggcagct gccgcagtat
    13021 ttagtttcca attgtctgca ctaattaatt ttttgtgctc aatcgcatct gcaaagacag
    13081 tgccgaaacc gcctttaatg ttcacaacac ctagaataat aataactaaa gcgccgccta
    13141 ataaaatgac gccttgaatg aaatcactcc aaaccacacc ttcgaaacca cctaaaaatg
    13201 tatataaaat acatagtaaa ccaacgagtg atgcaacgat ataagggttc atgtctgata
    13261 cagatgtgat tgctaatgtt ggtaagtaga taacaattgc aacacgccct aaatggtaaa
    13321 cgacaaataa taatgagcca atgacacgta tgctagggcc aaatctagct tctaaatatt
    13381 catatgcaga tgttaccttt aactttttaa agaaagggac atagaaataa ataagtaatg
    13441 gaataattgc gacgatagca atgttaccag cgatatatga ccaatctgtt aaaaatgctt
    13501 tctctggtgt cgacataaat gtaatcgcac ttaacgtagt agcataaatt gaaaagccaa
    13561 ctacccaaga tggcaagcga ccacttgcgg taaagaaact attggtactt tggctcgcgc
    13621 gcttggtaaa ataaacgcca atgaacaaca tagctagtag ataaatgata acggcaaccc
    13681 agtttagtgt gccaaatcca acttctttca tgggcaacat cccctttaca atgtattgat
    13741 tctttgatgt ctataaatcg tattttgcaa tgagttgatc taatgtttgt cgatgtgctt
    13801 cgttaaaagg tttgaaaggt cttttcggta atcctgcatc aatgccacga tgacgtaata
    13861 tttctttcaa tgttggataa atccccattg ataacactgt ttcgataatg tcgtttgaat
    13921 catgttgcag ttggtaagct tcttgaattt gaccttgtcg tgctaagtcg aagatttttc
    13981 ttgcacggcg accattaacg ttatatgtag aaccaattgc accatctacg ccagaaatcg
    14041 tagcttgaac taacatttca tcaaagccag ataagattaa tttgtctggg aatgcttttc
    14101 taatacgttc gagtaggaag aagtttggcg ctgtatattt aacaccaaca attttttcat
    14161 gattaaatag ctcgctgaat tgttcaatag aaatattcac acctgttaaa tctggtattg
    14221 cataaataat catattgttc tgagttgctt cgataatatc gaaatagtaa tctctaattt
    14281 cttcaaaagt aaatggatag tagaatggtg ttacggcaga aagtgcatca taaccgagtt
    14341 ctgtggcata ttttccaagt tcaatggctt catttaaatc taacgaacct acttgagcaa
    14401 tcaatttcac tttatcccca actgcctctt tggcaacctt gaaaacttgc ttcttctgct
    14461 ctgtatttaa taaaaagttt tcgcctgagc taccatttac ataaagaccg tctaattctt
    14521 cagtttcaat ggcattttga gcaatttgtt taagtccttg ttcatttact tgaccatttt
    14581 catcaaaagg aacgagtaac gctgcatata aaccttttaa atctttgttc attatgaagt
    14641 ccctccaaaa atcatttgat aatatagttt acagctataa ttgtaaacgc tatcataaaa
    14701 tgtaacaata tctttttgaa aattgtagtc atatttatgt ataattaatg aaaatgtttt
    14761 tcaaaatcaa tagaaatgga gtgagtaagg tgtattacat cgcaatcgat attggaggca
    14821 ctcaaattaa atcggcagtt attgataagc aattgaatat gtttgactat caacaaatat
    14881 caacgccgga caacaaaagt gagcttatta ctgacaaagt atatgagatt gtaacaggat
    14941 atatgaagca atatcagttg atccaacctg tcataggtat ttcatcagca ggcgttgttg
    15001 atgaacaaaa aggcgaaatt gtatacgcag ggccaaccat tccgaattat aaaggtacta
    15061 attttaagcg attattaaaa tcactgtctc cttatgtcaa agtaaaaaat gatgtaaacg
    15121 ctgcattact aggcgaattg aaattacatc aatatcaagc agaacggatc ttttgtatga
    15181 cgcttggtac aggcattggg ggtgcgtaca agaataatca aggtcatatt gataatggtg
    15241 agcttcataa ggcaaatgaa gttgggtatt tattgtatcg tccaactgaa aatacaacgt
    15301 ttgagcaacg tgctgcaacg agtgcattga aaaagcgcat gattgccgga ggatttacga
    15361 gaagcacaca tgtgccagta ttgtttgaag cagctgaaga aggtgatgat attgcaaaac
    15421 aaatattgaa tgagtgggca gaagatgtag cagaagggat tgcccaaata caggtcatgt
    15481 atgatccagg gcttatatta attgggggcg gtatatctga acaaggagat aatctcatta
    15541 aatatatcga gccgaaagtt gcacactatt taccaaaaga ctatgtttat gcaccaatac
    15601 aaacgactaa gagtaaaaat gatgcagcat tatatggctg tttgcaatga tagttgaaag
    15661 aaggagtcat tctaaaatag aatttgaaac cgttacgaga gatgagagct gttgttagtt
    15721 ccacacatca cactctatct aggaccaatc taaactatat caaccaacag tgtgccacgg
    15781 gcaaattaaa ttgaagaagc tgagatatta aaattttaga aaatgtaaaa aaatatttgg
    15841 tattgaaatt aaaaaagcac ctagcaactc gttgggacaa tcacgatgat tgtctacagt
    15901 tgcaggtgga tttgaatata ctactagtta tttgttgtct aggataatag atttagtatg
    15961 ttgataagtt tgactcagat tcgtattttc taataaatga taactcacga tatcgattaa
    16021 aaagagtgtc gcaatttgtg tgttgataaa ttgatggtcg gtattacgcg attgatccgt
    16081 tgttaaaagt actaaatctg cacaatctgt aagtttacta ccttcaaaat ttgtgatggc
    16141 aacgacatat gcaccatgag atttggcgac ttccgctgca gaaattaatt ccgaagtatt
    16201 accactattt gacatagcaa taaacatatc cgaatgagat agtagggatg ccgatatttt
    16261 cattaaatgt gaatcggtag taacattacc ttttagcccc atacgaatca tacgataata
    16321 aaattcagtc gctgataaac cagagctacc tagtccagca aagagtatat gtcgacttga
    16381 ttgaagtttg tcgataaagg tttggataat gtcgttatca ataaattcac cagtttgttg
    16441 aatgatttgt tgatgatatt tatgaattct ttgaataatt gggctatttt caataactgt
    16501 ctctgtcatt tcttgttgaa tattaaattt taaatcttgg aaattctcat aatccagctt
    16561 atgactaaag cgtgtcatcg ttgctggtga tgtaccaatc gcatgggcta aggagttaat
    16621 cgttgaaaag gcatcgctat aaccattttg tcttatataa ttgacgatgc gtttatcagt
    16681 ttttgtaaat aaatgttgat aacgttgaac acgattctca aatttcattg tgtcacccct
    16741 tcatcttaat gattactatt atatatgaaa aatattttca agatagtaaa aagcattgat
    16801 aaaaattatc ttaatgatat attgtaaatg actttacgtg aaaaaacgac ttatggagtg
    16861 aggaataatg ttaccacatg gattaatagt atcttgtcag gcactaccag atgaaccatt
    16921 gcattcatct tttattatgt cgaaaatggc attagctgcg tatgaaggtg gtgctgttgg
    16981 tattcgcgca aatactaagg aagacatttt agcaattaaa gaaacggtag atttaccagt
    17041 tattggcatt gtgaaacgtg actatgatca ctcagatgtt ttcattactg caacgtcaaa
    17101 agaagttgat gaactgatag aaagccaatg tgaagtcatt gcattggatg caacgttaca
    17161 gcaacgtccg aaagaaacgt tagacgaatt agtatcatat attagaacac atgcaccgaa
    17221 cgttgaaatc atggctgata tcgcgaccgt tgaagaagct aaaaatgccg cacgacttgg
    17281 ctttgattat attggcacga cgttacatgg ctatactagt tatacgcaag gacaattact
    17341 ttatcaaaat gacttccaat ttttaaaaga tgtactacaa agtgttgatg caaaagttat
    17401 tgcggaaggt aatgtcatta caccggatat gtataaacgt gtgatggact taggcgttca
    17461 ttgttcagtc gttggtggtg cgataacacg accaaaagaa attacgaaac gttttgttca
    17521 aattatggaa gattaaatga taacgataaa aaaacgagat gaccatcatt aattaaaggc
    17581 acctaattat cttaggtggc tgaatgaatg taatgggttc atctcgtttt gtttgtttat
    17641 gatagtgatt ttattttcaa ctttatccaa aaataagtaa agcgacgggg atggtgatta
    17701 atagcgacaa cgccacgcgt aaaaaccaaa tgatgatgag tttccagaca ggtattttaa
    17761 tttcagttgc tagtatacat ggcactaatg ctgagaaaaa gataatggct gatacgctta
    17821 ctacaccgac gacaaattta gtactcattg cagctttagt tactaacaaa gatggtagaa
    17881 acatctctac aatagaaatc gctgacgctt ttgctagtaa agcctgatca gcaattggga
    17941 aaatataaat aaatggatag aagatatagc caagccaatc aatgaatggt gtatagttcg
    18001 ctacaatcag tcctaaaaaa ccaatcgata atatagaagg taaaatacca acagtcattt
    18061 ctaaaccgtc tttcaaattg tcccaaacgt tcttcacgag agatggtgtt aatgcatttt
    18121 gtttcatcgc ctctgcatat gcagttttca gtctgcttcc ttcaatagca acttcttgtt
    18181 ctccttcttg tccgttataa tattctgttg attcattgct gattggcggt agccatgcag
    18241 taattgcagt cacgacaaat gtgatgacta aagttatcca aaagtataaa ttccaatgcg
    18301 gcattaatcc taaagtttta gcaacgataa tcataaaagt tgctgaaact gttgaaaagc
    18361 cagtcgcaat aatcgtggct tctcgtttgt tgtacatccc ttgcttatag acacgattag
    18421 taatcaataa tcctaaggaa taactgccga caaacgaagc cactgcatcg acagcggatt
    18481 ttcctggtgt tttaaaaata ggtctcataa taggctccat ataaacaccg acaaattcta
    18541 ataagccata gcccactaat aaagaaagcg caattgcacc tactggaatt aagatactta
    18601 atggcatcat taatttttca aacaaaaacg gaccatagtt agctttaaat agtattgatg
    18661 gaccgatttt aaatacatac attataccga tcattgcacc tgcaacttta aataatgtaa
    18721 tgaccaagtt tgtgattgaa gtcataaaag tacgtctcac tattggtaac gctgtaccaa
    18781 ttaaaatcat aatcagtgca acatagggca taagtggacc tatgattgag cgaatggcta
    18841 gatgaacatg atcgacgaaa atagtgttgt taccattaat cgtaaaagga ataaagaaac
    18901 atagtatgcc cactaaacta tagacaaaaa aacgccatgc acttggttgt tgtgcattag
    18961 aatgatattg attcattaaa gcaacccctt tgtttaaatg aatacacaaa actgtatgat
    19021 gcatcttccc cttaatgaga tgaatcatta ttttaattta gaaaaatctg aaaacttact
    19081 ataattgtat agtttgaatt attttcatac caatacaaat taactaatta tatatagatt
    19141 gaaactatat tacttaataa aatatttatc ttaaatgttg ttgtgttgat tcaacaccac
    19201 aactaaaagt gtttataaat tatttggaaa tacacat
//
LOCUS       DANJFZ010000046        19139 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.046, whole genome shotgun sequence.
ACCESSION   DANJFZ010000046 DANJFZ010000000
VERSION     DANJFZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.046"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(78..269)
                     /locus_tag="PWN45_001402"
     CDS             complement(78..269)
                     /locus_tag="PWN45_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0623172.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="PWN45_001403"
     CDS             430..759
                     /locus_tag="PWN45_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0623173.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="PWN45_001404"
     CDS             772..1233
                     /locus_tag="PWN45_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDL0623174.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="PWN45_001405"
     CDS             1251..1685
                     /locus_tag="PWN45_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623175.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="PWN45_001406"
     CDS             1714..2109
                     /locus_tag="PWN45_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623176.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="PWN45_001407"
     CDS             2199..2345
                     /locus_tag="PWN45_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623177.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..2956)
                     /locus_tag="PWN45_001408"
     CDS             complement(2342..2956)
                     /locus_tag="PWN45_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL0623178.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            3082..4287
                     /locus_tag="PWN45_001409"
     CDS             3082..4287
                     /locus_tag="PWN45_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0623179.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(4330..6356)
                     /locus_tag="PWN45_001410"
                     /pseudo
     CDS             complement(4330..6356)
                     /locus_tag="PWN45_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6349..7278)
                     /locus_tag="PWN45_001411"
     CDS             complement(6349..7278)
                     /locus_tag="PWN45_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDL0623180.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            complement(7523..9274)
                     /gene="srrB"
                     /locus_tag="PWN45_001412"
     CDS             complement(7523..9274)
                     /gene="srrB"
                     /locus_tag="PWN45_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDL0623181.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(9255..9980)
                     /gene="srrA"
                     /locus_tag="PWN45_001413"
     CDS             complement(9255..9980)
                     /gene="srrA"
                     /locus_tag="PWN45_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDL0623182.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(10113..10850)
                     /locus_tag="PWN45_001414"
     CDS             complement(10113..10850)
                     /locus_tag="PWN45_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0623183.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(10843..11385)
                     /gene="scpB"
                     /locus_tag="PWN45_001415"
     CDS             complement(10843..11385)
                     /gene="scpB"
                     /locus_tag="PWN45_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL0623184.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(11378..12109)
                     /locus_tag="PWN45_001416"
     CDS             complement(11378..12109)
                     /locus_tag="PWN45_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL0623185.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            12201..12707
                     /locus_tag="PWN45_001417"
     CDS             12201..12707
                     /locus_tag="PWN45_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDL0623186.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(12785..13672)
                     /gene="xerD"
                     /locus_tag="PWN45_001418"
     CDS             complement(12785..13672)
                     /gene="xerD"
                     /locus_tag="PWN45_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL0623187.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(13721..14170)
                     /locus_tag="PWN45_001419"
     CDS             complement(13721..14170)
                     /locus_tag="PWN45_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0623188.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(14275..14817)
                     /locus_tag="PWN45_001420"
     CDS             complement(14275..14817)
                     /locus_tag="PWN45_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0623189.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            14899..15807
                     /locus_tag="PWN45_001421"
     CDS             14899..15807
                     /locus_tag="PWN45_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0623190.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(16023..16271)
                     /locus_tag="PWN45_001422"
     CDS             complement(16023..16271)
                     /locus_tag="PWN45_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623191.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(16287..17042)
                     /locus_tag="PWN45_001423"
     CDS             complement(16287..17042)
                     /locus_tag="PWN45_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0623192.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            17184..17999
                     /gene="proC"
                     /locus_tag="PWN45_001424"
     CDS             17184..17999
                     /gene="proC"
                     /locus_tag="PWN45_001424"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL0623193.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(18104..19024)
                     /gene="rnz"
                     /locus_tag="PWN45_001425"
     CDS             complement(18104..19024)
                     /gene="rnz"
                     /locus_tag="PWN45_001425"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL0623194.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
BASE COUNT         6407 a         3347 c         2670 g         6715 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataata aatttaacaa ttaaaggatt aaatacaaac actgttaaaa
     2701 caaaaataaa tagaaacaaa atatttgctt ttagacctgt aagcaactga attaaattca
     2761 aattttttaa atcaacatta ttaaaaatta taaaactata aaaccatatc aaacatgttt
     2821 caatagaaaa aatcaataat acaggagtat tgataacctt gtttttttca ctaactaaac
     2881 ctatcattgt tagatattta tatggtatgt aacctaaaac tcctgtaaga agaagcgccc
     2941 ctagaaattg agtcatctta tcacctactt tttattttat tataacacat ttagtaccta
     3001 gtactaaatt acgggtagcc cgactaccct tattattttt taatatttta tagaacatac
     3061 gttcttgcag gaggtataaa catgtggatt gaaaaattta aaaacaaaaa taacgaaact
     3121 aaatacagat actacgagaa gtataaagat ccatacacag ataaatggaa gcgcgtaagt
     3181 gttgtgttga acaagaatac aaaacaatct caaaaagaag caatgtttcg tttagaagaa
     3241 aaaataaaag aaaaactgaa caacaagtcg tcaagcgaat taaaaacttt gacttttcac
     3301 gcgctattag atgaatggct tgaatatcat ataaaaacat caggttcaaa gttgactact
     3361 cttaataata taaaaataag aattagaaac attaaacgat acagctctga gaacttgctt
     3421 ttaaacaaac tagatacaaa atatatgcag atatttatta ataaattatc agatatctat
     3481 tctcaaaatc aagtaacccg tcaactcgga gatatgaaag gagctattaa atatgcagtt
     3541 aaattttaca attatccaaa tgaatatttg ttaactaatg tcaaaattcc taaaagaaga
     3601 aaaacaatag aggatatcga aaaagatgaa tctaaaatgt acaactattt agaaatgaac
     3661 caagtcctac agatacgtga tcatatacta aatgataata agttacacaa gcgaaatcgc
     3721 attttaattg ccagcatctt agaagtacag gctttaactg gtatgcgcat aggagaacta
     3781 caagcactgc aggaaaaaga tatagattta ttaaacaaaa ctatcaatat aacaggtaca
     3841 attcaccgca ttaaatacga ggaaggattc ggatacaaag acactacaaa gactataagt
     3901 tcaaaaagaa gtatcagcat caattctaga accgtagaaa tttttaaaaa gataatactg
     3961 gaaaacaaaa tgttgaaaag atggaattcg agctatgttg acagagggtt catattcaca
     4021 acaaaaaaag ggaatccttt atgtaataat caaatcgccg gtgtgcttaa gaaaactaca
     4081 aaagctttaa atatgaataa gaaagttacc acgcacacat ttagacatac acacataact
     4141 ttattagtag aaatgaatgt ttctttaaaa gcaattatga aaagggtagg acatgtagat
     4201 gaaaaaacaa ccattcgcat atatactcat gtaactgaaa aaatggatag agaactaact
     4261 caaaaactcg aaaacattcc aagttagctt aaatccgccc tttttttgcc cttatatttt
     4321 ttacaagctt tataaaacgc ttgagaacac tggcgttaaa gcttttcttg aaataaacat
     4381 atcatcataa tgtgatggtt caaatatcat ttgtacaatc aaaggcttca tgttcttaac
     4441 aatatcatct aaatggttat ctaaaattgg tgacactgct tttaaatcat taagaaaagg
     4501 ctcccatttg cctaaagtat tatctaattc ttctaattta gttttaatat aattacaagt
     4561 tacattagga atcagggaca aaaattcttt cttttttaca tttaacattt caattgcatg
     4621 tcttaaattc ttacgtattt tgggaattgt attaatcaaa tattttatta catcgacaat
     4681 tttcgatgca tattcatcat atataccttg aacatagtct gctatttttt taataccatc
     4741 atcgatatgg tcttttaata ttttcatttt tcttcctaaa taattagaag gtataactag
     4801 accctgtacc atattttcgc cgctacaatt aatttgaaaa tttccatcta aaattgttgc
     4861 atcttgttgt ttcataatac ttctaatatc tgcaatttgc ctaccataaa tatcattttg
     4921 attttttatt tggtctatat tctgtttcac tactttcaaa tgtttcatca tttcttcaga
     4981 tactccatct ctgaagtcgt gatctatatt tttgaaaatt tctaaaattt cattatctat
     5041 actatcatac actttttcta taaaagattt tataccttta aacaactcat taattctttc
     5101 ttttaatgca tccaatgcaa aatcaggtaa taagtgttta acagcactaa tactttctat
     5161 tgtttcatct gcaacttctt caagtgagtt tattttacta attaaagttc tttccatttc
     5221 ttctaactga aataagttaa tcttatcctt aaatccttct gataattgtt tctttctatc
     5281 tgcaaaattt ttattttcat tttctgagat gttaaaactt tcatttaaaa agattacgca
     5341 ttctgctaac ataccacttg tttcaccagt aatcagttta ctcaacgcat caagattttc
     5401 taaattaagt ttaattaaag ttcctcttcc agaacgtgca atcgaatctc ctgtccaaac
     5461 atttattgga attcgcccat ccatatctaa tgttatgtta atagtctttt ttactttttt
     5521 ccatttttaa tttctgtatc ttttaccgac tttattttga ttagtggtac agtatcgtat
     5581 gtgttatctt ttctatttaa cttcctttta taacctacat ggctgtctat taaagtatct
     5641 aacctgggca caccatcatt aatgttaacg cgttttcctg gcatatcttt gatgaatgga
     5701 tcttgtaacc atgttaataa atcgttggta ctattaaaac taatcatatt atcaaagcgt
     5761 ggtctagcaa atttctgcca agcagcataa ggaatcattg ctgagtcagt agcaacaact
     5821 ttttcattcg gatgtttcgc tccttgatat tttgctcctg caccgccttc cgaattaccg
     5881 ccatccgcca caatggtttt gtttttgaag ttacttgatt ccattctata tttttttaaa
     5941 aaatcactat ttgataatct atctgcatct tctaacttta ttttatattg atttgataat
     6001 tgatctgttt gctttaaata atctgtgctt tcattatcat tattcattaa tttagcattt
     6061 tggagccaat catctccaaa ccccgatgat tttaatggat tatttggatt aattgcctca
     6121 ttagatgttc cttgataaat tattgtctgt tgaccagttg gtttactttt atcatctaat
     6181 aactcatata ttttaatatc agcagcacca ttcaaattac tattcttatc atcattataa
     6241 ctgtctactt gtttaaacct ttttccatta actttaaatt ctttttttat atcaatattt
     6301 tgataaaccc agtaactact taattctgtt aaatccctat cattaatttt attcatcttt
     6361 actgtgcacc ccatattcta ttggacgatt atcatcataa aaggctttct tagtattaat
     6421 agtgggtttc cctatttgta ttgtgattgt agatttatct ggtttatttt ttaaattata
     6481 taatttatca cttagttcga ttacatcatc tactgtattg tctttagtaa agtttttctt
     6541 agtagaaaaa agtgtagcaa cagcatctgt atttgcagcg taattcactt cttttcttgc
     6601 tcgttccatt ccttctttaa attctttatc attttttcga atcagtggtt cataatactt
     6661 tcgatattct tttaaacttc tagaagagta tgtaatataa aaatattcat ttttatatcc
     6721 gacattttgt gtcttgttaa ttgcctcttt tgtaaagcct gtatattgat atttcttttc
     6781 attttctttg aagaatttat ataagttatc atacttttct ttttgcgctc gatattcaaa
     6841 gccactcagc actgtaccca ccatcatact catatcatca ccattgtcat tactgcgcat
     6901 tgatcctttt tgatggatgg catctttgta caaaggtaga cttgcattaa atacaatgcc
     6961 atgatcttca caatgcacat aaacttctac accatcatct ttacctacaa catttgtagc
     7021 tttaactttt agtccaaagt tatctttaaa gaattgttca cctacttttt caaattcttt
     7081 acgatgcttc ttcgcaaatt caatcgcatt tttttctgca ggcggttgaa agccttggcc
     7141 tacatatttt gaagcttcca tttcttctgg tacagatttt gtttctgttt ttgtgtcttt
     7201 ttttgattca ttttccatcg tggaacatcc ccctaaaatt aatgtcgtag ctaaaactga
     7261 tccaatgaat tttttcatat gacacctcga ataatttaat taatacaata tatctatatt
     7321 ttaccctatt tttataatca aataaataac tttatcaaac tttatatttt tgtataaatt
     7381 agtaatatat ttaatagttg atattcgcaa aaaccctaag ttctaatcca catacaaaat
     7441 tccccacatc aatcaaaaaa cgcatgctat aaattagagt ctctaatcaa taacatgcgt
     7501 tctgaaataa ctatattcaa ttttattctg gttttggtag tttaataata aatgttgtgc
     7561 ctttccctaa ttcgctttta acatctatgg aaccaccatg ctcttcgata atcattttac
     7621 aaatgaacaa acctaaaccg gtaccttgtt tacctcgcgt tctcgctgca tcaactttat
     7681 aaaaacgatc aaatacttgt tgtaaatgtt ctggtgcaat gcctgtacct gtatctttaa
     7741 tgtataaaat atcttcgctt tcattttcat cacaagtaat tgcaatttca tctccaggtt
     7801 tcgtataacg tgatgcatta tcaattaagt tcgttagtac ttggtccatg cgatccatat
     7861 cataactcca aacacgcttc ttacaataat taaaagtcat atttagacct aaatcatcag
     7921 cttgttggcg atacttaatt ttcatcttat ctagtaacgc tgcaataggc tgaacttctt
     7981 tatttacgga taacccttca gcatccatgc gtgcgacatt taacaattca ttaactaaac
     8041 gatttaaacg tttcgattca tcaaggacaa tggcaagcga ttcttttatt tcatccggtt
     8101 ctgtaacaat accatctaca attgattcag tataaccttg aagtaatgat atcggtgtac
     8161 gtaattcatg tgatacatta gcaatgaaat ctttcttcat ttgatctaga ttgtgctcat
     8221 tagtcatatc acgaactgtc acaacaacac cacttttacc tccctgttca atcttgtcga
     8281 tatagcttgt ggtcacaaca aagaatcgtg cattcatttc taaatcgcgc atttcagttt
     8341 gttttgattt aaaagtatct tctatttgtc ttaataagaa agctttagca tcttcatcaa
     8401 tattgtccat aatatcattc gccatcttat tagataagat aatttgtcga ctctcattaa
     8461 tacctaggac accttctacc atagagttaa ttaagctgtc tctaatattt ttagatgtgg
     8521 ataatgcgtc gacatgctct tcgatttctg tactcatctg attaaatgcc tgcgataatt
     8581 gaccaatttc atctttcgtt gtgacagaag gtttataaga gtaatcccct tcagatacac
     8641 gtgtagcttg gtctcttaaa cgtcttaaag gttttgtaat tcttgacgat aagaaaaacg
     8701 caaagactgt tgtaattgtt aagaaaataa cagccgtaat tatggtgata atcgtaatag
     8761 cattatttgt atcttcgatt gatttcaagt ctttatatat aaagactcca ctatatttgc
     8821 tatgactatt cttctgtgct tttgttggat agcctaacaa aatatatgtt tgagatgagc
     8881 ccttttcttt aatcgttaca tttcgagtaa cagatttacc tttatcaaac acatcgtcaa
     8941 aatggtcgtt gttgactact tcattcaaca tttgcttttt aatattagaa agtgaagccg
     9001 ttgattgacg atgtttatta tttataatca tcaacccacc aggattttca attaatgttt
     9061 gactatattt tattgcttct tctttattat gtgattgttc gaccagtgaa cttatacgtc
     9121 tagcatcttc tcttatggca ttttcggttt cttgtgtgaa atagtattgc ataaaggtaa
     9181 ttaaagcaat acttaataaa attaaaactg tcgtcactat taaaataata gttaaccaca
     9241 gtttaattac gacactattt agccggctca tcattagatt taacctcaaa tttataccca
     9301 acgccccaga ctgtttgaat catatgcgca gcttcactag acacacgatt taacttttct
     9361 ctaagtcgtt taacatgagt atcaactgtt cttaaatcac catagaattc ataatgccaa
     9421 acttctttta ataattgttc acggtcaaat actttatttg gtgttttagc taaatatatt
     9481 aataattcgt actctttagg agtcaaatta acttcttgat tatcagcaag tacgcgatgt
     9541 gcatcattat ctatttctaa atgtttaaat tcaatcacat cacgtgcgtg aggttcgctt
     9601 tgttctacag ttgtagattg cgttcttctt agaagtgctt taactcttaa gactacttct
     9661 cttggtgaaa atggtttgac gatataatca tctgcaccag attcaaaacc ttcaacacgg
     9721 tttgtttctt cacctttagc agtcaacata ataatcggtg tttgtttatg ttcacgcaat
     9781 ttagttgcca cctggatacc atccatttca ggcaacatta aatctagtag tatgcaagca
     9841 taattattct ccattgcaag ttcataagcc tcttggccat tacttgcttc atggatttca
     9901 aaagattctc tttctaaata cattttaagt aatcttctga ttctatcctc atcatctacg
     9961 ataagtattt cgttcgacat acaggtcata cctcccacac atgcttttct ttacaaaagt
    10021 attatatcac aggaaagcgc atacagttat taaaaaccta aaattttgtg aaaaaattct
    10081 caaactttgt gataaataca tattttattt aattatttcc catgttctgc taaatgacgc
    10141 atcactttta cttcatgagg cgtcaataca cgtccttcac cagcattcaa accgacaaca
    10201 tttaaaggcc catattcaat acgagacagt ttcgtcactt gatgaccaaa atgttcgaac
    10261 attcttctga cttggcgatt acgaccttct gtaattgtaa tttcaaccaa tgttgtgttt
    10321 ttatctttat cttgtttctt aactttcact tcagccggtt gcgtcatacc atcttctaat
    10381 tcaatacctt tttctagcgc tttcacttct tctctcatta aataaccttt taatttcgca
    10441 acatattttt tcttaatttg atatcttgga tgtgtcatta aattagtaaa ttcaccatca
    10501 tttgtgagta ataacaatcc agaagtatca tagtctaaac gaccaacagg ataaatacgt
    10561 gcttctatat ctttaaagta atctgtaacc actgtacgtc ctctatcatc agatacactt
    10621 gttatcactt gagttggttt atggaataaa atgtaaattt tgtcttctag ttctatttta
    10681 ataccttcaa cttcaatcgt atctgatggc ttcacttttg ttcctaattc agtgacagtc
    10741 gtaccattca ctttcacttt tccttcagaa attaaagttt ctgccttacg tcttgaagta
    10801 taaccgctat tagctatacg tttttgtaat cgttctaatt ctttagtcat tattttctcc
    10861 tttttgattg actagattac tgaaaaaagc atccatttct tcatcgtctt cttcagttgt
    10921 cggcaaatct tctatattgg aaataccaaa tacatttaaa aataaatcag tagtaattaa
    10981 ctgttggcta cgctgttcat taaccacttt agcctcaact agtcctttgg caatcaatgt
    11041 cttaactgca ccatctgaat tgatactacg aattaattca atatcacttc ttgataatgg
    11101 ctggttataa gcaataattg atagtacttc cattgctgct tgtgataatt tcatttgtga
    11161 cttttgttca attaattgtt caatatacgt tgccgcttct tttttagtcg ttaaaacata
    11221 cgtcattcca aatcgttgta tcattaatcc atgtgatgaa taattttcaa ttaattcaac
    11281 gagttggtct ttcgacatat ctaatatttc taatagttgt ttttcatcta aaccttcatc
    11341 gccagctgta aataaaagcg actctaatat accatgatta tccaaaatgg tagttcactc
    11401 ctctaataat gttaatatct tcaaaattac gttgctgctc aatattaatt attcctgctt
    11461 ttgacatctc taaaatagct aaaaagtgag tgactacttg ttcaattggc tcagaaaacg
    11521 taaacagact aaagaagtta aaatgatctt tatctttcaa tctcgatgtc acttgttctg
    11581 tagcttgttg aatggtaaat gtctcttttc ggatttcaac agatttaggt gtatttaact
    11641 caactctatt tttaactctt tgataagcta caattaattc agttaaatca atcgtatgat
    11701 ttggatccca agattcatct gtttccaaat gagataaatc tgtcggtctt tttgtaaaat
    11761 aaaaatctct ttcttctttc atgtcattta aaatagcagt atattcttta taattttgat
    11821 attctattaa acgcccaact aaatcttccc gtgggtcatc atcaacatcc atatctgatg
    11881 ttgattgtgg taatagcatc ttacttttaa tcattaagag ttctgacgct aatactaggt
    11941 attcacttgc aatattaatt tcaagctgtt tcattgcatg aacgtactgc atatactgct
    12001 ctgttaatgc ttgcatagga atatcataaa tatctatttc aaatttttgg ataagatgca
    12061 gcaataaatc taatggtcca ttgaaagcat ctaatttaac ttcatacata atatctacct
    12121 cgtattgcgt catatgtgca taattctcta aaatattata gcatgaaaat aacttacttt
    12181 taaattgggg tgataacaaa atgcaacagg cactgataaa tttctattat caatttcata
    12241 ctaagcaaca ttatttccta tgtcatgaca ttttagaaga tgcatggaaa gctgaaaata
    12301 actacagtaa gcaagatgca gttgttagtt taatcttgtt tgcaactgct tgttaccatt
    12361 atcgccgcaa taatttaaaa ggcgcctata agtcttttaa taaatctaaa gaaataatac
    12421 aaaatgctaa agatagagat gcgttatacc ttaatttaaa tgattatcag ttgttaattg
    12481 agcaacagat tgcaaaattg aatgcagcaa agcctttttc atctgttata ttaccaataa
    12541 ctccagtctt tgaacgcatc atcaaggcca actatccaga ctatgattat aatcaggaaa
    12601 cagcaacaga tccttttatt gtagaccatc acatgcgccg tgatcgttca gaagtgattg
    12661 cagcaaaaga agaagcgatt caattaagaa aacatagacg taattaatca accaatttaa
    12721 aaaagccaca atagcacaga catttttagt catgtgtatt gtgacttttg agttattgtt
    12781 cactttatgc tctaggatga aattggttat acatttttct aatttgagat ttcgaaacat
    12841 gtgtatagag ttgggtagta gatatgtcag agtgacctaa catctcttgc actgctctta
    12901 aatctgcgcc attttccaat aaatgtgtcg caaaagagtg gcgtaacgta tgtggcgtta
    12961 acgtcttttt aatgtttgcc tttacaccat tttgtttaat cattttccat attgcttgtc
    13021 gtgataaagg tttaccatgc atatttaaaa ataagacttc agtaacagtc ttttttaaaa
    13081 gttgcggtct aatcgtttca atataagtag ttaagtactc aatgactgcg tcgcctaatg
    13141 gtacaattct ttctttatcg cctttaccaa atacgcgtac aaatcccatt attaagttca
    13201 cgttttctaa ctctaaatgt atcaattcag atacacgcat tcccgttgcg tacagaagtt
    13261 ctaacatcgt acgatcacga tatccattaa ttttatttaa atctggcgtt tctaataaag
    13321 ccaacacttc gtcaacattt aaaacgtcag gcaatttttt gtcatatttt ggtgaatcta
    13381 ataataccgt tggatctttc gccgcatatt tttctcttat agcaaattga tgaaaactac
    13441 ggattgttga aataaatcgc gcaatagatt tagcagattg cccttggtct attaaatgcc
    13501 ccaaacactc ttgaattaat tgtcgatcta taaaatcaat atgcgagata tgatgttcag
    13561 tcatataatc ttgatacttt ttcaaatcac gtctataagc accaattgtg ttagaactta
    13621 gtcctttttc aatttgtata aaacgcaaat attcttcaat aattgtttcc ataaccacac
    13681 ctctaaaaaa agcgaaggtt aatttaacct acgcaattta ctatccttta cctttagctt
    13741 ggcacgtttc acacacacca tggaaagtta aacgatgatc taaaatttta aaattgaact
    13801 cattttcaac tcgattttca acttctggta acaaatcttc atcgatttca tctacacgac
    13861 cacattccat acatactaaa tgatggtgga aatgttttgc gccttctttt cttaaatcaa
    13921 aacgagcgac gccatcacca aagttaattt tgtcgacaac ttttagttca gctaacaact
    13981 ctaacgttct gtatactgtc gccaagccaa tttcaggcgc tttatctttt actttcagat
    14041 atacgtcttc agcacttaga tgatcttttt cattttcaat tagaactcta acagtagctt
    14101 cgcgttgtgg cgttagctta tatgatgatt gttgtaattg ttgcttaacg cgatttaatc
    14161 gttcttccaa cgatgtccac tcccctacta ataattaaaa tcattacaaa ttatttcaaa
    14221 cttcacaatt aaaactaaca gttttctcaa taaaatgcaa gcttttctca tttgttattt
    14281 agaatgatta taatttaata ataggtgttg caatgcaatg atagtcttcg catcttctat
    14341 ttctttattc attagcatcg atttaacatt ctcaatcgga actttaatga cttcgacaaa
    14401 ttcatcttca tctaagtgaa ctgtaccttc ttctaaatta tccgtaaaat atattgataa
    14461 ttgttcatcg caaaaacctg gtgaaccata catatctaca acatgtgtta actctttggc
    14521 aatatatcct gtttcttctt ctaattcacg ttttgccgct tcgactctat cttcatcatc
    14581 ttctaattta ccagctggaa tttctagcaa tggtttttct actggtttac gatactgttt
    14641 cactaatacg acttcttttt taggtgtcac tgcacaaaca gcaactgcac cattatgata
    14701 aactaattct cttgttgacg tttcaccgtt tggtaatgtc actgtatgaa tttctacatc
    14761 tacaattttg ccattataaa taactgttcg atcaattgtt ttttcattta aatccattat
    14821 aatcacgttc ctttaaattc atattatata ttgatacact atgcttgtta gctaaagtgt
    14881 atcgaaagga gaacagacat gcaaaaaaat atattaaaaa gtggtatttc attatctgaa
    14941 ttaggtctag gttgcatgag tttaggtaca gatttaaaaa aagccgaaca aattatagat
    15001 tgtgctgttg aaaatggtat cacttatttt gatacagcag atatgtacga taaaggtata
    15061 aatgaatcag ttgttggtaa ggcacttctt aaatatcaac aacgcgatga tatttttatt
    15121 ggtacaaaag taggcaatcg tttaacaaaa gatggcagta caacatggga tccgagtaaa
    15181 tcctatatta aagaggcagt taaaggttca ctaaagcgtt taggtatcga tcatatcgat
    15241 ttatatcaac ttcatggcgg aaccattgat gacccattag acgaaacaat aagcgcattt
    15301 gatgaattga aacaagaagg aattatacgt gcttacggta tttcttctat tcgcccaaat
    15361 gtaattgatt attatttaaa acatagtcaa atcgaaacga taatgtctca attcaatttg
    15421 attgataatc gtccagaatc attattagat gcaattcaca acaatgatgt taaagtattg
    15481 gcaagaggac ctgtgtctaa aggattatta acttcaaaca gtgttaatgt gctcgacaat
    15541 aaatttaaag atggtatttt tgattattct catgatgaat tgggtgaaac aatagcctct
    15601 attaaagaaa ttgaaagtaa tttatctgca ttgacattta gttatttaac atcacatgac
    15661 gtgcttggtt ccatcattgt aggtgcaagt agcgtcgacc aattaaaaga aaatattgaa
    15721 aactatcata ctaaagttag tttagatcag attaaaacag caagagctcg tgtaaaggat
    15781 ttggaatata ccaatcattt agtgtagaag tcattttcag taataaaaac agcagcatga
    15841 ggcgtttcat tataaaaatg ccttactgct gttgtttatg tacaattcgc tataatttat
    15901 gattatgatt actcacttat gatagaaatt aaagcgttgt cctcacgcat cagtatttag
    15961 taatttcgcc ttgcggcatt gccttaagca aacttctgcc acttcatctc ttaataattt
    16021 tattaaaaca tctttctata tttcacttcg catgttgatt catcattatt agttattatt
    16081 tgtacaccca gcacatttcc ttgcaacaca agtagtttga atttttcaca agtataatat
    16141 aatgtaccgt ctgaaatttg gtctacagaa atatcgccta aaatatccag cactgtaaat
    16201 tcttcaaata ctgatagttg ttccgcatat cgtacacaaa gtcttaccac actctccgat
    16261 tgacagttca ttgccatccc acctatttat gctttatttt taaataattt agggaaacat
    16321 cgttcaaaaa atctaggcgc aatttgatac attttcaacg catgatgcat ccatttaggc
    16381 cgattaattt ccaattgttt tgttttaatg ccataaatga tatcttctgc aagctgatta
    16441 gcatcaagca taatttcccc catcttttta gcatacttca ttgatgggtc ggctttttga
    16501 tgaaaaggtg tatcaatcgg gccaacatta actgtcatga tatgtaagtt tggtgactct
    16561 agtcttaaag cattcattaa tgcataaaac cctgctttcg atgccccata atgtgcagca
    16621 tttgcttgtg tggaaaatgc agcttgactt gaaataccta caatatgtgc gttagatgtt
    16681 aaatatggtc tcaacacagt atataaaaca ttaaaactaa ttaaattaag ctgatacgtt
    16741 tcaatcattt ctgaaaaact atggtctgaa atagatttga aataacctaa acctgcacta
    16801 taaatgaatc catcgaatga tgtattgtct tcaaattgca gtgcctgtat cgacttcaaa
    16861 tcatttaagt cacaaggaat aacatttata gttttcccca attcctgttc aaagattcta
    16921 gttgctttat caacatcacg caccaacaac gttacatgca ctttattttc tagtaacttt
    16981 cggacaatcg ataaacctaa accactcgta ccaccagtca ctataaaatg ttgtcctttc
    17041 atcaattaac cttccttttc aattatatag aatgcaattt atcaacttta cataattgag
    17101 acaagttgat tatctttcct aatatatata caataataag aaaatataac atacaaatca
    17161 aaaactaaag ggatgtgacg ttaatgaaac tcgtatttta tggagctggt aatatggcac
    17221 aagctatatt tacaggaatt attaactcaa gcaacttaga tgccaatgat atatatttaa
    17281 caaataaatc taatgaacaa gctttaaaag cattcgctga aaaactaggt gttaactata
    17341 gttatgatga tgcgacatta ttaaaagatg cagattatgt atttttaggt accaaaccac
    17401 atgattttga tgctctagca acacgcatca aaccacatat tacaaaagac aattgcttca
    17461 tttcaattat ggcaggtatt ccgattgatt atattaaaca acaattagaa tgccaaaatc
    17521 cagttgctag aattatgcca aacacaaatg cgcaagttgg acactctgtt actggcatta
    17581 gtttttcaaa caactttgac cctaaatcta aagatgaaat taacgattta gttaaagcat
    17641 ttggttctgt aattgaagta tcagaagatc atttacatca agtaacagct atcaccggaa
    17701 gcggcccagc atttttatat catgtattcg agcaatatgt taaagctggt acgaaacttg
    17761 gtctagaaaa agaacaagtt gaagaatcta tacgcaacct tattataggt acaagtaaga
    17821 tgattgaacg ttcagatttg agcatggctc aattaagaaa aaatattacc tctaaaggtg
    17881 gtacgacaca agctggcctt gatacattgt cacaatatga tttagtatct attttcgaag
    17941 attgtctaaa cgctgccgtc gaccgtagta ttgaactttc taatatagaa gaccaataaa
    18001 aacaaacccg ccaacacatg tatgcatcat cgcaagcact gtgtttgacg ggttattttt
    18061 ataatttatt gttatttggc aagcattgtt tattactttg tcattagatt ttaaaactat
    18121 caaaatcttt tacaaaatta aaattaggtg tatcttcatt ttgtatcaat gtttgataaa
    18181 tttcatttat atcttctgta ttatagcgat tgctcaaatg tgtaatcaac gtacgtttaa
    18241 cattggcttc ttttatcaat gcaaatacgt cttcaatatg gctatgatga taattgttgg
    18301 ctaaatgctt ttcaccatct atataggtcg cttcatgtac catcacatca gcatctctag
    18361 aaatcacacg ttcattagaa catggttttg tatcaccaaa aattgctaca actggaccct
    18421 gtttggactc acctctaaaa tcttttgatt gataaacttg accattatgt tcaaatgtat
    18481 catgagattt tacttcttga tatttaggac ctggttcaag accaatgttt tttaacgctt
    18541 caacattgat tgtacctgta gtttcaggtg ccattactct atatccatat gatggaatac
    18601 catgattaag taaatgcgcc tctacagtaa aaccatcatg atgatatgtc agatgatcat
    18661 cgatttcaat atatgtaatt ggatagttta aatgtgactc tgataaattc atagacattt
    18721 ccacatatgc tttaattcct tttggtccaa ccaatgtaag cggcttctgt tcaccgccct
    18781 gaaaagaacg actagaaagt aatcctggca aaccaaaaat atgatcgcca tgcatatgag
    18841 taataaatat atgtgtcact tttcctaatt taattgcatg atgtaaaatt tggtgctgtg
    18901 taccttcacc aacgtcgaaa agccatatgg aattggaata tggttctaaa tttaaggcga
    18961 ttgcttgtgt atttctctct tttgtaggca aacctgcact cgttccaaaa aatgtaactt
    19021 ccatatatgc ccctcctttt cttcaattca ttttatcata aaatttgtat caatttagtt
    19081 ttaatttata cttgaatcaa aatctgttaa aaattcgtct ttgctagtta atttttcta
//
LOCUS       DANJFZ010000047        18798 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.047, whole genome shotgun sequence.
ACCESSION   DANJFZ010000047 DANJFZ010000000
VERSION     DANJFZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.047"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            582..956
                     /locus_tag="PWN45_001426"
     CDS             582..956
                     /locus_tag="PWN45_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDL0623195.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(1207..1995)
                     /locus_tag="PWN45_001427"
     CDS             complement(1207..1995)
                     /locus_tag="PWN45_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623196.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(2164..2880)
                     /locus_tag="PWN45_001428"
     CDS             complement(2164..2880)
                     /locus_tag="PWN45_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623197.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(3042..3548)
                     /locus_tag="PWN45_001429"
     CDS             complement(3042..3548)
                     /locus_tag="PWN45_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623198.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(3684..4484)
                     /locus_tag="PWN45_001430"
     CDS             complement(3684..4484)
                     /locus_tag="PWN45_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623199.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(4468..5187)
                     /locus_tag="PWN45_001431"
     CDS             complement(4468..5187)
                     /locus_tag="PWN45_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0623200.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(5242..6192)
                     /locus_tag="PWN45_001432"
     CDS             complement(5242..6192)
                     /locus_tag="PWN45_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0623201.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(6340..7227)
                     /locus_tag="PWN45_001433"
     CDS             complement(6340..7227)
                     /locus_tag="PWN45_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0623202.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(7606..8241)
                     /locus_tag="PWN45_001434"
     CDS             complement(7606..8241)
                     /locus_tag="PWN45_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDL0623203.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(8629..10290)
                     /locus_tag="PWN45_001435"
     CDS             complement(8629..10290)
                     /locus_tag="PWN45_001435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL0623204.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(10287..10703)
                     /locus_tag="PWN45_001436"
     CDS             complement(10287..10703)
                     /locus_tag="PWN45_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL0623205.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(10796..10903)
                     /locus_tag="PWN45_001437"
     CDS             complement(10796..10903)
                     /locus_tag="PWN45_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623206.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(10978..11988)
                     /gene="adhP"
                     /locus_tag="PWN45_001438"
     CDS             complement(10978..11988)
                     /gene="adhP"
                     /locus_tag="PWN45_001438"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDL0623207.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(12487..13008)
                     /locus_tag="PWN45_001439"
     CDS             complement(12487..13008)
                     /locus_tag="PWN45_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDL0623208.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(13036..14331)
                     /locus_tag="PWN45_001440"
     CDS             complement(13036..14331)
                     /locus_tag="PWN45_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0623209.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(14371..14847)
                     /locus_tag="PWN45_001441"
     CDS             complement(14371..14847)
                     /locus_tag="PWN45_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623210.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(15016..15552)
                     /locus_tag="PWN45_001442"
     CDS             complement(15016..15552)
                     /locus_tag="PWN45_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDL0623211.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(15763..16701)
                     /locus_tag="PWN45_001443"
     CDS             complement(15763..16701)
                     /locus_tag="PWN45_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0623212.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(16971..17597)
                     /locus_tag="PWN45_001444"
     CDS             complement(16971..17597)
                     /locus_tag="PWN45_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDL0623213.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
     gene            complement(17621..17866)
                     /locus_tag="PWN45_001445"
     CDS             complement(17621..17866)
                     /locus_tag="PWN45_001445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623214.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(17832..18467)
                     /locus_tag="PWN45_001446"
     CDS             complement(17832..18467)
                     /locus_tag="PWN45_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDL0623215.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(18504..>18798)
                     /locus_tag="PWN45_001447"
     CDS             complement(18504..>18798)
                     /locus_tag="PWN45_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0623216.1"
                     /translation="YLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKSNGIEKF"
BASE COUNT         6194 a         3386 c         2395 g         6823 t
ORIGIN      
        1 tatcaatcgg aataacgtat agacacttta acgtgctata gattggtttt aatcactaaa
       61 ttaatgtgtt tttcttatca ttaaaactgc actgagaatt actaaattaa aaaaattata
      121 aaaatttttc atttttagtg ataaaattct gaaaaatggg tataaatagt agaagaagtt
      181 aacttggaag agttaagcta taacaaagaa tctctttaga cacacattga atatcgaaac
      241 atttaattgc gctaaatcgt ttcattaaat aaattacctt gtattgtcga ttaaattaag
      301 gtaaattata aaaaatgctg atatttttga ctaaaccaaa tgctaaccca gaaatacaat
      361 cactgtgtct aatgaataat ttgttttata aacacttttt tgtttacttc tcatttttaa
      421 ttagttataa ttaactaaat aatagagcat taaatatatt taataaaact tatttaatgc
      481 aaaattatga ctaacatatc tataataaat aaagattaga tatcaatata ttatcgggca
      541 aatgtatcga gcaagatgca tcaaataggg aggttttaaa catggcaatt acaaaaatca
      601 atgattgctt tgagttgtta tcaatggtca cttatgctga caaattaaaa agtttaatta
      661 aaaaggaatt ttcaattagc tttgaagaat tcgctgtatt gacatacatc agcgaaaaca
      721 aagagaaaga atactatctt aaagatatta ttaatcattt aaactacaaa caaccacaag
      781 ttgttaaagc agttaaaatt ttatctcaag aagattactt cgataaaaaa cgtaatgagc
      841 atgatgaaag aactgtatta attcttgtta atgcacaaca acgtaaaaaa atcgaatcat
      901 tattgagtcg agtaaataaa cgaatcactg aagcaaacaa cgaaattgaa ctataatttt
      961 gtttagcgca atttggtgaa gtttgataga tgatacattc tattaaactt ccttttttta
     1021 tgctcttttt acctaattgt taagaggttt tgcactaatg gcacttaata taaggaagat
     1081 agccgcataa cgagcagtat atatcaaata catttcaaaa aaagctgatt tctatcaaat
     1141 aatgaatagt aatcagcttt ttcacattgc ctaaggactt tatgccctag gtttatatat
     1201 cacatattat gatacgcttt ttaaaaattt taaataataa ttcataaatg cttctggttc
     1261 ttcaatatgc ggtgcatgtc ctgaatgttc gaagattttt ttcgtaacat ttaaaaaatg
     1321 cgcttccaat ttaaaatgat tcttatcttt aatcaaagga tcatgttccc cattaattaa
     1381 caaagtaggt acacatgtac gtttttttaa atacataggt tcatctttat gcaaaattaa
     1441 tgaacgaatt gccaccgcac tatcatcctc agacttaatg ctatatattc tttgtttttg
     1501 ataccatttc atcgtcttct cttgatttct aaacaattta gaaaataaaa gaatatacgc
     1561 ttcagactta ttaaaacccg ctatatcatc ttgatattgt attaacaatt gagtaaagct
     1621 gtgtattaag tcatctttct tcgatgcaat ggtagttaat gattttacac gataagcata
     1681 tttatctgta aataacttag caactacccc acctaaatca tgtcctagaa tatgtgcctc
     1741 atgaatattt aacttctcca ttagaatttt taaatcctca acgtgatcgt ttaaatcgta
     1801 tgattcactt ttagaagact tgccatgacc tcttaaatcg taagtaagta ctttatatga
     1861 tgatgctagt tgatgttgca aatcttcaaa tccagctaaa ttaccatcca atccatgaag
     1921 taatattata ggatcccctt taccttcaat ttgataagtt aattggattt gaggattaat
     1981 tgtgacttta ttcattgtga cacctccctc aataaactat atacccaacc ttcattctat
     2041 atacgcttcc attttataaa caattttttt aaattattta taaattacca aaagttattc
     2101 caacaataaa aaaactattc atctcaatta atacaattaa tcaagtgaat agttttatca
     2161 tatttataat cttgtacgtg aagataatct aattgtgaaa tatataacta gaactactaa
     2221 taatatccat ataattaatg aaaaaatctg ggctgattgt ggattatgaa tcattgcatc
     2281 gacggttttt tgacaaaata ataacccgat tcccgaaaac tcaagtcgct tataaaatat
     2341 gccggcaatt actaaaataa taccaatgaa aaagaaaaat tgatgtccaa aatttgtaat
     2401 tggtataacg ccccaagtaa tattaaatgc ttgtaatata attaataaca aggatatgat
     2461 agcgataatt gtacccataa tcatttctat tttattacta cgcaagtaat tcttttgaga
     2521 cacccttagg tatacaacaa tagtcattgg aaaaatcact aaacaataaa atagactcat
     2581 tccagtaata gcttgaaact gaacatactg tttatcatat aaataggata tgagaatgaa
     2641 gtttataata aagaaaataa ctggattcat ttgtaaagca aacaaattcc tttgaatcat
     2701 taatatgatt tgtgctggag attttctttt atattcaata taatcttgat ctttcttttc
     2761 agaatttttt atatcagtaa ttactttctc tttaatatca tcaatcaaat gcggtgataa
     2821 acctttatga ttaaagtaat cttctatatt ttctagtaaa attttatcgt taatacgcat
     2881 tggtagtact cctttaaaat gaaacatctt tatttattgt attggtttaa tttccccttc
     2941 gcaaatcata aaaattaata ttcactgtat tatgccatgt aataaaataa ccgacaagta
     3001 ttgcgattta acttgtcggt taagcacgat taatttaatt attatttttc caaatcaata
     3061 cgatataatt ttatgttttt atcttttggt gctgaggcaa attgataagt cgttttgcct
     3121 ttttcaatat atccaaaatt accagcaact ttgctatatt ccgtatgtgt tactgctttg
     3181 ccaacataat ttttaacagc ttgatatgtt ggaccattag catcattata catcattgaa
     3241 atatgagtta ctttaccatt gtttttctta ccatcagtag ttactaataa catacctttt
     3301 ttagtatgga attcgtaata atgttcagtt ccatcttcat tgtaagaata catcggattt
     3361 ttgtacttag ttaaaacatc tttaatagat tcgccaattt tcacattttc taatgactga
     3421 tcacccttaa ttaattgttt aacagtatca atagagttgc cacttgctgc ttcggcacta
     3481 gtgttcacta agcctactcc cattacaaca gaacatgcaa ttatacttgc cgttagtagc
     3541 tttttcataa gcactctctc cttttattta tatcgtcttg tgctcacaac cattatacaa
     3601 caatcatttc taaaacaaca gtcatattac aattatatta caaataataa ttactacttt
     3661 tatatttcac ttatcactaa aaattaaaca tgcttttcat cgtgatgttt gttgataaat
     3721 gcagctgtat cttcgacgaa tctttcttgc tcttcaacaa atgggaatgc acttgattct
     3781 tgatatactt caaagtccgc attttggatt aaatcagcta cttctttagc ttctaatctt
     3841 gttgttcttt caccgaattc tcctgcaata atttttgtcg gtactacgac atttctataa
     3901 gtttgagaaa tatcagcgtt tttgaatacc tctttaactg cttgaatttc ttctttagtt
     3961 gatatttcat ttgtatctac gacatgtttg aggaatcgat tcatttttct cggacgataa
     4021 tacttacgct tatttaaaaa tttatcttgt ttttcaggat cccagtttcg aataatatgg
     4081 gcatattttc taaataaacg ttcttccggt aattcacctt caatagatgt tggatttacc
     4141 aacgtaagtg atgatgtaaa ttcaggataa cgtactgaaa tatccgcacc aatgattcca
     4201 cccatttcat ggcatacaaa tgcaacttct tcaatatata aatatttaag taattcaaca
     4261 atgtcatcag aaaaatcttt tatttcaatg tgacgaggtt tatcagaata gccatgtcca
     4321 cgtaaatcaa ttaacacaac ttgaaatgat tttgctaatt ctgctgctaa tttattaaac
     4381 acagaataat tatcaagtac agtatgaatc aatacgatag gatagccttc acctaatgta
     4441 ctgtaatgta tcgatgttcc atcttttcta gtaaatagat ccataatttt ctcctttatt
     4501 atcatttttt attggtaaaa tggttaataa ttcttccact gttgtaattg taaaatcaac
     4561 ttcttcttcg agtggttcaa tttcagcatc ttcttgttta taccatacac taaccatgcc
     4621 catagctcgt gctggagcta catcatttaa cgcatcatcg ccaacataca taatttgctc
     4681 aggtaatacc cctagttgat caatcatatc ttcaaaaatt ttaggatgtg gtttacgaaa
     4741 accaactgtt tctgatgttg ataaataatt aataacatgc atcaaaccaa gtgaatgtaa
     4801 tcgaaattgt ttaatcttag atttaccatt tgcgataaca ccaatttgaa agcctttttg
     4861 cgatagcttt tctagtgtat acaaagtatc gtaataagga aatacataac gataaaaatg
     4921 catttcaaaa tcattaaata agtctttcca agttaaccta tctacatgga aacgtttaat
     4981 gatttcttta tataaatcag gtttatcatt atcttcatcg tcatctagct caataaatgc
     5041 ttttttaaaa tctgccagtt gaacatgaat taagtagtca tgaaatcgtt catattgctc
     5101 ttcgataaat ttctctcgag atttaactct gtccaacaac gtaccttcta aatcaaatac
     5161 aactgcttta atttgattca aatccatcgc ttatgccctc tttatgttta tagttatttt
     5221 tattttagca aatgtcgtgt gctagtgtaa ccgtttcgac tgagatatta gcatgtaaaa
     5281 taacactggt ataccaatta atgcaatgat aatacttgcc ggtatttgta ctggtgacaa
     5341 tatatggcta ccaataaaat ctgccataac cattatttgt cctccagtta caatatttaa
     5401 agccaattgt cttcctaatg attgtttcca ctgccatttt ctaatgagtt gcggtatcac
     5461 cataccaatg aatccaatga tgccaacata tgcgacattt atcgccgtta tcatagaagc
     5521 tatacataac gcgatatatg taatcaattg aacatttaag cctagtgact gactttttag
     5581 ttctcctaat tgcaataact ttagttgatt aagaatgata aatataccaa acaatgcaat
     5641 gataaatgtg attgctatta tagatacatt tgagtattct gcatcaccaa aaccaccaaa
     5701 cagataattg gcaattgtat ttaatttgcg aggttttaat aaaatcaaaa aatatagaag
     5761 tgaattgaat aacgcaccaa tcattaaacc acttaatatt aagattctaa ctggatagcc
     5821 ttggctcaat accgacgtaa tgactaatac agttattaat gttatcaaac taaatgttat
     5881 tgaaaataca ggaatccata acgttgttaa acctaaaaat aatgctaatc ctgaaccaaa
     5941 tgtagcgccg cttgccaatc ctaatgtaaa gctatctgcc aatgcattat ttaaaactgt
     6001 ttgaaatata aggcctgcaa cagttaatat catacctgtc aacaatgctt ctaataatct
     6061 cggtaatcta acattgatta aaatagactg gttaaataca tcattgatat cgcccaactg
     6121 ccaaaataga tatatgctag ttgttataat caatatgact atccagctca ataatacttt
     6181 attaaatgtc atagcattct cttttctttc atcgataatt acattcatta tagcaatcac
     6241 atactcgcgt tgctatcatt cactaaatat attttaaaaa ccccttatct caaacatgta
     6301 ttttttacat gtagcaataa ggggcataat tagaatggtt tattttctat aaattgcatc
     6361 tcttaattct tttaatcctt catcaatacg tggacctggt cttgatactt catcaccatt
     6421 tacaacttca atacgtgtat tcttgacagc attaatttta ttgaatccac ctcttttttt
     6481 gatgatatcc atataatctg atcttgtctt agcttccgtc gaaattaata tatctgggtt
     6541 ctttttaata atactttcct tcgttacagg gttccaacca ttaatgtcac tatacacatt
     6601 ttgggcttct aatttttcta acatatcatt aaaaaatgta tgcttccctg ctgtatatat
     6661 ttcaggcttt gatgaaacct caataaatac ttttgatttt ttatgatgag caggaattga
     6721 atctatgact ttatcgatat tatctttagt ttcctcaaca agttgttcag cctgcttatc
     6781 atgatgcgtt aatttcccaa tttgcttaaa tgtgttgtaa gtttcatcaa ttgattgtgc
     6841 atctttaaca tacactactt tgatgccttg tttctctaat gatgacaata ctttattagc
     6901 agttgccttt tgcgactcat gcgcaagaat tagatctggc tttgccttta aaagttcctc
     6961 tttatttaga ttcaaagcat caaattgttt cttacccttt ttcacatctt ttggataatc
     7021 atcaaccgtt gaaacaccaa ctatgtattt acctaatcct aattcatata aaatttcagt
     7081 attactaggc attaacgaaa caattctatg gtacggcgat ttagacgagc cattatcatt
     7141 gctaccttgt ttatcattat ctttcatacc acagccactc aggacaagca taaaaatcaa
     7201 aataaaagca attaacgatt tcttcacact atcacatctc ctttttaatc ccacttttcg
     7261 ctttagcgat tagagggatt tatgcagttg tgcggtagca tccaactgcg ttcctcttca
     7321 tctttagcga ttagaggctt ttatgcagtt gtgccttagc atccaactgc gttcctcttc
     7381 acctttagtg attagaggct tttatgcagt tgctgcacaa acaactgtat tcctttactc
     7441 cccttgagct ttagcgatta ggggctctta tgcagttgct gcacaaacaa acaactgttt
     7501 ttttctttat atgcttctat ttattatata agtccacata ctaaaagaaa atagtaaatt
     7561 attttttata tttcatataa actcatctat ctaacttcac agcgtttact ttttaaagta
     7621 aggttctaaa aaatcataat tctttatatc tttgtctcta aagtaatgtt taccaaatac
     7681 atctaacaga gcatgaaatt cattagcatc ttgatttgta aaatgatttg gtaatgtgac
     7741 tacttttttt aattgatcat aatttttagt gttttcatat cctaatttat catatatttt
     7801 tcttgtatag ctatctggaa taaattcaat acgtccgaat atataaacaa gtaagacatc
     7861 tgctgtttca cttccaatac ctttcaaaga taataattct tttcttaatc cacctttata
     7921 tcgctcatta atctcttgat aattgaaatg atgtcgtgct aaccatgtta ataatgtttt
     7981 aatcgtcagt gatttacttt tataaaagcc acttgaatgt atcaatgatt gtaacgtttc
     8041 aataggtagt tctaatatat gatttggatt aaaatgcgta tgttctttaa tctgattcaa
     8101 tgcaatttct gcatttcgcc atctagtatt ttgaactaaa attgcaccta acatcatttc
     8161 aatgtcatta tcagcaggcc accaaaattg tggtccgaga tgttcatata aaactttata
     8221 taattcatca gttcctaaca tttacaccct ccattttaga gggagtggga cagaaatgat
     8281 attatcgtaa aatttatttc gtcgtcccac cccaacttgc attgtctgta gaaattggga
     8341 atccaatttc tctttgttgg ggccccgccg gcaaggttga ctagaattga aaaaagcttg
     8401 ttacaagcga attttcgttc agtcaactac tgccattata actttgtaga gcatagaaca
     8461 ttgatttatg tctcaggctc tcttatgcag ttgtgcagag catccaactg tgttcctctt
     8521 aaccttattc acatactaag cattccccaa tatgtcacat acctaaaaaa acactccaac
     8581 acaaagtatc tttcgagaaa ctctacgttg gagtgtatat aaatgttctt acattgattc
     8641 aggtgcgctt acaccgacca ttgccaatgc atttttcaat gtaattctga ccgcttcaat
     8701 catagcaaca tgtgcttttg ttttttcaat atcatctgtt aacacttttt cagcattata
     8761 gaatttatgg aaatgagaag ctaaatcttg aatataatta gtaattctat gtgccgatct
     8821 atgctcagca gcactttcaa ttgtaggttc gaaatcagct acttttttca acaattcaat
     8881 cgctttttca ttagtaatcg ttgtaaaatc attcgcagca gtcacttcaa taccttgctc
     8941 tttcgcttgt tttaaaattg aacaaatacg cgcatgtgca tattgagcat agtaaactgg
     9001 attgtcttga gattgctctt tcgctaattc catatcaaaa tcaaagtgac tatcaggact
     9061 acgcatagtt aagaaataac gtgcagcgtc aacgccaact tcgtccataa tttctcttaa
     9121 tgtaatcgca ttaccagtac gtttactcat cttcacttct ttaccatttt ccattaaacg
     9181 aaccatttgc atgatttgaa tttctaaacg attactatct acaccaaacg tttcaagaga
     9241 tgctttcaaa cgattaatat aaccatgatg atcagcacca aataaatcga ttaaaatgtc
     9301 attaccacgt tttactttat cgaagtggta cgcaatatct ggtaagaaat acgtatatgt
     9361 accgtcattt ttaattaata ctctgtcttt gtcgtcttta aaatcagttg tacgtaacca
     9421 tgtagcgcca tcagcttcat acgtataacc taattctttc atttttgcta aaacttcaag
     9481 aatttctcct ttttcatata aagatgtttc actaaaccaa ttatcaaaat gcgtattgaa
     9541 ctctgctaaa tcatttttca atttagccat ttcgtattct acgcctaatt ttctaaattc
     9601 tttcaaacgt gcttcttcag aataatcttt aatttcaggg tgtttctctg ctaaatcttt
     9661 acctatttca ataatatctt ttccattata gccatcttct ggcattgaat aactattgtc
     9721 acctaaagct tcaaagaaac gtgtttcaat cgaacgcgct aagttagtaa tttgattacc
     9781 agcatcatta atataatatt cacgtgttac attatagcca gctgcagtta aaatattagc
     9841 taaagcatca ccaactgctg catttctagc atgaccaata tgtaaatctc ctgtagggtt
     9901 agctgaaaca tactcaagca atacattttg accttttgat tcatttacat gtccaaattg
     9961 atcacctttt tcaattgctt caggaataat tgctgttaaa tactgattat ctaagtaaaa
    10021 attaatgaat cctggaccag caatgtcaat ttgttttaca tgtgcttttt cagtatctaa
    10081 gttatcaaca atcgcttgag caatttcacg aggattacgc tttgcaatct tagtcagtac
    10141 catcgcaata ttagtagcat aatctccatt ttttgtatct ttaggaactt caattttaat
    10201 atcaggaatc tcatctgcta atcctgcttt gttaatactt gctgcaattt cttctactaa
    10261 tgtttgtttc acttgatcaa taatattcat tcgcctatct ccttatagtt aatttcatat
    10321 tgataagaac ccattttttc attatcttga tataattcat aatgaatctt tagcttgcca
    10381 ccgtctccac ttacgaaatg taaaatgcgt aatgttttaa cttctagtgg aattcgtcca
    10441 gccgatatat cgtaaaaagt tgttgtcgtt tgtccttcaa caaaatgcaa attcatatta
    10501 atgtcgcctt tacgaatcaa cttaacgcta tcatcatcca cttttattgt aacattaact
    10561 gttgcatcct caatttgttc tacataccga ataaagttag attgcctttg ttgccaagtt
    10621 ccttcagtag taaattcaaa tttttctttt tcgttgtgct gtttcaacac ttgctttgtt
    10681 tgaatactta cttttttatc caatttacta ctcaccttaa tcttcgcata atagaaatat
    10741 tatagcataa aataaggtca tcttgttatg ccttcttaaa gctaaatagg ttcaattaat
    10801 taacaattcc atacttaatc aacgcttcat atatgtaagc cattttataa aatacaaaca
    10861 acacctcaaa accaaaaaac acgagatttt cactatcttt catgaaaacc tcgtgtttga
    10921 ttataatgat agtgttcaga gcactacctt ctttatctat atagtcagtt gatattttta
    10981 aaatttaata accattctac cagttatagt accattttcc atttcttcaa aaatatcatt
    11041 gatttcttct aattttctta attgaacttt aggtgttact ttattttcag cagcaaattc
    11101 aaacgcttca cgtaagtctt gtcttgtacc aacaagtgaa cctactactt caataccatc
    11161 aagcactaat cttgggatat ctaagttcat tttatcaaca ggtaaaccaa cggcaacaac
    11221 tcttgcacca gcttttacaa catcaaccgc ttggttaaat ggcgtcttag caactgaagt
    11281 taccactgtt gcatctaatc ctttgttatc agttaatttc ataacttctg caactggatc
    11341 aacatcttta gaattaataa tagcatcagc acctaattct ttcgcgaatg ctaatttatc
    11401 atcattgatg tcgaaggcaa caattttagc ccccataacg tttttagcat attgtaaagc
    11461 taggttacct aaaccaccta taccaaaaac acctaaccat tgtccaggtt ttacattact
    11521 tacttttaca gctttataag ttgtcacacc tgcgcatgta atagaagacg ctgctgctgg
    11581 atctaatttt tcaggtactt tcacagcata gtctgcagta acaataactt gttcagccat
    11641 tgcaccatct actgtataac cagcattttt cacactacgg caaagtgttt cacgacctgt
    11701 tgtacaatat tcacatcttc cacagctttc gaacatccaa gcgatagaca cacggtctcc
    11761 aatttttaat gattctacat cttccgcaac ttcgatgact ttaccaatac cttcatgacc
    11821 taaagtaacg cctgtaacat caccaaaatc agcattctta acatgtaaat cggtatgaca
    11881 aacgccacaa tattccgttt gtaccaacgc ttcaccaggt tttaaagctc ttaacttttt
    11941 gtcctcaata cttactttgt gatctttcgt tacaactgct gctctcataa taatgtcctc
    12001 cttatagtca ctccagtatg tatgatatta attggctatt actttcaaaa aagaaaacga
    12061 ataattatag atgtaatttt tatttgtaat agacgccaga ttaaaacata ctactgtgac
    12121 cttgttttta gttagatatc aaccgttaac ttttaacgtt tccctagtta atatgttaaa
    12181 agaaatgaca taatcgatat gctaacgtgt aatttttact tcacatatat ttatttaaag
    12241 ataatttata atactcttat cttcactctt attataatgt gaattaattc acaaagtata
    12301 tagctatgat caaaaaaatg tgtcagttat tcaaattttt tattaactac agattaattt
    12361 aatttcaaaa aaattaaaaa aactcccaat caatctaaga cattattttc acctcacgat
    12421 ttacgtcgtc attgagaaat gaatcatatc tttatagatt aattgggaaa ttttagtttc
    12481 taatatttaa ttaaatgctt gatgtaaagc atcagaagaa tgcttccaca taccttcatc
    12541 atggttcatt aaaaagtctt ttaaaatagc tttattctcg tcaccaattt gatctatgat
    12601 aatatgatgt ttcattgatt tatccatcat attaacatgt tcaggcatac ttttataacc
    12661 acgacgaatt gctttattca ctaataaata gcacgctgtt aaaccagcgt aatatggtcc
    12721 ttgttcatta cgttctgttg ttacccaaca caaccaatat tctttagctt catcgccttc
    12781 aacagcagct ttatcagtaa tccatttcac acctttttca actttggcac gagcatgcat
    12841 gcctccaata tcaataaatg cttctttgtt agcaacatcg ataaaaactg gtgcaatatt
    12901 gtctaagcta attgaaccaa tatttgtacc tttatgacca tctagagggt cattttttat
    12961 gatattaaaa ttaaagcctt ttttctcaga caatgatata acctccatat ttaacgataa
    13021 caattttagc cctgtttaat taagctcatt aatcaaattt tcaatagctt ctctaattgt
    13081 cttatcttta atctttgcaa ccatttctct aggataatat aatttatagt cttggcgatt
    13141 aatgcctgta atactatgaa tgactaatga ttgattgctt atttctctaa tcgtaccatc
    13201 ttgtcttaat aaatgaatcg gtttgcgatt tgaccccggt cgatcatagt catatggcaa
    13261 atcagaaaat gcttcactca caaaataata atctggatta ataccacctg cttcaaacag
    13321 ttcttgcagt tctgatattg taataattga gccatcaaat ggaatatatt taaataagtc
    13381 tcgattaata aatcgacttg ctaaatcact taaaatagca tcatcttctt ttgtccattt
    13441 ttccaaataa tacgtaacta ccgcttcatc gagttcaaca tattgttcaa tcgtaactgt
    13501 ctcttcaaaa aatggaataa aatcatgtgg atgcaactta aattcatagc cttcattata
    13561 aagctgtttt gcgcgtttca aacaattatt aagcagcact tctccaccac gactaactgg
    13621 gtggaagtaa atttgccaat acatttgata acgactcata ataaagtttt caactgcatg
    13681 cataccactt tctttgatta gtacttcatc tttagaaggt cgcattaatc ttaaaatacg
    13741 ctccatatca aaagcaccat atgatacacc tgtgaaatac gcatcacgtt gtaaataatc
    13801 cattctatcc gcatcgattt gtgacgaaat catcgaaatg accaatttat tatgatgcgt
    13861 tttattaatt acttccgcaa cttctcttgg aaactcaggc gccactttac gtaatacagc
    13921 attcacctca gtatctccag taataatcgc ttgtgtgtat gcttcatggt ctgtattaaa
    13981 tattttttca aaactatgtg aaaatggacc atgtcctaaa tcatgtaata atgcagcaca
    14041 taatgccaac ggtctatctt tattgtccca tgcatcatga ccaataaatg actcatcaat
    14101 taatcgtcta actatttcat acacacctaa agaatgtcca aagcgactat gttctgctgt
    14161 gtgaaaagat aggtacagtg ttcctagttg tctaattcga cgtaaccttt ggaattcctt
    14221 agttttaatt aaatcccata tcaattgatc ttcaacatga atatatcgat gtatcgggtc
    14281 tttaaaaact ttttcttcta ctaattttaa atctacatat gcgttagtca ttattcccct
    14341 ccttttcgtt taatataata tttaatttac ttaaaatgct ttgtacataa gtgctaagtc
    14401 taacttttcg ccatacattt ctggctcata agagcgtaag attgtaaaac cttgctcttt
    14461 atagtaagct actgcttctt catttttatt atctacttct aagtaaacac cttcaaattt
    14521 atcttcaaaa cgtgataatc cttcatttaa caatgctgta ccataacctg tatgttgcga
    14581 ttctggttta acataatgag ctgataaata taattcttca ccgtaaataa agttagcaaa
    14641 gccaacgatg tcattacctt cttcaacgac taagaataat tgttcttgaa gtcttttctt
    14701 taaatgatgt tcattatatg aagcttctaa caagtgatta actgttgtcg cagcgtatat
    14761 atttaagtat gtattaaacc aagctttagt tgcgacatct ctaatttgaa caacatcttt
    14821 ttcagttgct tgtcttacct tgaacatgac tttctcccct tattaacaag ttttaataac
    14881 ggcattatac cacaacttgc tcaatactta ataaacaatg attgtctatt caatttatat
    14941 atttatattt tccgttaaaa ttaaaaataa aaaataacga agcaaaaaat cacttcgttt
    15001 agtatgaggt atgtcttatt gcaatatact attccactca gttgcacgtg ctaaggcata
    15061 gttgtctttc atgatgtcac caggcttttc agcagttcca ataatataac catttaaagt
    15121 ggcacctaaa aagtctaaac tatatttcat ttgcgtaatt gctggcttcg cttttatttt
    15181 tggacaatct ccaccaacta aaataactct aaaatccttt tcggccattt gtgccttaaa
    15241 attaggatat cgtttatctt gtaatgtttc tgaccaatgt tcgataaatg ctttcaatgg
    15301 tgctgaaatg ctataccaat acactggtga tgcaaaaata attgtatcac tagccaatat
    15361 tttatctaga atcggcaaat agtcatcgtc atatgaagta atagtctctg ctgtatgtct
    15421 cacgtcacgt atcggtttaa actgatgttg tgtcacgtca atccattgat actctaaatc
    15481 ttgcaaagcg aattttgtta attgtgcagt attaccgttt ggtctactcc caccaaacaa
    15541 aacagtaatc attttagcct aacctcactt ttgattaata aatatctgtg tttttcgtta
    15601 cctaattata ctatcataag ctttgcctac cgaatagtaa aacgcttaca acttttatat
    15661 aaatttgacg aaatttcgtc atgccttata taacgtcgtt tgtgatacgg ggctaattca
    15721 tgatgaaatt agatacatat atcaccatta aatacaattc atttagtctt caatcggaaa
    15781 cagttcatcg atatattgaa tctcatcatc tgataaaacg atatctgcag ctttaatatt
    15841 ttcaacgact tgttctgcac gttttgcacc aggaataatc acatcgatag ctggtctcgt
    15901 taaataaaat gctaatacaa tgttcgcaat tgaagtttga tgtgctgcag ctatgctttc
    15961 caaagctttt acgcgacgca cattttcttc aaatacacct ggtttaaaat cacgacgtgt
    16021 agtacgatgg tcactaaatt tagtgttctc atcatatttt ccagctaaaa taccggatgc
    16081 taatgggaaa tatggaataa atgtgatttg gtgatcaaca caatattgta atactgcctc
    16141 attttcgcga tgcaataaat tatattctaa ctgtacaaca tcaacgtaac catctttatt
    16201 tgcttcttta agttgatcta atgtgaaatt tgatacacca attgctttaa tcttcccttg
    16261 ttccttaagc tcttgtaatg ctgcaactgc ttgatctttc ggagtgttgt tatccggaaa
    16321 atgaatataa tataaatcga tataatcagt ttgtagacgt ttcaaactat tctcaacttg
    16381 ttgttttaaa tattccggtt gattgttctg atgtacttct tgattttcat caaattcatg
    16441 agaccctttc gtagcaattt taatttgctc tcgcggatat tctttaacaa cttctccaac
    16501 caattcttct gatcgttctg gcccataaat atatgccgta tctaataaat taataccatg
    16561 attaatggct tgacgaacaa catcttttcc ttgttcttca tctaagttcg gatataaatt
    16621 atgcccacct actgcgttcg tcccaagtgc gattggaaac acttcaacat cagatttacc
    16681 taagtttaca aattgcttca ttagacccag ctccttatct ttataacttt tccactatca
    16741 cactatcatg atttaataca acttccaaat gttagaatta gagcttgaat ttaagcggtg
    16801 taataggaaa acgttatata aaactcagtt aaaataagta ttgcttaaat tgatatgcat
    16861 atagcgcgat tgctcgttga cctttaaatt acatgtactt ttgaatcaga aattgaaaaa
    16921 catttaagaa cgacaatatt aaagccattg caaatttaat ttggtaattt ctattggttt
    16981 ttaagtatga catgaatgtt tttatctata attaaagcta tattaactag aaaaaaaccc
    17041 attataactg caatccaaac aaaatatact attatgtttt gataactcat tgataatagt
    17101 gcatctagga atagcatagt caatccgcca aataaaatat atccaaataa tgtgaaacaa
    17161 acatttttta gtgtaggtat taaaaaaatt tttgaccttt tatttttagt attataaata
    17221 tttaaagatg attttttaat taggcgtaca taaaatatta aaatccctat aaatgtgatg
    17281 caacatatgg caataactaa ttttttatca agttgaatgt caagtaaatg tgtatatttt
    17341 cttaatgcaa ctcccagcaa agcagagcta cctgcaactg gccaaaaaac attcttttta
    17401 gcaggtttaa caatatttaa gttttcaaac tcttctctac taattttgac gtattctttt
    17461 gggatgaacc aattaataaa aggaaaaaat aaactaatcc aagtgcttat tatatcgacc
    17521 attaagtatt catcattata tttaataatt ctatattttg ggtttttatt gatgatttta
    17581 gattcgcata gcaacattta ccagctccat ttcttagggc ttatatgatt agaaatactt
    17641 tttgaagctt caccatgaca ttttcaacac catctactgt tttttctgca gatttttcta
    17701 cagcatctat accaacgtct agtttatgtc ctatccaatc gtttttatct tttattccaa
    17761 attcattttt ttgattggtc caatatacaa gtattgccga aatagaacct gcagtaaatc
    17821 cagctaaacc atcatttttc caccgtgtta gttttagata atacataaat ttttttgtca
    17881 ataatcgatg acatgtttaa cagaaagaaa cctgcagtca ttaccaacca accaataaag
    17941 cctattatat tttgaggatt taaagttaat aacatactta gagccattat tgaaagaccg
    18001 ccaaataaaa aataataaaa tattgtgaaa caaaagtttt ttaatgaagg aaataaaatt
    18061 attttccctt tactagagtt attattataa atttccaacc ttagcttacg attcagaaat
    18121 aaaaaaagta ttaaaacacc cagaaatatt gcacaacata ttacaatcac catgtttttc
    18181 tctaattgaa tattaagtga aggaatatat tttctaaacg taactccgaa caaaatagta
    18241 ctacctgcaa ctggccaaaa agctttattt ttaacaggtt tgacaatgtt taaatcgtca
    18301 aattcttttt tactgatttt gacatatttt tttggaatta accaattaat cataggaaga
    18361 aataaagtta accaggtact tactaaatca atcattaaat attcatcacc atatttaata
    18421 actctatact taggattttt gttgataact ttagattcac atagcaatat ttatcactac
    18481 cattcctttg tttattattc ctattaaaac ttttcaattc catttgattt aaataataca
    18541 tgaactttat tgtcatatag tgagcacata tttaaaaata agaaaaacaa aaatattaca
    18601 aacaaagtta taaataatat tatattttga acttcaatcg tcaataacat ataaatagta
    18661 aagaatgaac caccacctaa ataaatatat aaaactattg ataaacaaag actctttaaa
    18721 gtgggaaata tgattatctt attgctttta tttttgtttt cattatagat ttgtaacttt
    18781 aacttttgat ttaaatat
//
LOCUS       DANJFZ010000048        17963 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.048, whole genome shotgun sequence.
ACCESSION   DANJFZ010000048 DANJFZ010000000
VERSION     DANJFZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.048"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            37..3501
                     /gene="sdrE"
                     /locus_tag="PWN45_001448"
     CDS             37..3501
                     /gene="sdrE"
                     /locus_tag="PWN45_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HDL0623217.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLF
                     GRRKKQNK"
     gene            3620..5092
                     /locus_tag="PWN45_001449"
     CDS             3620..5092
                     /locus_tag="PWN45_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0623218.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5216..6706)
                     /locus_tag="PWN45_001450"
     CDS             complement(5216..6706)
                     /locus_tag="PWN45_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0623219.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6988..7866)
                     /locus_tag="PWN45_001451"
     CDS             complement(6988..7866)
                     /locus_tag="PWN45_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDL0623220.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(7879..8544)
                     /gene="bshB2"
                     /locus_tag="PWN45_001452"
     CDS             complement(7879..8544)
                     /gene="bshB2"
                     /locus_tag="PWN45_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDL0623221.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(8558..8920)
                     /locus_tag="PWN45_001453"
     CDS             complement(8558..8920)
                     /locus_tag="PWN45_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDL0623222.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            9196..9954
                     /gene="nagB"
                     /locus_tag="PWN45_001454"
     CDS             9196..9954
                     /gene="nagB"
                     /locus_tag="PWN45_001454"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL0623223.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            10031..10663
                     /gene="hxlA"
                     /locus_tag="PWN45_001455"
     CDS             10031..10663
                     /gene="hxlA"
                     /locus_tag="PWN45_001455"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDL0623224.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            10665..11213
                     /gene="hxlB"
                     /locus_tag="PWN45_001456"
     CDS             10665..11213
                     /gene="hxlB"
                     /locus_tag="PWN45_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDL0623225.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            11326..11973
                     /locus_tag="PWN45_001457"
     CDS             11326..11973
                     /locus_tag="PWN45_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0623226.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            12475..13875
                     /locus_tag="PWN45_001458"
     CDS             12475..13875
                     /locus_tag="PWN45_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623227.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            14419..15795
                     /locus_tag="PWN45_001459"
     CDS             14419..15795
                     /locus_tag="PWN45_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDL0623228.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            15797..16936
                     /locus_tag="PWN45_001460"
     CDS             15797..16936
                     /locus_tag="PWN45_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDL0623229.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            16911..17276
                     /locus_tag="PWN45_001461"
     CDS             16911..17276
                     /locus_tag="PWN45_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDL0623230.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            17279..17548
                     /locus_tag="PWN45_001462"
     CDS             17279..17548
                     /locus_tag="PWN45_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623231.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(17749..17916)
                     /locus_tag="PWN45_001463"
     CDS             complement(17749..17916)
                     /locus_tag="PWN45_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDL0623232.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         6488 a         2735 c         3161 g         5579 t
ORIGIN      
        1 ttttttgcaa atattgatat tttaaaggag atttatatga ttaacaggga taataaaaag
       61 gcaataacaa aaaagggtat gatttcaaat cgcttaaaca aattttcgat tagaaagtat
      121 actgtaggaa ctgcatcgat tttagtaggt acgacattga tttttggtct agggaaccaa
      181 gaagctaaag ctgctgaaaa cactagtaca gaaaatgcaa aacaagatga tgcaacgact
      241 agtgataata aagaagtagt gtcggaaact gaaaataatt cgacaacaga aaataattca
      301 acaaatccaa ttaagaaaga aacaaatact gattcacaac cagaagctaa aaaagaatca
      361 acttcatcaa gtactcaaaa acagcaaaat aacgttacag ctacaactga aactaagcct
      421 caaaacattg aaaaagaaaa tgttaaacct tcaactgata aaactgcgac agaagataca
      481 tctgttattt tagaagagaa gaaagcacca aataatacaa ataacgatgt aactacaaaa
      541 ccatctacaa gtgaaccatc tacaagtgaa attcaaacaa aaccaactac acctcaagaa
      601 tctacaaata ttgaaaattc acaaccgcaa ccaacgcctt caaaagtaga caatcaagtt
      661 acagatgcaa ctaatccaaa agaaccagta aatgtgtcaa aagaagaact taaaaataat
      721 cctgagaaat taaaagaatt ggttagaaat gatagcaata cagatcattc aactaaacca
      781 gttgctacag ctccaacaag tgttgcacca aaacgtgtaa acgcaaaaat gcgctttgca
      841 gttgcacaac cagcagcagt tgcttcaaac aatgtaaatg atttaattaa agtgacgaag
      901 caaacaatca aagttggcga tggtaaagat aatgtggcag cagcgcatga cggtaaagat
      961 attgaatatg atacagagtt tacaattgac aataaagtca aaaaaggcga tacaatgacg
     1021 attaattatg ataagaatgt aattccttcg gatttaacag ataaaaatga tcctatcgat
     1081 attactgatc catcaggaga ggtcattgct aaaggaacat ttgataaagc aactaagcaa
     1141 atcacatata catttacaga ctatgtagat aaatatgaag atataaaatc acgcttaact
     1201 ctatattcgt atattgataa aaaaacagtt ccaaatgaga caagtttgaa tttaacattt
     1261 gctacagcag gtaaagaaac aagccaaaat gtcactgttg attatcaaga tccaatggtc
     1321 catggtgatt caaacattca atctatcttt acaaaattag atgaagataa gcaaactatt
     1381 gaacaacaaa tttatgttaa cccattgaaa aaatcagcaa ccaacactaa agttgatata
     1441 gctggtagtc aagtagatga ttatggaaat attaaactag gaaatggtag caccattatt
     1501 gaccaaaata cagaaataaa ggtttataaa gttaactctg atcaacaatt gcctcaaagt
     1561 aatagaatct atgattttag tcaatacgaa gatgtaacaa gtcaatttga taataaaaaa
     1621 tcatttagta ataatgtagc aacattggat tttggtgata ttaattcagc ctatattatc
     1681 aaagttgtta gtaaatatac acctacatca gatggcgaac tagatattgc ccaaggtact
     1741 agtatgagaa caactgataa atatggttat tataattatg caggatattc aaacttcatc
     1801 gtaacttcta atgacactgg cggtggcgac ggtactgtta aacctgaaga aaagttatac
     1861 aaaattggtg actatgtatg ggaagacgtt gataaagacg gtgttcaagg tacagattca
     1921 aaagaaaaac caatggcaaa cgttttagtt acattaactt acccggacgg tactacaaaa
     1981 tcagtaagaa cagatgctaa tggtcattat gaattcggtg gtttgaaaga cggagaaact
     2041 tatacagtta aattcgaaac gccaactgga tatcttccaa caaaagtaaa tggaacaact
     2101 gatggtgaaa aagactcaaa tggtagttcg gttactgtta aaattaatgg taaagatgat
     2161 atgtctttag atactggttt ttacaaagaa cctaaataca acttaggtga ctatgtatgg
     2221 gaagatacta ataaagatgg tatccaagat gcaaatgagc caggaatcaa agatgttaag
     2281 gttacattaa aagatagtac tggaaaagtt attggtacaa ctactactga tgcctcgggt
     2341 aaatataaat ttacagattt agataatggt aactatacag tagaatttga aacaccagca
     2401 ggttacacgc caacggttaa aaatactaca gctgatgata aagattctaa tggtttaaca
     2461 acaacaggtg tcattaaaga tgcagataat atgacattag acagtggttt ctataaaaca
     2521 ccaaaataca gtttaggtga ttatgtttgg tacgacagta ataaagacgg caaacaagat
     2581 tcaactgaaa aaggtatcaa agatgtgaca gttacattgc aaaacgaaaa aggcgaagta
     2641 attggaacaa ctaaaacaga tgaaaatggt aaatatcgtt tcgataattt agatagcggt
     2701 aaatacaaag ttatttttga aaagcctgct ggcttaacac aaacagttac aaatacaact
     2761 gaagatgata aagatgcaga tggtggcgaa gttgacgtaa caattacgga tcatgatgat
     2821 ttcacacttg ataacggata cttcgaagaa gatacatcag acagcgattc agactcagat
     2881 agtgactcag acagcgactc agactcagac agcgactcag actcagacag tgattcagat
     2941 tcagacagcg actcagattc agatagcgac tcagattcgg acagcgattc agactcagat
     3001 agcgactcag attcagatag cgattcagac tcagacagcg actcagattc agatagcgat
     3061 tcggactcag acagcgattc agactcagat agcgactcag actcagacag cgactcagat
     3121 tcagatagcg attcggactc agatagcgac tcagattcag acagcgattc agactcagat
     3181 agcgactcag actcagacag tgattcagat tcagacagcg actcagactc agatagcgac
     3241 tcagattcgg acagcgactc agactctgat agcgactcag actcagacag tgattcagac
     3301 agcgattcag actcggatgc aggaaaacat acacctgtta aaccaatgag tactactaaa
     3361 gaccatcaca ataaagcaaa agcattacca gaaacaggta gtgaaaataa cggctcaaat
     3421 aacgcaacgt tatttggtgg attatttgca gcattaggtt cattattgtt attcggtcgt
     3481 cgcaaaaaac aaaacaaata atacaatatg acccaggtcc ttgtggcctg gtttttttat
     3541 aattacacat gcaatagatg tatttttcat ataaaataaa caataaagat acggaataaa
     3601 acttatatga ggcgataata tgaattacat tttaggaaca attttagaaa gtaaaattac
     3661 aggtgtagaa aaagcgcaaa taaatagatt gaagttgttc aaacaacacg gcatatcttc
     3721 aaaatgtgta tatgttaaat ggaatcctta ttcatacaca tatgcgaagc aacatcagat
     3781 tgaaaatgat gtatttacaa tgtatgacta ttttcaaaaa gcaatcaatt ataaaaagac
     3841 aaagcaagtt aactggatac agtattggga aaagtcatgt aggtacacat tgaaatttgt
     3901 ggaaaattca aatgatgtca gaatatatga tgaagagcaa tttgtaatgt atgctcattt
     3961 tttagataaa cagtatcatc aattaaatta tgtgaattat tttgatcata aaagaagaaa
     4021 agtaaaacgc gaattgtatg atggaagagg ctttttaagt tgttctcgaa ttttaggtga
     4081 aggacaacgg attgtactcg aaaattacta tacacctaat ggggaaatcg tcatccaaaa
     4141 atattttgac gatataaaag ggaaaaacac gctcacaaag gttatcttaa atgaagacca
     4201 gcatcaacaa ttttttgata cagaagatga attagttcaa tattttctcc atcaattatg
     4261 taaaaataat gatcaaatca tattagatcg tcctcatgaa ttaggaaatg ttatagcggg
     4321 attaaatcaa agtattccag ttattgttgt actccatagt acacatttat ccggtgccgg
     4381 taatggtata aaaagttttt ataaaacagt gtttaataat ttaacacgtt ataaagcgat
     4441 tgttgtatca acagaaaagc aatgccaaga tatttcacaa tatattgaaa ataaaatacc
     4501 agttatcaat attccggttg gctacgtggc aaatttaaag tatcaatttg acatcaatca
     4561 aaaggagaaa aatcatatca tatcaattgc tcgcctcgtt gaaaataaac aaattaaaca
     4621 tcaaattgaa gtaatcaagc aattagtaac aaaacatccc aatattcaat tgaatattta
     4681 tggacatgga aatggtttgt cagaatatcg acaacttgta gaagattatc atttatcgga
     4741 acatgttaaa tttcatggtt ttaagacgca tattaatgaa gagattgcta aagcagaact
     4801 gatgttatcg acaagtaaaa tggaaggttt tggcttagca attttagagt cgctttcagt
     4861 aggtacacca gtgatcagtt atgatgtaga ttatggtcca tcagaactga ttcaagatgg
     4921 atttaatggc tatttagtac ctcaaggtga catcaatcaa atggttgaaa aggtcgacca
     4981 attactaaat aatactcaaa aattgcaaca gttttcaatt aatagcatag aatctgcaca
     5041 acagtacaat gcaactacta tcagtacaaa gtggcaaaat attttaaact aagtcaaaag
     5101 aaaaaagcat tttcctagaa tcatctattt aacaaacctc gttcatacga acttacatgt
     5161 ttagttaaat gacataggaa aatgctattt ttgtaaaaat aaaggtaaag tacatttata
     5221 taagacgaac aaattggtcc cattgtttaa ttaacgacgc tttactatat tgttgtgctt
     5281 ttgccaaact accttttgac agtcgttgct gtacttcagg atgatcaatc acatatttta
     5341 ctttatcaaa tagggcatct tcatcatttt tagtaattaa ataaccattg aaatctgaag
     5401 taatcagttc gttaggtcca tatttaatat cataactaat aactggaaca ccatgtgcta
     5461 aagattcaag tagcgctaaa gagaaacctt ccatgttact tgttattaaa ctcaaatagg
     5521 catcgctata ttcttggtct agattgctta aaaagccgcg taagtaaaca tgattttcca
     5581 atccatattt ttgtatcaat tcatttaatt ttttactttc agaaccaaaa ccatacatat
     5641 gaagctctat ttttgggaca tacgatacta agcgtttaat taattcaatt tgttgatgta
     5701 attgtttttc aggtgaataa cgagcaacgg aaattaattt aacactgcgc tgatctaatg
     5761 tttggactgg tgtatcaatt gtttcactat agccgacagg aatattaaca actggaatag
     5821 tatggttaat acgtttttca acatctaatt tttgctgctc agtagaaacg ataattgcac
     5881 gatatcgaga taaattttca aacatcgctt tatatacatt tttaaatggc gatgaatcta
     5941 atgcatcaat atttttaatg tgtgtactgt gaagcacagc tactactggg attgactcag
     6001 gcgttaagtt gaaaataggt gctgtgtaca cattacgatc actgaaaaat aaatccccat
     6061 gttgatatag ttgtttaatg aaaaatgcgc ctaattccgt ttcattatta aagaaatatt
     6121 gtttgttagc atagtaaaca ataatttttt gtacttctgg tttgccatcc ttgtaagaaa
     6181 aatacttttc taattttgtg tcaccttctg gattatagaa aaattcacat aatgtttgtt
     6241 gtttatcaac aagaatccta ctacaactta aaaagccacg cacatcataa aaatcacgtt
     6301 ttacttttcg tctttgacta tcaaaatgat ttacataatc taatatacga tatttaggat
     6361 cttgaaaatg ggcatacatt aagaaacgct cttgatcata tattctaaag tcatgactat
     6421 tttcaacatg ttttaaagta taatgacatt catcagtcca atacgacaac cagtcaaatg
     6481 gttcattgcg ttctaaatat gttgcttctt ggaagaaatc atacatatta atatagtcag
     6541 aactagtaat ataattttgg gcatttctat ataaatatct attccatgac agaaatacac
     6601 attgcgctgg tcttcccatt tctttaaata aatttaaacg attaataatt gctttctcta
     6661 tcccagttaa attaacacct aaactattac ctacaaaata attcatttac aacaccactt
     6721 atatctattt tttataatta tatcacataa tatttaatta cttcttttaa ctggaagatg
     6781 tgtttattta taaaacaaca aattttgata tttataatga tagtagttat tcaatcaact
     6841 acgaccaata tatcattgta gagcttagga tattgattta tgactcaggc acatcaaatg
     6901 agaagattta taaaagagat atacaactct agaaggtata ataaaaacgc gcaactaatg
     6961 ttacgcgttt gaattaatca tatgatatta tttgcgatac tttaatttag cgaaagcatc
     7021 atgttgatgg atagactctt cattacgaca ttcgatatcg aaaccgtcta accaatcaaa
     7081 ttcaactaag tccgcggcaa ttaaacgaat taagtcttcg acaaaacgtg gattttcata
     7141 tgcacgctct gtcacacgtt tttcatcagg acgttttaaa atagggtata gaattgaact
     7201 tgcattagct tccattgcat ctaaaatttt atttttatag tcatcaacta tgtcttgatc
     7261 tttattaata tatgttttaa cagtgacaac accacgttgg ttgtgcgctg aatactcact
     7321 tatttctttt gaacaagggc atagcgttgt gacagttgct tcaatagtaa gttctttacg
     7381 tgtaacttta tcaccgtcaa ttgctaatcc ataagtgaca tcggcattac caactgcttt
     7441 aatatttgtg gttggactat agcgatcaaa gaaccatttc ccagaaacat caacgcctgc
     7501 cgcattttgt ttcatattcg tttgtaaagt gcgtaacacc tgataaagtg tattaaattc
     7561 aagttcaata ccattatcat agtgcttttc aacactttcg attatacggc tcatattaat
     7621 acctttttcg tcttttgtta aacttgttga aaaactaaat gtgccagctg tttgatactg
     7681 gtcaacaagt acagggtaca ctaagttttt aataccaact tcttctattt caaataaaaa
     7741 atctttatgt gtactttgta aatctgtcat ttcgttctta gtagtaggtt tcgtgccttc
     7801 aataggatct acggaaccaa agtgtttcca acgaccttct cgtgtcgata aatcaaattc
     7861 agtcattttt ttcctccgtt aagatttaaa gtgatatgtc caatatggtt cgactgttaa
     7921 aaagctgtgt tgtttaccat cgatttcagg acttgctaat tgttttaaaa atggacctgt
     7981 ttgagaagca tgtgcttcaa atgccttaat tttaagttct ttaaaatctg taatatcatt
     8041 ttgaatatca ggttctccaa gagcttcggt tgcatcatta ctgaacgcaa ctaaagttaa
     8101 acgagggcgt tcttctttag gcatgcgttc aaccgttcga attacagcgt ctgctgttgc
     8161 ttcgtgatca ggatgtactg catatccagg ataaaatgaa ataatcaatg atggatttgt
     8221 atcatcgatt aaagatttaa tcataccatc tatatgttca tagggttcaa attcgacagt
     8281 tttgtcacgt aaacccattt ttcttaaatc agtaataccg ataactttac aagcttcttc
     8341 tagttcacgc tcacgaatac ttggtaatga ttcgcgtgtt gcaaatgggg gattacctaa
     8401 atttctgccc atttgtccta gggttaaaca tgcatatgtt acaggtatgc ctttttggat
     8461 ataatttgct aatgtgcctg cagatgagaa ggtttcatca tcaggatgtg gaaatattac
     8521 taatacatgt ctttcgtcag tcatgttgat gcctcctcta taaattaaat ggtcgctcac
     8581 taatttgaag tgctgcagcg agttgacctt cgtaattaaa acctgcaatt aaaaattcat
     8641 catgctcatt gacctcaaaa tgcgttagac cttgtacata aacccaacca ccatttgata
     8701 gtttaagacc aatgcgataa ggttctttat taccaccttt tagttgtgca tgcgtatatg
     8761 ttatttgtat gtttcttaaa aaagtaccag cattaaaaac acgttgatcg aaatggttcg
     8821 cataggcccc atttgtcgtt tcaacatgca gatacacagg tttatgttca aaagaagcaa
     8881 gtaaatctat aacttcttgt tctttaattg gttccaacac gttcactcct tacactatca
     8941 atgtgtttat ctttctattt tactaaaaac tattcgataa ttgtatacga ttgctcaatt
     9001 atttataaat taattttcat gaagggtaat tactcaggat tacgtaatca tacagcatta
     9061 gttttttact tttaaaaatc aaaaatttgt tggaatttga aaagtgttaa acattaaaaa
     9121 tgatgctata ttaatggtgt atgaatgaat tcataagttt ttaaaatgta ttaaatttgt
     9181 ggaggcatgt aaacaatgaa agtattaaac ttaggatcga aaaaacaagc atcattctat
     9241 gttgcatgtg agttatataa agagatggca tttaatcagc actgtaaact aggtttagca
     9301 actggtggta caatgacaga tttgtatgaa caacttgtta agttattaaa taaaaatcag
     9361 ttaaacgtag acaatgtatc cacgtttaat ttagacgaat atgtaggttt aaccgcatca
     9421 catccgcaaa gttatcacta ttatatggat gacatgcttt tcaaacaata tccttatttt
     9481 aatagaaaga acattcatat tccaaatgga gatgccgatg atatgaatgc ggaagcgtca
     9541 aaatataatg acgttttaga acaacaaggt caacgtgata ttcaaatttt aggtattggt
     9601 gaaaatggtc atattggatt taatgaacct ggtacgccgt ttgatagcgt tactcatatc
     9661 gttgatttga ctgaaagtac tattaaggct aatagtcgat attttaaaaa cgaagatgat
     9721 gttccaaagc aagccatttc gatgggactt gctaatattc ttcaagccaa acgtatcatt
     9781 ttactcgcat ttggtgaaaa gaaacgtgct gctattacac atttattaaa tcaggaaatt
     9841 tctgttgatg ttccagccac attacttcac aaacacccga atgttgagat atatttagac
     9901 gacgaagctt gcccgaaaaa tgttgcgaaa attcatgtcg atgaaatgga ttgattgcaa
     9961 tgtttaatta agaaatgcct cgggaaaggt tccaatagaa agataaaaag cattggaagg
    10021 atgattttta gtggaattac aattagcaat tgatttatta aacaaagaag acgcggctga
    10081 gttagcaaat aaagtaaaag attatgtaga tatcgtagaa atcggtacgc caatcattta
    10141 caacgaaggt ttaccagcag ttaaacatat ggcagacaac attagtaatg taaaagtatt
    10201 agcagacatg aaaattatag atgcagctga ttatgaagtt agccaagcaa ttaaatttgg
    10261 cgcggatgta attacaatac taggtgttgc agaagatgca tcaattaaag cagctattga
    10321 agaagctcat aaaaataata aacaattact agttgatatg attgctgttc aagatttaga
    10381 aaaacgtgca aaagaactag atgaaatggg tgctgattat attgcagtac acactggtta
    10441 tgatttacaa gcagaagggc aatcaccatt agaaagttta agaaccgtta aatctgttat
    10501 taaaaattct aaagttgcag tagcaggtgg aattaaacca gatacaatta aagatattgt
    10561 cgctgaaagt cctgatcttg ttattgttgg tggcggaatc gcaaatgcag atgatccagt
    10621 agaagctgca aaacaatgtc gcgctgcaat cgaaggtaag taatatggct aaatttagtg
    10681 actatcaatt aattctagat gaattaaaga tgactttgtc acatgttgaa gcggatgagt
    10741 tttcaacttt tgcatccaaa atactacatg ctgaacatat atttgtagct ggcaaaggac
    10801 gttcaggatt cgtggcgaat agttttgcaa tgcgcttaaa tcagctcggc aaacaggcac
    10861 atgttgttgg agaatcaacg acacctgcga ttaagtcgaa tgatgtattt gtaattatct
    10921 ctggttcagg ttccacggaa catttaagat tattagcaga caaagcaaaa tcagtaggtg
    10981 ctgacatcgt attaattact acaaataaag attctgcaat aggcaatcta gctgggacga
    11041 acatcgtttt gcctgcaggt acaaaatatg atgaacaagg ctcggcacaa ccattaggaa
    11101 gtttgtttga acaagcatct caattatttt tagatagtgt tgtaatggga ttgatgactg
    11161 aaatgaatgt tacggaacaa acgatgcaac aaaatcatgc taatttagaa taaaataaag
    11221 atagtcgata atatgatgcc taggcagaaa tattatcgat tattttttta tttaaataat
    11281 aaattatagt ataatatcaa taataaacga ataggggtgt taatattgaa gtttgacaat
    11341 tatatttttg attttgatgg tacgttggca gacacgaaaa aatgtggtga agtagcaaca
    11401 caaagtgcat ttaaagcatg tggcttaacg gaaccatcat ctaaagaaat aacgcattat
    11461 atgggaatac ctattgaaga atcattttta aaattagcag accgaccatt agatgaagca
    11521 gcattagcaa agttaatcga tacatttaga catacatatc aatctattga aaaggactat
    11581 atttatgaat ttgcgggtat aactgaagcc attacaagtt tgtataacca agggaaaaaa
    11641 cttttcgtgg tgtctagtaa gaagagtgat gtattagaaa gaaatttatc ggctattgga
    11701 ttaaatcact tgattaccga agctgttgga tccgatcaag taagtgcata taaaccaaat
    11761 cctgaaggca tacacacaat tgtgcaacgc tacaatttaa atagccaaca aacggtgtat
    11821 attggtgatt caacgtttga tgttgagatg gcacaacgtg ctggtatgca atctgcagct
    11881 gtcacttggg gtgcacatga tgcaaggtca ttacttcatt caaatccgga ttttattatt
    11941 aatgatccat cagaaattaa taccgtatta taaaacttgt taaaacagag aataccatgg
    12001 ttaaaatgca tattcataaa tattagatta tacttagaaa tatttcgctt tagattagga
    12061 atttaaaata aatatttatt aaacattatg aatttttaaa gagtaatgtc tgactcgttg
    12121 ataatttatt tttgtaaaaa taaattaaag taatgacaaa gttattgaag taaattgagt
    12181 ataaacattt aaatacgatg tcgaaaatgg cgatagcata tcacttacat gaagttatgt
    12241 gctatcgcta tttttagtta taattccaaa aagttaatcg ttcgatgatt taagaattat
    12301 tattgtttaa ttcaaatgta tgagggtata aaatcattga atttaattcg ataaagcgaa
    12361 atttttgaac aaacatactt ttgtatttat ataaaagttt aaattcttat aaatttgaca
    12421 aaactaatta actccgtata attatgaaac atacaagagg gagtgtatga attcatggat
    12481 tttaataaag agaatattaa catggtggat gcaaagaaag ctaaaaaaac cgttgttgca
    12541 accggtatcg gtaatgcaat ggaatggttc gattttggtg tctatgcata tacaactgcg
    12601 tacattggag cgaacttctt ctctccagta gagaatgcag acattcgaca aatgttgact
    12661 ttcgcagcat tagccattgc gtttttatta agaccaattg gtggtgtcgt atttggtatt
    12721 attggtgaca aatatggacg taaagttgta ttaacatcta caattatttt aatggcattt
    12781 tcaacattaa ccattggatt attgccaagc tatgatcaaa ttggactttg ggcaccaata
    12841 ctattattgc ttgcaagagt actacaaggg ttttcaacag gtggagagta tgcgggggca
    12901 atgacatatg ttgccgaatc atctccagat aagcgtcgta actcattagg tagtggacta
    12961 gaaattggga cattatcagg ttacatagct gcttcaatta tgattgctgt attaacattc
    13021 tttttaacag atgaacaaat ggcatcattt ggttggagaa tcccattctt actcggttta
    13081 ttcctaggat tattcggctt atatttacgt cgtaagctgg aagaatcacc agttttcgaa
    13141 aatgatgttg caacacaacc agaaagagat aacattaact ttttacaaat catcagattt
    13201 tattacaaag atatatttgt atgttttgta gctgttgtat tcttcaatgt tacaaactat
    13261 atggtaactg catatttacc aacctattta gaacaagtta ttaaattaga tgcaacgaca
    13321 acaagtgtat taattacttg tgtcatggca ataatgattc cattagcatt aatgtttggt
    13381 aagttagcgg ataaaatagg tgaaaagaaa gtatttctaa ttggtactgg tgggctaaca
    13441 ttattcagta tcatcgcatt tatgttatta cattcacaat catttgttgt aatagtaatc
    13501 ggtatattta tattaggatt tttcttatca acttacgaag cgacaatgcc agggtcgtta
    13561 ccaacgatgt tttacagtca tataagatat cgaactttat cagtaacatt taatatctct
    13621 gtttcgatat ttggtggtac gacgccatta gttgcaacat ggttagttac gaaaactgga
    13681 gatccattag cacctgcgta ttatttaaca gcaatcagtg ttattggctt tttagttatt
    13741 acattcttac atttaagtac agcaggaaaa tctctaaaag gttcgtatcc aaatgtagat
    13801 aacgagcaag atagagctta ttatgcagaa catccaaaag aagcattatg gtgggttaaa
    13861 gaacgtaaga attagagatt ttaataaaaa gtataaatca atcgtatata agcactttaa
    13921 agctagtagg ttctgctaac tttaaagtgc tttttaaatt gagaactgta attagccgta
    13981 ataaagtttt tgtatataca taaaccccca ctgcaatgat tatcgcaatg ggggaaagag
    14041 gggacttaaa gcatatgttt agctttgaat acttaaaatt ctcttgctat tgaaatgtta
    14101 ggatgtaaat atgtcttaga gtattttgtc caacgcaatt aatattgaga ctctaacctt
    14161 caatattatt atagagaaca caaacttaaa tagattgggt gacttatttg tgtcagttat
    14221 tgcgattgcg ataacttctt ttctctatat acatatagta acgtcttatc taataaaaaa
    14281 catggtacta cagtatcaaa tttatctagg gcttaagttt gatttttata ataggcaggt
    14341 ttacctgata aaaatactta ttcattatat aatgttaaca atatgtattt taaagtttac
    14401 attgagtgag ggatattgat gaacgtaatt ttagaacagt tgaaaacaca tactcaaaat
    14461 aaacctaatg acatagcatt acatatcgat gatgaaacaa ttacatatag tcaactaaat
    14521 gcccgcatca ctagcgcagt tgaatctttg cagaaatatt cacttaaccc tgtcgttgct
    14581 attaatatga aatcaccggt gcaaagtatt atttgttatt tagctttgca tcgtttacat
    14641 aaagtgccta tgatgatgga aggtaaatgg caaagtacta tacatcgtca attgattgaa
    14701 aaatatggta ttaaagatgt aattggagat acaggtctca tgcagaatat agactcaccg
    14761 atgtttattg attcaacgca attacagcac taccccaatt tattacatat tggttttact
    14821 tcagggacaa ctggactgcc aaaagcatat tatcgtgatg aagattcatg gttggcttct
    14881 tttgaagtta atgaaatgtt gatgttaaaa aatgaaaatg caatagcagc ccctggacca
    14941 ctatcgcact cgttaacatt atatgcgtta ttgtttgctt taagttccgg tcgtactttt
    15001 ataggacaga ccacttttca tcctgaaaag ttacttaatc aatgtcataa aatatcatca
    15061 tacaaagttg ctatgtttct tgttccaacg atgattaaat cattattgtt agtttacaac
    15121 aatgaacata caatccaatc attttttagc agtggagata agctgcattc ttctattttt
    15181 aaaaagataa aaaatcaagc aaatgacata aatttgattg aattttttgg tacatcggaa
    15241 accagtttta tcagctataa cttgaatcag caagcaccag ttgaatcagt aggtgtgcta
    15301 tttccaaatg tggaattgaa aacaacgaat cacgatcaca atggtatagg aactatttgt
    15361 ataaaaagta atatgatgtt tagtggctat gtaagtgaac aatgtataaa taatgatgaa
    15421 tggtttgtta ctaatgataa tggctatgta aaagagcagt atttatattt aacgggacgt
    15481 caacaggata tgttaattat tggtggtcaa aatatatatc cagcacatgt tgaacgcctt
    15541 ttaacgcaat cttcgagcat tgatgaagca attatcatcg gtattccaaa tgagcgtttt
    15601 ggtcaaatag gcgtattgct ttattctggt gatgtgacac ttacacataa aaatgtaaaa
    15661 caatttttaa aaaagaaagt gaaacgctat gaaattccat cgatgattca tcatgtagaa
    15721 aagatgtatt acactgcaag tggtaaaatt gctagagaaa aaatgatgtc gatgtatttg
    15781 agaggtgaat tataatatga atcaagcagt catagttgca gctaaacgaa ctgcatttgg
    15841 gaaatatggt ggcactttaa aacatttaga gccagaacaa ttgcttaaac ctttattcca
    15901 acattttaaa gagaagtatc cagaggtaat atctaaaata gatgatgtag ttttaggtaa
    15961 tgttgttggg aatggtggca atattgcaag aaaagcattg cttgaagcgg ggcttaaaga
    16021 ttcaatacct ggcgtcacaa tcgatcggca atgtgggtct ggacttgaaa gtgttcaata
    16081 tgcatgtcgc atgatccaag ccggagctgg caaggtatat attgcaggtg gtgttgaaag
    16141 tacaagtcga gcaccttgga aaatcaaacg accgcattct gtgtacgaaa cagcattacc
    16201 tgagttttat gagcgtgcat catttgcacc tgaaatgagc gacccatcaa tgattcaagg
    16261 tgctgaaaat gtggccaaga tgtatgatgt ttcaagagaa ttacaagatg aatttgctta
    16321 tcgaagtcat caattgacag cggaaaatgt aaaaaatgga aatatttctc aggaaatatt
    16381 acctataacc gttaaaggag aaatattcaa cactgatgaa agtctaaaat cacatattcc
    16441 gaaagataac tttggccgat ttaagcccgt gatcaaaggt gggaccgtta ccgctgcgaa
    16501 tagttgtatg aaaaatgatg gtgcagtttt attgcttatt atggaaaaag atatggcata
    16561 cgaattaggt ttcgagcatg gtttattatt taaagatggt gttacggtag gtgttgattc
    16621 taattttcct ggcattggtc cagtaccagc catttccaac ttactaaaaa gaaatcaatt
    16681 aacgatagaa aatattgaag tcattgaaat taacgaagcg ttcagtgcac aggtagttgc
    16741 ctgccaacaa gctttaaata tttcaaatac gcaattaaat atatggggtg gtgcattagc
    16801 atcaggtcat ccatacggtg caagcggtgc ccaattagtg actcgattat tttatatgtt
    16861 tgacaaagag actatgattg catctatggg gataggggga ggtctaggaa atgcagcatt
    16921 atttactcga ttctaaccag cgattaaatg tgtcattttc taaggatagt gtggctgcat
    16981 attatcagtg ttttaaccaa ccttatagaa aagaagtacc accattaatg tgtgcgtcat
    17041 tatggccaaa atttgattta tttaaaaaat atgcaaatag cgaactgatt ttaacaaaat
    17101 cagcaattaa tcaaactcaa aagatagaag tagacacaat atatgtaggg catttagaag
    17161 atattgaatg ccgacagact cgcaatatca cacgttatac aatggcttta acattaacta
    17221 aaaatgatca acatgtcata acggttacac aaacttttat taaggcgatg aagtagagat
    17281 gaagtttaat gagatatgga taaatgaata tttggcgctc gtaaatgatg ataatccaat
    17341 acataatgag attgtgccag gacaattagt gagtcaaatg atgctgatgg ctatgtcatt
    17401 agagacaaac cagtgtcaaa ttaactacgt taaacctatt ttaataaatg aaaatatcga
    17461 attcattgaa caacacgaac acgaaattat agcaattaat gacgatggag agattaaaat
    17521 aaaaatttct ttgagcacaa aaaaataacc gatattagct gcatgaacgc atattaatta
    17581 ggagatgaaa ggacagctaa tatcagttat gtattgttat tattattggg aacagagatg
    17641 aatataggtt acgtttcttt ctttgcacgg ggatgcatta atctaaaata ataataacaa
    17701 ctatatcaat gtttaataaa ttctggatta ttggaacgat tagtcaattt aactaacttt
    17761 catatgatct atatcgtctt gtaataaaga gagcaatttg aatatttcag tatcactaaa
    17821 tgaatcgtca catttaattg aaacatgctg aaacgttttg gttataattt cataaactgg
    17881 tgcgccttca tggtgatact gtcgataaat aatcataacc tatattacct cctttgctac
    17941 tctatggtta tattataaat aac
//
LOCUS       DANJFZ010000049        17852 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.049, whole genome shotgun sequence.
ACCESSION   DANJFZ010000049 DANJFZ010000000
VERSION     DANJFZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.049"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            147..1217
                     /locus_tag="PWN45_001464"
     CDS             147..1217
                     /locus_tag="PWN45_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDL0623233.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            1413..2174
                     /locus_tag="PWN45_001465"
     CDS             1413..2174
                     /locus_tag="PWN45_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDL0623234.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            2192..2686
                     /locus_tag="PWN45_001466"
     CDS             2192..2686
                     /locus_tag="PWN45_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0623235.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            2700..3926
                     /gene="pepT"
                     /locus_tag="PWN45_001467"
     CDS             2700..3926
                     /gene="pepT"
                     /locus_tag="PWN45_001467"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL0623236.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(4115..5239)
                     /locus_tag="PWN45_001468"
     CDS             complement(4115..5239)
                     /locus_tag="PWN45_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0623237.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(5664..5984)
                     /gene="ytxJ"
                     /locus_tag="PWN45_001469"
     CDS             complement(5664..5984)
                     /gene="ytxJ"
                     /locus_tag="PWN45_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDL0623238.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(6138..7016)
                     /locus_tag="PWN45_001470"
     CDS             complement(6138..7016)
                     /locus_tag="PWN45_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDL0623239.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            7136..7654
                     /locus_tag="PWN45_001471"
     CDS             7136..7654
                     /locus_tag="PWN45_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDL0623240.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            7781..8704
                     /locus_tag="PWN45_001472"
     CDS             7781..8704
                     /locus_tag="PWN45_001472"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL0623241.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            8722..9036
                     /locus_tag="PWN45_001473"
     CDS             8722..9036
                     /locus_tag="PWN45_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623242.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9353..10381)
                     /locus_tag="PWN45_001474"
     CDS             complement(9353..10381)
                     /locus_tag="PWN45_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0623243.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(10500..11261)
                     /locus_tag="PWN45_001475"
     CDS             complement(10500..11261)
                     /locus_tag="PWN45_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623244.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(11258..12214)
                     /locus_tag="PWN45_001476"
     CDS             complement(11258..12214)
                     /locus_tag="PWN45_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0623245.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(12201..13172)
                     /locus_tag="PWN45_001477"
     CDS             complement(12201..13172)
                     /locus_tag="PWN45_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623246.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(13211..13366)
                     /locus_tag="PWN45_001478"
     CDS             complement(13211..13366)
                     /locus_tag="PWN45_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623247.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(13880..14851)
                     /gene="nrdF"
                     /locus_tag="PWN45_001479"
     CDS             complement(13880..14851)
                     /gene="nrdF"
                     /locus_tag="PWN45_001479"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL0623248.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(14969..17074)
                     /gene="nrdE"
                     /locus_tag="PWN45_001480"
     CDS             complement(14969..17074)
                     /gene="nrdE"
                     /locus_tag="PWN45_001480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL0623249.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(17037..17435)
                     /gene="nrdI"
                     /locus_tag="PWN45_001481"
     CDS             complement(17037..17435)
                     /gene="nrdI"
                     /locus_tag="PWN45_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0623250.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
BASE COUNT         5842 a         3108 c         2573 g         6329 t
ORIGIN      
        1 tattattcat tttttcatca tatcatttaa ggcaacattt taacatcaaa gtctatatca
       61 tacattaatt ttatatgtaa actaatgaca aattgtaagt tttcacttac aatagctagt
      121 ggtcaattaa aggagtcgat tcagacatgt tcgaagcatt tatatacaat atatctgtta
      181 tagtcgctgg aatctattta ttccatcgct tacagtattc agaaaacaaa cgtatggttt
      241 tttctaaggc ttatgtcact gttttaatga caatcgtatc attattatta tctgtctacc
      301 ctatccctta tcgtgaagat tatttaattc acttaacatt cgttccttta ttgtttttag
      361 gtagatttac aaatatggtc tatacattat ctgccacagt aattgtagca attgttgaaa
      421 ttgtagtatt taacaactcg attatgtacg gtgttacgtt gattgttatt gctgcagtaa
      481 caagtgctat tggaccattt ttaaaacaaa atgatgtgct atcattatta atacttaatg
      541 ttgttacaat aatcatttta tttggtgtcg cgttagtgag tcctatttac acactatcgg
      601 aagttatcat tttaatacca atttcattaa ttatcacttt agcttcagcg ataacatttg
      661 tcgatatttg gcatttcttc tcactcgtaa atcgttatga aaacgaagat aaatatgatt
      721 acttaacagg cttaggtaat gtaaaagaat ttgatagaca tttaaatgaa atttcacgaa
      781 aagctgaaaa agaacatcaa agtatcgcat tattattaat cgatatcgat ggatttaaag
      841 atgtcaatga tacctactca cacaaatcag gtgatgctgt attaaaacaa atgtctcaat
      901 tacttaaaaa ctatgtgccg aatcaattta aaatttttag aaatggtggc gaagagttct
      961 cagttgtcat tcacaattat tcacttgatc aaagtgttaa attagctgaa aatattcgtt
     1021 cgggtgttga aaaatcatca ttccatttac caaacaaaga agttattaaa ttgtcagtgt
     1081 ctatcggtgt tggttattta acagatgacg atccaaaatc acagcgtaaa gtgtttaaag
     1141 atgcagacga tatggtgcat gtagccaaaa atcaagggcg aaacaaagta atgtttaacc
     1201 ctattatcaa tttataacaa cgacaaaact gttaatcctt tgctattatt aaatcatagt
     1261 ataatagcaa agattaacag tttttcttat ttaaatttga accatacaag ttcatcatac
     1321 taacacaaag tctcactatt gaaagttgta acaacaagtt gtaaacttca attgttcaaa
     1381 taaataacac atatcattgg aggtgaattg atatgccaga ttcaatcaca attatagatg
     1441 aaaacaaagt gattgatgtt gtattaattg caggtagaat tttacttgaa tcaggtgctg
     1501 aaacatatcg agttgaagat acaatgaacc gtatcgcaca tagttatggt cttcataata
     1561 catatagttt tgtcagttca actgcaatta ttttttcatt aaacgatcga acaagtacaa
     1621 gattaattcg tgtacaagag cgtacaacag atttagagaa aatcgcttta acgaatagtc
     1681 tttcacgtaa aatatcaaat aaagaactca caattgatga agctaaatct gaatttattc
     1741 atttacagca tgcatcatta caatattctt ttttaacgaa tttctttgca gctgccattg
     1801 catgtggctt cttcctattt atgtttggtg gtgtcgcatc agattgttgg attgcagtca
     1861 ttgctggcgg atccgcattt ttaacattca gctttgtgca acgctatata caaattaaat
     1921 ttttctcaga gtttgttgca gccgctgtgg tcatatccat tgcagccaca tttactaaat
     1981 taggcattgc aaccaatcaa gatatcatta ctatagccag tgtcatgcca cttgtccctg
     2041 gtatattaat taccaatgcc attcgagatt tattagcggg tgaattactt gcaggtatgt
     2101 ctcgcggtgt tgaagctgca ttaacagcat tcgcaatcgg tgctggtgtc gcaatcgttt
     2161 tattaatcat ttaaggaagg actgttaaag catgctattt tatttatttc attttacaat
     2221 cagctttata tcaacagtac ttttctctat cattttcaat gcacccaaac gcctcttagt
     2281 agcatgtgga tttgtgggtg ccattgcatg gacgatttac caattaacgg tagatttaga
     2341 gtttggaaaa gttggcgctt catttttggg aagcttaatt ttaggcttaa tgagtcatac
     2401 tatgagtcgc agatataaac gaccggtaat tatattcata gtgccaggca ttataccatt
     2461 agtacctggt ggtgcagctt atcaagcgac tcgtttttta gtatcaaatg attatacaag
     2521 tgctgtaaat acatttttag aagttacact gatttcaggt gcgattgctt tcggtatatt
     2581 agtttctgaa attctatatt acctatacac acgtatcaaa caactgtatg gtaaaatcaa
     2641 aggtaagaca tataaaaaat cttacaacat gaataataga gtttaagggg tcgaatataa
     2701 tgaagaacca attgatagat agattaacaa gatatacgac aattgatacg caatctgatc
     2761 caaaatctac aacaacacct tcaactgaaa agcaatggga tttgttacat ttattagaaa
     2821 aagaattaca acaattagga ttgccaactg atttagatga aaatggctac ttatttgcta
     2881 cattagaaag taacatcgat gtcgacgtcc caacagttgg atttttggcg catgttgata
     2941 catcacctga tttcaatgct tcaaatgtta aaccgcaaat tattgaaaac tatgatggta
     3001 agccatacaa actaggcaat actaaacgtg tcttagatcc taaagtattt ccagaactta
     3061 acagtttagt tggtcataca ttaatggtaa ctgatggtac atcgttacta ggtgcagacg
     3121 ataaagcggg tattgtggaa attatggaag ctatttgtta tttacaagaa catccagaaa
     3181 ttaaacatgg taccattcgc attggattta caccagacga agaaatcggt cgtggtccac
     3241 ataaatttga tgttgaccgc ttcaatgctg attttgctta tactatggat ggtagtcaat
     3301 atggagaatt acaatatgaa agctttaacg ctgctgaagc ggttattaca tgccacggtg
     3361 taaatgttca tcctggttca gctaaaaatg caatggtaaa cgcaatacgt ttaggtgaac
     3421 aattcgatag tttgctacct gatagtgaag ttccggagcg aacagaagga tacgaaggct
     3481 tttatcactt aatgaacttt gaaggaactg ttgaaaaagc aactttgcaa tacattattc
     3541 gtgatcatga taaaaaacaa ttcgaattgc gtaagaaacg tattttagaa atacgtgacg
     3601 atatcaatgc ccattttgaa aattatccag ttaaagttga tatatcggat caatatttca
     3661 atatggcaga aaaaatatta ccattgcctc atattattga tatacctaaa cgtgtctttg
     3721 ccaaattaga tattccagca aacactgaac ctattcgcgg tggtacagat ggttcacaat
     3781 tgtcatttat ggggttacca actccaaaca tctttacagg ttgcggtaac ttccacggcc
     3841 catatgaata tgcatccatt gatgttatgg aaaaagcagt acaagtaatc attggaattg
     3901 tagaagatat cgctgaaaat cactaaaaac caacgattat atactagttc aactcaggct
     3961 taattaatat ataaaaatca cgctaacatt cagtgcaatg atagcgtgat ttttcatatt
     4021 ttttacgtat actttcgatc ttctaattcg ttttgctaac catcataaat gttattaacc
     4081 atcatgacag catcaccaat ttagtccttc tttactaaac ctcaacatta aaatgtgttt
     4141 tcaacagttt taaagactga accgtataat gcctaatttg taaccccatt ttaaagtcag
     4201 tataagtttc tggcatatcg ataaatgtat taaacattgc tgtaacccct tgtgattcaa
     4261 ataaatcaaa cttttcagca gttgcacaaa ttgcaatggc aaccttttga tgtttatgac
     4321 aaagttctgc aatacgcaat gttgtcgttt ctagcaactg atcattttca tttaatcctt
     4381 ctccaaaaat aattaaatcc gcttgttcaa ctaaattttc taaatgtgtt agttggtcta
     4441 ctaatgcatg actggttaat atttcagctt gatacagtcc attcaagact gctgcaattc
     4501 caccaccagc accaccacgt tcaactggac caattgcaat tttcaattca cttttaaata
     4561 actcactaaa ataccaaatt aaattatcga tttctgctgc ttgattatga ttcaactgat
     4621 acgcatcata agtttgcatg atttcacttt gcttaccata taatcgactt gaaaaatccg
     4681 acattacttg aattcttgct gtttccattt tagggtgtaa gttcgacata tccatacgac
     4741 gaatatattt aattacacca gcaccttgtc tcatatctac gacacgccct tcgtcatcat
     4801 agaattgagc acctaatgct tgtaacatac ctgcaccagc atcaaaacta tcaatcccac
     4861 ctagtgaaat tacaacatgt tttgcgtcgt tatctaatgc atgtttaatc atttctccta
     4921 aaccataact tgttcgttca acaattggtt ttttaccttt taaaaataaa ttcccctcga
     4981 taacggtcat ccctgtatca gtttgtccgt aaacaccttc aacttcattc atatctgcat
     5041 catgtactgg tatacgatac ttttgcccag attgccataa aaatacagaa tctaataatt
     5101 catgtcttcc attaaacaat ggtacttgaa ctacatctgc agtttcaatt tggcttgcaa
     5161 ctgcctcttc aacatatcta ttagcttgat aacttgaaat aattccatga aactcatcca
     5221 tggctactaa tactttcata accttgacca ccttacttta ttatttgttg tgatacgttt
     5281 tttattcata aaagattaat cttaaatttt agattttatc tcgtgattat atgcgaacat
     5341 ttaacatgac atgattatta attattccag taaataacaa tatgcttctt ttggtttagc
     5401 atatatttcc tattaaacta acttacaaat catgtttaaa ttttcttatt ggattcgtcg
     5461 ttttccaaaa tattttcaat aaaaatactc gaatatatta tacatatgtt tgtaaaaaag
     5521 tactaactta actgcttcta aaggtgacat aaaataaaag gtcagaacat cattatagcc
     5581 aaactactct gattcacacg aatttaagtt gtgctattca cgtctagtag tgtaaggcaa
     5641 aatgttccaa ccctatagtt tcattattct tctgcttgtg ctaatgacga cacattgata
     5701 tcaccgtggt ctcgattcca aaccatttca ccatttacaa aataaaatgc ttgaggtgat
     5761 tcatgtttaa cgttcgtttt tttagcaata taatctgaca aatcgcgttc ttgttggaca
     5821 atcaaataat aaccgtccat atcgcgttca tataaaaatt tattaaattg atcgtacgca
     5881 tttgccgata ttggacaagt ttcactatgt tttaatacaa aaacatattt attttcctca
     5941 ataacctgtt caaattggtc aattgaactt agctttatag ccactctatt cacctctaaa
     6001 aatatttatc acatttaaat agcatctgcg tgacattttt atacatatag ttaatatcaa
     6061 tgtttaccat tatacacata gtaagttgac atgttaacca taaattcaaa atttttaact
     6121 ttgaaaaatg attatagtta tttttcttct aatttttcaa gctttttttc aaaggcttta
     6181 tcgccgtgag ttatatctat accctttttc ggcaacttat ctacatcgac ttttgataac
     6241 ttctcactaa cctttattgt ttcaatacgt gtattataat agttctgcag actgtaatac
     6301 atttctattg cgtttttcct tgcattatta acatgcttat cactaatatt agtttggtta
     6361 atatcggtaa tttgcttttc aatcattggc ataatgtgat ttttaatcgc gccttttact
     6421 tcattttctt tactatcatc taggttcttc ttcgcagtat ctcttaacgc tttattatta
     6481 tgttctaatt ttgtcgtaag attagttgct tcacttttat tatcagctaa tttaatttct
     6541 gattcaactt tgtatctatt tttttcaaat tgtttcgtat aatctaaaat gtcttcgtta
     6601 tacttgatag attgattaca taaacctatg aattttttta attgatatat cgcatccttc
     6661 ttctcatttg caagcgtcat atattctttt tttaatttct taactttcat tgtatcatca
     6721 ggcaaatttt ttgctgactt ataatatgct tcaaatttag gtatgagatg gtttttaaca
     6781 tcttcttgta gtgccttaaa ttctttacta tttttatcag ttgtatcagt tttacttaga
     6841 tgatcaattt ctttcaaatg tatattatcc atgacatgtt gtaattcttt ttgtttttgc
     6901 tcaacatctt taaattgctt agtaaattga tgttgtgcct ctttttcttt attaccacaa
     6961 gcacttatta ctacaaataa aatcgctaaa acagcgataa taacaatttt tttcatgatt
     7021 gcactcctta acagattcat tatacaaaaa catgtttatt atgaaaatga attatgttat
     7081 agtattccag actttatcgt gattaaatta attttcaata tcaaaggagt ttatcatgta
     7141 ttcaaatgtt caaccattca ttaatagtga tttaactaat tgttttcaat cacaatacac
     7201 tgaaattgaa tcattattat tcaacctctt agacagccca gtaaaatata caaaacatat
     7261 tggcggtacg catcatttta aatattcaac agagccaatt ttagatattt tagtaggtgt
     7321 agaaaatttg catgatatta catccttaga tgaaaaacgc ttaaattatg tagggtttta
     7381 tcgtttgcat cataaatata ctaagaaagt tatgatggcg aagtttaaca attttcatga
     7441 actaaaacaa caggtgcgtc tacatatcat tcaaatgaat acccaaacat ttgaatctta
     7501 tcttgcactt gattacctct tatcaactaa tgatgatatt gctttgcaat tcaatacaaa
     7561 aaaacaccaa atattgcaac atagcttaac aattagacaa tatgagatgc aaaagcaaca
     7621 atactttgaa aaactatgta aaaaatactt ataacgacaa taatgccgta ttttataatt
     7681 atactaattc taatactttc ttttcaattt tcgcaaatga attttaaaat tggtataata
     7741 ctatatgata ttaaagacat gagaaaggat gtactgagaa gtgataaata aagacatcta
     7801 tcaagcttta caacaactta tcccaaatga aaaaattaaa gttgatgaac ctttaaaacg
     7861 atacacttat actaaaacag gtggtaatgc cgacttttac attaccccta ctaaaaatga
     7921 agaagtacaa gcagttgtta aatatgccta tcaaaatgag attcctgtta catatttagg
     7981 aaatggctca aatattatta tccgtgaagg tggtattcgc ggtattgtaa ttagtttatt
     8041 atcactagat catatcgaag tatctgatga tgcgataata gccggtagcg gcgctgcaat
     8101 tattgatgtc tcacgtgttg ctcgtgatta cgcacttact ggccttgaat ttgcatgtgg
     8161 tattccaggt tcaattggtg gtgcagtgta tatgaatgct ggcgcttatg gtggcgaagt
     8221 taaagattgt atagactatg cgctttgcgt aaacgaacaa ggctcgttaa ttaaacttac
     8281 aacaaaagaa ttagagttag attatcgtaa tagcattatt caaaaagaac acttagttgt
     8341 attagaagct gcatttactt tagctcctgg taaaatgact gaaatacaag ctaaaatgga
     8401 tgatttaaca gaacgtagag aatctaaaca acctttagag tatccttcat gtggtagtgt
     8461 attccaaaga ccgcctggtc attttgcagg taaattgata caagattcta atttgcaagg
     8521 tcaccgtatt ggcggcgttg aagtttcaac caaacacgct ggttttatgg taaatgtaga
     8581 caatggaact gctacagatt atgaaaacct tattcattat gtacaaaaga ccgtcaaaga
     8641 aaaatttggc attgaattaa atcgtgaagt tcgcattatt ggtgaacatc caaaggaatc
     8701 gtaagttaag gagctttgtc tatgcctaaa gtttatggtt cattaatcga tactgaaaca
     8761 cgctgtcgcc attattttac cgaagaagat attattgcta ttaaatttaa atgttgtaat
     8821 aaatactatc catgctataa gtgccataat gagtttgaaa agcacgccat taagcgttgg
     8881 tctgagcctt catttaacga aaaagcaatc ttatgcggtg tatgcaaaca cgaattaaca
     8941 atcaatgaat atatgatggt agagcgttgt ccaaattgcc aatctcggtt taacaatcgc
     9001 tgtaaatatc actatcatat ttattttgaa atttaaacaa catagtcaaa acattatagg
     9061 gagtgggtca gacatgataa agcaccactt ctgtagaatt cctttttgaa attctctatg
     9121 tttgggcccc gccaacttgc attgtcagta gaaattgaaa atcaaatttc tctatgtttg
     9181 gagcccacta gtattgaaat gaacttgtta caagtacatg ttcattcaac caacgactga
     9241 caacagaaca ttttagagtc taggacattg attgacgtcc cagatttcct tttttaaaga
     9301 aaagagggga gcaatgatta agacctttaa ccattgttcc cctcttttaa aattatttta
     9361 caactttatc aagttcctca atttgtttaa ttgtagttgt agttgatcca gctgcaaagt
     9421 accataattt aggatctaaa ttatatactt tgtcttcttt aattgcttta acatttttta
     9481 atacaggatt atttaatgct tgtttcgcag ttgatttacc acttatcgct tgacctctat
     9541 ccatcgctaa aataacatct ggattttctt tattaacata ttcgttagaa acattttgtc
     9601 catgattgct attacttact tttttatcaa ctgcattgaa tcctaatgta tcgtaaacta
     9661 atccaccaaa acgaccttta ggtccaaatg ttgataattc accttcgtta actagtaaat
     9721 acataacagt tttattgaag tttttcgttt tatctttcat tgaagcaatt ttgttatcta
     9781 aatctttatt taattcttta gctttatctt ctttatcgta aatttttccg atattttcag
     9841 tgttttgttt cattgaacca attaagttct tttcatctgc accaacataa acaattttcg
     9901 ctttaggtgc agcttttttg aattcatcta aattcttttg attagctgta cgtccagaga
     9961 taaagattac ttcgggtttc gtcgcagcaa ttttatcaaa attcacttct tttaaattac
    10021 caacgtttgt atatttatca tctttaaatg attctaagaa attcggtaat gacttaccgc
    10081 cttccccttt aggtaatgct tttactttat ctgataagcc catttctttc attacatcta
    10141 atgcgccata gtctaacaca actgcatttt taggattttt tggtacttca acagtttctt
    10201 taactttttt agcgtcacta ccatcttttt tctcgccacg cattttatag ttattttcaa
    10261 tttttactgt atctttagaa cctttagttt ctgattttga ttgttcttta tcagaattgt
    10321 taccgcatgc cgctaataaa aacattactg ctaatactaa atataagact gttttcttca
    10381 taatataata ttctcctctt ttggcttaat ctgtgtgctt gatgtgaaat ataaataaat
    10441 gctttacgtt taacccttct ttaattggcg catcacccct ttaaagtgtc tccaaattat
    10501 caatccttaa aatataaaca aatcctttga tttcttatat gttcaatttg aatattcatg
    10561 tcatataaat cacttaaaac actactttga atgacattat ctttcaaatc tgacttaact
    10621 agttgtccgt ttttcaatgc tacaatctga tctgaataac aggacgcaaa gttaatatca
    10681 tgtaacacaa tgacaatcgc tttattcatt ttatgcgcta acatttttaa cgtttgcata
    10741 atttgaacag catgcttcat atctaaatta tttaatggtt catctagcaa aatatattca
    10801 gtatcttgtg ctattgtcat tgcaatgtat gcacgctgac gttgtccacc agataatgac
    10861 ttaatattac gatttctgat atcttgtagt tgcaacaaat ctagcgcatc attgacaata
    10921 tcatgatcct ctttcgtcaa acgaccttta gaataaggga atcgtccaaa gtttaccaac
    10981 tgctctaccg taatattcat ttcagtatgg tttgtttgtt ttaatataga tatttttttc
    11041 gacaagtcat tatttttata atcagacatg agctgtccat ctattttcac ttcaccgttc
    11101 tcaaaacgaa ttaacctaca tatcgctgaa agtaaagtac tcttacccgc accattaggt
    11161 ccaattaaag aagtcaattt acctttttcg atatctatgc taatatcttc caatatcatt
    11221 tgattattta tagttttagt taaattttca acttgaatca ttgcgcattt ctccttctaa
    11281 ctaatagata aatgaaataa cttccaccaa tcaaatcaat aagtatactc atttctgtcg
    11341 tagcttcaaa cacattttca actacccatt gcgcactaaa taaactaatc caactcaagc
    11401 aaattgtcgc aattaaaata tacttatgtt catacgtctt cattagttca tgcgctagat
    11461 ttacagttaa taaacctaaa aatgtaattg gtcctactaa tgcagttgac acagaaacta
    11521 aaactgagac tattacaagt agaattcgcg ttaatttttc atacgatatc ccaagattaa
    11581 ttgcttcagc acgacctaaa agcaatacat ctaaataagg caatagtaaa attgtaatga
    11641 ctaataagat tactaatagc actgctgaga aagtaactaa attcgaatta gaagcattaa
    11701 aattagcaaa catactactt tgtattgcta aaaatgactc aggatccata atcagttgaa
    11761 taaaacctgt tatgcttcta aaaaatgttc ctaaaaggac accaataagt aagataaaat
    11821 aaattgaaaa atgtccgatt ttaaagatac cttggaatag gattagtgcg aataacacca
    11881 tcgttataag tgttaatata aagttcaaat atacattagt aacccatacc gattgaattc
    11941 caaataaaaa gactggcaat actttgataa ataaataaac tgcatctaac cccattattg
    12001 atggtgtcaa tagacggtta tttgtaatcg cttgaaaaat caccactgaa gttgcaatgg
    12061 cagcacctac taaaataatt aatatgaact ttcttaaacg acttgaaaat tgatattcaa
    12121 atatttcaaa atcaatacct acaaataagt acagcatact aattaataac gttactgcaa
    12181 ttaaaagcat tattttttta ttattgttgc gcataatttt tcctaccttt cataagcaag
    12241 ataaggaaaa tgattgttcc aaatacacct attgttaaac caatatttat ttcatatgga
    12301 taaacaacaa ttctgccaac tatatcagaa aataatacaa agatggcacc taacatcatc
    12361 gtatgaggga tagcattttt caaatgatca cctcgataaa ttgaaataat atttggtatt
    12421 actagtccta agaacggtaa tgttccaaca gtcaccacta ccaatgctgt aatagttgca
    12481 gtaataaaca atgcgatgtt aattaatttt tcgtaactca aacctaaatt attagtaaag
    12541 tctttaccca ttcctacaat cgtgaaatga ttagcaaaaa gatatgtcaa tgctaaaaga
    12601 ggaatactta aatataaaat ttcatagcgt ccacttgtga taatggcaaa gttcccgtta
    12661 agccagttac caatgctttg aacagcattc gtgcgcaatg agatgaaggt tgtgaaactt
    12721 gcaacaatcc cacccatcat aattcctaaa agcggtacaa aaatgacatc tttcactttt
    12781 ataaaatcaa tgattttaac aaataaaaac gtaccgcaaa tactgcaaat aacagcaaat
    12841 actagtttta ataaaatatg accggttgga aagaacaata aagcaattaa aatacctagt
    12901 ttagcccatt ccatcgttcc agctgtagtc ggactaacaa acttattttg catcatttgt
    12961 tgcattatca agcctgctaa agccaacgaa cttccagcaa ttaaaatact catcgtcctt
    13021 ggtattcggc ttgagaataa aatattccgc tgtgactcag ttaaatgtag taaatcttta
    13081 attgatagtt cactcacacc tatgaacaac gaaataattg tcaacaatat tagtaataga
    13141 agcaaaatat ttccctttaa taaatacttc attacaaatt tttctccctt taaattaatg
    13201 ataatcatta tcattgataa tatttctcat taatagtagc gttttttgac cataatttca
    13261 atagcctttc aaataattta gtgtcaattt tattgatatt tgcaattaaa ctgaaattta
    13321 atttttcgga atgtgtattt tgtttactta aagtaaaata gaacacttaa gattttgatg
    13381 tctgggaata gtggaaatga taaaaactac taatgattga ttaagtagtg gttcttaaca
    13441 ttagcctcag ctaatgtgta cttaaaaata ggaatacatg agtaaaactc atgtggaagt
    13501 gataatttct aaaggaaaaa gtttttcttt atgttggggc cccgcccgaa cttgcacaat
    13561 attgttagct gactttccgc ctgcttttat gttggggccc cgccaacttg cacaatattg
    13621 ttagctgact ttccgcctgc ttctatgttg gggccccgcc aacttgcaca atattgttag
    13681 ctgactttcc gcctgcttct gtgttggggc cccgccaact actgccaata taaatagcat
    13741 tgtacatctt agagcattga tttcttactc agactcataa tgagttagat acacataaaa
    13801 aaagaccttc aacatgtagg agacaagacg aaacgaatcg ctatttccct ttacatgtga
    13861 aggtctttta atttaattgt tatttgttgt caaatacaaa gtcatcatct tgtaatgctt
    13921 ctacgtttaa tgctaataca taaccatcac cttttactga gaagaagtca tggtttttag
    13981 ttgttgtatc taaggcattc tcaatgattg ggttaaattc acgttcctca aaataaggtt
    14041 caaagcctaa gtttgaaagt gctttgtttc cattatattt aacatagttt agcacatctt
    14101 cagtgattcc aagatcatcg tataacattt ttgtgtatga ctcttcattt aaatacaagt
    14161 catttagcaa tttatacatt tcttgatctg ctttttgttt ctcactttca gatagttcat
    14221 ttcgtaaatg ctgtgcatct aaaccggtaa atacaccatg aatagattca tctaaaagaa
    14281 ttttacgaat gatttcacct gatgtcgtca ttttcccttg accagcaaga tatagtggat
    14341 aatagaaacc tgagaagaat aagaatgttt ctaaaaatac actcgtaact ctggccatat
    14401 attggtcgta tatcgaagct tctttacccc aaagtttgtg ataattagca acaattttat
    14461 cagatttata ttttaaatgg ggttcctcta aaacccattc atctaatagg tagtttgttt
    14521 cactagatgg taatagtgtt gtgaaaatat gtgaatagct tttcgcgtgt atttgctcca
    14581 tcatcgccat aaatgaataa actgcttttt tccttaagtc agtcgtatgt agcataacta
    14641 aaggcatgcc atcatctgct tgatgtgtat ctaagcctgt taaaccagct aatgcttttt
    14701 taaatgtgtc ttgttcagct tcagataaag tcttccaact tgcaatgtct tttgatactt
    14761 taaattctgt ttcaacccac atttgagata tattttgtct ccaaaacata ttcgtcatat
    14821 cttcttgtgt gttccaatta acagctatca tgaatataat ttcctcctgt aatgttactc
    14881 aataattgac aaatacagac atagcagtct aatcatctgc taattcagag cagatgatta
    14941 gctgtttgtc attttcaaca tttaattgtt agatagaaca acttgtacat tcttctacac
    15001 ttaataattt atttctagta tagtaaagtg attttaatcc tttatagtgc gcatatacat
    15061 ataatcttgc taactcacgt gtagaaattt cagaattaac ataaaggatc gttgagatac
    15121 cttggtcaat atgcgtttga attgtcgcaa ttaaatcgat taatttcatc tgatcagtat
    15181 tgaatgctga tttgtagtac cacattgttt gtggtgataa gaatggcata gggtaaaatg
    15241 tttccgcatt accataagta cgacgttcaa tttggtcaac gattggcatt acagaacttg
    15301 ttgcattttg aacataagaa atactttgtg ttggagcaat tgctaatcta tatgcatgat
    15361 ataaaccata ttgttcaaca tcttgttgta gtttcttcca atcctcagaa gtaggaatag
    15421 ccataccatc gaataattca cgtacttttt cgaattgagg ttcaaattct tgagttgtat
    15481 agaactcgaa atatttgcca ttagcataat cagacttttc aaagtcttga tatttgatac
    15541 cacgctcttt agcgatttcc attgaacgtt cgattgagta gaaattcatc atcataaaga
    15601 agatatttgc aaaatctttt gcttcttctg actcataacc aattttattt tttgctaggt
    15661 aaccgtgtaa attcatcaca ccaagaccaa ctgaatgtaa ttcactgtta gcttttctaa
    15721 ctcctggtgc attttgaata tttgctacat cactcacaac agttaatgcg tccataccag
    15781 agtgaactga atctctgaat tttccgcttt ccattacatt aacaatattt aatgagccca
    15841 agttacaaga aatatcacgt ttaatttcgt cttcaatacc atagtcatta ataattgaag
    15901 tttcttgtag ttggaaaatt tccgtacata agttactcat tttaatttgt ccaatgtttg
    15961 aattcggatg cactctgtta gcattatctt taaacattaa atatggataa cctgattgta
    16021 attgtgtttg cgcaattaaa ttcaacattt cacgcgcatt ctttttcttt ttctcaacat
    16081 ttgggtttgc aaccatgtca tcataatatt tttctaaatc gatatcgtct aatgtcacac
    16141 catattcttc tttaactgta tgaggtgcaa acatataaaa gtccttacct tctttagcta
    16201 aatcgaagaa tttagatgga acaattaaac cagttgatat tgtagataaa cgtaaatctt
    16261 catccgcatt tactttttta gtatctaaaa attcttctac atcataatgg aagatattta
    16321 agtacacagc accagcacca gggcgttgac caagttgatc tgcatagcta aagccacctt
    16381 caagtgactt agcaataggt aaaacgcctt tcgctacgcc tttaattcct ttaattgctt
    16441 caccacgtgc acgcaattta gataagttaa ttgcaacgcc gcccccaatt ttacttaatt
    16501 gttttgcagt tgaatcaata aagttaattg aatttaagct gtcatccact tctaataaga
    16561 aacatgacac tagctcacca cgacgcgcac ggcctgcgtt taaaaatgtt ggtgtcgctg
    16621 gttgatatct ttgttcaacc atagcagaaa taaattgttt agcttgtgct ttattaccat
    16681 ttgctaggta taaagcaaca atggcaacgt gttgattata gtcttctaag tattgacttt
    16741 tatcatttgt tttcaaagcg taatctttga aaaatttact agctgacata taacttgcaa
    16801 aattaaacgg gattgatttt gcataatcag tgatttcaat tagatccgct tcactataaa
    16861 tatcaaacac attgaaataa aaatcgttgt ctactaaata acgtaaacgc tcgatttcag
    16921 tgtcgaagaa gattgttttg tcttttactt cttctaaata agctactaaa gcttcttggt
    16981 ctttttctaa actaaagaat ccatcttctc ttcgtttagt gacctcatta tttaattcaa
    17041 tatgattgta ctttttctct tccatggttt tcattaaaat tacccacctt gttcttaaat
    17101 tcaataacgt ctttgttttt tccatgtaac tcaaacttca ttaataaagg gacattatac
    17161 tcttctgata tcgtgcgacc cgcttttgcg aaatttagtc cccaatttcg attaccgcta
    17221 gctgccacac ctctgatgta ttgatgatta acttctaaaa aagattgaac gggttctggt
    17281 acttctccaa atccaatagt gccagtaacg ataataaacg gttcatgaac tggttccata
    17341 caattttctg ctgtaatctc aagcgtattt tcaagttctg ttctcttaat aaaacgacgg
    17401 acatttccag taaatgaaaa atatattatt ttcattggat caccttcttt cttcaaccct
    17461 tttaaataaa gtagtcattc caattaaaaa atgctttaac aacatggtta ctatggttat
    17521 ctcagcattg tattaactat aatgaagcta cttattttct caaatgtaaa ccaattgcat
    17581 agatttatat ttgagaaaat agtagcttat atgggttgaa tgtataaaaa tcactatatg
    17641 tagtgtttac atcattggat atcttccatt tgctcagtac attttctaaa aatatgacta
    17701 ttaattgata ataactatta aatttctgtg ttaaattact tttaaatgta atttcttcat
    17761 aaataaagca tttgcataca aaacacaaga tattgtgtct ttttcgtttc accaacacta
    17821 tattatgtgt ttcattatac ataagacaca ct
//
LOCUS       DANJFZ010000050        17506 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.050, whole genome shotgun sequence.
ACCESSION   DANJFZ010000050 DANJFZ010000000
VERSION     DANJFZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.050"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            14..592
                     /gene="pgsA"
                     /locus_tag="PWN45_001482"
     CDS             14..592
                     /gene="pgsA"
                     /locus_tag="PWN45_001482"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL0623251.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            819..1970
                     /locus_tag="PWN45_001483"
     CDS             819..1970
                     /locus_tag="PWN45_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDL0623252.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            2135..3178
                     /gene="recA"
                     /locus_tag="PWN45_001484"
     CDS             2135..3178
                     /gene="recA"
                     /locus_tag="PWN45_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL0623253.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            3532..5091
                     /gene="rny"
                     /locus_tag="PWN45_001485"
     CDS             3532..5091
                     /gene="rny"
                     /locus_tag="PWN45_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL0623254.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(5388..5603)
                     /locus_tag="PWN45_001486"
     CDS             complement(5388..5603)
                     /locus_tag="PWN45_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623255.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            5777..6574
                     /locus_tag="PWN45_001487"
     CDS             5777..6574
                     /locus_tag="PWN45_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL0623256.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            6714..8474
                     /locus_tag="PWN45_001488"
     CDS             6714..8474
                     /locus_tag="PWN45_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDL0623257.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            8475..9341
                     /locus_tag="PWN45_001489"
     CDS             8475..9341
                     /locus_tag="PWN45_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDL0623258.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            9435..9728
                     /locus_tag="PWN45_001490"
     CDS             9435..9728
                     /locus_tag="PWN45_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDL0623259.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            9862..11406
                     /gene="miaB"
                     /locus_tag="PWN45_001491"
     CDS             9862..11406
                     /gene="miaB"
                     /locus_tag="PWN45_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL0623260.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            11407..11772
                     /locus_tag="PWN45_001492"
     CDS             11407..11772
                     /locus_tag="PWN45_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDL0623261.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            11799..12290
                     /gene="thiW"
                     /locus_tag="PWN45_001493"
     CDS             11799..12290
                     /gene="thiW"
                     /locus_tag="PWN45_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDL0623262.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            12593..15211
                     /gene="mutS"
                     /locus_tag="PWN45_001494"
     CDS             12593..15211
                     /gene="mutS"
                     /locus_tag="PWN45_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0623263.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            15224..17233
                     /gene="mutL"
                     /locus_tag="PWN45_001495"
     CDS             15224..17233
                     /gene="mutL"
                     /locus_tag="PWN45_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL0623264.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            17239..>17506
                     /locus_tag="PWN45_001496"
     CDS             17239..>17506
                     /locus_tag="PWN45_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDL0623265.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKV"
BASE COUNT         6567 a         2346 c         3291 g         5302 t
ORIGIN      
        1 atgaggatgt ataatgaata ttccgaacca gattacggtt tttagagtag tgttaatacc
       61 agtttttata ttgtttgcgt tagttgattt tggatttggc aatgtgtcat ttctaggagg
      121 atatgaaata agaattgagt tattaatcag tggttttatt tttatattgg cttcccttag
      181 cgattttgtt gatggttatt tagctagaaa atggaattta gttacaaata tggggaaatt
      241 tttggatcca ttagcggata aattattagt tgcaagtgct ttaattgtac ttgtgcaact
      301 aggactaaca aattctgtag tagcaatcat tattattgcc agagaatttg ccgtaactgg
      361 tttacgttta ctacaaattg aacaaggatt cgtaagtgca gctggtcaat taggtaaaat
      421 taaaacagca gttactatgg tagcaattac ttggttgtta ttaggtgatc cattggcaac
      481 attgattggt ttgtcattag gacaaatttt attatacatt ggcgttattt ttactatctt
      541 atctggtatt gaatactttt ataaaggtag agatgttttt aaacaaaaat aaatatttgt
      601 ttatactaga tttcattttc atatggaatc tagttttttt aatcccaatt ttagaaatta
      661 gccacgcaat tgtttataat gatatattgt aaaacaatat ttgttcattt ttttagggaa
      721 aatctgtagt agcatctgat acattgaatc taaaattgat gtgaattttt aaatgaaata
      781 catgaaaaaa tgaattaaac gatacaaggg ggatataaat gtcaattgcc attattgctg
      841 taggctcaga actattgcta ggtcaaatcg ctaataccaa cggacaattt ctatctaaag
      901 tatttaatga aattggacaa aatgtattag aacataaagt tattggagat aataaaaaac
      961 gtttagaatc aagtgtacgt catgcgctag aaaaatatga tactgttatt ttaacaggtg
     1021 gcttaggtcc tacgaaagat gacttaacga agcatacagt ggcccagatt gttggtaaag
     1081 atttagttat tgatgagcct tctttaaaat atattgaaag ctattttgag gaacaaggac
     1141 aagaaatgac acctaataat aaacaacagg ctttagtaat tgaaggttca actgtattaa
     1201 caaatcatca tggcatggct ccaggaatga tggtgaattt tgaaaacaaa caaattattt
     1261 tattaccagg tccaccgaaa gaaatgcaac caatggtgaa aaatgaattg ttgtcacatt
     1321 ttataaacca taatcgaatt atacattctg aactattaag atttgcggga ataggtgaat
     1381 ctaaagtaga aacaatatta atagatctta tcgataaaca gactaatcct acgattgcgc
     1441 ctttggcggg aagtcatgaa gtatatatta gattgactgc aaatgccgac tcaaaagaac
     1501 aagcacaatc attgattcaa cctgttaaac aagaaattct tgatcgtatt ggagaatatt
     1561 attatggttc agatgacaca ttaattgagc aagctgtaat aaagaaaatt catgaacctt
     1621 ttgtaatata tgatggtatt actaatggtg ctttatatca tcgattgaaa gaagtggatt
     1681 taaacgatgt tctaaagggt atgattaatc acaatgaaaa ctttgttgat attaataaac
     1741 ctattgagca gcaattaaaa gatgcagtgc aatttgttaa taaattgttt aatgtgtcat
     1801 cagcaattat tctattagag tatgatggtg tagttcatat aggctatgat aataactttg
     1861 aatttaaaac tgagcaattt aaaatgtcta aatctagaaa tttattaaag aacagaagtc
     1921 aaaattatgc gctcataaga ttattaaatt ggcttagaac aacaaattaa ttgtattatc
     1981 gataaaaata taagcacgtt tgttcgtttt tgcgttttga tttcaagatt ttatacgaac
     2041 aaatattcgc aaaacacttg tattttattt tgaatccttg tatagtattg gtaagataat
     2101 taaaagatag caatttcaat taggaggtct cgctttggat aacgatcgtc aaaaagcttt
     2161 agatacagta attaaaaata tggagaaatc tttcggtaaa ggtgccgtaa tgaagttggg
     2221 tgacaatata ggtcgccgag tttcaactac atcaactggt tcagttacat tagataatgc
     2281 gctaggtgta ggtggctatc ctaaaggacg aattattgaa atttatggtc ctgaaagttc
     2341 tggtaagaca acagtagcgc ttcacgctat tgctgaagta caaagtaatg gcggggtggc
     2401 agcatttatc gatgctgaac atgctttaga tccagaatat gctcaagcat taggcgtaga
     2461 tatcgataat ttatatttat cgcaaccgga tcatggtgaa caaggtcttg aaatcgccga
     2521 agcatttgtt agaagtggtg cagttgatat tgtagttgta gactcagttg ctgctttaac
     2581 acctaaagct gaaattgaag gagaaatggg agacactcac gttggtttac aagctcgttt
     2641 aatgtcacaa gcgttacgta aactttcagg tgctatttct aaatcaaata caactgctat
     2701 tttcatcaac caaattcgtg aaaaagttgg tgttatgttc ggtaatccag agactacacc
     2761 aggtggacgt gcattaaaat tctatagttc agtaagacta gaagtacgtc gtgcagaaca
     2821 gcttaaacaa ggacaagaaa ttgtaggtaa tagaactaaa attaaagtcg ttaaaaataa
     2881 agtggcacca ccatttagag tagctgaagt tgatattatg tatggacaag gtatttctaa
     2941 agagggtgaa cttattgatt taggtgttga aaacgacatc gttgataaat caggagcatg
     3001 gtattcttac aatggcgaac gaatgggtca aggtaaggaa aatgttaaaa tgtacttgaa
     3061 agaaaatcca caaattaaag aagaaattga tcgtaaattg agagaaaaat taggtatatc
     3121 tgatggtgat gttgaagaaa cagaagatgc accaaagtca ttatttgacg aagaatagta
     3181 cacaaattta tatctatagt taaacttagc aaatatcctt ataggattga ttgaaagtga
     3241 tattcatctc ataaagctag aataatatct aactttatgg gatacactac aaatcgagac
     3301 tataaggttt tttattttat ttattattac attatcaata gttttataat cgagcttcaa
     3361 aactttagaa aatagtagaa atagcattca atatagtgca aaagtgcaaa ttgataactt
     3421 gacacttatc tcctataaac cgtacaatta atttgtatga tttatatata atttcataaa
     3481 gtcatattga atttcatata aagagcaaac cctagaaaag gaggtgtttg tgtgaattta
     3541 ttaagcctcc tactcatttt gctggggatc attctaggag ttgttggagg gtatgttgtt
     3601 gcccgaaatt tgttgcttca aaagcaatca caagctagac aaactgccga agatattgta
     3661 aatcaagcac ataaagaagc tgacaatatc aaaaaagaga aattacttga ggcaaaagaa
     3721 gaaaaccaaa tcctaagaga acaaactgaa gcagaactac gagaaagacg tagcgaactt
     3781 caaagacaag aaacccgact tcttcaaaaa gaagaaaact tagagcgcaa atctgatcta
     3841 ttagataaaa aagatgagat tttagagcaa aaagaatcaa aaattgaaga aaaacaacaa
     3901 caagtagatg caaaagagag tagtgttcaa acgttaataa tgaagcatga acaagaatta
     3961 gaacgcatct ccggtctcac tcaagaagaa gctattaatg agcaacttca aagagtagag
     4021 gaagaactgt cacaagatat tgcagtactt gttaaagaaa aagaaaaaga agctaaagaa
     4081 aaagttgata aaacagcaaa agaattatta gctacagcag tacaaagatt agcagcagat
     4141 cacacaagtg aatcaacggt atcagtagtt aacttaccta atgatgagat gaaaggtcga
     4201 atcattggac gagaaggacg aaacatccgc acacttgaaa ctttaactgg cattgattta
     4261 attattgatg acacaccaga agcggttata ttatctggtt ttgatccaat aagaagagaa
     4321 attgctagaa cagcacttgt taacttagta tctgatggac gtattcatcc aggtagaatt
     4381 gaagatatgg tcgaaaaagc tagaaaagaa gtagacgata ttattagaga agcaggtgaa
     4441 caagctacat ttgaagtgaa cgcacataat atgcatcctg acttagtaaa aattgtaggg
     4501 cgtttaaact atcgtacgag ttacggtcaa aatgtactta aacattcaat tgaagttgcg
     4561 catcttgcta gtatgttagc tgctgagcta ggcgaagatg agacattagc gaaacgagct
     4621 ggacttttac atgatgttgg taaagcaatt gatcatgaag tagaaggtag tcatgttgaa
     4681 atcggtgtag aattagcgaa aaaatatggt gaaaatgaaa cagttattaa tgcaatccat
     4741 tctcatcatg gtgatgttga acctacatct attatatcta tccttgttgc tgctgcagat
     4801 gcattgtctg cggctcgtcc aggtgcaaga aaagaaacat tagagaatta tattcgtcga
     4861 ttagaacgtt tagaaacgtt atcagaaagt tatgatggtg tagaaaaagc atttgcgatt
     4921 caggcaggta gagaaatccg agtgattgta tctcctgaag aaattgatga tttaaaatct
     4981 tatcgattgg ctagagatat taaaaatcag attgaagatg aattacaata tcctggtcat
     5041 atcaaggtga cagttgttcg agagactaga gcagtagaat atgcgaaata atttttgtct
     5101 ccctcacaaa ttagtgaggg agctttttta agttgtagtc ttaatctagt tagacagcac
     5161 tttatcggta ataactatat taaacagtag ttatttgaaa gtaagacgga ccttatatta
     5221 aataagaagt tattgctttt aataaaaatg ttttaggctt cgtaattact atatttatat
     5281 tatgtaaacc tataaagatg attgcttttc tatccaataa aaaagaagag aagatgtaac
     5341 acatcttctc ttctgcaata ttaattagga tttatttcta agttgagtta ttttaattgt
     5401 aaatctgttt tctttaattc ttttataact tctgcagtat cataacaatt tgttgcaatt
     5461 gttgaatatc tctctgctaa acgatatgca ttaatgtaaa gctttaaact ttctttagct
     5521 atatcctctg catcttcgaa ttttgatggg ttagacataa ccactaattc tgcaaatttt
     5581 tctggatcaa tattaataga catgtattta tttacaactc ctatttattt tgatgtctta
     5641 atactaacat attgaagttt tcagacaaag taatgtctct ctataattga agaaaaataa
     5701 ttcaaagaat ttaatattta ctttaaatat tagaacgtat gaattaaact agtatttatg
     5761 agaggatgaa caaaacatga gaataatgtt tataggggat atcgtaggta aaattggacg
     5821 agacgcaatt gaaacgtaca tacctcaact gaagcaaaag tataaaccaa cagttacaat
     5881 tgtaaatgct gaaaatgcag cacatggtaa aggtttgact gaaaaaatat ataaacaatt
     5941 actaagaaat ggtgtagatt tcatgactat gggtaatcac acatatggtc aacgtgaaat
     6001 ttatgatttt atagatgaag caaaacgact agtaagacca gcgaattttc cggatgaagc
     6061 gccgggaatt ggtatgagat ttatacaaat taatgatatt aaacttgcag ttattaatct
     6121 gcaaggaaga gcgtttatgc cagatattga tgatcctttt aaaaaggcag atcaattagt
     6181 caaggaagca caagaacaaa ctccgtttat atttgttgat tttcatgcag aaacaacttc
     6241 tgaaaagtat gcaatgggat ggcatttaga tggtagagct agcgctgttg ttggaacgca
     6301 tacacacatt caaacagcag atgaacgtat tttaccaaag gggacagggt atataacgga
     6361 tgttggtatg acaggttttt atgatggcat tttaggaata aataaaacag aggtaattga
     6421 gcgttttatc actagtttgc cacaaagaca tgttgttcca aatgaaggta gaagtgtatt
     6481 atctggtgtt gttattgatt tagacaaaga aggtaaaaca aagcacatcg aacgtatatt
     6541 gataaatgat gaccatccat tttcaacatt ttaaaattac gtaagtaaac attcgaattg
     6601 gaccctatcg tccattagta tgaatttaat atagtaccac tgtttacata gtaaatcggt
     6661 ggttcttttt gttatcattt aatatgaaat atatccatag gaggcatata actatgaaac
     6721 cacaattatc gtggaaagtt ggcggtcaac aaggcgaagg tattgaatca actggggaaa
     6781 tcttcgctac ggctatgaat agaaaaggat attatttata tggatataga catttttcaa
     6841 gtcgtatcaa aggtggacat acgaataata aaattagagt ttctacgacg cctgttcatg
     6901 caattagtga tgatttagat attttgattg catttgacca agaaacaatt gatgttaacc
     6961 atcatgaaat gagagaagac agtattattt tagctgatgc caaggctaaa cctgtgaaac
     7021 cagaaggatg tcatgcacag cttattgaat taccttttac agcaaccgct aaagaattag
     7081 gtacagcatt aatgaaaaac atggttgcaa taggtgctac tagcgcattg atgaatttga
     7141 atacaaatac atttgaagaa cttattacta atatgttttc taaaaaaggt gacaaggtag
     7201 ttgaagtcaa tatccaagca ttaaacgaag gttatcaatt aatgcaatct cgcttacctg
     7261 aaatctacgg ggactttgaa ttagagtcaa cagatgcact accacatcta tatatgattg
     7321 gtaacgatgc cattggatta ggtgcaattg ctgcaggttc acaatttatg gcggcatatc
     7381 ctattacacc tgcgtctgaa gttatggaat atatgattgc caatatatct aaagtaaacg
     7441 gagcggttat tcaaacagaa gatgaaattg ctgctgtaac tatggctatt ggtgcaaatt
     7501 atggtggagt cagagcgttt acggctagtg ctggtccagg tttatcttta atgatggaag
     7561 caattggatt atctggtatg actgaaacgc cattagtcat tattaatacc caacgaggtg
     7621 gaccttctac tggattacct acgaaacaag aacagtcaga tttaatgcaa atgatttatg
     7681 gtacacatgg tgatattcca aaaattgttg tagcaccaac agatgcagaa gatgcatttt
     7741 atttaactat ggaagcattt aatttagcag aacaatatca atgccctgtt atagttctaa
     7801 gtgatttgca attatcttta ggtaaacaaa ctgttgaaaa attagattat aatcgtattg
     7861 aaattaaacg tggtgaaatc attcaatctg atattgaacg tgaagaagat gataaaggtt
     7921 atttcaagcg ttatgcgtta acatccgatg gtgtttctcc tagacctatc cccggtgtta
     7981 aaggaggtat tcatcatata actggtgtgg aacacaatga agaaggtaaa cctagtgaat
     8041 ctgcgtcaaa tagacaacaa caaatggaaa aacgaatgcg taaaattgag cagttactaa
     8101 ttgaatcgcc agtagaagct aacttacaac atgaggatgc agatattctt tatatcggtt
     8161 ttatttctac aaaaggtgca attcaagaag gtagtaaccg tttgaatcaa caaggcataa
     8221 aagttaacac tatacaaatt agacaattgc atccattccc aacaagcgtt attcaagatg
     8281 cagttaataa agcgaagaaa gtcgttgtag tggagcacaa ttatcaagga caattggcta
     8341 gtattataaa aatgaatgtc aatattcatg ataagattga aaattataca aagtatgatg
     8401 ggacaccttt cctaccacat gaaatcgaag aaaaaggcaa aataattgct actgaaataa
     8461 aggagatggt atagatggcg acatttaaag attttagaaa taatgttaag cctaactggt
     8521 gccccggatg tggcgatttc tcagtacaag ctgcaattca aaaagcagcc gcaaatatag
     8581 ggttagaacc tgaagaagta gctatcatca ccggtatagg atgttctggc cgtctttcag
     8641 gatatattaa ttcttatggc gttcattcta ttcacggacg tgcattacct ttagctcaag
     8701 gtgtaaaaat ggcgaataaa gatttaactg ttattgcatc gggaggagat ggtgatggtt
     8761 atgctatagg tatggggcat acaatccatg ctttaagaag aaatatgaac atgacgtata
     8821 tagtcatgga taatcaaatt tatggtttga caaagggaca aacatcgccg tcatcagcag
     8881 taggatttgt tactaaaaca acgccaaaag gtaatataga aaaaaatgtt gcgcctttag
     8941 aattagcatt atcatctggt gccacatttg tagcccaagg tttttcaagc gatattaaag
     9001 gattaacaaa actaattgaa gatgcaatta atcatgatgg attttcattc gttaatgtct
     9061 tttcaccatg tgtgacttat aataaaatta acacatacga ttggtttaaa gaacatttaa
     9121 caagtgttga tgacattgaa aattatgatt ctacagataa acaattagcg actaaaactg
     9181 ttattgaaca tgaatcttta gtaactggta ttgtttatca agataaagaa acaccatcat
     9241 atgaatctca aattaaagag ttagatgata caccacttgc taaaagagat atcaaaatta
     9301 ctgaagacac cttcaatgca ttaactgaac aatttattta ataattaaaa ctgagtcgta
     9361 taaatgtatt tataacagat ccatttatgc tactcagttt tttactatta caaaaaataa
     9421 aggagttttt aaaaatgaaa gacacattaa tgagtataca aataattcct aaaacaccaa
     9481 acaatgacaa tgttatacct tacgtagacg aggcgattaa aataattgac gaatctggtt
     9541 tgcattttag agtaggtccg ttagaaacga cagtacaagg aaatatgaat gaatgtttaa
     9601 ttttaataca atcattaaat gaacgaatgg tggaacttga atgtccaagt attattagcc
     9661 aagttaagtt ttatcatgtg ccagatggca tcactattga aactttaact gaaaaatatg
     9721 atgaataaca ttaaaagtga agtaaactgg atttgaattg gcttgttaga gatgacgtat
     9781 aactttaact gtttttgcac tttatagtta aatttaatat aattattaaa tgatacgggc
     9841 aaatagaaag gattttgtaa agtgaacgaa gaacaaagaa aagcaagttc tgtagatgtt
     9901 ttagctgaga gagataagaa agcagaaaaa gattatagta aatattttga acatgtttat
     9961 cagccgccta atttaaaaga agcgaaaaaa agaggtaaac aagaagttcg ttataataga
    10021 gatttccaaa ttgatgaaaa atatcgcggt atggggaacg agcgtacatt tttaattaaa
    10081 acatatggat gtcaaatgaa tgcacatgac actgaggtca ttgctggtat acttgaagca
    10141 ttaggctatc aagcaacgac tgatattaac actgcagatg ttattttaat taatacatgt
    10201 gcgattagag aaaatgccga gaacaaagtg tttagtgaaa taggtaattt gaagcatttg
    10261 aaaaaagaac gacctgatat tttaatcggt gtttgtggtt gtatgtcaca agaagagtca
    10321 gtagtgaata aaattttaaa atcgtatcaa aatgtagata tgatatttgg tacacataat
    10381 attcatcatt taccagaaat tttagaagaa gcatacttat ctaaagcaat ggttgttgaa
    10441 gtatggtcta aagaaggaga cgttattgaa aatcttccaa aagtccgtga aggcaacatt
    10501 aaagcatggg tcaatattat gtatggttgt gataagtttt gtacatattg tattgttcca
    10561 tttacaagag gtaaagaacg aagccgtaga cctgaagaca ttatagatga agtacgtgaa
    10621 cttgctcgtg aaggttacaa agaaataacg cttttaggtc aaaatgtaaa ttcttatggt
    10681 aaagatttac aggatataga atatgactta ggagatcttt tacaagcaat ttctaaaata
    10741 gcgattccaa gagttcgttt cacaacaagt catccttggg actttacaga tcacatgatt
    10801 gatgttattt cagagggtgg taatatcgtt ccgcatatcc acttgccagt tcaatctgga
    10861 aataatgcag tattaaaaat aatgggtaga aaatatacac gagaaagtta tttggattta
    10921 gtaaaacgaa tcaaagatag aattcctaat gtagcattaa ctacagatat tattgtaggg
    10981 tatccaaatg aatcagagga acaatttgaa gaaactttaa ctctgtatga tgaagttggt
    11041 tttgaacatg catatacgta cttgtattca caacgtgatg gtacgcctgc tgctaaaatg
    11101 aaagataatg tacctttaaa tgtcaaaaag gaacgattgc aacgtttgaa taaaaaagtt
    11161 ggtcattatt cacaaatagc tatgagtaag tacgaaggac aaactgtaac agtactttgt
    11221 gaaggaagta gtaaaaaaga tgatcaggtt cttgctggct acactgataa aaataagcta
    11281 gttaatttca aagcgcctaa agaaatgatt ggtaaactag tggaagtacg aatagatgaa
    11341 gctaaacagt attcattaaa tggcagtttt ataaaggaag tagagccgga aatggtgatt
    11401 caataaatgt ataataaaga tgacgtgttg aaccaagcgg ataatattgc aaataaaatt
    11461 aaaaatttgg atactatcaa aacatatcaa caaattgaag cacagattca tcagaaccaa
    11521 acgataaaga ctaaaatgga tatgttaaaa aagcatcaaa aacaagcagt aaactttcaa
    11581 aattacggga aacaaaatgc gctagaacag tcggaacata ccattcagag tatagaagca
    11641 gaaataaata cattgcccat agttgaacag tttcaaactt cacaatatga agcgaatcaa
    11701 ttattgaaaa tgtttgtatc aacaatggaa acacgtttaa atgaccataa taaagccaag
    11761 catagtgatt aattacaaac aaaaaggaga acaatcatat gaaatcaaga aaactggcta
    11821 taactgcact tttaattgca ataaatgttg tattaagcag tattatcatc attcctctag
    11881 gaccagttaa ggcagcacca gtacagcatt ttgtaaatgt attaagtgcg gtcatagtag
    11941 gtccttggta tggattagct caagcgctta tatcatcaat tttaagagtt ctttttggta
    12001 ctggtacagc ttttgcattt ccgggtagta tgattggagt tttattggct agtatgtttt
    12061 acatatatcg taaacatata tttatggccg cggtcggtga agtacttgga actggtgtca
    12121 tcggaagttt aatttgtata ccattagcat atttccttgg gcttcaagac ttcttcatta
    12181 aaccgttaat gattacgttc atagtctcaa gtgctatcgg atctattata agttatttct
    12241 tattaattac tctaaaaaaa cgtggtattc ttcaaaggtt tataaaataa ttttatgata
    12301 tatgatttca aacagagtca ttgttcattt taatattgaa cgcccttact taattaaatg
    12361 tcttgaatgt tagaatttta atttaattct aaacatgcta ttgacacatt ttattaggtt
    12421 tgaggcgttt tcttatacat ttaagatgct tcgtgattat atggtatgaa taggtttgta
    12481 aaagtaagta tgttaaaatg tataagttaa ataattaata attaaaaagc ttacaaacga
    12541 atatattgta aagacaaatt ttaaaaagag acaagggttg tgaagaaata atatgtctaa
    12601 tgttacacca atgatgcagc aatatttaaa aataaaatca gaataccaag attgcttatt
    12661 attttttaga ctaggtgatt tctatgaaat gttttatgaa gatgccaagg aggcatcacg
    12721 tgtacttgaa attactttaa ctaaaagaga tgctaaaaaa gaaaatccaa ttccgatgtg
    12781 tggtgttccg tatcattctg cagatagtta tatagataca cttgttaata atggatataa
    12841 agtagctatt tgtgaacaga tggaagatcc gaaacaaacg aaaggtatgg ttagacgtga
    12901 ggtagtaaga attgtgactc caggaactgt gatggagcaa ggtggtgtag atgataaaca
    12961 aaataactat attttaagtt ttgttatgaa tcaacctgaa attgcgctta gttactgtga
    13021 tgtttctact ggcgaattaa aggttacaca ttttaatgat gaagcgactt tattaaatga
    13081 aattacgacg ataaacccta acgaagttgt tatcaatgac aatatttccg ataatttaaa
    13141 aagacaaatt aatatggtga cagaaacaat aacagtcagg gaaacgttat catcagaaat
    13201 ctatagtgtg aatcaaactg aacataaatt aatgtatcaa gcgacacaat tattgctaga
    13261 ttatattcat catacacaaa aacgtgattt atcgcatatc gaggatgttg ttcaatatgc
    13321 agctatagat tatatgaaaa tggattttta tgctaagaga aaccttgagt taacggaaag
    13381 cattcgatta aaatcaaaaa aaggaacgct actttggcta atggacgaaa cgaaaacacc
    13441 aatgggagca cgccgcttaa aacaatggat agatagacca ctaataagta aagaacaaat
    13501 tgaagcacga ttagatatcg ttgatgaatt tagtgctcat ttcatagaaa gagacacctt
    13561 aagaacatat cttaatcaag tgtatgatat tgaacgtctt gttgggcgtg ttagttacgg
    13621 aaatgttaat gcgagagatt taattcaact taaacattcc atttctgaaa taccgaatat
    13681 taaagcatta ctaaattcta tgaatcagaa tactcttgta caagttaatc aactagaacc
    13741 ccttgatgat ttacttgata tattagaaca gagtttagta gaagaaccac caatttcagt
    13801 taaagatggc ggactattca aagttggttt taatacgcaa ttagatgaat atcttgaagc
    13861 ttcaaaaaac ggaaaaacat ggttagcaga attacaagcc aaagaaagac aacgtacagg
    13921 aataaaatca ttgaaaataa gctttaataa agtgtttggt tattttatag aaataacacg
    13981 tgccaacttg caaaattttg aaccaagtga atttggttat atgaggaagc aaacgttatc
    14041 gaatgctgaa cgttttataa ctgatgaact taaagaaaaa gaagatatca ttttaggtgc
    14101 ggaagacaaa gccatcgaat tagaatatca attatttgtt cagctacgtg aagaagttaa
    14161 aaaatatact gaacgtttac aacaacaagc taaaattatt tcagagctag attgtttaca
    14221 gagctttgca gaaattgctc aaaaatataa ttacactagg ccttcattta gtgaaaataa
    14281 aacattagaa ttagtggaat ctaggcaccc agtagtggaa agagtaatgg attataatga
    14341 ctatgtgcct aataattgtc gattagataa tgaaacattt atatatttaa ttacaggtcc
    14401 gaatatgtct ggtaaatcga catatatgag acaagttgcc ataattagta taatggccca
    14461 aatgggagct tatgtccctt gtaaagaggc agtgttacct atatttgatc aaatattcac
    14521 tagaataggt gcggcagatg atttggtttc aggtaagagt acgtttatgg tagaaatgct
    14581 agaagcacaa aaggcattaa cttatgcaac agaggatagt ttgattattt tcgatgaaat
    14641 tggacgtggt acttcaacgt atgacggttt agctttagcg caggcaatga tagagtatgt
    14701 agctgaaaca tcacatgcta aaacgttatt ttcaacacat tatcatgaat tgacaacatt
    14761 agatcaagca ttaccaagtc taaaaaatgt tcacgtcgct gctaatgaat ataaaggtga
    14821 acttatattc ttgcataaag tcaaagatgg tgcagttgac gatagttatg gtattcaagt
    14881 tgcgaaatta gctgatttac ctgaaaaagt tattagcaga gcacaagtga ttctaagcga
    14941 gtttgaagcg tctgctggta aaaaatcatc gatatcaaat ttaaaaatgg tcgaaaatga
    15001 acctgaaatt aatcaagaaa atttaaactt aagtgttgaa gaaacaactg atactttatc
    15061 tcaaaaagac tttgaacaag catcatttga tttgtttgaa aatgatcaag aaagcgagat
    15121 tgaactacaa attaaaaatt tgaatttatc taatatgaca ccaattgagg cattggtgaa
    15181 gttaagtgaa ttacaaaatc aattaaaata gaggtgttgc aaaatgggga aaattaaaga
    15241 actccaaacc tcattagcaa ataaaatcgc agcaggtgaa gtagttgaaa gaccgagttc
    15301 tgttgtgaaa gaactgttgg aaaatgctat agatgcaggc gctacagaaa taagcattga
    15361 agtagaggaa tctggcgtcc aatctattcg cgtagtcgat aatggaagcg gaattgaagc
    15421 ggaagactta ggattagtat ttcatagaca tgcgactagt aaattagatc aagatgaaga
    15481 tttatttcat attaggacat taggattccg tggtgaagca ctagccagta tttcatcagt
    15541 tgctaaagta acattgaaga cttgcacgga taatgctaat ggaaatgaaa tatatgtaga
    15601 aaatggtgaa atattaaatc ataagcctgc aaaagcgaaa aaaggaacag atatacttgt
    15661 agaatcatta ttttataata caccagcacg tttaaaatat attaaaagtt tatacactga
    15721 actaggtaaa ataacagata ttgtcaacag aatggcaatg agccatccgg acattcgaat
    15781 agcactcatt tcagatggca aaacaatgtt aagtacaaat ggttcaggac gaactaatga
    15841 agtgatggca gagatttatg ggatgaaagt tgcacgagat ttagtacata tatctggaga
    15901 tacaagtgat tatcacattg aaggttttgt tgcaaagcct gaacattcta gaagtaataa
    15961 gcactatatt tctattttta ttaatggacg atacattaaa aactttatgc taaataaagc
    16021 gattttagaa ggctatcata cactcttaac aataggtagg ttcccgattt gttatattaa
    16081 tattgaaatg gatccaatct tagtagacgt aaatgttcat ccaacaaaac tagaagtgcg
    16141 tttatcaaaa gaagagcaac tataccaatt gattgtgagc aaaatacaag aagcatttaa
    16201 agaccgtata ttaattccta aaaataactt ggattatgtg ccaaaaaaaa ataaagtatt
    16261 acattcattc gaacaacaaa aaatcgaatt tgaacaaaga caaaacacag agaataacca
    16321 agagaagacg ttttcatctg aagaaagtaa cagtaagcca tttatggaag aaaatcaaaa
    16381 cgatgagata gttataaaag aagattcata taatccattc gtaacgaaaa catctgaaag
    16441 tttaatagct gatgatgaat cttctggtta taataataca cgtgaaaaag atgaagacta
    16501 cttcaaaaaa caacaagaaa ttttacaaga aatggatcaa acatttgatt caaatgatgg
    16561 tacaactgtg caaaattatg agaataaagc gtctgatgat tattatgatg taaacgatat
    16621 taaaggaaca aaaagtaaag accctaaacg aagaattcca tatatggaaa ttgttggaca
    16681 agtacatgga acgtatatta ttgctcaaaa tgaatttggc atgtacatga ttgaccagca
    16741 tgcagctcaa gaaagaataa aatatgaata ttttcgagat aaaataggtg aggttaccaa
    16801 tgaagtacaa gatttattaa tcccgttaac atttcatttt tcaaaagatg aacaattagt
    16861 cattgatcaa tataaaaatg agcttcaaca agtaggtatc atgttagaac attttggtgg
    16921 tcatgattat attgtaagta gctatccagt ttggttccct aaagatgaag tagaagaaat
    16981 tattaaagat atgattgagc taattttgga agagaaaaaa gtagatatca aaaaattacg
    17041 tgaagatgta gcaatcatga tgtcatgtaa aaaatccatt aaagcgaatc attatttaca
    17101 aaaacatgaa atgtctgatt taattgatca attaagagaa gcggaagatc catttacatg
    17161 tccacatggt cgtccaataa tcattaattt ttcaaaatac gaattagaaa aattatttaa
    17221 gcgtgtgatg tagagaggat gaatcaagtg aataacaaca tattgcctgc tataagaaac
    17281 attaaagatt tagagaaact gattaaaaca gactataaaa tgtgtgtgct tctagatatg
    17341 catataggac atataaaaag tattatggaa ttgctgaagc aaaatcatat agagtgtttt
    17401 attcatatag atttgataaa aggtttaagc cacgatgaat ttgcaagtga atttattatt
    17461 cagcaataca agccaaaagg tatcgtatcg actaaatcta aagtaa
//
LOCUS       DANJFZ010000051        17351 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.051, whole genome shotgun sequence.
ACCESSION   DANJFZ010000051 DANJFZ010000000
VERSION     DANJFZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.051"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            187..828
                     /locus_tag="PWN45_001497"
     CDS             187..828
                     /locus_tag="PWN45_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0623266.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            920..1375
                     /locus_tag="PWN45_001498"
     CDS             920..1375
                     /locus_tag="PWN45_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDL0623267.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            1433..1783
                     /locus_tag="PWN45_001499"
     CDS             1433..1783
                     /locus_tag="PWN45_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623268.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            1825..1983
                     /locus_tag="PWN45_001500"
     CDS             1825..1983
                     /locus_tag="PWN45_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623269.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            1997..8197
                     /locus_tag="PWN45_001501"
     CDS             1997..8197
                     /locus_tag="PWN45_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0623270.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            8197..9021
                     /locus_tag="PWN45_001502"
     CDS             8197..9021
                     /locus_tag="PWN45_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDL0623271.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            9030..10613
                     /locus_tag="PWN45_001503"
     CDS             9030..10613
                     /locus_tag="PWN45_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0623272.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            10613..10903
                     /locus_tag="PWN45_001504"
     CDS             10613..10903
                     /locus_tag="PWN45_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623273.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            10919..12829
                     /locus_tag="PWN45_001505"
     CDS             10919..12829
                     /locus_tag="PWN45_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623274.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            12829..14295
                     /locus_tag="PWN45_001506"
     CDS             12829..14295
                     /locus_tag="PWN45_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL0623275.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            14295..14684
                     /locus_tag="PWN45_001507"
     CDS             14295..14684
                     /locus_tag="PWN45_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDL0623276.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            14677..14841
                     /locus_tag="PWN45_001508"
     CDS             14677..14841
                     /locus_tag="PWN45_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0623277.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            14887..15186
                     /locus_tag="PWN45_001509"
     CDS             14887..15186
                     /locus_tag="PWN45_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDL0623278.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            15322..15624
                     /locus_tag="PWN45_001510"
     CDS             15322..15624
                     /locus_tag="PWN45_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0623279.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            15635..17089
                     /locus_tag="PWN45_001511"
     CDS             15635..17089
                     /locus_tag="PWN45_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0623280.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT         6568 a         2362 c         3588 g         4833 t
ORIGIN      
        1 aacgaataag atacctgtta tatcaacaaa atttaattca agcatctagt cagttagatg
       61 cttattttga agaaactaaa cgttatgtga tgtcgagacg atatcaaggc atacccaaaa
      121 atatatatta taaaaatcag cgcatcgaat aggtgtgctt tttaattttt aaggaggaaa
      181 taagcaatgg cagaaggaca aggttcttat aaagtaggtt ttaaaagatt atacgttgga
      241 gtttttaacc cagaagcaac aaaagtagtt aaacgcatga catgggaaga tgaaaaaggt
      301 ggtacagttg acctaaatat cacaggttta gcaccagatt tagtagatat gtttgcatct
      361 aacaaacgtg tatggatgaa aaaacaaggt actaatgaag ttaagtctga catgagtatt
      421 ttcaatattc caagtgatga tttaaacaca gttattggac gtactaaaga taaaaatggt
      481 acatcttggg taggagagaa tacaagagca ccgtatgtaa cagtaattgg cgaatcggaa
      541 gatggtttaa caggtcagcc ggtatatgta gccttactta aaggtacttt tagtttagat
      601 tcaattgaat ttaaaacacg aggtgaaaaa gcagaagccc cagaacctac aaaattaaca
      661 ggtgactgga tgaatagaaa agttgatgtt gatggaacgt cacaaggtat tgtatacggt
      721 tatcatgaag gtaaagaagg agaagcagaa ttcttcaaaa aagtattcgt tggatacacg
      781 gacagtgaag atcattcaga ggattctgca ggttcgttac ccagctaatc cccaaaatgt
      841 tgaagtagca gttaattcaa aatctgcaac agtttcagca gaataggggc tttcaaaata
      901 aatcaaagga gaataattta tgactaaaac tttaaaggtt tataaaggag acgacgtcgt
      961 agcttctgaa caaggtgaag gcaaagtgtc agtaacttta tctaatttag aagcggatac
     1021 aacttatcca aaaggtactt accaagtggc atgggaagaa aatggtaaag aatctagtaa
     1081 agttgatgta cctcaattca aaaccaatcc aattctagtc tcaggcgtat catttacacc
     1141 agaaactaaa tcaattatgg taaataccga tgacaatgtt gagccaaaca ttgcaccaag
     1201 cacagcaacg aataaaatat tgaaatatac aagtgaacat ccagaatttg ttactgtaga
     1261 tgaaaataca ggagcaattc acggtgtagc tgaaggtact tcagtaatca ctgctatgtc
     1321 tactgatgga agcgataagt caggacaaat ttcagtgaca gtaacaaacg gatagggatt
     1381 taaggcgcag tatatctgcg tcttttttat ttgaataaaa ggagctaata caatgattaa
     1441 atttgaaatt aaagatcgta aaacaggaaa aacagagagc tatacaaaag aagatgtaac
     1501 aatgggcgaa gcagaaaaat gctatgagta tttagaatta gtaaatcaag agaataaaaa
     1561 agaagcacct aacgcaacaa aaatgagaca aaaagagcga cagttattag tagatttatt
     1621 taaagatgaa ggattgactg aagaagatgt tctgaacaag atgagtacta aaacttatac
     1681 aaaagcctta caagatatat ttcgagaaat caatggtgaa gatgaagaag attcagaaac
     1741 tgaaccagaa gagatgggaa agacagaaga acaatctcaa taaaagacat tttatcgaac
     1801 attaagaaaa tacaacgttt ctgtatggag cagtatgggt ggacattaac tgaagtcaga
     1861 aaacagccgt atgtaaaact tttagaaata cttaatgaag agaataaaga agagactgaa
     1921 gaaaaacaaa gtgaacaaaa agtcattaca ggtacggatt taagaaaact ttttggaagc
     1981 tagaaaggag gttaatatga atgaaaaagt agaaggcatg accttggagc tgaaattaga
     2041 ccatttaggt gtccaagaat gcatgaaagg tttaaagcga caattaggtg ttgttaatag
     2101 tgaaatgaaa gctaatctgt cagcatttga taagtctgaa aaatcaatgg aaaaatatca
     2161 ggcgagaatt aaggggttaa atgataggct taaagttcaa aaaaagatgt attctcaagt
     2221 agaagatgag cttaaacaag ttaacgctaa ttaccaaaaa gctaaatcca gtgtaaaaga
     2281 tgttgagaaa gcatatttaa agttagtaga agccaataaa aaagaaaaat tagctcttga
     2341 taaatctaaa gaagccttaa aatcatcgaa tacagaactt aaaaaagctg aaaatcaata
     2401 taaacgtaca aatcaacgta aacaagatgc gtatcaaaaa cttaaacagt tgagagatgc
     2461 agaacaaaag cttaagaata gtaaccaagc tactactgca caactaaaaa gagcaagtga
     2521 cgcagtacag aagcagtccg ctaagcataa agcacttgtt gaacaatata aacaagaagg
     2581 caatcaagtt caaaaactaa aagtgcaaaa tgacaatctt tcaaaatcaa atgataaaat
     2641 tgaaagttct tacgctaaaa ctaatactaa attaaagcaa acagaaaaag aatttaatga
     2701 tttaaacaat actattaaga atcatagcgc taatgtcgca aaagctgaaa cagctgttaa
     2761 taaagaaaaa gctgctttaa ataatttgga gcgttcaata gataaagctt catccgaaat
     2821 gaagactttt aacaaagaac aaatgatagc tcaaagtcat ttcggtaaac ttgcaagtca
     2881 agcggatgtc atgtcaaaga aatttagttc tattggagac aaaatgactt ccctgggacg
     2941 tacaatgacg atgggcgtat ctacaccaat tactttaggg ttaggtgcag cattaaaaac
     3001 aagtgcagac tttgaaggcc aaatgtctcg agttggagcg attgcgcaag caagcagtaa
     3061 agacttgaaa agcatgtcta atcaagcagt tgacttagga gctaaaacca gtaaaagtgc
     3121 taacgaagtt gctaaaggta tggaagaatt ggcagcttta ggctttaatg ccaaacaaac
     3181 aatggaggct atgccaggtg ttatcagtgc agcagaagca agtggtgcag aaatggctac
     3241 aactgcaact gtaatggctt cagcgattaa ctctttcggt ttaaaagcat ctgatgcaaa
     3301 tcatgttgct gatttacttg cgagatcagc aaatgatagt gctgcagata ttcagtacat
     3361 gggagatgca ttgaagtatg ctggtactcc tgcaaaagca ttaggagttt caatagagga
     3421 cacttccgca gcaattgaag ttttatctaa ctcaggttta gagggttctc aagcaggtac
     3481 tgccctaaga gcttcattta tcaggctagc taatccaagt aaaaatacag ctaaggaaat
     3541 gaaaaaatta ggtattcatt tgtctgatgc taaaggtcaa tttgttggca tgggtgaatt
     3601 gattagacag ttccaagata atatgaaagg catgacgaga gaacaaaaac tagctacagt
     3661 ggctacaata gttggtactg aagcagcaag tggattttta gccttgattg aagcgggacc
     3721 agataaaatt aatagctata gtaaatcctt aaagaattcc aatggcgaaa gtaaaaaagc
     3781 agcagatttg atgaaagata atctcaaagg cgctctggaa caattaggtg gcgcttttga
     3841 atcattagca atcgaagtcg gtaaagattt aacgcctatg attagagcag gagcggaagg
     3901 tttaacaaaa ttagttgatg gatttacaca tctccctggt tgggttagaa aagcttcagt
     3961 aggattagca ctttttggtg cagcaattgg acctgcagtt cttgctggag ggttattaat
     4021 acgtacagtt ggaagtgctg ctaaaggata tgcgtcatta aatagacgta ttgctgaaaa
     4081 tacaatcctt tcaaatacta attcaaaagc aatgaaatct ttaggtcttc aaacattatt
     4141 tcttggttct acaacaggaa aaacgtcaaa aggctttaaa gggttagccg gagctatgat
     4201 gtttaattta aaacctataa atgttttgaa aaattctgca aagctagcaa ttttaccgtt
     4261 caaacttttg aaaaacggtt taggattagc tgcaaaatct ttatttgcag taagtggagg
     4321 cgcaagattt gcgggtgtag ccttaaggtt tttaacagga cctataggtg ctacaataac
     4381 tgctattaca attgcgtata aagtttttaa aaccgcatat gatcgtgtgg aatggttcag
     4441 aaacggtatt aacggtttag gagaaactat aaagtttttt ggtggtaaaa ttattggcgg
     4501 cgctgttaga aagctaggag agtttaaaaa ctatcttgga agtatcggca aaagcttcaa
     4561 agaaaagttt tcaaaagata tgaaagatgg ttataaatca ttaagcgacg atgaccttct
     4621 caaagtagga gtcaacaagt ttaaaggatt tatgcaaacc atgggcacag cttctaaaaa
     4681 agcgtctgat actgtaaaag tgttagggaa aggtgtttca aaagaaacag aaaaagcttt
     4741 agaaaaatat gtgcattatt ctgaagaaaa tagcagaatc atggaaaaag tacgtttaaa
     4801 ctcgggtcag atatcagaag acaaagcaaa aaaacttttg aaaattgaaa cggatttatc
     4861 taataacctt atagctgaaa tagaaaaaag aaataaaaag gaactcgaaa aaactcaaga
     4921 acttattgat aagtatagtg cattcgatga acaagaaaag caaaacattt taactcgaac
     4981 taaagaaaaa aatgacttgc gaattaaaaa agagcaagaa ctcaatcaga aaatcaaaga
     5041 attgaaagaa aaagctttga gtgatggtca gatttcagaa aatgaaagaa aagaaattga
     5101 aaagcttgaa aatcaaagac gtgatatcac tgttaaagaa ttgagtaaga ctgaaaaaga
     5161 gcaagagcgt attttagtaa gaatgcaaag aaacagaaat gcttattcaa tagacgaagc
     5221 gagcaaagca attaaagaag cagaaaaagc aagaaaagca agaaaaaaag aagtggacaa
     5281 gcaatatgaa gatgatgtca ttgctataaa aaataacgtc aacctttcta agtctgaaaa
     5341 agataaattg ttagctattg ctgatcaaag acataaagat gaagtaagaa aagcaaaatc
     5401 taaaaaagat gctgtagtag atgttgttaa aaagcaaaat aaagatattg ataaagaaat
     5461 ggatttatcc agtggacgtg tatataaaaa tactgaaaag tggtggaatg gccttaaaag
     5521 ttggtggtct aactttagag aagaccaaaa gaaaaaaagc gataaatacg ctaaagaaca
     5581 agaagaaaca gctcgtagaa acagagaaaa tataaagaaa tggtttggaa atgcttggga
     5641 cggcgtaaaa agtaaaactg gcgaagcctt tagtaaaatg ggcagaaatg ctaatcattt
     5701 tggcggcgaa atgaaaaaaa tgtggagcgg aatcaaaggg attccaagca aattaagttc
     5761 aggttggagc tcagccaaaa gttctgtagg ataccacact aaggctatag ctaatagtac
     5821 tggtaaatgg tttggaaaag cttggcaatc tgttaaatcg acaacaggaa gtatttacaa
     5881 tcaaactaag caaaagtatt cagatgcttc agataaagct tgggcgcatt caaaatctat
     5941 ttggagaggc acatcaaaat ggtttagcaa tgcatataaa agtgcaaagg gctggctaac
     6001 ggatatggct aataaatctc gcgcgaaatg ggataatatt tctagtacag cttggtcgaa
     6061 tgcaaaatcc gtttggaaag gaacatcgaa atggtttagt aactcataca aatctttaaa
     6121 agattggact ggggatatgt attcaagagc ccacgatcgt tttgatgcaa tttcaagttc
     6181 ggcatggtct aacgctaaat cagtatttaa tggttttaga aaatggctat ccaaaacata
     6241 tgattggatt agagatattg gtaaagacat gggaagagct gcggctgatt taggtaaaaa
     6301 tgttgctaat aaagctattg gcggtttgaa tagcatgatt ggcggtatta ataaaatatc
     6361 taaagccatt actgataaaa atctcatcaa gccaatacct acattgtcta ctggtacttt
     6421 agcaggaaag ggtgtagcta ccgataattc gggagcatta acgcaaccga catttgctgt
     6481 attaaatgat agaggttctg gaaacgcccc aggtggtgga gttcaagaag taattcacag
     6541 ggctgacgga acattccatg caccccaagg acgagatgtg gttgttccac taggagttgg
     6601 ggatagcgta ataaatgcta atgacactct gaagttacag cgtatgggtg ttttaccaaa
     6661 gtttcatgga ggtacgaaaa agaaagattg gctagaccaa cttaaaggta atataggtaa
     6721 aaaagcagga gaatttggag ctacagctaa aaacacagcg cataatatca aaaaaggtgc
     6781 agaagaaatg gttgaagcag caggcgataa aatcaaagat ggtgcatctt ggttaggcga
     6841 taaaatcggc gatgtgtggg attacgtaca acatccaggg aaactagtaa ataaagtaat
     6901 gtcaggttta aatattaatt ttggaggcgg agctaacgct acagtaaaaa tagctaaagg
     6961 cgcatactca ttgctcaaaa agaaattagt agacaaagta aaatcgtggt ttgaagattt
     7021 cggtggtgga ggcgatggaa gctatctatt tgaatatcca atctggcaaa gatttggacg
     7081 ctacacaggt ggacttaact ttaatggcgg tcgtcactat ggtatagact ttggtatgcc
     7141 ttctggaaca aacgtttatg ccgttaaagg tggtatagca gataaggtat ggactgatta
     7201 cggtggcggt aattctatac aaattaagac tggtgctaat gaatggaact ggtatatgca
     7261 tttatctaag caattagcaa gacaaggcca acgtattaaa gctggtcaac tgatagggaa
     7321 atcaggtgct acaggtaatt tcgttagagg agcacactta catttccaat tgatgcaagg
     7381 gtcacatcca gggaatgata cagctaaaga tccagaaaaa tggttgaagt cacttaaagg
     7441 tagtggcgtt cgaagtggtt caggtgttaa taaggctgca tctgcttggg caggcgatat
     7501 acgtcgtgca gcaaaacgaa tgggtgttaa tgttacttcg ggtgatgtag gaaatattat
     7561 tagcttgatt caacacgaat caggaggaaa tgcaggtata actcaatcta gtgcgcttag
     7621 agacatcaac gttttacagg gcaatccagc aaaaggattg cttcaatata tcccacaaac
     7681 atttagacat tatgctgtta gaggtcacaa caatatatat agtggttacg atcagttatt
     7741 agcgttcttt aacaacagat attggcgctc acagtttaac ccaagaggtg gttggtctcc
     7801 aagtggtcca agaagatatg cgaatggtgg tttgattaca aagcatcaac ttgctgaagt
     7861 gggtgaagga gataaacagg agatggttat ccctttaact agacgtaaac gagcaattca
     7921 attaactgaa caggttatgc gcatcatcgg tatggatggc aagccaaata acatcactgt
     7981 aaataatgat acttcaacag ttgaaaaatt gttgaaacaa attgttatgt taagtgataa
     8041 aggaaataaa ttaacagatg cattgattca aactgtttct tctcaggata ataacttagg
     8101 ttctaatgat gcaattagag gtttagaaaa aatattgtca aaacaaagtg ggcatagagc
     8161 aaatgcaaat aattatatgg gaggtttgac taattaatgc aatcttttgt aaaaatcata
     8221 gatggttaca aggaagaagt aataacagat tttaatcagc ttatattttt agatgcaagg
     8281 gctgaaagtc caaacaccaa tgataacagt gtaactatta acggagtaga tggtatttta
     8341 ccgggcgcaa ttagttttgc gcctttttca ttagtattaa ggtttggcta tgatggtata
     8401 gatgttatag atttaaattt atttgagcat tggtttagat ctgtgtttaa tcgcagacat
     8461 ccttattatg ttattacttc tcaaatgcct ggtgttaaat atgcagtgaa tacagctaat
     8521 gttacatcta atttaaaaga tggttcttca actgaaattg aagtaagttt aaatgtttat
     8581 aaagggtatt ctgaatcagt taattggacc gatagcgagt tcttattcga ctctaattgg
     8641 atgtttgaaa atggaattcc tcttgatttc acacctaaat atactcatac atcaaatcaa
     8701 tttactattt ggaacggttc tactgatacg ataaatccac gattcaagca cgatttgaaa
     8761 atattaatta atttaaatgc gagtggagga tttgaactgg ttaactatac aacaggtgat
     8821 atttttaagt acaacaaaag tatagataaa aacactgatt ttgttttaga tggtgtgtat
     8881 gcatatcgag atataaatag agtgggaatt gatacaaata gaggcattat aacattagcg
     8941 ccaggtaaaa atgaatttaa gattaaagga gacgtcagtg atattaaaac tacatttaag
     9001 tttcctttta tttataggta ggtgatttaa tggattatca tgatcattta tcagtaatgg
     9061 attttaatga attgatttgt gaaaatttac tagatgtaga ttatggttct tttaaagaat
     9121 attatgaact gaatgaagct aggtacatca cctttacagt ttatagaact actcataata
     9181 gttttgtttt tgatttattg atttgtgaaa acttcataat ttatcatggt gaaaaatata
     9241 caattaagca gacagcgcca aaggttgaag gtgataaagt ttttattgaa gttacggcat
     9301 atcacataat gtatgaattt caaaatcact cagtggaatc aaataagctt gatgacgaca
     9361 gtagcgaaac tggtaaaacg ccagaatact ctttagatga gtacttaaga tatggatttg
     9421 caaatcaaaa aacttcggtc aaaatgacct ataaaataat tggagatttt aagcgaaaag
     9481 taccgattga cgaattaggt aacaaaaacg gcttagaata ctgtaaagaa gcggtagacc
     9541 tgtttggctg tataatttac ccaaatgata cagagattgg tttttattct cctgaaacat
     9601 tttatcaaag aagcgagaaa gtgattcgat atcaatataa tactgatact gtatctgcaa
     9661 ctgtcagtac attggaatta agaacagcta taaaagtttt tggaaaaaag tatacagctg
     9721 aggaaaagaa aaattataat cctattagaa caactgacat taaatattca aatggtttta
     9781 taaaagaagg tacttatcgt accgaaacaa ttgggtctaa agctactatt aactttgatt
     9841 gcaagtatgg taatgaaaca gttagattta caataaaaaa gggctctcaa ggtggaatat
     9901 ataagttgat tttagacggc aagcaaatta agcaaatttc ttgttttgct aagtcggttc
     9961 agtctgaaac aatagattta ataaaaaata ttgataaagg caagcacgtt ttagaaatga
    10021 tatttttagg agaagacccc aaaaatagaa ttgatatatc ttcaaataaa aaagctaagc
    10081 cttgtatgta tgttggaact gaaaaatcaa cagtcttaaa tttaattgct gataattcag
    10141 gtcgcaatca atacaaagca attgtcgact acgtcgcaga tagtgcaaag cagtttggga
    10201 ttcgatatgc taatacgcaa acaaatgaag atatcgaaac acaggataag ctgttagaat
    10261 ttgcaaaaaa gcaaataaat gatactccta agactgaatt agatgttaat tatataggtt
    10321 atgaaaaaat agagccaaga gatagcgtat tttttgttca tgaattaatg ggatataaca
    10381 ctgaattaaa ggttgttaaa cttgataggt cacatccatt tgtaaacgca atagatgaag
    10441 tgtctttcag caatgaaata aaagatatgg tacaaattca acaagcactt aacagacgag
    10501 ttattgcaca agataataga tataactatc aagcaaatcg tataaatcat ttatacacta
    10561 gtactttgaa ttctcctttc gagacaatgg atatagggag tgtattaata taatggcaac
    10621 agaagaagtt aaaatcaaag cgctacttga aaacgataaa cagtactttc cagctacaca
    10681 ctggaaagct ataaatggga taccttatgc aggcagtagt gatattgatg gattgcctca
    10741 agacggtatc atttcggtag atgataaaaa taaattagat aaattaaaaa taggcgaagc
    10801 aggaattatt caaaatagca ttgtacagaa atccccaaac ggtaaattgt ggaaaataac
    10861 agttgacgat agtgggaaac ttggtacagt gctattttat tagaaaggaa ggtgcattat
    10921 ggaaaatttg tatttaataa aggatttggg agctttagca ggtcgagatt atagagctaa
    10981 agaaattcaa aacctgcaaa gaatagagca atttgcgctt ggcttgacaa cagagtttaa
    11041 gttgcatcag aaagctaaaa caatgcaaca cttcgctgag caaatttatt ataatggtag
    11101 atcgcaagca gcagtaaaca aatctttaca aagtcaaatt aacgcacttg ttgtggcacc
    11161 acgtaataac agtgctaatg agattgttca agctcgagtt aatgtaaacg gcgaaacctt
    11221 tgacacatta aaagaacatt tagacgattg ggaaaccaaa actcaaatta ataaagagga
    11281 aactataaga gaattaaata agaccaaaca agaaattctt gatatcgagt atcgttttga
    11341 acctgataag caagagtttt tatttgtgac agaacttgca cctcttacaa atgcagtaat
    11401 gcaatccttc tggtttgata atagaacagg catagtatac atgacacaag ctagaaataa
    11461 tggctatatg ctaagtcgtt taagacctaa tggtcaattt atagacagct cattgattgt
    11521 aggtgggggt catggtacac ataacggtta tagatatatt gatgatgagt tatggattta
    11581 tagttttatc ttaaatggta ataatgagaa tacattagtt cgtttcaagt atacgcctaa
    11641 tgtggaaatt agctatggca agtatggtat gcaagatgta tttacaggac acccagaaaa
    11701 accctacatc acccctgtca taaatgaaaa agaaaataaa attctataca gaattgagag
    11761 acctagaagt cagtgggaac ttgaaaactc aatgaattat atagagataa gaagtttaga
    11821 cgatgttgat aaaaatattg ataaagtttt gcataaaatc agtatcccta tgagactaac
    11881 aaacgaaacc caaccaatgc agggtgtgac ttttgatgaa aaatacttgt attggtatac
    11941 aggagacagt aatccaaata atagaaacta tttaacggct ttcgatttag aaacaggaga
    12001 agaagcgtat caggttaatg ctgactatgg tggaacacta gattcatttc ctggcgaatt
    12061 tgcggaagca gaaggtttgc aaatatacta tgacaaagat agtggtaaaa aagctttgat
    12121 gctaggtgtt actgtcggtg gtgatggaaa tagaacacat cgtattttca tgattgggca
    12181 aagaggtatt ttagaaatac ttcactcaag aggcgttcct tttatcatga gtgacacagg
    12241 tggtagagtt aaacctttac caatgaggcc tgataaactt aagaatcttg ggatgttaac
    12301 agagccaggt ctttactatt tatacactga tcatacagtt caaatcgatg atttcccatt
    12361 accaagagaa tggcgtgatg caggttggtt cttggaagtt aagccaccac aaactggcgg
    12421 tgatgtaatt cagatattga cgcgtaatag ttatgcaagg aatatgatga cttttgaaag
    12481 ggtgctttct ggaagaactg gagacatttc ggactggaat tatgtgccta aaaatagtgg
    12541 taaatgggag agagtacctt cattcatcac aaaaatgtca gatattaaca tagtaggcat
    12601 gtcgttttat ttaactacgg atgatacaaa acgttttaca gattttccaa ctgaacgtaa
    12661 aggggtagct ggttggaact tatatgtaga agcttcaaac acaggtggct ttgttcatag
    12721 gctagttcgt aatagtgtta cagcatctgc tgagatacta ttgaaaaatt atgatagtaa
    12781 aacaagttca gggccatgga ctttacacga agggagaatt ataagttaat gagtaattta
    12841 gagaaatctg tagctataaa tttagaaaac acagcgcatt atgaaaatat ttcaaatcta
    12901 gatataactt ttagaacagg agagagtgat tcttctgttc ttctttttaa tatcattaaa
    12961 aataatcaac cgttattact gagtgaagaa aatatcaaag cacgaatagc gattcgaggt
    13021 aaaggagtaa tggtagttgc tccactagaa atattagatc catttaaagg tattttaaaa
    13081 tttcaattac ctaatgatgt aattaaaaga gatggaagtt atcaagctca agtttcggtt
    13141 gcagaattag gtaattcaga cgtggtagtt gtagagagaa ctatcacatt taacgttgaa
    13201 aaaagtttgt ttagcaagat tccctctgaa acaaaactac actatattgt tgagtttcaa
    13261 gaattagaaa aaactattat ggatcgcgcg aaagcaatgg acgaggctat aaaaaatggt
    13321 gaggattatg cgagtctgat tgaaaaagct aaagaaaaag gtctatcaga tattcaaata
    13381 gcaaaatctt caagtattga tgaattaaag caacttgcta atagccgtat atctgatttg
    13441 gaaaataaag cgcaagcata ttcaagaaca ttcgatgagc aaaagcgata tatggatgag
    13501 aaacatgaag ccttcaagca gtcagtgaat agtggtggtt tagtcacaag tggttctact
    13561 tcaaattggc aaaaagctaa gattactaaa gatgatggta agataatgca gattactgga
    13621 tttgatttta ataatccaga acaaagaata ggtgattcaa cccaatttat ttatgtttcg
    13681 caagctataa attatccaag agatgttagt actaacggta ctgtcgaata tttagtagta
    13741 acttcagatt acaagcgtat gacttatcga ccgaacggta caaataaagt gtttgttaaa
    13801 agaaaagaag cgggttcatg gtctgagtgg tcagaattag ctattaatga ttacaataca
    13861 ccttttgaaa ctgttcaaag tgcccaatca aaagctaata tggccgaaag taacgctaaa
    13921 ttatacgcag atgacaagtt taataaaagg tattcggtta tttttgatgg aacagcaaat
    13981 ggtgtgggct ctacattgta cttaaatgag agtttagacc aatttatttt attaattttt
    14041 tatgggactt ttccaggtgg tgactttaca gagtttggca gtccttttgg aggaggaaag
    14101 atttcattga atccctcaaa tcttccagat ggtgatggaa atggtggagg tgtttatgag
    14161 tttggattaa ctaaatctag tcgtacatct ttaactatat caaacgatgt ctatttcgac
    14221 ttaggaagtc aaagaggctc tggtgcgaac gcaaatagag ggacaattaa caaaattata
    14281 ggagtgagaa aataatgcaa atattagtta acaagcgtaa tgagataatt tcatacgcta
    14341 tcattggcgg ctttgaagaa ggtattgata ttgaaaattt accagaaaat ttctctcaag
    14401 tttttagacc taaagccttt aaatattcaa atggggaaat agtttttaac gaagattatt
    14461 cagaagaaaa agatgacttg catcaacaga ttgacagtga agaacaaaac acagtcgctt
    14521 ctgatgacat cttacgaaaa atggttgcta gtatgcagaa acaagttgtt caaagtacaa
    14581 agttatcgat gcaagttaat aagcaaaatg cactaatggc aaaacaactt gtgacactta
    14641 ataaaaaatt agaagaggtt aaaggagaga ctgaaaatgc ttaaattaat ttcaccaaca
    14701 ttcgaagata ttaaaacatg gtatcaattg aaagaatata gtaaagaaga tatagcgtgg
    14761 tatgtagata tggaagttat agataaagag gaatatgcaa ttattacagg agaaaagtat
    14821 ccagaaaatc tagagtcata ggttataatc ttatggcttt ttaatttgaa taaagtgggt
    14881 ggtgtaatgt ttggatttac caaacgacac gaacaagatt ggcgtttaac gcgattagaa
    14941 gaaaatgata agactatgtt tgaaaaattc gacagaatag aagacagtct gagaacgcaa
    15001 gaaaaaattt atgacaagtt agatagaaat ttcgaagaac taaggcgtga caaagaagaa
    15061 gatgaaaaaa ataaagagaa aaatgctaaa aatattagag acatcaagat gtggattcta
    15121 ggattaatag ggacgattct aagtacattt gttatagcct tgttaaaaac tatttttggc
    15181 atttaaagga ggtgatcacc atgcttaagg gaattttagg atatagcttt tggtcgtgtt
    15241 tctggtttgg taagtgtaag taatatttaa gagtcagtgc ttcggcactg gctttttatt
    15301 ttggataaaa ggagcaaaca aatggatgca aaagtaataa caagatacat cgtattgatc
    15361 ttagcattag taaatcaatt cttagcgaat aaaggtataa gtccgatacc agtagatgaa
    15421 gaaagtgttt catcgattat cttaacagtt gttgctttat atactacata taaagataat
    15481 ccaacatctc aagaagggaa atgggcgaat caaaaattaa agaaatataa agctgaaagt
    15541 aaatatagaa aagcaacagg gcaagcgcca attaaagaag taatgacacc tacgaatatg
    15601 aacgacacaa atgatttagg gtaggtgttg accaatgttg ataacaaaaa accaagcaga
    15661 aaaatggttt gataattcat tagggaagca gttcaatcct gatttgtttt atggatttca
    15721 gtgttatgat tacgccaata tgttctttat gttagcgaca ggcgaaaggc tgcaaggttt
    15781 atatgcttat aatatcccgt ttgataataa agcaaagatt gaaaaatatg gtcaaataat
    15841 taaaaactat gacagctttt taccgcaaaa gttggatatt gtcgttttcc cgtcaaagta
    15901 tggtggcgga gctggacacg ttgaaattgt tgagagcgca aatttaaaca ctttcacatc
    15961 atttggtcaa aactggaacg gtaaaggttg gactaatggc gttgcgcaac ctggttgggg
    16021 tcctgaaact gtgacaagac atgttcatta ttatgacaat ccaatgtatt ttattaggtt
    16081 aaacttccct aacaacttaa gcgttggcaa taaagctaaa ggtattatta agcaagcgac
    16141 tacaaaaaaa gaggcagtaa ttaaacctaa aaaaattatg cttgtagccg gtcatggtta
    16201 taacgatcct ggagcagtag gaaacggaac aaacgaacgc gattttatac gtaaatatat
    16261 aacgcctaat atcgctaagt atttaagaca tgcaggacat gaagttgcat tatacggtgg
    16321 ctcaagtcaa tcacaagata tgtatcaaga tactgcatac ggtgttaatg taggcaataa
    16381 aaaagattat ggcttatatt gggttaaatc acaggggtat gacattgttc tagaaataca
    16441 tttagacgca gcaggagaaa gcgcaagtgg tgggcatgtt attatctcaa gtcaattcaa
    16501 tgcagatact attgataaaa gtatacaaga tgttattaaa aataatttag gacaaataag
    16561 aggtgtaaca cctcgtaacg atttactaaa tgttaatgta tcagcagaaa taaatatcaa
    16621 ttatcgctta tctgaattag gttttattac taataaaaat gatatggatt ggattaagaa
    16681 aaattacgac ttgtactcta aactaatagc tggtgcgatt catggtaagc caattggtgg
    16741 agtggtagct agtgaggtca aagcgccagt taaaaacgaa aagaatccgc cagtgccagc
    16801 aggttataca ctcgataaga ataatgtccc ttataaaaaa gaacaaggca attacacagt
    16861 agctaatgtt aaaggtaata atgtaagaga cggttattca actaattcaa gaattacagg
    16921 ggtattaccc aacaacacaa caattacgta tgacggtgca tattgtatta atggttatag
    16981 atggattact tatattgcta atagtggaca acgtcgctat attgcgaccg gagaggtaga
    17041 catagcaggc aaccgaataa gcagttttgg taagtttagt gcagtttgat aattgtatat
    17101 gatgaatctt aggcaggtac ttcggtactt gcctattatt taaaattaat aaacagttaa
    17161 tttttacatg aatatattaa attttaaaaa aacaaacgtt tttagtatat aaattatttt
    17221 gtgttcgtat tgtgtgctat gattaaaaag ttgttatggt caactatatc gtggttttat
    17281 gtttattatc aatcaaaata taaattattt ataatttgtt tggtaatgaa cgggtttttt
    17341 tcgaaataat a
//
LOCUS       DANJFZ010000052        17318 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.052, whole genome shotgun sequence.
ACCESSION   DANJFZ010000052 DANJFZ010000000
VERSION     DANJFZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.052"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..305
                     /locus_tag="PWN45_001512"
     CDS             <1..305
                     /locus_tag="PWN45_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0623281.1"
                     /translation="YVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLND
                     EPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            529..1635
                     /gene="rpoD"
                     /locus_tag="PWN45_001513"
     CDS             529..1635
                     /gene="rpoD"
                     /locus_tag="PWN45_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL0623282.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1766..2443
                     /locus_tag="PWN45_001514"
     CDS             1766..2443
                     /locus_tag="PWN45_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL0623283.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            2446..3546
                     /locus_tag="PWN45_001515"
     CDS             2446..3546
                     /locus_tag="PWN45_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL0623284.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            3660..5006
                     /locus_tag="PWN45_001516"
     CDS             3660..5006
                     /locus_tag="PWN45_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0623285.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            5016..5906
                     /locus_tag="PWN45_001517"
     CDS             5016..5906
                     /locus_tag="PWN45_001517"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL0623286.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            6032..6817
                     /locus_tag="PWN45_001518"
     CDS             6032..6817
                     /locus_tag="PWN45_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0623287.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            6859..7722
                     /locus_tag="PWN45_001519"
     CDS             6859..7722
                     /locus_tag="PWN45_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0623288.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            7709..8119
                     /locus_tag="PWN45_001520"
     CDS             7709..8119
                     /locus_tag="PWN45_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0623289.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            8396..8995
                     /locus_tag="PWN45_001521"
     CDS             8396..8995
                     /locus_tag="PWN45_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL0623290.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            9116..11191
                     /locus_tag="PWN45_001522"
     CDS             9116..11191
                     /locus_tag="PWN45_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL0623291.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            11304..11453
                     /gene="rpmG"
                     /locus_tag="PWN45_001523"
     CDS             11304..11453
                     /gene="rpmG"
                     /locus_tag="PWN45_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0623292.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            11662..12201
                     /locus_tag="PWN45_001524"
     CDS             11662..12201
                     /locus_tag="PWN45_001524"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL0623293.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            12213..13676
                     /locus_tag="PWN45_001525"
     CDS             12213..13676
                     /locus_tag="PWN45_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL0623294.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            13657..13860
                     /locus_tag="PWN45_001526"
     CDS             13657..13860
                     /locus_tag="PWN45_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL0623295.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            13857..14843
                     /locus_tag="PWN45_001527"
     CDS             13857..14843
                     /locus_tag="PWN45_001527"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL0623296.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            14843..15172
                     /locus_tag="PWN45_001528"
     CDS             14843..15172
                     /locus_tag="PWN45_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDL0623297.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            15169..15792
                     /locus_tag="PWN45_001529"
     CDS             15169..15792
                     /locus_tag="PWN45_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0623298.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            15844..16818
                     /locus_tag="PWN45_001530"
     CDS             15844..16818
                     /locus_tag="PWN45_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDL0623299.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            16790..>17318
                     /locus_tag="PWN45_001531"
     CDS             16790..>17318
                     /locus_tag="PWN45_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL0623300.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTI"
BASE COUNT         6462 a         2258 c         3160 g         5438 t
ORIGIN      
        1 aatatgtatt cgaagtctta catgattttt atgcggaaaa tgatcaatat aatatcagtg
       61 atgctgtgca gtatgttaat tcaaatgagt tgagagaaac actaattagc ttagaacaat
      121 ataatttgaa tgacgaacca tatgaaaatg aaattgatga ttatgtcaat gttattaatg
      181 aaaaaggaca agaaacaatt gagtcattga atcataaatt aagggaagct acaaggattg
      241 gcgatgtaga attacaaaaa tactatttac agcaaattgt tgctaagaat aaagaacgca
      301 tgtagcatgt gattttaaag aataatacga ataatgatta tgtcaaaatg tataagggta
      361 aatgatagtt accgcattta aacaacacta ttgaaaaata aatattggga ttagttccaa
      421 tttgtaaaat aaaattaaaa atatggatga attaattaag aatttagttt aaaatagcaa
      481 tattgaataa atttcgaatg ttcatattta aaatcgggag gccgtttcat gtctgataac
      541 acagttaaaa ttaaaaaaca aacaattgat ccgacattaa cattagaaga tgttaagaag
      601 caattaattg aaaaaggtaa aaaagagggt catttaagtc atgaagaaat tgctgaaaaa
      661 cttcagaatt ttgatatcga ctctgatcaa atggatgatt tctttgatca attaaatgat
      721 aatgatattt cactagttaa tgaaaaagat agttcagata ctgacgagaa actgaatcca
      781 agtgatctta gtgcccctcc aggtgttaaa ataaatgacc cagttcgtat gtaccttaaa
      841 gaaattgggc gtgttaactt attaagtgca caagaagaaa tcgaattagc caaacgtatt
      901 gaacaaggtg atgaagtagc aaaatcaaga cttgcagaag cgaacttacg tttagttgta
      961 agtattgcta aaagatacgt aggtcgtggt atgttattcc ttgatttaat ccaagaaggt
     1021 aatatgggtc ttattaaagc tgttgaaaaa tttgacttta acaaaggatt taagttttca
     1081 acatatgcaa catggtggat tagacaagca atcactcgtg caattgctga ccaagcacgt
     1141 acgattcgta tccctgtgca tatggtagaa acaattaata aattaattcg tgttcaacgt
     1201 caattattac aggacttagg tcgagatcca gcaccagaag aaattggtga agaaatggat
     1261 ttaccagcag aaaaagttcg tgaaatttta aaaattgcgc aagaacctgt ttcattagaa
     1321 acaccaattg gtgaagaaga tgatagtcat ttaggagact ttattgagga tcaggaagca
     1381 caaagtcctt cagatcatgc tgcttatgaa ttattaaaag agcaattaga agatgtgctt
     1441 gatacattaa ctgatagaga agaaaatgta ttacgattaa gatttggtct tgatgacggc
     1501 agaacaagaa cacttgaaga agttggtaaa gttttcggtg ttacacgtga acgtattcga
     1561 caaattgaag caaaagcact tagaaaatta agacatccaa gtcgtagtaa acgtttgaaa
     1621 gactttatgg attaatttat atcaatttta atgattgata ccaagacatg aataagcgct
     1681 tatttatttt tagattaaga tttaacttga gtgaggttgg tacattgaat aatgttcaac
     1741 ctcttttttc ataaaggaga ataaaatgat ttcgttaaat aaccgattaa cgacggtaag
     1801 tcgattttta aaacaaggta caatcgcgga tattggctca gatcatgctt atttgccaat
     1861 ctatgcaatt caaaaccatt tatgcgaatg cggtattgct ggagaagtta ttcaaggccc
     1921 ttttcaagct gctgtgaaaa atgtagctgc aaaccaatta gtagatagaa ttgatgtacg
     1981 tttaggggat ggcttaagtg ttattcaacc tgaggacgta atcgataata tcacaatttg
     2041 tggtatgggc ggaccactta ttgctaaaat tttaaaagat gggcaagata agttaagtca
     2101 acatccaagg ttgattttac aaagtaatat ccaaactgaa aatttaagac aaacattaca
     2161 acaattgaat tatgaaataa ttgatgaaat aataatggaa gaaaaaggcc atatttatga
     2221 aattgttgta gcagaatatt ctacagaatt aatagagcta tcatctgatg aattgaaatt
     2281 cggaccaaaa ttgctgaaca acaaaaatga atattttata aaaaaatggc aaagagagtt
     2341 agaagcactt tatcatatta aatcgaaact caatactgaa caacatcatc aacgtttagc
     2401 tcaaattaat gacgaaattg ctgtaattga aagggtgtta tagtaatgaa aatagctgat
     2461 ttaatgacat tgttagatca tcatgtgcca tttagtactg ctgaatcttg ggataatgta
     2521 ggattgttaa taggtgatga agatgttgaa gttactggtg ttttaacagc attagactgt
     2581 acgttggaag tagtaaatga agcaatcgaa aaaggttata atactattat tagtcatcat
     2641 cctctaatct ttaaaggcgt aacatcatta aaagctaatg gttatggttt gatcattaga
     2701 aaactaattc aacatgacat taatttaata gcgatgcata caaatttaga tgtaaatccg
     2761 tatggtgtca atatgatgtt ggcgaaggcg atgggtttga agaacatttc aataataaat
     2821 aatcaacaag atgtatacta taaagttcaa acatatatac ctaaggataa tgttggacca
     2881 tttaaagata agcttagtga aaatggatta gcgcaagaag gtaattatga atattgtttc
     2941 tttgaaagtg aaggaagagg gcaattcaaa ccagttggtg aagctaatcc aacaatagga
     3001 caaattgata aaattgaata tgtagatgaa gttaaaattg aatttatgat agatgcatat
     3061 caaaagtcaa gggctgagca attaattaaa caataccatc catatgaaac accggtattt
     3121 gattttattg agataaaaca aacatccctt tatggacttg gcgttatggc agaagtggat
     3181 aatcaaatga cattggaaga tttcgcagct gatattaaat ctaaattaaa tatcccaagt
     3241 gtccgttttg ttggtgagtc taatcagaaa attaaacgta ttgcaattat tggtggttca
     3301 ggtattggat atgaatatca agctgtccaa caaggcgcag atgtctttgt tacaggtgat
     3361 attaaacatc atgatgcctt agatgctaaa attcatggtg tgaatttaat tgatattaat
     3421 cattacagcg aatatgtgat gaaagaaggt ttaaaagcgt tactaatgaa ttggttcaat
     3481 acagaaaaaa taaatttaga tgttgaagca tctacaatta atacagatcc atttcaatat
     3541 atttaaacta aaatctcggg atttctaaat tttgaaattt cgaggtttta atatttttat
     3601 ttaaaatagc acatttatac tttataatag taaagatgaa catataagga ggccaaatca
     3661 tggcaaaaca tccattcgaa caatttaatc tagaatctag tttaattgac gctgtgaaag
     3721 accttaattt tgaaaaacca actgaaattc agaatcgaat tattccaaga atactaaaga
     3781 gaacaaattt aattggtcaa tctcaaacgg gtacagggaa atctcatgca tttttattac
     3841 cattaatgca gttaattgat agtgaaataa aagaaccaca agcaatcgta gttgcaccaa
     3901 caagagaact tgcacaacaa ctatacgatg cagcgaacca tttaagccaa tttaaagctg
     3961 gtgtttcagt taaagttttt attggtggta cagatataga gaaagataga caacgttgta
     4021 atgcacaacc acaattgatt ataggcaccc ctactagaat taatgactta gctaaaacgg
     4081 gacatttaca tgtgcactta gcatcatatt tagttattga tgaagcggat cttatgattg
     4141 acttaggatt aattgaagat gtagattaca ttgctgcaag attggaagat aatgcaaata
     4201 ttgcggtgtt tagtgctaca atcccacaac agttacaacc atttttaaat aaatatttaa
     4261 gtcatccaga atatgtagct gtcgacagta aaaaacaaaa taaaaagaac atcgaattct
     4321 atttaatacc tactaaaggt gcagctaaag ttgaaaagac tttaaattta attgatatac
     4381 taaatccata cttatgtatt attttctgta atagtagaga taatgcaaat gatttagcac
     4441 gttcactaaa tgaagctggt attaaagttg gtatgattca tggtggctta acgccacgtg
     4501 aacgtaaaca acaaatgaaa cgtatacgta atttagaatt ccaatacgtt attgccagcg
     4561 atttagcatc tcgtggtatt gatattgaag gtgttagtca tgtcatcaat tttgatgtgc
     4621 caaatgatat tgacttcttt acgcatagag tcggacgaac tggtcgtggg aattataaag
     4681 gtgtagcaat tacgctttat agtcctgatg aagaacacaa tatttcatta atagaagatc
     4741 gcggttttgt attcaatact gttgatatta aagatggtga gttaaaagaa gttaaagcgc
     4801 acaatcagcg tcaagcaaga atgcgcaaag atgaccattt aactaatcaa gtgaagaaca
     4861 aagttcgaag taaaattaaa aacaaagtta aaccaggtta taagaagaaa tttaaacaag
     4921 aagttgaaaa aatgaaacgt caagagcgta agcaatttag taagcagcaa aatagacaaa
     4981 aacgtaagca aaacaaaaaa ggttaggtgt gttttatgtt attaggatca catgtttcaa
     5041 tgagtggtaa aaagatgtta gaaggttctg ctatagaagc gcatgaatat ggtgaaacaa
     5101 catttatgat ttatactggt gcacctcaaa acacgcgccg taaaagtatt gaagatttaa
     5161 atataactaa aggtcatgaa gtgatggaaa aatatggttt atctaatatt gttgttcatg
     5221 caccatacat cattaatatt gcaaatacaa ccaaaccaga aacgtttaat cttggtgtag
     5281 atttcttgca acaagaaatt gaaagaacgc aagctatagg ggctaaagat attgtattac
     5341 atccaggtgc gcatgttgga gctggtgttg atgccggaat taataagatt attgaaggat
     5401 taaatgaagt tttaacgaat gacaataatg tacgtattgc gcttgaaacg atggcaggta
     5461 aaggtacaga gattggtcgc tcatttgaag agttagcacg tattattgat ggagttcata
     5521 ataacgaacg tttatcagta tgttttgata catgtcatac gcatgatgct ggatataatg
     5581 ttaaagagga ctttgatggc gtattaaatg aatttgataa aatcattgga gtcgacagaa
     5641 tcaaagttgt tcatgtcaat gattctaaaa atgaccgtgg cgctcaaaaa gaccgacatg
     5701 aaaatattgg cttcggttac attggttttg atgcattaaa ttacattgtg catcatgata
     5761 gttttaaaga tattccgaaa atcttagaaa caccttatgt tggtgaagat aagaaaaata
     5821 aaaaaccgcc atataaactt gaaattgaaa tgttaaaaca acagcaattt gacccagaat
     5881 taaaaaataa ggttatgcaa caataaaatg ataatgctct cttcaagaaa tgcgataaat
     5941 aaaacttgaa gggggcatat ctctttattt tgtctaattt tgagtcgtaa acattactgt
     6001 ttacatatgt tactaggagt tgtatagttg aatgcgaggt gagaatacga tgacaccagt
     6061 ctttgaattg aaaaatgtca attactacta tgatcataaa aaagtgttag aaaatataaa
     6121 cattaaaata aataaaggtg aatttttagc aattgttgga ccaaatggtg ctggtaaatc
     6181 aacattattg aagttgattc tagggttatt acctttacaa agtggtgaga tttttgttga
     6241 aggtattgat tttaaaaata agaaaacatc aattaaatta agctatgtat cacaaaaagc
     6301 aaatgccttt aattcaggtt tcccagcaag tgttaaagaa gttgttttaa gcggattaac
     6361 aaagacaaaa cgtcttttcc aaacatttaa tagcaaagat aatgaaaaag tgattaaagt
     6421 actagaaaga ctgaatataa gtgatttaat tcataaaaat atagcagaat tatcaggtgg
     6481 tcaacaacaa cgtgtaatga ttgctcgagc attgatttca gaacctgcag tattagtact
     6541 tgatgaacca acgaatggta ttgatgcaaa acatgtaagt gaattttata atactttaga
     6601 tcaattaaaa caagaaggta tcaccattat cttagttact catgatatcg gtgttgtagc
     6661 agatactgct actgaagtag catgtttaaa taagcatttg catttccatg gtacaactga
     6721 tgagtttaaa tcacttgatg aagttgaaat ttcaaaaatt tatggacatc ctgtacgttt
     6781 tgtcgatcat cagcataatc gagaatgttg taattaataa agatttgagt ttatttaaaa
     6841 tggaaaggcg tgtcacaatt gatagatgca ttattgaatt ttgattttat gaggtactct
     6901 ttaattagtg gtatcttgat aggttttatt gcgcctctaa tcggtgcttt tatcgttgtt
     6961 agacgactat cacttatagc tgatgctcta agtcatgtaa ctttaggtgg tatatctttc
     7021 ggtatgtttt tacttactat tatgccaaca ctagtattta ttaatccaat gtggtttgga
     7081 atcttattcg caatagtagg tgcgcttcta attgaaaaat taagaacgtc atacactgct
     7141 taccaagaaa ttgctattcc aattataatg agtgctggta tcgccttgag tgcaatcttc
     7201 atttcattag ctgatgggtt caatcaagaa attgttggat tgctatttgg ctcaataagc
     7261 gcagtgaata taagtgattt aacaacgatt attgtcattg ccatcatagt tgttctgttt
     7321 attacattgt tttataaaga attatttata ttgtcatttg atgaggaata tagtaaagtc
     7381 attggcattc ctaaatggat tcaattttta tttattgtta ttgtagcgat ggttatttct
     7441 gcctcaatgc gtgttgtagg gatactgtta gtaagtgcgt taatcacgtt accaattgca
     7501 ataagtatgc gaataacaaa aagttttaag caattgatat tactaagtgt atttttaggt
     7561 gaattatcag taatattagg cttagtccta gctttctata tggacatttc tccgggtggt
     7621 gtaattgtag ttctacttgt tattttactt atgattacaa tggcttatca gaaaatgcga
     7681 atgaagttta aaaagggagc taatatcaat gaatacaaat gatgctatta aaattttaaa
     7741 agagaacggt ttaaaatata cagataaacg taaagatatg ttagatattt ttgtcgaaga
     7801 agataagtat ataaacgcaa agtatataca acaagttatg gatgaaaatt atcctggaat
     7861 ttcattcgac acaatatata gaaacctgca cttatttaaa gatttaggaa ttattgaaaa
     7921 tacagaactt gatggtgaaa tgaagtttag aatcgcttgt acaaaccatc atcatcatca
     7981 ttttatctgt gaaaagtgtg gagatacaaa ggtaatagat tattgtccaa tagatcagat
     8041 aaaattatca ctacctggtg ttaatattca caaacacaaa cttgaagttt atggtgtatg
     8101 tgagtcttgc caagattaat ataaagaaat gagatttatg cacatttggt cagatgtatg
     8161 cataaatctc atttctttat attatagaag ataaaggttc atttcatact ccgttcgtat
     8221 gattttatgg caaatctgtt aactttttat atacaaaatg tttaattatt tttgatttga
     8281 gttatattaa atgagtagag aaaataataa gtagtagaat ttaagcaaat tctttgttga
     8341 accagtcact gcttgttatt atttacttac agacatttta ggaggatgat tatttatggc
     8401 ttttgaatta ccaaaattac catacgcatt tgatgcatta gaaccacatt ttgacaaaga
     8461 aactatggaa attcaccatg acagacatca taacacgtat gttacgaaat taaatgctgc
     8521 agtagaaggt acagatttag aatctaaatc tattgaagaa attgttgcta atttagacag
     8581 tgtaccagct aacatccaaa ctgctgtacg taataatggc ggtggacatt taaaccattc
     8641 attattctgg gagttacttt caccaaactc agaagaaaaa ggtactgtag tagaaaaaat
     8701 taaagaacaa tggggttctt tagaagaatt taaaaaagaa tttgctgaca aagcagctgc
     8761 acgctttggt tcaggttggg cttggttagt cgtaaacaat ggccagttag aaattgtgac
     8821 tacaccaaac caagataatc cattaactga gggtaaaaca cctattttag gtttagacgt
     8881 atgggaacac gcttattacc taaaatatca aaacaaacgc cctgactaca ttggcgcatt
     8941 ttggaatgta gttaactggg aaaaagttga cgaattatat aatgcaacaa aataaagtat
     9001 gattacttgt tcggtctctt aaatgagacc gttatttttt tgtcaaaaag atagaaataa
     9061 tttctaaatt catatatgat ttaaagtgaa agactttgaa tagaggtagg tagttttgtt
     9121 aaaaagacta aaagaaaaat caaatgatga aatcgttcaa aatacaatta acaagagaat
     9181 taactttata tttggtgtga ttatatttat ttttgcagta ctagtactac gtttaggtta
     9241 tttacaaatc gcacaaggct cacattataa acaaattata aaaaatgatg aaaacattac
     9301 agtgaatgag tctgtgccaa gaggtcgtat tttagacaga aatgggaaag ttttagttga
     9361 taatgcttct aaaatggcta ttacatatac taggggtcga aaaacaacac aatcggaaat
     9421 gttggatacg gctgaaaagt tatcaaagct aatcaagatg gatactaaga aaattacaga
     9481 acgtgataag aaagatttct ggattcagtt gcatcctaaa aaagcaaaag caatgatgac
     9541 aaaagaacaa gctatgttag cagatggaag tattaaacaa gatcaatatg ataaacaact
     9601 gttatcgaaa atcggaaaat cacaattaga tgaattgtct tctaaagatt tacaagtttt
     9661 agctattttt cgagagatga atgcaggaac agttttagat ccacaaatga taaaaaatga
     9721 agatgtcagt gaaaaagagt atgcagcagt ttctcagcaa ctttccaaat taccaggtgt
     9781 taacacgtct atggattggg atagaaaata tccatatggc gatactttaa gaggtatatt
     9841 cggagatgta tcgacacctg ctgaaggtat tccaaaagaa ttgacagaac attacttatc
     9901 caaaggatat tcacgcaatg atcgtgttgg aaaatcttac ctagaatatc aatatgaaga
     9961 tgtattgcgt ggtaagaaga aagaaatgaa atacacaacg gacaaatctg gtaaagttac
    10021 atcttcagaa gtgttaaatc ctggcgctcg cggtcaagat ttgaaattaa cgatcgatat
    10081 agatcttcaa aaagaagtag aagcattatt agataaacaa attaagaagc ttcgcagtca
    10141 aggtgccaaa gatatggata atgcaatgat ggttgtacaa aatcctaaaa atggagacat
    10201 tcttgcgctt gccggaaagc agattaataa gagtggtaaa atgactgatt atgacattgg
    10261 tacgtttact tctcaatttg cggttggatc ttctgtaaaa ggtggaacat tattagccgg
    10321 ttatcagaat aaagctatca aagttggaga aacaatggtc gatgaaccat tacatttcca
    10381 aggtggtttg acaaaacgat catacttcaa taaaaacggg catgtaacta ttaatgataa
    10441 gcaagctttg atgcattcat caaacgtata tatgtttaaa acagcattaa aattagcggg
    10501 agacccttat tattctggta tggctttacc ttcagacata agttcacctg cccaaaagct
    10561 aagaagagga ttaaatcaag taggcttagg tgtgaaaaca gggatagatt taccaaatga
    10621 aacaagaggt caaatcgaac cattaacaaa taatccaggt aattatctag atttatcaat
    10681 tggtcaatat gatacctata caccattaca attatcacaa tatgtttcaa ctatagcgaa
    10741 tgatggttat agaatacagc cacacattgg attaacgatt catgaatcaa ctaataaaga
    10801 tgaggttggt ccactcaaga agaaaattaa tggcactgtc ttgaacaagg ttaataatac
    10861 tgaaaaggaa atcaaacaaa ttcaagaagg attcaaaatg gcatttaatg ataaagatgg
    10921 tactggatat gttagtttta aagatacagt agtacctact gctggtaaaa cgggtaccgc
    10981 agaagtgttc caaaacggag agccaagagt taactctact tatataggat acgcgccaat
    11041 tgatgatcca aaattagcgt tttcaattgt atatacaaat cagcctgtac caccaccatg
    11101 gttaacaggt ggagacttag gtagagatgt aattaactac tactttaagc agttaggtaa
    11161 aaatgataaa aataaagaca aagacaaata aaatttaacc tgacgattgt gtagcgcatg
    11221 gttgtaaaat tttaactttg caaatattat agatgttggt ataataataa agtcgtattt
    11281 agaaatgata aggaggaata accatgcgcg taaacgtaac tttagcttgt acggaatgtg
    11341 gtgacagaaa ctacattaca actaagaaca aaagaaataa tccagaacgt gttgaaatga
    11401 agaaattctg ttcacgtgaa aacaaacaaa ctttacaccg tgaaacaaaa taatcattaa
    11461 aattaaattt aaattaacaa taaaaaaggt gaaatctcaa aataagaggt ttcacctttt
    11521 taattatgca cacgtatgaa atgataaaag attttcaatt gaaataataa attttagaat
    11581 tgtcgcataa ttcatcatga caacataatg acatgtattg ttattaacga tataatagaa
    11641 ttgaataaag tgggtgataa tgtgactaaa aatgagatta gaaaatacat tttacataaa
    11701 atgaagaatt ttaataaagc tgaaaagcga aaagcagaca catggttaag aaatcaattt
    11761 tttgcaactg aagaatacaa agaagcaaac gcaattgcgc tagttctttc ttttaatcat
    11821 gaagtagata ctttttctat tattgaacaa gccttaatgg atcataaacg tatttttgta
    11881 ccgaaaatgg attatttaaa tcatcaaatg acttttaaag agatatttaa tctcaaagat
    11941 attgatgtcg ataataaggg gatttactat ccaacttcaa aaggtgaaac aacgaataac
    12001 ctagatttaa ttgttgttcc tggtgttgga tttcaagacg atggatatag aattgggtat
    12061 ggtggtggct attacgacag gtttttagct aattatcaga caaagacaat aagcttatta
    12121 tacgattttc aaataacatc atttgaaccg gaatcattcg atcaaccagt cgataaattg
    12181 attatatatc aagcagcata gtggaggact aaatgaacat agacaaacaa ttttggaaaa
    12241 caatatatta ttggattagg tacttgaatt ttgatattgt cagtagggaa aaagatgacc
    12301 aagaaatttg gttagcccat aaaagaaaaa aacaagtagt catttttaaa caacatatca
    12361 agtctaccca agaaatacgt tttgacaaag cgaaagtgct tgaacacaaa gatgaaatag
    12421 caaattttat ttctttcgaa ccacaaagtt ttgaatttta ttattttaca gaatcggaat
    12481 tttcagaaga acaattaaat gaagtttcgc caattagaat taaattcaat gttataagac
    12541 acacaaaaga tttgataaag catatgccga atatattttt ggctagactt atttcagaag
    12601 ataatgataa aaagacatat atgttctata aacgcaaagt attaaccgat aactttttag
    12661 ataaatatat gcagaaattt tcaccggcaa catacacaat aatatttgta aatgtcttaa
    12721 tatggttatg tatgatttta tatttaaata atttttcgga tgtaaaatta ttagatgttg
    12781 gcgggttagt gcattttaat gtcgtacatg gtgaatggta tcgaattgtt acatcgatgt
    12841 ttttacattt tagttttgaa catatactta tgaatatgct ttcattattt atttttggta
    12901 aaatagtcga agcaattatt ggttcatggc ggatgttaac tgtatacttt attgcagggt
    12961 tgtttggaaa ctttgtatca ctatcattta atacgactac aatttcagtt ggggctagtg
    13021 gtgctatatt tggtctgatt ggatcaattt ttgcgatgat gtatgtttca aaaacattta
    13081 acaaaaaaat gttaggacag ttattaattg cattagtgat attagttggt gtttctctgt
    13141 ttatgtcaaa tataaatatt gtggcgcata ttggaggatt cattggtggt ttattaataa
    13201 ctttaattgg ctattactat aaagtgaatc gtaatatttt ttggatttta ctaattggta
    13261 tgcttgttat atttattgca cttcaaatta gaatttttac aattaaagaa gataatattt
    13321 ataataaatt gatcaaagat gatatgacta gtggtaatta tgataatgct caaaatattg
    13381 tgaagcagac aataaataaa aattatgccg atgatcaaac atattattta agtggtatga
    13441 ttatggctac tatcaattct aagtccgaag gtatgacaga atgggagcga ggacttagaa
    13501 tgtttcctaa atcaggttta ttaaattttg agttagcgat agcaaatcgt tcattaaatg
    13561 atgatgaaaa agcattaaaa tatgtgcgta aagcattaaa tgcagaccct aaaaatacag
    13621 attatattaa cttagaaaaa gagttgacta aatcaaatga gtcgaaaaat aaataacttt
    13681 tatgatgtac aacagttatt gaaaagttac ggatttctaa tatattttaa aaatccagaa
    13741 gatatgtacg aaatgattca acaggagatt tcatcattgt atcaatatga actgttatct
    13801 aaggaagaat atttgaaatg tacgttgata attaatcaga gaaggaatga acagaaatga
    13861 caaaaattat tttagcagct gatgtaggcg ggacgacttg taaattaggt attttcacac
    13921 ctgaattaga acaattacat aaatggtcta ttcacactga tacatctgat agtacaggat
    13981 atacactttt gaaaggaatt tatgattcgt ttgttgaaaa agtaaatgaa aataattata
    14041 atttttcaaa tgtacttggc gtaggtattg gtgtaccagg tcctgttgac tttgaaaaag
    14101 gtacagtaaa tggagcagta aacttatatt ggccagaaaa agttaatgta cgtgagattt
    14161 ttgaacaatt cgttgattgt ccagtgtatg tagataatga tgctaacata gctgctttag
    14221 gggagaaaca caaaggtgct ggtgaaggtg ccgatgatgt tgttgccatc acacttggta
    14281 caggtctagg tggaggaatt atttcaaatg gtgaaatcgt acatggtcat aatggctctg
    14341 gcgcagaaat aggtcatttt agagcagact tcgatcaacg atttaaatgt aattgtggtc
    14401 gttctggatg tattgaaaca gttgcttcag cgacaggcgt tgttaactta gttaacttct
    14461 actatccgaa gttgacgttt agatcttcta tattagaatt gattaaagaa aataaggtta
    14521 cagcaaaagc tgtttttgat gcggcaaaag ctggtgacca attctgtatt ttcattactg
    14581 aaaaggttgc aaactatatt ggatatttat gtagtattat tagtgttaca agtaatccga
    14641 aatatatcgt tctaggtgga ggaatgtcta ctgcaggacc tattttaatt gaaaatatta
    14701 aaacagaata tcataattta acatttgcac ctgctcaatt tgaaactgaa attgtacaag
    14761 cgaaattagg taatgatgca ggtattacag gagcagcagg attaatcaag acctatgtat
    14821 tagataaaga gggggtaaaa taatggctat tgttgatgtg gttgttattc cagttggaac
    14881 ggaaggtccg agtgttagta aatatattgc agatattcag aaaaaacttc aagaatataa
    14941 agcaatgggt aaaattgatt ttcaattaac accaatgaat actctaattg aaggtgaatt
    15001 aagcgatgta ttagaagttg tgcaagtgat acatgaatta ccttttgata aaggtttaag
    15061 tagagtttgt acaaatatcc gtattgatga ccgacgagac aaatctagaa aaatgaatga
    15121 taaactaaca tcagtacaaa aacatttaga aaatagtggt gaaaacctat gaggatttca
    15181 agcttaactt taggcttagt tgatactaat acgtatttca tcgaaaatga caaagctgtt
    15241 attctgattg acccttcagg tgaaagtgaa aaaattatta aaaaattaaa ccaaataaat
    15301 aaaccgttaa aagctatttt attaacacat gcacactttg atcatatcgg agcagtcgat
    15361 gatatagttg atcgattcga tgtcccggtt tatatgcatg aagcagagtt tgattttcta
    15421 aaagatcccg ttaaaaatgg ggcagataaa tttaagcaat atggattacc aattattaca
    15481 agtaaggtaa ctcctgaaaa gttaaacgaa ggtagcacag aaatagaagg atttaagttt
    15541 aatgtgttac acacacctgg acattcacca ggaagtttaa catatgtgtt cgatgaattc
    15601 gcagttgttg gagatacatt atttaataat ggaatcggac gtacagattt atataaaggt
    15661 gattatgaaa cgctagttga ttctattcaa gataaaatat ttgaattaga aggcgattta
    15721 cctttattcc ctggacatgg tccatatacg acggttgatg atgaacaatt aaatccattt
    15781 ttacacggtt aaattgaagt aataaaaaca cctcctacgt gattatatgt aggaggtgtt
    15841 tttttgaaga ttctatttca agaaataatt aataaagcga tagaaatgaa agcgagtgat
    15901 gtacatttta ttccagttaa aaatgaagta agtattaaat ttagaattaa tgataacttg
    15961 gagcagtatg aacaaattgg gaatagcatt tatcaaaagt tattagttta tatgaagttt
    16021 caagctgggc ttgatgtttc tacacagcaa gtcgcacaga gcggtcgata tagttacctt
    16081 ttcaataaaa tatatttttt gagaatatca actttaccat tgtcacttgg ccaagaaagt
    16141 tgtgttatca gaattgtacc tcaatttttt caaccacaga aatcaactta taaattcaat
    16201 gattttaaac acctcatgaa taagaaacaa ggattactat tgtttagtgg accaactggt
    16261 tcaggaaaga gtacattaat gtatcaaatg gtctcatacg cgaataaagc cttgaattta
    16321 aatgtaattt ctatagagga tcctgtagag atgcaaattc ctggtatcgt ccaaattaat
    16381 gtgaatgata aagctggcat aaactatgta aattcgttta aagctatttt aagatgtgat
    16441 cctgatgtta ttttaatagg tgaaatcaga gataaagatg ttgccaagtg tgttatacag
    16501 gctagtttaa gtggtcacct tgttctgact acattgcatg caactgattg taaaggtgct
    16561 attttaaggc tattagaaat gggcatttct gtacaagaat tgatacaggc aactaactta
    16621 attataaacc aacgacttgt aactactatt aagcaacagc gacaattagt atgtgaaatt
    16681 ctatctcagc aacaactccg atatttcttt tcccataatc attcattacc atcatcattt
    16741 aagaacttag aagataaact tgatgatatg acaaaagcag gtgtcatttg tgaaactaca
    16801 atgcataaat acatttaaac tacattctaa gaagcgacaa ttaagtaagg cccaacaaat
    16861 cgacttactt tcaaatttat gtaatttgtt gaaatatggt ttcactctgt atcaaagttt
    16921 tcaattttta aatcttcaaa tgacatataa aaataagcaa ttaggtacca ccattctaag
    16981 tgaaatttca aatggtgcac catgcaatca aatattatca ctgataggtt atagcgatac
    17041 tatcgtcatg caagtatatt tggcagaaag atttggcaat attatagacg ttctagaaga
    17101 aaccgtaaat tatatgaaag tgaatagaaa gtcagaacaa cgattgttaa agacactgca
    17161 atacccctta atactagttt ctatctttat tgctatgatt attatattaa acctcacagt
    17221 aattccacag tttcaacaat tatatacttc tatgaatatt caactatctt cttttcaaaa
    17281 aacattgtct tttttcatta ccagcttacc tactataa
//
LOCUS       DANJFZ010000053        17226 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.053, whole genome shotgun sequence.
ACCESSION   DANJFZ010000053 DANJFZ010000000
VERSION     DANJFZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.053"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            136..1371
                     /gene="arcA"
                     /locus_tag="PWN45_001532"
     CDS             136..1371
                     /gene="arcA"
                     /locus_tag="PWN45_001532"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL0623301.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1404..2414
                     /gene="argF"
                     /locus_tag="PWN45_001533"
     CDS             1404..2414
                     /gene="argF"
                     /locus_tag="PWN45_001533"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0623302.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            2505..3935
                     /gene="arcD"
                     /locus_tag="PWN45_001534"
     CDS             2505..3935
                     /gene="arcD"
                     /locus_tag="PWN45_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL0623303.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            3952..4893
                     /gene="arcC"
                     /locus_tag="PWN45_001535"
     CDS             3952..4893
                     /gene="arcC"
                     /locus_tag="PWN45_001535"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0623304.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            4992..5696
                     /locus_tag="PWN45_001536"
     CDS             4992..5696
                     /locus_tag="PWN45_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0623305.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            6046..8745
                     /gene="clfB"
                     /locus_tag="PWN45_001537"
     CDS             6046..8745
                     /gene="clfB"
                     /locus_tag="PWN45_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDL0623306.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            8861..9622
                     /locus_tag="PWN45_001538"
     CDS             8861..9622
                     /locus_tag="PWN45_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL0623307.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            9867..10322
                     /locus_tag="PWN45_001539"
     CDS             9867..10322
                     /locus_tag="PWN45_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0623308.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(10425..10535)
                     /locus_tag="PWN45_001540"
     CDS             complement(10425..10535)
                     /locus_tag="PWN45_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623309.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(10605..12029)
                     /locus_tag="PWN45_001541"
     CDS             complement(10605..12029)
                     /locus_tag="PWN45_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDL0623310.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            12227..12427
                     /locus_tag="PWN45_001542"
     CDS             12227..12427
                     /locus_tag="PWN45_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623311.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            12453..13118
                     /gene="nsaR"
                     /locus_tag="PWN45_001543"
     CDS             12453..13118
                     /gene="nsaR"
                     /locus_tag="PWN45_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDL0623312.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            13129..14016
                     /gene="nsaS"
                     /locus_tag="PWN45_001544"
     CDS             13129..14016
                     /gene="nsaS"
                     /locus_tag="PWN45_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDL0623313.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            14124..14879
                     /locus_tag="PWN45_001545"
     CDS             14124..14879
                     /locus_tag="PWN45_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623314.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            14876..16876
                     /locus_tag="PWN45_001546"
     CDS             14876..16876
                     /locus_tag="PWN45_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623315.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         6114 a         2573 c         3160 g         5379 t
ORIGIN      
        1 aaaatccatt attatgtatt gtataacaaa aagatatgaa attttcgact ttctttatgt
       61 gaatataatc acatgtaagc gtttgaagat tgtctatact ctaaatgaat tcaaagataa
      121 aaggaggaaa tagacatgac agatggtcca attaaagtaa atagcgaaat tggagcttta
      181 aaaactgtgt tacttaagcg tcctggaaaa gaattagaaa atttagtacc tgattattta
      241 gatggattac tatttgatga tattccatat ttagaagtag ctcaaaaaga gcatgaccat
      301 tttgcgcagg tgctaagaga agagggtgtt gaagtacttt accttgagaa gttagcagct
      361 gaaagtattg aaaatcctca agtaagaagt gaatttattg atgatgtatt agcagagtct
      421 aaaaaaacaa tattaggtca tgaagaagaa attaaggcat tatttgcgac actttctaat
      481 caagaacttg tagataaaat aatgtcaggg gtacgtaagg aagaaattaa tccgaaatgt
      541 acacatctag tagagtatat ggatgataag tatccattct atttagatcc aatgccaaac
      601 ctttatttta ctagagatcc acaagcctca ataggacacg gtataacaat caatcggatg
      661 ttctggagag cacgacgacg agaatcaata tttattcaat atattgtaaa gcatcatcct
      721 agatttaaag atgcgaatat tccaatctgg ttagatcgag attgcccatt caatattgaa
      781 ggcggcgatg aacttgtttt atctaaagat gtcttggcta taggcgtttc agaacgtaca
      841 tctgcacaag ctattgaaaa gttagcgcga cgtatttttg aaaatccgca ggcgacgttt
      901 aaaaaagtag tagcaattga aattccaact agtcgaactt ttatgcactt agatacagta
      961 tttacaatga tagattatga caaatttaca atgcattcag ccattttaaa ggcagaaggc
     1021 aatatgaata tatttattat tgaatatgat gacgtaaata aagatattgc catcaaacaa
     1081 tctagtcatt taaaagatac tttagaagac gtactaggta tagatgatat ccaattcatt
     1141 ccaacaggaa atggtgatgt cattgatggt gctagagagc aatggaatga tggctcaaat
     1201 acattatgta taagaccagg cgttgtagtg acttacgata gaaactatgt atcgaatgat
     1261 ttattgagac aaaaaggcat taaagtcatt gaaatatctg gtagcgagtt ggtacgtgga
     1321 cgtgggggcc ctagatgtat gagtcaacca ttattcagag aagacattta acgtaataaa
     1381 ttatagaaac gagggtgaaa ataatgacag aaattcaaaa accgtatgat ttaaaaggca
     1441 gatcattatt aaaagaaagt gattttacca aagcagaatt cgaaggactt attgattttg
     1501 caattacatt aaaagagtat aagaaaaacg gtattaagca tcactactta tctggaaaaa
     1561 atattgcact actattcgaa aagaattcga cgagaacgcg tgctgcgttt acagttgcgt
     1621 ctattgattt aggtgcgcat ccagaatttt taggaaaaaa tgatattcaa ttaggcaaaa
     1681 aagaatctgt agaggatact gcgaaagtat taggtagaat gttcgatggt attgaattcc
     1741 gtggtttttc acaacaagct gttgaagatt tagcgaagtt ctctggtgta ccggtgtgga
     1801 atggattaac agacgattgg catcctacac aaatgttagc tgattttatg acaataaaag
     1861 agaattttgg atatctagaa ggaataaact taacttacgt tggagatgga cgtaataata
     1921 ttgcgcattc attaatggta gcaggtgcta tgttaggtgt taatgtaaga atttgtacac
     1981 ctaaatcatt aaatccaaaa gaggcatatg ttgatattgc aaaagaaaaa gcgagtcaat
     2041 atggtggttc agtcatgatt acggataata ttgcagaagc agttgaaaat acagatgcta
     2101 tatatacaga tgtttgggta tcgatgggtg aagaaagtga atttgaacaa cgtattaatt
     2161 tattaaaaga ctatcaagtg aatcaacaga tgtttgattt aacaggtaaa gattcaacga
     2221 tattcttaca ttgtttacca gcattccatg atacaaatac actttatgga caagaaattt
     2281 atgaaaaata tggattagct gaaatggaag ttacagacca aatctttaga agtgaacatt
     2341 caaaagtgtt tgatcaagct gaaaatagaa tgcatacaat taaggcagta atggcagcaa
     2401 cattggggag ttaatcacta aatggaacga tatgaatatg atgtgtctga tgatataagt
     2461 gtcatgtaca gacacctcat attggtatta aaggagaaat gaatatgaac gaatcaggag
     2521 ataacaaact cagtaaatct tctttaattg gactagttat aggatccatg attggtggcg
     2581 gtgcgttcaa tataatgtct gatatgggcg gtaaagccgg tggattagcc attattattg
     2641 gttggattat tacagctata ggaatgattt cattagcgtt cgtatttcaa aatttaacca
     2701 atgaacggcc ggagctagac ggtggtattt atagttatgc tcaagcagga tttggcgatt
     2761 ttgtaggatt tatcagtgct tggggatatt ggttctcagc gtttttaggc aatgttgcct
     2821 atgcaacact attgatgtca gcagtaggta actttttccc gatttttaaa ggaggcaaca
     2881 cattaccaag tgttattgtc gcctcgttac tactctgggg tgtccatttc ttgattttaa
     2941 aaggcgttga aacagcagca tttatcaata gtattgttac tgttgcaaag ttaataccga
     3001 ttttacttgt aatcatatgc atgataattg cattcaattt tgacactttt aaaacaggct
     3061 ttttcagtat gacgtcagag ggtgtattgc catttagttg ggcgagcaca atgagccaag
     3121 ttaaaagtac gatgctagtg acagtttggg tgtttatcgg tatcgaaggt gcagtaattt
     3181 tttctagtag agctaaaaat aaaaaagatg taggtagtgc cacggttata ggacttatat
     3241 cagttttaat tatctatttc ttattaactg tattagctca aggcgtgatt ttgcaaaatc
     3301 atatttcgca attagattcg ccaagtatgg cacaggtgct tgcaactatt gtaggtggtt
     3361 ggggatctac acttgtaaat attggtttaa ttatttcggt actaggtgca tggttaggat
     3421 ggacactgct tgctggtgaa ttacctttca ttgttgcaaa agatggatta tttccaaaat
     3481 ggtttgctaa agaaaataaa aatggagcac ctgtaaatgc actgcttatt accaatatat
     3541 tagtacaatt atttttaata agtatgctat ttacacagag tgcgtatcaa tttgcatttt
     3601 cactagcatc aagtgctatt ttataccctt acatgttcag tgcattttac caagttaaat
     3661 acactttaga gcatcgacag caagcaacta ctaaacaatg gacgattggt atcatagcct
     3721 caatttatgc tatatggctt atatatgcag caggtatcaa ttacttatta ttgacgatgt
     3781 tactttatat tccagctctt cttgtttata caatcgttca aaagaataat cagacacgtt
     3841 tgattaaatc agactatatt ctttttatga ttattatcgt acttgcagtt atcgggttaa
     3901 ttaagttatt gatgggaacg ataaatgttt tttaaaagga gcgacaaaaa tatgaaagag
     3961 aaaattgtca ttgcattagg cggtaatgcg atacagacaa cagaagcaac agctgaagca
     4021 caacaaacag ctattagatg tgcgatgcaa aaccttaaac ctttatttga ttcaccagcg
     4081 cgtattgtca tttcacatgg taatggtcca caaattggaa gtttattaat ccaacaagct
     4141 aaatcgaaca gtgacacaac gccggcaatg ccattggata cttgtggtgc aatgtcacag
     4201 ggtatgatag gctattggtt ggaaactgaa atcaatcgca ttttaactga aatgaatagt
     4261 gatagaactg taggcacaat cgttacacgt gtggaagtag ataaagatga tccacgattt
     4321 gataacccaa ctaaaccaat tggtcctttt tatacgaaag aagaagttga agaattacaa
     4381 aaagaacagc cagactcagt ctttaaagaa gatgcaggac gtggttatag aaaagtagtt
     4441 gcgtcaccac tacctcaatc tatactagaa caccagttaa ttcgaacttt agcagacggt
     4501 aaaaatattg tcattgcatg cggtggtggc ggtattccag ttataaaaaa agaaaatacc
     4561 tatgaaggtg ttgaagcggt tatagataaa gattttgcta gtgagaaatt agcaacgctg
     4621 attgaagcag ataccttaat gattcttacg aatgtagaaa atgtatttat taactttaat
     4681 gaacctaatc aacaacaaat cgatgatatt gatgtagcaa cactgaaaaa atacgcggca
     4741 caaggtaagt ttgtggaagg atcgatgttg ccaaaaatag aagctgcgat acgatttgtt
     4801 gaaagtgggg aaaacaaaaa agttatcatt accaatttag agcaggcata cgaagctttg
     4861 attggtaata aaggtacaca cattcacatg tagcacttta tcacgcgaca aaacattaaa
     4921 tatgtttctc cgttgattca aatgaaaaag ttgtctgctg acactttgca aggtttgaag
     4981 gagtttaact tatgacagaa aactttattt tgggtagaaa taataaatta gaacatgaac
     5041 taaaggcatt agcagattac attaatatac catatagtat attacaacca tatcaaagtg
     5101 aatgttttgt cagacattat acgaaaggcc aagttattta tttttcgcca caagaaagta
     5161 gcaatattta ctttttaatt gaaggtaaca ttattagaga acattacaat caaaatggag
     5221 atgtatatcg ttattttaat aaagagcaag tattatttcc aatcagtaac ttatttcatc
     5281 cgaaagaggt taacgaattg tgtacagcat taaccgattg tacagttctt ggattgccta
     5341 gagaattgat ggcctttttg tgcaaagcta atgatgatat atttttgaca ctttttgcat
     5401 taataaatga taatgagcag caacacatga actataacat ggcattaaca agtaaatttg
     5461 ctaaagatcg aattatcaaa ttgatatgcc atctatgtca gacagtagga tacgatcaag
     5521 atgaatttta tgaaatcaaa cagtttttaa ctattcaact catgagtgat atggctggga
     5581 tttcccggga aacagctggt catattattc atgaacttaa agatgaaaaa cttgttgtta
     5641 aagatcataa aaattggtta gtaagcaaac atttattcaa tgatgtatgt gtttaatata
     5701 caatgtaaaa tgaataagtt gaacatgagg tctaacgtac atttatacgt taggcctttt
     5761 ttgctagcat gatgaataat ttaaaatgtt agttaaattt gattgttgaa attacagtaa
     5821 aatttaaggt gatgaaaaat ttagaacttc taagtttttg aaaagtaaaa aatttgtaat
     5881 agtgtaaaaa tagtatattg atttttgcta gttaacagaa aattttaagt tatataaata
     5941 ggaagaaaac aaattttacg taattttttt cgaaaagcaa ttgatataat tcttatttca
     6001 ttatacaatt tagactaatc tagaaattga aatggagtaa tatttttgaa aaaaagaatt
     6061 gattatttgt cgaataagca gaataagtat tcgattagac gttttacagt aggtaccaca
     6121 tcagtaatag taggggcaac tatactattt gggataggca atcatcaagc acaagcttca
     6181 gaacaatcga acgatacaac gcaatcttcg aaaaataatg caagtgcaga ttccgaaaaa
     6241 aacaatatga tagaaacacc tcaattaaat acaacggcta atgatacatc tgatattagt
     6301 gcaaacacaa acagtgcgaa tgtagatagc acaacaaaac caatgtctac acaaacgagc
     6361 aataccacta caacagagcc agcttcaaca aatgaaacac ctcaaccgac ggcaattaaa
     6421 aatcaagcaa ctgctgcaaa aatgcaagat caaactgttc ctcaagaagc aaattctcaa
     6481 gtagataata aaacaacgaa tgatgctaat agcatagcaa caaacagtga gcttaaaaat
     6541 tctcaaacat tagatttacc acaatcatca ccacaaacga tttccaatgc gcaaggaact
     6601 agtaaaccaa gtgttagaac gagagctgta cgtagtttag ctgttgctga accggtagta
     6661 aatgctgctg atgctaaagg tacaaatgta aatgataaag ttacggcaag taatttcaag
     6721 ttagaaaaga ctacatttga ccctaatcaa agtggtaaca catttatggc ggcaaatttt
     6781 acagtgacag ataaagtgaa atcaggggat tattttacag cgaagttacc agatagttta
     6841 actggtaatg gagacgtgga ttattctaat tcaaataata cgatgccaat tgcagacatt
     6901 aaaagtacga atggcgatgt tgtagctaaa gcaacatatg atatcttgac taagacgtat
     6961 acatttgtct ttacagatta tgtaaataat aaagaaaata ttaacggaca attttcatta
     7021 cctttattta cagaccgagc aaaggcacct aaatcaggaa catatgatgc gaatattaat
     7081 attgcggatg aaatgtttaa taataaaatt acttataact atagttcgcc aattgcagga
     7141 attgataaac caaatggcgc gaacatttct tctcaaatta ttggtgtaga tacagcttca
     7201 ggtcaaaaca catacaagca aacagtattt gttaacccta agcaacgagt tttaggtaat
     7261 acgtgggtgt atattaaagg ctaccaagat aaaatcgaag aaagtagcgg taaagtaagt
     7321 gctacagata caaaactgag aatttttgaa gtgaatgata tatctaaatt atcagatagc
     7381 tactatgcag atccaaatga ctctaacctt aaagaagtaa cagaccaatt taaaaataga
     7441 atctattatg agcatccaaa tgtagctagt attaaatttg gtgatattac taaaacatat
     7501 gtagtattag tagaagggca ttacgacaat acaggtaaga acttaaaaac tcaggttatt
     7561 caagaaaatg ttgatcctgt aacaaataga gactacagta ttttcggttg gaataatgag
     7621 aatgttgtac gttatggtgg tggaagtgct gatggtgatt cagcagtaaa tccgaaagac
     7681 ccaactccag ggccgccggt tgacccagaa ccaagtccag acccagaacc ggaaccaagc
     7741 ccagacccgg atccggattc ggattcagac agtgactcag gctcagacag cgactcaggt
     7801 tcagatagcg actcagaatc agatagcgat tcggattcag acagtgattc agattcagac
     7861 agcgactcag aatcagatag cgattcagaa tcagatagcg actcagattc agatagcgat
     7921 tcagattcag atagcgattc agattcagat agcgattcgg attcagacag tgattcagat
     7981 tcagacagcg actcagaatc agatagcgac tcagaatcag atagtgagtc agattcagac
     8041 agtgactcgg actcagacag tgattcagac tcagatagcg attcagactc agatagcgat
     8101 tcagactcag acagcgattc agattcagac agcgactcag attcagacag cgactcagac
     8161 tcagacagcg actcagattc agatagcgat tcagactcag acagcgactc agactcagac
     8221 agcgactcag actcagatag cgactcagat tcagatagcg attcagactc agacagcgac
     8281 tcagattcag atagcgattc ggactcagac agcgattcag attcagacag cgactcagac
     8341 tcggatagcg attcagattc agatagcgat tcggattcag acagtgattc agattcagac
     8401 agcgactcag actcggatag cgactcagac tcagacagcg attcagactc agatagcgac
     8461 tcagactcgg atagcgactc ggattcagat agcgactcag actcagatag tgactccgat
     8521 tcaagagtta caccaccaaa taatgaacag aaagcaccat caaatcctaa aggtgaagta
     8581 aaccattcta ataaggtatc aaaacaacac aaaactgatg ctttaccaga aacaggagat
     8641 aagagcgaaa acacaaatgc aactttattt ggtgcaatga tggcattatt aggatcatta
     8701 ctattgttta gaaaacgcaa gcaagatcat aaagaaaaag cgtaaatact tttttaggcc
     8761 gaatacattt gtattcggtt tttttgttga aaatgatttt aaagtgaatt gattaagcgt
     8821 aaaatgttga taaagtagaa ttagaaaggg gtcatgacgt atggcttata tttcattaaa
     8881 ctatcattca ccaacaattg gtatgcatca aaatttgaca gtcattttac cggaagatca
     8941 aagcttcttt aatagcgata caactgttaa accattaaaa actttaatgt tgttacatgg
     9001 attatcaagt gatgaaacga catatatgag atatacaagc atagaaaggt atgcgaatga
     9061 acacaaatta gctgtgatta tgcccaatgt ggatcatagc gcatatgcta acatggcata
     9121 tggtcatagc tattatgatt atattttgga agtgtatgat tatgttcatc aaatatttcc
     9181 actttccaaa aagcgtgatg acaattttat agcaggtcac tctatgggag gatatggcac
     9241 aattaaattt gcattaacac aaggggataa atttgccaaa gctgtaccat tatctgctgt
     9301 gtttgaagcg caaaatttaa tggatctaga gtggaatgat ttttcaaaag aggccataat
     9361 tggcaatctt tcaagtgtta aaggaactga acatgatccg tattacttgc tagacaaagc
     9421 tgtagctgaa gataaacaaa ttccaaaatt gctcattatg tgtggtaaac aagacttttt
     9481 atatcaagac aacttagatt ttatcgatta tttatcacgc ataaatgttc cttatcaatt
     9541 tgaagatgga ccaggagatc atgattatgc atattgggat caagcgatta agcgtgctat
     9601 aacatggatg gtgaatgatt aattatttct tggaaaatat gtggctgcat taaatacaca
     9661 gagtgagaga tacaaactat ttacgcacga ctaacatttc taagtgttta aattattttt
     9721 gtattaatat gattggcgca atttgctgat acacaaaaat gtttctcgtg aaacttagat
     9781 ttagcttata gttttatcat catttgtatg acttacatta taaattttat tataatgagg
     9841 ttaacgcttt gaaaggagtc atcatcatgt cgaccaataa aaacgattat gagcatatgt
     9901 tgttttattt tgcatataaa acctttatta ctaccgctga tgaaattata gagaagtatg
     9961 gtatgagtcg tcagcatcat cgttttttgt tttttatcaa taaattacct ggtattacta
    10021 ttaaatcatt actagaaata ttagaaattt ctaaacaagg atcacatgca acacttcaaa
    10081 aattaaaaga gcaaggtctc attattgaaa aagttttaga gactgatcga cgtgtcaaaa
    10141 aattatattc gacggataaa ggcgatcaac tcattgctga attgaacaag gcgcaagatg
    10201 aattattgca aaatatatat caacaagtcg gttcggattg gtatgatgtg atggaagcat
    10261 tggctaaagg gcgacctggc tttgatttta ttaagcattt gaaagatgaa aaagaaagct
    10321 agcatcagaa atgttaaaaa tcttcgcatt cttaaattta aaaaatatgt caaaaagtgt
    10381 ataataaaaa catataattt aattgaactc agtttcaaca catcttagaa aggagtttga
    10441 atgatgaaaa aattagcagt tattttaaca ttagttggcg gtttatactt cgcatttaaa
    10501 aaataccaag aacgtgttaa ccaagcacct aacattgagt actaaattaa accataaaaa
    10561 attcccgaac accttgttat agtgctcggg aattttttta tgctttactt gaatatatca
    10621 aatattattt ttgcgctttc tgtattttcg atattaccac taaatgattc tgatctaggt
    10681 ccgtaagcgt aggtattaac atcctcgcct gtatgtccat cggaagtcca ccctgtataa
    10741 gatttatcat ttactggctt ctgaatagcg tgttgtaggg cttttgtttg cgtttctact
    10801 tctgcggatt tttcgtcttt ttctttttta agtagtcttt ttagcttttt attctctttt
    10861 ttaacctttt tcatatcatc ttgtgaaaat tcaaatccat aaccttcatt aataactttt
    10921 tcagggtctt cacctttagc cattttttct gtcatatatg atccagagtg tttcatagat
    10981 ttaatcggtt gaggattcca ttcgtatcct ttatctttac caattgttag accaccagta
    11041 gagtgatctg ctgttgcaac gacaagtgta tctttatgtt tcttagcata ttgaatggcg
    11101 tcatcgaatg ctttttcaaa accttccatt tcagacataa cgcctgtaat atcgttggaa
    11161 tgcgctgatt tatctataga agcaccttcg accattaaaa agaatccttt tttattgcgc
    11221 tcaagcttac taagtgcact ttgttgcata tcagctaatg atggttcgtc tttagaagca
    11281 tctattgcaa gtggcatatt tttatctgca aacaaaccaa gaactttatc tttatcagat
    11341 tttgataact ccttactgtt cgtggcaagg tcgtaaccat cttttttgaa ttttttatct
    11401 aaattgccat tacttttacc gaaatattta gcgccgccgc ctaataaaac atcaacttta
    11461 tgctttccgt tgattttatc tttataaaat tgtttagcga tttcgttttt atcatctcta
    11521 gaagtcacgt gtgcagcata tgctgctggt gttgcatctg ttaattcagc tgttgaaaca
    11581 agaccagtcg acttaccttt ttcttttgca cgttcaagca ccgtctttac tttctgcttg
    11641 ttactgtcaa caccgatggc accattatat gtcttatgac cagaactaaa ggctgttccg
    11701 ccagctgcag aatcagtaat attctgtttt gggtcatttg aatatgtacg atttgtgcct
    11761 tttaaatatg aatcaaaagc agtaggggtc atttctttag catgcggatc atttttataa
    11821 taacgataag ctgtgttaaa tgatggaccc atgccatcgc caactaaaaa gataacattt
    11881 tttggatttt tagtattacc aaccgcgaaa ctttcatctt tagaactttt atcggattgc
    11941 gcaattgcag gtgtgacaga actaaaaacc gttgacacga taataaggtt agcaactgca
    12001 aattttgtgg cttttttaac tgataacata agacatcctc ctgagtatat gactatgtct
    12061 tcagtgtaaa agaggaattt tgagcaatta tgtagtttta gttaaaaata tgtaaacaga
    12121 gtgatttaga ataacaaaaa atgaatatat atgacaattt gttatagaaa gcgttagaat
    12181 agaagcgtgt gaaaatatag aattaaatat aatttgaggt ggaaaaatga tactagtaat
    12241 gttatctcca ttattaatca tattctttat agtgttgtct attttagaag agcgtaaacg
    12301 tacgaagaaa aagcaactcg agaaagaaaa agcaaataca ctaaatcaaa atacaaatga
    12361 cacggaaagt tcaaatcaag agccgtcatt gcagcaggat aaagaacaaa aagataacaa
    12421 aggataattc aattgaagga agaagattat agatgaaaat attaattgtt gaagatgatt
    12481 ttgttatagc agagagttta gcatctgaac ttaaaaaatg gaattacggt gttattgtcg
    12541 ttgaacaatt tgatgatata ctgtctatct ttaaccaaaa tcaacctcag cttgtattgc
    12601 tagatattaa tttgccaacg ttaaatggtt ttcattggtg tcaagaaatc cgaaaaacat
    12661 ctaatgtgcc aattatattt attagttccc gtattgataa tatggaccaa attatggcaa
    12721 tacaaatggg gggagatgat tttatcgaaa agccatttaa cttgtcatta acgattgcca
    12781 aaattcaagc attattgaga cgaacttatg acttgtcagt agctaatgat tcattgacgg
    12841 ttaaaggttg tacattaata ttagatgaag caaaagtcgt gtatcaagaa caaaacatac
    12901 agctatcttt gactgaatta caaatattaa agttattatt tcaaaatgaa gataaatatg
    12961 taagtagaac tgctttaatt gaaaaatgtt gggaatcaga aaacttcata gatgataaca
    13021 cattagctgt taacatgacg cgcctgcgta aaaaattaaa tactattggc gttaatgatt
    13081 ttatcattac aaagaaaaat gtcggatata aagtataggg tgaatgcaat gacctttctt
    13141 aaaagtatta ctcaggaaat agcaatagtc atagttattt ttgctttgtt tggcttaatg
    13201 ttttacctgt atcatttgcc attagaagca tatttactag cacttggcgt tattttatta
    13261 ttattactca tattcatagg tattaaatat ttaagttttg taaaaactat aagccaacaa
    13321 caacaaattg aaaacttaga aaatgcgttg tatcagctta aaaatgaaca aattgaatat
    13381 aaaaatgatg tagagagcta ctttttaaca tgggtacatc aaatgaaaac acccattact
    13441 gcagcacaac tgttacttga aagagatgag cctaatgttg ttaatcgtgt tcgtcaagag
    13501 gttattcaaa ttgataacta tacaagttta gcacttagtt atttaaagtt attaaatgaa
    13561 acttctgata tttctgtcac taaaatttcg attaataata tcattcgccc aattattatg
    13621 aaatattcaa tacagtttat tgatcaaaaa acaaaaatcc attatgaacc ttgtcatcac
    13681 gaagtattaa ctgacgttag atggacctct ttaatgatag aacaattaat aaataatgca
    13741 cttaagtatg cgagaggtaa agatatatgg attgaatttg atgagcaatc caatcaatta
    13801 cacgtaaaag ataatggtat cggtattagt gaagcggact tgcctaaaat atttgataag
    13861 ggctattcag gttataatgg ccagcgccaa agtaactcaa gtgggattgg tttatttatc
    13921 gtaaaacaaa tttcaacaca cacaaaccat cctgtttcag tcgtatctaa acaaaatgag
    13981 ggtacaacat ttacgattca atttccagat gaataaaaac tttcaatatt gtaagtatac
    14041 tagtaacatt tttttactaa tttaaatgtt attagtattt ttttgtttta atatagaact
    14101 aacaaagaaa tgaggtgcat gccatgttgc tagaagtgaa acatgtaaaa aaggtttatg
    14161 gtaaaggttt gaatgctacg acagcactta atcaaatgaa tttatcagtt ggagctggtg
    14221 aatttgttgc aattatgggt gagtctgggt cagggaagtc tacactacta aatttaattg
    14281 cttcttttga tggactaact gaaggtgaca ttattgtgga tggcgcacat ttaaataata
    14341 tgaaaaataa aagtaaagca ttgtatcgtc aacaaatggt aggttttgtt tttcaagatt
    14401 ttaatctttt accaacaatg acgaataaag aaaatataat gatgccatta attttagctg
    14461 gtgctaaacg aaaagatata gaacaaaggg tacatcagtt ggcagtacaa ttacatttag
    14521 agggattctt aaacaagtat ccttctgaaa tctctggggg tcagaagcaa cgcattgcca
    14581 ttgcacgtgc attagttact aagccgacga ttttactagc cgatgaacct acaggtgcac
    14641 ttgattcaaa aacatcaaag gcattgatga tgttatttca agagattcat caattggaac
    14701 agacaatttt aatggtaact cattcaaata tcgatgcgtc ttatgcagag cgagtcattt
    14761 ttattaaaga tgggcgtcta tatcatgaaa tatatcgtgg tgaagaaagt caattagctt
    14821 ttcaacaacg aataacagat agcttagcac ttgtgaatgg aggaagtgtc aatatatgaa
    14881 gttaagattg ttatggcaca tagtgcgacg tcaatttatt acgcagcgac ttgtaatcat
    14941 tccattcatt ttagcggtaa gtgtactatt catgattgaa tatacgcttg tgtcaattgg
    15001 gttaaatagc tacataaaac agaagaatga cttcctagta ccatttatta tcatagctaa
    15061 tttttttatg gcgcttttaa cttttatttt tattttctat gcaaatcact ttatgatgtc
    15121 acaaagacga aaagagttta gcatttttat gacattgggc atgaccaaga aaagtatgcg
    15181 tttaattgta gtgatggaaa ctatcttaca atttgtgata atttcagtcg ttagtattgc
    15241 cggcggatac ttacttggtg cgatattttt cttgtttata cagaaaataa tgggcagtga
    15301 agttgcgacg ttaaggtatt atccatttga ctctgtagcg atgtttatta ctttgattat
    15361 cattgctgta ttaatgggca tgctacttat attcaacttg tttagtatta attttcaacg
    15421 gccgataact tatcaacatc gttccgattc tagtgtcata tcacgatggt tgcgttacgt
    15481 tttaattgtt ataggaagcg cagcactata tttaggttac tttattgcat tacaacaaga
    15541 tacgacgttt ggtgcctttt ttaaaatatg gattgtcata ggattagtta ttatcggtac
    15601 ttatgcattt tttgtaggta taagtgaaat aattattagt atattgcagc aggtatcaaa
    15661 agtttactat catccacggt atttttttgt ggtagttggg atgcgtgtac gtcttaaaat
    15721 gaatgcagtc agtcttgcaa caatcacttt gctgtgtaca tttttgattg taacgctcac
    15781 aatgacatta acaacctatc gtgatatgaa tcataccatt acgaaattga ttacgaatga
    15841 ttatgatttg tcatttagcg acaattctaa gtcacaaata gaacgtcaac aaacaattga
    15901 gaatattaaa cgtgatatac agggttcggt taatgcaaaa gacttcaaag tatatgaaac
    15961 gacattattt agagcttctt tagaaaagaa tcgtgcttat tacaagttaa aacaggctag
    16021 tgatgatatt ccaatagact tcattggtaa tgaaggcgcg atattttcaa gacaatcagt
    16081 aatgattaca gcttttactg cacaagatta taatcgatat caccaaaaca aattacactt
    16141 agacaatcaa agtataggta tgattacgaa tgtacctatt tttaaaaagc agtctaaggt
    16201 cggtcttaat aatgaagtct ttaaagtgaa acaacttgac gcacatgcgt taaatttgat
    16261 gatgattcaa gatagtatgg ttttaattgt taaagataac aatcaattac aaaaagtaaa
    16321 aggattttat gcacatgaac aaaagaattc gacaatatcg ataaacttta atgtatttgg
    16381 aaagacgaaa ttaacaacat cacaaataca aaagctatcg aaaaaatacg atgcgtcaat
    16441 aaattctaaa tcagaaatgc taatggtttg ggatcgtttg acaggtggac taatatttgt
    16501 aggtggcgta gtatcaattg tacttgtcat tggaatattt ctaatgatgt attataaaca
    16561 agtctcagaa gcctatgcaa atcagcacaa ttatgacatt atgaaaaaac ttggtttaga
    16621 taatggaaga atagcaaaga taacacgaaa tcaaatgact ttcttgttcg caataccaat
    16681 aacagtagca ctgattcaca cattaatttc tagtaacatt gtatatacgt tactaaatat
    16741 gataggtatt aataaccatc atatattttt aacaagttac gtactagctg tcattataat
    16801 ttcgtttcta tatatggcga tgtacaaaat aacttcctat atttatgcta aagttattca
    16861 tcaacaacgc aattaaccat gtttcagtga aatcacaatc aatcatgtat tttcactgaa
    16921 atatattata ttaaaaagaa acctaaaaac attacaaata acaataacaa atttcaaccg
    16981 ataagataaa atgtcttgtc ggtttttaat ataacaagtg atattttgga aatgccaaaa
    17041 atatatagat tgtttatata aaatgattta aaatgtttta taacaacata ttttatgcaa
    17101 cacataaaat gggtaactaa catcacgaaa tcattgtatt tgttgttaca ctgatgaagc
    17161 gatgtcacaa agaaatgtcg ttatcaaacg aagcccgaaa tacatactga tactcgaaat
    17221 aatgta
//
LOCUS       DANJFZ010000054        16497 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.054, whole genome shotgun sequence.
ACCESSION   DANJFZ010000054 DANJFZ010000000
VERSION     DANJFZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.054"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            198..6758
                     /gene="sasC"
                     /locus_tag="PWN45_001547"
     CDS             198..6758
                     /gene="sasC"
                     /locus_tag="PWN45_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDL0623316.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6875..8143)
                     /locus_tag="PWN45_001548"
     CDS             complement(6875..8143)
                     /locus_tag="PWN45_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0623317.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8550..10211
                     /locus_tag="PWN45_001549"
     CDS             8550..10211
                     /locus_tag="PWN45_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0623318.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10208..10903
                     /locus_tag="PWN45_001550"
     CDS             10208..10903
                     /locus_tag="PWN45_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0623319.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10920..11342
                     /locus_tag="PWN45_001551"
     CDS             10920..11342
                     /locus_tag="PWN45_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0623320.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            12092..13501
                     /gene="sapep"
                     /locus_tag="PWN45_001552"
     CDS             12092..13501
                     /gene="sapep"
                     /locus_tag="PWN45_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDL0623321.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            13505..14353
                     /gene="dat"
                     /locus_tag="PWN45_001553"
     CDS             13505..14353
                     /gene="dat"
                     /locus_tag="PWN45_001553"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDL0623322.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            14903..15694
                     /locus_tag="PWN45_001554"
     CDS             14903..15694
                     /locus_tag="PWN45_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL0623323.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            15709..16353
                     /gene="trmB"
                     /locus_tag="PWN45_001555"
     CDS             15709..16353
                     /gene="trmB"
                     /locus_tag="PWN45_001555"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL0623324.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELYEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
BASE COUNT         6176 a         2505 c         3002 g         4814 t
ORIGIN      
        1 ttaagatttt aattacaaaa cggaaactaa attgtaataa aataaaactt tattttataa
       61 aatgatgatg ataaaattga gtgaacttaa aatattgtac aaaataatat agctataaat
      121 ataatatgag ggagcgtata tttttagcat aattcttaac aacacagcag agaacagaca
      181 accaggagga aaatgaaatg aatttgttaa agaaaaataa atatagtatt aggaagtata
      241 aagtaggcat attctctact ttaatcggaa cagttttatt actttcaaac ccaaatggtg
      301 cacaagcctt aactacggat aataatgtac aaagcgatac taatcaagca acacctgtaa
      361 attcacaaga taaagatgtt gctaataata gaggtttagc aaatagtgcg cagaatacac
      421 ctaatcaatc tgcaacaacc aatcaagcaa cgaatcaagc attggttaat cataataatg
      481 gtagtatagt aaatcaagct acgccaacat cagtgcaatc aagtacgcct tcagcacaaa
      541 acaataatca tacagatggc aatacaacag caactgagac agtgtcaaac gctaataata
      601 atgatgtagt gtcgaataat accgcattaa atgtaccaac taaaacaaat gaaaatggtt
      661 caggaggaca tctaacttta aaggaaattc aagaagatgt tcgtcattct tcaaataaac
      721 cagagctagt tgcaattgct gaaccagcat ctaatagacc gaaaaagaga agtagacgtg
      781 cggcaccggc agatcctaat gcaactccag cagatccagc ggctgcagcg gtaggaaacg
      841 gtggtgcacc agttgcaatt acagcgccat atacgccaac aactgatcct aatgccaata
      901 atgcaggaca aaatgcacct aacgaagtgc tgtcatttga tgacaatggt attagaccaa
      961 gtaccaaccg ttctgtgcca acagtaaacg ttgttaataa cttgccgggc ttcacactaa
     1021 tcaatggtgg caaagtaggg gtgtttagtc atgcaatggt aagaacgagc atgtttgatt
     1081 caggagataa taagaactat caagcacaag gaaatgtaat tgcattaggt cgtatacatg
     1141 gaactgatac gaatgaccat ggcgatttta atggtatcga gaaagcatta acagtaaatc
     1201 cgaattctga attaatcttt gaatttaata caatgactac taaaaacggt caaggcgcaa
     1261 caaatgttat tatcaaaaat gctgatacta atgatacgat tgctgaaaag actgttgaag
     1321 gcggtccaac tttgcgttta tttaaagtac ctgataatgt gagaaatctc aaaattcaat
     1381 ttgtacctaa aaatgacgca ataacagatg cgcgtggcat ttatcaacta aaagatggtt
     1441 acaaatacta tagctttgtt gactctatcg gacttcattc tgggtcacat gtttttgttg
     1501 aaagacgaac aatggatcca acagcaacaa ataataaaga gtttactgta acaacatcat
     1561 taaagaataa tggtaattct ggtgcttctc tagatacaaa tgactttgta tatcaagttc
     1621 aattacctga aggtgttgaa tatgtgaaca attcattgac taaagatttt ccaagtaaca
     1681 attcaggcgt tgatgttaat gatatgaatg ttacatatga tgcagcaaat cgtgtgataa
     1741 caattaaaag tactggagga ggtacagcaa actctccggc acgacttatg cctgataaaa
     1801 tactcgattt aagatataaa ttacgtgtaa ataatgtgcc gacaccaaga acagtaacat
     1861 ttaacgagac attaacgtat aaaacatata cacaagattt cattaattca gctgcagaaa
     1921 gtcatactgt aagtacaaat ccatatacta tcgatatcat catgaataaa gatgcattac
     1981 aagccgaagt tgacagacgt attcaacaag ctgattatac atttgcgtca ttagatatct
     2041 ttaatggtct gaaacgacgc gcacaaacga ttttagatga aaatcgtaac aatgtaccat
     2101 taaataaaag agtttctcaa gcatatattg attcattaac taatcaaatg caacatacgt
     2161 taattcgaag tgttgatgct gaaaatgcag ttaataaaaa agttgaccaa atggaagatt
     2221 tagttaatca aaatgatgaa ttgacagatg aagaaaaaca agcagcaata caagttatcg
     2281 aggaacataa aaatgaaata attggtaata ttggtgacca aacgactgat gatggcgtta
     2341 ctagaatcaa agatcaaggt atacagacct taagtgggga tactgcaaca ccggttgtta
     2401 aaccaaatgc taaaaaagca atacgtgata aagcaacgaa acaaagggaa attatcaatg
     2461 caacaccaga tgttactgaa gacgagattc aagatgcact aaatcaatta gctacggatg
     2521 aaacagatgc tattgataat gttacgaatg ctactacaaa tgctgacgtt gaaacagcta
     2581 aaaataatgg catcaatact attggagcag ttgttcctca agtaactcat aaaaaagctg
     2641 caagagatgc aattaaccaa gcaacagcaa cgaaaagaca acaaataaat agtaatagag
     2701 aagcaactca ggaagagaaa aatgcagcat tgaacgaatt aactcaagca accaaccatg
     2761 ctttagaaca aatcaatcaa gcaacaacaa atgctgatgt tgataacgcc aaaggagatg
     2821 gtctaaatgc cattaatcca attgctcctg taactgttgt taagcaagct gcaagggatg
     2881 ccgtatcaca tgatgcacaa caacatatcg cagagatcaa tgctaatcct gatgcgactc
     2941 aagaagaaag acaagcagca attgacaaag tgaatgctgc tgtaactgca gcaaacacaa
     3001 acattttaaa cgctaatacc aatgctgatg ttgaacaagt aaagacaaat gcgattcaag
     3061 gaatacaagc aattacacca gctacaaaag taaaaacaga tgcaaaaaat gccatcgata
     3121 aaagtgcgga aacgcaacat aatacgatat ttaataataa tgatgcgacg ctcgaagaac
     3181 aacaagcagc acaacaatta cttgatcaag ctgtagccac agcgaagcaa aatattaatg
     3241 cagcagatac gaatcaagaa gttgcacaag caaaagatca gggcacacaa aatatagtag
     3301 tgattcaacc ggcaacacaa gttaaaacgg atactcgcaa tgttgtaaat gataaagcgc
     3361 gagaggcgat aacaaatatc aatgctacaa ctggcgcgac tcgagaagag aaacaagaag
     3421 cgataaatcg tgtcaataca cttaaaaata gagcattaac tgatattggt gtgacgtcta
     3481 ctactgcgat ggtcaatagt attagagacg atgcagtcaa tcaaatcggc gcagttcaac
     3541 cgcatgtaac gaagaaacaa actgctacag gtgtattaaa tgatttagca actgctaaaa
     3601 agcaagaaat taatcaaaac acaaatgcaa caactgaaga aaagcaagtg gctttaaatc
     3661 aagtggatca agagttagca acggcaatta ataatataaa tcaagctgat acaaatgcgg
     3721 aagtagatca agcgcaacaa ttaggtacaa aagcaattaa tgcgattcag ccaaatattg
     3781 ttaaaaaacc tgcagcatta gcacaaatca atcagcatta taatgctaaa ttagctgaaa
     3841 tcaatgctac accagatgca acgaatgatg agaaaaatgc tgcgatcaat actttaaatc
     3901 aagacagaca acaagctatt gaaagtatta aacaagctaa cacaaatgca gaagtagacc
     3961 aagctgcgac agtagcagag aataatatcg atgctgttca agttgatgta gtaaaaaaac
     4021 aagcagcgcg agataaaatc actgctgaag tggcgaagcg tattgaagcg gttaaacaaa
     4081 cacctaatgc aactgacgaa gaaaagcagg ctgctgttaa tcaaatcaat caacttaaag
     4141 atcaagcaat taatcaaatt aatcaaaacc aaacaaatga tcaggtagac acaactacaa
     4201 atcaagcggt aaatgctata gataatgttg aagctgaagt agtaattaaa ccaaaggcaa
     4261 ttgcagatat tgaaaaagct gttaaagaaa agcaacagca aattgataat agtcttgatt
     4321 caacagataa tgagaaagaa gttgcttcac aagcattagc taaagaaaaa gaaaaagcac
     4381 ttgcagctat tgaccaagct caaacgaata gtcaggtgaa tcaagcagca acaaatggtg
     4441 tatcagcgat taaaattatt caacctgaaa caaaagttaa accagctgca cgtgaaaaaa
     4501 tcaatcaaaa agcgaatgaa ttacgtgcta agattaatca ggataaagaa gcaacagcag
     4561 aagaaagaca agtagcacta gataaaatca atgaatttgt aaatcaagcc atgacagata
     4621 ttacgaataa tagaacaaat caacaagttg atgatacaac aagtcaagcg cttgatagca
     4681 ttgctttagt gacgcctgac catattgtta gagcagctgc tagagatgca gttaagcaac
     4741 aatatgaagc taaaaagcgc gaaattgagc aagcggaaca tgcgactgat gaagaaaaac
     4801 aagttgcttt aaatcaatta gcgaataatg aaaaacgtgc attacaaaac atcgatcaag
     4861 caatagcgaa taatgatgtg aaacgtgttg aaacaaatgg cattgctaca ctaaaaggtg
     4921 tacaacctca tattgtaatt aagcctgaag cacaacaagc aataaaagca agtgcagaaa
     4981 atcaagtaga atcaataaaa gatacaccac atgcaacagt tgatgaatta gatgaagcga
     5041 atcaattaat tagcgacaca ctcaaacaag cgcaacaaga aatagaaaat acaaatcaag
     5101 atgctgctgt tactgatgtt agaaatcaaa caatcaaggc aatagagcaa ataaaaccta
     5161 aagtaagacg taaacgagct gcgcttgata gcattgaaga aaataataaa aatcaactcg
     5221 atgcaatccg aaatacgttg gatactactc aagatgaaag agatgttgct attgatactt
     5281 taaataaaat tgtaaataca attaaaaatg acattgcaca aaacaaaacg aatgcagaag
     5341 tggatcgaac tgagactgat ggcaacgaca acatcaaagt gattttacct aaagttcaag
     5401 ttaaaccagc agcgcgtcaa tctgttggtg taaaagccga agctcaaaat gcactaatcg
     5461 atcaaagcga tttatcaact gaagaagaaa gactagctgc taaacattta gtagaacaag
     5521 cacttaatca ggctattgat cagatcaatc atgcagataa gactgcccaa gttaatcaag
     5581 atagtataaa tgctcaaaat attatttcaa aaattaaacc agcgacaaca gttaaagcaa
     5641 cagcattaca acaaattcaa aatatcgcta caaataaaat taatttaatt aaagcaaata
     5701 acgaagcgac agatgaagaa caaaatattg caatagcaca agttgaaaaa gagttaatta
     5761 aagctaaaca acaaattgct agtgcagtga ctaatgcaga tgtggcatat ttattgcatg
     5821 atgagaaaaa cgaaattcgt gaaatcgaac ctgttattaa cagaaaggcg tctgctcgag
     5881 aacaattgac aacattattc aacgataaaa aacaagcaat tgaagcgaat attcaagcaa
     5941 cggtagaaga aagaaatagt atattagcac agttacaaaa tatttatgac actgctattg
     6001 gacaaattga tcaagatcgt agcaatgcac aagttgataa aacagcatca ttaaatctac
     6061 aaacaataca tgatttagat gtacatccta ttaaaaagcc agatgctgaa aaaacgatta
     6121 atgatgatct tgcacgcgtc actgctttag tgcaaaatta tcgaaaagta agtaatcgta
     6181 ataaggctga tgcattaaaa gctataactg ctttaaaatt acaaatggat gaagaattaa
     6241 aaacagcacg cactaatgct gatgttgatg cagttttaaa acgatttaat gttgcattaa
     6301 gcgatataga agcagtaatt actgaaaaag aaaatagctt actgcgaatt gataacattg
     6361 ctcaacaaac atatgcgaaa ttcaaagcga tcgcaacacc agaacaatta gctaaagtaa
     6421 aagtattaat tgatcaatat gttgcagatg gcaatagaat gattgatgaa gatgcgacat
     6481 taaatgacat caaacaacac acgcaattca ttgttgatga aattttagca attaaattac
     6541 cagctgaagc gacgaaagta tcaccaaaag aaattcagcc agctccaaaa gtttgtacgc
     6601 ctattaaaaa agaagagaca catgaatcgc gcaaagttga aaaagaactt ccaaatacag
     6661 gttctgaagg aatggattta ccattgaaag aatttgcact gattacaggt gcggctttgt
     6721 tagctagaag acgtactaaa aacgaaaaag aatcataatt aacgaaatga ccttaatata
     6781 acgacacttt ttagtgactc agttatatta aggtcatttt tatttgaatg aaaaagttga
     6841 tctactaaag agaatcgaac caaattttat actattattc cattgatgca tgtgagtaat
     6901 gtccggcata taatccagcg acatgtcctg ttacgagtgc acttgtaata ttataaccac
     6961 cagtataacc atgtatatct aatacttcac cacataaaaa taatcccgga actaatttag
     7021 acatcattgt tttaggttga atttctttaa gtgacacacc accacctgtg acaaatgcct
     7081 tatctatagg taatgtcccg ttcaccttaa atacaaaccc tttaaacata tttactaagt
     7141 cgttcaattg ttgatttgat aagtgatgtg atgtggtatt ttcatcgatt cctgcttgtt
     7201 ccagcatgaa cagtaagtag cgctcttcaa ttagaccatg caaactgttt ttaatgattt
     7261 tatctggtgt gtccgataat aatgatgtga tgtgttgttt taattgttca tggtttaatt
     7321 caggaaatgc atcgattgcc atagaaatgt gctgtgtctt ttgatttttt tgttctttat
     7381 aaacaaactg actacatctt aatgcagctg gaccactgat accaaaatga gtaaataaca
     7441 tatccatttg atgactgatg tgttttttac catttttctt aagtactgac aattcaacat
     7501 cttttaaact taaacctttt agacgattgg atttgatgaa aggttcagct gatgtaattg
     7561 gaacttcggt cgggaataac tcagtaatgg tatgacctaa atcttgtgcg aacttataac
     7621 catcaccagt tgaaccagtt tgagggacac ttgtaccacc tgtagcaatc actagcgaat
     7681 ggctttcata actattattt tgagtatgta cagtgaaagt ttggtctgta ttaacttcga
     7741 ttctactaac agcttcttct tctttaatcg ttacatgttg gcgttcgata gttgtcacta
     7801 atgtatcaac cacgtcttgt gctttgttgg aaactggaaa catacgcccg tgatcttctt
     7861 cttttaattt aacaccccta gactcaaaaa aatctatgat ggattcatta tcaaaaattg
     7921 aaaagggact atataaaaat ttcccatttc caggaatgtt cttaataatt tcagcatatg
     7981 gtaatcgatt agttacgtta catctaccgc caccagatat tttgagttta cgacctagac
     8041 ctttcttttt ttcaatgagt aacacactgc tactttgttc gcttgcagct actgccgcca
     8101 ttaagccgct aggtccgcct ccgataataa ttgtttgata catatacgct tcctccaaat
     8161 ttgttactgg acattcaatt gctattttat agtacttttt taaactttgc tatatctctt
     8221 gtgttatgag atgacaatta tgttgaatat atttataatg tgaacttcat aaaaaatgaa
     8281 gatgtatgtg tatgataaga atgatttttc tactgttatg cgatgttcat ctcaaatagt
     8341 agtataatgt ttatatgaaa tttcgtaata ttctaatcaa aatctagtat tcaatgacaa
     8401 tgtgttgata attttgttat gataattcat tgaatatacg acaatgaaat aaagttttgt
     8461 gatatattca taataatgaa aaatgatttt acagagaaac agcttttagt ttacaaaact
     8521 ttaataaaca tgagataggg agattcgtaa tgagtgaaag taaagaaatg gtgcgtggaa
     8581 cctttttaat taccattagt atattaatta ccaaggtatt gggcgtactt tttatcattc
     8641 cattcaacta tttaattggt ggacaagaaa atatggcgcc gttcacatac gcttatgcac
     8701 catataatat tgcaattgca gttgcgacag caggtgtacc attagcagct tcgaaatatg
     8761 ttgcgaaata taatgctatt ggggcatata aagtaagtca gaaattttat aaatcgagtt
     8821 ttattgtaat gagtattacc ggtgttttag gtttcttggt actgtatttc ttagcacctt
     8881 atatttctga attaacatta gctagaaaca ttcatgataa aaacggttgg tcagtcgatg
     8941 atattacttg gattataaga atcattagta tggttgttat ctttattcct gtattagcga
     9001 catggagagg gatattccaa ggttataaat ctatggggcc aacagcagtg tcagaagtaa
     9061 ctgagcaaat tgcacgcgtg attttcatat taatcggaag ttatttagta ctaaatgtct
     9121 ttgatggctc tattttatta gccaatggta ttgctacttt tgcagcagca gttggtgcga
     9181 ttattggtat attcacactc tggtattact ggagaaagcg taagcataat attgatcgta
     9241 tggtcgaatc tgattacaca gatatagatg tgtcatatgg caagatgtat aaagaaatta
     9301 ttgcttatag tataccgttc gttattgtaa gtttgaatta tccattattt aacttagtag
     9361 atcaatttac acataacggt gctttatctt tagttggtat accttcacag ttacaagata
     9421 tattctttaa tatgctaaat atgtcaacta ataaaattgt aatgattccg acatcattaa
     9481 gtgctggttt tgcagtgagt ttaattcctt atattacaaa aacatttgca gaaggtcgct
     9541 tacatgaaat gcatcatcaa attagaacat ctattggtgt attaatgttt ataactgttc
     9601 cagctagtat tggaattatg gccttggcac aaccattatt cacagttttc tatggttacg
     9661 atccaattgt tttaggtcat gaccctaacc atgatggtag tcgcttatta ttctattatg
     9721 cgcctgtagc aatactaatt tcactattaa gtgtaacggc atcaatgtta caaggtatag
     9781 ataaacagaa attaacagtt tatgttattt tagcttctgt agttatcaag ttagcgttaa
     9841 actatccatt aattatgtta ttccatacac ctggtgcaat tttaagtact agtatcgcat
     9901 tattatttgc aattggatgt aatttctata ttcttaaaaa atatgcgaag tttaaattca
     9961 gctatagttg gattcatttt gcaaaaatat tcttgtattc atttattatg atgctaggtg
    10021 tagaattagt atttttcctt gcaaatctat tcttagaacc tactaaactt ggttacttaa
    10081 tcattattat tttaggtgtt acagtaggta ttctaattta cggtacaatc acaattaaaa
    10141 cgcgtcttgc ggacgaattc ttaggtgaaa ttcctgaaaa attaagacgt agagttaggt
    10201 ttttacgatg agaatagata aatttttagc aaatatgggc gtcggtacac gtaatgaggt
    10261 caaacagtta cttaaaaaag gccttgtaaa tgtcaacgaa caagtaatta aatcaccaaa
    10321 aacacatata gaaccagaaa acgataagat aacagttcgt ggagaattaa tagaatacat
    10381 tgaaaatgtc tatattatgt taaataagcc aaaaggttat atatcagcaa ctgaagatca
    10441 tcattcaaaa accgttattg atttaatacc tgaataccaa catctaaata tttttccggt
    10501 tggtcgtctt gataaagata cagaagggct tttattgata acgaatgatg gtgattttaa
    10561 tcatgaatta atgagtccaa acaaacatgt ttctaaaaaa tatgaagtta tttcagcaaa
    10621 tcctatcact gaagacgaca ttcaagcttt taaagagggt gtaacactga ctgatggcaa
    10681 ggttaagcct gcaatattaa cttatattga taatcaaacg tcacacgtaa caatttatga
    10741 aggaaaatat catcaagtta aacgaatgtt tcatagcatt caaaatgagg tattacattt
    10801 aagacgtatc aaaattgctg atttagaatt ggacagcaat ttagattcag gagaatatcg
    10861 tttattaact gaaaatgatt ttgacaaatt aaattataaa taaaagggag acgattgata
    10921 tggcaaaagg taatttattt aaagcgattt taggtatagg tggcgctgta gcagctgtac
    10981 ttgttacacg taaagatagt cgtgacaagc tgaaagcaga atataataaa tataaacaag
    11041 atcctcaaag ctataaagat aatgctaagg ataaagcgac gcaattagga acaattgcta
    11101 atgaaacaat taaagaagta aaaacaaatc cgaaagaata tgctaataga ttaaaaaata
    11161 atccaaaagc atttttcaaa gaagaaaaat caaaatttac cgaatatgac aataagactg
    11221 acgaaagtat tgaaaaaggt aaatttgatg atgaaggtgg cgcagcacca aataataatt
    11281 tacgtatcgt cactgaagaa gatttaaaaa agaataaaaa tgcattatct gataaagaat
    11341 aagatgtgag taggacagaa atgataaggg agtgggacag aaatcatatt ttagcaaaat
    11401 ttatttcgtc gtcccacccc ggcaaggttg actagaattg aaaaaagctt gttacaagcg
    11461 tattttcatt cagtcaacta ctgccaatat aatattgtaa actataggac atttattagt
    11521 gtttcaggtt ctttatcgtt agtcatagct aatatgcata tagaaagaag aatacatgcg
    11581 tgaaactcat tcataagaaa tactaatatc taaagaaaac gtatttcttt atcttttcga
    11641 aattctctgt gttggggccc ttaccccaac ttgcacatta ttgaaagctg actattggcc
    11701 agcttctgtg ttggggccct taccccaact tgcacattat tgaaagctaa ctattggcca
    11761 gcttctatgt tggggccccg ccaacttgca ttgtctgtag aatttctttt cgaaattctc
    11821 tatgttgggg ccccggggcg cattttcgtt cggttaatta ctgtcaatat taaattgtgt
    11881 gcctagtaca ttgacttata tctcgagctc ttgaatatgt gtattttaaa tatataaatt
    11941 aaaaaaggaa caaagcagct atttatgatt tgtgtcatat caatagcatc gttttgttcc
    12001 tttttattat tttctgacat cttattgtag tttttatatc ttttcatgta aaatagaatt
    12061 taacttatag aaaaaaggaa gtcgatttcg aatgtggaaa gaaaaagttc aacaatacga
    12121 agatcaaatc attaatgact taaaaggatt attagcaatt gaaagtgtga gagatgatgc
    12181 aaaagcatca gaagacgcac cagttggtcc aggtcctcgt aaggcattag actacatgta
    12241 tgaaattgca catagagatg gatttacaac acatgatgtg gatcatattg caggaagaat
    12301 tgaggcaggt aaaggaaatg acgtattagg tatcttatgt catgttgacg ttgttcctgc
    12361 tggtgatgga tgggatagta atccgttcga gccggttgta acagaagatg ctatcatagc
    12421 tagaggtaca cttgatgaca aaggtccaac aattgctgct tattatgcaa ttaagatatt
    12481 agaagatatg aatgtggatt ggaagaaacg tattcatatg attattggta cggatgaaga
    12541 atctgattgg aaatgtacgg atcgctattt taaaacagaa gaaatgccaa cattaggttt
    12601 tgcaccagat gcagaatttc catgtattca tggtgaaaaa ggcattacaa catttgattt
    12661 agttcaaaat aaacttactg aagatcaaga tgaacctgat tatgaattaa taacttttaa
    12721 atctggtgaa cgttacaaca tggtacctga tcatgcagaa gcaagagtgc ttgttaaaga
    12781 aaatatgaca gatgttattc aagactttga gtacttttta gaacaaaatc atttacaagg
    12841 tgatagtact gttgatagtg gcattctagt tttaacagtt gaaggtaaag cggttcatgg
    12901 tatggatcca tctatcggtg tgaatgcggg tctttactta ctaaaattct tagcatcatt
    12961 aaatcttgat aataatgcac aagcgtttgt agcatttagt aatcgctact tatttaattc
    13021 agattttggt gaaaagatgg gaatgaaatt ccatacagat gtcatgggtg acgtgacaac
    13081 taacattggt gttattacat atgataatga aaacgcaggt cttttcggta tcaacttacg
    13141 ctacccagaa ggatttgaat ttgaaaaagc tatggatcgt tttgcaaatg agattcaaca
    13201 atatggcttt gaagtgaaat taggtaaagt ccaaccacca cattatgttg ataaaaatga
    13261 tccttttgta caaaagttag ttactgcata tagaaatcaa acaaatgata tgactgaacc
    13321 ttatactata ggtggcggta cttatgcgag aaacttagac aagggtgtag catttggcgc
    13381 aatgtttagt gattctgaag atttaatgca tcagaaaaat gaatatatca ctaaaaaaca
    13441 gttatttaac gcaactagta tttacttaga agcaatttat tcattatgcg tggaggaata
    13501 atatatggaa aaaatttttt taaatggtga gtttgtaagt ccaagtgaag caaaggtttc
    13561 atacaacgac agaggatacg tatttggcga tggtatttat gaatacattc gagtatataa
    13621 tggtaagtta tttacagtaa cagaacatta tgaaagattt ttacgtagtg ccaatgagat
    13681 tggtttagat ttaaattatt ctgtagaaga attaattgaa ctatctcgta aattagttga
    13741 tatgaatcaa attgaaactg gggcaattta tattcaagca acgcgtggtg tagctgaaag
    13801 gaatcatagc ttcccgacac ctgaagtaga accagcaatt gttgcttata caaagagtta
    13861 tgatcgtcct tatgatcatt tagaaaatgg tgtgaatggt gttaccgttg aagatatccg
    13921 atggttacgt tgcgacatta aaagcttgaa cttattagga aatgtattag caaaagaata
    13981 tgctgtgaaa tataatgcag ttgaagcaat tcaacatcga ggtgaaactg taactgaagg
    14041 atcttcaagt aatgcttatg caattaaaga cggtgtgatt tatacacatc cgattaacaa
    14101 ctatattctt aatggtatta cacgaattgt aattaaaaaa attgccgaag actataacat
    14161 cccatttaaa gaagaaacgt ttactgtaga tttcttgaaa aacgcagatg aagttattgt
    14221 ttcaagtact tcagctgagg ttacacctgt tattaaatta gatggtgaac cagttaatga
    14281 tggtaaagtt ggcccaatta cacgtcaact acaagaagga tttgaaaagt atatagagtc
    14341 acacagtatt taaaattctt tcatcatatt tttagattaa attacttaaa tttatcaact
    14401 ttcgtaagtg aattatgtta taatttaaaa cgaagcttaa aagtgttgtt tagaaaacac
    14461 atattagtaa aaagtgccat tttgtttctc attttcagtt gaaatataat gataaaaatt
    14521 ataaaatttt ctatgagtta caaatatgat gtgcatcata ttttactaag tacgtaaatg
    14581 ttacagttca taaatttatg ctaaccatga aaatgggata gcaattattt tgaaaactag
    14641 agtttatcgc ctacagagat tcaaaaacat gtagttttag acacggtaac attatatgtc
    14701 aagtaagtga ctatagaatg tatggtgtca atgacagaca ttacgattta aatttagtcg
    14761 tgtaatgtag gcattggttg aggatttaca ataattatct ggacataata tttgtaccag
    14821 tatggtgtta taaattagaa tatatggctc ttgaaaaacc tatttctaga gccattttct
    14881 aattgaaagt gaggtggacg agttggagca gttttatcaa ttagggtgga cacttgattc
    14941 agcaggtggt gcatctggtg aagcatatat ggctgaacaa gatggacaaa agttgttttt
    15001 aaaacgaaat tcaaatccat ttattgcggc attatcagca gaaggtattg tgcccaaatt
    15061 agtatggacg aaacgcatag aaacaggcga ggttgttaca gcacaacatt ggaaaaatgg
    15121 gcgtgaacta tcttcaaacg aaatgaagca aacaagagtt gcacatttat taaagaagat
    15181 acacaattct agacctttat taagtatgtt aaagcgtatg gaaatggaac ctattactcc
    15241 tgagattatg cttaataaaa ttaatgcctc tttatcaaga gaagttttaa cacatcatat
    15301 tgtgagaaaa tcattaacct atttagaaga gcatataccg agtttagatt cgcgtttttt
    15361 cactgttgta catggtgatg tgaatcataa taattggtta ttatccgatc gagatgaact
    15421 atttttagtt gattgggaag gtgcaatgat tgcagacccg gcaattgata tcggaatgct
    15481 gctttacaac tatgttccac aacaacaatg gtctgaatgg cttgaaacat atggtgttca
    15541 agagagcttg aatttaaata aacgtatgaa atggtatacg gtaattcaat ctataggtct
    15601 tgttcaatgg tatgaagagc aaaaaagata taaggatatg aacacttggc ttaaattcct
    15661 aaatgaagtt atgaatagta acatgtttat ttaagaggag aataaaatat gagagttcga
    15721 tacaaaccat gggctgaaga ttatttaaaa gatcatcctg aattggttga catggacggt
    15781 caacatgcag ggaaaatgac tgaatggttt gataaaacgc aaccgataca cattgaaatt
    15841 ggttcgggta tggggcagtt tattacaaca ttagctgctc aaaatcctca tattaactat
    15901 atttcaatgg aacgtgagaa aagtatcgtt tataaagtat tggataaagt taaagagatg
    15961 ggtttaacaa atttaaagat tatttgtaat gacgccattg aattatatga atattttaaa
    16021 gacggcgaag tttcgcgtat ttatttgaac ttttcagatc catggccaaa aaatcgtcat
    16081 gcaaaacgac gtttaacgta tcatacgttt ttagcgttgt atcaacaaat cttaaatgat
    16141 gaaggagatt tacattttaa aactgataac agaggactat ttgcctacag tttagaaagt
    16201 atgtctcaat ttggaatgta ttttacaaaa ataaatctaa atttacatca agaagacgat
    16261 ggcagtaata ttttaacaga atatgaaaag aaattttcgg ataaagggtc acgtatttat
    16321 cgtatggaag cgaaattcca ttcacaaaaa tagagcactt atgagggtat gtacacatac
    16381 aatcataagt gttttttcgt actataatgg ttctatagat aaaggttcta taataaatcg
    16441 gactgatgga aaaagttttt aacttttcat ctgtccgatt ttttgatttt gaatata
//
LOCUS       DANJFZ010000055        16402 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.055, whole genome shotgun sequence.
ACCESSION   DANJFZ010000055 DANJFZ010000000
VERSION     DANJFZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.055"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(197..415)
                     /locus_tag="PWN45_001556"
     CDS             complement(197..415)
                     /locus_tag="PWN45_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDL0623325.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            582..1460
                     /locus_tag="PWN45_001557"
     CDS             582..1460
                     /locus_tag="PWN45_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0623326.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            1673..2068
                     /gene="cidA"
                     /locus_tag="PWN45_001558"
     CDS             1673..2068
                     /gene="cidA"
                     /locus_tag="PWN45_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDL0623327.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            2061..2750
                     /locus_tag="PWN45_001559"
     CDS             2061..2750
                     /locus_tag="PWN45_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL0623328.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            2797..4536
                     /locus_tag="PWN45_001560"
     CDS             2797..4536
                     /locus_tag="PWN45_001560"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL0623329.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            4837..6903
                     /locus_tag="PWN45_001561"
     CDS             4837..6903
                     /locus_tag="PWN45_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDL0623330.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            7283..7693
                     /locus_tag="PWN45_001562"
     CDS             7283..7693
                     /locus_tag="PWN45_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0623331.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            7735..8079
                     /locus_tag="PWN45_001563"
     CDS             7735..8079
                     /locus_tag="PWN45_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0623332.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            8212..9180
                     /locus_tag="PWN45_001564"
     CDS             8212..9180
                     /locus_tag="PWN45_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623333.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(9262..10197)
                     /locus_tag="PWN45_001565"
     CDS             complement(9262..10197)
                     /locus_tag="PWN45_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL0623334.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(10380..10583)
                     /locus_tag="PWN45_001566"
     CDS             complement(10380..10583)
                     /locus_tag="PWN45_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623335.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            11134..12177
                     /locus_tag="PWN45_001567"
     CDS             11134..12177
                     /locus_tag="PWN45_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL0623336.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            12180..12860
                     /gene="sdaAB"
                     /locus_tag="PWN45_001568"
     CDS             12180..12860
                     /gene="sdaAB"
                     /locus_tag="PWN45_001568"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL0623337.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            12874..13773
                     /gene="sdaAA"
                     /locus_tag="PWN45_001569"
     CDS             12874..13773
                     /gene="sdaAA"
                     /locus_tag="PWN45_001569"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL0623338.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            13792..13890
                     /locus_tag="PWN45_001570"
     CDS             13792..13890
                     /locus_tag="PWN45_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623339.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            13952..14122
                     /locus_tag="PWN45_001571"
     CDS             13952..14122
                     /locus_tag="PWN45_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623340.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(14560..15051)
                     /locus_tag="PWN45_001572"
     CDS             complement(14560..15051)
                     /locus_tag="PWN45_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0623341.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            15245..15865
                     /gene="srtA"
                     /locus_tag="PWN45_001573"
     CDS             15245..15865
                     /gene="srtA"
                     /locus_tag="PWN45_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDL0623342.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            complement(16025..>16402)
                     /locus_tag="PWN45_001574"
     CDS             complement(16025..>16402)
                     /locus_tag="PWN45_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDL0623343.1"
                     /translation="SIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISLGI
                     SIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVSFF
                     VLLFIVNTIVLLLSRKRWLKIGM"
BASE COUNT         5751 a         2468 c         3134 g         5049 t
ORIGIN      
        1 gtgatacaag gatgtcagga ttgattaaca gcggatatta gtgggaaaat ataaaatgat
       61 ttaaaaaaaa ggtacatggt tctataactg aaagaaaata gcaatcacaa tatactaaat
      121 gtagcaataa cattaaaaat agcgccctgc aatcagagcg ctaacatgag atgatgtgac
      181 tccatgtatt catgtatcat tgtacatttg tgaattggtt gaaaactgat agtgcccgtt
      241 taattacagg tactgagatg ctttcaggac atggatttgt tcgaaaatat gacaatattt
      301 cactatggct cttcacttct ctagggaaat tgctatcttg attaatccaa tcaaccaatt
      361 cacctaatgg tgtgtcatca ccaagaaaac tttgcataaa ttcgtaaaag ctcaatacct
      421 cacctcgatt aatatacttg agccattaat ataataccat aaaagaatgt gtattttaca
      481 taaaactttt tataaagcaa ttttataaat ctaaatttat tgaatgttta agaagttgtg
      541 tataatgaaa taaacgtgtt gaaatagtta ggatgatgtt agtggatatc aaacatatga
      601 aatattttat tgaagtcgtt aagcaaggag gcatgactaa tgcttccaaa tcattatata
      661 ttgcacaacc tacaattagt aaagcaatta aagatattga aaatgaaatg ggcacgccct
      721 tatttgatag aagtaaaaga catttaattc ttaccgatgc aggtcaaatt ttttatgaga
      781 aaagtaaaga aattgttgca ctgtatgatt atttaccatc tgaaatggaa cgcttgaatg
      841 gactggaaac aggacatata aacatgggca tgtcggcagt catgaatatg aagattctta
      901 tcaatattct tggtgcattc catcaacaat atccaaatgt tacatataat ttgatagaaa
      961 atggcggtaa aacaattgaa cagcaaatta tcaatgatga agtagatata ggcgtgacca
     1021 ctttgccagt cgatcatcat attttcgatt atactaccct agataaggaa gatttgcgac
     1081 ttatcgtgag cagagagcat cgactcgcaa aatatgaaac tgttaaactc gaagatttag
     1141 caggtgaaga cttcatttta tttaataaag acttttactt gaatgataaa attattgaaa
     1201 atgctaaaaa cgttggcttt gttccgaata ctgtagcgca aatttcacaa tggcatgtta
     1261 tagaagattt agttacgaat gaattaggta ttagtatttt accaacatca atttcagagc
     1321 aactaaatgg agatgtgaag ctgctacgca ttgaagatgc tcatgtacat tgggaattag
     1381 gtgttgtttg gaagaaggat aaacaattaa gtcatgccac aacgaaatgg atagaatttt
     1441 tgaaagaccg tttaggctaa catattaata aagcactcat tatttaaggc gcatcattac
     1501 gtgggtcatt gaaataatga gtgttttttt gtgaaaatga agtgaaattt agagagcgtt
     1561 tccatagaaa atagtaatac aaactataaa aaaagagtat ttttatattg tgtacgccat
     1621 ctttataata gttattgtaa caatttagac atatttagaa agggatggcg ccatgcacaa
     1681 agtccaatta ataatcaaac tactactaca actaggaatc atcattgtga ttacttatat
     1741 tggcacagaa attcaaaaga tttttcatct tcccttagcc ggcagtattg ttggtctatt
     1801 tttattttat ttactattac aatttaagat tgtaccgcta acttgggtag aagacggtgc
     1861 aaacttttta ttaaagacga tggtcttttt cttcataccg tcagttgtag gtattatgga
     1921 tgttgcttcc gaaattacgc taaattatat actctttttc gcagtcatta tcataggaac
     1981 atgtatcgtt gcattatctt caggttatat tgctgaaaaa atgtctgtta aacataaaca
     2041 tcgtaaaggt gtagacgctt atgaatgatt acgtgcaagc cttattaatg attttgttga
     2101 ctgtcgtttt atattatttc gctaaaaggt tacaacaaaa atatccgaac ccatttttga
     2161 atccagcatt aattgcatct ttaggaatta tttttgtctt acttatcttt ggaattagtt
     2221 ataacgggta tatgaaaggt ggcagttgga tcaaccatat tttaaacgca acggtcgtat
     2281 gtttagcgta cccactttat aaaaatagag agaaaattaa agacaatgtc tctatcattt
     2341 ttgcaagtgt attaactggc gtcatgctga atttcatgtt agtgttctta acacttaaag
     2401 catttggcta ttctaaagac gtcattgtaa cgttattgcc ccgatctata acagccgcag
     2461 taggtatcga agtgtcacat gaactaggtg gtacagatac gatgaccgta ctttttatta
     2521 tcacaacggg tttaatcggt agtattttag gttcgatgtt attaagattt ggaagatttg
     2581 aatcttctat cgccaaagga ttaacgtatg ggaatgcgtc acatgcattt ggcacagcta
     2641 aagcactaga aatggatatt gaatccggtg catttagttc aattgggatg attttaactg
     2701 cagttattag ttcagtgtta atacctgttc taattttatt attctattaa tttagatatt
     2761 taaaatgata gacagaaagg gaggctatta gtaataatgg caaaaataaa agcaaatgaa
     2821 gcattagtta aagcattaca agcatgggat atagatcact tgtatggtat tccaggagac
     2881 tcaatcgacg cagtagtcga tagtttacgt acagtgagag atcaatttaa attttatcat
     2941 gtacgtcatg aagaagtagc aagcttagcg gctgctggtt acacaaaatt aactggtaaa
     3001 atcggtgtgg cattaagtat cggtggccct ggtttaattc atttattaaa tggtatgtat
     3061 gatgccaaaa tggataatgt accgcaatta atattatctg gacaaacgaa tagtacagca
     3121 cttggaacga aagcattcca agaaacaaat ttacaaaaat tatgtgaaga tgtagccgtt
     3181 tataatcacc aaattgaaaa aggtgacaat gtgtttgaaa tcgttaacga agcaattcgt
     3241 acggcatatg aacaaaaagg tgtagctgtt gttatttgtc ctaacgactt attaactgaa
     3301 aaaattaaag atacaacgaa taaaccagta gatacatcaa gaccaacagt agtatcacca
     3361 aaatataaag acatcaaaaa agcggttaaa ctaattaata aaagtaaaaa gcctgtcatg
     3421 ttaattggtg taggtgcgaa acatgcgaaa gatgagctac gtgaatttat tgaaatggct
     3481 aaaattcctg tcattcattc attaccagct aaaacaatct tgccggatga tcatccatat
     3541 agtatcggta acttaggtaa aatcggtacc aaaacatctt atcaaacaat gcaggaagcg
     3601 gatttattaa ttatggttgg tacaaactat ccatatgtgg attacttacc taagaaaaat
     3661 attaaagcca ttcaaattga cacaaatcct aaaaatatcg gacatcgttt caatattaat
     3721 gtaggaattg ttggagatag taaaattgcg ttgcatcagt taactgaaaa tattaaacat
     3781 gttgctgaaa gaccattctt aaacaaaacg ttagaacgta aagcggtttg ggataaatgg
     3841 atggaacaag ataaaaataa taatagtaaa ccattacgtc cagaacgatt aatggcatca
     3901 atcaataaat ttattaaaga tgatgcagtg atttcagcag atgtaggtac agcaacagtt
     3961 tggtcaactc gatacttaaa ccttggtgta aataacaagt tcatcatttc aagttggtta
     4021 ggtacaatgg gttgcggtct tccaggtgca attgcatcaa aaattgcata tccaaataga
     4081 caagccatcg caattgctgg tgacggtgca ttccaaatgg taatgcaaga cttcgctaca
     4141 gcagtacaat atgatttacc tttaactgta tttgtactta ataacaaaca gttagcattt
     4201 attaaatatg aacaacaagc agctggtgaa ttagaatatg cagttgattt ttctgatatg
     4261 gatcatgcaa aatttgctga ggcagcaggt ggtaaaggtt atacaattaa gagtgctagc
     4321 gaagtagatg ctatagtcga agaggcatta gcacaagatg taccaacgat tgtagatgta
     4381 tatgttgatc ctaatgctgc gccattacca ggtaaaattg taaatgaaga agcgcttggt
     4441 tatggtaagt gggcatttag atcaattact gaagataaac atttagattt agatcaaatt
     4501 ccaccaattt cagtggcagc aaaacgtttc ttataactga tttaaaggtt atcacaattg
     4561 aattgaacta taaaaacggt aatttctatt tcaacaaaat gggaattgcc gttttgttta
     4621 tttatcacaa atgatcgtac tgaattgatg ataaaattgt gaaaaagttg ttgaaaacgc
     4681 ttttacaaat atgtataata gctatgaatt agatatcact tgcgtgttac tggtaatgca
     4741 ggcatgagca aacaaccgca ctatgagaat agtcttgttt gttcatgcct gctttttttg
     4801 tacatggaag cggaaattga gataggggat gtttatatgt ttaagaaatt gtttggacaa
     4861 ttgcaacgta tcggtaaagc attaatgtta cctgttgcga ttttaccagc agctggtatt
     4921 ttattagcgt ttggtaacgc aatgcacaac gaacaattag tagaaattgc accatggtta
     4981 aaaaacgata tcattgtaat gatttcgtcg gtcatggaag cagcaggaca agttgtattt
     5041 gataacttgc cattattatt tgcagttggt acagcacttg gattagcagg aggagacggt
     5101 gttgcagcat tagcagcgct agtaggttac ttaattatga atgcaacaat ggggaaagtg
     5161 ttgcacatta caattgatga cattttctca tatgccaaag gggcaaaaga attaagtcaa
     5221 gcagcgaaag aaccagcaca tgctttagta ttaggtattc caacgttaca aacgggtgtg
     5281 tttggtggta ttatcatggg tgctttagcc gcatggtgtt acaacaaatt ttataatatt
     5341 acactaccac catttttagg attctttgca ggtaaacgat ttgtaccgat tgtgacatcg
     5401 gtcgtagcaa tcgcaacagg tgtgctttta agctttgcgt ggccaccaat tcaagatgga
     5461 ttaaatagtt tatcgaattt cttattaaat aaaaatttaa cattaacaac gtttatattc
     5521 ggtattattg aacgctcatt aattccattt ggtttacatc atattttcta ttcaccgttc
     5581 tggtttgaat tcggaagtta tacaaatcac gcaggtgaat tggttcgtgg tgaccaacgt
     5641 atttggatgg cacaattgaa agatggcgta ccatttactg ctggtgcatt tactactggt
     5701 aaatatccat ttatgatgtt tggtttacca gcggcggcat ttgctattta taaaaatgca
     5761 cgaccagaac gtaaaaaagt cgtgggtggt ttaatgttat cagcaggatt aactgcattt
     5821 ttaactggta tcactgagcc attagaattt tcattcttat ttgtagcacc agtactttat
     5881 ggaattcacg tattattagc tggtacatca ttcttagtaa tgcatttatt aggcgttaaa
     5941 attggtatga cattctcagg tggtttcata gattatattt tatatggttt attaaactgg
     6001 gatcgttcac acgcattatt agttattcca gtcggtattg tatatgctat cgtgtattac
     6061 ttcttattcg actttgcaat tcgtaagttt aaattgaaaa caccaggtcg tgaagatgaa
     6121 gaaactgaaa ttcgtaactc tagtgtcgca aaattaccat ttgatgtctt agatgcaatg
     6181 ggtggaaaag aaaacattaa acatttagat gcatgtatta cacgtctacg cgtagaagtg
     6241 gttgataaat caaaagtaga tgtagcaggt attaaagctt taggcgcatc aggtgtatta
     6301 gaagttggaa acaatatgca agctatcttt ggtccaaaat cagatcaaat taaacatgat
     6361 atggccaaga ttatgagtgg tgaaattacg aaaccaagtg aaacgacagt gactgaagaa
     6421 atgtcagatg aaccagttca cgtagaagca cttggaacaa cagacatcta tgcaccaggt
     6481 atcggtcaaa tcattccatt atcagaagta cctgatcaag tgttcgctgg taaaatgatg
     6541 ggtgatggtg ttggctttat ccctgaaaaa ggtgaaattg tagcaccgtt tgatggtaca
     6601 gtgaaaacaa tcttccctac gaaacatgcg ataggattag aatctgaaag tggcgtcgaa
     6661 gtacttattc atattggtat cgatacagtg aaactgaatg gtgaaggatt cgaaagtctg
     6721 attaacgttg atgaaaaagt aacacaaggt caaccattaa tgaaagtgaa tttagcatac
     6781 ttgaaagcac acgcaccaag catcgttaca ccaatgatta ttacaaatct tgaaaataaa
     6841 gaacttgtca ttgaagatgt acaagatgct gatccaggta agctaattat gacagtcaaa
     6901 taatgattaa aaatgaaaca gcatatcaaa tgaatgaact tttagtcatt cgtagtgcgt
     6961 atgcgaagta gcgagttgaa agagaatacg ttacaaaagg cagtagctta aaatgaagct
     7021 actgcctttt tagtgcgcaa tgatgtatag caggtgtgtt gatgataata agttaaatat
     7081 tagtgttaga tatagaaaac attgcttatg tttttgtcac attttagaaa aatgcatctt
     7141 cgcgactagc caaattaata gtctcattga aaagtttgat tttccgtaat aaagttcaat
     7201 tgcttgtatt tttatcatga aagtaataaa aataaattaa catgatttta aatctatttg
     7261 taagataagg agatttgtca ttatgacaac agaaggtcta ttagttgcag agaaagaaat
     7321 cgaagtgaat ggttacgaca ttgatgcgat gggtgtcgtt agtaatatcg tttatattag
     7381 atggttcgaa gatttgagaa cagcgtttat taatcagcac atgaattact caacaatgat
     7441 caatcaaggc atttcaccta tacttatgaa aacggaagca gagtataaag tacctgtcac
     7501 aatacatgac aaaccagtag gtcgtattta cttagttaaa gcaagcaaga tgaaatgggt
     7561 gtttcagttt gaaattgtgt ccgcacatgg cgtgcattgt attggtacac agacaggcgg
     7621 tttttacaga ttgagtgata agaagataac ctctgtgcca caagtgtttc aagacatttt
     7681 agcaacaaaa taatgacttc attttaaaat ataaaaagta agaaggtgtt cgaaatggtt
     7741 aagcaattaa atagtgtcga agcattccgt gaatttattc atcaatatcc gttagcagtt
     7801 gtacatgtca tgcgcgatca gtgtagcgtg tgtcatgccg ttttaccaca aattgaagac
     7861 ttgatgcaat catatcccaa tgtgccatta gctgtgatta atcaaagtca ggtggaagct
     7921 attgctggag aattaaatat tttcactgta cctgtggatt taatttttat gaatggaaaa
     7981 gaaatgcatc gtcaagggcg ttttatcgat atgcaacgtt ttgaacatca tcttaagcaa
     8041 atgaatgata gtgtaaataa cgatgtcgat gagcattaat atcgcaaatg attagcattg
     8101 ctaagattat gtagacatca taacttattt cccagtaaat attggtagta attagaatca
     8161 gcatggtaca gtagaactat agtagaaatc atcaaagagg agtgacgaca aatgcgtaaa
     8221 aaatggtcta cacttgcgtt tggattttta gttgcagcat acgcacatat tagaattaaa
     8281 gaaaaacgca gtgtgaaaag ttatatgtta gaacaaggta tacgattatc tagagctaag
     8341 cgtcgtttta tgtataaaga agaagcgatg aaagcattag aaaaaatggc gccacagaca
     8401 gcaggcgaat atgagggaac caattatcag tttaagatgc cagtaaaagt ggataagcac
     8461 ttcggttcaa ccgtttatac cgttaacgat aaacaagata agcatcaacg cgttgtatta
     8521 tatgcacatg gaggcgcatg gttccaagac ccactcaaaa ttcatttcga atttattgat
     8581 gaacttgcag aaacactcaa tgctaaagtc atcatgccag tatatccgaa gattccgcat
     8641 caagattatc aagcgacgta tgtgcttttt gaaaagttgt accatgattt attgaatcaa
     8701 gtagcagatt ctaaacaaat cgttgtaatg ggtgactctg cgggcggtca aattgcttta
     8761 tcatttgctc aattgttaaa agaaaaacat attgtgcaac caggacatat tgtattaatt
     8821 tcaccagttt tagatgcaac gatgcagcat cctgaaattc ctgactactt aaagaaagac
     8881 ccaatggtag gtgtggatgg cagtgtgttc ttagctgaac aatgggcagg ggacacacct
     8941 ttagataact acaaagtatc accaattaat ggtgacttag atggtttagg tcgtattaca
     9001 ttaactgttg gtacaaaaga agtactgtac ccagatgctt tgaacttatc gcaattgttg
     9061 agtgcgaaag gtatcgaaca tgactttata cctggatatt accaattcca tatttatcca
     9121 gtatttccga taccagagcg acgacgcttt ttataccaag tgaaaaatat tattaactaa
     9181 aataaaaaac tctgatatca cctataaggt aactacgttg ttaaataata gttacttgct
     9241 aaagtgatta tcagagtttt cttattcatc taatgtttca ggttgtcttt taagtgatag
     9301 taataaaatt aaaatcataa taagaatgat gcctacgatt tgaaatggtt tgaatgccac
     9361 atgtaaccat agactgcttg cgataacggc tacgacaggt tcaacagttc cgaataatgt
     9421 tgtttctttc gctgatatgt attgtaaact gtcgataaag aaataaaatg cgagtgcggt
     9481 accaccgata ataccaaaga ttaaaaataa tataattgat atgtcccatt tagtgatatc
     9541 aatgtgccaa attggatggc gtaaattcat cgcaacacca gaaataagca ttgcccaccc
     9601 gacaattaga atcgaaccga agcggttaag taggtctgaa ggataaattg tgtaaaaagc
     9661 gagtgctaca ccagctaaaa taccccagaa taaacttgca ggattgacga ctaaattaga
     9721 aaatgaacca tttgttaata ataaaaatgt tcctaatagt gtcatgataa tagcaagcac
     9781 atcaaataat gttagttttg caacgcctct tatgacaaac caaataataa tataaactgg
     9841 cgcaatgtat tgtagtaatg ttgcaatcgc agcattacct gtattaatag atgccatata
     9901 agcatattgt actactaaca tgcccagtat actgaagata agtaatttgc ctaacatacg
     9961 tcgatcttgg aatacgctaa atattgaacg tttgggttgc atcattttgt acataataag
    10021 taagaaaaca ccactgacta caagtcgagc agtgacgtac caatcgacat taatattctt
    10081 atatttgaac aagaaatcag aaacagtacc acctaatccc cataaacaag caccgacaat
    10141 ggcaagtata attcctttta aacgcaaagc tgaatgatta ttcgccaagt atctcacccc
    10201 tttaaaactt aacaccaaca acataaaata tgtcggtgta tgataatgat ttaattcatt
    10261 atatcgactt ttaagaaaga gtgaaatgag aaaataaaaa aatcatctaa gatgtagtgg
    10321 tctagatgat agataaatga atcgatatgg gaaatgaatg ttgaaacaca gaggataaac
    10381 tatttaaatg agatttcagg aagactcaaa tgtttttgcg taagagatcc atgatgagaa
    10441 tgctgactat ctaacacgaa atgtgataca tcatgtccag tcatactcgt atataaaata
    10501 atgactaaat aagaagtgaa cgttataaag ataagtgcta aatagataga aattgcaact
    10561 ttaacggaat gactcaattg cacctttatc gcctccacta agttatacta aatattttca
    10621 tcttcagtat aaccaaccat tgttaagttg atgtaaattt agctttaaaa aaaagtaaag
    10681 attcaaccta tagactatca tcattcctat cagaaaacta aaaataatat cctactttaa
    10741 ttatcttatt gcaaatatat gtgtaataag ttatttttga atatagtgtg cggttttata
    10801 aacacagcac aaaaacaaaa aataaatagc tcgaaagata aagtgtataa atattaaaag
    10861 tttttagaaa tattacttac tttcagatgt aaaaaagtta tcaaaaaact gtcacacaac
    10921 tgtttgaaat atagagtaaa aagttaatta gtaaatatta acgacaacat aagtagtttt
    10981 tgaaacaatc cattttaaaa atctttcaca aatatcaaat atttcactta aatatttgat
    11041 ttgataacag ataaaagttg aaaacgtttt cttaagggtg tacgatgttt atggtctgaa
    11101 aataaaataa acatagagag aggtttttgg gaaatggata ttctgttagg tgtaggcaca
    11161 ttagtcttgg ttttagtgat catgacgcta tttttaaagt atgcaccgta cggtaaacaa
    11221 ggactacaag cattatccgg tgcagcttgt gcgacatttc taccacaagc attcttaagt
    11281 tatgctattg gtggcgtatt tgatattaaa tttctacaag atattgggga tttagcagga
    11341 agtttaagcg gtatcgcagt aggtattcta acgtgcttaa acttaggcgt tgcaccagta
    11401 tttgcggtca ttgttggttt agtattacat gactttaaat tattaccagc attcatcgca
    11461 gcttatggcg tagctttttt aattaaatgg attgaaaaga aagttccaga aggcttggac
    11521 ttaattgtgg tgatcttatt tgcaccagca attgctttcg gacttgcatc aatcattaca
    11581 cctggtgtgc tagcaacatt aaaacaaata ggtagtgctg tcactgcggt aggtgataac
    11641 aatccatatg cattagcggt cattttagga ctagtgattc ctgttacagg tatgacaccg
    11701 ctaagttcaa tggtattaac aagtttatta ggactgacag gtgttcctat ggctattgga
    11761 gcactaacat gtacaggtag ctcatttgtt aaccttattt tattccgaaa attaaacatt
    11821 ggtggaccgt caaaagcatt tgcagtttgt attgagccat tgacgcaaat tgatttaata
    11881 gcgcaatatc cagtacaact atatggtaca aatgcactga ttggggtcgt caacgcatgt
    11941 atcgtgacat ttagcggact agtaataggt gtcaaaggta tggcaacacc aattgcaggc
    12001 gccatcgtat tgtttggctt taataatgca gtgacatcaa ttgtaacaat cgcaacagtg
    12061 attattgtca gcattgtctt agcctatatt attggaacac taattaataa gtttaattta
    12121 atgaatatca actttaaaat gccgagcaaa aagaatcata ttaaggagag tgtttaatta
    12181 tggcaaagag ctatgattat caaagtgctt tcgatattat cggaccagtt atgatgggcc
    12241 cttcaagttc tcatacggct ggtgcagtaa agattgggaa ttcagcacgc gcaatattgg
    12301 gagatattcc taaaaatatt gaaatacatt attatgaatc atttgctcaa acacaccaag
    12361 gacatggtac agatgtcgct attgtgggtg gagcaatggg atattcaaca tttgataacc
    12421 gaattaaatc ttcattagaa attgcagctg atgatggtat taaaatagat atcatcgaag
    12481 aagaaggcga tagtattggt caacatccaa actgtgctta tatcaaatca tctcgtaatg
    12541 atggtcgcta cattgaaatt atcggtattt caatcggtgg tggtacaatt aaaattaaag
    12601 gtattcatat aaatggacta gaagtagatt taaatcatgg attaccaatt ttagttgttg
    12661 acggaaatat gactaaagca caagtaaatc atttcatcaa tgatatcaac gatatgaaat
    12721 tagactgtaa agatgaattt attaagatag atgaagataa atgcttggtt gtaataccat
    12781 taaataaagc aatctcagaa tcaacattaa atacaattaa agagaaatac agtgacgtaa
    12841 acgtttccta tataaattag agggggaata aacatgtttg attcaattag agagactata
    12901 gattatgccg tagaaaataa tatgtcattt gcggatatca tggttaaaga agaaatggaa
    12961 ttaagcggta aatcacgtga tgaagtgcga gcgcaaatga aacaaaattt agatgtcatg
    13021 cgagacgcag taatcaaagg gacgacaggt gatggggttg aaagtgtaac gggctacact
    13081 ggtcatgatg ctgctaaact acgtgattat aatgaaacac atcatgcttt gtctggatat
    13141 gaaatgattg acgcagtcaa aggtgccatt gcaacaaatg aagtcaatgc tgcgatgggt
    13201 attatttgtg caacgccaac agctggttcc tcgggtacca ttcccggtgc actttttaaa
    13261 ttagaaaaaa cacatgattt aacagaagag caaatgattg atttcttatt cacttcagca
    13321 ttgtttgggc gtgtcgtagc aaacaatgca agtgtagctg gtgcaacagg tggctgccaa
    13381 gctgaagttg gttcagcatc tgcaatggcc gcagctgcag cagtagctat attcggagga
    13441 tcaccagaag catccgggca cgctatggca ttagcgataa gtaatttatt aggtttagtt
    13501 tgtgatccag tagccggact tgttgaaatt ccttgtgtta tgagaaatgc aattggttcg
    13561 ggtaacgctt taatttcagc agatttagca ttagcaggta ttgaaagtag aatccctgtt
    13621 gatgaagtta ttgaagcaat ggataaggtt ggtcgtaacc ttcctgcatc attacgtgaa
    13681 actggattgg gtggactagc aggcacacca actggcgaag caattaaacg taaaatcttt
    13741 ggcacagctg aagatatggt taaaaataat taatgagata acaataaaat attgataaaa
    13801 aagaagcatc tgtctggatc attagtacca gatagatgct tcttaattat ctctgaagaa
    13861 gttgaaagat ttatggaaaa tgcatactaa tcaaaatagt gattgcaaaa gcgcccccgt
    13921 atatctaaaa taacttaaaa ggagtgatga tatgctaaga ctatcaatca tctttatagc
    13981 attcatcatt aacacaacga ttacatatgg atatacgact gagggaacgt gggtcaatct
    14041 attatttaaa tctttatcgc tcagcatgat tatcgttttc atgttctact atattcgttt
    14101 tgttattgag aaaaagcgtt aaatcaaagc tatggggata gtatttgaaa agaataaata
    14161 aaagggacaa tgtaaatcaa tgtaatatgc tctacaaagt tatattggca gtagttgact
    14221 gaacgaaaat gcgcttgtaa caagcttttt gggccccaac acagaagctg acgaaaagtc
    14281 agcttacaat aatgtgcgag ttggggtggg acgacgataa agaaatactt tttctataga
    14341 aattagtatt tcttatgcat gagttttact catgtattcc tatttttaag tacacattag
    14401 ctgtggctaa tgtgtaagaa ccactacata ataaatcatt agtggctctt tatcatttct
    14461 gtcccactcc cctgagaagt ttaaaatttt atatgttggc ttgttatgtt aagggaatta
    14521 acatggttgt cttgtttata ttatgtgatt caaacattac tagtcttgta aatctaattc
    14581 gtaaaatgct aaatctaacc atctattaaa tttaaaacct acattggtca gtgtgccggc
    14641 atgcttaaaa gcaaactttt gatgcaactg aatactcgct ttgttggaag catcaatgcc
    14701 tgcaactagc gcacgataac ctttagcttt tgcttccaca attaaatggt gtagtaattg
    14761 actagcaata ccttttcctc tagctgaagc atcgacataa atagaatgtt cgattgtata
    14821 taggtatgct ggccaaggtc taaatgaacc gaacgtcgca aaccctaaga cacttccatt
    14881 ttcctcaaat acaaagatag gctcatgctt acgttgtttc gtttcaaacc atgcgacacg
    14941 ttcgtctatg gtttgtggtt cataagtata aacagctgta gtattgataa tggcatcatt
    15001 gtatatcgct aatatagcgt ttaaatcctc ttttttagcg tatctaatca tatcaattcc
    15061 cccttagtaa ttattaaaag cgtttcgtta tttgaatgca aatatgtgta atgaaatcta
    15121 acgtaaaagt atacatgtaa attttatagt ataaaatgaa ttgctatgag tcattttgaa
    15181 attaatggta tactatatga aatgttaaca ggcattgtga aatgtataaa aggagcctta
    15241 acgtatgaaa aaatggacaa atcgattaat gacaatcgct ggtgtggtac ttatcctagt
    15301 ggcagcatat ttgtttgcta aaccacatat cgataattat cttcacgata aagataaaga
    15361 tgaaaagatt gaacaatatg ataaaaatgt aaaagaacag gcgagtaaag ataaaaagca
    15421 gcaagctaaa cctcaaattc cgaaagataa atcgaaagtg gcaggctata ttgaaattcc
    15481 agatgctgat attaaagaac cagtatatcc aggaccagca acacctgaac aattaaatag
    15541 aggtgtaagc tttgcagaag aaaatgaatc actagatgat caaaatattt caattgcagg
    15601 acacactttc attgaccgtc cgaactatca atttacaaat cttaaagcag ccaaaaaagg
    15661 tagtatggtg tactttaaag ttggtaatga aacacgtaag tataaaatga caagtataag
    15721 agatgttaag cctacagatg taggagttct agatgaacaa aaaggtaaag ataaacaatt
    15781 aacattaatt acttgtgatg attacaatga aaagacaggc gtttgggaaa aacgtaaaat
    15841 ctttgtagct acagaagtca aataatctat tacgctaatg gatgaatata ttgagtggaa
    15901 aacagtcttg attgcgagac tgttttttgt ttggtatgag gtagcaatga cgacgtgtca
    15961 ttggtggaga ttgtaaaaat acataataaa aagaagcggc aatgtatacc gctccttttt
    16021 tatactacat accgattttc aaccatctct ttctacttag taataagaca atagtattaa
    16081 ctataaatag aagaacgaag aatgatacta tatttataat ttcagtagga cacataaatg
    16141 ttgactcgtt attcaatatt ttttctacgg cacgatacat cgtattgctc gcctcaaatg
    16201 gagcaacgat accaaatata tttttattaa tggcaactaa gatgactgaa ccaatccaat
    16261 atacaatgct gatacctaag ctgattaaaa tgttaggtga aaccatacta atcgttccaa
    16321 caactaagat atattgtaag ataacgagtg aaaataagat tattaatagt aagtaatgtg
    16381 agaaatccga atatataatt ga
//
LOCUS       DANJFZ010000056        16361 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.056, whole genome shotgun sequence.
ACCESSION   DANJFZ010000056 DANJFZ010000000
VERSION     DANJFZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.056"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            248..1114
                     /locus_tag="PWN45_001575"
     CDS             248..1114
                     /locus_tag="PWN45_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL0623344.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            1134..1634
                     /gene="queF"
                     /locus_tag="PWN45_001576"
     CDS             1134..1634
                     /gene="queF"
                     /locus_tag="PWN45_001576"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL0623345.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            1975..3480
                     /locus_tag="PWN45_001577"
     CDS             1975..3480
                     /locus_tag="PWN45_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL0623346.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3558..3659)
                     /locus_tag="PWN45_001578"
     CDS             complement(3558..3659)
                     /locus_tag="PWN45_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623347.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            3750..4667
                     /locus_tag="PWN45_001579"
     CDS             3750..4667
                     /locus_tag="PWN45_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0623348.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(5291..5833)
                     /locus_tag="PWN45_001580"
     CDS             complement(5291..5833)
                     /locus_tag="PWN45_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDL0623349.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(6184..7242)
                     /gene="hisC"
                     /locus_tag="PWN45_001581"
     CDS             complement(6184..7242)
                     /gene="hisC"
                     /locus_tag="PWN45_001581"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL0623350.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(7482..8996)
                     /locus_tag="PWN45_001582"
     CDS             complement(7482..8996)
                     /locus_tag="PWN45_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL0623351.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(8989..9966)
                     /locus_tag="PWN45_001583"
     CDS             complement(8989..9966)
                     /locus_tag="PWN45_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623352.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(10188..11969)
                     /gene="recQ"
                     /locus_tag="PWN45_001584"
     CDS             complement(10188..11969)
                     /gene="recQ"
                     /locus_tag="PWN45_001584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL0623353.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(11981..13864)
                     /locus_tag="PWN45_001585"
     CDS             complement(11981..13864)
                     /locus_tag="PWN45_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0623354.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(14135..16075)
                     /gene="ltaS"
                     /locus_tag="PWN45_001586"
     CDS             complement(14135..16075)
                     /gene="ltaS"
                     /locus_tag="PWN45_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDL0623355.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         5176 a         2850 c         2355 g         5980 t
ORIGIN      
        1 atctcaattg tatcaacaga aatcaagaaa aagcttatta gcttgatact gaattgatat
       61 ttgttttatg taataacaca aacattcata aaagtgtaat gccctattat tccgacaaat
      121 aaacacagaa cgtttgttcg ctacagtcta ttatagtctt tttatagtaa atttcaacta
      181 tttatgcaca acatgttgtt ttatgctaat ataaatacgt taattttata taaaagaggc
      241 gataaaattg aatcctaaag taaaaggtat cattgccatt ttaatttcgg ctattggttt
      301 cagttttatg tcagtgtttt tcagacttgc aggtgacctt cctgtttttc aaaaatcatt
      361 agcacgaaac ttagtagcaa tgtttattcc attatttttt atatataaat accatcagcc
      421 aatgtttggc agactttcaa gtcaaccctt attaataaca cgatcaacac ttggacttat
      481 tggtgtattg ttaaatattt atgccattga tcatatggta cttagtgatg ccgattcatt
      541 aatgaaactc aatcctttct ggacgatttt attaagcatt gttttcttac atgaaaaggt
      601 aaggaagtat caaattacag caatgataat tgctatttta ggtatgcttt taatagtgaa
      661 acctgaattt tcatcatcta tgattccatc attagctggc ttattctcag gtatttttgc
      721 agcatcagca tatacatgtg ttcgagcact tagtacaaga gaagcaccat atactatagt
      781 attttacttc tccttatttt ctgtcatcgt tttaatacct ttcactgcat atacttatga
      841 accaatgagt cagatgcaaa tactttatct attaggggct ggcttagctg ccgctgtggg
      901 tcaaattggt gttactttag catatagttt tgcagcagca aaagatattt ctatttttac
      961 gtatgcatct ataattttca cagcaatact tggatttatt ctattcggag aatctccaga
     1021 tttctatgca acattaggtt acgtcgttat tatcggtgca agttattaca tgtttgaaaa
     1081 agcacgccga gacgccaaaa taataaaaaa ataaatttaa aggagcgctt tatatggcac
     1141 atggccgtca acaagatgaa ttacaagata taactttatt aggaaatcaa gacaatactt
     1201 acaattttga ctatcgtcct gatgtattag aatcgtttga taataagcat caaggtcgtg
     1261 actatttcgt aaaattcaac tgtccagaat ttacatcatt atgtccaatt actgggcaac
     1321 cagattttgc aactatctat atttcatata ttccaaatgt taaaatggtt gaatcaaaat
     1381 ctttgaaatt atacttattt agtttcagaa atcacggtga ttttcacgaa gattgtatga
     1441 atattattat gaatgatttg atagagctta tggacccaca ttatattgaa gtctggggca
     1501 agttcacacc acgtggtgga atttctattg atccttatac aaactatgga cgtccaaatt
     1561 ctaaatatga aaaaatggct gagcatcgtt tgatgaatca tgatttatat cccgaaaaaa
     1621 tagataatcg ttaaatgtat catttaataa acacaccaat aagttgattt tcctaactta
     1681 ttggtgtgtt tttcatttag catacataat aggttacatt aaaataacat tttataccaa
     1741 agtacaccaa aagaatatta gtacacgaat taaacaacat ttttatagaa acctattgca
     1801 ctttaacgtc aataagtata tttttatatt atctctaatt aattgtgcgc gcttaataac
     1861 agaatattct caatattttt atttttttgt gatttgttgg aatatttagt tgataaggca
     1921 caatcaaatt tacttaaact attgtattag gggaagaaag gatgggatgt atacatgaca
     1981 caacaaaact cccatggaaa tcaaattcaa gacatacctc aaacaggatt tttcgggcat
     2041 cctcgaggac taggcgtact cttctttgta gagttctggg aaaggtttag ttattatggc
     2101 atgcgtgccc tactcatttt ctacatgtac tttgccgtaa cagataatgg ccttggaatt
     2161 gataaaacaa cagctatgtc aattatgtca gtttatggtt cattaatcta tatgacatca
     2221 ataccaggcg gatggattgc tgacagaatt acaggcacta gaggcgctac tttattaggt
     2281 gcagtcttta ttattatcgg acatatttgt ttaagcttac catttgcatt aatcggctta
     2341 ttcacatcaa tgttcttcat cattattggt tcaggtttaa tgaagccaaa catttcaaat
     2401 atcgttggcc gtttatatcc tgaaaatgat agacgtatgg atgcaggttt tgttattttc
     2461 tatatgtcag ttaatatggg tgcattatta tcacctatta ttttgcaaca ctttgttaat
     2521 gttaaaaact tccacggcgg attcttgatt gcagcagttg gtatggcatt aggtttagta
     2581 tggtatgtac ttttcaaccg caaaaactta ggtagcgttg gtatgaaacc tactaaccca
     2641 ttgacaccag ctgaaaagaa aaagtatggt cttattatcg gaagtgttgt tttagcaatt
     2701 gtattaatta tcgttattgg tgcattaact aactcattat catttaactt agttagtaat
     2761 actgttttag tattaggtat tgcattacca atcatttact tcactttaat tattagaagt
     2821 aaagatgtca ctgatactga acgttctcgt gttaaagcat ttattccatt atttattctt
     2881 ggaatggtgt tctgggctat tcaagaacaa gggtctaatg tattaaacat atatggaatt
     2941 gaacattcag atatgaaatt aaacttattt ggttggaaaa caaactttgg tgaagcgatc
     3001 ttccaatcaa ttaacccatt atttatttta ttactagctc caattatttc acttttatgg
     3061 caaaagcttg gaactaaaca acctagcctg ccagtaaaat ttgcaattgg tacgttctta
     3121 gcaggtgcgt catacatact aattggtatt gtaggttatg catcaggttc atcaaacttt
     3181 tcagttaact gggttatttt atcgtacatt atttgtgtta tcggtgagct atgcttatca
     3241 ccaacgggta atagtgctgc tgttaagtta gcacctaaag catttaatgc ccaaatgatg
     3301 agtatttggt acttaactaa cgcttctgca caagcaatca atggtacttt agttaagtta
     3361 atagaaccac taggtcaaac aaattacttt attttcctag gtgttgttgc aattattgtt
     3421 acaacaattg tattagcaat ctcaccttta atcatcaaag cgatgaaagg tatacgttaa
     3481 tattgttggc ctaattcaaa aaacagtaag tcatttaaat ggcttactgt ttttttatag
     3541 gtttctatta attaaattca agatatcagt ataaataaaa acttaatatg ctcattatag
     3601 acagctataa ctatattttc tcgtcccact ctatagaaat caatttcatt tattaccatt
     3661 ttctctattt aaaaacatat ttttggtatt aacaattaaa agtgggtata tatatttaat
     3721 gaaccaattt ttagaaagta ggctaaacta tggaaaataa atatacacat ggtgtcctct
     3781 tttaccatga acacagtgga ttaaaaaata ttaatcaagg tattggggaa gttacaacag
     3841 cattaagttc aatttgtaag catctttcta ttcaattgag tgaaaacgaa ggtgacatta
     3901 ttaaatattg ccaagaaatc aaaactaaaa actatgcaaa agatgtagat atattattta
     3961 ttcttggcgg tgatggcacg gttaacgaac ttataaacgg tgttatgaca catgaccttc
     4021 aactccctat tggcatttta ccaggcggta cttttaatga ttttacaaaa acgctaaata
     4081 tagcacctaa tcataaacaa gctagtgaac aaatgatttc tgcacaagtt ggtacatacg
     4141 atgtaattaa aataaataat caatatgcac tcaactttgt tggcttaggt ctcattgttc
     4201 aaaacgctga aaacgtacaa gatggttcaa aagatatatt tggtaaattg agttatattg
     4261 gatcaactgt caaaacactg ctaaatccaa cacaatttaa ttatcaatta tcaatagatg
     4321 ataagacata ttctggcgaa acgaccatga tactaacagc aaatggtcca ttcattggtg
     4381 gtagtcgtat accattgact gatttgtctc ctcaagatgg tgaactgaac actttcattt
     4441 ttaatgaaca aagtttcagt attttaaatg atatttttaa aaagcgtgat agcatgaatt
     4501 ggaatgaaat tactcaaggt attgagcata tacctggtaa aaagatttca cttacaacag
     4561 atccagcaat gaaagttgat attgatggcg aaatttcatt agaaacacct attgatattg
     4621 aggttatacc aaatgccatt cagttactta ctgtcaatga tttgtaagca tccggaatga
     4681 tcttaacccg atattatatt aggtttggag tgtaatcata agattcaacg caccttatat
     4741 tcgtttttat tagctttaag ctattaccct gtttcaaaaa cagacatata attttggcgc
     4801 atgccactaa aatgattgat gttgtatttt aaatcatagt ggtttatgcg tctttttcaa
     4861 attctataaa aaatcggatg acgtgtaatc tgccatagat taacacattc atccgattta
     4921 taataataag ataaactaac atttattgag agcgggacgg aaatgataaa gaacgactaa
     4981 tgattgatta tgtagcgatt ctttatcatt agtcacagct aatgtgtact taaaaatatg
     5041 aatgcatgag ttacactcat gcataagaaa tactaatttc taaagaaaaa gtatttcttt
     5101 atgttggggc ccaccccaac ttgcattgtc tgtagaaatt ggcgttccaa tttctctatg
     5161 ttggggcccc gcagtcaact actgccaata tagtgttgta gtgtctaaga cataaaagtt
     5221 catctcagtc acaattactt tatagcctat ccatcatctt ttctacatga aatttttcaa
     5281 gtgatatatt ttatttctca atattatcta aaaaatactg ttctacatct ttccagctat
     5341 ttacgcgttc aaatcgatca tcattaatat tatgcactgc tgtaaacata atcggtgtac
     5401 cagtaaaaat ttcaagctgt ctaggattgt catctattaa ataatcagct ttaacgatgt
     5461 tttttctacc acaaaaaaca aaatgctgag gatctaaaaa tggaaagaac tctagtaacc
     5521 attcatattt atcactaaat gatgttggta catccattgc tgctgtagca atatatacat
     5581 cataatgttc agttaatttt ttcacaactt cttgtgcata cggcatcact ttaagatgtc
     5641 tgaagaagcc tgattctctc aatacttctg taattaatcc atcatgttca ggaataacat
     5701 gtttaagttt ttgaccattc aaagcttcca ttttaatacc taaatccgct ctaaaattga
     5761 cagcatcaat gatttctcct aatgtatctg ccaatacttc atccatatca atcgcgattg
     5821 atttacgggt cattttaatc ttctcctcaa aatatcagta ttgattcagt atagcaaaaa
     5881 agcaattgat acaaaacaat atcatctatt gttgcttttt acactgaata ctctcacctc
     5941 atcattcaca ctgcattgag cttgagacat aaatcaatgt tcaatgctct acaaagttat
     6001 attggcagta gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca attctagtca
     6061 accttgccgg cggggcccca acaaagagaa attggattcc caatttctac agacaatgca
     6121 agttggggtg ggacgacgaa ataaattttg cgaaagtatc atttctgtcc cactccccac
     6181 ttactattcg tatttaaagt ttgataaaac ttctaacatt ttatcatttt gttctttaaa
     6241 accaattgta attctaacac cagttggaaa tggtcgcgta atacacccta catttaaaag
     6301 tgcttcataa agttcattta cccgctttgt ttttacaaat ataaaattcg tttgacttgg
     6361 caagaaatac tcactttgag gtaattgata aaatctttcg cgttcaacac tatttttatg
     6421 tgttacctct tttaaatatt gttgatcttc aagtgctgca actgctgcgt attcagatat
     6481 acgtgtcaca ttaaatggtg gtctaatgat gttccatttt tcaatcgcat gttcacttgc
     6541 taccacatat cctacacgta aacccgctaa tccatacgcc tttgaaaatg tacgtaataa
     6601 gaaagcattg tcatattttt gttgcaaagc aagtgtatcc gggtagtcct ctgctgtcac
     6661 aaattcaaaa taagcttcat ctataattac tggtacatgt ggaggtactt gagataaaaa
     6721 cgaatctaag ctctcatgat taaaatatgt acctgtagga ttatttggat tacataacca
     6781 taccaatgac gtatcttcat taacttcttt taaaatacct tctaaatcga agccaccatc
     6841 ttttaaaggt acttgtatca cattagctga ttcaacaatc gcattgtgat aatattgacc
     6901 gaatgtcgct tcacttgtaa caatagtatc ccctggcgtt aatacagctc tagaaatcat
     6961 taatataact tcatctaatc ccgcaccaaa taaaatgcgt gattgatcta catttaaatg
     7021 tttactaatc gccgctttta atgtcggtga tcctgtttca ggataataat ataactcatc
     7081 taagtgtgct gatatcgctt ctttaacttt aggcgatggt ccatacaaat tttcatttga
     7141 tgcaagttta tataaatctc cttcaatgcc atacttttct ttcaatgccc ttggagataa
     7201 accaggctga tatgctgata gttgattaag ttgttctttc ataataaaaa caacctcctt
     7261 agaattaact gaaatttcaa aaaacttata ttcattatat cgctctatgt ttctaaaatg
     7321 caatgctact attttcacat cataatagct tacattgtgt ttaacatgtc atatttttta
     7381 atcattattg catgtttaaa ttgaacataa ttatagattt aaatataaca acgccacata
     7441 agatataatt tggtgttacg catcttatgt ggcgttggtc gttatttgat taaccctttt
     7501 gcttttaaat aatctttcgc aactgtatat gggtcttcat ttttaactgt tactttatag
     7561 ttcatcattt gcatatcttc atcagatatt ttgttttcta gtttgtttaa cggtttctta
     7621 atttctggat gtttctttaa aaagctttct ttaaataatg gtgctccttg atatggtgga
     7681 aatacgtgct tatcatcttt taacacaacc atatcatatt gttttaattc agcgtcagtt
     7741 gaatatgcat ctattaaatt aatattacct ttattgatcg cttgatatct caacttaggt
     7801 tccattgtac gtatgttatc taaatttaaa ttataagcct tttgaacagc tttgtaacca
     7861 tctggacgat cattaaattc caatgtaaat cctggtttaa gttgatcttt aaccttattt
     7921 aaatcaccaa ttgtacgtat attatgttgt ttagcaaaat cacgttttac agctaaagca
     7981 tacgtattgt tatacttcat tggttttaac atagtcattt gatatttctt ttcaagactt
     8041 tgcttagctt gttcataaac ttttttctct tcttttgact tcaatggttc ttttgttaat
     8101 tcacctaaaa ctgttccagt aaattctaaa tacccatcta tatcgtcaga ttttaaagca
     8161 ttaaataaaa atgctgtttt gcccatacca tctttcactt ctacagtatt tttggtctct
     8221 tcttctatta aaattttata catatttgta ataatcgatg gctcggagcc aagctttcca
     8281 gctaacgtaa ttttatcacc tttttgtgca aacataggaa tagcgatagc cagtataata
     8341 atcatcacta taacacctaa cgtcatcaat aactttttat aagataattt agccataaat
     8401 cttaaaatta aatcaaatat aattgccaat aaggctgctg gaattgcacc taataatatc
     8461 aacgatgcat tgttacggtc tatacctaat aaaattaaat ctcctagtcc gcctgcacca
     8521 attaatgctg ctagtgttgc tgtacctata attaatacca tagccgttct tacaccagcc
     8581 attataacag gcattgctat cggaagttcg actttagtta aacgtctaaa tggtttcata
     8641 cctatacctt tagccgcttc aatgagtgat ggatcaactt ctttaattcc agtatacgta
     8701 ttccttaaaa ttggtaacaa cgcatacact acaagtgcaa taattgctgg cacacgaccg
     8761 ataccaaata aaggaatcat taaacctaat aatgccaacg atggtatggt ttgaagaatt
     8821 gccgcaatat tcattacgat ttcagatatc gttttagtct tcgttaataa aatacctaat
     8881 ggtaccgcaa tagcagttgc aatcaataat gcgataaatg atatttgaat atgttctatc
     8941 attgtcgaaa agagttgccc cttacgttca ctcaatatgt cgaaaaagtt agtcatgttg
     9001 agctacctcc tttttctggg acaaatattt gaagatatct ttcctatcaa taacatattg
     9061 acctacgcta tcttcttgca tgacaatgac acgctcgctc tctgataaaa gttgatacaa
     9121 tacttcaatt ggttgattgt cataaacaat tggataagcg ctcatagatg taacctcatc
     9181 gattggtttc ataatatcca agtcacggat aattgcgttc tcttcaacac atggcgcatc
     9241 atcttctaaa tgactaccca taaattgttt aacaaattca ctttgaggat tatttttaaa
     9301 tccttctggt gtgtcaattt gttcaatatg cccttcattc aaaagacaaa tcttatcacc
     9361 aagtttcatc gcctcttgaa tatcatgtgt aacaaatatg attgtcttct taattttagt
     9421 ttgtaattca attaaatcat cttgaagttt ttctcggctg attgggtcta atgcactaaa
     9481 cggttcatcc attaaaataa ctggtggatc agctgctaac gcacgtataa ctcctacacg
     9541 ttgtcgttgc ccccctgaca attcatcagg ttttctgttt ttatattttt caggttctaa
     9601 tccaaccatt tcaagtaatt catctactct tttatctata tctttttctt tccacttttt
     9661 catttgtggc acttgtgcaa tattttcttt gattgtcata tgtgggaata atgcaatctg
     9721 ctgcaatacg tatccaatat cccaacgcat ttcgtatact ggataatcac ttattggttt
     9781 atctttaaaa taaatataac cttcacttaa gtgaatgagt cgattaatca tttttaatgt
     9841 cgtagttttt ccacaacctg aaggtccaat tagcacaaaa aattcaccct cattaatatt
     9901 gaaactaatg ttatcgacag caacatgttt gccataacgc ttagttacat ttttaaactt
     9961 aatcactttg ccacctcttt ttttctcata gcataaaacc gagattatat gtatgtattc
    10021 cctatttaac cacgtttatt acaattttca aatttaaatg atttatcctt gaactttttt
    10081 aataaaataa tgaataatag gtaatctcca gttaagaaat agtgttattt taccttgaat
    10141 tcaaaaaaac acccagtaaa acaaggaatg cttactaggt gtcttcacta tactttggct
    10201 ttataatttt gaatcgtttc taaaaatgct ggacaataat gttttaattt gtaactacct
    10261 acgccatcaa tattaatcat atcttgtttc gaagcaggct tacgtttagc aaattcctcc
    10321 aacgtgtaat cagaaaatat acttacaggt gctatcgtta atttgtcact taacttttta
    10381 cgaacttcta ccaactgact gaataatact cggtcaaccc cttcaaccgt atttataaat
    10441 actttttcag tcgctttttg cttaaatggt gttgtgaata cttctacttc attactgagt
    10501 aattttttaa ttgaagtatc acacattaat atttcgtcat tttcatttaa gaaccctttg
    10561 aatcttaatt catctattaa gtgacttaat tctgatgttg tgtaaccttt cattaaacca
    10621 tgggttgaaa tttggtcata acctttatac ttaatataat ctgttgactc tcctcttaac
    10681 acttgaatga taacactata actctcttgt tgtttcatac gagcgatgca actaataatc
    10741 atcttagctt cttgtgtcat attatatgat ttatcttgtt gaacacaatt actacattgt
    10801 tcacattctt ctaatttttc attcggttca aaataatgga caattgttgc ttctagacat
    10861 ttttttgttt ttgtatattg aatcatttta gttaactttt cgcccatttt atctttatag
    10921 tcatcatcag cttgagagac tgttataaaa tactcgtgta aattgatatc gcgttcgcta
    10981 aataacaaaa tacattcact tttcaacccg tcacgacctg cacgacccgc ttcttgataa
    11041 taagattcta aatctccagg catattataa tgaataacaa agcgtacatt ggatttatca
    11101 atacccatac caaaagcatt tgtagcaacg actactttaa cacgatcaaa taagaaatca
    11161 ttctgcgctt cttctctttc tttattgctc aaacctgcat gatatataac actttcaatt
    11221 ttctgacttt ctaaggcttc ttgaagctct tcaacttgct tacgtgtaga acaataaata
    11281 atacctgcat cttcatcgtg tgttttaata taatccaata taaatttttg acgttgataa
    11341 gtaggattta ctttaaaaat taagtttcta cgcttagtac tcgttttaat ttgatcagtt
    11401 tgagcgatat ttaacttttc tctaatatct tgctgtactt caaccgtggc agttgctgtc
    11461 aacgctatta ttgtaaaatc ttgaggtaac gtaaatactt ttgaaataac attttggtaa
    11521 ctcggcctga aatcatgacc ccatttagaa atacaatgcg cttcatcaaa cgcgactaag
    11581 tgaatcttta tacgctgaag catatttaaa aaatatcggt tttcaaatcg ttctggtgca
    11641 acatacaaaa attgaatttc tccatttgat aatgcttttt caatacgttg ttgctctttt
    11701 tgagtcaaac tactatttaa aaaagcagct tgaattccca tcgcttttaa ttgatccact
    11761 tgatctttca ttaatgatat tagtggactt attacaattg ttgtaccacc taacaataaa
    11821 cctggtactt gatagcatat agacttacct ccaccagttg gtaagacacc aagcacatta
    11881 cgatggtcta atactttgct aataatttct tcttgtcctg gtcgaaacgt ttcataccca
    11941 aagtaatgcg ataatgtttg ttgcatcata aattgacccc ttattgttgt tctttaattt
    12001 cttctaactc actccatctt gtgatgtcta aatcatattg aatttcaagt tgttcttttt
    12061 cttcgtttaa ttctttaatt ttcccataat ctgcacttgc ctcaatcatg agcacatcaa
    12121 tttcttccat tcttacttcc gcttgttcta tgcgtttcat caattgttca tattctaatt
    12181 tttctttata tgataaacca tttttcttac gtacagttgt agaagattta gattgttgct
    12241 tcaatgtgga tttattttta tctaatgatt ttttataact ttcataatct tcaaaagttc
    12301 cgataatctt ttccatctga ccatcatgaa taaaccaata tgactgtgca actttattta
    12361 agaagtagcg atcatggctt acggtaatca ctgtaccacc gaaagtatga atataatctt
    12421 caagtattgt taaagtctca gtatctaaat catttgtcgg ttcatctaac aacagaacat
    12481 ttggctggtg tacgagtaga cgtaataaat acaaacgctt ttgctctcca ccagataatt
    12541 tataaacttt tttaccatga gttgcacttg gaaataaaaa tcgttcaaga agttgtgtaa
    12601 tagataccga ggttccatct ttttcttttg cgatctcatt ttcttctctt aaataatcaa
    12661 tcatacgaat atctctatcc agggtctcat ctgtttgctt aaaataagct actttaaccg
    12721 tctgcccagt cttcaattta ccttcgaatt gttggtcttc tccactcaaa atatttaaga
    12781 gtgttgtttt accagctcca tttggcccaa caacaccaat acgctcacct ttttgaataa
    12841 tttccgtcag atgttcaatt aatactttat cattaatagc ctttgataag tcttctaatt
    12901 cgaacacttg cttacctaat cttgaataag caagattcaa ttcaccttta tcgtctttat
    12961 attgctggtt aacttcattt tctaggtcat taaatcgatt aattctagct tgttgctttg
    13021 tagtacgagc cttagctcca gccctcatcc aagcaagttc ttccttatat aaagctcgtt
    13081 gcttttgttg ttgcttttga agtgttactt ctctttcagc gcgcatttca atataagatt
    13141 catagttacc aggatatgac gctaacttac ctctgtttag ttcaataatt ctagtggaaa
    13201 cttcatttaa aaaatatcga tcatgggtta cgaataaaac agtatgagga tattgcttca
    13261 cataattgat caaccagctg attgattcga agtctaaatg gttcgtaggt tcatctaata
    13321 acaataaatc tggttgttct attaatgttt tagcaagtac aacacgtttt tgttgtccgc
    13381 cggataattc tttaatgtat ttagtagtat catgtatacc tagttttgag aggattgttt
    13441 taatttcagc gttatagtcc caagcatcat gttgatccat agcatcttgc gcatccatca
    13501 ttcgcttgaa caatttatca ctttggtcat ccgcataagc ttgtactgcc tgctcatatt
    13561 gcttgatgat gcgtaaagtt gttgtatcag aacttaatac tgcatcaaaa actgtcatat
    13621 cttcattaag gtcctgtttc tgagacgaat atcgaattcg atattgattt ggatgcataa
    13681 cattggctgt aaaatcatca tcaataccac caattacttt taacaacgta cttttccctg
    13741 taccatttat gcctactaaa cctatttttt caccttctga aattgataaa tctaggttat
    13801 cgaatatagt cttatcggca taagatttat ttaaatgttc aattttatat gcttccatac
    13861 ccatcttcct tcttctgttg aaatctctac gtacttaaat cagttctata cattatacac
    13921 ctttttgaaa tcaaaagaaa tcgataccgt taagtgtcat aatttaatat attgttgctt
    13981 caactataaa taaaacttgt taaggctttg atgactatgc taatgatctt tataatatac
    14041 ctttttatca acacatataa tgtaaaacat aatataaaag aacataataa aaacccggat
    14101 tgatatttta tcaatccgag ttcgtgttta gatattattt tttagagttt gctttaggtc
    14161 ctgtttcata tttatacttc gaaggattta cctttttgaa gtctggattt ttgtagaatc
    14221 taaacaaatc accattaagc acgttgtcac tcatttcgag atccttttca acttgctttt
    14281 tattcttttc gaaatcagct ggttgagtag ttatgagttc attattttta ttagaataaa
    14341 tcttaccatt aacatattta taatcttttg ttataaagtc accatttctg aatggaacta
    14401 cttgattatg acctttagag aataaatcag taccgaacat taaatagttc ttcgtatcta
    14461 taccagccaa atgtaaaatt gttggcatta catcgacttg accagcatat tcattattga
    14521 taccaccaga tttaccaggg attttaatcc agaaaccagt tctgtttaaa tctgtaaatt
    14581 tagccggtgt gattttttca cctaatagtt tttccatggc attgttatgg ttttcagaga
    14641 taccatagtg gtcaccataa atcataatca ctgaattgtc atataatcct tttttcttca
    14701 agtcattaat atattcttct aatgcttcgt ctaaataacg tgctgtttga atataaccat
    14761 caactgttgc atcacctgtg tttgatttct caatagttgc atccttttca tctaatgtga
    14821 atggatagtg gttagtcaat gtaattaaat gagaatagaa tggtgatttc atcttagctt
    14881 gataattagc agaatcttta aagaaaattt tgtctttcaa gcctaagttt acaacgtttt
    14941 tatctgacat gtcatagtat gttgcatcat agaatttatc gataccaaag tgtttatata
    15001 cttggtctct gttccagaat gttttatagt caccgtgcat gacatcagat ttgtagcctt
    15061 gcttttgatc taaaattgct ggtaatgact gatacgtatt atctcctttt aatgaaaagg
    15121 cagaaccttg cggtaaaccg tataaactgt tatccattgt aaattcagag tcagatgttt
    15181 taccttgacc tgtttgatgg aaaaagttag ggaagtatgt gaattgctct ttcccacttg
    15241 ataatttgtt taaaaacggt gttacttctt taccattaac ctttttatta attaagaagg
    15301 tttggaaact ttctaaatga atcttaataa tatttttctt ctttgccacc ccataatatt
    15361 ctgggttagg ctctgtttga cgttgtttcg tataatttaa tacttttgtt aagtcatctt
    15421 cagatgctag cgctttttgt tgattatttt cgatagtttt aacaccatcg tatactgtaa
    15481 agttataagg tcctaaatat ttcactaaat atttatggtc aaatgtacgt gttaataatt
    15541 ctggtctgtc agtttcagca aaagctaagt ttaagaagaa taatgctact gaagccgcca
    15601 ttacgacagg aacaaatttc ttactaaatg cttttgtgtc taaccatttt gttttaaata
    15661 ttaaaatgaa taagtaaact aacgtatcaa tgaaataaac aaagtcatac catttgaatg
    15721 atgcactaac cgcaccaccc atagattcta cgttacctac ttggtttaaa gtactaaacg
    15781 ttaaaaaatc agagaagaat ctaaagtaca caacattggc atataatagg aacgtcaata
    15841 agaagccgcc tatgaacatg aaccaaaatg cttttttgcc tttaaagaat aggaacacac
    15901 ttaaaaccag tgctactaaa ctataaggat tcatcaataa tattaagttt tgtactaaac
    15961 ctttaacacc taaagaaaaa tcaacataat aagaaaaata cgtcttcaag gtaatcgtta
    16021 ttacggttaa taagaagaac gcaaaaagac taattttctt tttttgtgaa ctcatgattc
    16081 tttcccccgt tatttagata ataaatcttg cgaaagtact tgaataaatt agatattaac
    16141 attatgtagt taattaataa gtgcgtttaa aagttaacat tgtcataatt atagtaatta
    16201 ctttcagatt caagtttata attttcgtta tttgtagctt tcaactttga tttaacaagt
    16261 attttgggct acaactcata tttgcttgtg taagtttctt tatttaatat tatcattcaa
    16321 ttaacaaatt taaaatttgt aatcaaattg taataataag c
//
LOCUS       DANJFZ010000057        16228 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.057, whole genome shotgun sequence.
ACCESSION   DANJFZ010000057 DANJFZ010000000
VERSION     DANJFZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.057"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            139..792
                     /locus_tag="PWN45_001587"
     CDS             139..792
                     /locus_tag="PWN45_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDL0623356.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            826..1413
                     /locus_tag="PWN45_001588"
     CDS             826..1413
                     /locus_tag="PWN45_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDL0623357.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1556..2449)
                     /locus_tag="PWN45_001589"
     CDS             complement(1556..2449)
                     /locus_tag="PWN45_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0623358.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2697..2990
                     /gene="esxA"
                     /locus_tag="PWN45_001590"
     CDS             2697..2990
                     /gene="esxA"
                     /locus_tag="PWN45_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDL0623359.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3074..6103
                     /gene="esaA"
                     /locus_tag="PWN45_001591"
     CDS             3074..6103
                     /gene="esaA"
                     /locus_tag="PWN45_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDL0623360.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6103..6561
                     /gene="essA"
                     /locus_tag="PWN45_001592"
     CDS             6103..6561
                     /gene="essA"
                     /locus_tag="PWN45_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDL0623361.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6533..6775
                     /gene="esaB"
                     /locus_tag="PWN45_001593"
     CDS             6533..6775
                     /gene="esaB"
                     /locus_tag="PWN45_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDL0623362.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6788..8122
                     /gene="essB"
                     /locus_tag="PWN45_001594"
     CDS             6788..8122
                     /gene="essB"
                     /locus_tag="PWN45_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDL0623363.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8144..12583
                     /gene="essC"
                     /locus_tag="PWN45_001595"
     CDS             8144..12583
                     /gene="essC"
                     /locus_tag="PWN45_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDL0623364.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12613..13005
                     /gene="esaC"
                     /locus_tag="PWN45_001596"
     CDS             12613..13005
                     /gene="esaC"
                     /locus_tag="PWN45_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDL0623365.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            13021..13335
                     /gene="esxB"
                     /locus_tag="PWN45_001597"
     CDS             13021..13335
                     /gene="esxB"
                     /locus_tag="PWN45_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDL0623366.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13335..14009
                     /locus_tag="PWN45_001598"
     CDS             13335..14009
                     /locus_tag="PWN45_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDL0623367.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            14009..14326
                     /locus_tag="PWN45_001599"
     CDS             14009..14326
                     /locus_tag="PWN45_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623368.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14336..16180
                     /locus_tag="PWN45_001600"
     CDS             14336..16180
                     /locus_tag="PWN45_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDL0623369.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
BASE COUNT         6118 a         2220 c         3056 g         4834 t
ORIGIN      
        1 ataaactaca aaaaatagtt tgattacata atgattcttg aaaaatgttg gttaacttaa
       61 taatatgcat ttttttggcg aagaagattt atttaactta taaaaatatt gaagtaagat
      121 tggggagatt atgaatttat ggaattgaaa gtcgatgatt ttgtaaagaa tataaaaaga
      181 ccatacttga ctgtattggg agtatttgta gttgcagttt cattattttt tgacgcgatt
      241 atgtttttct acgctaaact atatgataaa ttgcctatgt atttactggt gtttatggcc
      301 tttacagctg taattttgat tatgatgtac atacaagaga aaaatgaaaa ttacaaagtt
      361 gaaaaaagat atgtggttag atatctcaca cttaacgtta ttgtgggata tactttgcca
      421 ttgctttttg tatctattta cgtttttggt gtagtcggtt ttggatttga tgttttcaat
      481 tattgtctag gtattatctt gatgttattt atttcttggt taggtttatt tttattttat
      541 aaaaacgaat ttgatagtga aaatcctaat cctgcagtta atgccatagc aattattata
      601 aaattatttg cttttggagg aatattttat ataagtctaa tagttcctgt aacacagcaa
      661 gaagagattt ttatagggtt aagtatattc atcaatataa ttgttgatgc tcttcttgtt
      721 aggtcatatt ttaattacgc gttatataag agtattaaga aagatatcga aaatgaaggt
      781 aagacaatgt aaaggaaaga cataaaaaat aataggagtc taaccatgat aatttttatt
      841 ttaataacaa tatttgctat ttattatata gctatgattg ctagtttgtt taaaagtgaa
      901 ggtttttcaa taataggttt aatattagat gttgttataa tggggacatt gattttttac
      961 tattttatag gcgctcgctt tgttgatcat gatttaagca actttttaat gtttatggat
     1021 actggatcat atatttttat gtactttgca attaagtgtt tatgggtgaa acctaaggta
     1081 gtaaattatt tgattgcaaa agaattgggt gaatctaaag aggtcattga agagcaggaa
     1141 ttagatttac agacatcaaa gataagaggt atctactttt tcattatttc agtagtgatg
     1201 ttaattatca ctaaattaag aatgcaacct gagttacagg cagatgctat atcgatgaat
     1261 cctgtattta tttttgttgg tgttataatc attttaattt ggctagtact ggatatatat
     1321 cgcaagaaaa aatacggtat attcttattc aagacgatag tgccactagt tgttacgact
     1381 tggattatta tagctacaat tatactttcg taaacgattt tgattaatgg ggaatgtaga
     1441 gaacatttct ttgtaatttt ataatcgaat aacatacaaa ataaagcgac caattttgca
     1501 gtgttacgaa ttgtaaactg cgaactggtc gctttttatt gatgtcatga ttaatttaat
     1561 ggatgtaatt atatgatgaa acttctgaag cagagatggt tcttgatgaa acgatatatt
     1621 caccaatcca gttcatttct gaaattagaa tacttccatc aatattaact ttttcaacgt
     1681 aggctacatg accaaatgga ccatttactg tttgtaaaat tgatcctcgt gttgggtgtc
     1741 tatctacttt gaagccattg cttgaagctt ggcctgccca gtttttagca tctccccaaa
     1801 atgtactaat cgtgtgtcca tctttggcac gtttatcaaa gacataccat gtacattgtc
     1861 cagcagtata taagttgttc ttacttgtga taagaggctg atcaatgatt ttaccgttac
     1921 ctaatgctaa aggtttaccg tcagcggtct ttgctttgtc attaaattcg gcgatttgta
     1981 attcgtcata caattcgtct aaatcgatgc ctgtaataag ccctttgtta tcttttgaaa
     2041 aagcgttatt taattcatcg tcattgtctt cgacattcgg tattgctggt gtcaaaggat
     2101 tgcttggtga cgtttgaggc ggtgtgtgtg aatcaattgc gtcattaatg tgcgtatact
     2161 gaccacttaa tgaagaatgg tactgattgt tgttaagatc acgttgattt gcgtgttgat
     2221 gattgtcgtt tgtacgtgac tggttttgat gattgttgtt tggcgtgttg tttttgtcat
     2281 atgtataagt atacgcgccg gtgtctttat tcactttgaa ctgtgcgttt gggtgtgctt
     2341 tctttgcttc ttctaatgtt ttgctatcat tcgtatatgc ttgagccgag ttaggcgaca
     2401 tactaaataa agtaagagtt gtcatcgtca gtaaaattgt tttcttcata ataaccattt
     2461 aatcctttat gtatttaatt taattttagt atacacattt atattacaaa aatgaatggt
     2521 taattaaaaa tatatgggta tattcaatat atttatttaa aaaaagctaa aaatacttaa
     2581 aaaactatat acatatacta ataatttata tattatttga gtaaggagca ctttttcaaa
     2641 aaatagtgtc cctaaaaagt tttgataaac ttaaaatatt caggaggttt ctagttatgg
     2701 caatgattaa gatgagtcca gaggaaatca gagcaaaatc gcaatcttac gggcaaggtt
     2761 cagaccaaat ccgtcaaatt ttatctgatt taacacgtgc acaaggtgaa attgcagcga
     2821 actgggaagg tcaagctttc agccgtttcg aagagcaatt ccaacaactt agtcctaaag
     2881 tagaaaaatt tgcacaatta ttagaagaaa ttaaacaaca attgaatagc actgctgatg
     2941 ccgttcaaga acaagaccaa caactttcta ataatttcgg tttgcaataa gcattctgaa
     3001 attggcaaag tcacattttc taatgtggct ttgcttatca tttttttaag aaaacaactg
     3061 aaaggaaata agcatgaaaa agaaaaattg gatttatgca ttaattgtca ctttaattat
     3121 tataattgcc atagttagta tgatattttt tgttcaaaca aaatatggag atcaatcaga
     3181 aaaaggatcc caaagtgtaa gtaataaaaa taataaaata catatcgcaa ttgttaacga
     3241 ggatcaacca acgacatata acggtaaaaa agttgagctg ggtcaagcat ttattaaaag
     3301 gttagcaaat gagaaaaact ataaatttga aacagtaaca agaaacgttg ctgagtctgg
     3361 tttgaaaaat ggtggatacc aagtcatgat tgttatccca gaaaactttt caaaattggc
     3421 aatgcaatta gacgctaaaa caccatcgaa aatatcgcta cagtataaaa cagctgtagg
     3481 acaaaaagaa gaagtagcta aaaacacaga aaaagttgta agtaatgtac ttaacgactt
     3541 taacaaaaac ttagtcgaaa tttatttaac aagcatcatt gataatttac ataatgcaca
     3601 aaaaaatgtt ggcgctatta tgacgcgtga acatggtgtg aatagtaaat tctcgaatta
     3661 cttattaaat ccaattaacg acttcccgga attatttaca gatacgcttg taaattcaat
     3721 ttctgcaaac aaagacatta caaaatggtt ccaaacatac aataaatcat tattgagtgc
     3781 gaattcagat acgttcagag tgaacacaga ttataatgtt tcgactttaa ttgaaaaaca
     3841 aaattcatta tttgacgagc acaatacagc gatggataaa atgttacaag attataaatc
     3901 gcaaaaagat agcgtggaac ttgataacta tatcaatgca ttaaaacaga tggacagcca
     3961 aattgatcaa caatcaagta tgcaagatac aggtaaagaa gaatataaac aaactgttaa
     4021 agaaaactta gataaattaa gagaaatcat tcaatcacaa gagtcaccat tttcaaaagg
     4081 tatgattgaa gactatcgta agcaattaac agaatcactg caagatgagc ttgcaaataa
     4141 caaagactta caagatgcgc taaatagcat taaaatgaac aatgctcaat tcgctgaaaa
     4201 cttagagaaa caacttcatg atgatattgt caaagaacct gatacagata caacatttat
     4261 ctataacatg tctaaacaag actttatagc tgcaggttta aatgaggatg aagctaataa
     4321 atacgaagca attgtcaaag aagcaaaacg ttataaaaat gaatataatt tgaaaaaacc
     4381 gttagcagaa cacattaatt taacagatta cgataaccaa gttgcgcaag acacaagtag
     4441 tttgattaat gatggtgtca aagtgcaacg tactgaaacg attaaaagta atgatattaa
     4501 tcaattaact gttgcaacag atcctcattt taattttgaa ggcgacatta aaattaatgg
     4561 taaaaaatat gacattaagg atcaaagtgt tcaactcgat acatctaaca aggaatataa
     4621 agttgaagtc aatggcgttg ctaaattgaa aaaggatgct gagaaagatt tcttaaaaga
     4681 taaaacaatg catttacaat tgttatttgg acaagcaaat cgtcaagatg aaccaaatga
     4741 taagaaagca acgagtgttg tggatgtaac attgaatcat aaccttgatg gtcgcttatc
     4801 gaaagatgca ttaagccagc aattgagtgc attatctagg tttgatgcgc attataaaat
     4861 gtacacagat acaaaaggca gagaagataa accattcgac aacaaacgtt taattgatat
     4921 gatggttgac caagttatca atgacatgga aagtttcaaa gacgataaag tagctgtgtt
     4981 acatcaaatt gattcaatgg aagaaaactc agacaaactg attgatgaca ttttaaataa
     5041 caaaaagaat acaacaaaaa ataaagaaga tatttccaag ctgattgatc agttagaaaa
     5101 cgttaaaaag acttttgctg aagagccaca agaaccaaaa attgataaag gcaaaaatga
     5161 tgaatttaat acgatgtctt caaatttaga taaagaaatt agtagaattt ctgaaaagag
     5221 tacgcaattg ctatcagata cacaagaatc aaaatcaatt gcagattctg ttagtggcca
     5281 attaaatcaa gtcgacaata atgtgaataa gctacatgcg acaggtcgag cattaggcgt
     5341 aagagctaac gatttgaatc gtcaaatggc taaaaacgat aaagataatg agttgttcgc
     5401 taaagaattt aaaaaagtat tacaaaattc taaagatggc gacagacaaa accaagcatt
     5461 aaaagcattt atgagtaatc cggttcaaaa gaaaaactta gaaaatgttt tagctaataa
     5521 tggtaataca gacgtgattt caccgacatt attcgtatta ttgatgtatt tactatcaat
     5581 gattacagca tatattttct atagttatga acgtgccaaa ggacaaatga atttcattaa
     5641 agatgattat agtagtaaaa accatctttg gaataatgtc attacgtcag gtgttattgg
     5701 tacaactggt ttggtagaag ggttaattgt cggtttaatt gcaatgaata agttccatgt
     5761 attagctggc tatagagcga aattcatctt aatggtgatt ttaactatga tggtcttcgt
     5821 acttattaat acgtatttac taagacaggt aaaatctatc ggtatgttct taatgattgc
     5881 tgcattgggt ctatactttg tagctatgaa taatttgaaa gcagctggac aaggtgtgac
     5941 taataaaatt tcaccattgt cttatatcga taacatgttc ttcaattatt taaatgcaga
     6001 gcatcctata ggcttggtgc tagtaatatt aacagtactt gtgattattg gctttgtact
     6061 gaacatgttt ataaaacact ttaagaaaga gagattaatc taatgttgat gaatagcgtg
     6121 attgctttaa cttttttaac agcatctagc aataatggcg gacttaatat tgatgtgcaa
     6181 caagaagagg aaaagcgaat caataatgat ttaaatcaat atgatacaac gctatttaat
     6241 aaagacagca aagcggttaa tgatgcgatt gctaagcaga aaaaagaacg acaacaacaa
     6301 ataaaaaatg atatgtttca aaatcaagcg agtcactcga ctcgcttgaa tgaaactaaa
     6361 aaagtgttat tttccaaatc taacttagaa aagacttcgg agagtgataa aagcccctat
     6421 attcaaaaca agcaggagaa aaaaatattc ccgtacattt tgatgtctgt aggggctttt
     6481 ttgactttag gatttgtcat tttttcaatt cataaaggga gacgaacgaa aaatgaatca
     6541 gcacgtaaaa gtaacatttg attttactaa ttataattac ggcacatatg acttagcagt
     6601 accagcatat ttaccgataa aaaacttaat agctttagta ttggatagtt tggacatttc
     6661 aatatttgat gtcaatacac aaattaaagt gatgacgaaa ggtcaattac ttgttgaaaa
     6721 tgatcgactc attgattatc aaatcgctga tggagatatt ttgaagttac tataggagga
     6781 aaaatagatg gttaaaaatc ataaccctaa aaatgaaatg caagatatgt taacgccttt
     6841 agatgctgaa gaagcagcta aaacaaaatt acgcttagat atgagagaga ttcctaagtc
     6901 ttcaattaaa ccagaacatt ttcatttaat gtacttatta gaacaacatt ctccatattt
     6961 tatagatgct gaattaactg aactacgtga cagtttccaa atacattatg acattaatga
     7021 caatcataca ccttttgata atattaaatc atttactaaa aatgaaaaat tacgttactt
     7081 actcaatatc aaaaatttag aagaagtaaa tcgtacacgc tacacatttg tgttggcacc
     7141 agatgaatta tttttcacaa gagatggatt acccattgct aaaacaagag ggttacaaaa
     7201 tgttgttgat ccattacctg tgtcagaagc tgaattttta acaagatata aagcgctggt
     7261 tatctgtgca ttcaatgaga aacaatcatt tgatgcttta gttgaaggaa acttagaact
     7321 acataaagga acgccatttg aaactaaagt tattgaagcg gcaacgttag atttactaac
     7381 ggcattttta gatgaacagt atcagaaaca agaacaagat tatagtcaaa attatgcata
     7441 tgtacgcaaa gtaggacata ccgttttcaa atgggttgct atcggtatga caacgttaag
     7501 tgttttatta attgcattct tagccttttt atatttttca gtaatgaagc ataatgagcg
     7561 cattgaaaaa ggataccaag catttgtaaa ggatgattat acgcaagtac taaatacgta
     7621 tgatgattta gatggtaaaa aattagataa agaggcactt tacatttatg ccaaaagtta
     7681 tatccaaaca aataaacaag gtttagaaaa agataagaaa gaaaatttac ttaataatgt
     7741 gacaccaaat tcaaacaaag actacttatt atattggatg gaattaggac aaggacatct
     7801 tgatgaagcg attaatattg ccacttattt agatgataac gatattacaa agttagcgtt
     7861 gattaataaa ttaaatgaga ttaaaaataa cggagattta tcgaatgata aacgttctga
     7921 agaaacgaaa aagtataacg ataaattgca agatatttta gacaaagaaa aacaagttaa
     7981 agatgaaaaa gcgaaatctg aagaagagaa agcaaaagcg aaagatgaga aattaaagca
     8041 acaagaagag aacgaaaaga aacaaaaaga acaagcacaa aaagataaag aaaaacgcca
     8101 agaagctgaa agaaaaaaat agtataggac tgaggcaaag acaatgcata aattgattat
     8161 aaaatataac aaacaattga agatgctcaa tttgcgagat ggtaagacat atactattag
     8221 cgaagacgag cgtgcagata ttacgttgaa atcgttaggc gaagtcattc atttagaaca
     8281 aaataatcaa ggtacttggc aagcgaatca tacttctatt aataaggtgc ttgttagaaa
     8341 aggtgacctt gatgacatta cattacagct ttatacagaa gctgattatg catcatttgc
     8401 gtatccttca attcaagata cgatgacaat tggaccaaat gcgtatgatg atatggttat
     8461 tcaaagcttg atgaatgcca tcattattaa agattttcaa tcaatacaag aatcacaata
     8521 cgtacgcatt gtgcacgata aaaatacaga tgtgtatatt aactatgaac tacaagagca
     8581 actaacgaac aaagcttaca ttggtgatca tatttatgtt gaagggatat ggctcgaagt
     8641 acaagctgat ggtttaaatg tattgagtca gaatacagtg gcatcgtcat taattcgctt
     8701 aacacaagag atgccacatg cacaggcaga tgattacaat acgtaccatc gttcgccaag
     8761 gattattcac cgtgaaccga cggatgatat taagattgaa agaccgccac agccaataca
     8821 gaagaacaat acagtgatat ggcgttccat tataccgcca ttagtaatga ttgctttaac
     8881 tgttgtcatc tttttagtga gaccaattgg tatttatatt ttaatgatga ttggtatgag
     8941 tacagtaacg atagtatttg gtattacaac gtatttctct gaaaagaaaa agtataacaa
     9001 agatgttgaa aaacgagaga aagattacaa agcttatttg gataataaat ctaaagaaat
     9061 taacaaagcg attaaagcac aacgttttag tttgaattac cattatccaa cggttgctga
     9121 aattaaagat atcgttgaaa cgaaagcacc aagaatatat gaaaaaacat cgcatcatca
     9181 cgatttctta cattataagt taggtattgc gaatgtagaa aagtcattca aattagatta
     9241 ccaagaagaa gaatttaacc aacgtcgtga tgaactattc gacgatgcta aagaattgta
     9301 tgaattttac acagatgtag aacaagcacc attaatcaat gatttaaatc atgggccaat
     9361 tgcatatatt ggtgcacgac atctcatttt agaagaattg gagaaaatgc taattcaatt
     9421 gtcaacattc catagttatc atgatttaga gtttctattt gtgacacgtg aagatgaagt
     9481 tgaaacattg aaatgggcac gttggttgcc acatatgaca ttgagagggc aaaacattag
     9541 aggatttgtt tacaatcaac gaacgcgtga ccaaatttta acgtcaattt atagcatgat
     9601 taaagaacgt atccaagctg tgcgtgaacg cagcagaagt aatgagcaaa ttattttcac
     9661 accgcaatta gtgtttgtca ttacagatat gtcattaatt attgatcatg tcattttaga
     9721 atatgtaaac caagatttat cagaatatgg tatttcatta atctttgttg aagatgtgat
     9781 tgaaagtttg ccagagcatg tagataccat tattgatatc aagtctcgta ctgaaggcga
     9841 actgattacg aaagaaaaag aattagttca attgaaattt acacctgaaa atattgataa
     9901 cgtcgataaa gaatatatcg cgcgacgttt ggcgaatttg atacacgtcg aacatttgaa
     9961 aaatgcaatt cctgatagta ttacattttt agagatgtat aacgtgaaag aagtagatca
    10021 gcttgatgtg gttaatcgat ggagacaaaa cgaaacatac aaaacgatgg cagtaccttt
    10081 aggtgtaaga ggtaaagatg atattttatc attgaactta catgaaaaag cacacgggcc
    10141 acatggttta gttgctggta ccactggttc agggaaatct gagattatcc aatcatacat
    10201 tttatcttta gctattaatt ttcaccctca tgaagttgca ttcctattga ttgactataa
    10261 aggtgggggt atggcgaact tatttaaaga tttagtccat ttagttggta cgattacaaa
    10321 cttagatggc gatgaagcga tgcgtgcctt aacatcaatc aaagccgaat tgagaaaacg
    10381 tcaacgttta ttcggagagc atgatgttaa ccatattaat caataccata agttatttaa
    10441 agaaggtatt gcgacagaac caatgccaca tttattcatt atttccgatg agtttgccga
    10501 attaaaatca gaacaacctg attttatgaa agaacttgta tcaacggcac gtattggacg
    10561 ttcgttaggt attcatttaa tacttgcgac acaaaaacca tcgggtgttg ttgatgacca
    10621 aatttggtct aactctaaat ttaagttggc attaaaagta caagatagac aagacagtaa
    10681 tgaaatttta aaaacaccag atgcagcaga cattacatta ccaggtcgtg cgtatttaca
    10741 agttggtaat aatgaaattt atgaattatt ccaatctgca tggagtggtg caacatatga
    10801 catcgaaggc gataaattag aagttgaaga taagacgatt tacatgatta atgactatgg
    10861 tcaacttcaa gcaatcaaca aagacttgag tggacttgaa gatgaagaaa cgaaagaaaa
    10921 tcaaactgag ttagaagcgg tcatagatca tatcgaatct attacaacac gattagaaat
    10981 cgaagaagtt aagcgtccat ggctaccacc attgccagaa aatgtatatc aagaagattt
    11041 agtagaaaca gatttcagaa aattatggtc agatgatgca aaagaagtgg aattaacatt
    11101 aggacttaaa gacgtaccag aagaacaata tcaaggaccg atggtattgc aattgaaaaa
    11161 agctgggcac atcgcgttaa tcggaagtcc aggatatggt agaacaacgt tcttacacaa
    11221 cattattttc gatgttgcaa gacaccatcg tcctgatcaa gcacacatgt acttgttcga
    11281 tttcggtacc aatggtttga tgccagttac agacatacca catgtcgctg attactttac
    11341 agtagatcaa gaagacaaga ttgctaaggc gatacgtata tttaatgatg aaattgatcg
    11401 tcgtaagaag attttaagtc agtatcgtgt cactagtatt tctgaatatc gaaaattaac
    11461 tggtgaaaca attccgcatg tctttattct tattgataac tttgacgcag taaaagattc
    11521 acctttccaa gaagtttttg aaaatatgat gattaaaatg acgcgtgaag ggctagcatt
    11581 agacatgcaa gtaaccttaa ctgcttcaag agctaacgct atgaaaacac caatgtacat
    11641 taatatgaaa acgcgtatcg caatgttttt atatgataaa tcagaggtgt cgaacgtagt
    11701 aggacagcaa aaatttgcgg ttaaagatgt tgtgggtcga gcattgttaa gtagtgatga
    11761 caacgtatca ttccatattg gccaaccatt taaacatgat gagaccaaat catataatga
    11821 tcaaattaat gatgaagtat cggcgatgac agaattttat aaaggtgaaa caccaaatga
    11881 tattcctatg atgccagatg aaattaaata tgaagattac agagaatcat taaacttacc
    11941 agatatagtt gcaaatggtg ctttaccaat tggattagat tatgaaggtg ttacactaca
    12001 aaaaattaaa ttaactgaac cagcaatgat ttcatcagaa aatccgagag aaattgcgca
    12061 tattgctgaa attatgatga aagaaattga catattaaat gaaaaatatg cgatttgtat
    12121 cgcagactca agtggagagt ttaaagctta taggcatcaa gtggctaact ttgccgaaga
    12181 aagagaagac attaaagcga ttcatcaact aatgattgaa gacttaaagc aaagagaaat
    12241 ggacggccca tttgaaaaag attcacttta tattatcaat gattttaaaa catttattga
    12301 ttgcacgtat attccggaag atgatgttaa aaagcttatt acaaaaggac cagaacttgg
    12361 cttgaacatt ttatttgtcg gcattcataa agaattaata gatgcttatg ataaacagat
    12421 tgatgttgca cgtaaaatga ttaaccaatt tagtataggt attcgtattt cagaccaaca
    12481 attctttaaa tttagattta ttcaacgaga acctgttatt aaagaaaatg aagcatatat
    12541 ggtcgcaaac caagcttatc aaaagattag atggtttaaa tagcaatgaa ttaaatagga
    12601 gggaggtatg ttatgaattt taatgatatt gaaacaatgg ttaagtcgaa atttaaagat
    12661 attaaaaagc atgctgaaga gattgcgcat gaaattgaag ttcgttctgg atatttaaga
    12721 aaagctgaac aatataagcg attagaattt aatttgagtt ttgcactaga tgatattgaa
    12781 agcacagcaa aggacgtaca aactgcaaaa tctagtgcta ataaggacag tgtaactgtt
    12841 aagggaaagg cgcccaatac gttatatatt gaaaaaagaa atttgatgaa acaaaagctt
    12901 gaaatgttgg gtgaagatat cgataaaaat aaagaatccc tccaaaaagc taaggaaatt
    12961 gctggcgaaa aggcaagtga atattttaat aaagcaatga attaatattg aggtgaagat
    13021 atgggtggat ataaaggtat taaagcagat ggtggcaagg ttgatcaagc gaaacaatta
    13081 gcggcaaaaa cagctaaaga tattgaagca tgtcaaaagc aaacgcaaca gctcgctgag
    13141 tatatcgaag gtagtgattg ggaaggacag ttcgccaata aggtgaaaga tgtgttactc
    13201 attatggcaa agtttcaaga agaattagta caaccgatgg ctgaccatca aaaagcaatt
    13261 gataacttaa gtcaaaatct agcgaaatac gatacattat caattaagca agggcttgat
    13321 agggtgaacc catgatgaaa gatgttaagc gaatagatta tttttcttac gaagaattaa
    13381 caattttagg tggtagtaaa ttgcctctcg taaattttga attgtttgat ccatcaaatt
    13441 ttgaagaagc taaagctgct ttaattgaaa aggaattagt aacagagaat gacaagttaa
    13501 ctgatgcagg ttttaaagtg gctacattag tcagagagta tattagcgcc attgtaaata
    13561 ttcgaattaa tgatatgtat tttgcaccat ttagctatga aaaagatgaa tatattttgt
    13621 taagccggtt taaaaataat gggtttcaaa tacgaattat caataaagac attgcatggt
    13681 ggtcgattgt acaatcatat cctttattga tgagacaaga aaagtccaat gattgggact
    13741 ttaaacaaat tgacgatgaa acattggaga acttaaataa tgaaagtatc gatacgattg
    13801 ggcgtgtttt agaaattgaa atatacaatc atcaaggtga ccctcaacaa agtttatata
    13861 acatttatga acaaaatgat ttgttattca ttcgataccc attaaaagat aaagtgctga
    13921 atgttcatat tggtgtcatt aatacattta tacgagaatt atttggattc gatactgatg
    13981 aaaatcatat taataaagca gaggagtaat gacgttgagt ggaaaaatta gtgttaaagc
    14041 tgaaacgatt gcacatgttg taaaagaatt ggaaagcata agtcaaaagt atgatgaaat
    14101 agctcaaaac tttggaaaaa tagcgcaatt aaattactac agtagtgaaa aagctgcaca
    14161 ttctatggaa aatggctata gtagtgctgc aacagtcatt agtggtctca aaggtccatt
    14221 gagtacactc ggtggtggcg tcatgaattc agcacaaaag ttctttgaag cagatgaaca
    14281 ttggggtacg gaatttgcca agctttacta taatattgag ggataggtgc atgacatgac
    14341 aaaagatatt gaatatctaa cagctgatta tgacaatgaa aagtcatcta tccaaagtgt
    14401 aatagatgca atagagggtc aagacttctt agatgtagat acaacaatgg atgatgcggt
    14461 aagcgatgtc agttctttag acgaagatgg cgcaatatca ttaacaagta gtgtagtagg
    14521 tccacaagga tctaaattaa tggggtatta tcaaaatgag ttatatgatt atgcatctca
    14581 attagattcg aaaatgaaag aaattattga cacgccattt atagaagata tagataaagc
    14641 attcaaaggt ataacgaatg ttaaattgga aaatatacta attaaaaatg gtggtggaca
    14701 tggtagagat acctatgggg cttctgggaa aattgcaaag ggagatgcca agaaaagtga
    14761 cagcgatgtt tatagcatcg atgaaatatt aaaatcggat caagaatttg taaaagtaat
    14821 tgatcagcat tacaaagaaa tgaaaaaaga agataagaaa ttatctaaaa gtgattttga
    14881 aaaaatgatg actcagggcg cttcttgtga ttacatgaca gtagctgaag cggaagagct
    14941 agaggagcaa aagaaaaaag aagaagctat agagattgca gcactagctg gtatggtagt
    15001 tttatcttgt attaatcctg ttgctggagc agtagctatt ggtgcttatt ccgcttattc
    15061 agcagcaaat gcagccacag gaaaaaatat tgtaactgga agaaagctat ctaaagaaga
    15121 acgaatcatg gaaggacttt cgcttattcc attgccaggt atgggcttcc tcaaaggtgc
    15181 tgggaaaagt ttaatgaaat taggcttcaa aggcggagaa aaatttgcag ttaaaacagg
    15241 attgcaaaag acaatgcaac aagcagttag tcgtatttca cctaaaatgg gaatgatgaa
    15301 aaacagtgtg ttgaatcaat ctcgtaactt tgctcaaaat actcatgttg gacaaatgct
    15361 gagtaacatg cgtggtcaag caactcatac tgttcaacaa agtagaaatt ggattggaca
    15421 acaagcacaa aatgtcaaac gaatagtgaa taatggactt gataaagaaa tagcacatcc
    15481 atttaaacaa caacttgcac cagcgggaat gggtggtata aaatttgctg aaacaactac
    15541 tttgagaaac atgggtcaaa acataaagcg tgctgttaca ccacaaaatc acgtgacaca
    15601 tggtccaaaa gatagtatgg tgagaagtga aggcaaacat agtataagta gccatgaaat
    15661 gaattcatca aagtatgttg aatcaccaaa ctacaccaag gttgaattcg gagaacacta
    15721 tgcaagactt agacctaaga aactaaaggc gaatattgaa tacacaacac ctactggtca
    15781 catatatcga accgatcata aaggtcgcat aaaagaagtt tatgtagaca atctctctct
    15841 aaaagatggg gatcgtaata gccatgcaca aagaactgtg gggggagagg atagattacc
    15901 agacgatgat ggaggtcact taatcgctag aatgtttggt ggttcaaaag acattgataa
    15961 ccttgtggca caaagtaaat ttatcaaccg tccatttaag gagaaaggtc attggtataa
    16021 tcttgagaaa gagtggcaag agttcttaaa ctctgggaaa gaggtgaaaa atattaaaat
    16081 ggaagtaaaa tatagcggta atagtcaaag accgactata tttaaagttg aatatgaaat
    16141 taatggtgaa agaaatatta gaagaatatt aaataagtag aggtgccaac atgacatttg
    16201 aagagaagct tagcaaaata tacaatga
//
LOCUS       DANJFZ010000058        16175 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.058, whole genome shotgun sequence.
ACCESSION   DANJFZ010000058 DANJFZ010000000
VERSION     DANJFZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.058"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..94
                     /locus_tag="PWN45_001601"
     CDS             <1..94
                     /locus_tag="PWN45_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HDL0623370.1"
                     /translation="LWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(187..348)
                     /locus_tag="PWN45_001602"
     CDS             complement(187..348)
                     /locus_tag="PWN45_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623371.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(385..1125)
                     /locus_tag="PWN45_001603"
     CDS             complement(385..1125)
                     /locus_tag="PWN45_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL0623372.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1331..2122
                     /locus_tag="PWN45_001604"
     CDS             1331..2122
                     /locus_tag="PWN45_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0623373.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2490..2918
                     /gene="lacA"
                     /locus_tag="PWN45_001605"
     CDS             2490..2918
                     /gene="lacA"
                     /locus_tag="PWN45_001605"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL0623374.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2934..3449
                     /gene="lacB"
                     /locus_tag="PWN45_001606"
     CDS             2934..3449
                     /gene="lacB"
                     /locus_tag="PWN45_001606"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL0623375.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3462..4394
                     /gene="lacC"
                     /locus_tag="PWN45_001607"
     CDS             3462..4394
                     /gene="lacC"
                     /locus_tag="PWN45_001607"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL0623376.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4398..5378
                     /gene="lacD"
                     /locus_tag="PWN45_001608"
     CDS             4398..5378
                     /gene="lacD"
                     /locus_tag="PWN45_001608"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0623377.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5400..5711
                     /locus_tag="PWN45_001609"
     CDS             5400..5711
                     /locus_tag="PWN45_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0623378.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5717..7429
                     /locus_tag="PWN45_001610"
     CDS             5717..7429
                     /locus_tag="PWN45_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0623379.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7447..8859
                     /gene="lacG"
                     /locus_tag="PWN45_001611"
     CDS             7447..8859
                     /gene="lacG"
                     /locus_tag="PWN45_001611"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0623380.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9125..9994
                     /locus_tag="PWN45_001612"
     CDS             9125..9994
                     /locus_tag="PWN45_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623381.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10277..11278
                     /locus_tag="PWN45_001613"
     CDS             10277..11278
                     /locus_tag="PWN45_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL0623382.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11512..12513
                     /locus_tag="PWN45_001614"
     CDS             11512..12513
                     /locus_tag="PWN45_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0623383.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            12820..14382
                     /locus_tag="PWN45_001615"
     CDS             12820..14382
                     /locus_tag="PWN45_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL0623384.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14557..15093
                     /gene="amaP"
                     /locus_tag="PWN45_001616"
     CDS             14557..15093
                     /gene="amaP"
                     /locus_tag="PWN45_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0623385.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15106..15345
                     /locus_tag="PWN45_001617"
     CDS             15106..15345
                     /locus_tag="PWN45_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0623386.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15408..15917
                     /locus_tag="PWN45_001618"
     CDS             15408..15917
                     /locus_tag="PWN45_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0623387.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(15953..>16175)
                     /locus_tag="PWN45_001619"
     CDS             complement(15953..>16175)
                     /locus_tag="PWN45_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0623388.1"
                     /translation="IDVEPVFGFMKAILGFTRMSVRGINKVKRELGFVLMALNIRKIA
                     AQRAVHYKIHIKKADFYQIINRNQLFYIA"
BASE COUNT         5699 a         2385 c         3069 g         5022 t
ORIGIN      
        1 tctatggcta gcttttaata ataatgaaga ttatgcaaga tattctgaaa aatataatga
       61 tttaatatcg gaatttgata atgaagatat ctaatttttt aatattcgta gcttttattt
      121 ttctacttat tttatgttta tttttaatct tacaaatgac aaaccattaa aagtagcatc
      181 ccaacatcaa acaaaaaaac aattcatcaa ataaaaatcg ctacaaaacc aagtcattaa
      241 acacgcaata attaaaattt tccactcatt ataattctga attccaaatg tcgaattccg
      301 aaaaccaaac tccaaattcc aaaaacgcaa ctccaaaatt aaaagcattt ccctaccatt
      361 cgggaaatgc tttttacata ctgattactc tgtcattaat gattttacaa cggaaaccat
      421 gtcgtcatgt atgaccaaag tagcgtcgct atcataaggt gttcgatctt tattgataat
      481 aattaaattg tcgcctttaa aatgtgatat taatcctgcg gcaggttgta caacgagtga
      541 tgaacctagt acaacaaggg tgtcagcatg ttcaatttta tttaatgccc ttatgatggt
      601 aggttgatct aacatttcac cgtataatac gatgtccggt cgaatggcac caccacaatt
      661 atcacaatgt ttcaaagttc tatcaataac atctgacttc gtataagatt tatgacatac
      721 attacaataa aaacgattta acgtgccatg taattcatca acatgttgac ttccagcgtc
      781 tgagtgcaaa ccatcgatat tttgcgtgat gacacctaaa gattgttgat tacgttctaa
      841 ttttgcaatc caatcatgaa cgatattggg catcgtatcg acaaatagta agcgcttatg
      901 gcagaaattg ataaaacctt caggatcatc ttctaaataa tcacggctta acaagtattc
      961 tggcgaaagc ccatcttttg aaatttcatc aaataagcca cccattgaac ggaaatctgg
     1021 aacgccactt gcgacagata caccagcacc tgtaaaaaat gtaatacgat tcgaactatc
     1081 tataatatgt tttagtgtct ctaaatcatg tttcaagtta aacaccccat caaaataatg
     1141 aatgatttat aacgtctttt acttttatta tagtatattc attttacgat tatcaaaagc
     1201 gcagtttaaa cagttctttt taaaaatgaa ttatacatat aatcgaacag ttaaattata
     1261 ttagttttta cgcaattttt attatagaat aagcatgtat attaaaattg atgtctaaac
     1321 ataaaggata atgacccatc gcctaaataa aggaggcaat tttacattga ataaacatga
     1381 acgtttggac gaaattgcta aactagtgaa taaaaagggc acgataagaa cgaatgaaat
     1441 cgtcgaaggt ttaaatgtgt ctgatatgac agttcgaaga gatttaattg aattggaaaa
     1501 taaagggatt ttaacgaaga ttcatggtgg tgcacgcagt aattcaacat ttcagtataa
     1561 agaaatatcg cataaagaaa aacatacccg tcaaattgca gaaaaacgat ttatagctaa
     1621 aaaagctgca tcattaattg aagatgggga tactttgttt ttcggaccag gaacaacagt
     1681 ggaactatta gcagaagaag tcaatcatca tacgctcaca attattacga attgtttgcc
     1741 ggtgtataaa attttgttag aaaaacaaac agcacatttt cgtgtctatt taattggtgg
     1801 tgaaatgcgc catattacag aagcatttgt aggtgaaatg gcgaacgcca tgttggaaaa
     1861 actaagattt agcaagatgt tctttagtag taacgcagta aataaaggtg ctgtgatgac
     1921 atctacatta gatgaagcct atacgcaaca actggcacta agtaattcaa ttgaaaaata
     1981 cttgttaatc gatcatacga aagttggcaa agaagatttt acatcatttt gtcagttaaa
     2041 cgaattgact gcagtggtca tggactatga agatgaagaa aaagtagaaa cgattaaaac
     2101 atatattgaa gtagtcgact aaattaaaag tataactgca ttgttaaaat atgtatctat
     2161 atgctcataa gtgaatgtga atttgcattc atttatgggc tattttttat gcaaatttat
     2221 gtcaacggga acctcgacaa agttttacta caatttcaga cgaataccta attaaaacaa
     2281 caccacaata tgaatgttca cttattgttt gaaaatacgt cataaacaaa catattataa
     2341 acatattatg ttgatttatt gtttgtttat gtttataatt taaacgtaat caaattgaaa
     2401 gccttttctc aagattttca taacctacaa ataaatatgt cacattttaa gaaaggtatt
     2461 tcaaaattaa agtaaaaagg agtcttatta tggcgattat tattggttca gatgaagctg
     2521 gcaaacgatt aaaagaagtc atcaaatcat acttattaga caataaatat gatgttgttg
     2581 acgtaacaga aggacaggaa gttgactttg ttgatgcaac tttggctgta gcaaaagatg
     2641 ttcaaagtca agaaggtaac ttaggtattg ttatcgatgc atttggtgca ggtagcttta
     2701 tggttgctac aaaaatcaaa ggcatgattg ctgctgaagt atcagatgaa cgctctggat
     2761 atatgactag aggccataac aattcaagaa tgatcactat gggatctgaa attgttggag
     2821 acacattagc gaaaaatgtc gttaaaggtt ttgttgaagg taaatacgat ggtggtagac
     2881 accaaatccg cgtagatatg cttaataaaa tgtgctaagg aggaaaaatt actatgaaga
     2941 ttgcattagg atgcgaccat attgttacag atacaaaaat gcgtgtatct gaatttttaa
     3001 aatcaaaagg acatgaagtc attgacgtag gaacatacga tttcacaaga acacattatc
     3061 caatttttgg taaaaaagtt ggcgaacaag ttgttagcgg taatgcagac ttaggtgttt
     3121 gtatttgtgg aacaggtgtt ggtattaaca atgctgtaaa taaagtacct ggcgttcgtt
     3181 cagcactagt acgtgatatg acatcagcgt tatacgctaa agaagaatta aatgcgaacg
     3241 ttattggctt cggtggacgt attataggtg agttattaat gtgcgatatt atcgatgcat
     3301 ttattaatgc tgaatataaa ccaactgaag agaacaaaaa attaatcgct aaaattaaac
     3361 atttagaaac aagcaatgca gatcaagctg atccacattt ctttgatgaa ttcttagaaa
     3421 aatgggacag aggcgaatac cacgattaag ggacgacaca tatgatttta actttaacat
     3481 taaatccatc cgttgatatt tcatacccat taacagcatt gaaattagat gatgttaatc
     3541 gtgttcaaga agtaagtaaa acagctggtg gtaaaggttt aaatgtaaca agagtacttg
     3601 cgcaagtagg tgagccagtc cttgcgagtg gttttattgg cggtgaatta ggtcaattta
     3661 ttgctaaaaa attagatcat gccgacatca agcatgcatt ttataacatt aagggtgaaa
     3721 cacgaaactg cattgcaatt ttacatgaag ggcaacaaac agaaatttta gagcagggac
     3781 cggagattga taatcaagag gctgcaggat ttattaaaca ttttgaacaa ttattagaaa
     3841 aagttgaagc agttgctatt tcaggtagct tacctaaagg attaaatcaa gattattatg
     3901 cacaaatcat tgaacgatgc caaaacaaag gtgtgcctgt gatattagat tgctcgggtg
     3961 caactttgca aacagttttg gaaaatccat acaaacctac agtcattaaa ccaaacattt
     4021 ctgaactata tcaattgctt aaccagccac ttgatgaaag tttagaaagt ttgaaacagg
     4081 cagtgtcaca acccttattc gaagggattg agtggattat tgtgtcttta ggtgcacaag
     4141 gtgcattcgc taagcacaat catacattct atagagtgaa tattccgaca ataagtgtat
     4201 taaatccggt tggttcaggt gattctacag ttgctggaat tacttcagca attcttaatc
     4261 atgaaaacga ccacgattta ctaaaaaaag ccaatacttt aggcatgctg aatgcacaag
     4321 aagcacaaac aggttatgtc aatttaaata attatgatga tttattcaat caaattgaag
     4381 ttttagaggt gtaaaaaatg tcaaaatcga atcaaaaaat cgcatcaatt gaacaattaa
     4441 gtaacaacga aggtatcatt tctgcattag catttgacca acgtggtgct ttaaaacgca
     4501 tgatggcaaa acatcaaact gaagaaccaa ctgtagctca aattgaacaa ttaaaagtat
     4561 tagttgcgga agaattaaca caatatgcat cttcaatttt attagatcct gaatatggtt
     4621 taccagcttc agatgcacgt aacaaagatt gtggattatt acttgcttat gaaaaaactg
     4681 gatatgacgt aaacgctaaa ggtcgtttac ctgactgttt agtagagtgg tctgcaaaac
     4741 gtttaaaaga acaaggcgca aatgctgtta aattcttact ttactatgat gttgatgatg
     4801 ctgaagaaat taacattcaa aagaaagcat acattgaaag aatcggttca gaatgtgttg
     4861 cagaagatat tccattcttt ttagaagtat taacatacga tgacaacatt cctgataacg
     4921 gtagtgttga attcgcaaaa gttaagccta gaaaggttaa cgaagcaatg aaattattct
     4981 cagaaccacg ctttaacgtt gatgtattga aagttgaagt accagtaaac atgaaatatg
     5041 tagaaggctt tgctgaaggt gaagtggttt atacaaaaga ggaagcggca caacacttta
     5101 aagatcaaga tgcagcaact catttaccat acatttactt aagtgctggt gtttctgcgg
     5161 aattattcca agaaacttta aaatttgctc acgaagctgg tgctaaattc aacggggtac
     5221 tttgtggccg tgctacatgg tctggtgctg ttcaagtata cattgaacaa ggtgaagatg
     5281 ctgctcgtga atggttacgt acaactggtt tcaaaaatat tgatgactta aataaagtat
     5341 taaaagatac tgcgacatct tggaaacaaa gaaaataagt ttaaaagggg attaatgcta
     5401 tgaatagaga agaagtccaa ttattaggtt ttgaaattgt tgcatttgca ggggatgcac
     5461 gttctaagtt tttagaagca ttaacagcag ctcaagctgg agattttgca aaagcagatg
     5521 cactgattga agaaggaaac aattgcattg cggaagcgca tagagcacaa acaagcctgt
     5581 tggctaaaga agcgcaaggt gatgatattg catacagtgt aacgatgatg catggtcaag
     5641 atcatttaat gacaacaatt ttactgaaag atttaatgaa gcatttatta gaattttata
     5701 aaagagggtg atgacaatga tgcaaaaatt gatcgcacaa attgaaaaag ggaagccatt
     5761 tttcgagaaa ttatcacgta acatatattt aagagccatc cgtgatggat ttatttctgc
     5821 aatgcctgtc atcttattct caagtatctt tctattaatt gcatatgtgc caaatatatt
     5881 tggttttaaa tgggataaag gcatggaagc cattttaatg aaaccataca actatacgat
     5941 ggggcttgta gcatttttag tagcaggaac gacagcgaaa tctttaacag attcatttaa
     6001 ccgtaagcta gaaagtacaa atcaaattaa ctttatttct acaatgctag cagcgatgtg
     6061 tggattcttg ttcttagctt ctgatccagc caaagatggt ggattcttaa gtgcatttat
     6121 gggaactaaa ggtttattaa cagcattctt atcagcattt gtaactgtta ttgtttataa
     6181 cttctgtgtg aagcgcaata ttacaattaa aatgccgaaa gaagtaccgc cgaatatttc
     6241 acaagtattt aaggacttaa ttccattttc agcggtaatc atcattcttt atgcattaga
     6301 tttagtcatt cgcaacagct ttaaatcaaa tgtagcggaa ggtattttaa aattattcga
     6361 accattattt acagcagcag atggatggat tggtgtcaca attatctttg gtgcctttgc
     6421 attattctgg tttgtaggta ttcatggtcc gtcaattgta gagccagcaa ttgcagccat
     6481 tacatatgcg aatatcgaag cgaacttcaa gttgcttcaa gctggagaac acgcagataa
     6541 aattattaca tcaggtacac aaatgtttat cgttacattt ggaggtacgg gtgcaacatt
     6601 ggtcgtcccg ttcatgttta tgtggatgac gaaatctaaa cgaaacaaag cgattggacg
     6661 tgcatcagtt gtaccaacat tctttggtgt aaacgaacca attttatttg gtgcaccact
     6721 agtattgaat cctgtgttct ttattccatt tgtattagca ccaattgtta acgtatggat
     6781 tttcaaactt ttcgttgaag tgttaggaat gaatagtttt agtgtgaatt taccttggac
     6841 aacaccaggt ccattgggca ttatcatggg tacaggtttt ggtttatggt cattcgtact
     6901 agctattact ttgattgttg tagatattat tatttactac ccattcctaa aagtttatga
     6961 tagtgaaatt cttgatgaag aagaaggacg taaagaaagt aattcagatt taaaagaaaa
     7021 agttgcagca aactttgata cgaaaaaagc tgattcaatt ttagcggcaa gtggtgtatc
     7081 agacgatgca gctaaagctt caaatatcac agaacaaaca aatgtcttag tactttgtgc
     7141 cggtggtggt acaagtggat tattagccaa tgcacttaat aaagcagctg aagaatatca
     7201 tgtgccagtg aaagcggcag ctggcggata tggcgcacat atggacatta tgaaagagta
     7261 ccaattaatc attttagcgc cacaagttgc gtctaactat gaagatatta agcaagatac
     7321 agatagatta ggtattaaat tagcgaagac tcaaggtgct gaatatatca aattgacacg
     7381 agatggccaa gcagcccttg atttcgttca acaacaattt gaaaattaag aataggagtt
     7441 tttcatatga ctaaaacatt acctgaagat tttatttttg gtggagcaac agcagcatat
     7501 caagcagaag gtgcgacaaa tacggatggt aaaggtcgcg tagcatggga tacgtattta
     7561 gaagaaaact attggtacac tgcagaacca gcgagtgatt tttataacag atatccagtg
     7621 gatttagaat taagtgaaaa attcggtgtt aatggtatcc gaatttcaat tgcatggtct
     7681 cgtatcttcc caaatggata tggcgaagtt aaccctaaag gtgttgagta ttaccataag
     7741 ttatttgcag agtgtcataa acgtcatgtt gaaccatttg taacattgca tcattttgat
     7801 acaccagaag tgttacacaa agatggcgat tttctaaatc gtaaaacgat tgactacttt
     7861 gtagattatg ctgaatattg ttttaaagaa ttcccagaag taaaatattg gactacattc
     7921 aatgaaattg gacctattgg tgatggtcaa tatttagtag gtaaattccc tccaggtatt
     7981 aaatatgatt ttgaaaaagt attccaatca catcataaca tgatggtggc tcatgctaga
     8041 gcagtaaaat tatttaaaga tggcggatac aaaggagaaa ttggtgttgt acacgcattg
     8101 ccaacaaaat atccgttcga tccatcaaat cctgaagatg ttcgtgcagc agaattagaa
     8161 gatatcatcc ataataaatt catcttagat gcaacatatt taggtaagta ttctcgtgaa
     8221 acgatggaag gtgtgcaaca tatcttatct gttaatggcg gtaaattaaa cattacagat
     8281 gaagattatg ccattttaga tgcagccaaa gatttaaacg acttcttagg tatcaattac
     8341 tacatgagtg attggatgag aggttacgat ggagaatctg aaatcacgca taatgcgaca
     8401 ggtgataaag gcggttctaa ataccaactt aaaggtgtcg gacaacgtga atttgatgtc
     8461 gacgtacctc gcacagactg ggactggatg atctatcctc aaggtttata tgatcaaatc
     8521 atgcgtgttg ttaaagatta tcctaactat cataagattt atatcactga aaatggttta
     8581 ggatataaag atgaatttat tgaatctgaa aaaacagttc atgatgatgc acgtattgat
     8641 tatgtaagac aacatttgaa cgttattgca gatgctatta tagatggtgc aaatgttaaa
     8701 ggttacttta tttggtcatt aatggatgtg ttctcttggt caaatggtta tgaaaaacga
     8761 tatggtttat tctatgtcga ttttgagact caagaacgtt atcctaagaa aagtgcttat
     8821 tggtacaaag agttagccga aacaaaagaa attaaataat tatagatatt aaaaggccta
     8881 tcagtcgagt gctggtaggt ctttatttat agaaagagat aaattaaaca tgcatataca
     8941 tatttatata ttaatcaatg ggtgacacac cattgaaagc taaagaagca atcaattaac
     9001 aaacgttaac ggacaacttt ccaaagtcat cttaaaagtt aatgatgtat tcaaaatcaa
     9061 ctaaaggaca tttttaatca catcctttat acttatataa aagacatgaa tgggagttaa
     9121 aagtgtgaag aaaatttttg taattattac gacattgtta gcggtcgcta ttattatagg
     9181 ctctattatt atggtggtgt ttagtcaacg acaggcacaa acatttaaaa tacaacaaca
     9241 gcaatttgtt aaaaagccaa ttccaacatt gttcttacat ggatttggtg gtagtgccaa
     9301 ttctgaaaag tttatggtga agcaagcaga aaaaagaggg gtaactaaag atatcatcac
     9361 tgcttacgtt tcaaaggatg gtgcagtgac atttaaaggg aaactaagaa aagatgcagt
     9421 gaatcctatt gtgaaaattg aacttgaaaa taacagacaa ggctatttgg ataagaatgc
     9481 tgcgtggttt aaaaatgtac tgactaagtt acaatctgaa tacaatttcg ataaattcaa
     9541 ctttgtcggt cactcaatgg ggaacttaac atttgcacaa tatatgatga catatggtaa
     9601 cgacaagtca ctgccacagt taaacaaaca agtgaatatt gcagggacat ttaatggtgt
     9661 tttaaatatg aatgaagatg tcaatgaaat tacagttgat aaagatggta aaccaagtcg
     9721 aatgaatcaa ccttatcagc aattacgcgt gctaaaagat atttataaag gtaaaggtat
     9781 cgaagtgcta aatatttatg gggatttgaa agatggtaca cattcagacg gcagagtgtc
     9841 taatagttct tcgaaatcat taaaatattt actgggaaat agtccgaaaa gttatcgaga
     9901 atctaaatat gaaggtgagc ctgcacaaca tagtcagctt catgagaatg aaaatgttgc
     9961 taatgagtta atcgacttct tatggaaaaa ataaagcaac tgctagtttt tctggggcaa
    10021 aaaagcttgt aatattagag cttttcaaaa attatctatt gtttgagagt ttccataaaa
    10081 tgtctaagtc gtaacatttt aaatttatga ttatagcaaa tataatgtgg ttgaagttac
    10141 atgaatgaat cagtgcttaa gtcaaaatat aacaactaag aatgtatttg catacagaca
    10201 tcacgaagac acaattcaaa cttaacgctt aagtaacaaa taagtaaaag tttcaaataa
    10261 agaaaggtga ttaatgatgc aaaataaaca aattcttttt aacaaaatac cagaaggcat
    10321 gcctcaagag gatacattca aaattgaaga gattgacacg cctaaacttg agagcgatgg
    10381 tgtacttgta caaacgctgt atatttcagt ggatccatat atgagaggac gtatgactaa
    10441 ggcagattcg tatgtacagc cttttgaaat tggtaaaccg attgttagcc atgtcgttgc
    10501 aaaagtaatt gattcgacac tcgcagatta taaaaaagga gatgtcgttg taggtatgtt
    10561 gccttggcga atcattaatc atgtccaagc ggatcaaatt actaaagttc caacgacaga
    10621 tgtaccactt gatttatatt taagtgtttt aggaatgcca ggacaaacag catatcatgg
    10681 tctgcttgat attggacaac ctaaagccgg tgatactgtt gtggtttcag ctgcatctgg
    10741 tgctgtaggt tcagttgtag gtcaaattgc taagattaaa ggttgccgtg tagtaggtat
    10801 tgcgggtggc gacaaaaaag taaattattt aacagagaca ctgggctttg acgctggtat
    10861 tgattataag aaagaggatt ttgcagaagc gttagcacaa gcagtaccag atggtattga
    10921 tgtttatttt gaaaatgttg gtggaacagt tggcgatgaa gtatttaagc atttaaatcg
    10981 ttttgcacgt gtaccagtat gtggcgcaat ttcgtcatat aatcatcctg aagccgatat
    11041 cggaccacgt attcaaggta cgcttattaa gaagcaagta atgatgagag gcttcttggt
    11101 aagtgaattc gctaatgctt ttaaagaagc tagtgaacag cttgcaacat gggttcaaga
    11161 aggtaaaatt cagtcacaag taaccattga agatggtttt gaaaatgcgc cacatgcatt
    11221 taaaaattta tttactggcg ataactttgg taaacaagtt attaaagtaa cggaataata
    11281 aagtatacgt tttaacaaac taactttgtt aattgaccaa aatttaataa ttttttatct
    11341 aaagtaaatt tgttcagcaa catggtagaa ataagtctaa aatttaatac gaaagtcgtc
    11401 tgtaattatt tatatattca aatgaaaaag gagtcatgat gctgcgatat aagcacacat
    11461 ggcttctatt ttgtataata agaaataagt tcataaatga ggtgaaacga tatgaaaatg
    11521 ataggatttg agaagccctt taaattggaa gaaggaaatt tatttaaagt atacgaacaa
    11581 agaaagccaa cgccagaaaa cgatgatatc ttagtcaaag taaatagcat tagtgtaaat
    11641 cctgtagata caaagcaacg acaaatggaa gtcactcaag cacctcgtgt acttgggttt
    11701 gatgctattg gtactgttga agctatcgga ccagacgtta cattatttag tccgggtgac
    11761 gttgtatttt atgctggatc gccaaataga caaggatcaa atgcgacata tcaattggtg
    11821 tcagaagcta tagttgcaaa agcaccacac aacataagtg ctaatgaagc ggttagtcta
    11881 ccgctaactg gtattacggc atatgaaacg ttttttgata catttaaaat ttctacgaat
    11941 cctgctgaaa acgaagggaa gtctgtctta atcattaatg gtgccggtgg tgtaggcagt
    12001 atagccactc aaattgctaa acggtatggt ttaactgtca ttacaacagc atcacgacaa
    12061 gaaacaacag agtggtgtga aaagatgggc gcagatatcg tattgaatca taaagaggat
    12121 ttagtgcgtc aatttaaaga aattccatta gtagattata tattttgcac ctataataca
    12181 gacttgtatt acaatactat gattgaactg ataaaaccat tagggcatat tacgactatc
    12241 gtagctttta atgaagatca agatttaaat gcattaaaat taaaaagtat aacatttaca
    12301 catgaattta tgtttgcgcg tccaattcat cgaacgcctg atatgattaa acaacatgag
    12361 tatttagaag atattacgaa aaatatagaa tcagggcatt accaaccaac aacgacacaa
    12421 gtttttgaag gtttatcacc tgaaaactta tatcaagcac atcaattgtt ggaaaaacaa
    12481 tcaatgattg gaaaattagt aattaatata tagattagta agtaaagtcg ccttatgcag
    12541 aaaatactgt ataggcgact tattttttgt gtctagcgac tattgattct aaacggaaat
    12601 atggcaactt tgcaggtatc aaatcaataa tgcactagaa aggcattact taaatacaat
    12661 aaaatacttt gttgctcaat gataaataat ttgagtgtgt tttatatatc atttttaaaa
    12721 gatgaggcat tattaattgt gtctatataa aattacaaat gattaaaatg ttgcagttat
    12781 gggtatgaac ttaccattac acagaaaggg tttgtattta tgaaaaataa atcagtaagt
    12841 ctagtgtttt gggtatcttt agtgatatgt acgatatttg ttgcttttgg agcaatattt
    12901 ccgaaacagt tagaaaaact cacgcaaaac attactacat ttattgcgct acatttttct
    12961 tggtattatt tattgctcgt tttagttatt ttatttgtct gtgtgtatat tttattttcg
    13021 agatacgcaa acattacatt aggtgaagaa ggcgaagatc ctgaattctc attgccgtct
    13081 tggtttgcta tgttatttag tgctggtatg ggaataggac ttgtgttctg gacaacggca
    13141 gaaccaatca gtcatgcctt caaattaacg cctattcata aagctggcac gcaatctgca
    13201 attaatgatg caatgcagtt ttcatttttc cattggggga ttcatgcatg ggcagtctat
    13261 ggtattgtcg cgctagtgtt tgcatatttt agctttcata aaggatatcc tggcttagtc
    13321 agcgctacat tgacgccttt attaggtgaa aaggcaatgc gtggaccttt aggtggtgcc
    13381 attgatgtat tggcagtcat agctacagta acaggcgttg ctgcaacatt aggtttcggt
    13441 gcattgcaaa taaacgaagg tttacatttt ttatttaatg taccgagtaa cttcacgatg
    13501 caagtaatct tgattgtcat tgcaaccata ttatttactt ggtcagcatg gtcaggtatc
    13561 gataaaggta tcaaaacgtt gagtaatatc aacatgttac ttgcttttgt agtacttatt
    13621 ggtcttttta ttgttgggcc aacgctatac attttaaata catttacgaa tggtctaggt
    13681 aattatattg caaatttctt tagtatgagt ttacgcattc cgacaggtgg tcaaaaattc
    13741 caatggttac aaaattggac aatcttttac tgggcatggt ggatatcttg ggcaccattt
    13801 gtaggtatat ttattgcacg tgtgtcaaaa ggacgtacga ttaaagaatt tattctaggt
    13861 gtactttttg tcccggcgtt agtttgcttt atattctttg cggtattcgg cgcgtcagca
    13921 atttacttac aagataatca tattgccgat attgctaaag ctgcgacaga aacagcaact
    13981 tttgcgacgc tacaacatta tccgctaggt tttgtattaa gcttaattac tttacttgtt
    14041 attatgattt tctttgttac atcagcggat tcggcaactt atgttttagg tatgttgagt
    14101 tcaagtggtg atattaatcc gaagtcattt gtcaaagtga gttggggtat cattatggca
    14161 ttatttgcaa tcatcatgat ttacacaggt ggtactcaag caattcaaaa tttattaatc
    14221 attgctgcgt taccattttc cgtggtcatt atagcgatga tatggtcgct tataaaatct
    14281 ttgagtgagg agaaaccgag aaatagtaat aaggttttaa ttaaacatcg tgacccagat
    14341 attttagaat atcgttcttc aaaacatatt gacgaaaatt aattaataat gaggttttat
    14401 ttacaaaaac tagggaatat attactaaat ggtacttcat cttatatatt aaaaataaat
    14461 ttaaagttat taatttatta tgtttaagaa aaaaactgat gggtacatat ttaatatgtt
    14521 tttaaattaa ctttaactaa ggaggtgtcg ttaggggtga aacgacttaa aaactttatc
    14581 cttggcttat taatagtagt aatcgtgggg ttcctgttat tcatgtatat ccaagatgga
    14641 cgtataactg aatatcaaga ctacttcctt caatttgaat ggttccaacc attacttatt
    14701 tcacttgctg cactattgat attaataggt cttatattag tatttagtat cttcaaacct
    14761 acgcatcgaa aacctggatt atacaaagac ttcgatgatg gtcatgttta cgtatctcgt
    14821 aaagctgttg aaaagacggc attcgatacg gttgcaaaat atgatcaagt aagacaacca
    14881 aatgtagttg cgaaactata taacaaaaaa aataaatctt atatcgacat taaaactgac
    14941 tttttcgtac caaataatgt tcaagttcaa tctttaactg aagcaattcg ttcggatatt
    15001 aaaaagaatg tagaatattt cacagaaatg ccagtacgta agttagaagt taatgtgaga
    15061 gatcagaaaa cttctggtcc acgagtgttg taagggaggt atatcatggc taacaatcat
    15121 aaccaaaacg gacaagactc tacacaacaa gtgattaatt ttttaaaagt atttaaatgg
    15181 agaatcgttg gtttcctagc gtttctatta atcgcgatat tattcttaac gttaggtttt
    15241 tggaaaacag tacttatcat cgttttatgt ttaattggtg taggtattgg gtatatgaaa
    15301 gaccgtaagc aagattttat gaatttttta aatcgatgga gttaattcat caatttagaa
    15361 tttaattaaa aaaatataaa aattaacaag taaaaggaga atctattatg actgtagata
    15421 acaataaagc aaaacaagca tacgacaatc aaactggtgt taacgaaaaa gaaagagaag
    15481 agcgtcaaaa acaacaagaa caaaatcagg agcctcaatt caaaaacaaa ttaacattct
    15541 ctgatgaagt tgttgaaaaa attgctggta tcgctgcacg tgaagttaaa ggtatcttag
    15601 atatgaaagg tggcttaact gatacattca ctaatgcatt ctcaagtggc aacaatgtta
    15661 ctcaaggtgt atctgttgaa gttggtgaaa aacaagctgc tgtagactta aaagtaatct
    15721 tagaatatgg tgaatcagca ccaaaaatct tccgtaaagt aactgaatta gttaaagaac
    15781 aagttaaata tattactggt ttagatgtag ttgaagttaa catgcaagtt gacgatgtaa
    15841 tgactcaaaa agagtggaaa caaaaacacg aaaaaaataa cgaaaacaat aaccaagaaa
    15901 gacaaggttt acaataagtt attcgataaa aagggcttgg gacattaaat tcttaggcaa
    15961 tgtaaaaaag ctgatttcta ttaattattt gatagaaatc agcttttttg atatgtattt
    16021 tataatgtac agctcgttga gctgctattt tccttatatt aagtgccatt aatacaaaac
    16081 ctagctctcg tttaacttta tttattcctc gaactgacat tcgagtgaaa cccaaaatag
    16141 ccttcataaa tccaaaaaca ggctctacat caatt
//
LOCUS       DANJFZ010000059        15832 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.059, whole genome shotgun sequence.
ACCESSION   DANJFZ010000059 DANJFZ010000000
VERSION     DANJFZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.059"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            14..784
                     /locus_tag="PWN45_001620"
     CDS             14..784
                     /locus_tag="PWN45_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0623389.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            836..1606
                     /locus_tag="PWN45_001621"
     CDS             836..1606
                     /locus_tag="PWN45_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0623390.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            1670..2440
                     /locus_tag="PWN45_001622"
     CDS             1670..2440
                     /locus_tag="PWN45_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0623391.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            2507..3274
                     /locus_tag="PWN45_001623"
     CDS             2507..3274
                     /locus_tag="PWN45_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0623392.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            3368..5605
                     /locus_tag="PWN45_001624"
     CDS             3368..5605
                     /locus_tag="PWN45_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0623393.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5756..6934
                     /locus_tag="PWN45_001625"
     CDS             5756..6934
                     /locus_tag="PWN45_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0623394.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6936..8324
                     /locus_tag="PWN45_001626"
     CDS             6936..8324
                     /locus_tag="PWN45_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623395.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(8817..10478)
                     /locus_tag="PWN45_001627"
     CDS             complement(8817..10478)
                     /locus_tag="PWN45_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDL0623396.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(10804..12579)
                     /locus_tag="PWN45_001628"
     CDS             complement(10804..12579)
                     /locus_tag="PWN45_001628"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL0623397.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(12753..13625)
                     /locus_tag="PWN45_001629"
     CDS             complement(12753..13625)
                     /locus_tag="PWN45_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL0623398.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            13738..15135
                     /locus_tag="PWN45_001630"
     CDS             13738..15135
                     /locus_tag="PWN45_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0623399.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            15325..15798
                     /locus_tag="PWN45_001631"
     CDS             15325..15798
                     /locus_tag="PWN45_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDL0623400.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         5630 a         2316 c         2851 g         5035 t
ORIGIN      
        1 agagagttgt aagatgatga aacgattaaa caaattagtg ttaggcatta tttttctgtt
       61 tttagtcatt agtatcactg ctggttgtgg cataggtaaa gaagcggaag ttaagaaaag
      121 ctttgaaaaa acattgagta tgtaccctat taaaaatcta gaggatttat acgataagga
      181 aggctatcgt gatgatcagt ttgataaaaa tgataaaggt acatggatta taaattctga
      241 aatggttatt caacctaata atgaagatat ggtagctaaa ggcatggttc tatatatgaa
      301 tagaaatacc aaaacaacaa atggttacta ctatgtcgat gtgactaagg acgaggatga
      361 aggaaaaccg cacgacaatg aaaaaagata tccggttaaa atggtcgata ataaaatcat
      421 tccaacaaaa gaaattaaag atgaaaaaat aaaaaaagaa atcgaaaact ttaagttctt
      481 tgttcaatat ggcgacttta aaaatttgaa aaattataaa gacggagata tttcatataa
      541 tccagaggtg ccgagttatt cggctaaata tcaattaact aatgatgatt ataatgtaaa
      601 acaattacgc aaaagatatg atataccgac gagtaaagct ccaaagttat tgttaaaagg
      661 ttcagggaat ttaaaaggct catcagttgg atataaagat attgaattta cgtttgtaga
      721 gaaaaaagaa gaaaatatat actttagtga tagcttagat tataaaaaaa gcggagatgt
      781 ataatcatgg ctcaatcaga atatgaaatc aatcccggaa aaagagagtg atgaaatgat
      841 aaaacgtgta aataaattag tgcttggtat tagtcttctg tttttagtca ttagtatcac
      901 tgctggttgt ggcatgggta aagaagcgga aataaagaaa agttttgaaa aaacattgag
      961 tatgtatccg attaaaaatc tagaggattt atacgataaa gaaggatatc gtgatgatca
     1021 atttgataaa aatgataagg gaacatggat tgtaaattct caaatggcaa ttcaaaataa
     1081 aggagaagct ctaaaaataa aaggcatgct tttgaagata gatagaaata caagaagtgc
     1141 aaaaggattt tactatacta atgaaataaa gacggagaaa tacgaagtag ctcaggataa
     1201 tcaaaaaaaa tatccagtta aaatgattaa taataaattc atttctactg aggaagttaa
     1261 agaagaaaac ataaaaaaag aaatcgaaaa ctttaagttt tttgcgcaat atagcaattt
     1321 taaagattta atgaattata aagatggaga tatatcatat aatccagagg tgccgagtta
     1381 ttcagctcaa tatcaattaa ctaatgatga ttataatgta aaacaattac gtaaaagata
     1441 tgacatacca acaaataaag cgccgaagct gttgttgaaa ggtacaggga atttaaaagg
     1501 ttcatcagtt ggatataaaa aaattgaatt tactttttta gagaataaaa atgaaaatat
     1561 ttactttact gatagtctac atcttgaacc gagcgaggat aaataatcat cttttaacca
     1621 gaatatgaaa tcaatctagg acaacctaat aaaaggagag agttgtaaga tgatgaaacg
     1681 attaaacaaa ttagtgttag gcattatttt tctgttttta gtcattagta tcactgctgg
     1741 ttgtggcata ggtaaagaag cgaaaataaa gaaaagtttt gagaagacat tgagtatgta
     1801 tccgattaaa aatctagagg atttatacga taaagaaggt tatcgtgatg acgaatttga
     1861 taaaaatgat aaaggtacat ggattattgg ttctgaaatg gcaactcaaa ataaggggga
     1921 agctctgaaa gttaaaggta tggtcttgta tatgaataga aataccaaaa caacaaaagg
     1981 atattattat gtaaatgcaa taaagaatga taaagacgga agaccccaag agaatgaaaa
     2041 aaggtaccca gttaaaatgg tcgataataa aatcattcca acaaaagaaa ttaaagataa
     2101 aaacataaaa aaagaaatcg aaaactttaa gttctttgtt caatatggaa actttaaaga
     2161 tttgtcgaag tataaagacg gagatatttc atataatcca gaggtgccga gttattcggc
     2221 taaatatcaa ttaactaatg atgattacaa tgtaaagcaa ttacgtaaaa gatatgacat
     2281 accaacaaat aaagcaccga agttgttgtt gaagggtaca gggaatttaa aaggttcatc
     2341 agttggatat aaagacattg aatttacgtt tgtagagaaa aaagaagaga acatttactt
     2401 tagtgatggg ttaattttta aaccaagtga ggataaataa tcatgactcg atcaaaatat
     2461 gaaatcaatc caggaaagat aataagaaga ataggattgt gaaattatga aacgattaaa
     2521 caaattggta ttgtatatta gttttttgat attagtcatt agtttcactg ctggttgtgg
     2581 cataggtaaa gaagcggaag ttaagaaaag ctttgaaaaa acattgagta tgtatccgat
     2641 taaaaatcta gaggatttat acgataaaga aggttatcgt gatgacgaat ttgataaaaa
     2701 tgataaaggt acatggatta ttggttctga aatggttgtt caacctaaag gggaacgtat
     2761 gaaatcaaaa ggtatggttc tatatatgaa tagaaacact aagactacaa ccgggaagta
     2821 tatagtgagt gaaacacttc atgatgaaga tggtagacct aagagtaaag ataaagaata
     2881 tcccgttaaa atggtagaca ataaaatcat tccaactaaa ggtatcaaag acgaaaatat
     2941 aaaaaaagaa atcgagaatt ttaagttctt cgcgcaatat ggcagcttta aagatttgtc
     3001 gaagtacaaa gacggcgata tatcatataa tccagaggta ccgagttatt cagcaaaata
     3061 tcaattaact aatgatgact ataatgtaaa acaattacga aaaagatata aaataccaac
     3121 gaataaagca ccaaagttat tgttgaaagg ttcgggagac ttaaaaggtt catcagttgg
     3181 atataaagac attgaattta cttttgtgga gaaaaaaggt gaaaatactt tttttactga
     3241 tagtctacat cttgaaccga gtgaggataa ataatcatcc acacacacga ttcaatatga
     3301 aattcaaata tgttgctgta aaataatgta aaataaacgt attcatatta ctgaattgag
     3361 ggatagtatg caacgtgatt atttaattcg agtagaaact gagagtatgt cagatttcaa
     3421 aaggctcaat agtttaatga ttggttttgt tattaaaggt gaggcacaca tttatgatga
     3481 aaataatatg acgcaatgca acagtggcga cattttcatc attaaccacc gcgacttgta
     3541 tcgatttcaa cttcaacaag atggcatcat atgttatatc caattccaaa tgaaatattt
     3601 agcagacaag tttgatgatg tgcattgtct atattttcac ttaacagatg cgaccacaac
     3661 aaagaatata catcaactga gaaatataat ggcaagactg gtttcaacac atatccgaca
     3721 taatgagttg tctaaattga ctgagcaaca acttgtgatt cagttgctta tgcatatgat
     3781 tcattatgtc ccgcgtacat atcattcgaa ccaaagtatc ttaaatgatg ataaagtgaa
     3841 tcaagtatgc gactatatcg agttacattt tcatgaagat ttaagccttt cagaattaag
     3901 cgaatacgtt gggtggtcag agagccatct gtctaaaaag tttacagaat cgctaggtgt
     3961 aggattccat catttcttaa atacgacgcg aattgagcat gcgaaactcg atttgacata
     4021 cacagatgaa accattactg atattgcatt gcaaaatggc ttttcaagtg cagcgagctt
     4081 tgcgagaaca tttaaacact ttacgcatca aacacctaaa caatatcgag gtgatcgtcc
     4141 agcaatcact gaaaaccagc aatcggcaca acatgattat cacgaccgtg aattgatatt
     4201 acttttaaat gactacattg aagaaatgaa tcattttatt gaagatattg aaaagatgaa
     4261 ctataaagag attgcctttc aaccaaccaa tcaccaacta aatcaattta atcatattat
     4321 tcaagtgggc tatttgagga atttgctcaa tacacagtat caatcacagt tgcttacatg
     4381 tcatcatgat tttcaagtca atgaagtatt agcatatgat gtgattccat atatcatgaa
     4441 aaagctcaat gcgccattca cgtatgatgc agaaatttcg aatatatttt atgatattga
     4501 tttgtgttta gactttttat tagatcataa ctttagtttg accatgcatt tggatcagta
     4561 tgactcacgt gattatatcg atgcatttaa aatttttatc catcacgttg ccctgcatgt
     4621 cagtcataga aaagatttga agttcaactt gtatgtgacg acattgcaca cgtctttgat
     4681 tgaaatgatt gattatttta aagcattatt ccctaatggt ggcttgtaca ttcacttaga
     4741 tcaagctacg gaaagacatc taccattatt gaaacgactt gagccacaca tcgaccattt
     4801 tgtatttgat gccaattcaa atgacgctgt tgattttaat aaaatgaatg atgatgaatt
     4861 taaaaccgcg agtcaaatga ttattaataa aacgaattac cttatcgact taatgcatcg
     4921 tcatcgccta aagcgtccac tcattttact caattggaat acattgacgg gtgatacatt
     4981 tattacaaac ggcgaatatt ttagaggtgg tatcatcatt gagcaattat taaaattaag
     5041 ttctaaagta gagggtatcg gttattggtt gaattatgat ttgcacgtca gtcattgtaa
     5101 aaatgaacgg gattatatga attctattga actatttcat caatataatg gaaaacgtcc
     5161 ggtctatttc acggcattgc tatttaataa attaacaagc aatattttgt attctgatga
     5221 tacatgtatt gtcacgggga ctgattcaaa ttttcaaata ttgttatatg atgcaaagca
     5281 ttttaatccg tacttagcgt tggacaatca aatgaatatg cgtgcgacgg aaatgattca
     5341 tttgaacatt aatgccctgg aagaaggtat gtataagatt aaacatttta ccttagataa
     5401 agaaaatggt gcgttattta atctttggcg caaacatcat acgatacatg gcatggacaa
     5461 ggactctata gattacgtta atcgaatgag ttttccgaaa ttagaagtat atgatataga
     5521 tatcacggac acactggcat taaacattaa aatgattacg aatgggattc acttaattga
     5581 agtaaaacgt tacccaagtt cataaaatga tcacaaatca caaattttga tatacataat
     5641 ttgtgatttt ttatattcaa agctaaaatt gcaaaaaatt aatggttaac atctctgttg
     5701 ttggcaatat aaataatgat taatcattta tgatgtaact aaggagatga aggatatgaa
     5761 tcaacaatta attgaaactt taaaatctaa agaaggcaaa atgattgaga tcagacgtta
     5821 tttacatcag catccagaat tatcttttca tgaagatgaa acggcgaaat acatcgctga
     5881 attttacaaa ggtaaagatg tggaagtaga aacgaatgtc ggaccacgtg gaattaaagt
     5941 aacgattgat tcagggaaac ctggtaaaac attagcaatc cgtgcagact ttgacgcatt
     6001 acccattact gaagatacag gattatcttt tgcatcacaa aataaaggtg ttatgcacgc
     6061 atgtggtcac gatgcacata cagcatacat gcttgtatta gcagagacgc ttgctgaaat
     6121 gaaagatagt tttacaggaa aagtcgttgt gatacatcaa ccagctgaag aagtaccacc
     6181 aggtggtgct aaaacaatga ttgaaaatgg tgtattagac ggtgttgatc atgtattagg
     6241 tgtacacgtc atgagcacaa tgaaaacagg taaagtgtat tacagacctg gttatgttca
     6301 aacaggacgc gcattcttca aattgaaagt tcaaggtaaa ggtggtcatg gttcatcacc
     6361 acatatggcc aatgatgcca ttgttgcagg tagctacttc gtcacagcgt tacaaacagt
     6421 tgtatctaga cgactaagtc catttgaaac cggtgttgtc acaatcggtt catttgacgg
     6481 taaaggtcaa ttcaatgtca ttaaagatgt tgttgaaatt gaaggtgatg tacgtggatt
     6541 aacagatgct acaaaagcaa caattgaaaa agaaattaaa cgtttatcaa aaggattaga
     6601 ggatatgtat ggtgtaactt gcaccttaga atataacgat gattatccag cattatataa
     6661 tgatccagag tttactgagt acgtggctaa gacgttgaaa gaagcaaacc ttgattttgg
     6721 tgttgaaatg tgtgaaccac aaccaccttc agaagacttt gcatattatg ctaaagaacg
     6781 cccaagtgcc tttatttata caggtgcagc ggtggaaaat ggtgaaattt acccacatca
     6841 tcatcctaaa tttaacattt cagaaaaatc attacttatt tctgcagaag cagtagggac
     6901 agttgtttta gattacctta aaggagataa ctaacatgaa tgaaacgtat cgcgggggca
     6961 acaagttaat cttaggtatt gtattaggtg ttattacatt ttggttgttt gcacaatcac
     7021 ttgtaaatgt tgtaccgaat ttacaacaaa gttttggtac agacatgggg acaattagta
     7081 ttgcggtcag tctaactgca ctattttcag gcatgtttgt tgttggagca ggtggtctgg
     7141 cagataaaat tgggcgcgtg aaaatgacga atatcggttt attattaagt attattggtt
     7201 cagcattaat tattattacg aatttaccgg cattattaat tttaggtcgt gttatacaag
     7261 gtgtatcagc agcgtgtatt atgccttcta cattggccat tatgaaaact tattatcagg
     7321 gtgctgaacg tcagcgtgcc ttaagttatt ggtctatcgg ttcttggggt ggcagtggta
     7381 tctgttcact cttcggtggt gcagttgcga caactatggg ttggagatgg attttcatct
     7441 tctcaattat cgttgccgta ctttcaatgt tactcatcaa agggacgcct gaaacgaaat
     7501 cagaaattac caatacacat aaatttgacg ttgcagggct aattgttcta gtagttatgt
     7561 tgctaagttt aaacgttgtc attactaaag gtgcagcact tggttacaca tcattatggt
     7621 tctttggttt gattgcaatc gtaattgtag cattctttat tttcttaaat gttgagaaaa
     7681 aagtagataa tccacttatt gattttaaat tatttgaaaa taaaccatat acaggtgcaa
     7741 cgatttcgaa cttcttatta aacggtgttg caggtacatt aattgtagcg aatacattcg
     7801 tgcaacaagg tttaggttat acagcattgc aggcaggata cttatcaatt acttatttaa
     7861 tcatggtgtt attgatgatt cgagttggtg aaaaattatt acaaaaaatg ggttctaagc
     7921 gaccaatgtt attaggtaca ttcattgtgg tcattggtat tgcacttatt tcattagtat
     7981 tcttaccagg catattttat gttatcagtt gtgtcgtagg atatttatgt ttcggactag
     8041 gcttaggtat ttatgcaaca ccttctacag atacagctat ttcgaatgca ccgttagata
     8101 aagttggcgt tgcttcaggt atttataaaa tggcttcatc acttggtggc gcattcggtg
     8161 tcgcaattag tggtgctgta tatgctggtg cagttgctgc aacgagcatt catacaggtg
     8221 cgatgattgc actttgggtt aacgtattaa tgggaatcat ggcatttatc gcaattttat
     8281 tcgcgattcc taatgatgat aaacgtgtca aagatgcgaa ataataacga tgacacgcca
     8341 agtgttttga taatgatata aatgaaagta aagcaaatta aatgaggaaa tgtatagagc
     8401 agtgcatata gataaatcta tgattggatt aacactaata taaatataaa tgaaaatgaa
     8461 aaaccatcca cttaaatttt tgataagtta ggtggatggt tatttattgc gttgttctaa
     8521 aaatttggac atttatatgg aaagaactat atataatgtg aaggttatgg caattcgctt
     8581 caatgaaggc gacgtgtaaa agtgtatatt aaacgtttcg gggtcagact ccgagaacag
     8641 tgtccgtcat gcttaccgca tactaatgaa gcattattat atcagtgttt atagccgatt
     8701 taagataata aattttgcta aacaaaaaag ccaacccatg aatgttggat tggcttttta
     8761 catgcatctg aatctctaat tttaaaaaaa tatgaatata aataagacag taaaaattaa
     8821 atttcagttg ttgcaatttc ttcatctgta ggtacatcat cgttaaggcc aacaagtgct
     8881 tcagaaacat ttcgtgaatg ataaccgata cgttcaagaa caccaatcat atcgatatat
     8941 agtaatccgc cttttgttgt acattcacca cgattaaggc gtttaatatg acctttgcgt
     9001 agtttatgtt caatattaaa tgattctcta ctacgttcta caatttcatc ttttttcgtt
     9061 ttgtcataaa catctaacat gtcgatggct ttatcaaatg actcagcaac atggttgaat
     9121 aatttatcca taccgcgttg tgcatcttct gtaatgcgaa tatcttcatc atgttggcgt
     9181 tttaattgag cgacatactc ttctgttagc tctgctactt ttaaaataga gcgattgaca
     9241 tcaaacataa ctgctaaacg ctcaacgtct gccttcgtaa tggcttttgt agaaattcta
     9301 actaaataat ttcgaatgct atcattgatt gtttcaacag cttgatgctt ttgttcaagc
     9361 tttttgatca attttttatc gtcttttgta atttcgcgaa tgtcttcaaa cattgataag
     9421 acaatctgac ccacattttg taattctttt tgagtttctt gtaatgcaac accaggtgcg
     9481 tgataaacaa gatctttgtt taagtgctga ggtttatagt catcagcaat atctttacct
     9541 gggacaagct ttgtaacaat ccatgctaaa cctgctacaa atggtaattg aatcaaagta
     9601 tttgttatgt tgaagatacc atgtgatact gcaatcgtca tcgctggttt taagtgccat
     9661 aaatcttgta acaaactaat caaatgaatc acaactggca agaaaattgt gaagataatt
     9721 accccgatta agttaaagat gacgtgtaca agcgccgcac gttttgcagc gattgagccg
     9781 gctaaactag ctaagatagc tgtaatcgtg gtaccaatgt tatcgcctag taacacaggg
     9841 attgctgcgt ttaagctaat taaatcttgt tgataaaatt cttgtaaaat accaatcgtc
     9901 gcacttgaac tttgaactag tgctgttaac cctgcgccga caatgacagc gagtattgga
     9961 tttgtagaca tatcaagcat taattgctta aatccatcta atgatgctaa aggtttaacg
    10021 gcatcaccca taaattctag accgaagaat agtgaaccga aaccgaatag tatgcggcca
    10081 atgttattga ttttagagcg tttaaagaaa aagattaaga atgcacctaa tgctaaaatt
    10141 ggcattgcat attcgcctaa atctataccg ataataaatg cagttaccgt tgttccgata
    10201 ttagcaccca ttatcactcc aatggcttgt ttcaatgtca taaatccagc tgttaccagt
    10261 ccgattgtga taactgtcgt acctgaacta ctttgtatta aaatagttac aacgatacct
    10321 gcaataacac ctaatactgg atttgatgta aatttgttta aaatatctcg tagcctgtct
    10381 cctgctgatg cttgaagccc gtctcccatg atttttaagc cgtaaaggaa aatacctaaa
    10441 ccacctaaaa aggagaaaat gacttctgta accgacattt ccattatttc acctcaaata
    10501 agctttatat ttagattatc gcttataatt gtaaatttaa tgttaagatt aggtaaaatt
    10561 atttaacaat atatgttatt tgtatatgac ttgtaaaata tcgtcactta ttatgtaaat
    10621 tttcagtgtg aaatggcagg tttgcaatca cttgtttaac aaaatgatgc aatcaatcat
    10681 gtaattatgt ttcatcaaaa aaatcatgag agtggaacaa cgaaataatt tttatgaaaa
    10741 catcatttct gtcccagtct catgatttga aatcaccaaa taaaaatcta ttaatggttt
    10801 tcgttataac aatttgtgtt cttttaataa tgactcaatg tacgtacctt ttatcttttt
    10861 aaggaatcct gctaatgcga gtttttgcat tttcgaatct ttagtaatct cacgcaaatc
    10921 ttggtggtca ttcagttcgt atatggcatc cattaagacg cgaagatcaa atggactatt
    10981 gatgacttct ggaataccac gatctatatt tagtaattga taaacagctt ccatggcagt
    11041 acgaaccgaa tattctgttg taaatacagt gtctcgctct gtttctgcaa agttaccaat
    11101 aaatgctaag ttctgagatt gatgcgggac gactaaaggt ctgtcgccga tagcacgcgt
    11161 catgaaataa gatgtgatat atggcatata aacaggaatc gtattagatg catgttttgc
    11221 taagtcttca attttgtcag ttgatacacc taagtgatac agccattctt ggcatatttc
    11281 attaccacta cattctgtaa ttggcttttt aatataatcg ccgtttacat ctgaatataa
    11341 ggcataaatc catgtagata tttcattttc aggttggtct ttaaactgtt gctgacgatt
    11401 gattgtaaaa ctcatttgcc atgcagaatc attgattgta ataataccgc ctgtaactgt
    11461 tttgcctgca agtgggtcac gtttacaaat actttctatt gtatcgataa tctctttatt
    11521 gtttgttgta gaagttgctg aaacaaacca acttttttta ggaatatttt ggcaaaactt
    11581 atcaggatta ccaaattcag gactttgtcg cgctaaattt ttccatagtg tccaactacc
    11641 acctaattcg tcagttggtg gcgctggtgt atcattatca ccataagtag agctttctgt
    11701 aatactaccg tttgtcacaa agacaagatc gtttatagtc agtttaatag attctgcatt
    11761 accattacgg tcaattaata tttctcgggc aattttttga cttgtcgtaa catctatttt
    11821 aatatcttcg acttttacat cgtattcaaa ttgaacccca tgcgatttta aatattcaac
    11881 cataggtaat actaaagatt catattgatt atatttagtg aattttaaag ctgaaaagtc
    11941 tgcgagacca ctaatatgat gaacgaatcg cattagatag cgacgcattt ccattgcaga
    12001 atgccacggt tcaaatgcaa acatcgtttt ccagtaaatc caaaagtttg aattaaagaa
    12061 gtcatcggaa aatacatctg ttattttgac atcatctaaa tcttcttcat tcgttaagca
    12121 taaatctaaa atttctttaa tcgccgtttt agtcaaagtg aagtctccgt ctgtgactaa
    12181 acgttgaccc tgtttctcaa taacacgaca gcgagaatag ttagggtctt ctttgtttag
    12241 ccaatagaac tcatctaata cagacgcgtt atcgatttct aatgaaggga tagatctgaa
    12301 taagtcccac aaacattcaa agtggttctc catttcacga ccaccgcgga caacatagcc
    12361 ttttaaaggc atattttcac catcaagact accacctgct ttaggtaact cttctaaaat
    12421 atgaatcttc gaaccttcca tttgaccatc ccttattaaa aaacaagctg cagcaagtga
    12481 agctagacca gatccgatta agtaagcgga tttgttttct acattttcag gttttttagg
    12541 gcgcgcaaat gcttcataat ttccataact gtaatacata tccaccaagt cctttcgtat
    12601 tagaatacac tcagactata cccctttgtg aattgcaaat attagtattt atattggtaa
    12661 atttgacgaa attagatcat ttttatattt ttgattttta aaattttatc actcgaatgt
    12721 ttttggattc tgaggtgcgg gacttggaat aattatgtat tttgaaagaa gttacttaaa
    12781 ctaacgccta gtagagtgat aattccgcct ataatagcaa gggttgttgg tagctcgtct
    12841 aataacagat aagataataa taaagaaacg ataggtgtta aataaagaga cattgttgca
    12901 tcagagacac caactgactt cacaatataa gcaagcaaaa cgtatggaat tatagtaggg
    12961 aatatagcta aataaagtac cgatactatt gatgtaaaag tggcgccgtg tatatcgttg
    13021 atgatttcag gaataaaaat aagcataaat ggtgagcttg ccattattgt atatagtgtg
    13081 aaagcgatga agccgtattt ttctatgtat tttttctgga aagtaaaata caaactttca
    13141 ctaaaagatg caagtaaaat aataaaaaca cctaatacat taatagttgt gtaatcatct
    13201 ttacttattg aaataatgga tattcctata aatgcgacga gtgaacttaa ccaattccat
    13261 tttgaaaaat gctcttttaa aaatatataa gctaaagcac tagaaaaaat aggcgttgta
    13321 gagactagaa ttccagatat acctgcacta atcaaagctt caccaaaatt taaagctgtg
    13381 tgatatatca caaatccaca aaatcctaaa ataaaaataa cagggatatc tcttagttca
    13441 ggggtaggca atttctttat aattacgaac ggcaagagaa ttattgttgc taaaattaaa
    13501 cgaaatgccg acaatgattc tgcactaaaa tcatttaacg caatctttat cattggaaat
    13561 gcagatcccc acaatatgat agtaaataaa tatgatagaa aagtagtgtc ttgaagttta
    13621 ttcattaata tcatcactcc tttaattatg tgtttctata ttaaaaaata tgatttaaaa
    13681 tgagtacaac caattttgaa tggatttacc tatccaattt taaaagggag ggagaagatg
    13741 gctaaatata aagatattgc tagtgacata agagataaaa taatcacagg ggattggttt
    13801 tatggaatga aaataccttc acataggcag ttggcgatac agtacaacgt aaatagagta
    13861 acgattatta aaagtattga gttattagaa gctgaaggat ttatctatac taaagtaggt
    13921 agtggaacat atgttaatga ctatttgaat gaagcacata ttacaaataa gtggtctgaa
    13981 atgatgttat ggtcctctca acaaagaagt cagtatacgg tgcaattaat taataaaatt
    14041 gagacagatg attcgtatat acatataagt aaaggtgaat tgggtatatc gttaatgcca
    14101 catattcaat tgaaaaaagc catgtctaat acagccagtc atattgaaga cttatctttt
    14161 ggttataata atggctatgg ttatatcaag ttaagagata ttatcgttga acgaatgtca
    14221 aagcaaggta taaatgtagg tagagaaaat gtaatgatca cttcaggcgc tttacatgcc
    14281 attcaacttt tatctattgg gtttttaggt caagatgcca taataatttc gaatacacca
    14341 tcatatattc actctacaaa tgtttttgag caattgaatt ttagacatat tgatgttcct
    14401 tataatcaaa ttaatgaaat tgataccatc attgatagat ttattaattt taaaaataaa
    14461 gcgatttata tagaacctag gtttaataac ccgacaggtc gttctttaac gaatgagcaa
    14521 aagaaaaata taattactta tagcgaaaga cataatattc ctatcattga agatgatatc
    14581 tttagagata ttttctttag cgatccaact ccttctatca aaacttatga taaattggga
    14641 aaagttatac atataagcag tttttcaaaa acgattgcac cagcaataag aataggttgg
    14701 attgttgctt ctgaaaaaat aatagagcaa ttggcagatg taagaatgca aattgactat
    14761 ggatccagta ttttgtcaca aatggttgta tatgagatgt tgaaaaataa gtcttatgat
    14821 aaacacttag taaagttaag gtatgtttta aaagataaac gagactttat gttaaacatc
    14881 ctcaataatt tatttaagga tatagcacat tgggaggttc caagtggagg ttattttgta
    14941 tggttagtct ttaaaataga tatagatatt aaatatttat tttacgaatt gttaagtaaa
    15001 gaaaaaatat taatcaatcc gggttacatt tatggcagta aagaaaagag tataaggcta
    15061 tcttttgcct ttgaatcaaa tgaaaatatt aagcatgcgc tctataaaat ttatacatat
    15121 gtgaaaaagg tttaattaaa acaataattc gaatcattat gtggcatgtt aaacagctaa
    15181 atataagcta tgcacattta acaagcggat actcctgacg caattatagt atcattacat
    15241 attgtttaat cgtttggggt aatgtaatta aaaggtaaaa taggcagagt gttatacata
    15301 aaatgtaaag aaagaggcga tagaatgtct aaaatcaggt cttttacaat attaagtcta
    15361 cttatttact tagctatgat gtgctataca gtagtgacct atccaaaatt accaaccaaa
    15421 gtgcctattc attataattt agcaggggat gctgataatt ttgctgataa atgggtgctg
    15481 cttttgatta atagcgcatt tatagtgatt tggcttatat ttttcattgc aggtagatac
    15541 tatgaacgat ttgccaaatg gtcacattat aatcatacac cacgtgaaat tcgagcgatt
    15601 aaattatttt taagtacgtt aaatttagag attatgagct atatgtctat cttcacagta
    15661 ttagaaattt ggcaaataca acaccatcat caattcaatt tactatggtt taatatgata
    15721 tttatcatta tcattggttt gacgcttgtc atattttgtt tacttcctac aattcataaa
    15781 atgagagatt ctcaataaaa atataaactc tcatgatgca gttttgaagc ga
//
LOCUS       DANJFZ010000060        15276 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.060, whole genome shotgun sequence.
ACCESSION   DANJFZ010000060 DANJFZ010000000
VERSION     DANJFZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.060"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..200
                     /locus_tag="PWN45_001632"
     CDS             <1..200
                     /locus_tag="PWN45_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HDL0623401.1"
                     /translation="DYPDPELLIRTSGEQRISNFLIWQVSYSEFIFNQKLWPDFDEDE
                     LIKCIKIYQSRQRRFGGLSEE"
     gene            207..989
                     /locus_tag="PWN45_001633"
     CDS             207..989
                     /locus_tag="PWN45_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL0623402.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            1251..2537
                     /gene="rseP"
                     /locus_tag="PWN45_001634"
     CDS             1251..2537
                     /gene="rseP"
                     /locus_tag="PWN45_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL0623403.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            2557..4260
                     /locus_tag="PWN45_001635"
     CDS             2557..4260
                     /locus_tag="PWN45_001635"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL0623404.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            4524..8834
                     /locus_tag="PWN45_001636"
     CDS             4524..8834
                     /locus_tag="PWN45_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL0623405.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            9124..9591
                     /gene="rimP"
                     /locus_tag="PWN45_001637"
     CDS             9124..9591
                     /gene="rimP"
                     /locus_tag="PWN45_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL0623406.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            9612..10787
                     /gene="nusA"
                     /locus_tag="PWN45_001638"
     CDS             9612..10787
                     /gene="nusA"
                     /locus_tag="PWN45_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL0623407.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            10808..11092
                     /locus_tag="PWN45_001639"
     CDS             10808..11092
                     /locus_tag="PWN45_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL0623408.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11089..11406
                     /locus_tag="PWN45_001640"
     CDS             11089..11406
                     /locus_tag="PWN45_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDL0623409.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            11411..13528
                     /gene="infB"
                     /locus_tag="PWN45_001641"
     CDS             11411..13528
                     /gene="infB"
                     /locus_tag="PWN45_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL0623410.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            13914..14264
                     /gene="rbfA"
                     /locus_tag="PWN45_001642"
     CDS             13914..14264
                     /gene="rbfA"
                     /locus_tag="PWN45_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL0623411.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            14433..>15276
                     /gene="truB"
                     /locus_tag="PWN45_001643"
     CDS             14433..>15276
                     /gene="truB"
                     /locus_tag="PWN45_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL0623412.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDS"
BASE COUNT         5523 a         2053 c         3039 g         4661 t
ORIGIN      
        1 aagactatcc tgatccagag ttgttaattc gtacttcagg agaacaaaga ataagtaatt
       61 tcttgatttg gcaagtttcg tatagtgaat ttatctttaa tcaaaaatta tggcctgact
      121 ttgacgaaga tgaattaatt aaatgtataa aaatttatca gtcacgtcaa agacgctttg
      181 gcggattgag tgaggagtag tatagtatga aagttagaac gctgacagct attattgcct
      241 taatcgtatt cttgcctatc ttgttaaaag gcggccttgt gttaatgata tttgctaata
      301 tattagcatt gattgcatta aaagaattgt tgaatatgaa tatgattaaa tttgtttcag
      361 ttcctggttt aattagtgca gttggtctta tcatcattat gttgccacaa catgcagggc
      421 catgggtaca agtaattcaa ttaaaaagtt taattgcaat gagctttatt gtattaagtt
      481 atactgtctt atctaaaaac agatttagtt ttatggatgc tgcattttgc ttaatgtctg
      541 tggcttatgt aggcattggt tttatgttct tttatgaaac gagatcagaa ggattacatt
      601 acatattata tgccttttta attgtttggc ttacagatac aggggcttac ttgtttggta
      661 aaatgatggg taaacataag ctttggccag taataagtcc gaataaaaca atcgaaggat
      721 tcataggtgg cttgttctgt agtttgatag taccacttgc aatgttatat tttgtagatt
      781 tcaatatgaa tgtatggata ttacttggag tgacattgat tttaagttta tttggtcaat
      841 taggtgattt agtggaatca ggatttaagc gtcatttcgg cgttaaagac tcaggtcgaa
      901 tactacctgg acacggtggt attttagacc gatttgacag ctttatgttt gtgttaccat
      961 tattaaatat tttattaata caatcttaat gctgagaaca aatcaataaa cgtaaagagg
     1021 agttgctgag ataatttaat gaatctcaga actccttttg aaaattatac gcaatattaa
     1081 ctttgaaaat tatacgcaat attaactttg aaaattatac gcaatattaa ctttgaaaat
     1141 tagacgttat attttgtgat ttgtcagtat catattataa tgacttatgt tacgtataca
     1201 gcaatcattt ttaaaataaa agaaatttat aaacaatcga ggtgtagcga gtgagctatt
     1261 tagttacaat aattgcattt attattgttt ttggtgtact agtaactgtt catgaatatg
     1321 gccatatgtt ttttgcgaaa agagcaggca ttatgtgtcc agaatttgcg atcggtatgg
     1381 ggccaaaaat ttttagtttt agaaaaaatg aaacacttta cactattagg ttattgcctg
     1441 ttggtggata tgttcgtatg gcaggagatg gcttagaaga gccaccagtc gagcccggta
     1501 tgaacgttaa aattaaactt aatgaagaaa atgaaataac acatatcata ttagatgatc
     1561 atcataagtt tcaacaaatt gaagcgatcg aagttaaaaa atgtgatttt aaggatgact
     1621 tattcataga aggtatcact gcttatgata atgaaagaca tcattttaaa attgctagaa
     1681 agtctttctt tgttgaaaat ggtagcttag ttcaaattgc tccgagagac agacaatttg
     1741 cacataaaaa gccatggccg aaatttttaa cattatttgc gggaccgtta tttaacttta
     1801 tattagcttt agtcctattt attggtcttg catattatca aggcacgcct acgtctactg
     1861 tagaacaagt cgcagataag tatccagctc aacaagcagg attacaaaaa ggtgataaga
     1921 tcgtccaaat tggcaaatat aaaatatctg aatttgatga tgttgataag gcgttagata
     1981 aagttaaaga taataagacg actgttaaat ttgaacgtga tggtaaaaca aagtcagttg
     2041 aattaacacc taaaaagact gaaaaaaaac tgactaaagt aagttcagag acgaagtatg
     2101 ttctcggatt ccaaccagcg agtgaacata cactttttaa accaattgta ttcggattta
     2161 aaagcttttt aatcggtagt acttatattt ttacagctgt agtaggtatg ttggctagta
     2221 tatttacggg cggattctca tttgatatgt taaatggtcc ggttggtatt tatcataacg
     2281 tcgactcagt tgttaaagcg ggtatcatta gcttaattgg ttacactgcg ttattaagtg
     2341 taaacttagg tattatgaat ttaattccta ttcctgcact agacggtggt cgtattttat
     2401 ttgttatata tgaagcgatt ttcagaaaac cagttaataa aaaagcggaa acaacgatta
     2461 ttgctattgg tgccattttc atggtcgtta taatgatatt agtaacgtgg aatgatattc
     2521 gacgatattt cttataattt aggaggataa ataattatga agcaatccaa agtttttata
     2581 ccaacgatgc gtgacgtgcc atcagaagca gaagcacaaa gtcatcgttt attattgaaa
     2641 tcgggtttga taaaacaaag tacaagtggg atttatagtt atttaccgct agcaacacgt
     2701 gtgttaaata atattactgc aattgtgcga caagaaatgg aacgtatcga ttctgttgaa
     2761 attttaatgc cagcgttaca acaagctgaa ttatgggaag aatcaggacg ttggggtgca
     2821 tatggcccag aattaatgcg tttacaagat agacatggaa gacaatttgc attaggtcca
     2881 acacatgaag aattagttac atcaatagta agaaatgaat tgaaatcata caaacaatta
     2941 ccgatgacat tattccaaat tcaatctaaa ttccgtgatg aaaagagacc acgttttggt
     3001 ttacttcgtg ggcgtgaatt tattatgaaa gatgcatatt cattccatgc tgacgaggca
     3061 tcattagatc aaacgtatca agatatgtat caagcgtata gccgtatttt tgagagagtt
     3121 ggcattaacg caagaccagt agttgcagat tcaggtgcta taggcggtag ccatacacat
     3181 gaatttatgg cattaagtgc tatcggtgag gatacaatcg tttacagtaa agaaagtgat
     3241 tatgctgcta acatcgaaaa agcagaagtc gtttacgaac caaatcataa gcatactact
     3301 gtgcaacctt tagaaaaaat tgaaacacca aatgttaaga ctgcgcaaga attggcagac
     3361 ttcttaggta gaccagtaga tgaaatcgtt aaaacgatga ttttcaaagt tgatggcgaa
     3421 tatattatgg ttttagtgcg tggccatcat gaaattaatg acattaaatt aaaatcttat
     3481 ttcggcacag ataatattga attagcaaca caagacgaaa ttgttaattt agttggtgca
     3541 aatcctggtt cactaggtcc tgtaattgat aaagaaatca aaatttatgc agataatttt
     3601 gtgcaagatt taaataattt agttgtcggt gctaacgaag atggttatca cttaattaat
     3661 gtaaatgtag gtagagactt caacgttgat gaatatggcg atttccgttt tattttagaa
     3721 ggcgaaaagt taagtgatgg ttcaggcgtt gcacattttg ctgaaggtat tgaagttggt
     3781 caagtattca aattgggtac taagtattca gaatcaatga atgctacatt cttagataac
     3841 caaggaaaag ctcaatcttt aattatgggt tgttacggaa ttggaatttc tagaacgcta
     3901 agtgcgattg ttgaacaaaa tcacgatgat aatggaattg tttggcctaa atcagttact
     3961 ccgtttgatt tacatttaat ttctattaat cctaagaaag atgatcaacg agaactagca
     4021 gatgcactat atgctgaatt taatactaaa tttgatgtgt tgtacgatga tcgtcaggaa
     4081 cgtgcaggtg ttaaatttaa tgatgccgat ttaattggtt taccactgcg aattgttgtt
     4141 ggtaaacgtg catcggaagg tattgtagaa gttaaagaac gtttaacagg tgatagcgaa
     4201 gaagttcaca ttgatgactt aatgactgtc attacaaata aatatgataa cttaaaataa
     4261 ttaagatcga atgaattata agagtaggaa aaagctgaaa gaaatctgat gcttatgtcc
     4321 tgctcttatt atttttgata taatgattat tcgatgaaaa atgactgaag acatagtata
     4381 attaaagata aatttgtttt aacaatataa tgattagcca aatataaagc atttaatttt
     4441 ctatcattac tatgctcaca taatctaaat attgttcgaa cacgtaaaag taatttctat
     4501 ttaaggtggt aattgtcttg gcaatgacag agcaacaaaa atttaaagtg cttgctgatc
     4561 aaattaaaat ttcaaatcaa ttagatgctg aaattttaaa ttcaggtgaa ctgacacgta
     4621 tagatgtttc taacaaaaac agaacatggg aatttcatat tacattacca caattcttag
     4681 ctcatgaaga ttatttatta tttataaatg caatagagca agagtttaaa gatatcgcca
     4741 acgttacatg tcgttttacg gtaacaaatg gcacgaatca agatgaacat gcaattaaat
     4801 actttgggca ctgtattgac caaacagctt tatctccaaa agttaaaggt caattgaaac
     4861 agaaaaagct tattatgtct ggaaaagtat taaaagtaat ggtatcaaat gacattgaac
     4921 gtaatcattt tgataaggca tgtaatggaa gtcttatcaa agcgtttaga aattgtggtt
     4981 ttgatatcga taaaatcata ttcgaaacaa atgataatga tcaagaacaa aacttagctt
     5041 ctttagaagc acatattcaa gaagaagacg aacaaagtgc acgattggca acagagaaac
     5101 ttgaaaaaat gaaagctgaa aaagcgaaac aacaagataa caacgaaagt gctgtcgata
     5161 agtgtcaaat tggtaagccg attcaaattg aaaatattaa accaattgaa tctattattg
     5221 aggaagagtt taaagttgca atagagggtg tcatttttga tataaactta aaagaactta
     5281 aaagtggtcg ccatatcgta gaaattaaag tgactgacta tacggactct ttagttttaa
     5341 aaatgtttac tcgtaaaaac aaagatgatt tagaacattt taaagcgcta agtgttggta
     5401 aatgggttag ggctcaaggt cgtattgaag aagatacatt tattagagat ttagttatga
     5461 tgatgtctga tattgaagag attaaaaaag cgacaaaaaa agataaggct gaagaaaagc
     5521 gtgtagaatt ccacttgcat actgcaatga gccaaatgga tggtataccc aatattggtg
     5581 cgtatgttaa acaggcagca gactggggac atccagccat tgcggttaca gaccataatg
     5641 ttgtgcaagc atttccagat gctcacgcag cagcggaaaa acatggcatt aaaatgatat
     5701 acggtatgga aggtatgtta gttgatgatg gtgttccgat tgcatacaaa ccacaagatg
     5761 tcgtattaaa agatgctact tatgttgtgt tcgacgttga gacaactggt ttatcaaatc
     5821 agtatgataa aatcatcgag cttgcagctg tgaaagttca taacggtgaa atcatcgata
     5881 agtttgaaag gtttagtaat ccgcatgaac gattatcgga aacgattatc aatttgacgc
     5941 atattactga tgatatgtta gtagatgccc ctgagattga agaagtactt acagagttta
     6001 aagaatgggt tggcgatgcg atattcgtag cgcataatgc ttcgtttgat atgggcttca
     6061 tcgatacggg atatgaacgt cttgggtttg gaccatcaac gaatggtgtt atcgatactt
     6121 tagaattatc tcgtacgatt aatactgaat atggtaaaca tggtttgaat ttcttggcta
     6181 aaaaatatgg cgtagaatta acgcaacatc accgtgccat ttatgataca gaagcaacag
     6241 cttacatttt cataaaaatg gttcaacaaa tgaaagaatt aggcgtatta aatcataacg
     6301 aaatcaacaa aaaactcagt aatgaagatg catataaacg tgcaagacct agtcatgtca
     6361 cattaattgt acaaaaccaa caaggtctta aaaatctatt taaaattgta agtgcatcat
     6421 tggtgaagta tttctaccgt acacctcgaa ttccacgttc attgttagat gaatatcgtg
     6481 agggattatt ggtaggtaca gcgtgtgatg aaggtgaatt atttacggca gttatgcaga
     6541 aggaccagag tcaagttgaa aaaattgcca aatattatga ttttattgaa attcaaccac
     6601 cggcacttta tcaagattta attgatagag agcttattag agatactgaa acattacatg
     6661 aaatttatca acgtttaata catgcaggtg acacagcggg tatacctgtt attgcgacag
     6721 gaaatgcaca ctatttgttt gaacatgatg gtatcgcacg taaaatttta atagcatcac
     6781 aacccggcaa tccacttaat cgctcaactt taccggaagc acattttaga actacagatg
     6841 aaatgttaaa cgagtttcat tttttaggtg aagaaaaagc gcatgaaatt gttgtgaaaa
     6901 atacaaacga attagcagat cgaattgaac gtgttgttcc tattaaagat gaattataca
     6961 caccgcgtat ggaaggtgct aacgaagaaa ttagagaact aagttatgca aatgcgcgta
     7021 aactgtatgg tgaagacctg cctcaaatcg taattgatcg attagaaaaa gaattaaaaa
     7081 gtattatcgg taatggattt gcggtaattt acttaatttc gcaacgttta gttaaaaaat
     7141 cattagatga tggatactta gttggttccc gtggttcagt aggttctagt tttgtagcga
     7201 caatgactga gattactgaa gtaaacccgt taccgccaca ctatatttgt ccgaactgta
     7261 aaacgagtga atttttcaat gatggttcag taggatcagg atttgattta cctgataaga
     7321 cgtgtgaaac ttgtggagcg ccacttatta aagaaggaca agatattccg tttgaaacat
     7381 ttttaggatt taagggagat aaagttcctg atatcgactt aaactttagt ggtgaatatc
     7441 aaccgaatgc ccataactac acaaaagtat tatttggtga ggataaagta ttccgtgcag
     7501 gtacaattgg tactgttgct gaaaagactg cttttggtta tgttaaaggt tatttgaatg
     7561 atcaaggtat ccacaaaaga ggtgctgaaa tagatcgact cgttaaagga tgtacaggtg
     7621 ttaaacgtac aactggacag catccagggg gtattattgt agtacctgat tacatggata
     7681 tttatgattt tacgccgata caatatcctg ccgatgatca aaattcagca tggatgacga
     7741 cacattttga tttccattct attcatgata atgtattaaa acttgatata cttggacacg
     7801 atgatccaac aatgattcgt atgcttcaag atttatcagg aattgatcca aaaacaatac
     7861 ctgtagatga taaagaagtt atgcagatat ttagtacacc tgaaagtttg ggtgttactg
     7921 aagatgaaat tttatgtaaa acaggtacat ttggggtacc agaattcggt acaggattcg
     7981 tgcgtcaaat gttagaagat acaaagccaa caacattttc tgaattagtt caaatctcag
     8041 gattatctca tggtacagat gtgtggttag gcaatgctca agaattaatt aaaaccggta
     8101 tatgtgattt atcaagtgta attggttgtc gtgatgatat catggtttat ttaatgtatg
     8161 ctggtttaga accatcaatg gcttttaaaa taatggagtc agtacgtaaa ggtaaaggtt
     8221 taactgaaga aatgattgaa acgatgaaag aaaatgaagt gccagattgg tatttagatt
     8281 catgtcttaa aattaagtac atgttcccta aagcccatgc agcagcatac gttttaatgg
     8341 cagtacgtat cgcatatttc aaagtacatc atccacttta ttactatgca tcttacttta
     8401 caattcgtgc gtcagacttt gatttaatca cgatgattaa agataaaaca agcattcgaa
     8461 atactgtaaa agacatgtat tctcgctata tggatctagg taaaaaagaa aaagacgtat
     8521 taacagtctt ggaaattatg aatgaaatgg cgcatcgagg ttatcgaatg caaccgatta
     8581 gtttagaaaa gagtcaggcg ttcgaattta tcattgaagg cgatacactt attccgccgt
     8641 tcatatcagt gcctgggctt ggcgaaaacg ttgcgaaacg aattgttgaa gctcgtgacg
     8701 atggcccatt tttatcaaaa gaagatttaa acaaaaaagc tggattatct cagaaaatta
     8761 ttgagtattt agatgagtta ggctcattac cgaatttacc agataaagct caactttcga
     8821 tatttgatat gtaaaatgaa ataatcaagg tatttattta atgcgtatgg cgtagtcaaa
     8881 gaaatacaaa attgttgctg gacacaaaat tatgcccgta tttcttttca atgtcttacg
     8941 agtctattca aatgtaatgg tgaaataaag gaacaaactt ttacaagaat ctctgattaa
     9001 tagtgaagtc atttgtttca agcataaact tatgctataa ttaagttgct taaaaattag
     9061 tgaactcagg cagaagagtg ggagattccc gctcttttct atttgccaaa aagggaggcc
     9121 tgtatgagta aaattacaga acaagtagaa gtgattgtta aaccaattat ggaagacttg
     9181 aattttgaac ttgtagacgt tgaatatgtc aaagagggta gagatcattt tcttagaatc
     9241 tctattgata aagaaggtgg cgtagattta aatgattgta cgctagcttc tgaaaaaata
     9301 agtgaagcta tggatgcaaa tgatcctatt cctgaaatgt attatttaga cgtagcgtca
     9361 cctggtgcag aacgtccaat taaaaaagaa caagatttcc aaaatgcaat aactaaacct
     9421 gtatttgttt ctttatatgt accaattgaa ggtgaaaagg aatggttagg cattttacaa
     9481 gaagtcaata atgaaacaat tgtagtacaa gttaaaatca aagcaagaac gaaagatata
     9541 gagataccga gagacaaaat agcaaaagca cgtcacgcag ttatgattta acgtgatgag
     9601 gaggaaaaaa cgtgtcaagt aatgaattat tattagctac tgagtattta gaaaaagaaa
     9661 agaagattcc tagagcagta ttaattgatg ctattgaagc agctttaatt actgcataca
     9721 aaaagaatta tgatagtgca agaaatgtcc gtgtggaatt aaatatggat caaggtactt
     9781 tcaaagttat cgctcgtaaa gatgttgttg aagaagtatt tgacgacaga gatgaagtgg
     9841 atttaagtac agcgcttgtt aaaaaccctg catatgaaat tggtgatata tacgaagaag
     9901 atgtaacacc taaagatttt ggtcgtgtag gtgctcaagc agcgaaacaa gcagtaatgc
     9961 aacgtcttcg tgatgctgaa cgtgaaattt tatttgaaga atttatagac aaagaagaag
    10021 acatacttac tggaattatt gaccgtgttg accatcgtta tgtatatgtg aatttaggtc
    10081 gtatcgaagc tgttttatct gaagcagaaa gaagtcctaa cgaaaaatat attcctaacg
    10141 aacgtatcaa agtatatgtt aacaaagtgg aacaaacgac aaaaggtcct caaatctatg
    10201 tttctcgtag ccatccaggt ttattaaaac gtttatttga acaagaagtt ccagaaattt
    10261 acgatggtac tgtaattgtt aaatcagtag cacgtgaagc tggcgatcgc tctaaaatta
    10321 gtgtcttctc tgaaaacaat gatatagatg ctgttggtgc atgtgttggt gctaaaggcg
    10381 cacgtgttga agctgttgtt gaagagctag gtggtgaaaa aatcgacatc gttcaatgga
    10441 atgaagatcc aaaagtattt gtaaaaaatg ctttaagccc ttctcaagtt ttagaagtta
    10501 ttgttgatga aacaaatcaa tctacagtag ttgttgttcc tgattatcaa ttgtcattag
    10561 cgattggtaa aagaggacaa aacgcacgtc tagctgctaa attaaccggc tggaaaattg
    10621 atattaaatc agaaacagat gcgcgtgaag cgggtatcta tccagtagtt gaagctgaaa
    10681 aagtaactga agaagatgtt gctttagaag atgctgacac aacagaatca accgaagagg
    10741 taaatgatgt ttcagttgaa acaaatgtag agaaagaatc tgaataatag gttggagtga
    10801 agtatctatg aaaaagaaaa aaattccgat gcgaaaatgt attctttcaa atgaaatgca
    10861 tcccaaaaaa gatatgattc gtgttgttgt taataaagaa ggcgaaatct ttgcggatgt
    10921 tactggaaag aaacaaggcc gtggcgcata tgtttctaaa gatgttgcta tggttgaaaa
    10981 agcacaacaa aaagaaattt tagaaaaata ttttaaagca tctaaagagc aattggatcc
    11041 tgtttacaaa gaaattatta gattaattta tagagaagag atcccaaaat gagtatagat
    11101 caaatattaa actttttagg attagcaatg agagctggta aagtaaaaac aggtgaatca
    11161 gtcattgtta atgagattaa aaaaggaaat ttgaagctcg ttattgttgc aaatgatgcg
    11221 tctgataata cagctaaatt aattacagat aaatgtaaga gttacaaagt tccattcaga
    11281 aagtttggaa atcgaaatga attgggaata gcacttggaa aaggtgagcg tgttaatgta
    11341 gggattactg acccaggctt tgctaaaaag ttgctatcaa tgatagatga atatcataag
    11401 gagtgattat atgagtaaac aaagaattta cgaatatgcg aaagaattaa atctaaagag
    11461 taaagagatt atagatgagt taaaaagcat gaatattgag gtttcaaatc atatgcaagc
    11521 tttggaagat gaccaaatta aagcattaga taaaaagttc aaaaaagaac aaaagaacga
    11581 caataaacaa agcactcaaa ataatcacca aaaatcaaac aatcaaaacc aaaataaagg
    11641 gcaacaaaaa gataacaaaa agaatcaaca acaaaataat aaaggcaaca aaggcaataa
    11701 aaagaataat agaaataata agaaaaataa caagaataat aaaccacaaa atcaaccagc
    11761 tgctccaaaa gaaataccat caaaagtgac atatcaagaa ggtattacag taggcgaatt
    11821 tgcggataaa ttaaatgttg aatcatcaga aattatcaaa aaattattct tacttggtat
    11881 tgttgctaat atcaatcaat cattaaatca agaaacaatc gaattaattg ccgatgatta
    11941 tggcgttgag gttgaagaag aagttgtgat taatgaagaa gacttatcaa tctatttcga
    12001 agacgaaaaa gatgatccag aggcaattga gagaccagca gttgtaacaa ttatgggaca
    12061 tgttgaccat ggtaaaacga ctttattaga ttcaattcgt catacaaaag ttacagcagg
    12121 tgaagcaggc ggaatcactc aacatattgg tgcatatcaa attgaaaacg atggcaaaaa
    12181 aatcactttc ttagatacac cgggacatgc tgcatttaca acgatgcgtg cgcgtggtgc
    12241 acaagtaaca gatattacta ttttagtagt agcagctgac gatggtgtta tgccacaaac
    12301 aattgaagca attaaccatg ctaaagaagc agaagtacca attattgttg cagtaaataa
    12361 aattgataaa ccaacttcaa atcctgatcg agttatgcaa gaattaactg aatatggttt
    12421 aattcctgaa gattggggcg gcgaaacaat tttcgttcca ctttctgcat taagtggtga
    12481 tggtatcgac gatttattag aaatgatagg attagttgca gaagttcaag aacttaaagc
    12541 aaatcctaaa aaccgtgctg ttggtacagt tatcgaagct gaattagata aatcacgtgg
    12601 tccttctgca tcattattag tacaaaacgg tacattaaat gttggtgatg cgattgtagt
    12661 tggtaatact tacggccgta tccgtgcaat ggttaatgac ttaggtcaaa gaatcaaaac
    12721 ggctggtcca tcaacgcctg ttgaaattac aggtattaat gatgtgccac aagctgggga
    12781 tcgctttgtt gtatttagtg atgaaaaaca agctcgtcgt attggtgaat caagacacga
    12841 agctagcatt atacaacaac gtcaagaaag taaaaatgtt tcattagata acctgtttga
    12901 acaaatgaaa caaggtgaaa tgaaagattt aaacgttatt attaaaggtg atgttcaagg
    12961 ttctgttgaa gctttagctg catcattaat gaaaattgat gttgaaggcg taaatgttcg
    13021 tatcattcat acagcggttg gtgcaattaa tgagtcagac gtgacacttg ctaatgcctc
    13081 aaatggtatt atcattggtt tcaatgttcg tccagacagt ggtgcaaaac gtgctgcaga
    13141 agctgaaaat gttgatatgc gtttacacag agttatttat aatgttatcg aagaaattga
    13201 atcagcgatg aaaggtttac ttgatccaga atttgaagaa caagttatcg gacaagctga
    13261 agttcgtcaa acattcaaag tttctaaagt tggtactatt gctggatgtt atgttactga
    13321 aggtaaaatt acgcgaaatg ctggtgtacg tattattcgt gatggtattg ttcaatatga
    13381 aggcgaatta gatacactta aacgtttcaa agatgatgct aaggaagttg caaaaggtta
    13441 tgaatgtggt attacaattg aaaactacaa tgaccttaaa gaaggcgatg ttatcgaagc
    13501 atttgaaatg gttgaaatta agcgttaatt aaataaatta caagctaaaa gtatagttaa
    13561 gattgatatg ctccctataa atattgcact ttttaagtgt ctactttata gggagcatat
    13621 ttgatactag cttttggttt tttattagaa tagattacct attaaaagtt acgttatatg
    13681 gacatgattt tgtataaaat tttgtggtgg cctagaatga tttttaatga caaaatataa
    13741 tgtcgactat tattggaaaa ttttctgttg aaatgcctat cttacggcaa actttatttg
    13801 attttatagg cttaatttat taaaataacg tgtgagctaa aataattgtt taagcattgt
    13861 tacactaaaa aatgcaaata acaattgaac ttaaagataa agaggtgaca agaatgagca
    13921 gtatgagagc agagcgtgtt ggtgaacaaa tgaagaagga attaatggat atcatcaaca
    13981 ataaagtcaa agatcctcga gttggtttta ttacaattac agatgttgtt ttaacaaatg
    14041 atttatcgca ggctaaagta tttttaactg tattaggtaa cgataaagaa gtagaaaata
    14101 catttaaagc acttgataaa gcaaaaggct tcattaagtc tgaattaggt tctagaatgc
    14161 gattacgtat tatgccggaa ttaatgtatg aatatgatca atcaatcgaa tatggtaata
    14221 aaattgaacg aatgattcaa gatttacaca aacaagatag ataatttagt gttaggtatc
    14281 tggaaaatgt ttgataattt cttaatatcg gtatattaac attaaacagt taatacatag
    14341 atgtgtagaa atagttaaca ttttccagtt tttttatgaa taaatttagt tgatacgcta
    14401 ttaaaatata ttttaaaaaa gaaggtgact atatgtataa tgggatatta ccagtatata
    14461 aagagcgcgg tttaacaagt catgacgttg tattcaaatt gcgtaaaata ttaaaaacta
    14521 aaaaaatagg tcacacgggt acgcttgatc ccgaagttgc aggcgtgtta ccggtatgta
    14581 taggtaatgc aacgagagtt agtgattatg ttatggatat gggcaaagct tatgaagcaa
    14641 ctgtatcgat aggaagaagt acaacgactg aagatcaaac gggtgataca ttggaaacaa
    14701 aaggtgtaca ctcagcagat tttaataagg acgatattga ccgattgtta gaaagtttta
    14761 aaggtatcat tgaacaaatt ccgccgatgt actcatccgt caaagtaaat ggtaaaaaat
    14821 tatatgaata tgcgcgtaat aatgaaacag ttgaaagacc aaagcgtaaa gttaatatta
    14881 aagacattgg gcgtatatct gaattagatt ttaaagaaaa tgagtgtcat tttaaaatac
    14941 gcgtcatctg tggtaaaggt acatatatta gaacgctagc aactgatatt ggtgtgaaat
    15001 taggctttcc ggcacatatg tcgaaattaa cacgaatcga gtctggtgga tttgtgttga
    15061 aagatagcct tacattagaa caaataaaag aacttcatga gcaggattca ttgcaaaata
    15121 aattgtttcc tttagaatat ggattaaagg gtttgccaag cattaaaatt aaagattcgc
    15181 acataaaaaa acgtatttta aatgggcaga aatttaataa aaatgaattt gataacaaaa
    15241 ttaaagacca aattgtattt attgatgatg attcag
//
LOCUS       DANJFZ010000061        15036 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.061, whole genome shotgun sequence.
ACCESSION   DANJFZ010000061 DANJFZ010000000
VERSION     DANJFZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.061"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            95..373
                     /locus_tag="PWN45_001644"
     CDS             95..373
                     /locus_tag="PWN45_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDL0623413.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            388..756
                     /locus_tag="PWN45_001645"
     CDS             388..756
                     /locus_tag="PWN45_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623414.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            744..1040
                     /locus_tag="PWN45_001646"
     CDS             744..1040
                     /locus_tag="PWN45_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDL0623415.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            1374..2480
                     /locus_tag="PWN45_001647"
     CDS             1374..2480
                     /locus_tag="PWN45_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDL0623416.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            2474..3814
                     /locus_tag="PWN45_001648"
     CDS             2474..3814
                     /locus_tag="PWN45_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDL0623417.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            3912..4802
                     /locus_tag="PWN45_001649"
     CDS             3912..4802
                     /locus_tag="PWN45_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0623418.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(4949..5779)
                     /locus_tag="PWN45_001650"
     CDS             complement(4949..5779)
                     /locus_tag="PWN45_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623419.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(5799..6809)
                     /locus_tag="PWN45_001651"
     CDS             complement(5799..6809)
                     /locus_tag="PWN45_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0623420.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(6907..7611)
                     /locus_tag="PWN45_001652"
     CDS             complement(6907..7611)
                     /locus_tag="PWN45_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0623421.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(7604..8161)
                     /locus_tag="PWN45_001653"
     CDS             complement(7604..8161)
                     /locus_tag="PWN45_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0623422.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            8292..8591
                     /locus_tag="PWN45_001654"
     CDS             8292..8591
                     /locus_tag="PWN45_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDL0623423.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            8611..9432
                     /locus_tag="PWN45_001655"
     CDS             8611..9432
                     /locus_tag="PWN45_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDL0623424.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            9449..9826
                     /locus_tag="PWN45_001656"
     CDS             9449..9826
                     /locus_tag="PWN45_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0623425.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(9976..10206)
                     /locus_tag="PWN45_001657"
     CDS             complement(9976..10206)
                     /locus_tag="PWN45_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDL0623426.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(10426..10926)
                     /locus_tag="PWN45_001658"
     CDS             complement(10426..10926)
                     /locus_tag="PWN45_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623427.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(11124..11693)
                     /locus_tag="PWN45_001659"
     CDS             complement(11124..11693)
                     /locus_tag="PWN45_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0623428.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            11848..11976
                     /locus_tag="PWN45_001660"
     CDS             11848..11976
                     /locus_tag="PWN45_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623429.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            12103..13158
                     /locus_tag="PWN45_001661"
     CDS             12103..13158
                     /locus_tag="PWN45_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDL0623430.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            13766..14467
                     /gene="isaA"
                     /locus_tag="PWN45_001662"
     CDS             13766..14467
                     /gene="isaA"
                     /locus_tag="PWN45_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDL0623431.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            14743..>15036
                     /locus_tag="PWN45_001663"
     CDS             14743..>15036
                     /locus_tag="PWN45_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623432.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKL"
BASE COUNT         5047 a         2339 c         2739 g         4911 t
ORIGIN      
        1 taaaacaata tttaagtata attgaatgtt tgaatatcat atattgatac agtttctaat
       61 aattttaaaa taatttaaat ggagagaggt gtaaatgatg agtacagttc aaagtgatat
      121 ttttaagacc aatagtgcat catcatctat taaaagcgct gttgaaacat gtaataatgt
      181 gtcgaaaccg gataaagatg aaagtacaac agtaagtgga aataataatg ctcatagtgt
      241 gatagatgat ttgatgagta agaatcaatc tgttgctgaa gcaatacgaa ctgcgagcga
      301 taatatacaa aaagttggtg aggcttttga ccaaactgac gtaatgattg gtaatgaaat
      361 tggtaaaaat taaaacgtgg tgaaatgatg tcgaataaac tggatgaaat caataaaata
      421 atcacagcga aacatgagca aatggatgac ttatatgatg aaaagcgaga ggttaaagca
      481 ttgatagatg aaagtgatgc gcttaatcat tcgatagatc aattatatca acatttaggt
      541 gagcgttatt atagtagcaa tatggctagt cgtatggaac agttccgcga tgaatttcat
      601 tttgcgaaac gacgttcaac ggaagcgtta tacgagcagc aacagcaaat tcaacatggc
      661 attcgtaaag tggaagaaga gatgattgac ttggaaatgc gaaggaatgt tgaaattgag
      721 acggtgacaa aggaggaaaa taaatggaaa caataggaag cattatttat ttaaaagaag
      781 gttcgcaaaa gttaatgatt attaatagag gaccaattgt agaaattgaa aatcaaaagt
      841 atatgtttga ctattctgca tgtaaatatc cgattggtgt tgtagaagat gaaatttatt
      901 attttaacga ggaaaatata gattcagtta tttttaaagg ttattctgat caagatgagg
      961 ttagatttca agagttgttt gaaaatatga aacaaaattt ggatagtgaa atacaacgtg
     1021 gagaagttac acaacaataa agaaatactt tttctttatt ggggtgggac gacgaaataa
     1081 attttgtaaa aatatgattt ctgtctcacc gccaaaatgt tatctccaag tactgaggct
     1141 ggatgatacc atgaaatgtc attcataatc atttgaacta aacgtagcag ccttaaattt
     1201 taaaaaaaga cacataccaa cttccgaaat gtagatgaat tctctacaat aacggaagtt
     1261 tttcttttaa tattgaaatt tctcaaggat aggtctatac tttataaatc gtaattatta
     1321 cgatttataa tcaaaaacaa taacttgaaa tagatcattg agggagtgtt aatatgcaac
     1381 atcataaagt ggctattatc ggtgccggtg ctgcaggtat aggtatggcc attaccttaa
     1441 aagatttcgg tataacagat gtcattattt tagaaaaagg aacagtagga cattcattta
     1501 aacattggcc gaaatcgacc cgtacgatca cgccatcatt tacgtctaat ggatttggca
     1561 tgcctgatat gaatgcaatt tccatggata cttcaccagc atttacattt aatgaagaac
     1621 atatttccgg agaaacatat gctgaatatt tacaagtggt tgccaaccat tacgagctga
     1681 atatctttga aaatacagtt gtcacaaata tatctgtaga tgatgcatat tatacgattg
     1741 caacgacaac agagatatat cacgcggatt atatctttgt cgcaacaggt gattataatt
     1801 tccctaaaaa gccatttaaa tatggtattc attatagtga aattgaagac tttgataact
     1861 ttaataaggg gcaatatgtg gttatcggag gtaatgaaag tggctttgat gctgcatatc
     1921 aacttgcaaa aaatggctct gacatcgcac tttatactag cacaaccggt ttaaatgatc
     1981 cggatgctga tcctagtgtt agattgtcac cttatacacg tcagcgacta ggtaatgtca
     2041 ttaagcaagg tgctcgcatc gaaatgaatg tacattatac agttaaagat attgatttta
     2101 acaatggaca gtatcatatc agttttgata gcggacaaag tgtgcttaca cctcatgaac
     2161 caatactagc aactggcttt gatgcaacaa aaaatccaat cgttcaacaa ttatttgtga
     2221 caacaaatca agatattaaa ttaacaacac atgatgaatc gacacgttat ccgaatattt
     2281 ttatgattgg tgcaacagtt gaaaatgata atgccaaatt atgctatatc tataaattta
     2341 gagcgcgatt tgcagtactt gcacatcttt taacacagcg ggaaggctta ccagctaaac
     2401 aagatgtcat tgaaaattat caaaaaaatc aaatgtattt agatgattat tcatgttgtg
     2461 aagtgtcatg cacatgttag aagtgaaata tgatatgaga actgggcatt atacgcccat
     2521 acctaatgaa cctcattatt tggttattag tcatgcggat aaacttaccg caacagaaaa
     2581 agcgaaatta agattattaa tcataaaaca gaaattagat atttcattgg cagaaagtgt
     2641 agtttcttcg cctatagcga gtgaacatgt gatagaacaa ttgacactat ttcaacatga
     2701 gcgacgacat ttaagaccta aaataagtgc gacattttta gcctggttgt tgatattttt
     2761 aatgtttgca ttgccaatcg gtatcgctta tcaattttca gattggtttc aaaatcagta
     2821 tgtgtcagca tggatagaat atttaactca aacaacattg ctcaatcacg atatattaca
     2881 gcatatatta tttggtgatt atggtgtgct atcacttgga acatattcgc tcgtatgggc
     2941 attgccggtt gtaatattga ttagtttatc aactgctata attgatcaaa caggactcaa
     3001 atcatggatg atatgggcaa ttgaaccgtc aatgttatgg ataggattac aaggtaatga
     3061 tatcgtgcca ctattagaag ggtttggatg taatgcagca gctatttcac aagcagcaca
     3121 ccaatgccat acctgcacga agacacagtg tatgagttta ataagctttg gtagttcttg
     3181 tagttatcaa ataggtgcga cattatctat ttttagtgta gctggaaagt catggctatt
     3241 tatgccgtac ttaatattag tacttttagg tggcatctta cataatagga tatggttgaa
     3301 aaagaatgat caacaactta gcgttccgct accttatgat aggcaattac atatgccaaa
     3361 tatacgtcaa atgttgctac aaatgtggca aaatatacaa atgtttatcg ttcaagcgct
     3421 acctattttt atcacaatct gtcttattgt tagtatttta tcactaacgc caattttgaa
     3481 tgttttatca caaatattta cacctatatt atcgttatta ggcatctcgt cagaattgtc
     3541 accagggatt ttattttcaa tgattcgaaa agacggcatg ctcttgttta atttgcatca
     3601 gggcgcctta ttacaaggaa tgacagcaac acagttacta ctacttgtgt tttttagttc
     3661 aacatttaca gcgtgctcgg tcacaatgac gatgcttttg aaacatttag gtggtcagtc
     3721 agcactaaaa ttaattggaa agcaaatggt gacatcattg tctttagtta ttggtgtagg
     3781 catcattgtt aaaatagtaa tgctgattat ttaaaaaaaa tgaactataa ctgaatatag
     3841 agtcatgtca gtcaatagga gatctatctt ggaatatgct attcatatga agtataagag
     3901 gagagtcgca gatgaaaata gttattatag gtgggttttt aggtggcggt aaaacgactg
     3961 tcttaaatca tttgctcgct gaatcattaa aggaatcgct gaaaccagca gtcatcatga
     4021 atgaatttgg gaaaatgagt gttgatggtg ccttagtatc tgaagacata cctttaagtg
     4081 aactgacaga ggggtgtatc tgttgtgcaa tgaaagcaga tgtatcagaa cagttacatc
     4141 aattatattt aaaagagcaa ccagacattg tatttattga atgtagtggg attgcagaac
     4201 cggtctctgt cttagatgct tgtttaacgc ctattttagc tccgtttaca acaattacac
     4261 atatgattgg tgtaatagac gcaagcatgt ataaacacat taaatcattc cctaaagaca
     4321 tccaaggctt attttatgag caattagcat attgttctgt cttatttgtt aataaaatag
     4381 attcagcaga tgttgaaaca acgagcaaac tattgaaaga tttagaagtt attaacccag
     4441 aggccgatat acaagtcggt atgcatggca gcgtcacttt gccaatatca gttagacaaa
     4501 tgacagcaac ttctgacaat aaacataagt ctttacatca aatgattaat catcaatttg
     4561 tgcaatcacc agtcaaatgt actaaagcag agtttataaa acgtttagca tgccttccgt
     4621 ctcatattta taggttgaaa gggtttatga catttgaaga caccgcacat acgtatctca
     4681 ttcaatttac acaaggacaa tatgaattaa cacctgtagc attttcaaaa aaagtgccag
     4741 aatatttggt gcttatcgga aagggtattt caaaagaaga ctatcaatgt ttggaacagt
     4801 agtgttttca gtggaagaga atggttaaca tgccttcatg tataataacg agttgatttg
     4861 aacgtttaag cgtaaataaa aataagcttg gtcagccatc aaatataatt tgaaaactgt
     4921 ccaagctgtt ttattagaga acaatcaatt aaccccacat atttaataat acatcagcaa
     4981 agccttcagg tttttgaata taacctaagt gaccgcctgg aatatctaca ataggtatgc
     5041 cagtttcttt atttatataa aagttaacat cttgtgggaa ggagcctcta gaatctgtcc
     5101 catttagtag ggtgatttta tcgctgtatt ttgtgaaatc atccaaagta atatttgaat
     5161 gcgtatattg tctaatttca aattctaacc agaacatcgt acgtttgtac tgttctatac
     5221 gtccttcttc agtatcagca ggttgagaca tcatttttgc atcaattggt gcgatattta
     5281 atgtttcgcc aaatgttttc atgccttttt ctaagccttc tgttaaaatt tgatgcacaa
     5341 tgtcatcatt tttatctttc caataagtac tgtctggtaa aaatgtatta attggtggtt
     5401 cgtgaaatgc aatcttttta acgacttcag ggtaatcttt taacacatgc atcgcaacga
     5461 ttgaacctga acttgaacct aatatataga caggttcatc acttaatgac tttgcaagtt
     5521 cggcaatgtc ctgtgcgtcg cgtttgacac gataatcact gtcagggttt gaagcggaat
     5581 cagggagtgg ttcagttaac tcgctttctc cataatcacg acgatcaacg gctacaacag
     5641 taaaatggtc ttttaactgt tctgcaagag gcagaaaaat gtctccggta ccgtttgcac
     5701 caggaataaa gatgagcacg ggtccttgtc cgacttggtg gtatcgtaat ttagcgcctt
     5761 gtaattctaa agtttccata ttcaatgacc tccatttgtt aattgttagg tgataaacct
     5821 aataatttag caccatttgt ataacttatt ttctcttttt cttcatctgt taaacccagt
     5881 tcatctaaaa atacacctaa tttttcaggc tcaatatatg gataatcagc agcataaaga
     5941 attctatcaa tacctacttc tttcttgact aaatcaaact gtggcttcgt taacatgcca
     6001 ctcggtgtga tataaaaatt atttttaaag taatagctta cagggtggtt caaatgttca
     6061 gcgaataaag cttcatccat acgttctaag aagaatggga taaactcacc ccaatgtcca
     6121 ataatcatat ttaactttgg ataacgatca aaaataccag ataatactag atgtattgca
     6181 tgaatgccga catcaatgtg ccaaccataa ccaaaacaag caaatgttgc cgcagttact
     6241 tcaggataat ttcctttata gtatgattga taaatgtcac tgttaactgg cgcgggatgt
     6301 agataaatcg gtacgtctaa attttcagct gttttgaaaa taatgtcata tttgtcttga
     6361 tcaagaaaac catcttgtgc acgtcccata atgagcgcac ctttgaatcc taaatcattg
     6421 atgcaacgtt cgaattctcg cgctgcggct tcaggctcat tgataggtaa agttgcaaag
     6481 cctacaaagc gattgggata ttgtgcaata tagtttgcca attgatcatt tgctttttga
     6541 cataattcaa tggctttctg accaaccaaa tttgaaggag aaccatttcc ataagataag
     6601 acttgaattt gaacgtcttg attattcata aattggatac gttcatcatg atgtgataat
     6661 tcgtcggcat ttgtaaaacc tgtctttttt tcaaggcctt ctaacattac tttcatcggt
     6721 acacctttag gatctgctga tatcgcattc atcgtttctt tttgaatatc ttcaatgaca
     6781 taatgttctt caaacgtaat acttttcatt tacttcgcct ccatattgta ttgcatgttt
     6841 attgcatcta ttgcagaagc attttttata tacctctaat ttcaatgttt gtaacataaa
     6901 attgatctac caaggcatct ctccatcgcc attaataaat gtacctgttg ggccatctgc
     6961 accaatcgtt gctaattgaa tgattggctt gattccttca gaaacgtgtt tggaattatt
     7021 actaaaatca ccaactaaat cagtatttgt agcgcctgga tcagcagcat tgatttgcat
     7081 gttaggtaat cctttagcgt attgtagcgt tagcattgtt actgccgatt tagacgaaca
     7141 ataagctaat gaattcactt tagattcagc tgtttcgggg tttgtaacca ttccaaatga
     7201 acctaaacca cttgatacgt tgacgacaac aggttgttca gatttttcta agagagggac
     7261 gaatgtattc atcattcgta cgataccgaa tacattcgtt tgatatactt cttcaacgtc
     7321 acgaggtgtc aatttggaag gtgctgaaaa ttgaccagat atacctgcat tgttaatgag
     7381 gatatcaaga cggccttctt tttcagcaat catgttataa gcatttttga ctgagtagtc
     7441 acttgtaaca tctaattgta cataatgaac acctaatttt tgtgatgctt gttgtcctct
     7501 tacatcattc cgagaaccta tataaacttt gtaacccaat gctttaagtg cctctgcact
     7561 tgcatagcct aaccctttat tgcctcctgt gattaacaca attttagtca ttacgtccca
     7621 cctcatctaa ataaatgttt aataaataat ttctgtacgc ttcaattgaa atatggcgat
     7681 gctctatttg gaaggcaaat acactagttg ataatgattg caacagcata tctgttttga
     7741 acttcgtgta agtgtcgtca tcgcttttaa ataagtcata ataaaaatca aataattctt
     7801 gataaaatgc gctttggtaa aaacgtaatt tattgttgcc tgcttcaata cattgcagta
     7861 gtgccttatt atcgatttta aattgtaaaa gataatctaa cgacacttgc ataacctcat
     7921 aattagaatg atagtcatct ttaatttgct taaaatgagt gataaaaata tcaaggtctc
     7981 tttgtatgac gtagtagcat aaatcgcttt tatctttgaa atgtcgatac aatgtcccca
     8041 taccgatacc tagttcttta gcaatacgat tcatactaat gttttcaacg ccttcttcat
     8101 caaaaagttt gtgcgctatt tcttcaattc gttgcctatt ctcttttgca tcttttcgca
     8161 tgattacacc tacttaaaat tctctaaaat tgacaaacgg ataactctcc gtttattata
     8221 aaacgtgtta agaaagttag caatgaattt gcaataacta ttaaatatca taaaagaaaa
     8281 gagtgttgat aatgtcttta aataaagagc aaagacgcat cacagctgaa gagttgcaag
     8341 cacatttcga agcatctacc ttatcggttc aaatgattgc tgaaaaactg aatgtcacta
     8401 cagaagatgt ggaaaaagta ttagctatga cagcgccact aggcattttt agtcatcaat
     8461 tacaacgatt tattcattta gtatgggatg tcagagatgt aataaacgac aatattaaag
     8521 gaaatggaca aacaccagaa ccatatacgt atttaaaagg tgaaaaagag gactattggt
     8581 ttttaagata aaaagaggag tggatctaaa atgaaagata ttttagtaat aggcgcaaca
     8641 gggaagcaag gtaatgcagt tgtaaaacag ttgttagagg atggatggta tgtaagtgca
     8701 ttaactagaa ataagaataa tcgtaaactt tcagacatag gacatccgca tttatcaatc
     8761 gttgaaggtg atctgagtga taatgttagt ttgcaatcag cgatgaaagg taagtatggc
     8821 ttatacagta ttcagccaat cgttaaagat gatgttagcg aagaattaag acaaggtatg
     8881 aagataattg aaatagctga gcaagaaaat attcaacata ttgtgtatag tactgcggga
     8941 ggggttaatc gaaatcgaac tggcccacac tttgaagtac tagcaaaaat agaaaataga
     9001 ttaatggaat ctaatataaa tgcaacagtt atcaaaccat cattctttat ggataatttt
     9061 ttacgcattg ctaaagtcga agatgaacgt attacattac cagaatttat taatccgaat
     9121 ataaaattca caatgatttc ttctattgat atagctaaaa ttgcatcgta tatttttgca
     9181 catccacaaa gctttacgca tcaatcaata gagattggtt ccgatgaagt tacattaagt
     9241 gaagcagcaa caatttttag tgaagtgaca ggtaaatcta ctgttataga aggagaattt
     9301 gttagtggtg ttgcagaaaa gcaatggttg gaagaaaaag gttatgaagt agactttgaa
     9361 ttaatggctg aaataaatcc aacaagatta tcattaagtg attggcttaa agtccaaaac
     9421 tataataagt aatgaatggg gtgcaatcat gattcaatca atgtggttta atttgcatgt
     9481 gcaagattta gaaaagagcg cacagtttta taaagcgtta ggatttaaaa taaatagaaa
     9541 cccacaaatg ttagataaaa tggtcggcat tcaaataggt caaacaaccg taattttaat
     9601 agaaaacaag cattttcaaa atgtaagtca gcaaagcctt aatactgaac caaatgaagt
     9661 gatgatttct ctaggtgtga acacaaatga agaagttgac cagttggtga ataaagtgaa
     9721 agaagcgggt ggcacagtcg ttcaagaacc aacagtaagc caagggtttt atggagcaat
     9781 gttcaaagat cttgatggac accattttaa ttttttggtc tgctaaaatg aaattctcaa
     9841 aagaataaat aaaaagccca tacagagatg aagcgtaatg tgcaccatgg tgcgacacat
     9901 tagtgtcttc aatcagtatg ggctttgttt atgaggtcag ttggcacctt aagtgcaata
     9961 cctcgtgagc aaatattaat tttgaatata accgtattgt tggcgaagat cataaacttt
    10021 atctggtgtg acatcctgac cgattggatc aaccactttt attgtggaaa atgttgttaa
    10081 tacttgtcct cggatcatgc ttaagtagtc ttgacgcaat gcatgttgtt cttgtttttc
    10141 agctacagta agtggttgta ctttttcttt atttgcaagt tcattaattc tatctaaaat
    10201 tttcatattg tcatatcctc cttagaatca agtgttgata tattttgaga ttatttttat
    10261 atggtaattt atcaacacat taagtaatgt ttttagtttc tcggtctttt gaccagcaga
    10321 tttttggaat taaaataagg gatttgagac acgattttac tagtagtacg atggcaaagt
    10381 gatgttattg aagtgtcgtt tgatatacat gtctgcaatg ttgttttata agacaggatg
    10441 acgtaactga gcaaagaaat gagcattgtc gaatgacatc gtgtgtctta ttaaaattgc
    10501 aatgggcata gcataggtat attcacagtc gattttcttg atttctacat tatcataata
    10561 cgtagtgtaa aagttatacg acagtcgata gtcgattcgc tttttaccac gagcaattat
    10621 tatgtgttgc ttcaacgtcg actttaattt atttgtaatg tatgtcacta cacccagact
    10681 actctgatta atatttaata tattatgcgt gcgttcaaac ccctgaggtt catctgtctc
    10741 gctcacgtca atccctccta tcttagtatt taaacaacgc atatcaacta tgcaacatat
    10801 gttgtttaga catactatac tactatgtcg tacgtttgcc aataaacacg cacatatcat
    10861 ttgttgccta aacaacatat tgcctgaagt gttgtttaag tacgcaaaaa caaaaatgct
    10921 tgtcactaaa atctatatgt tttaatgaca agcttatttt cattcagtta acgactgcta
    10981 atatgacatt gtagagtata ggacgttgat ttatgtccta gacacggcta atcaatcgta
    11041 atacgttaaa ttttattgtg tgtacaagta tgaacctaag tcacatagtg atttcaagat
    11101 gcatgactaa ataccaagtt ttattaaaaa tgtgctatat gatgaattaa aaatggaagg
    11161 ttgcgatcga taaacacatc ggctgtctct tgactcgtgt taaaccaatg taatgctaag
    11221 cctgagatac cagctgctaa aaaactcgca ctatttacaa tgatatctcg ttgatgctcc
    11281 ggatagctgt tttctaacat aatcgcaaaa atatcttcta attcattatt aatacgttga
    11341 tgtgctttgt tgcaaaatgc ttcactattt aattcgcaag actttgcagc atctttgata
    11401 taactcgcaa ttgaaatgaa aatattacgc agaacctttt cattaatgac attagaaatg
    11461 ctcaaattat cattcaagtc ttttaaaatg gttacagata atgtgtagtc taggaggtct
    11521 tctttatcag tgaaatgagc gtaaaatgtt gcacgattta gcgtagcaat atcagtgata
    11581 tctttaactg taatttgaga taatttcttt tctctagaaa ttttaagaaa agcatcgaca
    11641 agcaattgtt tggttctaac gattcttgga tctatggatt tactttccat catttgttca
    11701 ctacctaaca tctgttgttt taacacttat tatcagtatc ttctattata ttgtcaattg
    11761 tgttgatgat atgtaagtgc tgatacgctc ctaacaaata atgtaccttt ttggtaagca
    11821 tttgaatgtc tttcttctat tgtattaatg gattttaaaa atgaagataa aataaaagtg
    11881 tattgtaaca atatgtttga aaaattgcca atttctttat ctgaaaattc agaattttgt
    11941 gatatactat ttttcatagt cttgagttat agatgatact gctagcctaa atataacaac
    12001 atcggcatgc acatcttaga atttaagcta gtggcagctt ataacttaag gctctttatt
    12061 atttgcatac agatgtatat acataaatgg aaggatgata ttatgacatc aattaaaaca
    12121 attacaccca aagattttat atttagagtt ttatcaggtg tcgccatagg tattgttgcg
    12181 ggtttagttc ctaatgcgat acttggagaa atttttaaat actttatgga ttatcatccg
    12241 atttttaaaa cgttattagg cgttgtcgtt gcaattcaat ttacagtacc tgctttgatt
    12301 ggtgcattgg ttgccatgaa atttgaccta tcgccattag caattgctgt tgtggcaagt
    12361 gcagcatatg taggaagcgg tgcagcgcag tttaaaaatg gtgcttggat gattacgggt
    12421 attggtgatt taattaatac aatgattaca gcagcgattg cagtgttatt cattttactt
    12481 attcaacatc gcgtaggtag tatggcattg attgtattcc caacagttgt tggcggtatt
    12541 tcaggtgcaa ttggtgtttt aatattgccg tatacgaaaa tgattacaac agccatcggt
    12601 aatatggtta atggttttac agaattacaa ccgattgtca tgagtatttt gatttcaatg
    12661 gtatttagtc ttattattat ttcgccacta tcaactgtgg ccattgcctt tgcgataggt
    12721 attacaggtt tagcagctgg atcagcgtca attggtatct ccgcgactga agcagtattg
    12781 ataattggta caagtaaagt gaatcgttta ggggtgccgt tatccgtctt ctttggaggc
    12841 gttaaaatga tgatacctaa tatggtgaaa tatccaattc ttatgttacc aatattaacg
    12901 acagccattg tatcaggttt ggtatcagca ttagtaggta ttcatggaac gaaagagtca
    12961 gctggatttg gttttatagg aatggtagga ccgattaatg catttaaatt tatggaggtt
    13021 gattctgctt ggttgagtgt cttactaatt gtagtagcgt tctttgttgt accatttgta
    13081 acagcttggc ttgcagatat catttatcgt aaagtgtttc gtttatatac taatgatatt
    13141 tttaaattca tgggataata acagtatgtt ttttgaaaat atgagaccaa aaattattta
    13201 atgttattgt gaattgcgag aatgaagttg aacaatataa ttgagctgca gtttatttgc
    13261 gctaataaaa gataagacaa ttcacaaaag atgtgtgttt taaaactttg aatatacaac
    13321 aataatagca atcaatacat gtgtagaaac atatttgttg attgcttttt aattgcgttt
    13381 aatgtacgaa aataagagaa gttacatgtc agatatatta cagctatgta acaaaaatac
    13441 aatctgtaat attacgaaag ctgtaatagt tgagctaaat atagtaaaaa tcttatgtgt
    13501 taaagactat ttcaagggtt gattggattc taaagggcac atatttcaca ttacagtata
    13561 aaacagccat atttcaacat tacaaataac acttgatatt gtaatgtttt gtaaagaaag
    13621 tgtaatttac tggctggttt tttgtgatat agtgagtacc gttgaaagaa ataaaagaca
    13681 atttaaactt gcactacgac attcaaattc aaaatataat tactaaaaac ataaacttac
    13741 aattattact ggaggatttt ttactatgaa aaagacaatt atggcatcat cattagcagt
    13801 ggcattaggt gtaacaggtt acgcagcagg tacaggacat caagcacacg ctgctgaagt
    13861 aaacgttgat caagcacact tagttgactt agcgcataat caccaagatc aattaaatgc
    13921 agctccaatc aaagatggtg catatgacat ccactttgta aaagatggtt tccaatataa
    13981 cttcacttca aatggtacta catggtcatg gagctatgaa gcagctaatg gtcaaactgc
    14041 tggtttctca aacgttgcag gtgcagacta cactacttca tacaaccaag gttcaaatgt
    14101 acaatcagta agctacaatg cacaatcaag taactcaaac gttgaagctg tttcagctcc
    14161 aacttaccat aactacagca cttcaactac ttcaagttca gtgagattaa gcaatggtaa
    14221 tactgcaggt gctactggtt catcagcagc tcaaatcatg gctcaacgta ctggtgtttc
    14281 agcttctaca tgggctgcaa tcatcgctcg tgaatcaaat ggtcaagtaa atgcttacaa
    14341 cccatcaggt gcttcaggtt tattccaaac tatgccaggt tggggtccaa caaacactgt
    14401 tgaccaacaa atcaacgcag ctgttaaagc atacaaagca caaggtttag gtgcttgggg
    14461 attctaattc cttcataata aataaaagta atgtttagac attaaatata tagctcttcc
    14521 catagaaatt ggggagagtt tttttattaa ttaaaaatac attttttaaa aagtggatat
    14581 tgaagttagg gaaatttatt ggactttatt gtattactgc taaaatactt gtctttatta
    14641 agtgccaagt ggaatgaaac ttcatttgag gctgtactga gtacactaga tacaaactaa
    14701 acgcacattt tataattaaa ttaaatgaaa aaggagataa aaatggctat aaacataatt
    14761 gaatataata ggtcttataa ggaagagcta atcgagttta ttttatcgat tcaaaaaaat
    14821 gaatttaata taaaaattga tagagatgat caacctgatt tggagaacat cgaacataac
    14881 tatttgaata atggagggca attttggctt gcaataaaca atcatcaaaa tattgttggt
    14941 actataggtt tgattagact agataataat atgtctgcac ttaaaaaaat gtttgttgat
    15001 aaaggttata ggaatttaaa aataggtaaa aaactt
//
LOCUS       DANJFZ010000062        14766 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.062, whole genome shotgun sequence.
ACCESSION   DANJFZ010000062 DANJFZ010000000
VERSION     DANJFZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.062"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..897
                     /gene="lukS-PV"
                     /locus_tag="PWN45_001664"
     CDS             <1..897
                     /gene="lukS-PV"
                     /locus_tag="PWN45_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HDL0623433.1"
                     /translation="IITPIATSFHESKADNNIENIGDGAEVVKRTEDTSSDKWGVTQN
                     IQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPN
                     VDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEV
                     ERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYFVPDNELPPLVHS
                     GFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLEGSRIHNAFVN
                     RNYTVKYEVNWKTHEIKVKGHN"
     gene            893..1876
                     /gene="lukF-PV"
                     /locus_tag="PWN45_001665"
     CDS             893..1876
                     /gene="lukF-PV"
                     /locus_tag="PWN45_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HDL0623434.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2125..2310
                     /locus_tag="PWN45_001666"
     CDS             2125..2310
                     /locus_tag="PWN45_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623435.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2401..3318
                     /locus_tag="PWN45_001667"
     CDS             2401..3318
                     /locus_tag="PWN45_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDL0623436.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3375..4313
                     /locus_tag="PWN45_001668"
     CDS             3375..4313
                     /locus_tag="PWN45_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDL0623437.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            4306..4512
                     /locus_tag="PWN45_001669"
     CDS             4306..4512
                     /locus_tag="PWN45_001669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623438.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            4527..5441
                     /locus_tag="PWN45_001670"
     CDS             4527..5441
                     /locus_tag="PWN45_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDL0623439.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            5640..5759
                     /locus_tag="PWN45_001671"
     CDS             5640..5759
                     /locus_tag="PWN45_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623440.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      5842..5976
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6038..6583
                     /locus_tag="PWN45_001672"
     CDS             6038..6583
                     /locus_tag="PWN45_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0623441.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(6689..6937)
                     /locus_tag="PWN45_001673"
     CDS             complement(6689..6937)
                     /locus_tag="PWN45_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL0623442.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            7045..7998
                     /locus_tag="PWN45_001674"
     CDS             7045..7998
                     /locus_tag="PWN45_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0623443.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            7988..9367
                     /locus_tag="PWN45_001675"
     CDS             7988..9367
                     /locus_tag="PWN45_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL0623444.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            9520..10980
                     /gene="ebpS"
                     /locus_tag="PWN45_001676"
     CDS             9520..10980
                     /gene="ebpS"
                     /locus_tag="PWN45_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDL0623445.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            11105..11221
                     /locus_tag="PWN45_001677"
     CDS             11105..11221
                     /locus_tag="PWN45_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623446.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            11382..12368
                     /locus_tag="PWN45_001678"
     CDS             11382..12368
                     /locus_tag="PWN45_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDL0623447.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(12483..13451)
                     /locus_tag="PWN45_001679"
     CDS             complement(12483..13451)
                     /locus_tag="PWN45_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL0623448.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            13528..14187
                     /locus_tag="PWN45_001680"
     CDS             13528..14187
                     /locus_tag="PWN45_001680"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL0623449.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            14218..14334
                     /locus_tag="PWN45_001681"
     CDS             14218..14334
                     /locus_tag="PWN45_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623450.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            14451..14642
                     /locus_tag="PWN45_001682"
     CDS             14451..14642
                     /locus_tag="PWN45_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623451.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
BASE COUNT         5737 a         2132 c         2517 g         4380 t
ORIGIN      
        1 ataatcactc ctattgctac ttcgtttcat gaatctaaag ctgataacaa tattgagaat
       61 attggtgatg gcgctgaggt agtcaaaaga acagaagata caagtagcga taagtggggg
      121 gtcacacaaa atattcagtt tgattttgtt aaagataaaa agtataacaa agacgctttg
      181 attttaaaaa tgcaaggttt tatcaattca aagactactt attacaatta caaaaacaca
      241 gatcatataa aagcaatgag gtggcctttc caatacaata ttggtctcaa aacaaatgac
      301 cccaatgtag atttaataaa ttatctacct aaaaataaaa tagattcagt aaatgttagt
      361 caaacattag gttataacat aggtggtaat tttaatagtg gtccatcaac aggaggtaat
      421 ggttcattta attattcaaa aacaattagt tataatcaac aaaactatat cagtgaagta
      481 gaacgtcaaa attcaaaaag tgttcaatgg ggaataaaag ctaattcatt tatcacatca
      541 ttaggtaaaa tgtctggaca tgatccaaat ttatttgttg gatataaacc atatagtcaa
      601 aatccgagag actattttgt tccagacaat gaattacccc cattagtaca cagtggtttc
      661 aatccttcat ttattgcaac tgtttctcat gaaaaaggct caggagatac aagtgaattt
      721 gaaataacgt atggcagaaa tatggatgtt actcatgcta ctagaagaac aacacactat
      781 ggcaatagtt atttagaagg atctagaata cacaacgcat ttgtaaacag aaattacaca
      841 gttaaatatg aagtgaactg gaaaactcat gaaattaaag tgaaaggaca taattgatat
      901 gaaaaaaata gtcaaatcat cagttgttac atcaattgca ttgcttttgc tatccaatac
      961 agttgatgca gctcaacata tcacacctgt aagtgagaaa aaggttgatg ataaaattac
     1021 tttgtacaaa acaactgcaa catcagattc cgataagtta aaaatttctc agattttaac
     1081 ttttaatttt attaaagata aaagttatga taaagataca ttaatactca aagctgctgg
     1141 aaacatttat tctggctata caaagccaaa tccaaaagac actattagtt ctcaatttta
     1201 ttggggttct aagtacaaca tttcaattaa ttcagattct aatgactcag taaacgttgt
     1261 agattatgca cctaaaaatc aaaatgaaga atttcaagta caacaaacgg taggttattc
     1321 ttatggtgga gatattaata tctctaacgg cttatcaggt ggaggtaatg gttcaaaatc
     1381 tttttcagag acaattaact ataaacaaga aagctataga actagcttag ataaaagaac
     1441 taatttcaaa aaaattggtt gggatgttga agcacataaa attatgaata atggttgggg
     1501 accatatggc agagatagtt atcattcaac ttatggtaat gaaatgtttt taggctcaag
     1561 acaaagcaac ttaaatgctg gacaaaactt cttggaatat cacaaaatgc cagtgttatc
     1621 cagaggtaac ttcaatccag aatttattgg tgtcctatct cgaaaacaaa acgctgcaaa
     1681 aaaatcgaaa attactgtta cttatcaaag agaaatggat agatatacaa acttttggaa
     1741 tcaacttcac tggataggta ataattataa agatgaaaat agagcaactc atacatcaat
     1801 ttatgaagtt gattgggaaa atcatacagt taaattaata gatactcaat ctaaggaaaa
     1861 aaatcctatg agctaaacag atagataatc aaaaaatctt aaatatgtta aaatttacaa
     1921 acactttctt tctatattag ggtaaccacg tcttaattga cgtggttatt ttttcagggc
     1981 aaaaaaaggg cggattattt aaataaggac aaacacttgt ggaaaattta aaaggttaaa
     2041 aataataaag aacttggtat aacaagggtt ttatacattt gcgtacaacg acgaaatgtc
     2101 aatttaccat cacattatga cgatatgttt attttaaaca cacaagctca tgcgcgtctt
     2161 gatcaaatgg cacaacagtt tgaagttgtt tgtaatggct tgaacgaaaa tgaaggacaa
     2221 gcaattcaaa cgatggatca atctgcctct ctaatacggt caaacttaat tcaagttaaa
     2281 gaacaattag aaaaactagc tgtatactaa ttaatttatt aaatgctact tgttttcatt
     2341 gagaataagt agctttttca aacataaaag ttttacaaac acataaatgg gtgatgagct
     2401 atgtttaaaa gaactaaact aatcttaata gcaacattac tgctatcagg atgttcaact
     2461 accaataacg aatctaataa agaaacaaaa tcagtgccag aagaaatgga agcttcaaaa
     2521 tatgtaggtc aaggcttcca accacctgca gaaaaagatg tggttgaatt tgcgaaaaag
     2581 cataaagata aaattgccaa acgaggcgaa caatttttta tggataattt cggtctaaaa
     2641 gttaaagcta ccaatgttgt aggtagtgga aaaggcgtag aagtattcgt gcattgtgat
     2701 gaccacgata tcgtatttaa tgcgagtatt ccatttgata aatcaataat tgagagtgat
     2761 agctcattaa gaagtgagga caaaggcgat gatatgagta ctttagttgg tacagtgttg
     2821 agtggctttg aatatcgaac acaaaaagaa aagtatgaca atttatataa atttttcaaa
     2881 gataatgaag agaaatacca atatacaggc tttacaaaag aagcaataaa caagacgcaa
     2941 aatgtcggat ataaaaatga atatttttat atcacttact cgtcaagaag cttaaaagaa
     3001 tatcgtaagt attacgaacc attgattcat aaaaatgata aagaatttaa agaaggaatg
     3061 gaacaagcca gaaaagaagt gaattacgct gctaatacag atacagtaac aacattgttt
     3121 agtacaaagg aaaattttac taaagacaat acagttgatg atgtaattga actgagtgat
     3181 aaactatata attttaaaaa taagccagaa aaatctacaa ttacaattca aataggaaag
     3241 cctactatta atactaaaaa agccttttat gatgataatg atccaataga atatggagtg
     3301 tatcgtaaag atgaataaat ttttttaaaa tgctttttaa tattctatat aaacacataa
     3361 atgggtgatg agctatgttt aaaaaggcga aattaatctt aatagcaacg ctactgttat
     3421 caggatgttc agcgatgaat aatgaatcaa aaaaagacac aaatacagaa acaaatacag
     3481 aaacaaaatc agtaccagaa gaaatggaag cttcaaaata tgtaggacaa ggctttcaac
     3541 cacctgcaga aaaagatgcg attgaatttg tgaagaagca tcgtaaagaa tttgaaaaag
     3601 taggtgaaca attctttaaa gataactttg gactaaaagt taaagctaca aatgttgtag
     3661 gtaaagatga tggtgtagaa gtttatgtgc attgcgaaga tcatggcatt gtatttaatg
     3721 caagtctacc tttgtacaaa gatgccatcc atcaaaaagg atcaatgcgt agtaatgaca
     3781 acggtgatga tatgagtatg atggtgggta cagtgctgag tggctttgaa tatcgagcgc
     3841 aaaaagaaaa gtatgataat ttatataaat ttttaaaaga aaatgaaaag caatatcaat
     3901 atacaggttt tactaaagaa gcaattaaca agacgcaaaa cgtcgggtat caaaatgaat
     3961 atttttatat tacatattta tcaagaaatt taaaagaata tagaaaatat tacgaaccat
     4021 tgatacataa aaatgataaa gagtttaaag aaggtatgca acgagctaga aaagagctaa
     4081 actatactgc taatacaaat actgtagcaa cgttgtttag tacgaatgat gaaaggaata
     4141 gaaaagaaaa gataaataat gtaatagatt tatccgagaa aattgaaaga acaaaagata
     4201 tgccaatcaa gaatactata actactcaat taggaaataa acttattggc acaaaaaaag
     4261 ctcgttttga tgataagaaa gtagtgtcgt ttggagcatt tgaagatgaa taaaataaat
     4321 gatagagatt taacagaatt gagtagctat agggtttatc aagacatcaa taaagataat
     4381 gactttacag ttaacgaaaa acgatttaag caggcagatg tatttgaaga tttatataga
     4441 gagaaactaa aagacacaaa taaattaaga gagtataatt atttacaaaa tgaaactttt
     4501 aaaagcgcat aaataggtga tgagatatgc ttaaaaaagc aaaatttatc ttaatggcaa
     4561 cgatactact atcaggatgt tcaactacca ataacgaatc caacaaagaa acaaaatctg
     4621 taccagaaga aatggatgct tcaaaatatg taggacaagg attccaacca cctgcagaaa
     4681 aagatgcgat tgaatttgca aagaagcata aagataaaat tgctaagcga ggcgaacaat
     4741 tttttatgga taacttcggt ctaaaagtta aagctacaaa tgttataggt agtggcgatg
     4801 gtgtagaagt attcgtgcat tgtgatgacc acgacatcgt atttaatgcg agtattccat
     4861 ttgataaatc aattattgac agtgatagct cattaagaag taaggataaa ggtgatgata
     4921 tgagtacttt agttggtgca gtactcagtg ggtttgaata tcgagcacaa aaagaaaaat
     4981 atgataaatt atataaattt ttcaaagata atgaagagaa atatcaatat acaggattta
     5041 caaaagaagc aattaataag acgcaaaata gtggttatga aaatgaatat ttttatattt
     5101 cggccatacc ttataattta gctgagtata gagactattt tgaaccattg ttaaacaaaa
     5161 gtgacagtga attttcaaaa gaattgtcaa atgttaagaa gcaattaaaa gataagtcta
     5221 aagtttcggt aactactact ctatttagta aaaaaaagaa ctatactaaa aaaagtaaca
     5281 gtgaaaatgt aataaaaatg gcagaagaaa taaaaaaaga taaagagata ccaaacggta
     5341 tagagcttag tataaaattt tcggacaata aaataaatac ggttaaacca aattttaacg
     5401 gtgaaagcac ttcagaatat ggtgtgtttg atcaagaata aaattaatga tgaaaattta
     5461 acggagaata gtgtatattg agtagatcaa gaataaaaag ataattctac tattgttgtg
     5521 aaggcaaata agtagaagat tttaagtgta atttctggtg atttaaataa taatataaat
     5581 ggaagtactg atataaaact ttttaaccta ctagattctt ataatttgct ttccatttta
     5641 tgacgatttt tactcaattg agtgatagaa tcaaaaaagc catctcaaaa attaatcaag
     5701 caaacaacat tccaaacaat gctcgcaaat caccaatgta tcactctcca attacgtaac
     5761 tatgatttaa tttaagcata gttattgagg ttttgtgata tatagtataa aattaatgag
     5821 aattaaattt aataatgtaa aattcatctt cggggtcggg tgtaattccc aaccggcagt
     5881 aaataaagcc tgcgacctgc tagtatgtat catattagtg gctgatctag tgagattcta
     5941 gagccgacag tatagtctgg atgggagaag atggaggttt tttgttgtgc aataatcctc
     6001 ctattcttac gagatgaatg gaaggagaaa attgaatatg caacaaaata aacgtcttat
     6061 cacaataagt atgttgagcg cgattgcgtt tgtgttaact tttatcaagt ttcctatacc
     6121 atttttgcca ccatacttaa ctttagattt tagtgatgta ccgtcactac tagctacatt
     6181 tacgtttgga ccagttgccg gtatcgtagt tgcactggtt aaaaatttat tgaactactt
     6241 atttagtatg ggcgatccag ttggaccatt tgctaacttt ttagcaggcg caagtttctt
     6301 attaactgct tacgccatct ataaaaataa acgttcaaca aaatctttga ttactggatt
     6361 aatcattgca acaatcgtta tgactatcgt gttgagtatt ttgaactatt tcgttctatt
     6421 acctttgtac ggtatgatat ttaacttagc tgatatcgca aataatctta aagtaatcat
     6481 tgtttcagga attataccat tcaatattat taaaggtatc gttatttcta ttgtatttat
     6541 tttactatat agaaggcttg cgaatttctt gaaaagaatt taatcaaatt aaagcaaaat
     6601 aatatacaca taataataaa aagcaggtga ctatcaataa acgatagctt gcctgctttt
     6661 tctatagaac atttgtctaa aaaatcaatt attcaaattt taaagcgtcc ccatcaaatg
     6721 attcgtctgc aattttaata gaatctgtag ggcatccatc aattgcatct tccatatctt
     6781 catataattc ctcaggtact tctgcagtac cttggttatc gtcaaggatt acgaaagcaa
     6841 taccttcgtc gtcgtaatca tatatatctg gcgctgctgc accgcatgca ccacatgcaa
     6901 tacaagtatc catatcaacg attgtatatt ttgccaatgt cttcgcctcc tttgataaaa
     6961 atgctaaaat agtaatgtga ctaaaatttt agacagcatc atttttattt tcaaattatc
     7021 cgttttacag agtgagggtt aaatttgcaa cacattataa aaacagcatt acaacaaaca
     7081 tttaactata aaacaaataa aagtatttac aatatcttag ttggtaagaa atctcaccaa
     7141 accttttttg acgcttgtag tcaacaacag ttgtcattat atcacagttt accactatta
     7201 aaatatccgt cttttgagct atttttagaa aaaatcaatg aatttaatgc tgaaatggaa
     7261 atcatgttgc atcctagata tacatttgaa agcatggggc aaacatttca agcaattcaa
     7321 ctattagtgc aaaccatgtc taataccaaa caacatgttt ttcattttgt accaatctct
     7381 caaaataata agatacaaga aactgtaaag attgtctata actacattaa agaaaaccaa
     7441 ttacaaattg attttgaaaa tgaactacat aacttgttta aagcaataac tttaaaaggg
     7501 ccatgttact tacattatta tttgcaaggc tatgatgaac caatgtatac gagacagcaa
     7561 gttagtttaa tagaaaagct atctcaacag caattgtttg aatacgaaat gaataattta
     7621 gtgacaatga tgtttgaatt agaaagtgga gaatatacta ttttatcaaa aataataatg
     7681 aaacctacat tattaaatca aacttatatt acttatacaa aattgcttga acaattcacg
     7741 atggaagata tagcggctca acaacaagtt aaaatcaata ctatcgaaga tcatgtactt
     7801 gaaatcttaa tcaaaggtta catgtctaat tacgatgatt atgttgaact agaagatcaa
     7861 ctccagtttt tgaattttta tcaacagcat cgtggcgaac gattaaaatt ttacaaagaa
     7921 caatttgaca cgttatcata ttttcaatta aaagtattaa tcgttggatt tgaaagaggt
     7981 gatctgaatg ttgcatgata ttttacgaaa caaatttgga ttcgagagtt ttaaaccggg
     8041 acaacaggaa attatagaaa gtataatgtc tcaacaacac actctaggta tacttccaac
     8101 tggaagtgga aagagtttgt gttatcaaat acctacgtat ttatcaggta agccgacatt
     8161 aattatctca ccgttaatat ctttaatgga tgaccaagtt atgcagttga aaataaatgg
     8221 agaaaaacgt gtaacatgta ttcactctgg tatggatgaa attgagaaaa agcataatat
     8281 taaatgttta cgacatagcc gcttcatctt tctaagtcca gaatttctcc tgcaaccgtc
     8341 aaattttaaa ttaatatcta tgatagactt tggcatgatt gttctagatg aagcacattg
     8401 cctatctgaa tggggatatg atttcagacc acattatgct ctaataggaa aagtaacaaa
     8461 gcattttaaa gaagcggttg tcttagcatt gacagcaact gcaccaccgc atttacaaga
     8521 tgatttgacg gaaatgttag cgattcaatt caatgttatt aaaactacaa tgaatcgccc
     8581 aaatataagc tttaagcatc ttaattttca tgatgatgaa gataaaattg aatggttgct
     8641 gccgtttcta caacagtcgg gaccaacgat tatttatgtc tcatcgaaaa agatgtgtct
     8701 gaatttagcg caacttattt atgattcagg ttttcttaca ggtatttatc atggtgatat
     8761 gaattatcaa gagcgacaca cagttcaaca acaattttta aataatgata ttccgattat
     8821 agtcgcaacg agtgcttttg gtatgggaat taataaaaaa gatattcgca caatcattca
     8881 ctttcatctt tcaacaagtc cttctaacta cattcaagaa attggccgtg cgggtcgcga
     8941 tggtgaacta agtcaggcaa ttagtttatt ccaaccggac gataaatata ttttagaaac
     9001 gttattattt gcagatatga taacagaaga agatgtacaa aatttcgaaa taggagaatt
     9061 tttagctccc gataaacaag ccgttttgac aacgttgcaa tcattctata gtatcggcgc
     9121 cttgaaacag atatttaagc aatcatttaa acgaaagcaa ttaggattct ttcgcatgat
     9181 tggctattgc aaattggatc aatgtagacg gaagtattta ttagaatttt tcggtgaata
     9241 tccaccggca caagatcgat gttgtgacaa tgattctaat ataactgata tcgcaatttt
     9301 aaataagaag aaggtaatta gaagtattgg atttgatgaa aagttgcaaa atttatttct
     9361 cagatagtat tactttacta aaagaaaatt gacaagctat aattagtgta tacacaattg
     9421 aaaaatgatt gaaataattt tgaaaaatat acataaacat atgtcatgtg ggtatatttt
     9481 atgtaaaatc attgtaatag aatagaaagg aagatggcta tgtctaataa ttttaaagat
     9541 gactttgaaa aaaatcgtca atcgatagac acaaattcac atcaagacca tacggaagat
     9601 gttgaaaaag accaatcaga attagaacat caggatacaa tagagaatac ggagcaacag
     9661 tttccgccaa gaaatgccca aagaagaaaa agacgccgtg atttagcaac gaatcataat
     9721 aaacaagttc acaatgaatc acaaacatct gaagacaatg ttcaaaatga ggctggcaca
     9781 atagatgatc gtcaagtcga atcatcacac agtactgaaa gtcaagaacc tagccatcaa
     9841 gacagtacac ctcaacatga agaggaatat tataataaga atgcttttgc aatggataaa
     9901 tcacatccag aaccaatcga agacaatgat aaacacgata ctattaaaaa tgcagaaaat
     9961 aacactgagc attcaacagt ttctgataag agtgaagctg aacaatctca gcaacctaaa
    10021 ccatatttta caacaggtgc taaccaatca gaaacatcaa aaaatgaaca tgataatgat
    10081 tctgtaaaac aagatcaaga tgaacctaaa gaacatcata atggtaaaaa agcagcagct
    10141 attggtgctg gaacagcagg tgttgcaggt gcagctggtg caatggctgc ttctaaagct
    10201 aagaaacatt caaatgacgc tcaaaacaaa agtaattctg gcaaggcgaa taactcgact
    10261 gaggataaag cgtctcaaga taagtctaaa gatcatcata atggcaaaaa aggtgcagcg
    10321 atcggtgctg gaacagcagg tttggctgga ggcgcagcaa gtaaaagtgc ttctgccgct
    10381 tcaaaaccac atgcctctaa taatgcaagc caaaaccatg atgaacatga caatcatgac
    10441 agagataaag aacgtaaaaa aggtggcatg gccaaagtat tgttaccatt aattgcagct
    10501 gtactaatta tcggtgcatt agcgatattt ggaggcatgg cattaaacaa tcataataat
    10561 ggtacaaaag aaaataaaat cgcgaataca aataaaaata atgctgatga aagtaaagac
    10621 aaagacacat ctaaagacgc ttctaaagat aaatcaaaat ctacagacag tgataaatca
    10681 aaagaggatc aagacaaagc gactaaagat gaatctgata atgatcaaaa caacgctaat
    10741 caagcgaaca atcaagcaca aaataatcaa aatcaacaac aagctaatca aaatcaacaa
    10801 cagcaacaac aacgtcaagg tggtggccaa agacatacag tgaatggtca agaaaactta
    10861 taccgtatcg caattcaata ctacggttca ggttcaccgg aaaatgttga aaaaattaga
    10921 cgtgccaatg gtttaagtgg taacaatatt agaaacggtc aacaaatcgt tattccataa
    10981 tataactata taaattgtaa ctgaactgct atatacaaac gtgcatatta taagtatctc
    11041 taaatatttt ggagaactta aattgcgctt ataagtatgt agcggttttt tcatttttca
    11101 aagtttgtta tttaacaagg tcttttctcg aatattggca tatcaattta actttttaaa
    11161 tagtcatcaa aaagataaaa caccacaatc aacaaattta acgaggaaga ataaaaaata
    11221 aatcaacata ttaaattgta gtgttattca actccgtagc taacaattct ctatgcacat
    11281 taaacaaatt gtcaaaaata tatcataaat cttcaagcac agacttagcg catcaatcac
    11341 tgaactgtta taatagtttg ggattaaagg aggccgaaac aatgcaaaaa gttgaaagta
    11401 tcataattgg tggagggcca tgcggattaa gtgcggctat tgaacaaaaa agaaaaggta
    11461 ttgatacctt aattattgaa aagggtaatg tcgttgaatc aatctacaat tatcctactc
    11521 accaaacatt tttctcatca agtgataaat taagtattgg ggacgtaccg tttatcgttg
    11581 aagaaagtaa accaagacgt aatcaagcgc tagtttatta ccgagaagtt gtaaaacatc
    11641 atcaattaaa agtaaatgca tttgaagaag tattaactgt taaaaaaatg aataataaat
    11701 ttactattac tacgacgaaa gatgtttatg aatgtcgatt tttaacaatc gcgacaggct
    11761 attatggtca gcataataca ttagaagttg aaggtgcgga tttacctaaa gtgttccatt
    11821 attttaaaga ggcacatccg tattttgatc aagatgttgt aattatcggt ggtaagaatt
    11881 cggctatcga tgctgctttg gagttggaaa aagctggtgc taacgtgacg gttctatatc
    11941 gtggtggaga ttattcgcct tcaattaaac cgtggatact tccaaatttc acagcattag
    12001 taaatcatga aaaaattgac atggaattta atgctaatgt tacccaaata actgaagata
    12061 ctgtgactta tgaagtaaat ggtgaaagta aaacgataca caatgattat gtatttgcga
    12121 tgattggtta tcatcccgat tatgaatttt taaaatctgt aggcattcaa attaatacaa
    12181 atgaatttgg aacagcgcct atgtataata aagaaacata cgaaacaaat atcgaaaatt
    12241 gctatattgc aggtgtaatt gctgcaggga acgatgcgaa taccattttt attgaaaatg
    12301 gtaaattcca cgggggcatt attgctcaaa gcatgctagc taagaaacaa acgcccttag
    12361 aatcataaaa ataaaggtct atgtactaaa taacttagtt ttacaacgac tgacattcat
    12421 gatatgtcaa atgaggttga tgactaatga ttgtacatag acctttttat gttacgtatt
    12481 cattataatt caaaatatga tttgatttca gctttatcta aattgttgct taacgcgact
    12541 aataatttta atcttgcttt tggaccattc aagccgttag aaaaaataaa accttgttgt
    12601 gcgagttggt aaccaccacc atcgtatgcg taagttggac tcacaatacc attaaaggaa
    12661 cgtgaaacta gcacaatagg tatatttaaa gatactaatt gttgaatgcc ttctaatgcg
    12721 cttggaggta tgttgccttg tcctaacgct tcaataacca taccatcgat accttctcga
    12781 ctataaaaac taaaaatgtc acctggcata cccatatatg cttttactaa tggtacattt
    12841 agtttgtcat tgacattttc caatgcttgt tggcgatatg gcatatgatg gaattgcaca
    12901 cgatcctttg tcaatacacc tagcggacca tgatttggac tttgaaatgt gtttgtatta
    12961 gacgtatgtg ttttggtaac attacgcgcc gtatgaattt catcattaaa tacaaccatc
    13021 acgcctttat gacgggcctt ttcatcagag gcaacgcgaa tagcggaaat ataattatat
    13081 aatccgtcag aaccaatttc attagacgag cgcattgcgc cagtaataac aacaggttgc
    13141 tcgataccta atattaaatc aagtaaaaag gcagtttctt ctaacgtatc ggtaccatgc
    13201 gtgataacga aaccatcata atatttattt gttactgctt ctaaaataat atcctttaat
    13261 tgtttaacat gttggattgt catatgaggt gatggtacat taaaaggatt taattcatct
    13321 atttgtgcat attgatttat gacatcttga tgcattgaaa tagggttgat atcatttgtt
    13381 actactttat tagattggtc ttgtgacata ctaatggtgc caccagtatg aataacaagt
    13441 agatgtttca tatatttcct cctatattta atttacctaa ttatgataaa atattattca
    13501 taaaacgaca aggaagggaa atgacgcatg aaagccatta atattgcatt agatggtcca
    13561 gctgctgccg gaaaaagtac aattgcgaaa cgtgtagcca gcgaactatc aatgatttat
    13621 gtcgatacag gagcaatgta tcgtgcatta acatacaaat atttaaaatt aaacaaaact
    13681 gaggactttg caaaactagt tgaccaaaca acattagatt taacttataa agcagataaa
    13741 ggtcaatgtg tcattttaga taacgaagat gtaacagact ttttaagaaa taatgatgtg
    13801 acgcaacatg tttcatacgt tgcatctaaa gagccagtac gttcattcgc cgttaaaaaa
    13861 caaaaagagt tagctgcaga aaaaggtatc gtaatggatg gtcgcgatat cggaactgta
    13921 gtgctaccag atgcagattt aaaagtatat atgattgcat cagttgaaga gcgagcagaa
    13981 agaagatata aagataatca attaagaggt atcgaatcaa attttgaaga tttaaaacgt
    14041 gatattgaag ctcgtgatca atatgacatg aaccgtgaaa tatcaccatt aagaaaagca
    14101 gatgatgcag tgacattaga tacgacaggc aagtcgattg aagaagttac tgacgaaatt
    14161 ttagcgatgg tgagtcaaat taaataatct aataagtcat aactatctat ttgtaatgtg
    14221 caacgcttaa cgcatataca aaatgaatgt gctgataatg atttactcaa attaaaaggt
    14281 gatttttatt caatgatgaa tgaaagttgc ctttttattt ttggtaaaag ttaatgcgtc
    14341 agtgaattgt gtaagttttt caaaaagtaa aaagaaataa taaaggtgaa ttattagaat
    14401 tccaaaaata attcattaca ttcataaagc attttacaaa tggtaagaaa atgagtgtta
    14461 caaatctaaa tattgcaaaa gaagctgatt tagtcacaaa aaatgtccta tgtaataatt
    14521 cgagaaagat gcactatata cggtcttctt actattcaaa tgtaaaagtt gcttatttgc
    14581 gtggcttttt gttttataaa agtataaaat tttactataa tatatcttgt agagaacaat
    14641 gaaatgacag acttataaaa caaaatagta agctatgcta atttttatac aaaaaatatg
    14701 tataaacata gcaaactgta ttactttgat acaaaaatgg ttgtaataaa tatttatcga
    14761 tatgac
//
LOCUS       DANJFZ010000063        14729 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.063, whole genome shotgun sequence.
ACCESSION   DANJFZ010000063 DANJFZ010000000
VERSION     DANJFZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.063"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..320
                     /locus_tag="PWN45_001683"
     CDS             <1..320
                     /locus_tag="PWN45_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623452.1"
                     /translation="AIGALYLPVIFKVIKLQLNLSKGLIDDVNAQTFIKSNQSGIVIS
                     VICCLLITAILYYTLDGFYASLTGVLGTLVALNPYTTIVSAVLYILTAIATVEATKTK
                     YRN"
     gene            516..1901
                     /gene="fumC"
                     /locus_tag="PWN45_001684"
     CDS             516..1901
                     /gene="fumC"
                     /locus_tag="PWN45_001684"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL0623453.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2364..3185)
                     /locus_tag="PWN45_001685"
     CDS             complement(2364..3185)
                     /locus_tag="PWN45_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0623454.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3348..4460
                     /locus_tag="PWN45_001686"
     CDS             3348..4460
                     /locus_tag="PWN45_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDL0623455.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4482..5105
                     /locus_tag="PWN45_001687"
     CDS             4482..5105
                     /locus_tag="PWN45_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0623456.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5461..5925
                     /locus_tag="PWN45_001688"
     CDS             5461..5925
                     /locus_tag="PWN45_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0623457.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            6104..7228
                     /locus_tag="PWN45_001689"
     CDS             6104..7228
                     /locus_tag="PWN45_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDL0623458.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            7297..7641
                     /locus_tag="PWN45_001690"
     CDS             7297..7641
                     /locus_tag="PWN45_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDL0623459.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            8024..8122
                     /locus_tag="PWN45_001691"
     CDS             8024..8122
                     /locus_tag="PWN45_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623460.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            8413..9609
                     /locus_tag="PWN45_001692"
     CDS             8413..9609
                     /locus_tag="PWN45_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL0623461.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            9599..12535
                     /locus_tag="PWN45_001693"
     CDS             9599..12535
                     /locus_tag="PWN45_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0623462.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12532..13473
                     /gene="yhaM"
                     /locus_tag="PWN45_001694"
     CDS             12532..13473
                     /gene="yhaM"
                     /locus_tag="PWN45_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDL0623463.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(13594..14556)
                     /locus_tag="PWN45_001695"
     CDS             complement(13594..14556)
                     /locus_tag="PWN45_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDL0623464.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         5433 a         2094 c         2518 g         4684 t
ORIGIN      
        1 tggcaattgg ggcattatat ttacctgtta ttttcaaagt aataaaatta caactcaatt
       61 tatcaaaagg attaattgat gatgttaatg cgcaaacatt cattaaaagt aatcaatcag
      121 gtattgtaat cagtgtaatt tgttgtttat taataacagc aatattgtat tatacacttg
      181 atgggtttta tgcgagttta acaggagttt taggtacgct tgttgctcta aatccatata
      241 cgacaattgt tagtgctgta ttatatatac ttacagctat tgctacggtt gaagctacta
      301 aaacaaagta tcgtaattaa acttagttca ataataacta aataatgata gataacacac
      361 taatcgtatg accgtatcac tgtaataaag ggtgaacacg tgcgattagt gtatttttat
      421 ttcaaattat gaaaacgttt taatcataaa ttgctcaatt aaattattta atgatatact
      481 acagttaaat aaacagtgat aaggggagaa attgaatgtc agtaagaatt gaacatgata
      541 cttttggaga aatagaagta cctgcagata aatattgggg tgctcaaaca gaaagaagta
      601 aacgtaattt cccagttggt aaagagcgta tgccaatcga agtagtttat ggttttgcac
      661 aactaaagcg tgcagcagca atagctaatt ttgatttagg aaaattaagc gaggcaaaga
      721 aagatgccat tgtatacgca tgtgatcaaa ttttatcagg tgaattagat gaacacttcc
      781 cactagttgt atggcaaaca ggaagcggta cacaaagtaa tatgaatgtg aacgaagtag
      841 taagttatgt tgctaatatg tatttaaaag atcatcaaag tgatgaaagt atccacccaa
      901 atgatgatgt aaataaatct caaagttcga atgatacatt cccaactgct atgcacgttg
      961 cattatatca agaggttgaa acaaaattag aacctgcatt aaaactttta agaaatactt
     1021 tgaaagaaaa agaagataaa tttgattcaa ttattaaaat tggtcgtaca catttacaag
     1081 atgcaacgcc gatcaaacta ggacaagaga ttagtggctg gcgttatatg cttgaccgtt
     1141 gtgaaacaat gttatctgaa tctaagaagc acattttaaa tcttgccatc ggtggtacgg
     1201 ctgttggtac tggtattaat gcgcatcctg aatttggtga taaagtggca cattatattt
     1261 cagaaaatac gggttatcca tttgtatctt ctgaaaataa attccacgca cttacagcgc
     1321 atgatgaagt tgttcaattg catggaacat tgaaggcatt agcaggagac ttaatgaaaa
     1381 ttgctaatga tgtgagatgg ttggcttcag ggccacgagc tggtttggca gaaatttcta
     1441 tccctgaaaa tgaaccaggt tcatcaatta tgcctggtaa agttaatcct acacaatgtg
     1501 aaatgttaac aatggttgca gtccaagtaa tgggtaatga tacagttgtt ggcttcgcaa
     1561 gttcacaagg taactttgaa ttgaatgttt ataaaccagt tattatgcat aatacactac
     1621 aatcaattta tcttttagct gatggtatgg aaacatttaa taacaattgt gcagtgggca
     1681 ttgaaccaat cgaagagaat attgataatt atttaaatca atcattaatg ttagttactg
     1741 cattaaatcc acatattggt tatgaaaaag cagctcaaat tgctaagaaa gcccataaag
     1801 aaggtttaac tttaaaagaa tctgcaattc aaactggata tgttacagaa gaacaatttg
     1861 aagcatggat taaaccagaa gatatggtag atcctcatta attaaatatt aatataaaat
     1921 aaagtttggg cattacaggt agatgtagtg ttcaagcttt attttttgtc tatgtcaaat
     1981 tgaacttata agtctgattt gatatagagc catgaaaagg aaaaactttt catggagtcc
     2041 gttttattat agaacctatt ttttatatga tgaattgttg tacatgtatt ggccaaacaa
     2101 cctgtgataa aaatcttatg atgtcatgta ttgtccatag atacacgtaa cgcagttgaa
     2161 aaagactaca agtcattatc caaggatgtt ataaaatgaa taattactaa aatatcaaaa
     2221 tataaaatcc taagcatcgt aaaaaacaaa aaaaccgaac atctgaaaaa attaataatt
     2281 ttccatctat ccgatttaag ttgatacttg atttagttac aatgcggtat tgcatgttta
     2341 aatatcatgc aaaactaatc aaattagtca tccaaagaaa cggaaatgac ttccttagtc
     2401 aagggatgca caaactctat tttgtaactt tcaagttcta attgtcttaa cgttgaatca
     2461 ccatataaag gatcaccaat aacagggtga ccaatttcag ctaaatgcac acgaatttga
     2521 tgagtacgtc cagtatccaa cttgatttca agttggcaca cagcttcttt tatcattttt
     2581 gaagttaaaa tgtgtgtaat tgcacgctgt ccagtaggag acacacgtcg tttattcgaa
     2641 tggaatttat ctttaccaat tggcatatca atcgtttgtg gttttaaagg taaaagtgcc
     2701 ttaacatttg ctttgtatat ccgcgtaata tcattgtctt ctaacatgcg atcaagaatt
     2761 tttttcatta aaggattttt agcaacaatt aataatccta ctgtttcctg gtccagtcga
     2821 tgaattggtt cgacatagtc actatcaata gtgtaaatca catgattcat taaagtattg
     2881 ctttctttta aatcattagg gtgagtctta acacctttag gtttcattac gattgccata
     2941 tcatcgtctt cgtatttaat ttgtgcataa cgataacttg gaacataatt acttttttca
     3001 tcgatggttg gaacaaatac atcgtcgcca gtatgcactt tatccattaa tcgcgcaggt
     3061 ttatcattaa tagtaatatc tttagacata tttaaatgat gtaaatcttt tttaggtacc
     3121 ttaagttgtt ggaaaatatc tcgtaaactt aagtcattaa agttttctgg tattttaaat
     3181 ttcataaaat cctcctatag tctttaatat catatcacaa atcagttctc cgaaatataa
     3241 ctattgagta atcaaggcca ttttatcatg tttacgtcta agatttaaaa atataataac
     3301 tcaatgtata atagttaata cacgtaaaaa tgaattggag cgatttgatg gaacaaagga
     3361 cgcgactagc tttattaaaa gagattgccg aatttttaaa cgaagaaact gaaatgtata
     3421 gcatgaccca aggtgcgtta aaatatttaa ttgagggcag taatttcacg acaggctgga
     3481 tatttttcat caatagcgta ggtgaacatg aacttgtatc acatgtggct ctaccacaat
     3541 ctttgacagc agaccactgt cactatatta aagatgggtc ttgttggtgt gtgaaagcat
     3601 ttaatcaacg taggctaatg aaagcgtcga atatcgtcaa ttgttctcgt attaacttag
     3661 cctctaaagc atttcctagc caaaatgaca atattacaca tcatgcaacg gtaccactta
     3721 aatcgggtca agaacaattc ggcattttaa atgtagcgtc tccaaatact gaaatatata
     3781 gcgatgaaga tttagagttg ctagagtctg tcgcatttca attagggtca gccatcaaac
     3841 gtatttattt aacagaccgt gaaaaagaag cggcaaaaat taatgaacgt aatcgtttag
     3901 ctagagattt acatgattca gtaaatcaaa tgttattttc tgtaaaacta acagcacatg
     3961 ctgcgtatgg catgtcaaac gaatccatcg ctaaacaagc attcaagacg attgaggaaa
     4021 cgagtcaaaa tgccgttaat gaaatgcgtg cattgatttg gcaacttaag ccagttggac
     4081 ttgagcaagg tttaattcat gctttgacag catatagtaa attaatgcat atacaattaa
     4141 atgtaaatgt agaaggttta atcgatttat ctaatgaaat cgaagaaaac atatacagag
     4201 cattacaaga gtgtattaat aatgttaaga aacatgctga tacgaataaa atggatctta
     4261 cattaaaaca aatgaacgat atactatata ttgacgtgat tgattatggt caaggatttg
     4321 agatcgataa tgttcagata gcttcatcac acggtattaa taacataaaa caaagagtta
     4381 aattattaag aggtaaagtg acctttcatt ctcagccaac aaagggtaca caaatacaat
     4441 tcacaattcc tataaaatag cttgatttaa ggagataacc catgaacaaa gtaatattag
     4501 tagatgacca ttatattgtg cgacaaggat tgcgattttt attatccacg attgaaaaca
     4561 tagaagtttt acaagacttt gcagatggag aaacattttt agaatattta aaagagcatg
     4621 agcaccctga tattgtgcta ttagatttag tgatgcctgg catgaatggt attgaaatta
     4681 cggaatatat taaggcacat tatccggata ttaaagtttt ggtattaaca agttatgttg
     4741 atgatgaaca tgtaatttca gcaatcaata aaggtgctga tggttatgaa atgaaagacg
     4801 ttgagcctca gcaattaatt gaaactatta gacgagttat gaacggtgaa aaaatgatac
     4861 atcctaaggc acaagatgta ttcgaaacag ttagccaaaa accacactac acgaataagt
     4921 tgtcaaagag agaaattgaa gtgttacgtg aaatggttaa aggtaaaaca aataaagaga
     4981 ttgcagaaac tttatttgta tctgaaaaaa caattaaaac acatgtcagt catatattta
     5041 gtaaattaca agttagcgat cgtacacaag cagcaattta tgcaatggaa aataagttga
     5101 tttagaatga gcttttaaat atttgtcgaa aaatagacaa tttttaatct gattttgatg
     5161 aattttttat agcgataggg tttattatac agttttcttt gtaaatttga tatcattata
     5221 aaagtgtaag ttttatcaaa tctagtgatt atgcataaaa atttacttca tttactttcg
     5281 cttcattata aagtaaactt aactcttgtt gacttgatca aaatttaata caaatgtgaa
     5341 attgactctt ttcattttat taaaaatata ttaagatgta atctgtgttt caagaaatgt
     5401 gtattattgc aatttcttgt gtccggataa aattccccaa acagaaaggt agataacaga
     5461 atggatagac agagttttac agatttaatt caaacaaaat ttaaaatggt tcgtatagag
     5521 gctggttata cgcaagatac tatggcgcaa acaattggac tttctaaaaa gactttagta
     5581 caaatcgaaa aagagagagt attaccaaac tggacaactt gtatttcaat ttgtgcatta
     5641 ttcagagact ctgaagtatt aaacagtaca tttggctgtg atccattaga aatcgttcaa
     5701 acaatttcaa gaaatcactg tgcatatcca aaccatgcac caacaagtga tatttattgg
     5761 aataacattg agactcgtaa tgggtacatt ttacaaagta acaaagtaag caatatttat
     5821 cgtgtattaa atccagataa ccaaccaatt ttcggtactt ctaaaatgag agaagctgaa
     5881 acatacttta acagaaatgc taaagaagaa ttagtacata tctaggccta tatactttta
     5941 ataaataata aaagcgatgt aacacacctg tttcgagagg tgaggcatcg ctttttgtat
     6001 gtaaactaat aataaattga gcagaaagtg aaatgattaa tgaattaaaa aataaaaata
     6061 gtggtataat gaataactat gttgtcacaa aggagtcaaa attatgaatg cactatttat
     6121 tatcatattt atgattgtag taggggcgat aattggtggc attactaatg taattgcaat
     6181 tagaatgctg tttcacccct ttaaaccata ctatatattt aaatttagag tcccatttac
     6241 accaggttta ataccgaaaa gacgcgaaga aattgcaact aaaattggcc aagtgattga
     6301 agagcatttg cttacagaaa ctttaattaa tgaaaaattg aaaagcgagc aatcacagca
     6361 agcaatagaa tctatgattc aacagcagtt acaaaagttg acgaaagatc aattgtcaat
     6421 aaaacaaatt acttctcaaa tcgatattga tttagaacaa gtattacaaa ctaatggaaa
     6481 tcaatatatt gaatcacaat tgaataatta ttatacaaag catcaaaacc aaacaatagc
     6541 atctttattg ccaaatcaac ttgtaacatt tttaaatcag catgtagata acgcaacaga
     6601 cttattatgt gatcgtgcaa ggaattattt atcatctgca aaaggcacac aagatattaa
     6661 tgatatgttg gatacttttt tcaatgagaa aggtaagtta attggtatgt tgcaaatgtt
     6721 tatgacaaaa gagagtattg cagatcgcat tcaacaagaa cttatacgtt taacatctca
     6781 tcctaaagca agaacaattg tgacatcgtt aattaccaat gaatatcaaa cttttaaaga
     6841 taaaccattg aatgaactgt tagatgcatc gcagtttaat gaaatcgctg aaaatctatc
     6901 agtgtatgta acaacatatg caagcaaaca agcgaataag ccagtggtta cgttaatgcc
     6961 tcaattcgtt gattatttag aaggtcaact ctctagcaaa ttagcaaatt taatcattga
     7021 aaaattatcg attcacttat ctacaattat gaaaaaagtg gatttacgag gattaattga
     7081 agaacaaatt aatacgtttg acttagatta tattgaaaaa ttaattatag aaattgctaa
     7141 caaagaacta aagttgatta tgtcattagg tttcatttta ggtggtatta ttggattttt
     7201 ccaaggtttg gttgcaatct ttgtctaaaa aagcgagatg tggtaaagta atatcgaacg
     7261 ttttaataaa catcacaaca aaggagtgct ataaccatgg cagtaaattt atatgattat
     7321 gcaaatcaat tagaacaagc tttaagagaa agcgaagaat acaaagcaat caaagaagca
     7381 ttcgctaatg taaaagctaa cgaagaatct aaaaagttat tcgacgagtt ccgtgaaact
     7441 caaattaact tccaacaaaa acaaatgcaa ggtgaagaaa ttgctgaaga agatttacaa
     7501 aaagcgcaag aacaagcgca agcaattgaa aaagatgaaa acatctctgc attaatgaat
     7561 gctgaacaaa aaatgagtca agtattccaa gaaatcaacc aaattatcgt taaaccatta
     7621 gacgaaattt acgctgacta atattaaaat aatatttttc aatcctttaa atagaggaat
     7681 agactgccga cagagtccga gactttgtgg gtagtttttt tagttttgat aacggaagtt
     7741 agaggctctc tgtcaaattg ggcagatgca ttcagtaacg gaagttaggc tagtaaacgt
     7801 gtcctaactt cctttttgca ttttgaagtg taaagttttc aacataatac tattagttcg
     7861 gtcatgtatc ggactgatgg aaaagcgttt cacttttaat gactcattaa gaacggcctg
     7921 aaaatgtttg gcgtattaag tgcaatgata gttttgacat ttagtttcta attggtcatt
     7981 actgccgagc aaatctagta gagtaatcat gtaaatcttt aatgtgccat ttgattcact
     8041 agcggtgtta ataactacgg aaattgcatt tccgactgaa atttttgaaa aatatcaacg
     8101 tacgctacaa ataaaatttt aaactgttat aaatgtgtct caatttcata tgttcatcga
     8161 cgatatgaag cgtattatgg taaaatgaag aaataataaa cttgttaata aataaaacat
     8221 cacgatttga ctaaagcact ttattattgt gtagataata gttttttaac gaaataaaaa
     8281 tggcgactgg ttttaataaa tcagctaatg aatcactaca cctataagta tgaatatagt
     8341 gattagaatg ctttgtatag ttggattttg caaaattgat gttagaacct ttaattaagg
     8401 agatatggat gtatggttaa atttattcat tgttcggatt tacatttaga tagtcctttc
     8461 aaatctaaga gtcatataag ccctaaaatt tttgaggatg ttcaaaaaag tgcttatgaa
     8521 agttttaaaa atattgtaga tattgcatta caacaagatg ttgattttgt aattatcgcg
     8581 ggtgatttat ttgatagtga aaacagaaca ttacgtgctg aaattttctt aaaacagcaa
     8641 tttgaacgtt tacaaaatga acaaatcttt gtttatgttt gtcatggtaa tcacgatcct
     8701 ttatcatcaa agatttcatc aaactggcca gataatgttt ctgtattttc aaataaagtt
     8761 gagacgtatg aagcaattac taaatctggt gaaacaattt atattcacgg atttagttat
     8821 gaaaatagag caagttatga gaacaagatt gatgaatatc catcaagtca aggccaaaaa
     8881 ggcatacata ttggtgtctt gcatggtacg tatagtaaat cttcagttaa cgaaagatat
     8941 accgagttca ttttagaaga tttaaacagt aaattgtatc attattgggc tttaggtcat
     9001 atacatgaac gtcaacaatt aagtgatatg cctgtaatta actattcagg taatattcaa
     9061 ggtagacatt ttaatgagca aggtgaaaaa ggttgcttat taatcgaggg tgaccactta
     9121 aaattaaaga ctaaatttta tcctacacag tatattagat ttgaagaagc aactattgaa
     9181 acggataaga catctaagca aggtttatac gaggtcattc aaaactttaa agaacaagtg
     9241 agagaagaag gaaaagcctt ttatcgttta acgcttgtta ttaatagtga gacattaatt
     9301 tcacctcaag atttattaca agttgaagaa atgattacag attatgaaga aaacgaaaat
     9361 caatttgtat atattgatga gttaaaaata caatatgcac aaaatgatga gtcaccttta
     9421 gttaatgaat tttcagcgga attattagtc gatcaaactg tttttgataa agcgatgtca
     9481 gatttatatt taaatccaag ggcatctaag ttcctagacg attatggaac attcgaccat
     9541 acagcattag ttaatcgtgc tgaagaaata ttaaaagctg aaatgagagg tgaacaaaat
     9601 gataattaaa tcacttgaaa tttatggtta cggtcaattt gttcaacgta aaattgaatt
     9661 taataaaaac ttcactgaaa tttttggtga aaatgaagcg ggtaaatcga cgattcaagc
     9721 attcatccat tcgatattat ttggatttcc aactaaaaag tctaaagagc caagactaga
     9781 accacgtcta ggtaaccaat acggtggtaa attagtactt attcttgatg atggcttaga
     9841 gattgaagtt gaacgaatta aaggcagtgc tcaaggtgat gtgaaagtat atttacctaa
     9901 tggtgctgtg cgtgatgatg cttggttaca aaagaaactt aattatattt ctaaaaagac
     9961 atatcaaggt atcttttcat ttgatgtact agggcttcaa gacattcata gaaatctaaa
    10021 tgaaaaacaa ttgcaagatt atttattaca agcaggggct ttaggatcaa ctgaattcac
    10081 gtcaatgcgc gaagtgatta atcgtaaaaa agatgaatta tataaaaaat caggtaaaaa
    10141 tccgatcatt aatcaacaaa ttgagcaatt aaaacaacta gaaagtcaaa ttcgtgaaga
    10201 agaagcaaag ctagaaacat atcatcgctt agtagatgat cgagataaat catcacgtcg
    10261 attagagaat ttaaagcata atttaaatca attatcaaaa atgcatgaag aaaaacaaaa
    10321 agaggttgct ttacatgatc attcacaaga atggaagtct ctagaacaac agttaaatat
    10381 tgagccaatc acattcccag aaaaaggtgt ggatcgttac gaaaaagcac gagcgcataa
    10441 gcaatcgtta gaaagagata ttggtttaag aaatgagcgt ttagctcaac ttaaagaaga
    10501 agcgactcaa ttagagccag ttaaacaatc tgatattgac gccttcatta gtttgaatca
    10561 acaagaaaat gaaattaaaa ataaagaatt tgaacttact gcaatcgaaa aggatattgc
    10621 gaataaacaa cgtgataaag atgaattgca atcaaatatt ggttggtctg aaacgcatca
    10681 tgacgtagat agttcagagg caatgaaaag ttatgtcagt gagcaaatca agaataaaca
    10741 agaacaagct gcatacatta aacaattaga acgtagttta gaagaaaata aaatcgaaga
    10801 taatgcggtt catagcgaac tagattctgt tgaagaaaaa atagttcctg aagagacttt
    10861 tgaaaagaaa aaagaatact cacaacaagt cattgaatta aacgaaaaag agaacttgta
    10921 tagcaaattg aaagaacgtt tcgaaattga gcaacaagaa aaacaaaaac gtcaaaaatt
    10981 gttacgaaca acgtttattc ttttaacatt agtaggtatt ggtttaactg ccttctcatt
    11041 tatttcaaac aacatgttgt ttggtattat tttcgcagta ttaactttag tatttgttat
    11101 cggtatcatc atgtctaaat caaaagaagt agattatagc gaggccatca cagatgaaat
    11161 cgaagaaatt aaagcacaac tagccatttt agatgaaaat tatgacttag attttgatct
    11221 tgatgagcaa tatcgcattc gtgatcactg gcaacaagct ttgaaaaata aagatatttt
    11281 agaagagaaa cgtcagtata ttgaaggtcg tttaaatgat gcgaaagggc gtcatgatga
    11341 attacaatcg acagttgaga acgttaagga tgaattatac ttatcatcta aaatttcaaa
    11401 tgatttaatt gttgatagta tttctacaat ggcgaatatt aaagcattag atcaacatat
    11461 cagtgattta aatcaacaac gccagcaatt ggttcaagaa ttagatacat tctacaatca
    11521 tgctgaagct gttacaaaat cacaatttgt ttatttcaac aaattatctt tattccacga
    11581 tgtgcaacaa tggttgaaat cagctgaaga cacgaatgaa aaatggcgta ttaatgctga
    11641 aaataccaaa cttgttacaa atgaattaaa tcatttaaat gctcaattag aagaaaataa
    11701 taaagaaatt acagcactat tcgattttat caatgttggt actgaagaag atttttacca
    11761 acatcatgaa gattaccaaa cttatactag taatttgagt cgttttaatg atttaactaa
    11821 atatcttgag aaccaaaact attcttatga attaagttct agtttaagtg aaaaaacaac
    11881 tgcacaactt gaagaagaag atcatttatt ggctactcaa gttgacgaat ataatgagca
    11941 atatcttgaa atgcaagcac aagtcagtga tttaagtgca caaatcaatc acatggaaac
    12001 tgatacaacg cttgctaatt taagacatga atatcacagt cttaaaaatc aacttaatga
    12061 tatcgcaaaa gattgggcaa gtttaagtta tttacaaagt ttagttgatg aacacattaa
    12121 acaaattaaa gataaacgtt tgcctcaagt tattaatgaa gcggtagaaa tattgaagca
    12181 tttaacagat ggcagatata cgatgattaa ctataatgaa gattcaatta cggttaaaca
    12241 tgttaatggt caattatatg atcctgttga actaagtcaa tctacaaaag aattacttta
    12301 tgtagcttta cgtatcagtt taattaaggt actaagacca tattatccgt tcccattaat
    12361 tgttgatgat gcatttgttc attttgataa aaaacgtact gaaaaaatgt tgaattattt
    12421 acgatcatta tcagaacact atcaagtact atactttaca tgtgtaaaag ataatattgt
    12481 tccatcaaaa gaagtgatta cattaaacaa aatagaggaa ggcgggaaac gatgagaaat
    12541 atagagaatc taaatcccgg agattcagtt gatcactttt tcttagtgca taaagctaca
    12601 cagggtgtaa cagcacaagg taaagattat atgacattac atttgcaaga taaaagtggt
    12661 gaaattgaag cgaaattttg gacggctaca aaaaatgata tggcaacaat caagcctgaa
    12721 gaaattgtac atgttaaagg tgacatcata aactatcgcg gaaataaaca gatgaaagtc
    12781 aaccaaatta gactagcgac aactgaagat caattaaaaa cagaacaatt tgtagatggt
    12841 gcacctttat caccggcaga aatacaagaa gagatttctc attatttgct agatattgaa
    12901 aatgctaatt tacaacgtat cacacgtcat ttattgaaaa aatatcaaga acgattttac
    12961 acatatccag ctgctagttc tcatcatcat aactttgcga gtggcttaag ctatcatgta
    13021 ttaacgatgt tacgtattgc aaaatcaatt tgtgacattt atccattgtt aaacaaaagt
    13081 ttgttatata gtggtattat tttgcatgat attggtaaag ttagagaatt gagtggtcct
    13141 gttgcgacgt cgtatacagt cgaaggtaac ttattaggac acatctcgat tgcgagtgat
    13201 gaagtagttg aagcagctcg tgaattgaac attgaaggag aagaaatcat gttgttaaga
    13261 catatgattt tatctcatca tggtaagtta gagtatggtt ctccaaaact gccatactta
    13321 aaagaagcag aaattttatg ctatatcgat aatatcgatg ctagaatgaa tatgtttgaa
    13381 aaggcatata aaaaaactga caagggtcag tttacagata aaatatttgg tcttgaaaat
    13441 cgtagattct acaatcctga atcactcgat taagaattaa aagatatcgg acagatgagt
    13501 atactgaaac atgagctcat ctgtccgatt tttaatgcga aaaaaccgag cggccgtggt
    13561 atgatttttg aaccacggtc gctcggtttt gtgttattgg ctcatgccgg attgtccgcc
    13621 ttgtgcgcca ccttgtttaa gtttttcagg gtttaagatt ttatcttcga caactgattt
    13681 aatatcacga tctttaaagt caacatcgta ttcttttaat agatctttgt atgcatcagt
    13741 caataatttt ggattttttt gtactttctg atcgactaat ttttctttca ggctttgttt
    13801 ttcactgtta aagtctgttg gtttatcagc tttaataata tgatatccaa agcttgattt
    13861 aacaacctct gatacttcac catctttaag cttaaatagt gctttttcaa aatctttatc
    13921 agtttgtcct ttaagaacat aacctaattc gccatctttt ttagctgaac cagtatccat
    13981 tgattctttt ttagcgattt caccaaattt acttggatct tttgaaactt ctttttgaat
    14041 ttcttcagct ttttgtttcg cttctttatc atctaagcct tctttgtcgc ttttcttaga
    14101 tttaacttta attaaaatgt gtgaagcttt cttgctgtct tctttaattt cagaatcaga
    14161 gattttaatt ttatctgata gtaattcttt atgataagca gcagtacgta aattttcttt
    14221 atatttatcg gctgttaaac cttgctgttg aagggccttt tcaaatttat ctttaccgcc
    14281 gtattgcttt tgcatttttt caatttgttc gtcaatcttc ttatcattaa ctttattttt
    14341 atatttatca gctaaaattt tatttaacat ttcagtaaat gatgcatttg caatttgatc
    14401 tttaccgatt tttttcattg tatctgcaac tgttacgtct ccagctttag aagaaattaa
    14461 tgtattttct ttagagtctg tggcactagc gccacaagcg cctaataata aagcactagc
    14521 tgttaccgga acgattaatt tgtttatcat cttcatagtt gaaactcctt tgtaagttaa
    14581 tcatcgtgaa tataacatag ttttatatta tcaccaatca ataacccatg acatcagact
    14641 agagcattag gtttaggggt agaaattgat gaaaataaaa aaacaaggtc tatatacatt
    14701 tgattgtaag ctattacata gcctaaaaa
//
LOCUS       DANJFZ010000064        13910 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.064, whole genome shotgun sequence.
ACCESSION   DANJFZ010000064 DANJFZ010000000
VERSION     DANJFZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.064"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(699..896)
                     /locus_tag="PWN45_001696"
     CDS             complement(699..896)
                     /locus_tag="PWN45_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623465.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(1389..2729)
                     /gene="glnA"
                     /locus_tag="PWN45_001697"
     CDS             complement(1389..2729)
                     /gene="glnA"
                     /locus_tag="PWN45_001697"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL0623466.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2748..3116)
                     /locus_tag="PWN45_001698"
     CDS             complement(2748..3116)
                     /locus_tag="PWN45_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0623467.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(3359..4597)
                     /locus_tag="PWN45_001699"
     CDS             complement(3359..4597)
                     /locus_tag="PWN45_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0623468.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4616..5854)
                     /gene="hflX"
                     /locus_tag="PWN45_001700"
     CDS             complement(4616..5854)
                     /gene="hflX"
                     /locus_tag="PWN45_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL0623469.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5966..6442
                     /locus_tag="PWN45_001701"
     CDS             5966..6442
                     /locus_tag="PWN45_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL0623470.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6664..6897)
                     /gene="hfq"
                     /locus_tag="PWN45_001702"
     CDS             complement(6664..6897)
                     /gene="hfq"
                     /locus_tag="PWN45_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL0623471.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6912..7847)
                     /gene="miaA"
                     /locus_tag="PWN45_001703"
     CDS             complement(6912..7847)
                     /gene="miaA"
                     /locus_tag="PWN45_001703"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL0623472.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7865..8779)
                     /locus_tag="PWN45_001704"
     CDS             complement(7865..8779)
                     /locus_tag="PWN45_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623473.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8929..10602)
                     /locus_tag="PWN45_001705"
     CDS             complement(8929..10602)
                     /locus_tag="PWN45_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL0623474.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10760..12256)
                     /gene="glpK"
                     /locus_tag="PWN45_001706"
     CDS             complement(10760..12256)
                     /gene="glpK"
                     /locus_tag="PWN45_001706"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL0623475.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(12385..13203)
                     /locus_tag="PWN45_001707"
     CDS             complement(12385..13203)
                     /locus_tag="PWN45_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0623476.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(13668..>13910)
                     /locus_tag="PWN45_001708"
     CDS             complement(13668..>13910)
                     /locus_tag="PWN45_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDL0623477.1"
                     /translation="IFRVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKE
                     TNTQVIAGGLINTIDEVNEAVKNGAKYVTTSYDKLW"
BASE COUNT         4303 a         2649 c         1901 g         5057 t
ORIGIN      
        1 tttttgttaa aatgaaagaa taatttattt ttttctagtt aaattaataa taaataatat
       61 taaagttgca atgttaagat agagagttaa ttttttcctt aattctcttg tcataaatac
      121 cacctcccta tttaatgtgg taaaggtctt ttgcccttta cctttttaaa agaacgattt
      181 gataaaatta agtacttgaa ttattaagct cgtcacagct attcgaaatt taattttgct
      241 attcgttctt tttctttaac cattcttatc attttttctc ctttcctctt tatagttatt
      301 taacattcca caaattgggg taagtgacat gttgaactcc catatccact ggcaagattt
      361 ataatttgta attctaaagt ataatagcct atatttttca aaatctcttt ttttatgatt
      421 aacatccctc taagtgcacc taaaggtttt tatgtaatgt attaaatatt tccatttata
      481 catatacatc ttgtataaaa agaaagaact ccatatatac tcaaaaggta tactgaagct
      541 ctatatatta tataaaatta ttatactatt ttgaaaaaca taagtaaatc actaaacatg
      601 atttctttca cttttagaaa acttttaata ctataaaagc acccactcag tcactagttt
      661 gggcagttat tgtatgccta ttgaactcaa tgcttatatt acaatacctt tttcgcatat
      721 tcatataaga tctttgcatt ctttaagctt aaattttcta ttcttctttc tctacggcgc
      781 atagcattaa tattaccgta acttatccca gtatctttat taatttgata actcgatatc
      841 tctttgtttt ctatcaattc tttgattgta ttgaatattt catcatagca attcataaat
      901 tagatgaggc gaaattttta attttttaga atatcaatag tagtataact aaaatgaaaa
      961 taccgatcga taaacaaaaa gatatttttt gttttgtttc tcttttcata tagtattacc
     1021 cccttaataa tgcgtagtaa ggtccctctt ttcggggtct taccttagaa cgttctgcaa
     1081 atgaattcga tgagaagtaa tatgaatatg gctattttca agtaatactc aacgttttcg
     1141 cgacgttctt ttatcgcctc atctcatcac ctccaaatat attaaaattc atgtgaacta
     1201 aaatataaaa tggtcttccc cagctttaaa aaaataaata cataaaacat ttacttgacc
     1261 aaaacttgac caaatccatt ttttaacaaa agtggtcaag tgaaatccca tctttccaaa
     1321 tcaaaaaaga gctaaagcaa aatgctctag cccttgatat tactgatttc ccatcattgt
     1381 ttagcgtatt aatattgctt catgtactga tctctttccc attcagagac ttgagttctg
     1441 tagtaatccc attcaattga ttttgaatta ataaattgat tatagatatg atttcctaaa
     1501 gcttttttaa taacttcatt ttcacgcatt gcttttaatg cagtataaag tgttgaaggt
     1561 aagtcttgaa tgcctactgc ttcacgttct tcacggttca tttcgtaaat attttggtta
     1621 actggttctg gaacttttaa tttattttta ataccatcta gtccagcttc taagattgca
     1681 gctaacgcca tgtatgggtt tgcagctgga tctactgaac gtacttcgat acgagtagat
     1741 aatcctcttg aagatggtac acggattaat ggtgaacggt ttttaccact ccatgcaata
     1801 taacatggtg cttcataacc aggtactaaa cgtttatatg agtttactaa cgggttacat
     1861 acagcagtaa atccgcgtgc atttttaagc acacctgctg taaattgata tgcagtttcc
     1921 gttaagccca tttcagtatt tggatcaaag aatgcatttt ctttaccttt gaataatgaa
     1981 acgttaaagt gcataccgct accattcaca ccgaataatg gtttaggcat aaatgttgcg
     2041 tgtaaattat gtttacgtgc gattgtttta acaaccaatt taaatgtttg gatattatca
     2101 catgctgtaa cagcatctgc atatttaaag tcaatttcat gttgaccagg ggcaacttca
     2161 tggtgactag cttcaatatc gaagcccata tcctctaatt ctaaaacaat atcacgacga
     2221 cagttttcac ctaaatctgt aggtgctaaa tcgaaatatc caccatcatc attaagttct
     2281 aaagttggtt cccctttttc atccaactta aacaagaaga attctggttc aggccctagg
     2341 ttaaagtctg tgaagcctaa atcttccatt tcttttaata cacgttttaa gtttgcacga
     2401 ggatcccctt caaatggtgt tccatctgtt ttatatacat cacaaattaa acgtgcaact
     2461 ttaccttgtc cagcagtcca tgggaagatt acccaagtat ctaaatctgg atgtaagtac
     2521 atatctgatt cttcgatacg tacgaaacct tcgatagaag aaccgtcaaa catcatttcg
     2581 ttatcaagta ctttttctaa ttggcttaca ggcacttcaa catttttaat tgttcctaaa
     2641 atatcagtga attgtaatct taaatatctt acattttcct cttctgcaaa tttacgaatg
     2701 tcgtctttag tgaaagtacg ttttggcatt ttaaaatcct ccaaatttta tttaataaat
     2761 cgggataaat caccacgatt tattggcaaa gtttctccaa taggcttttg cgtggcatct
     2821 acaatcatct tttttcttat ctcttgttca tctgttgtta aatgctcttg tgagtcataa
     2881 atgatttgtt taatcccttt gatattaaat cctttttcta ataatgattt aatttctagt
     2941 aatctttcca aatcattgag tgagaacaga cgtttttgac cttctgttct ttcaggtttg
     3001 atgagttcat gtgtttcata gtaacgtatt tgccttggcg ttaaatccgt taacttactt
     3061 actacactca tagagaagac agccatattt cgtctgattg catcattcga tatcattgtt
     3121 cctctcctct acttttgaac ttgttaataa cttatcataa tttttttagg atttcaaaaa
     3181 tatgtcaggt tttctaacat aaagcttaaa acattgatat aactgcgatt tcgacttcta
     3241 aaacgacata aaacaatcta cagctaaaat attttttcaa tttttaaaaa taatctgata
     3301 aatctccgca aaaaatttca cccaaaacac attatcattt agggtgaaat ttaatctatt
     3361 atataagtcc ttgttctttc aactgattaa cagctcttgt cacagcaatt ttaacgtgtt
     3421 catatgttaa tcctccttga acatatgctt cataaggagg acgaataggt ccatctgcag
     3481 ataattcaat cgatgaacct tgaataaacg taccagctgc cataataaca tcatcttcgt
     3541 aaccaggcat ataactaggt tctggactaa aatgtgcatt aattggggat gcgtgttgaa
     3601 tactttgaca aaatgaaatc atttgttctt tcgtttcaaa tttaactgtt tgaattaaat
     3661 cagttcgttt ttcgtagtac ttcggcgttg tgttcatatt cattttttct aaaaataaac
     3721 tagtaaacaa tgcaccttta agactctgac tgacaacgtg tggtgctaaa aagaaacctt
     3781 gatacatttc aagcaatgca tttaatgatg cacccgcttc tttaccaata ccaggtgctg
     3841 tcaatctata accacatcgt tcaattaaat cttttctacc agcaatgtat ccaccaatct
     3901 tagctaaacc accgccaggg tttttaatta atgatcctgc tattaaatcg gcaccacatt
     3961 ctataggttc acgtctttca acaaattccc cataacagtt atccacaaat attaaaatat
     4021 taggatgcac gtttttcaac ctagtaatta ccttttcaat ttcatctagc ggaattgaag
     4081 gtctttgatc atagcctttc gaacgttgaa tcgctattac tttggtgcgc tcagaaaccc
     4141 catctaacac actttcaata tcgatcttac cttctttaag tgcaatatct ttatacgata
     4201 cgccatgctc cattaaactt tcaataccat ttccgtttac gccaatgact tcaagtaaag
     4261 tgtcatatgg actacccgtt atataaatta attcatcacc atgttttaat aaactttgta
     4321 atgcaatagt aatcgcatgc gtacctgaaa taatttgcgg acgaacaatt gcatcttctg
     4381 ctttaaatgc ctgcgcatat atttcttcta aatgatcacg tccaaagtca tcatacccgt
     4441 atcctgttga tccttgtaga tcactttcgg ttgctttgac atgatgaaat gcatttaata
     4501 ctttttcttg attaatatat gctgtttctt caatttcttt aaaatatggt gctaacgttg
     4561 attcgacgtc agctactatc ttacttatat ctttcatttg aatttcgtcc ttttattatt
     4621 ttttaaatcc ttttatacga taaacatttt caatttcatc atatttaagt tcagtcacta
     4681 atgtatgttg ttttagaaaa tataatcgat ctgcattatt cgtcgcaatt gtctcatcat
     4741 aataagttaa ttgccttttg atttcatcaa taaataacgt cttaacaagt aatttatcac
     4801 catcattttt agaagaaaca aaaacattag gcaaatcact tgctggacga tttgatgcat
     4861 gatcacataa gtccttttta ttaaaaataa ctatttgaga aatatgactc atatctaatt
     4921 gtttgattaa atcattaact gtgtcatact gcgtacggta ttcaggatgg ctactatcta
     4981 cgacatgtac taataaatct gcacctttag cctcttctaa agttgattta aaagctgcaa
     5041 ttaacgtcgt aggtagtttc tgtataaaac caacagtatc tgaaataatt aaattaaatc
     5101 catcatttat ttgaatttgt cgtgttttag gatctaacgt tgcaaataat tgatcttttt
     5161 catacgtctc ttcatttgct aaaacattaa accatgatga tttaccagca tttgtataac
     5221 caactaaagc tacttgaaac acctgatttt gatttctttt atttcgatat ctttcgcgat
     5281 gttcttctac cgtccgcaat tgatgtttaa tttcattcat acgagttcga atatgtctgc
     5341 gatccatctc taacttcgtt tcaccagggc ctctagttcc aataccgcca cctaaacgag
     5401 aaaggctttt accatggcct tgcaatctag gtaataaata atcaagttgt gctagctcta
     5461 cttgcaattt accttcttta cttcttgctc ttaatgcaaa tatttcaaga atcaactgag
     5521 ttctatcaat aatttttaca cctaaagctt catttagtga tttggattgt gcagtcgtta
     5581 attcatcatt tgtgatgact acatcaatat ctttgaactc aataaatgct tgaatttctt
     5641 caattttacc tttaccaaca taatatttgc gatctacacg atctctgttt tgagtaattt
     5701 gacccaacac ttcaagttgg caagtctctg ataaagatga taattcctcc attgtagact
     5761 caaaattaaa ttgcttatca tcttgagcat gtacaccgac taagacagct ttttctagtt
     5821 tatttttagt atcatgaatt tgttgctgag ccatgttata catctcctta ataaaatcct
     5881 actcaaagta ttctatcata gagttttagt aaagactatt acacgatttt gtggtacatt
     5941 ttaagtaaat cgaaaggatg atacaatgga gacaatttat gattttgtag tagaaacaaa
     6001 taaaggtgtt acttacaaat tagatgcata taagggtgac gttatgctaa ttgttaatac
     6061 agctagcgaa tgtggcttta cttcacaatt tgaaggtttg caatcattat acgagaaata
     6121 caaagatcaa gggtttgtaa ttctaggttt cccttgtaat caatttggtg gccaagaacc
     6181 tggttcaggc gaagaagcag ctcaaaactg taaattaaac tatggtgtta ccttccctat
     6241 gcatcaaaaa atagacgtaa aaggggaaca tcaattacct ttatttagat atttgactgc
     6301 ggcacaacac ggattcttta atgagaaaat caaatggaat ttcactaagt ttttagtgga
     6361 tcgcgaaggt aacgttgtta aacgatttgc acctcaaaaa aaacctgttc aaatcgaaag
     6421 agaaatagaa aaattattat aaaatatact agctcatagc aaataaaacg cttcatataa
     6481 agtcgtatca tatattgact cgacctttat atgaagcgtt tttattacaa acattcctct
     6541 attgtatctt gtcatttgaa catcatttaa ttgatgtcac atatgacttt atttctatat
     6601 ctatttatca tacttcaaac gttatgtaca cgagtcatta aatcgtgccc atatgattac
     6661 aacttattct tcactttcag tagatgcttg accttcagtt tctactgtat aagtgctgat
     6721 cgcatgtttg taaatcaagt gttgtttgcc ttgagaattt aagcttacga catacttgtc
     6781 gtattcttca ataacacctt tcatttggaa accgtttaga aagaatacag ttacttcagt
     6841 ttggtttgct ttaaaattct ctagtgcttt gtcttggatg ttttcgtttg caatcatctg
     6901 tcggactcct tttacttaat ctgggttgta atctcatcta acatcatttg aagtgacata
     6961 ttttctttat ctaaccaatg aacactcatt ttattcttga accatgtcat ttgtcgtttt
     7021 gcatattggc gtgaatgttg ctttaaatca ttgacagcat cttcataaat catttgtccg
     7081 ttaatcacag gtattaattc tttatatcca atagcttgca tactttggca agattcatag
     7141 ccttgttcaa caagttgttg cacttctcta aataatccgt gatccaacat aatatcaaca
     7201 cgtttattta ttcttgaata taatgtttta cgcgacattt caatccctaa taataatgta
     7261 tcataatttt cagtaaattg ttgcactttc ttgcgattac tcaaaagttt ttttgttttt
     7321 aaataatatt caatagcgcg caacactctt tggcggttgt tagggtgaat attttctgca
     7381 gaaaccgcat caaattgagc taaataatcg tgtagttgct gattatctaa atgttctaat
     7441 gcagataact tttgtttaac tatggataat tgtgcaggtg taactgtttc atcttctaat
     7501 tcataattat atattaatga ttgaatatat aagcctgttc cacctgctat gattggaact
     7561 ttacctctat tcgttatatc agtaattaaa tcttctgcta atcgcttgaa ttcatatgct
     7621 gaaaatgtat catcaggatt caagatatca attaaatgat gtggaatacc atccatttct
     7681 tcaggtgtta cttttgcagt tccaatattc atatgtttgt agacttgcat agagtcaccg
     7741 cttatgattt caccattgat acgcttcgcg agttcgatgc taagctctgt tttacctgaa
     7801 gcagttggcc ccacaattac tacaataaaa ggcttatttt tattcatatt taattccact
     7861 ttctttattt tttataaaat tgattcaacc atttaaacaa gtcttcccaa attttgtcat
     7921 gatctttttc aaataacact tcatgacgtt tatgatgata aagattcact ttaacatttt
     7981 taatgccagc ttttctatat atattcgcca attttaaaat ccctttacca taatcaccta
     8041 aaggatcgtc atatcctgaa actaataata taggcatggc atgatttaac attttcatat
     8101 tttttaattg tgatgtatgt agcatataat aggctgtttg atataataat tgatttgaca
     8161 cattaaatcc actatatgga tctttaataa agttatctac ttcaattgga ttactagaaa
     8221 tccaatcact ttgtgtacgt aatggacgta tttttttatt aaaactattt gatactaact
     8281 ggttaaccca ttcaactcgt ttctcagcac cataaatttt tgtaatcagt tgtaacactt
     8341 taacggttgg taaacctttc cataatgaat acataccagt accacttaaa attagaccat
     8401 caacatattg cggatatgtt tctacaaaca atctagctat aacggatccc attgaatgtc
     8461 cgattataat gtatggttta tcaacattgc ctctcactgt ttgcgctact tcaaaggcat
     8521 caccgataac tcgtttcata tcatcgtaat gccctcttgt tgattcatca atattaatac
     8581 catgtcctcg atgattatga cgtatcacat cgaagccatg ctttgaaagt gcatgtgcta
     8641 atttatcgta acgttccata tgttcagcca taccatgaaa tatatgaata attccttttg
     8701 aatctacatc tgtaaaattc actttaactt caatattcgt attatcttgt acagttaatt
     8761 taaattgttt ttcagtcatc atctcacacc tttaataaca acgcttttat attcgctttt
     8821 attattatca tacatttagt gatgcatagt ttcaattgac taatgtatct cgtataaaaa
     8881 aactgatgcc cttaaaacag gacaccagtt tatgattgta caaattaatt attctttaac
     8941 tgctggttga ttatttccat gttgcgcttc tttaattgct tgctcaactt cttcagtata
     9001 tgcttcaatt tgagctggag agtaatcaag catatctgcc ataatatcga tgacagcatc
     9061 tttataatct aatacatctt taatattgaa atacatttta ccagaacgac gaactaagaa
     9121 atcgttaggt ttgtatacca tttcttgttg aatactataa acaagttcta cataaatttc
     9181 taatggtaac ttgctatcat ggtattgaga tgtttgcgca atgttgaaca attcatcaac
     9241 atttgaacca tatttagatg ctaaacgtct tgcaacatct tcatcaatgc cgaatccttt
     9301 agctacatct actttttgct ctacaaacgc atcaaagttc ttgctaccac ctacatcgcc
     9361 acctgaaatt gccagacctt ttgtattaca tggactaaat gttaaaccgt agtctttttt
     9421 caagcgttta gatactaaat caacaatgtc ttgagccatg tgacgatagc ctgttaattt
     9481 accacctgca atagttaata aacctgattt accttcccaa atttcatcct tacgagagat
     9541 ttcagaaggg tctttgcctt cttcgtaaat taatggtcta attcctgccc atgttgattc
     9601 aatatcttca tctgtaacat taacactagg gaacatgtaa ttaatcgcat cgattaaata
     9661 gtctctgtct tcttgtgtag ttaatggtga agatttgata ttgtcataga atgtatctgt
     9721 agtacctaca tacgcttttc cttcacgtgg aattgcaaaa atcattcttc catctttttc
     9781 agtatcaaag tatactgctt gacctaatgg gaattttgat tgatcaataa caacatgtac
     9841 acctttagtt aaacgtaatt ttttattatt gcgtgcataa tcaccacttc taacatcatc
     9901 aacccatgga cctgctgcat taaccacttt tttagcctta attgtataat tttcattagt
     9961 taatttatct ataactttaa caccatttac ttgttgattt ttatcataag tgaagtgttc
    10021 agatttagta tagttgataa tttctgcgcc tttttcagca gcacgcttca taacttcaat
    10081 agttaaacgc gcatcgtcag tacgatattc aacatagtaa ccgccgcctt ttagaccttc
    10141 ttttttaact aatggttctt tagctaaagt ttctttttta gataacattt ttttacgttc
    10201 agacttctta acacctgcta aacgatcata cattcctaaa ccaattgatg ttgagaattt
    10261 accaaatgtt ccacctttat gcattggtaa aagcatccac tctggagtcg taacatgagg
    10321 cccattttca taaacaatcg cacgttcttt accagtttcg gcaactactc caatttggaa
    10381 ttgttttaag taacgcaaac caccatggac taattttgta gatcttgagc ttgttccttg
    10441 tgcaaagtct tgcatttcaa ctaatgcaac tttcattcct ctttcactcg cgtctagtgc
    10501 aatacctgca cctgtaatac cgccaccaat aattactaaa tcatattcat catttcttaa
    10561 attcttttta atatgttctc tcttaaaagt agacaatgcc attccaaaac gcctcctaaa
    10621 aaatgtaaaa acgagagact tgtcccacat aaacaatcct tcaattgtac atgttcgaac
    10681 aaatctctcg cttctcgatt caaatattaa cttaaattga ttgtatcaca atgtttatac
    10741 aaaagtctaa tctacaagtt tattctgttt taaaaacttg tgttgcttca acagcttttt
    10801 tccaacctct atataatttt tctctttcgc cttcatccat tttcggatcg aatttttctt
    10861 ctaatttcca gtttttagcg atatcatctt tactctccca gaatccaact gctaaacctg
    10921 ctaaaaatgc agcacctaaa gctgtagttt cttgaatttc aggtctttca acagaagtat
    10981 taacaatgtc tgcttggaac tgcataataa agttattttt aactgcacca ccatcgacac
    11041 gtaaactttg gacatcaata ccagagtctt ttgacattgc ttccataacg tcacgagttt
    11101 ggtaacatag tgattctaaa gttgcacgga taaagtgctc tttttcagtt ccacgtgtta
    11161 aaccgaaaat cgcaccacgt gcttcagaat cccaatatgg tgttcctaaa cctacaaaag
    11221 ctggaacaac ataaacaccc tcagtagagt caactcgtgt cgcataactt tctgattgtg
    11281 gtgctgaatt aatcattctt aatccatcac gtaaccattg gattgctgaa cccgaaacaa
    11341 agatggaacc ttcaagcgca taatttactt ttccatcaat accataagca attgttgtta
    11401 ataaaccact ttcagattta accgctttgt cacctgtatt cattaacatg aagccaccag
    11461 ttccatatgt gtttttcacg tcaccacgtt cgaagcaagc ttgtccaaat aatgctgctt
    11521 gttgatcacc agctactcca gcgattggta cttcttgacc atagaagtgg taatcaattg
    11581 tcttaccata tacttcactc gaagctttaa cttctggcaa catattttta ggtactgtaa
    11641 gtagttctaa taactcatcg tcccattcta aatcatggat attaaacatt aatgtacgac
    11701 tcgcatttga ataatcagta atatgcgcag cttttcctga taatttccat actaaccaag
    11761 tatcaatcgt tccaaataat agatcgccat tttctgcttt ttctcgtgca ccttcaacat
    11821 tgtctagaat ccatttaact tttgtacctg caaaatacgg atctaaaagt aatcctgtct
    11881 tatctctaaa tgtttgttca tatccttgtt gttttaattc tgaacaaatt gattgtgttt
    11941 gacgtgattg ccaaacaatt gcgtgataaa ttgggcggcc agtatgtttg tcccaaacaa
    12001 ccgttgtttc acgttggttt gtaataccga tacctgcaat ttgatcagct ctaacatcat
    12061 tttcattaat tacttccgtc attacagcta acacagatgt ccaaatttca tttgcatcat
    12121 gttcaaccca acctgattgt ggaaaatatt gcttaaactc acgttgtgct acccctgcaa
    12181 tttccccttt ttgattgaat aaaatcgctc ttgagcttgt tgttccttgg tctatagata
    12241 aaatatattt ttccattagt agtatctcct ttattaaaaa ataatcgcta acccattgta
    12301 caaaatctgt taaattttgg cgaaaaaata gcataatcca tgaatcaaac atgttaaatt
    12361 atgctattta cgtttctttt tattttagta aattgattcg atatcacctt tttttgatga
    12421 tttatttaaa atgtaaccta aaatcaaagt aataataact acaacaattg caattgcaca
    12481 accaatatta aatgtatgtt tataaaatac agcgtatacc actgcaccta ataaaccacc
    12541 ggcaattggt cctaagatag gaacgattgc atatgaccaa tttgaaccac ctttaccagc
    12601 tattggtaaa atcgcatgtg caattctcgg acctaaatca cgtgctgggt tgattgcata
    12661 accagtagca ccgcctaaac ttaatccgat tgcaacaatt aatgctccga caattaaagg
    12721 atttaaacca tcggcaattt tgtttacacc gataaataaa atacctaaag ttaatgccat
    12781 tgttccgata atctcactta aaaagttggc aaagtaattc ttaatagccg gtgctgtaga
    12841 gaaaacacct aatttcgcgc cagcttcttc tgtcgctttc caatgtggca agtacattaa
    12901 ccatacaatt gttgctccga caattgcacc taacatttga gcaacaatat aaccaggaac
    12961 taatgaccaa tcaaaacttc cgtctaatgc aagagctaaa gacaccgctg ggtttaaatg
    13021 tgcacctgag aattgaccga cagcaaacac acccattgta accgctaatc cccatccagc
    13081 tgtgatgaca atccaatcag caccattcgc agcacttctc tttaaattga cattggcaca
    13141 aacgccacca ccaaaaagga ttaagattgc agttcctagg aattctgcta aatatacatt
    13201 catattaaat gtcctcccaa aaagacaaag agattcctac actagcagtt tggctcgtag
    13261 aaatctcccg tctctattct atgtattaac ttttattata tttgttatga tactatcgat
    13321 atgaaagcgt tgtcaatggt ttttgtaaaa attttgtcaa atttaatttt tcaagtcttt
    13381 ataaaagtag tttaatttga aaactagaaa taccaatcct aactaattta tttatgacgc
    13441 tcttttgttt aattgacatt cttcagccat attttttatg actagttgca ttttttacta
    13501 ataaacacct ctaaaacttt aatgatttta atcgttttag aggtgctaat tattttattt
    13561 gattattttt tgtttgatac ctactgcata tcccatatga aaacggcttt tttattatgt
    13621 tatatgacta aatctcgtga aaaatgaaat ttttgcagac ttatgattta ccaaagttta
    13681 tcataactag tagttacata tttcgctcca tttttaacag cttcattgac ttcatctatt
    13741 gtattaatta ggccacctgc aatgacttgt gtgtttgttt ctttctgaat atgatgaatc
    13801 gctttactcg caacacctgg aagtacttca acaaaatcag gttcaacttt ttttatcaaa
    13861 tctatactgc gtttcaatgc ttgactatca ataataaata ctctaaaaat
//
LOCUS       DANJFZ010000065        13871 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.065, whole genome shotgun sequence.
ACCESSION   DANJFZ010000065 DANJFZ010000000
VERSION     DANJFZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.065"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            310..1152
                     /locus_tag="PWN45_001709"
     CDS             310..1152
                     /locus_tag="PWN45_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0623478.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(1277..1588)
                     /locus_tag="PWN45_001710"
     CDS             complement(1277..1588)
                     /locus_tag="PWN45_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0623479.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1675..2751
                     /locus_tag="PWN45_001711"
     CDS             1675..2751
                     /locus_tag="PWN45_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0623480.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2816..3127
                     /locus_tag="PWN45_001712"
     CDS             2816..3127
                     /locus_tag="PWN45_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0623481.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3227..4084
                     /locus_tag="PWN45_001713"
     CDS             3227..4084
                     /locus_tag="PWN45_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDL0623482.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            4113..4709
                     /locus_tag="PWN45_001714"
     CDS             4113..4709
                     /locus_tag="PWN45_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL0623483.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4730..8554
                     /locus_tag="PWN45_001715"
     CDS             4730..8554
                     /locus_tag="PWN45_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0623484.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            8578..9891
                     /locus_tag="PWN45_001716"
     CDS             8578..9891
                     /locus_tag="PWN45_001716"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL0623485.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9965..10456
                     /locus_tag="PWN45_001717"
     CDS             9965..10456
                     /locus_tag="PWN45_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL0623486.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            10530..11888
                     /locus_tag="PWN45_001718"
     CDS             10530..11888
                     /locus_tag="PWN45_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623487.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            12023..12127
                     /locus_tag="PWN45_001719"
     CDS             12023..12127
                     /locus_tag="PWN45_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623488.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            12588..13679
                     /locus_tag="PWN45_001720"
     CDS             12588..13679
                     /locus_tag="PWN45_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDL0623489.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         5397 a         1885 c         2553 g         4036 t
ORIGIN      
        1 ctctataatt aaaagtgact aaacaaataa ctaaaggaga taatgcgcct atgaacaatg
       61 ttatactaaa tacctttgat ttaaaagaat aaagcttgca ttttacttgt aaattttaat
      121 tattaattta aaaatacagg ccgatggtta tgatgcattt acatataata tgattgcatt
      181 aatctttata agtagttaga agtgtattag tgtggggcgt ttcgtttttg ttcgcattta
      241 tgaatagtga tatgatgtaa taaacacatg ctaattgtaa taacgaatac agaaagaata
      301 ggtgatgtta tgaaaatcgg ggatatatct attcattatc taaatggtgg caatacaaaa
      361 atggatggcg gtgcaatgtt tggtgttgtt ccgaagccgt tgtggtcaaa gcaatacaat
      421 gcaaatgaac gaaatcaaat caatttaccg acacatccaa ttttgattca aacggctcaa
      481 tataatttga ttatagatgc aggtattggt aatggtaaat tatctgaaaa gcaattacgt
      541 aattttggag tagatgaaga aagtcatata attgctgatt tggcaaatta taatttaacg
      601 ccaaaggata ttgattatgt gctaatgaca catatgcatt ttgatcatgc tgccggtttg
      661 actgatcaag caggacatgc aatttttgaa aatgcgattc atgttgtgca acaagatgag
      721 tggcatgagt ttattgcacc taatataaga agtaaatcaa cttactggga taagaataaa
      781 ggcgattata gtaacaagtt gattttattc gaaaaacatt ttgaaccggt tctgggtatc
      841 aagatgcaac atagtggagg tcatagcttt ggccacacga ttattacgat tgaaagtcaa
      901 ggagataaag cagttcatat gggtgatata ttcccaacta ctgcacataa aaatcctcta
      961 tgggtaacgg catatgatga ttatcctatg caatcgattc gtgaaaaaga acgcatgata
     1021 ccatatttta ttcagcaaca atattggttc ttgttttatc atgatgaaaa ctactttgct
     1081 gtaaaataca gcgataatgg tgaaaacata gatgcatata ttttacgtga aacattagtt
     1141 gataataact aaaataaaga tgtattacta aacaaatttt caaaaataaa aaattgagcc
     1201 acatccaatc ttactaatta gggtgtggct catttttaag ttttacgatc caaatcaaat
     1261 atggataaaa ttcgtattaa cgctctacaa tgttaatgac ttcaccagta tatgcatctg
     1321 cataaaaatc ataatgaata ttttgaccat ttttaatagt tgtaattcca ccttgataaa
     1381 ctaaacggta tttatcagtt tcaggatgaa cgaatggttc ataaacgata tatgagcctt
     1441 tgacattcat aaaatatgat tttacttcat taagtacttt aacgggatta tagcgtttac
     1501 gattaataaa ttggatgatt gaaatggtag aaataattac aatggctact gcaattattg
     1561 ttggaattat atatttcagt ttagtcatca tttatgctcc cttgatttca aattcatatc
     1621 attagtttac catattgaag atgatataat aacttataag gagtgagaac ttttatgaac
     1681 ataaataaaa aagtaacatt acaacgaatt caaactttaa ctgagcttca tggggcacca
     1741 ggttttgaag aagaagtaaa aaattatatg actcagcaaa tggcgccgta cgtagatgaa
     1801 tttattgaaa atcgtatggg tggatttttt ggtgtgaaaa aatctaaaaa tccaaatgca
     1861 aaacgtgtaa tgattgcagc acatatggat gaaatcggat ttatgattac aaatatcact
     1921 aaaaatggaa tgattcaatt cacaaattta ggtggtgttg caaatgatat ttggcaagga
     1981 caacgcttag taattaaaaa tagaaatggc gataaaatta tcggtgttgt ttctaatata
     2041 cctaaacatt ttcgtactgg tagtgaaggt gcaccggaaa ttaaagattt aacattagat
     2101 ataggtgctc aaaatgaaga tgaggtgcgt gagcgcggaa tagatatagg agatacaatt
     2161 gtacctcaca cgccattcac acagttatct gaacatcgat atagtgctaa agcatgggat
     2221 aatcgttatg gttgtgtctt ggcaattgaa atactagaat tattaaaaga tatagaatta
     2281 gatgtagact tgtatgttgg cgcaaatgtt caagaagagg ttggattacg aggtgcgaaa
     2341 gcatctgcag agatgataga cccagacgtt gcatttgtag ttgattgttc acctgccaat
     2401 gacgttaaag gaaaccaacc attatctggt gaacttggta aagggacgtt aattcgcata
     2461 aaagacggta caatgatttt aaagcctgta tttagagact atttattaaa gttagtagaa
     2521 gcacatgaca ttgaacatca atactatatg tcaccaggtg gaacagatgg tggagaaatt
     2581 cataaagcta atattggtat tccgactgca gttattggtg tatgtgcacg atatattcat
     2641 agtacagact cagtatttga tataagagac tattttgcag ctagatcttt actttcagaa
     2701 gccatttgta atttagataa taatcaaata gaaacattac aatataaata atcgggtaat
     2761 aacaactatt atctctaaat agttatatat aatcattaat taaggagaca taaaaatgaa
     2821 acaacttgaa tcagaacaac aatttgaatc tttaaaacaa ggtgctacag tatttgaatt
     2881 cactgcaggc tggtgtccag attgtagagt gatagaacca gatttaccgg aattagaagc
     2941 gagatatcct atgtttgact tcgtatcagt agaccgtgat aaatttatgg atatttgtat
     3001 tgaaaatggt attatgggta ttccaagttt tctagtatat aaaaatggag aactgcttgg
     3061 aagttatatt ggaaaagaac gaaaatcaat tgaacagata gatgcatttt tagctcaata
     3121 cgtgtaattt agactagaga aaaacggggt aatactcgtt tttctctgtt actatgtgtt
     3181 gatttattgt aaactattat aaggtgcgaa attaggagtg ttacatatga atacctttca
     3241 aatgagagat aaattaaagg aacgtttaag ccatttagac gttgatttta aatttaatcg
     3301 tgaagaagaa actttgcgta tttatcgaac agataataac aaaggtatca cgattaaact
     3361 taacgctata gtcgcaaaat atgaagataa aaaagaaaaa attgtagatg aaattgttta
     3421 ttacgttgat gaagctattg cacaaatggc agataaaacc cttgagagta tatcatctag
     3481 tcaaattatg cctgtcatta gagcgactag ctttgataaa aaaactaaac aaggtgttcc
     3541 ttttatctat gatgagcata ctgcagaaac agcagtttat tatgcagtcg atttagggaa
     3601 atcatatcgt ctaattgacg aaagcatgtt agaggatttg aagttaactg aacaacaaat
     3661 aagagaaatg tctctgttta atgttagaaa attgtcaaat tcatatacga ctgatgaagt
     3721 aaaaggtaat attttttatt ttattaactc aaatgacggg tatgatgcaa gtaggatact
     3781 aaatactgca tttttaaatg aaattgaggc acaatgtcaa ggcgaaatgc tcgtagcagt
     3841 gccacaccaa gatgtgttaa ttattgcaga tatacgcaat aaaacaggat atgatgtgat
     3901 ggcacattta acaatggaat ttttcactaa aggtctagtt ccaattacat cattatcctt
     3961 tggatataaa cagggtcatc ttgaaccgat atttatttta ggtaaaaata ataaacaaaa
     4021 aagagatcca aacgtgattc agcgtttaga agcaaatcgt cgtaaattta ataaagataa
     4081 atagaaataa ttggataagg agttttgtca taatgaattt attttacaat cctaaatatg
     4141 taggagatgt cgcattttta caaattgaac cagttgaagg tgaattaaac tacaataaaa
     4201 aaggtaatgt tgttgaaatt actaatgaag gtaatgttgt aggttataat atttttgaaa
     4261 tttcaaaaga tataacaatt gaagaaaaag gtcatattaa attaactgat gaacttgtaa
     4321 atgtattcca aaagcgtatt tcagaagctg gttttgatta taaattaaat gctgatctat
     4381 caccgaaatt tgtagttggc tacgttgaaa ctaaagacaa acatcctgat gcagataaat
     4441 taagtgtact aaatgtaaac gttggaaatg acacattaca aattgtatgt ggcgcgccta
     4501 acgttgaagc tggacagaaa gttgttgttg ctaaagtagg tgcagtgatg cctagcggta
     4561 tggtaattaa agatgctgaa ttacgtggtg ttgcctcaag cggtatgatt tgttcaatga
     4621 aagaattgaa tttacctaat gcacctgaag aaaaaggtat tatggtatta aatgacagct
     4681 atgaaattgg acaagcattt tttgaataat taaggaaggt agtgaaaata tgagctggtt
     4741 tgataaatta ttcggcgaag ataatgattc aaatgatgac ttgattcata gaaagaaaaa
     4801 aagacgtcaa gaatcacaaa atatagataa cgatcatgac tcattactgc ctcaaaataa
     4861 tgatatttat agtcgtccga ggggaaaatt ccgttttcct atgagcgtag cttatgaaaa
     4921 tgaaaatgtt gaacaatctg cagatactat ttcagatgaa aaagaacaat accatcgaga
     4981 ctatcgcaaa caaagccacg attctcgttc acaaaaacga catcgccgta gaagaaatca
     5041 aacaactgaa gaacaaaatt atagtgaaaa acgtgggaat tctaaaatat cacagcaaag
     5101 tataaaatat aaagatcatt cacattacca tacgaataag ccaggtacat atgtttctgc
     5161 aattaatggt attgagaagg aaacgcacaa gccaaaaaca cataatatgt attctaataa
     5221 tacaaatcat cgtgctaaag attcaactcc agattatcac aaagaaagtt tcaagacttc
     5281 agaggtaccg tcagctattt ttggcacaat gaaacctaaa aagttagaaa atggtcgtat
     5341 ccctgtaagt aaaccttcag aaaaagttga gtcagataaa caaaaatatg ataaatatgt
     5401 agctaagacg caaacgtctc aaaataaaca attagaacaa gaaaaacaaa atgatagtgt
     5461 tgtcaaacaa ggaactgcat ctaaatcatc tgatgaaaat gtatcatcaa caacaaaatc
     5521 aatgcctaat tattcaaaag ttgataatac tatcaaaatt gaaaatattt atgcttcaca
     5581 aattgttgaa gaaattagac gtgaacgaga acgtaaagtg cttcaaaagc gtcgatttaa
     5641 aaaagcgttg caacaaaagc gtgaagaaca taaaaacgaa gagcaagatg caatacaacg
     5701 tgcaattgat gaaatgtatg ctaaacaagc ggaacgctat gttggtgata gttcattaaa
     5761 tgatgatagt gacttaacag ataatagtac agatgctagt cagcttcata caaatggcat
     5821 agagaatgaa actgtatcaa atgatgaaaa taaacaagcg tcaatacaaa atgaagacac
     5881 taatgacact catgtagatg aaagtccata caattatgag gaagttagtt tgaatcaagt
     5941 atcgacaaca aaacaattgt cagatgatga agttacggtt tcgaatgtaa cgtctcaaca
     6001 tcaatcagca ctacaacata acgttgaagt aaatgataaa gatgaactaa aaaatcaatc
     6061 cagattaatt gctgattcag aagaagatgg agcaacgaat aaagaagaat attcaggaag
     6121 tcaaatcgat gatgcagaat tttatgaatt aaatgataca gaagtagatg aggatactac
     6181 ttcaaatatc gaagataata ccaatagaaa cgcgtctgaa atgcatgtag acgctcctaa
     6241 aacgcaagag tacgcagtaa ctgaatctca agtaaataat atcgataaaa cggttgataa
     6301 tgaaattgaa ttagcaccgc gtcataaaaa agatgaccaa acaaacttaa gtgtcaactc
     6361 attgaaaacg aatgatgtga atgataatca tgttgtggaa gattcaagca tgaatgaaat
     6421 agaaaagaat aacgcagaaa ttacagaaaa tgtgcaaaac gaagcagctg aaagtgaaca
     6481 aaatgtcgaa gagaaaacta ttgaaaacgt aaatccaaag aaacagactg aaaaggtttc
     6541 aactttaagt aaaagaccat ttaatgttgt catgacgcca tctgataaaa agcgtatgat
     6601 ggatcgtaaa aagcattcaa aagtcaatgt gcctgaatta aagcctgtac aaagtaagca
     6661 agctgtgagt gaaagaatgc ctgcgagtca agccacacca tcatcaagat ctgattcaca
     6721 agagtcaaat acaaatgcat ataaaacaaa taatatgaca tcaaacaatg ttgagaacaa
     6781 tcaacttatt ggtcatgcag aaacagaaaa tgattatcaa aatgcacaac aatattcaga
     6841 gcagaaacct tctgttgatt caactcaaac ggaaatattt gaagaaagtc aagatgataa
     6901 tcaattggaa aatgagcaag ttgatcaatc aacttcgtct tcagtttcag aagtaagcga
     6961 cataactgaa gaaagcgaag aaacaacaca tccaaacaat actagtggac aacaagataa
     7021 tgatgatcaa caaaaagatt tacagtcatc attttcaaat aaaaatgaag atacagctaa
     7081 tgaaaataga cctcggacga accaacaaga tgttgcaaca aatcaagctg tacaaacatc
     7141 taagccgatg attcgtaaag gcccaaatat taaattgcca agtgtttcat tactagaaga
     7201 accacaagtt attgagtcgg acgaggactg gattacagat aaaaagaaag aactgaatga
     7261 cgcattattt tactttaatg tacctgcaga agtacaagat gtaactgaag gtccaagtgt
     7321 tacaagattt gaattatcag ttgaaaaagg tgttaaagtt tcaagaatta cggcattaca
     7381 agatgacatt aaaatggcat tggcagcgaa agatattcgt atagaagcgc ctattccagg
     7441 aactagtcgt gttggtattg aagttccgaa ccaaaatcca acgacagtca acttacgttc
     7501 tattattgaa tctccaagtt ttaaaaatgc tgaatctaaa ttaacagttg cgatggggta
     7561 tagaattaat aatgaaccat tacttatgga tattgctaaa acgccacacg cactaattgc
     7621 aggtgcaact ggatcaggga aatcagtttg tatcaatagt attttgatgt ctttactata
     7681 taaaaatcat cctgaggaat taagattatt acttatcgat ccaaaaatgg ttgaattagc
     7741 tccttataat ggtttgccac atttagttgc accggtaatt acagatgtca aagcagctac
     7801 acagagttta aaatgggccg tagaagaaat ggaacgacgt tataagttat ttgcacatta
     7861 ccatgtacgt aatataacag catttaacaa aaaagcacca tatgatgaaa gaatgccaaa
     7921 aattgtcatt gtaattgatg agttggctga tttaatgatg atggctccgc aagaagttga
     7981 acagtctatt gctagaattg ctcaaaaagc gagagcatgt ggtattcata tgttagtagc
     8041 tacgcaaaga ccatctgtca atgtaattac aggtttaatt aaagccaaca taccaacaag
     8101 aattgcattt atggtatcat caagtgtaga ttcgagaacg atattagaca gtggtggagc
     8161 agaacgcttg ttaggatatg gcgatatgtt atatcttggt agcggtatga ataaaccgat
     8221 tagagttcaa ggtacatttg tttctgatga cgaaattgat gatgttgttg attttatcaa
     8281 acaacaaaga gaaccggact atctatttga agaaaaagaa ttgttgaaaa aaacacaaac
     8341 acaatcacaa gatgaattat ttgatgatgt ttgtgcattt atggttaatg aaggacatat
     8401 ttcaacatca ttaatccaaa gacatttcca aattggctat aatagagcag caagaattat
     8461 cgatcaatta gagcaactcg gttatgtttc gagtgctaat ggttcaaaac caagggatgt
     8521 ttatgttacg gaagcagatt taaataaaga ataattatga gtaaggagtt ttatataatg
     8581 acacactatc attttgtcgg aattaaaggt tctggcatga gttcattagc acaaatcatg
     8641 catgatttag gacatgaagt tcaaggatcg gatattgaga actacgtatt tacagaagtt
     8701 gctcttagaa ataaggggat aaaaatatta ccatttgatg ctaataacat aaaagaagat
     8761 atggtagtta tacaaggtaa tgcattcgcg agtagccatg aagaaatagt acgtgcacat
     8821 caattgaaat tagatgttgt aagttataat gattttttag gacagattat tgatcaatat
     8881 acttcagtag ctgtaactgg tgcacatggt aaaacttcta caacaggttt attatcacat
     8941 gttatgaatg gtgataaaaa gacttcattt ttaattggtg atggcacagg tatgggattg
     9001 cctgaaagtg attatttcgc ttttgaggca tgtgaatata gacgtcactt tttaagttat
     9061 aaacctgatt acgcaattat gacaaatatt gatttcgatc atcctgatta ttttaaagat
     9121 attaatgatg tttttgatgc attccaagaa atggcacata atgttaaaaa aggtattatt
     9181 gcttggggtg atgatgaaca tctacgtaaa attgaagcag atgttccaat ttattattat
     9241 ggatttaaag attcggatga catttatgct caaaatattc aaattacgga taaaggtact
     9301 gcttttgatg tgtatgtgga tggtgagttt tatgatcact tcctgtctcc acaatatggt
     9361 gaccatacag ttttaaatgc attagctgta attgcgatta gttatttaga gaagctagat
     9421 gttacaaata ttaaagaagc attagaaacg tttggtggtg ttaaacgtcg tttcaatgaa
     9481 actacaattg caaatcaagt tattgtagat gattatgcac accatccaag agaaattagt
     9541 gctacaattg aaacagcacg aaagaaatat ccacataaag aagttgttgc agtatttcaa
     9601 ccacacactt tctctagaac acaggcattt ttaaatgaat ttgcagaaag tttaagtaaa
     9661 gcagatcgtg tattcttatg tgaaattttt ggatcaatta gagaaaatac tggcgcatta
     9721 acgatacaag atttaattga taaaattgaa ggtgcatcgt taattaatga agattctatt
     9781 aatgtattag aacaatttga taatgctgtt attttattta tgggtgcagg tgatattcaa
     9841 aaattacaaa atgcatattt agataaatta ggcatgaaaa atgcgtttta atatgtttat
     9901 aatagagtag tatgggtatt tattattaat gacattatta catgttaatt aggaggcgtt
     9961 tttaatggat tggattttac caattgctgg aattatcgct gcgattgcat tcttaatttt
    10021 atgtatcggt atcgtagctg tattaaattc tgttaagaaa aacttagatt atgttgcaaa
    10081 aacacttgac ggtgtagaag gtcaagttca aggtattact cgtgaaacaa cagatttact
    10141 tcataaagta aaccgtttaa ctgaggatat ccaaggtaaa gtagatcgtt taaactcagt
    10201 tgtagatgct gttaaaggta tcggtgactc agtacaaacg ttaaacagct ctgtagatcg
    10261 tgtaacaaat tcaattacac ataatatttc tcaaaatgaa gataaaatct cacaagttgt
    10321 tcaatggtca aatgttgcaa tggaaattgc agacaaatgg caaaatagac actaccgtcg
    10381 tggaagtgca aattacaaag ctaataatgt agcaactgat gcaaatcata gctatacttc
    10441 tagagtagat aaataattaa taattaaaca aatgcactta aatgaggttg ttacttattt
    10501 aagtgcattt gttataggag gatatacaaa tgacgcagca acaaaatgat aaaagaacat
    10561 taaaaaataa acacacttat caaaatgaac cactaccaaa ccgtaaagat tttgttgtta
    10621 gttttataac tggcgcgctt gttggttcag ctttaggttt gtattttaaa aataaagttt
    10681 atcaaaaagc agatgattta aaagtcaaag aacaagaact gtcgcaaaag tttgaagaaa
    10741 gaaaaacgca acttgaagaa acggttgcct atacaaaaga acgtgttgaa ggatttttaa
    10801 acaaatctaa aaatgagcaa gcggcattaa aggcacaaca agcagcgata aaagaagaag
    10861 caagtgcaaa taatttaagt gatacatcac aagaagcaca agagattcaa gaagctaaaa
    10921 gagaagcaca agcagaagcg gataaaagtg tggctgtatc aaataaagaa tcaaaagcag
    10981 tggcattgaa agcacaacaa gcagcgataa aagaagaagc aagtgcaaat aatttgagtg
    11041 atacatcaca agaggcacaa gagattcaag aagctaaaaa agaagcacaa gcagaaacag
    11101 ataaaagtgc agctgtatca aatgaagaac caaaagcagt ggcattgaaa gcacaacaag
    11161 cagcgataaa agaagaagca agtgcaaata atttaagtga tatatcacaa gaggcacaag
    11221 aggttcaaga agctaaaaaa gaagcacaag cagagaaaga cagtgacaca ttaactaaag
    11281 atgcaagtgc agcaaaggta gaagtatcaa aaccagagtc acaagctgaa agattagcaa
    11341 acgctgcaaa acagaagcaa gctaaattaa caccaggttc aaaagagagt caattaactg
    11401 aagcgttatt tgcagaaaaa ccagttgcta aaaatgactt gaaagaaatt cctcaattag
    11461 ttactaaaaa gaatgatgta tcagagacag agacggttaa tatagataat aaagacactg
    11521 ttaaacaaaa agaagctaaa tttgaaaatg gtgttattac acgtaaagct gatgaaaaaa
    11581 caactaataa tacagctgtt gacaagaaat caggtaaaca atctaaaaaa acaacacctt
    11641 caaataaacg aaatgcatca aaagcatcta caaataaaac ttcaggtcag aaaaagcaac
    11701 ataataagaa atcatcacaa ggtgcaaaga aacaaagtag ttcaagtaag tcaactcaaa
    11761 agaataatca aactagtaat aagaattcaa aaacaacaaa tgctaaatca tccaatgcat
    11821 caaaaacgcc aaatgctaaa gttgagaaag ctaaaagtaa aatagagaaa cgtacattca
    11881 atgactaaga ttactaaaat cgttgaataa tcaatcatag cagtaagcta tgtaatatat
    11941 ttttaaaaat tgaatttcaa aataaaaggt aggcattcag gtgcttacct tttttgatat
    12001 catcagttta ttttgaaagg caatgcgatc atttcatgta tttatgttgt ttgaaacatc
    12061 gttagataac aatagtgata ttgcacattt taagctgaag atggaaaaat attcgataat
    12121 caaataaaca atgaatttta gaaggtacaa tgacgttcac taatttaaat atagctgaat
    12181 gtgttggtga gtgatgttca ctatagattt atattaatat acaaagacaa aggttgttaa
    12241 tttttattaa gcgttaggtt gaatgtatga gaattttaga tttataatag aagatagaaa
    12301 cgaaaatttt tcttaaaagc agtaatgttg actcaaaata agctataata atgacactta
    12361 tttaattgat taacatttgc taataaatat caatatagaa tataactttc caataatgac
    12421 tgagaaaatc gaaatgtcag tctcgaatca tataattaga aaattgatta ttttctgtca
    12481 atttagggtt gaactataca tatgatattg ttagaatatt ttttaacatt atattttatt
    12541 gctttaaagt ggaatatact tgaaataatt agtagaggtg agtaaggatg agtaataaat
    12601 tagaatcata cagaagtgag attgtatcac tgaatcatca aattttagac ttattatcta
    12661 aacgtggtga actagcacaa aaaattgggg aagaaaaatt aaaacaaggt acacgtattt
    12721 atgatccaca acgtgaaaaa gaaatgctta acgacttaat cgatagtaac aaaggaccat
    12781 tcaacgataa tactattaag caattattta aagaaatttt caaagcctct acagatttac
    12841 aaaaatctga aaatgaaaaa catttatatg tatcacgtaa gttgaaacct gaagatacga
    12901 ttgtaacatt tgataatggg ggcattattg gagacggcaa taaatcattt gtatttgggc
    12961 catgttcagt tgaatcattt gaacaagttg aagctgttgc taaaaactta catgctaaag
    13021 gtgaaaaatt tattcgtggc ggtgcattta aaccacgtac atcaccatat gatttccaag
    13081 gcctaggtgt tgaaggactt aaaatactta aacagattaa agataaatat gatttaaatg
    13141 ttgtcagcga aatcgtaaat ccaaatgatt ttgaagtggc tgatgagtat ttagacgtat
    13201 tccaaattgg tgcacgtaat atgcaaaact tcgagttatt aaaagaagct ggccgtacga
    13261 aaaagcctat tctattaaaa cgtggtttat ctgctacaat cgaagagttt gtttatgcag
    13321 ctgaatacat tgcttcacaa ggtaatcaaa acattatttt atgtgaacgt ggaatccgaa
    13381 cttatgaaaa ggcgacacgt aacactttag atatttcagc agtaccaatt ttaaaacaag
    13441 gtacacactt accagtcatg gtagatgtta cgcatagtac aggtcgtaaa gatatcatgt
    13501 taccaactgc gaaagcagca ttagcagttg gtgctgatgg agttatggct gaggtgcatc
    13561 cagatccatc tgttgcactt agtgatgcgg gtcaacaaat ggatttagat gaattccaag
    13621 cattttatga tgaattaaag cctttagctg atttatataa cgctaaaaag ttaaaataat
    13681 attccaagga aactatagac tacttaacta atatgtcatg ttgaagtaga atattatctt
    13741 tgaatcgaca attttaaact tacagccatt ctaagagtat attactttta gagtggctat
    13801 tattttttgt atagaaataa aggtatactg cacttaacga ttgttataat acttcgacac
    13861 ttgttcaatt t
//
LOCUS       DANJFZ010000066        13687 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.066, whole genome shotgun sequence.
ACCESSION   DANJFZ010000066 DANJFZ010000000
VERSION     DANJFZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.066"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            45..1016
                     /gene="ribF"
                     /locus_tag="PWN45_001721"
     CDS             45..1016
                     /gene="ribF"
                     /locus_tag="PWN45_001721"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL0623490.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            1131..1400
                     /gene="rpsO"
                     /locus_tag="PWN45_001722"
     CDS             1131..1400
                     /gene="rpsO"
                     /locus_tag="PWN45_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL0623491.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            1770..3866
                     /gene="pnp"
                     /locus_tag="PWN45_001723"
     CDS             1770..3866
                     /gene="pnp"
                     /locus_tag="PWN45_001723"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL0623492.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            4102..5775
                     /locus_tag="PWN45_001724"
     CDS             4102..5775
                     /locus_tag="PWN45_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0623493.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            6031..8400
                     /locus_tag="PWN45_001725"
     CDS             6031..8400
                     /locus_tag="PWN45_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0623494.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            8405..9118
                     /locus_tag="PWN45_001726"
     CDS             8405..9118
                     /locus_tag="PWN45_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0623495.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            9149..10414
                     /locus_tag="PWN45_001727"
     CDS             9149..10414
                     /locus_tag="PWN45_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0623496.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            10414..11700
                     /locus_tag="PWN45_001728"
     CDS             10414..11700
                     /locus_tag="PWN45_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0623497.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            11700..12404
                     /locus_tag="PWN45_001729"
     CDS             11700..12404
                     /locus_tag="PWN45_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0623498.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            12509..13336
                     /locus_tag="PWN45_001730"
     CDS             12509..13336
                     /locus_tag="PWN45_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDL0623499.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            13355..>13687
                     /locus_tag="PWN45_001731"
     CDS             13355..>13687
                     /locus_tag="PWN45_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0623500.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVI"
BASE COUNT         5022 a         1866 c         2630 g         4169 t
ORIGIN      
        1 gaaattaaac ctaaaaaagt ctttaattaa aggagataga atttatgaaa gtcatagaag
       61 tgacacatcc tatacaatct aaacagtata ttacagagga tgttgcaatg gcattcggat
      121 ttttcgatgg catgcataaa ggtcatgaca aagtctttga tatattaaac gaaatagctg
      181 aggcacgcag tttaaaaaaa gcggtgatga catttgatcc gcatccgtct gtcgtgttga
      241 atcctaaaag aaaacgaaca acgtatttaa cgccactttc agataaaatc gaaaaaatta
      301 gccaacatga tattgattat tgtatagtgg ttaatttttc atctaggttt gctaatgtga
      361 gcgtagaaga ttttgttgaa aattatataa ttaaaaataa tgtaaaagaa gtcattgctg
      421 gttttgattt tacttttggt aaatttggaa aaggtaatat gactgtactt caagaatatg
      481 atgcgtttaa tacgacaatt gtgagtaaac aagaaattga aaatgaaaaa atttctacaa
      541 cttctattcg tcaagattta atcaatggtg agttgcaaaa agcgaatgat gctttaggct
      601 atatatattc tattaaaggc actgtagtgc aaggtgaaaa aaggggaaga actattggct
      661 tcccaacagc taacattcaa cctagtgatg attatttgtt acctcgtaaa ggtgtttatg
      721 ctgttagtat tgaaatcggc actgaaaata aattatatcg aggggtagct aacataggtg
      781 taaagccaac atttcatgat cctaacaaag cagaagttgt catcgaagtg aatatctttg
      841 actttgagga taatatttat ggtgaacgag tgaccgtgaa ttggcatcat ttcttacgtc
      901 ctgagattaa atttgatggt atcgacccat tagttaaaca aatgaacgat gataaatcgc
      961 gtgctaaata tttattagca gttgattttg gtgatgaagt agcttataat atctagagtt
     1021 gcgtatagtt atataaacaa tctataccac acctttttct tagtaggtcg aatctccaac
     1081 gcctaactcg gattaaggag tattcaaaca ttttaaggag gaaattgatt atggcaattt
     1141 cacaagaacg taaaaacgaa atcattaaag aataccgtgt acacgaaact gatactggtt
     1201 caccagaagt acaaatcgct gtacttactg cagaaatcaa cgcagtaaac gaacacttac
     1261 gtacacacaa aaaagaccac cattcacgtc gtggattatt aaaaatggta ggtcgtcgta
     1321 gacatttatt aaactactta cgtagtaaag atattcaacg ttaccgtgaa ttaattaaat
     1381 cacttggtat ccgtcgttaa tcttaatata acgtctttga ggttggggca tatttatgtt
     1441 ccaaccttaa tttatattaa aaaagctttt tacaaatatt aacatttatt atatgttaag
     1501 ctaatattga gtgaataata aggttacaat gagataaaga tgatataagt acacctagag
     1561 taataatcaa gatattaaaa ataaagtatg tttttttaaa aaatataact tatatttata
     1621 ctgataaggg tgggacgata agtctatttt gtaaataata gatggatatc ccgctctctt
     1681 tttttccaat tcaatatttt ataactaata ttaaaatacg ataataaatg atatgatata
     1741 actattagat tcaagagagg agatttataa tgtctcaaga aaagaaagtt tttaaaactg
     1801 aatgggcagg aagatcttta acgattgaaa cagggcaatt agctaaacaa gcaaatggcg
     1861 ctgtattggt tcgttatgga gatacagtcg tgttatcgac ggcaactgca tcaaaagaac
     1921 ctcgtgatgg agatttcttc ccattaacag tgaactatga agaaaaaatg tacgctgcgg
     1981 gtaaaattcc tggtggattt aaaaagagag aaggacgtcc tggtgacgat gcaacattaa
     2041 ctgcgcgatt aattgataga ccaattagac ctttattccc taaaggatat aagcatgatg
     2101 ttcaaattat gaacatggta ttaagtgcag atcctgattg ttcaccacaa atggctgcaa
     2161 tgattggttc atctatggcg cttagtgtgt cggatattcc attccaaggg ccaatcgccg
     2221 gtgtaaatgt gggttatatt gacggtaaat atatcattaa cccaacagta gaagaaaaag
     2281 aagtttctcg tttagacctt gaagtagctg gtcataaaga tgcggtaaac atggtagagg
     2341 caggcgctag tgagattact gaacaagaaa tgttagaggc gattttcttt ggtcatgaag
     2401 agattcaacg tttagttgat ttccaacaac aaatcgtcga ccacattcaa cctgttaaac
     2461 aagaatttat tccagcagag cgtgatgaag cgctagttga acgtgtaaaa tctttaaccg
     2521 aagaaaaagg acttaaagaa acagttttaa catttgataa acaacaacga gatgaaaatc
     2581 ttgataactt aaaagaagaa atcgtcaatg aatttatcga tgaagaagat ccagagaatg
     2641 aattacttat taaagaagtt tatgcaattt taaatgaatt agtgaaagaa gaagttcgac
     2701 gtttaattgc agatgaaaaa attagaccag acggccgtaa acctgatgaa atccgtccat
     2761 tagattctga agttggtatt ttacctagaa cgcatggttc aggtctattt acacgtggtc
     2821 agactcaagc actttcagtt ttaacattag gtgctttagg cgattatcaa ttaattgatg
     2881 gtttaggacc tgaagaagaa aaaagattca tgcatcatta caacttcccg aatttttcag
     2941 taggtgaaac tggtccagta cgtgcgccag gtcgtcgtga aattggacat ggtgcgttag
     3001 gtgaaagagc attaaaatat attattcctg atactgctga tttcccatat acaattcgta
     3061 ttgtaagtga ggtacttgaa tcaaatggtt catcatctca agcgtcaatt tgtggatcaa
     3121 cattagcatt aatggatgcg ggcgtaccga ttaaagcacc agttgctggt attgctatgg
     3181 gccttgttac acgtgaagat agctatacga ttttaactga tatccaaggt atggaagatg
     3241 cattaggtga tatggacttt aaagtcgctg gtactaaaga aggtattaca gcaatccaaa
     3301 tggatattaa aattgacggt ttaacgcgtg aaattatcga agaggctcta gaacaagcga
     3361 gacgtggtcg tttagaaata atgaatcata tgttacaaac aattgatcaa ccacgtactg
     3421 aattaagtgc ttacgcgcca aaagttgtaa ctatgacaat taaaccagat aagattagag
     3481 atgttatcgg acctggtggt aaaaaaatta acgaaattat tgatgaaaca ggtgttaaat
     3541 tagatattga acaagatggt actatcttta ttggtgctgt tgatcaagct atgataaatc
     3601 gtgctcgtga aatcattgag gaaattacac gtgaagcgga agtaggtcaa acttatcaag
     3661 ccactgttaa acgtattgaa aaatacggtg cgtttgtagg cctattccca ggtaaagatg
     3721 cgttgcttca catttcacaa atttcaaaaa atagaattga aaaagtggaa gatgtattaa
     3781 aaatcggtga cacaattgaa gttaagatta ctgaaattga taaacaaggt cgagtaaatg
     3841 cttcacatag agcattagaa gaataatatt taaagtcata tgacgacaat gtatcgtcat
     3901 gtgatttttt tatgccactt tttacgaagt gacccgtttt gaatttgttg tattgaacat
     3961 tttaaaacgc tttattattt tgtgtgcaac tgttaattat cctgtatgta tagtgattaa
     4021 tagtgtacat caagtgtttt ttaacttata atgaatagtg agtttatata tggacgggta
     4081 acaaatttag gaggtaagat tttgagttta ataaagaaaa agaataaaga tattcgcatt
     4141 ataccattag gcggtgttgg cgaaattgct aaaaatatgt atatcgttga agtagacgat
     4201 gaaatgttta tgttagatgc tggacttatg tttccagaag acgaaatgct aggtattgat
     4261 attgttatac cagacatttc atacgtactt gaaaataaag ataaattgaa gggtatattc
     4321 cttacacacg gacatgagca cgcgattggt gcagtgagtt atgttttaga acaattagat
     4381 gcaccagtat atggatctaa attgacaata gcgttaatta aagaaaatat gaaagcccgt
     4441 aatattgata aaaaagttcg ctactataca gttaataatg attcaattat gagattcaaa
     4501 aacgtgaata ttagtttctt taatacgaca cacagtattc ctgatagttt aggtgtttgt
     4561 attcacactt catatggtgc cattgtgtat acaggtgaat ttaagtttga ccaaagttta
     4621 catggacatt atgcaccaga tattaaacgt atggcagaga ttggtgaaga aggcgtattt
     4681 gtcttaatca gtgattctac tgaggcagag aaacctggat ataatactcc ggaaaatgtg
     4741 attgaacatc atatgtatga tgcttttgca aaagtgcgag gtcgcttgat agtttcatgt
     4801 tatgcttcga actttatacg tattcagcaa gttttaaata ttgctagcaa gctaaatcgt
     4861 aaagtgtcat ttttaggaag atcacttgaa agttcattta atattgctcg taaaatgggg
     4921 tatttcgaca ttcctaaaga tttgctaatt cctataacag aagttgataa ttatcctaaa
     4981 aatgaagtga taattatagc tactggtatg caaggagaac ctgtagaagc cttaagtcaa
     5041 atggcgcaac ataagcataa aattatgaat atcgaagaag gcgattctgt atttttagca
     5101 attacggctt ctgctaatat ggaagttatc attgcgaata cattaaatga gcttgtacgt
     5161 gctggcgcac atattattcc aaataacaaa aagattcatg cttcaagtca tggttgcatg
     5221 gaagaattaa aaatgatgat taatattatg aaacctgaat actttattcc tgtacaaggt
     5281 gaatttaaaa tgcagatagc acatgcgaag ctagcagctg aagcaggtgt tgcaccagaa
     5341 aagattttcc ttgtggaaaa aggagatgtc attaattaca acggtaaaga tatgatatta
     5401 aatgaaaagg taaattcagg aaatatttta atagatggca ttggtattgg ggatgtagga
     5461 aatatcgtgt tgagagaccg tcatctttta gcagaagatg gtatctttat tgctgttgta
     5521 acgttagatc ctaaaaatag acgtatagct gcgggacctg aaattcaatc tcgtgggttt
     5581 gtatatgtac gtgaaagtga agacttatta cgtgaagcag aagagaaagt acgtgaaata
     5641 gtagaggctg gtttacaaga aaaacgcata gaatggtctg aaattaaaca aaatatgcgt
     5701 gatcaaatta gtaaactatt attcgaaagt acaaaacgtc gtcctatgat tattccagta
     5761 atttctgaaa tttaatcaaa aagtcattaa cataaaagag gtcagaacaa gtcactgaaa
     5821 tataatggtt gtcatggaca atttacttat attttatgat ggtcaattga aggggtaacg
     5881 attaatctgt tatcttaagt aaattgatac atagatgata ttgttctaac ctctttcatc
     5941 gtctgtttgg actacatatt ctaaacatca aataggaaat tatatataat aacgtcgttt
     6001 taactaaggc aacataagga ggtgcgtcaa ttggcacaag caaaaaagaa atcgacagct
     6061 aagaaaaaaa caacatcaaa aaaaagaaca aattcgagga aaaagaagaa tgataatccg
     6121 atacgttatg tcatagctat tttagtagtt gtattaatgg tgttgggtgt tttccaatta
     6181 ggaataatag gtcgtctaat tgacagcttc tttaattatt tatttgggta cagtagatat
     6241 ttaacatata ttttagtact cttagcaact ggttttatta catactctaa acgtattcct
     6301 aaaactagac gaacggctgg ttcgattgta ttgcaaattg cattgctatt tgtatcacag
     6361 ttagtttttc attttaatag tggtatcaaa gctgaaagag aacctgtact ttcttatgtg
     6421 tatcagtcat accaacacag tcatttccca aattttggtg gcggtgtatt aggcttttat
     6481 ttattagagt taagcgtacc tttaatttca ttatttggtg tatgtattat tactatttta
     6541 ttattatgct caagtgttat tttattaaca aaccatcaac atcgtgaagt tgcaaaagtt
     6601 gcactggaaa atataaaagc ttggtttggt tcatttaatg aaaaaatgtc ggaaagaaac
     6661 caagaaaaac aattgaagcg tgaagaaaaa gcaagactta aagaagaaca aaaggcacgt
     6721 caaaatgaac agccacaaat aaaagatgtg agtgatttta cggaagtgcc tcaagaaaga
     6781 gatattccaa tttatgggca tactgaaaat gaaagtaaaa gccagagtca accaagtcga
     6841 aaaaaacgag tgtttgatgc agagaatagt tcgaataaca tcgtaaatca tcatcaagca
     6901 gatcagcaag aacaattaac agaacaaact cataacagtg ttgaaagtga aaacactatt
     6961 gaagaagctg gtgaagttac gaatgtatcg tatgttgttc caccgttaac tttacttaat
     7021 caacctgcaa aacaaaaagc aacatctaaa gctgaagtgc aacgtaaagg acaagtacta
     7081 gagaatacat taaaagattt tggggtaaat gcaaaagtga cacaaattaa aattggtcct
     7141 gcagtaactc aatatgaaat tcaaccagct caaggggtta aagtgagtaa aattgtaaac
     7201 ttgcataatg atattgcatt agctttagca gcaaaagatg ttagaatcga agcgccaata
     7261 cctggtcgtt ctgcagtagg tattgaagtg ccaaatgaga aaatttcatt agtttcacta
     7321 aaagaagttt tagatgaaaa attcccgtct aataataaac tagaagttgg attaggaaga
     7381 gatatatcag gtgatccaat tactgttcca ctaaatgaaa tgccacactt attggtggca
     7441 ggatcgacgg gtagtggtaa atctgtttgt ataaatggta ttattacaag tattttatta
     7501 aatgctaagc cgcatgaagt taaacttatg ttaatcgatc cgaaaatggt tgaactaaat
     7561 gtttataacg gaattccaca cttattaatt ccggttgtta caaatcctca taaagctgct
     7621 caagctttag aaaaaattgt agctgagatg gaaagacgtt atgatttatt ccaacattca
     7681 tcaactagaa acattaaagg ttataacgaa ttaatccgta agcaaaatca agaattagat
     7741 gagaagcaac cagaattacc ttatatcgtt gttattgtag atgagcttgc agatttaatg
     7801 atggtagctg gtaaagaagt tgaaaatgcg attcaacgta ttacacaaat ggcacgtgca
     7861 gcaggtatac atttaattgt agcgacacaa agaccttctg tggatgtaat tacaggtatc
     7921 attaaaaata atattccatc tagaatagct tttgctgtga gttctcaaac agattcaaga
     7981 actattattg gtactggcgg cgcagaaaag ttacttggta aaggtgacat gttatacgtt
     8041 ggaaatggtg actcatcaca aacacgtatt caaggggcgt ttttaagtga ccaagaggtg
     8101 caagatgttg taaattatgt agtagaacaa caacaggcaa attatgtaaa agaaatggaa
     8161 ccagatgcac cagtggataa atcggaaatg aaaagtgaag atgctttata tgatgaagcg
     8221 tatttgtttg ttgttgaaca acaaaaggca agtacatcat tgttacaacg ccaatttaga
     8281 attggttata atagagcatc taggttgatg gatgatttag aacgcaatca ggtaatcggt
     8341 ccacaaaaag gaagcaagcc tagacaagtt ttaatagatc ttaataatga cgaggtgtaa
     8401 aaaaatgtca gaaatgaatg cggtatataa cgttaaacaa tacattttaa atttgattaa
     8461 gcaaaataaa ttggaatatg gtgaccaact tccaagtaat ttatcaattg ccagagaatt
     8521 aaatgtaaaa accgacgatg tttatgaagc aattcaggca ttgattactg aacaagtcat
     8581 taaagataat tttgaagagg gcacaagtgt taagtcactg ccccctttct tttatccatt
     8641 gaatgaactt ataagtattg ggcaaatgat taaaaatgca ggatttgaat gcggaactga
     8701 atacttaaat tttgatgagc aaccagcaac tatgttagat gcaaatttgt tgagcgttga
     8761 agaaggatat ccagtaacca ttatagaacg attacgaact gccgatggag aaccggtcgt
     8821 ctattgttta gataaaattg ctaaaaaaga attaacatgt acagagtatc aaatgagcaa
     8881 tggatcgatt ctaagtgcga taaaagaaca aagtaatcat aatatttgtt atgctgatac
     8941 agaaattgaa gcggtaaatt atgaacctcg aatatccgaa gtactgaatg cttcgccaca
     9001 cgaaggtttg attttattaa aaattacgca ctataatgaa tcagatgaac ctattttgta
     9061 ttcattaaat tatatgaaaa atagcttagt tcaatttaaa atcactagaa aaatatagat
     9121 gtaagaagat atagaggagg ctatatattt gagtagacaa tctcaaccaa atatacatat
     9181 caaagtttca ccaacaacta aatttaaaac aactactata gtttttaaat ttatggcacc
     9241 tttagaatat gacacaataa cagctagatc attattaagc aaattattag ttcgagcaac
     9301 taagaaatgg ccaaccgata agtcgtttaa taatcactta gccgatttat atggtgcgta
     9361 tgtgaatagt acaatttcaa aattcaaaga tcagcatgtc attacatttt cattagaaat
     9421 tgttaatgaa cgttatttaa gaaacggtga atcattattt aatcaaggat tagatttatt
     9481 acaagaaatc atttggaatc cattaattga aaataaagca ttcaatgata attttgttaa
     9541 ccaagagaaa acattattag ccaaaaaaat agaagcaatg gtagataata aagcacaata
     9601 ttcgttttta aaattactcg accatatgtt tgaaaatgaa gcatataaat acttatctac
     9661 aggacaacta gaacaaatcc cacatattac tgctgaaaca ctatatcata catatcaatc
     9721 aatgattaat aatgatcaat gttctgttta tgttgtcggc aatgtagaac ctgaaagtgt
     9781 tgagaaacaa atacgtgaaa aatttgcact taaaccattc gataaacatc aattccaaca
     9841 ttctactcat catttacacg atgaagaagt tgattatatt gttgaatatg atgacgtgga
     9901 tcaagctaaa ttaaatatgg gataccgttt tccaacacaa tatggacaaa gtggatatgc
     9961 tgcctttgtt gtatttaaca tgatgtttgg aggagatcct tcatctgttt tatttaatga
    10021 agtgcgagaa aagcaaagtt tagcgtactc tatacattca caaattgatg gcaaaaatgg
    10081 ctatttattt gttttgagtg gggtttcaag tgataagtac gaaactgcaa aagacactat
    10141 tataagtgaa tttgaaaaaa taaaagcagg agatttcact gaagaaaaat tagagttagc
    10201 taaaaaagta atcatttctc atcgatatga atctgaagat cgtccgaaaa gtattataga
    10261 gattatgcat aaccaaatat tattagagca accacaaagc aaagaaacat ttataaatga
    10321 tatacagaag gtaagtcgcg aagatattgt ttctgttgct gaaaaagcat ttttagatac
    10381 aatctatgtg ttgacaaaag gaggggataa ataatgaaag agcgttatta tgaattaata
    10441 gacgaaagag tattcgaaca agaattagaa aatggtttac gattatttat tatccccaaa
    10501 ccaggttttc aaaagacatt tgtcacttac actacacaat ttggttcatt agataatcaa
    10561 ttcaaacccc ttggacaaga ccaatttgtt actgtacctg atggagttgc tcacttttta
    10621 gaacataaat tatttgaaaa agaagaagaa gacttattta ctgcgtttgc tgaagataac
    10681 gcacaagcaa atgcgtttac aagctttgat cgtacaagct acttgttcag tgcaactgat
    10741 aatattgaaa acaacattaa acgtttactt acaatggttg aaacgcctta ttttacaaaa
    10801 gaaactgttg ataaagaaaa aggtattatt gcagaagaaa taaaaatgta tcaagagcaa
    10861 cctggatata aattaatgtt tgatacattg cgtgcaatgt atcaacaaca tccaatacgt
    10921 gttgatattg ccggtagtgt agaaagtata tacgatatta caaaagatga tttgtatcta
    10981 tgttatgaaa cgttttatca tccatcaaat atggttttat ttgttgttgg cgatgtggat
    11041 cctgaagcaa tatgtcgaat agtaaaacaa cacgaggatg ctcgtaataa agttaaccaa
    11101 cccaaaatcg aacgaggact tgttgatgaa ccggaggatg ttaaagaagc atttgttact
    11161 gaatctatga aaattcaatc accaagacta atgcttggtt ttaaaaataa accattacaa
    11221 gaagcgcctc aaaaatatgt acaacgtgat ttagaaatgt cattattctt tgagttaatt
    11281 tttggggaag aaacagattt ttatcagaat ttattaaacg aaggacttat cgatgataca
    11341 tttggttatc aatttgtact agagccgacg tatagttttt caatcgtgac aagtgctact
    11401 gaagaaccag ataaattaaa aaaattatta ttagatgagt tgcgtgataa aaaaggcaat
    11461 ttccaagatg cagaagcatt tgaacttttg aaaaagcaat ttataggtga attcatatca
    11521 agtttaaact cacctgaata tattgctaat caatatacta aattgtattt tgaaggtgtt
    11581 agcgtgtttg acatgttaga cattgttgaa aatatcacgt tagatagtat taacgaaacg
    11641 tcatcgttat atttaaattt agatcagcaa gtcgatagtc gtttggagat taaaaagtaa
    11701 tgaaagcatt agtattaggt ggttctggtt caattggttc tgagatagtc aaacaattat
    11761 taactgatgg atttgaagtt tatgtgcaat attatcgtac tgatataaat gaattaacta
    11821 gcaaatttaa tgatgataaa gttcgtttta tacaagcgga tttatctcaa acaattgata
    11881 ttgacaaaac atttggtgac attaaatcat tagactgttt aatatatgca agtggtcagt
    11941 ctttatatgg tgttttacaa gatatgaaag accatgatat tgatgcatgt tatcagttaa
    12001 atgtcttgca attaattcga ttatgtagat atttcgttga tgttttacgt caaagtgcca
    12061 atggaagaat tattgtaatt tcatcaattt ggggtgagac aggagctagt atggaaacta
    12121 tttattcggc gatgaaaagt gcacaattag gtttcgttaa ggcgcttagt caagagcttg
    12181 cactaacatc agtgacagta aatgctatcg cacctggatt tgtagccggt aatatggcaa
    12241 gtgagtggca agaagatgaa cttcaagcaa tgataactga attaccacaa cagcgattgg
    12301 ttttaccgag tgaggttgct catacatgcg cctatttata tcacccaaat gctagaagtg
    12361 ttactggaac tatacagaaa gttaatggtg cttggtatat ttaaaatata gaactatttt
    12421 tatgagcaaa tgtttttatg attgatgtta taatatgaat attacaatga atcataattt
    12481 gttaattaaa caaggggtga ggttcaccat gacagttgca gagaaaaaag aatggtacct
    12541 agaatacgaa attgaaatta atagaccggg tcttttaggt gatgtatcta gtttattagg
    12601 tatgttaggt ataagtattg ttacaattaa tggtgttgat caaggtaaac gaggcctttt
    12661 aattaaaaca gacaatcttg aaaaagttga acgttttgag caaatagctc gtggtataaa
    12721 tgaaattgaa ataacaaagc ttaaaaaacc agaattaaga gaccgtcttg cagtaagaca
    12781 tggaagatat attgagcaag atgcaaaaga taagaaaacc tttcgatttg agcgtgaaga
    12841 tttaggcttg ttagtagact ttttagctga attgttcaaa gaagaaggtc ataagttgat
    12901 tggcattaga ggtatgccac gggttggtaa aactgaatca attgttgcgg gaagtgtttg
    12961 tgcacataag agatggttat ttattagttc tactttaata aaacaaactg tacgtagctc
    13021 tctaattaaa ggggaatatg atgccaatca tgtatacatt attgatggtg cagttactgc
    13081 cagagaatct aatccaaaac atcaagagct tgttaacgaa gttatgacgt taccatcaat
    13141 caaagtcgtt gaacatccag atttatttgt tgaaacaagt acttgtacaa tggaagattt
    13201 tgactatatt atcgaattga gagaaaatga aaatcaagaa atacattacg aagaaatgaa
    13261 gaaacagaca gtccaaagta agaataattt agattttgga gatccgtttg gtggtggttt
    13321 tggtttcttc gagtaagtgt aaggaggcta tgaattgaaa acggtcggtg aagcgctaaa
    13381 aggtagacgt gaaaggttag gaatgacttt aacagaatta gagcaacgta ctggaattaa
    13441 acgtgaaatg ctagtgcata ttgaaaataa tgaattcgat caactaccga ataaaaatta
    13501 cagcgaagga tttattagaa aatatgcaag cgtagtaaat attgaaccta accaattaat
    13561 tcaagctcat caagatgaaa ttccatcgaa ccaagccgaa tgggacgaag taattacagt
    13621 tttcaataat aataaagact tagattataa gagtaaatca aaagagccaa tacaattatt
    13681 agtaatc
//
LOCUS       DANJFZ010000067        13569 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.067, whole genome shotgun sequence.
ACCESSION   DANJFZ010000067 DANJFZ010000000
VERSION     DANJFZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.067"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            223..1704
                     /locus_tag="PWN45_001732"
     CDS             223..1704
                     /locus_tag="PWN45_001732"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL0623501.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            1688..1948
                     /locus_tag="PWN45_001733"
     CDS             1688..1948
                     /locus_tag="PWN45_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDL0623502.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1948..3510
                     /locus_tag="PWN45_001734"
     CDS             1948..3510
                     /locus_tag="PWN45_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL0623503.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3812..4615
                     /locus_tag="PWN45_001735"
     CDS             3812..4615
                     /locus_tag="PWN45_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL0623504.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4834..7158
                     /locus_tag="PWN45_001736"
     CDS             4834..7158
                     /locus_tag="PWN45_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL0623505.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7175..8533
                     /locus_tag="PWN45_001737"
     CDS             7175..8533
                     /locus_tag="PWN45_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL0623506.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8672..10183
                     /locus_tag="PWN45_001738"
     CDS             8672..10183
                     /locus_tag="PWN45_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL0623507.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(10363..10451)
                     /locus_tag="PWN45_001739"
     tRNA            complement(10363..10451)
                     /locus_tag="PWN45_001739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10414..10416),aa:Ser,seq:gct)
     gene            complement(10454..10528)
                     /locus_tag="PWN45_001740"
     tRNA            complement(10454..10528)
                     /locus_tag="PWN45_001740"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10494..10496),aa:Asn,seq:gtt)
     gene            complement(10670..11239)
                     /locus_tag="PWN45_001741"
     CDS             complement(10670..11239)
                     /locus_tag="PWN45_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDL0623508.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            11449..11667
                     /locus_tag="PWN45_001742"
     CDS             11449..11667
                     /locus_tag="PWN45_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDL0623509.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(11748..12734)
                     /locus_tag="PWN45_001743"
     CDS             complement(11748..12734)
                     /locus_tag="PWN45_001743"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL0623510.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            12933..13109
                     /locus_tag="PWN45_001744"
     CDS             12933..13109
                     /locus_tag="PWN45_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDL0623511.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            13124..>13569
                     /locus_tag="PWN45_001745"
     CDS             13124..>13569
                     /locus_tag="PWN45_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0623512.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICS"
BASE COUNT         4802 a         1868 c         2469 g         4430 t
ORIGIN      
        1 tatttttgga aacaattatg atattatacc atgttacaag atggttttaa taatttaaga
       61 tgagccataa ttgtaaaact aattcataat accgtatgtt ttatttttaa tagtagaaat
      121 tagaaaatgc tgattagtag gatataacag tgaaattata aatttattaa catcaacaaa
      181 acgtgtataa taaacatatt gtagaaaaag gagcggttca gtttggatgc aagtacgttg
      241 tttaagaaag taaaagtaaa gcgtgtattg ggttctttag aacaacaaat agatgatatc
      301 actactgatt cacgtacagc gagagaaggt agcatttttg tcgcttcagt tggatatact
      361 gtagacagtc ataagttctg tcaaaatgta gctgatcaag ggtgtaagtt ggtagtggtc
      421 aataaagaac aatcattacc agctaacgta acacaagtgg ttgtgccgga cacattaaga
      481 gtagctagta ttctagcaca cacattatat gattatccga gtcatcagtt agtgacattt
      541 ggtgtaacgg gtacaaatgg taaaacttct attgcgacga tgattcattt aattcaaaga
      601 aagttacaaa aaaatagtgc atatttagga actaatggtt tccaaattaa tgaaacaaag
      661 acaaaaggtg caaatacgac accagaaaca gtttctttaa ctaagaaaat taaagaagca
      721 gttgatgcag gcgctgaatc tatgacatta gaagtatcaa gccatggctt agtattagga
      781 cgactgcgag gcgttgaatt tgacgttgca atattttcaa atttaacaca agaccattta
      841 gattttcatg gcacaatgga agcatacgga cacgcgaagt ctttattgtt tagtcaatta
      901 ggtgaagatt tgtcgaaaga aaagtatgtc gtgttaaaca atgacgattc attttctgag
      961 tatttaagaa cagtgacgcc ttatgaagta tttagttatg gaattgatga ggaagcccaa
     1021 tttatggcta aaaatattca agaatcttta caaggtgtca gctttgattt tgtaacgcct
     1081 tttggaactt acccagtaaa atcgccttat gttggtaagt ttaatatttc taatattatg
     1141 gcggcaatga ttgcggtgtg gagtaaaggt acatctttag aaacgattat taaagctgtt
     1201 gaaaatttag aacctgttga agggcgatta gaagttttag atccttcgtt acctattgat
     1261 ttaattatcg attatgcaca tacagctgat ggtatgaaca aattaatcga tgcagtacag
     1321 ccttttgtaa agcaaaagtt gatattttta gttggtatgg caggcgaacg tgatttaact
     1381 aaaacgcctg aaatggggcg agttgcctgt cgtgcagatt atgtcatttt cacaccggat
     1441 aatccggcaa atgatgaccc gaaaatgtta acggcagaat tagccaaagg tgcaacacat
     1501 caaaactata ttgaatttga tgatcgtgca gaagggataa aacatgcaat tgacatagct
     1561 gagcctgggg atactgtcgt tttagcatca aaaggaagag aaccatatca aatcatgcca
     1621 gggcatatta aggtgccaca tcgagatgat ttaattggcc ttgaagcagc ttacaaaaag
     1681 ttcggtggtg gccctgttga ttaataaaag atttattgat gaaggtaaaa ctattgatgt
     1741 ttatttattc gaagcattaa ataaccagat aatcattgct ataccagatt ggttttggtc
     1801 atatcagatg gcaatgacat tagatgaaga aacttgtttt gaagcaatac tcatgcaatt
     1861 gtttgttttt aaagaagagg aagaggcaga atcgattgca tcacaactaa cagattggat
     1921 agaaacatat aaaaaggaga aagactaatg aacttaaagc aagaagttga gtctagaaag
     1981 acttttgcga ttatttcaca tcccgatgca gggaaaacaa cgttaactga aaaactattg
     2041 tacttcagtg gtgctattcg tgaagcgggt acagttaaag ggaagaagac tggtaaattt
     2101 gcgacaagtg actggatgaa agttgaacaa gagcgtggta tttctgtaac tagttcagta
     2161 atgcaatttg attacgatga ttataaaatc aatatcttag atacaccagg acatgaagac
     2221 ttttcagaag atacgtatag aacattaatg gcagttgaca gtgctgtcat ggtcatagac
     2281 tgtgcaaaag gtattgaacc acaaacattg aagttattta aagtttgtaa aatgcgtggt
     2341 attccaatct ttacattcat taataaatta gaccgagtag gtaaagaacc atttgaatta
     2401 ttagatgaaa tcgaagagac attaaatatt gaaacatacc ctatgaattg gccaattggt
     2461 atgggacaaa gtttctttgg catcattgat agaaagtcta aaacaattga accatttaga
     2521 gatgaagaaa atatattaca tttgaatgat gattttgagt tggaagaaga tcatgcaatt
     2581 acaaatgata gtgattttga acaagcgatt gaagaattaa tgttggttga agaagcgggt
     2641 gaagcctttg ataatgacgc gctgttgagt ggagacttaa cacctgtatt tttcggttca
     2701 gctttagcta actttggtgt acaaaatttc ttaaatgcat atgttgattt tgcgccaatg
     2761 ccaaatgcga gacaaacaaa agaagacgtt gaagtaagcc cgtttgatga ttcattttca
     2821 ggatttatct ttaaaattca agccaacatg gaccctaaac accgtgatag aattgccttt
     2881 atgcgtgtcg ttagtggtgc atttgaacgt ggtatggatg ttactttgca acgtactaat
     2941 aaaaagcaaa agatcacacg ttcaacgtca tttatggcag acgataaaga aactgtgaat
     3001 catgctgtag caggcgatat cattggacta tatgatactg gtaattatca aattggagat
     3061 actttagttg gtggaaaaca aacctacagt ttccaagatt taccacaatt tacgccagaa
     3121 atttttatga aagtttctgc taaaaacgtc atgaaacaga agcatttcca taaaggtatt
     3181 gaacaattag tacaagaagg tgcgattcaa tactataaaa cattacacac aaaccaaatt
     3241 attttaggtg ctgttggtca gttacaattt gaagttttcg aacatagaat gaaaaacgaa
     3301 tataatgttg atgttgttat ggagccagta ggccgtaaaa ttgcacgttg gattgaaaat
     3361 gaagaccaaa ttacagataa gatgaacaca tcaagatcga ttttagtgaa agatagatat
     3421 gacgatttag tattcttatt tgaaaatgaa tttgcaacaa gatggtttga agagaaattc
     3481 cctgaaatta aattgtatag tttactttaa cagctcaatt gtataatcga atttgttaca
     3541 ttaaaaataa ttgtttcgtt gaagaaaaat aaattgtata ttttaaaaga aaaaggtata
     3601 ctatgatgta tcaaatgaat aacctatggc attttgtcag aggggagtaa cttaagaatc
     3661 atgaccgtat aaatgattcg acactttatc gtcattacga agatatcttc cggtaaagtg
     3721 ggcaatttaa attgcttagt gagacctttg ctatttattt agcataggtc tttttgtttg
     3781 tacttaactt atttatttaa aggagttgta catgttaatg gatccaagtt tgatcttacc
     3841 ttatttatgg gtacttgtcg ttttagtatt tttagaaggc ttattagcag cagataacgc
     3901 gattgttatg gctgtaatgg ttaagcactt accacccgaa caacgtaaaa aagctttgtt
     3961 ttacggtttg ttaggtgcat ttgtatttag atttttagca ttattcttaa ttagtattat
     4021 cgcgaacttt tggtttattc aagctgcagg agcggtttac ttaatttata tgtcaatcaa
     4081 aaatctgtgg cagttcttta aacacccaga aattgaaagt cctgaagctg gagatgatca
     4141 tcattatgat gaatctggtg aagagattaa agcaagtaac aaatcattct ggggaactgt
     4201 gttgaaaata gaatttgcag atatcgcatt tgccattgat tctatgcttg ctgctttagc
     4261 tattgctgta acacttccta aagttggtat tcactttggt ggtatggact taggtcagtt
     4321 cgtagtcatg ttcctaggtg gaatgattgg tgttattcta atgcgttatg cagcaacatg
     4381 gtttgtagag ctattaaaca aatatccagg acttgaaggt gcagccttcg cgatcgttgg
     4441 ttgggtaggt gttaaattag ttgtcatggt attagcgcac ccagacatcg ctgtattgcc
     4501 tgagcacttc ccacatggcg tattatggca atctattttc tggacagtac taattggatt
     4561 agtaattatc ggttggttag gttcagttgt taaaaataaa aaatcgcata aataattgat
     4621 gtgaagcgga caatcttaat ttagtttaag gttgtccttt ttcatttaat tgagtgattt
     4681 atgaaaaatg gattttgaag aatgtgaatc aaaagatgcg atatagtatt aagaaaatgt
     4741 gccttttata tttagcattt tttcaataga aattatatag attttaaagc aaattaggtg
     4801 ttaatgtgtc ataatgataa gtgattttat tgaatggagt ggacattagt ggatattggt
     4861 aaaaaacatg taattcctaa aagtcagtac cgacgtaagc gtcgtgaatt cttccacaac
     4921 gaagacagag aagaaaattt aaatcaacat caagataaac aaaatataga taatacaaca
     4981 tcaaaaaaag cagataagca aatacataaa gattcaattg ataagcacga acgttttaaa
     5041 aatagtttat catcgcattt agaacagaga aaccgtgatg ttaatgagaa taaagctgaa
     5101 gaaagtaaaa gtaatcagga tagtaagtca gcatataaca gagatcatta tttaacagac
     5161 gatgtatcta aaaaacaaaa ttcattagat tcagtggacc aagatacagt gaaatcaaaa
     5221 tattatgagc aaaattctga agcgacttta tcaactaaat caaccgataa agtagaatca
     5281 actgaaatga gaaagctaag ttcagataaa aacaaagttg gtcatgaaga gcaacatgta
     5341 ctttctaaac cttcagaaca tgataaagag actagaattg attctgagtc ttcaagaact
     5401 gattcagaca gctcgatgca gacagagaaa ataaaaaaag acagttcaga tggaaataaa
     5461 agtagtaatc tgaaatctga agtaatatca gacaaatcaa atacagtacc aaaattgtcg
     5521 gaatctgatg atgaagtaaa taatcagaag ccattaactt taccggaaga acagaaattg
     5581 aaaagacagc aaagtcaaaa tgagcaaaca aaaacctata catatggtga tagcgaacaa
     5641 aatgacaagt ctaatcatga aaatgattta agtcatcata taccatcgat aagtgatgat
     5701 aaagataacg tcatgagaga aaatcatatt gttgacgata atcctgataa tgatatcaat
     5761 acaccatcat tatcaaaaac agatgacgat cgaaaacttg atgaaaaaat tcatgttgaa
     5821 gataaacata aacaaaatgc agactcgtct gaaacggtgg gatatcaaag tcagtcaact
     5881 gcatctcatc gtagcactga aaaaagaaat atttctatta atgaccatga taaattaaac
     5941 ggtcaaaaaa caaatacaaa gacatcggca aataataatc aaaaaaaggc tacatcaaaa
     6001 ttgaacaaag ggcgcgctac gaataataat tatagtgaca ttttgaaaaa gttttggatg
     6061 atgtattggc ctaaattagt tattctaatg ggtattatta ttctaattgt tattttgaat
     6121 gccattttta ataatgtgaa caaaaatgat cgcatgaatg ataataatga tgcagatgct
     6181 caaaaatata cgacaacgat gaaaaatgcc aataacacag ttaaatcggt cgttacagtt
     6241 gaaaatgaaa catcaaaaga ttcatcatta cctaaagata aagcatctca agacgaagtg
     6301 ggatcaggtg ttgtatataa aaaatctgga gatacgttat atattgttac gaatgcacac
     6361 gttgtcggtg ataaagaaaa tcaaaaaata actttctcga ataataaaag tgttgttggg
     6421 aaagtgcttg gtaaagataa atggtcagat ttagctgttg ttaaagcaac ttcttcagac
     6481 agttcagtga aagagatagc tattggagat tcaaataatt tagtgttagg agagccaata
     6541 ttagtcgtag gtaatccact tggtgtagac tttaaaggca ctgtgacaga aggtattatt
     6601 tcaggtctga acagaaatgt tcctattgat ttcgataaag ataataaata tgatatgttg
     6661 atgaaagctt tccaaattga tgcatcagta aatccaggta actcgggtgg tgctgtcgtc
     6721 aatagagaag gaaaattaat aggtgtagtt gcagctaaaa ttagtatgcc aaacgttgaa
     6781 aatatgtcat ttgcaatacc tgttaatgaa gtacaaaaga ttgtaaaaga tttagaaaca
     6841 aaaggtaaaa ttgactatcc cgatgtaggt gttaaaatga agaatattgc cagtctaaat
     6901 agttttgaaa gacaagcagt taaattgcca ggaaaagtta agaacggtgt tgttgtagat
     6961 caagttgaca acaatggttt agcagatcaa tctggtctga aaaaaggtga tgtaattact
     7021 gaattagatg gcaaactttt agaagatgat ttacgcttta ggcagattat atttagtcat
     7081 aaagatgact tgaaatcaat tacagcgaag atttatagag atggtaaaga gaaagaaatt
     7141 aatattaaac taaaataatg ttgaggtgaa caacgtgtca atttttagcc agtttttaaa
     7201 aagatcaagc cctcaacaag gtattgtatt gtactatatc gtcgcaattg tcattgcatt
     7261 tttattatta aacttaccgt atgttcataa accaggtgta gaagtaaatc caattgacac
     7321 attatttgtt gccgtatccg gaattagtgt tactggattg tctccgataa gtattgtcga
     7381 tacctattct acatttggac aattaattat cctcgtgata ttaaatattg gtggaattgg
     7441 cgtcatggca attggtacga tgttatgggt ggtactaggt aaacatattg gaattagaga
     7501 acgtcagtta attatgttag ataataacaa aaacacaatg agtggtaccg tcaaattgat
     7561 tattgatatt gtaaaatcaa tatttgtaat cgaactcgta ggagccatgt tattagcatt
     7621 ttacttttat cgagataatc cagatttaaa atatgcaatc atgcaaggtg tttttgtttc
     7681 tatttctgcc actaccaatg gtggattaga tattacaggt aagtcattaa ttccttatgc
     7741 acatgattat tttgtacaag cgatagttat atttttaata attttaggat caatcggctt
     7801 cccagtatta ttagaagtta aagcttatat tcaaaatagg gttactaatt ttagattttc
     7861 attatttact aaaattacga catcaacata tttattccta tttattgttg gggtattagc
     7921 cattctatta tttgaacata accatgcgtt caaaggttta agttggcatc aatcgttatt
     7981 ctattcgctg tttcaatcag cgactacaag aagtgcgggt cttcaaacaa ttgatgtgac
     8041 aacactaagt gaccccacta atattatcat gggtatttta atgtttatag gatcttcgcc
     8101 aagttcggtt ggtggcggta ttcgtacaac aactttcgct attttaattt tgtttttaat
     8161 taactttagt aataatgccg ataaaacatc cattaaagtt tacaatagag aagtacacat
     8221 tatggatatt caacgttcat ttgcagtatt tacaatggcg acaattttaa catttttagg
     8281 aatgctaatt atatcagcta ctgaaaatgg taagcttaca tttttacaag tattttttga
     8341 agtcatgtct gcatttggaa cttgtggact atcgcttggt gtcacaagtg atattagtga
     8401 tatttctaag gtcgtactaa tgatactcat gtttatagga cgtgttggct taatatcatt
     8461 tatcattatg atagcaggac gtcgagaacc agataaattc cattatccaa aagaacgtat
     8521 tcaaatagga taatataata gcaatctaag tttagttaat gtagatttta actggaactt
     8581 agattgcttt tttagtttgt atttttaact tattttataa gacgattggt ttcgaaaatg
     8641 gtaaactagt aacaatgaga ggtgtaacat gatggaaaaa aatgaaaaca ttaatgtaga
     8701 gattttaact acgtcagata tgcatagtca tttcttaaat ggtgattatg gttcaaatat
     8761 ttatagagct ggtacttatg ttaaccaagt aagagcacaa aatcatcgcg tcattttatt
     8821 agatagtggc ggaagtttag ctggctcgtt agcggcctat tattatgcta ttgttgcacc
     8881 ttataaacga catccaatga taaagttaat gaacagaatg cattatgatg ctagcggtgt
     8941 gagtccaagt gaattcaagt ttggtttatc atttttaact cgttcaattg ctttggcacg
     9001 ttttccatgg ttatcagcaa atattgaata caatgttact aaggagcctt atttttcaac
     9061 tccatattgt attaaacatt ttggtgactt aaaaattgct atcgtaggcg tcacagcaga
     9121 tggtttaatg gaaaatgagt attctgaaat ggagcaagat gtatctattg aaaagacatt
     9181 agtggcatca aaacgttgga ttagatatat ccatgaagtt gaagagccag attttttgat
     9241 tgtaatttat catggtggat tgaataaaat tagtaatagt acgaaaaata aaaaggcaag
     9301 ttcgaatgaa gctgaaaaat taatggaaga actcggtgtt atagatttaa tgattacagc
     9361 tcatcagcat caaacaatag taggtcaaga tcatgaaacg tattatgttc aggctggtca
     9421 agatgccaaa gagcttgtac atctttcgat taattttaaa aagagaacaa caacttatga
     9481 tgttgaaagc attgattcta aagtgattga cttaaatgag tatgaagagg atcaagaatt
     9541 attagattta acattctatg atagaaaagc agtggcttat tggtcacagg aaatcataag
     9601 tgataaaggt ttgatgttat cagtaaatgg gttacaagat ttagtctgtc aaacacatcc
     9661 attttcgcaa ttattacatg atgcaattca ccttgcattt gataatgata taacatgtgt
     9721 ccacgtgcct atgaacggag agaaggggtt gagtggacag attcgaaatg aagatttgta
     9781 tcatgcatac ccatatccag ataagccaat ggatatgaca attagtggtc aaaatatcaa
     9841 agatatattg gagtatagtt attcacattt agattttgtt aacgagcaat taagcttaac
     9901 aattattgat gaaacgttat gtacaatgtg gcaaggattc aattatgaga ttgatatgaa
     9961 tcaagaacct gggcaacgag taatgttaga tcaaattgat ttgactaaga gttatagagt
    10021 tacaatgact gactattgtt atcgtaacta caagaattat ttaaaaaatg ctattataca
    10081 tgaatcatac gatgaaacaa tgagtacatt aattgcagag aagttaagag atccgaatta
    10141 tcgtatttca tgtagtgata attttatagt taaaaacagg taaatattgc tatgtaataa
    10201 attaattata agttcaacaa gtataaaaac ctttcttaga gaagattatc taggaaaggt
    10261 ttttagaatt ttaaaattga tataaaagaa cagtgaaatt ctcattcatt atatataagt
    10321 aaacaaaaaa gctaaaatgg tatataccat tttagcaagt aacggaaacg gagggattcg
    10381 aaccctcgcg ccgctttcgc gacctacacc cttagcaggg gcgcctcttc agccaacttg
    10441 agtacgtttc caatggctcc acaggtagga ctcgaaccta cgaccgatcg gttaacagcc
    10501 gatagctcta ccactgagct actgtggatt aatttgtatg tgtttgaata tcaagcacaa
    10561 atattattat aacaagatta agttaatttg caatagatac cttaaaaaaa tatgcagaac
    10621 atttacacta tatttataaa acattccgca tattttaaaa ataaacaatt tatttatctt
    10681 caaataaaga atagcgtgtc aatgcttcaa acacattcaa tgtggcttta ttataatcta
    10741 ttggttggtc gggatttttt gatatcatgc gagattgttt atctaccatg taatagtaaa
    10801 aaatggaatt gttatattga tgccataaac tgtggtatga aacatgaaga atgattgatt
    10861 cattattaat aaatgtcact ttacatttac gattttttaa ttctttaata ctttcgatat
    10921 aatgcatatt taaccaaata ttttcatttt gtctgtcaga atgtgttggg aaaaaataag
    10981 ttggaaataa tggtgttagt aaaataggtg gtttactaga aatgccagta atgcggtttg
    11041 tctctgcttt cttgccaaga taagtattac cataaaattt gcaagagcgt tcaatgattt
    11101 tctggacttt aaaaggattt tgaatacgcg ttttgtcaaa ccgaattatt tcactaccgt
    11161 ttctttgatc gtcatcatca aatgctggtc gaataaccat gtctcctttg cgtatcacat
    11221 aaatattttg agaatacata agcaaaacct cgctttatta agttttaaac cattaatata
    11281 aaaatacaat taatgatata aagatatttt ataataaaat tctgagttgt caagttatta
    11341 ttttggtgaa aatgtacaat aatttttaac taatagtcag aatattcatt gatttcaaaa
    11401 ttagaaaaat gtatacttat tataaattaa agaaaggtag tggtttctat gaaatacaat
    11461 actaatgtta aacatacaac tttagaagcg tttgtcacaa ctgtcaatga tttgggtatt
    11521 gaattaatta tcaatgaagc acttcgagag gtaagaaaac gacagctcat agaacttata
    11581 gatgacgcac tcgtcaataa agatgaagca gcatttaatc aatatacggc agaatacaaa
    11641 aatttggagg catttctcgg tgaataacat tgattcgaat tccataacgc aatcataaaa
    11701 ggactattct taacatctca tacatgtaag aatagtcctt taatcgttta tgacattaat
    11761 ctaattaatt catgtctatc tatgtcaccg aaataatgat ataaatcata ttctgatagt
    11821 gcttcttcta tgtgctcaaa gtcgtgtaga cagccaacta atgcattttc aagatcagtg
    11881 acatctccga caccaaagaa atcaccgaat atttttgcat gttcgatttt acctcgttta
    11941 acatcaaact taatttgtac aaatcctttt tcaaattttt cctcgcgttc aaagttatat
    12001 ttagggtttc taccataatt ccattcccaa gttctatatt tgtcgttact taacttttca
    12061 atattttccc aatcttcatc cgttaattta tattcttcta cttcagtttc tccaaagata
    12121 gttttcaaga tgattttttt aaattcttca atttctaaag ggtcatttaa aaattcttga
    12181 atgtttgcta cccgtttacg aacagattta ataccttttg atttaatctt agctggatta
    12241 acttttagtg cattctgaac ttcgtctaaa tcactattta acatcaacgt accatgacta
    12301 aacattctat ttttaacttt aaccatagca ttccctgaga ttttcgcttg tccaacttga
    12361 atatcgttac gaccagttaa ttcagcattt acgcctaaag attgtaatgc ttgaacaatt
    12421 ggttcagtga atttttggaa attgtggaaa ctgttaccat catcatctgt tataaaacta
    12481 aagtttaaat tgccagtatc atgataaaca gcgccaccac cagaaattct tcttactaca
    12541 tcgatgttgt gagcatcgat atatgtctga tttacttcct ctatcgtatt ttgattcttt
    12601 ccaacaatga tagatggtct atttatgtaa aataaaaagt aactttcttc tgctggtaaa
    12661 ttttttaaaa catattcttc cattgctaag tttaaagttg gatctgtaat attattatta
    12721 ctaatgaatt tcattacaat ctctccctta tatctatata tattctctac ttatttatgc
    12781 cttaactttg ctcaaaatgc caattatata ctcacaaatc attctaaata tcggcttaaa
    12841 gttacatttt attatttttg attgttaata gtttatttgt taagtgaatt ttagtacaat
    12901 ataatttagt tatgatttaa ggaggactta aaatgactgt tgcagaagtg ggtaacattg
    12961 ttgagtttat ggatggatta agaggtcgtg ttgaaaaaat caacgataac tctgttattg
    13021 ttgacttaac aattatggaa aattttaatg accttgattt accggaaaaa actgttatca
    13081 atcataaacg atataagatt gttgaataag aaggtaagtt ataatgaata aaatctcgaa
    13141 ggctttaact tggtttatta taagtttcat tatatttcat ctcatattat ttattatgtg
    13201 gggcgaacac caagaatact ggtatttata tacaggtata atgctaattg ctggtatcag
    13261 ttatgtattt tatcaaagag atattgaatc taagcggttg cttacatcaa ttggtgttgg
    13321 tattattacg gcaattattt taattatgct tcaactttta ttctcactta taacttctaa
    13381 tttaagttat agttcattaa ttaaagaatt agcaagaaca ggtgtcaatt ggaagtggca
    13441 aatgttagta actttacttt ttgtcattcc atgtcacgag ttatatatga gaactgtttt
    13501 acaaaaagaa ttaacacatt tttcaatacc gaaatgggta gctatattaa taactgcaat
    13561 ttgttctag
//
LOCUS       DANJFZ010000068        13427 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.068, whole genome shotgun sequence.
ACCESSION   DANJFZ010000068 DANJFZ010000000
VERSION     DANJFZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.068"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..77
                     /locus_tag="PWN45_001746"
     CDS             <1..77
                     /locus_tag="PWN45_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HDL0623513.1"
                     /translation="FDHNPGIIPDYEINSWKQLNDIVR"
     gene            complement(186..626)
                     /locus_tag="PWN45_001747"
     CDS             complement(186..626)
                     /locus_tag="PWN45_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0623514.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            821..2434
                     /gene="bshC"
                     /locus_tag="PWN45_001748"
     CDS             821..2434
                     /gene="bshC"
                     /locus_tag="PWN45_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDL0623515.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            2578..3009
                     /gene="mraZ"
                     /locus_tag="PWN45_001749"
     CDS             2578..3009
                     /gene="mraZ"
                     /locus_tag="PWN45_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL0623516.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            3025..3960
                     /gene="rsmH"
                     /locus_tag="PWN45_001750"
     CDS             3025..3960
                     /gene="rsmH"
                     /locus_tag="PWN45_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL0623517.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            3974..4375
                     /gene="ftsL"
                     /locus_tag="PWN45_001751"
     CDS             3974..4375
                     /gene="ftsL"
                     /locus_tag="PWN45_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL0623518.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            4356..6590
                     /locus_tag="PWN45_001752"
     CDS             4356..6590
                     /locus_tag="PWN45_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0623519.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            6882..7847
                     /locus_tag="PWN45_001753"
     CDS             6882..7847
                     /locus_tag="PWN45_001753"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL0623520.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            7849..9198
                     /locus_tag="PWN45_001754"
     CDS             7849..9198
                     /locus_tag="PWN45_001754"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL0623521.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            9214..10533
                     /locus_tag="PWN45_001755"
     CDS             9214..10533
                     /locus_tag="PWN45_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL0623522.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            10639..12051
                     /gene="ftsA"
                     /locus_tag="PWN45_001756"
     CDS             10639..12051
                     /gene="ftsA"
                     /locus_tag="PWN45_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL0623523.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            12084..13256
                     /gene="ftsZ"
                     /locus_tag="PWN45_001757"
     CDS             12084..13256
                     /gene="ftsZ"
                     /locus_tag="PWN45_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL0623524.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         4973 a         1841 c         2572 g         4041 t
ORIGIN      
        1 gatttgatca taatccagga attatacctg attatgaaat taattcatgg aaacaactaa
       61 atgatattgt acgttaagac aaataatttg aaaatgattt acttatatta gcttaagaaa
      121 gtaattgtaa aaaagacact acgaaaagat gcattttcat gctcgtagtg tcgaatatta
      181 ttatcttatg gtttccacaa taagacatca tgtccctcta tatttttagc ttccacatct
      241 gtaaatccat gatgtaaaaa gaaatcctta gaatcatttc tgccaatggc tttaattggc
      301 atattgaaac tctttgcaaa atcaatcaat tctgaagcgt accctctgtt ttgatatttt
      361 ggtaatactt ctaacttcca taataatata taatcttcaa aatctgggaa gtagatttct
      421 tcgacatcac ctttttttag taatgccatt ctagctccta attgatctcc gacaaatatg
      481 ccataaaatg gtgaatctga acttgcatca atcatttgac cgtttaactc attgaccatg
      541 tataagtctt tgttgccaaa cgctctaaag ttttcgaata attcgtcagt tttgtaatta
      601 atttcaagac gtttgatttc actcatattt atctacccct tatttgttta ctactattat
      661 tatacattaa accacttgtg tttttcattg atttgaaaat gtaaaacaat gaatattata
      721 aattttccat gattaaattg attttcaagc cgtaaaattg tcaatcaccg gtatagactt
      781 tatactatag tttgtaatcg cgtacttaag gagaaaaaac atggactgta aagtagttag
      841 tttaaatgaa aaagatcagt ttataccaaa aataaagagc agtgaccctg taataacagg
      901 attatttcaa tatgatgcag ctcaacaaac tagttttgaa aaaaggatgt ctaaagaaaa
      961 taatggaaga gaagcggcat tagcgaatgt tattcgtgaa tatatgagtg atttaaagct
     1021 ttcaagtgaa caagaattaa acatacaaca tttagctaat ggttcaaaag ttgtgattgg
     1081 tggacaacaa gcagggcttt tcgggggacc attgtataca ttccataaaa tattttcaat
     1141 cattacttta tctaaggaat taacggatac acataagcaa caagtagtac cagttttttg
     1201 gattgcagga gaagatcatg atttcgatga agtgaatcat acatttgttt ataacgaaaa
     1261 tcatgggtcg ctgcataagg ttaaatatca tacaatggag atgccagaga cgactgtctc
     1321 tagatattat cctgataagg ctgagttgaa acaaacttta aaaacgatgt tcattcatat
     1381 gaaagaaact gttcatacac aaggtctact ggagatttgt gacagaatta ttgaccaata
     1441 tgactcgtgg actgatatgt ttaaagcact actgcatgaa acatttaaag catatggcgt
     1501 tctatttata gatgcgcagt ttgagccgtt aagaaaaatg gaagcgccta tgtttaaaaa
     1561 gattttgaaa aaacatcagt tgcttgatga tgcttttaga gcaacacaac aacgtactca
     1621 aaatcaaggc ttgaatgcga tgatacaaac agatacaaat gttcatttat tcttacatga
     1681 tgaaaatatg cgtcaattag tttcgtatga tggtaagcat tttaaattaa ataaaacaga
     1741 taagacatat ataaaggaag aaattataaa tattgcggaa aatcaacctg aattattttc
     1801 taataatgta gtgacaagac cattaatgga agaatggtta tttaacacgg tggcatttgt
     1861 tggaggaccg agtgaaatta agtactgggc tgaactaaaa gatgtatttg aactatttga
     1921 tgttgaaatg cctatcgtga tgccaaggct tagaattact tatttaaatg accgtataga
     1981 aaaattactt tcgaaataca atattccatt agaaaaagtg ttagtcgatg gtgttgaagg
     2041 agaaagaagt aagtttatta gagaacaagc atcacatcaa tttattgaaa aggtagaagg
     2101 tatgattgaa caacagcgtc gtctaaacaa agacttatta gatgaagtgg cggggaatca
     2161 aaataatatt aaccttgtga ataaaaataa tgaaattcat atacaacagt atgattattt
     2221 gttaaaacgt tatcttttaa acattgaaag agaaaacgac atcagtatga agcaatttag
     2281 agaaattcaa gaaacactcc atccaatggg aggattacaa gaaagaatat ggaatccact
     2341 tcaaattttg aatgattttg ggacagatgt gttcaagccc tccacctatc caccactttc
     2401 ttacactttt gatcatatta ttataaaacc ttaatatacc aagggtttag cccgatttat
     2461 cttaatgata aatcgggcat ttttttgttt tttaaaataa atttcacaaa ttttgtataa
     2521 atagtggtgg atagtgggga gatgtggtaa attatatata aggtgaggtg ataaaaaatg
     2581 ttcatgggag aatacgatca tcaattagat acaaaaggac gtatgattat accgtccaag
     2641 tttcgttatg acttaaatga gcgttttatt atcacaagag gccttgataa atgtttattc
     2701 ggttacactc tagacgaatg gcaacagatt gaagagaaaa tgaaaacctt acctatgaca
     2761 aaaaaagacg cacgtaagtt tatgcgtatg ttcttctctg gtgctgttga agtagaactt
     2821 gataagcaag ggcgtattaa catccctcaa aacttgagga aatacgctaa tttaactaaa
     2881 gaatgtacag taatcggtgt ttcaaatcgt attgagattt gggatagaga aacttggaat
     2941 gatttctatg aagaatctga agaaagtttc gaagatattg ctgaagattt aatagatttt
     3001 gatttttaaa atggaggaat tgaagtgttt catcatatca gcgttatgtt aaacgaaacc
     3061 attgattatt taaatgtaaa agaaaatggt gtgtacattg actgtacgct aggtggagcg
     3121 ggacatgccc tttatttact aaatcaatta aatgacgacg gaagattaat agcaatcgat
     3181 caagaccaaa ctgcaattga taatgctaaa gaggtattaa aggatcattt gcataaggtg
     3241 acttttgttc atagcaactt ccgtgaatta actcaaatat taaaagactt aaacattgaa
     3301 aaagtagatg gaatttatta cgacttgggt gtttcaagcc cacaactcga cattccagaa
     3361 cgaggattca gttatcacca tgacgcaaca ttagacatgc gtatggacca aacacaagaa
     3421 ctaacagcat atgaaattgt taacaattgg tcatatgaag cgttagtgaa gattttttat
     3481 cgctatggcg aggagaaatt ttcaaaacag atagctcgaa gaatcgaagc acatcgcgaa
     3541 caacaaccaa taacaacaac attagaatta gttgacatta taaaagaagg tattcctgca
     3601 aaagcaagaa gaaaaggcgg acatcctgca aaacgagtat ttcaagcact acgaattgca
     3661 gtaaacgatg aattgtcagc ttttgaagat tcaatagaac aagcgattga attagtgaaa
     3721 gtagatggca ggatttcggt aatcactttc cattctttag aagatcgttt atgtaaacag
     3781 gtgttccaag aatatgaaaa aggtccagag gtaccaagag gattaccagt tataccagaa
     3841 gcatatacac ctaagttaaa gcgtgttaat cgtaaaccga ttaccgctac agaagaagat
     3901 ttagatgaca ataacagagc acgaagcgcg aaattacgtg tagctgaaat acttaaataa
     3961 ggagcaattt ataatggctg tagaaaaagt gtaccaacca tatgacgaac aagtttataa
     4021 tagtataccg aagcaacaac cacaaactaa gcccgaaaag aagactgttt cgagaaaagt
     4081 ggttgtacaa ttaactaaat ttgaaaaagt tttatacata actttgatta ctgtaattgc
     4141 tatgttaagt atttatatgc tatctttaaa aatggatgcg tatgatacgc gaggaaagat
     4201 tgcagattta gattataaaa tagataaaca atcaagtgaa aacagtgctt tacaatctga
     4261 aatcaaaaag aattcttctt atgaacgcat atacgaaaag gctaagaaac aggggatgag
     4321 ccttgagaac gataatgtaa aggtagtgcg tagtaatggc gaagcaaaaa attaaaatta
     4381 aaaaaaataa aataggggca gtcctacttg ttggtttatt cggactgctc ttttttatat
     4441 tggttttaag aatttcatat atcatgatta ctggacattc taatggtcaa gatttagtca
     4501 tgaaggcaaa tgaaaagtat ttagttaaga atgcacaaca accagaacga ggaaagatat
     4561 atgatcgtaa tggtaaagtg ctagcagaag atgtagaaag atataaactt gttgcagtaa
     4621 tagataaaaa ggcgagtgcc aattctaaaa aacctaggca tgtagttgat aaaaaagaga
     4681 ctgcaaagaa attatctaca gtcattaata tgaagccaga ggaaattgaa aagagactta
     4741 gtcaaaagaa agctttccaa attgaatttg gacgcaaagg aacaaattta acgtatcagg
     4801 acaaattgaa aatagagaaa atgaatttgc ctggtatttc tttattgcct gaaacagaac
     4861 gcttttatcc aaatggcaat tttgcatcac acttaattgg tagagctcag aaaaatccgg
     4921 atactggtga acttaaaggt gcacttggag ttgaaaagat ttttgatagt tatttaagtg
     4981 gatctaaagg atcattgaga tatattcatg atatttgggg atatatcgca ccaaatacta
     5041 aaaaagagaa gcagcctaaa cgtggtgatg atgtccattt aacaatcgat tcaaatattc
     5101 aagtatttgt tgaagaagct ttagatggca tggttgaaag ataccagccg aaagatttat
     5161 ttgcggttgt catggatgcc aaaactggag aaattttagc atacagtcag cgaccaacat
     5221 ttaatcctga aactggtaaa gactttggta aaaagtgggc aaatgacctt tatcaaaaca
     5281 catacgagcc tggatcaaca tttaaatcat atgggttagc agctgctatt caagaaggtg
     5341 cttttgatcc tgataagaaa tataaatctg gacatagaga tattatgggt tcacgtattt
     5401 cagactggaa tagagtcggt tggggtgaaa tcccaatgtc actcggattt acttattcat
     5461 ctaatacatt gatgatgcat ttacaagatt tagttggtgc agacaaaatg aaatcttggt
     5521 atgaacgatt tggatttgga aaatcaacta aaggtatgtt tgatggagaa gcacctggtc
     5581 aaattggatg gagtaatgag ttgcaacaaa aaacgtcatc atttggtcaa tcgacaacag
     5641 taacacctgt tcaaatgtta caagcgcaat cagcgttctt taatgatggt aatatgttaa
     5701 aaccatggtt tgtgaatagc gttgaaaatc ctgttagtaa aagacaattt tataaagggc
     5761 aaaaacaaat cgcaggcaaa ccaataacaa aagatactgc tgaaaaagtt gaaaagcaat
     5821 tggatttagt tgtgaatagt aagaagagtc acgctgcaaa ctatcgtatt gatggttatg
     5881 aggtcgaagg taagactggt acagcacaag tcgctgcacc taatggtggt ggatacgtta
     5941 aaggtccaaa cccatatttt gtaagtttta tgggtgacgc gccgaagaaa aatcctaaag
     6001 ttattgtata cgctggtatg agcttggcac aaaaaaatga ccaagaagct tatgaattag
     6061 gtgttagtaa agcgtttaaa ccaataatgg aaaatacttt gaaatattta aatgtaggta
     6121 aatcaaaaga tgacacatct aatgcagagt atagtaaagt gccagatgtt gaaggtcaag
     6181 acaaacaaaa agctattgat aatgtgagtg caaaatcatt agaaccagtt actattggtt
     6241 ctggcacaca aataaaagca caatctataa aagcagggaa taaagtctta cctcatagta
     6301 aagtactgtt attaacagat ggagacttaa ctatgcctga catgtcagga tggacgaaag
     6361 aagatgtcat tgcttttgaa aacctaacaa atattaaagt aaatttaaaa ggtagcggtt
     6421 ttgtgtccca ccaatcaatt agtaagggac aaaaacttac tgaaaaagat aaaatagacg
     6481 tagaattttc atcagagaat gtagacagca attcgacgaa taattctgat tcaaattcag
     6541 atgataagaa gaaatctgac agtaaaactg acaaggataa gtcggactaa caagaatggc
     6601 tacattaaat taggatgtat tgtcactatt tgtttttact aaagtaatgc tgtgatagaa
     6661 aacaacatct tcatttaatc tttataaata agtgttgata gagaatttta aggtagacga
     6721 ggtcagatta acttgtttgt tataggcaaa ctttttatac tggcctctta tgccgtaaaa
     6781 aagaaaaatt ataaattttg attacaactt tgtttattta attgaaaaat tgataatata
     6841 ttagtggtta aaaaagtctg gattaagaag aggagatagt tatgattttt gtatatgcgt
     6901 tattagcgct agtgattaca tttgttttgg tacctgtttt aatacctaca ttaaaaagga
     6961 tgaaatttgg tcaaagtatt cgagaagaag gtccacaaag ccatatgaag aagactggta
     7021 caccaacgat gggtggacta acatttctat taagtattgt gataacgtct ttggtggcta
     7081 ttatatttgt agatcaagct aatccaatca tactgttatt atttgtgacg attggttttg
     7141 ggttaattgg ttttatagat gattatatta ttgttgttaa aaagaataac caaggtttaa
     7201 caagtaaaca gaagtttttg gcgcaaattg gtattgcgat tatattcttt gttttaagta
     7261 atgtgtttca tttggtgaat ttttctacga gcatacatat tccatttacg aatgtagcaa
     7321 tcccactatc atttgcatat gttattttca ttgttttttg gcaagtaggt ttttctaatg
     7381 cagtaaattt aacagatggt ttagatggat tagcaactgg actgtcaatt atcggattta
     7441 caatgtatgc catcatgagc tttgtgttag gagaaacggc aattggtatt ttctgtatca
     7501 ttatgttgtt tgcactttta ggatttttac catataacat taaccctgct aaagtgttta
     7561 tgggagatac aggtagctta gctttaggtg gtatatttgc taccatttca atcatgctta
     7621 atcaggaatt atcattaatt tttataggtt tagtattcgt aattgaaaca ttatctgtta
     7681 tgttacaagt cgctagcttt aaattgactg gaaagcgtat atttaaaatg agtccgattc
     7741 atcatcattt tgaattgata ggatggagcg aatggaaagt agttacagta ttttgggctg
     7801 ttggtctgat ttcaggttta atcggtttat ggattggagt gcattaagat gcttaattat
     7861 acagggttag aaaataaaaa tgtattagtt gtcggtttgg caaaaagtgg ttatgaagca
     7921 gctaaattat taagtaaatt aggtgcgaat gtaactgtca atgatggaaa agacttatca
     7981 caagatgctc atgcaaaaga tttagaatct atgggcattt ctgttgtaag tggaagtcat
     8041 ccattaacgt tgcttgataa taatccaata attgttaaaa atcctggaat accttataca
     8101 gtatctatta ttgatgaagc agtgaaacga ggtttgaaaa ttttaacaga agttgagtta
     8161 agttatctaa tctctgaagc accaatcata gctgtaacgg gtacaaatgg taaaacgaca
     8221 gttacttctc taattggaga tatgtttaaa aaaagtcgct taactggaag attatccggc
     8281 aatattggtt atgttgcatc taaagtagca caagaagtaa agcctacaga ttatttagtt
     8341 acagagttgt cgtcattcca gttacttgga atcgaaaagt ataaaccaca cattgctata
     8401 attactaaca tttattcggc gcatctagat taccatgaaa atttagaaaa ctatcaaaat
     8461 gctaaaaagc aaatatataa aaatcaaacg gaagaggatt atttgatttg taattatcat
     8521 caaagacaag tgatagagtc ggaagaatta aaagctaaga cattgtattt ctcaactcaa
     8581 caagaagttg atggtattta tattaaagat ggttttatcg tttataaagg tgttcgtatt
     8641 attaacactg aagatctagt attgcctggt gaacataatt tagaaaatat attagcagct
     8701 gtgcttgctt gtattttagc tggtgtacct attaaagcaa ttattgatag tttaactaca
     8761 ttttcaggaa tagagcatag attgcaatat gttggtacta atagaactaa taaatattat
     8821 aatgattcca aagcaacaaa cacgctagca acacagtttg ccttaaattc atttaatcaa
     8881 ccaatcattt ggttatgtgg tggtttggat cgagggaatg aatttgacga actcattcct
     8941 tatatggaaa atgttcgcgc gatggttgta ttcggacaaa cgaaagctaa gtttgctaaa
     9001 ctaggtaata gtcaagggaa atcggtcatt gaagcgaaca atgtcgaaga cgctgttgat
     9061 aaagtacaag atattataga accaaatgat gttgtattat tgtcacctgc ttgtgcgagt
     9121 tgggatcaat atagtacttt tgaagagcgt ggagagaaat ttattgaaag attccgtgcc
     9181 catttaccat cttattaaag ggtgtgagta ttgatggatg ataaaacgaa gaacgatcaa
     9241 caagaatcaa atgaagataa agatgaatta gaattattta cgaggaatac atctaagaaa
     9301 agacggcaaa gaaaaagatc aaaggctaca catttttcta atcaaaataa agatgataca
     9361 tctcaacaag ctgattttga tgaagaaatt tacttgataa ataaagactt caaaaaagaa
     9421 gaaagcaatg ataaaaataa tgattctgct tctagtcatg cgaatgataa taatatcgat
     9481 gattctacag actctaatat tgaaaatgag gattatagat ataatcaaga aattgacgac
     9541 caaaatgaat cgaatgtaat ttcagtcgac aacgaacaac ctcaatcagc tcctaaagaa
     9601 caaaatagcg actcgattga tgaggaaaca gtaacgaaaa aagaacgaaa aagtaaagta
     9661 acacaattaa agccattaac acttgaagaa aagcggaagt taagacgtaa gcgacaaaag
     9721 cgaatccaat acagtgttat tacaatattg gtattgttga ttgctgttat attaatttac
     9781 atgttttcac cacttagtaa aattgcgcat gtaaatataa atggaaataa tcacgttagt
     9841 acttcaaaga taaacaaagt tttaggtgtt aaaaatgatt caaggatgta tacgtttagt
     9901 aaaaaaaatg ctattaatga tctcgaagag aatccattaa tcaaaagtgt tgagatacac
     9961 aagcaattac caaacacatt aaacgtagat atcacagaaa atgaaattat tgctttagtg
    10021 aaatataaag gtaaatattt acctttatta gaaaatggta aattgcttaa aggttcaaat
    10081 gatgtcaaaa ttaatgatgc acctgtcatg gatggtttca aaggtacaaa agaagatgat
    10141 atgattaagg cgttatctga aatgacacct gaagttagac gatatattgc cgaagtgaca
    10201 tacgccccaa gtaaaaacaa acaaagcaga attgaattgt ttacgacaga tggacttcaa
    10261 gtaatcggtg atatttcgac gatatctaag aaaatgaaat attatccgca gatgtcacaa
    10321 tcattatcaa gggatagttc gggtaaacta aaaacacgag gctatattga tttatcagtc
    10381 ggtgcttcat ttatcccata ccgtggaaac acgtctagtc aatcagaaag cgataaaaat
    10441 gtgactaaat catctcaaga ggaaaatcaa gcaaaagaag aattacaaag cgttttaaac
    10501 aaaattaaca aacaatcaag taagaataat taaaaaattt tgatattgtc tatgtttata
    10561 gttcacaagc cattcaacgt attgtaaact aaggatagtg tattttttta atagtaattt
    10621 gtcaggaggt gcctatctat ggaagaacat tactacgtaa gtattgatat tggatcatca
    10681 agcgtaaaaa caatagtagg cgagaaattt cacaatggta taaatgtgat aggtacagga
    10741 caaacctaca cgagcggtat aaaaaatggt ttaattgatg attttgatat tgcgcgacaa
    10801 gcaatcaaag acacaattaa aaaggcatca atcgcttcgg gtgttgatat taaagaagtt
    10861 ttcctgaaat tacctatcat tggaacggaa gtttatgatg aatcaaatga aatcgacttt
    10921 tatgaggata cagaaatcaa cggttcacat atcgaaaaag tattagaagg tattagagaa
    10981 aaaaatgatg tgcaagaaac agaagtaatt aatgtgttcc cgattcgttt tatagtcgat
    11041 aaagaaaatg aggtttcaga ccctaaagaa ttaattgcca gacattcatt aaaggttgaa
    11101 gcaggcgtaa ttgctattca aaaatcgatt ttaattaata tgattaaatg cgtagaagca
    11161 tgtggtgttg atgtattaga tgtttactct gatgcatata actatggttc aatcctaaca
    11221 gctactgaaa aagagttagg tgcatgtgtc attgatattg gtgaagacgt tacgcaagtt
    11281 gctttttatg aacgcggtga attagtagat gctgattcta tcgaaatggc agggcgtgat
    11341 attacagacg atattgcaca aggattaaac acttcttatg aaactgctga aaaagttaaa
    11401 caccaatatg gtcatgcatt ctatgattct gcttcagatc aagatatctt cactgttgaa
    11461 caggttgata gtgatgaaac agtacagtat actcaaaaag atttgagtga ctttattgaa
    11521 gcgcgtgtag aagaaatatt cttcgaagta tttgatgttt tacaagattt aggattaaca
    11581 aaagtaaatg gtgggtttat tgtaactggt ggatctgcaa acttacttgg cgtaaaagaa
    11641 ttattatcag atatggtaag tgaaaaagtt agaattcaca cgccatcaca aatgggaatt
    11701 agaaaacctg aattttcttc agcaatttct acaatttcta gtagtatcgc ttttgatgag
    11761 ttattagatt atgttacaat taattatcat gataatgaag aaactgaaga agatgttatt
    11821 gatgtgaaag acaaagataa cgaatctaaa ttaggcggat ttgattggtt taaacgtaaa
    11881 acaaacaaaa aagatactca tgaaaatgaa gtagagtcaa cagatgaaga aatttatcaa
    11941 tcagaagata atcatcagga acataaacag aatcatgaac atgttcaaga caaagataaa
    12001 gataaagaag aaagtaaatt caaaaaacta atgaaatctc tatttgaatg attattggcc
    12061 aataaaacta ggaggaaatt taaatgttag aatttgaaca aggatttaat catttagcga
    12121 ctttaaaggt cattggtgta ggtggtggcg gtaacaacgc cgtaaaccga atgattgacc
    12181 acggaatgaa taatgttgaa tttatcgcta tcaacacaga cggtcaagct ttaaacttat
    12241 ctaaagctga atctaaaatc caaatcggtg aaaaattaac acgtggttta ggagcaggag
    12301 ctaatcctga aatcggtaaa aaagctgcag aggaatctcg tgaacaaatt gaagatgcaa
    12361 tccaaggtgc agacatggta tttgttactt ctggtatggg tggcggaact ggtactggtg
    12421 cagcaccagt cgttgctaaa attgcaaaag aaatgggcgc attaactgtt ggtgttgtaa
    12481 ctcgtccatt tagttttgaa ggacgtaaac gtcaaactca agctgctgct ggagtagaag
    12541 ctatgaaagc tgcagtagat acattaatcg ttataccaaa tgaccgttta ttagatatcg
    12601 ttgacaaatc tacgccaatg atggaagcat ttaaagaagc tgacaacgtg ttacgccaag
    12661 gtgtacaagg tatctcagac ttaatcgctg tttctggtga agtaaactta gactttgcag
    12721 acgttaagac aattatgtct aaccaaggtt ctgcattaat gggtattggt gtttcttctg
    12781 gtgaaaatag agcggtagaa gctgctaaaa aagcaatctc ttctccatta cttgaaacat
    12841 ctatcgttgg tgcacaaggt gtgcttatga atattactgg tggcgagtca ttgtcattat
    12901 ttgaagcaca agaggctgct gatattgtcc aagatgctgc agatgaagac gttaatatga
    12961 ttttcggtac agttattaat cctgaattac aagatgagat tgttgtaaca gttattgcaa
    13021 ctggttttga tgacaaacca acatcacatg gtcgtaaatc tggtagcact ggattcggaa
    13081 caagcgtaaa tacttctagc aatgcaactt ctaaagatga atcattcact tcaaattcat
    13141 caaatgcaca agcaactgat agtgtaagtg aaagaacaca tacaactaaa gaagatgata
    13201 ttcctagctt cattagaaat agagaagaaa gacgttcaag aagaacaaga cgttaatcgg
    13261 ttaatatata tacacaaata attcaacaca aatcatcaga taacatatct gatgattttt
    13321 ttactaattt ttagaacatg tagaaggaca tttaagtttt tcaaagttat taaaagtgtt
    13381 taagtatcgt gtgaaaatta agtcaaaaat tatttgcgca acatttt
//
LOCUS       DANJFZ010000069        13400 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.069, whole genome shotgun sequence.
ACCESSION   DANJFZ010000069 DANJFZ010000000
VERSION     DANJFZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.069"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            82..522
                     /locus_tag="PWN45_001758"
     CDS             82..522
                     /locus_tag="PWN45_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDL0623525.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            864..1337
                     /locus_tag="PWN45_001759"
     CDS             864..1337
                     /locus_tag="PWN45_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL0623526.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1312..1998
                     /locus_tag="PWN45_001760"
     CDS             1312..1998
                     /locus_tag="PWN45_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0623527.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            1998..3053
                     /gene="saeS"
                     /locus_tag="PWN45_001761"
     CDS             1998..3053
                     /gene="saeS"
                     /locus_tag="PWN45_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDL0623528.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3125..4108)
                     /gene="csbB"
                     /locus_tag="PWN45_001762"
     CDS             complement(3125..4108)
                     /gene="csbB"
                     /locus_tag="PWN45_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDL0623529.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4240..5079)
                     /locus_tag="PWN45_001763"
     CDS             complement(4240..5079)
                     /locus_tag="PWN45_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0623530.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5301..6650)
                     /locus_tag="PWN45_001764"
     CDS             complement(5301..6650)
                     /locus_tag="PWN45_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0623531.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(6871..8052)
                     /gene="nagA"
                     /locus_tag="PWN45_001765"
     CDS             complement(6871..8052)
                     /gene="nagA"
                     /locus_tag="PWN45_001765"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL0623532.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8360..10318)
                     /locus_tag="PWN45_001766"
     CDS             complement(8360..10318)
                     /locus_tag="PWN45_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0623533.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10324..11244)
                     /gene="pfkB"
                     /locus_tag="PWN45_001767"
     CDS             complement(10324..11244)
                     /gene="pfkB"
                     /locus_tag="PWN45_001767"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0623534.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11241..12005)
                     /locus_tag="PWN45_001768"
     CDS             complement(11241..12005)
                     /locus_tag="PWN45_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0623535.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12255..12737)
                     /gene="ybaK"
                     /locus_tag="PWN45_001769"
     CDS             complement(12255..12737)
                     /gene="ybaK"
                     /locus_tag="PWN45_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL0623536.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(12911..13363)
                     /locus_tag="PWN45_001770"
     CDS             complement(12911..13363)
                     /locus_tag="PWN45_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623537.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         4375 a         2509 c         1866 g         4650 t
ORIGIN      
        1 gaagttaaga attagttaat ggcatattat ttgccttcat tttaaactta acttatcaaa
       61 ttgaagaaat gaggagttag catgaataca aaatattttt tagcagctgg tgctgttatc
      121 acaacattag ctttaggtgc ttgtggtaat tctaattcac aagatcaagg taacaaaact
      181 gaacaaaaaa caaagtcaga agatagcaat gttaaaactg ataaaacaaa acacctaaca
      241 ggtacattca gttctaaaaa cggtgaaact gttgaaggta aagctgagat taaaaatggt
      301 aaattaatgc ttactaacta caaatcatca aaaggtccag atttatacgt ctacctaaca
      361 aaaaatggcg acattaaaaa cggtaaagaa atcgcaatgg ttgactacga taaagaaaaa
      421 caaacatttg atcttaaaaa tgtagattta agcaaatatg atgaagtaac aatctattgt
      481 aaaaaagctc acgtcatctt cggcggcgct aaattaaaat aatcaatttc tgagttaaac
      541 ttttatttac aacatactat tactatacat tacaaatttt taaaatatac attacactca
      601 ttactcaatg gaaagcgtat gatttcacag cccccctagc ttgtagaaat catactttcc
      661 ttttttcaat atatatacaa ctattaaatc ccataagatt gcagagcaca taagtaaatt
      721 tttttagagc ttgaggtttg tttagcttaa gcaacccatg agctcaaaca cttcctgttc
      781 acataacact acaaatcgca ttatgttgct taatcttatg tttatataaa ttacacacaa
      841 taaatagaaa gaatgtgaac atcatgaata aattattgct actcgttaca tttatcattc
      901 gtgtgggttc aggtattgtt atgttaatgc aaggctacga aaaattaacg ggcggattta
      961 cgctgaaagg tttagtacca gtcatcgcta acaatactga ttcaccagag tggtataagt
     1021 ggtttttcgc aaatatagtt gcacatacga cgtcattatt tgatattgtt gtcccactcg
     1081 gagagattgc aattggatta ggtttaattt ttggagtttt tgcatatgct gctagtttct
     1141 ttggagcctt tgttatgata aattatatct tagcagatat gatatttacg tatcctcttc
     1201 aattaacttt ctttatcctt ttactaatga gtcactcatt gttaaaacag atttcactta
     1261 aagaaatcat taattacttt agaggtcgta agaacagagg tgaaaaaata gatgacccac
     1321 ttactgatcg tggatgatga acaagacatt gtagacattt gtcaaaccta ttttgaatat
     1381 gaaggttaca aagtaacaac gacaactagc ggtaaagaag caatttcttt actatcaaat
     1441 gatattgata tcatggtact tgatatcatg atgccagaag ttaatggtta cgacattgtc
     1501 aaagaaatga aaaggcaaaa attagatatc ccctttatct atttaactgc caaaacacaa
     1561 gaacatgata ccatttacgc cttaacttta ggtgcagatg actatgtcaa aaaaccattt
     1621 agtccaaggg aactcgtttt acgtattaat aatttactta caagaatgaa gaaataccat
     1681 catcaaccag ttgaacaact gtcgtttgat gaattaacac ttattaactt aagtaaagtt
     1741 gtgactgtaa atggtcacga agtccctatg cgtattaagg aatttgagtt attgtggtat
     1801 ttagcttcta gagaaaatga agttatttct aaatcagaat tacttgaaaa agtttgggga
     1861 tatgactatt acgaagatgc taataccgtg aatgtccata tacaccgtat tagagaaaaa
     1921 ttagaaaaag agagctttac aacatatacc atcacaactg tatggggatt aggatataaa
     1981 tttgaaagga gccgataatg gtgttatcaa ttagaagtca aatcattatt ggcgtcgtat
     2041 cgagtatact attaacttca actattttag caattgcata tattttaatg tggtttaacg
     2101 gccatatgac actaactttg accttaacga caataattac aagctgttta accttattaa
     2161 tatgtagtat ttttattaat ccacttatac aaaaaattaa gcagtttaat ataaaaacta
     2221 agcaatttgc taacggaaat tacgcaagca atgataaaac gtttaattca ccaaaagaaa
     2281 tttatgaatt aaatcaatct tttaataaaa tggcttctga aattacgcaa caaatgaatc
     2341 aaattaaatc cgaacaacaa gaaaaaacag aactgattca aaacttagcc catgatttaa
     2401 aaacaccttt agcaagcatt atttcatatt ctgaaggact acgtgatggt ataatcacta
     2461 aggatcatga gattaaagag tcatacgaca tattaattaa acaagcaaac agattatcaa
     2521 cattatttga tgatatgact catattatca ctttaaatac aggtaaaaca tatcccccag
     2581 aattaataca actagaccaa ttacttgtat caatattgca accatatgag caacgtatca
     2641 aacatgaaaa ccgcacatta gaagtgaatt tctgtaacga aattgatgca ttttatcaat
     2701 atcgaacgcc acttgagcgt attttaacaa acttacttga taatgcgcta aaattttcaa
     2761 atgttggtag tcgcattgat attaatatta gtgaaaacga agatcaagat actatcgaca
     2821 ttgctattag cgatgaaggt attggcatta taccagaact acaagaacgt atattcgaac
     2881 gtacattcag agtagaaaac tctcgtaata caaaaacggg tggttctgga ttaggcttat
     2941 atatagctaa tgaactcgcg caacaaaata acgcaaaaat cagtgtaagc agtgatatag
     3001 atgtaggaac tacgatgact gtaacattac acaaattaga cattacgtca taatccgatt
     3061 tatttataaa ataaaatgca aagactaaaa agaagctccc attaatgagg gcttcttttt
     3121 ttgtttattt agaataaact ttatgggtat ccttctcatc attttcaaga cttgaaagat
     3181 ttgtagcttg aataatatat ttaggacgtg ccttaacttc ataatatatc ctgccaatat
     3241 attcacctac aacaccaatt gaaattaact gtatgccgcc taataataaa atagctgcaa
     3301 tcgttgaaaa atatcccgga atattaacac cagatatcat aatattgatg aataaataga
     3361 taatatataa cacgcttatt gaaaagataa acaagccaag ataaatcatc attctcaaag
     3421 gtttactatt aaaggaaatc aatccatcaa taccataatt aaataacttt ttaaaggacc
     3481 acttagattc ccctttttgt ctctcaacat tttgatacgt aaacacttta gtattatagc
     3541 ctatccattc aaataaccct tttgaaaatc gattatattc ttcaagtgat gcaatggatt
     3601 taacagctct ttggcttaaa agtctaaaat caccaacacc atcatcaaat tgtacttctt
     3661 ctacaaagca attaactaac ttataataca aatggcttaa tgttttgcga ctaaaatttt
     3721 caccacttct atcacgcttt gcaatcactt gatcatagcc ttccataaaa ccttcaacca
     3781 ttttcggaat atattcaggt ggatgttgca aatcacaatc tatcatgatg actgcatcaa
     3841 attcagtact atgctggtaa cctgcaatca tagctgcttc ttttccaaaa tttctactaa
     3901 aagaaataaa gctgacatgc ctatctattg tggcaagatg ttgcatttca tctatagtgg
     3961 tatccgtact accatcatct atgaaaagca tattataatc atagcctttc acactactat
     4021 cttgtgaaag tatttcagtt aattgttgat gtgtttgtgt aatgacttcc ccttcattaa
     4081 aacaaggaat gacgactcta attttcatct atgtccatcc tttttattta aaagattttg
     4141 aatgtacctg agtattgttt tatcaaatag taaaaaccaa ctcatttatt ttaatgtaca
     4201 cattaaaagg cattcataaa cgtaaaattt aacatccagt tattttaaat ccccaatttt
     4261 cacatcatct ggatttttac cttgtcttgc atttctattt aaagcatcaa tttcagctac
     4321 ttctgttaat tctaaattaa aatctaaaat atctatattt tcttgaatgc gtttaggtgt
     4381 ctgagatttt ggaataataa ttctattgtg tgctaaatgc caacgtaata caacttgtgc
     4441 tggtgtttta tgatattttt cagcaatttt aacaatgaca gggtcgtcta gtagtcctct
     4501 atttctcatc aaaggcatcc atgcagtcac tttaatatcg tgacgatcac aaaattcttg
     4561 taattcttgt tggttaaaat atggatgtac ctcaatttga ttcaccattg gtttgatact
     4621 tgattgagcc attaattttt ctagatgatg aacattaaaa ttacatacac ctattgcttt
     4681 taccttacct tgctcgtaaa gttcttccat agctttatat gtttctaaaa atagaccatc
     4741 tgcttcacaa ggccaatgta ttagaaataa atcaagataa tcagtttgta aattttcaat
     4801 cgatttgttg aaatattcga atgttttctc ataaccttga tagtcattcc ataacttcgt
     4861 tgttataaac aaatcttctc tatcgacgcc attatccttt aatgctcgtc ctagtgaagc
     4921 ctcattatca taaaagtatg ctgtatcaaa cgctctatag cctgcgtcaa ttgcagcatt
     4981 tacaacttta gtcatatctt cgtcagagat tttataaaca cctaacccaa ctgaaggcat
     5041 cgggtatcca ttatttaata tttgtatctc attcaacatc tttatatctc tccaatctat
     5101 gtatctttta tatctttaca ttaccctaaa tttttcaaca aactcaatta atacgaatta
     5161 tcgctttcaa taaaaattat tcattaaatc attaaagata ttgagttcca atactatttt
     5221 cacttttcat taattttagt caaaaaaata accaaccaaa aatgaattaa atcattctta
     5281 gttggtttat atattaatat ctattctgat ttttcatctt catcagactg tccgatagta
     5341 ggtctcgctt cattaaattc atagtttaat atcacccaaa taatttggtg gttatcgatt
     5401 tctgaaacaa cccagcgatc ataagttgta tccacgtaat catctttttg taaattggta
     5461 ttacgagatt gtaaccatcc acctatcgta tcaatatcct cagagtcatc aaattctata
     5521 ccgaactctt cagttaaatc atccaatagt actctgccat ttacttggaa tgtcttatta
     5581 tcaattttaa cgatatcatt cacttcatca tcatcaaatt catcacgaat ttctccaacg
     5641 atttcttcta aaatatcttc catcgttaaa atacctgccg ttccaccata ttcatctata
     5701 ataagactca tatgtacatg ttcacgttgc attctaatta atgcatcact gatacgtgtt
     5761 gtctctgaaa tcattggcaa ctcatgtata tagtttgcta ttttaatcgt ttttccagaa
     5821 gcgtattcag ttaaaaattc tttgacgtta ataaatcctt taatgtggtc tttatcacca
     5881 tcatcagtaa ttggataacg cgtaaattga tgttctttta ttgtttctag taattcgtct
     5941 acattaaaag gttcatttag tgtaatcatt tgagttctag gtaccattat atcttttgca
     6001 tgtctttcat cgaatgaaaa gatattttgc atatatgcca attcagtttg gttgatttct
     6061 ccaccattat aactattgtt aataataatt ttgatttctt cttctgacat tgcatcagtt
     6121 tgggcatcag gatttacacc aaacattcta ataataacac gtgcagaacc attcatcagc
     6181 caaatcaatg gtttcataat gttaccgaaa tagaacaatg gtcttgcata tactaaagca
     6241 agcttttcag tatgttgaat agctatagat ttaggcgcta attcaccaag tactacatgc
     6301 aaatacgtaa cgattataaa tgacactgca aacgaaatcg tcgtcgttaa tgcagttggt
     6361 aaattgattg cttcaaatat tgggtgtaat agcttttcaa acgttggttc accaagccaa
     6421 cctaacccta aagatgttac tgttatacct aactgacaag cagaaagata ataatctaga
     6481 ttagcaatca tcttttttac tattttagca ggtttatttc cttcatctgc tagctgttca
     6541 attcttgttg ctctaatttt tactaatgca aattctgaac caacaaatac agtggttaat
     6601 gcaattaata gaataaatat aatcaaacta attatggtcg aagtttccaa ttaattccct
     6661 atttctaggg attcacctcc atgttatatg tgtcactcat gggtaacacg cattcaaatt
     6721 tatcactatg acttaaatta caactacatg ttatcgcctt cccatttaga cacccccaga
     6781 aaaaaatgtt attgcttcta ttttatcata atataaagtg cttatgttaa cagattaaat
     6841 ctattgcata catttaatta tgattattta ttagctaaat gtgtgaacct tgccttgttt
     6901 tattgtagat tttacattca tatcatcatc tagaataaca agatctgcat ccttatttac
     6961 tttaatacta ccttttctat catcgatacc taatgcaatg gcttgattta aacttgttac
     7021 tcgccataaa tgatctaatg tatcacctgt aaatgatatt aagttacgta acccatgatt
     7081 catttttaaa atactaccag caagcgcacc atttgcaaga cgtgcttgtt gcgattgaac
     7141 agttactttt tgtccaccca aatcatattc tccttcaggc ataccttttg cacgcattgc
     7201 atcggtaatt aaataaaaac gttcattacc tttcatacgg taagcaattg caaccgatgc
     7261 cggatgagaa tgagtgccat caacaatcat ttcggtatgt agagcatcat tcaaccatgc
     7321 tgctccaaaa acacctggtt ctctatgttg gaatggcgtc gctgcattat ataaatgcgt
     7381 gacatgttta gctcctcgct caacagcttc aactgcttct tcgtatgttg ctactgtatg
     7441 accaattgaa aaaataatgt catctttata cgtttcaagc gcttcttttg caccttcaac
     7501 ttcaggtgca aacgtcataa tctttattaa tccgttagca gtctcttgaa aatgtttaat
     7561 tttatcgata aatgggcgta caacgtattg cggatgttga gcaccaactt tattttcaga
     7621 tataaatggt ccttctaaat gtatacctac aatttccgct gcattgtgaa catcttgctc
     7681 cgcttcatat ttagcaatat ttgtaagtgc attatctatt ttatcagtcg attgcgtcat
     7741 tgtagtggcc aagtatgatg tcgtcccttc agacaacaaa ttttcggata gatattttaa
     7801 gccatcgtat gacccatcca ttgcatcttg accataacca ccatgaatat gtatatcaat
     7861 aaaacctggt aatacatgat gacctttagc atcaatcact tgaattttat ttgtcgtatc
     7921 attatcaatt gctgctttat catccacttc tccaattgca acaatctgtc catctttcac
     7981 atgaatgtaa ccattatcga ttttgccatc ttcagtataa actttgccgt tatatataat
     8041 taattctgac acgtataaac ccttctttat ttataaattt tcactttaag acactcatat
     8101 tttactatat ctgcttcgtt tggcataact ttttgcaaaa tattaaaaaa gtctatacat
     8161 ttgtggtgtc tatttcttca ttcatttatc taaaaataaa aacactttca tatcaattca
     8221 taaaaactga attaatagaa agtgttttta cagctttgta taagctaccg ttaacacgat
     8281 atataaaact aacatatatt cattgaacat acaacttaat atgaagctct ttgctaacaa
     8341 tcattttaca tcattaaaac tactcgtcca ttgattttga agcttcgatt tctgtttcag
     8401 ttaactttgg tttgattaaa ccgtaaatta atgctgaaac taatgtgcca actagaagtg
     8461 caataagagt ttgaagtaag tgtgcaccat cagtaccaac aattacaata ataccaccat
     8521 gtggcgcagt aattcgtgaa cctaagccta aagcaattgc gccacctata cctgaaccaa
     8581 tcatcattga aggaataaca cgtaatggat cggcagctgc aaatggaatc gcaccttctg
     8641 taataaatga catacccatc acatagttag ggataattga accacgttgt tcttttgtaa
     8701 atttacgtct aaaaattaac atcgctgtcg caattgctaa cggtggaatc ataccaccaa
     8761 tcattgcagc tgtaattggt gctgcattac cttcaatcaa cgcacctgtt gcaaaaacat
     8821 atgccgcttt gttgaatgga ccgcccatat caatcgccat catagcgcca ataactaaac
     8881 ctaataatac aatattagaa cctgataaat tgtttaatcc atctaataac aaatgattta
     8941 accaagatgc tggtggatta aaggcataaa tcatcaataa gcctgtagcc gtcactgtta
     9001 atagtggata aattaatgtt ggctttaatc cctctaatgc ttgtggtaac ttacgtgtca
     9061 tggctttaac accttgtgtt aagtaacctg ctaagaaacc tgcaataata ccaccaataa
     9121 atcctgaacc acctgaaatt gctaacatac cacctacaag acctgaagcg aaaccaggtt
     9181 tatccgcaat actacgtgca atgaatccag ataaaattgg aatgattaac gcgaatgcac
     9241 ttttactacc aatgttccaa agctgctctg caaacgcatt gtactctgag ctttttggat
     9301 taaatgaatt tgctccaaat aaaaatacaa ttgccattaa aataccacca gatattacaa
     9361 gtggcaacat gttagaaaca ccgttcatta agtgtttata gaatgcttta cctgggctta
     9421 atttttcttg actgtcattt gagttacctt ttctttgacc atcacgggca acaaaaggtt
     9481 tacgacttgt atctaatgct ttattaatta attcttctgg gcgtttaata ccatctgcta
     9541 ctggtacttc tacgacattt ttaccatcga agcgatccgt ctcaacatga acatcagcag
     9601 caacaatgat acctgttgca ttttcaatat cttgttcagt taaatggttt ttaatgccgc
     9661 ttgaaccatt cgtttctact ttaattttaa tacccatttt atccgcttgc tttttcaatg
     9721 catcacgtgc catatatgtg tgtgcaatac ctgttggaca agcagttact gctaacacat
     9781 atggctcatt agattgttta gatgattgtt ctgcagttgc aacttgttgt gcttcagctt
     9841 ctgcctcttc ttcttttgtc acttcatcat cagcctcatc tatgatcgct agtacttctt
     9901 caggtgatga agcatgtaat aatttctcac gtacattttc atccattaaa ataccagaca
     9961 acttagctaa agcatctaga tgtgtttggg cgccaccttc tggcgctgca atcataaaga
    10021 ataagtgtgc tggttgcata tccaaacttt gataatctac gcctgcttta gatttaccaa
    10081 acgcaatagc tggtgactta actgcggcca ctttggcatg tggaatggca ataccttcgc
    10141 cgataccagt tgtactttgt gattctcgat tgtgaatcgc ttccttaaat gacgcgacat
    10201 cacttaattt acctgctttg tctaattgat ttactaactc atcaataaca ccatttttgt
    10261 catttgccat taaatccatt gctattgtat cttttgttaa taactctgtt actctcatta
    10321 ttttcactcc ccatcaagta cgctaatcgt aacttgtgat tttatttttt ctatagcgtc
    10381 ccgtgttgct aagtcctcat caaatgccgt ggcagtaccg catgcgactg cttgttggaa
    10441 tgctttttca atcgttaaac ctgaagcaat tccagccacc atgcctgcaa ctgtactatc
    10501 accagagcca actgtattaa ccactttccc ttgtggatta actgctttaa tactgatttc
    10561 tttatcaata taaatagcac catcaccgcc aagcgagaca ataacagatt gcgcaccttt
    10621 atcaactaac aaacgaccat atttaataac atctgtgtct gagttcactg ttgtattaaa
    10681 catcacttct aattcatctt tattaggttt aataaatagt ggatgatatg gtaaaacgct
    10741 ttcagccaat tctttttcag cgtcgactac taatttagca cctgtctgtg ctgtaatttg
    10801 tgcaatttgc gcatacgcat cgcttggaat actacttggt acacttccag caacaataac
    10861 tatatcttcg cttgttgtat ttttaatttg ttgtaacagt tgttcaaatt gtgttgacgt
    10921 tatatgagga cccggtgcat tgatttctgt ttcttgtcct gtttttaatt tcacattaat
    10981 acgtgtatct tcatcaactt caataaaatt cgattgaatt gcactgttat ttaatgtatc
    11041 tataatgaat ttcccaggaa atccacctgc aaatcccaag gcagttgact caacatccaa
    11101 tgtctttaag acgcgcgaga cattaatacc tttcccccca gcgaatttat atgttgctgt
    11161 tgctctgttc aaaccatcaa ttttaaaatc attcgtaaaa atgacatagt caattgaagg
    11221 attgaaagtc actgtataaa tcataaagtc cctcctataa agtgatactt ttgttggtat
    11281 tcttttaacg attcttgatt taatgctttt tcagatgtga tgattgtcgt actttctagc
    11341 aaaggtacac gagcaaaata tactttatta aacttagaat gatctataag tacaaatgat
    11401 tgattggcta atgacattgc tgtttgttta actaatgcct cttgctcatc gggagtagtt
    11461 aatccaagtt caatatctaa tccattcatc ccgataaaag ctttatcgaa acaatatcgt
    11521 cttaatatct ccatagcact agaaccaatc gtagcaagtg tattttcttt aacttgacca
    11581 cctagcataa ttgttttaat acctttttta agtaaagctt ctacatgtgt taaaccatta
    11641 gttaccacaa tgatatcttt cgcttgaata tatttaatta gctccaatgt agatgaacca
    11701 gcatcgataa ataagcattc attatcgttg atttgattag ctgctatttt agcaatcatt
    11761 ttcttttcat caagattcgt tgctaatttt tcagttaaat tcgcctcaac catacgattt
    11821 tcttttaaca ttgcaccacc atgcacacgt tgcagtttcc ctaattgttg tagtttagat
    11881 aaatctcttc gtattgttga agcactgcaa ccagttcgat ctattaattc ttgtaaagtc
    11941 aaaaaatctt tgtgcgaaag ttcttctaat attaactcgt gtcttttttc agtaattatc
    12001 atcatcaccc catctcactt tgtattataa tgagtacgct ttcacttttc aatcataaat
    12061 taccaaaaac aatcacaaac actcaaattg tgacgataaa attacatttt tatgttcaaa
    12121 tactcaatta taatgactgt ttatgatcgt aaaatgctaa ttcaatcaaa aaggagtgtg
    12181 gcagaaatga attttataaa aatttcatct ctgtcacact cctaatcttg ccccatactt
    12241 tttgttgtta ttaatcattc atggataact gctccaattt tgcctttagt tattgtaatc
    12301 aaatcctcaa cagctattgt gatttgcatt gttcgaagcc caccactcac actgatatga
    12361 ctataatttt cacacgattt gtcaacgact gttggaaaca atgttttcat accaacaggc
    12421 gaacacccac cacgaatgta tcccgttaca tttttcaaat tatctaaagg cataagctgc
    12481 aatttcttct ctccaaccaa agcagctgcc tttttcatat ctaaagtttc acttactggg
    12541 ataacaaata caaaatgttc atgttttgta ttttctagaa caagcgtttt aaatactgtt
    12601 ttaacatttg cttgtatgag ttctgcgact tcttgaccag ataaatgttc ttctggtacc
    12661 tcaaagctat gaacttcgta tttaattttt gcacgatcaa gcatacgcat cgcgttcgtt
    12721 ttcttatttt tagccatttc aacacctcat ttatacttct tcagaactat attaacattt
    12781 taaaatacat tacggattgg ttgattttga cataacattt acatcatttt ataagttgaa
    12841 ttcacctttt taaattaaat tattgcaaaa taaaaacagt atatccaatt agaatacact
    12901 gttccatcta ttatttcgtt attatgcctt gatttgaaat acttacatgt tgcttactag
    12961 ttgaattatc attggaatac acataattat atccatgatt tgtcttagaa attctttcta
    13021 aaccataatt atcattgata tgcttatctg cataacgatc agcaataact tggcactctt
    13081 gttcgttaaa agaatttgat ttatttgcat tcacacgatg tttaaaatga ttttgctttt
    13141 tcacattttc attcacttca gatgctttag cagtatgaat attgagaata tccattttct
    13201 ttttataagt atcttcagtt gatacattta tcgcgttaag cgtattaatt gttttatgta
    13261 ccttagcaag cgcatcttca tttgttgcta atattaataa accaattaaa caaacggtta
    13321 atattacagc aacaaagcct aaaaataaat tttttactgt catcccctta cccacatttc
    13381 cttactcatt ttttatcatc
//
LOCUS       DANJFZ010000070        13343 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.070, whole genome shotgun sequence.
ACCESSION   DANJFZ010000070 DANJFZ010000000
VERSION     DANJFZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.070"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            54..749
                     /locus_tag="PWN45_001771"
     CDS             54..749
                     /locus_tag="PWN45_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0623538.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            751..1521
                     /locus_tag="PWN45_001772"
     CDS             751..1521
                     /locus_tag="PWN45_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623539.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1894..3858
                     /locus_tag="PWN45_001773"
     CDS             1894..3858
                     /locus_tag="PWN45_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDL0623540.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            4202..5281
                     /locus_tag="PWN45_001774"
     CDS             4202..5281
                     /locus_tag="PWN45_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623541.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5386..5979)
                     /locus_tag="PWN45_001775"
     CDS             complement(5386..5979)
                     /locus_tag="PWN45_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623542.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(6001..6969)
                     /locus_tag="PWN45_001776"
     CDS             complement(6001..6969)
                     /locus_tag="PWN45_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL0623543.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(7047..7481)
                     /locus_tag="PWN45_001777"
     CDS             complement(7047..7481)
                     /locus_tag="PWN45_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0623544.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7708..7992
                     /locus_tag="PWN45_001778"
     CDS             7708..7992
                     /locus_tag="PWN45_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0623545.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8163..8969
                     /locus_tag="PWN45_001779"
     CDS             8163..8969
                     /locus_tag="PWN45_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0623546.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9089..9760)
                     /locus_tag="PWN45_001780"
     CDS             complement(9089..9760)
                     /locus_tag="PWN45_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0623547.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10030..11022
                     /locus_tag="PWN45_001781"
     CDS             10030..11022
                     /locus_tag="PWN45_001781"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL0623548.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(11271..12071)
                     /locus_tag="PWN45_001782"
     CDS             complement(11271..12071)
                     /locus_tag="PWN45_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0623549.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            12392..13027
                     /locus_tag="PWN45_001783"
     CDS             12392..13027
                     /locus_tag="PWN45_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0623550.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            13033..>13343
                     /locus_tag="PWN45_001784"
     CDS             13033..>13343
                     /locus_tag="PWN45_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDL0623551.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     L"
BASE COUNT         4412 a         2322 c         2044 g         4565 t
ORIGIN      
        1 ttatatataa aatcttaaga ttcagattat ttaatagcaa aggagatagt gatatggatg
       61 ttttaacaat agaacattta acaaagaaga taggcaacaa aacgattctc gaagatgtat
      121 catttaagct gaaacgcgga caaatagttg gtctcgttgg agcgaatggt gcaggtaaaa
      181 caactttaat gaaagttata ttaggttact ctagtttcca aagcgggaat tttaatgtta
      241 ttaacagcaa ggacagcaaa agcaatatcg gcgcattgat tgaaaatcca ggaatatatc
      301 cttttatgtc tggatatgaa aacttgaagt tattgaatga atcaaaaaac actcaagata
      361 tcgataaaat tgtctcacaa cttcatatgg atgaatacat tcataaaaaa gctaaaacgt
      421 attctcttgg tatgaaacaa aaattaggaa ttgctatagc atttttaaat aaacctcaat
      481 tcattatctt agatgaacca atgaatggct tagatccaaa agctgtgcga gatgtacgtg
      541 aattgattgt ccaaaaagcg caagaaggtg ttactttctt aatttcgagt catattttaa
      601 gtgaattagt taaaatcaca aactctatcc ttattattaa caaaggtaaa attgttacag
      661 aaacatcgga agaagaactt aaacaattta aagataatga tttagaaaat gtattactag
      721 aaatcataga aagggaggac caagcataaa atgggaactt taattaaaca agaatgtttc
      781 aaattattta aaaagaaatc aacttttatc gcacctattg tctttattct actaatggtt
      841 gctcaaggtt atattgctac aaaatacaat gaaattttta cgccacagga atctttcaca
      901 tctgcttata atggtttttc atggtttgca tttttattaa ttattcaagc aagtacaatc
      961 atttcaatgg aatttcatta cggtacgatt aaaaatttac tctatcgtga atattcaaga
     1021 acaactatga ttgttagcaa aatcatcaca ttatttatta tttctttaat ttattttgtt
     1081 attacaatta ttgcttcaat tgttattggg tctttattct ttaatgattt aaatatattt
     1141 gaaagtagcg gtaatcaatt atctttattg aatcaattat tattagttag tttaggcaca
     1201 tttgttggcg tttggttagt tttaagctta acgttgctat tatcatctgc aacaaattca
     1261 acgggagtag ccattgctgt aggtattgtt ttttattttg caagttctat tttagcagtt
     1321 attcaaacgg cacttttaga aaaaatagac tggctaaagt ggaatcctat taatatgatg
     1381 aatattatgc ttcaaacagt tgaaaaaggc tttagtaagt cgacaaaatt agaacttcat
     1441 gaattgttta ttggtaatat tgcttatatt tctattttct taatacttgt agtatttatt
     1501 ttcaagaaga aaaatattta gtaacttaaa gtattaaatg tctaaataca cacatattcc
     1561 atcgtaattc aaaatcattt tcaaatccct tcacccaaat aatggtgcgg ggattttttc
     1621 atccaaattt tggaaattcc acattttaca tatcgtaatt ttttaggaaa ctagtgaata
     1681 taacaaatcc ctcctctcat ttttaaaata gatatatcac ttccccactt ttacttaact
     1741 aaactgcaac ggttcctaat accaaaatcc tgccctctat ttttatcaat tcaagcatac
     1801 ttattgaaaa atgttaacgt tttcttgata atcattgtaa gcgcatttat tttataaact
     1861 aacgtttgaa atatactaca ggagtgacac gtaatgactc aaattactga aaaagaatta
     1921 aaaaagaagt atttagattt actatcccaa aattttgata ctccagaaaa acttgcaact
     1981 gaaattatca atttagaatc aattttagaa ttacctaaag gtacggaaca tttcgtcagc
     2041 gatttacatg gtgaatatga agctttccaa cacgtattac gcaacggttc tgggaacgtg
     2101 cgagcgaaaa tcaatgatat tttcaaagag agactttcaa ctaaggagct taatgactta
     2161 actgctcttg tctactatcc agaagacaaa ttaaaattga ttaaaagtga tttccaaaat
     2221 tgcggtcaac ttaatgtctg gtatatcaca acaatcgaac atttaattga gttaattaaa
     2281 tattgttctt caaaatatac gcgttcaaaa ctgcgtaaag cattgccaaa acaatacgtt
     2341 tatattattg aggaactact gtataaaagt aatgaatatc aaaataaaaa atcatattac
     2401 gaaacacttg ttaaccaagt aattgaactt aaacaggcag atgatttaat tattggactc
     2461 gcttattccg tacaacgctt agtcgtcgat catttacacg ttgtcggtga tatttatgat
     2521 cgtggaccac aaccagataa aattatggat acactgatta attatcattc cctagatatt
     2581 caatggggta atcatgatgt gctttgggtt ggagcctatg ctgggtcaaa agtatgctta
     2641 gcaaacttac ttcgaatttg tgcacgttat gataatttag atattatcga agacgcttat
     2701 ggcattaatt taagaccact gcttacttta gctgaaaaat actatgacgc agataatcct
     2761 gcttttaagc ctaaaaaaag acctgacaaa cacgaacgtt taactcaacg tgaagaaagt
     2821 caaattacta aaattcatca agctattgcg atgattcaat tcaagttaga aataccaatt
     2881 attaaacgtc gtccaaattt cgaaatggaa gaacgtcttg tgcttgaaaa ggttaattat
     2941 gatacaaatg aaattacagt ttatggtaat acatacccat tgaaagacac atgtttccaa
     3001 actgtcaatc gtgataatcc agcagaatta ctacctgaag aagaagaagt catgaataaa
     3061 ctattattgt cattccaaca atctgaaaaa ttacgtcgtc atatgtcttt cttgatgcgt
     3121 aaaggctctc tttacttacc atataatggc aatttactca ttcatggttg tattccagtt
     3181 gatgaaaatg gtgagatgga atcatttgaa attgatggtc atacttacag cggccaagaa
     3241 ttattagatg tgtttgagta tcatgtccgt aaatcatttg atgaaaaaga aaatactgat
     3301 gacttatcga cggatttagt ttggtattta tggactggga aatattcgtc actatttggt
     3361 aaacgtgcca tgactacgtt tgagcgatac tttattgcag ataaagcttc tcataaagaa
     3421 gaaaagaatc cgtactatca tcttcgtgaa gatgtgaata tggttcgtaa aatgctcagt
     3481 gatttcggat taaatccaga tgaaggacgc attattaatg gtcacacacc agtgaaagaa
     3541 atcaatggcg aagatcctat caaggctgat ggaaagatgc ttgtcattga tggtggcttt
     3601 tcaaaagctt atcagtcaac aactggcatt gcaggctata cactattgta taattcattc
     3661 ggtatgcaac tcgttgcaca ccaacaattt aacgctaaag aaaaaatact ttccgaaggt
     3721 atcgatgaac tttctataaa gcgcgtcgta gataaagaat tacaacgtaa gaagattcgt
     3781 gataccaata ttggtaaaga tcttcaagct caaatagata ttttgaaaat gttaatgcat
     3841 gatcgttact tagactaaag acgacatttg atgcatgtat cgtgcactta tttagtggta
     3901 ctgtgtttac gcattggcta cgcaaacacg atgataaata ggcgacctag ctacaccgaa
     3961 aaaaatcccc tcactattgc gagtagtgag gggattggtg tatctatgct aattcctgtt
     4021 aactgtatta taacaacttt acagcaaaaa aatgcaaaat caactaataa attaatgaaa
     4081 tgaccgacac cctcgcttct actccaattt aacaaaatac tatattagta ctatattttt
     4141 cattaacatg tatgttacat tttctatatt acttaaaatt acgaagggac atttttaaat
     4201 catgcgtatc ttaaaagagt ccattattgt ggcatttgcc tttgttggtg ttgtcgttgg
     4261 tgccggcttt gctactggtc aagaaatttt ccagtttttc acaagtcatg gcgcatatag
     4321 catttcaggc attattgtaa caggactatt gattacttta ggtggaatgg ttgtcatgca
     4381 tacaggtcat catctaaagt ccagaaatca ttctgattca attaactatt tcttataccc
     4441 ctctattgca agaggttttg atattatttt aacaatgttt atgttgtctt tagctattat
     4501 tatgactgca ggtggtgcgt caaccattca tcaaagtttc aacttaccgt attggctgag
     4561 cgcactcata ttagtcgcct ttattttagc aacactgttt ctaaaattcg atcgtttaat
     4621 tgctgtgctt ggcggtgtta ccccattttt aattgcgatt gtcattatga ttgcggtcta
     4681 ctatttcaca acaagtcatc ttgattttac tgccgctaat aatgatgctc agattcataa
     4741 gcagaaatca ttatcacctg gatggtggtt tgatgcgatt aactatgcaa gcttgcaaat
     4801 tgctgctgcc ttcagcttct tatcagtgat gggtagtaaa gttaaatatc gtgactcaac
     4861 gttatacggg ggcttgattg gcggtttaat cattacattt ttactcatga tgattaatct
     4921 aggtttaatt tctcaattcg ataaaattaa acacgtagat ctacctacat taaaattagc
     4981 gacacaaatg tctccgtcaa ttggtattat tatgtctgtc attatgatac ttgtcatcta
     5041 caatactgtt gttggattaa tgtatgcatt tgcgtcacgt ttcagcgttc cattcagcag
     5101 acgttacttc atcattatta ttacaatggc tgtcatcact tatattagta catttatcgg
     5161 tttcatttca ttaattggaa aagtattccc tattatggga ttgttcggtt tcatcttact
     5221 catacctgta ctctataaag gtttaattaa gcgtattacc ggcaaatctc atatcgatta
     5281 aaatatctat actattgtaa aatttagtac agcacttcaa aatcaaaaaa gcgggcagat
     5341 gaaaagtaaa aatcttttcc atctgtccgc ttcttaatag aactgttatt tcccaagtat
     5401 ttgttgacca gccgttaatc cagtttctgt aatttcatgg cccttcaccc aaacttcttc
     5461 gacttgtgcc ccacgtgtat taaacaagtt aatgacttgt tcacttgcag ctaatggcac
     5521 aattggatca tgtttcccca tagaaagcaa cacactgaca tctgataaat cctttgttga
     5581 cgttacttca actgggtata acggtgcata taacaatgct tttttaaatg gtgcttctga
     5641 acgcaacatt aagttaatcg ctatatttga tccatttgaa aatccaacaa gtactgcttt
     5701 ttcaatatca aaatcataac gttcagcagc ttctttaatg aacgtcaaca attcttgtcc
     5761 acgaaatgcc aaatcttctt catcataaac accttcacca agacgtttga aataacggtt
     5821 catcccattt tctgaaactt gtcctctaat acttaacaag tgataatttt cattcaatgc
     5881 ttcgcctaac ggtaataaat cgaactcatc accacctgta ccatgcaata atattagtgt
     5941 tggcgcacca ttttgtcctt ctctaaaaat atgttccatc acaaatcctc ctcattccaa
     6001 ttaaccatgt tgacgcttcg tattaaaagg tctaacttcc gattcaatat attctctttt
     6061 attttctaaa aatggtggta aggataaccc ttcgcctaat gtttcataag gttcatcttc
     6121 cataaatcct ggtccatctg ttgaaatttc tattaaaata tgccccacac gtgcatataa
     6181 tgcttcaaaa tagaaacgat taacgatgcc tgagttatta atacctacct ctttatattt
     6241 cgttgcccac gcttctattg catcatgatc tttcacacga aatgacacat gatgtacctc
     6301 accataacct tgacgtgctg ctggcccttt atcatctttt attaagatta cctggccacc
     6361 attgcctcct tcgccaactt caagtaatgc gacattatct tcatgcgcaa tagttgtcat
     6421 accgtaaaca gtctctaaaa tatttttaaa gtcgtcaaaa taacttactt taatttcaat
     6481 ggggcctaat ccataaatcg ctttatctac tggaaccggt ccattcttcc aaggtacacc
     6541 aggtgctacc ccttcattta actcatctga aattaattga tacacttggc catcgacttc
     6601 ttcaaatggc aacacttttt taccaaataa ttcttgaata ccttcgtgtt taacaccaaa
     6661 ctcatcaaag cgctgttcat aatatgttaa tgcgtcatcg ttaggcactc taaaagacgg
     6721 tcttgtaatg gaatttgttc ctgcctgccc ttttgtaata tttggaaaat caaagaacgt
     6781 catgtctgta cctgccgaac ctacatcatc tgcaaaaaaa gtatgatacg tataaatatc
     6841 atcttgattg actgtctttt taactaaacg catgcctagt acttctgtaa aaaatttata
     6901 attacgttct gcatcatctg tcattgcagt aacatggtga atacctagta attcatgatt
     6961 atttatcatt ttatataccc tctttatatt tttagtgcat ttatctcgaa atcgagatac
     7021 tttaatgtaa atttttagtg cacgccttac acttctgtag attgtgcact tagtttctta
     7081 aacgcttgtt gtaagattgt taattcatcc tttgttaaca catcaaacgc ttttgttaat
     7141 gtctcagcat gcttagggaa aatatctgcc atttgacttt gacctttttc agttaaacaa
     7201 gccatatata cacgtttatc atctttatcc ttttcacgtg taatccaacc tttgtcctct
     7261 aattgactta caacatatga aatgctgcta cttgcaatta atacgcggtc tctaatacgt
     7321 tgaattggtt gcggaccttt attataaagc aactcgagca ctgcaaattc agtaatattt
     7381 aagccatatc gctttacgtc ttcttttgta atttgctcta atgtgtctaa cgccctattc
     7441 attccgacaa aaacattgag agattgtttc gttcgatcca tatgatcacc aactttttta
     7501 ttttatttcg atttcgagat aaattatata gctgaataga tttaatgtca agatacgtgt
     7561 tttcatcttc atttcaaact gtgcttaata aaaacgacta catagatgtg gcaatgtcgt
     7621 cacatgactg tcatgcacct ttgacttcgt ttaagttaaa atagaatagg gaaatactat
     7681 aatcaatcat gataggagtt gatatgtatg agtaaccttg aaatcaaaca aggcgagaac
     7741 aaattctata ttggtgatga tgaaaataat gctttagctg aaatcacata ccgttttgtg
     7801 gataataatg aaattaacat tgatcataca ggcgtatctg atgaacttgg tggtcaaggt
     7861 gttggcaaaa aactacttaa agcagttgtt gaacacgctc gagaaaataa tttgaaaatt
     7921 attgcctcat gttcatttgc caaacatatg ttagaaaaag aagattcata tcaagatgta
     7981 tatcttggtt aaataaattt tgcgatggtg caactgtacc atcgcttttt tgttcgcatc
     8041 aatataaaat tgcaaaacga ctagttattt tgcatttcaa actttatgct atcaattcat
     8101 tttcttttct ggtatacttt ttatactaac ttatagaaaa atagaaaaag aggcgatgca
     8161 taatggcatt tcacgataaa acagcaacac aagtgacaaa cattgtactg aacgtaagag
     8221 acttagactt aatgacaaca ttttacaaaa acattttagg actatccgtt aaaagttctg
     8281 acgataatac aaccgtatta tctgttggga ctggtggcca tactctaacg ttacatttat
     8341 tagaagacgg ccgtcagact tccccacgtg aagcagggct ttttcatata gcatttttat
     8401 taccaactac tgaggatcta gctaacttct tatatttcgt ggcacaaaaa aatatgggca
     8461 tcggcgctgg tgatcattta gtaagtgaag ctttatattt caacgacccc gaaggtaatg
     8521 gtattgaagt ctatcgcgat agaccttcat cttcatggga atggcaaaat ggcaaagtta
     8581 aaatggatac tttagaagtt gatagccaaa ccttattaac acatcgtact gatgaaggtt
     8641 ggcaaggaat gccagcaaaa ggcatgattg gacacttaca tttaaaaaca catgatttag
     8701 acgcagcata tcaatgttac attgaacaat taggattcca acatgtgtct gattttccac
     8761 gtgcgctatt tatgtcaacg aatcattatc accatcatat tgctgctaat acttggcaat
     8821 ccaatcaagc aagacaaaac aattcacaaa gttatggatt aacacatgtt gatatttatc
     8881 aacccgacgc ctcaacacat acatttacgg caccagaagg gtttgacatc acagttcatt
     8941 caaatactga ccttgttcca gaaaaataat catccatact ttattaatac aattttttca
     9001 cgcaaaagcc tgcgattcat ttatgtaatc gcaggctttt ttattgctaa ctttttaatt
     9061 ttatatattt agacatacgg tattctattt attcttttgg tccaacccat tcaataacat
     9121 cttcataagc ttggcgtgtt ttatttttcg gtggctcttg ttgtctgtag ccaaatgcga
     9181 ccatcactga taaaccaaat tgctcagtat ctaagatccc tttatttgct aaaatgtctg
     9241 tcactgtatc cagactaaaa ccttccatcg gacatgaatc aatacctaac aatgcggctg
     9301 tcgtcatcat attgcctaat gcaatatacg tttgtttact tgaccagtca tacaaggctt
     9361 gatcattatc agaaatatgg aaatctgctt ggaatgcatc gaatttttgt tcaacagctg
     9421 gaatcgtttg tgcctcatat tttttaatat ctcttaacat atgttgtaca tacggtgatc
     9481 ttgacgttac atttttacgc gcaaaaatta gcacaaaatg gctcgctgta tctaattgtt
     9541 ttgctgcgcc ccaactgtgc gcttttaatt catcacgtaa cgcttgatct tgaatcacga
     9601 caaacttcca aggttctaac ccaagagaac ttggagacaa tctacctgac tctaatattg
     9661 tttcaaaatc ttctttcgaa actttctttt gtggatcgaa ttgcttagtc gcatgtctga
     9721 aatgaaatgc atccataatt gtttgattca tattgctcat tgaacatagc ctcccatttt
     9781 tatatttatt gctatacaaa ttatacttca tgttttaaaa agactaaagt aatccgtttg
     9841 caattcacta taacttgtgc acatgttata agttttctgc aataaaacac acttttgaat
     9901 tttgggaaat ttagtgttat aatatatttg tgatattttt cacaattaaa ttgtggcgtg
     9961 attttaatgt gcataatctt taatttcact catttacttt tcaatctttt aaaagcgagg
    10021 tttttaataa tgacaaaaat tatgttcttt ggtacgcgtg attatgagaa agagatggca
    10081 ttaaattggg ggaaaaagaa taatgtcgaa gtaactactt ctaaagagct attatcaagt
    10141 gctacagtcg atcaattaaa agattacgat ggcgtaacta caatgcaatt tggtaagtta
    10201 gaaaatgacg tttatcctaa attagaatct tacggtatta aacaaattgc acaacgtact
    10261 gctggatttg atatgtatga tttagattta gctaaaaaac acaatattgt gatatctaac
    10321 gttcctagtt attcacctga aacaattgca gagtattctg tttctatcgc cctacaatta
    10381 gtgcgtcgct tcccagatat tgaacgccgt gtacaagcac atgattttac ttggcaagca
    10441 gaaatcatgt ctaaaccagt taaaaatatg actgttgcaa ttattggtac gggtcgtatc
    10501 ggtgctgcta cagctaaaat atatgcagga tttggtgcta caattacagc ttatgacgcc
    10561 tatcctaata aagatttaga ctttttaact tataaagata gtgttaaaga agctattaaa
    10621 gatgccgata ttatttcttt acatgttcca gcgaacaaag aaagctatca tttattcgat
    10681 aaagcaatgt ttgatcatgt gaaaaaaggt gcaatcttag ttaacgcagc acgtggtgca
    10741 gtcatcaata cacctgattt aatcgctgca gtgaacgatg gtactttgtt aggtgctgcg
    10801 attgatactt atgaaaatga agcagcatac ttcacaaatg actggactaa taaagacatt
    10861 gacgataaaa cattattaga gttaatcgaa catgaaagaa ttttagtaac accacatatt
    10921 gctttcttct ctgatgaagc agtacaaaac cttgttgaag gtggtttaaa cgcagcatta
    10981 tctgtcatta acactggtac atgtgaaaca cgtttaaatt aatatatata tttaaaagca
    11041 aaggcgcgcc atgtgccttt gctttttatt ttgtatatct gtatcaaaat cgatttgatt
    11101 aaaatccgct ttattttatc atctattcaa atgattttag tgcgcttatt ttactatggc
    11161 attattgcct aacctgtttg agatatatta atatttgatg attcgtattc aaagttttca
    11221 tttaacatgt atttagtatc atgatagctg cttcattgat gatatactac ctactttttc
    11281 aacacttctt gttcgataat cgttaataca ccctgcttat cattacttgg agcgacagca
    11341 gaggcaatat tgaacaattc ttcatcatga ctattttcca tcacataact atgcttagcg
    11401 aacgctaaca tatctttatc attattcgca tctccgaagg ccatgagctc tgaaggagac
    11461 atttcccatt tatctaacaa tcgttttaat gcctgccctt tagtcatgtt tggcataata
    11521 atatcaatgc tatcgttccc acttgagaca agtttaatat cattgctgaa ttgcgttgct
    11581 acttcttcgt caacattcgg atgcgtctca cgattaatat taaatgctat tttgacataa
    11641 tcatcctcag gtaattgctg tagactgtca atttctttta actgatgata ataaaatctc
    11701 gtatcttctt taaacgcttc agaagtatgt tttaaaatat acgcgctttt caaaccacag
    11761 atgacgagtt gatcaatact ttgcttcata tttaagtaat cgacaacctg ttgaaacacc
    11821 tgacgattaa agcttttata attatataac tcattgccat tataaataac tgcaccattt
    11881 tcagaaataa aatacatatc tctatccccg aaaatagact tcaacttcgc atattgattg
    11941 ccactcgcag caataaatgt aatatctcta tttctaagtt cagtaaaaat cgcttcaaat
    12001 ctaagtttat catatgtctt ttttgaatct aaaaacgtcc cgtccatatc taccgcaata
    12061 gccttaacca tgattatcct cctcattgct cttttagtcg tcttcattat atccatctca
    12121 tgacaagcaa tgaagtttgt cataccacaa tgaacctata ttcacatttt gaaattgctt
    12181 tcatatatgc aaaacacatt acactggata tagcattcag aggcgaacat aaatttcaga
    12241 atattgtaaa aatatcacac tatatttatc atttagacaa aatatagtgt gatatactaa
    12301 tttataacat ctatacataa tgtgaaagaa tttttaaaaa tacaattaaa tatcggtgta
    12361 ataaatccct ttatacatag gagttgtata catgaaacta aataactatt ctttaaaagt
    12421 taaaaacaaa caactagttg acaattgtga tttaaatttc tatcttggtc agatcaatca
    12481 cattgttggt aaaaatggtg taggaaaatc tttattagct aaagatttct tactaaataa
    12541 tagtggaaat atccctaagt ccatttctca aaatgtaacc ttaatatcaa gttcatcaaa
    12601 tattcctaat gatataacaa aagatttttt attatcattg ttaaaatcaa aatttgaaaa
    12661 caatcgacaa acattcgata agatttataa catactaaac atcgaagcaa taccgtctaa
    12721 cgtactacta aaaaacttga gtgatggtca aaaacaaaag cttaaattat taagtttctt
    12781 attggaagat catgatttaa tcatattaga tgaagttaca aacgctttag acaagaaaac
    12841 agttaatgaa atttatgaat ttttaaatga ttttattcaa agccatcaaa ctaaaactat
    12901 tatcaatatt acacataatt tatccgattt aagtgctttg ccgggtaaat actttatttt
    12961 taaagacctt caaatagaag agtaccaatc aaaagaagaa gtcataaatg attacattaa
    13021 tttataattg aggtgtccac tttgaaatta gaatttaaaa aaagtatttc caataaaatt
    13081 atttacacgc ttgttgtgct attcacattc cttttcttac ttgggtattt tttaccaata
    13141 ggtatagaca aagttaaaag tttaagctat agccagtttt tcttcagttc atatactgtt
    13201 gcaacgcaat tagggttctt actattttca tttgttattg catattttat taataaagag
    13261 tattcgaata aaaatatcct gttttataaa ttgattggcg ataatatctt taccttcttc
    13321 tataaaaagg tagcagtgtt att
//
LOCUS       DANJFZ010000071        13301 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.071, whole genome shotgun sequence.
ACCESSION   DANJFZ010000071 DANJFZ010000000
VERSION     DANJFZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.071"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(224..1582)
                     /locus_tag="PWN45_001785"
     CDS             complement(224..1582)
                     /locus_tag="PWN45_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDL0623552.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1699..3252)
                     /gene="gntK"
                     /locus_tag="PWN45_001786"
     CDS             complement(1699..3252)
                     /gene="gntK"
                     /locus_tag="PWN45_001786"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL0623553.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(3276..3956)
                     /locus_tag="PWN45_001787"
     CDS             complement(3276..3956)
                     /locus_tag="PWN45_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0623554.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4110..4874)
                     /locus_tag="PWN45_001788"
     CDS             complement(4110..4874)
                     /locus_tag="PWN45_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0623555.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5039..5731)
                     /locus_tag="PWN45_001789"
     CDS             complement(5039..5731)
                     /locus_tag="PWN45_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL0623556.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5962..6378)
                     /locus_tag="PWN45_001790"
     CDS             complement(5962..6378)
                     /locus_tag="PWN45_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL0623557.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6517..8340)
                     /locus_tag="PWN45_001791"
     CDS             complement(6517..8340)
                     /locus_tag="PWN45_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0623558.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(8818..10095)
                     /locus_tag="PWN45_001792"
     CDS             complement(8818..10095)
                     /locus_tag="PWN45_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623559.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            10642..11253
                     /locus_tag="PWN45_001793"
     CDS             10642..11253
                     /locus_tag="PWN45_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL0623560.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(11262..11360)
                     /locus_tag="PWN45_001794"
     CDS             complement(11262..11360)
                     /locus_tag="PWN45_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623561.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(11350..12513)
                     /locus_tag="PWN45_001795"
     CDS             complement(11350..12513)
                     /locus_tag="PWN45_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623562.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            complement(12506..12964)
                     /locus_tag="PWN45_001796"
     CDS             complement(12506..12964)
                     /locus_tag="PWN45_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0623563.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
BASE COUNT         4277 a         2531 c         1861 g         4632 t
ORIGIN      
        1 atcattatct aatttaaaac aatatactaa acgtttcata attatcgcct gtacaatacg
       61 cacaaaaaca tgtcttgaaa cgcctttcat cactctaaaa tacccaatat actttttata
      121 tcgttcggat tctgagtatt tcagacgatt ttctgcataa aaataaacgt gtttcaaggc
      181 aatatattgc aattacctaa aaacacgttt acttaatatt tagttaaaca aataagctaa
      241 tgaataaaat gaagatgata cctgaaacgg aaataatcgt ttctaataat gaccatgtta
      301 agaatgtttc ttttacagtt aaaccaaaat attctttaaa catccaaaat cctgcgtcat
      361 ttacatgaga caaaatcaca ctacctgcac ctatcgcaag tacaactaat gcaacattta
      421 catctgatga ttgtaataat ggtaagacaa tacctgtagt tgaaatcgca gctactgtag
      481 ccgaacctaa tgcgatacgt agcacagctg caacaatcca tgctagtaaa atcggagaca
      541 tctctgtacc ttcaaacatt ttagcaattg tatttccgac accgccgtca attaatactt
      601 gtttaaatgt accgccaccg ccaataatca ataacatcat tccgattgga taaatcgcat
      661 tcgtcactga ttccataata tgattcatct tacgctttct cattaatccc atcgtaacga
      721 ttgcaaataa tactgctatt agcatggctg tccctgctgt tcctatcata taaatgatag
      781 attcaaatag atttgtaggt ttgtcatgcc cagttacaag ttgcgttatc gtagacacta
      841 acattaatat gactggtaat gttgctgtta ataaactcat accaaatcct ggcatctctt
      901 gatccgtaaa ttctttttgt gcacctaacg ctgaaatatc gccttctcgt gtatacgcag
      961 acggaatcat tttttgtgca actttgttaa atataggccc tgcaatgagt gtaactggaa
     1021 tggcaataat cataccatac agtaatacat ctccaacatt tgcctttaat tcttttgcga
     1081 tgactaccgg tcctggatgt ggtggtaaaa agccatgtgt cactgataaa gctgttacca
     1141 taggtagtcc tagttttaac actgaaacat ttgcgcgttt tgctactgta aatactaatg
     1201 gaatcagtaa gactaaacct acttcaaaga acaatgcaat accgacgata aatgctgcaa
     1261 caagcattgc ccattgtaca tgtttttgac caaatttttg aatcaacgtg tctgcgattc
     1321 gagttgcacc accaccatca gcaagcaatt tcccaagtat ggcacctaaa ccgaatatca
     1381 gtgcaatgtg gccgagcgta ctgcccattc ctttctcaat cgtctccata attttagtca
     1441 atggtatacc tagcattaac gctgtaatca tcgatgtgat aattaatgaa ataaatgtat
     1501 ttaatttaaa cccaataatt aatactaata aaataacgat acctaaaaca acactgatta
     1561 acggccatat ttcgttaaac atgacattcc cctctttctc ttttcaatag aatgtaacac
     1621 cgtcgtcgcg cacgtattcc acataatata tagtagtcat ggcacaacaa ctttatctaa
     1681 gatgcttgag tgacgtattt attgtgtttt attttcagcg atatgttggc gttgaaaatc
     1741 tgcaatttgt tcataattct ctgttaaaga acgacttaaa ttgataaaaa tggatacgat
     1801 ctcttggtaa acagtgacat tttcttcaat cggcgtatga ttgtttgtgg caccgaccat
     1861 cgatgaaacg attgaaaaat cttcaatgtc acctacagct ttaagtccga gcacgcaggc
     1921 acctaagcat gaactttcat aactttcagg aaccactaac tctgtgtcaa atatatctga
     1981 catcatttga cgccatactt cacttttcgc aaaaccacct gttgctttta tcatcttagg
     2041 tgtttcattc attacttcaa taagcgcaag atagacggta tacaaattgt aaagaacacc
     2101 ttctaatgca gcgcgaatca tatgttcttt tttatgagat aaagttaaac cgaagaatga
     2161 acctcttgca tttgcgttcc aaagcggcgc acgttctcct gctaaatagg gatggaatat
     2221 taaaccatct gcacctggtt taacacgctt tgcaatttga gttaagacat cataaggatc
     2281 aacaccgaga cgtttcgcag tttcgacttc actcgctagc aactcgtcgc gcaaccatct
     2341 caatacgaca ccaccattat ttacaggacc tccgatgacg tagtggtcct ctgttaagac
     2401 ataacaaaat attctacctt tgtaatcagt acgcggttta tctatcacag tacgaatcgc
     2461 cccagatgta ccgattgtga cagcaacttc tcctttacca acactattga cacctaaatt
     2521 agaaaggacc ccatcactcg caccaataac aaacggtgta tctttattaa gccccattaa
     2581 tgttgcataa cgttctttca tacctttcat cacatacgtt gttggaacta attccggcaa
     2641 catttccttg gaaataccca gcagttctaa tgcctcaaca tcccaatcta atgtttctaa
     2701 attaaacatc cctgttgcgg aagccattga ataatcaatg atatatgtat caaataaatg
     2761 atagaaaatg tatgttttaa tatctgcaaa cttagcagta cgttgaaata catcttgcca
     2821 ttcatgtttc atccaaaaaa tcttcgctaa tggcgacata ggatgaatcg gtgtgcctgt
     2881 tcgctggtaa atcgcattgc catcatgcac ttcatttatt actgttgcat attttgcagc
     2941 gcggttatct gcccaagtaa tattatttgt taatctttga tgttgctgat ccatcgcaat
     3001 caagctatgc atttgcgcac taaatgacac aaacttaatg tcgtctttat taactttgga
     3061 ttctctcata acatatttaa tagtcattag tactgcatca aataattcat ctgggttttc
     3121 ttctgagaca tcaacgtttg gtgtgtgtaa atcatagcct atttgatgtt tcatgataaa
     3181 agttccattt tcatcatata agactgactt ggtactcgtc gttccaatgt cgacaccaat
     3241 catatatttc atgataaatc cttctttctt tcattttaat tcaaccaaaa tccttcaata
     3301 tctttaccaa catcgtcgaa atttaaatga aacgcttctt tcaaaatttg actgtcgtat
     3361 tgttccactg catcaataaa cacttgatga ttatgatgta tgcgttcaaa atcttgcggg
     3421 ttctgttgca tacgttgtcg catggatgtt aaaacgagtg ccatcattac tggttttaaa
     3481 tgactccaaa atgagtttaa atattgatga ttagatgctt tgattaatgt ttcatgaaat
     3541 tcaaagtcat gcttcgtaaa tgattctgca tcctcaaatt ttactgccac tttcatcatt
     3601 tcaagttgtt tcttcatttc ttttacgatg ggtagtcgct cttgattttt aactcttgaa
     3661 aatgcaaatg actctaacat cagtcgcaaa tcatacattt ctttcttttc ttgttcccca
     3721 aacggcaaca catgtgcacc cattctttct aattggatga gttgattttg ttgcaataat
     3781 ttaaatgcat ctcgaattgg cgaacgactc acattaaatt gctttgccat ttgattttca
     3841 gtgagtaacg taccttcagc tatgtgacca ttcacaatgc ctaagcgtaa ttctgccgcg
     3901 ataccttctc cagttgtcat accttccaac catttctctg gatatccata catcatcaaa
     3961 gtcactcctt cattacacga catacttgta tacaagtatg ttaatatagt tattatgagt
     4021 ttgcaagcgc tttctttacg agcactaaaa tagtgaccac cccttttcga tttaaattta
     4081 aaggaaatgg tcactatcac acgaatgatt taattgttat gttgtatgtg ggatatttct
     4141 aattgttctg tactcatatg cgctttaggt acttcaatgc aataatgcgt ttcatgacag
     4201 tttggacatt cgaatcgacg tgttgtcgct gtatgtttcg ctttgataac tgcccacaaa
     4261 gatggtgaga atatatgctg gcagttagga cataaatagg caaccttttg ttggtaataa
     4321 aaagtaacac caatgccata accaatcata aatggtaaag caattaaaaa cggccattta
     4381 tttttcatca aaattgcact tataatgcta gaatattgaa ttattcctat aataccagca
     4441 ctaatccaaa tgttacgacg aatacttttc atttcagctg atttactcat gacatgctct
     4501 atgtctttta agtgtgtgat tggagacgtc gacgcttcat ttacgtaata ttgaacattt
     4561 ttaattttgt ttaataccgc ttgttgctgt ttaacttgtt ggttaatttc ttgttgtttc
     4621 atagttagta aagtattgag cgtcttcaaa gtaccttcac cttttagcaa catatctata
     4681 tcgcttaacg cacaacctaa atctttaagc aataagatta actctaatgt ttgtcgctgt
     4741 tgttctgtat acacacgacg ctttccttct gtaaatcctt gtggtttcaa aatacctttg
     4801 cgatcataat attgaatcgt tcgtgttgtc acattgcata attttgcgag ttctccagtc
     4861 gaatagttag acatagattc cacctcctat aattaccata gttgatgacc cgacgtcacg
     4921 agcaagtaca atttccacat tttaaagaaa tttattatac taggcgtctt atttttatga
     4981 tttcgtacca tgttgattta caaactcact caaactaagt aacacaccta ctaaacatct
     5041 actctgttat ttcagaatga atttgttgta atttatcttc aacttcagta atctctgtcg
     5101 cacattcttt cagtaaatct cgatactttt ccgtctctgc attgttttta taacgtattt
     5161 tatgttctaa acttgcccac atatccatac ctatcgttct aatttgaatt tcaacaggca
     5221 atacctcaac gcgttctgct aaaaagactg gaatagatac aacgatatgt aaactgcggt
     5281 aaccattttc tttagggtgc tgaatataat ctttacgttt tatcaattgt acgtcttctt
     5341 gtttaagcaa catctcttca atcaaataaa tatcatctaa gtaattacat actacgcgaa
     5401 ttccggcaat atccagtata tgttctttgg cagtttctgc gctaatctgt aatcctttac
     5461 gattaagctt ttctattaaa ctacgcattt ctttcacacg tcgctccata tgatgtatag
     5521 gattgtgttt ataaatgtga ttgaaattgt catctaaaat actcagttta gtgcttattt
     5581 cctttagtgc tgacgaataa atatgatcta actctacgaa acctaataac gtatcaaatg
     5641 cttcatcacc attttcaaat ttacttaagc tatttttaaa atcatgtcgc aaatcctcta
     5701 aatatagtga tggttttcga tctacataca tttttatact aacctccgat atataatcat
     5761 ctttattgta cctaacattt tattaagata atactaatac actatcgaac tttgggctga
     5821 tactgaaaca gcacaaagaa actcaaacaa ttcgaattat gtatcaaaac cttcaattac
     5881 aggaagtgat tttaaaatga tttaacgcaa aaaaacacct gttaccgtta tataggtaca
     5941 agtgcttaat tgatagagat gttatacgtc tttaaatgat tccacaactt ttggatgtgg
     6001 accatccata agcggttctc tttgtcgaac gccaccactt tggttgccaa ttgattcgct
     6061 atcaaagtac gatttatctt gatttgattg ttcttgaata tgttcttcat tatcagttgt
     6121 tgcatattca ctatccacct ctgttttttc cattgttgct gtatggaatt gtacgaagtt
     6181 ctcttcttct aatgctttga tttcttcttt cgataacgct cgataccaat ctttcgcttt
     6241 tttcgccgca ataggaacaa catctttttc aagatatcct tcttttaaca ttgcattcgc
     6301 aatttctatc gctttttgcc gtttaattac ttcaaattct ttccattttt cggggtaacc
     6361 tttcattgta aagggcattt ccttaacctc caatatgtta taattcatgt tatataccac
     6421 cataattata aaatgaaaca tattaaacac atttttttag actcccgtca taataacttc
     6481 agacaaacgt tagtctgacc tgataaatat ttaattttaa acgttcttgt ctctctcacg
     6541 aaaaccattg tgttgaatat tcatcattgc tgattgaata taattatact gtggtaaatc
     6601 tggtaatatc tttatttcta attctgtatt taattcgaaa tgcttagcga tgttttcaaa
     6661 tattgctaaa tactcgccca ttaattgttc atttattgta agtctatctt cattagccat
     6721 ggctctattt aacataaagc taatttcttc taatgcgaat aagctaggat aataattttg
     6781 aatcaatgtc ttatcactaa ataattcacc attagctgca ttataaactt gcgtcatgtt
     6841 atttaatttc acagataggt tcatagccgt gtttttctga tacacatcat ctttatattg
     6901 attttcagaa aataaataat gaaagagcgt tgcttcttta cgaacaactt cagccattac
     6961 attaggaagc aatgcggatg cggtacgttg tccaaggaca aataaaccta tgattgcaat
     7021 cacaataccc attgcaacgt caataactct tggaaacgca atgttaattg ttaaattctg
     7081 tgatgctaat ccgttcatta aaataacttg tattgtaata aaaactactg caatcgcata
     7141 atttgctccc accaatgctt cagtaaacaa tgctgcaatg cccattaaaa ttactgcaac
     7201 aggtgttggt attgaaaaca acaatatgac tgaaagtact aacacaccta aaatagtacc
     7261 taaacctcgt gccatacctc tctcgattgc atgtatagtt gatgttccta gtaatattgt
     7321 atgtgcagat aacggtatcc agtatgcttt ttcaaaatca aacattaacg caataaatat
     7381 cgctatcatc ataatcgctg tatatctcaa tgtatttcta aaaacaatag agtctaatgt
     7441 taaattttga taaatgcgtt tactatataa aggttttcgc atgtctgctt catacgcaat
     7501 acgcgcttca tttgcatgca ccatttcatc aattttcaat atatggttga ataaattttg
     7561 aaattcctct gttactgtca cttcttttcg ccaatatctt acattttcct cactattatc
     7621 tagttgtgca atgatatgat ccatcatttc aattaactca tctggcaatg gtcgaatttg
     7681 tttagcgttc aactctaaca gttcagaata aatcccttgg gcagatgtgt gtaataataa
     7741 taatttttgg aaacgtctac ttaatttatc gttacttgaa gtagaagtga ttaataattt
     7801 atcagatgct ttaaaactat ccaccgctgt ttttgctact tgtaaaaaag cagatttatc
     7861 attataagtg tgtaacaact tagatatgag tttaaaatca ttttgaattg cttgttcttc
     7921 agctttgttt ttagaaaata cgatcgtgat taacaccatc atagtggcaa gtataccgcc
     7981 tactaaaatc gcaaagcctc tatataatgc ttcttcggga gctataggta agttaatggg
     8041 tagactgaat gtcacaatga agaatgtcga tgacggtcca gctattttta aggcagtaaa
     8101 tatataaaat ggtataaccg taacaattaa taatgtcatt ccaaaaacga gtggcgtttt
     8161 ggctgttagc gtaccaagca tcatacatat tgcaaacgct aaattacaaa ttattacagt
     8221 tcgcagctta gatcgcgacg gacctttaaa aacataaata tgggctagtg ttccggttgc
     8281 aactaataat ccaaattgga aattaccaca taagtaaccg attattgcag gaataatcat
     8341 taacagtccc tgtcttacac ctttataaaa gtctaagtta agtcgattga tttttatgag
     8401 tgatgtcaaa aagatgtcat ttcgacttct cctttccttg tcaaaatatt tccataatct
     8461 ttttattagc atagttaaaa tgaatttgtt gctataaatt ttaattacgc aacatatgtc
     8521 attataacac cttatgctca attatctaaa tttaaaaact tttaaagtcc aattaaaatt
     8581 aatttctaat gttatttaac atcgttgaac ctttggtcgc ccacctttgc gctagttaaa
     8641 atttatatgt acttttacaa gcgttacctg actaatgttt taaaacctta tttctttaca
     8701 tatatcattt caataacttc attttcatct taaaccatat ctgttttaaa aagtttcaat
     8761 ttcaacgcaa aaaaagcaat aagccccaac tatgtgaaag cttactgctt gtttaaatta
     8821 tttttctatt gctttatcat cactaacaat taaaggttga tctggtttca catagaacca
     8881 cataatcaca gcgattactg ctggaacgat taacaattgg aatgtcattg tccaacccaa
     8941 atgttctacg aatagacctg ctaatagcgg actgattaat gcacctatat taccccataa
     9001 gttcatccac cctgatactg taccagagaa gttacgtcct aagtcagttg ctgcagccca
     9061 actcataccc attgaaatac cgataccacc aagacctagt gataaccaga aaatacttac
     9121 atataaattt cctgtgcgta cagcaaagat aattgaaact gcaaacacaa taaatcctgc
     9181 aattgcaatt acacctctag ctacgaattt tgaacgacct aatcctaata cacggtcaga
     9241 aattgcacct gctgataaga ttaagaagaa cattaataac caaggtaatg aactaatgct
     9301 catttcttta aagttaacgt ggaatacttc tgttaaatac gtcggtaacc aaattaagaa
     9361 taatgtaatg ataaattgta caacaaagta ttgaattgca attgcataga agctaaaacg
     9421 tttaaagaaa tcattccatg gtggtgatga cttttcagta gctacgatat cacgattttc
     9481 cataatgaaa cgtttctccg cttcattaac cattctatgt tgctcaggta agtctttggc
     9541 aataatcgcc cataatacag ccattaaaat acctactgca ccaaaaatgt aaaatactgc
     9601 ttgccagtta aatgcgttaa caatagcaat tgtaactatt ggtgctaata caggtccgaa
     9661 atatgatcct gctaataatg cacttgatgc tctacctttt tcatttttag agaaccaaaa
     9721 tgaattaaac acagcattag aagggtacat tggcgcctca cctacaccaa ataagaatct
     9781 cactaaataa attaaaccgt ggttcttaat catacccgtt aagattgtaa atgcactcca
     9841 ccaaactaat gcaattgtaa tcatcttacg tggaccgaat ttttcagcca acatccccga
     9901 tggtacttgc attaatgcat aacctagaga gaagaatgat gccaataaac caaattgtgg
     9961 ttttgtcatc cctaaatcat ccatcatttg cttagcgata tatgaaatgt ttgctctatc
    10021 catatacgca ataaccccaa taaagaagaa cgcaagcgcg aacatccacc ttacgttagt
    10081 tcgtttttcc ttcataacta ttcctacttc cctaattaat tttttaaaac ttaccctgaa
    10141 acgttaaaat tttcggacat ctggcatgtc cacccctact ttgtcacaaa attgtaccaa
    10201 agttattttt tataatatta tcagacatgg aaagcgttta caatagaatc tataaaaatt
    10261 ttattaaatt ccgcttaact tttgctatta aaacgatata aaggtacttt tagttacata
    10321 gtagcctatc gtttgttaat taaaaatact gcaatcctag tgttttaaaa cgtatattgc
    10381 aggctttctt acatattcga cattcatttt aaattgcata taactacatt aaccattcat
    10441 tgtgcagcgc ctgttgttta gatgatcatt tattgacttt ttgctcttat agcaagcaat
    10501 tttttgattt attgcttata atacatttta agacaaatgt ttgtttcaac atcgcatgcg
    10561 acatcattaa ttattcattt gaatttatcc agtttctatt tgtctgcaat tgctgaaaat
    10621 taaaggagct tatttttaat aatggaacaa attatcactg aatttattag ccgttttggg
    10681 tatgcagcca tttttatatt aattttatta gaaaatgtac tacctatcgt accatcggaa
    10741 attattctga catttgctgg tcttatgtct gttaaatcac atttatcaat tttaacttta
    10801 tttattattg caactatcgc atcgtttata gggctgttaa ttttatatta tatctgccgt
    10861 ttgatttcag aagaacgtct atatcgtttt attgatcgac acggtaagtg gattaaattg
    10921 aaaagtaagg atttaaagcg agcaaatgat tggtttaaaa agtatggcgt atgggctgta
    10981 tttatctgtc gtttcatacc tgtattacgt gtattgatta ccattccagc tggtgtaaac
    11041 cgcatgaatg ttgtgacatt taccgttatt tcattaatag gtactacaat ctggaatttc
    11101 ggtttaattt tactaggacg cactttgagc gatagctttg gtatgttgat gactggtctt
    11161 catacatatt cacgtattat gtatgttgtc atcattatcg cagtcattta ctttgctata
    11221 cgttacattg gcaaacgtaa gagagtaaaa taatattgtt gttagcataa aggcatgaat
    11281 tttaaatttc tataaacaaa aagcagtgaa tcattccatc tattgaaatg attcactgct
    11341 tttttaagtt tatttggcat actttagctg aaagtcaact atctttatat aaagaaatat
    11401 tactattaac atgatagtta tagttgtgaa gtaaaaggct accaacggct gagaaatgat
    11461 accaattaat aatgcaccga cagtcattcc aatatatctc acaaaattaa atacacctag
    11521 cactttccct tttatatgtt caaacctttt agaaattata gtagacagca aagctggcac
    11581 aataccaaca caagtaccta atataaatat aattactgat aatataatta catttgattc
    11641 gtttaaccat ccaaacaata acgtaaatat agcaaatcca gttatagtaa cacgaagtat
    11701 cattatttca ttatattttt ttgaaaatcg cttataaaat aaacttccta atataacact
    11761 aacagtaata ggtaaaaaca aaactcctga tattccaacg cctatatcat aggtattatt
    11821 taataatgtt ggtaaataaa ctaatattga aaagtacgta aaaaatatta aaaatcccaa
    11881 tgtcactaaa gtaataaaca ctttatcggt caaatatttt tcttcaattt tttcagttaa
    11941 ttttacaatc cctttttcaa catttggaat atcaataaaa aagataatca aaaataatac
    12001 gatagatata acgagcaaaa aactaaaaat accaatataa tcccatcttg cacctaaagt
    12061 actccctaag attggtgcca aagctggtgc acatgataaa aatatttgat agttcgccat
    12121 agcttgagct ctaccattat ctgtacttag tttggctaat aaagttaaag tcacgagagg
    12181 aataattccg caaccaattg cttgaaataa ccttgatata gcaaataata taaagttatg
    12241 tgtcactgca caaataaagc tagatatgat tactattccc aatcccgtta aaagtacttt
    12301 tttcgaatca cgagaatcta tacttctacc aagtacaatt tgcataactg caacgataaa
    12361 tataaaaccg ccaactgtcg catttatcca aaatagtggt acttgaaaat cgtcatatag
    12421 tctcggaata ataggcgtgt atatgttttg aatcaatgat gcgaaaaatg ctaaaaatcc
    12481 tagaatatat aatctgatat tactattaat catttaaacc atcttctttt tttaattctt
    12541 ctatactacg gtttaaaaac tctataataa ctgccatttc atcatcatca aagactttaa
    12601 gtacctcttc atattttttt accgattttt catgtaattc atcatgtata taagataatt
    12661 tttttccttt ttgtgttaat gaataattaa cttctctttt gttaaattca ttgtgcgatt
    12721 caactactaa gtctttcgct aaaagttttt ttatcgattt agtaattgct ggttttgata
    12781 catttaacat ttcagacaaa aatttattat tcactttatc attattatca atcaactcta
    12841 tgatatgaaa ttgtgttaga gataaatcta gttcctcatt ttccttttta tactgtcttc
    12901 ttttatcggc attttctctt tctataataa attgttgtaa ttttaaaata acttctctct
    12961 tcatatccac tattattcct cctcttcatt agttaaccgg ttaactatat tattgttgtc
    13021 actcccattt ttgtcaaata ttgttgtaat taacatatat ttacttagtt atgcaccatt
    13081 tattccgcat ttcacaaatc cacctataat acaaactatc attttaaata agcagaattt
    13141 accctgtctt ccacacaaat tgttttcgac actttaatgt gcctactatc caaaaaagtt
    13201 actcataaca aattgcgcat tttaataaat gtagcaatgc catttgcatg ctgcaaaaat
    13261 actcgtattc atatttatgt tttatattat taaagttttt t
//
LOCUS       DANJFZ010000072        13197 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.072, whole genome shotgun sequence.
ACCESSION   DANJFZ010000072 DANJFZ010000000
VERSION     DANJFZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.072"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            59..1150
                     /locus_tag="PWN45_001797"
     CDS             59..1150
                     /locus_tag="PWN45_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0623564.1"
                     /translation="MIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGY
                     PYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFP
                     KMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDI
                     LNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDN
                     AVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQG
                     LSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAA
                     EFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1239..1595)
                     /locus_tag="PWN45_001798"
     CDS             complement(1239..1595)
                     /locus_tag="PWN45_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL0623565.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2072..3031
                     /locus_tag="PWN45_001799"
     CDS             2072..3031
                     /locus_tag="PWN45_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0623566.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3077..3208)
                     /locus_tag="PWN45_001800"
     CDS             complement(3077..3208)
                     /locus_tag="PWN45_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623567.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3272..3487)
                     /locus_tag="PWN45_001801"
     CDS             complement(3272..3487)
                     /locus_tag="PWN45_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDL0623568.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFCTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            3652..4203
                     /locus_tag="PWN45_001802"
     CDS             3652..4203
                     /locus_tag="PWN45_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623569.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4255..5193)
                     /locus_tag="PWN45_001803"
     CDS             complement(4255..5193)
                     /locus_tag="PWN45_001803"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0623570.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            5375..6736
                     /locus_tag="PWN45_001804"
     CDS             5375..6736
                     /locus_tag="PWN45_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDL0623571.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            6723..8396
                     /gene="menD"
                     /locus_tag="PWN45_001805"
     CDS             6723..8396
                     /gene="menD"
                     /locus_tag="PWN45_001805"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL0623572.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            8383..9186
                     /gene="menH"
                     /locus_tag="PWN45_001806"
     CDS             8383..9186
                     /gene="menH"
                     /locus_tag="PWN45_001806"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL0623573.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9179..10000
                     /gene="menB"
                     /locus_tag="PWN45_001807"
     CDS             9179..10000
                     /gene="menB"
                     /locus_tag="PWN45_001807"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL0623574.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(10239..10568)
                     /gene="sspC"
                     /locus_tag="PWN45_001808"
     CDS             complement(10239..10568)
                     /gene="sspC"
                     /locus_tag="PWN45_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDL0623575.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(10606..11787)
                     /gene="sspB"
                     /locus_tag="PWN45_001809"
     CDS             complement(10606..11787)
                     /gene="sspB"
                     /locus_tag="PWN45_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDL0623576.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(11869..12879)
                     /gene="sspA"
                     /locus_tag="PWN45_001810"
     CDS             complement(11869..12879)
                     /gene="sspA"
                     /locus_tag="PWN45_001810"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDL0623577.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
BASE COUNT         4698 a         1832 c         2376 g         4291 t
ORIGIN      
        1 ttttgatata acgactggta catatattgc ctacattaga aaaagtaaat ctgaaaaagt
       61 gattgatttc tttaaagata ataaacgaat tgaacggttt agttttatag ataataaagt
      121 gcatatgaag gaaacattta atgtagataa taaagtttgt tatcaagtat tttatgatga
      181 aaagggatac ccatatattt caaggaatat taatgctaat aatggtgctg taggtaaaac
      241 ttatgtgtta gttaataaaa aagaatttaa aaacaattta gcactgtgtg tttactattt
      301 agaaaaacta ataaaagatt ctaaagatag tattatgatt tgtgatggac cagggagttt
      361 tccaaaaatg tttaatacaa atcataaaaa tgctcagaaa tatggcgtta ttcatgttaa
      421 tcatcatgaa aatttcgatg atacgggtgc atttaaaaaa agtgagaaat atattattga
      481 gaatgcgaat aaaattaacg gtgtaattgt attaacagag gcacaaagat tagatattct
      541 taatcaattt gatgtagaaa atattttcac tattagcaat tttgttaaga tacataatgc
      601 tccaaaacat tttcaaactg aaaaaatcgt aggtcatatt tctagaatgg taccaacgaa
      661 gcgaattgat ttgcttattg aagtggctga gttagtcgta aaaaaagata atgctgttaa
      721 atttcatata tatggagaag gatctgtcaa agataaaata gctaaaatga ttgaagataa
      781 aaatttagaa agaaatgttt ttcttaaagg atatacaaca actccacaaa aatgcttgga
      841 agattttaaa ttagtcgttt ctacatctca atatgaaggt caagggttaa gtatgataga
      901 agcaatgatt tctaaaaggc ctgttgttgc ctttgacatc aaatacggac caagtgattt
      961 tatagaagat aataaaaatg gttatttaat agaaaaccat aatattaacg acatggctga
     1021 taaaatactt cagcttgtta ataatgatgt attagcagcg gagtttggtt cgaaagcgag
     1081 agaaaacatt atagaaaaat attcaacgga atcaatatta gaaaaatggt taaatctttt
     1141 caatagctaa atgaaaaaat cctaggtatt catgagtacc taggattttt acgcatgata
     1201 aatacaaaaa aagagaggat agtcctctca aacaatagtt aataaaattg tagccatttc
     1261 ttccagctgt aactactaat acaagctaag ttcattacga tatgtatgat ttcaaattca
     1321 taaccactta catatcctct tgtaagttta agtgtgaaga ttgagcctaa cataacacaa
     1381 atataaaaaa cattaacata aggcgttaaa aagccgataa taagtgctaa acctccaaca
     1441 acttcaccaa ttgacatcaa tactgcgatg gtaggtggta aacctaaaga gcctaagaag
     1501 tgaattgttc catctaaatc aacaaatttc aaagtaccat gcactaagaa tgatgttgct
     1561 aacatccatc taattaaaag catacctaat ttcatcatgt cacctccaac attcatattt
     1621 ttaaaacata gtccaaatta tatttgtaaa catgtggaaa gtaaagattt tttataagat
     1681 taattattaa atattctatt gtattgaaca attgttgtag aaagatgttt aagtcatcag
     1741 ttactttttg tatttataaa taaagattag tacagcatag tgcaaagatt atagggaaat
     1801 gaattaacta ttgcatcgct atttatgaat cgtaaacact attatgaaat tgcgagagta
     1861 gtatggacaa ttagaatgat ggtttgagat aaatgagtgt tattaaccag tgcgtcattt
     1921 tcaaaattag atgccatttg ctgtttataa taagaagatt tgataattaa ctatgataag
     1981 aaaaaataat aatcccctaa atacattata aacaaattaa atgataaaat aataatatat
     2041 atttctacta aagttgtttg gaggtaaatg tgtgaatagg aatatcgtta aactagttgt
     2101 gttcatgcta attttagttg tagcagtagc gggttgtggt caaaaagata ctgaagagaa
     2161 aactgaaatg acgacaataa aagatgaatt aggaactgaa aaaattaaga aaaatcctaa
     2221 acgtgttgtt gtattagaat atagttttgc tgattattta gcagcattag atatgaaacc
     2281 tgttggtatt gcagatgatg gcagcactaa aaatataaca aagtcagtaa gagataagat
     2341 tggggcatat gaatcggttg gatctagacc gcaaccgaat atggaagtga taagtaaatt
     2401 aaaaccggat ttgatcattg cagatgttag cagacataag aaaatcaaat cagaattgag
     2461 caaaattgct ccgacaatca tgttagttag cggtacggga gattataatg caaatattga
     2521 agcatttaaa acagtcgcta aagcagtagg caaagagaaa gaaggcgaga agcgtctgga
     2581 aaagcatgat aaaatattag cggagattag aaagaaaatt gaacagagta cgttaaaatc
     2641 tgcatttgca ttcggtatct caagagcagg tatgtttatt aataatgaag atacatttat
     2701 gggacaattc ttaattaaaa tgggtattca acctgaagtc acaaaagaca aaactacgca
     2761 tgttggtgaa cgcaagggtg gtccttatat atatttaaat aatgaagaac ttgccaatat
     2821 caatccaaaa gttatgattt tagccactga cggaaaaacg gacaaaaata gaacgaaatt
     2881 cattgatcct gcagtttgga aatcattaaa agctgtgaaa gataacaaag tttatgacgt
     2941 tgaccgaaat aagtggttga aatcaagggg gattatcgca agtgaaagta tggcagaaga
     3001 tttagaaaaa attgcagaaa aagcaaaata aaaatacagc gctactcgta aatcatataa
     3061 gagtggcgct gtatttttaa ttatgtttat tatctgtcgg atgtgatgat ttacctgaaa
     3121 gtttatttcg aataaattta attacataac cgacaaggat tgttttaaca gttcttttaa
     3181 tgaattggcg catcgttaca tacctcattt ctctatatct tacgaactat atacccattc
     3241 atatatgctt tttaaacgtc attgtcacaa tttaattttt agggaatata atataaccat
     3301 ctttatctgc ttttttagta aaaatgacaa aaattgcatg tattattgag atgatggtag
     3361 ggatacctgt gcagaaaaat aataagtgaa aaagaccttg tccaaattta tcagcataaa
     3421 atttatgaat acctaaacct ccaagaaata atgcaacaat aacataaatg gctttattga
     3481 ctttcatttg taaatcctcc ttaactataa ttctacttaa attcgttgtg aaaaccaata
     3541 tttctaactt tagaattttc aaactttcta aaattataag tatatctttt taaaataagc
     3601 tagaatttct atataataaa tgttaataac gtaaaaggga atgatgacat agtgatacgt
     3661 caagcacgtc cagaggaccg atttgatatt gcgaagttag tttatatggt ttgggatgat
     3721 atggaattag aattggtaaa gcatctacct aaagacatgg tattagatgc aattgaaaaa
     3781 agctgtgttg atgcaacata tcgaactttt tatcagcata ttttagttta tgaagtagaa
     3841 aataaagtag caggttgtat tattagctat agtggtgaaa atgaattgaa atacgaaaaa
     3901 gcatgggaac tacttgactt gccagaaaaa ataaaacaat atggcacgcc attacctgta
     3961 aaagaagcta aagacgatga gtattatata gaaacaattg cgacatttgc agcatataga
     4021 ggtagaggca tcgcgacaaa gttattaacg tcattacttg aatcaaatac acatgttaaa
     4081 tggagtttga attgcgatat taataatgaa gcagcattaa agttatataa aaaagtaggc
     4141 tttatatctg atggacagat tgaattatac aagcacatgt atcatcattt aattgttaaa
     4201 taaaaaaact cgacagttcg atgtaagtcg attgccgagt agtatcattt tctattaaat
     4261 gcctgcaaat aatgcactaa tataaatacc taatgcatat aataaaccga aaaatgtatt
     4321 tgttttacca gcagcagcca ttgctggcat cattgtaggc ggtgtatcat tcttcttgaa
     4381 acgtctgata actttaacag gcattgggaa tgataacaac gcaagtaagt aaaataatga
     4441 gccaccaggt ttaataatga tcgtaagtac aataaaggca taagcgataa agtacatgat
     4501 tgccataaat gttaaagaag catttttacc taatagaatg ggtaaagttt tgcgaccact
     4561 tgctttatct ttgacacggt cgcgaatatt gttagccata ttaattaaac cgatagtgat
     4621 tactataggt acacttaacc aaattacata actttgaata ttgccagttt gaataaagaa
     4681 tgcaataacg ataataaaca tacccataaa tacgcctgag aataattcac cgaaaggcgt
     4741 ccatgaaata gggaaagggc cacctgtata taggtaacca acagccatac atactaatcc
     4801 aactggtaat aaccaaaatg aagagttagc agctaaaaac aaacctaata ttgctgctaa
     4861 gatgtaaaat gcaatggcta atcgtagcac aagctctggg ctcataccgt tgcgaacaat
     4921 ggcaccacca atgcctacag attcatgatc atcaaggcct tttttataat catagtattc
     4981 attaaacata ttagttgctg cttgaataag taagcatgct agtaacatgg caatgaatag
     5041 gctgatttta atatgatctt cgctaccaag aaaatatatt ttagatgctg ctgtaccaac
     5101 taaaacgggt actacggaag cagttaatgt atgaggacgc attaaatgcc aatatttctt
     5161 aactgtagaa tattgctgat attgattact cattgaatac aaaacctctt taaatcatat
     5221 tttttataac aaagatattt taatagaaag tagcataaag gtcaattgac gtgaaaaatt
     5281 gcaagataag tttaagcaat tcttgtaaca caattatcca aaaatgatac aatataataa
     5341 tgagtgaaaa aagacgaaga aagaagtgaa atggatggct acgggcgtat tagaggacga
     5401 tattgtcaaa gagatatatg gcagctcaaa ggaatgggtt tcagttgaag tgaaattatc
     5461 acagtcacta gacccgagca cattatttca tctcactgac aatgaggcag gagatcgctt
     5521 ttatatgcgt ttgaatgata atcgaacgtc atattttggc tacaaagcaa ttcaattatt
     5581 caaaaataat tctaaaaata aacaatctat ttttaaagac tgggaaaaat taaaacataa
     5641 catcacattt atacatccgc aatctgagaa acatcatctt cgagttgttg gagggttcca
     5701 attttcaagt cataaatcag atgatgaatg gcgagagttt ggactaaatc attttgtatt
     5761 acctgaagtt ttaatttcaa ctgataataa tgggacattt ttaacttata cagttaaaag
     5821 ggaaagtttt actgttgagg cattgaacga tttaatggat ttgttcaaca atatatcgga
     5881 catagatgtg gacgagcaaa ttgggggaat tactagaaat gaagatattt ataaagatga
     5941 ctggcgtcaa cttgtagtag aagctataga atctattaat aatgaagaaa aaattgtact
     6001 agcacgtaga cggttaataa agttcgataa agatatcagt attccatata ttctaaagca
     6061 agcatattct aaagaaaaaa acagttatat attcttgtta gaatcacaag attctatatt
     6121 cttttcacaa acacctgaac aattaataaa ggtcaataat aaaatactat cgactaaagc
     6181 tgtagcaggt acaattaaac gttcacaaga tgaggacgaa gatacaaaaa atgttgaagc
     6241 atttttaaaa gataataaaa acttaatcga acatcgattt gttgttgaca gtattttaca
     6301 tgatattaaa ccttatatca ctgaattaca ttatgataag acgcctaaaa ttctaaaaaa
     6361 tgatcattta tatcacttgt acactgaaat aaaggcgcca ctgaaggatg attcgtatat
     6421 tagtttaatt gataatttac atccaacacc tgctttaggt ggctatccaa aagaatttgc
     6481 aatggatttt attgagcaga aagaatttgg tacgcgagga ttatatggtg caccggttgg
     6541 ctatatagat atatacgatg attgtgaatt tattgttgca attcgttcga tgcttattaa
     6601 gaaagcacaa gcaactttat ttgctgggtg tggcattgtt aaagattctg atccagatag
     6661 tgaattggca gaaacgaacc ttaagttcac acctatgatg aatgcattag gagtcgatat
     6721 gaatgggaaa tcataaagca gctttaacga agcaagtttt tacatttgca tctgagttat
     6781 atgcgtacgg cgtaagggaa gtagttatca gtccgggatc acgctcaacg ccacttgcac
     6841 ttgcatttga agcacatcca aatattaaaa catggataca ccccgatgag cgaagtgcag
     6901 cgttttttgc agttgggtta attaaaggca gtgaaagacc tgtcgctata ttatgtacgt
     6961 caggtacagc agcagcgaat tatacgcctg caattgctga aagccaaatt agtagaattc
     7021 ctttaatcgt tttaacaagt gaccgtccgc atgaattaag aagtgtaggc gcaccacaag
     7081 cgattaatca agtaaatatg tttaataatt atgtaagtta tgagttcgat atgcctattg
     7141 cggatgatag taaagagacc attgatgcaa tttactatca aatgcaaatt gctagtcaat
     7201 atttatatgg accacataaa gggccaattc attttaactt gccatttaga gatccgttaa
     7261 cacctgattt gaatgcaaca gaattgttaa cttctgagat gaagatttta ccgcactatc
     7321 aaaaaagtat agatgcatcg gcattaagac acattttaaa taagaaaaaa ggtttaatta
     7381 ttgtagggga tatgcagcac caagaagttg atcaaatact aacgtattca acgatatatg
     7441 atttgcctat tttagctgat cctttaagtc atttaagaaa atttgatcat ccgaatgtta
     7501 tctgtacata tgatttgctg tttagaagcg gcttagactt aaatgtggat ttcgtaattc
     7561 gtgttgggaa accagtgatt tctaaaaagt taaatcaatg gttaaagaaa actgatgcat
     7621 ttcaaatatt agtgcaaaac aatgataaga ttgatgtctt tccgatagcg ccagatattt
     7681 catatgagat ttctgcgaat gatttcttta ggtcattaat ggaagacacg acagttaatc
     7741 gcgtaagttg gttagaaaaa tggcaatgct tagagaaaaa agggcgtaaa gaaattaaat
     7801 gttatttgga acaagctaca gatgagagtg cattcgttgg tgaattgatt aagaaaacat
     7861 ctgaaaaaga tgcattattt attagtaata gtatgcctat cagagatgta gataacttgt
     7921 tattgaataa aaatatagat gtctatgcga atcgtggtgc gaatggtatt gatggtatcg
     7981 tttcaactgc actgggtatg gctgtgcata aacgaataac attattgata ggtgatttat
     8041 cattttatca tgatatgaat ggactattaa tgtcaaaatt aaataatatt cagatgaata
     8101 ttgtattatt gaacaacgat ggtggcggta ttttttcata tttaccacaa aaagaaagtg
     8161 caactgacta ttttgaacgg ttgtttggca caccgacggg attggatttc gagtatacag
     8221 ctaagttata tcaattcgat tttaaacgtt ttaacagtgt ttcagaattt aaaaatgcca
     8281 cattgttatc tgaaacttcg acgatttatg aattgataac gaatcgcgaa gataacttta
     8341 aacagcatca aattttatac cagaaattga gtgaaatgat tcatgacaca ttataaattt
     8401 tatgaagcaa acgttgagac caatcaagtt ttagtatttc tgcatggatt tcttagcgac
     8461 agccgtactt atcataatca catcgaaaaa tttactgata actatcatgt catcactata
     8521 gacttaccag gccatggcga agatcagtct tcaatggatg aaacgtggaa ttttgattat
     8581 attacgacgt tgttagaccg aattttagat aaatataaag ataaatcaat aacattgttt
     8641 ggatattcaa tgggtgggcg tgttgcatta tattatgcaa ttaatggtca catccctata
     8701 tctaatttga tattagaaag tacgtcacca ggtattaaag aagaagcaaa tcaattggaa
     8761 cgccgtcttg ttgatgatgc acgtgctaaa gtattagaca tagcaggtat tgaattattt
     8821 gttaatgatt gggaaaagtt gccattattt caatcgcaac tagaattacc agttgaaata
     8881 caacatcaaa taagacaaca acgattgtct caatcgccac ataaaatggc caaagcatta
     8941 agagattatg gtacaggtca aatgccaaac ttatggccgc gcctgaaaga aattaaagta
     9001 ccaacattaa tattagctgg agaatatgat gaaaaatttg tacagattgc gaaaaaaatg
     9061 gcaaatttaa ttcctaatag taaatgtaaa ttaatttctg ctacaggtca tacaattcat
     9121 gtggaagata gtgatgaatt tgatacaatg atattaggat ttttaaagga ggagcaaaat
     9181 gactaacaga caatgggaaa cacttagaga atatgatgaa atcaaatatg aattttacga
     9241 agggattgct aaggtaacaa taaatcgccc tgaagtacgc aatgcgttta cacctaaaac
     9301 agttgctgaa atgattgacg cattttcacg tgcacgtgat gatcaaaacg tttcagttat
     9361 cgtattaact ggtgaaggtg atttagcatt ctgttctggt ggtgaccaga agaaacgtgg
     9421 acatggtggt tatgtaggtg aagaccaaat ccctcgctta aatgtattag atttacagcg
     9481 tttaattcgt attattccaa aaccggttat cgcgatggta aaaggttatg ctgtaggtgg
     9541 cggtaatgta ctaaatgttg tttgtgactt aacgattgct gctgataatg ctatttttgg
     9601 acaaactggt cctaaagtag gttcatttga tgcgggttat ggttcaggat atttagcacg
     9661 tatcgttgga cataagaaag cacgtgaaat ttggtactta tgtcgtcaat acaatgcaca
     9721 agaagcttta gatatgggtc tagtaaatac agtggtacct ttagagaaag ttgaagatga
     9781 aactgtgcaa tggtgtaaag agattatgaa acactcacca acagcgttac gattccttaa
     9841 agcagctatg aatgctgaca cagatggttt agctggttta caacaaatgg ctggggatgc
     9901 aacattgctt tattacacaa ctgatgaagc gaaagaaggc cgtgatgcgt ttaaagaaaa
     9961 acgtgatcct gacttcgatc aattccctaa attcccataa gttattttga atatgatatt
    10021 aagtcacttg cgtcgtttat tagcgacgat aggtggcttt tttattttat aagaatttaa
    10081 aagggcattt taattaatta tcataaagat aagatagttt gagaaaaatt aatagagaaa
    10141 gaataaatgt taaagattat tattacaatg aaaaaacgag ctggtgagaa attgaattct
    10201 cgccaacccg ctttatgaag tgaaatcatt taaattagtt atactaagcg ctcataaacg
    10261 attggtcgcg aagtgccaat accttgtttg gatgacgttt gcatgaggat atgctgtgta
    10321 tcatcataat caagaatata aatgatttct tcattatcaa tagatgaaaa tttaatgcgt
    10381 tgatgtgccg tatctataaa aagaatatga tattgattgt gacttgtatc atcgccatga
    10441 cgtatacaaa ttgatttttg taattgatga ttactccaat taccaatgaa taaattgata
    10501 ttggtttgtt ctagatgtgt gagtttggtt gtgtcgtaaa ctaaatttat aaattgtagt
    10561 tgatacatag ttttacctgc ctttaatcaa tatctatatt actttttagt aacctatcat
    10621 tgaaccatac cagttataat cacgattgaa tgataaatgt aataggcttg aatctgcatc
    10681 ttggattgat aattctgtat cccaaggatt ccagtaaata agtttttctt ggtcattaat
    10741 tttagcatta ccaacaactg ctagcgcatg tcctaaatgt gggtcattag ggttttgaga
    10801 tacactttgt gcaaggatca taattcctac attatctttt gtaagttgat caacttgttc
    10861 atatgatggt acgccttctt gataatgaat atctctgcct tgtgatttac cgtattcaat
    10921 catttgatta gggaatgttg cacaattagg aaggtcttgc tcacttactt cagggtataa
    10981 tgtacgcata atatcatgtg cattataagt gtctgtattt ttagttgcat ttaataatgc
    11041 tgccatactg aatcctgcac accatgagtt atcgaattgt tgttctctaa ttttgaagtt
    11101 ttttaatgta ttttcatatt gaacttgatc ttcttgtatc gcttcgtttt cttcaacttt
    11161 agtaggtgtt actgtatttt ttaactcttg tttcaatttt gctgaaactg ttttagcact
    11221 ttctttttct tttacattac caggtagagg cgtagctttt actaatctaa ctttgccatc
    11281 ttcttcaaaa taaaaccctt tttcatcagt aagaacagtg atatttgaat ttttatcttt
    11341 aatttggtct aaatctttag cgatgaagtt tgaaatttta acactgtaat tcatgtcttc
    11401 ttttgatttg tttaaatcat ctttattttt aggactaagt gttaaagtat aaacaatttt
    11461 accgtctttt ataactggat aatagtaact attatcttct tcaccattaa atttataaat
    11521 tttaaaagct tcgccaagtt catatttacc agtttttgca ttactttgtt tatctaatgc
    11581 tttagcataa ccagcaaatt gttgttgtgc tagatctttt actttttgag gtactttgtc
    11641 actcttaaca ttaatttcta gctgtttaga gtgtgaatcg gctttggctt tattgttatt
    11701 agcaaatgcg cctagtgata aaataagcat tgaaaccatt atgatgctta taatattgaa
    11761 tactctagat ttacatgaac tattcatata ataccctcca tttaaatcaa aatattttac
    11821 gttgctaaga ctttgcttct attttatcta gacgaactta attgttaatt aagctgcatc
    11881 tggattgtct gaattattgt tatcgccatt gtctggatta tcagggttgt tagggttgtc
    11941 agggttattt ggttcatctg ggttgttagg gttatcagga ttgttagggt tatcaggatt
    12001 atctgggtta ttaggttggt catcgttggc aaaatggata tcttcaatat tttgttttaa
    12061 gaagttgcgt acattttcat taataaatac cgcaccatta aattcatttg gtacaccgcc
    12121 ccaatgaatt ccgatcactt catttttttc attaaataca ggtgaacctg aattaccacc
    12181 agttgtactt aaatcatatt gcatagcttc gcctttgagg taagtgattt ttcctttact
    12241 ttcccacatt gttgctacag gtttatcacc aggatatcct gttacagtaa tattttggtt
    12301 aacttgtgtt tcagcattat tactcattgt tgctggttta actacttcac caatatgttt
    12361 gttttgctca ttaggggaga atttaactat tgctaaatca ccttcgcctg aatatttagt
    12421 gatttgttca gcagtgaaac caccatttgg ataattgtct tggttaattg cagaagggaa
    12481 tgcttttaaa gcatgaggat caccgtgcgt agcatctacg acgtgtttat ttgttaaaag
    12541 agtatcttta cctacaacta caccggaagc aataaatgta ccagtaggtg cttcaacttg
    12601 aatataagtt acgggtgcat aatgaccatt cgttgtatct gtgatttggt gacgatcgtt
    12661 atttggtaat ataacatttg cgtgttcacg ttgttctaat ggtttaaggt taccgccttt
    12721 ttgaatctta ggtgtttgct gtttgcttga ctgcgtttgt tgtggatgat tgtccatagc
    12781 ctttgaagat aacgcgtttg ctgctggaga actcacaagt gtcgctgttg tcaaagttgc
    12841 aacgaataaa gaactaactt ttaaaaattt acctttcatc taaaaacctc caaaaaattt
    12901 atttacaagt taaatataac actaaaaatt tttaagtcaa taagaatata taaataaatt
    12961 aaaaagtttt tgtaaagatt agagtttaca aaaacattaa gggaattaaa attgaattgt
    13021 attatacttt attcaattga gttaaattga taagatcgtc aattgtttgc atttatctaa
    13081 aagaataaat aagaaaggaa ttgagagcga atggaaagta tttgtttata ataataaata
    13141 gtaacttttg aataataatt gtttggccaa aggtagatta atatgaatgg tgatata
//
LOCUS       DANJFZ010000073        13151 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.073, whole genome shotgun sequence.
ACCESSION   DANJFZ010000073 DANJFZ010000000
VERSION     DANJFZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.073"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(167..1069)
                     /gene="cvfB"
                     /locus_tag="PWN45_001811"
     CDS             complement(167..1069)
                     /gene="cvfB"
                     /locus_tag="PWN45_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDL0623578.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1216..2817
                     /locus_tag="PWN45_001812"
     CDS             1216..2817
                     /locus_tag="PWN45_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0623579.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3677..4882
                     /locus_tag="PWN45_001813"
     CDS             3677..4882
                     /locus_tag="PWN45_001813"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL0623580.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4946..5935
                     /locus_tag="PWN45_001814"
     CDS             4946..5935
                     /locus_tag="PWN45_001814"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL0623581.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5937..6824
                     /locus_tag="PWN45_001815"
     CDS             5937..6824
                     /locus_tag="PWN45_001815"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL0623582.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6821..7543
                     /locus_tag="PWN45_001816"
     CDS             6821..7543
                     /locus_tag="PWN45_001816"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL0623583.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7570..8289
                     /gene="dapD"
                     /locus_tag="PWN45_001817"
     CDS             7570..8289
                     /gene="dapD"
                     /locus_tag="PWN45_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL0623584.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8432..9583
                     /locus_tag="PWN45_001818"
     CDS             8432..9583
                     /locus_tag="PWN45_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0623585.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9588..10673
                     /locus_tag="PWN45_001819"
     CDS             9588..10673
                     /locus_tag="PWN45_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL0623586.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10663..11928
                     /gene="lysA"
                     /locus_tag="PWN45_001820"
     CDS             10663..11928
                     /gene="lysA"
                     /locus_tag="PWN45_001820"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL0623587.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12168..12569)
                     /gene="msa"
                     /locus_tag="PWN45_001821"
     CDS             complement(12168..12569)
                     /gene="msa"
                     /locus_tag="PWN45_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDL0623588.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12766..12966)
                     /gene="cspA"
                     /locus_tag="PWN45_001822"
     CDS             complement(12766..12966)
                     /gene="cspA"
                     /locus_tag="PWN45_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDL0623589.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT         4607 a         2074 c         2342 g         4128 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct attcgtttga
       61 attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct gtccgattta
      121 atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat tcttttgagt
      181 ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata ttaataatct
      241 tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata ttgaatactt
      301 ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca tattcaacta
      361 gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt ggtaaaaatg
      421 aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca gattcaccta
      481 atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct gattcgctta
      541 ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact tcgtttttta
      601 aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc gctaaacgtc
      661 catacatatg attctcacgg tcaattcgta atgtaactag caaataatca ccaggttgtg
      721 gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca cggggtaatc
      781 caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag tcatatttat
      841 ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg tttggataaa
      901 tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt tctgattggt
      961 ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct tgtaacccta
     1021 ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg ttattctcct
     1081 tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat tacaacttat
     1141 atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata tcgaatgaaa
     1201 aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg agatcgtaaa
     1261 ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt aattggtgcg
     1321 aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga ttctcaaaca
     1381 ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga ccactatgct
     1441 tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct ttatgaggtt
     1501 atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga tggtatccgt
     1561 gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga agctgatgct
     1621 gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa aatggctgaa
     1681 ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg tgaaccagac
     1741 gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag ttggttagaa
     1801 gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg tcatttctta
     1861 aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt ttatgttggt
     1921 aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca agaacaaaac
     1981 aagaaaaaag aagaaaaaat gaaagagtta caggacttta ttgctcgttt ctcagctaac
     2041 gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat tgaattagat
     2101 gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga gcgcgaaatc
     2161 ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga aaaagtatta
     2221 gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg ggatagtgaa
     2281 attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga cgaaggttca
     2341 tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc agaattcttt
     2401 gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga agatgagcaa
     2461 actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga agaagttaag
     2521 aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag taaaatgatg
     2581 ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga cttagaaagt
     2641 attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt tacttcatat
     2701 gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa acaaggtggc
     2761 gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt aaaataaaca
     2821 taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac ttgcacatta
     2881 ttgtaagttg gcttactgtc accttctata ttgggactta ttccgcaact tgtattgttt
     2941 gtagaaatta ggaatccaat ttctctatgt gttgcaccga caaggattga aaaaagctag
     3001 ctacaaagtc attttcattc agtcaactac tgtcaatata acattgcagg gcctagggca
     3061 ttgattcatg tccgagacta tattcaattt aatagacatt aactgggcgt ctcgatgcta
     3121 atatttatag catcgaggtg tcctttttat tttatttttt aacacatcag catagtgaac
     3181 accccttctc taaattacaa atacagttat caacgcatta aaagagtaca tttatttaat
     3241 gtgcatattg attatgctat aagtattttg aattttattt ttcaaaaagt ctcattatca
     3301 ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc caaaaaatct
     3361 aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc aatttaataa
     3421 tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc taacagctaa
     3481 atttgaaagg gtgcgatgcc gaagcaatta taatagcagt tataatttgt tggacttttt
     3541 ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac ttgtatatag
     3601 attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg cccttcctaa
     3661 caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat catccgtcag
     3721 tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca atcaagatga
     3781 acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa tgacgaatgt
     3841 atcaaccttg actaaagcac caaaacaaca agaactggca ttattattga caaccggaga
     3901 gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtatga atgccaaagc
     3961 aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa gtaaaattgc
     4021 tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt tagtagttgc
     4081 tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag gtggttctga
     4141 tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt ataccgacgt
     4201 tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac tagacatcgt
     4261 ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg aaacaagaag
     4321 tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt tatcgaacgt
     4381 gaaaggaaca tggattatat caaatgaaga aatattagag aaaaaagcag ttactggtgt
     4441 ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg acaatcagct
     4501 acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata tgatttcaca
     4561 aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg attttcatca
     4621 aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt ataaaatcaa
     4681 tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt caggtgtggc
     4741 atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa caacaacatc
     4801 tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg tagaaaaact
     4861 atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa aaattggaaa
     4921 ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg caacaggatt
     4981 agtaggtaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg atgaattagt
     5041 attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaaggaa aaacatatac
     5101 agttcaagaa ttaactgatg ctcgtgcaag tgaacatttc gattatgtat taatgagtgc
     5161 tggtggcggt acaagcgaac actttgcccc actttttgaa aaagctggtg caatcgttat
     5221 agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc cggaagtcaa
     5281 tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga ttcaatctgt
     5341 tgtacctcta aaagtattgc aagatgctta tggtttaaaa cgagtggcat atacaacata
     5401 tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag gtgtaaatgg
     5461 taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc atattgatgt
     5521 gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga cgagaaaaat
     5581 tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg ttcaagatag
     5641 tcatagtgtt gaaattgatg taacgcttga caaagaaaca acagcagaag atattaaagc
     5701 gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca atgaatatcc
     5761 aatggcaatc aattctacta ataaagatga agtgtttgtt ggccgtatac gtagagatga
     5821 ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa aaggtgctgc
     5881 attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga attaaaatga
     5941 cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat aacaaagtta
     6001 atattgaagc tttgaaaaca cacgttaatt ttttactaga aaataatgcc caagcaatca
     6061 tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa gaacgcattc
     6121 taaaaacagt tattgatctt gtagataaac gtgttcctgt catagcagga actggcacta
     6181 atgatactga aaagtcaatc caagcttcaa tccaagctaa agccttaggg gctgatgcaa
     6241 ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc aaacactttg
     6301 aagcgattgc agatgctgtg aaattaccag tcgtgctgta caatgttcct tcaagaacga
     6361 acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat atagttgctt
     6421 taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcgc attgatacaa
     6481 attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat caacgtggcg
     6541 gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa gcgttatacg
     6601 atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc acactgttat
     6661 cagctttatc agttgatatt aacccaattc ctattaaagc tctaacaagt tatttaggat
     6721 ttggaaatta tgaattacgt ctaccattgg ttagcctaga agatacagat actaaagtgc
     6781 ttcgtgaaac atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat tactaattgg
     6841 ctatggcgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac atgaaatcgt
     6901 tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc aacatattgc
     6961 agatgttaaa ggtgccgatg ttgcaataga tttttcaaat ccaaatctgc ttttcccttt
     7021 attagatgaa gattttcatt tgccattagt tgtggcaaca actggcgaga aagaaaaact
     7081 acttaataag ttagatgaat tgagtcaaaa tatgcctgtg tttttcagcg cgaacatgag
     7141 ttatggcgtt catgcattga ctaaaatttt agcagctgct gttcccctac ttgatgattt
     7201 cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa gtggtacgtt
     7261 agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg tgtatgatag
     7321 acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta ttcgtggagg
     7381 tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga ttcaaatcac
     7441 gcatcgtgca caatcaaaag atatttttgc gaatggtgca atacaagcag cagaacgctt
     7501 agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt aaaggagatc
     7561 gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat aagtgatgct
     7621 aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat cacatatcca
     7681 gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga agcggatgat
     7741 tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat tgaaatggat
     7801 cgtcgcaatt ctgctattcc attaaaagac ttaacaaata cgaatgcacg aattgaacca
     7861 ggtgcgttta ttagagaaca agccattatt gaagatggtg ctgtcgttat gatgggcgca
     7921 acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa tgctactctc
     7981 ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt attagcaggt
     8041 gtgattgaac cccctagtgc ttcaccggtt ataatcgagg atgatgtatt aatcggtgca
     8101 aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc agctggcgcg
     8161 attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc aaaagtgatt
     8221 aagcaagctt ctgaagtaca agatactaaa aaagagattg tagcagcatt aagaaaactg
     8281 aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta tgtctcaacc
     8341 ttattgttta gcattgaata aacttatgat cacaaatttc gaaatacgtt acactattta
     8401 tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg ttacgaaaca
     8461 tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa caactgctta
     8521 tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg aaactggcgt
     8581 cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg atattgatgc
     8641 gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc atgtgatgca
     8701 tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac gttgcaaaga
     8761 catgcaagat gcaggtcaat taccgcaaaa tgtcgttttc attttccaac ctgcagaaga
     8821 aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt atccaattga
     8881 agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag tgataagaga
     8941 tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc tgtcaagtca
     9001 tgttgctgat aaagaacaag gtcattcttg tggtgaagca ttacaacatg tattaactca
     9061 aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg ttcatattgg
     9121 tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggctatt tagaaggtac
     9181 tattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc acaagatagc
     9241 agaaagtgtc aagcttctgt ttaatgtaga ttgtgaagtt aaatttgcag aaggttatcc
     9301 ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa taaaagctga
     9361 tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta gtttttatgg
     9421 tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag ataaaggttt
     9481 tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagtgttaa taaacgtggt
     9541 taatttttac gaaaatttat taaataatta caaagaggtg taatacattg acagcaacat
     9601 ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac aatcagccat
     9661 taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct gtaactcagt
     9721 ttatccatgc aggtatagat acatttagca caacatcact acgagaagca attcaaatta
     9781 gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag tttgatttag
     9841 tccgtgaaca tcaaatacat atgactttgc cgtcgttgac atattactat aaccataaaa
     9901 atgatttagc tggtattcat gttcacttag aatttgaaaa tttattacat cggtctggat
     9961 ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat caaaatgcaa
    10021 aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc gatgtgtcag
    10081 attataatgt tgaacgttca caatggatgg aaattgttga agcactttta tctgaaggtt
    10141 atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa ggacaaatat
    10201 tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca agaccatata
    10261 gttcactgaa tcaacatgat atagttcagt cattaacttt aaaagcacat gttattcaag
    10321 tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa gtgactaaaa
    10381 acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta agaactcgtg
    10441 ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta atgatgagcc
    10501 atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt ctttataata
    10561 atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct gaacaattaa
    10621 gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt taaatataat
    10681 caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca aagctttggt
    10741 acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg ttaccatcgc
    10801 gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc atttacttgc
    10861 attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt ttctgaaggt
    10921 gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt ccatggtaac
    10981 aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta ttttgttata
    11041 gattcattag aagaaattga attaatagac cgctatgcta atgatacggt tcaagttgta
    11101 ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac tgggcaagaa
    11161 gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat tgacaaagtc
    11221 caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc acagattgaa
    11281 ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa agagcaaggc
    11341 attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt tgaaggtgac
    11401 gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa atccgaaatt
    11461 aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc aattgtaggt
    11521 gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga gattaataaa
    11581 tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta tgacgcaaag
    11641 tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac tatagctgga
    11701 aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc atcagtcaaa
    11761 cgtggagact atcttgctat attatcaact ggtgcatatc attactctat ggcatccaat
    11821 tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc acgtgaagtt
    11881 ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa ataaacaaag
    11941 taatccccga agcacagaaa attatactgt gactcgggga atttttatgc attatagatg
    12001 actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac atttttagtt
    12061 agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc tattcaacta
    12121 atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta gattttatgc
    12181 tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata taatgtataa
    12241 aacagaattt catcaaatcc aaaatcttta cttgtaatta atacatcaat atcttctaat
    12301 tccatcatct ctgctttaat ataattatta aactttggca aagtcattaa atgtacattg
    12361 taaatatagt aataaaagtc gataaatatg atgttaaaaa atatgtatgt tttatcaata
    12421 ttagttttga aaaatagtat cccagaaatc attatcggaa tcactaatac aatcatcatt
    12481 gctgctaaaa tattaatatt taatccaatt gcagataata caccgaaaac taataaatta
    12541 gcaactaaac taaggataag atatttcata aaatcacctc gtcgttgtac accataatta
    12601 tattatataa atatattttt ctattgcaaa caatgttggt gatataaaaa aagagttctc
    12661 aatttggttg agaaactcat cgcgttaagt atttgtaata agcatataaa aacaccttac
    12721 ttcttggtaa ggtgttagtt aaaatcaatg attcaaatct aagaattata gtttaacaac
    12781 gtttgcagct tgtggaccgc ggtcgccttc aactacttca aactcaacag cttgaccttc
    12841 ttctaatgat ttgtaaccat cttggttaat tgctgaaaaa tgtacgaata cgtcattttc
    12901 tccttcaact tcgataaagc cgaatccttt ttcagcgtta aaccatttaa ctgtaccttg
    12961 tttcataatc tgaaacctcc aagactaaaa ttcattcaat atgcttattc gcatattaca
    13021 aaaaatacat gcaaatttca ctcaaatatt ctttgcaata tggaataatc atctgcttaa
    13081 cttgtattat agtgcagtta ttaccaaatt gcaatacaat aataaaaaat attcatttaa
    13141 tcaattattg g
//
LOCUS       DANJFZ010000074        12866 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.074, whole genome shotgun sequence.
ACCESSION   DANJFZ010000074 DANJFZ010000000
VERSION     DANJFZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.074"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..524
                     /locus_tag="PWN45_001823"
     CDS             <1..524
                     /locus_tag="PWN45_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL0623590.1"
                     /translation="VVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQI
                     ALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNRI
                     ISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAKV
                     VLNANDIFEDYYI"
     gene            698..2773
                     /gene="topA"
                     /locus_tag="PWN45_001824"
     CDS             698..2773
                     /gene="topA"
                     /locus_tag="PWN45_001824"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL0623591.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2929..4236
                     /gene="trmFO"
                     /locus_tag="PWN45_001825"
     CDS             2929..4236
                     /gene="trmFO"
                     /locus_tag="PWN45_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDL0623592.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            4653..5549
                     /gene="xerC"
                     /locus_tag="PWN45_001826"
     CDS             4653..5549
                     /gene="xerC"
                     /locus_tag="PWN45_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL0623593.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            5546..6091
                     /gene="hslV"
                     /locus_tag="PWN45_001827"
     CDS             5546..6091
                     /gene="hslV"
                     /locus_tag="PWN45_001827"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL0623594.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            6157..7560
                     /gene="hslU"
                     /locus_tag="PWN45_001828"
     CDS             6157..7560
                     /gene="hslU"
                     /locus_tag="PWN45_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL0623595.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            7585..8358
                     /gene="codY"
                     /locus_tag="PWN45_001829"
     CDS             7585..8358
                     /gene="codY"
                     /locus_tag="PWN45_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL0623596.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            8409..8501
                     /locus_tag="PWN45_001830"
     CDS             8409..8501
                     /locus_tag="PWN45_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623597.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            8700..9467
                     /gene="rpsB"
                     /locus_tag="PWN45_001831"
     CDS             8700..9467
                     /gene="rpsB"
                     /locus_tag="PWN45_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL0623598.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            9501..9614
                     /locus_tag="PWN45_001832"
     CDS             9501..9614
                     /locus_tag="PWN45_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623599.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            9649..10530
                     /locus_tag="PWN45_001833"
     CDS             9649..10530
                     /locus_tag="PWN45_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL0623600.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            10667..11389
                     /locus_tag="PWN45_001834"
     CDS             10667..11389
                     /locus_tag="PWN45_001834"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL0623601.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            11408..11962
                     /gene="frr"
                     /locus_tag="PWN45_001835"
     CDS             11408..11962
                     /gene="frr"
                     /locus_tag="PWN45_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL0623602.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            12335..>12866
                     /gene="uppS"
                     /locus_tag="PWN45_001836"
     CDS             12335..>12866
                     /gene="uppS"
                     /locus_tag="PWN45_001836"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL0623603.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSD"
BASE COUNT         4889 a         1824 c         2490 g         3663 t
ORIGIN      
        1 cagtagtagg tgcaagagat tctacaagtt atacccaaca gtctttagaa tttttattat
       61 caaatgataa aagcaaatat ttaacaattg tttccggcct tgctcaagga gctgatgcaa
      121 tggcacatca aatagcttta aaatacaatc tccctacaat tgcagtttta gcctttggcc
      181 atcaaacaca ttatcccaaa agtacattag cattaagaaa taaaatagaa gaaaaaggtt
      241 tagttatatc tgaatatcca ccacatacac caatcgctaa atatagattt cctgagcgca
      301 atagaattat cagcggtttg tcaaaagggg ttttaattac tgaggctaag gaacaaagtg
      361 gcagtcacat cacgatagat tttgcattag agcaaaatag aaatgtttat gttttacctg
      421 gatctatgtt taatcctatg acaaaaggta atttattacg tatccaagaa ggtgctaagg
      481 tagtattaaa cgctaatgat atatttgaag actactatat ttaaaactaa aaataacgag
      541 ttgcttcata tttcttttgt ttccctttgt aatttatttt ttaataaaat ctataaaaaa
      601 tagacaggga aaatgatttg tttagatata aaacgttgac aaaagcaaaa ttaagcgttt
      661 atcatttatc tttagtaatt agattagcga gggggaaatg acattggcag ataatttagt
      721 cattgttgaa tcgcctgcaa aagcaaaaac cattgaaaag tatttaggta agaaatataa
      781 agttatagct tcaatgggac acgtcagaga cttaccaaga agtcaaatgg gtgtcgacac
      841 tgaagataat tacgaaccaa aatatataac aatacgcgga aaaggtcctg ttgtaaaaga
      901 attgaaaaaa catgcaaaaa aagcgaaaaa cgtctttctc gcaagtgacc ccgaccgtga
      961 aggtgaagca attgcttggc atttatcaaa aattttagag cttgaagatt ctaaagaaaa
     1021 tcgcgttgtt ttcaacgaaa taactaaaga cgctgttaaa gaaagtttta aaaatcctag
     1081 agaaattgaa atgaacttag tcgatgcaca acaagcgcgt cgaatattag atagattagt
     1141 tggctataac atctcgccag ttctttggaa aaaagtaaaa aaagggttgt cagcgggtcg
     1201 agttcaatct gttgcacttc gtttagtcat tgaccgtgaa aatgaaattc gaaactttaa
     1261 accagaagaa tattggacta ttgaaggaga atttagatac aaaaaatcaa aattcaatgc
     1321 taaattcctt cattataaaa ataaaccttt taaactaaaa acgaaaaaag atgttgagaa
     1381 aattacagct gcattagatg gagatcaatt cgaaattaca aacgtgacta aaaaagaaaa
     1441 aacgcgtaat ccagcaaacc catttacaac ttctacatta caacaagagg cggcacgtaa
     1501 attaaacttt aaagcaagaa aaacaatgat ggtcgcacaa caattatatg aaggtataga
     1561 tttgaaaaaa caaggtacga ttggtttaat aacatatatg agaaccgatt ctacacgtat
     1621 ttcagatact gccaaagttg aagcaaaaca gtatataact gataaatacg gtgaatctta
     1681 cacttctaaa cgtaaagcat cagggaaaca aggtgaccaa gatgcccatg aggctattag
     1741 accttcaagt actatgcgta cgccagatga tatgaagtca tttttgacga aagaccaata
     1801 ccgattatac aaattaattt gggaacgatt tgttgctagt caaatggctc cagcaatact
     1861 tgatacagtc tcattagaca taacacaagg tgacattaaa tttagagcga atggtcaaac
     1921 aatcaagttt aaaggattta tgacacttta tgtagaaact aaagatgata gtgatagcga
     1981 aaaggaaaat aaactgccta aattagagca aggtgataaa gtcacagcaa ctcaaattga
     2041 accagctcaa cactatacac aaccacctcc aagatatact gaggcgagat tagtaaaaac
     2101 actagaagaa ttgaaaattg ggcgaccatc aacttatgca ccgacaatag atacgattca
     2161 aaagcgtaac tatgtcaaat tagaaagtaa gcgttttgtt cctactgagt tgggagaaat
     2221 agttcatgaa caagtgaaag aatacttccc agagattatt gatgtggaat tcacagtgaa
     2281 tatggaaacg ttacttgata agattgcaga aggcgacatt acatggagga aagtaatcga
     2341 cggtttcttt agtagcttta aacaagatgt tgaacgtgct gaagaagaga tggaaaagat
     2401 tgaaatcaaa gatgagccag ccggtgaaga ctgtgaaatt tgtggttctc ctatggttat
     2461 aaaaatggga cgctatggta agttcatggc ttgctcaaac ttcccggatt gtcgtaatac
     2521 aaaagcgata gttaagtcta ttggtgttaa atgtccaaaa tgtaatgatg gtgacgtcgt
     2581 agaaagaaaa tctaaaaaga atcgtgtctt ttatggatgt tcgaaatatc ctgaatgcga
     2641 ctttatctct tgggataagc cgattggaag agattgtcca aaatgtaacc aatatcttgt
     2701 tgaaaataaa aaaggcaaga caacacaagt aatatgttca aattgcgatt ataaagaggc
     2761 agcgcagaaa taatattttt atttcctaga tacattttaa gattgttaaa tagaatcatt
     2821 agtgaatctt attttaaaga tagtaaagga ttaatctaaa taagtgcgga taatataaac
     2881 ataacaacat aattaaaaga cataaatgac aataaaagga gtatagaaat gactcaaact
     2941 gtaaatgtaa taggtgctgg tcttgccggt tcagaagcgg catatcaatt agctgaaaga
     3001 ggaattaaag ttaatctaat agagatgaga cctgttaaac aaacaccagc gcaccatact
     3061 gataaatttg cggaacttgt atgttccaat tcattacgcg gaaatgcttt aactaatggt
     3121 gtgggtgttt taaaagaaga aatgagaaga ttgaattcta taattattga agcggctgat
     3181 aaggcacgag ttccagctgg tggtgcatta gcagttgata gacacgattt ttcaggttat
     3241 attactgaaa cacttaaaaa tcatgaaaat atcacagtta ttaatgaaga aattaatgcc
     3301 attccagatg gatacacaat tatcgcaaca ggaccactta ctacagaaac ccttgcgcaa
     3361 gaaatagtgg acattactgg taaagatcaa ctttatttct atgatgcggc tgctccaatt
     3421 attgaaaaag aatctattga tatggataaa gtttacttaa agtcccgtta tgataaaggt
     3481 gaagctgcat atttaaactg tcctatgact gaggatgaat ttaatcgctt ttatgatgca
     3541 gtattagaag ctgaagttgc gcctgtaaat tcatttgaaa aagaaaaata tttcgagggt
     3601 tgtatgcctt ttgaagtaat ggcagaacgc ggacgcaaga cattactatt tggaccaatg
     3661 aaaccagtag gattagaaga tccaaagact gggaaacgtc cttatgcggt ggttcaatta
     3721 agacaagatg acgctgctgg tacactctac aatattgttg gcttccaaac gcatttaaaa
     3781 tggggagctc aaaaagaagt cattaaatta attccaggct tagaaaatgt tgatattgtt
     3841 agatatggtg tgatgcatag aaataccttc attaattcac cggacgtatt aaacgagaaa
     3901 tatgaattga tttcacaacc aaacatacag tttgcgggac aaatgactgg tgttgaaggt
     3961 tatgtagaaa gcgcagctag cggcttagtt gcaggtatca atcttgcgca taaaatatta
     4021 ggcaagggtg aggtagtatt tccgagagaa acaatgattg gaagtatggc ttactatatt
     4081 tctcatgcta aaaacaataa gaatttccaa cctatgaatg ctaacttcgg gttattacca
     4141 tctttagaaa ctagaattaa agataaaaaa gaacgctatg aagcacaagc taatagagct
     4201 ttggattact tagaaaattt caaaaaaact ttataaaata gttagaaaga ctagatatgc
     4261 tattcattct taagtcatca acgagtaagt aatgactttc taaatggaaa atacttatcc
     4321 tagtcttttt aattttggaa ttgttacgta tttctgacaa tttagaattc gcattcaaaa
     4381 aatatctaaa taaataacac gcaataagtt gattgatgta acatgtaaga gaatgtttta
     4441 aataaacttt atttaaaagg caatgaaata ataaatggca aggctattaa taaagacttt
     4501 tagtaattaa tttaaaaaag aggtattcta attaacaggt tttccgatta gttacaatta
     4561 tttaattctc aaaagattta gaattgatta tcaaattacc gtaagccctt tgctgtatat
     4621 gctacaattc ttattgatgg agggtaaatg tattgaatca tattcaagat gcgtttttaa
     4681 atacattgaa agttgaacgg aatttttcgg aacacacatt gaaatcatat caagatgact
     4741 taattcagtt taatcaattt ttagaacaag aacatttaga gttgaatact tttgaataca
     4801 gagatgctag aaattatttg agctatttat attcaaatca tttgaaaaga acatctgttt
     4861 ctcgtaaaat ctcaacgtta agaactttct atgaatattg gatgacgctt gatgagaaca
     4921 ttattaatcc atttgttcaa ttagtacatc cgaaaaaaga aaaatatctt ccgcaattct
     4981 tttacgaaga agaaatggaa gcgttattca aaactgtaga agaggacact tcaaaaaatt
     5041 tacgggatcg agttattctt gaattgttgt atgctacagg catccgtgtt tcggaattag
     5101 taaatattaa aaaacaagat atagattttt acgcgaatgg tgttaccgta ttaggaaaag
     5161 ggagcaaaga gcgctttgta ccgtttggtg cttattgtag acaaagcatc gaaaattatt
     5221 tagaacattt caaaccaatt cagtcatgca atcatgattt tcttattgta aatatgaagg
     5281 gtgaagcaat cactgaacgc ggtgtacgat atgttttaaa tgatattgtt aaacgaacag
     5341 caggcgtaag tgagattcat ccccacaagc tcagacatac atttgcaacg catttattga
     5401 atcaaggtgc agacctaaga acagtacaat cgttattagg tcatgttaat ttgtcaacaa
     5461 ctggtaaata tacacacgta tctaaccaac aattaagaaa agtgtatcta aatgcacatc
     5521 ctcgagcgaa aaaggagaat gaaacatgag taatacaaca ttacatgcaa caacaattta
     5581 tgctgtaaga cataatggga aagcagctat ggctggagat gggcaagtaa cgcttggtca
     5641 acaagtcatc atgaaacaaa cggcaagaaa agtgcgacgt ttatatgaag gtaaagtgtt
     5701 agctggtttc gcaggtagtg tagcagatgc gtttacgtta tttgaaaaat tcgaaacaaa
     5761 attacaacag tttagtggta acttagaaag agctgctgtt gaattggcac aagaatggcg
     5821 aggcgataaa caattacgtc aattagaagc tatgctaatt gtaatggata aagatgctat
     5881 tttagttgtc agtggaactg gcgaagttat tgctccagat gatgacctta tcgctattgg
     5941 atcaggaggc aactacgcat taagcgcagg acgtgcattg aaacgccatg catcgcattt
     6001 gtctgctgaa gaaatggcat atgagagctt gaaagtagcg gctgatattt gtgtctttac
     6061 caacgataat attgttgtcg aaacactata ataatcagag cacgataaat aattacgagc
     6121 aattaatttt agttaaaaga cggaggaatg aaattaatgg atacagctgg aataagatta
     6181 actccaaaag aaatcgtatc taaattaaat gaatacatcg ttggacaaaa tgatgctaaa
     6241 cgtaaagtgg caattgccct acgtaatcga tacagaagaa gtttattaga tgaggaatca
     6301 aagcaagaaa tttcacctaa aaatattttg atgattggac caaccggcgt tggtaaaact
     6361 gaaattgcaa gaagaatggc caaagttgtc ggcgcgccat ttataaaagt agaagctact
     6421 aaatttactg aggtaggtta tgtaggacga gatgttgaaa gtatggttag agatcttgtt
     6481 gatgtttcag taagattagt caaggcgcag aaaaaatcat tggtacaaga tgaagcaaca
     6541 gctaaggcca atgaaaaact tgttaagtta ttagttccaa gtatgaaaaa gaaagcgtct
     6601 caaacgaata atcctttaga gtcacttttc ggaggtgcaa ttccaaattt cggacaaaat
     6661 aacgaagatg aagaagaacc acctactgag gaaattaaaa caaaacgttc tgaaattaag
     6721 agacagctag aagaaggcaa acttgaaaaa gaaaaggtaa gaattaaagt cgaacaagat
     6781 cctggtgctt taggtatgct aggtacaaat caaaatcagc aaatgcaaga gatgatgaat
     6841 caattaatgc ctaaaaagaa agttgagcga gaagttgctg ttgagacggc aaggaaaatc
     6901 ttagctgata gttatgcgga tgaactaatt gatcaagaaa gcgctaacca agaagcgctt
     6961 gaattagcag aacaaatggg tatcatcttt atagatgaaa tcgacaaagt tgcgacgaat
     7021 aatcataata gtggtcaaga tgtctcaaga caaggtgttc aaagagatat tttacctata
     7081 cttgaaggta gcgttattca aaccaaatat ggtactgtga atactgaaca tatgctgttt
     7141 ataggtgctg gagctttcca tgtatctaag ccgagtgact tgataccaga attgcaaggt
     7201 cgttttccga ttagagttga acttgatagt ttatcggtag aagattttgt aagaattttg
     7261 acagaaccaa aattgtcatt aattaaacaa tatgaagcat tgcttcaaac agaagaagtt
     7321 actgtaaact ttaccgatga agcaattact cgcttagctg agattgctta tcaagtaaat
     7381 caagatacag acaacattgg tgcacgtcga cttcatacaa ttttagaaaa gatgctagaa
     7441 gatttatcat tcgaagcacc aagtatgccg aatgcagttg tagatattac cccacaatat
     7501 gttgatgata aattaaaatc aatttcaaca aataaagatt taagtgcatt tattctataa
     7561 aaatatacaa aaggagaaaa attcatgagc ttattatcta aaacgagaga gttaaacacg
     7621 ttacttcaaa aacacaaagg tattgcggtt gattttaaag atgtagcaca aacgattagt
     7681 agcgtaactg taacaaatgt atttattgta tcgcgtcgag gtaaaatttt aggatcgagt
     7741 ctaaatgaat tattaaaaag tcaaagaatt attcaaatgt tggaagaaag acatattcca
     7801 agtgaatata cagaacgatt aatggaagtt aaacaaacag aatcaaatat tgatatcgac
     7861 aatgtattaa cagtattccc acctgaaaac agagaattat tcatagatag tcgtacaact
     7921 atcttcccaa ttttaggtgg aggggaaaga ttaggtacat tagtacttgg tcgagtacat
     7981 gatgatttta atgaaaatga tttggtacta ggtgaatatg ctgctacagt tattggtatg
     8041 gaaatcttac gtgagaagca tagtgaagta gaaaaagaag cgcgcgataa agctgctatt
     8101 acaatggcaa ttaattcatt atcttattct gaaaaagaag cgattgaaca tatctttgaa
     8161 gaacttggcg gtacggaagg cctattaatc gcatcaaaag ttgcagatag agttggtatt
     8221 actagatctg taattgtaaa tgcactacgt aaattagaaa gtgctggtgt aattgaatca
     8281 cgttctttag gaatgaaagg tactttcatt aaagttaaaa aagaaaaatt cttagatgaa
     8341 ttagaaaaaa gtaaataagt cgatgagtct gggacataat tcaatgtcct agtcgtgacg
     8401 atgttatctt ggcggtagtc aatgaaatga ctttgatgat tatgttgaag tcatttcttt
     8461 atttttgtct aattttcaat caaattgata tttcacatta acttgataga attaccttta
     8521 aatttatgga aaaatgcttg aaaaacattt gctttagaca cggcattatg ttatatttat
     8581 tttcggctat aaagccaata cacacatatc aaacgtgatt gtgtaaaggg tgcaatattt
     8641 atatattgtc tttacaatag tttgctatgg aggtaattaa ccaataggag gaatttataa
     8701 tggcagtaat ttcaatgaaa caattactag aagcgggtgt tcacttcggt caccaaacac
     8761 gtcgttggaa cccaaaaatg aaaaaatata tcttcactga gagaaatggt atttatatca
     8821 tcgacttaca aaaaacagtg aaaaaagtag acgaggcata caacttcttg aaacaagttt
     8881 cagaagatgg tggacaagtc ttattcgtag gaactaaaaa acaagcacaa gaatcagtta
     8941 aatctgaagc agaacgtgct ggtcaattct acattaacca aagatggtta ggtggattat
     9001 taactaacta taaaacgatc tcaaaacgaa tcaaacgtat ttctgaaatt gaaaaaatgg
     9061 aagaagatgg tttattcgaa gtattaccta aaaaagaagt agtagaactt aaaaaagaat
     9121 acgaccgttt aatcaaattc ttaggcggaa ttcgtgatat gaaatcaatg cctcaagcat
     9181 tattcgtagt tgacccacgt aaagagcgta atgcaattgc tgaagctcgt aaattaaata
     9241 ttcctatcgt aggtatcgtt gacactaact gtgatcctga cgaaattgac tacgttatcc
     9301 cagcaaacga cgatgctatc cgtgcggtta aattattaac tgctaaaatg gcagatgcaa
     9361 tcttagaagg tcaacaaggc gtttctaatg aagaagtagc tgcagaacaa aacatcgatt
     9421 tagatgaaaa agaaaaatca gaagaaacag aagcaactga agaataatca actgttgaat
     9481 ctgacttaga tatagtttaa atgggtgata agatattaat gcttatcacc ttttttaaaa
     9541 agaaaatcga ggcaaattac aaatattcaa ttagagtatt ggcaatcttg cctataataa
     9601 tgctaaaatc ataatatata aaatgataac ttattggagg aataatgaat ggcaactatt
     9661 tcagcaaaac ttgttaaaga attacgtgaa aaaactggcg cgggtatgat ggattgtaaa
     9721 aaagcgctaa ctgaaactga tggtgacatc gataaagcga ttgactacct acgtgaaaaa
     9781 ggtattgcta aagcagctaa aaaagcagac cgtattgcgg ctgaaggttt agtacatgta
     9841 gaaactaaag gtaacgacgc agttatcgtt gaaatcaact ctgaaacaga ctttgttgct
     9901 cgtaacgaag gtttccaaga gttagttaaa gaaatcgcta atcaagtatt agatacaaaa
     9961 gctgaaactg ttgaagcttt aatggaaaca actttaccaa atggtaaatc agttgatgaa
    10021 agaattaaag aagcaatttc aacaatcggt gaaaaattaa gtgttcgtcg ttttgctatc
    10081 agaactaaaa ctgataacga tgctttcggc gcttacttac acatgggtgg acgcattggt
    10141 gtattaacag ttgttgaagg ttcaactgac gaagaagcag caagagacgt tgctatgcat
    10201 atcgctgcaa tcaaccctaa atatgtttct tctgaacaag ttagcgaaga agaaatcaac
    10261 cacgaaagag aagttttaaa acaacaagca ttaaatgaag gtaaaccaga aaacatcgtt
    10321 gaaaaaatgg tggaaggacg tttacgtaaa tacttacaag aaatttgtgc tgtagatcaa
    10381 gacttcgtta aaaaccctga tgtaacagtt gaagctttct taaaaacaaa aggtggaaaa
    10441 cttgttgact tcgtacgcta tgaagtaggc gaaggtatgg aaaaacgcga agaaaacttt
    10501 gcggatgaag ttaaaggaca aatgaaataa tctgtcataa agtaaaacaa ggaagaagac
    10561 acctttaatg ttgctttatt aaaatgtaaa tcattctaat aaaacgacaa ctgtgtcttc
    10621 tttacttgta tatgttacat atattcacga tagagaggat aagaaaatgg ctcaaatttc
    10681 taaatataaa cgtgtagttt tgaaactaag tggtgaagcg ttagctggag aaaaaggatt
    10741 tggcataaat ccagtaatta ttaaaagtgt tgctgagcaa gtggctgaag ttgctaaaat
    10801 ggactgtgaa atcgcagtaa tcgttggtgg cggaaacatt tggagaggta aaacaggtag
    10861 tgacttaggt atggaccgtg gaactgctga ttacatgggt atgcttgcaa ctgtaatgaa
    10921 tgccttagca ttacaagata gtttagaaca attggattgt gatacacgag tattaacatc
    10981 tattgaaatg aagcaagtgg ctgaacctta tattcgtcgt cgtgcaatta gacacttaga
    11041 aaagaaacgc gtagttattt ttgctgcagg tattggaaac ccatacttct ctacagatac
    11101 tacagcggca ttacgtgctg cagaagttga agcagatgtt attttaatgg gcaaaaataa
    11161 tgtagatggt gtatattctg cagatcctaa agtaaacaaa gatgcggtaa aatatgaaca
    11221 tttaacgcat attcaaatgc ttcaagaagg tttacaagta atggattcaa cagcatcctc
    11281 attctgtatg gataataaca ttccgttaac tgttttctct attatggaag aaggaaatat
    11341 taaacgtgct gttatgggtg aaaagatagg tacgttaatt acaaaataaa tttagaggtg
    11401 taaaataatg agtgacatta ttaatgaaac taaatcaaga atgcaaaaat caatcgaaag
    11461 cttatcacgt gaattagcta acatcagtgc aggaagagct aattcaaatt tattaaacgg
    11521 cgtaacagtt gattactatg gtgcaccaac acctgtacaa caattagcaa gcatcaatgt
    11581 tccagaagca cgtttacttg ttatttctcc atacgacaaa acttctgtag ctgacatcga
    11641 aaaagcgata atagcagcta acttaggtgt taacccaaca agtgatggtg aagtgatacg
    11701 tattgctgta cctgccttaa cagaagaacg tagaaaagag cgcgttaaag atgttaagaa
    11761 aattggtgaa gaagctaaag tatctgttcg aaatattcgt cgtgatatga atgatcagtt
    11821 gaaaaaagat gaaaaaaatg gcgacattac tgaagatgag ttgagaagtg gtactgaaga
    11881 tgttcagaaa gcaacagaca attcaataaa agaaattgat caaatgattg ctgataaaga
    11941 aaaagatatt atgtcagtat aaaactaata tacaatgaca tattaaaatg ccagtattaa
    12001 acgataatgt aacatttaaa atgggcatgt ttaattaaat caaagatgca tgtgataatt
    12061 taaattcaca atgagcataa aaatggtgtt taaacaagtt aattaaacat atactttata
    12121 aataataggc attaggtata ttgctataat aaagttatgt aatttttaac ctcagtatgt
    12181 atgtcacatt tctggtgtaa actgtaccga gtcagacttt ggtacagttt ttttatttgc
    12241 ttattcaatg cattaaatga gtatgataaa atgataatga ttgtttagta acttatacta
    12301 tatgacagag atgatcaggc tcggaggaaa gaccatgttt aaaaagctaa taaataaaaa
    12361 gaacactata aataattata atgaagaatt agactcgtct aatatacctg aacatatcgc
    12421 tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta gaattaaagg
    12481 tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg atattggtgt
    12541 taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg aaagtgaagt
    12601 aaattatatt atgaatttgc ctgtcaattt cttaaagaca ttcttaccgg aactaattga
    12661 aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat caacgataga
    12721 agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat taatatttgc
    12781 aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt ttgacgagct
    12841 tcatcaacaa ggtttaaata gtgata
//
LOCUS       DANJFZ010000075        12601 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.075, whole genome shotgun sequence.
ACCESSION   DANJFZ010000075 DANJFZ010000000
VERSION     DANJFZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.075"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            13..942
                     /gene="hlgA"
                     /locus_tag="PWN45_001837"
     CDS             13..942
                     /gene="hlgA"
                     /locus_tag="PWN45_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDL0623604.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            1509..2456
                     /gene="hlgC"
                     /locus_tag="PWN45_001838"
     CDS             1509..2456
                     /gene="hlgC"
                     /locus_tag="PWN45_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDL0623605.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            2458..3435
                     /gene="hlgB"
                     /locus_tag="PWN45_001839"
     CDS             2458..3435
                     /gene="hlgB"
                     /locus_tag="PWN45_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDL0623606.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(3487..3954)
                     /locus_tag="PWN45_001840"
     CDS             complement(3487..3954)
                     /locus_tag="PWN45_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0623607.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(3965..4657)
                     /locus_tag="PWN45_001841"
     CDS             complement(3965..4657)
                     /locus_tag="PWN45_001841"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDL0623608.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(4668..5783)
                     /locus_tag="PWN45_001842"
     CDS             complement(4668..5783)
                     /locus_tag="PWN45_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDL0623609.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(5761..6768)
                     /gene="bioB"
                     /locus_tag="PWN45_001843"
     CDS             complement(5761..6768)
                     /gene="bioB"
                     /locus_tag="PWN45_001843"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL0623610.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(6770..8128)
                     /gene="bioA"
                     /locus_tag="PWN45_001844"
     CDS             complement(6770..8128)
                     /gene="bioA"
                     /locus_tag="PWN45_001844"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL0623611.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(8106..8792)
                     /gene="bioD"
                     /locus_tag="PWN45_001845"
     CDS             complement(8106..8792)
                     /gene="bioD"
                     /locus_tag="PWN45_001845"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL0623612.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(8846..9013)
                     /locus_tag="PWN45_001846"
     CDS             complement(8846..9013)
                     /locus_tag="PWN45_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623613.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(9244..10977)
                     /locus_tag="PWN45_001847"
     CDS             complement(9244..10977)
                     /locus_tag="PWN45_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623614.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(11002..>12601)
                     /locus_tag="PWN45_001848"
     CDS             complement(11002..>12601)
                     /locus_tag="PWN45_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623615.1"
                     /translation="TINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKK
                     LAQKLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIM
                     LIDWRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKT
                     FNQSQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISIN
                     QLNYAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKK
                     PQHYEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYW
                     DVTSGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEK
                     AAKLAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVY
                     VDPDNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKL
                     NGNYKKMWDTHMHTKDWGINTGHN"
BASE COUNT         4301 a         2246 c         1716 g         4338 t
ORIGIN      
        1 aaagtgattt ctatgattaa aaataaaata ttaacagcaa ctttagcagt tggtttaata
       61 gcccctttag ccaatccatt tatagaaatt tctaaagcag aaaataagat agaagatatc
      121 ggccaaggtg cagaaatcat caaaagaaca caagacatta ctagcaaacg attagctata
      181 actcaaaaca ttcaatttga ttttgtaaaa gataaaaaat ataacaaaga tgccctagtt
      241 gttaagatgc aaggcttcat tagctctaga acaacatatt cagacttaaa aaaatatcca
      301 tatattaaaa gaatgatatg gccatttcaa tataatatca gtttgaaaac gaaagactct
      361 aatgttgatt taattaatta tcttcctaaa aataaaattg attcagcaga tgttagtcag
      421 aaattaggct ataatatcgg cggaaacttc caatcagcgc catcaatcgg aggcagtggc
      481 tcattcaact actctaaaac aattagttat aatcaaaaaa actatgttac tgaagtagaa
      541 agtcagaact ctaaaggtgt taaatgggga gtgaaagcaa attcattcgt tacaccgaat
      601 ggtcaagtat ctgcatatga tcaatactta tttgcacaag acccaactgg tccagcagca
      661 cgagactatt tcgtcccaga taatcaacta cctcctttaa ttcaaagtgg ctttaatcca
      721 tcatttatta caacattgtc acacgaaaga ggtaaaggtg ataaaagcga gtttgaaatc
      781 acttacggca gaaacatgga tgctacatat gcttacgtga caagacatcg tttagccgtt
      841 gatagaaaac atgatgcttt taaaaaccga aacgttacag ttaaatatga agtgaactgg
      901 aaaacacatg aagtaaaaat taaaagcatc acacctaagt aaacagttca atcatcttaa
      961 aaaatcctgg gacacttcat acttgtctca ggatttttta acaaattgaa tcagccttat
     1021 aacattaaat tattttatcg tacattaaat ttaataataa caactgattt ttataagaat
     1081 aaagtatcga accatagtag atacacaaat aatacaaatg aaacaattta acttgaaagc
     1141 ttaaataaat attatcaagt taataaacaa ttaattttta gatggattca tcaaaaatcg
     1201 taaaaagcac aatttgtatt ttacaaacat taattaaaaa agaaagcaag acattcgtgc
     1261 aatcggttac cttaaattgt ttacaactgt caacaatacc aaggttttat taactatatt
     1321 tctcacaaaa ttagctttta gcattccaaa caaaaaaggt taaatcgaac ggaattatgg
     1381 catttttaac ttaattgtaa aaaaagttga taatggtcaa ttgttaatga acagttaatt
     1441 ataataacgc ccaaaatata ttattattta attaagttaa ataaaattat agaaataaag
     1501 tgaaacttat gcttaaaaat aaaatattaa ctacaacttt atctgtgagc ttacttgccc
     1561 ctcttgccaa tccgttatta gaaaatgcta aagctgctaa cgatactgaa gacatcggta
     1621 aaggaagcga tatagaaatt atcaaaagga cagaagataa aacaagtaat aaatggggcg
     1681 tgactcaaaa tattcaattt gattttgtaa aggataaaaa atataacaaa gatgctttga
     1741 tattaaagat gcaaggattc attagctcta gaacaacata ttacaactat aaaaaaacta
     1801 atcatgttaa agctatgcga tggccattcc aatataatat tggtttaaaa acaaatgata
     1861 aatatgtttc tttaattaat tatttaccta aaaataaaat tgaatctaca aacgtgagtc
     1921 agacattagg atacaatatc ggtggtaatt tccaatcagc cccatcactc ggtggtaatg
     1981 gatcatttaa ctattctaaa tcgattagct atacacaaca aaattatgta agtgaagtag
     2041 aacaacaaaa ctcaaaaagt gttttatggg gcgtcaaagc gaattcattc gccactgaat
     2101 caggtcaaaa atcagccttt gatagcgatt tatttgtagg ctacaaacct catagtaaag
     2161 atcctagaga ttatttcgtt ccagacagtg agttaccacc tcttgtacaa agtggattta
     2221 acccttcatt tatcgccaca gtatctcatg aaaaaggttc aagcgataca agcgaatttg
     2281 aaattactta cggaagaaac atggatgtca ctcatgccat taaaagatca acgcattatg
     2341 gcaacagtta tttagacgga catagagtcc ataatgcatt tgtaaataga aactatactg
     2401 tgaaatacga ggtcaattgg aagactcatg aaatcaaggt gaaaggacag aattgatatg
     2461 aaaatgaata aattagtcaa atcatccgtt gctacatcta tggcattatt attactttct
     2521 ggtactgcta atgctgaagg taaaataaca ccagtcagcg taaaaaaagt cgatgacaaa
     2581 gttactttat acaaaacaac agccacagca gattctgata aatttaaaat ttcacagatt
     2641 ttaacattta atttcatcaa agataaaagt tatgataaag atactttagt acttaaagct
     2701 actgggaata ttaactcagg ctttgtgaaa cctaatccta atgactatga cttttcaaaa
     2761 ttatattggg gagctaaata caatgtatct ataagctcac aatctaatga ttcagtaaac
     2821 gtcgttgatt atgcaccaaa aaatcaaaat gaagagtttc aagttcaaaa tactttaggc
     2881 tatacatttg gtggtgacat tagtatctct aatggtttat ctggtggact taatggaaat
     2941 acagcttttt ctgaaacaat taattataaa caagaaagtt acagaacaac attaagtcgc
     3001 aacacaaatt ataaaaatgt tggctgggga gttgaagcac ataaaattat gaataatggt
     3061 tggggacctt atggaagaga tagcttccac ccaacatatg gtaatgaact cttcttagct
     3121 ggcagacaaa gcagtgcata cgctggccaa aacttcatag cgcaacacca aatgccatta
     3181 ttatctagaa gtaacttcaa tccagaattt ttaagcgtac tatcacacag acaagatggc
     3241 gctaaaaaat ctaaaattac agtaacttat caacgtgaaa tggatttata ccaaattcgt
     3301 tggaatggct tctactgggc aggcgcaaat tataaaaact ttaaaactag aacatttaaa
     3361 tcaacatatg aaattgattg ggaaaatcac aaagtgaaat tgttagatac aaaagaaact
     3421 gaaaacaata aatagctagt aaaacacggt cgccaacagt aattgtgacg accgtgtttt
     3481 gatttattat cttagtaata ctgccattct ttttctcaat gtgagatata aaggaatagc
     3541 tacaattaaa gtgaatatta cgcctggaat cgcgtttaac aacactaccc acacaggtaa
     3601 atttaaaata atagataata gtagcctaga taccaaactg cctaatacac ttgctaaaac
     3661 taatgatagt acatttattt tcaataaata aacaactgca atagctataa ctctaaatat
     3721 aatagagata atcacattaa ttggattaaa tacgccaaat actagtaata ataggctaga
     3781 taataatcca cctaaaaagt actttttaat tccaaagaaa gctaatatca ataatgctgc
     3841 cggtgcagat aattgaaaat ctaatcctgg tataatggac ggtattttca aaactgccaa
     3901 aatggttaaa atcgcagcaa tgacactaat ttgagtaata tcttttgatg tcatactaaa
     3961 acccctatac cgtttcataa acaacttgct tcggtgtgct ttctaaaaat gatatgtaat
     4021 gatttaaatc aatacaatcg tccacaaata ttattctgcc tccatatctc gtattaactg
     4081 gtttaatatc aaataatcga tggtaaccaa ttttagcagc ggcaaaataa cctgtcgtat
     4141 acgttaagtc atcggacacg caaagttctc ctttgacata cggatgcgca ttgatacaac
     4201 tagcaattgc taaggcatca gtcactcttt cattaagatc acctttatta tttatatctt
     4261 caaacgaaaa atgtgttgcc ctaatccccc tttgtccaaa tgaatctaaa cgtttaccag
     4321 atatagcaga tagaataata gctcctgtat aaaccgtttc atttttaata tatgtcatcc
     4381 cttgatttag cgcttgttca gtgacaccac attcttgtgt taaatgttgg agatttgctt
     4441 tatcatcctc aataatttgt aatgctttta tttgttgaat cggttccatg attttttgta
     4501 tttttatatt gagaaaatcc ggttgcccat tttcatgaaa aaaaccttta ttataaaacc
     4561 tttgtaccgt ctgttctatt ttgtcaaact cacaaatcgt ttcagcacca ctaatatgaa
     4621 catcttgatt gctagaacgc atttttatac tatacatgac gatcacctca atcttcttga
     4681 tgcaaaattt caaacaacct atctatatct tgttcagtat gaaaatacga caatgatatt
     4741 cttaacattg gcttagtcac agttggatac cttaaataac ttgtaaacac atgatgcttt
     4801 aataatgttt gatgaatgtt ctcagccgct tctatgtcat caaactcaat aaacttaatc
     4861 ggcgagtttg aactattata attaacattg agtgctttta acttttggtt aaaatattta
     4921 ctcaaactat ttaatttagt gcgtctatca tcagcattta ttaacttttc aatgtttctt
     4981 tttataaaat acaaattata aattggcaaa ctacttgagt agatgagtgg tctaccgtga
     5041 ttaattaaca tatccttcac atcatttgaa cttaaaatca cacccccgta tgcaccacat
     5101 gctttagata aactagaagt gagtatatct acaccttgat aattcgagta attctctatt
     5161 ccaaaactat gtgaaacatc gagtatcagt gttgcgttat atttatgctt taatgagact
     5221 aattgaccaa tatccacaac gtcgccattc gttgaaaata cactatcaga tatgattatt
     5281 tttggtatat tttgattagg gtatttctct aacctttttt ctaaatcagc aatatctaaa
     5341 tgcttatata tcactttttc taaaccactt aacttaatac cgtcaataat actcgcatga
     5401 ttttcttgat ctgaaaacac gacacaattt gtatttttga aaatattaaa taacgccaaa
     5461 ttagcatcat aaccactatt taagatagta catgcactat atccgagcca acctgctaac
     5521 attgtttcaa tttcttcata agctgtcgaa cttccactaa ttaatcttga acttgataag
     5581 tgataactat acttccgcat aaatctttcg aaatcatcct tatcaaacgc tatttgacct
     5641 aatcctaaat aatcattaga tgtatagttc gtacatctct tattttctac ttcaatatac
     5701 tgtctatcta tataccctac cgatttaagc gaccgatata accctttctg ttgtaataaa
     5761 tcaatttgct cttgaaactt cattcttgtt ttccttattt tcacaagtgt cataatcaat
     5821 ttcaaagcct aaatcattaa tcatatcgta gtctaattgg ttcggttgcc caccagtaat
     5881 tagataatca ccgacaaata ttgaattcgc cgcttttaat gctaatggct gtaacgaacg
     5941 taagttgacc tctcttcctc cagcaatacg aatttctttc gtaggattga ttaatcggaa
     6001 taatgctacg attcttaaac atttcattgg tgttaaatca tccatgcttc caaactttgt
     6061 gcctttgatt ggatgcaaaa aattaatcgg aatactgtcg gcatccattt cttttaaagc
     6121 aaatgccata tcaacaatat cttgattaga ttctcccata ccacaaatca cgccagaaca
     6181 tggtgatata ttattcgctt tcattagttc tatcgtatct gttctatctt tataactatg
     6241 cgttgtcacg acgttatcat ggtaattttc acttgtatta atattgtggt tatatctgtc
     6301 tacaccagct gacttaagtt tcttagcttg ttcgtcattc gttaatccta aacatgcaca
     6361 gatttttagt tgcgggtgtt gagatttaat cgttcttaca gtattactaa tatgatcaac
     6421 ttctttatcg ctcggtcctc taccactcat aacaatacaa tatgttccaa tatgattatc
     6481 atgtgccacc tttgctccat cgataatttg ttcctctgga attaaagcat atcgctgttt
     6541 ttgtttaata tctcgtgatt gtccacagta cccacaattt tcaggacata taccactttt
     6601 agcatttaaa atcatgttta attttacttt tttaccaaaa taatgttttc ttaaaatgta
     6661 cgcctcattt aataaatcta aggtatcaat attagtatcc tcataaattt tcaatacagt
     6721 ctcttttgtt aattgttccc cttgtaatat gcgtttagcc aaattcatat taacacttcc
     6781 tatctaaaat gttatgtact gcctttttaa aaatacgtac catcgttttc acttctttat
     6841 ttgacatact taacactggc acaaacgtaa tgacattttc taaatttcta atcattagcc
     6901 cgttttcttt acaattacgt acaatacgtt caactttttc aatttctaac ggcgttttac
     6961 tatctttatc tgtaactaat tcaactccaa acattaagcc tcgtcctctt acatcaccaa
     7021 catttcgatg acgcttcagc gcatgtaact gtttctcaag tgttgatgat gtcgtttcaa
     7081 tatgtgacaa taacttacgt ttttcataaa gtcttatatt ttctaatgca accgtacaaa
     7141 cgatttgatt tccggtgtat gtatgaccat ggaaaaaggt attcacacca tgcgaatcac
     7201 ttaaaaatgc attgtatatt tttttagatg tcaatgtagc tgcaagtggt aagtagccac
     7261 cagtaatcgc cttacctaaa cacataatat ccggttgaac atcttcatga ttgcatgcaa
     7321 acatctttcc agttctccca aaaccaactg ctacctcatc acaaattaat aagacatcgt
     7381 attttttgca caatttctcg acttctttca aaaagccttt aggatgaaca aacaagcctg
     7441 tcgcaccttg aatcaacggt tccaatataa accctgcgat ttcatcattt ctttcaatta
     7501 gaatttgctc tatattcgtt aaaatagctg tcatcatttc attttcagta tcgtaattac
     7561 tgcgatagaa agatgggctt tctacttgaa tattctcaaa tattaagtct ttaaatattt
     7621 tatgaaaggt cttgatacca ccaacactta ccgcaccaat cgtatcccca tgataaccgt
     7681 gatttagcgt tataaacttg tttttcttgg catatttttc tctatcaata tttttccaat
     7741 actgatatgc catctttatt gcgatttcaa cagacgcact gcctgtatca gaataaaata
     7801 cttttcttag attacttggc gtgatttcga ttaatttttc cgcaagttct attgacggaa
     7861 tatttgatga tcctagcagc gtagaatggg caattttatt gagttgcttt ttaattacct
     7921 tatttaagta tttgttatta tgaccatgca cattgaccca caacgatgca taaccatcta
     7981 aatatttatt gccattcgta tcgtaaaggt aactaccctt tcctttttca atgatgattg
     8041 cttcttcttt gctatataca cccatttgtg taaatggatg ccaaacatat tctgagtctt
     8101 tttgtttaag ttgttgtgtg taattcattt ttaactccta ttaattgttt taaaaatgct
     8161 tctgagaaat tttcatacgt ggcatgttct tcaaatgtat agactgtttt atttgttaat
     8221 tttccaatcg tcatttgatt gtctttttca atatagctgt ctgtatagcg attcattatt
     8281 aaaaaattac tcgccgatac attctgatta acataatctt ggtgaacaat ggcatcgcta
     8341 atagcaccta attttgatgg caacacactg atgacacaat ctgcacaatc attgattaga
     8401 tctttagtca tgtagaaatc atctgtacct tcatatattg gtacggcaat tcccccagca
     8461 ccctcaatta agataaaatc aaattcctta tctaaaactt ttaccttatc taatacacgc
     8521 tgcttattta gaaaaatttg atctgtcatt ttaaatgcaa ggtgtggtga tacaggttgc
     8581 ttaaaagtat aaagtgacgt tatgtcatag cttaaatcac attcattttt aaatacttct
     8641 aaatctggaa acgtcccgtc ttggcgttcc tcagtttgaa atggtttaaa aatacaaact
     8701 cgatgaccac gtgttttcaa agcatggtat aaatgctttg taacataggt tttgcctaca
     8761 tcagtattcg tacttgtaat aaaaatcctc ataaaaataa ccacctcaat ccaaatgtaa
     8821 acttttataa ttaataagtt tacatttaag gcgcatgttt ttcaagaaaa catgaattta
     8881 aacttctcag tataactata aattttggca aaattttgac cttgctattg aaatgactaa
     8941 gacttcttaa ggaattcggg cagatataaa cagcgattcg ataaaatatg tcagttaaaa
     9001 ttaataattg cattatttct taataaaaag acatcctagc tttaccatct tgtgaaacaa
     9061 tccattaatt gtaatattac gttgagcgcc ttgttcatca atgctataat tagttacata
     9121 ataaattgaa catctaaata caccaaatcc cctcactatt gccctagtga ggggatttat
     9181 tttgctgttg attatctatc acccttttta tttccaaatt ctgattccat attaaactat
     9241 tatttataat ttccagcctg ctgatttact ttttattctt ataaagtctt gatacattcc
     9301 tggttttcga attaattcat catgactacc tttttgaatt atttcacctt cattgagcac
     9361 tataatctga tctgcatttt taatagtttc aagtttatgt gcaattgtaa ttactgtttt
     9421 gcctttactt aattcattaa ttgccgtttg aatcagctgt tcattttcag ggtcaatact
     9481 tgcagttgct tcatctaaaa taattattgg tgcatctttt aatatcgccc tagcaataga
     9541 aatcctttgc ttttctccgc cagataaatt actacctttt tcatttagca ttgtttgata
     9601 tccctcaggt aatgacatga tgaagtcgtg gcaacatgct tgcttcgctg cacgaataat
     9661 ttcttctttc gttgcacctg gattgccaaa caatatgtta ttttcaatcg tatcattaaa
     9721 taaatacacc ttttgaaata ctgcactaat tttcgacatt aacgtactta atgtcatatc
     9781 tttaatatca acaccatcga tgcgaatatt tccatcatcg atatcataaa agcgcaagag
     9841 taagtgacat aatgtagatt tcccacttcc tgaaggtcca attattgctg ttgatgtttg
     9901 tgtaggtatc tcaaaattca cattctttat cacttgttta tcatcatatg aaaagttcac
     9961 gttttgaaaa gcgatatttt catttttaat cgtcaaattt tttccattct catccagttc
    10021 tggagcattt tttatctttt caatatcatc tatcgtcata tctatcattt caagtacgtg
    10081 tgcagcacta ttaatatttt caacactatc aaatatcaca aatgaaaaaa ttgaaatcat
    10141 aataagggta ggtaaatcaa tactatgatt catatataat aagcatgcaa ctaatacaat
    10201 cattattgaa acaactttta aactaagtaa atgcaataaa ttgaatggga tatattgcat
    10261 ttctattttt gtatttatac gtttactttc attgactgct tggttaaaac ttttaagact
    10321 cgtattttct ttcgcgaatg attttattac ttgaatacca cgaataacct ccaatacctt
    10381 ttccactaat tggttttgta cattatgata cgctggcgca ttttgtcgac tctttctttc
    10441 taataattga atcgcaaaaa atgatagtaa tacgccaatg catgctaata atgatacttg
    10501 ccacgaaact acaagtagag acaatatgag tactgtaatt aatatgtatc cattaacaac
    10561 aacgtccacc attttcatag caaagttttc taaaaaggtt aaatctgttg ttactattgt
    10621 tgttaactca tttgaatgat gcgaattaaa ataacctaac cttacatttt tcaatttatc
    10681 ccctatatct aaacgttctt tcgcactcat ttcataagcg atgctctcat ggcttttgct
    10741 tttgaaatat gctgtaataa atcgtccaat cactaataaa accatgataa ttacaacatt
    10801 caatatatct ttcatataaa taggtttatg agatagcaca ttattaaata tttttgcagc
    10861 taaaaagata ggtaacgcaa taaaaatagc atttaaaaat gacatgctaa atcctaaaat
    10921 cattcttgct ttatatggtc ttatccagtt taaaatttta aatgtaattt gaaacatatt
    10981 tgacctcctt ttctcattag tttaattatg tccagtattg attccccaat cttttgtatg
    11041 catgtgcgta tcccacattt tcttataatt accgtttaat tttagcaata agtgatgtga
    11101 ccctttttct aaaatttgtt gtttacctaa aacaataatt tgatctgcat gttgaatcgt
    11161 agataaccga tgtgcaataa caatcaatgt tttatcctgc gtcaacacat ttaatgcttc
    11221 ttgaattttt tgttcgttgt ctggatcaac gtaaacagtc gcttcatcta atacaatgat
    11281 aggcgcatct tttaatatca ttcttgcaat agtgactcgt tgtttttcac caccagacaa
    11341 tttatctccc actgtaccaa cattcgtatc atatccatct ggcaactttt caataaattc
    11401 atgacattgt gctaacttgg cagctttttc aactgcctca tccgtagctt ctggattgcc
    11461 aagtttaata ttttctttaa aagtaagatt taataaaaag ttatcttgtc caacaaaacc
    11521 aactaaatcg ttaagttgtt tcgattcaat atcttttata tttataccgc caatcgtaat
    11581 ttcacctgaa gtcacatccc agtatcgtga tataagcttg gcaatggttg acttaccgct
    11641 accagatgcc ccgacgatag ctgtgaaatt attttctggt actgtaaatg ataaatgctt
    11701 aaagaccaga tcgtccttat ctttgttata tgaaaatcca acattattaa aagcaatttc
    11761 ataatgttga ggcttcttaa attttgttga caatactaac tcttctagac ttaatatttg
    11821 attcacttca gttaatgcgt attgtataga ctttaaatga tttacataat tagtaaaatt
    11881 cttaatcggt gctactacac ctaaagataa tacgatgcat aggaaaaatt ccgcatagtt
    11941 taattggttg atagatatca aatacatgcc gaccggtaaa atccctaaaa atgttgaagg
    12001 taatacacta gctcctaaat tcatataccc ccatgtattt ttaaaccaat tcaaagtgtg
    12061 aatcttataa ttatctactg catctttata ttttttatat gaactttgag attgattaaa
    12121 tgttttaatc acttcaatgc cttcgataaa ctctacaatc gcgctattca tataattatt
    12181 cgatttcatt tgttcagcat atgtctcatt aaatccagac ataacttttt taaaagcgaa
    12241 aattgaaatt ggtatcgtta ctaataaggc actagccata cgccaatcaa tgagcattat
    12301 gtataaaaag atagcagctg acaaaagtaa gtttcctata acttcaggaa tcatatgtgc
    12361 taaaggtaat tctattgttt caaccttatc gacaaatata ttttttaatt cacctatttt
    12421 cttagattcc actacgccta aagggagacg cattaatttt tgagctaatt ttttacgaat
    12481 ttcagataaa atttcatatg ccgtaatatg tgatagcatc gttgacgctc caaaacaaca
    12541 cacttgtgaa atataagcga ttaaagcaat aaagatataa accataatcg aattaatcgt
    12601 a
//
LOCUS       DANJFZ010000076        12453 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.076, whole genome shotgun sequence.
ACCESSION   DANJFZ010000076 DANJFZ010000000
VERSION     DANJFZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.076"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..141
                     /locus_tag="PWN45_001849"
     CDS             <1..141
                     /locus_tag="PWN45_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623616.1"
                     /translation="SWGEGPNTEADEKSAYNNVQVGDGPQQREIGFPISTDNASWGGT
                     TK"
     gene            476..3181
                     /gene="acnA"
                     /locus_tag="PWN45_001850"
     CDS             476..3181
                     /gene="acnA"
                     /locus_tag="PWN45_001850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDL0623617.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3361..3828
                     /locus_tag="PWN45_001851"
     CDS             3361..3828
                     /locus_tag="PWN45_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0623618.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(4035..4331)
                     /locus_tag="PWN45_001852"
     CDS             complement(4035..4331)
                     /locus_tag="PWN45_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDL0623619.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4707..5315)
                     /gene="plsY"
                     /locus_tag="PWN45_001853"
     CDS             complement(4707..5315)
                     /gene="plsY"
                     /locus_tag="PWN45_001853"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL0623620.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5521..7512
                     /gene="parE"
                     /locus_tag="PWN45_001854"
     CDS             5521..7512
                     /gene="parE"
                     /locus_tag="PWN45_001854"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL0623621.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7512..9914
                     /gene="parC"
                     /locus_tag="PWN45_001855"
     CDS             7512..9914
                     /gene="parC"
                     /locus_tag="PWN45_001855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL0623622.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10164..11624
                     /locus_tag="PWN45_001856"
     CDS             10164..11624
                     /locus_tag="PWN45_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL0623623.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            12124..>12453
                     /locus_tag="PWN45_001857"
     CDS             12124..>12453
                     /locus_tag="PWN45_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0623624.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYK"
BASE COUNT         4380 a         1766 c         2363 g         3944 t
ORIGIN      
        1 agttggggtg agggccccaa cacagaagct gacgaaaagt cagcttacaa taatgtgcaa
       61 gttggggatg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
      121 tggggtggga cgacgaaata aattttgcga aaatatcatt tctgtcccac tcccatcaaa
      181 agaatgacat tgaaaaaaat attaaagttt ggtgccataa ttttctttct gctcaatgaa
      241 ataatacgtt taaaaattga attgaatcat tcgcgaaatt gataattatt ctcaattaat
      301 aaaaaatact tattacacct tacctgtatg ataagttttg cttatatact ctgattaaaa
      361 agtcaaaacc taaattaagc gttttctaag catgatgagt tgtttatcaa taaatataat
      421 aatacaattt aatcaaggca tataaatata aaaatgtatc aagggggatc attaaatggc
      481 tgcaaatttt aaagagcaat caaaaaaaca ttttgacttg aatggccaaa gttatactta
      541 ctatgattta aaagctgtag aagagcaagg tattactaaa gtttccaatt taccttattc
      601 aattcgtgtt ttgttagaat ctttacttcg tcaagaagat gattttgtaa ttacagacga
      661 tcatattaaa gctttaagtc agtttggaaa agatggaaat gaaggcgagg tgccatttaa
      721 accttctcgt gttattttac aagatttcac aggtgtacca gccgtagttg atttagcttc
      781 tttacgtaaa gcaatggatg acgttggggg agatattact aaaattaatc cagaagtacc
      841 ggtggattta gttattgacc actcagttca agtggatagc tatgcaaatc cagaagctct
      901 tgaacgtaat atgaaattag aatttgaacg taactatgaa cgttatcagt ttttaaattg
      961 ggcaacgaaa gcatttgata attacaatgc agttcctcct gcaactggaa tagttcacca
     1021 agttaactta gaatatttag caagtgttgt acatgttcgt gatgtagatg gtgaaaaaac
     1081 tgcatttcca gatacattag ttggtactga ttcacataca acaatgataa atggtattgg
     1141 cgtactagga tggggtgttg gtggtattga agctgaagct ggaatgcttg gacaaccttc
     1201 ttatttccca attccagagg ttattggtgt acgactagta aattcattac cacaaggcgc
     1261 aacagcaact gatttagcgt taagagtaac tcaagagcta cgtaaaaaag gtgttgttgg
     1321 taaatttgtg gagttctttg gtccaggtgt acaacattta ccactagcag accgtgctac
     1381 aattgcaaac atggcaccag agtatggagc aacttgcgga ttcttcccag ttgatgatga
     1441 atctcttaaa tatatgaagt taactggtag atcagacgaa catatcgcgc tagtaaaaga
     1501 atatttgaaa caaaaccata tgttctttga tgttgagaaa gaagatccta attatacaga
     1561 tgttatcgaa ttggatttat caacagttga agcatcgctt tcaggaccaa aacgtcctca
     1621 agatttaatt ttcttaagtg atatgaaatc atcatttgaa aattctgtaa cagctccagc
     1681 aggcaaccaa ggacacggtt tagataaaag tgaatttgat aagaaagctg aaattaactt
     1741 taaagatgga tcaaaagcta caatgaaaac aggtgatatt gcaatagcag caattacatc
     1801 atgtacaaat acatctaacc cttatgtaat gttaggtgca ggtttagttg ctaaaaaagc
     1861 agttgaaaaa ggcttgaaag ttcctgaata cgttaaaact tctctagcac caggatcaaa
     1921 agttgttacc ggatatttaa gagatgctgg cttacaacct tatttagatg atttaggctt
     1981 caacttggtt ggttatggat gtacaacttg tatcggtaat tcaggtcctt tattaccaga
     2041 aattgaaaaa gcgattgctg atgaggacct attagtgaca tctgtattat ctggtaaccg
     2101 taactttgaa ggtcgtatcc atcctcttgt taaagccaat tacctagctt caccacagtt
     2161 agttgttgct tatgcattag ctggaacggt tgatattgat ttacaaaatg aacctattgg
     2221 taaaggtaat gacggtgaag atgtatattt gaaagatatt tggccatcaa ttaaagaagt
     2281 ttcagatacc gttgatagtg ttgtaacacc tgaattattt attgaagaat ataataacgt
     2341 atacaataac aacgaattat ggaatgagat tgatgtaact gatcaacctc tatatgactt
     2401 tgatcctaat tcaacataca ttcaaaatcc atcattcttc caaggattat ctaaagaacc
     2461 gggtacgatt gttccattaa atggtttacg tgttatgggt aaattcggtg attctgtgac
     2521 aactgaccac atctctccag caggtgcaat tggtaaagat acgccagctg gtaaatattt
     2581 acaagatcat caagtgccta ttcgtgaatt taattcatat ggttcaagac gtggtaatca
     2641 cgaagtaatg gttcgaggta cgtttgctaa tatacgtatt aaaaaccaat tagcgccagg
     2701 tactgaaggt ggttttacaa cttattggcc aacaaatgaa gtaatgccta tctttgatgc
     2761 tgcaatgaaa tataaagaag atggtacagg tttagttgta ttagctggta acgattatgg
     2821 tatgggttca tctcgtgact gggcagcaaa aggtacaaac ttattaggtg ttaaaacagt
     2881 tattgcacaa agttatgaac gtatccatcg ttcaaattta gttatgatgg gtgtattacc
     2941 attagagttt aaaaaaggtg aatcagctga ttctcttggt ctagatggta cagaagaaat
     3001 ttctgttaat attgatgaaa atgttcaacc acatgactac gtcaaagtta ctgctaagaa
     3061 gcaagatggt gatttggtag aatttgacgc tatggttcgt tttgactcac ttgttgaaat
     3121 ggattactat cgtcacggtg gaattttaca aatggtttta agaaataaat tagcgcaata
     3181 aaaaatagat atcacagtaa aattttaatc ggtatttgag gcactttgat agtgctcaaa
     3241 aatacttcta taaactttat gatataatag gtcggttcaa aacctttgtg ttcttggccg
     3301 gcctcttctt gtattttatg ttagaatgag atataaagtt aaactgaaag aaggcttaaa
     3361 atgatatata gtattacaga aatagaagcg cgttatgctg aaactgataa gatgggtgta
     3421 atttatcacg ggaattatgc aacttggttt gaagttgcgc ggttggatta tatatcgaag
     3481 ttaggtttta gttatgctga tatggaaaaa caaggaatca tttcacctgt gactgacctc
     3541 aatgtcaatt ataaaaagtc tattttttat ccagaaaaag ttaaagttaa aacttgggtt
     3601 gaaaaatatt cgagattacg ttcagtgtat aaatatgaga tttttaatga aaaaggtgaa
     3661 cttgcaacta caggttccac agaattgatt tgcattaaag aagatacttt taagcctata
     3721 cggttggatc gttatttccc agattggcat gaagcttata gtaaagtgca aacgctcaat
     3781 aatgaaggga aaacagtaga gataatggat ggtattgatt ctttataagt tactgtaaat
     3841 gtttggagag gcaatattaa caattaatct ataaaaaata gccaatacgt taacattaat
     3901 gtaattagcg tgaagataag ctaatatata gcaaaaacga acttgttcat acaacatcag
     3961 atgtgctaaa ccaaagtaca acttccgagg ttcaggacac atcagaatgt gtaaacaagt
     4021 tcgttttatt ttagttattt tgtggaataa gaaatttcat cttcgtgatc aactacattt
     4081 acaataatgt ggtcatcttc aaagtaccac aaatcttttt ccgctacgac aacatttaaa
     4141 tcgtcatatt gttgttcata gccaatatca acatcttcct ttggttcaac tgtaaaagca
     4201 ggactaaatc cttgcttgag ttggaattcg ccaccatatc ttacaaaaaa cacgagcact
     4261 ttattatttt caggcaactc aagttcattt ttaaaccaag ttactgctgc atcagtaagt
     4321 tctatttgca taacaatgac ctcctatatt tattacagaa tgggagtggg ccaacttgta
     4381 ttatttgtgg aatttcttat cgaaattctc tgtgttggag cccaccccaa cctgctcatt
     4441 attgtaagct gaatttccat aagcttctat gttgggaccc ccgaccagaa ttgaaaaaac
     4501 ctagttataa gcgcattttg ttcggttaac tacagctaat ataacattgt ggagcctatg
     4561 acattgattt atgtctcggt ctcaaatgtt cttgtaaaaa aactaagtat agttgaattt
     4621 taagttaaaa aaattgtttt tgcaaaaata aaataatcca tcaactgcaa aaaaatatgc
     4681 aatgatggac taatgtcaaa aaattgttac atccatttta ttttaggttc ttcgcctcta
     4741 aaaatccttg cgatattaga gcgatgtcta attatcaata tgattgaaac taagaaacta
     4801 acgactaata aaatatagtc ttgaatgata agcgagccaa tcacacagca aattgctgca
     4861 acgatacttg ctaaagaaac atatttaaaa atcttcaata caataaagaa gataattgca
     4921 agtattagta aaagtatcgg attgactccc aagacgacac ctgcactagt tgcaacagct
     4981 ttgccacctt ggaattttaa ataaacagga taaacgtgtc caagtatagc gaataagcca
     5041 acaattaaac catttgtaaa aaaagtacta atagggccat ctgcgtgaac ttgtaaccat
     5101 aaagggaaga aaacagttat gaaccctttg aaaatatcta gaaatgttac caagaatcct
     5161 gcaggacgac ctaatactct aaagctatta gtagcgccag tattaccact accaaattgt
     5221 ctaatatctt ttttgaaaaa taattttcca attacgaatc cacttgggaa agcgccgata
     5281 agataactta gtagtaacat gacgattatc atcataaata ttacacatcc tttaatatct
     5341 taggactatt ttatcatatt catttaaatt acggctaaaa attttaaaaa cggggattaa
     5401 tatatggaat taagctatga aagttaattg atacttgcat tttacgctga tttatataag
     5461 aataactatt gtatagtttt aaaaacgaac gtacgtttgc aggaggcgaa atcattggca
     5521 atgaataaac aaaataatta ttcagatgat tcaatacagg ttttagaggg gttagaagca
     5581 gttcgtaaaa gacctggtat gtatattgga tcaactgata aacggggatt acatcatcta
     5641 gtatatgaaa ttgtcgataa ctccgtcgat gaagtattga atggttacgg taacgaaata
     5701 gatgtaacaa ttaataaaga tggtagtatt tctatagaag ataatggacg tggtatgcca
     5761 acaggtatac ataaatcagg taaaccgaca gtcgaagtta tctttactgt tttacatgca
     5821 ggaggtaaat ttggacaagg cggctataaa acttcaggtg gtcttcacgg tgttggtgct
     5881 tcagttgtaa atgcattgag tgaatggctt gaagttgaaa tccatcgaga tggtaatata
     5941 tatcatcaaa gttttaaaaa cggtggttcg ccatcttctg gtttagtgaa aaaaggtaaa
     6001 actaagaaaa caggtaccaa agtaacattt aaacctgatg acacaatttt taaagcatct
     6061 acatcattta attttgatgt tttaagtgaa cgactacaag agtctgcgtt cttattgaaa
     6121 aatttaaaaa taacgcttaa tgatttacgc agtggtaaag agcgtcaaga gcattaccat
     6181 tatgaagaag gaatcaaaga gtttgttagt tatgtcaatg aaggaaaaga agttttgcat
     6241 gacgtggcta cattttcagg tgaagcaaat ggtatagagg tagacgtagc tttccaatat
     6301 aatgatcaat attcagaaag tattttaagt tttgtaaata atgtacgtac taaagatggt
     6361 ggtacacatg aagttggttt taaaacagca atgacacgtg tatttaatga ttatgcacgt
     6421 cgtattaatg aacttaaaac aaaagataaa aacttagatg gtaatgatat tcgtgaaggt
     6481 ttaacagctg ttgtgtctgt tcgtattcca gaagaattat tgcaatttga aggacaaacg
     6541 aaatctaaat tgggtacttc tgaagctaga agtgctgttg attcagttgt tgcagacaaa
     6601 ttgccattct atttagaaga aaaaggacaa ttgtctaaat cacttgtgaa aaaagcgatt
     6661 aaagcacaac aagcaaggga agctgcacgt aaagctcgtg aagatgctcg ttcaggtaag
     6721 aaaaacaagc gtaaagacac tttgctatct ggtaaattaa cacctgcaca aagtaaaaac
     6781 actgaaaaaa atgaattgta tttagtcgaa ggtgattctg cgggaggttc agcaaaactt
     6841 ggacgagacc gcaaattcca agcgatatta ccattacgtg gtaaggtaat taatacagag
     6901 aaagcacgtc tagaagatat ttttaaaaat gaagaaatta atacaattat ccacacaatc
     6961 ggggcaggcg ttggtactga ctttaaaatt gaagatagta attataatcg tgtaattatt
     7021 atgactgatg ctgatactga tggtgcgcat attcaagtgc tattgttaac attcttcttc
     7081 aaatatatga aaccgcttgt tcaagcaggt cgtgtattta ttgctttacc tccactttat
     7141 aaattggaaa aaggtaaagg caaaacaaag cgagttgaat acgcttggac agacgaagag
     7201 cttaataaat tgcaaaaaga acttggtaaa ggcttcacgt tacaacgtta caaaggtttg
     7261 ggtgaaatga accctgaaca attatgggaa acgacgatga acccagaaac acgaacttta
     7321 attcgtgtac aagttgaaga tgaagtgcgt tcatctaaac gtgtaacaac attaatgggt
     7381 gacaaagtac aacctagacg tgaatggatt gaaaagcatg ttgagtttgg tatgcaagag
     7441 gaccaaagta ttttagataa ttctgaagta caagtgcttg aaaatgatca atttgatgag
     7501 gaggaaatct agtgagtgaa ataattcaag atttatcact tgaagatgtt ttaggtgatc
     7561 gctttggaag atatagtaaa tatattattc aagagcgtgc attgccagat gttcgtgatg
     7621 gtttaaaacc agtacaacgt cgtattttat atgcaatgta ttcaagtggt aatacacacg
     7681 ataaaaattt ccgtaaaagt gcgaaaacag tcggtgatgt tattggtcaa tatcatccac
     7741 atggagacta ctcagtgtac gaagcaatgg tccgtttaag tcaagactgg aagttacgac
     7801 atgtcttaat agaaatgcat ggtaataatg gtagtatcga taatgatccg ccagcggcaa
     7861 tgcgttacac tgaagctaag ttaagcttac tagctgaaga gttattacgt gatattaata
     7921 aagagacagt ttctttcatt ccaaactatg atgatacgac actcgaacca atggtattgc
     7981 catcaagatt tcctaactta ctagtgaatg gttctacagg tatatctgca ggttacgcga
     8041 cagatatacc accacataat ttagctgaag tgattcaagc aacacttaaa tatattgata
     8101 atccggatat tacagtcaat caattaatga aatatattaa aggtcctgat tttccaactg
     8161 gtggtattat tcaaggtatt gatggtatta aaaaagctta tgaatcaggt aaaggtagaa
     8221 ttatagttcg ttctaaagtt gaagaagaaa ctttacgcaa tggacgtaaa cagttaatta
     8281 ttactgaaat tccatatgaa gtgaacaaaa gtagcttagt aaaacgtatc gatgaattac
     8341 gtgctgacaa aaaagtcgat ggtatcgttg aagtacgtga tgaaactgat agaactggtt
     8401 tacgaatagc aattgaattg aaaaaagatg tgaacagtga atcaatcaaa aattatcttt
     8461 ataaaaactc tgatttacag atttcatata atttcaacat ggtcgctatt agtgatggtc
     8521 gtccaaaatt gatgggtatt cgtcaaatta tagatagtta tttgaatcac caaattgagg
     8581 ttgttgcaaa tagaacgaag tttgaattag ataatgcaga aaaacgtatg catatcgttg
     8641 aaggtttgat taaagcgttg tcaattttag ataaagtaat cgaattgatt cgtagctcta
     8701 aaaacaagcg tgacgctaaa gaaaacctta tcgaagtata cgagttcaca gaagaacagg
     8761 ctgaagcaat tgtaatgtta cagttatatc gtttaacaaa tactgacata gttgcgcttg
     8821 aaggtgaaca taaagaactt gaagcattaa tcaaacaatt acgtcatatt cttgataacc
     8881 atgatgcatt attgaatgtc ataaaagaag aattgaatga aattaaaaag aaattcaaat
     8941 ctgaacgact gtctttaatt gaagcagaaa ttgaagaaat taaaattgac aaagaagtta
     9001 tggtgcctag tgaagaagtt attttaagta tgacacgtca tggatatatt aaacgtactt
     9061 ctattcgtag ctttaatgct agcggtgttg aagatattgg tttaaaagat ggtgacagtt
     9121 tacttaaaca tcaagaagta aatacgcaag ataccgtact agtatttaca aataaaggtc
     9181 gttatctatt tataccggtt cataaattag cagatattcg ttggaaagaa ttgggacaac
     9241 atgtatcaca aatagttcct atcgaagaag atgaagtggt tattaatgtc tttaatgaaa
     9301 aggactttaa tacagatgca ttttatgttt ttgcgactca aaatggcatg attaagaaaa
     9361 gtacagtgcc tctatttaaa acaacgcgtt ttaataaacc tttaattgct actaaagtta
     9421 aagaaaatga tgatttgatt agtgttatgc gctttgaaaa agatcaatta attaccgtca
     9481 ttactaataa aggtatgtca ttaacgtata atacaagtga actatcagat accggattaa
     9541 gggcagctgg tgttaaatca ataaatctta aagctgaaga tttcgttgtt atgacagaag
     9601 gtgtttctga aaatgatact atattgatgg ccacacaacg cggctcgtta aaacgtatta
     9661 gttttaaaat cttacaagtt gctaaaagag cacaacgtgg aataacttta ttaaaagaat
     9721 taaagaaaaa tccacatcgt attgtagctg cacatgtagt gacaggtgaa catagtcaat
     9781 atacattata ttcaaaatca aatgaagaac atggtttaat taatgatatt cataaatctg
     9841 aacaatatac aaatggctca ttcattgtag atacagatga ttttggtgaa gtaatagaca
     9901 tgtatattag ctaaaaacta tatgcaatca cgaaattaaa tgataaaata cagtaatgtt
     9961 aaattttgac taaattcaag ggatttatat taaatgctga ccaagtactt atcgttaaat
    10021 tagcgatata ctttgaccaa actaatataa atctcttgta ttgtataaga aatgatgatg
    10081 tatctctata tttactgaag gttcaattcg gtctaatttt atagaaggta catgattcat
    10141 gtagaggatg cgaggggttt attttgaaag atttcgatag tttaatacct ggatggttta
    10201 aagaatttgt ccatgttggt accgatttaa tatggtctca atatttaatt ggtctattat
    10261 tgacagctgg attcttcttt acgattagtt ctaaattcgt tcaattacga atgttacctg
    10321 aaatgtttag agctttagta gaacgtccag aaactttaga agatggtaag aagggtattt
    10381 cgccattcca agcatttgcg attagtgctg gttcgagagt tggtactggt aatattgctg
    10441 gtgttgcgac tgcgattgtt ttaggcggtc caggtgcagt attttggatg tgggttattg
    10501 catttatagg tgcagcgagt gcatttatag aagcgacttt ggctcaggtt tataaagtac
    10561 atgataaaga tggtggattc cgtggtggtc cagcttacta tattactaaa ggtttaaatc
    10621 aaaaatggct aggtatcgta tttgcgattt taattacaat tacatttgca tttgtattta
    10681 acacagtgca atctaataca attgcggagt cgttaaatac gcaatataat attagtccag
    10741 taatcacagg tattatttta gcaatcgtaa cagctattat tatatttggt ggtgtacgta
    10801 gtattgctac gttatcttcg ttaattgtac cgattatggc tatcatttac attggtatgg
    10861 ttttagtaat attgctattt aatttagatc aaattgttcc tatgataggt acgattatta
    10921 aaagtgcatt tggtatcgaa caagtaactg gtggcgctgt aggtgctgcg gttcttcaag
    10981 gtatcaaacg tggtttattc tctaacgaag ctggtatggg ttctgcgccg aatgcagcgg
    11041 caactgctgc cgtaccacac cctgttaagc aaggtttaat ccaatcatta ggtgtgttct
    11101 ttgatacaat gttggtttgt acagcaactg caatcatgat tttactatat tcaggactga
    11161 aatttggtga taacgcacct caaggtgttg cagttactca atcagcactt aatgagcatt
    11221 taggttctgc tggaggtatt ttcttaacaa tagcagttac actgtttgca ttttcatctg
    11281 ttgtaggtaa ttactattac ggtcaatcta atattgaatt tttatcaaca aaccgtgtaa
    11341 tattatttat ctttagatgt cttgttgtag tacttgtctt tgtcggtgca gttgtaaaaa
    11401 cagaaacagt atggaatacg gcagacttat ttatgggctt aatggcaatt gtaaacatta
    11461 tttccattat aggactgtcc aatgtagctt ttgcattgat gaaagattat caaaagcaga
    11521 aaaaagaagg caagaaccct gtctttaaac ctgaaaactt agaaattaac ttatttggaa
    11581 ttagtgcttg gggcgctaac aaatataaga actctgataa ataaatattt ttaatgtaac
    11641 gattcattat ataaaaatca aataaaccct gaggttttca aattaaagcg aaaatctcag
    11701 ggttttaaat ttgtactcaa ttatcatctg tatcattaca ctagttaatc taagatttga
    11761 ttatatatta atatagtaag gttgagattt atttcctcca ttggtgtgca aagactatac
    11821 cattgataag acatattgtt ggatagatga atataaattt gttttagctt tgcacaattt
    11881 aatagtacgt tttagtttgt attgagtatt aattcagata atatataaat aaatagaatt
    11941 atttgtaaat atgggagtca gattttttta ttatagttat tccactttga gagacatcga
    12001 aattaaatta agatatatca tataggatag gcgtgtaata gaacacaagt atttaaatta
    12061 ttatactttc aagttagaca tttccgatat ataatcttaa taatagaaaa ataggtgatg
    12121 ataatgggag aatatattgt tactaaaaca ttgaacaaca atgtcgtagt atgtactaat
    12181 aatgatcaag aagttatttt aatcggtaaa ggtattggtt ttaacaaaaa agagggaatg
    12241 gcgttaaacg accaaactat tacaatagag aaaatttata aattagaaag tgagcaacaa
    12301 aaagcacatt ataaaagttt agttgaaatc gctgatgata atgtattaca agtaattatt
    12361 gattcgttga attttatttc taatactgcg atgaatgttg attcaaaaca acttgtagtt
    12421 tcattaacgg atcatattat atttgcttat aaa
//
LOCUS       DANJFZ010000077        12142 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.077, whole genome shotgun sequence.
ACCESSION   DANJFZ010000077 DANJFZ010000000
VERSION     DANJFZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.077"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(581..1888)
                     /locus_tag="PWN45_001858"
     CDS             complement(581..1888)
                     /locus_tag="PWN45_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL0623625.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            2049..2960
                     /locus_tag="PWN45_001859"
     CDS             2049..2960
                     /locus_tag="PWN45_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0623626.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            3022..3867
                     /locus_tag="PWN45_001860"
     CDS             3022..3867
                     /locus_tag="PWN45_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0623627.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(4239..5462)
                     /locus_tag="PWN45_001861"
     CDS             complement(4239..5462)
                     /locus_tag="PWN45_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL0623628.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            5897..6952
                     /gene="lukH"
                     /locus_tag="PWN45_001862"
     CDS             5897..6952
                     /gene="lukH"
                     /locus_tag="PWN45_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDL0623629.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            6974..7990
                     /gene="lukG"
                     /locus_tag="PWN45_001863"
     CDS             6974..7990
                     /gene="lukG"
                     /locus_tag="PWN45_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDL0623630.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(8228..9058)
                     /gene="sph"
                     /locus_tag="PWN45_001864"
                     /pseudo
     CDS             complement(8228..9058)
                     /gene="sph"
                     /locus_tag="PWN45_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(9109..10146)
                     /locus_tag="PWN45_001865"
     CDS             complement(9109..10146)
                     /locus_tag="PWN45_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0623631.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            complement(10337..11050)
                     /locus_tag="PWN45_001866"
     CDS             complement(10337..11050)
                     /locus_tag="PWN45_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0623632.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            complement(11128..11310)
                     /locus_tag="PWN45_001867"
     CDS             complement(11128..11310)
                     /locus_tag="PWN45_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623633.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(11514..11855)
                     /locus_tag="PWN45_001868"
     CDS             complement(11514..11855)
                     /locus_tag="PWN45_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623634.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            complement(11861..>12142)
                     /locus_tag="PWN45_001869"
     CDS             complement(11861..>12142)
                     /locus_tag="PWN45_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0623635.1"
                     /translation="ESVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKT
                     DILVEGVQDDKYKDVNGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
BASE COUNT         4319 a         1708 c         1819 g         4296 t
ORIGIN      
        1 attaaagtaa gtttttatta gtgcataatg ccgggaatga tgtaaaactg tatatagttt
       61 tgactaatta gaaaattaca ttatgaacga gtatgatgta ttctaagtac aaattagaat
      121 gcacgcaaat atattttaat tacgagacgt tttccatttg gtgactagac ttatgggagg
      181 cgtctttttt atgtgctaat ttgtaattga gattcataat ggtatttaaa tcattatggt
      241 gcgggagtta tggtattgcc ataatatgag agtgcatcca cttctataag taatgcatat
      301 tgcgagtgca ggaatgaatg agtgcttgat taaaatcctt atgggtggtt gacataatta
      361 aaagaaacca catttaaaat ttcttaatca caagcggtta actaggtata gtttagtttt
      421 gagtaaatct ttttaaaggt gtacttgtgc attttactta attaaagaca taagacattt
      481 aatcggccta aaataaataa aaaactacct gtttaggtag tttttttaat gtaatagatt
      541 aaaacactag ttcatttctt attaaagatg gatagttatt ttatagataa atttgtcctt
      601 tagtgtagcg gtaattttta ggactttttg gtggtataaa tgttcttaat aaagttaata
      661 gtcctacttt accgcaaagc ataacgaata taataattat tttagtaata ccatgatatt
      721 ctgtggtaag gttcatactt aaccctactg ttccgaatgc agaaaccact tcgaataata
      781 acttgattaa tgatatgttc ggattaatta tcgataatat aaaagtaatg atgctgataa
      841 atagaaatga gatattaatg gtaacaatag atagttttat atgtttgtca gatatttctt
      901 tattgaatac tgaaacatta ttttctttac gtatataatt tagtacaaaa ataaacgcaa
      961 ctgcaaaagt agttatttta attcctccag ctgcactgag aggggcacca ccaataaaca
     1021 taagtagcat taacattaat gcggtagatt tgttaatgct tgctatatct atactgttaa
     1081 aacccgctgt tcgtgttgtt actgattgga aaaaagaatt tccgattttt tcaactagtc
     1141 ccatatgttg catagtatta aactgttcta ataaaaagaa tgtaatagct cctataatta
     1201 ttaggatact agttgtagtt aagactaatt tagaatgtaa agataattta ctcaattttt
     1261 tacaattaat aaagtctatc acgacaaaat gtccaatacc tccaaatatt atgagtattg
     1321 agattgtaat aatgacaatt ggatcactag aataatctat taagttattc ttaaaaaggg
     1381 caaatccagc attattaaaa gctgatactg atgtgaataa gcttaaaaat aaacctttgc
     1441 ctataccaaa ttttggtata aaagataaac acaaacaaat cataccaatt aattcagtga
     1501 ctaaactata aatagccaag tgtttaatta gcttaataac accaccaggt tcgtcaatat
     1561 tccatgtaac cataatcaag aatctatttt tcattgatat ctttctattt aaaaatacta
     1621 gtgtcaatag ggttacggtc acgataccca gaccacctat ttgaattaat aatagtatta
     1681 ctatctcacc aagtatatta aactgtgatc ctatatcaac tggggacaag ccagtaactg
     1741 taaatgcact tgaagctata aatagggcat ctaaaaaaga tattggcttt ttaccagtaa
     1801 aaggtaaata caataaaagt gcacctatga tagttgtaga gaagaaaagc attaaataaa
     1861 aatataaagg tttgtggact ttgttcattt taatttcata ctcctttata ttataaatta
     1921 ataattagat aatatcataa atgaaaatag aaatgttact gatgtgtatt actaacaggc
     1981 gttgactaat tcttttaata ttttataatt gaaaatgaat atcaattgaa aaacaaaagg
     2041 atgatattat gaaaagatta attggaattt tattatgtaa tttatttatt ttgactgcgt
     2101 gttcagcatc tgtcgataaa acaagtaact cgacaaagac tacagactat aaaattgaaa
     2161 atggtgaaac actgaaagta ccagagaaac ctaaaagagt tgctgtatta actggatttt
     2221 atgttggtga ttttataaag ttaggaatca aaccaattgc tgtttcagat ataactaaag
     2281 attcttcaat tttaaaacct tatttaaaag gggttgatta tattggagaa aatgatgttg
     2341 aaagagttgc taaagcaaaa ccagatttaa ttgttgtaga tgctatggat aaaaatataa
     2401 aaaaatacca aaaaatagcg ccaacaatcc catatacata caataaatac aatcataaag
     2461 aaatattaaa agaaataggc aagttgacta ataatgaaga taaagctaaa aaatggattg
     2521 aagagtggga cgataaaact agaaaagata aaaaagaaat tcaaagtaaa attggtcaag
     2581 caacagcatc tgtgtttgaa ccagatgaaa agcaaatata catatataac tctacatggg
     2641 gtcgtggttt ggatattgtt catgatgcat tcggtatgcc aatgacaaag caatataaag
     2701 ataaattaca agaagataaa aaaggctatg cttccatttc aaaagaaaat attagtaaat
     2761 atgctggtga ttatatattt ttaagtaaac cttcgtacgg aaaatttgat tttgaaaaaa
     2821 cacatacatg gcagaatatt gaagctgtaa aaaaaggaca tgtaatttca tataaagcag
     2881 aagattattg gttcacagat cctattacat tagaacattt gagaagtaaa ttaaaaaaag
     2941 aaattttaaa taaaaaataa tagaaataag ttgtaaaaat tttcttatgc attggtacta
     3001 atgtttttaa ggagtgatta aatgaagcaa ctggttggaa ttcccgaatc aatgttaatt
     3061 cctttgatag ctcgagcaaa agagtacgaa aacgaaaaac caataataaa agacgcacta
     3121 tcaaaaaaaa tatttgatgg tttagatgat atgtacaaaa atgttacatg tgatgacatg
     3181 tctcaaattg gaattagtat acgtactgtg ataatagatt gtgttactaa gaggcttatc
     3241 aaggataata aagatttaat cgtggtcaat ataggttgtg gcttagatac aaggtttcaa
     3301 agatttaata aagaaaaaat atcatggata gatttagatg taccagaatc aatagaaata
     3361 cgaaaaacat tttttaaaga aagtaatagt tataagatga tatctaaatc tatgctagat
     3421 tacagttgga ttgatgatgt caaaaattat aaatttttta atagtaagtc aaatatattg
     3481 tttattattg aaggtgtatt gatgtatttt gatgagagtg taatgactca attattggac
     3541 actattatca aaaagatggg agatcataat ttgacatttg cgattgaatt ttgctcaaaa
     3601 acaattgcga ataatacaaa gagacatcaa tcggtatcaa agttatcctc acaacctgtt
     3661 tttaaatatg gttacaatga tttaaaaaaa ttgaatgaaa ttttaccaaa tacaataaga
     3721 gtaatacatg aatataatta ttttgactat tataagaatc gatggggctt atttggatat
     3781 tgtagattta taccttattt aaaaaaaagg ttaaacaata aaatagtact tatgaaatat
     3841 aaagttccaa agagacagag acattaagtt cttagacaat gtaaaaaagc tgatttctat
     3901 aaattatttg atagaaatca gcttttttca aatgtatttg ataatttaca gctcgttgag
     3961 ctgctgtttt ctagggattt atgtcccatt ccttttacga gaaggttttt acgagtttta
     4021 tgtaactatt agaagctatc tttttatatg accattagtc aatatatgta tgacaaatca
     4081 gcacaataat ttcagtttta catcacttaa tcaatgaaaa tgtgcttgtt gatatttgaa
     4141 tatttacatt caaacaatat tcatcgctat ttctatcaac ttaaaattcg caataaaaaa
     4201 ctgccagcac gcgaataggg cagctgacag catcgttctt atttttcttt taaatattga
     4261 atggaagcct ctttaaaaat atcaatatat ttcagataca tatctttttc aatatattca
     4321 tcgatttgat gtgccattaa tggattacct ggtccaaaaa tggctaaatc aacattgtcc
     4381 ttattatctc ctaagaaact agaggcatct gttgcaccta caagcgctga aacaaatatt
     4441 tcgtcttgtt ctacataact agaagctaca tctttaatcg tagtaattaa tttgctattt
     4501 ttatcgcttg ttacaggtcg gtgattgctt ggaatatcga gtgaaagctt attgctatcc
     4561 acatcattaa tgatattttg gaaaaacgat tctataaagt cgttatcata ctcaggaact
     4621 ggtcttacgt taaattcaag tgaagcttca tctggtacag agttaaattg tttgccgcca
     4681 tttataatcg aacatacagc tgtaagacca gatgcataat ttgcatcctc ttcagaaatt
     4741 tcttttccaa tcaatgattt gaacataggc gcaacatcta attcatgttt agtatcttgt
     4801 tgtttaagct ctgaatattt ttctttaaat agattataaa attcaagcag tgtatcaatt
     4861 gcattgtcac caataaatgg aactgagcta tggacagctt taccagttgc agttacttta
     4921 catgacatag accccttatg tgcataataa attccagatc cagttggttc agcaataatt
     4981 aagctatcga catcgtctaa atagccttta tcagctaata atttggcacc ttcttgttct
     5041 ttctcttcgc cagcagtagc cagtaatcta atcgttccat gaggcaattc attttgttct
     5101 tttaattcga ttagagatac gaccaaagcc attaaaccgc ctttcatatc tgtagtgcct
     5161 cggccatata atttgccatc tttttctgtc agttgaaaag ggggatatga ccaattatct
     5221 tgatttcctg catcaacaac atccatatga ccactcaatg cgagtatagg tgagccgtta
     5281 ccgatttctg caacgatatt ggcgcggtgt tcattaactt tcaaaatttc agatttaata
     5341 tcgtacttgt cgaataaatc tgttaaataa ttacaaacgt ctatttcatt attattttca
     5401 gtttgtagtt caacaatatc tgctagtaat tgaatttttt ctttttcact aaaagttgtc
     5461 attaagctca cacctttcaa agtagtatat atatttatat aaacataatt tttattctta
     5521 aacataattt aaaatgcaga aagtagtgtg ttatttgatt tcgttctatg aaattaactg
     5581 tctagtatca acgatcttat taacgctgat atcggatatg cttaatttta tagtaaattg
     5641 tatgagaaag atgcaggata ttatttagct aataattata tataaatttc aaatcgatgg
     5701 ttattaacta ctaaataaaa atatttgcta aattaatgac tttgtacaca cattattttt
     5761 aaagaggata aagtatttaa taatattaac aaaatcattt aataaatagt taaatatata
     5821 ttctctgttt ttgtgatatt attcacatgt cgatacatat caacaaatat caatcatacg
     5881 aaagaaggtt ataacaatga aaaataaaaa acgtgtttta atagcgtcat cattatcatg
     5941 tgcaatttta ttgttatcag cagcaacgac tcaagcaaat tcagctcata aagactctca
     6001 agaccaaaat aagaaagaac atgttgataa gtctcaacaa aaagacaaac gtaatgttac
     6061 taataaagat aaaaattcaa cagcaccgga tgatattggg aaaaacggta aaatcacaaa
     6121 acgaactgaa acagtatatg atgagaaaac aaatatactc caaaatttac aattcgactt
     6181 tatcgatgat ccaacttatg acaagaatgt attacttgtt aaaaaacaag gctcaattca
     6241 ttcaaattta aagtttgaat ctcataaaga agaaaaaaat tcaaattggt taaagtatcc
     6301 aagtgagtac catgtagatt ttcaagtaaa aagaaatcgt aaaactgaaa tattagacca
     6361 attgccgaaa aataaaattt caactgcaaa agtagacagt acattttcat atagctcagg
     6421 tggtaaattc gattcaacaa aaggtattgg acgaacttca tcaaatagct actccaaaac
     6481 gattagttat aatcagcaaa attatgacac aattgccagc ggtaaaaata ataactggca
     6541 tgtacactgg tcagttattg cgaatgactt gaagtatggt ggagaagtga aaaatagaaa
     6601 tgatgaatta ttattctata gaaatacgag aattgctact gtagaaaacc ctgaactaag
     6661 ctttgcttca aaatatagat acccagcatt agtaagaagt ggctttaatc cagaattttt
     6721 aacttattta tctaatgaaa agtcaaatga gaaaacgcaa tttgaagtaa catacacacg
     6781 aaatcaagat attttgaaaa acagacctgg aatacattat gcacctccaa ttttagaaaa
     6841 aaataaagat ggtcaaagat taattgtcac ttatgaagtt gattggaaaa ataaaacagt
     6901 taaagtcgtt gataaatatt ctgatgacaa taaaccttat aaagaaggat aatattgaaa
     6961 gggcggatta ctaatgatta aacaactatg taaaaatatc acaatttgta cgttagcact
     7021 atcgactact ttcactgtat taccagctac ttcatttgca aagattaatt ctgaaatcaa
     7081 acaagtttct gagaagaatc ttgatggtga tactaaaatg tatacacgta cagctacaac
     7141 aagtgatagt caaaaaaata ttactcaaag cttacaattt aatttcttaa ctgaacctaa
     7201 ttatgataaa gaaacagtat ttattaaagc aaaaggtaca attggtagtg gtttgagaat
     7261 tttagaccca aatggttatt ggaatagtac attaagatgg cctggatctt attcagtttc
     7321 aattcaaaat gttgatgaca acaacaatac aaatgtgact gactttgcac caaaaaatca
     7381 ggatgaatca agagaagtta aatatacgta tggttataaa acaggtggag atttttcgat
     7441 taatcgtgga ggcttaactg gaaatattac aaaagagagt aattattcag agacgattag
     7501 ttatcaacaa ccatcatatc gtacattact tgatcaatct acgtcacata aaggtgtagg
     7561 ttggaaagta gaagcacatt tgataaataa tatgggacat gaccatacga gacaattaac
     7621 taatgatagt gataatagaa ctaaaagtga aattttttct ttaacacgaa atggaaattt
     7681 atgggcgaaa gataatttca cacctaaaga caaaatgcct gtaactgtgt ctgaagggtt
     7741 taatccagaa tttttagctg ttatgtcaca tgataaaaaa gacaaaggta aatcacaatt
     7801 tgttgttcat tataaaagat caatggatga gtttaaaata gattggaatc gccatggttt
     7861 ctggggctat tggtctggtg aaaaccatgt agataaaaaa gaagaaaaat tatcagcatt
     7921 atatgaagtt gattggaaga cacataatgt gaagtttgta aaagtactta atgataatga
     7981 aaagaaataa gtaataaaag ttgcctgcta catagaatgt agtaggtaac ttttatttat
     8041 atttgaatag atagatttat tatgacgtgc agtgtatgaa tcttgtttga gtgtagagta
     8101 aagacttgta ttaatgaaaa attaaagttg ttaagatgat tttattaaaa tgtaagtcaa
     8161 ttcaaatttt aacaatataa ctcgcttcgt ccttttagaa cgaagcaagt tattagttag
     8221 ttgagcacta tttactatag gctttgattg ggtaatgatc tgaaaaatca ttgtaaacgt
     8281 agtaatatgg gaacgcatat acatcccatg gcttaggttt ttcagtcaca acttcattga
     8341 ctaattgttt tggttgttta tgatctttat ctgtaaatat atagtctaaa tgttctggtt
     8401 taccattagg gtaattatat ttcgcaattg aatttgattg agggtcccat gtgctattat
     8461 gacctgcata tagaacatca tttacattca agtttttaag catatctttg aactctggag
     8521 tgcctttatt gacattaagg tcgccaccta tatataccgt ttcatcttta gggatatttt
     8581 tctttttaac aaagtcactg atttctttca tttgttcagc tctaattttt cgatcatgtc
     8641 cagcaccaca acgtgaatct tcagattgtg tatgtgtacc gataacgtga acgttcttac
     8701 catttttctc tatttttgta taaacaaagc ctttgttgct atcattatcg aatccacaac
     8761 cgcttttgaa aacatgctgg attttttctt taataggata tttacttaca atcgctacgc
     8821 caccatcttc tgcaacagtt gatgagtagc taccttcagt tttgtcccac cctgattgag
     8881 aacggccgag tacaggtgtt tgataaggat attctttttt cacattactt aataatttgt
     8941 ctgatgcacc attatcaaat gcttcattga atattacgac atcattattt ttaatataag
     9001 aagattgtcc gattaaatca gcgcgtttat attgccccca gtttggatac atagaaacct
     9061 tgtaacaaca gtatttattg ggtttggagt ccctaatggg tccctaaatt acatactttc
     9121 taaaatttta gttgtttttt tgtcctcttc attaaatttt tcttctaaca aatgagaata
     9181 cacggatgta gttattgcta tatttttatg acctaatctt ttagaaatgt aatgtataga
     9241 tacacctttt gctagtaaat aagaacaatg agtgtgtctt aatgcgtgcg atgtaataat
     9301 tgatatatta ttgactctac aggctgattt caaagcatta ttgatagcat gaaggttaat
     9361 tatagatccg gcttctttga aaatgtaacc atcatagcta attgcaaatg tacttatgac
     9421 gtccataatg tgtttcatat cagatttagc gatactgata tatctagggg aagtatcggt
     9481 ttttcgctcg tcaataaata tagtgttttt cacttggttg atatgctcaa tctttatatt
     9541 tcttgcacca ctgacacgac aacccgtaca aatcattatg aatagcgcta atgatgaacg
     9601 agttctcttc tttctgacgt gatcttttag tatttcatat tcagttaccg agatgaattt
     9661 ttcttgttct gacttcgtag gttttccggc tttataatta actttataag cgggattttt
     9721 aaaaataagc ccatcatata atgcgtcatc taaagctgac cgaatagcac cgtttgtttt
     9781 tcttatagtt tcttttgcgt gttcttttga ataatcgttt atgaatttct gataaacttg
     9841 tctatttatc tttgataact ccattttacc tattttatgt ttttgtatat gttgtaatgc
     9901 atttctataa tgacggtagg tattttcttt aacaacaggt tgtttatacg ttttaatcca
     9961 attttcgaag tattcttcaa gagttatata gttatctata ttaaaaccac ttcttaactc
    10021 atttaacttg tctagtccag cagaattagc ttcacgcttt gttctaaaac ctttcttacg
    10081 gtatcttttt ccttcatact taaattcata ttgccatttt ttaccatcgt aacaacgtgt
    10141 tttcatgcgt tccctcctca aaattggcaa aaaataataa gggtaggcgg gctacccatg
    10201 aaaattgtat aaaaaaagac gcctgtataa aatacagacg ccacttatag ttataagatt
    10261 acatggttaa ttaccaaaaa tggtaacgaa tatatacgtg ttttaaagga taaaccttta
    10321 atatattaaa attatatcat cttatatcag gaatctgcaa tatattatta ttaattctat
    10381 ttatcagtaa cataatatcc gaagaatcta ttactggatt tttaattttt tggggtaaaa
    10441 cttttcttat gcgaaactta ctaatcggct ggaaagaatt tatgcaagcg taactattac
    10501 cttttaattt ttttacctta tcaattgctg atactatgtt attaatgttt ctgtcaattt
    10561 tatttaattt attttcaatt tctaaactat cagatataaa ttcactaaaa taatctttag
    10621 tgatgaattc tgtgttgttt ttttggtatt ttttatcgaa aacttctttt aatatagctg
    10681 aattattttg cgcgctaatt aaatttaaaa acaatcttaa ataatactcc catttcaaat
    10741 caaaattcat ctttaaatac tttttgtttt ctttagaaga taagggaata acatttacta
    10801 tatcttccgt attagaatca tttttattca tcactattgc aaagtgtgaa ttagagaatt
    10861 ctttattaac gtttataccg aaatctacaa aaactatttc tccttgttta aactttggat
    10921 aaaaaccttt atggtttttt tcaccttcaa atctcttgag taaatagtga atatctgaat
    10981 ctaatttttt aaattttgga tttccagaag tttttaattt attaatgcgt ttttctatat
    11041 tatgcgtcat catttctcct ttattctcgc tcacactctc accaccattc aacgtctaca
    11101 cttgtagacg ttttttgatt agtaaaatca taatgaatct tctttggtta acttatcgcc
    11161 atctattttt tgtgaaataa attccaagta tttacgcgca ttatgtgacg ataaatcttt
    11221 aggtaactca taagtgaatg gttgattacc actagttaaa acttcgtata ttacagtttc
    11281 tctttttatt ttgcaattag ttattttcat tataaacttc ctttcaaaca ctgctgaaat
    11341 agacgtcttt tttaaataag cataattaat acttcaattc tttaatccac atatatttaa
    11401 aagtgaggta gtaggtaata aatataagac ttaaagttaa gattgctttt ttcatgtttc
    11461 ataattaaaa cctctgtaaa tttaaggtta gtattatgaa ataatggatt ggtttattct
    11521 ttagtactaa cttcgtagta aattatatag ttcgctaaat tgtatttatc tactatattt
    11581 ttggaataaa caatttcctt ttctttcttc agtaaattat aaaaatctac atcattttcg
    11641 ttagctgatt ctattttggt gatatcagat tgtctaacga ttcttttaga tctgtcaagg
    11701 tatataaatt tccctgattt agaattagtc tttttattca caccgacgta aattgaaagt
    11761 aatatacttt ttccaaaaac agcatcactg tgattatggt tttcatcttc tataactaag
    11821 aatacatgct tttttgtaaa aatttttttg atcatcgtta ttattccttt attaaattta
    11881 ttaagtcttc ttcatttaaa aattgaattt ttgcaccatt tccaacatat tctcgagctt
    11941 ttcgttgttt tgaaactagt ccgttcacat ctttatattt atcatcttga acaccttcga
    12001 ctaaaatatc tgtttttgca gttacgtcac ttctgatata agctcctttc tttctagtta
    12061 atatcattaa atcttgtttt tcagtgtcaa aattacctgt aaaaacaaca tttttatctt
    12121 ttaaaatagg gattacactt tc
//
LOCUS       DANJFZ010000078        11994 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.078, whole genome shotgun sequence.
ACCESSION   DANJFZ010000078 DANJFZ010000000
VERSION     DANJFZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.078"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(16..1089)
                     /locus_tag="PWN45_001870"
     CDS             complement(16..1089)
                     /locus_tag="PWN45_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL0623636.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(1114..2478)
                     /gene="radA"
                     /locus_tag="PWN45_001871"
     CDS             complement(1114..2478)
                     /gene="radA"
                     /locus_tag="PWN45_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL0623637.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(2963..5419)
                     /locus_tag="PWN45_001872"
     CDS             complement(2963..5419)
                     /locus_tag="PWN45_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0623638.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(5433..6440)
                     /locus_tag="PWN45_001873"
     CDS             complement(5433..6440)
                     /locus_tag="PWN45_001873"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDL0623639.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(6430..6996)
                     /locus_tag="PWN45_001874"
     CDS             complement(6430..6996)
                     /locus_tag="PWN45_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDL0623640.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(7015..7476)
                     /locus_tag="PWN45_001875"
     CDS             complement(7015..7476)
                     /locus_tag="PWN45_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL0623641.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            7634..8848
                     /locus_tag="PWN45_001876"
     CDS             7634..8848
                     /locus_tag="PWN45_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0623642.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(9056..9616)
                     /gene="pdxT"
                     /locus_tag="PWN45_001877"
     CDS             complement(9056..9616)
                     /gene="pdxT"
                     /locus_tag="PWN45_001877"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL0623643.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(9620..10507)
                     /gene="pdxS"
                     /locus_tag="PWN45_001878"
     CDS             complement(9620..10507)
                     /gene="pdxS"
                     /locus_tag="PWN45_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL0623644.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            10611..11993
                     /locus_tag="PWN45_001879"
     CDS             10611..11993
                     /locus_tag="PWN45_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0623645.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         3704 a         2271 c         1676 g         4343 t
ORIGIN      
        1 cgtcaactac agtttttata atgatactgt atcttcgatt tttttagcaa aaacaattct
       61 tcctgaagat gtttgcaata agctgactac ttctaaattg acatgactgc caataagatt
      121 tttagcatta tcaacaacta ccatcgtacc atcatctaga tatcctactg cctgaccagg
      181 ctccttaccc atttttgtca gtaaaatatg cagttgatca ccttgatgta cattaggttt
      241 gattgcttct gataaatcat taacatttaa tgctttgata ccatgtacat gacaaacttt
      301 atttaggttg aaatctgtcg ttataatact tgcatgatat tgttttgcta attttaataa
      361 catcgtatca atatcactat gtgttttagt tggatgtata acctttgtag gatagtctaa
      421 atcatacaat tcatttaaaa tatctaagcc tcttttaccc ttttcacgtt taacactgtc
      481 atttgaatct gcaacaattt gtaattcatt aataacacct tgtggaatta aaatattgcc
      541 atcgataaaa ccgcaacgaa tgacttctaa aatacgacca tcaataattg cgcttgtgtc
      601 gataattttt ggcgtagcac ttttagtatg ttgtgacatg gaacgcgcta tattctcagg
      661 taaaaacatt aacatttcat ctcgtttttt aaggccaaat tggaaaccga aataacatag
      721 taatatcgta attatgacag gaatgaaatg attaaaaata gagttgccaa ttgattctaa
      781 tataaacgac accataacag aaataagtaa tccgattatt aaacctattg ttgcgaatag
      841 tatttcaaca gcacttctac gcataataaa atgttctaaa ccttttatag cgttagtaac
      901 tcgtctaata aatacaccaa aaattaagaa cataaaaata ctaccgataa tgccatctac
      961 atagtgattt tttaaaaagc tggagttttg taatccaaga tcatttgcaa tttcaggaat
     1021 aataattatt cctaatgcgc tcccaataat taagtaaata ataataacca ttagtttaac
     1081 gatattcaca caatgtcctc ctttcttgat gttttatgaa tgaagagcaa atgacaatac
     1141 ttcatgtaca gtagttacac ctattacttg tataccttca ggatatgtcc atccgcctat
     1201 attattttta ggaataatta cacgtttgaa acctagtttt gcagcctctt gcacgcgttg
     1261 ttctatccga gatacacgac gtacctcacc cgttaaacca acttctccaa tatagcaatc
     1321 taatccgtcg acagctttat ctttaaagct agatgcagtt gctacaatta cacttaaatc
     1381 aactgctggc tccgttaact ttacaccgcc agctactttg atataagcat cttgttgttg
     1441 taatagataa ttttctttct tttccaaaac agccatcaac aaacttaatc gattatgatc
     1501 aatccctgtt gccattcgtc tcggattgtt aaaagtcgtt ggagttacca gcgcttgaac
     1561 ttctattaaa agtggtctgg ttccctccat ggttgcaaca attgttgaac ctggaacatt
     1621 tgttgaacgt tcttctaaaa acatttcaga tggattattt acacctttta atccactttg
     1681 cttcatttcg aagattccca tttcattcgt tgaaccaaaa cggtttttaa cagctcgcaa
     1741 aattcgatat gcgtggtgtt catcgccttc aaaataaagc acagtatcaa ccatgtgttc
     1801 tagcaatctt gggccagcaa tttgaccttc tttcgttaca tgacccacta taaaagttgc
     1861 aatgttcatt tgtttagcaa tattcattaa actttgtgta ctttcacgaa cttgtgaaac
     1921 agaacctggc gcagagctga tttcaggatg atatattgtt tgaatcgaat ccactactaa
     1981 taaatcaggt tgttcttctt ttactgtttg ataaataact tcaagatctg tttcagctaa
     2041 tacttgcaat tcacttgaat cttcatctaa tcgctctgca cgtaatttag tctgactaag
     2101 cgattcttct ccagtaatat atagtacttt tttcttttga gataacgatg cacaaatttg
     2161 taaaagtaac gttgacttac caatacctgg atccccacca ataagtacta acgatccgct
     2221 cacaatacct ccacctaata cacggttgaa ttctgctgaa tctgttaaca ctctcggcgt
     2281 tgtttcatgt ttaatactat ttaatttttg tactttacct gctaattcct tggttttaac
     2341 tccatgttta ggattggctg ctttttcaac aatttcctcc atttgattcc aagcgccaca
     2401 attaggacat ttccccatcc atttaggaga ttgataacca caagccatac attcaaaaat
     2461 cacttttttc ttggccaaaa ttgcacctcc actttccttt gaacaaatct attttaaacg
     2521 cacattccca ttatgacaaa ttaaatgtga gttacatttg tttgtatttt aacatgacta
     2581 ctaacgcaaa ctgttttacc agatttattt tttatgaata ttataaaata aacttacatc
     2641 atgttgatag tcggtatctt tcaaagataa aatcttaata atttcttagt aaactctttt
     2701 ctctagattt atcacaatat tatatagacc tattttattt tgacgtaagt tgctagtatc
     2761 ttcaaacaaa aaacctttat aaaaattcat acctttatgc tatcgctgta ggctcattaa
     2821 cttgttacat ataattctta actatccttt gatgattgtt ttattagatt gtttcgttga
     2881 tggatacttt cacgaatttc tatagttcaa tgctactaaa aaaacagccc aaaactttaa
     2941 tttgttttgg actgttttat aattatgctt gcgatggtgt tttagtttct gaagtttgtt
     3001 cagcaatgtc atatttaaac tctttaccat catgatctac tgtaactttc ttaccttcaa
     3061 tttgattacc atctaatatt aattcactta aattatcttc gatagttttt tgtatcgctc
     3121 taattaatgg tcttgcacca tattctggat catatccttc ttctgcgatt ttgtctttcg
     3181 ctttatcagt tacaataata tttatgtttt gttcagataa tcgatttgtt aatttattaa
     3241 ccatcattgt tacaatttct tttaattctt cttttgttag tttatggaat acaatgatat
     3301 catctacacg gtttaaaaat tctggacgga atgaattttt taattctttt aacatcgttt
     3361 ttcgaattgt ttcataatct tgtccatcac ttgaaccacc gaatccagca aatcgttgat
     3421 cttgtaattc ttgtgcccca acgtttgatg tcattatgat aattgtattt ctgaaatcaa
     3481 ctgtacgtcc ttttgtatct gtcaaatgtc catcatctaa aacttgtaat agaatattaa
     3541 atacatctgg atgagctttt tcaatttcat caaataaaat tacagaatat ggtttacgtc
     3601 taactttttc agttaattgt ccaccatcat catgaccaac atatcctgga ggagcaccaa
     3661 ctaatcggct cactgcgtgt ttttccataa attcactcat gtctacacgg atcatcgcat
     3721 catcatcgcc aaacattgat tcagctaaag ctctagctaa ttcagtttta ccaacaccag
     3781 ttggtccaag gaagataaag ctaccaattg gtcgtttagg atcttttaac cctgcacggg
     3841 cacgtctaac cgctttactg attgaattaa cagcatcttt ttgcccaata actctctcat
     3901 gtaatgtatc ttctagacta agaagttttt cagattctgt ttcattgatt ttagttaatg
     3961 ggatacctgt ccatcctgca ataacttcag caatatcttc ttctgacaat gaagttgaca
     4021 tgccattttg tgcattcttc cattcatttt tagcttcttc atattgcttt tcaagttttg
     4081 tttgtttatc acgcaggtta gcagcatttt caaactcttg agcatgtact gcggcatctt
     4141 tttcattttt aactttttca atttcttgtt caatttcttt taaattatta ggtgtcgtat
     4201 gactcttaag tcttacttta gaacttgctt catcaattaa atcaattgct ttatctggta
     4261 agaaacgatc tgaaacgtat ctgttactta atttaacagc tgcttcaata gcttcgtctg
     4321 aaatattaat acgatggtgt gcttcgtaac gatctcttaa tccttttaaa atagcaactg
     4381 tatctactac tgaaggttca tcaacttgta caggttggaa acgacgttct aaagccgcgt
     4441 ctttttcaat atttttgcga tattcatcta atgtagtagc accaatacat tgtaattcac
     4501 cacgtgctaa tgccggcttc aaaatattcg aagcatcgat agcaccttca gcaccaccag
     4561 caccaactaa agtatgcaac tcatcaataa ataggatgac attacctgct tgttggattt
     4621 cttccataac ctttttcaga cgctcttcaa attcaccacg atatttagta cctgcaacta
     4681 ctgttcccat atctaaagac ataacacgct tatcttttaa tgtctctggt acctcattat
     4741 tcactatggc ttgcgctaaa ccttcagcaa tagcagtttt accaacacct ggctctccaa
     4801 taagcacagg attgtttttc gtacgtctac ttaatacttc aattacacgt gtaatttctt
     4861 tatcacgtcc tataacagga tctaatgtac cgtctttggc aatgactgtt aagtcacgag
     4921 ctaaactatc taaagttgga gtattatttg acttactagc ttgtgcattt ttattactca
     4981 tttcagggtt tcctaaagct ttcacaactt gtgcacgtgc tttagtaata tttaaatcta
     5041 gatttgcaaa aactcttgct gcaacacctt cattttcacg aatcaagcct aataaaatat
     5101 gttccgttcc aacaaaattg tgatgtaatt ttctagcttc atccatcgat aattcaatga
     5161 cttttttagc tctaggtgta taatgcaatg taccaacatg atcttgacca tgtccgatta
     5221 atttttcaac ttcttcaatt actttatctt cagtgatatt aaaactttct aatacttttg
     5281 cagcaattcc ttcaggttct ttcattaacc ccaataatag gtgttctgtt cctatatttg
     5341 aatgatttaa acgaattgct tcttcttggg catgtgctaa tacgcgctgt gcacgctcag
     5401 ttaatctacc aaataacata aataatgacc tcctacttta tatgttctct tagtatatct
     5461 gctcgttttt cttttacaga tttgtcatct tcttcatcta ataaaaatgg tgactgtata
     5521 gctaccatca attcattaaa tttaaagttt tgtaattcaa tgtaatttaa atctatacca
     5581 agtttaactt cgcttaatct ataagaagcc tcttccatag ttatcattct acagttttgt
     5641 aaaataccta gcgagcgaaa aacacggtct tgtgtttcta attgattata agtgtctaac
     5701 ttttgtcgta tttgtttttc ttcatgaatg atttgattaa caacttctgt taatgtttct
     5761 atgatttcta actcagattt accaagtgta agttggttgg atacttgata agtatgtcca
     5821 taaacttgcg aaccttcacc gtaaatacct ctgattgtat atccaaaacg attaatggtt
     5881 tgagcaatcc gtgtcattct tttcataata gatagacctg gcaaatgtag catcacgctt
     5941 gctctcatac cagtacctat attggtagga catgtagtta aataaccaag ttgttcatca
     6001 taacttatat caaggcttcg atctaattca tcatcaattg atgaagcttg attatataaa
     6061 gcctgtaatg tcgtgtcagt tcccatagct tgaatacgaa tatggtcctc ttcatttatc
     6121 atgacactta aagattcatc gtcattcact aatactgcgg ctgctggttg ttttattagt
     6181 tcaggactaa tcaaatgctt tgcaaccatt ttcattttac tttgttgatc catttgatca
     6241 agacgcatta attcaaagtt tggcaaggca tcttgtacct catttataac tctaaatcca
     6301 tcattttcag tagcatacat tagtggatgc acatgatttt ctaaattacg cgctaaccga
     6361 attctagaag acataacaat tggtgtttct tcattacttt tcatccattg gctgatatta
     6421 tcatgaatat tatgcgtcat catgttgcac ctcactctca gcttttagtg ctttaatttc
     6481 atctctaaca atggctgctt cctcaaaatc ttggatttca ataagttttt tcaaatattc
     6541 attcttttct tcgatttttc gctttaaagc tatcttttta tgtgaagaat gtggtgtctt
     6601 tccaacgtgc tcaaattgtc caccttgaac tctgcggacg atatcaatga tgtcatcttt
     6661 aaatgttgca taacaattag cacacccaaa tttaccaaca tgtgcaatat ctttcaaagt
     6721 catatgacat gatgggcaac gtttttcttc tttaaacgct acttcttgaa aattaattcc
     6781 atgtttcgta gctaaatgtt gtaaaatttg cttaacaaca aatgcttctt cgaaattatc
     6841 ttgatgttct tgatattcag gttgttcatt agcttgattc cacggatggt gtccctcagc
     6901 acaagtttga cacaccattt tttcttctgt tttattttta cttgtaactt taacttttaa
     6961 ttccgcttca ttaagttgac aattttcaca aagcacttca acacctcatt tcatttagta
     7021 ataatttata actggtaaca aacgttttaa aatatttgct ctaataatat ctctagaaac
     7081 catatccatt gatagcgttt ctctatcaat aactgcttga atcattttag cttcacgttc
     7141 atttattaac attttatcta aaagcccatc aataatataa taagcttgtt gttgagaaat
     7201 agaaggtcca atcagctgaa gcaaatgatt aatataacct gttgcatctt tattttcaat
     7261 tttagtgatt cggatgtaac caccaccacc acgtttactt tcgatttcat aaccatgttc
     7321 attagtgaat cgtgttttga ttacataatt taattgtgat ggtacgcaat caaaacgctg
     7381 tgcgatattc gctctctgaa tttcaacgac atcttcattc gactcttcaa ataaacgttt
     7441 gatgtattgt tctatgatgt cagacatatt gtgcatgtat atcacccctt tttgaccttc
     7501 tttgactata ttatatgacc tattttgacc tttttcaatt aatttgtttt tatattttca
     7561 aaaatttatg atgttagccc ttccatttta ttgtatgata gattgtagaa aaaactaaag
     7621 gaatgagaaa tatatgcata ttctaattgg gattatcgga attatcttct ttttagcact
     7681 tgcgtttcta tttagctcag acaggaaaaa tattcgctgg aaatatgtag gaattttact
     7741 tgcaattcag cttatctttg catttatctt gcttaaaact acaatgggaa tcaaggttat
     7801 tggtggtatt tcaaacggtt ttaactactt attattaaaa gcagcagatg gtgttaactt
     7861 tgtattcggt ggtattcaat atatcgatcc taagcaacca ccattcttct tcagtgtatt
     7921 attacctatc gtatttattt cagcgattat cggtatcctt caatacacaa agattttacc
     7981 tttaatcatt aatgttttag gattcttaat ttctaaaatt aatggtatgg gacgtttaga
     8041 atcatacaat gcagttgctg cagcaatttt gggacaatct gaagtattta tttcaattaa
     8101 aaaacaatta ccatacatac ctaagcaacg tttatacaca ttaacagctt cagcaatgtc
     8161 gactgtttct gcttcaatta tcggtgcata ctttacctta attgaaccta aatatgttgt
     8221 aacggctgtt gtcttaaact tatttggtgg tttcatcatc gcttcaatta ttaaccctta
     8281 taaagttaat gaagaagatg acaaattatt agttgaagaa actgaagaaa gacaacaatc
     8341 attcttcgaa atgctaggag aatatatttt agatggattt aaagtagctg tcattgtagg
     8401 cgcaatgtta atcggataca ttgccattat cgctctatta aatggaattg taagcaatat
     8461 ctttagtaca gtttcaggtg gcgctatttc ttgggatttc caaacattaa ttggttttgt
     8521 ctttgcacca tttgcattct tagtcggtgt gccatggcaa gatgcagttc aagccggttc
     8581 agttatggcg acaaaactat tatctaacga atttgtagca atgcaagcac ttggtaaact
     8641 tagtgattta tctgaacacg caaaaggtgt tacatctgta ttcttagtat catttgctaa
     8701 ctttagctct atcggtatta tttcaggtgc gattaaatca cttaatgaca aaaaaggaga
     8761 tacagttgcc cgctttggtt tgaaattatt attcggtgca acactagtat ctttcatttc
     8821 agctgcaatc gctggattct ttatttaaca aatacatcaa catcgaatta accccttcaa
     8881 aattttgact attctcagtc tctttgaagg gtttttttct tatgtatagc tttatttttt
     8941 atcattttta attttgacat aaaaaataac tcctccaaag ctaagtttga aggagatatt
     9001 ttcttctcaa caaatctata aatatcaata attcgttgat ttagcataca ttaagctatg
     9061 ctttttttac aatatgatta ataaagtaat cagttactct atagtcatct gttaattcag
     9121 gatggaatga tacgcctaaa tatttacctt gctgaacagc tacaattttc tcattaacct
     9181 tacataggat atctacgcct tgacctactt tttcaatatg tggggctctt ataaagacac
     9241 cttcaatatc tgtagcgata cctttaatat ctaattctgt ttcaaagctg tcaacttgtc
     9301 taccgaatga gtttcgttgt acagtaatat tcaacttgtt aaggtatcct tcttcaccaa
     9361 ctatatcttg cgctagaact attaatcctg cgcatgtacc aaacataggt aaagttgaat
     9421 tttgtaaagc ctctttaaat ccatataaat tcattaatcg acgtaacgtt gtagactcgc
     9481 caccaggtaa tattaagccc tcgatttctt ctaattgttc aactttttta actgcaatac
     9541 cttcatgacc acttaattca atatgtctaa tatgttcacg tactgcacct tgtaatgcta
     9601 atacacctat tttcatatct taccaaccac gctcttgcat acgttcttct aatgataatt
     9661 gattgatatc taaacctttc atagcagtgc caagttcact tgctaatctt ccaattagtt
     9721 catagtcttg gtaatgtgtt gttgcttgaa caattgcttt agcaaatttt tctggatctt
     9781 ctgatttaaa aatacctgat ccaacgaata caccgtcagc acctaattcc atcattaaag
     9841 cagcatcttg aggagtcgca acgccaccag ctgcaaagtt aactaccggt aaacgaccat
     9901 tgtctttaat ttgttttaaa atttcataag gcgcaccgat atctttcgca aaagtcataa
     9961 tctcatcatc attcattaca gtcaatcgac taacttctga attaacttgt ctcatatgtc
    10021 ttacagcttc aacaatatta cctgtacctg gttcaccttt agtacgtaac atagcagcac
    10081 cttcaccaat tctacgcgca gcttcaccta aattacgaca tccacataca aatggtactg
    10141 taaattgatc ttttcttaag tgatattcct catctgctgg tgttaacact tctgattcat
    10201 caatatagtc aacacccatc gcctctaata ctcttgcttc agtgatatga ccaatacgtg
    10261 ctttagccat gactggaata gaaacagcat tcattacttc ttctacaatt ttagggtttg
    10321 ccatacgtgc aacaccacca gcagctctaa tatcagaagg tactcgttct aatgccataa
    10381 ctgctaccgc gccagcttct tctgcaattc ttgcttgctc agcattaacg acatccataa
    10441 taacgccgcc tttttgcatt tcagccatac ctcttttgac tctgtctgat ccaataattt
    10501 tactcattat acataaaacc ccttttcaag ttgattaaat acattgtaat gtataaactg
    10561 atatttaaaa aggtacaaaa taagataaat ttaggaggtc agttataaaa ttgaaaaaca
    10621 ctttatacca tcaactttat gaaaaattaa aaaaacaaat aattgaaggt caatttaaag
    10681 aaggcgataa attttattct aaaagacaat taagcaaaca tttatctatt agccaaacaa
    10741 ccgtagagca tgcatatcaa cttctattag acgaaggtta catttattca agacctcgct
    10801 ctggatattt tgtgtccgaa attgaatctt taacaatttt aaacaatcaa cctatcccct
    10861 ctctttttga tgatgattca tataaaccaa aagcgagtga tgaagcttat gattatgctt
    10921 ttaatttgga tgaaatagat acgaaacatt tccctataga acttttcaga aagtattcca
    10981 aagatcttta tgatacaaat catttaaatc aattacgtag aggacatttt caaggagaat
    11041 tacatttgcg gtttcaactg gcattttatt tatttactaa tcgtggcgta atttgtgatc
    11101 ctaatcaaat tataattggc tcatctaccg aacaattagt aaatcaatta gtcgatttac
    11161 tttatacttc aacatttatt atcgaaaaac ctagttatcc accaatcaaa aatattttag
    11221 ataaaaaaca agttgaatat gaacaaattg aagttgaaga taatggaata aacgtagatg
    11281 aagttattaa atcacaaaaa aatatagtat atataacgcc ttcccatcaa tttccaactg
    11341 gatatgtgat ggacttaaaa aaacgaactc aattaataca atgggctcaa gaaaaagaag
    11401 aacgatttat tatagaagat gattatgatt ccgagtttag atatttcggc aaacccattc
    11461 cagcaattca aggtctatat tcaagaggag aaaaagtgat ttatatcagt acattttcaa
    11521 agtctatttt tcctagctgt cgagttgcat atatggtgtt accctattct ataatgaaaa
    11581 aatatcattc tcaaaatcac attgaaggca acactgtacc tgttcatatg caaaacctta
    11641 ttgcaacgtt tatatcttcc ggtggttttg aaaggcattt aaataaaatg agacgaattt
    11701 atcgtagaaa actaacctat attttaaaaa gattgaaacc ttacaaagag cagcttgata
    11761 ttcaaggtgc tgaaacgggt atgcatttta caattacagt aaaaaatgga ttaacattac
    11821 aagagtgtct tgatagagca aacaaagtaa agttaaaatt acaagtttat aattttgatg
    11881 atgatcaaga ctataagaaa acccctaaat ttatattagg ctttggcggt atagacgatg
    11941 atgctttaaa accgcatgta gacgctctaa taaaatcatt aattataaag taac
//
LOCUS       DANJFZ010000079        11861 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.079, whole genome shotgun sequence.
ACCESSION   DANJFZ010000079 DANJFZ010000000
VERSION     DANJFZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.079"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(74..766)
                     /locus_tag="PWN45_001880"
     CDS             complement(74..766)
                     /locus_tag="PWN45_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDL0623646.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1151..1948
                     /locus_tag="PWN45_001881"
     CDS             1151..1948
                     /locus_tag="PWN45_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDL0623647.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2081..3028
                     /locus_tag="PWN45_001882"
     CDS             2081..3028
                     /locus_tag="PWN45_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0623648.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            3630..6584
                     /gene="fdhF"
                     /locus_tag="PWN45_001883"
     CDS             3630..6584
                     /gene="fdhF"
                     /locus_tag="PWN45_001883"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDL0623649.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6584..7057
                     /locus_tag="PWN45_001884"
     CDS             6584..7057
                     /locus_tag="PWN45_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDL0623650.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            7285..7518
                     /locus_tag="PWN45_001885"
     CDS             7285..7518
                     /locus_tag="PWN45_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623651.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            7540..8316
                     /locus_tag="PWN45_001886"
     CDS             7540..8316
                     /locus_tag="PWN45_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDL0623652.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            8627..9751
                     /locus_tag="PWN45_001887"
     CDS             8627..9751
                     /locus_tag="PWN45_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL0623653.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(9843..10796)
                     /locus_tag="PWN45_001888"
     CDS             complement(9843..10796)
                     /locus_tag="PWN45_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDL0623654.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(11158..11658)
                     /locus_tag="PWN45_001889"
     CDS             complement(11158..11658)
                     /locus_tag="PWN45_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0623655.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         4207 a         1645 c         2242 g         3767 t
ORIGIN      
        1 aatacgaagt attgtataaa tagagaacag cagcaagata ttttctaatt gaaaatcatc
       61 ttactgctgt tttttaggaa tcgatggggt ttaaaaattg atgatagtta tcaatggata
      121 tgtctctggt ttgaatcgtc ttaccgaatg aatacgaaat atctataata ttttcaattt
      181 cgagttcggc agttttgtct tgagcaatgt cataaatgac tgcatgccaa tgtccatgtt
      241 tacacaaaag gccaataaat ataacgtttt cagctttaat attatcatca accctatgat
      301 ctactaacat cacatttcgt tctgtacaat aaattaaaat gtcggaaaac agcattggga
      361 gtgtcatatg ttgtgtgtca tcaaatttaa tataaaagtc catgcgttta agcaattttc
      421 ttaagcgtgt ttgtggaaga cttaaacatt gttttataat tgcgttgatt tcgcttttat
      481 atggtaatgt cgagtacgac tgactttcat taagcactaa aaataaggct gataattgtg
      541 tttcagataa gtttaatgca attttagatt gtgcttgttg tatttggtaa ccaccatttt
      601 tcccataatg cgcataaatt ttaacacctt gattttctaa atcatcaata tctcttaaaa
      661 ttgtgcgttt ggatacgttg caatatttag ctaattctaa agcggtcatt tttttatttt
      721 gttgaattgc agtaattatt aaattttgcc tttcagcttt attcatgaca gccacctcct
      781 aaaagatgat atattacaca aagtataaca taagagtgac aatgaggaca ttttcaatat
      841 cttttaaaaa aaatcgtaaa aattttgtgg aaaacgctta cattttaaaa aagattgtgc
      901 tatgatatta cttgtaacag ggggagcgat taaacaaagg ggtagagcta tataacagat
      961 agcttttaat cgttcaagtt acatcacaac atcttcaata tttattactt actttccttt
     1021 ctatttgtcg gctagcacgt gactagccga tttttttatg cataaaatga agaagtcttc
     1081 agttgtattg ttgattcttt taatataggg taataataaa atgataatga tatgagaagt
     1141 ggtggacgaa atgacagata aaacattaca gcaaatagat aagttaatat gttcgtggtt
     1201 gaaacaaata gacaatgtta ttccgcaatt aattatggaa atgactacag aaactaagcg
     1261 tcatcgattt gatttagtta caaatgtaga taaacagatt caacaacaat tccaacaatt
     1321 tttagcaaca cattttccgg aacaccaact attagcggaa gaaaaaagta atgaaatgat
     1381 tacgaatgaa attaatcatt tatggattat ggatcccatt gatggaactg ccaatttagt
     1441 aaaacagcaa gaagattatt gtattatatt ggcgtatttt tatgaaggta aaccaatgtt
     1501 atcgtacgta tatgattatc cgcataaaaa gctttataaa gcaatacgag gagaaggtgc
     1561 tttttgtaat ggaattaaga tggaagaacc accatcgtta aaattggaag acgctattat
     1621 atcgtttaat gcacaagtga tgaatctaga tacggtgcaa gatttatttg acgcctcatt
     1681 tagttatcgt ttagttggtg cttgcggatt agattctatg cgagtggcaa aaggtcaatt
     1741 tggggcacat attaatacaa atcctaaacc ttgggacatt gctgcacagt ttttatttgc
     1801 agaactattg aatctaaaaa tgacgacttt agatggtaag gcaattgatc atttaaaggg
     1861 tgcacctttt attataagta ataaagcatg tcatgaaacg gtacttaaaa ttttaaatgc
     1921 aaatggtggt tatcaaaaat acagatagat attctctaga gataaaaaat gataaaaaat
     1981 agttatacgt ctacagaagt atagtaaaaa aattatttta ctatataata aatagagaaa
     2041 acagacaaag tatgtaagga atagtaaagg agtggtggga ttgaaacgtt cgtctaaaag
     2101 taaaatgagc ctaccgaaaa aaatattttt atgggtattc ggtattttag taatcttagc
     2161 gattgtagct gttgtgtatg tagctgctaa aatttttatt actggtaata agatacacaa
     2221 tccgttagat cgcaatcatt cagaattaag agataaaaaa gtgagtttaa acgatggtga
     2281 tccattcaca atcgctttat ttggtgtcga ttcagatgct gatcgtaaga aaaagggtgg
     2341 cggagagcgt agtgatagta ttatgatttt atctatcaac cctaaaacga agaaaactga
     2401 aatagtaagt ataccgcgtg atacaagagc ggaaattgtt ggccgtggta ctactgaaaa
     2461 aattgcacat gcctatgcat atggtggacc aaatatggct gttaaatcac ttgaaaaatt
     2521 aatgaatgtg ccaattgatc attatgcgac aatcgatatg gatggtttgc ataatatgat
     2581 agatagcatc ggcggtgttg atgtagtaag taatgataca ttcactgttg atggtgttcg
     2641 ttttacaaaa ggacagcaga cacatgtaaa tggtgatcaa gcattgaagt ttattagaag
     2701 tcgtaaagaa gaaggtgctg gtggtgactt tggtcgacaa caacgtcagc aaattgtgct
     2761 tgaagcgatg gccaataaga ttgcaagccc atcatcgatt acacatttta atagtctaat
     2821 gaatgaaatt caaaataatg ttaaaacaga tttaacatta ggtgatttga atacgattag
     2881 aagcaattat aaagatgcta atgacacgat taataaacat caattgagtg gtcaaggcgg
     2941 cattcaaagt gacggattgt actacttcat tcctagtgag cagtcaaaag ctgaaagtac
     3001 taagttatta aaagataatc tagagtagta aactaaggga aaataagtta tcaaaattgt
     3061 acacaaatta attagataga atgatctgta aatagaataa cgaatagaac taatagcccg
     3121 taaatagaag gtgataacac tttgtattta tgggctattt tatttaatgc ctgatttgat
     3181 ttttataaat taatccatta caaatttaat ttaaagataa atattacctt ctaaatgata
     3241 gtagatattt atcaaacttg ggttattaat tttcagaaaa agggtattta taaatagtat
     3301 aacgattttt aggtgaggtg attattaaat attgtcatta ataaggttgt gaaagatatg
     3361 gcaaaaagcg tgaaacagat aattggaaaa ctaataatga taattatttt caattaaaca
     3421 aaaagatgat ttaatttcgc gcaaaatatt tcaaataaaa atgattgtaa aaaggcaaaa
     3481 tacaaaattt tacttaacaa ctagtacata aagtaataca attaaattaa ttctatctga
     3541 aagatgtgtg gggcatcgtt attttaggtg gatatgagca atttattaaa agtcatttac
     3601 ggaaaatata tatagacggg gtgagtaata tgcaagaaca tttggtggtt acacttgatg
     3661 gaaaagatta tcttgtagaa ccaggtacga atttacttga atttattaaa tcacaagata
     3721 cttttgtacc ttcaatttgt tataacgagt cgatgggacc aattcaaaca tgtgatacat
     3781 gtactgttga gattgacggt aaaattgaac gttcatgtag tacggtgatt gatcgtccaa
     3841 tgactgtaaa tactgtgaac aatgatgtga aagatgctca aaaagaagcg cttgatcgaa
     3901 ttttagaaaa gcatatgctg tattgtacag tatgtgatta taataatggt gattgtgaaa
     3961 ttcataatac gatggatgca tgggggcttc agcatcaaac gtatgaatat aaagagaagc
     4021 catacgaaaa agattacggc ccattttatc gttatgatcc aaatcaatgt atcttatgtg
     4081 ggcgttgtgt agaagcatgt caagatatcg aagtgaatga aactattaga attgattggg
     4141 atcgtgaaca tccacgtgtt atttgggata atgatgtacc gattaatgag tcttcatgtg
     4201 tatcttgtgg tcaatgtgcg acggtatgtc catgtaatgc catgatggaa gtgaatatgg
     4261 aaggtaatgc gggttatatg actgatacgg aacctggttc attagcagca atgattgatt
     4321 taactaaaaa agcagaacct ggttatggtc cactatttgc aatctcagat tcagaagcag
     4381 aaatgcgtaa agagcgtatt aagaaaacta aaacagtgtg tacttattgt ggtgtgggtt
     4441 gttcatttga agtttggact aaagatagag aaattttaaa agtacaacca tcacatgatt
     4501 ctccagcaaa taaaattgcg acttgtgtta aaggtaagtt ttcatgggga catattaatt
     4561 cagatcaacg attaactaaa ccactagtaa ggaaaaatgg tgagttccat gaagtagaat
     4621 gggatgaagc tttaaacgtc attgcagata attttacagc tattaaagaa aagcatggcc
     4681 cagatgcact atcattcatt tcttcttcta aagcgacgaa tgaagaatcg tatttaatgc
     4741 aaaaattagc aagacaagtt attggcacaa ataacgttga taactgttca agatattgcc
     4801 aagcacctgc aacaaaaggc ttatttagaa cggttggaca cggcggtgac tcaggtagta
     4861 ttgaagattt agaaaaagcg gcaatgtctg tattgatagg tactaataca gccgaagctc
     4921 atccagttat cgcatcacgc atgaaacgtg cacaaaaatt atttggtcaa aaaatacatg
     4981 tatttgatat tagaaaacat gaaatggcag aacgtgcgga tcgtttttat caacctaaac
     5041 caggtacgga tttagcgtgg ttaagtgcag tgactaagta tattattgat catgatttac
     5101 acgataaagc atttattgat gagtgggtag atgattttga tgaatattac aaatcattag
     5161 aaacatttac aatggctttt gctgaagaag caacaggtat tcctgaatca gaattgatta
     5221 aatttgctga agaatgtgct aaagctgaat ctgttgtaat ttgttgggca atgggtatta
     5281 cacaacaaga cattggtagt gactcaagta cagcgatttc aaacttatta ttagtaacag
     5341 gtaattatcg tcgtcctggt actggtgcgt atccattacg tggacataat aatgttcaag
     5401 gatgtagtga tatgggaagt atgcctgata agattactgg ttatcaaagt attgaagcgg
     5461 atgatattcg cgctaaattt gaaaaagaat atggcgttaa attgaatcca aaagctggta
     5521 aagataatca tgaaatggta gaaggtatac atgacggaga agtacactca ttgtacttat
     5581 atggtgaaga tacgggtatt gtggattcaa atattaattt tgtacaagct gcgtttgaaa
     5641 aattagattt catggtagtc caagatgaat ttttaacatt cacagcaaca tacgcagatg
     5701 ttgtattgcc agcaagtcct tcacttgaaa aagacggtac ttttacaaat accgaacgtc
     5761 gtattcaacg tttataccaa gcattagaac ctcttggtga ttcaaaacct gactggaaaa
     5821 ttttccaagc aattgctaat agattaggat ttgattggaa ttacaagcat cctagtgaaa
     5881 ttatggatga agtagcacgc ttaacacctc tatatgctgg ggtaagttat gatcgtttag
     5941 aaggattcaa tagtttacaa tggccagtac aacctgatgg tactgatgag cctattctat
     6001 acttagaagg attcaatttt gataatggta aagcaaaatt attcccatta tcatttgata
     6061 attactttaa gcaagatgaa atttatgata ttcatgtaaa caatggtcga ttgttagagc
     6121 acttccatga aggtaatatg acttatcaaa caccaatgat taaatacaaa gtgccacgtg
     6181 catttgttga aatttctcca gagcttgctg aagatagagg cattcatgaa ggtgcagaag
     6241 ttaagctgat ttctgaaaca ggagaagcgg tattacaagt tcacgtgaca gatcgtgtta
     6301 aaggtaaaga aatctatatt ccattaaata atgatgcgat ggaaaatgga gatctaggtg
     6361 caattaactt attaactaat agtgatgttg atcaatacac ggatacacca tcttataaac
     6421 gtactagctg tcgtttggag gtaattacga aacgtggtaa gtcaccgttg aaccctaata
     6481 atttccgtgt caataaaaaa cgtcaaccgc agtacagtgt tcaagtacag aaaaaatggg
     6541 aacgttcaga ttatgttttc ccgggaaatc aggtggataa ataatggctg agagaatatc
     6601 ttctaaaatt agacgcttag aaaaatcaga agaacaaatt aagttagaaa gtttaaatga
     6661 agttacagag gcaattgcag cgaataaaga tagtatatta aaagcaatca agcttattaa
     6721 aacattagat gatgcaaaac tattagatgc tttaaatggt gcaattcgag gacgtcaagt
     6781 catcataaat aaatttgcag ttgaacttaa taaagatatt tatacagggc tattatctaa
     6841 tatggcttca atggtatttt tattaggtga actcaatgta tcagatttaa gcgacttcct
     6901 aaataaagtt aataaaggat tacacgttgc caatcaagcg agtccaaatg ctaagacaac
     6961 tataagaagt ctattgggcg tattgaaaga tgacgacatg aaccgtagtt taacatacat
     7021 gcttaatatg ctaaaaggta tgtctcgaga agaataacat tacaatgaaa aagtgtttct
     7081 acatgcaagc agtgtaggag cacttttttt aatttttgta aaaattaaaa agggtgtttt
     7141 tgatggaata aatgaataat attgaaaaac aaaaaaatca cgtgctattg tgaatcaatc
     7201 atcgacaggc ctatgaaaca tcatgtatta ttatgattat gtaagttatt taaataaaaa
     7261 gggtgagtgc atattaattc gagaatgtta aaaacgtcag ctgtaatttc taatacatta
     7321 ttagtcatag gtttagtctg tctatttatg atgaaattag tgttagctat tacttttttt
     7381 gctgtttcat tatcaattag tctagttgtc tttaatatga tgttcagaga tagaacaacg
     7441 atgaaagtaa tcgttaacgc ttctttttta atcgtaatat tagcgattgt agtggcatat
     7501 tttttattgt ctaagtaaga aaaatgaggt tttgtctata tgaagaagaa tttcaagtta
     7561 cgcatttcaa cgctactatt gatagttatt ttagttgttt ttgctgtatt actcatcgtg
     7621 aatgaaacta aattgtttaa aaatgatgtg aattactctt ttgatgaggc tgtttcaatg
     7681 caacaaggga aaggtattgt acagacaaaa gaagaggatg gtaaatttgt tgaagcaaat
     7741 aataatgaaa ttgctaaagc aatgactatt tcacataaag acaatgatat gaagtatatg
     7801 gatatcacag aaaaagtgcc aatgtcggaa tctgaagtta accaattgct aaaaggtaag
     7861 gggattttag aaaatcgagg gaaagttttt ctagaagctc aagaaaaata tgaggttaat
     7921 gtcatttatc ttgttagcca tgcattagta gaaacaggta acggcaaatc agaattagca
     7981 aaaggcatta aagatgggaa aaaacgctat tacaactttt ttggtatagg agcattcgat
     8041 agtagtgctg ttcgtagtgg gaaaagttat gctgaaaagg aacaatggac atcaccagat
     8101 aaggcgatta ttggtggtgc aaagttcatt cgtaatgaat attttgaaaa caatcaactg
     8161 aatttatatc aaatgcgatg gaatccagaa aatcctgcgc aacatcaata tgcgagtgac
     8221 attcgctggg cagataaaat tgccaaatta atggataaat cctataagca gtttggtata
     8281 aagaaagatg atattagaca aacatattat aaataagaca tcggtgctta aaggagctgg
     8341 aacaatttat tgtttcgagc tcctttagcg cattctgagt gtgttagtta aatggatttt
     8401 aacctaacaa aaaacgctat atagcatcaa atatgctata tcccacatca ttgttacaaa
     8461 tgtacatgat gtaaatgaat attgctgtct aaatgtgcat gtaatataca atggtgcaga
     8521 taatacactt aagtccttaa aaatgaaacg ttagttccaa gagtcatttt taaacaatag
     8581 tgcatgtgat aaaatagaaa agaatgaaaa atatagaggt gacaatatga agatagcaat
     8641 tataggtgca ggcatcggtg gattaacagc tgctgcatta ttacaagaac aaggtcatac
     8701 tattaaagtc tttgaaaaaa atgagtcagt taaagaaatt ggcgctggga ttggtatcgg
     8761 agataatgtg cttaaaaaac taggtaatca tgacttagct aaaggtatta aaaatgctgg
     8821 gcaaatctta tctacaatga cagtgttaga tgacaaagat cgcctgttaa ctactgttaa
     8881 attaaaaagt aatacattga atgtgacgtt accacgccaa acattaattg acattattaa
     8941 atcttatgta aaagatgacg caatatttac aaatcatgaa gtcacgcata tagataatga
     9001 gacagataaa gttaccatac atttcgcgga acaagaaagt gaagcatttg atttatgtat
     9061 tggtgctgat ggaattcatt ctaaagtgag acaatctgta aatgctgaca gtaaagtatt
     9121 atatcaaggg tatacatgct ttagaggttt aattgatgat attgatttaa agcatccaga
     9181 ttgtgcaaaa gaatactggg gaagaaaagg aagagtaggt attgttccgt tattaaataa
     9241 tcaagcatat tggttcatta caattaactc gaaggaaaac aatcataaat atagttcgtt
     9301 tggtaaacct catttgcaag catactttaa tcactatcca aatgaagtta gagagatctt
     9361 agacaaacaa agtgaaacag gtatcttatt gcataatatt tatgatttga aaccactcaa
     9421 atcttttgtt tatggtcgta ctattttact aggagatgca gcacatgcga caacgcctaa
     9481 tatggggcaa ggtgctggac aagcaatgga agatgctatc gtattagtaa attgttttaa
     9541 cgcatacgac tttgaaaaag cattacagcg ttatgataaa atacgtgtca aacatactgc
     9601 aaaagtaatt aagcgttcta gaaaaatcgg taaaattgcc caatatcgta gtcgtttatt
     9661 tgttgcagtt agaaatcgta ttatgaaaat gatgccaaat gcattagcag ctggacaaac
     9721 taaattctta tataaatcga aagaaaaata atacaacaat atgaaaaccc ccgtatgttg
     9781 aaacgagagc tcaacatatg ggggttcttg tttttataat gttattataa taaattcaat
     9841 tattagttaa cgacaaattg tggtttctca ccttgaacgg cactaattgc agcattagca
     9901 acaattttag acatcatgtc acgtgcttca aatgtagcat taccaatatg cggtgttaat
     9961 actacattat taagtgattt taagtcatcg gtaatatctg gttcaaattc atatacatca
    10021 agtgcagcac cttcaatttc attatctttc aatgcttgca ctagtgcttg ttcgtgcacg
    10081 attggaccac gagaggcatt gattaaatac gccgtagatt tcatcatttt aaattgttct
    10141 gtatcaatta aatgatgcat tttaggatta taagcagcgt tgatagtgat aaaatctgca
    10201 ttctttaata gtgtatctaa atctacatat tttgcaccga tttctcgttc tttttcttct
    10261 ttgcgattag gtccagtgta tagcacatcc atgtcaaatg ctcttgcacg acgagctact
    10321 gcactaccaa tttcacctaa accgataatg ccgattgttt tcccagatac ttctctacct
    10381 ctgaaaaata aaggtgccca tccatcaaat ccagttgtac gtgataattg gtccccttca
    10441 acaatacgac gcgctactgc aagtactaat ccaattgtta aatcagcagt cgcgtttgtt
    10501 gatgctttag gtgtgtttgt aacatctata cttttttctc gggcatactc gatatcaata
    10561 ttattaaaac cagcgccata gttggcaatg atttttaagt ctttaccagc atcgataaca
    10621 tctttatcaa cgtttgtaga taataaacta attaaggcag tcgcgttttt aacaccttta
    10681 attaaagtgt ctttatcgac taatccttta ccttcataca tttcaacttc aaaatgttct
    10741 tgtaaaagtt ttaaacctac ttctggtatt gcaccagcaa cataaacttt ttccataaaa
    10801 gatcactcct tttatcttag tatagtagaa gattagacag tatacaacta tgtcatgatg
    10861 tcttgtgtat caatgatgta agcgcgtact tttgatggag gtgatataac ttaggcactg
    10921 tagaactatg aatattgtaa tgtggaaaaa ctggatcaat taaattagat aacgtagttt
    10981 taaagttaat agtattagaa aaaattaata ttttgaatat gggaggaaat ataaataagt
    11041 aggtggcaac gaaaaatagc aaaaaaagag cttctcctat aaaggaaagc tcaaagtttt
    11101 ttgatgacat atgtactaga attaagtttc aagacaatat gtatcatcgt gtttatatta
    11161 aatatggatg tagttgtagt tacctgcttc acttgcagaa atagttctag aacttactga
    11221 gaaaggtccg ccactataat tcatttctga aattgtaact gaaccatcac tgtttacact
    11281 ttctacatat gcaacgtgac caaatggtcc ttcagaagat tgtaggatag cgcctttaga
    11341 aggtgtatga tttactgtga atccagcacc ttgtgcagca gcagcccaat tattagcatt
    11401 tccccaagta gaaccgattt ctccgccaac tttatcatat acataccaag tacattgtcc
    11461 tgcagtgtat aagttaccag aatgtgaaat tgatgatgta gttgtcgtag ttgtcgtagt
    11521 cgttgtagtt tgagtcgtgt tgtagttata gttgttgtaa tttgtataat tttcagcagc
    11581 atctgcatga tgtgcttgac ctactaatgc tgtgccgatt cctgctgtta acgtagttgc
    11641 tgttactaat tttttcatga ataaagtcct ccaaagttct atatcttttt ttataaataa
    11701 aacgtagcga ctgttttatt ctcacatctc gaattgatga caatagttac tttaacaaaa
    11761 ttaatgcttc ttgtggggaa tgttattgat ttgtaaaaga ataaaaaaac tttgactaat
    11821 tttgtaataa aaattagtca aagttacaat gagattaaca g
//
LOCUS       DANJFZ010000080        11786 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.080, whole genome shotgun sequence.
ACCESSION   DANJFZ010000080 DANJFZ010000000
VERSION     DANJFZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.080"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(410..1162)
                     /locus_tag="PWN45_001890"
     CDS             complement(410..1162)
                     /locus_tag="PWN45_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDL0623656.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            1330..2316
                     /gene="pta"
                     /locus_tag="PWN45_001891"
     CDS             1330..2316
                     /gene="pta"
                     /locus_tag="PWN45_001891"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL0623657.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2319..3155
                     /locus_tag="PWN45_001892"
     CDS             2319..3155
                     /locus_tag="PWN45_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL0623658.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            3742..4662
                     /gene="mvk"
                     /locus_tag="PWN45_001893"
     CDS             3742..4662
                     /gene="mvk"
                     /locus_tag="PWN45_001893"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL0623659.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            4667..5650
                     /gene="mvaD"
                     /locus_tag="PWN45_001894"
     CDS             4667..5650
                     /gene="mvaD"
                     /locus_tag="PWN45_001894"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL0623660.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            5663..6739
                     /locus_tag="PWN45_001895"
     CDS             5663..6739
                     /locus_tag="PWN45_001895"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL0623661.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            6916..7257
                     /locus_tag="PWN45_001896"
     CDS             6916..7257
                     /locus_tag="PWN45_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDL0623662.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(7309..8631)
                     /locus_tag="PWN45_001897"
     CDS             complement(7309..8631)
                     /locus_tag="PWN45_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL0623663.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(8618..9058)
                     /locus_tag="PWN45_001898"
     CDS             complement(8618..9058)
                     /locus_tag="PWN45_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL0623664.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            9116..9217
                     /locus_tag="PWN45_001899"
     CDS             9116..9217
                     /locus_tag="PWN45_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623665.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            9683..11092
                     /locus_tag="PWN45_001900"
     CDS             9683..11092
                     /locus_tag="PWN45_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0623666.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            11096..11722
                     /locus_tag="PWN45_001901"
     CDS             11096..11722
                     /locus_tag="PWN45_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0623667.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
BASE COUNT         4174 a         1646 c         2234 g         3732 t
ORIGIN      
        1 tttagtagtt aaaattctct tgaagtataa aaaggaaaaa ttattgaaaa aaattcgaca
       61 aaaatatgtt tcaaatattt caaataggtt aaaataatat tagttaacca ttacaaaaat
      121 tgtatagagt agcgactgta taatttctat tgaggttaac gtttatatgt agtgatagta
      181 gttaaagttc tcccaaggaa gactactcgg gtacactttg ctatgagcaa agtgtacttt
      241 gttattgata atacattagc acatatatat aagtttaaac atacagattt caactattta
      301 ccaaatcacg tcttatgtat acgggaatat actgaagata aacgaaaaat tccaagctta
      361 aaccgataag cttggaattt ttttattaat ttataaacgt accaatgtat taagaaatcg
      421 caaagaattg atcgaattcg tttgtgttaa taatatgtcc tacaaagaaa ctaccgaatt
      481 caccgtatcg tgctgttgtt tcatcaaagc gcatttcgta tacaattttt ttgaattgta
      541 atacgtcatc tgagaacaat gttacgcccc attcgaaatc atcaaaccct acagaaccag
      601 taataaattg tttgattttg ccagcatatt ttctaccaat cataccatgg tcatacatta
      661 atttttggcg ttcttccata gttaacatgt accagttata agtttcatta cgacgtttgt
      721 tcattggata gaaacaaata taatcagaat gtggtaattc tgggtataat cttgctttga
      781 tatgagggtt ctcataagga tcttcatcag atttaccagc taaataattg ctcaattcaa
      841 tgactgatac atatgaatat gtagggatta ggaagtcagc aatgcgcaat ttgttaaatt
      901 cattttcaat atgatttaaa gacttcattt caggacgtaa gaaccataat aacaaatctg
      961 ctttttgacc agttatatta taaatagctt gatcaccaga ttttgatgat cttacagttg
     1021 ctgtattttc taaaaatgat tgaaattcag tgacaagtgc atcgcgttcg tcctttggaa
     1081 ctatacgtaa tgatgcccaa tcaactgcat aaaataaatg tagactatac caaccatcta
     1141 atgtttcggc tgcttgactc atgtttatcg ctccttttca aaaatcatca gttctttata
     1201 aattttatca taaagcaaag gggtaacgat aaaattatga ttacaaattg gtgacgtggc
     1261 attatgaaat aaaatggcgt ataattatac cgtgaatgat taataagatt tatattacag
     1321 gaggacatta tggctgattt attaaatgta ttaaaagaca aactttctgg taaaaacgtt
     1381 aaaatcgtat tacctgaagg agaggacgaa cgtgttctaa cagctgcaac acaattacaa
     1441 gcaacagatt atgttacacc aatcgtgtta ggtgatgaga ctaaggttca atctttagcg
     1501 caaaaacttg atcttgatat ttctaatatt gaattaatta atcctgcgac aagtgaattg
     1561 aaagctgaat tagttcaatc atttgttgaa cgacgtaaag gtaaagcgac tgaagaacaa
     1621 gcacaagaat tattaaacaa tgtgaactac ttcggtacaa tgcttgttta tgctggtaaa
     1681 gcagatggtt tagttagtgg tgcagcacat tcaacaggcg acactgtgcg tccagcttta
     1741 caaatcatca aaacgaaacc aggtgtatca agaacatcag gtatcttctt tatgattaaa
     1801 ggtgatgaac aatacatctt tggtgattgt gcaatcaatc cagaacttga ttcacaagga
     1861 cttgcagaaa ttgcagtaga aagtgcaaaa tcagcattaa gctttggcat ggatccaaaa
     1921 gttgcaatgt taagcttttc aacaaaaggg tctgctaaat cagacgacgt gacaaaagtt
     1981 caagaagctg tcaaattagc acaacaaaaa gctgaagaag aaaaattaga agcaatcatt
     2041 gatggcgaat tccaatttga tgctgcgatt gtaccaggtg ttgctgagaa aaaagcgcca
     2101 ggtgctaaat tacaaggtga tgcaaatgtc tttgtattcc caagtttaga agctggtaat
     2161 attggttaca aaattgcaca acgtttaggt ggatatgatg cagttggtcc agtattacaa
     2221 ggtttaaatt ctccagtaaa tgacttatca cgtggctgct caattgaaga tgtatacaat
     2281 ctttcaatta ttacagcagc gcaagcctta caataacgat ggatttagcg agtaaatatt
     2341 ttaatggcgt caactggcga tatatcgatc attcttctgg attagaacct atgcaatctt
     2401 tcgcattcga tgatacattt tgcgaaagtg tgggcaaaga tatatcagat aatgttgtgc
     2461 gtacttggat tcatcaacat actgttattc ttggtattca tgattcaaga ttgccgtttt
     2521 taaaagatgg cattgattat ttaacgaatg agattggtta taatgccatt gttagaaatt
     2581 ctggtggctt aggtgtcgtt ctagatcaag gtgtattaaa tatatcgctg atgttcaaag
     2641 gacaaacaga aacaacgatt gatgaagcgt ttactgtgat gtacctctta attagcaaaa
     2701 tgttcgaaaa tgagaatgtt gatattgata cgatggaaat tgaacattct tattgcccag
     2761 gaaaatttga cttaagtatc gatggtaaga aatttgcagg catatcgcaa cgaagagtta
     2821 gaggcggtat tgctgtacaa atttatcttt gtgttgaagg ctctggttca gaacgtgcat
     2881 tgatgatgca aacattttat gaacatgctt taaaaggtga agtgactaaa tttaaatatc
     2941 ctgaaattga accatcttgt atggcctcat tagagacatt gcttaacaaa acgattactg
     3001 ttcaagatgt aatgttttta ctattatatg caatcaaaga tcttggcggt gtattaaata
     3061 tgacgccaat tactcaagaa gaatggcaaa gatacgatac gtattttgat aaaatgattg
     3121 aaagaaacaa gaaaatgata gatcaaatgc aatagttaaa tatttaacag cccctttcaa
     3181 gttatcgttt ggggctgtta tttttgaaag tatccataaa aagcataaat atgttattga
     3241 cctaatattt aaattgaaca tcattaaatt aaaagtaatg atatgatgga aataataaat
     3301 ttaataagaa aatttcatgt tttacgtatt aaatagattg agaatagggt atatataatg
     3361 cgtatgacta tgaaagaaac gaccttctat tgaaatgttc taatacttta gtagtaaatc
     3421 tatatattcc aattattatt tgtagtatta tagttagggt ggaaaattat aaattgtagg
     3481 gctattttat aaaaagataa agaatctgaa atatcactcg ataagattta aactttgaag
     3541 taaacgaaag ttagttttat actaatcaag ttaagtcaaa tataaattca aaataaagat
     3601 tatacataat atgaataatg tgagataaag actgcaatga aatttaacga gaatgcgtat
     3661 gcaacttaga atatgatgtc ttttactaat aaataaaaat aatgaatcaa gtataggata
     3721 aatataaaag gtgatattga catgacaaga aaaggatatg gggaatcgac aggtaagatt
     3781 attttaatag gagaacatgc tgttacattt ggagagcctg ctattgcagt accgtttaac
     3841 gcaggtaaaa tcaaagtttt aatagaagcc ttagagagcg ggaactattc gtctattaaa
     3901 agcgatgttt acgatggtat gttatatgat gcgcctgacc atcttaagtc tttggtgaac
     3961 cgttttgtag aattaaataa tattacagag ccgctagcag taacgatcca aacgaattta
     4021 ccaccatcac gtggattagg atcgagtgca gctgtcgcgg ttgcttttgt tcgtgcaagt
     4081 tatgattttt tagggaaatc attaacgaaa gaagaactca ttgaaaaggc taattgggca
     4141 gagcaaattg cacatggtaa accaagtggt attgatacgc aaacgattgt atcaggcaaa
     4201 ccagtttggt tccaaaaagg tcatgctgaa acgttgaaaa cgttaagttt agacggctat
     4261 atggttgtta tagatactgg tgtgaaaggt tcaacaagac aagcagtaga agatgttcat
     4321 aaactttgtg aggaccctca gtacatgtca catgtaaaac atatcggtaa gttagtttta
     4381 cgtgcgagtg atgtgattga acatcataac tttgaagcct tagcggatat ttttaatgaa
     4441 tgtcatgcgg atttaaaggc gttgacagtt agtcatgata aaatagaaca attaatgaaa
     4501 attggtaaag aaaatggtgc gattgctgga aaacttactg gcgctggtcg tggtggaagt
     4561 atgttattgc ttgccaaaga tttaccaaca gcgaaaaata ttgtaaaagc tgtagaaaaa
     4621 gctggtgcag cacatacttg gattgagaat ttaggaggtt aatgcgttga ttaaaagtgg
     4681 caaagcacgt gcacatacga atattgcact tataaaatat tggggtaaaa aagatgaagc
     4741 actaatcatt ccaatgaata atagcatatc tgttacatta gaaaaatttt acactgaaac
     4801 gaaagtcact tttaacgacc agttaacaca ggatcaattt tggttgaatg gtgaaaaggt
     4861 tagtggcaaa gaattagaga aaatttcaaa atatatggat attgtcagaa atagagctgg
     4921 catcgattgg tatgcagaaa ttgaaagcga caattttgta ccaacagcag cagggttggc
     4981 ttcatcggca agcgcatatg cagctttagc agcagcttgt aatcaagcgc tagacatgca
     5041 gctgtcagat aaggatttat cgagattggc gcgaattggt tcgggttctg cgtcgcgtag
     5101 tatttatggt ggatttgcag aatgggaaaa agggtatagt gatgagacgt catatgccgt
     5161 tccacttgaa tcgaatcatt ttgaagatga ccttgccatg atatttgttg tgattaatca
     5221 acattctaaa aaggtaccta gtcgatatgg tatgtcattg acacgaaaca catcaaggtt
     5281 ttatcaatat tggttagatc atattgatga agatttagct gaagcaaaag cagcgattca
     5341 agacaaagat tttaaacgcc ttggtgaagt aattgaagaa aatggtttgc gtatgcatgc
     5401 cacgaatcta ggatcaacac cgccgttcac atatcttgtg caagaaagtt atgatgtcat
     5461 ggcgcttgtt cacgaatgcc gagaagcggg gtatccgtgt tattttacaa tggatgcggg
     5521 acctaatgtg aaaatacttg tagaaaagaa aaacaagcaa cagattatag ataaattatt
     5581 aacacagttt gataataacc aaattattga tagtgacatt attgccacag gaattgaaat
     5641 aattgagtaa ggaagagata aaatgattca ggtcaaagca cccggaaaac tttatattgc
     5701 tggagaatat gctgtaacag aaccaggata taaatctgta cttattgcgt tagatcgttt
     5761 tgtaactgct actattgaag aaacagacca atataaaggt accattcatt caaaagcatt
     5821 acatcataac ccagttacat ttagtagaga tgaagatagt attgtcattt cagatccaca
     5881 tgcagcaaaa caattaaatt atgtggtcac agctattgaa atatttgaac aatacgcgaa
     5941 aagttgcgat atagcgatga agcattttca tctgactatt gatagtaatt tagatgattc
     6001 aaatggtcat aaatatggat taggttcaag tgcagcagta cttgtgtcag ttataaaagt
     6061 attaaatgaa ttttatgata tgaagttatc taatttatac atttataaac tagcagtgat
     6121 tgcaaatatg aagttacaaa gtttaagttc atgcggagat attgctgtga gtgtatatag
     6181 tggatggcta gcgtatagta cttttgatca tgaatgggtt aagcatcaaa ttgaagatac
     6241 tacggttgaa gaagttttaa tcaaaaactg gcctggattg cacatcgaac cattacaagc
     6301 acctgaaaat atggaagtac ttatcggttg gactggctca ccggcgtcat caccacactt
     6361 tgttagcgaa gtgaaacgtt tgaaatcaga tccttcattt tacggtgact tcttagaaga
     6421 ttcacatcgt tgtgttgaaa aacttattca tgcttttaaa acaaataaca ttaaaggtgt
     6481 gcaaaagatg gtgcgtcaga atcgtacaat tattcaacgt atggataaag aagctacagt
     6541 tgatatagaa actgaaaagc taaaatattt gtgtgatatt gctgaaaagt atcacggcgc
     6601 atctaaaaca tcaggcgctg gtggtggaga ctgtggtatt acaattatca ataaagatgt
     6661 agataaagaa aaaatttatg atgaatggac aaaacatggt attaaaccat taaaatttaa
     6721 tatttatcat gggcaataaa tgatttgata ggggagcaat catctaattt aattagaaga
     6781 taaatgctcc attttttatt gtcagccgtg ttacgtatct tgaaaaaaat attaaaaaga
     6841 gtaaaataga tagagttaac gaaaaaatta atgaaatcga cgatatagat atgataaaga
     6901 aaggtgggta gcaatatgaa aaatacattc cttatttgtg atgaatgtca ggcagtcaat
     6961 ataagaacgt tacaaaagaa gttggaaaaa ttagatcccg atgctgaaat cgtgataggt
     7021 tgtcaatctt attgtggacc tggacgccga aaaacattca cttttgttaa taaccgccca
     7081 ctggctgcgc ttactgaaga agaattaatc gaaaaagttt ctcaacaatt aaagaaacca
     7141 cgtgatcctg aagaagaaga gcgtttaaga aaacgacatg aagaacgtaa acgtcgtaaa
     7201 gtagaacaag atagaaagct taaagaaaaa ttagaaaagc gaaaagcaca acaataaagc
     7261 ctgatggcag catcattcaa tgcgtgccac caggttttta tgttttgtct agaaattaaa
     7321 taaatcatta aatgattcgg ccatcgtagg atgcgtataa atattatctc gtaatacggt
     7381 atatggaatg ttttgatcaa tcgcaagttt aattatatta attaattctt cagattgctt
     7441 accatataat gtagcaccta aaatcatatt attttcatta ttaatgacta ctttaaataa
     7501 acctcttgga tcattgttaa ttttatgacg aggtatagca cttactaaaa gttgatgttc
     7561 agtgtaatca taatgttgag cggcagcttc tttactagtt aatccaacac gtgataatgg
     7621 tggatctata aatactgtat aaggcacgct gcctctattg tcagtcgtac gtgactgatt
     7681 accatataac gctgatttga taattcgata atcatctaaa gatatatacg taaattgaag
     7741 tccgccttta acatcacctg cagcataaat atgcggcaca gttgtttgaa gatgagcatt
     7801 gactttaatt tcgcctctgt cgcctaattc gatatcagta ttttctaaag ctaaatccgt
     7861 attcggtttg cgcccgatag ccaaaagtac tgcatcagcc tcaaagttat caacgttggt
     7921 atggactgtt gtatgatgat tgtcagatga caattcagtc gtttcaacat ttgtatgcaa
     7981 tgcaatgcct ttattttcta agtcagtaat accatatgca acgacatctt gatcttcgcg
     8041 tggcataaat gattcgccac gttctaatac tgttacctta ctacctaaat tcgcaaacat
     8101 tgaagcaaat tctaaggcga tataaccgcc acctacaata acgaggtgct taggttgata
     8161 gctaatgttt aataaacctg tcgaatcgaa gacgtgttta gcttgatcaa ggcctttaat
     8221 gttaggaatg acagaggtag caccggtatt aataatgata tgaggtgcag taatactatc
     8281 gacgatatcg tcatgttgat ctaataaatt cacttcagta ttagatttaa actgcgcttt
     8341 aaaatccagt acatcaatgt tgttatcgtc tgctaataag tggtaatttt tattgtttag
     8401 cgcattgaca acatcgtttt tacggttata acttgcttca aaagatttgc cttctaatcc
     8461 atcatgtaca agtgtcttcg aaggtataca tcctatgttt atacaagtgc ctccatacat
     8521 tttcggagat tgttcgataa ctgcgacgtg ttgacctgtt gatgcagcgt atttcgctaa
     8581 agttttacca gctttcccaa atcctattac aattaaatca tatgttttca tgacataaat
     8641 cctccttttg aatgtcttca atgacatctt tgattgtttt tccattataa aaattaataa
     8701 tgatattctg ttcgtcttgc tgataatgtg acatggtagt tgcaatatta cgagcaattt
     8761 gacagtgact gccttcgtcg ccagtaaata gacgtgtgtg gtgttctttc tctaagacaa
     8821 aatgtttata taatgttgct agagagacat cagcactttg atcatttgct aaataaccgc
     8881 catctttacc tcgtattgtg tcaatcattt ttaaatcgac aagttgagtc gtcacgcgtc
     8941 gtaattgaac aggatttaaa caagttaatt ctgctaatga actactattg aatttttctg
     9001 aatgatgctt agttaaaaaa gctaatacat gcacggcaat gttaaattct aaattcaatg
     9061 tttctcacct cataattgta actgtattag ttacaattaa aaatcatttt gctcaatgtg
     9121 tcaatactta tgtttgaaaa gtgttgaatt aggtgtggtt attgtgatga tttattgttt
     9181 gaaagttgat gcattaacgg tgagattgca caattgaaat tttaattatg tatgaactga
     9241 tagatatgaa atgaatattg aaaatgagag caataaaatt ttgaatgatg atgttgtgtg
     9301 attgtataaa cttattcgta ttttagatac gttttgatat ttcgcatgat gaggaaatac
     9361 ttttaacaac tgatgacata ttttaatagt gaatatattt tgagatatta atatatttaa
     9421 tgttaaggat gttgtcgcaa taaaattaaa atattgaaag ataattttta ttgattaatt
     9481 gcgaatatac aaagacacca ttatatccat atgctaaaat aagaaaaaag gcgacatgtt
     9541 atctcaattt acctgcatct agactccttt acataagaaa taatcatcgc cgagttttaa
     9601 aattttacaa ctttgtagat gtaaaaagca tttgatttta aatagtttga aaatcatata
     9661 tttttgaaaa aggagcatgc cgatgagtat tgacatgtat ttagacagat ctcgaaacca
     9721 agcttcaagt gtggggaatt tgagtcaaac aatgaattca aattatgatg cgttggaaaa
     9781 agcaattact caatttatta atgatgatgc gcttaaaggg aaagcgtata cgtcagctaa
     9841 gcaatttttt agtacggtgt taattccatt atcaacaagt atgaaaacat tgagtgattt
     9901 aacgaagcaa gcttgcgata attttgtgtc acgttatacg agtgaggttg atagcatatc
     9961 tttaaaagaa tcagagcttg aagaagatat cagatcatta agtcaacaaa ttacgcgata
    10021 tgaaaatttg aataacaatt tgaaaaagca tgcttccgat aatcagcaag ccatttcatc
    10081 gaaccaacaa ataatacgaa cattaggtca acaaaaacat gaattagaag agaagctacg
    10141 caaattgcgt gagtttaatc aaaaatcacc agaaatattt aaagaagttg aagaatttca
    10201 aaaaattgtc caacaaggac ttacccaagc gcagaatttt tggaactttt caacaaatca
    10261 atttaatatt ccttcaggta aagaacttga ttgggccaaa gcaagtcatg aaaaatattt
    10321 gaaagttgct atggggaaaa ttgaacataa agcagagaaa gaaactttaa ataaagcaga
    10381 ctttgctgtt ataaaggcat atgccaaaga acatccagaa gacgatatcc cgaaaagtat
    10441 attgaaatat ataaatgaca ataaagacag tattaaaaga gatataggat tagatattac
    10501 gtcaacactt ttagagcaag gcggtataaa tgcaagtaaa ttcggtgtat ttatcaatac
    10561 agcaggtgga gtgaaaggcc cagcaggtcc aaattcattt gtggaagtca aacgtacatc
    10621 aggtaatgtg tttatagaaa atggtagtaa atttgcaaaa ggcggaaaat acctaggtaa
    10681 aggtgttgct ggtgtaggat ttggtatagg tatgtatgat gaccttgcaa atgatgataa
    10741 aacatttgga gaggcgttgt cgcataatgg tatgacgctt gcagctggat ctgcagggac
    10801 agcagttgga gctggattag cgacttttgt tttaggaagt aatccagtag gatgggtgat
    10861 tttagcaggt ttggctatga gtacagtatt tgcattagga acagatttaa tttatcaaaa
    10921 taatattttt ggattaaaag ataaagtaga ctgggtaggg cataaaatcg ataatagtat
    10981 agatgttgtt aaaaaaacta cagaaaaatc catggatagt gttgggaatg ctgttagtga
    11041 agctaaaaat attattagca atcatataaa tccaatgaaa tgggcgtggt gagttttgct
    11101 ttttaatatt aaggttatta ataaaaatcc gagatataaa gtcgttcaat ataatgatga
    11161 gtatttgtta attgatttag taagcacttg gcttgtatac ttttttcctt ttattaattg
    11221 gttcattccc aaaaggtatg caaaacttag tgagaaggaa cttgaaaatt taaatgttga
    11281 taaacaaaat aaaaataata ttttctggcc agttactggt agttcgtttt tatttgtagt
    11341 tattttacgg aaatatgtac atacgtttga agttcaatta gataataaga ttttaatatc
    11401 tttatgtttt ataggattta taggtattgc agcattttac atttacttaa ataaaaagct
    11461 taaactaaag atatatgatg ataatctaga taatgaaaat agggttatat tagtgcctac
    11521 ttttaaagat ggaagtttta tagttttcac atatctatta ttaggaggtt gttctatatt
    11581 atttttaata tggctaatga ctataaaacc tcaaaatcta ctggtattta ttatgtggat
    11641 tattattaca atttttttct ttctaataag catgggctca attagtaata aaaaggttta
    11701 tgccaaactt aaaaagcaat agaaattact aaacttagat tgtagttcgt aagttaagta
    11761 ataaacagga aaatagtatg ttacta
//
LOCUS       DANJFZ010000081        11766 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.081, whole genome shotgun sequence.
ACCESSION   DANJFZ010000081 DANJFZ010000000
VERSION     DANJFZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.081"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(14..841)
                     /locus_tag="PWN45_001902"
     CDS             complement(14..841)
                     /locus_tag="PWN45_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0623668.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(844..963)
                     /locus_tag="PWN45_001903"
     CDS             complement(844..963)
                     /locus_tag="PWN45_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623669.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1075..2076
                     /locus_tag="PWN45_001904"
     CDS             1075..2076
                     /locus_tag="PWN45_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDL0623670.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2198..2662)
                     /locus_tag="PWN45_001905"
     CDS             complement(2198..2662)
                     /locus_tag="PWN45_001905"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL0623671.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="PWN45_001906"
     CDS             complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="PWN45_001906"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDL0623672.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3867..4499)
                     /locus_tag="PWN45_001907"
     CDS             complement(3867..4499)
                     /locus_tag="PWN45_001907"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL0623673.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="PWN45_001908"
     CDS             complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="PWN45_001908"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL0623674.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5676..5811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6030..7532)
                     /locus_tag="PWN45_001909"
     CDS             complement(6030..7532)
                     /locus_tag="PWN45_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL0623675.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            8055..8369
                     /locus_tag="PWN45_001910"
     CDS             8055..8369
                     /locus_tag="PWN45_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0623676.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            8369..9661
                     /gene="arsB"
                     /locus_tag="PWN45_001911"
     CDS             8369..9661
                     /gene="arsB"
                     /locus_tag="PWN45_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDL0623677.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            9679..10074
                     /gene="arsC"
                     /locus_tag="PWN45_001912"
     CDS             9679..10074
                     /gene="arsC"
                     /locus_tag="PWN45_001912"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDL0623678.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(10369..11223)
                     /locus_tag="PWN45_001913"
     CDS             complement(10369..11223)
                     /locus_tag="PWN45_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDL0623679.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(11499..11723)
                     /locus_tag="PWN45_001914"
     CDS             complement(11499..11723)
                     /locus_tag="PWN45_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623680.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT         3751 a         2075 c         1670 g         4270 t
ORIGIN      
        1 cctttttaga atattaaatt tctacaattt cgtcatcttc aacaataaag cccattgtat
       61 tgacgctgtt atttaagaaa gtcagaatat aacgcattac ttcatcacgt tctggctcat
      121 tgtgaacctc gtggtaaaaa ccttgccaag ctttaaaata taattcaggt gtttgatatt
      181 tttctttaaa ctcatcaatt gccctagtat caacaattaa atccttcgtt ccatacatta
      241 atagcgttgg cattggttga atgtcatgaa tatgagccat cgtatctttc atcgtctcat
      301 taattgtatt ataccaatga tacgttgctt tttttaacat taaaccatcg ttaactgttt
      361 cttcaacaat ttctaaatta cgtgttaaat cttttggttc tacaccaaca ttaaaacgtg
      421 tgtcttttga aattttacct atatttgaaa caagtttatc tttacgattt tttccattct
      481 tttgaagttc tagcatagga gaaattaaca tcatcccctc gattggcaat tctacttttt
      541 caagtaaatt taataaaatc aaaccgccaa gtcctacccc taatacataa gtaggaattt
      601 tatattcatt agctatcttt aaccagtcta gcaaactttc gtgatacgtt tgaaagtttt
      661 caatttgtcc tttattagct cttgaagttt gaccttgacc aggcaaatct cccataatca
      721 catgatagcc atttcttctt aacatcgtaa taacatatgc atatcttccc gtatgttcta
      781 atatattatg agcaataaca acgacgcctt tcgcatcatt ttcagcttcc cacttccaca
      841 ttattatact gccccttttt cattaatctt caataacata attatagcaa attcactatg
      901 tagatttcta tttatagtat tattgttgtc catattatta tatataaatg aaatcaacat
      961 caataatagt gtaattatac ataattattt ttgattgttt ttgatgaaaa cgctttctcg
     1021 aatatttttt tcatgctaaa cttattgtaa acacaagggt ttggaggagt agcaatggca
     1081 ctattaaaga atttttttat cggattatct aataatagtt ttttaaacaa cgcagcaaaa
     1141 aaagtgggcc cacgtttggg cgccaataaa gtcgttgccg gaaatacaat tccagagtta
     1201 attaatacaa tcgaatactt aaatgacaag aatatcgctg ttacggtaga caatttaggg
     1261 gaatttgtcg gtacagttga agaaagtaat catgctaaag aacaaatttt aacaattatg
     1321 gacgcgcttc atcaacatgg cgtaaaggca catatgtctg ttaaattgag tcagttaggt
     1381 gcagaattcg acttagaatt agcttaccaa aatttaagag agattttact taaagcaaat
     1441 acttacaaca atatgcatat aaatattgat actgaaaaat atgctagcct gcaacaaatt
     1501 gttcaagttt tagatcgctt aaaaggcgaa tttagaaatg ttggtactgt aattcaagca
     1561 tatttatacg atagccacga attagttgat aagtaccaag atttacgatt acgtttggtt
     1621 aaaggtgcat ataaagaaaa cgaatcaatt gcatttcaat ctaaggaaga cgtagatgca
     1681 aattacatca aaataattga acaacgtttg ttaaacgcac gcaatttcac ttcaattgca
     1741 acacatgacc atcgcatcat taatcatgta aaacaattta tgaaagaaaa tcacattgaa
     1801 aaagatcgta tggaattcca aatgctctat ggttttagat cagagttagc agaagaaatc
     1861 gcaaatgaag gctataattt cactatttat gtaccttatg gcgatgattg gtttgcgtat
     1921 tttatgagaa gattagcaga acgcccacaa aacctatctc ttgctgtaaa agaatttgtg
     1981 aaacctgctg gcttaaaacg tgttggcata attgcagctt taggagctac agttatgtta
     2041 ggtttaagta caattaaaaa attatgccgt aaatagagca agacataaac aataatttag
     2101 gagtctggaa caataatcaa tgttctaggc tcctaaatgt tatattggca gttctgaaac
     2161 atttataaat ctttgattat gaactcacaa cagtgctcta tgcttttata gattttaata
     2221 aattagccat ttcaattgca cttactgctg cttcagcacc tttattgcca gctttcgtac
     2281 ctgctctttc cacagcttgt tcaatacttt cagtcgttaa aataccaaat atgactggta
     2341 cattagtttg atcattcact ttagaaacac ctttcgcgac ttcattacaa acataatcat
     2401 aatgagacgt agcaccgcga attacgcatc ctaatgtaat tactgcatca taatttcctg
     2461 atgaggctaa ttttttagct actaaaggaa tttcaaacgc acctggcaca aatgctacat
     2521 caatattgtc ttcattaaca tcatgtcgaa tcaaagtatc ttttgcacct tcaagtaatc
     2581 ttccagtgat aaaatcatta aatcgactaa ctacgattgc aactttcaaa tcttttccaa
     2641 ttaatttacc ttcaaaattc atgttaaaat cctcctatat taaatgaccc atttttattt
     2701 ttttcgtttc catataatca tgattatgta ccgtttctgg tacgataact tcaattcttt
     2761 ctgcaatatc aatgccatat tgttttaatc cctcaaattt acttggatta ttacttaata
     2821 aattgatatg ttcgatgtta aaatatttta aaatctgtgc agcaatatga taatctcgca
     2881 aatcttcatc aaaacctaat gctaaatttg cagttactgt atcatatcct tgctcaatta
     2941 attcatatgc gcgtaatttg tttaacaatc ctatgccacg accttcttga ggtagataaa
     3001 taatcatgcc accatgttca ttgatatact tcatagacga ttcaagttga gcaccacaat
     3061 cacaacgttg actatggaaa atatcgcctg taaggcacgc agaatgtaag cgtacatttt
     3121 catgttgtcg aattgcacct tttgtcagta caactatctc ttcatctgtg tatgtcgctt
     3181 taaaaccata catatcaaat gttccgaaat ctgtaggcat tttcactttt gccttaaatt
     3241 caatttctgg ttctaatttt ttacgatatt caattaaatc atcaatcgta atcatcttta
     3301 attgatgttt ttctttaaac ttttgtaaat cttgtccttt cgccatcgtg ccgtcatcat
     3361 tcataatctc acaaatgaca ccagcgggct tggcaccagt aagtttagct aaatcaacag
     3421 ccgcttctgt gtgtccattt ctagctaata cgcctttatc ttgtgctact aatggaaata
     3481 aatgaccagg acgattaaaa tctttagctt cactactagg atcaatgagc tttttggcag
     3541 tcaatgtacg ttcataagca ctaattcctg ttgttgtatc tacatgatca atactcactg
     3601 taaattgcgt accaaagatg tcggagttat catcaaccat ttgtaccaaa tccaaacgtt
     3661 gtgcaatatc tttagacact ggtgcgcata ttaatcccct tgcttctttc gccataaaat
     3721 taatggtatt atcgttcatc cattcagtaa ccgctactaa atcaccttca ttttcacgat
     3781 tctcatcatc tactacaata attggttctc cattttttaa agccattaaa gcactgtcaa
     3841 tattatcgaa ttgcatgcta cccctcctaa aaaccaaatg ctcttaattt atctacagat
     3901 aattggtctt tatctttatt taaaatattt tcaacatatt taaacaaaac gtctgtttct
     3961 aaatgtactt tatctcctaa ttttttggat gataaaatcg ttgaacgcct cgtttctgga
     4021 ataagatgaa tgtcaaaact gttatcatgc ttatcaaata ccgttagact aacaccatcc
     4081 acagtaatag acccttgctt aactaactga ttattaatat gttggctaca ttgaatcgta
     4141 ataatttttg cattggctgt ttcatttatt tttgaaactg ttcctagttc atctacatga
     4201 ccgaggacaa aatgtccacc aaacctaccg ttaccactca tggcacgctc taaatttact
     4261 tctgattgtc gcttaacatc tgctaaatag gttttatttt cagtgccttt aattacttga
     4321 acagtaaaag atgtctgatt aaaatcaatc actgttaaac atgcaccatt aacactgatg
     4381 gaatcaccaa tatgcatatc tgccgtaatc ttatgtgctt caatttcaat cgtcctgact
     4441 gattgacgaa tttgaacact tttaacgaca cctatttctt caacgatgcc agtaaacatg
     4501 catcatcact tctttcgtaa agttaattta acattttgat ttaataactc ggaatgaaca
     4561 atttcaaatt ggttcgcatc tggtatctca atcacatcat ttgtttgata aaattgataa
     4621 tttccagatc cgccaattaa tttcggggca taatagagaa taaattcatc tatataatta
     4681 gattggagaa attctgaagt agtggttgga cctgcctcaa ctagcaaagt tccaactcct
     4741 cttttatata aattgtgaag aattgttgtt aaatcgcaag acttcaagta aataatttca
     4801 atatgtgttt gattggttgt taaatttgga ttttcagtat atatccaaat tggtgttgat
     4861 tcatcttgat aaatttgctg attaaaatga atattcccag acttagacaa tattactttt
     4921 atagggtttt ttccatcttg aatacgtgta gtatattgtg gatcatctaa ttcaactgta
     4981 cgtcttccag ttaacactgc gtcgtgtcga tgtcttaact tatagacatc ttgtttaacc
     5041 tctttgttag taatccattg actttgtcca ttatcattcg cttgtttacc atctaaactt
     5101 gcagatactt tcactgtaat ttgtggcagt tgctttgctt ttgctttaaa aaagtcttgg
     5161 tataattgtg atgcccgttc atcatcaacg cattcaacct caataccgtg agcccgtaac
     5221 gtctcatcac catgtgtgtc taacgaattg tcttttgttg cgtatactac ttttgctatc
     5281 ttacaatcaa ttattttgtt aacacagggt ggtgttgaac caaaatgact acatggctct
     5341 aacgtaatat aaatcgtcgc accttcagca ttttgttgtg ccatatcaag tgcttgaacc
     5401 tccgcatgct tgtcaccttt tctcaagtgt gcaccaatac caacaatcct accttcttta
     5461 actacaacag cgccaacggg tggattaaca cctgtttgac cttgtaccat atttgcaagt
     5521 tgaatcgcat aatccataaa ttgactcaaa tgatcacctc tataaacaaa aatcctcaca
     5581 tcatgaatta agatgcaagg agaaaaattt atcgttaaat aagcctattt gtacacattt
     5641 ttacaaatac gctacattat ctttgtcgat aattaacatt ctttctccca tccagacttt
     5701 aactgtcggc tctagaatct cactagatca gccactaata tgaaacatat tagcaggtcg
     5761 caggctttat ttactgccgg ttgggaattt caccctgccc cgaaagaatt atatatgaaa
     5821 ttgttataga ttatttgagt acgtagtatg tcaactacat ttaaaatgat actatatgtt
     5881 ttctgaaaaa acaattaatg acggttttaa tttaatataa tctgagtact ataggcatct
     5941 cattgatatg attcttacta acagacatta aaatcaaacc ttcaattcgt ctctatagag
     6001 cgttctcttt attatcttct agttacaaat tattgattgt cactgcgctg ttgttgctca
     6061 ttcgattcta aagcatcata taattgagat actgtatgcg caacttgttc tacaatcatt
     6121 ttcacaccgt ttcgtagttt attaacaccg tttgtcattt gacctatcgc aatcatattt
     6181 gttaatgttc caaaccttgg actaataact tgattggttt ccggaatgat ttgtatgcct
     6241 cccattgggt gtgcttgtac aatttgtcta ttttcaagat ttctaattaa ttgatcatct
     6301 tgatccaatt catttaaatg actttttgca cctgtcgcgt taatgacaac attatatatg
     6361 tctactgatt cttggttttt gtatgaaaaa taatacaact tgccatcatg tttcacatct
     6421 tctaaatctt ttttcaaaat taaagactta ttttctatta attcaataat tagttcagca
     6481 gttcttggag gcattggatt tgaatttaat tgaatcatct ttgagtattt ttgattaaat
     6541 tgatgttggt cttcaatact taagctattc catatccaat ttaaattctc tttcaaatgt
     6601 tcaatcatac tttggaaaat gcccatttct gttggacgcg ctaaatcata cttcaaatct
     6661 gcaatatgat ttcctgtacg tctatgtact aattttttaa aatcaatgtc ataatcagca
     6721 cattctttta aaaataaaga aactaaagta tcaagcggtg cattgccgaa atgatgtttt
     6781 ttaatgtcat ttaatttgtc tttagttaag tacttgaatg tcacgtctat cattgtacct
     6841 cttacacttg gtaaatgagc agaacgactc gtcatagtaa ttggtaattt tggatgatga
     6901 gcagcaacat aacggacaac atctaaactg gcaaggcctg taccaataat cgcaatatcg
     6961 tccagttcat ttacttcgtc taacgtatta tatgttggat aaggcgtagc gatatatcct
     7021 tttttaccct ttaagttata tggatcatgg taggcaaatg taccacatgt taaaaataca
     7081 taatcgtacg cttgccatga ttgtcctgaa tttgtagtac atatgtaata agttaaattc
     7141 gtttcatcga tattagaatt tgtataaatc tcttgaactt tattataatt agttgatata
     7201 tttggatatt ttttcgtgaa catagataaa taagatttca tataatgtcc gaatacaaat
     7261 ctcggtaaat atgcaggttc atcaaaatta aaatcagttt gttgtttata ccacttccaa
     7321 aattcagtct catcatctaa atttaaactc atctttttcg aaggcatatt aattagcagc
     7381 tcagaactat cattttgaaa tggtacgccc tgtcccatat ttactttatc atcgtataaa
     7441 tctatatcta attgattaaa cttcgggtgc ttaactaact ctctcaatac acttacacca
     7501 gcagttccca tgcctattat tgctacacgc atatatcatc catccttttg taattcaaaa
     7561 tattatattt aaactattac ctataattat agcaatttgg tatttccaat aaaatttatt
     7621 aaggtaagct attatttcac tttcacaacc attaaaccat ttaaaacttt tcatttaata
     7681 tcaaccttag tgtttgtgtt aaaatatttt taacgaggtg aattaaattg aaatttatag
     7741 caaagatatt aatctcatta gtaattctat ttatgatttt tagtcgtaaa aacaaagctt
     7801 cttaatcaat acccttgatt acaaaataaa ttcaactaag gtgattggcc atacactcaa
     7861 gatatggcta atcacctttt tcaatgcaga acaaagagta tattttgcaa aatcaatatc
     7921 tataatttat ctttaatacc cttttaatac taaaaccaaa tttaaattac atagtcatat
     7981 cgtgtaatat accaaaggaa catagacatt catctatata gatgataatc tatttaacgt
     8041 ctaggaggta tgttatgacg tataaagaac tagcaacatt tttaaaagtt ttatcagatt
     8101 caagcagatt agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt
     8161 tagcacattt tcaattctct caacctacac ttagctatca tatgaaagca ttagtaaaaa
     8221 ccaacttagt tacgacacga aaaatcggaa ataaacattt ataccagctc aatcataata
     8281 tttttgagtc cgtaattaat aacttgtcta aggttcatac ctctaaccaa cgatgtattt
     8341 gtcataacct taagactggt gaatgctaat gatgacaact ttagcgacac tcatttttct
     8401 agtaacttta ttatttgtat tatggcaacc taaaggccta gatattggca ttaccgcact
     8461 aactggtgcc tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt
     8521 aacaggtatt gtttggaatg ctactttgac ttttgtctca gtcattctta tttcattaat
     8581 attagataaa gttggattat tcgaatggtc agctattcac atgcttcatg cttcaaaagg
     8641 caatggttta aaaatgttcg tttatatcat attattgggt gccattgttg ctgcattttt
     8701 cgcaaatgat ggcgcagcgt taatcttaac gcctattgta ttagcgatgg ttaaaaatat
     8761 aggttttagt aagcgggcca tattcccttt tattattgcg agtggtttta tagctgacac
     8821 aacttcttta cctttaatcg tgagcaatct agtgaatatt atatctgctg attattttca
     8881 tgtaggattc gttcgatatt ttagtagaat gattatacct aatttattct cgcttttagc
     8941 aagtattata gtattgtggt tatattttag aaaagcgata cctaaaacgt ttgatgataa
     9001 taatataaag catcctaaag atgccattaa tgatttaaag ctatttaaaa tttcatggat
     9061 tgttctagtt atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc
     9121 aattttcact ggaatcattg cttttatttt tctaatgttg gctcgtaaat caaatgctgt
     9181 aaatattaag caagtcatta agggcgcacc ttggaatata gtattatttt caattggtat
     9241 gtatatcgtc gtattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt
     9301 agaatatatt tccaattacg gtctatttag cactattttg ggaatgggct tcatttcagc
     9361 gtttttatca tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg
     9421 tcaatcaaat gtccatggca tgttaaaaga aggcctaatt tatgcgaatg ttatcggttc
     9481 tgatttaggt ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt
     9541 cttaacacaa aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat
     9601 tacaattcca gtactattta taaccctcat agggttgtat ctaacactta tcatatttta
     9661 attaattagg agattgttat gactaaaaaa acaatttatt ttatatgtac aggcaactca
     9721 tgtcgaagtc aaatggctga aggttgggct aaacaaatct tagcggatga ttggaatgta
     9781 tattctgctg gtatcgaaac acacggtgtt aatcccaaag cgatagaagc tatgaaagaa
     9841 gtaggcattg atatatcaaa tcatacatca gatttaatcg ataataatat tattaaaaat
     9901 tcaaatttag ttgttacatt atgtagtgat gcagacgtaa attgcccttc tttaccaaca
     9961 aatgttaaga aagaacattg gggatttgat gatcctgcag gcaagccttg gtcagagttc
    10021 caacgtgtaa gagatgaaat taaaatcgca attgaaaatt tcaaatcacg atgagagtgg
    10081 gacagaaatg ataaaaaaac actaatgatt tattatgtag tggttcttta tcattagcta
    10141 taactaatgt gtacttaaaa ataggaatac atgagtaaaa ctcatgcata agaaatacta
    10201 atttctaaag aaaaagtaat tctttatcga tgtcctgccc cacttgcatt ggtgggatta
    10261 tcaatcaatg tgttttctct aatgcaaaaa gacgctttct atcaaaacaa gatagaaagc
    10321 gtctttttag tatgtccgag tcataaaaac aactcgtagc ttatcaaatt acttattcaa
    10381 atgtttactg tcatctttat acacaaagta tttgaagtat tttccttcag tcttcatgtt
    10441 cttataaaag tcagcgataa ttgttgcatt actttctgcc cacttaatat ctgtagcata
    10501 ttgatgttct cctggatttt ttggattcca tctcatacta tacaatgtat tttgatctgt
    10561 gcttgataag aagtgcttat gaatgaaatc agcaccgcct gaaatagctt tttcaggtgt
    10621 atcccaacca tgctttttag catattctgc acctgtttta attgggtctt tatcaagggc
    10681 tcctactcca tagaaattgt agtacttttt gccatcaatt tcgactccat tagctaattc
    10741 acttttaact gcgccagttt ctaataatgc atgtgaaatt aaataaactt cgttaacgtg
    10801 cttatcttta gcagctttta agaaatcatc cgtatgtttc aataacgttg gtctatctac
    10861 taacatacgt ttaattctat ttttatcaat cccttgatac tttgataaat ctaaaaattg
    10921 atatttttgc ttttcattat cgataaaagt accgctatcc attgcacttt taatttcagt
    10981 tgcagatgca tctctccatg catcattctt tttatttgat acctgttgac tcgtataatt
    11041 atttatttgt ttctttgctg catcgtttaa tgtaacattt aacttttcaa tcttaatgtc
    11101 ggatttaaca tgtttgaaaa atatctgatc ggatatcatt gagaaaaata aaaatgagac
    11161 aacagcaaat atgacaacaa gtcctattat tccaaaaata gaacctttct tgtgtttatt
    11221 catatccaca cctcttaggt cattgttgtt atacattcag tttgactacg ataataaagt
    11281 ttaacattat taaaaccgtt taacaatttt atctatgcat tgttacatat tcataaataa
    11341 acagtaatgt aaatgaaacg ttttaaacca tttatcaaca ttactttacg ttgatctacg
    11401 aatcataaat gttttatctt tttgtataac ctttgaaaac ctttatatca ataatcttat
    11461 tattcacgta ttaaaaaaca aaaaattctt ttgttcgttt acttagtttc ttttttataa
    11521 tccatcataa taaaacttgc tgcatttgat ctatttttat aatagtactg atttaaatca
    11581 atcgtgcctt caatttgacc atcacgatac atcatttcat tagtgaagtt atttatcatc
    11641 acgaaatcag cgcgatctct tataacatct aaatcgtcgt ttcgtgcaaa aaaatgttct
    11701 aaatttaaat gtgcgtaatc cattgttacc tcccgattac aaattttgtt ctatctacta
    11761 tttagc
//
LOCUS       DANJFZ010000082        11724 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.082, whole genome shotgun sequence.
ACCESSION   DANJFZ010000082 DANJFZ010000000
VERSION     DANJFZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.082"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            625..807
                     /locus_tag="PWN45_001915"
     CDS             625..807
                     /locus_tag="PWN45_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623681.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(789..1028)
                     /locus_tag="PWN45_001916"
     CDS             complement(789..1028)
                     /locus_tag="PWN45_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623682.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1261..2925
                     /gene="alsS"
                     /locus_tag="PWN45_001917"
     CDS             1261..2925
                     /gene="alsS"
                     /locus_tag="PWN45_001917"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL0623683.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2962..3666
                     /gene="budA"
                     /locus_tag="PWN45_001918"
     CDS             2962..3666
                     /gene="budA"
                     /locus_tag="PWN45_001918"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL0623684.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4030..4455)
                     /locus_tag="PWN45_001919"
     CDS             complement(4030..4455)
                     /locus_tag="PWN45_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDL0623685.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            4749..5564
                     /locus_tag="PWN45_001920"
     CDS             4749..5564
                     /locus_tag="PWN45_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDL0623686.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            5775..6629
                     /locus_tag="PWN45_001921"
     CDS             5775..6629
                     /locus_tag="PWN45_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDL0623687.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(6689..9112)
                     /gene="hysA"
                     /locus_tag="PWN45_001922"
     CDS             complement(6689..9112)
                     /gene="hysA"
                     /locus_tag="PWN45_001922"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HDL0623688.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(9109..9276)
                     /locus_tag="PWN45_001923"
     CDS             complement(9109..9276)
                     /locus_tag="PWN45_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623689.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            9520..9936
                     /locus_tag="PWN45_001924"
     CDS             9520..9936
                     /locus_tag="PWN45_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0623690.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            10006..10854
                     /locus_tag="PWN45_001925"
     CDS             10006..10854
                     /locus_tag="PWN45_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0623691.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            11074..11199
                     /locus_tag="PWN45_001926"
     CDS             11074..11199
                     /locus_tag="PWN45_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623692.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            11461..11724
                     /locus_tag="PWN45_001927"
                     /pseudo
     CDS             11461..11724
                     /locus_tag="PWN45_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4136 a         1683 c         1974 g         3931 t
ORIGIN      
        1 atattttaaa tatggataaa gttgatatag aaaatttttt caaaagtata gaagtcaaat
       61 acagtaaata agatcaatta ttgaatccac cgaccagcag atttttttaa aaaattaata
      121 ccccgttaat accctttgct tcaatttgat gaaaatcaat gaaattcaaa attgaagaaa
      181 tcctttaata tcaaggtgta cgacagtcta tataatcatg cgaaattcta aaattttctg
      241 aacgtaaaaa aacaaattct taaagcagct cgtcgttcac ctcaattctc aaaacgttaa
      301 ttgtcggacg atatatatac aaaacacctc gatgttatgt cgaggtgttt ttttgcgttt
      361 gtgtggggaa tatggaatag agtataaatg aattaatgtc tcaggtatag aattaattca
      421 actatgaatt attgatttac acgtataatt tgtacatgct tacacaatca aaagtgtaca
      481 ttattaaatt gtcatttagc ttgcggagac cgtacgatag tgttctaata atagatattc
      541 aagggaaaga gctatcggat gataaccaga tgataactca agaaccatga tttaagagtg
      601 ttatcaaaat aaggagtgac ttaattgaaa gattctatat tttggaagaa agcttttatt
      661 cctgtttatt ttattgttgc gatgctggtg tttctacttt ttaggtttta tattaaaaca
      721 gataactttt ctatatattt aatgagtatc ttcttaattt gtttaggaac tgcttctatc
      781 atttataact ataaaaccaa tcgataaata taagtatgaa atgtataagt cacagttaga
      841 tctaagtctt gctgtggctt tttatatctc cataaaacga caaattcaag cccgacataa
      901 aacagcattt tcagcccacc ataaaacgac aatttcagcc cgccattgac taaacaccac
      961 atcccaaaaa tatcgtaaca atcctctaca tcaatcaatc caacatccct cataatcaca
     1021 acgcacaaaa tctattcatg catttttgga atacttagta ttacaaataa cgatttttat
     1081 tcatctaaca aaggttatat aatgtactga aggcaatttt tatgtattac aaatctaatc
     1141 gtacatgtaa aattttgata aacatcatta attttgcgta actatcatta gattacaaat
     1201 cacaaagtaa ttacatgtaa tacacatcta tacatcacat ttgaagggaa atgaatataa
     1261 atgactgata aaaagtacac tgcagccgat atggttattg atactttgaa aaataatggg
     1321 gtagaatatg tttttggtat tccgggtgca aagattgact atctatttaa tgctttaatt
     1381 gatgatggtc ctgaacttat tgtcactcgt catgaacaaa acgccgcaat gatggcgcaa
     1441 ggtattggaa gattaacagg caaaccgggt gtagtacttg ttacaagtgg tcctggtgta
     1501 agtaatttaa ctactggatt attaaccgca acatctgaag gggatcctgt attagcgtta
     1561 ggtggccaag tgaaacgtaa tgatttatta cgattaacgc atcaaagtat tgataatgct
     1621 gcgctattaa aatattcatc aaaatatagt gaagaagtac aagatcctga atcattatca
     1681 gaagttatga caaatgcaat tcgaattgct acttcaggaa aaaatggcgc aagttttatt
     1741 agtattccgc aagacgttat ttcttcacca gttgaatcta aagctatatc actttgccaa
     1801 aaaccaaatt taggagtacc gagtgaacaa gatattaatg atgtcattga agcgattaaa
     1861 aatgcatcat ttcctgtttt attagctggt atgagaagtt caagtgcaga agaaacaaat
     1921 gccattcgca aattagttga gcgcacgaat ttaccagttg tagaaacatt ccaaggtgca
     1981 ggtgtaatta gtcgtgaatt agaaaatcat ttcttcggtc gtgtgggctt attccgcaat
     2041 caagttggtg atgaattatt acgtaaaagt gatttagttg ttacaatcgg ttatgatcca
     2101 attgaatacg aagctagtaa ctggaataaa gaattagaaa cacaaattat caatattgac
     2161 gaagttcaag ctgaaattac taattatatg caaccgaaaa aagagttgat tggtaatatt
     2221 gctaaaacga ttgaaatgat ttctgaaaaa gtggatgagc catttataaa tcaacaacat
     2281 ttagacgaat tagaacaatt aagaacacat attgatgaag aaactggtat taaagcgacg
     2341 catgaagaag gaattctaca tccagtggaa attattgaat ctatgcaaaa ggtattaact
     2401 gatgatacta ctgtaacagt tgatgttgga agtcactata tttggatggc acgtaatttc
     2461 agaagttaca atccaagaca tttattattt agcaatggta tgcaaacgct tggtgtagca
     2521 ttaccgtggg caatttcagc tgcacttgtg cgccctaata cgcaagttgt gtccgttgct
     2581 ggcgatggtg gctttttatt ttcatcacaa gatttagaaa cggccgtacg taaaaattta
     2641 aatatcatcc agcttatttg gaatgatgga aaatataaca tggttgaatt ccaagaagaa
     2701 atgaaatata aacgttcgtc aggtgtagac ttcggtcctg tagattttgt aaaatatgca
     2761 gaatcatttg gcgcgaaagg tttacgagtt actaatcaag aagaattaga agcggcaatt
     2821 aaagagggct atgaaacaga tggtccagta ttaattgata tacctgtaaa ttacaaagat
     2881 aatatcaaac tttcaacaaa tatgttacct gacgtattta actaaaataa agataaatgt
     2941 taaagaggag tgggagattt tatgactaat gttttatacc aacatggtac attaggcaca
     3001 ttaatggcag gattattaaa aggaactgca tcaataaatg aattattgca acatggtgac
     3061 ttaggtatcg ctacactaac aggttcaaac ggtgaggtaa tctttttaga tggaaaagct
     3121 taccatgcaa atgaacataa agaatttgta gaattaaaag gtgacgagtt aacaccatat
     3181 gcaactgtaa ctaaatttgt agcagataca agctatgaaa cgaaagataa atcttcagaa
     3241 gcagtttttg cagaaattaa ggaaaagatg ttgagtgaaa atttattttc agcagtaaaa
     3301 atttcaggct tatttaaaaa aatgcatgta cgtatgatgc cggctcaaga accaccttat
     3361 acacgtttaa tcgattcagc tagaagacaa cctgaacaaa ctgaaacgta tgtcaaaggt
     3421 tcagtcgttg gtttctttac accagaatta ttccatggta tcggatcagc aggatttcat
     3481 gtacactttg cgaatgatga tcgtaacttt ggtggacatg tcttagattt tgaagtagaa
     3541 gatgttaaag tagaaatcca aaatatagaa acatttgaac agcattttcc aattcaagat
     3601 aaagatttca ctaaagcaaa tattgactat aaagatattg cagacgaaat tagagaagct
     3661 gaataatgaa tccagaaata taatgacggt ttatgaaaat tgacttcata atgcgcgatt
     3721 tagaaatgat agtttgtaaa tatgattaac catgactaca atagaacaaa tatatttata
     3781 attacgtcta agtaataaaa taaatccctt cactattagc agtagtgagg ggatttatta
     3841 ggttccagat atttgagatt tgctgttatg tttagattat aaattgtggt acacactcat
     3901 ataaaattta ctattgtata ggccaatctg ttactacgag aagcaaacaa caataattta
     3961 caagttcaat aactaaaaag acaaacgcca atttttcagc gcttgcccta taaaactatt
     4021 ttcaaattat tatttaacta caatttgtat ctgtttgatt gtacttggat caattgaatt
     4081 tttagcgtcg tcatttttct ttagattcac tactttttta ttaccatcat tcaaagtaag
     4141 cgtataagtt gctgtttggg cattattaat tttttctgtt gtaacaccac gttgagaagc
     4201 taattcattt tttactttac tgtcaatttc ttgataaaga acatttttat tttctgggaa
     4261 gataaagtaa gttcgatgta atgcagtaat accatctact gaaattgtgt aaggaacagt
     4321 gtgataacca tccacagtca tttctttata gccgttatta ctatctgcag atgcttcgtg
     4381 actcggtaat gcagctccga ccgttgataa taatcccaat gctaaagtgg cagtaacaaa
     4441 tgattttagt ttcatatctc aatctccttt tcgggttgta tgatattaca ctatttgtat
     4501 aacacgtttc ataaagttta aaacatactt tttgcatttt ttatatttta atttttagta
     4561 atagttttta acgagggata tgactcgtgc attttgaaaa gtggtaagca atgtttttag
     4621 tttataggga ttgattagtt gtatataata atcatgaaaa gcatatagtc gatcttatac
     4681 ttttaattga gacttatagt gtatttaagt tatagataaa ttcattaaaa tattatgggg
     4741 gaatgagtat gtcaaagcgt ctattattgt ttgattttga tgaaacatat tttaaacata
     4801 atacaaatga agaagattta agtcatttaa gagaaatgga aaaattgttg gaaaaactaa
     4861 ctaataataa tgaagtgatc actgctgtat tgacaggaag tacatttcaa agtgtcatgg
     4921 ataaaatgga tcaagttaat atgacgttta aaccattaca tatcttttct gatttgagtt
     4981 ctaaaatgtt cacttggaat aatggcgaat atgttgaatc agaaacatat aaaaagaaag
     5041 tcttgagcga accattttta tttgaagata ttgaagatat attacgtcat atttctgcgc
     5101 aatataatgt cgaatttatt ccacaaagag catttgaagg taatgaaaca cattataatt
     5161 tttattttca ttcaacaggt aatcacaaca atgatagtcg tatcctagaa gcacttgtca
     5221 gatacgcaaa tgaccaaaac tatacagcga gatttagtcg aagcaatcca ttagctggtg
     5281 atcctgaaaa tgcgtatgat attgacttca caccgtcaaa tgctggaaaa ttatatgcta
     5341 ctcaatttct gatgaggaaa tataatatac cagtaaaatc aatattaggt tttggagata
     5401 gcggcaatga cgaagcatat ttaagctatc ttgaacatgc atatttgatg tcgaatagta
     5461 gagatgaagc attaaagcag aaatttagat taacaaaata tccatattat caagggataa
     5521 cactgcatgt taaagaattt gttgaaggta agtatgatta ttgattagtt gagtgatatg
     5581 tatacttttt aagtgcaaat attaatattt aagggatgat tagaaaatat ggtgaatcga
     5641 ctacacttta gctttaaaaa attatttata tgttgattta tccaattaag tgtagacgat
     5701 tcaggtagcg caaaaatgac gcaggaactg gagaaagaat tgataccaaa attaatataa
     5761 taggttaagt atatatgaat aaaacaaact ttggggctta tttaaataaa agtaagagca
     5821 ggaaaatata taagattttt gatcaaaatt tgaagcgaga gtttagttat tggcagttta
     5881 ggaaactttt caaatcttat acaaggcagt ctgatgtaca tgaattaaaa gtgtgtactc
     5941 aaaatgatgg tactattgag caaatatgga tagatgaagc aaatcaattt ggagtgtcag
     6001 tgacttggaa agataatagt attatcggat ttgttatgcg tccgttaatt aattatgatg
     6061 cgattaagaa tgaaacgact ttaaaatacg cagtgccgac aacagattgc tatgagattt
     6121 tttggggtgg agataatgaa ttatataatt atcactattt atacccacat caaaggtatg
     6181 ctattgattt agttattaaa gaaaatcata aaacatataa gaatcaagga aaagtaaata
     6241 ctgattattt ttgttttgga aaagatatca ttgcgccagc aaatggtaca gttgaaaaag
     6301 tagtgaatgg ggttcaagac aacagtattg gaagtacgaa tgaatcacag tttttaggaa
     6361 attatattgt aattaagcac gcagaaaatg agtatagctt aatagctcat ttacaccaat
     6421 attcaatcat tgtgaatgag gggcaaaatg ttaaatatgg tgatatcatt gggaaggttg
     6481 ggaattctgg caattctact gaacctcata tacattttca agtaatgaat gataagaata
     6541 ttgaagcatg tacatcttta aaaattcgat ttataaataa tcgagaactt atcaaagggg
     6601 atgtggtctg cggattacaa gctgaatgat ggcgatactt ataaaatctc gacactataa
     6661 aaatggtata gtgtcgagat tttcttgctt atttagttaa ttcaaagtgc acgccggatt
     6721 cattagaagt cgacgtattt ttgtttgtaa tagagtaacc ggtcattgaa attttagatt
     6781 caatatctga agcggaattt gtagattcag gattatagaa gctacattca taagtgttat
     6841 catctttctt tttaagtata aacatacctt tggctttaac ttcgacttta gtgttgttaa
     6901 tgtcaaaagt ttgagtgcta ttactataat taacaccagc ccaaaccgat tcattatctt
     6961 tcacaacgtg gaagtcatct tcttgcttaa cgacagttac ttcatctttt tttgtcttaa
     7021 agacatcttt agataagcct ggatacaaca catatccata tttattgtca gaattagaat
     7081 gcttttgagt tacttcataa tactcatcag ttttttgtgt atccttttga cttttattta
     7141 tttctttcca cttaccagta tgactttctt tttttacagt tattttcggt ttgtttaaaa
     7201 aatgataacc gatgttcttt ttggtatctg tggactctaa aaagactgaa ttgttttcct
     7261 gattatcaga attggttgtt tgtttatcgt ctgtatataa cgtataccca ttcgctttgc
     7321 gattttcaat cgttgtaact ggattctttg atgaatcagt acttttaatg ccagttccta
     7381 agaagacaat tttatcgttt aatatgaaat atgatttttt ggcagttaaa gttttgtcct
     7441 gattttcaaa atccattccg atactagcat gttggtcatc aacttttgtt ccgccaacaa
     7501 aagttttact cgacttttta tcatccgtat cttttaatat ttcattgtct aaagttgttg
     7561 tacctgataa acgtttcata tcggctgtca cccagaagtt atcatgatag tgtttgacat
     7621 cgctgttata taaataagac attccagcac cagtgtgcca accttttaaa ttctctccgt
     7681 tgatactttc atagcgtgct acgtttttcg acgtcatact taaaccaaat gcaaagtcta
     7741 agtctttgtt atgataggtg acacgatcca tgtcattata tattttaagt tgttgtgtta
     7801 atccgttttt agaaatactg ttatctgtca ttaaagactt cattttatct atgtccgaat
     7861 atgaatttaa ataatcattt tgtttataac ttgaatctga ctctactgaa gatttgacaa
     7921 tctttttata cttagctttt gttgaatcat ccatggcatc acttaatctc aacaatgatt
     7981 tcattactgt tgcagatgct gagtgactgg tttcattttc acgactgata gctctacctc
     8041 gtgataaatc catcatttca cctttataaa tgagtggcat aaatccgtcg tcaatccatg
     8101 actttaaggt tgtatcattt tgggttttat cattaaaagg tgtttctttt atcatcggca
     8161 tcatttgaga aatcccctct aagagtacaa cgccataagc accagtgtat ggaacgtctt
     8221 gatgatcaat gtaagagcca tctttataaa atccattacg ttctttacca gtggcagaat
     8281 cttgaacgta agtgaagact ttattaaatg aatctataga ctttttcatc atatctttat
     8341 cttcttcgat aatacattct aaaagtttca ccttagaaat gtctactaga tttccgcctt
     8401 tagcaagttc agcttttcct acagaagata atattttgtc actatctggg gcgaaagttt
     8461 taataggggc agtgaatttt ttcttttctt catttgaaaa ttgatcattc agcaatataa
     8521 gcgtatttgt taatgattta ggtgttccaa tttcataatc ccaccaattt aagttggtat
     8581 tcttgccagt tgttttagta aaattctcac ttaattcttt tactttttta tctggttctt
     8641 ttccatatgc atttttatgc agccactcaa gggcatcttt cactttcttc ttattttcgt
     8701 cagtatttaa agtggtttta ggattcctca tcgcttctgc gattttctca atattacgat
     8761 aggtacgagt catatgagaa gaattagttt caagggtttc cgctcctgac cacaagtatt
     8821 tcctaccact ttcagttttc atttccttga gtaaattcgt cgcctctttc tctgtagcat
     8881 caaacttctt cttcatatct ggattattct catcatactt atcataacca tagttaacgt
     8941 ccagccatgt gttcctcaat ttttcataat ctggcgtttg aacattcgta tcagccacag
     9001 cgatttgatg tttatcaaca cttctgaatt caccaccatt caaagtaatc acaccagcca
     9061 ttaataacgt aatggtggat aatttttgcc atttctttat tctatatgtc attgacatgt
     9121 ctcctttttg tgttgcgcgt gcgcaatgaa tattatgatt aaataatgat tcaatttttc
     9181 aaaattcgtt aacgtataca aatgactgtc tactgtcaaa caatccacaa agaatgttga
     9241 tgcatataaa caatcgatca cccaaatttt ccgcatcacc caaaaattgc aactaaaata
     9301 attttcaaaa aattaaatat tatcaatctt agtgattata tcattttaaa tatttaagtt
     9361 atacaatttt taaagtaatt aaaaacgatg aaatgtaaaa tgttaaaaaa gagacaaatt
     9421 taaatttgag taataaaata agaagtattc aaacgaagat gacaatatat aaagcaacat
     9481 tactttagac gaccaaacat aactgaaagg atagtcgcaa tgaaaactaa agaagtcgta
     9541 gcgctcatga atatatctca agacacttta agatattatg aaaaggttgg tgtgattcca
     9601 ccagttaatc gagatgaaaa tggatataga atatataatg atagtgattt aaattggatt
     9661 tatttagtga aaaatttgcg aaatgcaggc gtcagtattg aatcgcttat cgaattttgc
     9721 aggttagcgc agcttcctaa aaatgaaaat attcaagcac agcaaaagca aattttaaat
     9781 aagcaactcg aagaattaaa tgaaaactta aagacaattc atgatgtgag agatttacta
     9841 caatataaaa ttgataatta tgataatcat attgctaaaa tcaatgctag tgataattat
     9901 gatgacaatg ttgaacgtct ttgggagaga aagtaaacct atggcttgct atagagttcg
     9961 ctccatggtt tatagtggac atacaagata aaggaggttc attttatgaa tcatattgaa
    10021 ataagtaaag atgtaaagat accagtatta ggtttcggag tatttcaaat tcctcaagaa
    10081 caaacagcag aagcggttaa agaagcaatt aaagcgggtt atagacatat tgatacagca
    10141 caaagttatt taaatgaaac tgaagtaggt caaggtattg aagcttcagg tattgataga
    10201 agtgaactat tcattacgac taaagtatgg attgaaaatg ttaactatga agatactatc
    10261 aaatcaatcg aacgatcatt acaacgatta aatttggact atcttgatct agtgcttatt
    10321 caccaacctt acaatgacgt atatggctca tggcgtgcat tagaagagtt aaaagaaaat
    10381 ggtaaaatta aagcaatcgg tgtatctaac ttcggcgttg atcgaattgt agatttaggt
    10441 attcacaatc aaattcaacc acaagtgaac caaatagaaa ttaatccatt ccatcaacaa
    10501 gaagaacaag ttgcagcact tcaacaagaa aatgttgtgg tagaagcttg ggcaccattt
    10561 gcagaaggta aaaatcaatt attccaaaat caattgttgc aggccattgc agataaatac
    10621 aacaaatcaa ttgcacaagt tattttacgc tggttagtag aacgtgacat tgttgtactc
    10681 gcaaaatcag tgaatccaga acgtatggct caaaacttag atatatttga ttttgaactt
    10741 acagaagaag ataaacaaca aattgcgaca ttagaagaaa gtaatagcca atttttctca
    10801 catgctgatc cagagatgat taaagcactt acaagcagag aattagatgt ttaatcataa
    10861 tataacaaag taaatcattt acattacact aacctgaaac ttggatgtta aagtctatgt
    10921 ttcaggttat tttttactct taaaaagtga aaggagcaag caaataagtt attatatatt
    10981 tttatattat gtaataacgt aactaaggtt ataattagat aattaatatg tcttggaatt
    11041 aattatttaa taagttgtat agcaggtggc gatatgagat atatacactt ttctattatt
    11101 acattaatta caggtatcat tatgcatatt acaatgtact ttgtaccatt tgaatctcgg
    11161 aaaatgccac tatttatgac gataattttt acaatctaaa agcagtaaag actcataata
    11221 taaccaaaaa cattaataaa agtttgttaa atgataaggg gatgagtatt ggtgaattta
    11281 ctcaaaaggt ttaagggaaa aatcctacct ggagagattc aaaaactaaa tggactattt
    11341 ccaaaaataa agggcaatca catggtggtt cttattggaa gttaataaat aacaaaggaa
    11401 aaagaatagc ttctttaact aaagaaggaa aaatcttaag ggaataaggt gttgtaaatt
    11461 atgtgctttg atataaataa tttggatata aaaaagttga attttagaaa ggtaaaaaac
    11521 gcaattcatt taagtttaag tggcgataaa taccagtttt tggaggacgg taaaactatc
    11581 aataatactt atttttagct gtatatgata atgcaataaa catttttgaa gatttgtttt
    11641 cgccttctga ttctataaat ttggttcatg tagtatatgt ttatagtgaa ccttataaga
    11701 aaacaaatgt tataaaaaaa ttta
//
LOCUS       DANJFZ010000083        11648 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.083, whole genome shotgun sequence.
ACCESSION   DANJFZ010000083 DANJFZ010000000
VERSION     DANJFZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.083"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(50..910)
                     /locus_tag="PWN45_001928"
     CDS             complement(50..910)
                     /locus_tag="PWN45_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0623693.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            1023..1573
                     /locus_tag="PWN45_001929"
                     /pseudo
     CDS             1023..1573
                     /locus_tag="PWN45_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1566..2786
                     /locus_tag="PWN45_001930"
     CDS             1566..2786
                     /locus_tag="PWN45_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623694.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(2858..3154)
                     /locus_tag="PWN45_001931"
     CDS             complement(2858..3154)
                     /locus_tag="PWN45_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL0623695.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(3203..3406)
                     /locus_tag="PWN45_001932"
     CDS             complement(3203..3406)
                     /locus_tag="PWN45_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623696.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3715..4701
                     /locus_tag="PWN45_001933"
     CDS             3715..4701
                     /locus_tag="PWN45_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL0623697.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            5000..5101
                     /locus_tag="PWN45_001934"
     CDS             5000..5101
                     /locus_tag="PWN45_001934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0623698.1"
                     /translation="MSTRGLNIWQEMEIGNVSHKVAKRAQIPLIIFK"
     gene            complement(5368..6561)
                     /gene="sqr"
                     /locus_tag="PWN45_001935"
                     /pseudo
     CDS             complement(5368..6561)
                     /gene="sqr"
                     /locus_tag="PWN45_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(6580..7914)
                     /gene="cstB"
                     /locus_tag="PWN45_001936"
     CDS             complement(6580..7914)
                     /gene="cstB"
                     /locus_tag="PWN45_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDL0623699.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(7945..9012)
                     /gene="cstA"
                     /locus_tag="PWN45_001937"
     CDS             complement(7945..9012)
                     /gene="cstA"
                     /locus_tag="PWN45_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDL0623700.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            9149..9409
                     /gene="cstR"
                     /locus_tag="PWN45_001938"
     CDS             9149..9409
                     /gene="cstR"
                     /locus_tag="PWN45_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDL0623701.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            9409..10164
                     /locus_tag="PWN45_001939"
     CDS             9409..10164
                     /locus_tag="PWN45_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL0623702.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            10262..10363
                     /locus_tag="PWN45_001940"
     CDS             10262..10363
                     /locus_tag="PWN45_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623703.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            10379..10831
                     /locus_tag="PWN45_001941"
                     /pseudo
     CDS             10379..10831
                     /locus_tag="PWN45_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(10925..>11648)
                     /locus_tag="PWN45_001942"
     CDS             complement(10925..>11648)
                     /locus_tag="PWN45_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDL0623704.1"
                     /translation="MLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEI
                     EIRYNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELA
                     DLYNIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENI
                     FNDIQRSTNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWS
                     LYLKDQRINPIIYMETPTEDRN"
BASE COUNT         3819 a         1911 c         1716 g         4202 t
ORIGIN      
        1 taaattatta tctttgagtt tcaccataac tacacgtttt caccaataat tataaaaaaa
       61 catcttttac aaaagattca agtacttgct tattcgattc aggattaatt atcaatgttg
      121 tttctaaatt cggaatatcg acatctacgt ctatttgaaa caaactttta ttttctaatt
      181 catctgcaac aataaattcc ggcaatagcg caatcccacc tgaatttttc aagtgttttt
      241 ttaatgactc aattcctaga aattcaatca tttgttttgt tgaaaagcta tatttatata
      301 aaatcatttc taactgttct ctgtaactac accctttttc agtaacaagt aatgtatggt
      361 tttgaatatc gtttaatttg aaatctttca tctctttgtt agtaacaaat attaaacgtt
      421 cttcagttat aggaatagca gttaagttac tattaataat cttttcgtct gtgataaggc
      481 taatatccga tttgaaatta gcaatttctt ctttaatata gaaattagaa tgtgcggatt
      541 taaacttcaa gtttacatat gggtgttcag ctaaccattc actaatctta ttatagtagc
      601 gatacaaata ctgactttct tgcatgttaa tacttatatt taccttctta ttgtaaagtc
      661 cttttatttc tctctcgtat tcgctattac tatctaaaat cactttagcg tatttaacgt
      721 atgctaagcc ttcttccgtt agttccatac catatttttt tctataaaat aatttaacat
      781 ctaattgcct ttctaatttt tcaatatgtg tagatacagt tgactgtgaa tagttaagta
      841 actctgatgc tttagtaagg ttctttgttt cactagttac taaaaatgat tttaaaatgt
      901 ttaagttcaa agtagtacct cctttagggt tcccttttat cgaatcctga attctaagaa
      961 ttctgattta ataataatat attaaatatt attataaagg taaatcgaaa ggagttatga
     1021 ctatgattac ttgtttattt ggaagtagta gaaaggtggc aattcagcta tcgcagtaga
     1081 gaatctattg aagaatttaa aagtcaactt tgtgaattta tatcaaagca acattgaaaa
     1141 ggttacagat aatcgtcata gcaaaggtgc caactttcat aatgatgatt acgaacaaat
     1201 tttaatcaaa gttttagaat cagatattat tattttttca acaccactct attggtactc
     1261 catgagtgcg tctttaaaac tcttcattga tagatggacc gagtcattgc gagatacgca
     1321 aatagacaac ttcaaagaaa tcatgtcaca aaaaaagtat ttgattttaa tcataggtgg
     1381 cgacaatccg cataccaaag cacaaccttt agtcaaccaa ttcaaattaa tatttgagtt
     1441 tatgaatatt actaactata gatttttgat cggcgaaggc aataaaccat tcgatatttt
     1501 aaacgactct caatttatag aagagctcgc aaatataaat cttgcattga aaaaaggtga
     1561 tatttatgac tgatttgttg aaaattaaaa acttcagatt gttcttttta gcagaaataa
     1621 tttcagcatt cggagttgga attagtacag ttggggctaa ttggtattta atcgacaaaa
     1681 ctaacgactc ccaactatta ggtattatgt tagctttaaa tgtattatcc ggtttcttag
     1741 cttctcctat aataggtgga ctagccgata aatataatag acgaaatatt attttaatca
     1801 cctatcttct tcaggtaata ttatatttac tcattgtcat agcactcgta atgataggat
     1861 ttgagaccta tttagtgatt ggatttgcga ttgttaatgg gataggttgg actacgtata
     1921 tggcaacatc tagaagttta gttaaacaga tattaaaacc agatcaatat acagatgcaa
     1981 attctctatt agagattagt ttgcaaacag gtatgtttat tgcaggtgga ttatcaggaa
     2041 tattatataa aataaatgga ttcactctaa ttatagcgat gactataatg atgtttctaa
     2101 tcagcatttt tatgttattt agattgcatg tagataaacc aactcattca gaggaagaat
     2161 caacaaatag cttattgcaa gaatatttat taggatggaa atttttaaaa gacaacatga
     2221 tgatatttat ttttggagtt atttcaatca taccaatggt gtttacaatg atctttaaca
     2281 tatcattacc aggatatgtt tataatgttt taaaactttc gtctgtacaa tttggttttt
     2341 cagatatgtt atatggcatt ggaggattat gtgcaggttt aatttcagct attctttcga
     2401 agaaaatctc aactaaagta ttgatatttt tgttatattt catattagtc ataaattcag
     2461 cactatttat ttggataaac tcagcatttt acttattcat aggatcattt atactaggct
     2521 actcaatttc atcaatcaga atttatatga atacagctat aatgaacact gtttcagata
     2581 aatatgtcgg tcgctcattt acgatatgga cgtcaatttc attgttactg caaagtttaa
     2641 ttgctccatt tttaggaaga tggattaatg aaattaatga taaattcggt ttctatatta
     2701 tactcatttt atccttactc atatttgtta cactgctgct tgttaacaaa acaggcaaaa
     2761 taaaatatgc acataaagaa gagtgaccgt cactcttctt taacaagcga ccatttatcg
     2821 atgggcttag ttctctctgc acccacactt cactacttca ctttttcaaa tcatttttta
     2881 tggtcttaaa taaatcagtg agatttgttg cttcggtaaa gtctagaatt aatatcattt
     2941 ctttagaacc tggatatggc gaaactaatg tattatcttg caatttctgc tgggcacttt
     3001 tagtcgcctt gaccaataat ctattatcat acaaaccacc tataaccacg ccatcataat
     3061 aaataatata ttctcccatc atctttcttc tcttaaccgc gtttgaattc acatgattta
     3121 aaaataaatc atgtacatct ttcttagtcg ccattgtaat cgctccttca gttttatgtt
     3181 taatcacatt cgtattaatg attcatttcg tgttgctctt aattttatct ataattatat
     3241 gcgtagttaa aatcaaacct atggaaaaga aaataatgat aacaatgtta agaaatatag
     3301 ttataaaatt atagtttgga aggtatgcga atagcagaaa aataggtatc gcaaaaaata
     3361 aatcccacca acctaaactt tttaaagaat gctttaaacc ttccataata tcacctttat
     3421 aaatttgtct ttgttataag ataactaaaa aatcgcttta ctgtaaaagt agccaaagaa
     3481 aattctgaat catattcata agtagtgtat cattaataat gaacaattta atactataat
     3541 ccttgatctt tgtattgatc aacttaccac aacatttatt ttagactact cttagacttc
     3601 cctttcaaat ggttgcatct attgaaattc cttttgtata agttaggctt ttgtggtaat
     3661 atcatcatgc ataaaaaatc gagatactaa ttataaagag ggtataaata tattatgaaa
     3721 gaaaattttt ggagtgaatt accacgtcca ttttttattt tggcgccaat ggaagacgtt
     3781 acagatatcg tctttcgaca cgttgtaagt gaagcagcta gaccggatgt gtttttcact
     3841 gaatttacaa atactgaaag cttttgccac cctgaaggca tacatagtgt gcgcggacgc
     3901 ttaactttta gtgaagatga acagccgatg gtcgctcata tatggggaga taagccagaa
     3961 cagttccgtg aaacgagtat tcaattagct aaaatgggct ttaaaggcat agacttaaat
     4021 atgggatgtc ctgtagcaaa tgttgctaaa aagggtaagg gttccggctt aatcttaaga
     4081 cctgacgttg ctgccgaaat tattcaagcg actaaagcag gtgggcttcc ggtaagtgtt
     4141 aaaacacgcc ttggctacta tgaaatcgat gaatggaaag attggttgaa gcacgtcttc
     4201 gaacaagaca ttgccaattt atctattcat cttcgtacac gtaaagaaat gagtaaagta
     4261 gatgcacatt gggaattaat cgaagctatt aaaaatttac gtgacgaaat tgcaccaaat
     4321 acattgttaa caattaacgg tgatattccc gatagaaaaa caggacttga actggcagaa
     4381 aaatatggca ttgatggcgt catgattggt agaggcattt tccacaatcc attcgctttt
     4441 gaaaaagaac cacgcgaaca cacaagcaag gaactattag atctattgag attgcattta
     4501 tcattgttta acaaatatga aaaagatgaa atacgacaat tcaagagctt gcgtagattc
     4561 tttaaaatct atgtgcgtgg cataagaggc gctagcgaac ttcgacatca attgatgaac
     4621 acacaatcaa ttgcagaagc acgagcacta ctcgatgaat ttgaagccca aatggacgaa
     4681 gacgttaaaa ttgaattata gtatgagtaa taaagtttat ggatgatatt tcccaattta
     4741 acacggattg aacactttct acttaggtat tatcttggtt ttcctgatag gataactccc
     4801 gatgatgcct ttttacttat gtcacttatc cttttggaaa tggtgcaaaa ttaaaaatac
     4861 atcaggatat cgtttaactc agaaaaaaac tccaatcaaa aaaaatgttt acaacacatt
     4921 atgaaattaa aattgctctt agtgttcaac agacaaagtt attgaaatta ctattggagg
     4981 tacataccaa gctatcattt tgagtacacg tggtttgaat atctggcaag aaatggaaat
     5041 tggtaatgtc agtcataaag tcgctaaacg tgctcaaata ccattaatta tttttaaata
     5101 atttagagga tgaggtataa acctttaaaa aacagcagtg agatgatttt caattagaaa
     5161 atatcttact gctgtttcta ttttcataat cattcttatt gaatgacttc atcgtgatca
     5221 tttaaaacag taacaacttt ttcaatctta tactatttag agctaaaaat tcacgacata
     5281 taaacacaac actgacacgt catttaaata cttaatcttt tatagtaaaa gatttttcat
     5341 taaaaattta ttgttttctg tactttatta tattaatcct tttagcatgc cgtaccaata
     5401 cattttaggt aataaatctt tcttaaatat atacatactt ctacgttctt tggcctgatt
     5461 aaacggcatt gtttctttag tatttttatt ataatcaaac tctgcaagta ttaacctatt
     5521 atatccagta acaatagggc atgaagtata accatcataa tgatgcgtta acatttgatt
     5581 attcatcact tgcaataaat tattagcgac gataggtgct tgcttacgaa tagctgcgcc
     5641 tgtttttgaa gtaggtacat ttgaagcatc accaagtgca aatacattag agtagctttt
     5701 atgctgtaat gtggttgggt taacatctac ccaaccctca ctatctgaaa gtgtactttc
     5761 ttttactaca tctaagggac ccataggtgg tgttacatgt aacatatcat aacttattgt
     5821 ttttctatcg tatgctttga tatgttcgaa tgtagccact tttttgtcac cgtcgatttc
     5881 aacaaggtta taattataat tgactgttat atttctttct tcaacaatcc tttctaattc
     5941 tttattatat tttcctacgt caaataaagc atcttttggc gttgcatata tcacattagc
     6001 gttagaacgg attttatgtt tcctaaaata atcttcagct aaatacataa ttttcatagg
     6061 cgcacctcca cactttatag gagtgtttgg atgcgtaaaa atggcatttc cttgtttaaa
     6121 attagaaatt tggttccaag tttcgttaac atagtcaggt gaatagttag agcaaacacc
     6181 atttttacct atattttctt ttagtccttt aattgaagac caattaatct gtaatcctgg
     6241 agctactact aaaaaatcat aataaacgac tgtattatct cctaaaataa cgctattatt
     6301 ttcaggttaa aaacttgaaa cagcctgttt tatccagtta gcaccttcag gtataacact
     6361 ttccatatct ttacgagaac ttttcaaact agatacccct gcaccaacca acgtccataa
     6421 tggttgatag taatgatggt ctgctggatc tataattgct attttctctt ttaagttttg
     6481 attttttctt aatagtcttg atgctacggt aacacctgct gtaccgccac caataataac
     6541 aatttgataa tgcttattca ttttttccac atccttaaat tattttaatg attctggaaa
     6601 atcttgatag ccttctctaa tattcaccac attttcaaaa cctttgcttt ccaatatacc
     6661 cactgcaatt gaacttctaa cacctgactg acaatgtaca tatattttat cctctttatt
     6721 aaaaggaata ttttcattta ataatttacc atgcggaata ttaactgctt gatctaagtg
     6781 tccattattc cactcttcat cattacgtac gtctaataca tgttcttctt tacctgtcat
     6841 atcagcgcta tgaacggact gggttgaaat gcctgatttt ggcaaacgat agcctgctac
     6901 cttatcaaac ccaattaatt gtaaagtgtg tttcgctttc tcaacagtag atttatctcc
     6961 aattaaatct atatcttttt caaaatctaa gtaccaacca atttgattaa taaagttttt
     7021 gttgtatggt atattgattg ttccttttgt gtggccaccg tgaaaggcct ctttgctacg
     7081 aagatcaaat gctactctct tattatctaa actaggataa acatcatatg attgatataa
     7141 attcatacca aactgattaa cttgtttcat ttgtgcaaaa tgatgcggtg gtgctggttg
     7201 atttgatgtt aatgattcaa taaatttagt ctcatcaatt tcattaaatg cccagttatt
     7261 aattttctca taacctattg tagatatagg tatggcacct aatgctttac cacaagggct
     7321 tccagcacca tgacccggcc agatttgaac atagtctggt aaatttttaa tattttgaac
     7381 ggactcatac atttgtttcg cgctaatttc tgtagaaccc tttatttgaa cagatttttc
     7441 taataaatca ggtctaccta tatcaccaac aaaaataaag tcaccactaa ataatcccat
     7501 cggaacactt gaaccaccac ctaaatcagt gagtaaaaag ctaatacttt caggcgtgtg
     7561 tcctggagta tgcagaactt ctaatttaac attgcctact tgaatgatat ctccatgttt
     7621 aacaaattgt gtttttgatg gcatattttt ataccctaat gcatcttcac cttcgccaga
     7681 cacatatata tttgcattta agcgtttagc cacatcacga attcctgaag caaaatcagc
     7741 atgaatatgt gtttctgtag cttgtgtaat tgttaaacct tcagaatctg caacttctat
     7801 atatttcgat aaatcacgaa cagggtctat tattattgcc tctcctgtac gttgacaacc
     7861 cactaaatat gatgcttgag ataaatgatt atcgtaaaac tgtttaaaaa acatgataac
     7921 aactcctttt attaatagat ttaattagat aaataaatta tgattcgcat tttcagtagc
     7981 accaatataa gtgcctacac caccgtactc aacttcatct ctaagttctt ctttctgaat
     8041 acccatgaca tccatactca tcgtacaagc gattaatttg atattttgct caatagcttg
     8101 attgataagt gttggtaatg aatcaacatt tttctttttc attacgtagc gcatcatcat
     8161 atttcctaaa ccaaacatat tcattttgga aagaggcatt cgtatattct ttttgggcaa
     8221 cattaaatca aacatttttg caataccttg ctttttaaca ttaactgttt gcactttttt
     8281 taatgcatta agcccccaaa aagtaaagaa gatagttaca tcttttccag cagctctagc
     8341 accatttgca ataatcaacg ctgctacagc tttgtctaat tctccactaa ataatacaat
     8401 tgtagtacct ttagcagaat aatttatatc taaatctttt gctttttctt tttgaataat
     8461 cgcattaatt ccattgttat tttcatcaag cttaactaaa gtatgccttg tttgtttcac
     8521 ccaactttta atgtcactag ggaatccagg gtctgtgact ttgacttcaa tttggtcacc
     8581 tacttcaata ttcttcattt cttgactaat ttttacaatt ggccctggac attgaagacc
     8641 acgatagtta aattgtttac gatctgcttt aatttctata cttttatatt cttcttgtgt
     8701 atcataacta tcgttttctt cttcataagc tttataacca ccatcaagat ttataacgtt
     8761 ataaccttgt ttagctaaat atttactagc attagcactt ctgttaccac ttctacagat
     8821 tacataatag gttttatttt tttctttatt gaatgactca atctcatcaa caggatgaag
     8881 tatggaacca ttgatatgtc ctaatgcata ctcctcttct gttctaacgt caattaattg
     8941 cccttgcttg gctagttttt ccaattctgc tttactaaat tcatcgataa acttttctcc
     9001 gtattgtttc ataataacct ccctaaaaat acctgtaggg gtattttaaa cagttggatg
     9061 gaacttgtca aatacctcta ggggtatttg acacatttgt gtttatctat taatatattt
     9121 ttattcaatt aaaagtggag gtatgactat gaattatgat aaaaaaatga ttaatcgtat
     9181 taatagaata caagggcaac taaatggaat tattaaaatg atggaggaag gaaaagactg
     9241 taaagatgtc attacacaaa taagtgcatc aaagagttca ctccaacgct tgatgggtat
     9301 cattattagt gagaatttaa tagaatgtgt aaaagcagct gcggatgatg aagaaagctc
     9361 ccaagagtta attaatgaag ctgtaaactt attggtgaaa agtaagtaat ggatattgtt
     9421 aatatagtta tcatgttact cattggggta ttcggtgggt tcatatctgg tctagtaggc
     9481 gtaggtggtg ctatcattat ttatccagct attttattat taccaccatt attcggagcc
     9541 cctgcatata gcgcatatat cgcatcaggt cttacttcta gccaagtctt tttcagtaca
     9601 ttaagtggct cattaaaagc tcgaaaaaaa acagagttct cgccacaatt agttctttat
     9661 atgggtggcg gcatgattat agggagtatg ttaggtgcat ttctagcaaa tttatttgat
     9721 gctacatttg taaatacggt atatatcatt attgctttac ttgcattaac attaatgttt
     9781 attaaagtga aacctagttc cgaaaagtct tcatttaata aatatttatt agttatcatt
     9841 ggccttttca ttggtattat ttcaggtata gtaggtgcag gtggtgcctt cattattatt
     9901 cctatattat tggttttatt caaattacca atgaatactg tagtcgctaa tagtatagta
     9961 attgctttta tatcttcaat tggtgctttt gtcatcaaac ttattcaagg ttatatccct
    10021 ttatatgacg ccttattcct tataataggg agtattatat ttgctccaat agggttaaaa
    10081 ttaagtaaaa aagtccctaa cataatacaa aaatggatta tcagtatttt aatcgttttt
    10141 gctatagttc aactcatttt ataaaactaa tccttttaac tcagacagca atttcccgaa
    10201 aaataatacc tcttatttaa acaaatatgt atgcagttcg gatttatata aataaaaatt
    10261 tttgatgaaa tatccatata tgcttataaa tataaattta tcaactataa ctaaaaatga
    10321 taaaagattt aacgccactg atgaccgaat taatattatt taagttttta tcaaacgcat
    10381 gagtaaagta gatgcacatt gggaattaat cgaagctatt aaaaatttac gtgacgaaat
    10441 tgcaccaaac acattgttaa caattaacga tgatattccc gatagaaaaa caggacttga
    10501 acttgcagaa aaatatggta ttgatggcat catgattggt agaggcattt tccacaatcc
    10561 attcactttt gaaaaagaac cacgcgaaca cacaagcaaa gaactattag atttgttgag
    10621 attgcatcta tcattgttta acaaatatga gaaagatgaa atacgacaat tcaagagttt
    10681 gcgtagattc tttaaaatct atgtgcgtgg cataagaggc gctagcgaac ttcgccatca
    10741 attgatgaac acacaatcaa ttgcagaagt acgagcacta ctagatgaat ttgaagccca
    10801 aatggacgaa gacgttaaaa ttgaattata gtatgagtaa taagggaaag gaggaattta
    10861 agtttcctcc tttgtttgtg tgaaagtctt ttaggtatga actagggtga tttaaaataa
    10921 aagtttaatt tctatcttca gttggcgttt ccatataaat aattggatta attctttgat
    10981 cttttaaata gagagaccac tctactaatt tatttttaag taatactgaa cctaaattag
    11041 ttaattgaaa gtttttattt attacgttac tagtatgaac catatctttt atgggcatat
    11101 tatcaataga tgatatcggt aaaatccata ctatactaaa atttgtactc cgttgtatat
    11161 cattaaatat attttccgag ttatcaggaa tagcttctat taattcttca aaattatatg
    11221 attctggata ttttaaggcg ttaattgcta ttatattctg ttctagacta taaagagata
    11281 aagatgtatt aattcctctt ttaatcgtga aaagctcttt gtttttaata ttgtataagt
    11341 ctgccaactc tattccattt ccttttacta taaaattctt atttattggt tttatgaatt
    11401 ttttatgtaa cattaaatat tcttctggca atcttcctac tatttctttt atatatcctt
    11461 cttctccttc tttaaatact aaacctgtgg gagttagtgt gttataccta atttctatct
    11521 cattcaaatg atcattcaaa aggtcaaaaa attctctatt aaaataaccc catttcccat
    11581 tttgtaataa atatttcttt ccatcttcca tttcaacttc tgcatgtagt atcgttttta
    11641 gtagcata
//
LOCUS       DANJFZ010000084        11481 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.084, whole genome shotgun sequence.
ACCESSION   DANJFZ010000084 DANJFZ010000000
VERSION     DANJFZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.084"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..766
                     /locus_tag="PWN45_001943"
     CDS             <1..766
                     /locus_tag="PWN45_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0623705.1"
                     /translation="YADKFIDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDT
                     ERKLNSKEFYNIKRKDKQTIIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEE
                     KQRKVNQNILINIPIKSENNKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPK
                     AKAFIEDFFNKYASSKSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEIL
                     MKDKDSPLENLEHYTLDITKKDGKYYVKNMTNSIGG"
     gene            771..1031
                     /locus_tag="PWN45_001944"
     CDS             771..1031
                     /locus_tag="PWN45_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623706.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            1043..1426
                     /locus_tag="PWN45_001945"
     CDS             1043..1426
                     /locus_tag="PWN45_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0623707.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1461..3956
                     /locus_tag="PWN45_001946"
     CDS             1461..3956
                     /locus_tag="PWN45_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0623708.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            4010..5368
                     /locus_tag="PWN45_001947"
     CDS             4010..5368
                     /locus_tag="PWN45_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL0623709.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            5373..7220
                     /locus_tag="PWN45_001948"
     CDS             5373..7220
                     /locus_tag="PWN45_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623710.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            7210..8256
                     /locus_tag="PWN45_001949"
     CDS             7210..8256
                     /locus_tag="PWN45_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0623711.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            8263..8853
                     /locus_tag="PWN45_001950"
     CDS             8263..8853
                     /locus_tag="PWN45_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623712.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            8909..9265
                     /locus_tag="PWN45_001951"
     CDS             8909..9265
                     /locus_tag="PWN45_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HDL0623713.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            9374..9877
                     /locus_tag="PWN45_001952"
     CDS             9374..9877
                     /locus_tag="PWN45_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0623714.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            9858..10394
                     /locus_tag="PWN45_001953"
     CDS             9858..10394
                     /locus_tag="PWN45_001953"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0623715.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            10614..11162
                     /locus_tag="PWN45_001954"
                     /pseudo
     CDS             10614..11162
                     /locus_tag="PWN45_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         4197 a         1269 c         2145 g         3870 t
ORIGIN      
        1 atatgctgat aagtttattg atacgtatat gaatatctcg aaagattcaa aagaattgga
       61 atctagagaa aaagaattgt tgaaatattt tccatcagat tataaaaaac ctgatgaaaa
      121 aatttcagat acagaacgaa aattgaatag taaagaattt tataatatta aacgtaaaga
      181 taaacaaaca attatacagt atattgttaa ttatgatgtg aatattactg aaaagaaaga
      241 agttaaagtt aagaagaaaa agaaaagtga aaaagataaa gatgagtatg aaacaaaaac
      301 agaagaaaag caacgtaaag taaatcaaaa cattttaata aatataccta ttaagagtga
      361 aaataataag tatgttgttg ttgaatatcc ttattttacg ccaatccctg atagtcaatt
      421 gaatcaagct aaaatggttg aggataattt gaaagacaat aaacgggaag acaatccgaa
      481 agcaaaagct tttattgaag atttctttaa taaatatgct tctagtaagt ctgatgatat
      541 ggcctattta atggataacc ctgagggctt agaaggaact agagaagttt ctcagataag
      601 agaaattagg ttatatccaa aaggcgatga ttatgttgct aaagttgaaa ttttgatgaa
      661 agataaagat tctcctttgg agaatttgga gcattacaca ttagatataa caaaaaaaga
      721 tggtaaatat tacgttaaaa atatgactaa ttctattgga ggttaatgtt atgagtggtt
      781 tatttaattt ttttgtttta ggtgctgacc gtccatcact tggtggagtt ggtgactgga
      841 taagtaatga agtaggtacg ggtattggtt tagtagtatt ggttgttggt attgtaaaat
      901 gggctagtgg taaatatggg catatggtta tattatttgt tgttggtggc tttttattct
      961 tagtttctaa aggtcctgaa caggtattta atgcgattgc aggtgtttgg aaaatgattt
     1021 ttggcggttg aggtgattcc atttggataa aaaagcttat aatttaaagc gaacttttga
     1081 acaaccgata gttatgtatg aatttacaga taagtttcgt attaataagg gttttcgttt
     1141 agatttttgg gctacatttt tgattgtgtg gtttatatta tttttgttat tttggtattt
     1201 attacagcct gtaattatgt cgattggtgg acttatgttt atttacttta cggttgcacc
     1261 ttattatata acgaaatata ttgttaagtt aaaacaagat ggaaagaaat tatttttctt
     1321 cttatgggat tttgtaatat ttgtttttaa tgtgcagtta agaaaagtta aattaagtta
     1381 tgatgaagaa gtagaatatc atgataaaaa aataacattt aaatagcccc tattggggtt
     1441 atttttattg gagggatgac atgaatttaa aagcaccgta tgcaggttta agagataatt
     1501 tgttattaac gaaaactggc gaagtttggg cgtattatcg tgttcgttct gagtctatat
     1561 caacagcaaa ttatgatgca aaagaagaat caaaaaagcg tatgcgttat tttttagagt
     1621 cgattaaagg ttatcaagat tttcattttg aaatgtatga ccacgattta gatttgcgaa
     1681 ataaatttaa agaaattagt aaagattttg atgatgaagc ttttaatgta gctgaatatt
     1741 attcaaaaga aactattgac gtattagaac aagaattaca aatgattacg cataattctt
     1801 ttgttatggg tgttaaaata cgtgaattag ctgacgatgc agttacactt aaagagattt
     1861 taaaaagtac attatcagat acggctgagc gtattgtttc gaattttggt tttgatgtga
     1921 atttagacga taaaatttta gataagtata aaacatttga acgagatttg gctgatagtt
     1981 ttctaggtat tggaggcgag cgtttaactg agaatgaatt ggcttatatt attagaaagc
     2041 cttttatttc aaatgcaaca catgacgtac aggaagaatc ttcaagaaga gctattacag
     2101 atatttataa tgcagtaatt gaccctactc gcttgagtgt tttagaagtt gaaaatgata
     2161 acgagaaatt ttatacaaca acagttgttg ttgatgattt tccgttagat atggaatata
     2221 cacatttatt tgagaaagct caaaatatgc cttttccggt tgaatgccat gtaaaagcaa
     2281 aaatcattaa taatgagaaa gttaagaaaa aatttgaccg tgcgaaaata aatcataaac
     2341 aacaagcaaa agaaaagcaa gatactggcg acagtgcttc ggaagatgtt caagataatt
     2401 tgtttgtatt gaatcaaatg gaacgagaag tgactggctc aggtgtattt attgagtggg
     2461 tatttatgtt tgtaattaaa agtgatgatt atagagattg taaacgtaaa gcaaagcgtt
     2521 tgatgaaacg attaaaggaa actcaaatat atgcagttaa tccattagct gaccagttac
     2581 aattgtttta tcaatgttta catggtaatg atttatttat taataagtat tggttacaaa
     2641 gaacaacggc aacaggtata gcagagaatt tatttggtgt gtctcaaagt ttgggaacaa
     2701 aaacgggttt ttatataggg cgtattgata aatttatacg ttctgtttca cgtgaggaag
     2761 ctgttgcaag ttcaagggat ataattttgt ttagtttatt gttggccgca aaaggtatta
     2821 agggggctgt cagtgatagt ccgcatgttt taattactgg tcaaactggg aaaggtaaat
     2881 catttttagc aaaattatta tggatttata catcattttt taaaggtcaa atgttgtact
     2941 gggatccaaa atctgagttt gcagaatggt ttgacagagt aacagaaagt aaggaaatgc
     3001 aagaaaagta tcccctattt attaatcatt taaaaacatt taaatatgta acattgaatt
     3061 atgaagattc aaccaattat ggttgtctag accctattgt atttttagat ggtattgaag
     3121 ctaatgagat gttgaaatca gtgtttgatg aaattaaatc ttttgaaaat tatcatcata
     3181 ttgaaacagc aatttatcaa gctatttctg atacggttga agaacgtgaa aatggtaaaa
     3241 aagttggttc attaaatgta attgaaaaat tacagaataa tgaagatgag catgtaaaaa
     3301 atgcaggcga tttattattt gaaaaaacgc aaaacaatat tttaaaactt gtttttagtg
     3361 atggtacgaa tccagcttta aatattcaag aaaaagcaac tattttacag gttaaaggtt
     3421 tagatatgcc taaagcagat gatgatacat caagttattc aacaagtgag aaaaatggga
     3481 ttacattaat gttattaatt ggtaagtttt tagagaaatt cggttctcgt agagatgttc
     3541 aaacgactat atttattgat gagggttggg cttttagtgc ttcaagacaa ggtaaaaaag
     3601 ttggtaagcg tataaaaaga acgggacgtt ctgagaataa ttcacttgta tttattacac
     3661 aatcggtaaa agataaagca gatgatgatg gtggtaactt tggttgtcat tttgcatttg
     3721 atgaaaaaga tgaacgtgag gatattttaa aatctttagg cttagaatat tcaaaagaat
     3781 ctcctgaaaa catggagatg ttgaaagatt tgaagaaagg tcaatgtata ttcagtgatt
     3841 tttatggacg tgttggaaaa atggttgtac attgtccatt tgaggaaatg actgaagcat
     3901 ttagaacaca agaagattct gcaagctcta aagctgaaga aaaatttgcg atgtaatttt
     3961 tagcacatac aaaattgtat gtgcttttta ttttaaagga gtggatttga tggcaatgaa
     4021 tgaaattgct ttatttaaag gcgtacgtat acagccttat cagaagtata ttgattttat
     4081 gtttgcaagt gtattagcat ttattttttt ggtatatcgt atatatattt tgtttattga
     4141 ttataaatca gttaataaaa aattggatat attgacgttg tttgcatatt ttaaaccgca
     4201 tttattgtat ttattaattg gtacagcaat tatatttgtg atatgtaagc ttattgctca
     4261 gtttttaata cgttttaaaa caaaagattt ggctgaaatg attgaaacac aaggttttca
     4321 taatcgtaca gtaatcaaga aaacaactga gtatatccct attgattata aacaaactga
     4381 atatgattat tatccaacaa tgttttataa gcgtggtaaa aaatcttttg ttattcatat
     4441 taagaaagac ggttcgaggt ttcaagataa ttatttagag ttagagacga ttattgaacc
     4501 aatgtttaat tctgaattag tagaaaaaaa gcatattggt cgttatttga gatatgaatt
     4561 tatgccactt aaatataaaa agcgtattgt tatgaatggc gaacaagaat caaatacaat
     4621 tttttatgat acaaaaattg ctattacgca tcaaattact tgggattttg tgaaagcacc
     4681 tcatggattg gttacaggta taaccggagg tggtaaaaca tattttttat tttatgtgat
     4741 tcgtgagtta ttccgtagac attcagaagt acgtttatta gacccaaaag tttcagattt
     4801 gagctttatg aagcgcgtta ttggtgatga taaagttgca gatactaaag gtcaaatatt
     4861 aaaacaattg agagaagcta ataatgaaat ggaagagcgt tttagattaa tgaatgatag
     4921 ttcggattat aaaatcggga atgatttccg taattttgat atgcgcccct atttcattat
     4981 ttttgatgag gtaacagctt ttacttcaac tttagataaa aaagaattac aagaaatgaa
     5041 tgattattta attaatatta ttatgaaagg tcgtcaagct ggtgtattta tgtttttaac
     5101 agcacaaaga ccagatgcag atgtgattaa aggtaatgtg cgtgaccaat tagggttacg
     5161 tgtgtcttta ggtaatttgt caaatgatgg ttatcgtatg acctttggtc aaactgataa
     5221 agaatttcaa acgattcatg atagtgatat cggccgaggt tatatttcta tacttggaca
     5281 atataatgaa ccaattttat ttgatgcacc tttaatggaa caatatgatt ttgtggaaga
     5341 tgtgaaacaa atattaaata aggagtgatt tgatgaagca taaaagtgtg tatcttttat
     5401 gctttttaat tttgcctatt ctgcttttaa catctgtgag tgcttatgct gtttctaatc
     5461 cagttggaga gccctcacat gcagttaagc caaaaattga aaagtatgat ttatcacatt
     5521 atcgttcaat atatagtgaa aaaggcgact ggaatccgtt tggtcaagaa gaaatttcac
     5581 gtcaaattaa taatgtgtca gattttttct tttctatgac taagatttta gcaggtgtta
     5641 cagattatgc gattgaaaat ttatttcaat tagatgttat taatgatttt gcggataaaa
     5701 tcggcgattt tgttggcgat atatatcaaa agttattgag taatttagca ttaacattat
     5761 ttattattgt ttgttttaac gcatttatta tttttagtgt acaaggtaat gcaagagaag
     5821 cattaaaacg agcgttttta attatgtgtt taattggttt tggtgttggt atacttgcga
     5881 atgcaggtaa tattatacgt ggtacaaaca atattggtaa ggacttaaat aatataatta
     5941 tgaatagtac atcttctatt gatggtaata ttgattatat tcatgagaat tcaggtatga
     6001 atcatatacg taatcaatta tttgatatga cgatatatag aacatattta gtaatgaatt
     6061 atggaactgt cgacgaaaaa gagataaaag caaaaggtaa agaccgtatt gataacattt
     6121 taaaacttga ttatgataaa aaatcagaaa aagaattaga tgatattgtt aaagatgagg
     6181 ttgaaaagaa aaataataaa tatatgacgc aaggttatgt atttcaaaag ttggctattt
     6241 cagtgattgg ttttattatt acacttttta tgtcgatagt atttttatca ataagttttg
     6301 cgaagttgat tttttctaca tttgctttat ttttattctt atttttagta ttcagttgga
     6361 tagtgagttt tataccagga tttgaattat ctgttttctc tgcgtttgca aaaacgttag
     6421 gctatattat tttaagtgct tgtatgacat tcttatttgt gatagttggt ttgtgtatca
     6481 agcttgcaaa tagttttatt gaaccggata gtcaaaatgc ttatttttta aattcaatat
     6541 ttataattgt aatattgttt gtaatgtata aaaaacgggc tcaaattatc aattttgtaa
     6601 gtcgtggtaa tattagtttt tctcctagtg caattggtgc tggtgtgatg aatcgaacac
     6661 aagaacgttt taataagatt cgtcatgaac aagcgcaaaa taagaaagct aaacgagaaa
     6721 atcaaagaga tgaacccgcc ccaccacttc aaaatgataa tgatttaaga agaagacaac
     6781 aggataaacc aatgccttta tttataaata aagataatca aaaaaatggt aataagcgca
     6841 gagaacaaca agagtcaatg aatggtaacg atgtaaaatc tgcttctgtt gaatcaaatg
     6901 caaataatta ttctaaacaa ccacaaaaag catcacagca agaacatcaa gtacgagaaa
     6961 caagacagcg aaaagatata caaaggtcgc cacaagtggt taatcagcca ttaaataatg
     7021 agaatcattc aataaatcgt aaagaacaga agtcagttca aactgcttat gatactgatg
     7081 ttcaaaaacg acaaatacaa aatgcgacac agaatcaaca aagtagacaa tctggaaatc
     7141 gtaatcaacc tattacgcgt aattctcagt caaaagaccg tttaaaagag caaaaggata
     7201 tcaataagca tggtaaataa aaaagatatt gctaaaaaag taataggtaa aacgccaata
     7261 ggcgttaaat tgaaattagc gaaagtcatc atcattttat gtgtggtggc tttttttata
     7321 tttccagtaa ttataatgtt tttgttagcg cctgatttaa ataaaggtaa agatgatgct
     7381 ggttgtactg ttagtggcgg taatgtttcg gcaaatggta ttgataagtt taacgaaaat
     7441 gctaaaggtg gaaaattaga gggtaaagga aaagaaatac agaaaattgc agaaaaaaat
     7501 aaagtacctg tgaatatttt tatggccatt attgcgagtg aatcacaatg gggtaaagga
     7561 gaaaatgcga caagacaaaa taatcctttg tctgttatgg gttcaaaatc gattcatgat
     7621 tcaacatatc caactattga agatggtctt aatgcgggag ctaaaaattt gtatgatgtt
     7681 tatatttcta aaggtttaga tactcctaaa aaaataggtc ccaaatatgc gcctgttggt
     7741 gcgtcaaatg accctaataa tatgaatgcc cgttggatac caacagttga aaaaattatg
     7801 aaagatttag gcggttcaga agctaaaacg tcatgtagta atggtaaagg aaagtctata
     7861 aagtttaatg gtaaattgcc acattggtca aatgatgacc ctggaaaagg taatttatac
     7921 actgctggtc aatgtacttg gtatgcatac ggtatgcgtc aaaaaatggg taaacctgta
     7981 tcgacttatt ggcatgatgc acataaatgg aatgatagag ctaaagctga gggttataaa
     8041 gttgataaaa atccggaacc tggtgcatta tttattgctg aacaaggtgc aggcggtcat
     8101 gatgggcatt atggtcatgt ggctgttgtt attggtgtaa gtgatggtgg taaaacattt
     8161 agaataacag aaatgaattg ggaaggtgct tttaaagtta atgaacgtac attgaaaatg
     8221 actgacggat atagttttat tcatgataag gagtgaaaat aaatgaagca aaaaattatt
     8281 atgtttactc ttgtaacagt aatattattt tgtgctgttt taattggtta tcagattcct
     8341 aaacaacaag ttaaaatgaa acaaaatcaa attgaagatt tgcaggaaga acaacgtata
     8401 ttaagagata aaaatggaga gttaaataaa ttagtaaaga gacaatcaaa aacagttatt
     8461 tctgatgaag aaaaacaaat acgtgaagtt tcttcaaatt ttgttaaaca gatgtttgaa
     8521 atgaaaaaag attcttcttt taaatcaaaa gcaccacaaa taaagccttt agttacaaag
     8581 gattattacg atacattatt taaggattct aaagataaat atgatttata tgatgatata
     8641 acggttaatg atattcatgt ttattttgat acttatgacc ctaaaaaaga tagttataaa
     8701 gtttttgttc agttcgacga acgtattgaa acagatggag atgataaaat tgaacatcgt
     8761 caaacatctg cacaattgga tttagttcgt acagctgaag gttggcgtat tgataattta
     8821 aaacgtttca atttaaaacc tctaggacgt tagtttgttt caagacgata gcaatgatat
     8881 aatttacgta aaaaggagtg aatttatcat gaggcgatgg tttgtattaa ttttaggatt
     8941 agttatatta ttatctgcat gtggtcagaa atatgataaa gaaattgatg aagttacaaa
     9001 gttagaaaaa gaaagtattc aagatgtgaa aaatacaaag aagtataaaa atgttgagcg
     9061 gtcaaaaagt tattataaga tttataatga tggtgaggtt attataatga cttatatgcc
     9121 ttttaaagat agtaatacaa aagtttcaag agtatataaa ataaatcaga ctagcgataa
     9181 atatgaagaa gattctaata ttgatgctga aaagtttgag aaagataata aaccagttta
     9241 tgaagaaaat aatatgaaaa aataaagaat caactcccta tacgctttag cgatggtatc
     9301 gggagttgat gaacatatat cctcttataa cttattttat aaataacctt tttaaaagta
     9361 aaggaggata tacatgaaaa aaagaaaaaa acgtaaagaa ttagactata aaacacgtct
     9421 agctatcgca aagtatttaa atgatggttt aaacatcatt cagatttcta agattttagg
     9481 tcgtgatgat gcagttcttt atagagaagt aaataagcgt tctgttgatg gtgtttataa
     9541 tcctttatta gctgaggaag atgctataaa atcgcgtagt atttcagatg aacaagttgc
     9601 taaacgtaga cgtttgaatg atgaaacaaa agcatatatt gaagaaaaac tttcattaaa
     9661 atggtctcct agacagatag caagtcaaat tgaaaaagat acagggtatt atataagtta
     9721 tcctactata tatagatata ttcgcaatgg attggtaaaa gttgatgtta agcgtgatat
     9781 gagacaagca ggcaagaaat ataataagtc ttctgaaaag cgtggcaaat tagacgtagg
     9841 gtctcgtgtt ataaagtatc ggctcaaaaa gtattaataa tcgtgaagaa tttggacatt
     9901 atgaaattga tactgtttgg agtgttagac catcaacata ttgcttgtta actattattg
     9961 aaagaaagac aagatattta tatgctagta gattaaatac acgtaaatcc aatgtagttt
    10021 gtaatgagat tataaatatt atgaaaccat tattacccaa aagtataact atggatagag
    10081 gtaaggagtt tgctctattt gaagaaatag aagatgaatt gaattgtagt atttatttta
    10141 gtgatttggg ttgtccatat caacgtggtt ctattgaaaa tgcaaatgga cttttaagac
    10201 agtattatcc taaaggtacg gattttgcaa atataacaca aaagtcatta gatgaagctg
    10261 ttaaacaaat taatgcacgg cctcgaatga tatttgacta taaatcgagt gaggaaatgc
    10321 taaagtacca tgtaagcaca caaaattgtg aacctatatt aaatgactgt gtgaggcacg
    10381 aacccgtcaa ttaatatagg ttaatctaag cgaatggttt acaatgagcg cggaaggaca
    10441 ggacgaataa attttgtatg taaaatgcat aatctaaaaa gaagcatact atttaaatct
    10501 gtggtttttg acagatatta ctagtaaaat gcttcttttt cttcttctgt actcttttag
    10561 taatatttat taaaaaaatt taagaaattc tcatttttct attgacacac tgccatttaa
    10621 ttaaagcaat tggagtattg ttactcattg tcggcatcgt cataatgaat caatttaaca
    10681 agaataattt attactaact gatcaaaagt atttactgtt ttggcttcta ttaggattta
    10741 tttttggttt ctttccacct attcaaacga caattaatag tgctttagct agtcatactc
    10801 attcaccagc ctttgcatca ttagtatcat ttacaattgg gtcaatagcg ctattgattt
    10861 taaccgctat ttttaatcgt tctttaaaac taaaaacaag tcatttaaaa ttcggtaaat
    10921 taaagcctat ctattttact ggcggtatac ttggcatggc ttttgtaaca gctaacatta
    10981 tcttaatgcc tcatatgggt gcagcattaa caacacttat tgggatgttt ggccagattc
    11041 taatgggcat attgatagat cactttggat tatttggttc acctaaaata gcaatgacat
    11101 ccagaaaaac tattggtcta ttatgtattt tgacaggcat tatactttta agattatttt
    11161 aaattaactt ttagcttatc attttaactt gtaattattt ttaaaagtga taagctattt
    11221 ttttgtggtc taaaaatctt tagaaaagcg ttgatttggg tagtatgctt tgacacaaca
    11281 aattttaatt tagcaaattc gatagtcaac tcattcttaa gacctaaatt aatgttattt
    11341 tttaataatt tacaccaaat taatagcaaa aattatgtta ttcgtgctaa tatttcatag
    11401 ttggttattc aattaattaa aaataagtca aaatgcacaa ctttttataa ttcattgagt
    11461 cgagtttgaa aaataaaagt g
//
LOCUS       DANJFZ010000085        11408 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.085, whole genome shotgun sequence.
ACCESSION   DANJFZ010000085 DANJFZ010000000
VERSION     DANJFZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.085"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(57..896)
                     /locus_tag="PWN45_001955"
     CDS             complement(57..896)
                     /locus_tag="PWN45_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0623716.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1044..1151)
                     /locus_tag="PWN45_001956"
     CDS             complement(1044..1151)
                     /locus_tag="PWN45_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623717.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(1305..2415)
                     /gene="prfB"
                     /locus_tag="PWN45_001957"
     CDS             complement(join(1305..2342,2344..2415))
                     /gene="prfB"
                     /locus_tag="PWN45_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL0623718.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(2584..2688)
                     /locus_tag="PWN45_001958"
     CDS             complement(2584..2688)
                     /locus_tag="PWN45_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623719.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(2730..5261)
                     /gene="secA"
                     /locus_tag="PWN45_001959"
     CDS             complement(2730..5261)
                     /gene="secA"
                     /locus_tag="PWN45_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL0623720.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(5675..6247)
                     /gene="raiA"
                     /locus_tag="PWN45_001960"
     CDS             complement(5675..6247)
                     /gene="raiA"
                     /locus_tag="PWN45_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL0623721.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(6308..6982)
                     /locus_tag="PWN45_001961"
                     /pseudo
     CDS             complement(6308..6982)
                     /locus_tag="PWN45_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            complement(6975..8057)
                     /locus_tag="PWN45_001962"
     CDS             complement(6975..8057)
                     /locus_tag="PWN45_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0623722.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(8421..9287)
                     /gene="fakB1"
                     /locus_tag="PWN45_001963"
     CDS             complement(8421..9287)
                     /gene="fakB1"
                     /locus_tag="PWN45_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDL0623723.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            9431..10072
                     /locus_tag="PWN45_001964"
     CDS             9431..10072
                     /locus_tag="PWN45_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL0623724.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(10242..11297)
                     /locus_tag="PWN45_001965"
     CDS             complement(10242..11297)
                     /locus_tag="PWN45_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL0623725.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
BASE COUNT         3585 a         2081 c         1619 g         4123 t
ORIGIN      
        1 cctctcaacc tgttacttaa acaggctaag aggatagtct tgtcttgata tattacttag
       61 tggatgtaat tatattttcc tggatttaaa attgttcttg aagatttaac attaaatcca
      121 gcatagttca tttcagaaac agtaattgtt ccattagggt ttacagattc aacaacacca
      181 acatgtccat atggaccagc agctgtttgg aaaatagcgc caacttctgg tgttttatct
      241 actttaaatc ctgcaacttt tgctgcgtaa ttccagttat ttgcattgcc ccataaactt
      301 cctatacttc tacctaattg tgcacgacga tcgaaagcat aatatgtgca gtttccataa
      361 gcatataagt ttcctctgtt agcaactgat ttattgtagt tatgtgcaac aggtacagtt
      421 ggtactgatt tttgtacttg agcaggtttg tatgctacat taactgtctt agttactgct
      481 tgcttaggtg cttgcttaac tactactttt ttagatgctt gttgtacagg ttgttttact
      541 acctttttag cttggcttgc ttttcttact ggtgatttaa ccgctttagt ttgtttcact
      601 ttattttgag gcacaagtga aatcacgtca ccaggaaaaa ttaaaggtgt tacaccagga
      661 ttgtatgaat ataattgatt caacgttaag tgatgctcta aagcaatctt atataatgaa
      721 tcgccagcaa ctactgtata agttgtcggt gattgcgttt gtgcttgaac atttgataca
      781 taattatgtt gaacaggtgt ttttacttgt gtgccatgtt gttgtgcatg tgctgcatta
      841 tttaaagcta aaaaagctaa cactgacgaa accgtcactg taagagattt tttcatcttg
      901 ctgtcattcc tttgctgtta gtattttaag tatgcaaata ctatagcaca atacattttg
      961 tcaaaagcta ttgttataac gatgtaatca aatggttaac aatataaaaa gaatacaacc
     1021 ttttatcata gtgtaaaatg tattcatacc atgtaattga gaacgttttc aataattaat
     1081 tcaatacctt gaaaatcgcc ataggtaata ttactaaatg cacactgcat atgttgtttt
     1141 aacaaacaca acttttaaaa aatatattct aactctatct accgaattgt acttaaatat
     1201 tcataaacaa atcatattcc aaaatctaat ttacaattta tttagctacc tttaaaaaaa
     1261 ccaaaaaccg acgccctttt agagcctcgg ttttaaaata tatattaatc gtgcgacatt
     1321 gtctgtctta aatatgattc gataaatggt ccaatgtctc catccatcac tgcatcaacc
     1381 ttacctgttt cttcgttcgt acgatgatct ttcaccattg agtatggatg gaaaacatat
     1441 gatctaattt ggcttcccca gccgatttct ttttgttcgc cacgaatttc agccatttca
     1501 cgtgcctgct cttccaattt taattgatat aatttagact ttaacatttt catagctgct
     1561 tcacggtttt taatttgaga acgttcattt tggttattaa caactatacc tgaggggtgg
     1621 tgggtaattc gtattgccga ttcagttttg ttaatatgct gaccacctgc accagaagct
     1681 ctgaatgtat caactgtaat atcatccgga ttgatttcaa tctctatttc atcattatta
     1741 aaatctggaa taacgtcgca tgatgcaaat gatgtatgac gacgtcctga tgaatcaaat
     1801 ggagaaattc gtactagtcg gtgtacacct ttttcagctt ttaaataacc ataagcatta
     1861 tgccctttga tgagcaatgt tacactttta atccccgctt catccccagg tagataatca
     1921 acagtttcaa ctttaaagcc tttcttctca caataacgtt gatacattct aaatagcata
     1981 ttagcccaat cttgagactc cgtgccacct gcaccaggat gtaactctag aattgcgtta
     2041 ttggcatcgt gaggcccatc taataataat tgcaattcgt attcatccac tttagcctta
     2101 aaattaatga cctcttgctc taagtcttct ttcatttctt catcaaattc ttcttgtaat
     2161 aaatcccaag tagcatccat gtcatctact tctgcttgta gtgttttata accattaact
     2221 attgctttta acgcattatt tttatctata atatcttgcg ctttcgtttg gttatcccaa
     2281 aaattaggtt ctgccatcat ttcttcatat tcttgaatat tagtttcttt gttctctaag
     2341 tcaaagagac cccctaattt gtgttaaatc ttgattatac ttatctatat ttcgtttgat
     2401 ttctgataat tccatagcat tcgctcctat ttatatttca attcaagtca ttgatttgca
     2461 tcttttataa tgctaaattt taacataatt ttgttaaata acaatgttaa gaaatataag
     2521 cacactgaca attagtttat gcatttattg ttaaaaatca gtacatttat catcgacata
     2581 tgcctaaacc gattttttaa aactaagtac ataacaacgt ttaacaactt cttcacattt
     2641 tttaaagtat ttaacgcttg taaaataaaa agactcctcc cataacacaa actataggtg
     2701 tttaattgga aggagttatt ttatatcatt tattttccat ggcaattttt gaatttttta
     2761 ccactaccac atggacaatc atcgttacga ccaacttgat cgcctttaac gattggtttc
     2821 ggtttcactt tttctttacc atcttcagct gaaacgtgct tcgcttcacc aaactctgtt
     2881 gttttttcac gttcaatatt atcttcaact tgtactacag attttaaaat gaatttacaa
     2941 gtatcttctt caatattttg catcatgata tcaaataatt catgaccttc attttgatag
     3001 tcacgtaatg gattttgttg tgcataagaa cgtaagtgaa taccttgacg taattgatcc
     3061 attgtgtcga tatgatcagt ccaatggcta tcaatagaac gaagtaaaat catacgctca
     3121 aactcattca tttgttcttc taagatatct ttttgacttt gatatgctgc ttcaatctta
     3181 gcccaaacga cttcgaaaat atcttcagca tctttacctt tgatatcatc ctctgtaatg
     3241 tcaccttctt gtaagaagat gtcattaatg tagtcgatga atggttgata ttcaggctcg
     3301 tcatctgctg tattaatata gtaattgata ctacgttgta acgttgaacg tagcattgca
     3361 tctacaactt gagagctgtc ttcttcatca ataatactat ttctttcgtt atagataatt
     3421 tcacgttgtt tacgtaatac ttcatcgtat tctaagatac gtttacgcgc gtcgaagtta
     3481 ttaccttcta cacgtttttg tgctgattct acagctcttg ataccatttt tgattcaatt
     3541 ggtgtagagt catctaaacc tagtcggctc atcattttct gtaaacgttc agaaccaaaa
     3601 cgaatcatta attcatcttg taatgataaa tagaagcgac tatccccttt atcaccttga
     3661 cgtccagaac gaccacgtaa ctggtcatca atacgacgag attcatgtcg ctctgtacct
     3721 attactgcta aaccgcctaa ttcctctacg ccttcaccta atttgatatc tgtaccacga
     3781 ccagccatgt tagtggcaat agtaacggca cctttttgtc cagcgcctgc aacaatttca
     3841 gcttcacgtt catgattttt cgcatttaac acatcatgac ggataccacg ttttttaagt
     3901 aaatttgaaa tatattcaga agtctcaact gcaacagtac ctaatagcac tggttgccct
     3961 gccttgtgtt tttcaacaac atcttctact actgcatcaa atttaccttt ttggctaatg
     4021 taaattaaat cagacttatc gttacgttgc acaggtttat ttgtcggaat ttgagttact
     4081 gtcatgttat aaatatttct aaattcttct tcttcagttt tagctgtacc tgtcataccc
     4141 gcaagtttat tgtacattct gaaatagttt tggaatgtaa tagacgccat agttttagat
     4201 tcattttgaa tttgaacgcc ttccttcgct tcaatagctt ggtgtaaacc ttccgagaaa
     4261 cgacggcctg gcattgtacg tcctgtaaat tgatcgacaa ttaatacttc gccatcaaca
     4321 accatatagt ctacgtcacg ttgtaatgta acgtgcgcac gtaaagctgt gttgatatga
     4381 ctaataacat caacattttg tacatcatat aagttttcaa ctttgaacat acgttcagct
     4441 ttatccgcac cttgttctgt taaatgtaca gctttcgttt tttcatcgta tttataatct
     4501 tcgtcctgtt ttaacatttt cgcaaaaaca tttgcttgtg tataaagtga cgttgacttt
     4561 tcagcttcac cagaaataat taatggcgta cgtgcctcgt cgattaaaat tgagtcaacc
     4621 tcatcaatga ttgcaaaatg taatggacgc attactctat cttcagaata attcaccatg
     4681 ttatctcgta agtaatcaaa acctagctca ttattagtac tgtaagtaat gtcttgtgcg
     4741 tatgcttcac gtttttcttc tgtcgtctta ctgtttaagt ttaatccgac agtcaaacct
     4801 aagaagttat ataactcagc catttcttca ctttgaacac ttgataagta ttcattgact
     4861 gtaataacgt gaacacctct accagctaat gcatttaagt atgttggcat tgtcgctgtt
     4921 aatgttttac cttcacctgt tctcatctca gcgatatcac ctttatgaat tgcaatacca
     4981 cccataattt gaactttata tggtgtcata ttgaatacac gtttagagcc ttctctaaca
     5041 agtgcatatg cttctggtaa aattttatct aaataatcat tttgcttttt gacattatca
     5101 atgtcagcta attctgtttg gaattgtttc gttttattac gaatttcttc atcagttaaa
     5161 attgccgttt tttcttctaa agcgattact ttatcagcaa gtttacctaa ctgtttaatt
     5221 tctttattat tgccatcaag aatttttgat aaaaatccca tttcgttcgc tcctttagct
     5281 aaaaaactgt ttggcctaca acaatatatc ttatcattta tagttagaaa attatactta
     5341 tttactcatt tgtagaatca atataaatat atttatgaca tacttcattc acattctgtt
     5401 gtcaacaagt ttatcactaa taaatatatt ctcaatacgc aattatactt cctaataaat
     5461 tatattataa atattttacg attttcgact cggactatac aatagactga catactatta
     5521 ttaacttaac attcaaatat atacatccat taacattagc atagtcacta tgtttcattc
     5581 aacaaattac attatcgaac tatgaaatag tcataatttg cttttggagt ataaaaaagc
     5641 acttgtgcaa aaacacaagt gctttaaact taatttattg ttcactagtt tgaatcaagc
     5701 catatttacc gtctttacgg cggtaaacga tacttgttcc atcagtttct ctgtctgtga
     5761 atacaaagaa gtcatgacct aatagattca tttgtaatac cgcttcttct gaatccattg
     5821 gttttaagct gaattctttt gaacgaataa tttctatctc gttatcatcg taagcgtcat
     5881 tatcaacttg tgtttcttgc atttcttgta attcggcaac aaacacttct tgatctcctc
     5941 gatcacggct cttacgatta atacgtgttt tatattttcg aacttgtctt tcaagtttat
     6001 tattaattaa atcaatacct gcgtataaat catcgtttcg ctcttcagct cttaacgtaa
     6061 catttttcaa tggaattgtt acttcaattt tagtagctga atttgaataa gttttaactt
     6121 taacatgcgc cactgcattt ggtacgtcat taaaataacg ttccaactta ccaatttttt
     6181 cctcaatata gttgcgaata gcatctgtga tagtgaggtt atctccatga atttcaaatc
     6241 taatcatagt aatctctcct taaacctctt tattggtaac tctttattat attttaacat
     6301 ttttacgcta tcgtgcaaac gcaaacactt tgaattttct gatattttta gcatataatt
     6361 tacaccctgc acgatgaatt gttaatcaag ttgtataaat atcgtcaacg agtaatattt
     6421 ccttaccatt taaatctaat tccgtatcaa taataaatgg attttcatct gccagacgct
     6481 ctttcttagt taaatgagac tgttttggtc tatttgacat ctttaaaatc ttatcaaagc
     6541 gaatcccttt agcttttagt actgcttcta ccgggttaaa tgttctagat aaatcatgtg
     6601 ccggcgaaga aggaattggc acaatatagt cataagatgt ttgtggtatt tcaatcaaat
     6661 gtgccaataa ttcacataaa taatagtctt tcaaaaattt atactgatgt atcatctctt
     6721 tcattaaacc gtcatattga aattgacaat ataattgttc cattaaatta aagtgtgccg
     6781 atagaaactt gcagtctaaa caatacgctt catcttgatt taagtgtttt aagcaccttg
     6841 aacatcgcct tgctttaata tcaagtttaa tattgtccca attctctttg catctgtcac
     6901 ataatctatt aggtttcttg aacaaattat aaatggttat attttcatat aacttagcac
     6961 cacaactcaa acaattattc atcaatccaa cctcttttta atgctaattt gttcatcctt
     7021 tgaatctctt ttttagcttg aatcatattc atacttaccc cttcatgaaa aaacaatact
     7081 tttccagtag gacattctaa ttttcgtcca acacgtccag caatttgtat taaagcctct
     7141 tgagtatatt gatgtgcatc gataacaaca acatccaaat ttgccattgt aaatccacgt
     7201 tctaatattg tcgtagtaaa aatgacatcg aaatgtccat tccttaattg ttcaactttt
     7261 tcaaagcgaa aaacatcctc gctatggacg tatgttaatt tagtaatttt ctgcttataa
     7321 accgaaaatg ttttaatcat tgtttctata ttgttaaaaa acaccagtgt ataacgttga
     7381 ttattaattt gatcttgtaa aattcggtat aacattttct gaatcttctt attattaagt
     7441 ttgaaataac gatatttagg aactggaagt gattttttat gaaagcgagc tggcaattta
     7501 attatatttt cgtggggaat ctctgacaga agttgtttcg gtggtgttgc tgtcatataa
     7561 attgttgcat gttcaacttt agaagatgac ttcaatgctt gttgtaaatt tttatccatt
     7621 gataaaggaa aggcatcgac ttcatcaata aaaatagtat caaagtgctg tttgaatcgg
     7681 taaagttgat gcactgtgca tacaacaaaa tgcccttcaa attgttgtct tgattgctgg
     7741 tgtagtatgt ctatatcttc attaagaaat gcgtctttaa tacgtttact aatttctaca
     7801 acaacatcta cacgtggtga cacaatagct atattatctc cctgtattct tgcatattga
     7861 atgccttgaa acatcatttc tgtcttacct gcacctgtta cggcatataa caaaatcgtt
     7921 tgtctctttc taatggctcg aacaatatgt tctgaggcat aagactgctg ttccgacaat
     7981 tcaaatgaga gatgataata tgcttgtgat gaactttgcg aactctctgt tattttatat
     8041 cttgttacat tatccattcg acccagtgat atacaatttc tgcaatatac aattttcttg
     8101 gaatgtactg aggaatcata ctgataaaaa tgctttggat ttgtactttc acattgaaca
     8161 caataatagt ttgaattttg tttgatgaca cctgttactt cttttttaat tttttcatta
     8221 gtaagtgaag tatgaggatg aattaatttc ccgtaatgtt taatgcttaa aacctcattt
     8281 caacgtaaaa aagagcctaa tattataatg attttcttat atctttagca ttttggcttt
     8341 caaatgatga acatttaaag ccatgtatac gagagataat ttccaattac aatatagact
     8401 cttttattaa aattttataa ttatgttaat cttatttttc tgccaacata gcctaaacct
     8461 aatccaccag aacctaaatg tgctgcaaca actggaccaa actcagagta tgctacttga
     8521 taagctgaag gacaatcatc ttgtaacttt ttgtataacg cttgaccatc ttcgaaatga
     8581 tctccattta tgacaaataa agttacttct tcaaagtctt ttacaatatc taatactttc
     8641 ttttctaatg tttgaatggc acgcttttta gtacgaactt tttcttctgg tataatcttg
     8701 ccatcttcaa acttaagaac tggcttcatt ttcaataatg ttccaaccca tgcttgagca
     8761 ccagtaattc gaccactttt ttgtaaattc tttaagtcat caacaattaa atatgcacct
     8821 gtgtgttcac gcatattagt taaatcatca ataatttgct gtggctcgta tccttcttca
     8881 accatttcaa tagcacgtaa tacataacat ccttcaatca ttgctgcaag cttactatca
     8941 aatgcatgta cattaacacc ttcaaccatt tcccctgctt gataactaga ttgataactt
     9001 ccactaatcc cacttgataa gcaaatgaca atgatatctg tgtaaccttg atctcttaac
     9061 atttcatatt tagtaatcca ttcgccaatt gctggttggc ttgttgttgg aatcgtttga
     9121 gacgatgcca ttttattata aaattcttca attgctattt cattactttc tgtaaagttc
     9181 ttcccatcat caaaagtcac acttaatggc gctatttgaa tattatattt atcgattaag
     9241 tcctgcgaca gataacttgt agaatcggtc atcacagcaa ttttcatagt cgtcctccta
     9301 aaatatactc atagcaatga tacaatattt cagttaaaaa gaaaatggca atcaaacgat
     9361 ttatagtcat gtcattttca gtgtataatg aagattaaat aatcttaagc ttatgaatta
     9421 aggagttatt atgacacaaa atatcattac tgttaaaaaa gaacacatta ttgaaaatgt
     9481 gattagtaaa tcaagattca ttgctcatat caaacctgtt caaaatgaag atgaagctaa
     9541 agcatttatt gctgcaataa aaaaagaaca taaagatgcc actcataatt gttctgcata
     9601 tactattgga cctgaaatga atattcaaaa agcgaatgat gatggtgaac cgacaggaac
     9661 tgccggtgtc ccaatgttag acatattaaa aaagctcgat gttcataatg cgtgtgtcgt
     9721 agtaacaaga tattttggtg gtattaaatt aggtggtggc ggtttaattc gcgcctatag
     9781 cggtgctgtt agagacgtca tttatgatat cgggcgtgtc gaactacgag aagcagtacc
     9841 atgtatagta acactgaatt atgatcaaac tggtaaattt gaatatgaat tagcttcaac
     9901 tcattttatg ttaagaaacc aaacttacac tgataaggta agctactata tcgatgtcgt
     9961 taaaagtgac tatgaagcgt ttattaactt tttaaatcaa atgactgcag gaaatttcga
    10021 tttgactgaa gaaactccaa agcagttacc tttcgatata ccaactgaat aaaaataaaa
    10081 agcactatca taagacggta aatatcaatt gcgtattatt aaacgacaat tgaagtacac
    10141 gtttatggca gtgcttttta tatttgaaat ttaatacaaa aaaattgtaa agagtaaagc
    10201 catataatac aaattatccc atacagctat gctttcattc cctattcctc tttatgagat
    10261 gacttacgac taattaaatt taatattggt cggtagttgt tatctattaa tcctgtaaat
    10321 tcaacaatta attcaatact aaatatgatt aatacaaaca ttaatacaac acctaatggt
    10381 ggcgatacat acaaaataat gctcgaaaga ctaaataaaa tagagattga atagattaat
    10441 aatactgttt gtctatgtgt gtagcctaaa gctaatagtt tatgatgcaa atgcgattta
    10501 tcagcttgca ttatatgctg cccttttttc acacgtcgaa tcattgcgaa caaagtatca
    10561 atgaatggaa ctgctaagat aacaattggg aagaacaatg caataattgt aatatttttg
    10621 aatccgagta aagaaaggaa tccgatgata aatccaatca ttaaagcccc actatcacct
    10681 aaaaatattt tggcaggatg gaaattgtaa aataaaaacc caattaaaga gcctaataaa
    10741 acacaacaaa tcatcgttat gaaaatatta gcttgtaaaa ttgcaatgaa ccctattgta
    10801 atgagtccga ttgcagaaac acccgacgcc aaaccatcga gtccatcaat taagttaatt
    10861 gcatttgtaa tagcaacaat ccaaatcaca gtaattggaa tactaagaaa tccaaaatga
    10921 atcgttgtac ccattggcaa cgaaataaaa tcaatagtca caccataaaa agcaactact
    10981 aaggcagcgg caatttgacc agccaatttt atatacggtt tcaaatcgta gatatcatct
    11041 acaagcccaa gtacgtacat aataatcgca ccaataataa gtggtttgat ttctgtttca
    11101 ataggatgac caatccaaat acctattaaa aatgaaaaga gaatcactgt accacccata
    11161 actgaaatag gtttagtgtg tacttttcta aaatttggtt tatcaactaa atttaatctt
    11221 ttcgatattg caataacaat tggtgttatc gtcaaactga caatcattgt tactgcaact
    11281 agtaataatg taaccattta ttcaccttca tcgatattaa ttgtttttaa cgttatattt
    11341 atttaatatt gtctttatag aatgatatat ctattttcta cgtatatctt atagctttat
    11401 tataaatt
//
LOCUS       DANJFZ010000086        11070 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.086, whole genome shotgun sequence.
ACCESSION   DANJFZ010000086 DANJFZ010000000
VERSION     DANJFZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.086"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            359..2548
                     /locus_tag="PWN45_001966"
     CDS             359..2548
                     /locus_tag="PWN45_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL0623726.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2560..3012
                     /locus_tag="PWN45_001967"
     CDS             2560..3012
                     /locus_tag="PWN45_001967"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL0623727.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            3009..3884
                     /locus_tag="PWN45_001968"
     CDS             3009..3884
                     /locus_tag="PWN45_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0623728.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4345..5607
                     /locus_tag="PWN45_001969"
     CDS             4345..5607
                     /locus_tag="PWN45_001969"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL0623729.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5623..7389
                     /gene="aspS"
                     /locus_tag="PWN45_001970"
     CDS             5623..7389
                     /gene="aspS"
                     /locus_tag="PWN45_001970"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL0623730.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7467..7657
                     /gene="ssrS"
                     /locus_tag="PWN45_001971"
     ncRNA           7467..7657
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWN45_001971"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7722..7850
                     /locus_tag="PWN45_001972"
     CDS             7722..7850
                     /locus_tag="PWN45_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623731.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            7850..8623
                     /locus_tag="PWN45_001973"
     CDS             7850..8623
                     /locus_tag="PWN45_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDL0623732.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8784..10058)
                     /locus_tag="PWN45_001974"
     CDS             complement(8784..10058)
                     /locus_tag="PWN45_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL0623733.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            10143..10565
                     /locus_tag="PWN45_001975"
     CDS             10143..10565
                     /locus_tag="PWN45_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL0623734.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10665..10847
                     /locus_tag="PWN45_001976"
     CDS             10665..10847
                     /locus_tag="PWN45_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0623735.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            10887..11033
                     /locus_tag="PWN45_001977"
     CDS             10887..11033
                     /locus_tag="PWN45_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623736.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT         3975 a         1531 c         2172 g         3392 t
ORIGIN      
        1 gttgtttaag ttttcttaac atgagatgtt aggatttttt atttactgaa aatgttagat
       61 gattgagcat tataccttaa taacatcgtt tatttatttc ataaattgta gtatcataga
      121 actaatattt aaaaaatgaa acagtagatt taggtcgaat ttttgtaaaa gttttaaaag
      181 taggaatagt atacaaatta aactcgctca agtaaaatta atattacgat taatgacgac
      241 aggataaata tttatcgtcg acggacgtat gattggtgtg ggacaaatac tattcaacaa
      301 gagtacctaa atcattgttt aaggcgaagt aataaatatg aatggggtgt atcatataat
      361 gaacaacgaa tatccatata gtgcagacga agttcttcac aaagcaaaat catatttgtc
      421 agcagatgaa tatgagtatg ttttaaaaag ctatcatatt gcttatgaag cacataaagg
      481 tcagttccga aaaaacggat taccatacat tatgcatcct atacaagttg caggtatttt
      541 aacagaaatg cgattagacg gaccgacgat tgtcgcaggt tttttgcatg atgtaattga
      601 agatacaccg tatacatttg aagatgtaaa agaaatgttc aatgaagaag ttgctcgaat
      661 tgttgatggt gtgacgaagc ttaaaaaagt aaaataccgc tcaaaagaag aacaacaagc
      721 tgaaaatcat cgcaagttat ttattgcgat tgccaaagat gtacgcgtaa ttttggtgaa
      781 attagcagac agattacata atatgcgtac cttgaaagcc atgccgcgcg aaaaacaaat
      841 tagaatttct cgagaaacat tagaaattta tgcaccatta gcacatcgtc ttggtattaa
      901 tacaatcaaa tgggaactag aagatacggc tcttcgttat attgataatg tgcaatattt
      961 tagaatagtc aatttaatga agaagaaacg tagtgaacgt gaagcgtata tcgaaacggc
     1021 tattgataga atacgtactg aaatggaccg aatgaatatc gaaggcgata taaatggtag
     1081 acctaaacat atttacagta tttatcggaa aatgatgaag cagaaaaaac aatttgatca
     1141 aatttttgat ttgttggcga tacgtgttat tgtcaattct attaatgatt gttatgcgat
     1201 acttgggttg gtgcatacgt tatggaaacc gatgccagga cgttttaaag attatattgc
     1261 aatgcctaaa caaaatttgt atcagtcatt gcatactaca gtagtaggcc caaatggaga
     1321 cccgctcgaa atccaaatac gaacgtttga tatgcacgaa attgctgagc atggtgttgc
     1381 agcacactgg gcttacaaag aaggtaaaaa agtaagtgaa aaagatcaaa cttatcaaaa
     1441 taagttaaat tggttaaaag aattagctga agcggatcat acatcgtctg acgctcaaga
     1501 atttatggaa accttaaaat atgacttaca gagtgacaaa gtatacgcat ttaccccagc
     1561 gagtgatgtt attgagttgc catatggtgc tgtgccgatt gattttgctt atgcgattca
     1621 cagtgaagta ggtaataaga tgattggtgc caaggtgaat ggcaaaattg taccaattga
     1681 ctatatttta caaacaggcg atattgttga aatacgtact agtaaacatt catatggacc
     1741 aagtcgtgat tggttgaaaa ttgttaaatc gtctagtgcc aaaggtaaaa ttaaaagttt
     1801 cttcaaaaaa caagatcgtt catctaatat tgaaaaaggc cgaatgatgg ttgaagctga
     1861 aataaaagag caaggattta gagtcgaaga tattttgaca gagaaaaata ttcaggttgt
     1921 taatgaaaaa tataactttg caaatgaaga tgatttattc gcagctgtag gatttggcgg
     1981 cgtgacatcc ttacagattg ttaataaatt aactgaaaga caacgtattt tagataaaca
     2041 acgtgcttta aatgaagcac aagaagttac gaaatcattg cctattaaag acaacatcat
     2101 tactgatagt ggtgtctatg tagaaggttt agaaaatgta cttatcaagt tgtcaaaatg
     2161 ttgtaatcct ataccaggtg atgatattgt aggttatatc accaaaggtc acggtattaa
     2221 agtacatcgc actgattgcc caaatattaa gaacgaaact gaacgactaa ttaatgttga
     2281 atgggtaaaa tcaaaagacg caactcaaaa atatcaggtt gatttagagg taactgcgta
     2341 tgaccgaaat ggcttgttga atgaagtact acaagctgtt agctcgacag ccggcaattt
     2401 aattaaagtt tcaggacgtt cagatattga taaaaatgca ataataaata ttagtgtcat
     2461 ggtgaaaaac gtgaatgatg tttatcgtgt ggtagaaaag atcaaacaac ttggtgatgt
     2521 ttatacagta acaagagttt ggaactagag gtgcaaaata tgaaagtagt tgtacaaaga
     2581 gttaaagaag catcggtgac gaatgataca ttaaataatc aaatcaaaaa aggatattgt
     2641 ttattagtcg gtatcggtca gaactctaca gagcaagatg cagatgtaat tgcaaagaaa
     2701 attgctaatg caagattatt tgaagatgac aataataaat taaactttaa tatccaacaa
     2761 atgaatggtg aaatactatc agtttcacaa tttactctct atgcagatgt aaaaaaaggt
     2821 aaccgtccag gtttctcaaa ttctaaaaat cctgatcaag cggtaaaaat ttatgagtat
     2881 tttaatgatg cgctacgagc gtatggtctt actgtgaaaa caggtgaatt tggaacacac
     2941 atgaatgtta gcataaataa tgatggtcca gtcactatta tttatgaaag tcaggacggc
     3001 aaaattcaat gaaaaaaata gaggcatggt tatctaaaaa gggtcttaaa aataaacgta
     3061 ctctaatagt agtgattgcc tttgtcttat ttatcatctt tttattttta ttgctgaata
     3121 gcaatagtga agatagtggg aacatcacga taactgaaaa tgctgaatta cgtacaggtc
     3181 caaacgctgc gtatccagtc atatataaag ttgaaaaagg tgaccatttt aaaaagattg
     3241 gtaaagtagg taaatggatt gaagttgaag atacatccag taatgaaaaa ggttggatag
     3301 ctggatggca cacaaattta gatattgtcg cggataatac gaaggagaaa aatcctttgc
     3361 aaggtaaaac aatagtgctt gatcctggtc atggaggtag tgaccagggt gcttcaagca
     3421 atactaaata taaaagttta gaaaaagatt atacgttgaa aacagcaaaa gaattgcagc
     3481 gtactttaga aaaagaaggc gcaactgtta agatgacaag aacagacgat acatatgttt
     3541 cactagaaaa tcgtgatatc aaaggcgatg cctatttgag tatacataat gatgcgttag
     3601 aatcatctaa tgcaaatgga atgacagttt attggtatca tgataatcaa agagctttag
     3661 cagatacgtt agacgctacg attcagaaga aaggtctact ttctaatcgc ggttcaagac
     3721 aagaaaatta tcaagtgtta agacaaacaa aagttcctgc tgttttatta gaattaggtt
     3781 atattagtaa cccaactgat gaaacgatga ttaaagatca attacataga caaattttag
     3841 aacaagcaat tgttgatggc cttaaaattt atttttctgc gtagggcttg caaaaatatg
     3901 tgaaagtagt tatcattgat attgaatttt ataactaaaa ccgttagtat tcttgaaatg
     3961 gtaaatgaaa taggtagcaa tctaactaag attgtgtagg aatataatcc atagactgaa
     4021 agattatgct gagtagttta tatacattga acacaagaag aggtgcttta tgaaaagtaa
     4081 agccgttaaa cgtacgttaa acgttttgag tgggtttatt aaatgcacgc ttataaaaag
     4141 taatgatgat tacaattagg catgtttttt aaaccaatga gggtggcaac acggatattc
     4201 gtcccttgtg cagtttcagg ctgtgcaagg gctttttctt ttgcttttaa gattatgatt
     4261 tatcgtgcaa agttaagtgg tcgtatatag ttttagtttt aaaaaggtaa ttaaataaaa
     4321 tagtttgccg agggagatgt caaaatgatt aaaataccta gagggacgca ggatatttta
     4381 cctgaagatt caaagaaatg gcgttacatt gaaaatcaat tagatgaatt aatgacattt
     4441 tataattata aagaaataag aacaccaatt tttgaaagta cagatctttt tgcaagaggt
     4501 gttggtgatt caaccgatgt cgtacaaaaa gaaatgtata catttaaaga taaaggcgat
     4561 agaagtatta cattaagacc tgagggaaca gctgcagttg tgcgttcata tattgaacat
     4621 aaaatgcaag gtaatccaaa ccaaccaatt aaactttatt acaatggacc gatgtttaga
     4681 tatgaacgta agcaaaaagg acgctatcgt caatttaatc aatttggtgt agaagctatt
     4741 ggtgctgaaa atcctagcgt agatgcagaa gtattagcta tggttatgca tatttatcaa
     4801 tcatttggat taaaacattt aaagcttgtt attaatagtg taggggatat ggcgtctcga
     4861 aaagaatata acgaagcgtt agtgaaacac tttgaaccag taattcatga attttgttca
     4921 gattgtcaat cacgtttgca tacaaatccg atgcgaattt tggattgtaa agtagaccgt
     4981 gataaagaag cgattaagac tgcacctaga atcactgatt tcttaaatga ggaatctaag
     5041 gcatattatg aacaagtaaa agcttattta gatgatttag gtattccata tattgaagat
     5101 cctaacttag ttcgtggatt ggattattat acacatacag catttgaatt aatgatggat
     5161 aaccctaact atgatggtgc cattacaacg ctttgtggtg gtggccgtta taatggttta
     5221 ttagaattgc tagatggtcc aagtgaaaca ggtattggtt ttgcgctaag tatagaacga
     5281 ttattgcttg cacttgaaga agaaggtatc gaattagata ttgaagaaaa cttagattta
     5341 ttcattgtta caatgggtga tcaagcagat cgatatgctg tgaagctatt aaatcatttg
     5401 agacataatg gtattaaagc agataaagac tatttacagc gtaaaattaa aggacaaatg
     5461 aaacaagcag accgtttagg tgccaagttt acaatcgtta ttggtgatca agaattagaa
     5521 aataataaaa tcgatgttaa aaatatgaca actggtgaat ctgaaacaat tgaattagac
     5581 gcattagtcg aatattttaa gaagtagaga gggcgttaaa atatgagtaa gagaacaact
     5641 tattgtggat tagttactga ggcattttta ggacaagaaa ttacattaaa aggatgggtt
     5701 aacaatcgtc gtgaccttgg tggattgatt ttcgttgatt taagagatag agaaggaatt
     5761 gtacaagtcg tgtttaatcc tgcattttca gaagaggcat tgaaaattgc tgaaacagta
     5821 cgttctgaat atgttgtaga agttcaaggt acagttacga agcgtgaccc tgaaacagtt
     5881 aatcctaaaa ttaaaactgg ccaagttgaa gtacaagtta caaatattaa agtgattaat
     5941 aaatctgaga caccaccatt ttctataaat gaagaaaatg ttaacgttga tgaaaatatt
     6001 cgattaaaat accgttattt agatttacgt cgtcaagagt tagcgcaaac atttaaaatg
     6061 agacatcaaa ttacacgttc tattcgtcaa tatttggatg atgaagggtt ctttgacatc
     6121 gaaacaccag tactaacgaa gtcaacacct gagggtgcac gtgactattt agtaccatct
     6181 cgtgttcatg atggtgaatt ttatgcatta ccacaatcac cacaattatt taagcaatta
     6241 ttgatgatta gtggatttga caaatactac caaatcgtaa aatgcttccg tgacgaagat
     6301 ttacgtgcag atcgtcaacc tgaatttaca caagtcgata ttgaaatgag ttttgtagac
     6361 caagaagatg tgatgcaaat gggtgaagaa atgcttaaaa aagttgttaa agaagttaaa
     6421 ggcgttgaaa ttaatggcgc tttcccacgc atgacatata aagaagcgat gcgtcgctat
     6481 ggttctgata aaccagatac acgttttgaa atggaattaa ttgacgtttc tcaattagga
     6541 cgtgatatgg actttaaagt atttaaagat actgttgaaa atgatggtga aattaaagca
     6601 attgtcgcta aaggtgcagc tgaacaatat actcgtaaag atatggatgc tttaacagaa
     6661 tttgtaaaca tctatggtgc taaaggatta gcgtgggtta aagttgtgga agatggtttg
     6721 acaggtccaa ttggacgttt ctttgaaaca gaaaatgttg aaacattact tacattaact
     6781 ggtgctgaag ctggtgactt agtaatgttt gttgcagaca aaccaaatgt cgttgcacaa
     6841 agtttaggtg cattacgtgt caaattagct aaagaattag gtttaatcga tgaaacaaaa
     6901 ttaaacttct tatgggtgac agattggcca ttattagaat atgatgaaga tgcgaaacgt
     6961 tacgttgcag cacatcatcc atttacatct ccaaaagaag ctgatattgc taagcttggc
     7021 actgcgccag aagaagctga ggcaaatgct tatgacatag tattaaatgg ttatgaatta
     7081 ggtggcggtt caatcagaat tcatgatggt gagttacaag aaaaaatgtt cgaagttctt
     7141 ggatttacta aagaacaagc acaagaacag ttcggcttct tactagatgc atttaaatat
     7201 ggtgcaccac cacatggcgg tattgcatta ggattagacc gattagtcat gttattgact
     7261 aatagaacaa acttgagaga tacaatcgca ttccctaaaa cagcatctgc tacatgttta
     7321 ttaacgaatg cgcctggtga agtttctgat aaacaattag aagaactttc tttgcgaatt
     7381 cgtcactaaa ttttaaaaaa tcaataaact ttggtttgtt ataatttcat taattttaat
     7441 tatgtggtaa tataacatca taagatagtc atctgagttg ttcgtaagtt tgctttttaa
     7501 tttgggccta acactctttg atcaagggag cccaatgggt tttcttgcag cgtacacgcc
     7561 ttatatgaag gacgtacaaa acaagaaaca gggcacccac ctgtatataa caggccgaat
     7621 gatcaagcta ttataactac ggcaatacgg actctatcga tacgcaagac tttgtcttgc
     7681 gtatttttat gcgtttcttt aagtttttaa taaagaatta gttgggtttt atagcgttta
     7741 ataaatgttt cctcgttgaa ttttgggaaa ataatcaaag tgataatgct aaatataaaa
     7801 ttttaagtta ctatgtactt atgtataaat cagtgaaaga ggtaaaataa tgaaacatca
     7861 attttcaaga aatgaactag cgattggaca acaaggttta gatttattga agcaacaaac
     7921 tgttgtcata cttggcgttg gaggtgttgg ttcatttgct gctgaagcat tagcaagaac
     7981 gaatattggt catattatat taatagataa agatgatgtt gatattacaa atgtaaacag
     8041 acaattacac gctttaacta caacggtagg acaaagtaaa gtttctttga tggaagaacg
     8101 tattaaatta attaatccag attgtaaagt cacgccctta catatgtttt atacagaaga
     8161 gacatatgaa gaaattttcg ataactacga tatagattat tttgtagatg caagtgatac
     8221 aataatgtac aaagtacact taatgaaaga gtgtcttgac cgagggatta aagtgatttc
     8281 cagtatgggt gctgcaaata aaacagatcc aacacgtttt acaatttcag atatctctaa
     8341 aacacatacc gatccaatgg ccaaaattat tcgtcaaaaa ttgaaaaaac taggaattcg
     8401 taaagggatt ccagttgtat tttcagatga aagtccaatt gtcataagag aagatgtaaa
     8461 agatatagtt ggagataaaa atgctatcaa tcgaaaagga caaatgccac cttcttcaaa
     8521 tgcctttgtg ccaagtgttg ttggattaat ttgtgcaagt tatgtggtga atgacgtatt
     8581 aaaagatatt ccagttcgtc gcattaaaga caaagggcaa taattcattt tgaaagggat
     8641 aattttcaac gtaaggcaag tgtaaccaca ccataaaact aaaactgact agttcgcaag
     8701 catagtagaa tatgcctcgt gtactagtca gttttgattt gattatagct aaaatatcaa
     8761 aggtaatcga tttaatctta tttttacggc ctttgtttaa gtaagttgtt atatatttct
     8821 ttgagttgtt gttcactttt agatgtcgtt tttggttcgt aataaatttt gttttttagt
     8881 ttatcaggta aatattgctg tgaaacatag ccattaacat attggtgtgg atatttgtaa
     8941 ccaatagatc ggcctagatc tttagcacct tgataatgtc catcttttaa atggtttgga
     9001 atttggccca catgaccgtt tctaatatcg gacaatgcac tatcaatggc actcattgct
     9061 gaatttgact taggtgataa gcatagttcg attactgctt ggcttagtgg aattctagct
     9121 tctggtaaac ctagacgttc tgctgattca atagcagcaa gtgttctctg accagcatta
     9181 ggtgaggcta agcctatatc ctcatagcta attacaagta atcgtcgaac tattgtaggt
     9241 aaatctccag cttcaattaa tcgtgctaaa taatgtaaag cggcattgac gtcgctacca
     9301 cggatagatt tttggaaagc gctcataaca tcgtaatgca tgtcaccatc cttgtcactt
     9361 acaaatgcac ctttttgtaa acagtcttta gcatcttgca atgtaacatg tcgataaccg
     9421 tctttgtcat tatctgcgct taatacagct aattccaatg catttaacgc actacgaaca
     9481 tcaccttggc tttgtgtaga aaagtaggtc atggcatctt catcaatttt gggttgatat
     9541 gttttcaaac cattctcatc atcttctatt gcacgagtta acgcttggcg cacatcttcg
     9601 tcatttaaag gatataactc gaaaatttgc gctcttgaac gaatcgctgg attgatagca
     9661 tgataaggat ttgaagttgt agcaccgatc aagacgattt tgccattttc taaatgaggt
     9721 aataaaaagt cttgtttagc tttatctagt cgatgtattt catctaataa caagataact
     9781 tgaccagaca ttttagcttc ttcaacaaca agttgcatat cttttttagt gttagttaca
     9841 gcattcaatt gtctgaattt atattgcgta ctgcccgaaa ttgctttggc aatacttgtt
     9901 ttgcctatac caggtggacc ataaaaaatc attgaagtta atttttttgt atcaaccatt
     9961 cttctgataa tgcctcttgg tccaactaaa tgttgttggg aaatgatttc atctatattt
    10021 tttgggcgca ttctcgatgc taatggttct gtactcacat ttgtcacacc tttcttatac
    10081 tatcttaact caaaaaatgc taaaatggga atatcaatat tcgaattaag tgaggttata
    10141 caatgaaaat ttctactaaa gggagatatg gacttacatt gatgatttct cttgctaaaa
    10201 aagaggggca aggatgtata tcattaaagt caattgctga agaaaataat ttgagtgatt
    10261 tatatttaga acagcttgta ggtcctttaa gaaatgcggg gttaattcga agtgtacgcg
    10321 gtgctaaagg tggataccaa ttaagagtgc cagcggaaga aatctcagca ggggatatta
    10381 taagactgtt agaaggtcca attacatttg ttgaaagtat tgaatcagaa ccacctgcgc
    10441 aaaaacaact atggattcgc atgagagatg cagtgagaga tgttttagat aatacaacat
    10501 tgaaatattt agcggaatat gtagatacaa gtgaagattt agacggatac atgttttata
    10561 tttaatcgta taaatactat actataacat aaaaacttca tattataatg tttagcgaac
    10621 ctccttagtg gtatataaat atatacatcc aaggaggttt cactatggca gacgaaagta
    10681 agttcgatca atttaaagga aatgttaaag aaacagtagg taacgttact gataacaaag
    10741 aattagaaaa agaaggtcag caagataaag tgattggtaa agcaaaagaa gttgttgaaa
    10801 atgctaaaaa caaaataact gatgcaattg ataaacttaa aaaataagtt caattaaact
    10861 aatcaaatat aagaggagcg attaatatga gttttatgga taaagctaaa gacgcagtag
    10921 aaaaatttaa aaatagcgat aatgaacaag ttaaaaatgt aaaagataag ataaatgaat
    10981 atacaggatc gaataacgaa gagaaaaaag aaaatgaaga taaagagaaa taaatttctt
    11041 taaattttgg atatgctttt caatattttg
//
LOCUS       DANJFZ010000087        11046 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.087, whole genome shotgun sequence.
ACCESSION   DANJFZ010000087 DANJFZ010000000
VERSION     DANJFZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.087"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(150..1994)
                     /locus_tag="PWN45_001978"
     CDS             complement(150..1994)
                     /locus_tag="PWN45_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDL0623737.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(2004..3389)
                     /gene="mgtE"
                     /locus_tag="PWN45_001979"
     CDS             complement(2004..3389)
                     /gene="mgtE"
                     /locus_tag="PWN45_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL0623738.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(3410..4264)
                     /locus_tag="PWN45_001980"
     CDS             complement(3410..4264)
                     /locus_tag="PWN45_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0623739.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(4261..5070)
                     /locus_tag="PWN45_001981"
     CDS             complement(4261..5070)
                     /locus_tag="PWN45_001981"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL0623740.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(5087..5722)
                     /locus_tag="PWN45_001982"
     CDS             complement(5087..5722)
                     /locus_tag="PWN45_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL0623741.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(5739..6086)
                     /locus_tag="PWN45_001983"
     CDS             complement(5739..6086)
                     /locus_tag="PWN45_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623742.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            6272..6865
                     /locus_tag="PWN45_001984"
     CDS             6272..6865
                     /locus_tag="PWN45_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL0623743.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            6969..7334
                     /locus_tag="PWN45_001985"
     CDS             6969..7334
                     /locus_tag="PWN45_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDL0623744.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            7357..8163
                     /gene="yjbH"
                     /locus_tag="PWN45_001986"
     CDS             7357..8163
                     /gene="yjbH"
                     /locus_tag="PWN45_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDL0623745.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(8623..10431)
                     /gene="pepF"
                     /locus_tag="PWN45_001987"
     CDS             complement(8623..10431)
                     /gene="pepF"
                     /locus_tag="PWN45_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL0623746.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(10479..>11046)
                     /locus_tag="PWN45_001988"
     CDS             complement(10479..>11046)
                     /locus_tag="PWN45_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL0623747.1"
                     /translation="NKVKFNHFQSMFINPFTRKLHTFNLEKKQIMMFQQIQYLGGHKY
                     VAEKRNAKISELFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQLT
                     DQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLKNGKSKLVNDNLNYFKLKKFIVALE
                     SKTAIIEKLINNYLNICSDKGNDVQILF"
BASE COUNT         3720 a         1940 c         1527 g         3859 t
ORIGIN      
        1 caacgataaa tgtttgattt agtcctttct gtatcaaact tgatttacgt aaaaagcatt
       61 tatttatcaa aatcatatac atactagcat tttaaaatca ttaattttac gtcgtactaa
      121 aatacgtctg cattttaata tcattattgt taaaaataaa attctaactc ttgcttcaat
      181 tcatcaacgt attctttagc accggtaaca attaagcgat ctccatatcg caattgtgta
      241 tctccatgcg gaacaatcga ctcattatta cggataatac gcacgaagat gatgtctcct
      301 ccgaatggga aattacgtaa ttgaatattt tcatatttat aatttaacat ttgaatttca
      361 tatagtgacg tttcaacatt acttaataaa ttcaacatgt taggtgtttc aattaaacct
      421 tttaataaga ttttattact taagtagcta ctgaaaattt caatacctga atcaactaac
      481 tctgtatcgt ccgttgtgct ttcaagtctg caaatgacac gctcaacttg atgtgctttg
      541 gctaatttag caacttttcg gttaatatca tcgtcattcg tagcacaaac aactatgtct
      601 cggtcaaaca gacctagtcg ttctaaaaca tcttgttcat aatcagcaat ttctatcatc
      661 gtgatatcat ctgacaattg acgacgatca ctcaagtctt tgcgataata taatgtcacg
      721 tcatataact gagatgttaa attttgcgct ataggaatcg ttaattgatt tttaccaatt
      781 aaactaactt caattttacg gttaaactca tctggaactg gaaacagttt tttgaaaata
      841 atcggaacga atacacatgt aatgactgcg cttaaaatta atatacctga cgtttcagct
      901 gaaatagcat ttaatctttc tgaaattttg gctgcagcta tcacgagcga taatgttgat
      961 gttaataaaa atgctgatgc aatcgttgtt ttcatatcaa accaacgtcg aataaacatg
     1021 actggaatta attttgaaat gataaatgca acgattaaaa tcggtatgat aattagtaat
     1081 ttcggttctt taattaatga aggtatgttt aaatctacac caaccattat aaagaaaata
     1141 ggaataaaga acccataacc aaatgagtct aacttttcaa ccatttcttc atctggattt
     1201 aataatgaaa caacgacacc tgctaagaat gcacctaata tattttctgc gccaactccc
     1261 tctgctaggg ctactaataa tattattaat gcaaataccg cacgaatacc gatttgcgtc
     1321 gtaccatcca tcaatttttg taaaaatgac attcttttaa attgaacacc taaaatatat
     1381 gaaattgctg tgaaaacaac taatatacct attaaccata ttgtactgcc gccttgacca
     1441 ttgattgcgc catagaccgt taataaaatc atagttacta agtccgcaag tactgctact
     1501 aataggataa attgccctat agttgttctc ataatattca tttcttttaa agttggaaca
     1561 actacgccta aggaaatagt tgaaatgata atgaccatta ataacacatc atccactaat
     1621 ccaagccatt taaatacata cgctaaaaga atcgaaataa tcataataaa tgcaaataca
     1681 gttaacgcta gattaagatg ccctggaatt gaggattcat catcattttg tccttgacgt
     1741 gcgcgtttat cttttttaaa agctttaaaa tcaatttcta aaccacttaa aaacattaaa
     1801 aagataaagc ctaacgttga taaaatattt agaattgaat ccctttctac tatatttaga
     1861 aatgaatttc caatcacaat ccccatcaaa atttctgcaa caacaactgg caagaaatta
     1921 atatttaatc gattgacaat tattggagtt aaaaacgctg ctaaaacaac aataactaaa
     1981 gataaaaact ccataactcc tccttaaatt aagtaagcca taaatgatgt agctaaacca
     2041 aaataaatca acatactaat aatatcatta attgttgtaa taaatggtcc actagccact
     2101 gctggatcga tatttaattt attcatcaat aatggaatca tcgaacctac aaacgtcccc
     2161 accgtcatcg cacaagttaa acttcctgca acgattaatg ctaaaagtgg ctgatgatat
     2221 attgcaacaa taattgtaaa taatattgtt gaacatacaa cacccgataa tacaccactt
     2281 cctgcttctc ttaatgcaat tctaaattta ctttgctcat taatttcccc tgtcgtaatg
     2341 ttacgaactg aaacggctaa agattgtgta cctgaatttc ctgacatacc actaataata
     2401 ggaataaacg ctgcgagtag cgctacattt tctaatgttt tttcgaatct ccctaaaatt
     2461 gtcgcagtaa tcattcctaa aaatgttaaa ataatcaacc atggtaaacg ttttaatgct
     2521 gttttaatga ttgaatcatt agtcgaatcg atatctgata ccccggctaa acgagagtag
     2581 tcttcactag cctcttcatc cataacgtct aaaatatcat caatcgtgat gataccaagc
     2641 aaatgttctt ggtaatctat aacaggtaca gccatgaaat catagtctct cataacttga
     2701 gcaacatctt cttggtcgtc tgctacattc acactaatga cacgttcatt cataatatct
     2761 tcaatataag catcattttc agctacaatt aaatctctta gcgataaaac acctactaat
     2821 ttaccatcat catcaacgac aaatataaca tatattgttt ctgcgtctgg tgcttgcgct
     2881 ttgaccaata ataatgcttc tttaacaggc gtatgcgctt taagtgataa atactccgtc
     2941 gtcataatac cgccggccgt atcctcatca tagtgaagta atgctttgat ttcattcgcg
     3001 tcatctttat tcatcaatgt taaaagacta gcaacttttg gtttagtcaa ctcatttaaa
     3061 atatctactg cattgtcgta tgacatttct tctaatatgt gacttgcgta tgtcgcattc
     3121 atcttatcaa atagcaactc atattcatcg tcatcaatat ctaattgatc aaagaaattt
     3181 gcaacttctt caggtgataa atattgaaaa atcttttgtc tattttcatc ggtagtatct
     3241 tcaaaatact cactttgttc atatgtgtgt aatgctagaa attcatctct aaattgatca
     3301 atatcatcat tttctaaata ttgatctaat aaagtttggt cgtataattc ctcttgaaca
     3361 cgctcttttt catctgtgtt cattgacata cttacacccc ctgtgtgaat tagagttgat
     3421 tgaataattt aagtaattgc ttataatcaa ttgtaatata aatattgttc ttattgattg
     3481 gatgaacaaa atatatttgc gtacattgca gtacttgacc atgaattttg tcatgaaaac
     3541 catcatacaa agaatctccc acaattggat gcccaatatg ttgaaaatgt acacgaattt
     3601 gatgtgtacg tcccgtatgc aaatgaactt tgcataaact gtatttatca ttctgattta
     3661 ttacttcata agatgtttta gcgtatttac catccgaggc aacttctcta gttataatcc
     3721 tatcctttga ccgtctaata ttagcttcaa taataccaga tgtatgggtt ttaccatata
     3781 caaggcaagt atatattttt ttcaagttta ctttagaaaa taaatgatgg atatgtccat
     3841 atttagcgaa tatcacaata ccagttgtat tacgatctag acgcgtaaca atatgtgggt
     3901 taatattttc accatgttcc tgacaatgat atagtacttg ttcgattaaa ctttcatgag
     3961 gatgttctct cgaaggcgta caattttgat tgtttggttt agtaactatg atgataaaag
     4021 catcttcata caatacttct agcttacgag cataaggtat taaattaaca ctcggtattt
     4081 ctcgcggtaa atgaatttct aatatatcat ttgtcattaa ttgcttacgc actgtgactg
     4141 gttcatcatt aacaattaaa gcgccattat ttttaatggc gctcactgtc ttcttagaaa
     4201 aatcatgtcg tgctaaaaaa gttttaacag tttcttgttg tgatatatga tacttaaatt
     4261 tcatcgttct tcatcacttg atatgaaaga atcgtgcaca cgtttccaga atgggaatgg
     4321 tctaaaacgt gcaaacctca ctttttcatt tgctacacgg tattgtattg aattaacatt
     4381 tttatgtttg atactaacat gatctatcgt cattctaatg gtatcatgat taactggtga
     4441 tattaaacat gtatgatgct ttggtaatac aagtggtgat cctaccgttc taaacacacg
     4501 attatttatc gaggcaattt ctgtaatttg cattgcttca agtgaaggat gtatcagtgc
     4561 gccacctagc gctttgttat aagccgttga acccgaaggt gttgatacac ataatccatc
     4621 gcctctaaat cgctcaaagt gtttccctct taagttaaca tccacaacaa gtgttgagcc
     4681 attttcagtt ttcatcgttg cttcatttaa tgctaaatac cttgtttcat agccgttgtc
     4741 gttgtatctc ataataattt caagcaatgg atattcaatg acctgaaact ctgaattatt
     4801 aatttcgatg attaattttt caacttcatg aggtaaccaa tccgcataaa atcctaaatg
     4861 acctgtatga actccaacaa atgccacttt tgataacatg tggctatact gatggaatgc
     4921 ttgtagtaat gtaccgtcac caccaactga aataacaatt tcaggatttt cactatcctc
     4981 aatcatgcga aaatctttca tatagttcat cattttatgc tttaaggcat tagacttgga
     5041 gtcaccttta gttaaaattg tataacgcat gatttataac acctcgttaa tcattttcat
     5101 gttttttaga acgtttttgc gtgtaatatt tttgagcttc ctgaatttca tctttaattt
     5161 cagacatctc ttcatcaagt aaatacgctg cttctgccgc tctttccaaa cgatgttgaa
     5221 tttcatccgg ataagcacca tcatatttat atcgtaaagt atgttcaatc gttgcccaga
     5281 aattcattgc taatgtacga atctgaatct cagccaatat aaatttttgg ccttgtaatg
     5341 tttcaattgg atattcaata atgacatgat acgagcggta accactttct ttagtgttac
     5401 gaatataatc tcgttcttca attactttaa aatcttttct ttgtcttaaa atattgacga
     5461 caacatcaat atcttcaaca aattggcaca tcattcttaa accagcgata tcgtacattt
     5521 cttctcttaa cctatcaaat ggtatttgtc gtttgtttgc cttatctata atactagcga
     5581 ttggtttaac acgaccagta acaaattcta ttggcgacgc ttgttcacca acttcatatt
     5641 gtttgcgcat gcctttaagt ttcactttca actcatcaac cgcttgctta taaggtgtta
     5701 agaactgatc ccattgattc atagtgcttc accccgcttt attcaatgtc gaatgtttct
     5761 tcaacggttg caacaaattc cttaccataa ttattattcc cttttatgtt gtctaaaata
     5821 taagtaagtt cttggtggaa ttcagtcaat tctagttcat cattgataat aattttttta
     5881 gtcatgtttt catgcaacat cgtccatgtt tcttgtacga aaatgagata agcatacgct
     5941 ttaccatcat ctaatgtata cgcaaaggca taattatcac tatcaacgag catttgtcct
     6001 acttcagata atcctttaat agaatcttct gtataacaat aaagtatgtc atctttaatt
     6061 ttaatttcat taatatataa acgcatgctt aatcctcctc ttattccata acattttaac
     6121 acaattcaat aatacaaaac acttacgaac actattttta atggtcaaat taacattgat
     6181 aatagataaa ggaaattact tttaaaattc aactttgtca ttcataatat agttaagata
     6241 aattgaattt gatacgaggt gtatacatat catggcaaca aatcatgaaa tagaatttaa
     6301 acaaatgata actgcatcaa tctataataa attgcaagag aaatatttta aagacagtgt
     6361 gctttttaag caagtcaatt actatattga tacacccgat ttcaaattaa aggaacatcg
     6421 ctctgcacta cgcataagag ttaaagacaa tcaatatgaa atgactttaa agacaccagc
     6481 aaaggttggt ctattagaat acaactatat cgtcgacatt aaaccagaga tgaacttgat
     6541 tatttcaaac gacaatcttc cagatgacat tcgtcaaatt atagttgaac agtttggtgt
     6601 aaaagataca actttatcaa tacttggtgc attaacgaca tatcgccaag aaactaaata
     6661 taaaggtgat ttactggttt tagataaaag tgagtatttc gatactacgg attatgagtt
     6721 agagttcgaa gttaaagact ataatcaagg tttacaaaag ttccaatctt tattaaacga
     6781 actaaacctc gaacatcatc aacctttaaa taaagttcaa cgttttttca agaaaaaaga
     6841 aacactttca aacaatataa attaaattgg caaatatgca tatttctttc aaatgtttaa
     6901 aatcatgtta tattagttat atattaactt aagttcgtaa cagtaaattt ttgaaaatgg
     6961 gtgattaaat gacaacaaca ccatatgaca tcattggtaa agaagcgtta tacgatatga
     7021 ttcattattt ttacaccctt gtagaaaaag atgaacgact taatcacctg tttccaggag
     7081 attttgcaga aacaagtcgt aaacaaaaac aatttttaac acagtttttg ggcggtccaa
     7141 acatttatac cgaagaacac ggacatccta tgctaagaaa aagacatata gattttacaa
     7201 tcactgaatt tgaaagagat gcatggttag aaaatatgca gacagcaatt aatcgcgctg
     7261 cgtttccaca aggtgttgga gattatttat ttgagcgctt aagattaact gctaatcata
     7321 tggtgaattc ctaaaattaa ttgtaggtga ataaacatgg ctggagaatt acgaataatg
     7381 gaaaataaga gtcgtgaaga tatcaatcta tcacctgtaa gtaaaatcga aatttattca
     7441 tttttcgatc catttagctc cgattgcttc aaattatcag caatcttatc caaattaaga
     7501 attgaatata atcaatatat acgtatcaga catatattaa atccttcgtt aaaagtatta
     7561 acgaaatgcc aagctcaaag tacatccaac tttgataaca tcgccctagc ttataaagca
     7621 gctgagttac aaggtcgtgt acgtgccgaa cgatttatac atttaatgca aaatgaaatc
     7681 atacctaaaa gagatattat tacagaatca atgatttgtg actgtattca aaatgcaggt
     7741 attgatttag aagtatttaa agacgactta caaaaaagta aactaaccga aagcttgaaa
     7801 attgatttgc atattgcaag agaaatggaa atcgaacaag ccccttctct cgttttcttt
     7861 agtgaagatg ttcatgaaga aggtttaaaa gtcgaaggat tatacccata tcacatctat
     7921 acttatataa ttaatgaatt gatgggtaaa cctatcgaaa agaatcttcc tcctaaatta
     7981 gaaacttata tacagcaaca acaacttgta acgatggaag aattacttac tatttatgaa
     8041 tggccagaaa aacttttaaa caaagagtta aagaagttag ccattcaaca aaaaattgaa
     8101 aaacttaaat atccagatgg cgatttctgg aaatctaaaa tgcctaaaat caaatcaaaa
     8161 taaataaatt aaagacctcg tacaccataa atgtaacgag gtcttcttac tgactcttaa
     8221 ttttgcaaca ttaaattatt agaacgtaat gttacacatc gatctaaata tattagtgat
     8281 atttttcgaa tttaaccagt tcgaaaatta tacaaattat aaatttggta cataatagac
     8341 atatatgaat cgttaatgta taaaaagtgc atatttaata catttttata caacttcact
     8401 tccctcttat taaaaaagaa cataaaaaaa cgtcgtgtct gaatacacga cgctaaacaa
     8461 aggggatggg agaaattttt cacttcaaac aaaggggtat gtttgttatg tgattaattt
     8521 catgttcata atataacatg ctatctctac catttcaacc ttttaatagt acttaattta
     8581 aattgtcaca atctgttcat aatgtaagcg aatacaatag tactaagctt tcattaattt
     8641 ttcaaaagcg ttcaattttt gttcaaaaac ttcacaagct tgttcaattg gttcaggtgt
     8701 tgtcatatct acaccagcat tttttaatat ctcaattgga taatttgagc tacctttttt
     8761 caagaattca ttaatatatc tatctactgc tggcttacct tctgttaaaa tttgatgact
     8821 taagctttga gctgcactgt aaccagttgc gtattgatat acataataat tcatatagaa
     8881 gtgtggaata cgtgaccatt ccttactaat atcttcatca gtttctacag aatcaccgaa
     8941 gtataattta tttaatttgg catattcttc attcattcta gttggcgtta atggttcacc
     9001 tgcttcttca attgcatgaa ttttatgctc aaattctgcg aacattgttt gtcggaataa
     9061 tgtagctctg aaacgttcta attcttggtt taataataat aagcgttttt catcatcaag
     9121 atgtttatcc atataatcac ttaaaagtgc ttcgttacaa gttgatgcaa cttcagcgac
     9181 aaaaatagtg tagtcactag aatttgacgg ttggaatttt ctactgaagt aactatgtgc
     9241 tgaatgccca aattcatgaa ctaatgtgta taagtctgaa atagtattag accagttaag
     9301 tagaataaat ggattagtta aatgtgcacc tgatgaatag ccacctgaac gtttaccttt
     9361 attctcatag acatcgaccc aacgattgtt taagccttct ttaactacat ttaaatattc
     9421 ttcacccatt ggttctaatg cttttaacat ccactcttta gcttcttcat aaggcatttc
     9481 aaacttaata tctttaatta atggtgtata taaatcatac attttcaagt catctaaacc
     9541 tagcaattct ttgcgcaatt cagtatatct atgtagcaat ggtaaatatt tatgtacagt
     9601 ttttactaga ttgtcatata cattttctgg aatatgatta ttactcaatg ctttttctct
     9661 tgcagttttg taattgtgtg tacgagcatt aaatacattt ttcttcactt cacctgctag
     9721 cgtagcgcca agcgtattat tatgagcacc atatgcttta tatacatttc taaaagcact
     9781 ttctcttagt ttgcgatcat ctgtttctaa atacttaata aatgtacctt gtgttaacgg
     9841 gtgtgcattt ccatctttat ctatcgcatc ttcaaatact aaatcagcgt tgctaaacat
     9901 accgtataca tttgatggcg ttgataacgc gtcctgcgct tctgttaata acttttcagt
     9961 ttcagcatct aaaatatgag gacgtttttc attaatcaac ttcaaatcaa atgcgaattt
    10021 ttgtaattta tcatatgaat ttacaaatga ttgaatttta tcttcatcaa tttgtaaaat
    10081 ctctggcact aagaaactcc atgccgagct aaatttaata attaattgat gtgctcttga
    10141 ctccatacca gtatacttgt cgttcgttgt atcttggtct tgttttaggt gcgcgtatac
    10201 atatactttt tctaatttag tacctaatgt atcttctaat tctaacgcat tgtataatgt
    10261 ctcagcacta tcaccaatgt gtcctttaaa ttgttcttct ttgcctaact cattttcaac
    10321 ttctttaaat gcagcctcaa aagcttcatc atctttgaaa attgttgtta agtcccatgt
    10381 atattcagga tatttacgtt cctgttcttc tctcgataat tgttgactca tatgtagtaa
    10441 acctcctaat aaaatataat tgatataatt ttctcatttt agaacaaaat ttgcacgtca
    10501 ttacctttat ctgaacaaat atttaaataa ttgttaataa gtttttctat aattgctgtt
    10561 ttactttcta gagcaacaat aaattttttc agtttaaaat aattaagatt gtcatttact
    10621 aatttgcttt ttccattctt taaccataaa tcaacttgta attgccactc aataggatga
    10681 tttttaatat aaatttgctc tggaaaaata taaccataat tgtgcactac ttcttgatca
    10741 gttaactgta attgatacat tgcacttaaa gtgggttcta aaacagaatt ttgccagcga
    10801 caatatttga tatattgatt aattgcgaac tttgataatt tataaacagc atgataatca
    10861 caaggcgcct cattaaacaa ctcactaatt ttggcatttc ttttttcagc gacatatttg
    10921 tgcccgccta aatattgtat ttgttgaaac atcattattt gttttttctc taaattgaac
    10981 gtatgaagtt ttcgagtgaa tggattaata aacatacttt gaaaatgatt gaacttcact
    11041 ttatta
//
LOCUS       DANJFZ010000088        10764 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.088, whole genome shotgun sequence.
ACCESSION   DANJFZ010000088 DANJFZ010000000
VERSION     DANJFZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.088"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            65..991
                     /locus_tag="PWN45_001989"
     CDS             65..991
                     /locus_tag="PWN45_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623748.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            991..2061
                     /locus_tag="PWN45_001990"
     CDS             991..2061
                     /locus_tag="PWN45_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623749.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2078..3160
                     /locus_tag="PWN45_001991"
     CDS             2078..3160
                     /locus_tag="PWN45_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623750.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3150..4091
                     /locus_tag="PWN45_001992"
     CDS             3150..4091
                     /locus_tag="PWN45_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0623751.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            4110..5765
                     /locus_tag="PWN45_001993"
     CDS             4110..5765
                     /locus_tag="PWN45_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0623752.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5977..7692
                     /locus_tag="PWN45_001994"
     CDS             5977..7692
                     /locus_tag="PWN45_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0623753.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7743..8729
                     /locus_tag="PWN45_001995"
     CDS             7743..8729
                     /locus_tag="PWN45_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623754.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8732..9712
                     /locus_tag="PWN45_001996"
     CDS             8732..9712
                     /locus_tag="PWN45_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL0623755.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            9705..10667
                     /locus_tag="PWN45_001997"
     CDS             9705..10667
                     /locus_tag="PWN45_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623756.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT         3983 a         1471 c         2002 g         3308 t
ORIGIN      
        1 atttcagaaa attttgaagg gaatggaaat ttagaaatcg gaatttgtta gaggagggga
       61 ttagatgggg aaatatattt tcaaacgatt tatttatatg cttatttctt tatttattat
      121 tattacaatt acatttttct taatgaaatt aatgccaggt tcgccattta acgatgctaa
      181 attaaatgct gaacaaaaag aaattttaaa tgaaaaatat ggattaaatg atcctgtagc
      241 tacgcagtat ttacattatt taaaaaatgt tgttacaggc gattttggta attcattcca
      301 gtatcataat caacctgtgt gggatttgat taaaccgaga ctactacctt cttttgaaat
      361 gggtcttaca gcaatgttca tcggtgtgat actgggactt attttaggtg ttgcagcagc
      421 tactaaacaa aattcttggg ttgactatac aactacagtt atttcagtta ttgcagtatc
      481 tgtaccatct tttgtacttg ctgtactttt acaatatgta tttgcagtta aattaagatg
      541 gttcccagta gctggatggg aaggtttttc gaccgcggta ttaccgtcac ttgcattatc
      601 tgcagctgtt ttagcaactg tcgccagata cataagagca gagatgatag aggtattaag
      661 ttcagactat attttattag cgagagctaa aggtaattcg acaatgcgtg tactttttgg
      721 acatgcactt agaaatgctt taattccaat tattacaatt atcgttccca tgttagcaag
      781 tattttaaca ggcactttaa caattgaaaa tatttttgga gttcctggat taggggatca
      841 attcgtacgt tcaattacaa caaatgattt ctcagtaatc atggcaatca cactattatt
      901 tagcacactg tttatcgttt ctatttttat tgtagatatt ttgtacggtg tgatagatcc
      961 acgaattcgt gttcaaggag gtaaaaaata atggctgaaa ataaaaacaa tttgtcgatt
     1021 aacgacgatc attctaatgc agctatgacg catacctctg acgctatcgc atcatctgat
     1081 tttattatta gagaattaga tttgaatcag gaacctgaaa tgcaacgaga aagcaaaaac
     1141 ttttggcaag atgcttgggc tcagttaaaa cgaaataagt tagctgttgt cggtatgata
     1201 ggtttaatta tcattgtaat atttgctttt atcggtccag ttataaataa acatgattat
     1261 gctgaacaaa atgtagaaca tagaaatctt ccggcaaaaa tacctgtatt agacaaagtt
     1321 ccatttttac cttttgatgg taaagatgca gatggcaagg atgcttataa agcagcaaat
     1381 gctaaagaaa attattggtt tggtactgat cagttgggtc gagatttatg gacaagaaca
     1441 tggaaaggtg ctcaaatttc attgtttatc ggtgttgttg cagcgatgtt agatattttt
     1501 attggtgttg tatatggtgc gatttctgga ttcttcggtg gacgtgtcga tacgattatg
     1561 caacgtatac ttgaagtcat agcatctatt ccgaatttaa ttgtcgtaat tttatttgta
     1621 ttaatttttg aaccatccat ttggacaatt atattggcta tgtctatcac aggctggtta
     1681 ggcatgagca gagttgtacg tggagaattt ttaaaattaa aaaatcaaga gtttgtcatg
     1741 gcttcgaaaa cattgggggc ttcaaaattc aaattgatat ttaagcatat tttacctaat
     1801 acattaggtg ctatcgtggt tacatcaatg tttacagtac ctagtgctat tttcttcgaa
     1861 gcatttttaa gtttcattgg tataggtgta cccgcacctc aaacatcgtt agggtcatta
     1921 gtaaatgatg ggcgcgcaat gttattaatt tatccacatg aattatttat accagcaatg
     1981 attttaagtt tattaattct attcttttac ttatttagtg atggattacg tgatgcattt
     2041 gatccgaaaa tgcgtaaata aaaagggggc atagcatatg actgaaagaa tattagaagt
     2101 aaatgatttg catgtttcct ttgatattac agcaggggaa gtgcaggcag tgagaggcgt
     2161 agatttttat ttgaacaaag gggaaacatt ggcaattgtt ggtgaatcag gttcaggtaa
     2221 atctgtaaca acaaaagcaa ttacaaaatt attccaaggg gacacaggaa gaattaaaaa
     2281 gggagaaatt ttatttttag gggaagattt agcaaaaaaa cctgaaaatg agttgattaa
     2341 attacgtggc aaagatattt caatgatctt tcaagatcca atgacatctt taaacccaac
     2401 gatgcaaatt ggtaaacaag tcatggaacc attaattaag cacaaaaatt atagtaaagc
     2461 acaagctaaa aagcgcgcat tggaaatact aaatcttgta ggtttaccaa atgcagaaaa
     2521 aagatttaaa gcatatcctc atcaattttc aggtggacaa aggcaaagaa ttgttattgc
     2581 aaccgcatta gcttgtgaac ctaaagtgct cattgctgat gaaccaacga ctgcattaga
     2641 cgtaacgatg caggcacaaa ttttagattt aatgaaagaa ctacaacaaa aaatcgatac
     2701 agcaattatt tttataacgc atgatttagg ggttgttgcg aatattgctg atagagtggc
     2761 agttatgtat ggtggtcaaa tggttgaaac aggagatgtt aacgaaatat tttatgatcc
     2821 aaagcatcca tatacatggg gattattatc gtcaatgcct gatttatcaa caacaaatga
     2881 cacaccatta ctagcgattc ctggagcgcc acctgattta ttacacccac ctaaaggtga
     2941 tgcatttgcg agacgtagtc aatatgcatt agatattgat tttaaagtag aaccaccgtg
     3001 gtttaaagtt tcaccgacac attttgtgaa atcttggtta ttagacgcac gtgcaccaaa
     3061 agttgaacta cccgagctgg taaaacaacg tatgaaaccg atgcctaata attatgaaaa
     3121 accactcaag gtagaaaggg tgtcgttcaa tgaaaaatga tgaagtgcta ttatctatta
     3181 aaaatttaaa gcaatatttt aacgcaggaa agaaaaacga agtgagagcg attgaaaata
     3241 tttcgtttga tatatacaaa ggggaaacat taggtttagt aggagaatcg gggtgtggta
     3301 aatctacaac tggtaaatca attattaaac ttaatgatat tacaagtgga gaaattttgt
     3361 atgagggtat tgatatacaa aagattcgta aacgtaaaga tttgcttaaa tttaataaaa
     3421 agatacagat gatttttcaa gacccatatg cgtctttaaa tcctaggtta aaagtaatgg
     3481 atatagtagc tgaaggtatt gatatccatc atttagcaac tgataagcgt gaccgaaaaa
     3541 aacgtgtcta tgatttactt gaaactgttg gattaagtaa agaacatgcc aatcgctatc
     3601 ctcatgaatt ttcaggtgga caacgccaac gtattggaat tgcccgtgca ttagccgttg
     3661 aaccagaatt cattatcgcg gacgaaccaa tatcggcatt ggatgtttca atccaagctc
     3721 aagtagttaa tttattatta aaattacaac gtgaaagagg gattacgttc ctatttatag
     3781 ctcatgatct atcaatggtg aagtatattt cagatcgtat tgcagtcatg cattttggga
     3841 aaatagttga aattggaccg gcagaagaaa tttatcaaaa tccattacac gattatacta
     3901 agtctttatt atcagccatt ccacaacctg atcctgaatc agaacgcagt cgcaaacgat
     3961 ttagttatat tgatgatgaa gcaaataatc atttaagaca attacatgaa attagaccga
     4021 atcactttgt ctttagtact gaagaagaag cggcacaact acgagaaaat aaattggtga
     4081 cacaaaatta aggggaaggg ggaaatgcaa tgacgagaaa atttagaaca cttattttaa
     4141 ttttgattgc tacaattgca ttaagtggtt gtgctaatga cgatggtatt tattcagata
     4201 aaggtcaagt attcagaaaa attttgtcat cagacttaac atcccttgat acatcattaa
     4261 taacggatga aatatcttct gaagtgactg cgcaaacatt cgaaggttta tacacattag
     4321 gaaaaggtga caaaccggtg ttaggtgttg cgaaagcttt tcctgaaaag agtaaagatg
     4381 gtaaaacttt aaaggttaaa ttaagaagcg atgctaaatg gagcaatggt gacaaagtga
     4441 ctgcacaaga ctttgtttat gcttggagaa aaacagttga ccctaaaaca ggttctgaat
     4501 ttgcatacat tatgggggac attaaaaatg cgagtgatat tagtactggt aagaaacctg
     4561 tagagcaatt aggtatcaaa gcattaaatg atgaaacatt acaaattgaa ttagaaaagc
     4621 cggttccata tattaatcaa ttattagcac ttaatacctt tgcacctcaa aatgaaaaag
     4681 ttgccaaaaa atatggtaaa aattacggta cggcagctga tagagcggta tacaatggtc
     4741 catttaaagt tgatgattgg aaacaagaag ataaaacctt actatctaaa aatcagtatt
     4801 attgggataa aaagaatgta aaattagata aagtgaatta taaagttatt aaagacttac
     4861 aagccggtgc atcattgtat gatactgaat cagtagatga cgcagttatt actgcagatc
     4921 aagtaaataa atataaagac aacaaaggat taaactttgt gttaacgact gggacatttt
     4981 ttgtaaaaat gaatgaaaaa caatatcctg attttaaaaa caaaaattta agattggcta
     5041 tcgcacaagc aatagataaa aaaggatacg ttgattcagt gaaaaacaat ggctcaattc
     5101 cttccgatac actaacagcc aaaggaattg cgaaagcgcc taatggcaaa gattatgcga
     5161 gtaccatgaa ttcgccttta aaatataatc ctaaagaagc aagagcgcac tgggacaaag
     5221 ctaaaaaaga gttaggtaaa aatgaagtga cattttcaat gaacacagaa gatacaccag
     5281 atgcaaaaat atctgctgaa tatatcaaat cgcaagttga gaaaaattta ccaggagtta
     5341 ctttgaaaat taagcaatta ccgtttaaac aaagagtatc actagaactg agtaacaatt
     5401 ttgaagcatc acttagtggt tggtctgcag attaccctga tcctatggct tatttagaaa
     5461 caatgaccac aggtagcgca caaaataata cagactgggg taataaagaa tatgatcaat
     5521 tacttaaagt agcaagaacc aaattggcac ttcaaccgaa cgaacgatat gaaaacttga
     5581 aaaaagcaga agaaatgttc ctaggagatg caccggtagc accaatttat caaaaaggtg
     5641 ttgcacattt aacaaatcct caagtaaaag gattaattta ccataaattt ggtccaaata
     5701 actcacttaa acatgtatat attgataaat cgatagataa agaaacaggt aagaagaaaa
     5761 aataatatgc tttgtaaatt aggctggaga catatctcca gtctttttgt gttggataaa
     5821 aactttggga ataaaaattt aaaataagtc gttttttaaa ttactgaaat tgattaaatg
     5881 cataaataac tgaatattct aaaaataaac ttgtaataat tttttctatg agtaaactaa
     5941 aaagaaaaaa ttagattgaa agtaggaggc atatgtatgg ggaagctaat taaatatatt
     6001 tcaatacttc ttattgtcgt tttagtgttg agtgcttgcg gaaaaagcag taataaagat
     6061 gaaggagtaa aagatgctac taaaacggaa acctcaaaac ataaaggtgg taccttaaat
     6121 gtagcattaa cagcaccgcc aagtggtgtt tattcttcgt tattaaatag tacacatgca
     6181 gattctgtag ttgagggata ttttaacgaa agcttattag caactgataa aaaaatacgt
     6241 cctaaggcat atattgcttc atggaaggac atcgagccgg ctaagaaaat agaatttaaa
     6301 attaaaaaag gtattaaatg gcatgatggt aatgaattga aaattgatga ttggatttat
     6361 tcaattgaag tcttagctaa caaggactac gaaggtgctt attatccaag tgtagaaaat
     6421 atccaaggtg cgaaagatta tcatgaagga aaaactgatc atattagcgg attgaagaaa
     6481 atagatgact acactatgca ggttacattt gataaaaaac aagaaaatta cttaacagga
     6541 tttattactg gacctttatt aagtaaaaaa tatttatcag atgtaccaat taaagattta
     6601 gcgaaatcag ataaaatccg aaaatatcct attggtattg gaccgtataa agttaagaaa
     6661 atcgttccag gtgaggctgt tcaactcgtt aaatttgatg attattggca aggtaagcct
     6721 gcactagaca aaatcaattt aaaagttatt gatcaagcgc aaattattaa ggcaatggaa
     6781 aaaggcgata ttgatgttgc gaatgatgct accggtgcaa tggcaaaaga tgctaagtca
     6841 tctaatgctg gtctcaaggt attatctgcg ccaagcttag actacggttt aataggattc
     6901 gtatctcatg attacgataa aaaagctaat aaaactggta aagtgagacc aaaatatgaa
     6961 gacaaagaat tacgtaaagc aatgctttat gcaattgata gagaaaaatg gatcaaagcg
     7021 tttttcaatg gttacgctag tgaaatcaat agttttgtac catctatgca ttggatagca
     7081 gccaatccta aggacctaaa tgattacaaa tatgatcctg aaaaagctaa aaaaatctta
     7141 gataagttag gttataaaga tagagatggt gacggattta gagaagatcc taaaggtaat
     7201 aaatttgaga ttaactttaa acattattca ggttctaatc ctacttttga accaagaact
     7261 gctgcgataa aagatttctg ggaaaaagtt ggcttgaaaa caaatgtgaa gttagtagaa
     7321 ttcggtaaat ataatgaaga cttagcaaat gcatctaaag atatggaagt gtacttcaga
     7381 tcatgggcag gaggtacaga tccagatcca tcagatttat accacactga tagacctcaa
     7441 aatgaaatga gaacagtttt accaaaatca gatcaatatt tagatgatgc attagacttc
     7501 gaaaaagtag gcattgatga aaagaaacgt aaagatattt atgttaaatg gcaaaaatat
     7561 atgaatgatg agttacctgg attaccaatg ttccaaggta aatcgataac tattgttaac
     7621 gataaagtac gaaacttaga cattgaaatt ggaactgatc aaagtttata taatttaact
     7681 aaagaagctt agtaatgtaa cctcgacgat tttgtcgagg tttacttata tggggggatg
     7741 ttatgaataa tgtattgtta gaggttaaag atttagaaac atcattaaaa ataaataatg
     7801 aatggttagc aactgttgaa aatatttctt ttgaattatc taaaggagaa gttttgggta
     7861 tagtagggga atctggttgc ggtaagtcca tattaagtaa gtcaattatt aaattattac
     7921 cagaaaagat atctaaacta agtaatggag aagttatatt tgatggtaaa cgaatcgata
     7981 cgctcaatga gaagcaattg ccagatattc gaggaaatga tattgctatg atttttcaag
     8041 aacctatgac tgctttaaat cctgtattta ccataaaaaa tcaacttgtg gaatctataa
     8101 aatcacataa aaaaatttct aaaaaagaag caaataaatt agcaaaagat ttactaaaaa
     8161 aagttggaat tgctagacaa gatgaaatat taaatagcta tcctcatcaa ttatctggtg
     8221 gtatgagaca aagagtaatg attgcaatgg ccatttcatg ttctcctaaa ttattaattg
     8281 ctgatgaacc tacaacagca ttggatgtca cgattcaagc gcaaatatta gacttattaa
     8341 aagaattgca aaaggaaacg caaatggcaa ttatgatgat tacacatgat ttgagtgtag
     8401 ttgctgagtt ttgcgataaa gtcttagtta tgtatgcagg tcaaattgta gaatttggag
     8461 gcataaaaga aatactacac aatccgaaac atccttatac ccaaaaatta ttatcaacaa
     8521 ttccaaaact taaagaagag cagaaacgac ttgaaacgat agaaggaatt gtgccatcaa
     8581 tccaagcatt tcacgttaat aagtgcagat ttgcaaatag atgtaacaaa aaactggata
     8641 tttgtaataa tcaatctcct aaaatgcatg tttgtgaaga tgtcattgta cgttgtcatt
     8701 tgtacaaaaa tgaatataag gagatataag aatggaaaat attttagaag tcaaccaaat
     8761 aaaaaaatac tacaaaatta aaactggatt attacaaaaa actcagtacg ttaaagctgt
     8821 tgatgacgta tcgttttcaa taaaaaaagg acaaactttt ggattagtag gagaatcggg
     8881 ttgtggtaag tcaacgttag gtaaagtgat tatcaggctt gaagatgcaa cttcaggctc
     8941 aataattgtt aatggtgaag atataacaag attacaaggt aaaaaactca gaaaatcacg
     9001 acaacaatat cagatgatat ttcaagatcc gtatgcatca ttgaatccga tgcaaatggt
     9061 tggagatatc atttcagaac ctattttaaa ttataaaaaa ttgccaaaag aagaaataaa
     9121 aaaagaagta ctatatttat taaaatgtgt tggcctaagt gaagatgcat attataaata
     9181 tgcacatgaa ttttcaggtg gacagagaca aagagtggga attgcaagag cattggcttt
     9241 gcgtccgagt ttaattgttg ctgatgagcc tgtaagtgca ttagatgtat ctgttcaatc
     9301 tcaagtactg aatttattaa aagatttaca agaacaattt aacttaagct atttatttat
     9361 cgcacatgat ttaagtgtag taaaacatat aagtgatgtc attggagtta tgtatttagg
     9421 tcatatagtt gaaatcgcat ctgataaaga aatttatgaa aatcccaaac atccatatac
     9481 aaaagcgttg atttcatcaa taccacaaat tgataaacat aataacaata gaattatatt
     9541 aaaaggagaa ttaccttcgc caagtaatcc gccgcaaggt tgtccttttc atacaagatg
     9601 tccaattgct caagatatgt gcaaaaaaag tatgccagaa ttaaaggaca ttggtaatga
     9661 acatcaagtt gcttgtttct acgtagataa agtaggtgat ttaaatgatt aaattaatac
     9721 ttaaaagatt agggttaatg atcccgttac taattttaat ctcaatagtt gtattttcat
     9781 tagcaatcat acaaccagga gatccatttt cagatctaca aaacggtaaa ataaaacaag
     9841 aagcaataaa tgcacaaagg gaaaaattag gtctcaacga ctcaatacca catcaataca
     9901 ttagatgggt caatcatgtt atacacggtg atttaggtga gtcaatcaaa tataaaaggc
     9961 cggtaattga tgttattgag gaaagaattc caaatacaat attactcggt gctatgtcat
    10021 taattattac ttatattatc tcatttgctt taggaataac gtcaggtaga tattcttaca
    10081 gtttgacgga ttatactgtg caaatattta attatttgat gttagccatt ccatctttta
    10141 ttgcgggagt atttgcaatt tttatttttt cttttgaatt acaatggttc ccgtttcaag
    10201 gttctgttga tattaacctt aaagaaggta cttttgaata ttatatgagt aaaatatatc
    10261 acacattttt acctgcattc actttaggtt tattatctac tgctggttat attcaatatt
    10321 tacgtaatga tattattgaa aattctaaaa aagattatgt attgacggca aggtcaaaag
    10381 gattatctat gaataaaatt tataataaac atatattgag aaattctcta atacctatta
    10441 ttacattttt aggtgctgat attgtaagta ttttaggtgg agccgtgatt acggagacta
    10501 tcttttcata taacggtatc ggtaaattat ttttagaatc ggtaataggc caagactatc
    10561 cattaatgat ggcattaacg ttatttttct catttttagg tttactgggt aatttgattt
    10621 ctgatattac ttatggattt atagatccaa gaattagaag taactagggg ggattagaat
    10681 gcaaaataag tcaaaatcgc cttttaaaat tgcattttct aaatttattc ataataaaat
    10741 tgcaatgtta tcggttattt tttt
//
LOCUS       DANJFZ010000089        10754 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.089, whole genome shotgun sequence.
ACCESSION   DANJFZ010000089 DANJFZ010000000
VERSION     DANJFZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.089"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            37..1080
                     /locus_tag="PWN45_001998"
     CDS             37..1080
                     /locus_tag="PWN45_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0623757.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            1070..1699
                     /gene="vraR"
                     /locus_tag="PWN45_001999"
     CDS             1070..1699
                     /gene="vraR"
                     /locus_tag="PWN45_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDL0623758.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(1815..3032)
                     /locus_tag="PWN45_002000"
     CDS             complement(1815..3032)
                     /locus_tag="PWN45_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL0623759.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(3310..3585)
                     /locus_tag="PWN45_002001"
     CDS             complement(3310..3585)
                     /locus_tag="PWN45_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0623760.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(3592..4056)
                     /locus_tag="PWN45_002002"
     CDS             complement(3592..4056)
                     /locus_tag="PWN45_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL0623761.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            4203..4403
                     /locus_tag="PWN45_002003"
     CDS             4203..4403
                     /locus_tag="PWN45_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDL0623762.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            4406..5662
                     /locus_tag="PWN45_002004"
     CDS             4406..5662
                     /locus_tag="PWN45_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL0623763.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            5752..6282
                     /locus_tag="PWN45_002005"
     CDS             5752..6282
                     /locus_tag="PWN45_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0623764.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(6542..7693)
                     /gene="yfkAB"
                     /locus_tag="PWN45_002006"
     CDS             complement(6542..7693)
                     /gene="yfkAB"
                     /locus_tag="PWN45_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDL0623765.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            7917..8078
                     /locus_tag="PWN45_002007"
     CDS             7917..8078
                     /locus_tag="PWN45_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623766.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            8209..8724
                     /locus_tag="PWN45_002008"
     CDS             8209..8724
                     /locus_tag="PWN45_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL0623767.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            9065..9874
                     /gene="sgtB"
                     /locus_tag="PWN45_002009"
     CDS             9065..9874
                     /gene="sgtB"
                     /locus_tag="PWN45_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDL0623768.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            10135..>10754
                     /gene="recX"
                     /locus_tag="PWN45_002010"
     CDS             10135..>10754
                     /gene="recX"
                     /locus_tag="PWN45_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL0623769.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFT"
BASE COUNT         3860 a         1555 c         1860 g         3479 t
ORIGIN      
        1 atcaacgttt atcggagacg tagaggtgat ttatcgatga accactacat tagaacaatt
       61 ggttcaatgc tcatcttagt atatagcatg ctagctgcat ttctgttcat cgataaagtt
      121 tttgtaaata tcatctattt tcaaggtatg ttttatacac aaatattcgg aataccagtt
      181 tttttatttt taaatctcat catcatatta ttgtgtatta ttgttggttc ggtactcgct
      241 tacaaaatca atcagcaaaa tgattggatt aagacgcaaa ttgagcgttc aatggaaggc
      301 gaaacagttg gcattaatga tcaaaatata gaaatatata gtgaaacgtt agatttatac
      361 catacactcg tacctttaaa tcaagaattg cataagttgc gacttaaaac tcaaaactta
      421 accaatgaaa attataatat taatgatgtg aaagttaaaa agattattga agatgaacgt
      481 caaagactag cacgagaact tcacgattct gttagtcagc aactttttgc ggcaagtatg
      541 atgctatctg ctatcaaaga aacgaagtta gaaccaccat tagaccaaca aattcctatt
      601 ttagagaaaa tggttcaaga ttcgcagtta gaaatgcgtg ctttgctgtt acatttaaga
      661 ccgcttggtt taaaagacaa atctttaggt gagggtatta aagatttagt tattgattta
      721 caaaaaaaag tgccaatgaa agttgtgcat gaaatacaag attttaaagt gcctaaaggt
      781 attgaagatc atttgttcag aattacacag gaagcaattt cgaatacatt gcgtcattca
      841 aacggtacaa aagtgacagt agaattgttt aataaagacg attatttatt gttgagaatt
      901 caagataatg gtaaaggttt taatgttgat gaaaaattag aacaaagtta tggacttaaa
      961 aatatgcgtg aaagagcttt ggaaattggt gcaacgttcc atattgtatc attgccagat
     1021 tcaggtacac gtatcgaggt gaaagcacct ttaaataagg aggattcgta tgacgattaa
     1081 agtattgttt gtggatgatc atgaaatggt acgtatagga atttcaagtt atctatcaac
     1141 gcaaagtgat attgaagtag ttggtgaagg cgcttctggt aaagaagcaa ttgccaaagc
     1201 ccatgagttg aagccagatt taattttaat ggatttactt atggatgaca tggatggtgt
     1261 agaagcgacg actcagatta aaaaagattt accgcaaatt aaagtattaa tgttaactag
     1321 ttttattgaa gataaagagg tatatcgtgc attagatgca ggtgtcgata gttacatttt
     1381 aaaaacaaca agtgcaaaag atatcgccga tgcagttcgt aaaacttcta gaggagaatc
     1441 tgtttttgaa ccggaagttt tagtgaaaat gcgtaaccgt atgaaaaagc gcgcagagtt
     1501 atatgaaatg cttacagaac gagaaatgga aatattatta ttgattgcga aaggttactc
     1561 aaatcaagaa attgctagtg catcgcatat tactattaaa acggttaaga cacatgtgag
     1621 taacatttta agtaagttag aagtgcaaga tagaacacaa gctgttatct atgcattcca
     1681 acataattta attcaatagt tcatatcgaa ttaagaaaag ttacttacgc caatcacaat
     1741 ataacatcaa atagacacca aaaaacagag gttttcgcaa taatcattag cgaaaacctc
     1801 tgttttgttg gtgtttagtc ttttttaagc ttatcaacga ttttgtcttt tatattttta
     1861 tcatcttctg gataggtatc actaggatgt tcatcaacat gatacgtacg ttgatgtgtt
     1921 gcattactat cttcatcata ttcgtatcta gtgttctcat tataatgatt tttattattg
     1981 tcatcatgtt ctaatgctgc ttcttcaggt gttttacctt taattacact acgctgatga
     2041 atgattgcat tgatttcagc accgacaatt ataataaaac ttgtgatata taaccatagt
     2101 aacaaaatga tgatacccgc gatactgcca tatgttttag aatagttacc aaaatttgaa
     2161 atataccaac caaaaccaaa tgaaccagct aaccaaataa ttgaagtaaa tactgcacct
     2221 ggtaatactg acttaagctt cgttttaacg ttaggtgcaa ccgaatataa cacgataaat
     2281 atgataaata taataatgat tggtaacaca attctaataa ggttaaaaat ccatttcact
     2341 tgttcgtcaa atccaagtgg accgaatagg aaatgactaa ttacagaacc aagcgttggt
     2401 aatgctagag caactacaaa cacaacgccc ataactacag tgaagacaac acttagtagt
     2461 tttaatacga ttccattacg gctatcttct acatcgtaag caacattgaa agaattcata
     2521 attgcagtca ttccatttga agctgaccaa attgctaaaa tcaaaccgat agataataag
     2581 ccaccactgg agttttgagt tatatcacca attacactct taattagagt agatgtttca
     2641 gcgggtgcat tgcttaacat attagtaatt tgactctgct taatattgaa aaatggtaat
     2701 aatgttaata ggaaaagcaa catagggaac atagcaagta cgaaatggta agtcatttgt
     2761 gctgctagtc ctgaggcatc atcttttcct atgcgataga tgagatatga taagaaatta
     2821 gagtctttct tatattttgc aggtttattt aatcttgata agaagaaaac ctgatcatct
     2881 tttttaggtt tcttagattg gaattgttga ggttcaatat atgtacgatc aacatcaatt
     2941 ttgggattac ttttattttt cttttttgac tcctcttgcg cttccttaac agaattaagg
     3001 tattttgaag aagagtgatc ctttttcgac atgtgcactc cttaaccttt ctctatgtaa
     3061 tgttttataa agtaactatt agtatttatc caattataaa aaaactaaac aatgatatgc
     3121 tttaaaaatg ataatattat agaaattaat tgatttaaat ctttcatagg ctattttgat
     3181 ttaaaattaa aacaaaggca atactgaaac aatgcagttt ggccaatcat tgtattatca
     3241 tttaataatg ataactatga aaggcgtaca ccaaattgtt aagtattgcc ttataaaaac
     3301 aaaatcatct tagcgttgat tttttctatt aacaaatgtt tctttcgcat cctttaatga
     3361 acgttctaat tcaggattat tacgacgaat ttcttcaaca acatctttcc agtataaaac
     3421 ttcgtcttta atcttgctga ctttagaagg cttacgagtg cggttaccat tttttgcatc
     3481 tttaactgat tgaactaaag cttgacgtgt agatttatca gctaaactaa ttgcaccacc
     3541 aattacggca ccaattaaaa taccaggaac aaatttattt tccataaaaa actacccctc
     3601 tttcaaattt gcatctttta cgatgtagtc tattaaatta tcacaagatg ataataccat
     3661 gtcgtataca ccttcaaaat tattcgtgta gtatggatct ggtacatcac tctcttccat
     3721 attactaaat tctaacagtt tgaacaattg tcccttaaga ttaggattga tagatttaat
     3781 attatcaacg ttactttgat ccatagccac aatgtaatca aaatcatctg tcgcttcgaa
     3841 taattcacta atcatgccat caaatggaat attgtgtttg ttgagaattt tttgtgtacc
     3901 ttcatgaggt ggctctccta aattccagct accagtacct cttgaatgta ctttaatatc
     3961 atgaatattt ctgtctttaa gtctttgtcg catgattgct tctgccattg gagaacgaca
     4021 tatattgcca agacagacaa atgctacatc taccattttg attcctccaa actatgtagt
     4081 tatatcccca ttttatagcg actttaaaca ataagaaagc agattatata aaattctatt
     4141 aaagtttatt aaattgtgat actttgataa cataactatt attagaggtg aacattgtgg
     4201 ctatgacaaa tgaagagaaa gtattagcta ttagagagaa gttaaatatt gttaatcaag
     4261 gattattaga tcctgaaaaa tataaaaatg caaatgaaga agaattaaca gatatatatg
     4321 attttgttca atcaagagaa agattgtcgc caagtgaagt gacagctatt gctgacgctt
     4381 taggacaatt gcgacacgaa taggagtggg aattttgact aattacaaag aaaagttaca
     4441 acaatacgct gaactattag ttaaggttgg tatgaatgtt caaccaaaac agccagtatt
     4501 tattagatca tcagtagaaa cattagaatt aacgcattta attgtagaag aagcatatca
     4561 ttgtggcgct tcagatgtac gtgttgtata tagcgatcca accttaaaac gcttaaaatt
     4621 tgaaaatgaa tctgtggaac attttgctaa tcatgagata aaatcatatg atgttgaagc
     4681 gagaatggat tacgttaaac gtggcgctgc taatttagca cttattagtg aagatcctga
     4741 tttaatggat ggtatcgata gccaaaaatt acaagcattt caacaacaaa acgcacgcgc
     4801 atttaaagga tatatggaaa gtgtacaaaa aaatcaattt ccatgggtcg tagctgcttt
     4861 tccatctaaa gcttgggcga agagagtgta tcctgaacta tctgtagaag aagcatatat
     4921 taagtttatt gacgaggtat tcgatattgt gcgaattgat ggtaacgatc cagttgaaaa
     4981 ttggcgtcaa cacatcgcta atttaagtgt ttacgctcaa aagttacaac aaaaaaatta
     5041 ccatgcgttg cattatgtat ctgaaggtac tgatttaaca gtaggtttag cgaaaaatca
     5101 tatctgggaa gatgctacaa gttatgttaa cggcaaggaa caagctttta ttgctaatat
     5161 accaactgaa gaagtattta cagcaccaga tcgaaataga gtagatggat atgttactaa
     5221 taaattgcct ttaagttata atggcacaat tatcgatcaa tttaaattga tgtttaaaga
     5281 tggagagatt attgattttt cagctggaaa aggtgaagcg gtattgaaag atttaatcaa
     5341 tactgatgaa ggttcaagaa gattaggtga agtagcatta gtacctgatg attcaccgat
     5401 atcaaatcgt aataccattt tttacaatac tttatttgat gaaaatgcag catgtcattt
     5461 agcgattgga tctgcttacg cctttaatat tcaaggtgga acggaaatga ctgttgagga
     5521 aaagattgca agcggattaa atgattcaaa tgtacatgtc gatttcatga ttggtagtag
     5581 tgatttgact atttatggca tattcgaaga tggttcaaaa gaactagtat ttgaaaatgg
     5641 aaattgggca tcaacatttt aataaatgtt attttgaggt gctgagtagg aaatgaaaca
     5701 tgtatttaaa ggtcaaatac gtgattaaag tatagattgg gagataaaat aatgacaaat
     5761 caggacagac caatgaaatc tatgtcagaa tcaaaatgtt ataaaaatag acaagttttc
     5821 cctcaagata cgaatcacca tcatacaatg tttggtggta cattgatggc taatattgat
     5881 gaaattgcag caatcacagc tatgaaacat gctggtgcgc aagtagttac cgcatctaca
     5941 gactcagtag atttcttaaa gccgattaaa acaggggaca tattacaata cgtagcgatg
     6001 gtttcatacg ctgggactag ttcaatggaa gtggtcgttc aaattagaat tgacgatgta
     6061 tttaataaca agcatgattt agctgcatta agttacttaa catttgttgc gttagatgat
     6121 gaaggcaaac ctaaacatgt acctggtgta tatccagaag atgatgttga aaaatggttt
     6181 tatgatacag caccacaacg tgttgaacga agaaaagcac gtcgcattga aagtaaacaa
     6241 acaattgaat atttagctca agtgcaacat attagagact aaaaatataa agacagattg
     6301 ttaaggatga attgaactgt tatgttcgaa tattccacct acaatctgtc tttttgatta
     6361 tgcacgttta tgcttttagt taaaatttac cccttttatt atagactatt agtcctatta
     6421 aatgcccttt attatttaag tagagtactg ccagcaatta attgtttttt attttataat
     6481 agcattaaaa tatgcggaga ctacttatcg ataaagtgct tctccgcata attaagagtt
     6541 tttaaaattg tataatttgt ggttgtttgt gcatatgacg ctcattatct ttaaaatcca
     6601 tattcggata gtacatattt ttaacaagaa catttggtcc taaacaacta aactcggaac
     6661 aatgacaatt taatgattta gcaagatcag aggataacca tttatcaaat acatctgtta
     6721 atttatcttt ttgtatattc gaaattgtac ctgtttcatc tccgaaatca gttacgatta
     6781 cattacctgt aaatacattg acatttaaac gactacggcc atccgggtca tttctagtcg
     6841 ttacattttt agcatttctt aaacgtgata gtaacttttg atcttcatca tcctttaagc
     6901 atggaaacac aggcaaagta ccaaataaca tccaaatatc ttcatctctg aaatccaata
     6961 tatcatgaat tgtctttttc atttccgcta gagttaacac atttaattga cttgcaaagt
     7021 cagctggata catagggtga atctcgtgtc tgctacattt catatcatga acgacttctt
     7081 gatgtatttt tcgtaaatgt ggtagcgtac tttgattgag cattgtttcc gcagatacaa
     7141 acattccttg ttctgataat gtacgtgcat tcgaaatcat ttgttcatat aattttaact
     7201 tagcttttaa cggtggttgc ttcttcattg cgccaaagcc aacatttgcg aattcatcag
     7261 ttgttcccca gttgtgtgag atatgcataa catcgatata ttcagcaata tctaaataac
     7321 gatcttgagg caatgttagg tttgaattca tttgtgtata tatacctcga tgatgtgcat
     7381 actttaatag aggtttaaca acatttctaa tagacttttt agaaaacatt ggttcgccac
     7441 ctgtaataga catcgttcgc agatttggaa tttcatcaag tctacgataa attaagtcca
     7501 tatctaaagg ctcggggtcg acagtttgta aagtataacc aactgcacag tggctacaac
     7561 gcatattaca aagatttgta gttgtaaatt cgatgttact taaagttaat tggccatgtt
     7621 ctttaacatc gttatatgct tcccatggat cattgtaaat gctaacaggt tgcttcaatt
     7681 tgacgctatt catcgtctta aacttccttc tttctatttg gttcatatcg gtatgatacc
     7741 acttcgtatt atcactcgta acaaattatc tcttaatttt agttaattga aaaggggatt
     7801 tttaaaagtc aaaataattt gaatgtcata aatatagcta agattatatc gctttaacgc
     7861 aatttctgtt aggataataa tgaaaaagaa aacaaaataa aaggagttaa ctttttatga
     7921 atgaacaaca aacaatcgaa cagataaaag cgcgtttaaa taagtttatt gaggatatcg
     7981 atcatgtaaa tcctgatgaa gtacgtgttg aagatataga tgaatggatt ggattgttag
     8041 atcagcttga agaaaaggtt aaattagtat ctaagtaaat gccacataag atgtgttagg
     8101 gttagatatc gcgacagtcg cttaatattt tttatatctt gtatgtttaa tcactgattt
     8161 acttgtctat agtaattata tcaatgatat atagaaaggt gatttgcaat gactaaaaaa
     8221 gtagcaatta ttctagcaaa cgaatttgaa gatatagaat attcaagccc taaagaggca
     8281 ttagagaatg caggctttaa tactgtagtg attggagata ctgcaaatag tgaagttgtt
     8341 ggtaaacacg gtgaaaaagt tactgtcgat gtaggcattg cagaagctaa accagaagat
     8401 tatgatgcat tattaattcc tggaggattt tcaccagatc atttacgtgg agatacagaa
     8461 ggtcgatatg gcacatttgc taaatacttt actaaaaatg atgtaccaac atttgccatt
     8521 tgtcatgggc cacaaatact aatagataca gacgatttaa aaggtcgtac gttaacagca
     8581 gtattaaatg tacgcaaaga tttatcaaat gcaggcgcac atgtagttga tgagtcagta
     8641 gttgtagaca acaatattgt aacaagtcga gtaccagacg atttagatga ttttaatcga
     8701 gaaatcgtta aacaattaca atagaagtat tgtggttatc gattgttaaa atttatatat
     8761 tttcgtttga aatgaaatat ggattttagt tttaataaat gaataacaaa taaaaggtcg
     8821 tctctattgg catttaatag ggatggtctt ttatttttat aaaatataac aatatttgac
     8881 atgattaata tttaaaattg caatgattta tctataattt tttcaaaaaa caaaaaagta
     8941 agtatgatgg cgtatacgat tgtaagccct tggactgatt ttcccaaaaa gtgtagccta
     9001 tttgtgatag tactggtaaa ctcaaggtat atactaagtg agttttaaaa gaaggagcaa
     9061 acgcatgaaa agaagcgata ggtactcaaa ctcaaatgaa cattttgagc atatgaaaca
     9121 cgaacctcac tataatacgt attatcaacc agttggcaaa ccgccgaaaa agaaaaaaag
     9181 taaacgaata ctattaaaaa tattattaac cattctaatc attatcgcat tgtttattgg
     9241 tatcatgtat tttttatcta cacgcgataa tgtggatgaa ctaagaaaaa ttgaaaataa
     9301 aagtagtttt gtgtcagctg ataacatgcc agagtatgtt aaaggtgcct ttatttcaat
     9361 ggaagatgaa cgattctaca atcatcatgg attcgatttg aaaggtacaa ctagagcttt
     9421 attttcaacg attagcgaca gagatgtgca aggtggtagt accattacac aacaagttgt
     9481 caaaaattat ttttatgata atgatcgttc atttactaga aaagtaaaag aattatttgt
     9541 agctcatcga gttgaaaaac aatataataa gaacgaaatt ttaagctttt atttaaataa
     9601 tatttacttt ggggataatc aatatacgct tgagggcgca gcaaaccatt actttggaac
     9661 aaccgtgaat aaaaatagta caacaatgtc tcacataaca gttttacaaa gcgctatttt
     9721 agctagtaaa gtcaatgcac ctagcgtata taatatcaat aatatgtcag agaattttac
     9781 gcaacgtgta agcacgaact tagaaaaaat gaagcaacaa aattatatca atgaaacaca
     9841 atatcaacag gctatgtcac aattaaatcg ttaaatactt gcattcatgt gtaagatgct
     9901 tggaactata atgtagaagg tttaattgaa atgtcataat gataaagttg aattgtttct
     9961 gctttataaa ttttacagat ttgcttgtaa tcacactttc attttaaaag atgatagtgt
    10021 gattttttgt tagcatcgaa aaatatttaa agttaaacat ttgctatagg aagtggctta
    10081 ttgaataata aaagagtatt gtattaaaac ttaagaaagt aacggggtga tccagtgccg
    10141 aagattacta aaatagaagt tcaaaagaaa aacaaagaac ggtttaatct ttttttagac
    10201 gaacaatttg aaatgggtat agatattgat acattagtca aatttaattt aaaaaaaggg
    10261 caacaacttg aagctgctga catggcagag attcaaaagt atgatcatta tcgcataggt
    10321 ttaaataaag caatccaata tttatcatat aaaaagagaa ctgaaaaaga agttatacaa
    10381 tatttacaaa aagaagagat atcagagcaa gcgatttctg aagtgattga atattgttat
    10441 cgcgaaaagt taatcgacca tcaagattat gcggaaagtt taaaaaatac aatgattcgc
    10501 acgacagata aaggacctaa aatttatcaa caaaaactat atcaacttgg tatagaacca
    10561 aatatcattg aaatatttac agaactttat agagaacaac aggaacttga tgatattatc
    10621 caaattgctg aaaaaatatc taaaacaaaa aaagggccac aaaataaagt taaagaaaaa
    10681 gtaatgcaat ctttaattca aaaagggttt gaaatggaaa ccattcatgc tgtattgaat
    10741 gaaatggatt ttac
//
LOCUS       DANJFZ010000090        10591 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.090, whole genome shotgun sequence.
ACCESSION   DANJFZ010000090 DANJFZ010000000
VERSION     DANJFZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.090"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(241..329)
                     /locus_tag="PWN45_002011"
     tRNA            complement(241..329)
                     /locus_tag="PWN45_002011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Ser,seq:gga)
     gene            complement(384..455)
                     /locus_tag="PWN45_002012"
     tRNA            complement(384..455)
                     /locus_tag="PWN45_002012"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Glu,seq:ttc)
     gene            complement(457..531)
                     /locus_tag="PWN45_002013"
     tRNA            complement(457..531)
                     /locus_tag="PWN45_002013"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Asn,seq:gtt)
     gene            complement(546..619)
                     /locus_tag="PWN45_002014"
     tRNA            complement(546..619)
                     /locus_tag="PWN45_002014"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(585..587),aa:Gly,seq:tcc)
     gene            complement(635..710)
                     /locus_tag="PWN45_002015"
     tRNA            complement(635..710)
                     /locus_tag="PWN45_002015"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:His,seq:gtg)
     gene            complement(724..796)
                     /locus_tag="PWN45_002016"
     tRNA            complement(724..796)
                     /locus_tag="PWN45_002016"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Phe,seq:gaa)
     gene            complement(813..888)
                     /locus_tag="PWN45_002017"
     tRNA            complement(813..888)
                     /locus_tag="PWN45_002017"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Asp,seq:gtc)
     gene            complement(910..983)
                     /locus_tag="PWN45_002018"
     tRNA            complement(910..983)
                     /locus_tag="PWN45_002018"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(947..949),aa:Met,seq:cat)
     gene            complement(1455..2009)
                     /locus_tag="PWN45_002019"
     CDS             complement(1455..2009)
                     /locus_tag="PWN45_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL0623770.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2332..3732)
                     /locus_tag="PWN45_002020"
     CDS             complement(2332..3732)
                     /locus_tag="PWN45_002020"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDL0623771.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3756..4679)
                     /gene="hemH"
                     /locus_tag="PWN45_002021"
     CDS             complement(3756..4679)
                     /gene="hemH"
                     /locus_tag="PWN45_002021"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL0623772.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4737..5774)
                     /gene="hemE"
                     /locus_tag="PWN45_002022"
     CDS             complement(4737..5774)
                     /gene="hemE"
                     /locus_tag="PWN45_002022"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL0623773.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5764..5874)
                     /locus_tag="PWN45_002023"
     CDS             complement(5764..5874)
                     /locus_tag="PWN45_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623774.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            6037..6540
                     /gene="traP"
                     /locus_tag="PWN45_002024"
     CDS             6037..6540
                     /gene="traP"
                     /locus_tag="PWN45_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDL0623775.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(6664..7887)
                     /locus_tag="PWN45_002025"
     CDS             complement(6664..7887)
                     /locus_tag="PWN45_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623776.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(7880..8620)
                     /locus_tag="PWN45_002026"
     CDS             complement(7880..8620)
                     /locus_tag="PWN45_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623777.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            8754..9176
                     /locus_tag="PWN45_002027"
     CDS             8754..9176
                     /locus_tag="PWN45_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL0623778.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            9318..9683
                     /locus_tag="PWN45_002028"
     CDS             9318..9683
                     /locus_tag="PWN45_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0623779.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            10416..>10591
                     /locus_tag="PWN45_002029"
     CDS             10416..>10591
                     /locus_tag="PWN45_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDL0623780.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLV"
BASE COUNT         3464 a         1973 c         1555 g         3599 t
ORIGIN      
        1 actaaattta tagaaaaaaa tctgaatgat ttgttttgtg actaaacttc aaaatcttgt
       61 gactaaaatt aggattattc atcaaaaatt agtcacattg tggctaaaaa attttaaatg
      121 gatataaata aataagaagt gtaaaaatga ggataatagc tcaaatgcag atatatcaag
      181 ggattgagtc caaaataaaa atagcgctta aaatgggtaa aaccaattta agcgccttta
      241 cggaaggtaa gggattcgaa cccttgagac gcttgtgacg cctacacact ttccaggcgt
      301 gctccttcgg ccagctcgga caaccttcct caacaataaa tatgtaataa aaaaacagaa
      361 gcgatatttc acttctgctt gtatgactcc tacgggactc gaacccgtgt taccgccgtg
      421 aaagggcggt gtcttaaccg cttgaccaag gagccatggc tccacaggta ggactcgaac
      481 ctacgaccga tcggttaaca gccgatagct ctaccactga gctactgtgg aataatagaa
      541 aataatggag cgggtgatgg gaatcgaacc cacaacatca gcttggaagg ctgaggtttt
      601 gccattaaac tacacccgct aatgtaaacg attatggggc ggctgaaggg gatcgaaccc
      661 tcgaatgtcg gaaccacaat ccgatgtgtt aaccacttca ccacaaccgc catgaattaa
      721 gaatggttca ggacagagtc gaactgccga cacatggagc ttcaatccat tgctctacca
      781 actgagctac tgaaccgtaa tggtaataat aatggcggtc tcgacgggaa tcgaacccgc
      841 gatctcctgc gtgacaggca gccgtgttaa ccgctacact acgagaccta ttaaattaaa
      901 aactatgtat tgcgggaggc ggatttgaac caccgacctt cgggttatga gcccgacgag
      961 ctaccgaact gctctatccc gcgctaatat tattttgaat tacctaatta atataccata
     1021 atcaaaaacc taaagtcaag aactttttga atttaattta aatgttatct cttaaataat
     1081 tacttaaata tcgtagcaac atgttctctg ttgaacacaa atattagtat attcattttt
     1141 gtagtgtacg tcaacgacat tttcaaagtt ttttgtgtaa aaaaacgctt cttattccct
     1201 tttatcatat aagtgtctaa tagttgtcat aaatagtgtt aaagcattta aaaaggtata
     1261 ggagttataa agtttacaac gcctatacct tctgaaaaag aaattataaa agcttgttac
     1321 accgcatatt ctttcagtca gcgactacca atataacatt gtagccctaa gacattgctt
     1381 gacgcctcaa ttacaacaat ttttcaaaat cagcagctac ctactgacac aacataacac
     1441 aacccataca ctaactatcg tgtcatgtaa tcttgcatcc gatcttgcaa cgctgtaaat
     1501 gtttcgaagc catcctcttc taagaagtgc cctccatctt ccacgattcg caagttcccc
     1561 tctaatgcat tcattaaacg ctgggtctct ttatatgaaa cgtatttgtc atttttagaa
     1621 ctcaatccgt aaaaattgtc aactttcttt ttaatattat cgtaatcaat ggttacatta
     1681 cttaaatcaa tatctaaatc tatattttct gcatcttctt taaagcccgc tatactgaaa
     1741 aagccttcaa tcggctgatc aatcatttca atatatttta aagctgtgat tgaacctaaa
     1801 ccatgtgtta caaaatatgt atcctttttg cgtacattaa tttgtttcgt catagcttca
     1861 atccactgat ccactgtctt cgcttcaggg gattcaaaat taaataatgt tacgtcatat
     1921 ccttctaaag ttaagttatg ctccaaccac tgataccaat gatttctact atttccatgc
     1981 atagaatgta caataattac atctgtcatc tcattctctc ctttcaactt actacttctt
     2041 ttctattttt aaaaaaatga ctgattacct ataattgtaa aataaaaaca ccttaattag
     2101 aaatgttata tcgcaaagtg acatttctaa ttaaagtgta ttgtcatcat ttcaatatca
     2161 ttcaaaaaca gctaaacctt tgtctctgct tcaatttcac aaaaataatt cccgctgaaa
     2221 gtatctatat ttacacatta cttccaccat tatataactt aaaaatgact atatttcatc
     2281 aaacattatc taaaggcgtc gcacctacac caacaccatc caacaattaa cttacaactc
     2341 tgcgattact tcttcagcag caactttacc ttgcgtaata caatcaggta gtccaaccgc
     2401 ttcaaaagat gcaccagtta ctctaagtcg tggatatgtt tgtttaatat gtgcttgaat
     2461 ctgtctaatt tgttgaatat gaccgacatg gtactgtggc atacttttcg gcaaacgatt
     2521 gacaattgta aattcaggat cacctttaaa tgtcatcatt tgacttaaat ctctacgtac
     2581 aatcgatact aattcattat ctgtatgatc atcaaccaca gtatcacctg gtttacctac
     2641 atacgcacga atcaaaacct taccttccgg tgtagtaaat ggccattttt tcgatgtcca
     2701 agtacatgcg gtaatgtctg tatcactcgt tctcgcaatt acgaagccag taccatcatg
     2761 ggtattttca atgtcttttt catcaaatgc caatacaaca gttgcaacag tcgtactatc
     2821 catcgtttta aagtaatcaa atgctggatc ttgtccgaac caatttaaaa acacttgatg
     2881 tggtgtcgtt actaatacgc catcatacac ttcttctagt tgatcattgt aaacaatttt
     2941 atattgtttt tgagatgtaa ttatatcatc cactgacgta ttgtagcgta ttgtcacacc
     3001 tttattttta acatcttgtt ctaatgcttc aataaatgag cttaaaccat gcttaaattg
     3061 tttgaattgt cctttcggtg cgccaggata taattgtctt tgtttcagac gcttattttt
     3121 ctcatccttc atacctttta tcagacttcc gaatgcctct tctttttctt taaaattagg
     3181 aaacgtactc atcaaactta atttatcaat atcggtacca taaataccac ccattaaagg
     3241 ctctattaaa ttctcaagta cctcattacc taatcttgct ctgaaaaatg caccaacaga
     3301 aatgtcacca tcctgcattt gagtaggttt ttttaataaa tcaaaccctg ctcttaattt
     3361 accaagtggc gatattaatt ttgtagtaac aaatggttta atatctgttg gaatacccat
     3421 aattgaacca cctggaatcg gatataattt atttttcgca aaaatatatg attgtccagt
     3481 cgtatttgta acaatatctt gttctaatcc aatatctttc gctaattctg tcataatcgt
     3541 ttttctacct aaataagatt caggccctag ttcaatcata taaccatctt tacgatacga
     3601 ttgaatcttt ccccccggac gattcgatgc ttcaaagatg gttacatcaa tattaggatc
     3661 ttgctgtttt aaaaaatatg cacttgataa acctgttatc cccgctccta taatagccac
     3721 tgatttagtc acaacgtttc acgcttcttt cgtcattaaa atatagactt gatttcatca
     3781 acaattgcac cgataaataa tggatgtgta ttcggcattt ttggacgata ataattcgca
     3841 ccaatatcat cgcaaactac tttacattca taatcattgt cataaagcac ctctaaatgc
     3901 tcacatacaa aacctactgg cgtatatata aagtttttat actgatgttt ttcatataaa
     3961 tcacgtgtta aatcttgtac atctggccct aaccaaggtg tacctgtatt accttcagat
     4021 tgccaaccaa tcgcgatatg ttcaatatta gattgttctt taattaaaag cgcagtatgt
     4081 tctagttctt gtggatatgg atcattattc ttttcgatta aaccttttgg caaactatgt
     4141 gccgaaacaa ctaataccgt gtctttatgt tcctcttccg gtatttgagc taatgtttcg
     4201 ttgactttat tcgtccaata ttcaataaat ttaggttgtt cataataatg tttcacatgt
     4261 gtaagttgaa taccatattt tgcagcttct tcatcagcac gtttgtcata tgatcctact
     4321 gaaaatgaag aataatgtgg tgctagtact accgtgattg cttcagtaat gccatcattg
     4381 tgcatttgtt caaccgcatc ttcgataaat ggtgaaatgt gttttaatcc taagtatagt
     4441 ttaaattcaa catctgcata tgctttattt aatgctgaaa ctagcgcatc agcctggtca
     4501 tctgttgtac ctgctaatgg tgataaacca cctataaatt catatctatc tttcaaatct
     4561 tgaagttctt cttcagatgg acgtttacca tgtctaatat ctgtataata tggctctatg
     4621 tcactttctt tataaggtgt gccataagcc ataactaata atcccatttt tttagtcatt
     4681 gataatacct tcctttaaat gaattatctt tcatgtgctt caatgtaaaa ctatgattat
     4741 ctttgtgtat atgtgtgtac gaattcgctt actttacgta acgtctctgg ttgcacttct
     4801 gggaatacac cgtgtcctaa attaaagatg tgtttaccgt tctccatacc ttgatctaat
     4861 attggtttca atctctcttc aatgacattc catggtgcta ataaaattga tggatctaaa
     4921 ttcccttgta atgttttagt aacgcctaat tgttgagcct gattaataga cgttctccaa
     4981 tctaggccta atacatcaat cggtaaatca ttccattcat tgattaaatg actggcacct
     5041 acaccgaata aaattaccgg cacatcatgt ttttctttaa cctcactgat taatcgaatc
     5101 atatgtggtt taatgtaacg tctgtaatcc tcgacattta atgcacctac ccatgaatcg
     5161 aaaatttgaa tcaattcggc acctgcttcg acttgagctg ttacatattt aacagataca
     5221 tcaactaaat gattcattaa agcaaaccat gttgcttcat ctctatacat catcgctttt
     5281 gtaaaattgt aatttttcga tggtccgcct tcaatcatat atgacgctaa tgtaaatggt
     5341 gccccagtaa atcctattag cggcacattt aacttttctt ctgttaaaag tttaattgta
     5401 tctaatacat atggtacatc tcgttcgggg tctatttgag aaagtttctc aacatcttga
     5461 attgttttga ttggattatg aatcactgga ccaatacccg atttaatttc tacatcgaca
     5521 ccaattggct ttaatggtgt cataatatct ttgtataaaa ttgctgcatc tgtatgataa
     5581 ttatcaactg gtaaatgtgt tacataagcg cacaactccg gctgatgtgt aatatcgaat
     5641 agtgaatatt tttctttcaa ttttcgatat tctggttgcg aacggccagc ttgtcgcata
     5701 aaccaaacag gtgtatgtga tgtttcttca cctttgatca tttttaaaat tgtattgttt
     5761 ttattatgca ccataaaggc ctcctaaatt aaaatcattc ttatctatat tatcatatcg
     5821 ctcattcgtt cgtattttca ataaataaat gtcataaaac tgacatttaa tcatagaact
     5881 atttattgta aatttaaatt ctaaagtcca ttattttgta tcattacttc taaatatctc
     5941 gcaagattca ttatagtaat tttaatcaat tattaatagt ggtaatgact agtttatcat
     6001 cgtataataa ataaaaacat aagggggacc tttcatatga agaaactata tacatcttat
     6061 ggcacttatg gatttttaca tcagataaaa atcaataacc cgacccatca actattccaa
     6121 ttttcagcat cagatacttc agttattttt gaagaaactg atggtgagac tgttttaaaa
     6181 tcaccttcaa tatatgaagt tattaaagaa attggtgaat tcagtgaaca tcatttctat
     6241 tgtgcaatct tcattccatc aacagaagat catgcatatc aacttgaaaa gaaactgatt
     6301 agtgtagacg ataatttcag aaactttggt ggctttaaaa gctatcgttt gttaagacct
     6361 gctaaaggta caacatacaa aatttatttc ggatttgctg atcgacatgc atacgaagac
     6421 tttaagcaat ctgatgcctt taatgaccat ttttcaaaag acgcattaag tcattacttt
     6481 ggttcaagcg gacaacattc aagttatttt gaaagatatc tatacccaat aaaagaatag
     6541 attcgaagaa aacttccatt ggttatgctg ttaacgtata gatgctaatg taatctgaac
     6601 gccgtatcat ttggcgttca acattacatg cacctttaca acatacatgc caatggaagt
     6661 tttttagtct cgtaataatg tttcctgata tttcaacttt ttaataatac ttctaatcgt
     6721 taataaacct acaatataga aaatgagtac aacatagaat aacgtcatat gttttataat
     6781 aaatgtcact gcaaacacag tacaaataac aaacattaat ctatataaaa actgttcata
     6841 ccctttgatt accttctctt caggtacagg ccacacttga ggccataagc catatgcttg
     6901 ctgtgaataa aattgtgcca tttgtaacaa tataatatat acaaataaac acccaataat
     6961 tgctgtcact aatggatatg ataaccaaac cattaataaa actgcaataa ttactaacct
     7021 aaagataata ttaaatgcgt ctctccctct tataaagctt ctaataaata agaataaata
     7081 catcgcatta gagttaaatt tactaccctt tggaactggt aaaagtatat ctagataact
     7141 tcttctgact gcagattctt tcaaatgttt tacatcggtg aacatattaa caaatttata
     7201 ataattcata tgatgtcgat gttcgattgc aatcattttc tcccaaggat acaaaaagcc
     7261 tggtttatat tttttaacta aaaattctat taacacaggc aaagcaacca tcacaaatgc
     7321 gatgtaccat tttggagcta atagtaagta atatgttaga gcaaaggtga tgaatgatat
     7381 taaattaact tgccatgttt taagtcccga ttgataccat tgccatctta agcgtaaacc
     7441 aacatatgga aaaattaatg cactgactcc aaaacaaata taaaatgcca cattatgttg
     7501 attaatattg taaaacaacg ggaacattac aataacaata atgagttgga ttaatatgcg
     7561 cgcaaagtaa ctatataaaa tcgcatgacg cataaattga gacatgtgtt tttcaaatgg
     7621 taataaaaag attttatccg cttcttttaa cagtggtcgc attggaaaaa tagatgtcaa
     7681 cgcaacaatc actgctgcta ttaatgaaaa attgatattc gttggaatat gttttaacca
     7741 ttcaccatat ccaaaaataa atgcacccag caaaataagt aaaaagacca tgaaatgacc
     7801 attaaatata aacttattat aataattttt ctctttacga agggcatgta atcttttatt
     7861 aaataatgtg gtagcttggt tacgcatgta catctccacc ttgcgtcaca tgaatatata
     7921 tatcgtctaa tgtttgatta tgtaagccag tttgttgtct caatgcttct aaatctccaa
     7981 atgcaacgac ttcaccttcg tctagtatga taaaacgatc acagtaacgt tcagctgttg
     8041 ctaaaatatg tgtactcatt agaacggttc taccttcgtt tttcttttca accattaaat
     8101 ctaacatgga ttgaattcct aatggatcta ggccaaggaa tggttcgtct ataatataca
     8161 attcgggatt aacgataaac gcacaaataa tcatgacttt ttgtttcatc cccttagaaa
     8221 aatgactcgg aaaaactttc aactcatttt ctaaacggaa tgtctttaat aatggcattg
     8281 ctcgattcat cgtttcatca cgatcaatat catatgccat tgctgtcatc tcaatgtgtt
     8341 cctctaatgt gagttcttca taaataaccg gtgattccgg aatataagat aactttcttc
     8401 tataagcctc tatgtcatca ttaatgttga tatctgaaat tgatagagat ccttccatag
     8461 gtgtaagcaa tcctagcata tgtttaatcg ttgtactctt accagcgcca ttaaggccaa
     8521 taagtccaac aatttcgcct ttgtttaatt caaaatttat atctttaatt acagggcgtt
     8581 ttccatatcc acctgtaagc tgttctactt taactgtcat aaggcacctc catgacttat
     8641 attgtaccaa aaattataaa atgctcatat taaatacaca tgtcctaata tcgaattttt
     8701 agcgacaatg ttataatgaa tggtaatact agttgaaaag gagtgtagtc atcatgtcag
     8761 aaacaatttt cggcaaaatt ttaactggag aaattcctag ctttaaagta tatgaagacg
     8821 attatgtcta tgccttttta gatatatcac aagttactaa aggacatacg ttattaattc
     8881 ctaaaaaagc ttctgctaat atctttgaaa ctgatgaaga aacaatgaaa catatcggtg
     8941 cagcattacc taaagtagca aatgctatta agcgtgcatt taatcctgat ggtttaaaca
     9001 ttattcaaaa taatggtgag tttgcagatc aatctgtatt tcatattcat ttccacttaa
     9061 ttcctcgata cgaaaatgat attgatggat ttggttataa gtgggaaaca catgaagaca
     9121 ttttagataa cgatgcaaaa caacaaattg ctgaacaaat tcaagcacaa ttttaaatgt
     9181 atgcttaatc taagctcgaa cgggtataat atgattaata ttataacaat tgcgtttgaa
     9241 gtgataacat caaggttagc aattttaaac aaaatgagtt atcaagataa cagatgttaa
     9301 aagtgaggag aatataaatg aaagcatcac gcattctatt cggtatcggt gttggcgtag
     9361 cagctggttt tgtagttgca cttcaaggac gtgacgacaa aagtgtcaag aacaacacga
     9421 tcgatcgtac tgcccctact ggttcaaaat cagaactaca acgtgaattt gaaacgatta
     9481 aacaaagttt taatgacatt ttaaactatg gtgttcaaat taaaaacgaa agtgcggaat
     9541 ttggtagttc aattggtggt gaaattaagt cattacttgg aaacttcaaa tctgacatta
     9601 atcctaatat tgaacgttta cagtcacaca tcgaaaattt acaaaatcgt ggcgaggata
     9661 ttggaaacga aatttctaag tagcaggtta cgttctcgat cacaactatt tttattagta
     9721 acagcatatt tattttttaa aattaaatgc caaataaacg agatgacatt agaaattaga
     9781 tatttcttgt catctctttt ttaaaactca aatgaactta tgtttacaaa ttataggaag
     9841 acattgtttg tagtgatttt cgcttaaatc atatttatga attgattgaa aacattgctt
     9901 aggattcatt gtgttatcct tgcactttga ttacgcttta cttaaatcat tatcgacaaa
     9961 caacatactt atattttcat tgagccgaac cttatataca cattacatat accttacttg
    10021 cacaaattat taatctggtg tttattataa ttacatatca ctatattttt agcatttgta
    10081 taacttagtt ggtcaaaaga tgcttttgca tatgccctgc catatccatt ttttatatta
    10141 ttcaagtttt caaatactca tgtcacacgc gcctttttca taagtgatgc tttattagca
    10201 agaatatgtg ttcgcagaaa tttgttctgc attctacttc tacgctagtc aatcagacaa
    10261 ttttaccaat ccccactttc gcgtttcaaa tcaaacaata cgtcgctcct ttcttcttat
    10321 ataacaattc ttctaacatg atatgttact attgaattac tgaacctgag ttagttataa
    10381 tctaacttat attgaaaaga gatgaggcgt aagatatgtt tttatgtaaa agacaaattg
    10441 atatcaatgc acgatttggt ttgcctagaa ttgcatttat gagtgcagtt gcaaccatca
    10501 ttatgttttt agttagttat gaagtaatgt attttttatc taatacgcca ttatcagata
    10561 gacattttct catcttttta ttacttgtat t
//
LOCUS       DANJFZ010000091        10534 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.091, whole genome shotgun sequence.
ACCESSION   DANJFZ010000091 DANJFZ010000000
VERSION     DANJFZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.091"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(319..1692)
                     /locus_tag="PWN45_002030"
     CDS             complement(319..1692)
                     /locus_tag="PWN45_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDL0623781.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(2364..2594)
                     /locus_tag="PWN45_002031"
     CDS             complement(2364..2594)
                     /locus_tag="PWN45_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623782.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(2625..3563)
                     /locus_tag="PWN45_002032"
     CDS             complement(2625..3563)
                     /locus_tag="PWN45_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDL0623783.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(3998..4663)
                     /locus_tag="PWN45_002033"
     CDS             complement(3998..4663)
                     /locus_tag="PWN45_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0623784.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            4815..5135
                     /gene="czrA"
                     /locus_tag="PWN45_002034"
     CDS             4815..5135
                     /gene="czrA"
                     /locus_tag="PWN45_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDL0623785.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            5137..6117
                     /gene="czrB"
                     /locus_tag="PWN45_002035"
     CDS             5137..6117
                     /gene="czrB"
                     /locus_tag="PWN45_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDL0623786.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            6383..7474
                     /locus_tag="PWN45_002036"
     CDS             6383..7474
                     /locus_tag="PWN45_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0623787.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(7974..8078)
                     /locus_tag="PWN45_002037"
     CDS             complement(7974..8078)
                     /locus_tag="PWN45_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623788.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(8239..8722)
                     /locus_tag="PWN45_002038"
                     /pseudo
     CDS             complement(8239..8722)
                     /locus_tag="PWN45_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(8765..9901)
                     /locus_tag="PWN45_002039"
     CDS             complement(8765..9901)
                     /locus_tag="PWN45_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HDL0623789.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTD
                     IDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            10190..10282
                     /locus_tag="PWN45_002040"
     CDS             10190..10282
                     /locus_tag="PWN45_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623790.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
BASE COUNT         3464 a         1857 c         1348 g         3865 t
ORIGIN      
        1 gtatttttta tttactttaa atttctcaat ctacagtgta atcttatttc ttattattaa
       61 cttagcgttt ggtcattcgt cttattcaac ataccactgg aaaactcata taaagtttta
      121 attctgtaac ataaattctt ttatattcat atactgtctt tattatttat taaaataaaa
      181 acgagtaatc tatcataagc tcttaattag ctcttatggt tgattacccg tcattttttc
      241 tttatgcggt tattcaaata tgcaattatg caatttttaa tcctatcctt acttcttttc
      301 aaaaactacc atatatcatt agtcatgttt gactttgact atacgtgtgt attttaaaaa
      361 ctgctctgaa tatttctcct taatttcctc taaactgtca tatgtaatac ctacaatatg
      421 ccaattagga ttgaaataat agaaagaatc tttttgtcgt gaccatttaa tcatattacc
      481 gtctttatta taacgtggta atgttacttc atagccttct aacacattaa tatacgtttg
      541 gaaaatgtct gtatctatac gattaaaatt atcaataact aaataacttt tatccttttt
      601 aggcattaat tgagtaataa acccttcacg atagtataat gcccctgttt catttggtaa
      661 atacttgcca taaagcatat cttctgaaaa atcaggatga cctgttgtta taacaggatt
      721 tgcattcgct ttttgcaata agtcattagc cgcttttaag ccttcttctt ttcgatctac
      781 aacaagtaaa ataaatggct ttaacgcttc ttctttttca ctttcaagca tatctggttt
      841 ttgaaccatt tcaaatggag atttcaatcc attattatcg ctcatttcaa taattgcatc
      901 atactgtgct tgtgacatac ttgcaatagc ctgttttgca ttttcttgaa ggaaatataa
      961 gtttttcaat ttaggatgct tatttaatgt acttaatgta atcggtgtaa tgtctttctc
     1021 ataagacact tcaatcactg tactatttgt tctaccagga attggtggtt tttcatgaat
     1081 atgctttgat acttctccaa ttccaacgac agattgattt ttcgttcgat tataaaaaat
     1141 aatattgtcg ccttcttcta actgagtata aaaatgataa ccattacgtt taataccgtt
     1201 gtacgtgtgc gtataaatcg tatattggtt tccaggttca aattcttcag tttcagctaa
     1261 aaagaaataa cgcggtatct taatttcgcc tttaccaaga ccacttatta aatcaaactc
     1321 ttctgcagtg atttgattga acaatgtctc tttcatatta cttatacgaa attccaaagc
     1381 ttcactacgc tttaaataat ctgctgttaa tggtttcaat tgttcattaa aacgaaactg
     1441 tacacgtatt ttattttgtg cacctgtttc aacactaata atttcaccac atccaagtag
     1501 tccagtatcc gtctgaactt gataaaagat gacttgatct cctactttag cctttttaaa
     1561 cgctctaaat ccttgagatg ggttaaaatg tgcgcctgat tcaaataaag ctgtttgtcc
     1621 tactaacggt tcattatgat tccaacggtt atatccacaa ttcaaccaaa aataattcgt
     1681 ttctgctgtc atcttaatac tccttaacct gaataaattt tagaaacact atgaattaca
     1741 ttcttttagt gtttcttatg cagttggacg cgtatgcgaa caactgtata ccctttgttc
     1801 actgcgattt taatcgcatt tcctataaca ttgtagcgcc caggacatta atttacgtcc
     1861 cagaccctta tcgttttcac ttctaagtaa gtcgaactat tttgctttac aacaagtgcg
     1921 actctaaata cagttggaca cacatacgag caactgtata ccttttaatc agttttctat
     1981 attttattta ttatatctgt cttaatgata aaaattgtta caaacagttt aacatattta
     2041 gctacctatt ttgcatataa aaaacatgct tgcatacact atgcaataaa aattacaaat
     2101 ttatatatga taccactatg cttgcttatc tctatagcgc cattgataca catttttaaa
     2161 tatctatact gccgttagaa ttttatcatg tcttaatttt cattaaatat taattacttc
     2221 attttatata aaccaacaaa aaccccctca ctactatgca agtgagaggt tatgttgatg
     2281 tgctttattt tcatcaatgt cattaacaca atcatatttc ttctgttaat gcactgtatt
     2341 atattcaaac gcgctatcga caattaattt gaagctacaa aggtaccgca taatggcagc
     2401 acattaatta acaatgcgct aaactttaat gaggcgctga tttgcttgtc tttaaaagtc
     2461 aacatagcac tgataattcc taaaatagct actatacgtg gaattgtcat gctcggatac
     2521 tgcggtatat gtaagaaagc accaagacct gctatcacac cacatactaa cgcaatatat
     2581 agaaattgct tcaaatcatt cactcctaaa ttgttattac actattacac atagctaatc
     2641 atcaatgtga aatcaccttc aaagacacta tccaaatctt cagaagtcaa aataaagttt
     2701 gtaccagtag tcagtttgaa aatttcacca tcgacaatca tttgcccttc gccttccaac
     2761 actgtaacta aacagaactc tctaggcttc atataattta acgtgccaga aatttcccat
     2821 ttaaccaatg taaagaaatc attcgataca atgtgtgtac acttatggtt ttcaataatt
     2881 tcgctttcag gcaaaatatt aggtaatggt gcattgtact gaataacgtc taaagctttt
     2941 tcaatattta acggtctatc attatattga ttatcttgac gattgaaatc ataaagtcta
     3001 tatgtaatgt ctgacgattg catcgtttcg tatgctaata ttcctgaaga aatagtatgt
     3061 actgttcctg ctggaataaa atagaattct ccaggtttta ctttaatata tctaagtatc
     3121 gactctatcg ttccgtgttg aacatgattc gcaacttctt ctctagactc tgctaatgtc
     3181 cctataacta tttctgcatc ttcttctgca tctataatat accaacattc agatttgcca
     3241 tattgcccgt tttcatgctc ataagcataa gaattatcag ggtgcacatg aatagaaagt
     3301 gattctcttg catccactat tttagttaga agcggaaaat ctttgcttgg gaaatcacca
     3361 aacaattcac gatgttctga ccaaatacgg tctaatgttt gaccttgata tggtccatta
     3421 ataatctcgc tcgtaccatt tggatgtgct gacacacacc aacattcccc agttgtatca
     3481 ttgtctaatt gatatccaaa ctcacttaga cgttgaccgc cccataattt tgtttttaaa
     3541 attggttgta aaaataatgg cattgttgca cctccattgt gattaagtaa gcaatagaac
     3601 tctgatgttg ttgttccatt atattttgat tttgttctca tttacatcgt attattaact
     3661 tccacatttc aaattaacta ttagtgattg taccatattt actaacattg cagtactgcc
     3721 aattaaagcg cttcacttaa atttacagta ctttaacatt ttcaaaaatt tatagcatag
     3781 agattatatc tctcttacat ttgtacatat ttccctttaa atttactcgc ccattatacc
     3841 aattaataaa caactttaat agttgtgcca tacattgttc aaattctttg taaaacgcat
     3901 agacaatacg tacttattca tacttataat tcatcatttt caaaaaataa cgagttacga
     3961 aaaagtaacc cgcttcaaat catatttact atccttatta atccgtttca ttttcaaatt
     4021 gagttaaagc atctttaatg tcctgatcac cactaataat ttgaaactct tggtgattaa
     4081 aatgattgga tgtgacaatt tcttttaata ctgtcgcaac atcttctcta ggaatttcac
     4141 ctttaccatc aaaatattgt gcagcttcta tctttccaga tcctgctgca tttgtaagtg
     4201 cccctggatg taaaattgta taattcaaac ctgaacgtct taaatagtca tcagcgtaat
     4261 gtttagctat tgtatatggc tttaaatcac cgctatcatc aaaagcctga cgtctcgaat
     4321 catatgttga aaccatgaca tagtgtttaa tattggcctc tttactcgca atcattgatt
     4381 taacagcacc atctaaatcg acaataattg ttttatctgc acccgtgttc cctccagaac
     4441 ctactgaaaa gataacttta tcgaatggtt taaacgtctc agttaaagtc tctattgaat
     4501 cattttcaac atcaacaaga attgctttca taccttgtga ttttaacgca ttaagttgat
     4561 ctgattgcct aacaccagca gtaaatggta cattttcttt tgctaattgt tgcactagta
     4621 acgaacctac accgccatta gcacctataa ccaaaatatt catttacaac actctcctat
     4681 ttattattct ctatgccata ccactttatg agatatgtaa aacttgttac aactataaaa
     4741 atcaattgac atactactgg gaacgtatta aattaatata tgaacaaata ttcacatgaa
     4801 aggattgtca tataatgtca gaacaatatt cagaaataaa tacagataca ttagaacgcg
     4861 taactgaaat tttcaaggca ttaggcgatt acaatcgaat acgtatcatg gaattgttat
     4921 cagtcagcga agcaagtgtt ggtcacattt cacatcaatt gaatttatct caatcaaatg
     4981 tctcgcacca attaaaatta cttaaaagtg tgcatcttgt gaaagcaaaa cgacaaggcc
     5041 aatcaatgat ttattcatta gatgacatcc acgtagcaac tatgttaaag caagccatac
     5101 atcacgcgaa tcatcctaaa gaaagtgggt tataatatgt ctcattcaca tcatcatcat
     5161 gaccatatgc atagtcatgt aactacaaat aataagaaag tattgtttat atcgttttta
     5221 ataatcggtc tatatatgtt tatcgaaatc atcggcggtc tccttgctaa cagcttggca
     5281 ttactatctg acggtatcca tatgtttagc gacacattct cattaggtgt tgcacttgtc
     5341 gcatttattt atgctgaaaa gaatgccaca actacaaaaa catttggtta taaacgtttc
     5401 gaagtactcg cagcgttatt taacggtgta acgctttttg taataagtat tttgattgtt
     5461 tttgaagcga ttaaacgttt ctttgttcct tctgaagttc aatcaaaaga aatgttaatc
     5521 attagtatta tcggtttaat tgtcaatatc gttgttgcat tctttatgtt taaaggcggc
     5581 gacacttcac acaatttaaa tatgcgtggt gcttttctac atgttatcgg agacttatta
     5641 ggttcagttg gcgccattac tgcagctatt ttaatttggg catttggatg gacaatcgcc
     5701 gatcctatcg caagtatttt agtttccgtt attattttaa aaagtgcttg gggtatcaca
     5761 aaatcttcaa ttaacatttt aatggaaggc acaccaagtg atgttgatat agatgaagtt
     5821 ataactacta ttaaaaagga ttcacgaata caaagtgtgc atgattgcca tgtttggaca
     5881 atttcaaatg atatgaatgc attgagttgt catgttgttg tagaccatac attgacaatg
     5941 aaagaatgtg aattattatt agaaaacatt gagcatgatt tattacattt aaatattcac
     6001 catatgacta ttcaattaga aacgcctaat cacaaacatg atgaatcgat tatatgttca
     6061 ggaacacata gtcattcaca taaccatcat gctcatcatc acgcgcatgt acattaataa
     6121 ttttaaccta ctgccattgc atcgattaaa cttttcaatg gcagtaggtt ttttatgtct
     6181 ttatggcgac ttgtttggtc tttgatgatg caatgtttat taacaaattt tcaactatta
     6241 tttcttacat tagtcatatt tttgacaatt tactattata attctctaac tttagtcact
     6301 ttaattaatt tttattagat attaatatga aaataacgtg ttttttgtta ttgtatttat
     6361 atgaagaaaa ggaggcacca ctttgtttac ttactttaaa tcagcattta aaaatgctaa
     6421 accacaatta ttaataacat tgatatatgc acttatagca tttgctgtaa ttgcagttgt
     6481 atatttactt gctaatttcc agcttgctaa gtatgcacaa actattgcaa tttattcaca
     6541 atttgggcaa aaaccaccag tagatgcata tttaaaagtt atcgctgtat tacttatagc
     6601 agctgtagtt tcactgtttg ttttagttca aatttttatc ggaattacaa atgttatgaa
     6661 acgagcaatg agtcatgaaa aagtaaaatt taccgattta tttattgcat ttaaaaaagg
     6721 taattattta aagagcgttc tgatcggtct tgtatctata gcaatgatta tcgttctttc
     6781 acttctgaca tcattactat acaaattatt ttctccagta tcagaaatga ttatgaattc
     6841 agtacaatca tcatatgcag atagtacaca cctgattggt attgctatta caactcaaag
     6901 catcataatc attgttgttt tattgattaa agcaattatt acttggttat tgttaatacc
     6961 tattttcaac tttatgacta gtttcgttga atcaactaac gataaagtaa aaacacactt
     7021 agctaatgga tttaaagcaa tgaaaaatgg tcaaaaaaca tttttcaaat ttttcattgg
     7081 aatactatta ttaaacttaa ttattatttt atttaaaaca cctgttggtt acttgatttc
     7141 atttaatacg caatcactct ctcaaagtgt tgcagaaaat atcataagag tttatacagt
     7201 aatgacaatt attttattcg ttgttattca tgcaatcatt ttaatgggta tcgttcaata
     7261 ttacttaaaa cgtggtcaaa aaattactaa agataaagta aaaacagctg acaagaataa
     7321 aaaagttgtc actgaaccaa aaaatacaaa agtagaaaat gataaagtga caacatctgt
     7381 tgaaacaaaa acagaaaaag cacaagattc attaaatgat aatacaacaa aaacaatgac
     7441 ttcagataaa ccagaagaca atcaaccaaa ataaatttta gagcccctta caactttagt
     7501 tgttaggggc tcttatgcat gcgcttagcg caggcgctcc tacttttaaa ctttagttgt
     7561 taggggctct tatgcagttg atgcgtagca tccaactgtg ttcctctatt tttcaacttt
     7621 agttgttagg ggcttctttc taaaataact aagtgaatta tctatatatg aagaggtctc
     7681 caatactttt tatataattc gtgattacat tctatacttt taattgtatt ttctgctgta
     7741 tggaaagttg gttttattaa ttctgattat gttcagaact acaattcata agaaaaatgt
     7801 gttaaaatat atttgtagta agtagaagca aaagatgaaa atcattaact cttgaaacac
     7861 aaaaagggca acactcggaa acatgttacc ctaatgagcc cgttaaaaag acggtgacct
     7921 tatattttat ttaaaaatag ccttcaaaaa tgccggtcaa agcgaataga aggttatttt
     7981 ttatggtctt gagtactaat caatactaaa ccaatgattg ttaatacaac cataagcatt
     8041 tcaaaatcag acatgctagt cctttctagg agtaacaact ataaccatag gcatcacccc
     8101 tttattaaga gattagccac catctatcca acttgctcaa taaatattat aacatgcaca
     8161 cttttaaaca gtaattcata gtataagttt tttcatttct taatcaactc gataaacagt
     8221 tgagtgacat acccttgtct aactagctat ttcttcccct gttatcgttt gttcatcata
     8281 taaaaattta atttctcatt ttttattctc tggtaaagag gggcgcccgc tttttcggcc
     8341 tctcgctctt gttgctgcta agtctatttt atacactcac atagtaaatt aacttctaat
     8401 cctgaaaaat gcactcatca tcgtaaaaaa cattttgccc attgtgtctt tcgttgaaat
     8461 gttcatgtta ctaatttgta agtcaattcc gttatcatcg aatcattgtg ataattcaat
     8521 taatagtttt gtcgttctac ctaaccgatc aagttgatag acaactaatg tgtcgctctc
     8581 tcgtaaataa tctaaacact taacaagctc tgtacgtttg acaccactta ttctttcact
     8641 atatacacac ccacacccaa attctttaag tgtatcgata ttaatctttc gttgatacac
     8701 gatcataacc aattttagcc atttaaataa aacctctctt caacatatta tatttagtta
     8761 tgactcaaaa ctcattgccg tatttttgat tttgtattaa tatatcaagg ctatcatcat
     8821 ctaataccaa atgaatttct ccacttcgaa tatcaatagc aacgtttatt tttgattcta
     8881 actttttcaa ataatctatg tcagtaaata tgtacttaga aagataatca tcttcttttt
     8941 taaattcttc tttatcaatt gtatatccgt tctctaaaat ttttgataac tctataagag
     9001 tattactcct actttctttt tttacattag acactacata gtttatgaac ttttcaatca
     9061 attgttcttc caaatcacta taatcttgtg tgtccttttt aaataattca aacataatat
     9121 tattggataa attttcatta tatattaatc gctcgtacat accagaagcg ctagagttga
     9181 taaatctatt cagatcgttt tgtatttcgt tcaaatctat attcccttta ctatcgtaat
     9241 atgaataatt actcattaat ttttttaaga aatctctcaa accaaagtaa agataaatat
     9301 ttgaatactt tctggcatta tcgtagtctt ttacatctct tttatagtga tctatcaaca
     9361 tattcaattc gaaattatat ccatgattaa aatcaaactc gccattgtca tattttcttt
     9421 gaaattcaaa cttgtatatt tctgcaactt tatcatcgca attttcatct atatagtttt
     9481 caaccaaata ataagcacgc tcaagagaaa tttcaccata actttgtata aaagaggtta
     9541 agtactctgt tttctttaaa atttcttctc ctttcttagt tggtatatat acatatggta
     9601 aatctaagtt attaataata tgaaaattgt cgtcaatacg tttaattaag tcaggtttat
     9661 tacctgttgt ttttatacca aaacttttta aaatactttg taattcaggt cttgtctttt
     9721 tatatatact aacttcaaaa tcattagtcg ttgttaataa ctcagcttca attaattttt
     9781 ttattgaatt cataatttga acatcattat tagcaaggca cttattagca aatatctctt
     9841 ctccaaccaa tctatttaca ttagtgtgta atactaataa atcacgtgca tttacgctca
     9901 ctagctttca tctcctcatt tttgcttttc aaatttatta agtatgaata aaaaatacat
     9961 ccgaacaaac aaaatctcta tattctaatt cgatttacat agattataca ctaaaaaaat
    10021 gattctcaat gcaaatgaat gtgtctctca tagtctaaaa actcgtatgt ttcgaatttt
    10081 atgattcatt gataatagta ccagttaaaa gaactaagat tgtaattatc cccccttaaa
    10141 attcaagaat atattttctt aatctatcag caagaggtta tatttcaaag tgtttatttt
    10201 taaaacaaca tctaaaagcc attttcataa taatgttaaa tcattagaat gtataaagat
    10261 tccaattaat aaaaatagat aaaatgcaat aaagttccaa atacttatta ttattcatca
    10321 caaattacgt gatgccctct acaacactaa atcaaggatg atatttattt tatatacatt
    10381 agcattcatt gtttgcttac ccacattatc ctgtttagtc ttttttataa ctctagtctt
    10441 gaatgtcata tttacaaaga aaaaaacact aaaatattta aaactaacac tatttattca
    10501 ctttgttctt ttatagcacc ttttatgttt attt
//
LOCUS       DANJFZ010000092        10452 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.092, whole genome shotgun sequence.
ACCESSION   DANJFZ010000092 DANJFZ010000000
VERSION     DANJFZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.092"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..102
                     /locus_tag="PWN45_002041"
     CDS             <1..102
                     /locus_tag="PWN45_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDL0623791.1"
                     /translation="NGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(426..1712)
                     /locus_tag="PWN45_002042"
     CDS             complement(426..1712)
                     /locus_tag="PWN45_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0623792.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            1912..2010
                     /locus_tag="PWN45_002043"
     CDS             1912..2010
                     /locus_tag="PWN45_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623793.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2253..2429
                     /locus_tag="PWN45_002044"
     CDS             2253..2429
                     /locus_tag="PWN45_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623794.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            2693..3073
                     /locus_tag="PWN45_002045"
     CDS             2693..3073
                     /locus_tag="PWN45_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0623795.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3070..3966
                     /gene="pmtA"
                     /locus_tag="PWN45_002046"
     CDS             3070..3966
                     /gene="pmtA"
                     /locus_tag="PWN45_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDL0623796.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            3967..4647
                     /gene="pmtB"
                     /locus_tag="PWN45_002047"
     CDS             3967..4647
                     /gene="pmtB"
                     /locus_tag="PWN45_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDL0623797.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            4644..5516
                     /gene="pmtC"
                     /locus_tag="PWN45_002048"
     CDS             4644..5516
                     /gene="pmtC"
                     /locus_tag="PWN45_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDL0623798.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            5516..6256
                     /gene="pmtD"
                     /locus_tag="PWN45_002049"
     CDS             5516..6256
                     /gene="pmtD"
                     /locus_tag="PWN45_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDL0623799.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(6322..6885)
                     /locus_tag="PWN45_002050"
     CDS             complement(6322..6885)
                     /locus_tag="PWN45_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0623800.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            7006..7530
                     /locus_tag="PWN45_002051"
     CDS             7006..7530
                     /locus_tag="PWN45_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623801.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            7590..8147
                     /locus_tag="PWN45_002052"
     CDS             7590..8147
                     /locus_tag="PWN45_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL0623802.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            8144..8986
                     /locus_tag="PWN45_002053"
     CDS             8144..8986
                     /locus_tag="PWN45_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL0623803.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(9043..10083)
                     /locus_tag="PWN45_002054"
     CDS             complement(9043..10083)
                     /locus_tag="PWN45_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL0623804.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
BASE COUNT         3576 a         1369 c         1768 g         3739 t
ORIGIN      
        1 aatggttcgt caaaatatgt agacttaaaa actgagtata aagatgaaag agtatttaaa
       61 gcaactgaca ttaaaaaggt agatattgaa cttaaattct aatctttaat tttataataa
      121 ggcatctcac aatcgtgggg tgccttttac atttgtagag atgtgatgct tgaatgtttg
      181 aagtgatttg ccaaaagtta tataaattgg ttctataata aatcggactg atagaaaaag
      241 ttttttactt ttcatctgtc cgattttttg attttgttag gagagatgta taaaactaat
      301 taggtatagt gtttgacaag ttgctgaata aaatatatct tatgatactt tgaaagaagg
      361 aataatttta aaaataaaaa accaataatc cgagtcatac tcatcagatt attggttgaa
      421 attaattatc ttaagtcatc aatcgcttta gcaattgaat caaatacatg tgggatatct
      481 tctttttcta cacagctaaa cgcaatacga atatctgttg cattaagtgc aataacacca
      541 attgaatatt tttcaattaa atgtttacga agttcttcag ggtcaacatc atgaactttt
      601 attgccataa agtatccaga gttaaagtca taggcttgcc aatgtgaatg atattgatca
      661 gcatagacaa cttccttagt gacttcatag cgttctttta atgtttgaat attttgttcg
      721 atttctttat cgaattgttt attatttttt aatacatgct taacagcgct ttgtgttgga
      781 agaggtccac tagaaatgtt acttcgtata agacctttta ctttggcttc taatacttct
      841 ttagttgttt gatcagacgt tccaaatgtc ataaacccaa cacggaatcc ccatgcgaaa
      901 aattcttttg ttgcaccatc taaacgaaca ggtaatattg catttgaatt taaattagat
      961 aatgcagtaa ataatgattg agtatacaca tcttcataga ataaaccata gtatgcgtca
     1021 tccacaacag ctataacttt tgtaccttta tcagctaatg ctttaattgc atcgacgatg
     1081 gtagtcactt ctttatgcgt aggtgtgtaa cctgtcggat tattaggata atttaaaatc
     1141 ataatgactt tatctttatt gtatgattgt aaagcttcta caagtgaatc agtagtataa
     1201 tgcccgtctt tatcaaagat aggatatgtt tgaaggtttg caccatttct cgtattgaaa
     1261 acaagtttgt aattacccca attatgctct ggtaacaaga tagtgtcacc ttgatttaca
     1321 aataaatcgc caactaaaga taaaccatgt gttaatgcat tcgtaacaat tggtagtgac
     1381 atgttgtcga ttgatagctc tggattgtca cgcaacattt tttgttgcca taaatcacgt
     1441 aattcttcga tgccttgtgg tggcgcataa gggaatattt catctggagt taaatcatta
     1501 aacattgcat ctaaagatga tgcaaacatt tttccgtctt tgtttgtcgc catacctata
     1561 gttgcattat atgttgtact ctttgcttca gcagattgag ataaaatgcc ttttggataa
     1621 aacatatttt gacctaagtc agataacatt gtaaaggcag ttgcatttga ctgttgaagt
     1681 tgttcattta agctttgggc taaaggattc attattgctt aacctctctt taaaaaaatt
     1741 gtatgcctat atattatctt caattatacc aaagataaag tgttgttggt gaattaacga
     1801 ataatatgta aaatctgctg ccaaaatacg aacatatgtt ctgtttttat gttagaatag
     1861 gtgtaggtca tccttcagta tgatacttta gtcgaacgga ggtgtagtgt aatgatgtgg
     1921 tggcaagaag ggatgatgac acttattaca ggtggcttac taattatttt tcgactttgg
     1981 ttagaactga aatggaaaaa taaaaaatga tcctacacac ataaataacc ccaagtctgc
     2041 tggtaccaga cttggggttg tgtagtataa ttctgtggat acacttaaat aatatcattg
     2101 aacacaatat atcgcaatca ataatcattt tgattgaatg gttttgttgc gctattttag
     2161 ttgaaagtga ttagtacctt gaacttttga aattcaataa cttattaaaa aaatttatat
     2221 ttgttataat gtcgatgttg aggtgaaata aattgaaaca tctaacaaaa atatttgtag
     2281 tacttgcaat tattttattt attattggtt attatttaca agcaactaat catgaaagcc
     2341 aaggtataaa attattatta gcagcgatta tgtttatgat ttgtgctttt ataagtagaa
     2401 gtaatgatcg acgtaaaaat agtaaataga attgactgtt taaaacgatt gtttatgttg
     2461 acgctacggt tgtcacgtag agattaaaga cattcgtttt agcagtcttt tttattgaag
     2521 tagacaatgc aagattaaat ggtagtgtca tttcatttta ttttattcaa ctgctagtaa
     2581 tacaatactc agtgttattt ctgaagactg gttgataatg agacatgcat atttacaaaa
     2641 ctgtgtatat tgtgtatatt gtatatatac agaagttgat agggggacgt tgatgaaaat
     2701 aattttaaaa aacaatagtg attttccgat ttatgaacag attaagcaac aagtaaaaca
     2761 aaatatttta aagggacatg ttgctcctgg agagcatttg ccgtcaatga gagaacttgc
     2821 caaagatctt caagtaagtt tgattactac caaacgtgct tatgaagatt tagagaaaga
     2881 cggttttgtt acaacaatta gaggaaaagg gacctttgtt aaggagcaag atagttctat
     2941 tttaaaagag aaacaatttt ttaccattga aaatttggtt aaagaattgg ttaatgaagc
     3001 gcaagccatc gaaatgtcac ttgaggaact gcaggatatt ttaacgttca tttatgagga
     3061 ggaatcatca tgaatgccat agaattaagt aatgttaatt attcaagtga tcagtttaat
     3121 ttaaagaata tctctttcaa agttcctcaa ggattcgtca ctggttttat tggtagaaat
     3181 ggcgctggta aaaccacaat aataaggtta attatggatt tgtatcaacc acaaactggt
     3241 gttattagag tattagaaga ggatatggct ctcaatccga tagagttaaa gaatagaatt
     3301 gggtttgttt actctgaaaa ttattttaat gagagatgga ctacgaagca acttgaaaaa
     3361 atgattgccc ctttttatcg taaatgggac catcaagtat ttgaatttta tcttgaaaag
     3421 ttcgatttac caatcaataa atcaataaaa acattttcga ctgggatgaa aatgaaatta
     3481 tcactagcag ttgcattttc acatcatgct gaattgtata tttttgatga gcctacttca
     3541 ggtcttgatc ccttagctag aaatgagcta ttggagataa ttcagcaaga attaattgat
     3601 gaaaataaaa caatattcat gtcgacacac attatctcag atttagaaaa aatagctgat
     3661 tatatcattc acttaagtga cggtgaagtt atcttaaatg gttcaaaaga acaactgtta
     3721 caaagatatc aagttgtgag tggtgctatt gaggaccttg atgatgaatt agcatctcta
     3781 cttatttatg aagaacataa gcgtacggga tttataggat taacagaaca tgcgcaagta
     3841 tttaaagaaa ttttaggtca taaagtgaat atcactacac cttctattga gaacttaatg
     3901 gtttatcttg aaaagagaaa acctaaatat catgaaaaca tcaaattgat ggaagaaggt
     3961 ttttaaatga aggcattgtt aattaggaat tttaaattac gacgatatac acttatcatc
     4021 tatgttctat tattaacctt atatcctttt tatataatgc ttgactcaac aaagttcttt
     4081 tacttattgc aatcttttat aagtccaaca atattgatta tttggatttt agatgctggt
     4141 cacttattta gactcaatcg tagattaggt ggtaatgatt catattattt ttatatgagt
     4201 ttaccagtgt caaaaaaaca attgcttaat gccaactata ttacatgcat tgttttaaca
     4261 ttaattggaa cgcttgttat tagtttatat gcttatgaag cagatgtgat tgaaccaaat
     4321 tcaatctatt tttcaactgc atatgcattt gtcatatcta acttcttgtc tataccaatt
     4381 gcatttagtc aatttacaga attgcgtaga gtcaaagtgc catatggtat atacgtgttt
     4441 actattatca ttttagttcc atttttattt tcaattgcaa tagtattggt gaattatttt
     4501 gttctaagtc aatcatcatt cccagattta tattcatata ttttaaatat tggttttcta
     4561 attataagca ttgttatact tattgttaat tattttaaac agctcaataa aataaatact
     4621 agaaaattta aaggaggcag tcgatgaaat tagaacatat tacaaaaaaa tacggctcaa
     4681 atgtcgtttt aaatgatatt gattttgact ttggcgatag tagaattgtc ggattaatag
     4741 gaaaaaacgg tgttggtaaa acaaccgtta tgaaagtaat gaatggtaat attattaaat
     4801 ttgatggaaa agtagatatt gataatgcag ataatatcgg ttttttaatt gagcatccta
     4861 aattatatga taataaatca ggattgtata acttgaaatt atttgcacaa gtattaggta
     4921 agggttttga taaagcatac acagacaaaa ttatagatgc atttggtatg agaccttata
     4981 ttaaaaagaa agttaagaaa tattcaatgg ggatgaagca aaagttagca attgcagtat
     5041 ctttaatgaa taaacctaaa tttttaatct tggatgagcc tacaaatggt atggatccag
     5101 atggctcaat tgatgtgctg actacaatta agtctttagt aaatgaactt gatatgagaa
     5161 ttctaatatc aagtcataag ttagaagata ttgaattaat ttgtgataga gctgtatttt
     5221 taagagacgg acattttgtt caagatgtaa acatggagga aggtgttgca tctgacacaa
     5281 cgatagttac tgttgatcat aaagactttg atagaactga aaaatatctt gcagagcatt
     5341 tccaattaca aaatgtcgac aaagcagacg gacatttaat gatcaatgca caaaaaaatt
     5401 atcaagttat actaaaagca ttatctgaat tagatattta tccgaaatat attgaaacac
     5461 gtaaaagttc attgcgtgat acgtacttca atataaatca aagaggtgat aaataatgag
     5521 aattttaaat ttagttaagt atgattttta tagtatattt aaaagtcctt taacatattt
     5581 agcgatacta gtcgtatcta gtttgattgc aactcaaagt atacttatgg caaattcgat
     5641 ggataacccg aaacatatta ttgtctatgg atctgtattt gctgcagcaa aatggttatt
     5701 gttaataatt ggattaatgt ttgttgttaa gacaattacg cgtgattttt cacaaggtac
     5761 aattcaacta tatatgagta aagttaaaac acgcgttgga tacattattt cgaaaacaat
     5821 ttcaattatt ttaatttcaa tattatttgc attaattcat tatgtgattt tgattgttgt
     5881 gcaggcatct agtaatggaa aaaatttggc gttttctaaa tatgtagata atttatggtt
     5941 cttcctaatc tttttactat tctttggctt gtttttattc ttaatcacac ttgcatcaca
     6001 aaaaacagca atgatatttt cattaggtgt atttttagta ctcattgtac cgtttattaa
     6061 accttttatt acatttatcc caagatacgg tgaaaaagtt ttagatgctt ttgattatat
     6121 cccttttgct tacttaactg ataaaatgat tagctctaac tttgatttta gcaattggca
     6181 atgggtaatt tcattaggtt ctatagtgat attcttcatt ttgaatatct tatatgtcgc
     6241 taaaaaagac atttaataaa aataattttg aggttgggaa ttttaaattt tcccaacctc
     6301 aaagtttgtc ttattgtaaa tttatttatt ttctaattta tttaggatgg aattataaac
     6361 tgctttccaa aatgaagcgt cagttttata gcggtttgat ataactaagt gtgtttcttt
     6421 ttctaaatct gcatagtctg gatgatcttt gctcggtaat ttatcagcac gaacatcagt
     6481 tacaaatttt tggacttcat ttgctcttgg tccccaaact gtttcttgtt cgaattgatc
     6541 attcaagaat acgaagatag gaattgcacg tgatttacca tttgttaaat attgatcgat
     6601 cagttttgta tcatcatctc tatggaacac gcgtacttct aaatttaatg cttcactgat
     6661 gtgttttaga attgggagat tcatcattgc atctccacac cagtcttcag taattactaa
     6721 tactttagaa taattcatct cttttatttt tttgatgcgt gaatcatctt ctggtaactc
     6781 aaatgattga tagatactga gaacggtatc ttgatttgtc ttcattccat caatgtattc
     6841 atttaagggt tggctatttt tgaaataagt ttctaaattt gtcattgtaa aaacctcctt
     6901 tagcatttac aacattatac caatttatag taataaaagg taatgaaata aattaattgc
     6961 aaattctttg ttaatttttg ttaaggatga aacgggaagc accttatgct atatttaaat
     7021 aagtacaaag aaagggtgac atcagtgcgt attcaaaatc gctgggttgt gtttatatta
     7081 tttttaatct gttcttttgg tgtattaatt ggtttatatc aatatcgtca tacgaaaact
     7141 gtggatttgt ctaatcttga aataaacgat attaaattaa atgagaagtt tgataaaaaa
     7201 ggctatgaag taaataaaaa aattaagttt gatcgtttta aattttataa cagtaaagct
     7261 caccctgatc ttaccgttaa agtgagagaa aaggataaca tcgttaaggg gataatatta
     7321 gtaagagatg aaaagataca tactaatttt gatgggggaa ttggttcgcc gataaataac
     7381 gcgattgaaa atcttggatt cggatataaa agaacaaaag ttggcaatga tttctcatcg
     7441 gtaaagtata ttgatagaga taaccattta aaattaaact tactttatca agatttagaa
     7501 attaaacgta ttgaattttt tagtaaatag ctttaggtct taaagttata aaaaacgaat
     7561 gaataatttt attgggatga gtgacctata tgaacaagat tacttattta aatgaattag
     7621 aaacagcact tgatgcgcta cctaggcatt taagagatca aaaaatgtat gaatatgaac
     7681 gttattttta tgagcaagaa cttaatggcg ttgatgaagg ggaaatttta aagaagttaa
     7741 aagacccaca agatgttgca gctgaaacaa aagctagaag tgtcattgat tatgctgaat
     7801 ctaaaccaac atttgaaaat atttcaagag ctgttgctgc ttcattaagt ttaggcattc
     7861 tatctatttt tgtcatcctt ataccagtat ctatagttgg attatttgta ttagcattat
     7921 ttttaatatc acttttgctg ctgttttgtc caattatttt attagcatca gcaatatcca
     7981 gaggaattgt ggactcaatt agtaatgtat tttttgccat atcatattca ggattaggat
     8041 tagtatttat cattgtcata tttaagattt tagaatacat ttatcgttta atcttaaaat
     8101 atttactttg gtatattaaa actgtcaaag gaagcgttag aaaatgaaga aattcttttt
     8161 tattgggctt ttagtgtttg ttgtcttttt tacagcagca accattattt ggttcagcta
     8221 tgataaaaac aaatatggta ctaaacaata tgataaaaca ttcaaagacg atgcttttga
     8281 caatgtatct ataaatttgg atagtacaga acttcgtata aaacggggga atcaatttag
     8341 agttaaatat gatggtgaca atgatatatt aattaatata gtagataaga cgttgaagat
     8401 tagtgataaa aggtctaaga caagaggata tgcaattgat atgaatcctt ttcatgagaa
     8461 taagaaaacg ttaacgattg aaatgcctga taaaatgatt aaacgtttaa atctatcatc
     8521 tggagcagga agtgttagaa tcagtgatgt tgatttagag aacacaagta ttcaaagcat
     8581 taacggtgaa gtagttatta aaaactcaaa tcttgacgca ctagattcaa agacaaataa
     8641 tagttctact tatattagta aaagtaacat taaaaatagc aatattaaag ttgttattgg
     8701 tacgctacaa atcgacaaga gtcaaattaa acaatccata tttttaaacg atcatggtga
     8761 cattgaattt aaaaacatgc catcaaaagt agatgcaaaa gcttctacta aacaaggaga
     8821 tattcgtttt aagtatgata gtaaacctga agacactata ctaaagctaa atccgggaac
     8881 gggtgatagc gtagttaaaa ataaaacatt tactaatggt aaagttggga aaagcgacaa
     8941 tgttttagaa ttttatacga ttgatggtaa tatcaaagtt gaataaataa aggatgtaag
     9001 caccgatatt aggaagcata atttctctaa tatcggtgtt atttatttgt tggcaaaagt
     9061 taagtcggta tctatattgc cagtaaagtg agtgatatta aggtcttgac catctaacca
     9121 tgatttgaaa tctattattt ctggtggcgc attttctccc aatgtaaaat atgcagttaa
     9181 tgtttcaggt tgatacattg atgtatggat ggtgccagac cagcttttga atagtttact
     9241 gtaaatttca tactgaggat tattgaataa cttaaatgct gtagtcatat ctaaattatc
     9301 attagtttgt gaaatggtac gcgccagtct ttctttagat tcttttgtat aattacgatt
     9361 ttcatgtgtt aatatttcaa aatgatttgt acatatatta tcataacgaa catctattga
     9421 tctcggtgtc acttcaacaa ttgcatggtt caatgatttg tccatcagta tgtagctaaa
     9481 tgagcttctg tgtggtattt ctttcaataa ttggattgct tctgttacat ttcggcaatt
     9541 ttcaagaatt agacgaccaa tcatataaca tacaaaacca tttgctggtt tcttccggtg
     9601 cataaagtta tagcccatag ttaatcctga ctcattcata ccatccattc ttccagttac
     9661 ccttgataca ggaccaattt gagctaaacc gctatctgta ggttgataaa gtaagtagcg
     9721 accatcataa gttgcagggt ggtaatcata atttctaacc atgaagtctt tgccttgaaa
     9781 gaccgtgcaa ccactttctt ttaaatcggt aaaacgataa tgtccaaagt ttaaaataat
     9841 ttggcgtgtt ggcattttga gtatactttg tagtcccatt aattcttccc atatttgagg
     9901 tgcgtatgtt tggaatattt gataagtttc atttacatct atatcgaaac gtgggacacg
     9961 ttttttccat tctttttctc gattttttag aagaggtgtt tgttgaagcc atttaccagt
    10021 tttaacacct aactcgaaat gtgaacctct aaaagtcatg atatctgatg tcacttgttg
    10081 catatcatcg gcccctttct ttttagttgt aatatattgt aaataaatag taatcgtatg
    10141 tatattgaat gtcatgttaa ataaagttat attttactaa atgaaatata aaattgtttg
    10201 aggtgatttc tcggtgtata agacttatca atcagttaaa acatattttt atagatggtg
    10261 gggatattga gttaaaaact taaaatcatc ttatcataaa tatcaatctt aagttagcat
    10321 tcacgataat agtcattgtt aacattagca tataaggtca tgtcacgttg aaacagaggt
    10381 tcctcggcat ttttgaaaat aatcattagc ttgctcacta tataatttga tgaatatatt
    10441 tcgtgaaagt gg
//
LOCUS       DANJFZ010000093        10220 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.093, whole genome shotgun sequence.
ACCESSION   DANJFZ010000093 DANJFZ010000000
VERSION     DANJFZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.093"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            192..482
                     /locus_tag="PWN45_002055"
     CDS             192..482
                     /locus_tag="PWN45_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623805.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            482..2269
                     /locus_tag="PWN45_002056"
     CDS             482..2269
                     /locus_tag="PWN45_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL0623806.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            2503..3852
                     /locus_tag="PWN45_002057"
     CDS             2503..3852
                     /locus_tag="PWN45_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0623807.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            3874..5502
                     /locus_tag="PWN45_002058"
     CDS             3874..5502
                     /locus_tag="PWN45_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0623808.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6024..6374
                     /locus_tag="PWN45_002059"
     CDS             6024..6374
                     /locus_tag="PWN45_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623809.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            6461..6772
                     /locus_tag="PWN45_002060"
     CDS             6461..6772
                     /locus_tag="PWN45_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL0623810.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            6787..7293
                     /locus_tag="PWN45_002061"
     CDS             6787..7293
                     /locus_tag="PWN45_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL0623811.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            7345..8952
                     /locus_tag="PWN45_002062"
                     /pseudo
     CDS             7345..8952
                     /locus_tag="PWN45_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(8943..9203)
                     /locus_tag="PWN45_002063"
     CDS             complement(8943..9203)
                     /locus_tag="PWN45_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDL0623812.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(9175..10161)
                     /locus_tag="PWN45_002064"
     CDS             complement(9175..10161)
                     /locus_tag="PWN45_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDL0623813.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
BASE COUNT         3831 a         1751 c         1619 g         3019 t
ORIGIN      
        1 taatcaacta agtttccata cttatcaata atttcttcta gcgtaggtat caatatatac
       61 cccccctccc cattttttgt tccgcgtcta atcataatgc ttcatttatg cttattatag
      121 caatcaattg aagatagttt aacctatatg atattgattg actccttaaa aattattgga
      181 ggaattttaa aatgaataag aaatctaagc agcaagaaaa gctttataat tttataattg
      241 ctaaatcttt tcaacaacca gttggaagta cgttcactta tggtgaatta agaaagaaat
      301 ataatgtggt ttgtagcacg aatgatcaac gtgaagtagg acgtcgtttt gcttactgga
      361 ttaagtacac gccaggatta ccatttaaaa ttgtaggaac aaaaaatggc agtttattat
      421 atcagaaaat agggataaac ccatgcaata atagcacgcc ttcgaaagga ggtgattgct
      481 aatgagttac acattgtttg aaattggccc atttgcttta gattccttcg gttggaaaga
      541 aacgatacca cctaaaaaag atggtgacag tgaaaaagtg gttagaatgg ctagccctat
      601 tattgttaat gcaagatttt ctgatccaat tactggtgtt gaaaagttga ttattactaa
      661 caacaatggt aaaaaagaca tttttgagtc tgatatctta acaacacgaa acttaccttc
      721 tttaattaaa tatggctaca gtattaacga gaagtatatt agatcattga gttatgcctt
      781 gcaattgatg cgagatagat taccactttc tgaattatat gagggtgtag gtatactaga
      841 gacacctttt ggctacttga tatcgctaga taaagttctc aaatctattc aatttaatca
      901 gtcctcacct tcatacccta ttgtagatag tgcttatgac ttaactccta aaggtacatt
      961 tgataattgg tttaacatgt atattgacga ggttaaaggt catcttcttt tagaattggc
     1021 agtcatattt gggatttctg ccttagtgac aagttttctg aagcataaac atgaaatcga
     1081 atttgcagga attctcttta gttttacagg tcaatcctca accggtaagt caaccgcagc
     1141 tgctttagct gtatcagtcg ctggtaaccc taccaaaggt aatgaaactc tttttagaag
     1201 ttggaatgcc acacgaaacg cacttgaagg ttatttgagt aacaattatg gcattcctat
     1261 agtatttgat gagctttcat ctacgacact tagagatacc acgggtttgt tatattccat
     1321 cgctgaaggt caaggtcgtc aacgttcaaa cgttcatgga gaagttaaaa cgcctaagaa
     1381 ttggggtact tctgtcattt caacgtcaga atacagtatt tttaatgatt cagctcaaaa
     1441 cgatggatta cgtgtaagaa ccatcgagat taatgaacaa ttcactacaa atgcgaccaa
     1501 tgctgacaat attaaaaaag ctgtagcact aaactatggt catgttttac cgttagtagc
     1561 taaatatctt ataaatcgtg aagatgaagt tattcaatgg ttttacaagg aagtagattg
     1621 gtttgaagct aaattgaaag atgaaacaaa caatacaggt attcgtatgt ttaagcgtta
     1681 tgcagtcatt acaacatctg cgaaaatatt aggtcgtgtt ttatctacag atattgatat
     1741 cgctaatata agagactact ttattgatta tcatactcat acagtctctg aacgctcatt
     1801 agctgataaa gctattgatg tcatcattca atttgtggca caaaacagag gtaagttttc
     1861 agatgaaggt gctttgaaaa atatgtttga gaactatgga ttaatctcat taaaagatga
     1921 ccatatcgaa gtaaaaataa ttgcgaacgt ttttaaacag atgctaaaca atcatcaatt
     1981 tcaagatgtt aataatgttg tcaacgcctt aagagataaa ggtttcatat tagctgaccg
     2041 tggacgccaa acaactaaaa gaagtgtcaa agataatagc ggtaaaaaac aatcacttgt
     2101 attttaccat ctaaaattag atgtagagtt tgcatctatc ctcggattaa ctaaagataa
     2161 gtcgttactt caaaattgga caccgtcaaa tgataacaaa gctgcgaaag aactttttaa
     2221 atccgctaat gaagggatag gcccttcagg agttcacgaa gatttctaat tgtctttatc
     2281 atacaactgt gaagcatcaa actgaataat cgacgtattg aagatagaac aaataacgga
     2341 gtgaaaaaca tggatgaact tatcgagata ttagccgatt tggtaattga agaaatcaat
     2401 caaacaaatg actaatatgg acgcccaaca tctcattgat gttgggctta tatatcaatt
     2461 catccataaa tatatattat cactgtgaaa ggaggagccg atatgaaaca agtcataggc
     2521 tatttacgtc aaagtacgat gaaacaacaa tctcttgcag cacagaaaca agctatcgaa
     2581 gcaatagccg aaaaacatca tattcaacat atcaactttt atagcgacaa acaatcaggt
     2641 cgcaaagata atcgtagtgg gtatcgacaa ataacacaat taattcaaca aggacagtgt
     2701 gacatattat gctgttatcg tcttaatagg ttgcatcgta atctgaaaaa tgcattaaaa
     2761 ctcatcaaat tatgtcaaac gtaccatgtt catatcttaa gcgtacatga tggttacttt
     2821 gatatggatc aagctttcga ccgactcaag cttaatatct tcatcagttt agccgaactt
     2881 gaatcggata acattggaga acaagtcaga aatgggcttc aagaaaaagc aaagcaaggt
     2941 cgattgatta caacacatgc accttttggt tacgaatatc acaacggaac attcatcatc
     3001 aatcaaaatg agtcaccaac ggtaaaggct gtattcaatt attacattaa aggtcatggt
     3061 tataagaaaa ttgcacagtt attagaagaa gataacacgt atatcaatcg acaaccctat
     3121 caagttcgta acattatcat caatcctaat tattgtggtc gtgtcaacaa tcaatatggc
     3181 caattcgaca atatgtttcc ttctattgtt tccacaagta tatatgagca agcgcagaga
     3241 cttcgattgc aaaaacaaac caaacagaca ccttcggata atcaactcaa acaaaaaatc
     3301 aaatgcccat gttgtaatgc aacacttaca aatatgacca ttagaaaaaa gaatcataca
     3361 ttacgttact acgtctgtcc taaaaacatg aatgcttcac gctttgtctg tgattttaaa
     3421 ggcatcaatg cacaaacact tgaagataaa gtattagaag tgtgccgaga cttttatcaa
     3481 aatcaacgca tctacacaaa aattaaaagt gcgattgaca aacgcatcaa aagacaaaga
     3541 aacatagaaa aacatcacac attgactcaa gaacaactga tagaaaagtt ggcacaaggc
     3601 atcattgatg cagaaacgtt cagagagcaa acacaatcat tacgtcaaca accgcaacgc
     3661 actacatcta tcaatgggca tcaaatacaa cacatcattc aaaatattat tcaaaaacgt
     3721 ttcacgttaa acatattgta cccctatatt gaaaccattc acattacgaa agataaaaat
     3781 cttataggaa tctatttcaa aaatgaacca ctcaatattg tcaatcaaac catgcaatca
     3841 tcgattgcat aaatgaagaa aggatgtaac ataatgcaac aacttaaaac aaaacgtgtc
     3901 ggtatctatg tacgtgtatc aacagaaatg caaagcacag aaggttatag tatcgacgga
     3961 caaatcaatc aaatcaaaga atactgtgac ttccatcatt ttgaagttaa agatatatac
     4021 gctgaccgtg gtatttcagg taaatctatg aacagacctg agctccaacg tatgttgaaa
     4081 gatgcaaaag aaggcaatat cgactgtgtt atggtataca aaacaaaccg attagctcgt
     4141 aatacatctg atcttctcaa aattgtcgaa gatttacaca aacaaaatgt cgaatttttc
     4201 agtttgtcag agcgtatgga agtcaatact tcttctggta aactcatgtt acagatactt
     4261 gcgagtttct cagaattcga acgtaataac attgtcgaga acgtatttat gggtcaaacg
     4321 agacgtgccc aagaaggcta ttatcaaggc aatttaccac taggttatga caaaatacca
     4381 gatagtaaac acgagctaat gattaaccaa catgaagcta atattgtaaa atatatattc
     4441 gagtgctatg ccaaaggaca tggctatcgt aaaattgcca atgcattgaa tcacaaagga
     4501 tatgtcacta aaaaggggaa acctttcagt attagttcaa tcacatacat cttagctaac
     4561 cctttctata tcggcaaaat tcaatttgcg aaatacaaag attggagtga aaaacgtcgt
     4621 aaagggctga atgataaacc agtgatagct gaaggtaagc attcccccat tattaatcaa
     4681 gatttatggg ataaagtaca aatgcgtaag aaacaagtca gtcaaaaacc ccaagtccat
     4741 ggcaaaggaa cgaatctgct tacaggcatt attcactgtc cccaatgtgg cgcacctatg
     4801 gcagcaagca ataccacgaa tactcttaaa gacgggacta agaaacgtat tcgttactat
     4861 tcatgtagta attttcggaa caagggttcc aaagtatgtt cggcaaacag tgtaagagct
     4921 gatgtgattg aagattatgt gatgaagcaa atacttgaaa tagtcaaaag tgataaagtc
     4981 attcaacgcg tagtaacaca cgtcaatcaa gaaaatcaag ttgatggcgc tgcacttcat
     5041 cacgatattg cttataagca acaacaatat gatgaagtac aaatcaaaat aaataacttg
     5101 attaaaacca tcgaggataa tccggactta acatcagtaa tcagaccaag tattcaaaaa
     5161 tatgaaaagc aactcaatga cattacgaat caaatcaacc aactcaaaaa tcaacaaaat
     5221 gaagataaga ctttatttga tgccaaagaa atcagtaaac tattacaaca catctttcat
     5281 gatattaagc acatagaaaa atctcgactc aaagcattgt atctatcagt gattgatcgc
     5341 attgatatta aaaaagatgg taatcataaa aaacaattct atgtcacact caaacttaat
     5401 aacgaaatca ttaaacaact tttcaataat aaacaactcg acgaagtgca tctcagcact
     5461 tcgtctttat ttttgcccca aacactatat cttactattt agctaataga atcattgtta
     5521 taacaataat tttcactata ttctttcgct tattttatct aactattttc aagaattgct
     5581 ctttatcaat ttcaataaca cttatctaat gaaacagtta ttgtcgtaat tttttcataa
     5641 ctatacaatg cttatattga aattgctaaa gaacatccca caatgatttt ttatcgggaa
     5701 atgattggga tttgcagatt cgaagaaaca gaaggattgt gcaagcacaa tcccaaaatc
     5761 ccgttacttt ttataaataa ataatatata taaggaagca gagacacaac cctatcatca
     5821 atacttataa ataatgagtt gattgtttgt caaaataggc ggaatttcag gatttccttg
     5881 gcataccaac gtttgtagcg ggtttttata tggaattttc tcttattccc accgggattc
     5941 gtaataaatg acaaatcaaa tatatattat ctcaatgatg tatatgaact acatcactat
     6001 tacactatct aggaggcaat aatatgaaaa ccatcactca agaactcaaa caatatataa
     6061 cccacctatt ccaactatct aataacgaag cttgggaatg tgaagcgctg gaggaagcag
     6121 cagaaaatat tctaccggaa cgttttatta atgattctcc actggtacat ctcacactcg
     6181 agacttatac ctactataat gatgaactac atgagctaag tatctatcca tttctaatgt
     6241 atgccaataa ccaactcatc agtgtcggat acttagacca ttttgacatg gactttctgt
     6301 accttacaga tactcaaaat atcattattg atgaacgtca tttactaaaa caaggaggtc
     6361 aggaccatga ataattggat catagttgct caattatccg tcacagttat caacgaaatc
     6421 atcgacatca tgaaagaaaa gcagaaggga gggaaatagc atgaaaatca atcgatacat
     6481 cacaagagga attaatgaaa gtattccact agaccttcaa atcttacttt ggcacatggt
     6541 agaaaaaaaa gataaccagc ctcatacaga ttacctacac atttttaaat tacaagaaga
     6601 tgagaatata ctctcaatca cacatgaaca agaacaaccc acatacaaat tggaatatca
     6661 ctatacaaac tatgaaaaaa atcaaaatgc attacctaag aaagtctacg tcattcgtga
     6721 agatgacgtg gacgcttttt attatgtgat gcttttacca gaagaatact aaaggaagtg
     6781 acgatgatga atacaatcaa aagtacgata cacacagaag ccatatttag cgatgatgaa
     6841 cagcaccgct atttactcaa gaaaacatgg gatgaaaaga aagctgcatg cacagtgata
     6901 acgatgtatc ctcatttaga cggtgtatta tcactcgatc tcacaactgt tcttatcctc
     6961 aaccaattag ccaattcaga acgatacggt gctgtatatc ttgtaaatct attctctaat
     7021 attagaacac ccgaaaacct caaacatatc aaaaatccat acgatgagca cactgatatt
     7081 catttgatga aagcgattag tgaaagtgac acagtgattc ttgcttatgg tgcctatgcg
     7141 aagcgaccag ttgttatcga ccgtgtcgaa caagtgatgg aaatgttaaa acctcataaa
     7201 aagaaagtaa aaaagctcat caatccagta acaaatgaaa ttatgcatcc actcaaccct
     7261 aaggcacgtc aaaaatggat tttgaaatca tagcaaatac atggaggtca atatggaaac
     7321 aaaacaaatc aacatcgttt tactgtaaat gatattgttg aaacaattcc tgacaatgaa
     7381 ttcgacgaat tcagacatca ccgtggtgca acttcgtacc atcctaaaat gatgttaaaa
     7441 gtgattctat atgcctacac acaatctgta ttctcaggtc gtaaaataga aaaaatgctt
     7501 aatgatagca tccgaatgat gtggctatca caaaatcaaa aaccttctta taaaacaatt
     7561 aatcaattta gagtaaatcc aaaagtagat gctttattag aatctttatt tattcaattt
     7621 tacagtcagt gtgtaaaaca aaatcttata gatgataaag ctatttttat tgatggtaca
     7681 aaaattgaag caaatgccaa tcgatataca tttgtatgga aaaagagtat tcaaaaccat
     7741 gaatcaaaga tgaatgagga ttctaaagcc ctctaccatg aattggtaac caataaaatc
     7801 ataccggaaa ttaaagaaga tcatgataat gaattaacaa aagaagaaat agatttgatt
     7861 ggtagtcact tagataaaga aatcgaagat ttaaaccaac atatcaacaa tgaaaaatgt
     7921 actaaaacaa gaaaacaaat acgtctcaaa agaactaaaa tcaaaaaata caaaaagcaa
     7981 atcaatgatt attttgagcg aaagtatcga tacgaatttc aaaaatctat tttaaaggat
     8041 agaaatagtt attctaagac agattatgat gcgacattta tgagaatgaa agaagatcac
     8101 atgaaaaatg gacaacttaa gccagggtat aatttacaaa tagcaacaaa ttcccaattt
     8161 gttttatctt ataatgtgta tcaaaatcca acggatacta gaacgatgat tccattttta
     8221 aattcaattc aagagaccta cggtcattta cctgaatata ttgtagctga tgcaggttat
     8281 ggtagtgaat caaattataa ggcaattata gatgacttta atcgaacgcc actcataaca
     8341 tatggaatgt ttataaaaga taaaactaaa aaatataaaa gtgacatctt taatactcaa
     8401 aattggaact atgacgaaat taatgacgaa ttcatttgtc cgaataataa acggctaggt
     8461 tttaaaagat atgcctatcg tcatgataag tatggttata agcgagactt caaattatat
     8521 gaatgtgatg attgttcaga atgtcctctg aaaaatcaat gtatgaactt caattcaaaa
     8581 acaaacaaaa aaataatgaa gaattataac tgggaatatt ttaaatccca aattaacaaa
     8641 aagctttcag aaccagaaac aaaaaatatc tacagtcaaa gaaaaattga tgtggaacct
     8701 gtttttggat ttatgaaggc tattttgggt ttcactcgga tgtctgtccg aggactcaat
     8761 aaagtcaaaa gagaacttgg ttttgtatta atggcactta atataagaaa agtagtagct
     8821 caacgagctg aaaataatca aaaaatttat aaaaaagaca atttctatat tatttcaata
     8881 gaaattgtct tttttcactt atccaagaac tttatgtccc ggactcatta tgatttattt
     8941 ttttattgct gacttgccat taattctgct tgctctacaa cttgttccac agccattttt
     9001 tgaagatctg gtggatagcc atatttcttt aataggcgtc taacttgaac tctcatttta
     9061 gctttagcac tgtctcgttt agaccaatca acactcatgt tatccttaac tgttttagtt
     9121 aactcatgtg caatggctct taactcttta tcgcccaaag cttctttagc tgtttcatgt
     9181 gaagctcgat atactacata caatatacgc aacaatacgc ttgtttcgat taggtttagg
     9241 cattgaatca taaagtgcaa tatacttaac acgttcttta atatttgaat taaaacctag
     9301 taaatattgt gctgccacat tatttatgtg ctgagatttt aaaatagagc attttaatat
     9361 cgattcacca taacgtatat gttcatggcg attcaaaatt tttaaaacgt ttctatcaca
     9421 tactttttca cagtcattgt ccatcattgt tttacttata tatagtgcag gattaaacca
     9481 gaatatcatt ttaaaaacaa cataaagctg gttgaatatt aagtcatgac ttttcacatg
     9541 tgatagttca tgtagaataa tatattcaat ttctttgtca ttcatggttt cgactacgac
     9601 agttggtagt acaatttggg atttcactaa accaaatacc atcggattat caatgtttga
     9661 actataacta attgttatat gctttttgta gaactgcatc ttactttgac atactttaag
     9721 tcgttcatta agatatgacg attccaatga cgaactttta ataacatcaa tttgtcggaa
     9781 tgccttaatc atataaaata agcacaacaa actaccaaat acccatatca aaagaatcat
     9841 atacgttata tttgaggtct caaactgatt aacattaatt gctaagtctt tcgtaacaga
     9901 tgattgttga ccatctaaca tatgactaac cgaagaagtc gtgtcagata catttcgatt
     9961 catcatatct tttgaaaatg taaaattcga tattttgtaa aatggtatta atggaattaa
    10021 cgtggagacg agcactaata accaaatctt atgtgacata atattttgag tatattttat
    10081 atagagcatt ctcactaaaa aaattacaca tatcgtgagc aatgaactga ttatacttaa
    10141 cattaaaaaa gatgataaca ccttctacac ctccatatca caaaaattat aacattattt
    10201 tgacataaat actacatttg
//
LOCUS       DANJFZ010000094         9814 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.094, whole genome shotgun sequence.
ACCESSION   DANJFZ010000094 DANJFZ010000000
VERSION     DANJFZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.094"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..566)
                     /locus_tag="PWN45_002065"
     CDS             complement(<1..566)
                     /locus_tag="PWN45_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL0623814.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
     gene            644..745
                     /locus_tag="PWN45_002066"
     CDS             644..745
                     /locus_tag="PWN45_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623815.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(830..1513)
                     /locus_tag="PWN45_002067"
     CDS             complement(830..1513)
                     /locus_tag="PWN45_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDL0623816.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1879..2577)
                     /locus_tag="PWN45_002068"
     CDS             complement(1879..2577)
                     /locus_tag="PWN45_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDL0623817.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2957..3655)
                     /locus_tag="PWN45_002069"
     CDS             complement(2957..3655)
                     /locus_tag="PWN45_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDL0623818.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3999..4694)
                     /locus_tag="PWN45_002070"
     CDS             complement(3999..4694)
                     /locus_tag="PWN45_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDL0623819.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(5115..5810)
                     /locus_tag="PWN45_002071"
     CDS             complement(5115..5810)
                     /locus_tag="PWN45_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HDL0623820.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(6256..6960)
                     /locus_tag="PWN45_002072"
     CDS             complement(6256..6960)
                     /locus_tag="PWN45_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDL0623821.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(7324..8250)
                     /locus_tag="PWN45_002073"
     CDS             complement(7324..8250)
                     /locus_tag="PWN45_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDL0623822.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(8359..8496)
                     /locus_tag="PWN45_002074"
     CDS             complement(8359..8496)
                     /locus_tag="PWN45_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623823.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(8615..9673)
                     /locus_tag="PWN45_002075"
     CDS             complement(8615..9673)
                     /locus_tag="PWN45_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDL0623824.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
BASE COUNT         2810 a         1739 c         1288 g         3977 t
ORIGIN      
        1 attaggaatt catatgcatc acctaacata tcaatttcca tgtcactgtg aacgaatggt
       61 aagtcgtcaa gattaaccat gactttagag attaaagcag tacgttcttt gacattgtta
      121 cctagtcgcg ttgaactcaa atccatatcg ctgaacagac cgataaagtc attttcactt
      181 tcttcaccta atgttgatgt ttcaacttta cgaattgccg tcgccaggtg ttcgatatcg
      241 aaatcttgcg tttcaatttc acgaatcatc gcactgaata aatcttctgg ctcaatgaag
      301 taaccgactt ggtcaattaa ttctgctttt aagtcttcac ggtattcttc gtctgcccat
      361 gcttcttgat acgtgatgtc ttcacctgac aaggcatctg catattcttg ttccgctttt
      421 tcagataaga agcgatagaa aatcaagcct aaaatgtaat tacggaattc actcgcatcc
      481 atattccctc ttaaatcatt cgcaatcgac cataattttt tatgtaattc agcttgttgc
      541 tgacgttgtt tttcagtaat agacatgtga ttcctccgcc tttgaataag taatttatct
      601 ctttgtgtaa tagatttatt ataacatttg gttatgttgc gatgtggaga attttgatgt
      661 tggtggtgga aattttacct ttatggagat tgggatagga ttcatgacac acaaaccaaa
      721 acggcttaac gacgatatcg attgatgcat cctttttgta taagctatgc gcaccaattt
      781 acatttcacc gttaagccgc ttcaattact tttaattatt cgtaatgatt tactttaagt
      841 taacttcaat atctttaatt tgtttgcttt ctatgacttc ccccatatat ttaaatttta
      901 atttagatct taaatcaagg ttataaaaac tgccatcttt caagctaatt ttgaccctac
      961 catcttttga gattgtttta taaagtccgt atttttcaat tagatgcttt ctcaacttat
     1021 aatccagttc ttttagtgtt attttttctt ttttaatgta ataagggtaa cctttcacaa
     1081 aagcatcttc acccttttct tttgaaatat ttaatattgg tgcactgaca actccagatg
     1141 ttttattatt ctgtattaca ccaccaacag tataaaatat atcgtgctta tctctttttt
     1201 cttgaacaaa gaaaacatct aacccctcat aactacgctg ttgaaattta cttttatcat
     1261 ttccaggcag taaaacttga atcttaatac ctctaaactt aaaacgtaag ttgtttttac
     1321 catgtttcaa agcactaata tttttcattt ccatagtctt tccagtgtag tatcggtata
     1381 atgcttcctt gtcatgttta tttactgact tttgattttg ttttgcatga cctgaatgaa
     1441 cttctgttgt taaagttcct gttgttaaaa ttcctaaagc taatgtcgct tttgctaatg
     1501 ctgtaaattt catatttatt tgctccaatc ttaatatatt ggattgtttt cattacgtaa
     1561 tttgaatcat acatttttat tataacgtgc atcgtttcat acgataatta acctcgattt
     1621 aaatatactt gaattattct taatgaaaaa caaatatatt ttaatgttca ggttaaatga
     1681 ctttcattat ttcatttagt tgttttaccg gattatcgtt tactacaact gatgatgtta
     1741 ctctgttctg ctctttgaat tacaaaaaca acttaacaac agtaatgtat ttaacacagt
     1801 tccttatata aggacacaaa tgccaattca cattttgtcg ttaagccgct tcttaagcta
     1861 ttatgctatt aactcgaact aattcaaatt cacttcaata tttttaattt gcttactatc
     1921 cattacatca aacatacgtt caaaacttaa tttttcactt aaatcaattt catgcttctt
     1981 ttcgtctttc atattgataa caattctacc tttatcagac gttcctttat acaatctata
     2041 tttttcgatt aagagttttc ttattttaaa gtccagttcc ttcaatgata cttcttcctt
     2101 ttgaataaaa aacaactcgt ttatcgaaaa gccattttta gcatcaactt ttttaacttg
     2161 tagacttgga ttacttacaa atccaaacac tgatctcaca tttttctttg ttataccgcc
     2221 aacgctatat atgccacctt ttatatctat taattcaggt actgcaaaga catcaaggcc
     2281 atgtgttttt tctttatatt tatttttatc gtcaccaagt aaaaatactt taaaagcttt
     2341 atttcgttgt ttaaagttta aaacgttaga gccatagtaa tcttcgcttt taacactaat
     2401 gtttgttggt tcaaaacttt cttcagaata gtattgatgt agcatattga tataatattt
     2461 gcgttgtgtc tcatcaagtt ctactttcgc gtgaccagtt tgactttctg ctgtaaacac
     2521 acctgtagtt aatattccta atgctaatgt tgctttagct atcgttgtta atttcatatt
     2581 tttttgctcc aatcttaatg tattggattg ttattattac gtaatttgaa ctatctgtct
     2641 ttattataag gtgcatcaaa acagactgac attaacgctc atttaaataa acttgaatca
     2701 ttattaatga aaatcaaacg tattttaaca ttcgtattaa ctgattttta atagcaacat
     2761 aaatattttt ttggtgaatc attgttcttt ccgattagtt ttaaatctta agctattggt
     2821 tataaagtgt acaaaaaagc agcttaataa cgtagtcaat gttcgatatg tttttattat
     2881 aaaaacacgt atcaaaatca ttaaacatgg gattattaaa ccgcttctta agctttcatt
     2941 ctatatatat cattgattat ttcaaattca cttcgatgtt tttaatttgt tcactattaa
     3001 tgacatctgc catacgctcg aaatctaatt tatcacttaa atcaatttca tacttatttt
     3061 catctttcat attaataaca attctacctt tatcagctga cccttcatac agtttgtatt
     3121 ttttaatcaa cagttttctt attttaaaat caagttcctt caatgacact tcttcctttt
     3181 gaataaagaa aaattcatca atagaaaaac cgtctttatc gtctattttt ttaactagta
     3241 agttcggcgt tcttagagac tcaaatattg attttacgtt tttctttgtt acaccactaa
     3301 cactaaatat tcttccatct aaatctacta attctggtac cgcaaagaca tctaaaccat
     3361 gtgttttttc tttgtattta ttttcatctt ttcccaataa aaacacttgg aacttttgat
     3421 tttgttggtt aaaaagcaaa acgttagagt cataataacc ttgactttgg ccactaacat
     3481 ttgttaactc ataactaggt cctgaatagt attgatgtaa cttgtttgta tcatataaac
     3541 ggttcatttt ttcatacttt ccttttgcat taaccgattg attttctgtt atcattacac
     3601 ttgttgttaa tattcctaat ataaatatcg ctttagctat cactgtaaat ttcatattca
     3661 tgtgctccaa tcttaatata ttggattgct tttattacgt aatttgaata atacaccttt
     3721 attataaagg gcattgagac agactgacat taacactcgt ttaattaaac ttaaatcatt
     3781 gttaatgaag agctaattaa tattcaatgg gcatcacttt caagagcaat gtaaattttt
     3841 agtatgctca ctcataacct gcgttataaa acgtaaaaaa aagcctaacg acaaggttgt
     3901 tttaaatacg ctccttaatg taaggtgtac gtatctattc aacatttcat cgttaagctg
     3961 cttcaaattt attactttag agtcattgat tacttacttt aaatttgttt caaagtcact
     4021 tcaatcttat taatatcctt actattcaac acatcaccca tgcgctcgaa ttgcaattta
     4081 tcacctaaat caatttcttg cttttctcca tctttcaaat tgatagtgat cttaccgtat
     4141 ttagtcgtac ctttatataa accataattt ttaactaaat gttgtctaat tttgaaatca
     4201 agttctttca gtgaaacttc ttctttatta attgaaaatg agtcaattga tgcatctaaa
     4261 tttccgccat acactttatt aacaaataaa tgtgtattag tttcagaaga tttgttattt
     4321 ttcttagtca caccaccaac agtagataat ctaccgtttg gatcaattaa ttcttttacc
     4381 acaaagacat ctttgccttc aatgccttct ttatatttct ctttatcttt acctaataag
     4441 aataattggt gattttgatt ttcttggtta aagcgtacaa cgttagaacc gttataattt
     4501 tcaaccttac cactaatatt actgaattca aaactttctg atgagtagta tcgatgtaag
     4561 tctttaatat catataaatg ttgtactctc tcttgctttt cttttgcttg aactgcttgg
     4621 ccttctgatg taatcacacc agtagttaat aagcctaatg ccaatgttgc tttagctaac
     4681 gtttttaatt tcacagtact attctcccaa tctatttata aatttgtctt aatatatttt
     4741 tatatgatta attcaattac gtaatacgaa caatctacta tcattatata agtgagatac
     4801 tgcgctatga attaacctct ttttaactat ttttgaacca acgttaagtt agttaatgaa
     4861 atcctaacag tattaattat tttttataac acaatactaa ttttttaact attcacactt
     4921 tccctcaatt taattatgtt ttccacattg tttcatgtca cgaaaaggac aacgcgcgac
     4981 tataagtatc aaccgttttt ccaaaaactt gatcataaac ccgctccttt tttcatcata
     5041 acaaaataaa aaagaagcta agcaatatgt tatcgcttaa cttccctaca attactagtc
     5101 tgcttgtcca aagattattt atattctagc tcaacattaa tttctttgat tttggtacca
     5161 tctatcgtgt cacccatgcg atgcggttgt agtttttttg taagttcgaa agtataaaac
     5221 ttatcatctt ccattttaac tacaatttta ccttttctat tattaacagc accatataat
     5281 ttttcttctt ccatcaattt ttttcttaat ttaaagtcca actcttttaa cgagatttct
     5341 tctttagtaa ttttaaattt actatcttta gacttttccg atgattcatc aatttttctt
     5401 gttactttaa catcgacttc tttactaaat tctttactat ttgtcttact taaaccacca
     5461 attgaatatt gtcttccatt aacagtttcc tcctcgatga ctaaaaatgc atcttgctta
     5521 tcatgctcac cttgagggta tttttcaata tctttaccaa gcaagccaac aaaataatct
     5581 tttcgatcct tccaaacata tactccttta tctgtcactt tctgtctata caaaccatca
     5641 agatttttta actctatact tggcttgcta tagtagtatt ttaacgattt ataattgtgt
     5701 tgaccttgag ttgattctgc cgcttttact gtttgacttt ctgttgttat tacaccagta
     5761 gctaacaatc ccaatactaa tgttgcttta gctaacgttt ttaatttcat agtactattc
     5821 tcccaatcta tttataaatt ttgtcttaat atatttttat atgattattt caattacgta
     5881 atacgaacaa tctactatca ttatataagc gtaatactaa gctatgaatt aacctctttt
     5941 taactatttt tgaaccaatg ttaagctaat taatggaatc ctaacagcgt taatctattt
     6001 tttaaactta acgcagcttt tttaactatt cacactttcc ctcaatttaa ctatgttttc
     6061 cacattgttt catgtcacga aaaggacaac gcgcgactat aagtatcaac tatttccaca
     6121 agttttattg gtgtttttat tattcatcga tacgcttcat tttcatctct ccaacacaaa
     6181 aaagaagcta agcaacttat gttgcctaac tcctctatac tatccatatt ttactattat
     6241 ccatatttca ttgaattatc taatgttggc ttctattttt tcaatatttc taccgtcaat
     6301 gacgtcactc atgcgatttg tttgtaattt tttattaagt tcaaacgtat aatagccgcc
     6361 atctttcatt atcactttta tcttactatc tttaggaaac tttttataca gatcaaaatt
     6421 ttgaattaaa tactgtctca atttaaagtc gagttcttta agtgaaatct cttctttata
     6481 aatgtagtgt actctaccgt acgtagcaat accgtcacct tcatctctct tgatttgaaa
     6541 tcttggtgcg tttatataat cataataagc gtcttgattt ttcttagtga caccaccata
     6601 tgaaaacact gtgccattac ggttttccgc ttctttaaca acaaatatgt ctaatcccgg
     6661 atttttacgt gctttaaatc tttcaatatc tttaccaaat atctgtactc ttgtgaattt
     6721 tctattttta tcaaagataa ggtaatgctt gccacctttg ctataacgat aaccagtaac
     6781 atttttaagt tccttacttg cgccactata gtaatctctt aagtcaaaga tatcttttgt
     6841 cacattttca tattttgctt tatgttcact cgcatttaca gtttgatgca atgacgttat
     6901 tgttcctgtt gctaaaatac ctaatgctaa acttgctttc gcaattgctg tcattttcat
     6961 agttgtatgc tccattcgta attattagat ttgttcgctt acgtctattg aatcatacag
     7021 ctttattata gttagcgtat ttgacctttc acattaaacc atgtttaata atcattgaat
     7081 cattattaag taaattaagg aatctataat gttcgttaaa taaaactgat cccgttgtgc
     7141 ttcacacccg atagataggg atttacagat aaattcaggt ctcttccacg tcatatttgg
     7201 acccatcgaa aattcgggtt ctcaaatcat cgaacataac aaaagaagct aagcaacatg
     7261 taggccgttg tcacttaact tcttgttttt ccgatgacag cttctattta gagaatgtca
     7321 tgattatttt atattcactt caatgttatc aatattagtg ccatctatga catctgccat
     7381 gcgattttct tgtaattttt tgtgcaattc aaacgtgtac tttccaccgt ttttcatttt
     7441 aataacaatt ttacctgaac caacgttacc gtacagatta tttttttcaa taagttgttt
     7501 tctcaattta aaatcaagtt ctttcaagga aatctgttct ttagtaatct tgaattctga
     7561 aacatcatga gagattgtac ctttattatc ttccttagta attcttactc ctgctttgtg
     7621 atcaactttt ttactattac tctttgtgat accaccgaca gaatattttt ccagattgta
     7681 attattttct tctaaaacga caaatacatc gacattccta tgtactcctt caccatattt
     7741 tttatcatct ttaccaacta aagcaatttt atatatgaaa taatctggga caacattcat
     7801 aaatcttatt gtcgtccatt tttttaaaat aataccaatc tcatttttaa attctaaact
     7861 tggtttcgta taatacgctc ttaaatcttt aaatttagga tttatttctg ttggtacttg
     7921 ttttgtggtt ggcgattgtg gtgtgtctga tttagtagat tgcattggtt gtggcgtgtt
     7981 tgttgatgga ggtgttgtca ctttagttga aggcggtgtt gtcgcatttg ctgtttgttg
     8041 cggtgcttct actttagttg agggcggtgt tgtcgcgttt ggttttgatt gcggtgcttc
     8101 tattttagtt gagggcggtg ttgattgtgg tgcttccact ttagtggaag atggtgttgt
     8161 cgcgtttgct gcttgcgttg tcgttgtgat tacacctgtt gttaaaaggc ctagtgctaa
     8221 acttgtttta gcaatcgttg ttattttcat agttgtatgc tccattcgta attattagat
     8281 ttgttcgatt acattcattg aatcatacag ctttattata gatggcgtat tgctccattc
     8341 acattaaacc ttgtttaact atatttgaat catcgttaag taaattaaga aatccataat
     8401 gttcgttaaa taaaaatgat tttgatgtga ttcaacactt ggcacatttg aagtttcgtc
     8461 acttttaaga catagaaatg ccacttttac aaacaaatga atattcgtct ttttacatca
     8521 ttacgcataa taaaagaagc taagcaacat gtaaaccgtt gtcacttaac ttcttgtttt
     8581 tccgatgaca gcttctattt agagaatgtc atgattattt tatattcact tcaatgttat
     8641 caatattagt gcctgccata cgatgctctt gcagtttttt gtgtaattca aacgtatatt
     8701 tcccaccgtt tttcatttta ataacgattg ttcctgaacc catgttaccg taaagattat
     8761 gtttttcaat aagttgtttt ctcaatttaa aatcaagctc tttcaaggaa atctcttcct
     8821 tagtaatcat gtattctgaa acatcgcgtg aaatcatacc ttgattatct tttttagtaa
     8881 tgcttaattc tactttgtga ttaacttttt tactattagt cttcgtgatg ccaccgacag
     8941 aatatttttt caattgatat ttattgtctt ctaaaacgat aaatacatcg atattatcgt
     9001 aaggtccatc tttatatttt ttctcatctt ttccaactaa agctatttta tagatgaacc
     9061 tatttggaat aacattcata aacctaaccg tcgtccatgg tttgagcata aatccaaact
     9121 gcttttcaaa ttcaaaactc ggttttgtat aatacgctct taaatcttca tatttaggag
     9181 tcatatctgt ttgtgcttgt tttatggttg gagattgtgg tgtgtctgat ttagtagatt
     9241 gcattggttg tggcgtgttt gttgatggag gtgttgtcac tttagttttc ggcgttgtgg
     9301 attcggttgt cgtttgtgat tgttcttgtt taggcgctgg cgttgctgat atattaagcg
     9361 ttttctgctc ttcttgttta ggttgtgata ttttttctat tttggaagct gaggtttttt
     9421 cctcattagt atttggtgcc ttttcgagtt taggcgtgcg ttcttgtctt gtgttagctg
     9481 cttgtgttgt cgctgaattt gcacctgctg ttatgtttat cattgctaat cgctctgctt
     9541 taagcgttgg tactttgtca actttagttg attgtatttt ttctgctttg accgattgcg
     9601 tcgttactgt aattgcgcct gttgttaaaa gccctagtgc taaactggtt ttagcaattg
     9661 ttctcatttt cataattgta tgctccaatc tatattatat tcgattgtct ttttacgtaa
     9721 tttgaatcat acaacatcat tatagatggc gttctaagat aatcacatta aacccctttt
     9781 aacaattatt gaagtattat taagtaattt aagc
//
LOCUS       DANJFZ010000095         9715 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.095, whole genome shotgun sequence.
ACCESSION   DANJFZ010000095 DANJFZ010000000
VERSION     DANJFZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.095"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(255..1052)
                     /locus_tag="PWN45_002076"
     CDS             complement(255..1052)
                     /locus_tag="PWN45_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0623825.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            1413..2057
                     /locus_tag="PWN45_002077"
     CDS             1413..2057
                     /locus_tag="PWN45_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL0623826.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            2754..4903
                     /locus_tag="PWN45_002078"
                     /pseudo
     CDS             2754..4903
                     /locus_tag="PWN45_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            5049..5408
                     /gene="sarX"
                     /locus_tag="PWN45_002079"
     CDS             5049..5408
                     /gene="sarX"
                     /locus_tag="PWN45_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDL0623827.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            5598..6314
                     /locus_tag="PWN45_002080"
     CDS             5598..6314
                     /locus_tag="PWN45_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0623828.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            6314..6787
                     /locus_tag="PWN45_002081"
     CDS             6314..6787
                     /locus_tag="PWN45_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL0623829.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            6879..6995
                     /locus_tag="PWN45_002082"
     CDS             6879..6995
                     /locus_tag="PWN45_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623830.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            7140..7784
                     /locus_tag="PWN45_002083"
     CDS             7140..7784
                     /locus_tag="PWN45_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL0623831.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            7900..8766
                     /locus_tag="PWN45_002084"
     CDS             7900..8766
                     /locus_tag="PWN45_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0623832.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            9036..>9715
                     /locus_tag="PWN45_002085"
     CDS             9036..>9715
                     /locus_tag="PWN45_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623833.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIA"
BASE COUNT         3580 a         1220 c         1633 g         3282 t
ORIGIN      
        1 ggtataacaa attaaatcat tatatagtca acttagttgc attattttga tttttaatat
       61 attatgcatt taatttaatc aaataggtag gttgttaatt tttacctaac taccattcat
      121 acaaaatata aatatgtatg atagggaagg gtgagataat tttttgtacc aaaatgaaaa
      181 tttttttcaa aaataaaaaa ctagcaatct cacatgatgt gaattgctag tatatatcag
      241 tacaatttat ttaattaatg gatgaatgca tagctagaaa cttctgaagc tggaattgta
      301 cggtagttca tattgtatgg accatatgtg taattcattt cagaaatcaa gatactacca
      361 tcaccattga cacgttcaac ataagcaaca tgaccatatg gaccaggtgt gctttgcata
      421 attgaaccaa ctgatggtgt gttgtttact tggtaaccat cattagctgc gttaccagcc
      481 caatacttag cgtctgacca atatgtgcta attggactac cagcttgagc acgacggtca
      541 aatacgtacc atgtacattg accagcagtg tataaatttt ggtgattaaa agatgatgca
      601 ttgccattgc tacctgttgt agctgttggt gttgtaccac ctgatccacc attaggaatt
      661 tgtaatgttt ggttaggcat aattaaataa ccacgtaagt tattggctgc cattaattga
      721 tcaactgaaa caccatatct gctagcaatg atatttaatg attcaccagc ttgtacagta
      781 tgagatgatg ctgaaccagc ttgtggagaa gtgtttgacg tattttgtgc atcacttcca
      841 cctactgaga taacttgacc agggaatacc aagttgttat ctaattggtt attttgttta
      901 atactctcta ctgaagtgtt gtatttttga gcaatactcc ataatgattc accagattgt
      961 actgtatgtt gtgtagaagc ttgtgcatca tgatgcgtta aaaatgcagc tgcaccagat
     1021 gttgctgtta ttgcaaatgc taattttttc aaagggactc ctccttaaaa ttatgtttta
     1081 ctacttttaa aaattattaa gacaatttat atagtaaatt aagttttcgt tttatattac
     1141 gatttccata ttatcaaaaa taagtagctt tgtgtggttt gttattatct tgtaatatta
     1201 ttaacatttt aggatatcgg ttcatatttt tataaatgct gatttgatag tgtataaaag
     1261 cgttttagtg attttaacta attataaaaa attatatttt gtaacattgt aaaatgtaaa
     1321 attactatac ttgttaaagg ttatttcttt tgatgaagca aaatttactg taaaatttga
     1381 cgcataaaac gacggtagaa ggaggaatta ttttgtcgca aaatacaaat cattcatatt
     1441 atcatcaaaa ccagcatgct caatcaataa gtaaagtgtg gctttatttt atgtattatt
     1501 ggattatatt tggcatagga tgctatctag gtcagttttt accattaagt tggcgacaac
     1561 ccttgtcatt tggattactg attattattt tagcaacact tgtttttgaa agagcgagac
     1621 ggttcggttt aattatttca catatttacg ctgtagtgat tggcttattg tcatacgcaa
     1681 cgtttaccac gtatttacaa aatttaggac cagatatttt ctataaaaat atcgcattag
     1741 caatttttgc atttatagca tttggtatta ttggttattt cttcgttgga gatgcatcga
     1801 gtataggcaa atatttattc gttacattaa taacattaat tattgcgagt ctaattggta
     1861 tttttcttca aaatcctatt ttttacacta ttattaccgt cgttagtctg ttgttatttc
     1921 tactttatac tttgtatgat tttaatcgtt taaaaagagg tgactattca ccaagagaaa
     1981 tgggatttaa tctatttatt aatttgttga atattattaa ggatatactt taccttgcta
     2041 atatgttcag aagataaaca gttaaattaa taaagaaatt tgtataaagt tttggaataa
     2101 tcaataatga atatcaacct taaactggag cgattgtcat aagttagaaa cttagactga
     2161 gattttactg ataaagcaat aaaaatatat taccttaatt gtaaagatat tattaatcat
     2221 tgaaatgatt aactgctata gttctaaagt aaacaaatct tagaagataa actttaaaca
     2281 ggtgtacttg cctttctaat tacactttta ttagaaaagt aaagtatagc tacgtattaa
     2341 ttttaaaatc gaaacattaa taggaagaag tatagataat ataaacaatt taatgttagt
     2401 ttgagctcat ctcaaaagta gaatgtgatt taaataagtg tttattttta aattgaaaat
     2461 gaatatataa ctaacaaaag ataatagtaa tacgttgtag atgtggtcat ttaaaattat
     2521 tttgaaaata ctaaaaagta gtgtgttttc aattggatag tttaattaaa ctgactacat
     2581 ctatttttgt aatatttaaa tgattgatat ataagtttaa aaattaacca ttatatagtt
     2641 ttatttattt ttgatttggt attcgtattg tgttaaattt acgtttaatt aaataagtta
     2701 aatattatag ggaatgataa aagtggtact taaatataga aagaggtaaa gttatggcaa
     2761 aatcatgctt gcatatactt actaataatg aatatgcgac aacgcgttgc caagatggca
     2821 tagtcttatt ttggccaatt gacggggaaa tcgaactaca aaaatttcgt aaaagtaaaa
     2881 taattgaaga tgatatatat attattaatc atctggatgt atttagtatt aagaataata
     2941 aaaaaacgat catgttgtat ttgagtagcg attggtttgc ggaattaggc tttactttct
     3001 ttaattacca ctatacagca aagttgatta aatcatccta taatttgaaa tgtctactat
     3061 taaaattgac atatcgatac cttgataatc agcctcttaa tgacgctgat attagaaaat
     3121 tacaggatat tattaaaatc attgcaaaag aagcaagtat ggataaaaag attgcacaaa
     3181 atcaatatcg atatgcgtat tatggtgatt tgcgtgatga gctcgaatat atttatcaaa
     3241 atgtaaatca acgattgaca ttaaaaagtg tcgctgataa attatttgtc tcaaagtcaa
     3301 atttgtcatc acaattccac ttacttatgg gcatgggttt taaaaaatat attgatactt
     3361 tgaaaattgg taaatcgatt gaaattctac ttactactga tagtactatt agcaacataa
     3421 gtgaacattt aggttttagt agtagctcca cttactctaa aatgtttaaa agttatatgg
     3481 atataacacc gaatgaatat cgtaatttat caaaatataa taaatgttta atgctaaagc
     3541 cagaaccact agtaggcaaa atggtgcaag aagtaaaaga aatcatattg aattatattg
     3601 aacattataa aaaccaccta actgatgtta tacatattga tgaagacaaa tttgaaacac
     3661 ctaaattgtt tcaaacggtt attcaaataa atacttatac agaaatgaaa ttagttttct
     3721 tagaaggaat ctttaaaacc ttattgaata agaacagtca agttgtcttt ttcatcatgc
     3781 catcgattct aaaaagtaaa aataccatgt ccgaagaaga aaaattcaca atcattaaaa
     3841 caataattga aagtgatcta aagatagcat ttaatataaa taatattgaa acaacttatt
     3901 ttgttgaaga agcttttatg agtgttttca gacaaatatc tccaaacgaa ttaagtaatc
     3961 ataataatta cgaagtgcat tttgtttttg atttatcatt gatggaaatt agaacaattt
     4021 atcgaatgat attaaaatta cataacatca tgttgaatgt gaaattagga ttgaacatta
     4081 cctgtttatt tgaaaaacct tcagttttta aatcactagt atcacaaata aagcgactta
     4141 aattcgattc gttaataata gataatgcaa atttaagtag cccttatttg atgggggaaa
     4201 gtgatgagtt actattgaaa aatattttgc attttaaaaa tttaaaacaa gtaattaatg
     4261 aattggatat tgaacaagaa aagctgattt ttctaaatgt tgaaaatcat aaactgctta
     4321 ataataaaga acgagatcta agtaatagtg ctccattaat ttataagaca ttaagtgcgc
     4381 tgtatcacaa ctttgatggc tttggattaa acatttttga caatcatcaa gcatttaatg
     4441 cgatgcatct atatgacaaa aatggattta aaacaacact aggtcttata ttagaaaagt
     4501 ttatcgaata tgtctcgaaa ccaaaatacg aaaacagtta ttattctatt tttgatatag
     4561 agaattatta ttgtcttgtt atttatgatt ggcgagtgat agagagcgag acaattatga
     4621 gtaattttga ggatagtcaa gtttatataa attttaaaaa caatgtttta aacgataaat
     4681 atctaattgt aatagaaaca ttggacaaaa tagtggcaac attaatcatt tgatttctaa
     4741 agaattaaga gataaatatg aatggaaccc tagtttacta tctaaaattg acaactacct
     4801 taaaccagca atagagatta aagagcataa ttttagtgat aattccttga atattaacgt
     4861 tacttttaat gcgttataca taattaaaat aggaaaaaaa taacaccttg atatgtattg
     4921 caaaatttaa tttgcatttg ttggtaattt tgacaacgta ttaaaaacaa atgagaaaga
     4981 caatggctga aaaatttaag ataataatga cagaagcatt gtctttatat atttgggggt
     5041 gcaacatttt gaatactgag aaattagaaa cattgcttgg cttctataaa caatataaag
     5101 cattatctga atatattgat aaaaaatata agttgtcgct aaatgattta gcagtcttag
     5161 atttaacgat gaagcattgc aaagatgaaa aagtacttat gcaatcattt ttaaaaactg
     5221 caatggatga gctagattta agtaggacaa aattattagt ttctataaga agactaattg
     5281 aaaaagaaag acttagtaaa gttagatcat ctaaagatga gcgtaaaatt tatatttatt
     5341 taaataatga tgatatatct aaatttaatg ctttatttga agatgtagaa caatttttaa
     5401 atatttaatt gaaattgagt gtcgaaagca tagaatttgc ttatcggcac atttttaatt
     5461 tatacatatt ttaaaactaa gtaacagttt gaagaaatcg tagttcaata atgttaattg
     5521 tgaaaatgta tataaacata aaaaaatcat gtataatata tgttgttaat taaacagttc
     5581 gaaagcgaga tgacattatg ggacgtaaat ggaataacat taaagaaaaa aaggcccaaa
     5641 aagataaaaa cacaagtaga atatatgcga aatttggtaa ggagatttat gttgcagcaa
     5701 aatctggtga acccaatcca gaatctaacc aagctttaag gttggtgctt gaacgcgcta
     5761 agacatattc agtgccgaat catattattg aaaaagcaat agataaagct aagggtgctg
     5821 gagacgaaaa ctttgatcac ctaagatatg aaggatttgg cccaagcgga tcaatgctaa
     5881 ttgttgatgc gttaacaaat aatgtaaatc gtactgcctc tgatgtgcga gctgcttttg
     5941 gtaaaaacgg cggtaatatg ggtgtatctg gatcagttgc ttatatgttt gatcatgtgg
     6001 caacatttgg tattgaagga aagtctgttg acgaaatact tgaaacatta atggaacaag
     6061 atgtagatgt aaatgatgtg attgacgata atggattgac aatagtctat gctgaaccag
     6121 atcaatttgc agtcgttcaa gatgcgcttc gtgcagcagg tgttgaagaa tttaaagttg
     6181 ctgaatttga aatgttacct caaacagata ttgaactttc tgaagcggac caagtaacat
     6241 ttgaaaaatt aatcgatgca ttagaagatt tagaagatgt acaaaacgta ttccataatg
     6301 tggatttgaa ataatgaaat cagcagaaca atggattgat gaattgcaac ttgaatcaca
     6361 tcctgaaggt ggtttctata gagagacaat tcgagaagta ttgaaagatg gacgcagagc
     6421 gccgtttagt agtatttatt ttttacttac agatgacaat atttcgcatt ttcatcgaat
     6481 tgatgctgat gaagtatggt actatcatgc tggcgattct ctaacaattc atatgataaa
     6541 tccggatggg gaatatacga ctgcaacatt gggtactgat atccaaaatg gagatgtatt
     6601 gcaatatgta gtgcctaaag gaacaatttt tgcttcttca atcgaaatat caaatacttt
     6661 tagtttagta ggttgtatgt gtcaaccggc atttgagttt aagcagtttg aattgtttaa
     6721 gcaatctgaa ttaattacgc aatatccgca tcttaaatca gtaatcgaaa aatatgcttt
     6781 aaaataaaag tgatcaatga agtggtttga aggttgttaa taaacctttg agtcacttca
     6841 tttttatatg tattcttgat tgaatcagaa tagatttgat gcttcagctg tttttaatga
     6901 aaatagcatt aaatgatttt gaaaacgata agagtgtgtt atttatattt ttgaaaaatc
     6961 acttttatga agaaatgtgt gtactatcta attaatattg tctattttaa gtaaattgaa
     7021 gcgagttgta gtaaaataat aataaatatt ttcatatgat taacaaaaac tataaaactg
     7081 tatcatgaca tgacatcatt tatgtagttt tatataataa tgacaaggag ttggtgactg
     7141 tgaaagtaaa gtatatagat aaacgtcact ggcgtcgcct aattgatagg gaatacacag
     7201 aggtaaaagt taataataat aggtttaagg gtattatagg cttagtcacg atgaaaaagg
     7261 ttcgtgatcc tttagaggtg acggtagttg gacaaaatat cattgtcgca gatgacaatt
     7321 ataaatggtt gcaaatacta cctgaaaaga aacgttatag tataactgta atgtttgata
     7381 ataaaggcaa tccattagaa tattattttg atataaatat caaaaatata acgcaaaaag
     7441 gtaatgcgcg tacagtagat ttatgtttag atgttttagc attaccaagt ggtgaatatg
     7501 agttggtaga tgaagatgat ttaatgtttg cattagaaag tgagcaaatt acaaaaaagc
     7561 aatttcatga agcatatatg attgcacatc aaattatggc agagttagaa aatgatttta
     7621 aaggattcca aaagaaaatc atgtactgct ttaataaaat taatgcaaag gctcaaaaaa
     7681 atcatcaaaa gccacaaaat aaaactaata ttgaaaaaag caaacaaata aagcctaagc
     7741 aatataatca aactaaaaat caccaacaac aaaagaaaaa ctaagtaatt caagctgcag
     7801 ccataccaat aaaattggtg aaattattcg ttagtaatga actcatgaat gcttgtagcc
     7861 atgaaagttc aataattgaa taatttatgg ggggaaatta tgaagattga agactatcgt
     7921 ttactaataa cattagacga aacgaaaacg ttacgtaaag cggctgaaat tttatatata
     7981 tctcaacctg ctgttacaca aagactaaaa gctattgaaa atgcttttgg agtagatatt
     8041 tttatcagaa caaaaaaaca attgattaca acaactgaag gaacaatgat tattgagcat
     8101 gctcgtgaca tgttgaaaag agagcgatta ttttttgaca aaatgcaggc acatattggt
     8161 gaagtgaatg gaacaatatc aatcgggtgt tcttctttga ttggacaaac cttacttcct
     8221 gaagttttga gcctatataa tgcccaattt cctaatgttg aaatacaagt gcaagttggt
     8281 tcaactgaac aaattaaagc aaatcataga gattatcatg ttatgataac tcgtggaaat
     8341 aaggtaatga atttagctaa cacacattta tttaatgatg atcattattt tatttttcca
     8401 aaaaatagac gagatgatgt tacaaagtta ccatttatag agtttcaagc tgatccgatt
     8461 tatataaatc aaataaaaga atggtataac gataatttag aacaagatta ccatgcaact
     8521 attacagtgg atcaagtagc aacttgcaaa gaaatgttga ttagtggtgt aggtgttaca
     8581 atcttgccag aaattatgat gaaaaatatc agcaaagaac aatttgagtt tgaaaaagta
     8641 gaaattgata atgaaccgct gattcgttcg acatttatga gttatgatcc gagcatgttg
     8701 caattgccac aagttgattc ttttgtaaat ctcatggcga gctttgttga acaaccaaag
     8761 gcgtagtttt agactaattt aaggtttgta tttaatttta aactattcgg ttaaattgaa
     8821 cgtagttggt tgctaatgca ccaacagcaa aagagcccct aattaataaa ttaaaagggg
     8881 acaaaggaat acagttggtt gctaatgcac caactgcata agagccccta attaataaat
     8941 taaaagggga caaaggaata cagttggatg ctaacgcacc aactgcataa gagcccctaa
     9001 ttaataaatt aaaaggggct ctaagaagcg gggttatgga tatgtttgca gcgttattac
     9061 aaataaagaa ttataaactc tttgttgcta atatgtttct actaggtatg ggtattgcgg
     9121 ttacggtccc atatcttgtt ctttttgcaa ctaaagattt aggtatgaca acaaatcagt
     9181 atggattact tctagcatct gcagcgatta gccagtttac agtaaattca attattgcta
     9241 gattttcgga tacgcatcac tttaatagaa aaattattat tattctcgca ttattaatgg
     9301 gtgcgcttgg tttttcaata tacttttttg tagatacaat ctggttattc atattactat
     9361 atgcgatttt ccaaggatta tttgcaccag caatgcccca actttacgca tctgctagag
     9421 aatctatcaa tgtttcaagc tctaaagata gagctcaatt tgccaacaca gtattacgtt
     9481 caatgttctc attgggcttt ttatttggtc catttattgg tgcccaatta atcggattaa
     9541 aaggctatgc tggattgttt ggtggaacaa taagtatcat tttatttact ttagtacttc
     9601 aagtgttttt ctataaggat ttaaacatta aacaccctat tagtacgcaa caacatgttg
     9661 aaaaaattgc tcctaatatg tttaaagaca aaacgctttt attaccattt attgc
//
LOCUS       DANJFZ010000096         9674 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.096, whole genome shotgun sequence.
ACCESSION   DANJFZ010000096 DANJFZ010000000
VERSION     DANJFZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.096"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            191..406
                     /locus_tag="PWN45_002086"
     CDS             191..406
                     /locus_tag="PWN45_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623834.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            431..694
                     /locus_tag="PWN45_002087"
     CDS             431..694
                     /locus_tag="PWN45_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0623835.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            706..867
                     /locus_tag="PWN45_002088"
                     /pseudo
     CDS             706..867
                     /locus_tag="PWN45_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            960..1277
                     /locus_tag="PWN45_002089"
     CDS             960..1277
                     /locus_tag="PWN45_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623836.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            1270..1572
                     /locus_tag="PWN45_002090"
     CDS             1270..1572
                     /locus_tag="PWN45_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDL0623837.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            1577..1837
                     /locus_tag="PWN45_002091"
     CDS             1577..1837
                     /locus_tag="PWN45_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDL0623838.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            1851..2213
                     /locus_tag="PWN45_002092"
     CDS             1851..2213
                     /locus_tag="PWN45_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623839.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            2210..3376
                     /locus_tag="PWN45_002093"
     CDS             2210..3376
                     /locus_tag="PWN45_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDL0623840.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            3402..3959
                     /locus_tag="PWN45_002094"
     CDS             3402..3959
                     /locus_tag="PWN45_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDL0623841.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            4027..5979
                     /locus_tag="PWN45_002095"
     CDS             4027..5979
                     /locus_tag="PWN45_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDL0623842.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5992..6177
                     /locus_tag="PWN45_002096"
     CDS             5992..6177
                     /locus_tag="PWN45_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDL0623843.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            6177..6578
                     /locus_tag="PWN45_002097"
     CDS             6177..6578
                     /locus_tag="PWN45_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDL0623844.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            6578..6835
                     /locus_tag="PWN45_002098"
     CDS             6578..6835
                     /locus_tag="PWN45_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDL0623845.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            6838..7038
                     /locus_tag="PWN45_002099"
     CDS             6838..7038
                     /locus_tag="PWN45_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623846.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            7053..7295
                     /locus_tag="PWN45_002100"
     CDS             7053..7295
                     /locus_tag="PWN45_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623847.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7310..7558
                     /locus_tag="PWN45_002101"
     CDS             7310..7558
                     /locus_tag="PWN45_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDL0623848.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            7551..8087
                     /locus_tag="PWN45_002102"
     CDS             7551..8087
                     /locus_tag="PWN45_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDL0623849.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            8124..8330
                     /locus_tag="PWN45_002103"
     CDS             8124..8330
                     /locus_tag="PWN45_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDL0623850.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            8327..8530
                     /locus_tag="PWN45_002104"
     CDS             8327..8530
                     /locus_tag="PWN45_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623851.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            8523..8759
                     /locus_tag="PWN45_002105"
     CDS             8523..8759
                     /locus_tag="PWN45_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623852.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            8752..9138
                     /locus_tag="PWN45_002106"
     CDS             8752..9138
                     /locus_tag="PWN45_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623853.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            9135..9287
                     /locus_tag="PWN45_002107"
     CDS             9135..9287
                     /locus_tag="PWN45_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0623854.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            9355..9555
                     /locus_tag="PWN45_002108"
     CDS             9355..9555
                     /locus_tag="PWN45_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDL0623855.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
BASE COUNT         3756 a         1298 c         1944 g         2676 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc ttttatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgtatactta tcacctcctt aggttgataa caacattata cacgaaaggt
      181 ggaacaacaa atgaacaaaa aatcagaagg gttagacata atcataccaa ggattttcag
      241 aagagatcat gcgtcagtag aatctttaac agaaaatgaa cgtcgactaa gaaaggaaat
      301 attaggaagt attaaaaaag gtgattacag ctacttagaa ataaacaaag ttttctatgc
      361 attagataga gaacttcaat acagagcgaa taataacaaa ctttaacatt tatcgaaagg
      421 agtgatagag atgccaaaaa tcatagtacc accaacacca gaaaacacat atagaggcga
      481 agaaaaattt gtgaaaaagt tatacgcaac acctacacaa atccatcaat tgtttggagt
      541 atgtagaagt acagtataca actggttgaa atattaccgc aaagataatt taggtgtaga
      601 aaatttatac attgattatt caccaacagg cactctgatt aatatttcta aattggaaga
      661 gtatttgatc agaaagcata aaaaatggta ttaggaggat attaaatgag cgacacatat
      721 aaaagctaat taatagcagt actgtgcttc acagtcttag cgattgtgct tatgccattg
      781 ctgtacttca ctacagcatg gtcaattgcg ggattcgcaa gtatcgcaac attcatatac
      841 tacaaagaat acttttatga agaataaaaa aactgctact tgcgtcaaca agtaacagtg
      901 acaaacattt atcaaaatat acaacttaat taaatcaaaa tatacggagg tagtcaacta
      961 tggctgaaaa tattaaaact gaacaacatt attacactaa agatttctca ggatacagaa
     1021 atgaagaaga taactttgta gcaaatcaag aattgacagt aacaatcaca ttgaacgagt
     1081 acagaaaact tattgaaata aaggctgtta aagataaaga agaagatact tacagaggta
     1141 agtattttgc ggaagaaaga aaaaacgaaa aattggaaaa agaaaatata aaactaaaaa
     1201 acaaaattta tgaattacaa aacgaagaag ataacgagga ggacgaagaa aacgaggagg
     1261 aagtcaacaa tgactaaaaa atataaagac atgactcagg aagaagtaaa agacttatta
     1321 tctgaaaaaa gcggagaatt gtatgaatta gcgaaagaaa ttaagggaga aagtaaattt
     1381 gatattttgc ttttctcatc aataggagtt atcgacggag attatttagc aggttcaagt
     1441 tctgtgattg gtcatacttt cgatcttgct tccttattgg atagcactaa gagttataaa
     1501 gacattgtca atgttctcca aatgtgtaaa tcacaaaaat ttcacggtat tgatgataac
     1561 aaggagggct aaaacaatgt attacaaatt tggtgagata aaaaataaaa ttatcagctt
     1621 taacgggttt gaatttaaag tgtctgcgat gaaaaaacat gacggtatca gtatacaaat
     1681 caaggatatg aataatattc cacttaaatc atttcatgtt gtagatttaa gcgaactata
     1741 ttttgcgacg gatgcaatgc gtgacgttat aaacgaatgg attgaagaga acacagatga
     1801 acaggacaga ctaattaact tagtcatgaa atggtaggag gatttaatca atggcaatat
     1861 tagaagatat ttttgaagaa ttaaaactat taaataacaa tttacgtgtg ttaaacactg
     1921 aactatcaac agtagattca tcaattgtac aagagaaagt taaagaagca ccaatgccaa
     1981 aagaagaaac agctcaactg gaatcaattg aagaagttaa ggaaacttct gctgatttaa
     2041 ctaaagatta tgttttatca gtaggaaaag agttccttaa aaaagcagat acttctgata
     2101 agaaagaatt tagaaataaa cttaacgaac ttggtgcgga taagctatct actatcaaag
     2161 aagagcatta tgaaaaaatt gttgatttta tgaatgcgag aataaatgca tgaagctaga
     2221 tcactcaaat agagctcatg caaagcttag tgcaagtgga gcaaaacaat ggctaaactg
     2281 cccaccgagt attaaggcaa gtgaaggtat tgcagataaa agtacagttt ttgccgaaga
     2341 aggtacattc gcccatgagt taagtgagtt atatttcagc cttaaatatg aaggcctaac
     2401 acagtttgag tttaataagg cttttcaaaa ttataagaga aatcaatatt acagcgaaga
     2461 attgcgtgaa tatgtggaag agtatgtagc taatgtagaa gaaaaatata acgaagcttt
     2521 gagtagggat aatgatgtaa tagctttatt tgaaacaaaa ttagatttag gtaaatacgt
     2581 ccctgaatct tttggaactg gtgatgtcat tatattttca ggtggtgtac ttgaaattat
     2641 tgaccttaaa tatggtaaag gcattgaagt ttcagctata gataatcctc aacttagatt
     2701 atatggcttg ggcgcatatg aactgcttag tttaatgtat gacattcata caattcgcat
     2761 gactatcata caacctcgaa tagataactt ttctactgaa gagttaccaa tatcaagatt
     2821 acttcaatgg ggagccgatt ttgttaaacc attagccaga cttgcttata acggtggagg
     2881 agagtttaaa gcaggtagtc attgtagatt ctgtaagata aatcattcat gtagaacacg
     2941 tgcagaatac atgcaaaatg tgcctcaaaa gccaccacat ttgttaagtg atgaagagat
     3001 tgcagaactt ttatataaac tgcctgatat caaaaaatgg gctgatgaag tagaacaata
     3061 tgcactaaat caagcgaaag aaaatgataa aaactatcct ggttggaagc ttgtagaagg
     3121 tcgttcacga agaatgataa ctgatacaaa agcaatgctt gaaaagttag ttgaagcggg
     3181 ttataaacct gaagatatta cagaaaccaa gttacttagc attacgaatt tagaaaaatt
     3241 aattggtaaa aaagcatttt ctaaaattac agagggtttt atagaaaagc cgcaaggtaa
     3301 attaacactt gctaccgagt cggataaacg accagctata aagcaatctg ctgaagatga
     3361 ttttgacaaa ctataaaaat ttaaaaggac ggtatataaa catgaaagca aaagtattaa
     3421 ataaaactaa agtgattaca ggaaaagtaa gagcatcata tgcacatatt tttgaacctc
     3481 acagtatgca agaagggcaa gaatcaaagt attcaatcag tttaatcatt cctaaatcag
     3541 atacaagtac gataaaagcc attgaacaag ctatagaagc tgctaaagaa gaaggaaaag
     3601 ttagtaagtt tggaggcaaa gttcctgcaa atctgaaact tccattacgt gatggagata
     3661 ctgaaagaga agatgatgtc aattatcaag acgcttattt tattaacgca tcaagcaaac
     3721 aagcacctgg tattattgac caaaacaaaa ttagattaac ggattctgga actgttgtaa
     3781 gtggtgatta tattagagct tcaattaatc tatttccttt caacacaaat ggtaataagg
     3841 gtatcgcagt tggattgaac aacattcaac ttgtagaaaa aggcgaacct cttggcggtg
     3901 caagtgcagc agaagatgat ttcgatgaat tagacactga tgatgaggat ttcttataag
     3961 tcaataggtg gggttttagc cccactttaa ttttaaagaa attgaggtgt caagaatttg
     4021 agatttatga atatagatat tgaaacatat agcagtaatg atatttcgaa atgtggtgcc
     4081 tataaataca cagaagctga agatttcgaa attttaatta tagcttattc aatagatggt
     4141 ggagcgatta gtgcgattga catgactaaa gtagataatg agcctttcca cgctgatttt
     4201 gagacgttta aaattgctct ttttgatcct gctgtaaaaa agtatgcatt caatgctaat
     4261 ttcgaaagaa cttgtcttgc taaacatttt aataaacaga tgccacctga agaatggatt
     4321 tgcacaatgg ttaattcaat gcgtattggc ttacctgctt cgcttgataa agttggagaa
     4381 gttttaagac tacaaaacca aaaagataaa gcaggtaaaa atttaattcg ttatttctct
     4441 ataccttgta aaccaacaaa agttaatgga ggaagaacaa gaaacttgcc tgaacatgat
     4501 cttgaaaaat ggcaacaatt tatagattac tgtattcgag atgtagaagt agaaatgacg
     4561 attgctcata aaattaaaga ctttccagta actgcaattg aacaagcata ttgggttttt
     4621 gaccaacata taaacgacag aggtattaag ctttctaaat cattgatgtt aggagctaat
     4681 gtgctcgata agcagagtaa agaagaattg cttaatcaag ctaaacatat aacaggttta
     4741 gaaaatccta atagtcctac acaattattg gcttggttaa aggatgacca aggattagat
     4801 atacctaatt tacaaaagaa aacggttcag gagtacttaa aagaagcaac aggaaaagct
     4861 aaaaaaatgc tagaaattag attgcaaatg tctaaaacca gtgtgaaaaa atacaacaaa
     4921 atgcatgaca tgatgtgcag tgatgaacgg gtaagaggtc tgtttcaatt ttacggtgcc
     4981 ggtactggaa gatgggcagg tagaggtgta caacttcaga atttaacaaa gcattatatt
     5041 tcagatactg aattagaaat agcaagagat cttattaaag aacaacgttt tgacgattta
     5101 gatttattac tcaatgttca tcctcaagac ttattaagtc aattagttag gacgacattt
     5161 actgctgaag aaggtaatga actagcagta agtgattttt ctgcaataga ggcaagagtc
     5221 atagcatggt atgcaaaaga acaatggcgt ttagatgtgt tcaacacaca cggaaagata
     5281 tatgaagcat cggcttctca aatgtttaat gtcccggtag aaagcataac taaaggcgac
     5341 cctctcagac aaaagggaaa agtgtccgaa ttagctttag gttatcaagg tggcgctgga
     5401 gctttaaaag cgatgggcgc attggaaatg ggcattgaag aaaatgaatt acaaggttta
     5461 gttgatagtt ggcgtaacgc aaatcctaac atagttaatt tttggaaggc ttgccaagag
     5521 gctgcaatta atactgtgaa atcccgaaag acgcatcata cgcatggagt tagattttat
     5581 atgaaaaaag gttttctaat gattgaactg cctagtggaa gagctttagc ttatccaaaa
     5641 gcttcagttg gtgaaaatag ttggggtagt caagttgttg aatttatggg cttagatctt
     5701 aaccgtaaat ggtcaaagtt aaaaacgtat ggtgggaagt tagtcgagaa tattgttcaa
     5761 gcaactgcaa gggatttact tgcgatttct atagctaggc ttgaagcatc aggttttaaa
     5821 atagttggac atgtccatga tgaagtaatt gtagaaatac ctagaggttc aaatggactt
     5881 aaggaaatcg aaactatcat gaataagcct gtcgattggg caaaaggatt gaatttgaat
     5941 agtgacggat ttacttctcc gttttatatg aaggattagg agtgtgattg aatgcaacat
     6001 caagcttata tcaatgcttc tgttgacatt agaattccta cagaagtcga aagtgttaat
     6061 tacaatcaga ttgataaaga aaaagagaat ttggcggact atttatttaa taatccaggt
     6121 gaactattaa aatataacgt tataaatatc aaggttttag atttagaggt ggaatgatgg
     6181 ctagaagaaa agttataaga gtgcgtatca aaggaaaact aatgacattg agagaagttt
     6241 cagaaaaata tcatatatct ccagaacttc ttagatacag atacaaacat aaaatgcgcg
     6301 gcgatgaatt attgtgtgga agaaaagact caaaatctaa agatgaagtt gaatatatga
     6361 agagtcaaat aaaagatgaa gaaaaagaga gagaaaaaat cagaaaaaaa gcgattttga
     6421 acctatacca acgaaatgtg agagcggaat atgaagaaga aagaaagaga agattgagac
     6481 catggcttta tgatggaacg cctcaaaaac attcacgtga tccgtactgg ttcgatgtca
     6541 cttataacca aatgttcaag aaatggagtg aagcataatg agcataatca gtaacagaaa
     6601 agtagatatg aatgaaacac aagacaatgt taaacaacct gcgcattaca catacggcga
     6661 cattgaaatt atagatttca tcgaacaagt tacggcacag tatccaccac aattagcatt
     6721 cgcaataggt aatgcaatca aatacttgtc tagagcaccg ttaaagaatg gtcatgagga
     6781 tttagcaaag gcgaagtttt acgtcgatag agtgtttgac ttgtgggagg ggtaacgatg
     6841 gcaacgcaaa aacaagttga ttacgtaatg tcattacagg agcaactgga attagaagac
     6901 tgcgaaaaat atacagacga acaagttaaa gcaatgagtc ataaagaagt tagcaatgtg
     6961 attgagaact ataagacaag cataaggaat gaagaactat attacgaatg catgtcgttt
     7021 ggactgccta attgttaaaa ggagtgacga ccatgacaga tagcgcacgt aaagaacgct
     7081 taaaccaatt tttcggctct aaaagatatc tgtatcagga taacgaacga gtggcacata
     7141 ctcatgtagt aaatggcacg tattactttc atgggcatat cgtgccaggt tggcaaagcg
     7201 ttaaaaagac atttgatact gctgaagagc tcgaaatata tataaagcaa catggtttgg
     7261 aatacgagga acagaagcaa ctaactttat tttagaggag atggaaatga tgaataaccg
     7321 cgaacaaatt gaacaatcaa ttatcagtgc tagtgcgtat aacggcaatg acacagaggg
     7381 attactaaaa gagattgaag acgtgtataa gaaagcgcaa gcgtttgatg aaatacttga
     7441 aggtttacct aatgctatgc aagatgcact caaagaagat attggtcttg atgaagcagt
     7501 agggattatg acggggcaag tggtctataa atatgaggag gatcaggaaa atgactaata
     7561 cattaacaat tgatcagtta caagagttat tacaaataca aaaggagttc gacgatagaa
     7621 taccaacgct gaacttacga gatagcaaaa tagcatatgt agttgaattc tttgaatggt
     7681 ttaatacatt ggaaacgttt aagaactgga agaagaaacc aggtaagccg ttagatgttc
     7741 agttagatga attagcggac atgttggcgt ttggattgag tattgcgaat caagtaggag
     7801 tgtcatcaga agagataaaa gaagcgattg aatcaagttt taaaaatagt gaatttcaca
     7861 acatgtttaa ttttaaagat aaagaatttg ctcaagacgc agttgttagc acaccacaga
     7921 taatattcaa agaattttat cctgaccaat tggcaattgt aatagcgata gacatagctt
     7981 tcaacttata ttctatcgac cagctcattg acgcatacaa aaagaaaatg aaaaggaatc
     8041 atgaaagaca agatggaaca gcagacgcag gaaaaggata cgtgtaaaga catcttagat
     8101 cgagtcaagg aggttttggg gaagtgacgc aatacttagt cacaacattc aaagattcaa
     8161 caggacgcaa gcatacacac ataactaaag ctaaaagcaa tcaaaggttt acagttgttg
     8221 aggcagagag taaagaagaa gctgagcgca aatacgaggc acaagttaaa agagatgcag
     8281 ttattaaagt gggtcagtta tttgaaaata taagggagtg tgggaaatga cggaggttaa
     8341 aattaaaact atttcagata gagtttatta cacaacaaca gatctagctt ctgacgatta
     8401 tattaatctt gttatgaatc tagtgattga ggattttctt ccggtcaaag atgtgttcaa
     8461 caatgaagta tgggttaaaa gagatgagat tgaatcattt acatttatta aggaggcaaa
     8521 cgatgattaa catacctaaa atgaaattcc cgaaaaagta cactgaaata atcaaaaaat
     8581 ataaaaataa agcacctgaa gaaaaggcta agattgaaga tgattttatt aaagaaatta
     8641 aagataaaga cagtgaattt tacagtccta cgatggctaa tatgaatgaa tatgaattaa
     8701 gggctatgtt aagaatgatg cctagtttaa ttgatactgg agatgacaat gatgattaaa
     8761 aaacttaaaa atatggatgg gttcgacatc tttattgttg gaatactgtc attattcggt
     8821 atattcgcat tgctacttgt tatcacattg cctatctata cagtggctag ttaccaacac
     8881 aaagaattac atcaaggaac tattacagat aaatataaca agagacaaga taaagaagac
     8941 aagttctata ttgtattaga caacaaacaa gtcattgaaa attccgactt attattcaaa
     9001 aagaaatttg atagcgcaga tatacaagct aggttaaaag taggcgataa ggtagaagtt
     9061 aaaacaatcg gttatagaat acacttttta aatttatatc cggtcttata cgaagtaaag
     9121 aaggtagata aacaatgatt aaacaaatac taagactatt attcttacta gcaatgtatg
     9181 agttaggtaa gtatgtaact gagcaagtgt atattatgat gacggctaat gatgatgtag
     9241 aggcgccgag tgattacgaa aaaatcagag ctgaagtttc atggtaatag ctattatcat
     9301 ttttgaatta attatattaa tgtgtttagc aatagcactg gaggtgttgt aaatatgtgg
     9361 attgtcattt caatcgtttt agctatattt ttattgatct tgttaagtag catttctcat
     9421 aagatgaaaa ccatagaagc attggagtat atgaattctt atcttttcaa gcagttagta
     9481 aaaaataatg gtgttgaagg tttagaagat tatgaaaatg aagttgaacg aattagaaaa
     9541 agattcaaaa gctaaagagg gggctaaagc cctctccagg attaaaagaa gcggatattt
     9601 tttatttcgt ttttgtaaat taaaactaat ttatgtgaaa ttgtctttga agtcttgata
     9661 attaagaagg ttga
//
LOCUS       DANJFZ010000097         9456 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.097, whole genome shotgun sequence.
ACCESSION   DANJFZ010000097 DANJFZ010000000
VERSION     DANJFZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.097"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            237..1463
                     /locus_tag="PWN45_002109"
     CDS             237..1463
                     /locus_tag="PWN45_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623856.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            1460..2095
                     /locus_tag="PWN45_002110"
     CDS             1460..2095
                     /locus_tag="PWN45_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623857.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            2112..3053
                     /locus_tag="PWN45_002111"
     CDS             2112..3053
                     /locus_tag="PWN45_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0623858.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            3053..3748
                     /locus_tag="PWN45_002112"
     CDS             3053..3748
                     /locus_tag="PWN45_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623859.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            4042..5442
                     /locus_tag="PWN45_002113"
     CDS             4042..5442
                     /locus_tag="PWN45_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623860.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            5668..6603
                     /locus_tag="PWN45_002114"
     CDS             5668..6603
                     /locus_tag="PWN45_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0623861.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            6925..7776
                     /locus_tag="PWN45_002115"
     CDS             6925..7776
                     /locus_tag="PWN45_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0623862.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(7868..9364)
                     /locus_tag="PWN45_002116"
     CDS             complement(7868..9364)
                     /locus_tag="PWN45_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0623863.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
BASE COUNT         3103 a         1307 c         1895 g         3151 t
ORIGIN      
        1 ttaaatgtcg aatatacgaa tgtgcaaaca aagtaatcgg tagaaattca acatacatag
       61 cgccgtttac tgttaagtat tcacattaca gatgaaaaat ataaaattct acataatcaa
      121 gaccatgatg tgtacttgtt taacttatga ctctatttgt ttaacaattg cgataatggt
      181 ctttttattt tatgcgtatc attcgtcata ttttttatga ggaaggagaa atgattatgt
      241 taagtattaa gcatttaacg aaaatttatt ctggtaataa aaaggcagta gatgacatct
      301 ctttagatat tcaatctggg gaatttatcg catttattgg aaccagtgga agtggcaaaa
      361 cgactgcttt aagaatgata aaccgtatga ttgaagcgac agaaggacaa attgaaattg
      421 atggtaaaga tgttcggagt atgaatcctg tcgaattgcg tagaaatatt ggctatgtta
      481 ttcaacaaat tggcttaatg cctcatatga cgattaaaga gaatattgtg ttggtaccca
      541 aattgttgaa atggactaaa gaggaaaagg ataaacgtgc aaaggaatta attaaacttg
      601 tggatttacc ggagtcattt ttagagcgtt atccagcaga aatatcaggt gggcaacaac
      661 aacgtatcgg tgttgtaaga gcacttgcgg ccgaacaaga tattatttta atggatgaac
      721 cttttggtgc attggatcct attacgagag atacgttaca agatttagtt aaaacgttac
      781 aacgaaaatt aggcaagacg tttatctttg taacacatga tatggatgaa gcgattaaat
      841 tagcagacaa aatttgtatt atgtcagaag gtaaggtggt gcaatttgat acgccagaca
      901 atattttaag acatcccgca aatgattttg tacgtgattt tataggacaa aatagactga
      961 ttcaagaccg tcccaatgac aagactgtag aaggtgtaat gattaaacca atcacgatac
     1021 aagcagaagc aacactgaat gacgccgttc atattatgag acaaaaacgt gttgatgcta
     1081 tttttgtagt agatagtaat aaccatttac taggtttctt agacattgaa gatataaatc
     1141 agggtatacg tggacacaaa agtttacgag acaccatgca acaacatatt tataccgttc
     1201 aaattgattc taaattacaa gattctgtac gtacgatttt aaaaagaaac gttaggaatg
     1261 tacctgtcgt agatgatcaa cagcgtttag taggactgat tacgcgtgcc aatgttgttg
     1321 atattgtata tgacacgatt tggggcgata gtgaggatac agtgcaaaca gaacatgtgg
     1381 gggaagacac tgcgtcctca aaagtgcatg agcaacacac tactaatgtc aaagtacgtg
     1441 acataggaga tgataaatca tgattgagtt cctacatgaa catggtggac agttgatgtc
     1501 gaaaacactg gaacatttct atatttctat agtggcatta ttacttgcca tcattgttgc
     1561 agtacctata ggcattttat tatcaaaaac aaagcgaact gccaatattg tattaactgt
     1621 ggcaggtgtc ttacaaacta ttccaacact agctgtactt gctattatga taccgatttt
     1681 tggtgttggt aaaacgcctg caattgtagc gctatttatt tatgtattat tacctatttt
     1741 aaataacacg gtactcggtg ttcaaaatat tgatagcaac attaaagaag ctggaaaaag
     1801 tatgggaatg acacaatttc aattgatgaa ggatgttgaa ttgccgttag cattgccgct
     1861 tatcattggt ggcattcgtt tgtcatctgt gtatgtaatt agttgggcta cacttgcaag
     1921 ttatgtaggt gcgggtggat taggtgattt cattttcaat ggtttaaatt tatatgatcc
     1981 actgatgatt gtaactgcaa cggtactcgt tactgcacta gcattaggtg ttgatgcctt
     2041 attagcttta gttgaaaaat gggtagttcc caaaggctta aaagtatctg gataattagg
     2101 aggctaagat aatgaagaaa attaaatata tacttgtcgt gtttgtctta tcgcttaccg
     2161 tattatctgg atgtagtttg cccggactag gtagtaagag cacgaaaaat gatgtcaaaa
     2221 ttacagcatt atcaacaagc gaatcgcaaa ttatttcaca tatgttacgg ttgttaatag
     2281 agcatgatac acacggtaag ataaagccaa cattagtaaa taatttaggg tcaagtacga
     2341 ttcaacataa tgccttaatt aatggggatg ctaatatatc aggtgttaga tataatggca
     2401 cagatttaac gggagctttg aaggaagcac caattaaaaa tcctaagaaa gcaatgatag
     2461 caacacaaca aggatttaaa aagaaatttg atcaaacgtt ttttgattcg tatggttttg
     2521 cgaatacgta tgcattcatg gtaacgaagg aaaccgctaa aaaatatcat ttagagacag
     2581 tttcagattt agcaaagcat agtaaagatt tacgtttagg tatggatagt tcatggatga
     2641 atcgtaaagg cgatggctat gaaggattta aaaaagagta tggttttgac tttggtacag
     2701 tgagaccaat gcaaataggt ctagtctacg acgcattaaa ctcagagaag ttagacgttg
     2761 cattaggtta ttctacagat ggtcgaattg cggcgtatga tttgaaagta cttaaagatg
     2821 ataaacaatt tttcccacct tatgctgcga gtgctgttgc aacaaatgaa ttattacggc
     2881 aacacccaga acttaaaacg acgattaata agttgacagg aaagatttcg acttcagaga
     2941 tgcaacgctt gaattatgaa gcggatggta aaggtaaaga acctgctgtc gtcgcagaag
     3001 aatttttgaa aaaacaccac tattttgata aacagaaagg tggtcataag taatggaagg
     3061 taatttatta cagcaattat tcaattatta tgttacgaac tttggttatc tatgggattt
     3121 atttttcaaa cacttattaa tgtctgtcta tggtgtgctg tttgcagctt taattggtat
     3181 tccattggga atcttgcttg caagatacac aaaactttct ggatttgtaa ttacaattgc
     3241 aaatataatt caaacagttc cagtcattgc aatgttagct attttaatgt tagtcatggg
     3301 cttaggttca gaaacagtag ttttaacagt gtttttatat gcgttacttc caattataaa
     3361 aaacacttat actggtatag ctagtgttga tgcgaatatt aaggatgctg gcaaaggtat
     3421 gggaatgaca cgcaatcaag tgctacgaat gattgaatta ccgttatctg tttcggttat
     3481 tatcggtggc attcgtattg ccttggttgt tgcgataggt gttgttgccg ttggatcatt
     3541 tataggagca cctacgcttg gtgacattgt gattcgtggt acaaatgcga cggatggcac
     3601 aacgtttatt ttagcaggtg cgattccgat tgctatcatt gcaatcgtca ttgatgtact
     3661 attaagattt ttagaaaaac gattagaccc aacaacacga catcgtaaaa atcaatctaa
     3721 tcatcggccg caaagtatta atatgtaata gtagaagatg tttataattt agcgatttcg
     3781 tttcatgatt tataaaaaat gaggctactc aaggagctca aataatcttt gagtagcctt
     3841 tttataggtt gtgtttgtat gcgtttacac taaaatagca attattatca tgaaagtttt
     3901 tggataaaaa gcgttaatta ttgtaaaaat actaaaaaat gagatgtttt atttataatt
     3961 ttctgcaaat ttatgatatt gtttcttaat atatcatatt aaaaatttgt ttttcttaaa
     4021 cataggaggc ttatctaatt catggacaca tcaaaacaat ttagaggtga caaccgattg
     4081 cttttgggta tcgttttagg ggttattacc ttttggctat tcgcgcagtc acttgttaat
     4141 cttgttgtcc cattacaatc aacatatagt agtgacgttg gaacgataaa tatcgctgtt
     4201 agcttatctg ccttatttgc tggtttgttt atcgtaggtg ctggtgatgt tgctgataaa
     4261 tttggtcgcg tcaaaattac ttatgtagga ttgatattaa atgttgtagg ttcattactc
     4321 atcatcatta cacctttgcc agcattttta attataggta gaataattca aggtttgtct
     4381 gcagcatgta ttatgccatc aacacttgct attattaacg aatattatat tggtacaaga
     4441 agacaacgtg ccttaagcta ttggtctatt ggttcttggg gtggtagtgg tatttgtacg
     4501 ttgtttggtg gcttaatggc tacatatata ggttggcgtt caatatttgt tgtttcaatt
     4561 ctattaacat tattagcaat gtacttaatc aaacatgcac ctgagactaa agcagaacca
     4621 atcaaaggta tgaaagcaga agctaaaaag tttgacgtta ttggtttagt cattttagta
     4681 gtgacgatgt taagtttaaa tgtaatcatc acacagacgt ctcattttgg tttagtttca
     4741 ccgttaattc taggtttaat tgttgtgttt atctgttcat tagttggttt tgtatattac
     4801 gaaaataaaa taaagcatcc acttgttgat ttttcaattt ttaaaaatag aggatacagt
     4861 ggtgcaacta tttcaaactt cttattaaat ggtgtagcag gtggtgcact tatcgttatt
     4921 aacacgtatt atcaacaaca attaggattt aattcttcgc aaacgggtta tatttcatta
     4981 acgtatttaa taacagtgtt gtcaatgatt cgtgtaggtg aaaagatttt atctcaacat
     5041 ggtccgaagc gcccactatt actaggaagt ggctttacag tgattgggtt aatcttattg
     5101 tcgttaacat ttttaccaga agtgtggtat atcatatcta gtatagttgg atatttattg
     5161 tttggtactg gtttaggatt atatgctaca ccatcaactg atacagcagt tgctagtgcg
     5221 ccagatgata agtcgggtgt tgcttcaggt gtgtataaaa tggcgtcatc attaggaaat
     5281 gcatttggag tagcagtatc tggtacggtt tatactgtgt tagcagctaa tttaaatttg
     5341 aacttaggtg gtttcacagg tatgatgttt aatgccttgc tagcaattgt tgcattttta
     5401 gtcattttac tattagttcc taaaaatcaa acgaatttgt aaaactgaaa tgaaagcaag
     5461 ttattatgta gggattttaa aggaaatttt gtgaaagtaa gtttatcata cacacttaat
     5521 gttgcgtatt gacgtttaat gttaggtgtg ttcttttata gacgataaaa gctgtgtgca
     5581 tattaagcga atgattttca aattgacgct aatatgcgaa agtagtattt ttaaaatgaa
     5641 caacaacgat gaagaggggt ttataggatg aaaattgcaa ttgctggatc gggtgcatta
     5701 ggtagtggct ttggtgccaa actatttcaa gcaggatatg atgtcacact tattgacgga
     5761 tatacatctc atgttgaagc ggttaagcaa catggattaa atataacgat taatggagag
     5821 gcattcgagt taaacattcc gatgtatcat tttaatgatc aaccggacga aagcatttac
     5881 gatgttgtct ttctatttcc aaagtctatg caattaaaag aagtgatgga agatatgaag
     5941 ccacatattg ataatgaaac gatcgtcgta tgtacgatga atggtctgaa gcatgaagaa
     6001 gtcattgcgc agtatgttgc tcaatcacaa attgtcagag gtgttacgac ttggacggca
     6061 ggtcttgaaa gccctggaca cagtcattta cttggtagtg gaccagttga aataggtgaa
     6121 ctagtggatg aaggtaaaga aaatgttata aaagttgctg atttacttaa cgaagcggaa
     6181 ttgaatggtg tcattagtaa agatttatac caatcgattt ggaaaaagat ttgtgttaat
     6241 ggtacggcaa atgcattaag cacagtgttg gagtgtaata tggcatcgct gaatgaaagt
     6301 agttatgcga agtgtttgat ttataaatta acgcaagaaa tagtgcatgt agcgacgatt
     6361 gataatgttc atttaaatgt tgatgaagta tttgaatatt tagttgattt aaatgaaaaa
     6421 gttggtgcgc attatccatc catgtatcaa gatttaattg ttaataatag aaaaactgaa
     6481 attgattata ttaatggcgc agttgcaaca ttaggtaaac aacgtcatat tgaagcgcca
     6541 gtcaatcgct ttattactga tttaattcat actaaagaaa gtcagcgaca cgcacaggat
     6601 taattggtgt tttaggtatg attacaagga aatgacaatc acgtgatatt acggtcatta
     6661 ttaagattga aatgtaataa ataaagaaca gcagtaaggt actttcaaat tgaaatgatc
     6721 ttggtgctgt ttttcttgat tgatcttcgt cataattcag atttgtcata ggtctacgac
     6781 atactattag tatttactag acagttttta cgacgacact ttgaaaaatt ttgaggcaaa
     6841 tcatttggaa gtctcacgtg aattttgtaa actcatcaag caagtaatta tattaaaaag
     6901 acaaatagag aaaaggtgtt tataatgagt aaaatttttg taactggtgc aacgggcctt
     6961 attggcatta aattagttca aagactaaaa gaagaggggc atgaggttgc tggttttact
     7021 acatctgaga atggtcaaca aaagctagct gctgttaatg taaaagcata tattggtgat
     7081 atattaaaag ctgatactat tgatcaagcg ttagcagatt ttaaaccaga aatcattatc
     7141 aatcaaatta cggatttaaa aaatgttgat atggcagcaa atacgaaagt acgtattgaa
     7201 ggttctaaaa acctaattga tgcggcgaaa aagcatgacg ttaagaaagt aattgcccaa
     7261 agtattgcct ttatgtatga acctggcgaa ggattagcaa atgaggaaac ttcacttgat
     7321 tttaactcaa ctggcgatag aaaagtaacg gttgatggtg tggttggttt agaagaagaa
     7381 acggctcgta tggatgaata cgttgtttta cgttttggct ggttatatgg cccaggtact
     7441 tggtacggaa aagatggcat gatttataat caatttatgg atggtcaagt gacactttca
     7501 gatggcgtaa catcatttgt gcatcttgat gatgcagttg aaacatctat tcaagctatt
     7561 cattttgaaa atggtatcta taatgtagca gatgatgcac ctgttaaagg ttctgaattt
     7621 gcagaatggt ataaagaaca acttggtgtt gaaccaaata ttgatattca acctgcgcaa
     7681 ccatttgaac gtggcgtaag caatgagaag tttaaagcgc aaggtggtac tctgatttat
     7741 caaacttgga aagatggcat gaatccaatt aaataataat ttatccgttt aatatacaaa
     7801 gaataaagac ttggtcgaat cgtggatgat atattatcaa acgcacggct cgaacaagtc
     7861 ttttttatta tgtcttcgtt atctttgtat gaaggaataa cagaattaca attaatgtac
     7921 tgaataatgc aattaatgtt gtgattagtg ctaatttaat ttctattggt agccaagtca
     7981 gtacaaaaga ccaattattg ctaccgagaa tgagatatgg taatgcatat aatatgagcg
     8041 ctaaagcgat acatatacat aatgataacc aactcaatac agcaatccgt tttgatctac
     8101 gcaaaagaat ttgaccatgg cgcatttgcc aagcacgata agctgagaat ataaatgcta
     8161 ataaaataag tgttgtcatc aaaacggtaa caatattaaa ttgatcttta tattgattga
     8221 gcatagacgc aaccgtcgaa tatcgcttgt gatttacaat ttgtgtatta agatgctcaa
     8281 ctaatttggg tacatattcc gaatttagat ttgcaagtac aacaattcca taattttgtt
     8341 ttggatttag taaaataaat gatgaaaagt tatctagcgt tcctgaatga aacactaaat
     8401 gttcatcatt attggtaaac cagccggaag catatgcatt ggcattaggt tcaccaattg
     8461 ttgaagataa atttttatgt gattgttgaa ctaatgattt gtatttatca ggtggattaa
     8521 gttggaattt tatccaatgt tccaaatctt cagttgatgt catcatatat gctgatggtg
     8581 tatcccaaag gttaaattca ggtttagaga cgacaggtgt cgaaccttgt aattcatagc
     8641 caatagcatc atgttttgat ttgtaattgg tttgtttgaa tgatgtatgt gtcatatgca
     8701 aaggcttgag ccatgaattt gtaatatatt ttgtatagga ttgcttcgta acgttttgga
     8761 taattaaacc taataaatca tagttcatat ttgagtattc aaattcttct ccgggcttat
     8821 gatgtaattc atcacccata attgcacggg ttacatcatt taaacgatta tttttgcttg
     8881 tcacagaatc ttcgcttgta atatcactag gtataccact tgtttgagcc aaaagttgct
     8941 taatcgtaat agtttcattt tgaccattat agttcatttt aaaatgaggc acatgtttgg
     9001 atacggcatc atttaagttt aatcgacctt cttgagctaa ttttaaaatt gcaagacctg
     9061 tgaaagcttt cgtattagaa gcaatttcat actttgttgt gggagaagct ttgacttttt
     9121 tatcaacatc ttgataacca taacctttat ttaaaaaaac tttgccattt tttactatta
     9181 aaattgatgc tccaggaatg tgtccctttt gtaaatcatg ctcgataatt gtatctattt
     9241 gttgttgcga atcattggtt aaccgtgtct tcgtattgct atttaatgtt atatatatag
     9301 caattgaaat ggcgactaaa atgataatag aaatggatag aaaatacagt tttttagtag
     9361 tcataaacgt tcctttttat atgttaaatt tacttaagtg cctaatgaga tttattttat
     9421 tataccagtt ctattgaata aattaattaa agaggt
//
LOCUS       DANJFZ010000098         9450 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.098, whole genome shotgun sequence.
ACCESSION   DANJFZ010000098 DANJFZ010000000
VERSION     DANJFZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.098"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(239..985)
                     /locus_tag="PWN45_002117"
     CDS             complement(239..985)
                     /locus_tag="PWN45_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDL0623864.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="PWN45_002118"
     CDS             complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="PWN45_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDL0623865.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1529..1786
                     /gene="yidD"
                     /locus_tag="PWN45_002119"
     CDS             1529..1786
                     /gene="yidD"
                     /locus_tag="PWN45_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL0623866.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1783..2784)
                     /gene="menC"
                     /locus_tag="PWN45_002120"
     CDS             complement(1783..2784)
                     /gene="menC"
                     /locus_tag="PWN45_002120"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL0623867.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2789..4267)
                     /gene="menE"
                     /locus_tag="PWN45_002121"
     CDS             complement(2789..4267)
                     /gene="menE"
                     /locus_tag="PWN45_002121"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL0623868.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4426..4881)
                     /locus_tag="PWN45_002122"
     CDS             complement(4426..4881)
                     /locus_tag="PWN45_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDL0623869.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            5184..5834
                     /locus_tag="PWN45_002123"
     CDS             5184..5834
                     /locus_tag="PWN45_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL0623870.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            5915..6910
                     /locus_tag="PWN45_002124"
     CDS             5915..6910
                     /locus_tag="PWN45_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0623871.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            6986..7612
                     /locus_tag="PWN45_002125"
                     /pseudo
     CDS             6986..7612
                     /locus_tag="PWN45_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7653..7997
                     /locus_tag="PWN45_002126"
     CDS             7653..7997
                     /locus_tag="PWN45_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDL0623872.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            8095..8646
                     /locus_tag="PWN45_002127"
     CDS             8095..8646
                     /locus_tag="PWN45_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623873.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(8864..9433)
                     /locus_tag="PWN45_002128"
     CDS             complement(8864..9433)
                     /locus_tag="PWN45_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0623874.1"
                     /translation="MKVIEKVHYEDILESKIVRLFQAFIKDKMDQLFITSEHFAIDID
                     KLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNHSINNTRFSIARELGRYLFKNTDLM
                     RDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQIEALVYNFYEVNNINLSSGLSEKER
                     NKLLNLISTKLEVSKVAAGLRLYNLGIHI"
BASE COUNT         3259 a         1711 c         1460 g         3020 t
ORIGIN      
        1 taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa
       61 aaatttatag gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc
      121 gcatcactca tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac
      181 gcatgtagta ccacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact
      241 agctatgcga ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
      301 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
      361 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
      421 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
      481 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
      541 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
      601 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
      661 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
      721 catttaaatc gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt
      781 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
      841 gtcctacttg acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg
      901 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
      961 gatgcgtaaa atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     1021 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     1081 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     1141 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     1201 tcaatggtat attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat
     1261 aattcacgtg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     1321 tcaatccccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     1381 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     1441 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     1501 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     1561 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     1621 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     1681 ccgtcgtatt ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa
     1741 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     1801 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     1861 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     1921 tttacgagca agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat
     1981 aaccactttt gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     2041 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     2101 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc
     2161 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     2221 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     2281 caattgaaag tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt
     2341 ccattttaat ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga
     2401 taagccgctc gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     2461 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     2521 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     2581 aattacatgt ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt
     2641 acattccccg aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg
     2701 atgcgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     2761 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     2821 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     2881 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     2941 caaaatataa tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt
     3001 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     3061 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     3121 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     3181 gatacaaata tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt
     3241 tattaggatt tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac
     3301 gtgcatgcaa catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa
     3361 atgaattata aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt
     3421 tagcaccgcc gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa
     3481 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg
     3541 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag
     3601 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     3661 tgtcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     3721 acgtttgtgg caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa
     3781 tgtcatctaa attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag
     3841 tttcattcga tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa
     3901 tatcccttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca
     3961 gtggcaaggt acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat
     4021 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta
     4081 ttaaaatgat cgattgaatc gaattatcta tgtatagccc aacacgagat tgttgataag
     4141 ccttgagtct tttagccaat agactcgctt cacagtataa attttgataa gtataagatt
     4201 cttgaccgtc tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa
     4261 agtccatgcg ttattcctcc aaaatcattt acattataat tataacgatt ttatgacatt
     4321 ctagcagtgg ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt
     4381 gttataatgc tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt
     4441 taattttagt gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc
     4501 tgcaatgtca attggtatac ggttcatgtc ttgtaatgca cttaaatgga atacttcatc
     4561 atctaaattt tcaatgagat atacataata tgttaccttg tcctttttat attttaacgt
     4621 tttccaaaag tccggcttgc aattcaatac attatccgga atatattcaa taaataagta
     4681 acgtttgctg cctactttgt ctatgaaata ttttgcagtg cctttttcta tacctcttat
     4741 atgtgcatag tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc
     4801 aaatcgttgg cctactattt tattatttgt gctacaacta cttaaaaaaa tcagtagata
     4861 tagcattaaa catattttca tccccttgaa ttttaaaaac tttttcaaaa gcaacacctc
     4921 taaaaataaa tacaatatat tatagcaaat agaaattatt attttgttaa attatgttgt
     4981 catgttcgtg tcattttgag tcgtaataca actttacaat tattaaatta acacttctac
     5041 aatccaaaaa tatttttaaa gttttgaaaa aaattaaagt atttccacta ctaatttggt
     5101 aaaatatgat tatgtgatat agtgaaagcg ttttacactt aataactccc tcttaaatgc
     5161 atccaggttt tatgtagtaa atcatgaaaa taacatataa atatagagga gatttacctt
     5221 tgaatacaga gaacaacaag aatcaaaacc aatctgttaa aaattctgaa agacgcggca
     5281 tgttaaaagg atgcggcggt tgccttattt cttttatttt attaataatc ttattatcag
     5341 cctgttcaat gatgtttagt aataatgaca attccactaa taatcaatca tcaaaaacgc
     5401 aattaactca aaaagatgaa aataaaaatg aagataagcc tgaggaaaaa tcagaaacag
     5461 caacagatga ggatttacaa tcaaccgaag aagtacctgc aaatgaaaat actgaaaata
     5521 atcaacatga aattgatgaa ataacaacaa aagatcaatc agacgatgat attaacacac
     5581 caaacgttgc agaagataaa tcacaagacg acttgaaaga tgatttaaaa gaaaagcaac
     5641 aatcaagtaa ccatcatcaa tccacgcaac ctaagacctc accatcaact gaaacaaaca
     5701 cgcaacaatc atttgctaat tgtaagcaac ttagacaagt atatccgaat ggtgtcactg
     5761 ccgatcatcc agcatatcga ccacatttag atagagataa agataaacgt gcatgtgaac
     5821 ctgataaata ttaaacaaca agcgaattga attcaaattg tatttagctt tatgcactaa
     5881 tcacatagta aataatgagg gagatttttt aggcatgagc aatcaattca aaagcgaaga
     5941 agagcgaaga caatgggaac aattccaagc tttccaaaat caacaaaacc aacagaacca
     6001 gcaatacgga caaaagaaat ctaaaaaagg atggttctgg ggctgtggtg gttgtctagt
     6061 attatttatt ttaattatca tcggtatttc agcttgtaca gctggtatta caggtaacct
     6121 tggcggaaat agttctaaag aaacgaacaa aacccataaa atcggtgaaa ctgttaaaaa
     6181 tggcgacctt gaagtcactg taaattcagt ggaaactatg aaatctgtag gaccatctct
     6241 tgcaccaaca aacgctaaag gtatatttgt cgttgctgat gtgacgatta aaaacaaagg
     6301 taaagaagcg ttaacaattg atagttcaat gtttaagcta aaatccggtg ataaaacatt
     6361 tgaagcagat aatacaggtt caatgtctgc taatcaaagt gacaatggta gtatagaaaa
     6421 ttcatttttc ttacagcgta taaatccaga tagcactgct caaggtaaaa ttgttttcga
     6481 tgtgtcagaa aacatagcca acgcaaaaga taaaaaatta gaagttattt ctagtttatt
     6541 tagcgtcaag aagattacat ttgatttatc cgatgctaaa aaaacatcaa aagctaaaaa
     6601 agacaagcaa gatacagaag tagctgttgc gagttcaaat agcgataatg taagttatga
     6661 agcttcggct actacacctg ctacaacttc tagtgcggat actgattctg aagatagcga
     6721 aaagtctagt aaagatgagg ataagcagaa tgcgtctaaa agtgataaat ctagtgtaga
     6781 aaaaagtgaa tctaatgagg aaactgctcc tgtagagccc atgccccata gcaaacctac
     6841 cactagtgaa gcaccaccta gccaaaatat tcacaatgaa gatagcatgt acgacgcttc
     6901 aacagaataa aatttcaggg tagctcggct acccttcttt tacggaaaaa ttaattatac
     6961 ataatcaaat caaggagata aaaaaatgaa attcaaagct atcgttgcaa tcacattatc
     7021 attgtcacta ttaactgcct gtggtgctaa tcaacataaa gaaaatagta gtaaatcaaa
     7081 tgacactaat aaaaagacgc aacaaactga caacactaca cagtcaaata cagaaaagta
     7141 aatgacacca caagaagccg aagatatagt tcgaaacgat tacaaagcaa gaggcgttaa
     7201 tgaatatcaa acattaaatt ataaaacaaa tcttgaacga agcaatgaac atgaatatta
     7261 tgttgaacat ctagtccgcg atgcagttgg cacaccatta aaacgttgtg ctattgttaa
     7321 tcgacacaat ggcacaatta ttaatatttt tgatgatatg tcagaaaaag acaaagaaga
     7381 atttgaagca tttaaaaaga gaagccctaa atacaatcca ggtatgaaca atcatgatga
     7441 aacagatggt gagtcagaag acattcaaca tcatgacatt gataataaca aagcaattca
     7501 aaatgacata ccagatcaaa aagtcgacga taaaaatgat aaaaatgctg ttaataaaga
     7561 agaaaaacat gataatgggg caaataattc tgaagaaact aaagttaaat aatggcatac
     7621 tttgattaat cgtaattttt aggagaaacg ctatgataac atttgagaat atacaacaac
     7681 ttgagaaata caccttaatg actatgcatg gtctttttaa tcaacttaaa ctcggcatta
     7741 tttcaatcga aaacgcagag catacgcttt ttacacctta tatgttggaa acgctctctt
     7801 ccctaggcgt gaaagacagc attgtcgatt taattcataa agggactgaa ttagaagact
     7861 ttgcggcatt taatttatca attgaagaca cagttacagt ctgtttacaa agaactgaag
     7921 aactattaaa acaatacaaa aatgtggaat tcaatgacaa aatattaatc aattggcgta
     7981 ttatacaaga gaaatagaca tataaaagtc gaatgtaact acgtgagtat tgattttatt
     8041 ctttgtaaat tacaagcatt tcatattata aagtttgaaa agaggtatat tgaaatggag
     8101 aaaaatgaat atatagctaa atataatgaa tatagtcaat tattagacgc tacatactcg
     8161 caagctgtag catacctttt aagtaaatat ggcgctgtaa ccgatgatta ttataaagaa
     8221 aaatcataca cgcgattttt aaatggagaa atcaaaagta tttcaaaagg aaaatacact
     8281 agagctagcg aaggattata ttgccatcat ataagcgagg acaaattcca aaatctatct
     8341 gatctaagat tcatttccaa atttaagtac tcatacgacg ttcaaaagaa agaaaactta
     8401 gtgtactgtg atctaatcga gcatttaatt ttacatgcaa ttattacaaa agaatcccat
     8461 ggccaatttg gtgtagctgg attatgtcaa atgatcaaac caacagtcat tgattggtac
     8521 attggcgaat ataatccaaa accagcatgg atgcaagcca ccaaagcacg tgcctatttg
     8581 cctggaatat tagtagagaa attactcatt aaaattgacg atatgttaaa aggaatagaa
     8641 atataagatt tccttgagtc tagataaatg attaatgtag atttattttt tgctgttgag
     8701 attttgttat agatgtttaa acctgtaatt aaatatattt tataaaatag accacgcata
     8761 cctatctata aacggacaat gtttataaat gagtttgcat ggtcttgaat tgtattaaat
     8821 ttcttttggt tttaataaat cgactagatt ttcacaatat ttatcaaata tgtattccta
     8881 aattatacag ccttaatcca gcagctactt tcgaaacttc caacttagtt gatataaggt
     8941 tcaatagttt gtttcgttct ttttcagata aaccagaact taaattgata ttattgactt
     9001 cataaaaatt atagactaat gcctctattt gctttttagg cataagtaag tcgactgaaa
     9061 actgatttac gtcgctttca taaatcattt catgtaaatt ctttagacta ttatcgttac
     9121 tatctctcat taagtctgta tttttaaata aataacggcc caattcacga gctattgaaa
     9181 atcttgtatt attaatcgag tgattattat taatatagat tgttcttcca cttaaataac
     9241 ccgaagtatt accctccatt ttaatatatc taacatttaa attaagttga aataatagct
     9301 tgtctatgtc aatagcaaag tgttcagaag taataaaaag ttgatccatt ttgtccttta
     9361 taaatgcctg aaataatcga actatttttg attctaaaat atcttcataa tgaactttct
     9421 caataacttt caattgattc cccaccctaa
//
LOCUS       DANJFZ010000099         9379 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.099, whole genome shotgun sequence.
ACCESSION   DANJFZ010000099 DANJFZ010000000
VERSION     DANJFZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.099"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            156..608
                     /gene="argR"
                     /locus_tag="PWN45_002129"
     CDS             156..608
                     /gene="argR"
                     /locus_tag="PWN45_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL0623875.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            624..2303
                     /gene="recN"
                     /locus_tag="PWN45_002130"
     CDS             624..2303
                     /gene="recN"
                     /locus_tag="PWN45_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL0623876.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            2454..3875
                     /gene="lpdA"
                     /locus_tag="PWN45_002131"
     CDS             2454..3875
                     /gene="lpdA"
                     /locus_tag="PWN45_002131"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL0623877.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3891..4883
                     /locus_tag="PWN45_002132"
     CDS             3891..4883
                     /locus_tag="PWN45_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDL0623878.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4883..5866
                     /locus_tag="PWN45_002133"
     CDS             4883..5866
                     /locus_tag="PWN45_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL0623879.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5879..7153
                     /locus_tag="PWN45_002134"
     CDS             5879..7153
                     /locus_tag="PWN45_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDL0623880.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7504..7941
                     /locus_tag="PWN45_002135"
     CDS             7504..7941
                     /locus_tag="PWN45_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDL0623881.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7955..8935
                     /locus_tag="PWN45_002136"
     CDS             7955..8935
                     /locus_tag="PWN45_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0623882.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(9062..9241)
                     /gene="prli42"
                     /locus_tag="PWN45_002137"
     CDS             complement(9062..9241)
                     /gene="prli42"
                     /locus_tag="PWN45_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDL0623883.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         3464 a         1280 c         1780 g         2855 t
ORIGIN      
        1 agcaattgtt atgcgtaata tgaaaaatat ttataaatag ataaccaaga tagataaacc
       61 atgtttaccc tttttacgta aaggtgttta gtagtcgtaa atcagtgcat gtgttaaaat
      121 aaatgtataa atattcgtaa tcagaggtgt ttactgtgcc caaaaaatcg gttaggcata
      181 taaaaattag agaaattatt tcaaatgaac agatagagac acaagatgaa ttagttaaac
      241 gattaaacga ttatgattta aatgtcactc aagcaactgt ttctcgtgat attaaagaac
      301 tacaacttat taaagtacct ataccttcag gtcaatatgt ttatagttta ccaaatgata
      361 gaaaattcca tcctttagaa aaattgggac gttatttaat ggattccttt gttaatatag
      421 atggtactga taatttactt gttctaaaaa cattacctgg taatgcacaa tctattggag
      481 ctatattaga ccaaatcaat tgggaagaag tactaggcac aatttgtggt gatgatactt
      541 gtttaattat ttgtcgaagc aaagaggcaa gtgatgaaat caagtcaaga attttcaatt
      601 tgttataagg atgcgatgag tttatgttac aaaccttatc aatcaagcaa tttgctatta
      661 ttgaagaatt agaaattcaa ttttctgatg gtttaactgt tttaagtggt gaaacgggtt
      721 caggtaaatc aattattata gatgcaattg gacaattaat tggaatgaga gcttcatctg
      781 attttgtaag acatggtgaa aaaaaggctg tcatcgaagg tatatttgat attgacgaga
      841 gtaaagatgc tatacatata ttgaaaaata tggatattga tgtagatgaa gattttttat
      901 tagttaaacg ggaaattttc agctctggta aaagtctttg taaaattaat aatcaaacag
      961 ttactttaca agatttacga aaagttatgc aagaattatt agatattcat ggccaacatg
     1021 aaacgcagtc tttgttgaaa caaaaatacc atttaacatt attggataat tacgcagaat
     1081 cacgttatca agatttgttg gataaatatc accaaacttt tcaaaattat aaagccaaaa
     1141 agcaagagtt agaagattta gaatcagcag accaagcatt gctacagcgt ttagatttaa
     1201 tgaaattcca attagaagaa ttgtctgaag cacatctaaa agaaggcgaa atagagcaac
     1261 ttgaaataga tattaaacgc attcaaaact ctgaaaaatt aagcttagcg cttaataacg
     1321 cacatatgac attaacggat gaaaatgcta tcactgatcg tttatatgaa ctaagcaacc
     1381 atttgttaac aataaacgat atagttccaa acaaatatga caaattaaaa gaagatattg
     1441 atcaatttta ctacatttta gaagatgcaa aacatgagtt atatgatgaa atggctaata
     1501 cagaattcga cgaacaagtg ttaaacgagt atgaatctcg tatgaatttg ctgaataatt
     1561 taaaacgtaa atatggaaaa gatatttcag aattaatcgc atatcaagaa aagcttaata
     1621 atgaaatcaa taaaattgaa aactatgaac aaagtacatc gcagttacga gaagaaataa
     1681 atgcattgta taatcaagtt atagaggttg gacaagcgtt atcaaagcag cgtcgtatag
     1741 tcgctaggga gttaagagac cacattgtat cggaaattca aaacttacaa atgaaagacg
     1801 caaatcttga gatttcattt aaaaaattag aagaaccgaa tattgatgga atcgaatttg
     1861 tagaattttt aatcagtcca aataaagggg aaccattaaa aagtttaaat aaaattgcgt
     1921 caggtggaga actttctaga attatgttag cgttaaaaag tatttttgtt aaatcgagag
     1981 gtcaaactgc aattttattc gatgaggttg actcaggtgt atctggacaa gctgcacaaa
     2041 aaatggcaga gaaaatgcga gatatagcag aatatataca agttatttgt atctctcact
     2101 taccacaagt tgcatcgatg agcgaccatc atttattaat ttcgaaatca tcaaaagatg
     2161 atcgtacaac aacacaagtt caagagttaa ttagcgatga taaagtagat gaaattgcac
     2221 gaatgatttc aggtgcaagt gttactgatt taactcgaga aaatgcaaga gaaatgatac
     2281 aacacaatca aagacgtaga taatttccaa agtgtgttga atactcattt atggatttat
     2341 ttatgtgcat tcaaactaaa tagacaatca tgtaaaatag gtaattaatg gacatgaaaa
     2401 ctatgtatta ataaattttc aatggatata tatcattagt taggagtaag aacatgtcag
     2461 agaaacaata tgatttagtc gttctcggtg gaggtactgc aggatatgtt gctgcgatac
     2521 gtgcgtctca attaggtaaa aaagtggcaa tagtggaacg acaactatta ggaggtactt
     2581 gtctccataa aggttgtatt cctactaaat cgctgcttaa atctgctgaa gtatttcaaa
     2641 cagtaaaaca ggcagcaatg tttggcgttg atgtcaaaga tgctaatgtt aattttgaaa
     2701 atatgttggc acgaaaagaa gacattatta atcaaatgta tcaaggtgta aagcatttaa
     2761 tgcaacacaa tcacattgac atttataatg gcacgggacg tattctaggt acatctatat
     2821 tttcacctca aagtggcaca atttctgtcg aatatgaaga tggtgaatca gacttattac
     2881 ccaaccaatt tgtattaatt gcaactggtt cttcgcctgc agagttgcca ttcttatcat
     2941 ttgatcatga taaaatttta tctagcgatg atatattatc attaaagacg ttaccatcaa
     3001 gtattggtat tattggtggt ggtgttatcg gaatggaatt tgcatcattg atgatagatt
     3061 taggtgttga cgtaacagtt atagaagcag gtgaaagaat tttaccaact gaaagtaaac
     3121 aagcttcaca actattaaaa aaatcattgt cagcacgtgg tgttaaattc tatgagggaa
     3181 taaaactttc tgaaaatgat ataaatgtta acgaagatgg tgttacgttt gaaatttcct
     3241 cagacataat taaagtagat aaagtgttgc tatcgattgg tagaaaacct aacacatcag
     3301 atattggttt aaacaacact aaaataaaac tttcaacatc aggacatatt ttaacgaacg
     3361 aatttcaaca aactgaagat aaacatattt atgcagctgg tgattgtata ggaaaattac
     3421 aattggcaca tgttggatca aaagaaggtg ttgtggctgt tgatcatatg tttgagggga
     3481 acccaatccc agtaaactat aacatgatgc ctaagtgtat ttattcacaa cctgaaattg
     3541 cttctatcgg tttaaacatt gaacaagcaa aggcagaggg aatgaaagtt aaaagtttta
     3601 aagtaccatt taaagcaatt ggtaaagcag tgattgatag ccatgacaca aacgaagggt
     3661 atagcgaaat ggtgattgat caatcaactg aagaaattgt gggtattaat atgattggtc
     3721 cacatgtaac agaattgatt aatgaggcat cactgttaca gttcatgaat ggctcggcat
     3781 tagaattagg actaacaaca cacgcacatc cttccatctc tgaagtgttg atggaattag
     3841 gattgaaagc agaaagtaga gctattcacg tataaatagg aggatttaac atgattgatt
     3901 ataaatcatt aggccttagc gaagaagacc taaaagtaat atataaatgg atggatttag
     3961 gtagaaaaat agatgaaaga ctgtggttat tgaaccgtgc aggtaaaatt ccatttgttg
     4021 taagtggtca aggacaagaa gcaactcaga ttgggatggc atacgcatta gaagaaggtg
     4081 atattacagc gccttattat agagatctag ctttcgttac ttatatgggt atctcagcgt
     4141 atgatacgtt tttatcagcg tttggaaaga aagacgatgt taactctggt ggaaaacaaa
     4201 tgccatcaca ctttagtagt agagctaaga atattttgtc tcaaagttca ccggtagcaa
     4261 ctcaaatacc ccatgctgtt ggtgctgctt tagctttaaa aatggatggt aagaagaaaa
     4321 ttgcaacagc aactgttggt gaaggtagct caaaccaagg tgacttccat gaaggtttaa
     4381 acttcgcggg tgtacacaaa cttccttttg tatgtgtcat tataaataat aaatatgcaa
     4441 tttcagtacc tgattcatta caatacgcag ctgaaaaatt atcggataga gcacttggtt
     4501 acggtataca tggtgaacaa gtagatggta atgatccatt agcaatgtat aaagcgatga
     4561 aagaagcaag agatcgagct atttctggtc aaggttcaac tttgattgaa gcagtaacaa
     4621 gtcgtatgac agctcattca tcagatgatg acgaccaata tcgtacgaaa gaagagcgtg
     4681 aaacgcttaa aaaagcagac tgcaatgaaa agttcaaaaa agagttgctt tcagctggca
     4741 ttatcgatga tgcttggttg gcagaaatag aagcggagca taaagacatc atcaataaag
     4801 cgacaaaggc tgctgaagac gcgccatatc caagtgtcga agaagcctat gcatttgtgt
     4861 acgaagaagg gagtcttaac taatggctaa attatcttat ttagaggcga tacgccaggc
     4921 acaagactta gcattacaac aaaataaaga tgtgtttatt cttggtgaag atgttggtag
     4981 aaaaggtggc gtatttggta ctactcaagg actgcaacaa aaatatggtg aagacagagt
     5041 tatcgatacg ccattagctg agtcaaatat tgttggaact gccattggtg cagctatggt
     5101 aggtaaaaga ccaattgcag aaattcaatt tgcagatttt attttacctg cgacaaatca
     5161 aattattagt gaagctgcga aaatgcgtta tcgttcaaat aatgattggc aatgcccatt
     5221 aacgattcgt gcaccatttg gtggcggtgt tcacggagga ctatatcatt cacaaagtat
     5281 tgaaagtata tttgcttcat cgccaggttt aacaattgtt attccttcca caccgtatga
     5341 tgctaaaggg cttttattat catctatcga atcaaatgat ccagtattgt actttgagca
     5401 taaaaaagct tatcgtttct taaaggaaga agtgccagaa gaatattaca ctgtaccttt
     5461 aggaaaagca gatgtgaaac gtgaaggtga agatttaaca gtattttgtt atggattaat
     5521 ggtcaattat tgtttacaag cagcggatat tttagctgca gacggtatca atgttgaagt
     5581 cgttgattta cgaactgttt atccattaga taaagaaaca attattgatc gtgctaaaca
     5641 tacaggtaaa gttttacttg taactgaaga taatttagaa ggtagtatta tgtcagaagt
     5701 gtcagcgatt attgcagagc attgcttgtt cgatttagat gcaccaatca tgcgtttagc
     5761 tgctccagat gtaccatcta tgccattttc tcctgtatta gaaaatgaaa ttatgatgaa
     5821 tccagaaaaa atcttaaata aaatgcgtga attagcagaa ttctagggag ggaaagtcat
     5881 ggaaataaca atgcctaagt taggtgagag tgttcatgaa ggcaccattg aacaatggtt
     5941 agtttctgtt ggtgatcata ttgatgaata tgaaccatta tgtgaagtta ttacagataa
     6001 agtgacagct gaagtccctt ccacgatatc aggaacaatt acagaaattt tagttgaagc
     6061 ggggcagaca gtagctattg atacaattat ctgtaaaatt gaaactgctg atgaaaagac
     6121 aaatgaaaca actgaagaga tacaagcaaa agtggatgag catactcaga aatctactaa
     6181 aaaagctagt gcaacagtgg aacagacatc tactgctaaa caaaatcaac cacgtaataa
     6241 tggtcgcttt tcacctgttg tatttaaact cgcttcagag catgacattg atttatcaca
     6301 agttgtaggt agtggatttg aaggtcgtgt aactaagaag gatataatgt cagttattga
     6361 aaatggtggt accacagctc aatctgacaa acaagttcaa acaaaatcaa catcagtaga
     6421 tacatcaagt aaccaatcat ctgaagacaa tagtgaaaac agcacaatac cagtaaatgg
     6481 tgtgcgtaaa gcaattgcgc aaaatatggt taatagtgta acagagattc cacatgcatg
     6541 gatgatgatt gaagtagatg ctacaaatct tgtgaatacg agaaatcatt ataaaaacag
     6601 ctttaaaaat aaagaaggat ataatctaac gttctttgct ttctttgtaa aagctgtagc
     6661 agatgcttta aaagcatatc ctttattaaa tagtagctgg caaggaaatg aaattgtctt
     6721 acataaagac attaatattt caattgctgt tgctgatgaa aataaattat acgtacctgt
     6781 gattaagcat gcagacgaaa agtcaatcaa aggtatagct agagaaatta atactttagc
     6841 aacgaaagcg cgtaataagc aattgacagc tgaagatatg cagggcggta catttacggt
     6901 aaataatact ggtacatttg gttcagtatc atcaatgggt attataaatc atccacaagc
     6961 agcgatttta caagtagaat caatcattaa aaagccagta gtaattaatg atatgattgc
     7021 aattcgtaac atggttaatt tatgtatttc aattgatcat cgtattttag atggtttaca
     7081 aacaggtaaa tttatgaatc atattaaaca gcgtatcgaa cagtatactt tagaaaatac
     7141 aaatatatat tagtgataac atagatgcat ctatcgacaa cttgttttat cttgttcttg
     7201 tcgatggatg tattattttt tggcactaaa atatgtgcaa tatattcaaa aaagataaag
     7261 aacaataatc aacatggttg aatgcatttt tgcagtaaag tcaaaataag acatcatact
     7321 tgaaacatat taatgaaaac atgtgaacaa attagttacc atgattttaa gcacaataat
     7381 gtttggtata ttgttaaaat tgtgtctaaa tataggtgtg attcagatta gtttattgaa
     7441 caatatgtta ttaattagta gaatgaggat agtttaaata taaagggata ggtgattgaa
     7501 cttatggaca tgaatttcga tttatacatg aacggtgttg tagaacaagc aaggaatgaa
     7561 attgaatctg cgggatatga gcaattaact actgcagaag atgttgacaa agttcttaaa
     7621 caagatggta caacactagt tatgatcaat tctgtatgtg gttgtgcagg tggtatcgca
     7681 agaccagcag catcacatgc tttacattat gacgtattac ctgatcgtct agtgacagta
     7741 tttgctggac aagataaaga agcgacacaa agagcgcgtg aatacttcga aggttatgcg
     7801 ccttcaagtc cgtcatttgc attagtaaaa gatggaaaga ttacagaaat gattgaaaga
     7861 catcaaatcg aaggtcatga tgtgatgaac gtaattaatc aattacaaac attattcaat
     7921 aaatattgtg aagaaagata agaggcgcta acccatgtta aagttaaatc cttacaagat
     7981 tggatttaga acaataaaaa cagcagtggg tatgacttta ggtgtaatta ttagtaagct
     8041 gttaggttta gataattatg cttcaagcgc catattagtc gtattatgta ttaaacatac
     8101 aaaagtacat tcgctacaag cgattatttc aagattagta tcatgttttt tagtattgtt
     8161 tttaggttca gcaatattta gtttattagg tcagagtcca attgtactcg gtattatcgt
     8221 attgttattt ataccattaa ctgtcgtatt aaaagtacaa gaaggtgtca ttacgagttg
     8281 cgttatatta cttcatgttt ttaatgcaaa atcaattgat gcacatttaa ttgttaatga
     8341 aacattatta ctgttaattg gactaagcat tgcatttaca atgaatttaa tgatgccaag
     8401 tttagacaaa caactagacg aatacaaatg taaaattgag caacaaattg ctgatatttt
     8461 tagtaaatat agttatattt gtgaaaaata tgaagatacc attgcgattg aatttgaagt
     8521 gttactttta aatattaaaa aggcgaagtc tatcgcgttc cgagatgtta aaaatcattt
     8581 tgttagaaac gaaaattcat actatcatta ttttgatatg cgagaagagc aagtggaatt
     8641 gttaatgaga atgaaaccgc tcatcgaaag tatctgtcat aaagatccgc ttttaaatga
     8701 tgttgcgtca ctgctgaaag aaattggtct aaatgtgaat agtaatgact atacagcaat
     8761 gcgtttacac aatttgtatg agacgcgttt gaaattggat gaactgccat tgccaacaac
     8821 acacaaagcg cttaactcga gagcaagtat tattcaaata ttaaatgaat tagaagaata
     8881 tttacaaatc aaatcgcaat ttggttcatt gaaaatgcat agtgaagtag cttaaaaaat
     8941 aatccatccg tatgagccat gtttgaaagt aatcgttgca atacgataac ttattaatac
     9001 atgcgtttat aatggatgga tttttaattg tatgattttg tataggtagt tgagatatag
     9061 tttataaaat tggaatgata ctcgttaata acaatgctaa tacaactgat acaagcataa
     9121 cgataatgat aattcttaaa actttatttt gcattttgtg tgcctccaaa tgaataacct
     9181 ttgtattaat aattacgaca taagatataa aacttttaac ttgagacgat ataactagca
     9241 atcatcataa aggaatataa taaaaaacat gagtaagttt gcttatgtca ttttcctcat
     9301 tatacgatat ctttgaaaaa tcaatcaata tatttttgac aaagcttagt ttgagttaca
     9361 ataattatat ataataaat
//
LOCUS       DANJFZ010000100         9039 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.100, whole genome shotgun sequence.
ACCESSION   DANJFZ010000100 DANJFZ010000000
VERSION     DANJFZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.100"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            23..1099
                     /locus_tag="PWN45_002138"
     CDS             23..1099
                     /locus_tag="PWN45_002138"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL0623884.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1349..2032
                     /locus_tag="PWN45_002139"
     CDS             1349..2032
                     /locus_tag="PWN45_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0623885.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2148..2810)
                     /locus_tag="PWN45_002140"
     CDS             complement(2148..2810)
                     /locus_tag="PWN45_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0623886.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(2803..3420)
                     /locus_tag="PWN45_002141"
     CDS             complement(2803..3420)
                     /locus_tag="PWN45_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0623887.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            3487..3957
                     /locus_tag="PWN45_002142"
     CDS             3487..3957
                     /locus_tag="PWN45_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDL0623888.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            4104..4973
                     /locus_tag="PWN45_002143"
     CDS             4104..4973
                     /locus_tag="PWN45_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0623889.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            4994..5560
                     /locus_tag="PWN45_002144"
     CDS             4994..5560
                     /locus_tag="PWN45_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0623890.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            5991..8834
                     /gene="sdrC"
                     /locus_tag="PWN45_002145"
     CDS             5991..8834
                     /gene="sdrC"
                     /locus_tag="PWN45_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HDL0623891.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         3421 a         1308 c         1605 g         2705 t
ORIGIN      
        1 atattattag gaggcaatca ccatgtcaca agcagttaaa gttgaacgac gagaaacatt
       61 aaaacaaaaa ccaaatacat ctcaactagg ttttggtaaa tattttactg attatatgtt
      121 gagttatgat tatgatgcag ataaaggatg gcatgatttg aagatagtac cttatggtcc
      181 tattgaaatt tcacctgctg cacaaggtgt tcattatggt caatcggtat tcgaaggatt
      241 aaaagcatat aaaagagatg gggaagttgc acttttccgt cctgaagaaa attttaagcg
      301 tcttaataac tcgttagcac gattagaaat gcctcaagta gacgaagcag aattgttaga
      361 ggggctaaaa caattagttg atattgaaag agattggatt cctgaagggg aaggtcaatc
      421 attatatatt cgtccatttg tttttgcaac agaaggggca cttggcgttg gtgcatcaca
      481 tcagtataaa ttattaatta ttttatctcc ttcaggtgca tattatggtg gtgaaacttt
      541 aaaaccaact aaaatctatg tagaagatga atatgtgcgt gctgttcgtg gcggtgtagg
      601 ctttgcaaaa gttgcaggta actatgcggc aagtttatta gcacaaacta atgcaaataa
      661 attaggttat gaccaagtat tatggcttga tggtgttgaa cagaaatata tcgaagaagt
      721 tggtagcatg aacattttct tcgttgaaaa tggcaaagta attacaccag agttgaatgg
      781 cagtatttta cctggtatta cacgtaaatc tattatcgaa ttagctaaaa acttaggata
      841 tgaagtcgaa gagcgccgcg tttcaatcga tgaattattc gaatcatatg ataaaggtga
      901 gttaacagaa gtatttggta gtggtactgc agcagttatt tcacctgtgg gtacattgag
      961 atacgaagat cgtgaaatcg ttattaataa taatgagact ggtgaaatta ctcaaaaatt
     1021 atacgacgtc tatactggta ttcaaaatgg tactttagaa gataaaaatg gttggagagt
     1081 cgttgtacca aaatattaat aaaaattgaa tataatcatg aaaacaatat gcaaaagtta
     1141 atcatgacta aaatcccttt caatgaagcg aagcatggta ataaattaag tttacaatgt
     1201 ttattgttaa gtattgaagg ggatttcact tattattata tttaattcaa tatttaaata
     1261 gaacaatact acgagttcat tttcagggga taacgaatat catagaatca gcataatacg
     1321 aaataaataa ataggagtat tgatatcaat ggaatggata ttatttgata aagatggtac
     1381 gttaattgaa tttgatagaa gttgggaaaa aataggggta cgatttgtac aatcattgct
     1441 tgagactttc ccagtacata ataaagaagc tgctttaaga caactcggtg tcattaaaga
     1501 atctattgat ccaaaatcag tgatgggttc aggatcttta caacaaatta tccaggcatt
     1561 taatgatgtg acgggacaag atacaaccga ctggtccaag tcaacaagtc aaaagctggt
     1621 agatgaacgt attcctgaaa ttaattgggt agaaggtgtt aaagaagcac ttatcgattt
     1681 gaaagcaaaa ggctatcaac ttggtattgt tacgagtgat actaaaaaag gtgtagaaca
     1741 atttttagca cataccaatg ctacctcgtt gttcgatttg atcatttcta ccgaagcgga
     1801 tgcctatgag aagccaaatc ctaaagtatt atcgccttta tttgagcaat ataatgtaga
     1861 tcctcagaaa gtagctatag taggagacac tgctaatgat atgaagacag caagtaatgc
     1921 aaatttaggt atggcaatag gtgtattaac aggtattgca acaaaagaag aattacatga
     1981 agctgatatt attttaaata gtgcggcaga tattttagaa gctttaaatt aaaaagaaag
     2041 acatagtgta tatgtgaatt aatgtattcg atagaattga taaaataatc gataatatct
     2101 atggaatata cattgaatat aaacatatac gctatgtctt tagtctttta tcgtgtatct
     2161 acttgtcgat atgtttgaat aattcgagca attttgttta tcataggatt taaagattcg
     2221 gggtccttat ggatatcata ttcattaata ttgatacgta caactggaca tgcattaaag
     2281 ctattaatcc aatcgtcata gcgtttaaat agctttttcc agtattcagg gtctgtatta
     2341 atttccattt cgcgaccacg ttcaataata cgatcaatga cctcatcata gttacattct
     2401 aaataaatca ttacatcagg tttaggaaaa taaggtgtca tgaccatggc attaaataag
     2461 tctgaatatg ttttgaaatc ttctttactc attgtgcctt cttcttcatg catttttgca
     2521 aaaatatcaa catcttcata aattgatcga tcttggacaa agccaccacc atattcaaac
     2581 atacgctttt gttctttaaa acgttcagct aagaagtaaa tttgcaaatg gaaactccat
     2641 cgttcaaaat cgctgtaaaa tttatctaaa tatggattat gttcgacatt ttcaaaagac
     2701 gttttaaagt ttaatttatc tgcaagtgct tgcgttagtg ttgattttcc aacaccaact
     2761 gtacctgcaa tggttataat ggcattttgt ggaataccgt aattattcat tggtaatatc
     2821 tcctatcata ggtaatataa tatgtaatat atcttcgtaa tcttgttcat ttttaagaaa
     2881 atcaatagaa gttgtatcga ttaaaactac atttgaacca ttactttgta aggactcata
     2941 atactcacga taatcttttt ttaactttaa cagatattca tcttctattt gatgctcaaa
     3001 actacggtta cgtttagcaa ttctagattt taacacatca aggtctgcat ctaaaaagat
     3061 aatcatattc ggcataatca tatcttcagt taaaatatca taaattttac tgaatttctg
     3121 aaattcaaca gaactcaaag tatttttagc aaatatctta tttttatgta tatgataatc
     3181 actaactaca ccttgattta gttgtgttac atcttgaaat tgcttatatc tattgcataa
     3241 aaagaacatt tcagtttgaa aactccattt agagatatct tcataaaagt ctgataaaaa
     3301 tggattttct gtgatgattt ctttttcttc ataaaaatct aaagtttgac ttaatttgtg
     3361 tgcaagtgaa gatttaccta cgccaatagg accttcaatt gctataaaag gtttgttcat
     3421 attcacatct ccaaatgtta aaatagacat tgagtattgt accataagtg aggtatctgg
     3481 acataaatga caaatgatat atattttatg acattagcga ttgaagaagc taaaaaagca
     3541 gctcaactag gcgaagtacc tataggtgct atcatcacta aagatgatga agttatcgct
     3601 agagcacata atttaagaga aacactacaa caaccaacgg cgcatgctga acatattgca
     3661 attgaacgtg cagccaaagt gttaggtagt tggcgtttag aaggttgcac attatatgta
     3721 accttagaac catgtgtcat gtgcgcagga acaattgtaa tgagtcgcat tccaagagtc
     3781 gtctatggcg cagatgatcc taaaggtggt tgtagtggca gtttaatgaa tttattgcaa
     3841 caatctaatt ttaatcatcg tgcaattgtt gataaaggtg tacttaaaga agcatgtagc
     3901 acattattaa caacattttt taaaaactta agagccaata agaaatccac caattagacg
     3961 atagtacata ctaattaaaa tttgttagaa ttacattata tgataaatta atgacaatat
     4021 aaatgttaat agaaaagtat gtgcgctgag tatatattct tatttaagaa caagatttta
     4081 aatttacgaa acgaggtaac ataatgataa aactaatagc cactgatatg gatggcacgc
     4141 tacttaatgc agcacatgaa atttctcaac ctaatattga tgcgattaaa tacgctcaag
     4201 aacaagggat aacggttgtt atcgcgacag gtcgagcatt ttatgaagca caagcaccag
     4261 ttgctgacac agatttaaca gtaccatata tttgtttgaa tggtgctgaa gtacgtgatg
     4321 aaactttcaa tgtaatgagc acttcacacc ttaataaatc gttagtacac aaaattacaa
     4381 atgttttaaa agatgcaggt atttattatc aagtatacac gagtcgtgcg atttatactg
     4441 aagatccaca aagagattta gacatttaca tagatattgc tgagcgtgca ggtcaacatg
     4501 caaacgttga gcgtattaaa aatggtattc aaagacgcat agataatggt acgttgaaag
     4561 ttgttgataa ttatgatgct attgaaaaca tacctggtga attaattatg aaaatattag
     4621 catttgatgg aaatttagaa aaaattgaca aagctagtaa aattttagct gaatctccga
     4681 atttagctat atcatcatct tcgagaggaa atatagaaat aacgcattca gatgcacaaa
     4741 aaggtattgc gctagaaaca attgccgaaa gattagggat tgaaatgaaa gaagtcatgt
     4801 caataggtga caatttaaat gacttatcaa tgttagagaa agttggctat ccagttgcga
     4861 tggaaaatgg tgcagaagaa gttaaaaaaa tagcgaaata tgtcacagat acgaatgaaa
     4921 atagtggtgt tggaaaagct attatgaaat tattacgtga acaacaagtt taataaaaaa
     4981 agaggggtca aatatgaaag gattaattat tattggcagt gcacaagtga attcacatac
     5041 aagtgcacta gcaagatact taactgagca ttttaaaaca catgatattg aagcggaaat
     5101 attcgattta gcagaaaaac cgttaaatca attagatttt tcaggaacaa caccgtctat
     5161 tgatgaaatc aaacaaaata tgaaagattt aaaagagaaa gcaatggcgg cggacttttt
     5221 aatattagga acgccaaact atcatggttc atattctgga atattgaaaa atgcattaga
     5281 tcatctaaat atggattatt ttaaaatgaa acctgtaggc ttaataggaa atagtggtgg
     5341 tattgttagt tcagagccat tgtcacattt aagagtaatc gtcagaagtt tactaggcat
     5401 tgctgtacca actcaaatag caacacatga ttctgatttt gctaaaaatg aagatggttc
     5461 atattactta aatgatagtg aattccaatt acgagcaaga ttatttgtcg atcaaattgt
     5521 atcttttgtg aataatagtc catatgaaca tttaaaataa tattaaaaaa tatgtaaata
     5581 tacattaaaa ataggtatgt agcttaaggt atttcattga gaatgctttt aagtcacatg
     5641 cctatttttt gttctaatgc accaatagta agcctttgat attactgttg tgagcaatgt
     5701 tattaattaa aataagatgt aatagcgaat tgaaaacaag cttagattaa aagtgagttt
     5761 taaaaatata gataaataat ttaaagcaat taaagaaaaa aagtattaaa aattgcaatt
     5821 cttaaactgt gattcatatt aatatttcta gcaaataaca ttgtaaaata aagaaaaata
     5881 attaaagtat tgcactttat tgaaatttat attacgatag taatgcagaa atttatatat
     5941 gcaaaatatt atatttatca aattttgata ttttaaagga gtattattaa atgaataata
     6001 aaaagacagc aacaaataga aaaggcatga taccaaatcg attaaacaaa ttttcgataa
     6061 gaaagtattc tgtaggtact gcttcaattt tagtagggac aacattgatt tttgggttaa
     6121 gtggtcatga agctaaagcg gcagaacata cgaatggaga attaaatcaa tcaaaaaatg
     6181 aaacgacagc cccaagtgag aataaaacaa ctaaaaaagt tgatagtcgt caactaaaag
     6241 acaatacgca aactgcaact gcagatcagc ctaaagtgac aatgagtgat agtgcaacag
     6301 ttaaagaaac tagtagtaac atgcaatcac cacaaaacgc tacagctaat caatctacta
     6361 caaaaactag caatgtaaca acaaatgata aatcatcaac tacatatagt aatgaaactg
     6421 ataaaagtaa tttaacacaa gcaaaagatg tttcaactac acctaaaaca acgactatta
     6481 aaccaagaac tttaaatcgc atggcagtga atactgttgc agctccacaa caaggaacaa
     6541 atgttaatga taaagtacat ttttcaaata ttgacattgc gattgataaa ggacatgtta
     6601 atcagactac tggtaaaact gaattttggg caacttcaag tgatgtttta aaattaaaag
     6661 caaattacac aatcgatgat tctgttaaag agggcgatat atttactttt aaatatggtc
     6721 aatatttccg tccaggatca gtaagattac cttcacaaac tcaaaattta tataatgccc
     6781 aaggtaatat tattgcaaaa ggtatttatg atagtacaac aaacacaaca acatatactt
     6841 ttacgaacta tgtagatcaa tatacaaatg ttagaggtag ctttgaacaa gttgcatttg
     6901 cgaaacgtaa aaatgcaaca actgataaaa cagcttataa aatggaagta actttaggta
     6961 atgatacata tagcgaagaa atcattgtcg attatggtaa taaaaaagca caaccgctta
     7021 tttcaagtac aaactatatt aacaatgaag atttatcgcg taatatgact gcatatgtaa
     7081 atcaacctaa aaatacatat actaaacaaa cgtttgttac taatttaact ggatataaat
     7141 ttaatccaaa tgcaaaaaac ttcaaaattt acgaagtgac agatcaaaat caatttgtgg
     7201 atagtttcac ccctgatact tcaaaactta aagatgttac tgatcaattc gatgttattt
     7261 atagtaatga taataaaaca gctacagtcg atttaatgaa aggccaaaca agcagcaata
     7321 aacaatacat cattcaacaa gttgcttatc cagataatag ttcaacagat aatggaaaaa
     7381 ttgattatac tttagacact gacaaaacta aatatagttg gtcaaatagt tattcaaatg
     7441 tgaatggctc atcaactgct aatggcgacc aaaagaaata taatctaggt gactatgtat
     7501 gggaagatac aaataaagat ggtaaacaag atgccaatga aaaagggatt aaaggtgttt
     7561 atgtcattct taaagatagt aacggtaaag aattagatcg tacgacaaca gatgaaaatg
     7621 gtaaatatca gttcactggt ttaagcaatg gaacttatag tgtagagttt tcaacaccag
     7681 ccggttatac accgacaact gcaaatgtag gtacagatga tgctgtagat tctgatggac
     7741 taactacaac aggtgtcatt aaagacgctg acaacatgac attagatagt ggattctaca
     7801 aaacaccaaa atatagttta ggtgattatg tttggtacga cagtaataaa gatggtaaac
     7861 aagattcgac tgaaaaagga attaaaggtg ttaaagttac tttgcaaaac gaaaaaggcg
     7921 aagtaattgg tacaactgaa acagatgaaa atggtaaata ccgctttgat aatttagata
     7981 gtggtaaata caaagttatc tttgaaaaac ctgctggctt aactcaaaca ggtacaaata
     8041 caactgaaga tgataaagat gccgatggtg gcgaagttga tgtaacaatt acggatcatg
     8101 atgatttcac acttgataat ggctactacg aagaagaaac atcagatagc gactcagatt
     8161 ctgacagcga ttcagactca gatagcgact cagattcaga tagcgactca gattcagaca
     8221 gcgattcaga cagcgactca gactcagata gcgattcaga ttcagacagc gactcagact
     8281 cagacagcga ttcagactcg gatagcgact cagactcaga tagcgactca gattcggata
     8341 gcgactcaga ctcagatagc gattcagatt cagatagcga ttcggactca gacagtgatt
     8401 cagattcaga ctcagatagc gactcagatt ctgacagcga ttcagactca gacagcgact
     8461 cagactcaga cagtgattca gattcagaca gcgactcaga ttcagatagc gactcagact
     8521 cagatagcga ctcagactca gatagcgact cagactcgga tagcgattca gattcagaca
     8581 gcgactcaga ttcagatagc gattcggact cagacaacga ctcagattca gatagcgatt
     8641 cagattcaga tgcaggtaaa catactccgg ctaaaccaat gagtacggtt aaagatcagc
     8701 ataaaacagc taaagcatta ccagaaacag gtagtgaaaa taataattca aataatggca
     8761 cattattcgg tggattattc gcggcattag gatcattatt gttattcggt cgtcgtaaaa
     8821 aacaaaataa ataatatgat taacttaacc aggtccatgt ggcctggttt tttctgttta
     8881 gaaattccca gatataaaac aaaaaatctt taaggaatag atacaattta atttgttaca
     8941 gaaatgtaat tgtctatgaa ttagaaaagc taagatatta atttcaaggg caaataatac
     9001 tattttttat gatgattaaa taaagattac aaaactcat
//
LOCUS       DANJFZ010000101         8986 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.101, whole genome shotgun sequence.
ACCESSION   DANJFZ010000101 DANJFZ010000000
VERSION     DANJFZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.101"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..615)
                     /locus_tag="PWN45_002146"
     CDS             complement(<1..615)
                     /locus_tag="PWN45_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDL0623892.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVI"
     gene            768..1187
                     /locus_tag="PWN45_002147"
     CDS             768..1187
                     /locus_tag="PWN45_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0623893.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            1214..1324
                     /locus_tag="PWN45_002148"
     CDS             1214..1324
                     /locus_tag="PWN45_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623894.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(1394..5164)
                     /locus_tag="PWN45_002149"
     CDS             complement(1394..5164)
                     /locus_tag="PWN45_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0623895.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(5392..5826)
                     /locus_tag="PWN45_002150"
     CDS             complement(5392..5826)
                     /locus_tag="PWN45_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623896.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5981..6451)
                     /locus_tag="PWN45_002151"
     CDS             complement(5981..6451)
                     /locus_tag="PWN45_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDL0623897.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(6499..7716)
                     /locus_tag="PWN45_002152"
     CDS             complement(6499..7716)
                     /locus_tag="PWN45_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDL0623898.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
     gene            8167..>8986
                     /gene="fmtA"
                     /locus_tag="PWN45_002153"
     CDS             8167..>8986
                     /gene="fmtA"
                     /locus_tag="PWN45_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDL0623899.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSF"
BASE COUNT         2853 a         1374 c         1468 g         3291 t
ORIGIN      
        1 aatcacgtaa acgataaaaa acaaaataaa ggtaatgata gatactggta ttagtttttt
       61 atttttaaaa atagccgtat gttttttggt gaaaatgtaa cctagataaa atattgggaa
      121 aaatacgatt gtccttgaaa tgcttaagta gctatcgatg ttatctgaaa aacctgctcc
      181 aatagatata ataattgaaa ctgatagcac tttatatgga ttaaatcttc taactattac
      241 taaaatgaca tgaaagaaaa atagcgtgat caaaaaccat aacgcaaata ctgggttaaa
      301 aggatcaagt tgtaattcgt cacttttacc tgttaagaaa taataaattg aaaagaatgc
      361 aaaaaatatc atataaggta ctatcaaacg ttttgaaatt ttttctaaat agtatggttt
      421 atcaatattt ttcgcgaaat aaccagatat aaacaaaaat gttggcatat ggaaactata
      481 aataactagg tataatgctg ataaatattt atcgccacta gtgtagggtt gtaacatatg
      541 tccaaatacg actaataata ttaaaattgc tcttgcgttg tcaaaaaaat aatctctctc
      601 ttttaatgaa gtcatatttt atctccttta tgtccataag actcttacta atttattata
      661 ttttgtatta gttatgcaac aaatttgtct attgtgtcga atagtattgt aaaaattata
      721 aaaaaagcat ataatattat aaataatatg tagtggagta gaacaacatg tataaacaac
      781 ttgaaaaact tattacactg actaacaatg acttaaactt agtgaataga agatttggac
      841 aacgcacgga tatcaaatct gaacagctag aacttctccg tattttattt aattacgatc
      901 gcttatcaca gtatgatttg acgatgaaga ttagcaggga acaatctata gtttcaaggt
      961 ggattaagaa attagttttg aaagggtaca tcacaagtca acaatctagc gaagatttaa
     1021 gatgtaaaga attaattttg actgatcaag cacgtacatt aatttcacaa ataaataatg
     1081 cacgttgcga attgattgaa gcaagatgtc aatgtttatc ggaagtcgaa ttagacaatt
     1141 taaatcaatt acttgataag ttaaatcaac gacgcatatc gttgtaacac gacaatagca
     1201 aacacataat ttagtgtcgt tgtgcacaca acaaaaattg aatcatgaat tacaagcaaa
     1261 agtagcggtg attgttaaaa ttgatggtaa acaatcaccg ctatttttgc ttgtgtatgt
     1321 ataaaaaagg gatcaaaggt catcccccat gattgatagt gggggatgac ttttgatcct
     1381 atgttcatgt tgcttattta tattgtggga tgtcgaagta tttgccgact tcgccaattt
     1441 tatcatagta gcctttgatg attttagcat tgatgttagc ccaatctaca tctgtagcat
     1501 attggtgtgt tcctggatgt gcaggattcc atctcatttt gtaaagtgta ttttgaccag
     1561 cttttacata tgagttgccg atgaatttag caccaccaac gattgctttt gatactgtgt
     1621 cccaaccagc ttgtttagca tatttaatac cttcacgtaa aggatcgtta tcatatgcag
     1681 caataccaaa tacgttatgg tatttcgtgt ttgagttagt tacaactttg ttgttcacta
     1741 catctgcacc tttcgctaat tgagaagtac cgttacctgt ttctaatagg gcatgtgaga
     1801 taagataaac ttcattaatg ccatacattt gagcagcttt gttaaatgca gcaccttggt
     1861 tttctaatac acctttacct tttaagaatt gattaatttt atcaatagaa atattttgtg
     1921 gttggtctaa gcgtaagaat tgatatttta atgctggatc ttgagctaaa cgcttcgtat
     1981 ccattgcatg cttaacatca ttaaagttag catctgtcca cttacctggt acacgttgta
     2041 cttgtggttt atattgtaaa ccagcttgta tttgagcaac ttggtttaat gtcatacctg
     2101 tttgattata cttaattaat tctttagcta aatcagttga tttaatccat gctaatttac
     2161 cgttagataa tttaccatag taccaagttt gtccattaat gacttgttct ttaacaactg
     2221 cgaatggttg ttcattaaat gcttttaatg agtatttagc tgtatcagaa tttggtgtta
     2281 catagtaata accattacca tttttaatta cataagtgta gttataatct ttggcagctg
     2341 atgtagttgg tttcacagcg gttggtgcag ttaaatcttt tgcatttacc caaccagtgc
     2401 ggttattaat agtaccgtat aaataaacat ctttgcctac agatacttgt ttcgttgcat
     2461 taaatgtacc ttgagcaatg ttattgcctg ttaaaatgac ttggttttta gtaccccaag
     2521 gaaccattga taagccgtta tttgatttat taacagtata tttttgagtc gttttaactt
     2581 ctttgcctaa gttttgaaca tttaagtctt ttacattgaa ccaacctaat gggatgttat
     2641 ggcttgtatt gtttaataat acatacgttt cattaccatg agcacgctct tttgttacat
     2701 agaacgtacg gtctgcatat ttcgcaccgt ttttcgctgt tttttcataa acagaagcac
     2761 gaataccagt gttgtttggt ttaacttgag caatcttgct aactgtttga gtcgtttgtg
     2821 gtttagtaac agtataagct tttacagctg tttttggttg tgctactgct tttttaggtg
     2881 cagcaggtac agctaaatat gctttactta cccaaccaga tttaccattt acagttccaa
     2941 ataaatagat agatttatca atttgttgtt gcttagtcgc tttaaaagtt tggttacctg
     3001 taccagaaac tgcaccagct tcttgtttat aagtgcccca aggtactgaa tataatttag
     3061 tgcctggttt tactgtatat gtttgcatta catttacagg tgattttgca ttgttataaa
     3121 taacgtcacc ttgtttaacc caaccaatta aagttggact attgtaatct ttaactaagt
     3181 agaatttgtt tccacctaaa cttgcttctt ttgttacagc aaatgttttt tgaacttctt
     3241 tcgttggctt accagttttg tcataaactg tagtgaataa gccattgttt ttagcattaa
     3301 tttgagcaac accgtttaat gatgaaactg ttaatttatt atttgttgta ggtgttgatg
     3361 gcttaggtgt tggtgtaggc gtaggtttag cagtatcaac taaatatgct ttacttaccc
     3421 aaccagattt accattcaca gagccatata aataaattga tttatcaatt tgttgttgct
     3481 ttgaagcctt aaatgtttgg tttccagagc cagacacact accagcaact tgtttagatg
     3541 taccccaagg tactgtataa agtttcgtac caggtttgat tgaatatgat tgatttacat
     3601 ttacaggtga tttagctgtg ttgtaaacca catcgccttc tttaacccaa ccaaatttat
     3661 taccagaatt gtaatcttga acaagataga atttttgatt acctaatgta gctgttttag
     3721 atacagcaaa tgttttttga acttcattag ttgctttacc agttttgtcg tatacagtag
     3781 tatataaacc actatttgtt ggtttgattt gtgcgacacc attgtttgca gcaactgtta
     3841 atttaccagt tgatggtttc gacggtgttg ttggttttga tggtgtagta ggggtagttg
     3901 tagattgcgt accccatggc gccactttac ccatttttat taaatatttt tcattaatta
     3961 agtcatataa ttgatcataa ctataattat gacttcttaa atatccatgt ggatcggcat
     4021 ggtcagtacc acctaaatat ttacttacag cgtagtgagt ccatactgta ccatttccat
     4081 catactcagc actgtctggt tttaaaccat aatattgtaa ttgtgtagct gcatagtcag
     4141 catagttatt cattgaacgt gcaaatgaag catagtcgtg tgtgtgtacg atttcaacat
     4201 tgatgaatct agggttaccg actgcaccga caccccaaga taagtaatcc gttggtgctg
     4261 tttcgattat acgatcccca tcaacaaatg catgtacgaa tgcgttttga tagttatttt
     4321 tcatataact aatttcacca tttatcgtcg aacgatcatt agctgtatca tgaactacga
     4381 taccttcagg acgacctacg ccgttacggt atgcgtattt agggaagtaa gatgtataat
     4441 cttcttcaat tttaggtgct tttaagttat ttttacgaat gtaatcgtta attgaagagt
     4501 ttacttgtgg tttatatttt ggcaaactcg tttttggtgt tgcagcaact gatcttggtt
     4561 gtgctgaagc gctaaaagta gttactttag gtgtcgcttc agttttagct ttaggtgctg
     4621 atgtagttgc agctttaggt gctgcggttt tatattgcgt ttcaagagct gcaggtttag
     4681 cagctgattt aattaattct ggattaattt gattttctga attatcatct tcatcatcaa
     4741 ctaaactata accagcattt gtaacattag tgttagtttt aggtgctgta gtgcttgttg
     4801 actttgcaac aggctgcgta ttatttgtag tcgctgattg attagcacga gtgtcaccat
     4861 ttacttgtgc agtatcaact ttttgactta cttgagcatt gcctgttttg ttatttgctg
     4921 tttttggttg gacaatagca gggtcttgat atacttgagt gccagaaatg ttttgcgttg
     4981 gattttttac ctcagctttt gcttgttcag tagttgcttt aactttatta ctatctaaaa
     5041 cgtttttatt agtagtttga tcttgtgtcg tctcagctgc ttgaacttga tgtgcagtga
     5101 ctgctgaacc tacaagcgtt aatgcaacca ttgatggtag tttgtaattg aattttttcg
     5161 ccattctatt tattactcct aacatttatt aattattact aacattatag tacctgtttt
     5221 atatacctgt gcgtatttaa ccaatttgta atatactctt aatttacatt ttcactgtaa
     5281 aaaaatgaaa aaaattttga atctcaatgg tgacgtgaca aatcaaatag aattgatgtc
     5341 aatttaagaa ttggtttatt acaaatgttt ctaaaataga tacaactttt tttaattaag
     5401 cgaggttaac ttttttgtca tttcaatatg ctcgatgcct tcctcaagaa atatattacc
     5461 tctcatttta aagtttaaac tttcataaaa tgggatagca tgacattggg cattcatagt
     5521 agctacgtaa aaaccttcat ctttagctaa tgattctaca gcttgcataa gcattctacc
     5581 cattccttgt ccacgatgtg atttcatcac agctactcgt tctattttga cagttgtttc
     5641 attaataggg cgtattcgag cagtggcaac tggctgtcca ttatcatatc caatgaggtg
     5701 aatagattca gattcatatt catcaatttc actttcctca gggacgcctt gttcttctac
     5761 aaacactttc tttcttatat agaagcaatc ttctaacatc ttttgattgt ttacttttga
     5821 aaacataaaa ttcctccaca tcctaacaaa atcatttaca tttaatttac taataataac
     5881 atagaataac gattgttatt ttcctcaaaa ataattagac ataaaaaagc tataaagatg
     5941 attgttgtga atcatcttta tagctaatta attattaatg ttatagtgct tcttttctaa
     6001 taattgctgt atattgccaa aagattcgca tttgagcaat attccaatta ttcattccca
     6061 ttgtaaatcc tgaaggctta aatatattca catcagttac ttctaaagct gctgcaacta
     6121 aatattgtaa tggaatgcta agtgatttca ttgtttcggt aatgccattt tcatcatata
     6181 cccatgaaaa tggtaactct gattcgaaaa catcaacact attgaaaatt tcaggtaggg
     6241 ctaaaatata acatgcacca tctacaatag gattgtcgat actatttttg taataaatta
     6301 ggagtgcttc atagttttct cgatgctcat gattgacgta aaaaaattca cttctaaaat
     6361 gagccatatc ttggctatga atgatttgtt gttctaacat attaaacatg ccatttatat
     6421 ttgcaatctt ttcgtatgtt tttctactca tgtatgatgc tcctttcata aatagtaaat
     6481 gttaatcgtc atataatatt aatttacaac accattttgg ttatttgaag cttgtggcgc
     6541 ttgttgtgtg ccaccttgat tttgatttga gttttgatct gtagcaggtt gttgttgatt
     6601 gctggaatca ctgttattag ttgaatcact gttctcgtta gatgtcttat ctttatctgt
     6661 cgtatcattt tgttcttttc tcaataaact attatctaaa ggcgttaatg gtattaatga
     6721 accataatga ttaatgacac gttgatctaa gaaatcattt ttatcattaa taggtgataa
     6781 ttctaagtct ttacgaagta agtttgcata tttttgaatg ctttcaacac ttggatgata
     6841 atagtaaata ccatttaaca tatcatcttt accttttaat tgcgcagttt taatttcaac
     6901 atcatttgtt aagtacattt ttgctaaagc tttaatttca gagttagtta aattatgctt
     6961 tgcattttta cctacaattt gaatcacgtt atcaagttta tcaatagaat caacttcctg
     7021 tgctttttgg aataaaatct taattaattc catttgacgt tgtccacgtt ttaagtctga
     7081 atcatgatgt ctagttctag caactgctaa agcctcatca ccatttaatt tttggtaccc
     7141 ttttttaatt ttaatcttac cagtatcatc tgtgttaggt tcatttaagt cgtatggcac
     7201 atcatagtat atgccaccaa gctcatttac agcctcgaca aatgctttca tattgactct
     7261 cacataataa tcaacaggta cattcatggt agcttctacc gaatccattg cggcaattgg
     7321 accaccatat gcatgtgcat gggtaatctt atcgtaatag ccaactttag gaatgtagct
     7381 gatagtatca cgtggaatac taagcattct aatttgatgt tttgattgat taaaagtagt
     7441 taaaatcata gcgtctgatc tagagtgttc agcatcctgt cctttttttc ttcttccatc
     7501 gttatcatcg atacctaaga aaagaataga gataggttgt tcttcgggat tgactttatt
     7561 atctcttaag ttggattgac gattagcatt tttgctgtct tgagaagatt cgaatgcatc
     7621 ttgggacgtt ttaaaaagta acgtagcgaa gactattgga acaacaatga gaaccaatgc
     7681 tagaaggatc aaaaagtatt ttaaaaattt attcatgatt gatgctccta tattaaattt
     7741 ttgttaaatc ataatcataa ttttaaaata taattagttg aacgatattt atacaacttc
     7801 attactaata cattatagcc ttaaattgta gtactctata gtaaaaaata caaatgttat
     7861 gtcaaagttc cttgtaattt gaatgttttg ttaaaatcaa aaagcatttt taaaaatata
     7921 atgcgacttt agtatgatgt cttaaaaaat ttaaaggcgt aatataaatt cgagtgattt
     7981 atacaaagtg ataaggtata attaattttg ttgtatagat aacatattag aaaaacttat
     8041 tactatgtag cgtaaagcat aaatgaatga taagttaatt tttgtaagtt gtattattag
     8101 gcatctaggt tcaaattttt aacgatttaa atatataaaa tgaatacatt gtgaggcaat
     8161 tatcaaatga aatttaataa agtaaaacta gttatacatg cgtgtgtact attatttatc
     8221 attatttcta tagcgttaat ttttcatcga ttacagacga agacacattc tatagaccca
     8281 atacataagg aaacaaaatt atcagacaat gaaaaatatt tagtggatcg taataaggaa
     8341 aaggttgcgc cgtctaaact aaaagaggta tataatagca aggatcctaa atataagaaa
     8401 attgacaagt atttacaaag ttcattattt aacggttcag tagctatata tgaaaatggc
     8461 aaattgaaaa tgagtaaagg ttatggatat caagattttg aaaaaggtat taaaaacaca
     8521 ccgaatacga tgtttttaat aggttcagct caaaaatttt caacagggtt actgttaaaa
     8581 cagttagaag aagaacataa aataaatatc aatgatccag taagtaaata ccttccatgg
     8641 tttaaaacat ctaagcctat cccattgaaa gatttaatgt tgcatcaaag tggattatat
     8701 aaatataaat cctcaaaaga ttataaaaat ttagatcaag cagttaaagc gattcaaaaa
     8761 cgtggtattg atcctaagaa atacaaaaag catatgtata acgatgggaa ttatttagta
     8821 cttgcgaaag taattgaaga agttacaggt aaatcttatg ctgagaatta ttatacaaaa
     8881 ataggagatc ctttaaaact tcagcacaca gcattttatg atgaacaacc ttttaaaaaa
     8941 tatctagcaa aaggttatgc ttataatagt acaggacttt cattct
//
LOCUS       DANJFZ010000102         8977 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.102, whole genome shotgun sequence.
ACCESSION   DANJFZ010000102 DANJFZ010000000
VERSION     DANJFZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.102"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            93..1226
                     /locus_tag="PWN45_002154"
     CDS             93..1226
                     /locus_tag="PWN45_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDL0623900.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKMAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            1294..2700
                     /gene="gndA"
                     /locus_tag="PWN45_002155"
     CDS             1294..2700
                     /gene="gndA"
                     /locus_tag="PWN45_002155"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL0623901.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2919..3290
                     /locus_tag="PWN45_002156"
     CDS             2919..3290
                     /locus_tag="PWN45_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0623902.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            3229..3483
                     /locus_tag="PWN45_002157"
     CDS             3229..3483
                     /locus_tag="PWN45_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623903.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            3484..4503
                     /locus_tag="PWN45_002158"
     CDS             3484..4503
                     /locus_tag="PWN45_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0623904.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            4519..6168
                     /locus_tag="PWN45_002159"
     CDS             4519..6168
                     /locus_tag="PWN45_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0623905.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(6251..7117)
                     /locus_tag="PWN45_002160"
     CDS             complement(6251..7117)
                     /locus_tag="PWN45_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0623906.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7347..8831)
                     /locus_tag="PWN45_002161"
     CDS             complement(7347..8831)
                     /locus_tag="PWN45_002161"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL0623907.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         3102 a         1316 c         1667 g         2892 t
ORIGIN      
        1 taaatctgtg taacgtcaat tactgtaaca ttttatttca tcaattttta ccgtttggcg
       61 tacatgaaaa ttttgataaa aggggttcca ttatgattaa tgagcaaaga ttacttaata
      121 cgttttttga acttgtacaa atcgattctg agacagggaa tgaatcaaca atccaaccta
      181 ttttaaaaga aaaattcata gcattagggt tagatgttaa agaagatgaa gcggctaaac
      241 atcctaaatt aggtgccaat aatctagtgt gtacaatgaa tagtactatc gaagaaggcg
      301 aagtacctaa attatatttg acgagccata tggatactgt tgtacccgca attaatgtaa
      361 agccaattgt aaaagatgac ggctacatat actctgatgg tacgactatt ctaggtgcag
      421 atgataaagc aggattagca gcgatgcttg aagttttgca agtgataaag gaacaacaaa
      481 tcccacatgg acaaattcaa tttgtgatta ctgttggaga agaatcaggt ttaattggtg
      541 ctaaagaatt aaattcagag ttgttggacg cggactttgg ttatgctatt gatgcaagtg
      601 ctgatgtcgg tactacggtt gtaggtgcac cgacgcaaat gttaatttca gctaaaattt
      661 ttggcaaaat ggctcatgca agtacgccaa aagagggtgt tagtgctatt aatattgcgg
      721 cgaaagcaat tagtcgaatg aaattaggac aagttgatga aataactact gccaatattg
      781 gtaagtttca tggcggttca gctacgaata ttgttgcaga tgaagtcatt ttagaagcag
      841 aggcacgttc tcacgatcca gaaagaataa aaacacaagt caaacatatg actgatgtgt
      901 ttgaaactac tgcatcagaa ttaggtggga aagcagtagt tacagtggag caaagttatc
      961 caggatttaa aattaatgat aatgaagcgg ttgttaaaat tgcacaagaa agtgctcgta
     1021 atttaggatt atctgcaaat acaattattt ctggtggcgg ttcagacggc agtattatta
     1081 atacatttgg tattccttca gtcattttag gagtgggtta tgaaaaaatc catacaacaa
     1141 atgagagaat gccgattaaa tcattgaact tgcttgcaag tcaagtgtta gaaataatca
     1201 aaatagttgc gcgacattcg aaatgattta gtgtagtgta tgtctatttt gtgatacaat
     1261 actaatgaaa aatcaacaag tgaggtaagt aatatgacac aacaaattgg agttattggt
     1321 ttagccgtta tgggtaaaaa cctagcttgg aatattgaat cacgcggata tagtgtatct
     1381 gtatttaacc gctcaagtga aaaaactgat ttaatggttg aagaatcaaa agggaaaaat
     1441 attcatccaa cgtattcatt ggaagaattt gttaattctt tagaaaaacc acgtaaaatt
     1501 ttattaatgg ttcaagcagg taaagctacg gacgcaacga ttgatagttt gttaccttta
     1561 ttagatgatg gagatatttt aattgatggt ggtaatacaa actatcaaga tactatcaga
     1621 cgtaataaag cattagcaca aagtgcaatc aactttattg gtatgggcgt ttctggtggt
     1681 gaaattggag cgttaactgg tccgtcatta atgccgggtg gacaagaaga agcttataac
     1741 aaagttgcag atattcttga tgcaattgct gcaaaggcaa aagacggtgc ttcatgtgtt
     1801 acttatattg gacctaatgg tgctggacac tatgtgaaaa tggtacacaa tggtatcgaa
     1861 tatgctgaca tgcaattaat tgcagaaagt tatgctatga tgaaagaatt attaggcatg
     1921 tctcatgaag acattgctca aacgttcaaa gactggaatg ctggtgaatt agaaagttat
     1981 ttaattgaaa tcactggtga tattttcatg aaattagatg aaaataaaga agcattagtt
     2041 gaaaaaattc ttgatactgc aggtcaaaaa ggtacaggaa aatggacttc aattaacgca
     2101 ttagaattag gtattccatt aacaatcatt actgaatctg tatttgcacg ttttatctct
     2161 tcaatcaaag aagagcgcgt aaatgcttct aaagaattaa atggtccaaa agcatcattt
     2221 gatggtgaca agaaagactt tttagaaaaa attcgtaaag cattatatat gagtaaaatt
     2281 tgttcttatg cacaaggttt cgcgcaaatg cgtaaagcaa gtgaagataa tgaatggaat
     2341 ttaaaacttg gtgatttagc tatgatttgg agagaaggtt gcattatccg tgcacaattc
     2401 ttacaaaaga tcaaagatgc ttacgataat aatccaggat tacaaaactt attgctagat
     2461 ccttacttta aaaacatcgt tactgaatat caagatgcgt tacgtgatgt tgtcgcaact
     2521 ggtgttcaaa atggtgtacc aacacctggt ttctcatcaa gtattaatta ctatgatagt
     2581 taccgtgctg ctgatttacc tgcaaactta attcaagcac aacgtgacta cttcggtgct
     2641 catacgtacg aaagaaaaga taaagaaggc gtattccata cacaatggat tgaagaataa
     2701 tattgattaa aggatagttg aaagatacta tcctttttta tatcttttga taaatataaa
     2761 agagagtgtg atagagtagc tacaacctat aaaaaattac acaagtatta tccttgacct
     2821 taaaagcatc ccgccatatt tttaagcatt ttgtgaagta ataaaatatt tactgtgtta
     2881 tgatagcatg tactatagag aaatagaggt actattttat ggaaaagtta aatcaagtta
     2941 tgttatatgt tgatgatgtc gaaaaagcga aagcattttg gacagaaact ttagaatttg
     3001 ttgttgtaag tgaaacaccg ttagctgagg actatgttgc agttgaagta tcacctacca
     3061 aagatgctga aacgtcattg acgattatgg caaaagaatt tattgaaaag catagcccgg
     3121 aagtgaacct aggaacacca tctttaatgt ttaaagagaa aaactttgat gcattatact
     3181 caaaattaaa tgatttaggt ttaactgggc atgatatagt agaaatgaat ggtcaacgtg
     3241 tatttaattt tcaagatggc caaggtaact attttgcagt tagtgactaa acaatcgatt
     3301 ttaatattta tacaaacctt tatattgatt ttcggattga atttcagtat gaaggtttta
     3361 tttttatcgt tttatgcaat cgtttgcaca aatcttttga cttttttaga aagtgtgact
     3421 aaactggtat tatacagtgg ttggaagcgt tttccaatgc agaaattaac aggaggtgat
     3481 taaatggtta cgattaaaga tgttgcacta aaagccggtg tttctccttc gacagtttca
     3541 agagttataa aaggaaatca acgtattagc gaagcgacaa tttcaaaagt gaagaaagtt
     3601 atggaagaat tgaattattt tcctaatacc gctgctagaa ctttaattac aaaccaaaca
     3661 tataaaattg gtttagtgtt aaaagggtct gaggagccta ttcgactgaa tccattctac
     3721 atcaatgtat tgctaggaat ttctgaaacg tgtaaccagc atggctatgg tacacaaacg
     3781 acagtctcaa ataatatgaa tgatttaatg gatgaagttt ataaaatgat taaacaacga
     3841 atggttgatg cgtttatact gctctattca aaagaaaatg atccgattaa acaaatgtta
     3901 attgatgaaa gcatgccatt tattgtgatt ggtaagccta catcggatat agatcatcaa
     3961 tttacacata tagataatga taatatatta gcttctgaaa atttgacacg acatgttatt
     4021 gaacaaggtg tagatgaatt aatatttatt acagaaaaag gaaattttga agtttcaaaa
     4081 gatagaattc aaggatttga aacgatagct tctcaaaata aaattaatta tcaaattatt
     4141 gagactagta atgaaagaga agttattttt aattacatgc aaaatctaca tacgcgtttg
     4201 aaggacccaa atattaaaca ggcaatcatt tcgttagatg ctatgttaca tttagcgatt
     4261 ttaagtgtcc tatatgaact taatattgaa attccgaaag atgtaatgac agcaacgttc
     4321 aatgattctt atttaaccga aattgcgtca ccaccacaaa cctgtattga catcaagcct
     4381 cgaatgttag gtcaacaggc tggtgcagca attttaaata tattgaaaaa caaagcacag
     4441 gatgttattg aactcgtcat tatagataca gaattaaaaa taagaaaatc aacacagcga
     4501 tagcaaagga gttttaaaat gaataagcaa tggtggaaag aagcagtagc atatcaagta
     4561 tatccaagaa gttttaatga tagtaatcac gatggtattg gggatttacc tggaatgatt
     4621 gataaattgg actacttaaa agatttcggt atcgatgtca tttggctcag tccaatgttt
     4681 aaatcaccta atgatgacaa tggttatgat attagtgact accaagagat tatggatgaa
     4741 tttggaacga tggaagactt tgatcgttta ttaaaaggtg ttcatgatag aggcatgaag
     4801 cttattttag atttagttgt aaatcataca tctgatgaac atccttggtt tatagaatcc
     4861 aaatctagta aagacaatcc caaacgtgat tggtacattt ggcaagatcc aaagccagat
     4921 ggctctgaac ctaacaactg ggaaagtata tttaatggat ctacatggga atatgatgct
     4981 aatactgagc aatattattt ccatttattc agtaaaaaac aacctgattt gaattggggt
     5041 aatccggaag ttagagatgc tgtatttgaa atgatgaact ggtggtttga taaaggcatt
     5101 gatggattta gagtagatgc aattacgcat attaagaaga cgtttgaagc gggtgactta
     5161 cctgtacctg agggtaaaac atatgcccca gcatttgatg tagatatgaa tcagccaggt
     5221 atacaaactt ggttacaaga gatgaaagat cgctcattaa gtaagtatga cattatgact
     5281 gttggtgaag cgaatggtgt aagccctgat gatgctgatg actgggtcgg ggaagaaaat
     5341 ggtaaattta atatgatatt ccaatttgaa catttgggac tgtggaatag tggtgattct
     5401 cactttgatg taaattcgta taaatctgta ttaaatagat ggcaaaaaca acttgaaaat
     5461 aaaggttgga atgcgttgtt tattgaaaat cacgaccaac cacgacgtgt atcgacgtgg
     5521 ggtgacgatg acaagtattg gtatgaatca gcaacaagtc atgcagctgt ttatttcttg
     5581 caacaaggta cgccattcat ttatcaaggt caagaaattg gtatgacgaa ttatccattt
     5641 gaaagtattg aaacgtttaa cgatgttgct gttaaaaatg actatcaaat agtgaaagct
     5701 caaggtggag atgtagacgc tttacttgcg aaatataaag atgagaaccg agataattct
     5761 cgcacaccaa tgcaatggga tgatacgtta aatggaggat ttacaaatgg tgaaccgtgg
     5821 ttcccagtga atccgaatta taaaactatc aatgttgcac aacaattaga agatgagcat
     5881 tcagtattac aattttataa agatttaatt caattaagaa agtctaatga tgtatacgta
     5941 tatggtcaat ttgatttagt agatgctgaa aattcacaag tttttgcgta cacgagaaca
     6001 ttaaatgaaa agcaagttct tattgtaggt aatcttacta accacgaagc agaattaact
     6061 gtaccatttg atttaagcca tggagaagta aagctattta attatgatgc caaagttaat
     6121 ttaaaacagt tacgtccata tgaagcatgt gttatcgaac taaattaaca tattaatatg
     6181 ccccctaata gaatctaagg ccatttgtag acctatagat atattagggg gcttctttgt
     6241 tttttattat ttaatcttcg tcataaggac tctgtttaac aggaatccaa agctgaattt
     6301 tagtgaatgg gtcattaaat gaaatatcga gagggtacac ttcaacatat aaatcatttc
     6361 gttcatatgg caatgttaac tgcaaacttg attcaatata gtaccaagct tcattagttg
     6421 cataatcaat ttcaccttgt aaattgaatt tcgcacaatg tttgccaggt aaaaatcgac
     6481 tttctaagtg tgcaggataa cgttcacttg gtacacctac aaatatttct aaaccatttt
     6541 caagaggaca actaataaca aatagttcaa atggactaac gtcattatat cgtcgtaact
     6601 ctttaatttt accatcaatg agcaagtctt ctaaaaaatc cggaacatta aaaggatgtg
     6661 acaaatactt agtgtctata aatcgtgcat atccaaccaa tgaaatatca tctgtctctt
     6721 ctaatctgta tggataaggt gctctctcag tagttgataa tttgatatat aatcgctctt
     6781 gaatttgtaa ttcatctttt ttagtagagg cttgaatagg tgatacgccg tgaaaatcac
     6841 taaaatcatt tgcaaaatca tttgaatttg catagtgata ttttttagcg atatctacaa
     6901 gtcgtgtagc accattaatc acatcatttg cagcgagtgt cattttacgc gcgcgtgcat
     6961 aagcttctgg agataagccg acaatcattt taaatgattg atcaagatgg tatggcgaaa
     7021 gaccaacgta atcacttaat tcttgcaaat tgaaaggctc taataaacga tcttcaatat
     7081 aaacaattgc ctgttgtatt tgcttgataa cgtccaaaac tttcactcca attaattcaa
     7141 tcatttattt ttattctaca ttatttctat aaattataca cccatttgtt caatgattat
     7201 taaaatagtt ttgggcattg taaaatataa tttcataata tagtctagaa aaaaagcgaa
     7261 tgatagaaca attgatttac ttgattcgta atcaatcctt gtcattcgct catttatttt
     7321 tgtttaacat gtgcgtttta attcaattat tgaatatcgt cccaccaatg gttaccatca
     7381 cgagcaagta gtaaatcact ttctaatgga ccattagtac ctgattcata gttagggaat
     7441 tctggatcaa ccatattcca ttcatcttga attgcatcaa caaatttcca tgttgatttt
     7501 aattcttccc agtgcgtgaa gttagtggca tcacctttaa gacaatcaaa taatagattt
     7561 tcatatgcat ctacagtatt cattttatct tgagcgctca ttgagtaaga caattggaca
     7621 ggttctgttt cgataccttg tgtatttttc ttagcattta gatgtaaaga tacaccttca
     7681 ttaggttgga tattgattac taataggttt gaatctaaca gtttatcagt ttcatagtat
     7741 aagttcattg gtacttcttt aaattcaacg acaacttgaa ttgttttaga tttcatacgt
     7801 ttaccagtac gaatatagaa tggtacacca gcccatctaa agttatcaat tgttaattta
     7861 cctgaaacaa aggtaggtgt gttagagtca tctgcaacgc gatcttcatc acggtatgct
     7921 ttaacttgtt taccatcgat atagccttcg ccatattgac cacgaacaaa gttcttttta
     7981 acatcttcag attggaaatg acgcagtgat ttaagtactt ttactttctc agcacggata
     8041 tcttcactat ttaaactaat aggtgcttcc atagctaata atgcaaccat ttgtaacatg
     8101 tggttttgca ccatatcttt tagcgcgcca cttgattcat aataaccacc acgatcttca
     8161 acacctagta tttcagaaga tgtaacttgg atgtttgaaa tatatttgtt attccataat
     8221 ggttcaaaca tcgcattcgc aaaacgtaat acctcgatat tttgaaccat gtcttttcct
     8281 aaatagtggt caatacgata aatttcttct tctttaaatg atttacgaat ttgattgttt
     8341 aatgcttcgg ctgattttaa atcactaccg aatggttttt cgataacaag gcgtttaaat
     8401 ccttttgtat cagtaagacc agaagatttt agataatcag aaataacgcc aaagaattgt
     8461 ggtgccattg ctaaatagaa tagtcgatta ccttttaatt caaattggct atctaattca
     8521 ttactaaaat ctagtaattc ttgatagctt tcttcattac taacatcatg tctatgatag
     8581 aagacatgtt ccataaacgc gtcaattttg tttgtatctt ttacgtgctt ttgaattgat
     8641 gattttactt gattacggaa atcatcatta gtaatgtcac gacgtccaat accgatgatg
     8701 gcaatatgtt catctaaatt gtcttgttgg tagagatgga atattgatgg aaacaactta
     8761 cgatggctta agtcaccagt tgcaccaaag attgtgatta aacatgggat gtgtttgttt
     8821 ttagtactca agattaaaac ctcaattctt aattagatat atgatttatt attataagat
     8881 aatccattca gtaggtcata caatatgctc gactgtatgc agtgtcttaa atgaaatatc
     8941 gattcatgta tcatgtttaa tgtgatactt attaatg
//
LOCUS       DANJFZ010000103         8886 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.103, whole genome shotgun sequence.
ACCESSION   DANJFZ010000103 DANJFZ010000000
VERSION     DANJFZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.103"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(224..1390)
                     /gene="norA"
                     /locus_tag="PWN45_002162"
     CDS             complement(224..1390)
                     /gene="norA"
                     /locus_tag="PWN45_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDL0623908.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1599..2021
                     /locus_tag="PWN45_002163"
     CDS             1599..2021
                     /locus_tag="PWN45_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0623909.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            2207..2824
                     /locus_tag="PWN45_002164"
     CDS             2207..2824
                     /locus_tag="PWN45_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDL0623910.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            2821..3105
                     /locus_tag="PWN45_002165"
     CDS             2821..3105
                     /locus_tag="PWN45_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623911.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(3260..4633)
                     /locus_tag="PWN45_002166"
     CDS             complement(3260..4633)
                     /locus_tag="PWN45_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDL0623912.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(4719..6272)
                     /locus_tag="PWN45_002167"
     CDS             complement(4719..6272)
                     /locus_tag="PWN45_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL0623913.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(6555..6842)
                     /locus_tag="PWN45_002168"
     CDS             complement(6555..6842)
                     /locus_tag="PWN45_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623914.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(6877..7785)
                     /locus_tag="PWN45_002169"
     CDS             complement(6877..7785)
                     /locus_tag="PWN45_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDL0623915.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(7888..8814)
                     /locus_tag="PWN45_002170"
     CDS             complement(7888..8814)
                     /locus_tag="PWN45_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0623916.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT         3043 a         1574 c         1235 g         3034 t
ORIGIN      
        1 ggattgcaat ttaaaaagaa tgtaatattt gtaacatgtt tattactcaa aatcattttt
       61 tgttacatat aacatttaaa tataaagagt aaaacattta gtcaatcgaa tctttttaaa
      121 tgtaaagcat cgaattttct taataaaatt cataagaaaa acgatgctaa tcattcataa
      181 tattacgctt tactttatat caatatacac caaaatactt atgctacata ttttgttctt
      241 tcaattttgc tctatgttgc ttttcaatta aaacaataac aacacctgct aatgaaacac
      301 ctatagccat ataaattggt gcttcaatgt gtacatcaaa taacgcacct gcgattaaag
      361 gacctatgaa attacccata ctagtgaatg tcgagttcaa tccgcctgca aagccttgcc
      421 tttctccagc aatattagaa aaataatttg taatggctgg tcgtatcata tcaaaaccta
      481 tgaagacaac aaaactgatt aacattattg accaatagtc attagcaaaa actaataata
      541 ttaagacaac aactgaatat aataatgacc aagctataaa tgttaactct gagaaatact
      601 tcataaattt atcgaagaaa tagatttgga aaagtgcccc aaatataccg ccacccgtaa
      661 tagcaatcga aatatcttta ggtgaataat ttaccttgtc agctgtgtat agtgaataca
      721 atgtttcaaa tgcagataaa ccaaacgata atacaagtgt taaaataact ggtgtaataa
      781 acactttcca gttaattttc gttagcaatt gtggctctaa cttttggaaa ccacttgtcg
      841 tagacttttt cggatcgtga atcaatacaa ttgacattat aaatgctaga atacctaatg
      901 ctcctgcaaa gtaaaatggc atacgatgtg aaacttctgc cataaatcca ccaatccctg
      961 gtcctaaaat gaatccagaa ttgataatcg ctgacatgta gccaaagttt tttgcttttt
     1021 gatggcttgg tgaaatgtca gctattaaac ctgtcacacc aggcattacc ataccagcac
     1081 tcataccacc aatcactctc gataacatca ataccgaaaa attgtggcca actgcaaaca
     1141 taaattctga cactgaaaac aaaattaatc ctatacatat aattaatttc ttccctaatt
     1201 tgtcagctag cgtaccacca aacggcgata taatcatttg agataacgca aaagcagcaa
     1261 ctagtaatcc taaatcacta ccagttaatc ccaaatcttt taaataaaca ggcaagactg
     1321 gtattactaa accgatacct aaaaaaatca agaaaatatt aaaatataag acaaaaatct
     1381 gtttattcat atgctcacct cttttttctc catgtcatgc ttaaagctgt tattcttctt
     1441 ttccacgaca gattgcaatt catgctaaat attcgtaaaa agtttctata ttgtattaca
     1501 acattgctac atttgacaat attttttctt tgtaattatc actattttcc atttaattgt
     1561 ataattaatg acatattaaa ataaagggga ttgatcatat gaaattgcaa aaaagtaatc
     1621 atactatact actagtgttg gaaaaaggag aagatatcgt tgaatgtata acaacctttg
     1681 cagatgacca agacttaaca tttacatctg tcagcggcat tggtgcatgt gatgacgttg
     1741 tattaaaatt tttcaattta acaacgaaac aatacgaaga aaaacatatt actgagccac
     1801 tggaattgac gagcttatta ggtaatattt ctcgtctaga caatggtcat tttgcacatt
     1861 tgcatgctac tttcggtact caatcatatg aaacttttag cggacatctc gctaaagcaa
     1921 ttgtttctgc aactgctgaa atcattctta ctgtaactga tttggacatt caacgttcat
     1981 tcaaagatgc agtcggttta aatttacttg atcctcaata gcactttact ttttagttga
     2041 atcaaatttt attacaagtt atcataagtg cttaatcata aatgcattct cgtttattaa
     2101 tatacttcaa cgagttaaat taaattttta attaggaatt gaatgcgttt ataactatca
     2161 catttgaatg aataactttt tcattagaaa gtagtgttta ttattgatgc aatctcgata
     2221 catcgctaga atttactttt taattttatt tatcgtctcc ctttttgaga ctaaaatttt
     2281 tcaatttatg actttaaatt tgtttttagc atatatacct tttgagctta gtctattatt
     2341 acgattattc aaaccgaata aaaagtatga atggccactc tttatcatat atagttttat
     2401 atttgtgtta cttttaccaa atacatttta tatggtaact gatttaatcc atttgaacca
     2461 atttcaattc aacttttacg caggactaaa tttaagtgag tggaaatact ttacctattt
     2521 actgcttggt gtcttcttag cggtttatgt gatgatttta atctattttg aaatactaac
     2581 attgacttct caccgttggc ttaatagatt aattgttgtc gtgctaatgt ttttaaatgg
     2641 attcggaatt tatattggtc gtttcttacg cttacattcc gtttatttct ttaatgagcc
     2701 cttacatgtt ctaaatcaca ttctagaaag tctaacgctt aaaactgcta tgtttgtatg
     2761 ttttatggtt gtcatgcaag cagcaattct attatttgga aaaggagtgc gtttgaacaa
     2821 gtgacaataa atataatcgt tttaatagta tctatcattg tctttcaatt gattattgga
     2881 cacatatggc atgatattgg tttaagttat ttaagaagta tcttactcat gatgctgcca
     2941 tttggactag gtgtatttat acaacaagtg tcgtattacg aacgacaata tcctaaatgg
     3001 caagtgcctc aaaacataaa agttagattg aaatatattt atctcgctac gtttttagag
     3061 tatgtcgtac tatacctcac tttatttacg gacatactaa gataacatca aattaataag
     3121 aaaactaaat acaacttata tatttgagga aagccaattt ctatcacatc aataatagaa
     3181 attggcttat ttatattgct taagaacttt atattctgct ccagatttta ctcatgccag
     3241 atctatcaat tgagccgaat tagtctagcg ctttaaatgt agctaaaact tgtgtacgtt
     3301 tttcttgatg atctaccatt tgtcttggat aatgactacc taattcaatc ccctgttcaa
     3361 aaaggttgga ctcattgcgt tgtgtatcat gcaaatattt tgcatcaatt tgattaaaaa
     3421 tcggaagata tgttttgata tacaaagctt tagcatcaaa gcgttcactc tgtcttattg
     3481 gattaaacat tctaaaatac ggcactgcat ccgtacctgt agaagcagac cattgccatc
     3541 catgaatatt tgatgctgca tcatagtcaa taaggtgctt tctaaagaat ttttctcccc
     3601 atgtccaatc tataaataaa tctttggtta aaaattgcga cacaaccatt ctcattcgat
     3661 tatgcataaa accagtttgt gtcaattcca ttattgctgc atcaatgatt ggaaatcctg
     3721 tttgcccttc gcaccatgca ttaaaatccg cttcattttg cgaccatttt atctgtcgat
     3781 atttaggttt gaaagcttgg tatgaggttt caggatactg tgtcattaac acataataaa
     3841 attctctaaa aatgagttca cgtataaatg cttcatagtt tgcctcatct tcatcataac
     3901 gcgccaataa atcattaaaa atttcaataa tatctaataa tccatatgct aaagcaacac
     3961 ttagctgact tgttaatact tcaggtaaat agtctcttcc gctttcgtaa ttctgtatat
     4021 cttgatctaa aaaatgttgc catttattct gttcaattat ttgtgaggat ccaaatgcta
     4081 tgtcatcaaa cactaaatca tcaggtgatg caatgacaaa gtcttcgaat gattttaaat
     4141 catagtgata tacgtctcta tgcctcaagt aaggtcgcca ttttttatag aaactagtaa
     4201 aaacattgta tggctcccct tgttgattca ttgttttact cgctttaaag taatgattcc
     4261 cacgtatcaa agtaacggca atgcgtgctt cattgaataa agaacgttga tgtaaaatat
     4321 catattcttc aagatgataa ctcataatat cacccgcagt cactacttca gagatattgt
     4381 tttctttaca aaatgaaatg atatcgtcat aagacataac ataaggtgta agcttttctg
     4441 cgtgcaacga ttggtaaaag cgctgcaaag taccgcgata aaactgtgct ttcaaatcag
     4501 aagctgaaga catgtcctct tccggaatga taaaataaca tgcatcaatg tcttcttttt
     4561 gttgataaat atattcaaat aatggattgt gttccattcg aaacattcga tttaataaca
     4621 cagcaattgc cataaacaaa acacccttca attatctttt tattttatta agctttttgt
     4681 cactttaaaa taacctagat tcttatttat atttttaatt aaagcaaacc taaccatttc
     4741 caataagtca tgctaaaaac tatcactaga atatacccta caattgtcaa tggtatacct
     4801 gccttcaaga aatctttaac cgtaaaagta ccagtgccat aagccaacat attttgaggt
     4861 gcactaacag gtaataagaa accaaaacta ataacaaatt gttgaattaa aacaaatcct
     4921 atagactggt ctcctaagtg taacgtagag gttagcgaaa taaaaacagg tattaacgct
     4981 gatgataaac ttgttgcact cgcaaagccc aaatgaatca atatattaaa aagcgtgata
     5041 agcgcaattg tcgcgataat aggtaaatgt tttaaaccta aaacaccaaa agtttgatca
     5101 cttaaccatt gagctgcacc tgttttcaaa agaacgttac ctagtgaaat acctacacca
     5161 aacacgataa ttgttcccca tggtatttta ttttcaacat gtttccatgt catgacacca
     5221 attttcggca ttaacataac acctaaagca ataatagtaa tggatgcaga gtcaatcgga
     5281 tgtaatactt tttcagttga ccaaaacagt aataataaca tcgatataac aattaaacgc
     5341 cattcacgtg ggctaacggg gccaagttta tgcaattctt cttttattaa atctttacca
     5401 ccttctattg tattaatttc tggaggcatc actttaatca tgatgaaata taaagctacg
     5461 gacataacta tggaccaagg cgctgcatat aagaaccact cgccccatga aacatcaaat
     5521 cctaattgat ggtttataaa attaatcgct acgatatttt gtgctgccgc cgttttgata
     5581 ccaatattcc aaattgacac agcttgtact gaagtaatta ttaataaaga cgctaacttg
     5641 ctatctttgg aaactttaaa tgccgcaatc atacccagca agattggtac aactgcccct
     5701 gctctagctg ttgcagaagg aacgaaaaat gcaagtacaa ttgaaacgat aattgctcca
     5761 ataactatat ttctagtttt attaccaaca attgataaca ctaaaagagc tagtctttta
     5821 tgcaaattcg tttcttgcat agcagcagcc aaaaataatg cagctgcaac gagagctaca
     5881 gctgaagttg caaatccact aaacgctaat gataatgcat gattagttcc tagaaggtca
     5941 cttccagcta aaatagcact gccacttttc ggattaccta gcttctcccc taaattttga
     6001 acagggctaa atcctaaaag taatatcatt aagccaataa ttaaagttgc tgacaccgga
     6061 tatgatacag cttccgttac ccacataata actgcaaaag ctaaaatagc tagtactgcc
     6121 ttagccatta tcggcagact tgaaggtgcc gggagtaata gtacaactat aagtattata
     6181 aaactcatga caatccaaaa tggcttgtat tgcttttttg tcttttcttt cttttgttgt
     6241 tcatgttcaa ctacatgttt ttcttctgac ataacagtca cccctttgtt taatcttaat
     6301 ttaattataa caatttttta aaacatacat ttttaattta tgcaaaatac acaattttat
     6361 cgaaatattt tgtaaataat attgctaggt gatgagttat ctcggttaat tacaaaacga
     6421 tatctaagtt taaatattag atcataaaaa aactatctac tgaatatcct catagcatca
     6481 tttttctaag caaaagttaa acttagttag ataaaatgca aatgagatta ttgtagatag
     6541 tctcttttta atgtttaaaa atgattaatg ccataaacat aaataattcc aaagaatgcc
     6601 gcgccactaa gcactgttaa tataagactt agtaacaatg tgcgtttata atgtttaaca
     6661 aatgctatga acaaaatgac tacattatac gcaaagaaaa gtccgaataa tgtcattgtt
     6721 attttcaaat cagaatagaa aatatttaat agtaatacaa tgactgcata aattaaatac
     6781 ggaataacta taaatttgcg ttcaaatctt aattgttcga aacgtttatc ttcgtttgtc
     6841 atgatatgct cctttatgat ttaaaagtaa ataagtttac ggaatatctt gtacgataat
     6901 agcggtgtaa atgtcaaacc acgactgatc atctaaatta agttgtagcc cttcgattgc
     6961 ttgatcaata cgctttaact gacttgttcc aagtatcggc atgatacgat gcggtatttt
     7021 tactaaccac gctatcatca cagctgtgtc actcacacca tatttgtcag ctattgattg
     7081 aacaactttc ataatacgtt gcgctttaat atcttccttg tcgaaaattt taccgcctgc
     7141 aaaaggactc caagccatga tttgaacatg gttttgatac attgaatcca ttgttccatc
     7201 ttgtaaacta tcaacgtgat atggcgataa ttctaattga ttgatgctaa tatgtagtct
     7261 ttctttcata atatattgat ttaacaattg gtattgtgaa tgattaaaat tcgacacccc
     7321 gaatgacttc aacttacctt gtttaacaag tttagttaat gcatcagcaa cttgttctgg
     7381 atccatcaat ggtgaaggac gatgaatgag tagactatct aaataatcta cattcaaatt
     7441 gattaatgac tgttcaacag atttcacgat gtgcttacta ctcaaatcat aacgatgtcc
     7501 atttgtaaaa tcaaattgct tagaaggcaa aatgatacca catttcgtaa caatttgaat
     7561 tttatttctt aattcgggtg ataaatccaa agcattacca aacagtgatt cacattgata
     7621 atctccataa atatcagcat gatccatcgt tgtaattcca cgttcaacta attcatttat
     7681 aaaataattt aactctttcg cagtcatctt ccattcattt gcacgccaaa aaccttgtac
     7741 aagcctagaa aaatgaacat agtgattaat cattatttgt tccatatctc atccactcca
     7801 tccctcaatg tcacaaaata ttcacaataa aagtattaag aaattaatgt gctatttccg
     7861 ttaaattgaa acaacacatt ttctgattta cgtaaattgt agcatatcca attgattacg
     7921 tatgtgattt gtatctaaag tttcaccaat aataacaatc gttaatggta attgcatgcc
     7981 aattattcca tagtctggta aaccatatgc atattgaaat tcataaattg catttggttg
     8041 atctctaaat gacacataac ctttcaaacg tagaacagat tccggtaatt tcattataaa
     8101 ttgataaaac aaatgacgat caatcggacc tgtaaacgtg taagtcatac ttttaatccc
     8161 atgatgatgg tgatgatgag acgatatctc tctgtcttta acagttaagt ccaattcttc
     8221 caacgaaact tttccatagg ttgttttata agttggaata cttgcacaaa tcatacctaa
     8281 ttgcttatca attttctcaa gactgtcatc agttataaga tcaattttat taataataat
     8341 catgtcactt agtttcaact gatcttccat cagcgaaact gtattttcgg tatattgatg
     8401 acgttctaaa aatcgagtcg catctaatac accataaata atcggctttt caaagaaatt
     8461 aacgatttgc ggatcttgac atgcaactag taattccaaa ggatgcgcaa tgcctgtcgc
     8521 ttctatgatg acatgattaa cgtccccttt taaagcaatg gcttttagtt catagacaag
     8581 ttcttgttta agatcgcaac aaacacaacc attaatcaat gaatggactt caatttcatt
     8641 tgaaatacta ttgctatcaa tatcaaaagt accgaattca ttcatgatga ttttaatttt
     8701 ctcatcattt tttaataatt cactaatata atgtgtcagt aacgtggttt taccactacc
     8761 caaaaatccg ttaattatgg atattcttat tttttcatct ttattatttt tcattttata
     8821 ttctcttttc tattacagtt atttttaaaa atgataacat atatattaaa ttatcttttt
     8881 gcttga
//
LOCUS       DANJFZ010000104         8871 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.104, whole genome shotgun sequence.
ACCESSION   DANJFZ010000104 DANJFZ010000000
VERSION     DANJFZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.104"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            273..1370
                     /locus_tag="PWN45_002171"
     CDS             273..1370
                     /locus_tag="PWN45_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDL0623917.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1575..3137
                     /locus_tag="PWN45_002172"
     CDS             1575..3137
                     /locus_tag="PWN45_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL0623918.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3148..3255)
                     /locus_tag="PWN45_002173"
     CDS             complement(3148..3255)
                     /locus_tag="PWN45_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623919.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(3327..4121)
                     /locus_tag="PWN45_002174"
     CDS             complement(3327..4121)
                     /locus_tag="PWN45_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL0623920.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(4141..5217)
                     /locus_tag="PWN45_002175"
     CDS             complement(4141..5217)
                     /locus_tag="PWN45_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDL0623921.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            5401..6870
                     /locus_tag="PWN45_002176"
     CDS             5401..6870
                     /locus_tag="PWN45_002176"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL0623922.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            6863..7684
                     /gene="nadE"
                     /locus_tag="PWN45_002177"
     CDS             6863..7684
                     /gene="nadE"
                     /locus_tag="PWN45_002177"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL0623923.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            7955..8557
                     /locus_tag="PWN45_002178"
     CDS             7955..8557
                     /locus_tag="PWN45_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL0623924.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            8538..8711
                     /locus_tag="PWN45_002179"
     CDS             8538..8711
                     /locus_tag="PWN45_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDL0623925.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
BASE COUNT         3083 a         1250 c         1658 g         2880 t
ORIGIN      
        1 gtatatagat taatcaactt ttttggaaga gcaaatcacg caatcaacaa ataatataag
       61 aagtttttgc gatagtttta aaatagctgt aatagaatac taaatgtgac aaacttagaa
      121 ctaatatcaa gtgttgatgt tttgaatata aaaatgctaa tagatggcat cacgagattt
      181 atgactatct gttaaatcaa cctaacacga ttataatttg aataattggt taatatatga
      241 gtaattagaa aatagacaaa ggatgacgat ttatgtatat caatatgaaa gattatgggt
      301 taacaggcat aaacaaaact aaagatactc gagcaataca acgtgcgtta aatcgtggaa
      361 gatgtaaacc aacgacagtt tatataccga aagggacgta tgatatttgc aaaccattaa
      421 cgatatatgg caatacaaca cttttgttag ataatgaaac tattttacgc cgatgtcatt
      481 ctggtccttt attaaaaaat ggtcgtcgct ttggttttta tcgtggttat aatggacaca
      541 gtcatattca tattaaaggc ggcaagtttg atatgaatgg tgtatcgtat ccttataaca
      601 atacagctat gtgcattggg catgctgaag atattcaatt aataggtgtg accattaaga
      661 atgtagtgag tggtcatgca attgatgctt gtgggattaa cggactctat attaaaagct
      721 gttcatttga aggattcata gactatagtg gcgaacgctt ttattctgaa gcaatacaat
      781 tagacattca agtacctggt gcttttccaa aattcggaac gacagatggt acgataacga
      841 aaaatgtcat tatcgaagat tgttattttg gaccttcaga attgcccgaa atgggaagtt
      901 ggaatcgtgc tattggctca catgcaagta gacataatcg atactatgag aatattcata
      961 ttagaaataa tatatttgaa gatatacaag gttatgcatt aactcccttg aagtataaag
     1021 atgctttcat tattaataat aagtttatta actgtgaggg tggcattaga tatttaggag
     1081 ttagagatgg taaaaatgca gcagatgtga tgacaggaaa agacttaggt tcccaagcag
     1141 gcataaatat gaatataatt ggaaatgaat ttaaaggatc aatgtctaaa gatgcgatac
     1201 atgtacgtaa ttataataat gttaaacata aagatgtatt aatcgttggg aatacattca
     1261 ataattcgac tcaatcaatt catttagaag atattgatac agtgttttta agtcctgttg
     1321 aagcgggtat tcaagttact acaatcaatg tagatgaaat aaaaaagtaa aaagtttcgc
     1381 atgacattag gattaagaat agtagataat ttttgaaagc gcattcataa aacggtataa
     1441 atatgctata ataaacccaa ttatctgata aaaggggtat tttgacggta atgataatac
     1501 aagatagaca actttctata ctctaatata gtgagttgaa gtagcttgtc ataatcatca
     1561 tgagggggaa atttatggct tatttcaatc aacatcaatc aatgatatcg aaaaggtatt
     1621 taacattctt ttcaaaatca aagaaaaaga aaccgtttag tgcgggacaa cttattggac
     1681 taatattagg tccattactt ttcctattaa cattattatt ctttcatcca caagacttac
     1741 cttggaaagg cgtctatgtt ttagcgatta ctttatggat tgcgacttgg tggattactg
     1801 aagcaattcc tattgcagca acgagcttat taccaattgt gttattacca ttaggtcata
     1861 tacttacacc agaacaagta tcatccgaat atggcaatga tattatcttt ttgtttttag
     1921 gtggatttat tttggcaatt gcaatggaaa gatggaattt acatacgaga gtagcgttga
     1981 caattattaa tttgattggt gcaagcactt caaaaatatt acttggattc atggtggcaa
     2041 caggattctt atctatgttt gtatcgaaca ctgcagctgt aatgattatg attccgattg
     2101 gtttagcaat tattaaggaa gcacatgatt tacaagaagc caatacgaat caaacaagta
     2161 ttcaaaagtt tgaaaaatct ctagttttag caattggcta tgcaggtacg attggtggct
     2221 tgggtacatt aatcggaacc ccgccattaa ttattttaaa aggacaatac atgcaacatt
     2281 ttggatatga aattagtttt gctaaatgga tgattgtagg gattccaacg gtcattgttt
     2341 tgttaggtat tacttggctc tatttaagat atgttgcgtt tagacatgat ttgaaatatt
     2401 tacctggtgg tcagacgtta attaaacaaa agttagacga gcttggcaaa atgaagtatg
     2461 aagaaaaggt agtacaaact atctttgtac ttgctagctt attatggatt acaagagagt
     2521 ttcttctgaa aaaatgggaa gttacgtcat ctgttgcaga tggtacgatt gctattttta
     2581 tatcaatatt attatttatt attccagcta aaaatactga aaaacatcgc cgtatcattg
     2641 actgggaagt tgcaaaagag ctcccttggg gtgtattaat tttatttggt ggcggtttag
     2701 cattagcgaa aggtatttct gaaagtggtt tagcaaaatg gttaggcgaa cagttgaaat
     2761 cattaaatgg tgttagtccg attcttattg taattgtcat aacaatcttt gtcttatttt
     2821 taactgaagt gacatctaat actgcaactg caacgatgat tttaccgatt ttagcaacgt
     2881 tgtctgttgc tgttggagtg catccattac tacttatggc acctgcagct atggcggcta
     2941 actgtgcata catgttacca gtagggacac caccgaatgc aattatcttt ggttctggta
     3001 aaatatctat caaacaaatg gcatcagtag gattctgggt aaacttaatc agtgcaataa
     3061 ttattatttt agtcgtgtat tatgtaatgc ctatagtttt aggtattgat ataaatcaac
     3121 cactgccatt gaaatagtaa ttgcagatta gaacgaaaaa taaaaggtta cattagcaat
     3181 tgcttggacg agtggtaacg aaacgtatac cgcagcatcg tgtaaaaaca atacaaacaa
     3241 aagaaagtca accaaggatg gattcctatt ttaatccttg gttgactctt tattttattt
     3301 aaattgtaga acctagaaaa taaagtttaa ttaaaagcac caatcatttc tactttgaaa
     3361 tctaaggttt ctaaaatagc aatgactttc tttatatcgg ttgtaattgc agaatcagcc
     3421 tgaacgaaaa atcgatacat acctaattgt gtttttaaag gacgagactc aatccaggat
     3481 aaattaatat taaacaaagc aaatgtatta agcacacttg ctaacaaccc aggtttatca
     3541 tgcattggtg taattaaaaa catcaatgat gtcgcatttt gatcaaattg ctgctgattt
     3601 tttataacta aaaaacgtgt cacgttatgt ggatagtctt caatatgtgt atcaataggt
     3661 gtaaaaccat aagcttcgcc actacctaaa ggtgcaattg ctgcaacgcc attttcaatt
     3721 ttagtcaaac tttgaattgt actgtcgaca taatcatagt caaattgatg ttgatgtatg
     3781 taatttgttg tttgactgat tgctggtgca atagaataca cttttttaat atcagaaatg
     3841 gaatctgttc cattaccata taatgcaaag ttaatatcta aacgtatttc accgtgtgca
     3901 aagacatctt gctgtgcaag tgcatctgcc acaatgttga ttgttccttc tatagaattt
     3961 tcaataggga caacaccaat cgatgtgtca tcatctgcaa ctgccttgat gacttcaaaa
     4021 aaatttgact ttggttgaaa agttgcttca ttttcagaaa aatactgacg acaagccaaa
     4081 tatgaaaatg tacctttagg gcctaaataa tataattgca tatgctacac ctctactaac
     4141 ttaatgatgg aaagggcact ggttagcatt tgattctttc tttttataga aaaagtttgg
     4201 atcttttact gtattgtcat atccgtgatg ataatttgac gtcaatgttg gagataatgg
     4261 cggtgctagc caagaccatt ttccggtaac ttgacgacct tgttgtgctt cgttacgttc
     4321 gaatagttcg aattgctttg cagcggtcaa atgatcgaca attgatacgc cttctttttt
     4381 aaaggaatga tacacagcat agttcaattc aacaagtgct cgatctttat taaatgaatt
     4441 atttttaagt gtatcaaatt caaacgcatc tgcaactttt tctagtaaat tgtaacggta
     4501 atcatcaata aagttacgta cgccaatttc agttaccata taccaaccgt taaagggtgc
     4561 agttggatat acaatgccac cgatttttaa gtccatattg gaaatgatag ggactgcata
     4621 ccattttaag ttcaattttc ttaattttgg ataatgatta tgttcaatag gtacttcttt
     4681 aattaatgaa gtaggatatt cgtaaaattt aactgactca ttaggtaatt ggtaaatcag
     4741 tggtaacacg tcaaaattag taccttttcc tttccaacct aagtgatttg ctaagcgtgt
     4801 aacttctttt tcagcaggat caccacaatt gtcatagcca gcatagcgaa ttaattgatt
     4861 gttgaaaatt ttaggtccat cctttggagc atatatagta atatacggct ttaatttacc
     4921 ttcatttgta gcctgtgtaa tatgataagt aattgatgat aagaacgatg cttcgtcagt
     4981 aacatctctt gcatcaatga catttaacga atcccaaaat aaacgaccaa tgcaacgatt
     5041 tgaattacgc caagccattt tagcaccata aataagttct tcttctgtat gtgtatatgt
     5101 cccagtttct tttatttcta gttcaatgtc atgtaaacgt ttattgataa tttgcgtttc
     5161 ataatgacac tctttataca tgttttctat gaaagcttga gcctctttaa ataacattaa
     5221 caacacctcg ctttatatta tagtctacat tattaaaata ctcttaaaaa ttatgtatat
     5281 gtcattaaat tgttggttga ttttaattaa aagtatggaa attaaggggc tcttatgtat
     5341 ataaaaaaat gaattatgat aaaatgtaag aaaatattta ggtcgattgg agagatacaa
     5401 gtgtaccaat tagaagacga cagtttaatg ttacataatg acttatatca aataaatatg
     5461 gctgaaagtt attggaatga taatattcat gaaaaaatgg ctgtatttga tttgtatttt
     5521 agaaaaatgc catttaatag tggctatgct gtttttaatg gtttaaaacg cgtcattgat
     5581 tttatagaac attttggatt ttcagaatct gatttggaat atttaaagtc tattggctac
     5641 aaggatgatt tcttatcata tttaaaagat ttaaaattca caggcagcat ccgttcgatg
     5701 caagaaggcg aattatgctt tggtaacgaa ccattgttac gcgtagaagc accattgatt
     5761 caagcgcaat taatagaaac aattttatta aacattgtaa atttccatac attaattaca
     5821 acaaaggcta gcagaattcg tcaaattgca tcaaatgata aattaatgga gtttggtaca
     5881 cgtcgtgcgc aagaaattga tgcagcattg tggggcgcta gagctgctta catcgggggc
     5941 tttgattcta caagtaatgt tagggcgggg aaattatttg gtatacctgt gtctggtaca
     6001 catgcacatg catttgtcca aacttatgga gacgaatatg ttgccttcaa aaaatatgct
     6061 gaaagacata aaaattgtgt gttcctagta gatacattcc atactttaaa atctggcgtg
     6121 ccaaatgcaa taaaagttgc aaaagaatta ggtgacaaaa ttaactttgt aggtattcga
     6181 ttagattctg gagatatcgc ttatttatct aaagaggcaa gacgtatgct tgatgaagca
     6241 ggatttactg aaactaaaat tatcgcgtct aatgatttgg atgaagaaac gattacgagt
     6301 ttgaaagcac aaggtgcaaa agtagattct tggggcgttg gtacaaagct gattacagga
     6361 tacgatcaac cagcattagg tgcagtatat aaacttgtag ctattgaaaa tgaagatggt
     6421 tcatatagtg atcgtattaa attatcaaat aacgctgaaa aggttacgac gccaggtaag
     6481 aaaaatgtat atcgcattat aaacaagaaa acaggtaagg cagaaggcga ttatattact
     6541 ttggaaaatg aaaatccata cgatgaacaa cctttaaaat tattccatcc agtgcatact
     6601 tataaaatga aatttataaa atctttcgaa gccattgatt tgcatcataa tatttatgaa
     6661 aatggtaaat tagtatatca aatgccaaca gaagatgaat cacgtgaata tttagcacta
     6721 ggattacaat ctatttggga tgaaaataag cgtttcctga atccacaaga atatccagtc
     6781 gatttaagca aggcatgttg ggataataaa cataaacgta tttttgaagt tgcggaacac
     6841 gttaaggaga tggaagaaga taatgagtaa attacaagac gttattgtac aagaaatgaa
     6901 agtgaaaaag cgtatcgata gtgctgaaga aattatggaa ttaaagcaat ttataaaaaa
     6961 ttatgtacaa tcacattcat ttataaaatc tttagtgtta ggtatttcag gaggacagga
     7021 ttctacatta gttggaaaac tagtacaaat gtctgttaac gaattacgtg aagaaggcat
     7081 tgattgtacg tttattgcag ttaaattacc ttatggagtt caaaaagatg ctgatgaagt
     7141 tgagcaagct ttgcgattca ttgaaccaga tgaaatagta acagtcaata ttaagcctgc
     7201 agttgatcaa agtgtgcaat cattaaaaga agccggtatt gttcttacag atttccaaaa
     7261 aggaaatgaa aaagcgcgtg aacgtatgaa agtacaattt tcaattgctt caaaccgaca
     7321 aggtattgta gtaggaacag atcattcagc tgaaaatata actgggtttt atacgaagta
     7381 cggtgatggt gctgcagata tcgcacctat atttggtttg aataaacgac aaggtcgtca
     7441 attattagcg tatcttggtg cgccaaagga attatatgaa aaaacgccaa ctgctgattt
     7501 agaagatgat aaaccacagc ttccagatga agatgcatta ggtgtaactt atgaggcgat
     7561 tgataattat ttagaaggta agccagttac gccagaagaa caaaaagtaa ttgaaaatca
     7621 ttatatacga aatgcacaca aacgtgaact tgcatataca agatacacgt ggccaaaatc
     7681 ctaatttaat tttttcttct aacgtgtgac ttaaattaaa tatgagttag aattaataac
     7741 attaaaccac attcagctag actacttcag tgtataaatt gaaagtgtat gaactaaagt
     7801 aagtatgttc atttgagaat aaatttttat ttatgacaaa ttcgctattt atttatgaga
     7861 gttttcgtac tatattatat taatatgcat tcattaaggt taggttgaag cagtttggta
     7921 tttaaagtgt aattgaaaga gagtggggcg ccttatgtca ttcgtaacag aaaatccatg
     7981 gttaatggta ctaactatat ttatcattaa catttgttat gtaacgtttt taacgatgcg
     8041 aacaatttta acgttgaaag gttatcgtta tattgctgca tcagttagtt ttttagaagt
     8101 attagtttat atcgttggtt taggtttggt tatgtctaat ttagaccata ttcaaaatat
     8161 tattgcctac gcatttggtt tttcaatagg tatcattgtt ggtatgaaaa tagaagaaaa
     8221 actggcatta ggttatacag ttgtaaatgt aacttcagca gaatatgagt tagatttacc
     8281 gaatgaactt cgaaatttag gatatggcgt tacgcactat gctgcgtttg gtagagatgg
     8341 tagtcgtatg gtgatgcaaa ttttaacacc aagaaaatat gaacgtaaat tgatggatac
     8401 gataaaaaat ttagatccga aagcatttat cattgcgtat gaacctcgaa acatacatgg
     8461 tggattctgg actaaaggca ttcgtcgtag aaagcttaaa gattatgaac cagaagaact
     8521 ggaaagtgta gtagaacatg aaattcaaag taaatgagaa tgaaacaatt gctgattgtt
     8581 tgtcacgaat gaaaatgcaa gggtatatgc cggtaaaacg tattgaaaaa cccgtgtttc
     8641 aagagcaaaa agatggcacg gttgaagtat cacatcaaga aatcgttttt gtaggtaaga
     8701 aaatccaata acataatcca atttaaataa agactatttg aagaggaaag gctattcaaa
     8761 gtttgagtaa ttttactttg aatagcctat ttgtttatac atgcaagatg ctcgatccat
     8821 attgtatgag aaacccccag caagctatat aaagcatatg ctgggggttc t
//
LOCUS       DANJFZ010000105         8815 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.105, whole genome shotgun sequence.
ACCESSION   DANJFZ010000105 DANJFZ010000000
VERSION     DANJFZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.105"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            10..678
                     /gene="capA"
                     /locus_tag="PWN45_002180"
     CDS             10..678
                     /gene="capA"
                     /locus_tag="PWN45_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDL0623926.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            694..1380
                     /gene="cap8B"
                     /locus_tag="PWN45_002181"
     CDS             694..1380
                     /gene="cap8B"
                     /locus_tag="PWN45_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDL0623927.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1383..2147
                     /gene="cap8C"
                     /locus_tag="PWN45_002182"
     CDS             1383..2147
                     /gene="cap8C"
                     /locus_tag="PWN45_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDL0623928.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2167..3991
                     /gene="cap8D"
                     /locus_tag="PWN45_002183"
                     /pseudo
     CDS             2167..3991
                     /gene="cap8D"
                     /locus_tag="PWN45_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3981..5009
                     /gene="cap8E"
                     /locus_tag="PWN45_002184"
     CDS             3981..5009
                     /gene="cap8E"
                     /locus_tag="PWN45_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDL0623929.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5022..6131
                     /gene="cap8F"
                     /locus_tag="PWN45_002185"
     CDS             5022..6131
                     /gene="cap8F"
                     /locus_tag="PWN45_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDL0623930.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6135..7259
                     /gene="cap8G"
                     /locus_tag="PWN45_002186"
     CDS             6135..7259
                     /gene="cap8G"
                     /locus_tag="PWN45_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDL0623931.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7262..7888
                     /locus_tag="PWN45_002187"
     CDS             7262..7888
                     /locus_tag="PWN45_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDL0623932.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7893..>8815
                     /locus_tag="PWN45_002188"
     CDS             7893..>8815
                     /locus_tag="PWN45_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0623933.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEELIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYL"
BASE COUNT         3250 a         1210 c         1717 g         2638 t
ORIGIN      
        1 aggtaaataa tggaaagtac attagaatta acaaaaatta aagaagtatt acaaaaaaac
       61 ttgaagattt taattatttt accgctatta tttttaatta ttagcgctat tgttacattt
      121 ttcgtcttat cacctaaata tcaagctaat actcaaattt tagtgaatca aactaagggt
      181 gacaatcctc agtttatggc gcaagaggtt caaagtaata ttcaacttgt aaatacgtat
      241 aaagaaattg ttaaaagtcc tagaatttta gatgaggtgt caaaggactt aaatgataag
      301 tattcaccat ctaaattgtc gagtatgttg acaattacaa accaagaaaa tacgcaactt
      361 atcaacatcc aagttaaaag tggtcataaa caagattcgg aaaaaattgc gaatagcttc
      421 gctaaagtta caagtaaaca aattccgaag attatgagtg tggataacgt atcaatttta
      481 tctaaagcag acggtacagc agttaaagtc gcaccaaaaa ctgtagtgaa tctaatcggt
      541 gcattctttt taggattagt tgtcgcgctt atatatatct tcttcaaagt aattttcgat
      601 aagcgaatta aagatgaaga agatgtagag aaagaattag gattgcctgt attgggttca
      661 attcaaaaat ttaattaagg atggttgcta cttatgtcaa aaaaggaaaa tacgacaaca
      721 acactatttg tatatgaaaa accaaaatca acaattagtg aaaagtttcg aggtatacgt
      781 tcaaacatca tgttttcaaa agcaaatggt gaagtaaagc gcttattggt tacttctgaa
      841 aagcctggtg caggtaaaag tacagttgta tcgaatgtag cgattactta tgcacaagca
      901 ggctataaga cattagttat tgatggcgat atgcgtaagc caacacaaaa ctatattttt
      961 aatgagcaaa ataataatgg actatcaagc ttaatcattg gtcgaacgac tatgtcagaa
     1021 gcaattacgt cgacagaaat tgaaaattta gatttgctaa cagctggccc tgtacctcca
     1081 aatccatctg agttaattgg gtctgaaagg ttcaaagaat tagttgatct gtttaataaa
     1141 cgttacgaca ttattattgt cgatacaccg ccagttaata ctgtgactga tgcacaacta
     1201 tatgcgcgtg ctattaaaga tagtctgtta gtaattgata gtgaaaaaaa tgataaaaat
     1261 gaagttaaaa aagcaaaagc acttatggaa aaagcaggca gtaacattct aggtgtcatt
     1321 ttgaacaaga caaaggtcga taaatcttct agttattatc actattatgg agatgaataa
     1381 gtatgattga tattcataac catatattgc ctaatatcga tgacggtccg acaaatgaaa
     1441 cagagatgat ggatctttta aaacaagcga caacacaagg tgttacagaa atcattgtaa
     1501 catcacatca cttacatcct cgatatacca cacctataga aaaagtgaaa tcatgtttaa
     1561 accatattga aagcttagag gaagtacaag cactaaatct aaagttttat tatggtcagg
     1621 aaataagaat taccgatcaa atccttaatg atattgatcg aaaagttatt aacggtatta
     1681 atgattcacg ctatttacta atagaatttc catcaaatga agttccacac tatactgatc
     1741 aattattttt cgaattacag agtaaaggct ttgtaccgat tattgcacat ccagagcgga
     1801 ataaagcaat aagtcaaaac cttgacatac tatacgattt aattaacaaa ggtgctttaa
     1861 gtcaagtgac aacggcgtca ttagcgggta tttccggtaa aaaaattaga aaattagcaa
     1921 ttcaaatgat tgaaaacaat ctgacacatt tcatcggttc agatgcgcat aacacagaaa
     1981 tcagaccgtt cttaatgaaa gacttattta atgataagaa attacgtgat tattatgaag
     2041 atatgaacgg atttattagt aatgcgaagt tagttgttga tgataaaaaa attcctaaac
     2101 gaatgccaca acaagattat aaacagaaaa gatggtttgg gttataaaca gcaaatgagg
     2161 ggttttatgg cacatttatc tgtgaaattg cggcttttaa tactagcatt aatcgattca
     2221 ctgatagtga cattttcagt attcgtaagt tattacattt tagaaccgta tttcaaaaca
     2281 tattctgtca aattattaat attggcagct atatcactat tcatatcgca tcatatttca
     2341 gcatttattt ttaatatgta tcatcgagcg tgggaatatg ccagtgtgag tgaattgatt
     2401 ttaattgtta aagctgtgac gacatctatc gttattacga tggtggtcgt gacaattgtt
     2461 acaggcaata gaccgttttt tagattgtat ttaattactt ggatgatgca cttgatttta
     2521 ataggtggct caaggttatt ttggcgtatt tatcggaaat accttggagg taagtcattt
     2581 aataagaagc caactttagt tgttggtgct ggtcaagcag gttcaatgct gattagacaa
     2641 atgttgaaaa gtgacgaaat gaaacttgaa ccggtattag cagtcgatga tgacgaacat
     2701 aaacgcaata tcacaattac tgagggtgta aaagtccaag gtaaaattgc ggatattcca
     2761 gaactagtga ggaaatataa gattaaaaaa atcatcattg caattccaac tattggtcaa
     2821 gagcgtttga aagaaattaa taatatttgc catatggatg gcgttgagtt attgaaaatg
     2881 ccaaatatag aagacgtcat gtctggtgag ttagaagtga accaacttaa aaaagttgaa
     2941 gtagaagatt tactaggcag agatcctgtt gaattagata tggatatgat atcaaatgaa
     3001 ttgacgaata aaactatttt agttacgggt gcaggtggtt caataggatc agaaatttgt
     3061 agacaagttt gtaatttcta tccagaacgt attattctac ttggccatgg tgaaaacagt
     3121 atttatttaa tcaatcgtga attgcgaaat cgcttcggaa aaaaatgttg atatcgttcc
     3181 tattatagcg gatgtgcaaa atagagcgcg tatgtttgaa attatggaaa cgtataaacc
     3241 atacgcagtt tatcatgcag cagcacacaa gcacgtgccg ttaatggaag acaaccctga
     3301 agaagcagta cgtaataata ttttaggtac gaaaaatact gctgaagctg ctaaaaatgc
     3361 agaggtaaag aaattcgtta tgatttctac ggataaagcc gttaatccgc ctaatgtcat
     3421 gggagcttca aagcgaattg cagaaatgat tattcaaagt ttaaatgatg aaacgcatcg
     3481 aacaaatttt gttgcagtga gatttggtaa tgtacttgga tcgagaggat ctgtgattcc
     3541 acttttcaaa agtcaaattg aagaaggtgg gccagttact gtgacacatc ctgaaatgac
     3601 acgttacttt atgacaattc ctgaagcttc tagactagtt ttgcaggcag gggcattagc
     3661 agaaggtggc gaagtatttg tgctagatat gggagaacca gtgaaaattg tagatttggc
     3721 acgtaattta attaagctaa gtggtaaaaa agaagacgac atacgcatta cttatacagg
     3781 gattagaccc ggcgaaaaaa tgtttgaaga gcttatgaat aaagatgagg ttcatcctga
     3841 acaagtattt gaaaaaattt atcgtggcaa agtacaacat atgaaatgta atgaagttga
     3901 agcgattatt caagacatcg tcaatgactt tagtaaagaa aaaattatta actatgccaa
     3961 tggcaaaaag ggagataatt atgttcgatg acaaaatttt attaattact gggggcacag
     4021 gatcattcgg taatgctgtt atgaaacagt ttttagattc taatattaaa gaaattcgta
     4081 ttttttcacg cgatgagaaa aaacaagatg acattcgaaa aaaatataat aattcaaaat
     4141 taaagttcta cattggtgat gtgcgtgata gtcaaagtgt agaaacagca atgcgagatg
     4201 tttattacgt attccatgca gcagctttaa aacaagtgcc gtcatgtgaa ttctttccag
     4261 ttgaggcagt gaagacaaat attattggta cagaaaatgt cttacaaagt gctattcatc
     4321 aaaatgttaa aaaagtcata tgtttatcta cagataaggc agcgtatcct attaatgcta
     4381 tgggtatttc aaaagcaatg atggaaaaag tattcgtagc caaatcaaga aatattcgta
     4441 gtgaacaaac gcttatttgt ggtacaagat acggtaatgt gatggcttca agaggatcag
     4501 taataccttt gtttatcgac aaaatcaaag ctggagaacc tttaacgatt acagatcctg
     4561 atatgacaag atttttaatg agcttagaag atgcggtaga actagttgtt catgcattta
     4621 agcatgcaga gacaggagat attatggttc aaaaagcacc aagctcaacg gtaggggatc
     4681 ttgcgaccgc attattagaa ttgtttgaag ctgataatgc aattgaaatc attggtacgc
     4741 gacatggaga gaaaaaagca gaaacattgt tgacgagaga agaatacgca caatgtgaag
     4801 atatgggtga ttattttaga gtgccggcag actccagaga tttaaattat agtaattatg
     4861 ttgaaaccgg taacgaaaag attacgcaat cttatgaata taactccgat aatacacata
     4921 ttttaacggt ggaagagata aaagaaaaac ttttaacact agaatatgtt agaaacgaat
     4981 tgaatgatta taaagcttca atgagatagg agagattgac gttgaatatt gtaattacag
     5041 gagcaaaagg ttttgtagga aaaaacttga aagcagattt aacttcaacg acagatcatc
     5101 atattttcga agtacatcga caaactaaag aggaagaatt agagtcagca ttgttgaaag
     5161 cagactttgt cgtgcattta gcgggtgtta atcgacctga acatgacaaa gaattcagct
     5221 taggaaacgt gagttattta gatcatgtac ttgatatatt aactagaaat acgaaaaagc
     5281 cagcgatatt attatcgtct tcaatacaag caacacaaga taatccttat ggtgagagta
     5341 agttgcaagg ggaacagcta ttaagagagt atgccgaaga gtatggcaat acggtttata
     5401 tttatcgctg gccaaattta ttcggcaagt ggtgtaagcc gaattataac tcagtgatag
     5461 caacattttg ttacaaaatt gcacgtaacg aagagattca agttaatgat cggaatgttg
     5521 aactaacgct aaactacgtg gatgatatcg tcgctgaaat aaagcgtgct attgaaggaa
     5581 ctccaacgat tgaaaatggt gtacctacag taccaaacgt atttaaagtg acattgggag
     5641 aaattgtaga tttattatac aagttcaaac agtcacgtct cgatcgaaca ttgccgaaat
     5701 tagataactt gtttgaaaaa gatttgtata gtacgtattt aagctatcta cctagtacag
     5761 actttagtta tcccttactt atgaatgtgg atgatagggg ttcttttaca gaatttataa
     5821 aaacaccgga tcgtggtcaa gtttctgtaa atatttctaa accaggtatt actaaaggta
     5881 atcactggca tcatactaaa aacgaaaaat ttctagtcgt atcaggtaaa ggggtaattc
     5941 gttttagaca tgttaatgat gatgaaatca ttgaatatta tgtttctggc gacaaattag
     6001 aagttgtaga cataccagta ggatacacac ataatattga aaatttaggc gacacagata
     6061 tggtaactat tatgtgggtg aatgaaatgt ttgatccaaa tcagccagat acgtatttct
     6121 tggaggtata gcgcatggaa aaactgaaat taatgacaat agttggtaca aggcctgaaa
     6181 tcattcgttt atcatcaacg attaaagcat gtgatcaata ttttaatcag atattagtac
     6241 acactggtca aaattatgat tatacattga atcaaatttt ctttgatgat ttggaattaa
     6301 gacaaccgga ccactactta gaggcagttg gaagtaacct tggagaaacg atggggaata
     6361 ttattgcgaa gacatatgat gttttattac gcgaacaacc agatgcactt ttaattcttg
     6421 gtgatacaaa tagttgttta gcagcagtat ctgctaaacg attaaagatt cctgtgttcc
     6481 acatggaagc gggtaataga tgctttgatc agaatgtacc tgaagaaatc aatcgtaaaa
     6541 ttgttgacca tgtcagtgat gtgaatctac cttatacgga acatagcaga cgttatttat
     6601 tagatgaagg cctcaataaa gcgaatatct ttgtgacagg atcaccgatg acagaagtga
     6661 tagaagcgca tcgagataaa attaatcaca gtgacgtttt aaataaacta ggattagaac
     6721 cgcaacaata cattttagta tctgcgcata gagaagagaa tatcgataat gaaaagaatt
     6781 ttaaatcatt aatgaatgcg ataaatgata ttgccaaaaa gtataaaatg cctgtgattt
     6841 attcaacgca tccaagaagt tggaagaaaa ttgaagaaag taaatttgaa tttgatccat
     6901 tagttaaaca gttaaagcca tttggtttct ttgattataa tgcattgcaa aaagatgcat
     6961 ttgttgtgct atcagatagt ggaacattgt cagaagagtc gtctattttg aagttccctg
     7021 gtgtccttat tcgaacttcc acagaaagac cggaagtact agataaaggt acggttattg
     7081 taggtggtat tacctataac aatctaatcc aatccgttga actagcaaga gagatgcaaa
     7141 acaataacga accgatgatt gatgctattg attataaaga cactaacgtt tcgacaaagg
     7201 tagttaaaat tattcaaagc tataaagata ttatcaatcg aaatacttgg aggaaatgac
     7261 gatgaggata gcgattgaaa agataattgg tttgctgaaa aaccagtcct ctaaagaatc
     7321 gaatgttaag attcatcgct tggcgtatat tacaaactca aaatttgatg gcaataacta
     7381 tatagataga tggtgtaaaa tcaggaattc tcacattggt gaatacagtt atattggatt
     7441 tggtagtgat tttaataatg tagaagtagg aagatattgt tcgatatctt cggatgtaaa
     7501 aattgggtta ggaaaacatc ctacacactt ttttagctca tcaccgattt tttattctaa
     7561 taataatcca tttaacataa agcaaaagtt tatagacttt aatgaccaac caagccgtac
     7621 aacaattaaa aatgatgtgt ggattggtgc aaatgtaatt attatggatg gtttaacaat
     7681 aaatactggt gcagtcatag cagccggctc agttgttact aaaaatgtag gagcatatga
     7741 ggttgttggt ggtgttcctg caaaagtgat taagaagcga tttgacaata aaacaattga
     7801 aaaacttttg gaaagcaagt ggtgggagaa aacgcctgac aaactaaaag gattttcggt
     7861 tgaatattta aataaaaagg atacttaatg atatgagaat tttaaatatt gtatcgagta
     7921 atattgttca agacccaagg gtacttaaac aaatagaaac aattaaaggc gttacggatg
     7981 attataaaat tgttggaatg aataattcac aagctactaa taagcgattg gaaaatttag
     8041 attgtaatta tcgtttgtta ggtagcaagg tagatccaaa aaatattctt tctaaattaa
     8101 ttaagcgtat aagatttgca acaggtgtta tccgagaaat taaagcttat aaacctgacg
     8161 tgattcatgc aaatgatttc gacgtattat taatggtcta tttaagcaat tataaaaaag
     8221 ctaatattgt ttatgatgcg catgaaatat atgcgaaaaa tgcctttatt aataaagttc
     8281 cacttatttc aaagtttgta gaaagtatag aaaaacacat agtaaaacat cgtgttaatg
     8341 ccttcgtaac agtaagtcat gcagcaaaag aatattatca atctaaagga tataagaagg
     8401 aagcgaatgt tattacgaat gcacctattt taaatgatag cagagaattt aaagaaatcg
     8461 aaaactttaa agaaattgta tatcaaggtc aaattgtaat ggacagagga tatgaagagt
     8521 ttattattgc ttcatcagct tttaaacaaa atgctccttc attcataatt cgagggtttg
     8581 gtccgcatga agaattgata aaagaactga ttagttataa cccggaaaat attaggttgg
     8641 ataaaccagt tgaagtaaaa gaattggttg ataagttagc agaaagtaat gttggtgttg
     8701 tcttgacgaa accagtatct attaattttg aatatacagt atctaataaa atttttgaat
     8761 gtatacatgc tggtttacca gtaattttat ctcctgtcaa agagcatatt tatct
//
LOCUS       DANJFZ010000106         8788 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.106, whole genome shotgun sequence.
ACCESSION   DANJFZ010000106 DANJFZ010000000
VERSION     DANJFZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.106"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            78..1202
                     /locus_tag="PWN45_002189"
     CDS             78..1202
                     /locus_tag="PWN45_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0623934.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            1507..2325
                     /locus_tag="PWN45_002190"
     CDS             1507..2325
                     /locus_tag="PWN45_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDL0623935.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            2343..2957
                     /locus_tag="PWN45_002191"
     CDS             2343..2957
                     /locus_tag="PWN45_002191"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL0623936.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            2950..4200
                     /locus_tag="PWN45_002192"
     CDS             2950..4200
                     /locus_tag="PWN45_002192"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL0623937.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            4216..5229
                     /locus_tag="PWN45_002193"
     CDS             4216..5229
                     /locus_tag="PWN45_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDL0623938.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            5198..5776
                     /gene="hisB"
                     /locus_tag="PWN45_002194"
     CDS             5198..5776
                     /gene="hisB"
                     /locus_tag="PWN45_002194"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDL0623939.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            5773..6351
                     /gene="hisH"
                     /locus_tag="PWN45_002195"
     CDS             5773..6351
                     /gene="hisH"
                     /locus_tag="PWN45_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL0623940.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            6344..7048
                     /locus_tag="PWN45_002196"
     CDS             6344..7048
                     /locus_tag="PWN45_002196"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL0623941.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            7045..7803
                     /gene="hisF"
                     /locus_tag="PWN45_002197"
     CDS             7045..7803
                     /gene="hisF"
                     /locus_tag="PWN45_002197"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL0623942.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            7800..8432
                     /locus_tag="PWN45_002198"
     CDS             7800..8432
                     /locus_tag="PWN45_002198"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL0623943.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
BASE COUNT         3128 a         1304 c         1761 g         2595 t
ORIGIN      
        1 acagtttctt aaaaagttat gagccacata ataaaaatgt tataattgtg cataataaaa
       61 catagggaga atgtttaatg cataaaaaat atttattgct attttttctg ttattatcag
      121 ttgcattgtt atatagctgt tccaagcaaa atgagaaagc tcaaaataaa tctgttaatc
      181 caaatgagta tgcaaagcat aaatcgaaaa tggatgaaca ttgggaaaca tatcacggcc
      241 aagtttatca tgtgttttat cacccattaa ttacagaccc aaaagttgcg tttacaggag
      301 atacacacca agctaagggc aacaatgatt ggatgattac tgtaagtgaa tttaaaaaat
      361 cattgaacga attatataaa aataattaca tcatcgttaa tccacatgat gtatacgact
      421 tatcatctca tcatgtaaaa gccaaaaaaa taaaattgcc gaaaggtaaa aagccactaa
      481 tcatctcaat tgatgatatg aattactata gttacatgag acaccatggt tatgctgacc
      541 gtttagtact cgataaaaat aaacatgttg tttctgaaac tacagacaaa aaaggccatg
      601 ctacttattc tgatgacaat gatatcgtgc caattttaaa taaatttgta aaagagcatc
      661 cagatttttc tttaaatggt gaaaaaggtg ttgttgcttt aaccggttat gagggcgtat
      721 taggttatcg cacgaatgaa ttaacgagta aagattatac aaagaataaa aaggcagctg
      781 aggaagttgt tcgcgcaatg aaacgggatg gatggtcatt tgcgagtcat tcatatggtc
      841 acattaattt tgaaaaaaca tctttagaag gtattaaacg tgatactaaa agatggaaag
      901 atgaagttga accaattgta ggtaaaacag atatgtttgt atttccacat ggcgcacagg
      961 atagacatac acaagcgtat gattatttgg tagatgaagc agagtttaag tttattgcag
     1021 gtgtcggtcc aaacaatttt acagatatat cagcaacgaa cgtttaccaa gatagggttg
     1081 cgattgatgg tctaaactta tttgaattta aatataaact caaaccgttt tttaatcctg
     1141 aaaatgtata tagtaaacaa gatagacgtt actttaaagg gaatcgggat tatgaagaat
     1201 agttaaattt aataaaaaga tagaaagaaa ataactttac ataataaggc aaggacaata
     1261 tgtgcaagaa gtacaacttc aatataaaaa tcttggttaa gcatttttat aggcacaaat
     1321 gtcttgcctt ttttatttta aaaaatttta aaatcaatac accgaatttt ctgaaaaagg
     1381 ttgtaatgta ttgctaaaaa tgataagtta ttaatcatat tacactttag cgtggtagag
     1441 aagtaaagca aacaacgaaa ataccaagtg attttaaata cagtattgaa agtaaagggt
     1501 ggtcgagtga ataattcaga acaattaatt gcgttaaaag aatcggaaac agcattttta
     1561 aaatatttta ataaagcgga ttatgagtta gttgatttta gtgtggttga aaagttggac
     1621 tggaaacaat tgaaccatga ggatttgcag cagatgggtg agcgaaattt ttggcaacat
     1681 gagcatcaaa tttatgcact tcgaaatgat tttacagatc aattattgcg ttattactca
     1741 atgtatccaa ctgcagcaac aaaagtggca tacacaggtt taatcattcg gaataatgaa
     1801 gcggctgtac aagttggttt ggaaaactat gcaccatcat tagcgaatgt gcaacagagc
     1861 ttaaagctct ttattcagtt tattcaacag cagttgagag acaacgtgca ttttgttgta
     1921 ttgggacatt atcaattgct tgatgcctta ctcgataaat cgttacaaac accagacata
     1981 ctatcaatga ttgaggaacg taatttatcg ggactggtta catatttgtc gactgaacat
     2041 ccaatcgtgc aaatcttgaa agagaatacg caacaacaat taaatgtatt ggaacactat
     2101 attccaaatg accatccagc actcgttgaa ttaaaaatat gggaacgttg gttacataaa
     2161 caaggttaca aagacatcca tttagatatt actgcgcagc cacctagatc ctattacaca
     2221 ggtttattta ttcaatgtca ttttgctgaa aatgaatctc gagttttaac aggtggttat
     2281 tacaaaggaa gcatcgaagg gtttggatta ggattaacac tttaagtaag ggagtatgca
     2341 caatgttaag aatcgccata gccaaaggac gtctaatgga tagtttaatt aactatttag
     2401 atgtaattga atatacgaca ttatcagaaa cattaaaaaa tagagaacgc caattattat
     2461 taagtgtaga taatattgaa tgcattttag taaaaggaag tgacgtgcca atctatgtgg
     2521 aacaaggaat ggcagacata ggcattgttg gtagcgacat attagatgag cgccaatata
     2581 atgttaataa tttgttgaat atgccttttg gagcatgtca ttttgcggtt gcagcgaaac
     2641 ctgaaacgac caattatcgt aaaatcgcaa cgagttatgt tcatactgct gaaacatatt
     2701 ttaaatcaaa aggtattgat gtcgaattga ttaaattgaa tggctctgtt gaattggccg
     2761 gtgttgtaga tatggtagac ggaattgtcg acatcgttca aacaggtact acgctaaaag
     2821 cgaacggact ggttgaaaag caacatatta gtgatatcaa tgcaagatta ataactaata
     2881 aagcagctta ttttaaaaaa tcacaattaa tagagcaatt tattcgctct ttggaggtgt
     2941 ctattgccaa tgcttaatgc acaacaattt ttaaatcaat tttcattaga agcaccatta
     3001 gatgagtcat tgtatccaat tattcgcgat atttgtcagg aagttaaagt tcatggagat
     3061 aaggctttga aaatgtataa tctaacattc gatcatacga aaacagatca tttagaaatt
     3121 agtcatgaac aaattaaagc agcatttgac acattagatg aaaaaacaaa acaagcatta
     3181 caacaaagtt atgaaagaat taaagcatat caagaaagta ttaaacagac gaatcaacag
     3241 ttagaagaat cagtggagtg ttatgaaata taccatccac tagaaagtgt cggtatttat
     3301 gtgcctggtg gtaaagcaag ttatccttca acggtattaa tgacagcgac gttagcacaa
     3361 gtagcaggtg tggaaaatat tgtcgttgtg acaccacctc aacctaatgg tgtgtctcaa
     3421 gaagtattag cagcatgcta tattacgcaa gttgatcaag tatttcaagt tggtggtgct
     3481 caaagtattg ctgcgttaac ttatggcaca gaaacgatac ctaaagttga taagattgta
     3541 ggtccaggca atcaatttgt tgcatatgcc aagaaatatt tatttggaca agtaggtatt
     3601 gaccaaatag ctggaccaac agaaatagca cttattattg atgaaacagc agatttagat
     3661 gccatcgtat atgatgtttt tgcacaagca gaacatgatg aattagcacg tacatatgcc
     3721 attagtgaag atgcgcaagt ccttaaagat ttagaatcac gtattgctaa agcattgcct
     3781 aatgtggaca gatacgacat tgtttctaaa agtatcgcta atcaacacta ccttatccat
     3841 gctagtaatt ttgatgaagc atgccatgtc atgaatacaa tcgcgcctga acatgcgtcg
     3901 attcaaacag taaatcctca accatatatt gaaaaagtga aatatgtggg tgcattgttt
     3961 attggacatt attcgccaga ggtcatagga gattacgttg caggtccaag tcatgtatta
     4021 cctacaaata gaacagctag atttaccaat gggttatcgg tcaatgattt cttaacacgg
     4081 aacacggtca tccatttatc aaaagatacg tttgaacaaa ttgctgattc agcacaacat
     4141 attgctcatg ttgaagcatt atacaatcac cagcagtcta ttttaatacg tcagtcttag
     4201 gggagtgtaa ttgaaatgat ttatattgat aaaaatgaaa gtccagttac gccgttggat
     4261 gaaaaaacaa tgacgtctat tattagtgca acgccatata atttatatcc tgatgcagca
     4321 tatgaacaat tcaaggaagc ttatgctaag ttttacggat tatcgcctga acaaattatt
     4381 gcaggaaatg gatctgatga attgattcaa aagttaatgc tgatcatgcc agaaggtccg
     4441 gcattaacgc taaatcctga tttttttatg tatcaagcat atgcggcaca agtaaatcgt
     4501 gaaattgcat ttgtagatgc aggatcagat ttaacgtttg atttggaaac cattttaacg
     4561 aaaatcgatg aagtacaacc atcatttttt attatgagta atccacataa cccttcaggc
     4621 aagcaatttg atacggcatt tttaacagct attgcagata agatgaaagc attaaacgga
     4681 tactttgtca ttgatgaagc atatttagat tatggtacgg catatgacgt ggaactggca
     4741 ccacacatct taagaatgcg tacattatca aaggcgtttg gaattgccgg cttaagatta
     4801 ggtgtcttaa ttagtactgc tggaacgata aagcatattc aaaaaataga acatccatat
     4861 ccattaaatg tatttacgct aaatattgcg acttatattt ttagacatag agaagagaca
     4921 agacaatttt taacgatgca acgacagtta gctgagcagt taaaacaaat atttgataca
     4981 catgttgcag ataaaatgtc agtgttccca tcaaatgcta attttgtact tactaaaggc
     5041 tcagcagcgc aacaattagg acaatacgta tatgaacaag gatttaaacc tcgcttttat
     5101 gatgagccgg tgatgaaggg ctatgtaaga tactcaattg caacagcatc acagttaaag
     5161 caattagaag aaattgttaa agaatggagt gcaaaatatg atttatcaaa aacaacgaaa
     5221 cacagctgaa acgcaactaa atatttcaat atctgatgat cagtcaccat cgcatattaa
     5281 tacaggtgtg ggctttttaa atcatatgtt aaccttgttt acatttcata gcggtctgtc
     5341 attaaacatt gaggcacaag gtgatattga cgtagatgat caccacgtaa ctgaagatat
     5401 cggcattgtc attggccaat tgttacttga aatgattaaa gataaaaagc atttcgttcg
     5461 ttatggaacg atgtacattc caatggatga aacattagca cgtgtcgttg tggatataag
     5521 tgggcgccca tacctatcat tcaatgcatc attaagtaaa gaaaaagttg gtacgtttga
     5581 tacggagtta gtagaagaat tttttagagc ggtcgtaatc aatgcaagat taacaacgca
     5641 tattgattta attcgtggag gtaatacaca ccatgaaatt gaagctatat tcaaagcgtt
     5701 ttcccgtgca ttaggcatag cgctaactgc aactgatgat cagcgtgtgc cgtcatcgaa
     5761 aggtgtgatt gaatgattgt catcgttgat tatggattag ggaatattag taatgtaaaa
     5821 cgcgctattg aacatttagg gtatgaggtg gttgtctcaa atacctcaaa aataatcgat
     5881 caagcagaaa caatcatatt gcccggtgtc ggccatttta aagatgcgat gtcagagata
     5941 aaacgattaa atctcaatgc aatattggct aagaatactg ataagaagat gattggtatt
     6001 tgtttaggca tgcaattaat gtatgagcat agtgatgaag gcgatgcatc tggattaggg
     6061 tttatcccag gaaatatttc gcgtatccaa acagaatacc cagtgccaca cttaggctgg
     6121 aataatttag tgagtaagca ccctatgtta aatcaagatg tttatttcgt acattcttat
     6181 caagcgccga tgtcagaaaa tgtaattgca tatgcgcagt atggggctga tattccggca
     6241 attgttcaat ttaacaatta tattggtatt caattccatc ctgaaaaaag cggtacatat
     6301 gggttacaaa ttttgcgtca ggcaatacaa gggggattta taaatgattg aattatggcc
     6361 agcgattgat ttgattgggt caacaagtgt gaggttaaca gagggtaaat atgatagtga
     6421 agaaaaaatg tcacgctcgg ctgaagaaag tattgcttac tatagtcaat ttgaatgtgt
     6481 gaatcgtatt catatcgtcg acttgatagg tgctaaggca cagcatgccc gagagtttga
     6541 ttatattaag tcattgagga gattaacaac caaagatatt gaagtaggtg gtggcattcg
     6601 tacgaagtca caaatcatgg actactttgc cgcagggatt aattattgca tagttggaac
     6661 gaaaggtatt caagatactg attggcttaa agagatggca catacatttc caggtcgcat
     6721 ttatttatct gttgatgcct atggagaaga tattaaagtg aacggatggg aagaggacac
     6781 agagttaaat ttatttagtt ttgtgagacg gttatcggat atacctcttg gcggcattat
     6841 atatactgat attgctaaag atggcaaaat gtccggacct aactttgaat taactggtca
     6901 attagtaaag gcaacgacga ttcctgtcat tgcttccggt ggtattagac atcagcaaga
     6961 tattcaacga ttagcgtcgc taaatgttca cgctgctatt ataggaaagg ctgcacatca
     7021 agcatctttt tgggaggggc taaaatgatt aaaaaacgta tcattccatg tttagatgtc
     7081 aaagatggtc gtgtcgttaa agggattcaa tttaaaggat taagggatat tgggaatcct
     7141 gttgatttag caatgtatta caatgaagcg ggtgctgatg aattagtatt tttagacatc
     7201 tctaagacgg aagagggtca tagcttaatg ctagaagtga ttgaacagac agcgtcacgc
     7261 ttgtttatcc ctcttactgt agggggtggg attcaaagtc tcgatgatat tacccaattg
     7321 ctaaatcatg gtgcagataa agtatcatta aattcaagtg ctttaaaaaa tccacagctc
     7381 attaaacaag cgagtgataa attcggtaga caatgcatct gcatagcaat tgatagctat
     7441 tatgatcctg aaagaaaagc acattattgt tgtacgactg gtggtaaaaa aatgacaaat
     7501 attaaagtat atgactgggt acagcaagta gaacagttag gtgcaggtga gctcctcgtt
     7561 acaagtatgg gacatgatgg tatgaaacaa ggctttgata ttgaacacct agcaaatatt
     7621 aagtctcttg taaatattcc aatcattgct tctggtggtg gtggcaatgc acaacacttt
     7681 gtagaattat ttgatcagac ggatgtttct gcaggtttag ctgcaagtat attacatgat
     7741 cgagaaacga cggttcaatc tattaaagaa gtgatacggc aagggggtat agcagtaaga
     7801 tgaccaaata taaaattgat tttagcaaag gtttagtgcc agcaatttta caagataatc
     7861 aaacaaaaca agtattgatg ttgggttata tgaaccaaga agcttttgat aaaacgatag
     7921 aagatggtgt ggtatgtttc tattcgcggt cgaaacaacg gctatggaca aaaggtgaaa
     7981 catctggtca tacgcaacgt gttaaagata ttcatgtaga ttgcgacaat gacactattt
     8041 taattgatgt cataccaaat ggaccaacat gtcatacagg cagtcaaagt tgtttcaaca
     8101 cagaagttcc attttcagtg caaacattag cgcagacagt tcaagatagt gcccaatcca
     8161 ataatgaaaa gtcatataca aaatatttat taacagaagg tatagaaaag attacaaaaa
     8221 aatacggtga agaagctttt gaagtcgtaa ttgaagcaat taaaggtgac aaaaaagcat
     8281 ttgtaagtga agtagcagat gaactttatc atttatttgt cttgatgcat gcgcttggcg
     8341 tcgatttttc agaaattgag gcggaattag cgcgtagaca tcataagcgc aataacttta
     8401 aaggtgaacg acaaaatatc gaacagtggt aaagcaagta tggactaaga tataaggaaa
     8461 aggatcatgg cttatacact tacaaatatt gtggaaaacg tgacattttc aagtttaaaa
     8521 tacgacacca acatatttta actatgaatg ctgtgatggt actaaagttg cgaactcgtt
     8581 atagataagt agtggataat cacaatacga aatcaaaaat aattataaaa agtaaattga
     8641 gcaactcagg aatagatgtc actgttaaag atgtcgaaaa gtatatgaat cgatataatg
     8701 aagttatgaa gggaaaaaat ggcgaaaaag ctaaagagtt atgtttgtcg ttactaccta
     8761 ttaatatcat agttgtcttt acattctt
//
LOCUS       DANJFZ010000107         8660 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.107, whole genome shotgun sequence.
ACCESSION   DANJFZ010000107 DANJFZ010000000
VERSION     DANJFZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.107"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..90
                     /locus_tag="PWN45_002199"
     CDS             <1..90
                     /locus_tag="PWN45_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623944.1"
                     /translation="IHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            157..552
                     /locus_tag="PWN45_002200"
     CDS             157..552
                     /locus_tag="PWN45_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623945.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            690..989
                     /locus_tag="PWN45_002201"
     CDS             690..989
                     /locus_tag="PWN45_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623946.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            1069..1611
                     /locus_tag="PWN45_002202"
     CDS             1069..1611
                     /locus_tag="PWN45_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0623947.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(1707..2273)
                     /locus_tag="PWN45_002203"
     CDS             complement(1707..2273)
                     /locus_tag="PWN45_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL0623948.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(2275..2733)
                     /locus_tag="PWN45_002204"
     CDS             complement(2275..2733)
                     /locus_tag="PWN45_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL0623949.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(2736..3419)
                     /locus_tag="PWN45_002205"
     CDS             complement(2736..3419)
                     /locus_tag="PWN45_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623950.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(3591..4466)
                     /locus_tag="PWN45_002206"
     CDS             complement(3591..4466)
                     /locus_tag="PWN45_002206"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL0623951.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            4679..6310
                     /locus_tag="PWN45_002207"
     CDS             4679..6310
                     /locus_tag="PWN45_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDL0623952.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            6307..7980
                     /locus_tag="PWN45_002208"
     CDS             6307..7980
                     /locus_tag="PWN45_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDL0623953.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8107..8550)
                     /gene="mgrA"
                     /locus_tag="PWN45_002209"
     CDS             complement(8107..8550)
                     /gene="mgrA"
                     /locus_tag="PWN45_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDL0623954.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         2923 a         1293 c         1469 g         2975 t
ORIGIN      
        1 attcataaaa ataatttgcc aatgatatct ttgaataaag atttaggtta tcaagtgagt
       61 catgtgaaaa tgtataaaga tattgattag aattaggatt atgttgctaa ttcatgttaa
      121 aattaaaaaa gatttaatga cgttaaggag ttttatatga agaaattaat catcagtatt
      181 atggcggtca tgctattttt aacaggttgt ggtaaaagtc aagagaaagc cactctggaa
      241 aaggatatcg ataatttaca aaaagaaaat aaagaattaa aagacaaaaa agaaaagctt
      301 caacaagaaa aagaaaaatt agcagataag caaaaagacc ttgaaaaaga agtgaaagat
      361 ttaaaacctt caaaagaaga taacaaggat gataaaaaag acgaagacaa aaataaagac
      421 aaagataaag ataaagaggc atcacaagat aagcaatcaa aagatcaaac taagtcatcg
      481 gataaagata atcacaaaaa gcctacatca gcagataaag atcaaaaagc taatgacaaa
      541 caccaatcat aatcgaattg cttacttgtt atagatgaaa ggtacagcgt tttaaacctt
      601 attttaaggg tatgtattaa ttaaaatgtg gtcatgattg aaacagaacg taaaaataga
      661 caacgtaatt aataaaggag agaaacggca tgcatgaaca agattttaga attttagagg
      721 gtcaagatat tactttgcca gaattaggta gagaattaga aaatattaca ggacatacga
      781 ttgctgattc tactggcgaa attaagcgtg taattgcaca tttaccaaac tttgagtccg
      841 atacagatac ttttgttgct acatatcgtt taaaccatca acaagatttt atagatgcaa
      901 cttttactgc gctgaaatca gatagagcac gtttaaaaga agtgccagtt catgttgaac
      961 ttataagtta tatttctaaa tcaaaataaa ctgctatcta aaacgcaaag ttgatcaaaa
     1021 tatcgatttt gtgtttttta ttgagaaatt atataggagt gtcaatcgat gatttattgt
     1081 gaaacagagc gtttaatatt aagagactgg catgaagatg atctgttacc ttttcaaaaa
     1141 atgaatgcga attatgacgt acgtaaatat tttccaagtt tattgagtta tcgtcgttca
     1201 gaattagata tgagaactat ggatgcggtt attaaagatt atggcattgg attatttgct
     1261 gtagaagata aagagtccca tcaatggata ggctttatag gtttgaatta tattccagaa
     1321 acaagcgatt atccatttaa agaattaccg ctttatgaaa taggttggcg cttgttgcca
     1381 gaattttggg gaaaaggatt agcaactgaa ggcgcaaagg caacattgaa gttagcagaa
     1441 gaacatcaaa tatacgatgt ctatagtttt acagcagaag caaataaagc ttcacaacgt
     1501 gtaatggaaa aaattggcat gacagtgtat gatcatttcg aattacccaa tctaagtaag
     1561 tatcatttat taaaaaggca agtgcgctat tacattaatc ttcgaaagtg aaaaatttat
     1621 acataagcgt aacaaacacc cctaacattg tttagctgat gataacaata ttaggggtgt
     1681 ttgtttattt tttaacctta gaatgattaa tcgtatgaac gagtacccag aggtttgaaa
     1741 tttaatattg attcaattaa tgattcctta gtgtcgcata acggtgcaag agcacgatac
     1801 ttaggatcaa taaaaccttc ctcaatcata tggtcaatca ttgtttgtag tggattgaaa
     1861 aagccattaa tattataaat ggcaataggc ttttcatgga tacctatttg agcccaacta
     1921 tacatttcga aaaattcttc tagtgaacct gcgccaccag gagccatgac aaatgcatct
     1981 gcaagttctg ccattttatt tttacgttca tgcatagaat caactaaaat taattcagtt
     2041 aaacgttggc ttgtgatttc atgttcatct aacattttag gcatgacgcc aatagctttg
     2101 ccgccatgat ctaatacacc atcttgaatg gcacccataa tgccaattga ccctgcacca
     2161 aatactaatt cataaccttg ttcagcaaaa tatttaccta aatcgtatgc tttttgtaca
     2221 tatgaagggt catgaccttt gcttgcacca caataaactg cgattcgttt catgttaatc
     2281 cagctcctta attcgatgaa tgacttttaa tagtgattgt tcaaacactt tttgatcttg
     2341 ctttgtaaaa ggtgggggac ctttgtggcg accaccttgt tttctaattt gtgcattcat
     2401 atatcgttta tctaatagtt gttgaatatt tttggaattg tatatcttcc cattatgatg
     2461 catgacaatt aagactttgt cgactaataa acttgcgagt ccataatctt gagtgactac
     2521 gatatcatcc ttcgttgata attgaacaat tttgtaatca actgcatctg gtccatcatc
     2581 aacatataat gttgatacat gtggaggata taattggttc gaaaaatggc tgaagctccg
     2641 aataattgtc acaaaaatgc ctgtctcagt tgttaaatct ataatagaat caacaacagg
     2701 acaagcatct ccatcaataa taatatgtgt cacaattatg cctctgtatt gttttcttta
     2761 ttttgttgag aggcgctttt ggcaacataa tctttatatt ttttaaatga cttgatgcgt
     2821 gctttatcag cttcttgttg gcgtttttgt tcttctttgt gtcgtttttc aatatttttt
     2881 tgtaactttt tattcatttt agcgatttct ttgcgatttt tttcagctag tttatcgcct
     2941 tttttctcag ttttctcatc taatttatta ggtgttaagc ctgctttttc ttcgtatttt
     3001 tgtgattttt tcatatcttt aatacgttgt atttcattct tttcgcgggc tttttgctct
     3061 tctttatgac gcttttcgat atttttttga agtattttat tcattttatc agcgtcttta
     3121 cgattttgtt tagctaattt ttcgcctttt ttctcaatat aggcaggatc atgttctcta
     3181 gcaaactttt taagttcacg tttattttca aaatcttgtt ttttatcgcc gacatattct
     3241 ttaacatcac tcgctgtgtt actgattgct gcagatgttt ttgaagcaac tttacttgta
     3301 gcatctgtaa ctttttgtac gtccggatgt tgtttgatac gtttacgttc aacaattaac
     3361 ggtaccaata caattggtaa tacattaatc ataaatttga tgactttttt cttatccata
     3421 gatcttgcct ccataattac tttattaatt ttacataccc tatgatacat caatataaac
     3481 gatgatagta gtgaatcact attaagtatt tcagatgttt tttaaaagaa gacaataaaa
     3541 actgccaatc aagtgattcc ttaattgaca gtctatattt taaaaggaaa ttaaatacct
     3601 ttaccaatgc caaatccgaa gtaaagtata gcaataaaga ttactaatac aattctgtaa
     3661 atggcaaatg gaattagttt gattttgtta attagatgca agaatgtttt gattgcaatt
     3721 agtccaacag taaatgcagc taaaaagcct aaaatataaa aaggtatatc agcaatctga
     3781 atatcttgat aatgttttaa taaagataaa ccactagctg ctaacataat tggaacagcc
     3841 ataataaatg taaagtccga tgctgcttta tgatttaatt tcattaatac cccagttgaa
     3901 attgttgagc ctgaacggct gaaaccaggc cacatagcta ctgcttgaga aataccaatt
     3961 acaaatgctt ggaaataact gatttgatct actgtttgtg ggtttttaac tttagctgag
     4021 tatttatcag caataatcat atagatagca cctacgaata agccaatcat aacagttggc
     4081 acactaaata aatgttcttc gatgaaatca tcaaatagta agcctaaaat acctgctggc
     4141 accataccca ctaatacatg taataaattt aaacgtcttg gctttgaacg tctttgttga
     4201 tcgttatctc cttcaacatg tttgtgttta ccaatatgta aaatctctaa gaagcgttcg
     4261 cggaacaccc atgctgctgc aaagacggat cctaattgga tgacgatttt aaatgtaaat
     4321 gctgactgag aacctaaaaa ttcagatgat tttaaccaca tatcatcaac taggatcata
     4381 tgtccagtag aggaaacagg tgcaaattct gttaatcctt cgacgacccc taagataata
     4441 ccttttatta attcaatgat aaacataatg tacccacttt cattactcaa tttaatttat
     4501 ttaaatatca aaattaccat atcatgatag catattcatt taaagacatg ctagttataa
     4561 tactagacta aagatgtata tattcatttt cttttacatg taaaactaca atattttatt
     4621 gagctattta atttgatttt aaggaaaacc ttttataata ggtttaggtg atataattgt
     4681 gaaaaaatta acaacaatac tgtttcaata taaaattttt ccggtactca tgttcttggt
     4741 cagtactggt ctcggcataa tcgttataac gcaaaatatt ttaatagcag attttttagc
     4801 taaaattata agacatcaat ttcaaggttt atggattgta ttatttattt tattaggtgt
     4861 tttactttta agagcaactg tgcaatttct aaatcaatgg ttaggtgata cattagcatt
     4921 taaagttaag catatgctta gacagcgggt tatttataaa aataatggtc atccaatcgg
     4981 tgaacaaatg actatactca cagaaaacat tgatggtcta gcaccttttt ataagagtta
     5041 tttgcctcaa gtgttcaaat caatgatggt tccgctcatc ataatcattg caatgttttt
     5101 catccatttc aataccgcat taattatgtt aataactgca ccatttattc ctttgtttta
     5161 tattattttc ggtttgaaaa cgcgagatga gtcaaaagat caaatgactt atttgaatca
     5221 atttagtcaa cggtttttaa atattgctaa aggtttagtg acgttaaagc tatttaatcg
     5281 tacagagcaa acagagaagc atatttacga cgatagtact cagtttagaa ctttaacaat
     5341 gcgcatttta cgcagtgctt ttttatcggg attaatgctc gaatttataa gtatgttagg
     5401 tattggattg gttgcattgg aagcaacgct aagcttagta gtatttcata atattgattt
     5461 taaaactgcg gcaattgcga ttattttagc gcctgaattt tataatgcaa ttaaggactt
     5521 agggcaagcg ttccatactg gaaaacaaag tgaaggtgcc agtgacgttg tgtttgagtt
     5581 tttagaacaa ccgaactata ataatgaatt tctattaaag tatgaggaaa accaaaagcc
     5641 atttattcag ttaacagaca tatcatttcg atatgatgat tctgatagat tggtattaaa
     5701 tgatttaaat ttggaaatat ttaaaggtga tcaaattgca cttgtaggtc caagcggggc
     5761 aggtaaatcc actttgacac atcttattgc aggtgtttat cagccaacaa taggtactat
     5821 aagtacaaac cagcgtgatt taaatatagg aatacttagt caacagccat atattttcag
     5881 tgcttctata aaagagaata ttacgatgtt taaagatata gaaaataata ctattgaaga
     5941 agtgctagac gaagtaggtt tattagacaa agtgcaatct ttcacaaaag gcattaacac
     6001 aataataggt gaaggaggcg aaatgttatc tggtggacag atgagacgca tagaactttg
     6061 ccgtctttta gttatgaagc cagatctcgt tatatttgat gagcctgcaa ctggtttaga
     6121 tattcaaaca gaacacatga ttcagaacgt tctgtttcaa cattttaaag atacaacgat
     6181 gattgtcatt gcacatagag ataatacaat tcgccattta caacgacgct tgtatataga
     6241 aaatggaaga ctgattgctg atgatcgcaa tatttcagta aatataacag aaaatggtga
     6301 tgacttatga aaacacgact aaaatttcaa gtagataagg atttattgtt agctatagtt
     6361 gttggtgttt gtggaagttt agttgcgctc gccatgtttt tcttaagtgg ttatatggtg
     6421 acacaaagtg cacttggtgc gccactatac gctctgatga ttttagtcgt tacagtaaaa
     6481 ttgtttgggt ttttaagagc tattactcga tacgtagagc gccttatttc tcataaagct
     6541 acatttacaa tgctacgtga tattcgggta cagtttttcg gtaaattagt aaatgtcatt
     6601 cctaatgttt accgtaaact gagttctagt gatttaattt cacgtatgat tagtcgtgtt
     6661 gaggcattac aaaatatata tttacgtgtt tattatccac cagtcgtcat cggtttgaca
     6721 gcgctagtta cagtcatagt tttggcgttc atttcaatcg gccatgcgct attgattatg
     6781 gttagcatgt tgttcacttt actcattgtt ccttggttaa gctcaaaaaa agcacgtact
     6841 ttaaagaaac atgcagctaa tgaacaggcc cgatttttaa atcattttta tgattataaa
     6901 gctggtatgg atgaactacg tcgatttaat caaattaatc attatcgaga taatttgatg
     6961 gctaaattaa atcattttga taaattacaa cttaaagagc aacgcttttt aacgatttat
     7021 gattttatat taaatattat tgctatgctt tcgatttttg gtagtttagt tctaggatta
     7081 attcaaatta atgcaggcca actaaatatt atttatatga cgagtatagt tttaatggtc
     7141 ttaactttat ttgaacaagc tgtaccaatg acaaatgtcg cgtattataa agcggatact
     7201 gaccaagcat tgcacgatat taatgaagtg atatctgtac cttctactaa tggaaaaaaa
     7261 cgtcttaatg ataagtatga tgcaacgaac atttatgaag ttaaggatgc tagttttaag
     7321 tattggaatc agcaaacgta tgtgttgtcg gatattaatt ttaatgttaa tagaggcgaa
     7381 aagattgcga ttgtgggtcc ttctggttca ggaaaaagta cattactaca aattatggca
     7441 gggttatatc aattagatag tggctctgtt cgtttcgaaa atatggatat gtttgaaata
     7501 gatgacaaag ataagtttga atcgttaaat gtcttgctac aatctcaaca attatttgat
     7561 ggtacaatac gtcaaaattt atttaccgat gaaaaagatg aagcggtgca agcaatattt
     7621 aagcaattag atttagaaca tttggcacta gaacgtcaaa ttgacttaga tggtcataca
     7681 ttatctggcg gagaaattca gcgtttagcg attacgagga tgttattaaa agatactgca
     7741 tcaacatgga ttttagatga accaacaact gcattagata aacaaaatag tttaaaagtt
     7801 atggatttaa ttgaagcaca tgcagaaaca ttaattgttg ctacacacga tttaacttta
     7861 ttgtcacgtt ttgagaccat cattgtgatg ataaatggta aaatagttga aaagggaaac
     7921 tatcaacaat tactagctaa tcaaggtgct ttatggaata tgattcaata taatgcataa
     7981 aaaaactgct tgataggcta agaaaccttt caagcagttt tttaactaac aagcttacgc
     8041 gtgctaattg tttttctatt cttaataaat tctaaacatt actttaattg tcatgacaaa
     8101 agttaattat ttttcctttg tttcatcaaa tgcatgaatg actttaccta ataagcgatt
     8161 aagttcttta acttcatctt gcgataaaga agaagctgaa gcgactttgt cagatgcatt
     8221 acttaattct ggtctaatag tttcactttt gtcagtcaag tgaataaata cttcacgttg
     8281 atcgacttcg gaacgttcac gcttaattaa gtctacttgt tccattcgtt ttaataatgg
     8341 tgatactgta ccagtatcga gtgctaattc agttacgact ttcttgacgt ttacaggaga
     8401 ttcatcccat aaaattgtta agacaagaaa ttgtgggtat gttagattgt acttcttaaa
     8461 aactttgtta gagtagtagc gattaacttg tctttgagca ttgtacaaac taaagcatag
     8521 ctgttctttt aaattatgtt gatcagacat taaagttctc ctccagacat actatccgtt
     8581 tttttctctt ttcggattgg taatcattaa aaagttgatt gtttattaat tcacaacttt
     8641 ctttgattca atgccatgct
//
LOCUS       DANJFZ010000108         8636 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.108, whole genome shotgun sequence.
ACCESSION   DANJFZ010000108 DANJFZ010000000
VERSION     DANJFZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.108"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            28..297
                     /gene="rpsN"
                     /locus_tag="PWN45_002210"
     CDS             28..297
                     /gene="rpsN"
                     /locus_tag="PWN45_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0623955.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            454..1431
                     /gene="guaC"
                     /locus_tag="PWN45_002211"
     CDS             454..1431
                     /gene="guaC"
                     /locus_tag="PWN45_002211"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL0623956.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1453..2469
                     /locus_tag="PWN45_002212"
     CDS             1453..2469
                     /locus_tag="PWN45_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDL0623957.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2849..3472)
                     /gene="lexA"
                     /locus_tag="PWN45_002213"
     CDS             complement(2849..3472)
                     /gene="lexA"
                     /locus_tag="PWN45_002213"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL0623958.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3615..3848
                     /locus_tag="PWN45_002214"
     CDS             3615..3848
                     /locus_tag="PWN45_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623959.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            3984..4223
                     /locus_tag="PWN45_002215"
     CDS             3984..4223
                     /locus_tag="PWN45_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDL0623960.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            4344..6332
                     /gene="tkt"
                     /locus_tag="PWN45_002216"
     CDS             4344..6332
                     /gene="tkt"
                     /locus_tag="PWN45_002216"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL0623961.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            6610..6852
                     /locus_tag="PWN45_002217"
     CDS             6610..6852
                     /locus_tag="PWN45_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL0623962.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            6839..6937
                     /locus_tag="PWN45_002218"
     CDS             6839..6937
                     /locus_tag="PWN45_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623963.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            7031..7498
                     /locus_tag="PWN45_002219"
     CDS             7031..7498
                     /locus_tag="PWN45_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDL0623964.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            7622..>8636
                     /locus_tag="PWN45_002220"
     CDS             7622..>8636
                     /locus_tag="PWN45_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL0623965.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDM"
BASE COUNT         3183 a         1171 c         1557 g         2725 t
ORIGIN      
        1 atttgataaa aagtgaggta actatatatg gctaagaaat ctaaaatagc aaaagagaga
       61 aaaagagaag agttagtaaa taaatattac gaattacgta aagagttaaa agcaaaaggt
      121 gattacgaag cgttaagaaa attaccaaga gattcatcac ctacacgttt aactagaaga
      181 tgtaaagtaa ctggaagacc tagaggtgta ttacgtaaat ttgaaatgtc tcgtattgcg
      241 tttagagaac atgcgcacaa aggacaaatt ccaggtgtta aaaaatcaag ttggtaaaat
      301 caaatttcgt actttagccc atttacaata tcaataaaac aatgtacagt atatacgaat
      361 gctataaact gaatgttttc tcatattaat aaggaaacat tcggttttta atttgcattt
      421 aaaaataatt agtttttaaa ggggctattt aaagtgaaaa tatttgatta cgaagatatt
      481 caattaatac ctaataaatg catagttgaa agtaggtctg aatgtgatac aactatccaa
      541 tttggtccga aaaaattcaa gctacctgta gttcctgcaa atatgcaaac agttatgaat
      601 gagaaattag cgaaatggtt tgctgaaaat gattactttt atatcatgca tcgttttgat
      661 gaagaagcaa gaataccttt tataaaacat atgcaaaatt caggcttatt tgcatctatt
      721 tcagttggtg taaagaaagc ggaatttgat tttattgaaa agttagctca agaaaaatta
      781 atccccgaat atattacaat agatattgcg catggtcact cagattcagt gataaacatg
      841 attaaacata taaaaaccca tatacctgat agttttgtta ttgctggtaa tgttggtacg
      901 ccagaaggtg ttagagaatt agaaaatgct ggtgctgatg ctaccaaagt cggtataggt
      961 cctggtagag tttgtattac aaagattaaa acaggttttg gtactggtgg ttggcagtta
     1021 gcggcattaa acatatgtag taaagcagct cgtaaacctt tgattgccga tggtggtata
     1081 agaacgcatg gcgacattgc taaatcaatt agatttggtg catcaatggt catgattggt
     1141 tcattatttg cggcacacga agaatcacct ggtgaaactg tagaacttga tggtaaacag
     1201 tataaagaat attttggtag tgcatctgaa tttcaaaaag gcgaacataa aaatgtagaa
     1261 ggtaaaaaaa tgtttgtaga acataagggt tcattaatgg ataccttaaa agaaatgcaa
     1321 caagatttac aaagctcaat ttcatatgcc ggtggaaaag acttgaaatc attacgtact
     1381 gtagattatg ttattgttag aaactctatt ttcaacggtg atagagatta atatttatag
     1441 taggtgatgt aaattaaaaa attcatagta actgttgttg cgtttttatc aattattatc
     1501 attgcgccaa taacagaatt taaaccattc attcatttac aaaatgaagt aagacaatat
     1561 attgacattc acatcaataa agaaacaatt tctgcggaaa ataaattgga tacaccgaag
     1621 aaacaacaat ttgcctttaa taatatacaa atgaacatgt cgaaatcaga tgttgagaaa
     1681 acattaaata aaccaaaaag agtgacattt aatgaatatg gtacgaagtg gtatacgtat
     1741 tatgatgacg attacaataa ttttataatg ataagttaca tgaaagataa agttaatgcg
     1801 ttatatacaa atcaaaatat aatcacttca aaatcaaaaa ttaaatataa tacacctaaa
     1861 tcggttgtaa ggcaaagatt aggcgaacca gaaacagaga ttgttaaagg tagagtgcgt
     1921 tacgaacaaa ataataaaga atatgatgtt ttccataaaa atcacattta tacgacggta
     1981 ttttatgata agcatcgacg taataatgta acagctgttt tacaagtaag tgatgctatg
     2041 gaaaatagat taaaagaaca atatggagca ccatcgaaat cgcttgcaga tagttttgaa
     2101 ctacaaaatt ttgatttagt taatgctgaa agaaaacaac atcaattatc tacattgaag
     2161 tattctaaac agaattctga aactgcacgt aagcatagta aagatatggc caacaatcat
     2221 tattttgatc atacaaattt aaaaggtcaa tcaccatttg atcgattgaa aaaagatggt
     2281 attacattta actcagccgg agagaattta gcatatggtc aagttagtag tatctatgca
     2341 catcaaggat taatgaattc tattggtcac agaaaaaata ttttaaatga tacgtttaaa
     2401 atattaggtg ttggtgttga ttttaatgat gaaaaacaac ctttttggac agaaaattat
     2461 actggttaat gtttttggca tgagattaaa gggtaatgtt tgtctgtaag gaagtagata
     2521 aacatgatga caacagataa accaaaagat gcggatatac ttgaacgcgt caaagatata
     2581 cttaataaaa aagaaagaaa aaaataataa tggctcgctc ctgtaaatta ttacgggggc
     2641 gagttttaat ttgtgtaaca ttttgcagaa atctgtaatt caaaatttat attaaagaat
     2701 gagattatct aacaattcgc aatttctgtt tgaactgaat ttagattaga ataaataaaa
     2761 cctcttgaaa acgtaacttt ttcaagaggt ccaaaattta taaaaatgag ggcttaacaa
     2821 ttacgataat atataacggt ttaaaatatt acatttcgcg gtacaaacca attactttcc
     2881 caattacagc aacattgtct aggtaaattg gctccattgt actattttca ggttgtaatc
     2941 gataacgatt tttttcttta tagaagcgtt tgacagttgc ttcatcttcc tcagtcatag
     3001 caacaataat gtctccattt tctgctatgg tttgactgcg aacaattact ttgtctccgt
     3061 ctaatatacc agcctcaatc atactgtcgc ctacgacgtt taatatgaat atgtcgctat
     3121 tgtgtgtcga tgttaagtgt tcaggtaatg gaaaatattc ttcaatattt tctactgcgg
     3181 taataggaac acctgctgtg actttaccaa taactggcac atgaatcgtt tcttccatat
     3241 taatattatc atttgtttga tcacttacaa tttctatagc acgtggtttc gttggatctc
     3301 ttcttatata gcctttttct tcaagacgtg aaaggtgacc atgaacagtt gaactggatg
     3361 ctaagccaac tgcttcacca atttcgcgaa cactaggcgg ataacctttc gtttgaacaa
     3421 cttgtttaat atagttatat atttcgcttt gtcgttttgt taattctctc atatataggc
     3481 actccctaaa ttaatttaaa tacagtatat cattaatttg aaaccaaaac aaacatttgt
     3541 tcgttaaaat attgacacag aacataagtt ctgataaatt actaatacaa acaaatgttc
     3601 taggagtgaa aatgatgttt tacaataaat ataaaaacgt atcaacatat atcatcatat
     3661 ttttagtttc aagtgcagcc tttgcaatat tcttgttaag tgcgaacatt agtgctcact
     3721 cggaacaagt gtacgaaatg actgaccatc aaattaagaa caatacgata aataaagcat
     3781 acgaacataa agaccctaca aacaatagcg aacaaagaga tgggaaagtg ttcgctttaa
     3841 taaattgata cattgtcaca acgttatttt gcctattttt gcgaaatagc gttttttatt
     3901 acttttttgc tgatcttaaa tttgttatat tttgttaaag tattataatg attgaataaa
     3961 caaattgaag gtaggttttt taattgagta attctgattt gaatatcgaa agaattaacg
     4021 agttagctaa aaagaaaaaa gaagtaggat taactcaaga agaagcaaag gagcaaacag
     4081 ccttaagaaa agcttatctt gagagtttta gaaaagggtt taaacaacaa attgaaaata
     4141 ctaaagtaat tgatccagaa ggtaatgatg taacacctga aaaaattaaa gagatacaac
     4201 aaaaaagaga taataaaaat taaatcacaa atctgtaaag aattttctga cattataact
     4261 tgaaataagt attttactta tctttttatt ttaaaataag ttataatgta tttgataaaa
     4321 ttgaagaagg gaagatacac aagatgttta atgaaaaaga tcaattagct gttgatacgc
     4381 tacgtgcact aagtatcgac acaatcgaaa aagcgaattc tggtcatcca ggattaccta
     4441 tgggagctgc cccaatggct tacactttgt ggacacgtca tctgaatttt aatccacaat
     4501 ctaaagatta cttcaataga gaccgtttcg tattatctgc agggcatggt tcagcattat
     4561 tgtatagctt gttacatgtt tctggtagtt tagaattaga agaattaaag caatttagac
     4621 aatggggttc taaaacacca ggtcatcctg aatacagaca tacagatggt gtagaagtta
     4681 ctaccggacc acttggacaa ggttttgcta tgtcagtagg attagcttta gcagaagatc
     4741 acctagcagg gaaatttaat aaagaaggat ataatgttgt agatcattac acatatgtat
     4801 tagcttctga cggtgattta atggaaggta tatcgcatga agcagcttca tttgctggac
     4861 ataataaatt aagtaaatta gttgttttat acgattcaaa tgatatttca ttagatggcg
     4921 aattaaacaa agctttttct gaaaacacaa aagctcgttt tgaagcatat ggttggaatt
     4981 acttactagt taaagatggt aatgatttag aagaaattga taaagcgatt actacagcta
     5041 aatctcaaga aggaccaacg attattgaag ttaaaacaac aatcggattt ggttcaccga
     5101 ataaagcagg aactaatggt gttcatgggg cacctttagg tgaagttgaa agaaaattaa
     5161 cattcgaaaa ttacggttta gatcctgaaa aacgttttaa tgtttcagaa gaggtatacg
     5221 aaattttcca aaatactatg ttaaaacgtg ctaatgaaga tgaatctcaa tggaattcat
     5281 tattagaaaa atatgcagaa acatatcctg aattagcaga agaatttaaa ttagcgatta
     5341 gtggtaaatt gcctaaaaat tataaggatg aattaccacg ttttgaactg ggtcataatg
     5401 gtgcatctcg tgctgattct ggtactgtta ttcaagcaat cagtaaaact gtcccttcat
     5461 tctttggtgg atcagcagac cttgctggtt caaacaaatc caatgtaaat gatgcaactg
     5521 attatagttc tgaaacacct gaaggtaaaa atgtgtggtt tggtgtacgt gaatttgcta
     5581 tgggtgctgc tgtaaatggt atggctgcac atggaggttt acatccatat ggtgcaacat
     5641 tcttcgtatt tagtgattat ttaaaaccag cgttacgttt atcatcaatt atgggattaa
     5701 atgcaacgtt catcttcaca catgattcaa ttgcagtagg tgaagatggt cctactcatg
     5761 aaccaattga gcaattagct ggattaagag ccattccaaa tatgaatgtt atccgtcctg
     5821 ctgatggtaa tgaaacaaga gtagcatggg aagttgcctt agaatctgaa tctacaccta
     5881 cttcattagt attgacacgt caaaacttac cggtattaga tgtaccagaa gatgtagttg
     5941 aagaaggcgt tcgaaaaggt gcctatacag tttatggctc tgaagagaca ccagaattcc
     6001 tattattagc ttcaggttca gaagttagtc ttgcagttga agctgctaaa gatcttgaaa
     6061 aacaaggtaa atcagtacgt gttgtttcaa tgcctaactg gaatgcattt gaacaacaat
     6121 ctgaagaata taaagaatca gttattccat caagcgtaac aaaacgtgtt gcgattgaaa
     6181 tggcttcacc gcttggatgg cataaatatg taggtactgc aggtaaagtt attgctattg
     6241 acggctttgg cgcaagtgca cctggcgatt tagtagttga aaaatatgga tttacaaaag
     6301 aaaatatctt aaaccaagtt atgagcttat aagaataatt tataaagcga gtatgtttag
     6361 aagtctagga tgcataatct taggcttctt ttaaagtgtt gaaatttaga gtatagcact
     6421 taaactacat cataagtgat aagttatgaa agtatactat ttcagattaa tctttaaaag
     6481 ctctgttata acagcatgat ttttgatatt atttttagta tcgatattaa aatacttgaa
     6541 taaactagtt cttgaaataa tgtgatgaat ttagtaaaat tcagtaggat aaagaaagcg
     6601 ggtgaaacaa tggcaacttg gttagcaatt atttttatag tagctgcatt aattttaggt
     6661 ttaattggag gtttcctttt agctagaaaa tatatgatgg actacttgaa gaaaaaccca
     6721 ccaatcaacg aagaaatgct tcgtatgatg atgatgcaaa tgggtcaaaa accttctcag
     6781 aagaaaatta atcaaatgat gacgatgatg aataaaaata tggatcaaaa tatgaagagt
     6841 gcgaaaaagt aaattcgcaa ttgatagagg ctattttcca gatatggaaa tggcctcttt
     6901 ttataatcaa attaataaga ataaatatgt ttattaaaat taaagttaac aaaatgacga
     6961 atagactgag aaatgctata attcattttg tatgatttac agagagttta tttaacgaga
     7021 aggtgtctgc gtgctctatt taatattttc aatcattgta gctttattta tgggaactat
     7081 agttatagtt attcgtatga aagctcaaaa ttatccggta aatgagaaaa aaatagtttt
     7141 gccaccgttt tttatggcga ccggtgcatt gatgtacgtc gttccatatt ttaggctaac
     7201 aggatcggaa atgctagaag cctttataat tggtttgctt ttttctacag ttctaatttg
     7261 gacttctcga tttgaagtca aaggtacaga aatttatatg aaacgatcta aagcatttcc
     7321 agttattttg atttcattac ttatcattcg tactgtgatg aaaatattca ttagtaatga
     7381 aatagatcct ggagaattag gcggcatgtt ctttttatta gcattctgta tgattgttcc
     7441 ttggagagca gcaatgctat ataaatacaa aaaactaaag aaaacattaa tcaattaatt
     7501 acttttaaaa ccacttgtga tcgacttcta aatcagtcaa tgagtggttt taattttact
     7561 tgaaaaggtg aaatgattat attaaaataa aagcgaacaa atgttctata tggaggcagt
     7621 aatgaaaatt atacatacag cagactggca cttagggaaa atattaaatg gcaaacagct
     7681 tttagaagat caagcgtata ttttagatat gttcgtagaa aaaatgaaag aagaagaacc
     7741 tgatatcatt gtgatagctg gagatttata tgacacaaca tatccaagta aagatgcaat
     7801 catgttatta gaacaagcga ttggaaagtt aaatttagaa ctgcgtatac caataattat
     7861 tattagtgga aatcacgatg gtaaagagag gttaaactat ggggcgagtt ggtttgaaca
     7921 taatcagtta tttataagaa cagattttac atcgattaat tcaccaatag agataaatgg
     7981 ggttaatttt tatacactcc cttatgctac tgtgagcgaa atgaaacact actttgaaga
     8041 tgacaccatt gaaacacatc aacagggaat tacgcgctgt attgaaacaa tagcaccgga
     8101 aattgatgaa gatgccgtca atattttaat tagtcatctg actgttcaag gtggaaagac
     8161 atctgattct gaaagaccat taactattgg aacggttgaa tcagttcaga aaggtgtttt
     8221 tgatatattt gattatgtca tgctaggtca cttgcatcat ccatttagta tagaagacga
     8281 caaaattaaa tatagtggct ccttattgca gtattcattt tcggaagcgg gtcaagctaa
     8341 agggtataga cgtgtaacaa ttaatgatgg cattattaac gatgtattta ttcctcttaa
     8401 gccacttaga caattggaaa ttatctcagg cgaatataat gatgttatta atgaaaaagt
     8461 tcatgtgaaa aataaagata attatttaca ttttaaactt aaaaatatgt ctcatattac
     8521 tgatccaatg atgagtttaa aacaaattta tcctaatact ttagcgctga cgaatgaaac
     8581 ttttaattac aatgaagaaa ataatgctat agaaataagt gaaaaagatg acatgt
//
LOCUS       DANJFZ010000109         8605 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.109, whole genome shotgun sequence.
ACCESSION   DANJFZ010000109 DANJFZ010000000
VERSION     DANJFZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.109"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            177..3617
                     /locus_tag="PWN45_002221"
     CDS             177..3617
                     /locus_tag="PWN45_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDL0623966.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            3637..4515
                     /locus_tag="PWN45_002222"
     CDS             3637..4515
                     /locus_tag="PWN45_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDL0623967.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            4990..6108
                     /gene="ald"
                     /locus_tag="PWN45_002223"
     CDS             4990..6108
                     /gene="ald"
                     /locus_tag="PWN45_002223"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDL0623968.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            6203..7243
                     /gene="tdcB"
                     /locus_tag="PWN45_002224"
     CDS             6203..7243
                     /gene="tdcB"
                     /locus_tag="PWN45_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDL0623969.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            7274..8596
                     /locus_tag="PWN45_002225"
     CDS             7274..8596
                     /locus_tag="PWN45_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0623970.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
BASE COUNT         3268 a         1216 c         1517 g         2604 t
ORIGIN      
        1 tggaatgttt gaagaaaatt gattattcgt atgttttatc aagcagctat gataaaattt
       61 aaacataata caatgcgagc catttaacga tctatgttta aatggacatc gatattgtat
      121 gaattcgttg taacaagcaa gcatttctat gtgaacgaac caaaggggaa agtaacatga
      181 ttaataaaga acaattagat cttttatata aattaaaaaa agaagttgaa aagtcgcgaa
      241 atgaagcact tttacataca attaaccaag taattaagaa agtatatttg cagcaatata
      301 catgttcgtt cgttggacat ttttctgcag gtaaatcgac actgataaat ttattaattg
      361 aacaagatat cttaccaagt tcacctgtac caacgacaag taatactgct attgtgtcag
      421 tttcagacaa tcacgatatt attgctaatt tgccgaatca aacgtatgcc aaattatcta
      481 attatgatga agtaagggaa atgaatcgcc aaaatgtcga cgttgaatct gtagaaatta
      541 attttcaatc agctaaattt gaaaatgggt ttacgttgca agatacacca ggtgttgatt
      601 caaatgttgc atcacatcag tcaataacag aacaatatat gtatacaagt aatatgatat
      661 tttatacggt tgactataac cacgttcaat ctgaacttaa ctttaagttt atgaagcata
      721 taaatgatgt tggaatacct gttgtgttta tcattaatca aattgacaag catcaagacg
      781 atgaattgtc attctctacg tttaaatctc gagttgaaaa atcaattgca gattggggta
      841 ttaaattaga acgcaccttt tatgtatcta aatttgatca ccctgaaaat gaacttgaag
      901 tattatcaag ttatctaatt tcattagatc aacatagaga gacaatagag gattatacat
      961 caagaacggt tgaatacatt accgaagctc agctagatta cattcagtct gaaattcagg
     1021 aagtactaga agatttaggt atcgaagaag cggagtttga acaagcattt ttaaatagtc
     1081 aacaacatca agcaattagt gaagaggcac aacttttaaa taatccagat gaattaatgg
     1141 catttttaaa gaataagcgt aaaaatattt tagaaaatgc atacattatg ccgcataata
     1201 tgagagaaat gttacgaagt tatttggaaa gtatgtctca agactttaat gttggcggat
     1261 tttttaataa aaagaagaaa aaactacaaa ttcaacaaca gcgattatta acagcgacag
     1321 atgcgttaca agaacatgtt aatcaacaaa ttcgtcaacc aatgcgagaa gatatgtcat
     1381 ttgttacgcg ttttatcaat aaaaaagaag cttcagataa agtattaaat cagcattatg
     1441 acgttaagcc agaaatgatt gaaggtttat atcaaccaca aacatcaatc agcaatactt
     1501 atgtacttac attttcagac gaagtggtta aagccattaa gaaatatgtt gaacaacaat
     1561 caacaccaat ttttaaagaa ataatagaaa atgtgcaggc agatgaatta ccaacagaag
     1621 aaagtgatga tttaaaagaa tatcaacgtt atacagaatt aaatgagctg cgtcagtcat
     1681 tgacgactaa gaattatcgt cactactata ttcatttaga tgaatctcta gataaattaa
     1741 taggtcgaca agagacaaca tatcaagtgg ctactgataa tgctcaggat aatcgtgata
     1801 atcagcagct aaatcaaaat acagctacaa caaatatgtc tatagatatt caaaaagcgc
     1861 ttgatataat ttcggatgtg cctttgttca agcgtacaaa gcaagatatc cacgaaacat
     1921 taacacgtat agataataaa ttaataaaaa taggtgtatt tggaacattt agtgctggta
     1981 aaagtagttt gataaatgca ttattaggcg agcatatttt agtcagttct ccaaatccta
     2041 ctacagcagc tactacagaa atatcttatg gagacgagag ttatataacg ctaaaatcac
     2101 aatcgcaatt attagatgaa attaatgcag tagttgaata ccaagatatg tcttattcga
     2161 ctatagaaga ctttattaat tcagatttag aaaagttaaa atcacattta aataaaaatc
     2221 aactcgcttt tattcaggca gttgaaaaac attataaatt gtatgtcaat atgttggaaa
     2281 atggagaaaa acatgccatt aatcaacagg aattgaaaaa gtggagtgca gaagatgaat
     2341 atgcaacatt tgttaagaca gtacacattg cattaatgca tgattggtta aaaggtaaaa
     2401 taattgttga ttcattaggg ctacactcaa ataaccaaag gcatacaaat gaaaccgaac
     2461 aaattttaac ttcttcagac ttaatattgt atgtaagtta ttttaatcat tcatttactg
     2521 ataatgacaa agcgtttata gaacacatga aagatatgaa ccagttgaat gaaaaccaag
     2581 cattaaaaat ggtaattaat gctgctgatt tagcagaaag tcaagatgat cttgaagcag
     2641 ttgaaacata tgtatcagat gcattaagac aagtacactt acaatcagac atttttgctg
     2701 tatcaagtcg aaatgcattg caagctgaag ataagggcat tgatcaatta aaacaaagca
     2761 tacaacaatt tgttgatgtt gaatctaaat caattttaga acaacaaatg attcatcagc
     2821 ttcaacaaat ggatcgttct tatgtagaga tgattacaga atttgaaaca aataaagctg
     2881 atatttcacg tcgccaacaa aggttaacag attataaaga taaacagcgt ttacaacatc
     2941 aattaattga tgcgacgtta caacatacag acaacgaagt tgaagaacaa gtttatcatt
     3001 taaatgcccg tttaaaacta caactacttg acgatgttaa atcagtgttt aattctcaaa
     3061 tgacgcaaaa tagtgatttt aatgaagaaa agaaagtgtc tacgagagta tacttagatc
     3121 aaattcatca acgattgttt ttagagcaat ctttaattac agaacgtata aaaaaatact
     3181 ttaataagca actcactgag caaattgcac caatcgttca acaattagca gatttacatg
     3241 tcattattaa tcctcagttt aactttgaat cagctaatat agagcaacca ttattgcaca
     3301 tcgatttcaa cgatatgcta aatgcattgc ctaaacaatt aacaaaacgt aaaattttga
     3361 atccaaatgg gcaaagagat atacatgaat caatttgtca aagtacgtta gaattattac
     3421 aaccacaaat gggattattg aggcaacagc ttgaattata tgtaaagcaa atggctgtag
     3481 aagctgaatc gcaatttgaa agttttgaag ctaatattca aacgcaaata aacgatttat
     3541 tagcatttga tttagataca acacttatca atcaattgaa agataaacat caacaactga
     3601 aaactatttt atattaagaa agaaggaacg ttttaaatgc ctaataaaat attacttgta
     3661 gatggtatgg cgctattatt tagacatttc tatgctacaa gtcttcataa acaatttatg
     3721 tacaattcac aaggtgaacc tacaaatgga atacaaggat ttgtgcgtca tatcttttcg
     3781 gcaatacatg aaatacgccc tacacatgta gctgtatgtt gggatatggg acaatcaact
     3841 tttagaaatg atatgtttga tggttataag caaaatcgtt ctgcaccacc agaagaattg
     3901 ataccacaat ttgattatgt taaagaaatt tcagagcaat ttggctttgt aaatattggc
     3961 gttaaaaact atgaagcgga tgatgttata ggtacattag cacaacaata ttcaactgat
     4021 aacgatgtct atattattac gggcgacaaa gatttactgc aatgtattaa tgacaatgtt
     4081 gaagtttggc taattaaaaa aggttttaac atttataata gatatacatt acatcgtttt
     4141 aacgaagaat atgcccttga accacaacaa ctaatcgata ttaaagcgtt tatgggtgat
     4201 acagcagatg gatatgcggg tgttaaaggt attggcgaga aaacggctat taagttaatt
     4261 cagcaatatc aaagcgttga aaatgtggtt gaaaacattg atgcattatc agctgggcaa
     4321 cgtaataaga ttaatgacaa tttagatgaa ctatatttat cgaagcgact ggctgaaatt
     4381 cacacacaag taccaattga tagtgaagcg ctttttgaga aaatgtcatt tgcaacaacg
     4441 cttaatcata ttttatcaat ttgtaacgaa catgaattac atgtttcagg aaaatatatt
     4501 tcatcccatt tttaaaaagt ataagatttt atgaaaaatt aaaccaatta agtataaaaa
     4561 gactatttct atcgatacaa ggtagaaata gtctttttgc gtatttggat atgtcagagt
     4621 gaataaataa gccgaatttt gatagataca tatttgatta atcaatagag tataaagaat
     4681 tatatttcat ccaaataaat taccatacac tacaaaatag tacagttaca actttattaa
     4741 tctaaaaaac atatttgatt aattagtttt tgagtcaatg tattaataca actataaatc
     4801 aaatggagta taagaaataa atatagtggt ataattgcac ataatattta agaataagaa
     4861 taatatatta aatatttatt aacaaattat agataaaata tgaataatta attaatggta
     4921 tttacatatt cataaccaaa ggataaaata attgtgaaat tattcacaaa tgaaagagga
     4981 gtgttgcaaa tgttagttgc agtagtcaaa gaattaaaac aaggtgaagg acgtgtagct
     5041 tgcacacccg aaaatgtgcg taagttaacg gatgcaggac ataaagtaat tgttgaaaaa
     5101 aatgctggca ttggttcagg attttctaac gatatgtatg aaaaagaagg cgctaagatc
     5161 gtaactcacg aacaagcatg ggaagctgat cttgttatca aagtaaaaga acctcatgaa
     5221 agcgaatatc aatatttcaa aaagaatcaa attatctggg gatttttaca tctagcatct
     5281 tcaaaagaaa tagtagaaaa aatgcaagaa gttggtgtaa ctgcgattag tggtgaaacc
     5341 attataaaaa atggaaaagc agaattatta gcgccaatga gtgctatagc aggtcaacgc
     5401 tcagcaatta tgggagctta ctactctgaa gcacaacatg gtggtcaagg tactttagtg
     5461 actggtgtac atgaaaatgt ggatatacct ggtagtacat atgtgatttt cggtggtgga
     5521 gtagcagcaa caaatgcagc aaatgttgcc ttgggactaa atgctaaagt aatcattatc
     5581 gagttaaacg atgaccgcat taaatatctt gaagatatgt atgcagaaaa agatgtcaca
     5641 gtagtcaaat caacaccaga aaatttagca gaacaaatta agaaagcaga tgtatttatt
     5701 tctacaattt taattccagg tgcgaaaccg ccaaaattgg ttactcgtga gatggttaaa
     5761 tcaatgaaaa aaggttcagt attaatcgat atagctattg accaaggtgg aactattgaa
     5821 acaattagac caactacaat ttctgatcca gtgtatgaag aagaaggtgt gattcattat
     5881 ggtgtaccaa atcaaccagg agcagtccca agaacttcaa caatggcatt agcacaagga
     5941 aatattgatt atatattaga aatttgtgac aaaggcttag aacaagcaat taaagataat
     6001 gaagccttaa gtactggtgt aaacatttac caaggacaag tgacaaatca aggattagct
     6061 tcatcacatg acctagatta taaagaaata ttaaatgtta tcgaatagat agtaatttaa
     6121 atgaaattga gtgaaatgaa tattttaaat atagcattat agtttggact aaaaatttac
     6181 aaaacggaag gatgtaatta ttatgacaac caacacagtt acattacaaa cagcacatat
     6241 tgtaagttta ggagatatcg aagaagctaa agcaagcatt aaaccattta ttcgtcgaac
     6301 acctctaatt aaatcaatgt atttaagcca aagtataact aaagggaatg tatttctaaa
     6361 attagaaaat atgcaattca caggatcttt taaatttaga ggcgctagca ataaaattaa
     6421 tcacttaaca gatgaacaaa aagaaaaagg cattatcgca gcatctgctg ggaaccatgc
     6481 acaaggtgtt gctttaacag ctaaattatt aggcattgat gcaacgattg taatgcctga
     6541 aacagcacca caagcgaaac aacaagcaac aaaaggctat ggggcaaagg ttattttaaa
     6601 aggtaaaaac tttaacgaaa ctagacttta tatggaagaa ttagcgaaag aaaatggcat
     6661 gacaatcgtt catccatatg acgataagtt tgtaatggca ggccaaggaa caattggttt
     6721 agaaatttta gatgatattt ggaatgtgaa tacagtcatc gtaccagttg gcggtggagg
     6781 attaattgca ggtattgcca ccgcattaaa atcatttaac ccttcaattc atattatcgg
     6841 tgttcaatct gagaatgttc atggtatggc tgagtctttc tataagagag atttaactga
     6901 acatcgagtg gatagcacaa tagcagatgg ttgtgatgta aaagttcctg gtgaacaaac
     6961 atatgaagta gttaaacatt tagtagatga atttattctt gttactgaag aagaaattga
     7021 acatgctatg aaagatttaa tgcagcgtgc caaaattatt actgaaggtg caggcgcatt
     7081 accaacagct gcaattttaa gtggaaaaat aaacaataaa tggcttgaag ataaaaatgt
     7141 tgttgcatta gtttcaggcg ggaatgttga cttaactaga gtttcaggtg tcattgaaca
     7201 tggactgaat attgcagata caagcaaggg tgtggtaggt taaaacattt aatcttaaaa
     7261 atgaggtgta attatgtcaa atggtaaaga attacaaaaa aatataggtt tcttctcagc
     7321 gtttgctatt gttatgggga cagttattgg ttcaggagta ttctttaaaa tatcaaacgt
     7381 aacagaagta acaggaacag caggaatggc cttgtttgta tggttcctag gcggcatcat
     7441 taccatttgt gcggggttaa cagcagcaga acttgctgct gcaatccctg aaacaggtgg
     7501 cttaacgaag tatatagaat atacatacgg tgatttctgg ggcttcctat caggttgggc
     7561 gcaatcattt atttattttc cagctaacgt agcagcattg tctatcgtat ttgcgacaca
     7621 gctaattaat ttattccatt tatctatagg ttcgttaata ccaatagcaa tcgcatctgc
     7681 gttatctatt gtgttgataa atttcctagg ttcaaaagca ggcggaattt tacaatcagt
     7741 tactttagta attaaactga ttccaatcat cgttattgta atttttggta tttttcaatc
     7801 tggagatatc actttttcat taattccaac tacaggtaat tcaggaaatg gcttctttac
     7861 agcaattggt agtggtttat tagcaactat gtttgcatat gatggttgga ttcatgtagg
     7921 aaatgttgcg ggggaactta aaaatcctaa acgcgattta cctttagcga tttcagttgg
     7981 tatcggttgt attatggctg tgtatttatt aattaacgct acattcttat taacgttgcc
     8041 aatagaacta cttgctggta atttaaatgc agcttcagat acatcaaaaa tattatttgg
     8101 tgaaaatggc ggtaagatta ttacaatcgg tatattaatt tctgtttatg gtacgatcaa
     8161 tggctatact atgactggta tgcgcgtacc atatgcaatg gctgaaagaa aattattgcc
     8221 atttagccat ttattcgcaa aattaacaaa atctggcgca ccatggtttg gcgcaattat
     8281 acaacttata atcgctatca tcatgatgtc aatgggagca tttgatacaa ttacaaatat
     8341 gttaatcttt gttatttggt tgttctattg tatgtcattt gttgcggtaa taattttaag
     8401 aaaacgtgaa ccaaatatgg aacgaccata taaagtaccg ttatatccga tcataccttt
     8461 aattgctatt ttggcaggat catttgtatt aattaataca ctgtttacac aatttatatt
     8521 agcaatcatt ggaattctaa taacagcact tggtatacca gtttattact ataaaaagaa
     8581 acaaaaagca gcataaggta agata
//
LOCUS       DANJFZ010000110         8504 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.110, whole genome shotgun sequence.
ACCESSION   DANJFZ010000110 DANJFZ010000000
VERSION     DANJFZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.110"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            100..513
                     /locus_tag="PWN45_002226"
     CDS             100..513
                     /locus_tag="PWN45_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDL0623971.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            713..1156
                     /locus_tag="PWN45_002227"
     CDS             713..1156
                     /locus_tag="PWN45_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL0623972.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(1277..1987)
                     /gene="deoD"
                     /locus_tag="PWN45_002228"
     CDS             complement(1277..1987)
                     /gene="deoD"
                     /locus_tag="PWN45_002228"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0623973.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            2302..2964
                     /gene="deoC"
                     /locus_tag="PWN45_002229"
     CDS             2302..2964
                     /gene="deoC"
                     /locus_tag="PWN45_002229"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL0623974.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            3243..4544
                     /locus_tag="PWN45_002230"
     CDS             3243..4544
                     /locus_tag="PWN45_002230"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL0623975.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            4558..4908
                     /locus_tag="PWN45_002231"
     CDS             4558..4908
                     /locus_tag="PWN45_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623976.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(5060..5530)
                     /locus_tag="PWN45_002232"
     CDS             complement(5060..5530)
                     /locus_tag="PWN45_002232"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL0623977.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            5841..7025
                     /locus_tag="PWN45_002233"
     CDS             5841..7025
                     /locus_tag="PWN45_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL0623978.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            7025..8218
                     /locus_tag="PWN45_002234"
     CDS             7025..8218
                     /locus_tag="PWN45_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL0623979.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
BASE COUNT         2982 a         1203 c         1671 g         2648 t
ORIGIN      
        1 caatttcgtg tcgcgaggac ttatcaagtg acaaccttgt ttaaatgttt ttaaattagg
       61 aaaaatgact ataagtacta ttaaaggagc gaataaatta tgccaatcgt atattatgat
      121 ggaaactgta tatattgtta taactatgtc atttggttaa ttcaacatga cctaccaaga
      181 aattatcaat ttgccacgtt aaaaggtgaa gttggccaac aattttttaa acaacatcca
      241 gaagcggcaa ataaaaatag tgtgatttta cagaaaggtg atcgtatata ttttgaatcg
      301 caggcgatta ttaaactaat cacagcacta cctaatcgaa ctaaattatt gggcgtaggt
      361 ttatggcttg tacctaaacc agtccgtaat ttcggttatc gaatgtttgc taataataga
      421 aatagaatgt ggaaaacaac atggcaccaa ccaaatgatt atgaaaaatc gttcttttta
      481 gatgataatg cgaaagtaaa acttactgat tgataaaaca tacttgctaa ttgataatgg
      541 atatattaga tgatgaatta aaatttaggc atttaaatag cggaacactt tacggttaga
      601 ttagaataat tataaaaaag agagcaaaaa cactttacag attagaatta ttataatata
      661 ataattaata taaacaagca agacgtagac aattttaagg agtgtattaa atatgagtaa
      721 tcaacaagat gttgtaaaag aattgaatca acaagtagca aactggacag tagcttacac
      781 aaagctacac aatttccact ggtatgtgaa agggcctaac ttcttctcat tacacgttaa
      841 atttgaagaa ttatataatg aagcaagcca atatgtagac gaattagctg aaagaatttt
      901 agcggtagga ggaaaccctg taggtacatt aactgaatgc ttagagcaat caattgttaa
      961 agaagctgcg aaaggttact ctgcagaaca aatggttgaa gaattatcac aagacttcac
     1021 aaatatctca aaacaattag aaaacgcaat cgaaattgct ggtaatgctg gcgatgatgt
     1081 atcagaagat atgtttatag gtatgcaaac atcagtagat aaacataatt ggatgtttaa
     1141 atcttactta agctaatcta cagataagta ataatataaa taaaagccaa cacataaatg
     1201 gggatacgcc cagaatgtgt tggctttttg agtttaatcg cattgttaaa gagcaattaa
     1261 agtcaattta aataagttac tctgcgattt ctaaagcgat ttccatcatt tgtgtaaatg
     1321 aattttgacg ttcttcaggt gtagtagctt cgtcacgtaa aatatgatca cttactgtga
     1381 aaataccaag tgctttttta ccagcatgaa tcgcatttaa atataaacca gctgattcca
     1441 tttcgatacc taaaataccc atttttttcc aagcatcatt gaatgttgga tcggcattgt
     1501 aaaatgtatc agaagataat acgttaccta cgtgtgtagt agcaccgatt tggtcagcga
     1561 catttttagc tttagttact aactcgaagt cagcgatagg cgcgaaatga cctggaatat
     1621 tgtattgatc tacataattt gaattagttg atgcagcttg tgcaataata acatcgtata
     1681 agttaacatt ttcttgtaat gcgccacaag aaccgatacg aatgattgta tctacatcaa
     1741 agaagttgta taactcatat gagtaaatac caatacttgg aatacccata ccagaaccca
     1801 ttacagaaac ttctttacct ttatatgtac cagtgtaacc aaacatgtta cgtacatcgt
     1861 taaattgttc aacattttct aaaaaattat cagcaatata ttttgcacgt agcggatcgc
     1921 caggcattaa tactgtttta gcaattttta ctccatttgg ttgaatatgt ggtgtacctt
     1981 ttgtcatttg ttatcttctc cttaatattc attaataatt ttaagataac atttgtttcc
     2041 cttttttgcg aatttttgta ctatagaatt aaagaattat aaaaatataa tcctgattag
     2101 cttgttgtca agtcatcgtt cataatgaag atatcacgtt caattgtatt gtttgtttat
     2161 gggaaatgaa ttaatgtaat agtatatgta tgcggttaca taaaaagcga acatctaacc
     2221 tgatatttaa atgaacctga cgctcaatca actaatttac aaccgtattt ttataatcaa
     2281 ccataaagga ggagatagaa aatgaatagt gcaaaattga ttgatcacac tttattgaag
     2341 cctgagtcaa cacgtacgca aatcgatcaa atcatcgatg aagcgaaagc ataccatttt
     2401 aaatctgtat gtgtgaatcc aacgcatgtt aaatatgcag cagagcgact agctgattca
     2461 gaggtgttag tttgtacggt aataggattc ccattaggtg catcgacaac tgcgacgaaa
     2521 gcatttgaaa cagaagatgc gattcaaaat ggtgcagatg aaattgacat ggtcatcaac
     2581 atcggcgcat taaaagatgg acgttttgat gatgtacaac aagacattga agcagtggtg
     2641 aaagctgcga aaggtcacac agtaaaagtg attattgaga cggtattgtt ggaccatgac
     2701 gaaattgtaa aagcgagtga attaacaaaa acggctggtg cggacttcgt taaaacttca
     2761 acaggttttg caggtggcgg tgcgactgca gaagacgtta aattaatgaa agatacagta
     2821 ggtgctgatg tagaagtaaa agcatcaggt ggcgtacgta atttagaaga tttcaataaa
     2881 atggttgaag caggtgcgac acgtattggt gcgagcgcag gcgttcaaat tatgcaaggt
     2941 ttagaagcag attcagatta ctaattttat aaaaatatgt aagcatggga catcgtaacc
     3001 atatataagt atgtcgatat cagctttaag acatcataaa tgccatgatg attctaagat
     3061 aatttaatag ggactttaac gttataacca atctatttaa gaataataac atcgcattga
     3121 aattgtgata ttaaaaatga aatcgatagc ttgctataat aattttcgct tgtgctttta
     3181 aaaatggact agagaaagcg cagatgcttt gtcctaagct ttttaagaaa aggatgataa
     3241 aaatgagaat gatagacatt attgagaaaa agcgtgacgg tcatacacta acaacggaag
     3301 aaattaattt ctttattggc ggctatgtta aaggggatat tcctgattac caagcatcaa
     3361 gtttagcaat ggcgatttat ttccaagata tgaatgatga tgagcgtgca gcattaacga
     3421 tggctatggt taattctggt gatatgatag atttgagtga tattaaaggt gtcaaagtag
     3481 ataagcactc aacaggtggt gtaggagata caactacatt ggttttagca ccattagtag
     3541 cagctgtaga tgttcctgtt gcaaaaatga gtgggcgtgg attaggtcat acaggtggta
     3601 cgattgataa attagaagca attgatggtt ttcatgttga aatagatgaa gcaacatttg
     3661 tgaaattggt taatgaaaat aaagtggcag ttgtaggaca atcaggaaat ttaactcctg
     3721 cagacaaaaa attatatgcc ttaagggatg ttactggtac tgtcaattca ataccattaa
     3781 ttgcctcttc aattatgagt aaaaagattg ctgctggtgc agatgcaatt gtattagatg
     3841 taaaaactgg tagcggtgca tttatgaaaa cattagaaga tgctgaagca ttagcgcacg
     3901 caatggtacg tattggtaat aatgtgggac gtaatacgat ggcgattatt tctgatatga
     3961 atcagccact tggacgtgcg attggcaatg cgcttgagtt acaagaagca attgatacgt
     4021 tgaaaggaca aggtccgaaa gatttaacag aacttgtatt aacattaggt tctcaaatgg
     4081 ttgtacttgc aaacaaagct gaaacgcttg aagaagcgag agcgttgcta attgaggcaa
     4141 ttaattcagg tgcagcatta gaaaaattca aaacatttat taaaaaccaa ggtggcgacg
     4201 aaactgttat tgaccatcca gagcgtttgc cacaagctca atatcaaatt gaatataaag
     4261 ctaaaaaatc aggttatgtg actgaattag tctctaacga tataggtgtc gcttcgatga
     4321 tgttaggagc gggacgttta acaaaagagg atgatattga tttagcggtt ggtattgttt
     4381 taaataaaaa aattggtgat aaagtagaag aaggagaatc attattaacg attcacagta
     4441 atcgccaaga tgtagacgat gttgttaaaa aattagattc aagcataaca attgctgatc
     4501 atgttgtatc gcctacatta attcataaaa tcattacaga ataggagttg aagctgattg
     4561 aacatactaa gatatttatt aggaataggt tttagtgtaa taggtgtatt gcattttaca
     4621 cgagaacgac aatttagaaa tatcataccg aaatgtttgc cacttcgaaa aacagcggtt
     4681 cttgttacag gaatctttga gatattattt ggacttgcac ttttaattaa aagaccatct
     4741 caatgcttga aaaatacaat caatctattc ttgctagctg tattcccagc taatatctat
     4801 atggcagtta aaaagttacc tttaggagat aaacaattgc caacatggca actatattta
     4861 aggttaccta tgcagttcgt gttaatggga ctaattaaga aattatagtg gttctcaaat
     4921 aaatcggaca aatgttaaag attttacttt ttcatttgtt cgattttttg actttgaagc
     4981 ataataaaaa gcagttggac aatggcgatt gtaaaatcat tgttcgactg cttttctgat
     5041 taacttgatt gactaagatt tattttcctg taccgaaaac atcatgccat tcgtttcgtt
     5101 tgtctagaaa tgcttgagcg atagttttag caccttctaa tgaatgactt gctgcccagc
     5161 cacattgtac ttcattacaa gcaggcactt cagtagcatt tagcacatca tttaaagttg
     5221 cttcaacaat atttaataca tcatcataat tatcatgatt aataaatgat acatagaaac
     5281 cagtttggca acccatagga cttaaatcaa caactttgtc actatgattt ctaatatttt
     5341 cagccattaa atgttctaat gaatgtagtc cgggcatatc catatgttct ttgtttggtt
     5401 gtttgaaacg aatgtcgtat ttgtgaatga catctccgtt taatccttcc atcgttcccg
     5461 ctaatctaat aaatggggca accaccttag tatgatctaa attgaaactt tcaacattca
     5521 tttttgtcat tttgaatttc ctcctattaa ctactcaaaa ctataaaacg cttatgattt
     5581 gttgcattat aaaacttaag tttgttgtaa gttgtgtttc taacaatact taaattgtaa
     5641 caatagggct tatgttttac aatttgaaac tgttttataa atttatgaaa ggcttttagt
     5701 tattagaaaa ttttgtaaat aaagaaaaac tagatgacat catgagataa aaccgataga
     5761 ataagtaata attcattttg aaagaacaaa atattgtgga tgattaaaat gagtgtaggg
     5821 taaaagggag atagtaaaaa atgagtgaaa aacaacaaat tctcgattat atagaaacaa
     5881 ataaatatag ttatatcgaa atcagtcata gaattcatga acgtcctgaa cttggtaatg
     5941 aagaaatatt tgcgtctcga actttaatag atcgtttgaa agagcatgat tttgaaattg
     6001 aaaccgagat tgctgggcat gcaactgggt ttatagcgac atatgattca ggacttgacg
     6061 ggccagctat aggtttttta gcagaatacg atgctttgcc aggattgggt catgcttgtg
     6121 gtcataatat cattggaact gcaagtgttc ttggtgcaat tggtttgaag caagtgattg
     6181 accaaattgg tggtaaagta gtcgttcttg gatgtccagc tgaagaaggt ggggaaaatg
     6241 gtagcgctaa agcttcttat gtcaaggctg gtgtgattga tcaaatagac attgccttaa
     6301 tgattcatcc gggaaatgaa acttataaaa cgattgatac tttggcagtg gatgttttag
     6361 atgttaaatt ttacggaaaa agtgctcatg cctctgaaaa tgcagatgaa gcgttaaatg
     6421 cattagacgc tatgattagt tattttaatg gtgtagcaca actacgacaa catattaaaa
     6481 aagatcaacg tgtgcatggt gtgattttag atggcgggaa agcagctaat attattccag
     6541 actatacaca tgctcgtttt tatactagag caatgacgcg taaagaattg gatatattaa
     6601 cagaaaaagt aaatcaaatc gcacgtggag ctgcgataca gactggttgt gattatgaat
     6661 ttggtcgaat tcaaaacggt gtgaatgaat tcattaaaac gccgaaatta gatgatttat
     6721 ttgctaaata tgctgaagaa gttggtgaag cagttattga tgatgatttt ggttatggct
     6781 ctacggatac agggaacgta agtcatgttg tgccaacaat acatcctcat attaaaatag
     6841 gatcacgtaa tttagtagga catacgcata gatttagaga agcggctgcg agtgtacatg
     6901 gtgatgaagc attaattaaa ggcgctaaaa taatggcgtt gatggggtta gagttaatta
     6961 caaatcaaga cgtttatcaa gacattattg aagagcatgc gcatttgaaa gggaatggga
     7021 agtaatgaca aataatggta atgagccgaa cttaacgtta agtgatttat atgacaaaga
     7081 tgttgtctat acgtcaagac cttcgtatat atcgaatcca tggttgaaac cggacgaaca
     7141 tcaatctaat tttttaactg gtagggagtt attaatagca aatcaattac ctgttattgt
     7201 tcacgaagct agtgcgacag acaaattgca tcagctattt caagttattg gtaaggaagt
     7261 accgaactca atttatacat ttaacaatca gcaatcttac gaaaacttga tcaaacaatt
     7321 ggcacataag gaaaataaaa agatttattt tcaatatatt catgatgaaa caatcttgaa
     7381 tcagcaatat tatgcattag ataaaacgtt atttgttgct ttaaataata aagcaagaat
     7441 acctgaatgg acaaatggga agtttttacc aaaaagaaaa gttgtaaaaa ttgagcaatt
     7501 cgaaaacgag attaagaatt gggaattccc gttagtaatt aagccgggag atgatcttcc
     7561 gacagcaggt ggctatggcg ttatgatttg ttatcatgat gcagatttac aaaaagcgat
     7621 aacgcgtatt aaagaggcaa ctgctgaaac gaattcgctt attatcgaac agaagattga
     7681 agaaaaagcg aattattgcg ttcagtttgc atactcggaa tcgctaggca ttcaatatct
     7741 tggtgcggca acgcaattaa cggataagta cggattttat aacggaaatg aaaatactac
     7801 taatgtacca gagcacgtta tagaagcagg tagacaaatt atggaaaacg gtgtgaatca
     7861 aggattcttt ggtgtagctg gttttgacct actcgtcgat gaggatgata acgtttatgc
     7921 gattgattta aactttagac aaaatggttc aacgagcatg ttattacttg ctaacgagtt
     7981 gaattcagga tatcaaaagt tttatagtta tcattcaaaa ggtgataaca cacatttctt
     8041 caatacgatt ttgaaatatg tcaaagaagg tagtttatac ccgttatctt attatgatgg
     8101 tgattggtac ggtgaagata aagttaaatc aaggtttggc tgtatttggc atggtgattc
     8161 aaaagaaaca gtactggaga atgaacgcgc atttttagct gaacttgaac actattagag
     8221 ttcggaacat aaggcgctac aatgttgtgt tgccagtagt tgactgaata tgcgtttgta
     8281 acaagctttt ttcgattcta gtcaacagta attaaattta tgatatggca atactttgta
     8341 atactaatat taaatggcga cttttatttc actatgttat aagagttgcc attttgttga
     8401 taaaggtata ctaaaggtta tcgttttgaa atttttagta actagatatg tttcgtgtta
     8461 tagaccgaat ttgtgtatac gtaaaattta atgctattga attt
//
LOCUS       DANJFZ010000111         8477 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.111, whole genome shotgun sequence.
ACCESSION   DANJFZ010000111 DANJFZ010000000
VERSION     DANJFZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.111"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(92..649)
                     /gene="efp"
                     /locus_tag="PWN45_002235"
     CDS             complement(92..649)
                     /gene="efp"
                     /locus_tag="PWN45_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL0623980.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(675..1736)
                     /locus_tag="PWN45_002236"
     CDS             complement(675..1736)
                     /locus_tag="PWN45_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0623981.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1841..2422
                     /locus_tag="PWN45_002237"
     CDS             1841..2422
                     /locus_tag="PWN45_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623982.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2436..2654
                     /locus_tag="PWN45_002238"
     CDS             2436..2654
                     /locus_tag="PWN45_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623983.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(2718..3548)
                     /locus_tag="PWN45_002239"
     CDS             complement(2718..3548)
                     /locus_tag="PWN45_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL0623984.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            3706..4092
                     /locus_tag="PWN45_002240"
     CDS             3706..4092
                     /locus_tag="PWN45_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0623985.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            complement(4457..5929)
                     /gene="gcvPB"
                     /locus_tag="PWN45_002241"
     CDS             complement(4457..5929)
                     /gene="gcvPB"
                     /locus_tag="PWN45_002241"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDL0623986.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5922..7268)
                     /gene="gcvPA"
                     /locus_tag="PWN45_002242"
     CDS             complement(5922..7268)
                     /gene="gcvPA"
                     /locus_tag="PWN45_002242"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDL0623987.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            complement(7288..8379)
                     /gene="gcvT"
                     /locus_tag="PWN45_002243"
     CDS             complement(7288..8379)
                     /gene="gcvT"
                     /locus_tag="PWN45_002243"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDL0623988.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
BASE COUNT         2747 a         1568 c         1163 g         2999 t
ORIGIN      
        1 cattttaggc aatagcttta tgagacttat ttagaatatc ttacgttaaa tcagtatagt
       61 gaatacaagc tatttgttaa caaattagag attatcctct tgaaatgtag cttccatcac
      121 cagtgttgat aattaaaacg tcaccttcgt ttacaaataa aggtacattt aatgtataac
      181 cagtttcaac agttgccgat ttagtggcac cagttgcagt atcaccttta ataccaggtt
      241 ctgtttcagt tactgttaat tcaacagttt taggtaattc aacaccgata gtttcacctt
      301 cgtatgtttg aatttgtact tccatacctt cttttaagta attcaattct tcttttaagt
      361 aatcacttga aagttctgtt tgttcaaagc tttcattatc cataaataca tgattatctc
      421 cgtcagcata taaatattgc atgcgacgat tttcaatcat tgctggttca actttttcac
      481 cagctctaaa cgttttctct tgaattgcac cagttcttaa attacgtaat tttgaacgaa
      541 cgaatgctga acctttacca ggctttacat gttggaagtc tataactttc caaatagcgt
      601 tatcaacaga aattgttaaa cctgttttaa aatcattaac cgaaatcatt cagtttcctc
      661 ctcattttac acgcttatgt taaaactata aggtcttttg tgcatttagt aaagacttga
      721 caaccatttt ctgtaattaa aatatcatct tctattctta taccgcccaa accttctata
      781 taaacaccag gttctactgt aacacagttg ttaacttgaa gtttatcttg tatcgtacga
      841 gccagcattg gcccttcatg gatttctaaa ccaataccat gtcctagtga atgtccaaat
      901 tcttttccat acccttttga ctctaaatag tttcttgaaa tggcatcagc ttctgcacca
      961 gtcatgccag gtctaatctc attaattgct ttcatttgag attcaagtac tatttgatat
     1021 atttctttca gtttaggatc tggttctcca atagcaaatg ttctagtaat atctgaacaa
     1081 tagccgttat aatacgcgcc aaaatctaat gtaatcatgt cgcctttttc aataatttta
     1141 tcacttgcaa caccatgtgg taatgcacct ctatgaccag atgctacaat cgtatcgaat
     1201 gatggtccat ctgctcctaa ttctagcatt ttgctttcta atattgcctt taattctttt
     1261 tcagtcatgc ctgcttttac aacagttaaa atatattcat atgtttcatc aacaatatta
     1321 gctgcttttt gaattaaagc aatttcgtca gcatctttga cgtctctaat tttatctaca
     1381 gtattagaaa tgcttattaa tgatatacgg cttttattta attcaaggta tgtatcataa
     1441 cttacatgat gcccctcaaa acctacattt tcaaaatttt cttggtgtag caattcttta
     1501 atctcaccaa taatagtaga tttacgatta ataatttcat aatttggcgc ctgcttagtt
     1561 gcttgatcaa tatatctaaa gtctgttatc aaatattgtt tatctttaga tatgataagt
     1621 gctccactgg taccagtaaa acctgataaa tatcttctat tgtaatccga aagaatgata
     1681 atcgcatcta aatgtttttg ttctaaaata cgatgcactt gtgttattct gctcatctta
     1741 ttacctccta gtgaaacgtt tgtgcatttc aactttatac attaaaataa tatcataata
     1801 aggataaaaa ataatagata ttgattttag ggagatagta atgaaaaaat tggtttcaat
     1861 tgttggcgca acattattgt tagctggatg tggatcacaa aatttagcac cattagaaga
     1921 aaaaacaaca gatttaagag aagataatca tcaactcaaa ctagatattc aagaacttaa
     1981 tcaacaaatt agtgattcta aatctaaaat taaagggctt gaaaaggata aagaaaacag
     2041 taaaaaaact gcatctaata atacgaaaat taaattgatg aatgttacat caacatacta
     2101 cgacaaagtt gctaaagctt tgaaatccta taacgatatt gagaaagatg taagtaaaaa
     2161 caaaggcgat aagaatgttc aatcgaaatt aaatcaaatt tctaatgata ttcaaagtgc
     2221 tcacacttca tacaaagatg ctatcgatgg tttatcactt agtgatgatg ataaaaaaac
     2281 gtctaaaaat atcgataaat taaactctga tttgaatcat gcatttgatg atattaaaaa
     2341 tggctatcaa aataaagata aaaaacaact tacaaaagga caacaagcgt tgtcaaaatt
     2401 aaacttaaat gcaaaatcat gataggagtc ttttaatgcg taatataata ttttatcttg
     2461 tacttattat tgctgcgatt ggattagtaa tgaatctaga tgcctttatt ttttcaatcg
     2521 tcagaatgtt aatcagcttt gctgtaatag ctggtattat ttatctgatt tattatttct
     2581 tcatcttaac tgaagaccaa cgcaaatatc gcaaagcaat gcgtaagtat aaaagaaatc
     2641 aaagaagaaa atagataaaa aaacggaagc acttgtaggt aaaatagtct acgtgcttcc
     2701 attttttatt ctaaaaacta ctttctaaac atccattcat ctgaacgata tttttcagtt
     2761 aattcttcca cttctgccaa ttgagcttct gttaattcaa gtggcttaaa ttctatattt
     2821 aaacctttct taaaaccttt ctcgaaagct tcttccattt gactaatagt aatgtgttca
     2881 tctgaaatat cattgatggc aactgctttt tcaacgaatg cctctttcat ttttaatttt
     2941 aatctttcat ttttataaat aaacatatca aacagttcat caatatcaat atcttgtaaa
     3001 atcgaaccgt gttggaggat tacgcccttt tgtctcgttt gagcactccc agcaatctta
     3061 cggccttcaa caactagctc ataccaactt ggtgcatcaa aacacactga acttcgaggt
     3121 tgttttaatt tttgacgctc ttcaggcgtt ttaggtaccg caaaataagt atcaaatcct
     3181 aagtttttaa atccttctaa taatccttgt gaaatcactc tgtacgcttc tgtaactgta
     3241 gaaggcatat tcggatgcga ttcaggcaca atcacactgt aagttaactc tttatcatgt
     3301 agcaccccac ggccaccagt ttgacgcctt acgagaccaa aacctttctc tttaacctta
     3361 tcaatatcaa tttctttttg tagcctttgg aaatacccta ttgataatgt tgcaggattc
     3421 catgtgtaaa aacgtataac tggatcaatt tcacctctag agacaaaatt taataacgct
     3481 tcatccattg ccatattata atatgggtct ttacttcctg tattaataaa attccaagtt
     3541 tcagtcatat tcaagaactc ctatcagcaa aatgtataat atttgattcg ctaattaatc
     3601 aatttaacta aatgaataat aattgcaatt ctttagtgaa atattttgat aatttgacct
     3661 aacagtctta taattatatt atcgtttaat tagggaggat gcaagatgag tgctagtttg
     3721 tacatcgcaa taattttagt tatagcaatt attgcttata tgattgttca acaaattctt
     3781 aacaagcgag ctgttaaaga attagatcaa aatgaattcc ataatgggat tagaaaagct
     3841 caagtcatcg atgttagaga gaaagttgac tatgactacg gtcacattaa tgggtctcgc
     3901 aatattccta tgacaatgtt caggcaacga ttccaaggat taagaaaaga tcaaccggta
     3961 tacttatgtg atgccaatgg gattgctagc tatagagccg ctcgtatttt gaaaaagaat
     4021 ggatatacag atatctatat gttaaaaggc ggctataaaa aatggactgg aaaaataaag
     4081 tctaaaaaat agtttttgta aatttaatat acgatttaat aaaatctgag tgttaattga
     4141 tcatcaataa caatactcag attttaattt tttaacaaag tctgttacta tatttctcta
     4201 gcttcactga tcattaaact tagtttcagc ataataaaga aagttcagct cattttcaat
     4261 acgattcaat taccgcaatc taaaaaatga aaagacaatt tctatgaaag aataatacca
     4321 aaccctaaga gttattactt cggtttagtt ttcttgttta aatagaaatt gtctttttca
     4381 attgattttg aaaccattat ccttaaatct tcatacaaag ttagaataat aattctcgga
     4441 atatgtgttt aatactttat ttttcctgtt taagattttc aaactttaat attggtttac
     4501 gagcagctgt agcttcgtct aatcgatcaa tcacagttgt atgtggtgct tctagcactt
     4561 tatcaggatc atttttagct tcttcagcaa tactaattaa tgtatcgata aaataatcaa
     4621 gtgtttcttt agactctgtc tcagtcggtt caatcatcat accttcttca acatttaatg
     4681 ggaagtatat tgttggtgga tgtacaccga aatctaataa tcgcttagcc atgtctaaag
     4741 tacgtacacc aaattctttt tgacgcacac cacttaacac aaactcgtgt ttacaatatt
     4801 gtttataagg tatttcaaag tgtttagata aacgtgcttt aatataattc gcattaagaa
     4861 ccgctgcttc agaaacctct ttaagtccag ttgctcccat agttcgaata tacgtataag
     4921 ctcttaagta aataccaaag ttaccataaa atggttttac acgtccgata gaatttttaa
     4981 tgtcattatc atatttaaat ttgtcgccat ctttaataac cattggcttt ggtaagtaac
     5041 ttgctagttc ttttactaca ccgactggac ctgaaccagg accgccacca ccatgtggac
     5101 cagtaaatgt tttatgcaag tttaaatgaa cagcatcaaa tcccatatct cctgggcgaa
     5161 ctttgtccat aatagcgttt aaattcgcac catcataata taatagacca ccagcattat
     5221 ggacgatttc acggatttcc ataatatttt tttcgaaaat acctaaagtg tttggattag
     5281 ttaacataat agctgctgta ttttcattta caacacgttt caagtcatca atatcaactt
     5341 cgccacgttc gtttgatttt acagtaactg atttaaatcc tgcaaatgaa gctgaggctg
     5401 gattcgtacc atgcgcagaa tctggcacaa tgacttcatc acgatgacct tcaccattat
     5461 tctcatggta agctttaaat atcatcaatg cagtccattc accatgtgcg ccagcagctg
     5521 gttgtaatgt cacctcatcc ataccagtaa tttcttttaa ttcttcttgc aaactataaa
     5581 taatttctaa tgaaccttga acttgatctt catcttgtaa tggatgtgat tcactaaatc
     5641 ctggtattct agcaaccttt tcattaattt tagggttata cttcatcgta catgaaccca
     5701 atggataaaa tccgttgtct acaccgaaat ttttatttga aagttcagta taatgacgta
     5761 ctaagtctag ttcagcaact tcaggaaact ccgctttgtt tttacgaata aatttatcat
     5821 ctaacaatga ctcaacagaa tttgttttaa tatcactttt tggtaatgaa tatgcatatc
     5881 tgccttcacg agatctttca aaaattaatg gacttgattt actagtcatt taactcacca
     5941 gccttttcta caaatgtatc gatttcatct tttgttctta attcagttac agctattaac
     6001 atgtgatttt taaagtcgtc tgaaacaaca cctaaatcaa aaccaccgat aatattgtac
     6061 ttcactaatt cctcgttaac ttgttgaatt ggtttgtcaa atttgactac aaactcattg
     6121 aaagatgtac catctaatac ttcaaaacct tttttaataa attgttgttt agcatagtta
     6181 gcatgttcta tattttgaac tgcaatatca tagatacctt gtttaccaag tgctgacatt
     6241 gcaattgatg acgctaaagc atttaatgct tggttagaac aaatattaga tgtcgcttta
     6301 tcgcgtcgaa tatgttgttc acgtgcttgt aatgttaata caaagccacg attaccttca
     6361 tcatcttgtg tttgaccgac taatctacct ggcactttac gcattaactt tttcgtcgtt
     6421 gcaaaatatc cacaatgtgg cccaccgaat tgagcaggaa ttccgaatgg ctgagtatca
     6481 cctacaacaa tatctgcacc aaatgaacct ggaggtgtaa gtaatcccaa tgctaatgga
     6541 tttgcatata cgataaataa tgctttttta tcttcaataa agctatgaat cttttcaaga
     6601 tcttcaattg aaccgtaaaa gtttggatat tgtactgcaa cagctgctgt ttcatcatcc
     6661 actgctgctt ctaatttttt caaatctgta acagtgccat ctaaatcgat ttccactact
     6721 tcgaattcct tacgcgtctt agcataagta tgaagtactt gtaatgcttg ataatgtaaa
     6781 ccttttgaga ctacaatttt atttttcttt gtttgactaa atgctaagat acatgcttca
     6841 gcaaagctag tcatcccatc atacatagaa gaatttgcta catccatatc tgttaattca
     6901 caaattaaag tttggaactc aaaaatggct tgtaattcac cttgagaaat ttccggttga
     6961 tatggcgtat atgctgtgta aaattctgat cttgaaatca tagcatccac aactgatggc
     7021 gcgtaatgat cataaacacc agcacccaaa aatgatgtat gcgtttcttt agtgatattc
     7081 ttgcttgcaa tgcgatttaa tcttctaagt aacgttgttt ccgcttcgcc ttcagcaata
     7141 tttaaatctc tatttaataa aatgtcactt ggtacatcac cgaataattc tcctatagat
     7201 tttgcaccaa ttgtttgtaa catttcttgc ttgtcttttt cagttaaagg tatataacga
     7261 tgactcacaa tgcacacccc tttttaatta tttatcaatt tgatttttct taacaatttt
     7321 cgcttttaat tgacgcttac gaacttgaac aagcaactct ctacccattt caaactcatc
     7381 tctttttatc attgcaagtg caattgattt tcctgatgat ggagactgtg ttcctgaagt
     7441 tacttctcca ataatatttc catctaaatc cataacttca taaccagttc ttgcaattcc
     7501 tttttcaagt aattctaatc ccactgttct tcttggtgca ccattttctt tttgatcttt
     7561 taatacagat ttaccaataa aatcagcatc aattaatggt ttacttgcaa aagcgatacc
     7621 accttcatat ggtgtaattg attcagttaa atcttgtcca tgtaatggca atccagcctc
     7681 taatcttaat gtatcacgag cgcctaaccc acatggcata acattatact ctaataaacc
     7741 atcccaaatt ttttcagtat catcgatatt acaataaatt tcaaacccat cttcacctgt
     7801 gtaacctgac tgagataaaa tgacgtttgc tccaaataat ttgacaccct gtttaaattc
     7861 aaacattttc atttcagtta catcttcatc aactaattga ttaattaaat ctctagcttt
     7921 tggtccttgt attgctaatt gaccatattg gtttgataca ttttgtactt caacatcaaa
     7981 tttctctttg tgttttaaaa tccaattaaa atctttttca gtattagcag cattaacaac
     8041 taataaataa ttgtcgtcag ctaatttata tattactaaa tcatcaataa taccgccttc
     8101 ttcattacat aaagcagtat ataatgcttt tgaagtagtt aaattatcag tatcatttga
     8161 taataaatat tgcacaaact gactagcatc tttacctgtt acttcaattt cacccatatg
     8221 actaacatca aacaggccaa tttcgtatcg aacagcatta tgctcctctt taatacttga
     8281 aaattgaaca ggcatcgccc atcctccgaa ttccacaatt tttgcacctc tatcaacata
     8341 attttgatat aaaggtgttt gttttaaatc acttgacatt gaaatacccc cttaaaatta
     8401 taaaaacagg atagcaatcg ctaccctgtt tataaatcac caattcagga acgcgttaca
     8461 gtattatcct tttgcct
//
LOCUS       DANJFZ010000112         8431 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.112, whole genome shotgun sequence.
ACCESSION   DANJFZ010000112 DANJFZ010000000
VERSION     DANJFZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.112"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            79..1602
                     /locus_tag="PWN45_002244"
     CDS             79..1602
                     /locus_tag="PWN45_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623989.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1670..2836
                     /locus_tag="PWN45_002245"
     CDS             1670..2836
                     /locus_tag="PWN45_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623990.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2833..3753
                     /locus_tag="PWN45_002246"
     CDS             2833..3753
                     /locus_tag="PWN45_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0623991.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3767..4426
                     /locus_tag="PWN45_002247"
     CDS             3767..4426
                     /locus_tag="PWN45_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0623992.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4478..5428)
                     /gene="lytM"
                     /locus_tag="PWN45_002248"
     CDS             complement(4478..5428)
                     /gene="lytM"
                     /locus_tag="PWN45_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDL0623993.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5754..6746)
                     /locus_tag="PWN45_002249"
     CDS             complement(5754..6746)
                     /locus_tag="PWN45_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL0623994.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6980..8356
                     /locus_tag="PWN45_002250"
     CDS             6980..8356
                     /locus_tag="PWN45_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0623995.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2835 a         1433 c         1358 g         2805 t
ORIGIN      
        1 acaaaattta aaataaacca tttaataaaa tgtattgttc tgatatgatt aactcataaa
       61 taattgggag gaagcaaaat gtctaaaaag ttaaaaatta taattcctat tattattgtc
      121 ttactattaa taggtggaat cgcatgggga gtttatgcat tttttgctaa tacaccgaaa
      181 aatacatacc taaaaagtga acaacaaaca gcaaaaatgt ataaagatta ttttaatgac
      241 cgttttgaaa atgaagtgaa gttccaagaa aagatgaaag ataattcgtt tttatcttca
      301 ttagaattaa gcgcagatgc atcagatgaa attgttaaag ggcttggcat tcctaaatct
      361 gttgttaatg catcgaaaat taaaatgtca tatggacatg atcctaaaaa agagaaatca
      421 atgattaatc ttgaaccaac aatcgcagac tctgcattag ggaaattcca gttagctgca
      481 gataaagata agcattattt cgaatcacca ttatttaaag ggaaatatag tgttaataat
      541 tctgatttat tatcaactta ttcaaaactt acaggtgaag atgaagaaac agcaaaagaa
      601 aatggtatta caaaccaaca actaaattta aatactcttt ttaataatgc tcaagcacaa
      661 caaagtgact acagcaaaat tgccgaaaaa tattccgaac ttattgtcga caaattagat
      721 gacgataatt ttgataaagg taaaaaagaa gaaattaagg ttaatggtga aaagtacaaa
      781 gttagacctg tcacgttaac acttagcaga gctgacacta aaaaaattac attagctgta
      841 ttagaagaag ctaaaaagga taaagacctt aaaaaattaa tggaagaaca aggtgctaca
      901 aaagactttg aaaaagacat caaaaaagca attgacgatg tcaaagaaac taaaaaggat
      961 gaatttgcta aaattcaatc taaaatttat accgaaaaac atacgattgt aaaacgagaa
     1021 attactatta cagacaaaga aaataataaa actaaaatca aaggtactaa tactttagaa
     1081 gacgataagt taaaactaga ttacgcactt gatttcgatc aagataaata cacgtatgct
     1141 gaagcgaaat atacaattaa aggcgtatct tctaaagaaa aagataataa atataatgat
     1201 aaatacgaat ttggtaaaaa gacagaatat gatgaatcaa aaatcaaatt agataaccaa
     1261 gaaaaagtag atggcacaaa acgtcaagat aaaggtaaaa tcactgtcgc attagataaa
     1321 tatagcgacg aaaatgaatt cacttttgaa aataatatag attctgacgt aaaaaataac
     1381 actcagaaat ctacgttaaa tatcggcatc aaatatgctg aagaaccaat taatttcatt
     1441 ttaaaatcta gcacaaaatt gaaagcagat attgattttg atgatagtgg tgcaaaagat
     1501 ttcaatagtc tatcttcaaa agaccgtgaa aaacttgaaa aagaaatcga aaaaaatggc
     1561 ggcaaaatgt ttgaatcaat tttaaaaaag gcatctaaat aactaaaata taaaaataag
     1621 attggaaggt catcttgcct ttcaatctta tcattggagg agtggttaaa tgaaaacaat
     1681 acatttgttt cgcatctacc actctttttt attgaaaaag tggtatttga ttatctattt
     1741 actatttata ttagcggcac tccttattac attaacgaca atccaacatg taacagaaga
     1801 tgacaatcat tttaatatag gtgtcgtaga taaagatcaa tcaagtgaaa cgaaattaat
     1861 cttaaactct attggtaaag ggagtaacct aggaaaaaac gtgagcatta aagcatatga
     1921 tgataagcaa gcacatactt tgttaaaaaa acataaactt caaggctatt ttgtttttga
     1981 taaaggtatg accaaggcat tttataaaca aggcgaacta ccaatttcag tatatacata
     2041 tgatcaacaa tccatgaaaa gtgtcgtgct atctcagcta acagattctg tttaccaacg
     2101 tcttatgcga tcaatgggtg gcatcttagc ttttcaagac ttagcaccga aagcatcaca
     2161 ttctgacagt atcaatgtta tgactgattt gctgattaca ggattaaacc gttcaggtgc
     2221 atttaactta gaaccgattc atttatacga tacgggcagt tattatgcaa ttacaggatt
     2281 tttaacaacg gtattcatct ttgcactatc tttatttaca gttttgaaaa tgaatcaaga
     2341 tactgtattg aaagcgcgat tgaaaatgtt tcatttttct aaagagcgtt tattaatcat
     2401 tcgtacgttg attacatggt tttatactat gttatggagt atcgttggtg tagtttggat
     2461 tgtgttcagt attccgaata tctttgaatt atataattgg ccaacgttag ccattcattt
     2521 aagctattat gttacctttt taatactgtg gttattactc atcgaacttt taacaacagg
     2581 cttgttgaat agtattagta aagtcatatt agctattgtc atcctggtgt tatctggttt
     2641 aacaatacct acgatttttt tacaacatat agcaaatggt gttttcaata ttcaaccgtt
     2701 cgcagttgta acaaatcaat tgttagaaat tattttaaac aactacattt tagaactgca
     2761 ccctagtttc tatcttagct tcatcgcact tttgataatt aatttagctg tattggtttg
     2821 gaggtatcgt caatgaagcc ttatatacaa cttgttgtgt tcaagcaatg gttacaatac
     2881 atcttgctcg taacaaccat tgtcatcgca ctcgtactta ttggtatcgg ttaccgtgta
     2941 gcacatgaca acttcaaaat accgattacc attcaagatt tagaccaaac cactgcatca
     3001 aaatcattcg tcaataaaat taaacaatct gactatgtaa ctattaaaaa agtcgatgaa
     3061 gatgaaagct atattgaaga tgatgttact aaaaaggaag ctattttaag tatgcaaatt
     3121 cctaaaggtt tctctcaaaa attaaaagag aaccgtttaa aagaaacgat acagttatat
     3181 ggtagagatg actttatagg tggtattgct gtagaaattg ttagtagttc attatatgag
     3241 cagcaaattc ctaacattat ttatgaacac cttgaggata tgaaacagca tcaatccatc
     3301 gatgctatca acaagtccta tcataaacat acacctgaat ctaaaatcaa atttgtgtcg
     3361 cttactaaac aagcacaaca ctctatttca attagcttaa tctttgcggt gattttattt
     3421 gttagcgctg ttcaagtagt acttcattat cgtttaaacc aacaagcagc attgcaacga
     3481 ttatcacaat atcatttaag ccgtttcaaa ctatatagta cttatgtaat gacacatacg
     3541 attttgttat tgttggtact attggcagtt agtctatatt tgtctcaacc actcagctta
     3601 atattttact taaaatcact gttacttata ttgatttatg agataggtat cgtctttatc
     3661 ttattccata ttcaaacaat aagtcatcga ttattcatga catttatata tgcacttgct
     3721 atgggtatcg tatacttgat tattttcatg taaaggagcg taactgatga tagaaattaa
     3781 taacctttca aagcgttacc gtaacaaaca gattttcaat catttaacta tgtcctttga
     3841 tagtaatcgt ttaaccgtat tacttggtga taatggtgct ggaaaatcaa cattacttcg
     3901 tatgattgct ggtattgaaa aagctaatga tggaactatc aactatttcg gcgaaaaatg
     3961 gaatcaaaga caaatacaaa atcacatcgg ttatgtgcca caagacattg cgttatttga
     4021 acacatgaca gtggctgaaa acattaaatt ttttaaatca ctttgtaaaa atccaattaa
     4081 cgatacaact atcaacgaat atttacagca attaaacttt gatgatacgt ctgccaaagt
     4141 atctacattg tccggtggga ataaacgtaa aattaatata ttagtaggtt tactaggtca
     4201 acctcgaatt ctcattttag atgaaccgac agttggtatt gatttaaaat ctagacatga
     4261 catccaccaa ctacttaaca tcatgaaatc taaatgttta attatattaa ctacccatca
     4321 tttagatgaa gttgaagcac ttgcagatga tatcaagtta attggccaag atccttttta
     4381 tcaacatgtt ttagaggaca aacaatggac ttatacctat tattaaaacg aaaaaatccc
     4441 aagctgcgta tgatatcgca acttgggatt ttctgtatta tctactttgc aagtatgacg
     4501 ttgggtctac tgcatattga ttaccgatgc caccagacat acgttggaag tgtacgtgag
     4561 gcgctgttga attacccgta ctacctgaat atgcaatttg gtcaccagct ttgactttat
     4621 caccagctga aacagttaaa cgattattat gcatatacca ttggtagtta ttactgttcg
     4681 cttctttaat cgttacttga ttgccgccac catagttact ccaacctgct tgtactactg
     4741 taccatcagt taatgagtaa actggtgaat tttcaggcat tgcatagtcg acaccgtaat
     4801 gcgcaccacc accgtgatat tgtccatatg gttgtagttg tttacgactt gttaaccagc
     4861 ttgcgtcttt cgcatgacca ctagctgtcg ctttacttgc tgatccacca ttttgattag
     4921 atgtgccatt aggataattg acctttccat taccatcatg gctgttatac gcttggttgt
     4981 tgttactatg tgaataatag ctcgcgtctg gacctacatt tgattgataa ccatattgat
     5041 taatatgctg ttggctttga ctcgctgtgt agtcattgtt atctcctgct gttgctggat
     5101 tcacatatgt ttggccgctt ccattggcat ttgcattttt tggataacag ttataaaaat
     5161 aatgcgtatg tccttgagca tctacgaatg tatagctata ttctttatta tcaaacattg
     5221 cttgattcca gttaccatca ggtgtgtgat gataatcccc attagaatca attgtataat
     5281 aagtaccata agatacgtct tgtgattgtg ttgacatttg tgtatgtgct tgttgggtgt
     5341 ttgtcgtttc tgctgcatct gcttgatgcg ccattgtaaa tgtagcgaag cccatcgttg
     5401 caatcgctgc tgctgttaat tttttcatgt ataaaacatc ctccattaaa gttaaagtta
     5461 gttttcaatt aaactgtact gcacatacta aaagaattag acaactgagt aaaggattta
     5521 attctcattt tccaactatt taatattccc gaaatgtttt actaaactca ttacattgtc
     5581 attacaaaat agccatacat tgatattaaa atgacatctc tcactgcatt cgtttaacct
     5641 tttataaatt ttcaaaatta acaactaatc gttcgtccat gttcgcgatt caacgctaat
     5701 gcatgatagt aatcatccat caaatcatat caaccaaatt ccattatcaa tcgctattga
     5761 ttgtcattca actttctaat agtgatatgc ttctcaggct taaaaatcgt catatctttt
     5821 ctattaatta aatcatctgt gagctttaat gctactaatt cattgctgcc ataatactta
     5881 atatataacg ttcttgtagt taaatttatt acggtctgat acatcgtata gtgattgtca
     5941 gcatcatgcg gacgtacaat tccaatcggt atatttaccg catctaataa ataaaatgca
     6001 ttcattaaat ccatttcttt atcattgttt tgagcaatgt ttgctttcat aaatgccatt
     6061 ctcacaaagc gctcagttga agtaaatcca cctggcaatc caaatgtacc tgcttcattg
     6121 cctaaaggtt caatcgttac accttccaat aaatttgctg ttgctggata aggagaaata
     6181 ttgatatatt gtcttaaatt actataatgc caatttaagt ctggatgatt tgttaagaca
     6241 ccaataggat tatcttttat aaccacttca ccctctttaa atgaaacttc gactgtatgt
     6301 ccagttgcat cggaaacatg ataatgcaat ggcggaactt caccgatgtc atttaaatat
     6361 acagctacaa catgtatttg ggatgcttgt tgtttcatat cttcaatgct tgttgtatat
     6421 cccaaaatcc atgtcacaat ttcattttgc gtaatattca tcgcgtccgc tttgtgtgtt
     6481 gatccatatg aactataacc tcggaaatat tgtgttgaaa tggcaacgcc atgttcatta
     6541 acaccatcac cataaataaa accttccata tctgttcctg tgccaataaa gccatattgc
     6601 gtttggcctg tcgtgccagt gcgagatttc caacgataat ttctaggcgt cactgctggc
     6661 gaaccatcta atggataatc ataatccatc gtgcgtccaa gaagtacttg attatttaaa
     6721 gtttgtattg tgaatcctgt gcacattgtt ctcactcctc tgtaccttca tttactttaa
     6781 tcactttcaa ataaagctgt ttcacttaaa catactataa aaaatcaatt atacaagcaa
     6841 ttaattgata ttcattctca ataactgtgg tatgatatgt aaggaaatca tgacttatgt
     6901 gtgagtgaac gatcatctat acatccgttc acttcatctc atgactttct atatttaatt
     6961 tttacaagga gtgacatctg tgaataacac acaatcttca ccacgcagta atattattat
     7021 tgcgattatg ttgtctgcat taacatattg gttgtttgca caatcattta ttaatatagg
     7081 acctctcgtt ggtcaaacgt atcaaacctc tcctgccgtg ttaaatttat ctattagttt
     7141 aacttccttc gccacaggta tcttcatggt ggctgcaggt gatattgctg ataaaatagg
     7201 acaactgaga atgacataca tgggtctcat aatcagtatg tttgcatctc ttctattaat
     7261 tatatcggac atcactgcac tgctcatcat cggtagaatt ttacaaggtc tatcagcagc
     7321 tatcttgtta ccttcaacag ttggcgtgtt aaataatcaa tttaaaggag aacatttaag
     7381 acgagcgatt agttatctaa tgattagtac tgttggtggc atcggcctag ctggtgttat
     7441 cggcggttta attgcctcaa atttcggatg gcaaacgaat ttcatcatta gtatagtcat
     7501 tgctttcatt gccatattgc ttctaaaagg cacacctgaa aaagtaagtc aacatagcca
     7561 ccgtcatcca ttcgattaca aaggtatgtc gattttcgct gttatgattg gtagctttac
     7621 attattgtta acacaaggat tcgaacaagg ttggtttagt acattttcaa tcatttgtct
     7681 gagcattttt atcatcacta cgttgatatt catcatcatc gaacgtcgac atgaagtacc
     7741 ttttattgat ttctcagtat tacgcaaccg tccgttcatt ggtgcatttt taaataactt
     7801 tgttttaaat agcggtctag gcgtaacagt ggtctttttc atatatgctc aaacacacct
     7861 tggtttatca gctgcgcaat ctggacttgt tacattgcca tatgccattg tggcagttgc
     7921 gatgattcgt ttaggtgaaa aagcaacatt acgtttcggt ggcaaattga tgctcatcat
     7981 tggtccgttg ttccctgtca tcggcatcac tattattagc atgactcaat tattggcatc
     8041 acaatatgtc attgcagtta tcattggttt cgtcatatgt gcgataggta atggtttagt
     8101 cgcaacacct ggacttacga ttgcaatttt cagtatgcct aatgaaaaag ttggtttagc
     8161 tacaggatta tataaaatga gtggtacatt aggtggcgcc tttggtatag cactaagtac
     8221 tacagttttc agtatgttac aactaaacta tgcaccaagt gtagctgcaa ccgtaacatt
     8281 tatagtcagc attgtattga tgatccttgg ctcattgtct gcatacatga tcattccaaa
     8341 aacagttaaa tcttaaatat aatagaagaa ttatgtttcg aaatatcttt atcactttaa
     8401 aatgatatac aagaaatcca agaaaaataa g
//
LOCUS       DANJFZ010000113         8328 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.113, whole genome shotgun sequence.
ACCESSION   DANJFZ010000113 DANJFZ010000000
VERSION     DANJFZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.113"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(74..1132)
                     /locus_tag="PWN45_002251"
     CDS             complement(74..1132)
                     /locus_tag="PWN45_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDL0623996.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(1119..1325)
                     /locus_tag="PWN45_002252"
     CDS             complement(1119..1325)
                     /locus_tag="PWN45_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0623997.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(1550..1894)
                     /locus_tag="PWN45_002253"
     CDS             complement(1550..1894)
                     /locus_tag="PWN45_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDL0623998.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(2565..4937)
                     /locus_tag="PWN45_002254"
     CDS             complement(2565..4937)
                     /locus_tag="PWN45_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0623999.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(5029..5277)
                     /locus_tag="PWN45_002255"
     CDS             complement(5029..5277)
                     /locus_tag="PWN45_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDL0624000.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(5278..5661)
                     /locus_tag="PWN45_002256"
     CDS             complement(5278..5661)
                     /locus_tag="PWN45_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDL0624001.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(5663..5866)
                     /locus_tag="PWN45_002257"
     CDS             complement(5663..5866)
                     /locus_tag="PWN45_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDL0624002.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(5833..6006)
                     /locus_tag="PWN45_002258"
     CDS             complement(5833..6006)
                     /locus_tag="PWN45_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDL0624003.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(6018..6290)
                     /locus_tag="PWN45_002259"
     CDS             complement(6018..6290)
                     /locus_tag="PWN45_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL0624004.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(6283..6546)
                     /locus_tag="PWN45_002260"
     CDS             complement(6283..6546)
                     /locus_tag="PWN45_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624005.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            6739..7071
                     /locus_tag="PWN45_002261"
     CDS             6739..7071
                     /locus_tag="PWN45_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624006.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            7083..7541
                     /locus_tag="PWN45_002262"
     CDS             7083..7541
                     /locus_tag="PWN45_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0624007.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            7558..8286
                     /gene="seq"
                     /locus_tag="PWN45_002263"
     CDS             7558..8286
                     /gene="seq"
                     /locus_tag="PWN45_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HDL0624008.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
BASE COUNT         2632 a         1460 c         1094 g         3142 t
ORIGIN      
        1 ccgccggtac ccggcgaaaa aacattttaa gccgatgggc agggggctat aaaattttat
       61 ttaaataatt tttttattta atattaacta tgcgcaataa ctctggaatt atttttatat
      121 caaacacata gttcaatgca aattgataaa taccttttaa tgcttgtttc tttttgttat
      181 acataatagg ttcgtattta gcatatatgg catgtccaaa gtctccaaca aagacatcat
      241 gtgcatcatc agtaaccact acattagtca cattaaatag gttacttaaa tcacctgcaa
      301 gtccacctaa tcccatctgt gccaactctt tgatgagctt atcatgatgt tctgtgttca
      361 tcacaataga tacattacgc ctaaacgttt gtggtatatc tttcatcgct tgcttgatac
      421 catcgtatat tgatgtgtta cttacttctt tgataccttg attataaagt gataagtctt
      481 ggttaccttt atcaattaaa ccacttgaat tattcttatc taataacaat gttgtttcat
      541 atgcgacaca ctcattaata aatatctctt taagtagatc atctaatgtt ctatcagagc
      601 catgaactac actgtctgat acttctgtgt agatatgtcc ctcacgtcta tcaaagtatt
      661 tactactgcc attaagtgat agctcaatac cgttagtagc taacttatct ttaatgtatg
      721 ttagtgatgg tatagattca acatgttcat catcagtaat ggtaatcacc tcacgtagtg
      781 attgaggtag ctcacaattc accaataact ctttaaatac ttctttacgt gcagttggta
      841 actcttgaat attaaagtta ttctcactac taactaattg ttttgttaag gtacctaccc
      901 atgtattact tgattgattt acttcattca tatttaacac tcctaataat tttttgttaa
      961 tttgctctgg taataaaact tctgtttcag taatacttaa ttgctttaac ccctgttcaa
     1021 tcatacttac atctttacct atatcctttt gatatagatt ggcatatcct ttaatatcca
     1081 tatcatcaaa gttagatagt tgatacccaa attgttcttt aaactctttc ataatcttca
     1141 tctcctaatt tgtttttagt atgaacaact aaaccatata tacgttcgtt gtttttatct
     1201 tcttcataga acgaaatgtt atgaagaaag tatttgtcta ataattcgtg atacttattt
     1261 aatagttctt gcttatctct atacttaaac acacgtttta cagattcatt tgtatgatct
     1321 agcacgtctt gcctctctcc ttttaatctg atagatatac attttaataa gttcaaaatc
     1381 agcacgcttt tttgtatagc gccttacaac gcgatttata tatttcgtat tcattaattg
     1441 aacgttcaca ggtgtatgtt tgattatttg atatgcgatt cttttatgat taataaatat
     1501 acacactccc atatctatac ttgatatttt aaatgcttat ctgcatgttt taatttacta
     1561 aactaaatgt tactggtagc caatgatcta tttttatatt tttattcttt acaattggta
     1621 gccttagacc tttaccatca ataagatagt acattggcgg acaaatttgc atttcaatta
     1681 gcccgtcgcg ttttaaatct gccacaatgt taaacgcttc ttcattccaa cccatccaaa
     1741 atataatatt cttatggttg ccactggtat atgcgccgtt acctttgtat ttaaaacctt
     1801 gttcttcaaa tacattctca atctctacaa atgaagtgct gtcattgttc tttatatatt
     1861 ctaagatgat ttgtttcatt ctatttctat tcatgttact ctagtcctcc aatctatcca
     1921 gttactaaag caaccacgtt agatactaaa aaccactgaa tttatgtttt tcaataacca
     1981 ctgtaaacac agtgatgtca atacatacag gctgttggtt actgagttac tgaaaattca
     2041 cggtatttta tataatcttt tattacctta attctctatt atacatttct actaaaaaga
     2101 aataatttat agtaactcag taactttttg tcaaaaagac taatatgaaa gtaaaaactg
     2161 tggttactga attctagaat tttagtatct tttagtgact taaacgtttt ttagtaactt
     2221 tagtatctta ggcaattttg tataactgca agtctcttcc gattatgtgc aactgaaatt
     2281 tacagctatg caactcgttt taaattttcc agttgcaatt tttttcttac tgtcacaagg
     2341 ggttaatcca cttttgcaac tatgcaaccg aaatcacaca aattagttta tataattttt
     2401 gatactattt cttatttata ttttggttat ttacttttct actaataagt tatagatttt
     2461 gagttgctca gttgcataag tgccctaaac ccttgatata actgtgatta taggtgcaac
     2521 ccgttttaaa attttcggtt gcattcggtt gcattcggtt gcaactatat tgcttttagc
     2581 ttcacctttt catacgattt atagtttttt ccactttcaa taggttcaat gtaatttcta
     2641 ttgtcattaa agttgtatat cctcttagca agttcttcat tgtcatatct tcgctgtgca
     2701 tcagttttcc aataattctc taaataatgt tcaaattgtt tataaaactt gtttgaactc
     2761 aatgcattat agccattttc atcacaatat tctttataaa atgcgtatac aatatatttc
     2821 ggcacttttc taatagtcca ttgatcaaac atatttactt taaacccata aactggatcg
     2881 ttatcttctt taaatacttc aagcattttt ttagatgcat caggaatgtc aaaggtttca
     2941 aaatctaaat taattgcttt ataaagcaca tactctagca cttgctgatt ttttatatag
     3001 tcttctttga ttttaaaatt ctctttaatg ccattaaaat tggcattaaa cggtactatc
     3061 aataaccttc ttaaagtccc gcctgttttg tctttaaatt taggcattcc attcgttgat
     3121 tgaataactg tacacttaaa agtcgctcta tataagggtt tatttttaaa ctcaaccaac
     3181 actggatcgc cagtaactac acttttaaag tttgaagaat catcgacata cacaccaact
     3241 ggtacatcgt ctccaattac tgctgtcttg ccctctaaca cactcaattt aaaacgttca
     3301 tcaaactcat tcacttttaa gctagcaata ttgctataac ctattacatt agacaataat
     3361 tcttgaaacg tccctttacc attatttcca tcaccaacaa agaaaatagc ctttttacga
     3421 gtgtagttac cattcattga gtcattaatt acttgccaca ataatttaac aacctgatta
     3481 tcattacaag caatctcttc tatccaccga tctatattcc agccatttat ttcaggtact
     3541 atatcttgcc ttacatacga tgtatctatt ttactggtaa atatataatc aggtgtaaat
     3601 gattctagtt gcttcgtttt acggttaaat acaccatttt taactggtat taagtatggt
     3661 gagttagttt tctcttttat atctaccata ttggttaaat gataaataac ttcatcagct
     3721 ttgttgctat tatgtttggg ctctaaatag gaaatcactc gttttataat tgtagtgttc
     3781 tgtgtatata tcccttcatc aaattgatac atagctaact tagtattttc ttcatcatca
     3841 aaaagtataa atgttaaatg ctcattcaaa atatatgcac attttatagg gctaattgtt
     3901 gtaggctttc gccctcgttt tccttcttca atccaatttt cttccatttt tgttctttct
     3961 tcatgcccca attgattcaa caactgttta acatctttac tcccctgtgg tataacatta
     4021 aattcatcta tcgatttagt ataaattgtc ttttgattct ttatagcttt atctaactca
     4081 agctgtcccc aagtcgtatt acctcgttta ctttcccatt tatccgtaag attattgtag
     4141 tttaaaaata tacgctccat ttgctgtttg tttttacctg tgtaaaacgc taagtagtgt
     4201 aataagcttt gtactgcttc gcttgaactc tcaaaatatg attcatatgt gccttgtaat
     4261 agatctttaa ttttatcttt ttgtttagat ttcatcataa tgtttataat atcttcatca
     4321 gataattgta tattggtatt agattcctct ctcacaacct catttgctgg caaattctct
     4381 ttaaaatatt catcaactag agtatttaat acttcttgat tatcacaaat atcactttga
     4441 ccaattgtgc atcctgtaac agtcataaac cttgctgaat catataactc tatgtctaaa
     4501 tctgttcttt tctttttacg gttatctggt agtttacctt taaagaaaca atgtaatcct
     4561 gtaccacttg gagacttttc acaatatgtg agttgcatca tttttagtgc taattcagaa
     4621 ttgatttgtc cttttttatc aattgcatta tcaatatcta ggcaaacaaa attattacca
     4681 tcacttaata caaaacctat accgctatat tgctcatcaa cttcatattc aatacttaca
     4741 ctttcaaagt cacaccatgt ttttttattg gttgtactag ctctgtaacc attaatacta
     4801 tatggtactt ttccaaaatt ttgttgcttc tcgttccatt cagctcgcca taatacccaa
     4861 ttgggcacta attttaattc ttgcggtatt tctaattctt taactccaat aattctatct
     4921 tttttcttaa ttgccacaga atgcctcctt tctaatctga tttctctgta ttttcaatgc
     4981 aactaatgtt aaaatattaa tggtatgatt ttataaattt ttattttttt atgcgttatc
     5041 ttggctttgg tcggcgaaag atgacgcttt ttctaatgca tcaaacattt gatcattgtg
     5101 aatacgtctc tcatgatatg aaaatccttc tttaatagca tcgtctttat ttaaggtatg
     5161 atttggctca tgtagatata aatcttcaaa atgcgagcca tgattatcct ttaaatcctc
     5221 aaacttttct ttcaatatct ttaaatcaca cattaaattt ttaatttccc aattcattta
     5281 taattcctcc acttcaatat ttcccacaat gtaatctaat gcccactcta acattccaat
     5341 cacgtgtcct ttgcgatctg ttgtatgttt atgttcacct ttttcgtcta tgacactata
     5401 acggtaaacg tgttgtgttt ctttcatgac atcacttagc gtcattgtta cttcatcaag
     5461 aattaaaaat gcttcatctt caaaatctaa ttcagcaaga atattgaaca atccattatg
     5521 aactaacttt aaagcatatt catataatgc tttatcctga taatgatagt ccttatcgga
     5581 ttgaaactta tcttcctcgt ataatactac ttctaatatt ttaaccactt ttgataattg
     5641 atatttttgt ttgatttcca tcttaactac ctactttttt cttattttta atttctgtaa
     5701 tttttgataa atccatttct aaacacgcaa cttgaacatc tttactaaca tcaggaaagt
     5761 actgttcaaa tacttttggc ggtatattta acattaaatt atgttgagta tctttaatgt
     5821 tgtaccagcc aactactgtt tttgtaataa taacttgttt tcgcacgttg taatctctcc
     5881 tgttaaatta aatccataag ttaccatcat gccgtacaca ctaaaagcga catacatgtt
     5941 agatattgct agtaataata ttgttaacaa tgaaactaag cagatataag ttaagtacat
     6001 tttcattgcc ttgcctccta catccatttt ttatgacgcg ccttcatgta ctcctcgaat
     6061 cgcggaatac tgataacaat cattgttgat gataacgagt aatataaatc atcaacacct
     6121 ttagaatctt tttcccactc ttttaaaatg cgattcaccg aactatatga aattcccaaa
     6181 atactagcta gtgcattagg tttcgcaaac aaaggattca ctacaacttg ctttggttct
     6241 gtaattgtat tttctttagc tggtacatca tgcaattttg ttctagacat ttaattcacc
     6301 tccttttaag atgttttttc gttcttttcc gggaacgtta ttggtaaaaa aaatgtctag
     6361 gttatttgtt tcataaccta atatttttgc cattttaata aattcattcg ctccaatatc
     6421 tactattcca ttttctctct ttgcataagg cgttcttgtt ttccacccca ttttgtgagc
     6481 catttcatct tgcgtaatac cacaagctat tctttccgct ctcaatcttt ttaaatttag
     6541 taccacgttt acacctccta tcgttctcgt tcgagaacta attacaattt accatttacg
     6601 ttctcgttcg tcaacaattt ttacttaaaa aaattcaaaa agttttttct tccttatata
     6661 ttgtattctt ttgggaacgg tgttataatc aaatcgttca caaataagaa caaatattca
     6721 attcaggaga tacaagaaat gagaactaat gatgaaataa tcacaataat taaaacatca
     6781 atgaaagaac aaaatatgtc actaagtgaa ttagctcgtc gtgtaggtgt agcaaaatca
     6841 gcagtatcaa gatatttaaa tttaactaga gagttcccat tgaatcgtgc tgaagatttt
     6901 gcgaaagtac ttggaataaa aacagaatat ttattaggat ttgctgaacg cgaagaatct
     6961 acaaaacaag atactatcgc cgcgcactta gatggagatt ttacagagga agaattaatt
     7021 gaaattagaa agtatgcgga gttagttaga aaagcacatc gaaatcagta aggggttatt
     7081 gcatgtattt atacgaaaag atggttattg aaaacaaaga gataccaatt gatgatggga
     7141 aatctttagg taattttgag ggtctctatg acaatggggt tattttaatt aataaaaatt
     7201 tgtcagaaac acgtaaagca gaagtgttat atgaggaact tgcccaccac aagttgacgt
     7261 atggcaacat tttagaccaa tcaaaatgga ttaatagaaa atttgaaaat tatgcacgta
     7321 gacacggctt tatctcagct gttccattac gtgaaattgt agaagcttat aattatggcg
     7381 tacgtaactt gtatgagttg tccgagtacc tacaactgag tgaaaaatac atattagaag
     7441 cgatagaaca atataaaaag atatacggta ttggtaccca ctacggcgag tattcaatta
     7501 catttgagcc gttgagagtt tttaaatata aagaaatata acaaaggaga aatgaaaatg
     7561 aataaaatat ttagagtact cactgttagc ttgtttttct tcacattttt aataaaaaac
     7621 aatctagcat atgctgatgt aggggtaatc aaccttagaa acttttatac taattatcaa
     7681 ccagaaacgc ttcaaggagt tagttctgga aatttttcta cttctcatca attagagtat
     7741 attgatggaa aatacacttt atattcacag tttcataatg aatatgaagc gaagagatta
     7801 aaagatcata aagtagatat ctttggaata agttactcag gtctttgtaa tacaaaatat
     7861 atgtatggcg gaattacgtt ggcgaatcaa aatttagata aacctagaaa tatacctatt
     7921 aatctctggg tcaatggtaa gcaaaacact atatctacag acgaggtttc tactcaaaaa
     7981 aaagaggtaa ctgctcaaga gattgatatt aagttacgaa aatacctaca aaacgaatac
     8041 aatatatatg gttttaataa aacaaaaaaa ggtcaggaat atggatatca gtcaaaattt
     8101 aattctggat ttaacaaagg aaaaattact ttccatttaa ataatgaaac ttcttttaca
     8161 tacgatttgt tttacaccgg aactggtcaa gcagagagtt ttttaaaaat ttacaatgat
     8221 aataaaacta tagatgcaga gaattttcat ttggatgtag agatttcata tgagaagact
     8281 gaataaatag aatttaagga gaaaatgcaa tgaaaaaatt aatagtta
//
LOCUS       DANJFZ010000114         8206 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.114, whole genome shotgun sequence.
ACCESSION   DANJFZ010000114 DANJFZ010000000
VERSION     DANJFZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.114"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            47..838
                     /gene="pgeF"
                     /locus_tag="PWN45_002264"
     CDS             47..838
                     /gene="pgeF"
                     /locus_tag="PWN45_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL0624009.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            862..1530
                     /locus_tag="PWN45_002265"
     CDS             862..1530
                     /locus_tag="PWN45_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL0624010.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1527..2090
                     /locus_tag="PWN45_002266"
     CDS             1527..2090
                     /locus_tag="PWN45_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL0624011.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2102..2392
                     /locus_tag="PWN45_002267"
     CDS             2102..2392
                     /locus_tag="PWN45_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL0624012.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2502..3281
                     /locus_tag="PWN45_002268"
     CDS             2502..3281
                     /locus_tag="PWN45_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL0624013.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3305..3922
                     /locus_tag="PWN45_002269"
     CDS             3305..3922
                     /locus_tag="PWN45_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL0624014.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4143..6896
                     /gene="ileS"
                     /locus_tag="PWN45_002270"
     CDS             4143..6896
                     /gene="ileS"
                     /locus_tag="PWN45_002270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL0624015.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7186..7983
                     /locus_tag="PWN45_002271"
     CDS             7186..7983
                     /locus_tag="PWN45_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0624016.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            complement(8044..>8206)
                     /locus_tag="PWN45_002272"
     CDS             complement(8044..>8206)
                     /locus_tag="PWN45_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0624017.1"
                     /translation="ELGFVLMAINIRKIAAQRAVHYKIHIKKADFYQIINRNQLFTLP
                     KNLMSQPPS"
BASE COUNT         2992 a         1156 c         1551 g         2507 t
ORIGIN      
        1 tacagttaga cgaagataat ttaaatgaaa tgatggtgac gatcgagtga atgataattt
       61 taaaaagcaa ccgcatcatt taatatatga agagttatta caacaaggta ttactctagg
      121 tattacaact agaggagatg gtttaagtga ctatcctaaa aatgctttta atatggcgag
      181 atatattgat gatcgcccat ataatattac tcaacatcaa ttgcaattag ctgaagaaat
      241 tgcgtttgat agaaaaaatt gggtgtttcc cattcaaaca catgaaaata aagtcgcttg
      301 tattacaaag gatgatatag gcacaaatat agacacttta actgatgcgc ttcatggtat
      361 tgatgcgatg tacacatatg atagtaatgt cttattaacg atgtgttatg cagactgtgt
      421 accagtatat ttttatagta caaaacatca ttttattgca ttggcgcatg caggttggcg
      481 tggtacctat actgaaattg taaaagaagt gctaaaacat gtgaactttg atttgaaaga
      541 cttacatgtc gttattggac catctacatc atcaagttat gaaattaatg atgatattaa
      601 aaataaattt gaaacattgc caattgatag tgccaactat attgaaacta gaggacgaga
      661 tcgtcatggt attgatttga aaaaagccaa tgctgcatta ttaatttatt atggtgttcc
      721 taaagaaaat atttatacga cagcgtatgc tacatctgaa catttagaat tatttttctc
      781 ttatcgatta gaaaaaggtc aaacaggacg catgttagca ttcattggtc aacagtaaac
      841 aaggaggaga tatgtttgcg tgtgaaagat aatttacaac aaatctcaac acaaattaat
      901 gacaaaagtg aaaaaaataa tttttcaaca aaaccaaacg tgattgcagt tacaaaatat
      961 gttacaatag agcgagctaa agaagcgtat gaggctggaa taagacattt tggtgagaat
     1021 agattggaag gcttttttca aaagaaagaa gcattaccat cagatgcggt gatccatttt
     1081 ataggatcat tacaatctcg aaaagttaag gacgttataa acgacgtaga ttatttccat
     1141 gctttagatc gattgagctt agccaaagaa attaacaaac gtgcagaaca taaaattaaa
     1201 tgtttcttgc aagtgaacgt ttcgggagaa gcttctaaac atggtattgc tttagaagat
     1261 gttgatcagt ttatagatga tcttaaaaaa tatgacaaaa tcgaaattgt aggtttaatg
     1321 acgatggcac cattgacaga tgatgaagca tatattagat cgttatttaa acagttacgt
     1381 ttgaaaaaag aagaaataca acgactcaat ttagaatatg cgccttgtga tgaattatca
     1441 atgggaatga gtaatgacta tcttattgca gttgaagaag gtgcgacgtt tgttagaatt
     1501 gggactaaac ttgtaggaga agaggagtga gccacttggc tttaaaagat ttatttagtg
     1561 gattttttgt aatagatgat gaagaggaag tagaagtacc tgacaaacaa caacaggtaa
     1621 atgaagcgcc agcaaaagag cagtcacaac aaacaacaaa acaaaacgca atcaaatcag
     1681 tccctcaaaa atctgcatca agatatacaa caacgtcaga agaaaggaat aaccgtatgt
     1741 ctaattattc aaaaaataat tcacgtaatg ttgtaactat gaacaatgct acaccaaaca
     1801 atgcatcaca agaaagttca aaaatgtgtt tattcgaacc acgtgttttt tcagatacac
     1861 aagatattgc tgatgagctt aaaaaccgcc gtgcgacact tgtcaattta caacgtattg
     1921 ataaagtatc agcgaaaaga attattgatt ttttaagcgg tactgtttat gcaatcggtg
     1981 gagatatcca acgtgtaggt actgatattt tcttatgtac gcctgataat gtggaagtag
     2041 ctggaagcat tacagaccat attgaaaata tggaacattc attcgactaa ggagttaaca
     2101 tatggatata aatgtgctag ctacaatatt taaattcatc ctttttgttg ttgaaattta
     2161 ttatttcggc atgattatat atttctttac atcttgggta ccaagtatta gagaaactaa
     2221 ggtaggttat tttttagcga aaatatatga acctttctta caaccattta gaaaagtaat
     2281 tccacctatt ggaattatcg acatatcatc aatcgctgca attatcgttt tagtattatt
     2341 ccaaaaaggg ttactccaaa tctttaattg gattttaatt caattacaat aaattaagcg
     2401 acataacgag gcaagggtac atgatacttt agcctcgttt ttgatatgca tttttctgaa
     2461 tataagggca atagatggta ttttataatt tttttaaggt agtgattaac atagatattt
     2521 atcaacactt tagacaggaa gaatacgaat taattgatca gctaacggat aaatgtgatc
     2581 aagcggaaca gcattatgca ccagtattaa cgcatttttt agatccaaga gggcaatata
     2641 tattggaagt gatttgtggc agttatgaag atttaaacgt atctttttat ggtggaccta
     2701 atgctgaaag aaaaagagca atcatttcgc cgaactatta tgaacctaaa gaaagcgact
     2761 ttgaattaac tttaatggaa atagattatc ctgaaaaatt cgtcacttta aaacatcaac
     2821 atattttagg gacattaatg tctttaggta tcgaacgcga acaagttgga gatataattg
     2881 tgaatgaacg aattcaattt gttttgacaa gtagattgga atcatttatt atgttagaat
     2941 tacaacgtat taaaggcgca tcagttaaac tttatactat tccagtaaca gatatgatac
     3001 aatctaatga gaattggaaa aatgaaagtg caacagttag ttctttaagg ttagatgttg
     3061 ttattaaaga aatgatacgt aaatcacgta cgattgcgaa acaactaatc gaaaaaaaac
     3121 gtgttaaagt gaatcacact attgttgatt cagcagattt tcaattacaa gcaaatgatt
     3181 taatatccat ccaaggtttt ggtagagcac acattactga cttaggtggt aaaactaaaa
     3241 aagataaaac gcacattacc tatagaacat tattcaaata gtaatgattt aaggaggata
     3301 acaaatgcct tttacaccaa atgaaattaa gaataaagag ttttcacgtg taaagaatgg
     3361 tttagaacct actgaagttg ctaatttttt ggagcaacta agcactgaaa ttgaacgtct
     3421 taaagaagat aaaaaacaac ttgaaaaagt aatcgaagag agagatacta atattaagtc
     3481 ttatcaagac gtgcatcaat ctgtaagtga tgctttgata caagctcaaa aagctggtga
     3541 agaaactaag caagctgcag agaaacaagc tgaagcgatt atagctaagg cagaagcgca
     3601 agctaatcaa atggttggtg acgcggtaga aaaagcacgc cgtttagcat tccagactga
     3661 agatatgaaa cgtcaatcaa aagtatttag atcgcgtttc cgtatgttag ttgaagcgca
     3721 attagactta ttaaaaaacg aagattggga ttacttgttg aattatgatt tagacgctga
     3781 acaagtgacg cttgaaaata ttcatcattt gcatgaaaat gatttaaagc cagatgaagt
     3841 tgcagcaaat gcacaaaata atgcatcaaa tacaccagac aataatcaac aatccaatga
     3901 ttcagaaaca actaagaagt aagaattaaa taaagacaga cgcgtaatat acatttaact
     3961 tttcacagcg aattaggtaa tggtgagagc ctagtaaaag catgtatgtt atatcactgg
     4021 ctttttaata tttaaataat gtaatgagag aactctaagt tgagttaata agggtggtac
     4081 cgcgagcaat cgtccctttt aatttaactt agagtttttt aaatttttaa ggagtgaaaa
     4141 aaatggatta caaagaaacg ttattaatgc ctaaaacaga tttcccaatg cgaggtggtt
     4201 taccaaacaa ggaaccgcaa attcaagaaa aatgggatgc agaagatcaa taccataaag
     4261 cgttagaaaa aaataaaggt aacgaaacat tcattttaca tgatggccca ccatacgcga
     4321 atggtaactt acatatggga catgccttga acaaaatttt aaaagacttt attgtacgtt
     4381 ataaaactat gcaagggttc tatgcaccat acgtaccagg ttgggataca catggtttac
     4441 caattgaaca agcattaacg aaaaaaggtg ttgaccgaaa gaaaatgtca acagctgaat
     4501 tccgtgagaa atgtaaagaa tttgctttag aacaaattga attacagaaa aaagatttta
     4561 gacgtttagg tgttcgtggt gactttaatg atccatatat tacattaaaa cctgaatacg
     4621 aagctgcaca aattcgtatt tttggagaaa tggcagataa aggtttaatt tataaaggta
     4681 aaaagccagt ttattggtct ccttcaagtg agtcttcatt agcagaagca gaaattgaat
     4741 atcacgataa acgttcagca tcaatttacg ttgcatttga cgttaaagat gacaaaggtg
     4801 tcgttgatgc agatgctaaa tttattatct ggacaacaac gccatggaca attccatcaa
     4861 atgttgcgat taccgttcat cctgaattaa aatatggtca atacaatgta aatggcgaaa
     4921 aatatattat tgcagaagcc ttgtctgacg ctgtagcaga agcactggat tgggataaag
     4981 catcaatcaa attagaaaaa gaatacacag gtaaagaatt agagtatgtt gtagcacaac
     5041 atccattctt agacagagaa tcgttagtga ttaatggtga tcatgttact acagatgctg
     5101 gtacaggttg tgtacataca gcaccaggtc acggggaaga tgactatatt gttggtcaaa
     5161 aatatgaatt gccagtaatt agtccaatcg atgataaagg tgtatttact gaagaaggcg
     5221 gccaatttga agggatgttc tatgataaag ctaataaagc cgttactgat ttattaacag
     5281 aaaaaggtgc actattaaaa ttagacttta ttacacatag ctatccacac gactggagaa
     5341 caaaaaaacc tgtaatcttc cgtgctacac cacaatggtt tgcctcaatc agtaaagtaa
     5401 gacaagatat tttagatgca atcgaaaata caaacttcaa agtaaattgg ggtaaaacac
     5461 gtatttacaa tatggttcgt gaccgtggcg aatgggttat ttctcgtcaa cgtgtgtggg
     5521 gtgtaccgtt accagtattt tatgctgaaa atggcgaaat tatcatgacg aaagaaacag
     5581 tgaatcatgt tgctgattta tttgcagaac acggttcaaa tatttggttt gaaagagaag
     5641 cgaaagactt actaccagaa ggatttacac atccaggcag ccctaacggt acatttacta
     5701 aagaaacaga cattatggac gtttggtttg attctggttc atcacaccgt ggcgtgttgg
     5761 aaacaagacc ggaattaagt ttcccagcgg atatgtattt agaaggtagt gaccaatatc
     5821 gtggttggtt caactcttct atcacaactt cagttgctac aagaggagta tcaccttata
     5881 aattcttact ttctcatggt tttgttatgg acggtgaagg taagaaaatg agtaaatctt
     5941 taggtaatgt gattgtacct gaccaagtgg ttaaacaaaa aggtgctgat attgcgagac
     6001 tttgggtaag tagtacggac tatttagctg atgttagaat ttctgatgaa attttaaaac
     6061 aaacatctga tgtttatcgt aaaatcagaa atacattaag atttatgtta ggtaacatta
     6121 acgatttcaa tcctgacaca gatagcattc ctgaatcaga gttattagaa gtggatcgtt
     6181 acttgctaaa tcgtttacgt gaatttactg caagtacgat taacaactat gaaaactttg
     6241 actacttaaa tatttatcaa gaagttcaaa actttatcaa tgttgagtta agtaatttct
     6301 atttggatta cggtaaagat attttatata ttgaacaacg tgattctcat atccgtcgta
     6361 gtatgcaaac agtgttatat caaattttag ttgatatgac gaagttgtta gcaccaatct
     6421 tagtgcatac agctgaagaa gtttggtctc atacaccaca tgttaaagaa gaaagtgttc
     6481 acttagcaga catgcctaaa gttgtagaag tagatcaagc tttattggat aaatggcgta
     6541 catttatgaa tttacgtgat gatgtgaacc gtgcattaga aactgctcgt aatgaaaaag
     6601 ttattggtaa atcattagaa gctaaagtta cgattgctag taacgataaa tttaatgcat
     6661 ctgaattctt aacttcattt gatgcattac atcaattatt tatcgtgtca caagttaaag
     6721 ttgtagataa gttagacgat caggcaacag cttatgaaca tggtgatatt gtcatcgaac
     6781 atgcagatgg tgaaaaatgt gaaagatgtt ggaactattc agaggatctt ggtgctgttg
     6841 atgaattgac gcatctatgt ccacgatgcc aacaagttgt aaaatcactt gtataattga
     6901 aattgtataa agtactcata cagatgatat aaattaaagc tctcttcata atcatgttgt
     6961 agtttttgtt gacatgatga agagagtttt tttgtgaata aaaaaatgac caagttaccg
     7021 gtcatatatg taaaaaatgt gcgatttact aaaataaaaa ttattcagga atggtacaaa
     7081 ttctctgagg catataaatg cgttatagtt gctattctca attatgttcg cgataatttt
     7141 aagtaaaagt aagcacagat attgaatttg ataggagtta attgaatgta tcataacagt
     7201 aacgcaaact ttgtcaatgg tatcacttta aatgtgagag ataagaatga attaaagcca
     7261 ttttatgagg acatattagg attaaatatt ataaatgaga cattaacatc gatacaatat
     7321 gaagtaggtc aaaataatca tgtcattaca cttgttgaat tacaaaatgg acgtgaacct
     7381 ttaatgtccg aagcgggact gtttcatatc gcaattaaac tacctcaaat tagtgattta
     7441 gctaatttac taattcattt aagcgaatat gatattccag ttaacggagg tatacagcct
     7501 gcttcgttat cattattttt tgaagacccg gaaggaaacg gttttaaatt ttatgttgat
     7561 aaagacgaag cgcaatggac gaggcaaaat aatttagtaa aaattgatat tagaccatta
     7621 aatgtaccga gattagtgag tcatgcaaca aaattgttat ggttaggtat tccagatgac
     7681 gctattatag gtgcattgca tattaagaca attcatttat cagaggtaaa agagtactac
     7741 ctcgattatt ttggattaga gcaatcggca tatatggatg attattcaat atttttagca
     7801 tcgaatggct attatcaaca tttggccatg aatgattggg tatcagcaac gaaacgtgta
     7861 gaaaattttg atacgtatgg attagcaatt gttgactttc attatcctga aacaacacat
     7921 ttaaatttac aaggtccgga tggtatctat tatcgcttta atcatatcga agttgaagat
     7981 tagtatatac tttgaatgga cgaaccatat aatgaatcgt ttttaatgat ctttttatac
     8041 aagttatgaa ggaggctggg acattaagtt cttaggcaat gtaaaaagct gatttctatt
     8101 aattatttga tagaaatcag cttttttgat atgtatttta taatgtacag ctcgttgagc
     8161 tgctattttc cttatattaa ttgccattaa tacaaaacct agctct
//
LOCUS       DANJFZ010000115         8157 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.115, whole genome shotgun sequence.
ACCESSION   DANJFZ010000115 DANJFZ010000000
VERSION     DANJFZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.115"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..911
                     /locus_tag="PWN45_002273"
     CDS             <1..911
                     /locus_tag="PWN45_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HDL0624018.1"
                     /translation="NDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIH
                     IDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYS
                     ILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDF
                     KFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPAN
                     NFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDK
                     DQETFVLSIVKEIVKTQKHWCGDKND"
     gene            904..2148
                     /locus_tag="PWN45_002274"
     CDS             904..2148
                     /locus_tag="PWN45_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HDL0624019.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            2164..2682
                     /locus_tag="PWN45_002275"
     CDS             2164..2682
                     /locus_tag="PWN45_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HDL0624020.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            2692..4065
                     /locus_tag="PWN45_002276"
     CDS             2692..4065
                     /locus_tag="PWN45_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL0624021.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            4088..4780
                     /locus_tag="PWN45_002277"
     CDS             4088..4780
                     /locus_tag="PWN45_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HDL0624022.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            4777..5538
                     /locus_tag="PWN45_002278"
     CDS             4777..5538
                     /locus_tag="PWN45_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDL0624023.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            5535..6233
                     /locus_tag="PWN45_002279"
     CDS             5535..6233
                     /locus_tag="PWN45_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HDL0624024.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(6707..7273)
                     /locus_tag="PWN45_002280"
     CDS             complement(6707..7273)
                     /locus_tag="PWN45_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDL0624025.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
BASE COUNT         3000 a         1030 c         1339 g         2788 t
ORIGIN      
        1 agaatgatca tgtgtatgaa attgttacac caatttataa aaaatcatct tatcgaggac
       61 ccgaaatcga aattccaaaa tataaaaata cagatattga atatgacaaa gactggtttg
      121 cattacatat tcatatcgat aagccatcgc aagatacatt tatcatcgac aatttgtatc
      181 catttgtaaa acatcttaaa gataagggag atatagatca atatttttta atgcgttata
      241 tcaaacaagg tgacatttta aagttgaggt tattcaggaa tgacgaaaat tacgcagaaa
      301 tatacagcat cttgaagaat tggttaccac atgttcgaca aacgacagaa gtttcagatt
      361 atgaatttgt atcatacgag ccagaatttt ttagatatgg tggaaagaac acgattaatg
      421 aaatagaagc cttctttgaa tatgacacga atttagctgt caatattatt gaaaatgatt
      481 ttaagtttga tcgaccatat attgttgcta tttcaataat gtatttattt gagatgtttt
      541 caatttctaa tgaagagcgt atggaaattg ttaataacta tgtacctaca tcatttaaaa
      601 gtaaagatat tagacctttt aaaaatgaac ttgtaactat atgtaatcct gctaataatt
      661 ttgaatatat ggctaaacac tatagtggta tttatcgaat tttaaaagat ggcaaccaaa
      721 ttttgagcaa attaaatgaa gggcttaaaa aaactttaac tactaaaagg tcaaggatta
      781 ttggcagctt aattcatatg cgctgcaatc gaatatttgg cattgataag gatcaagaaa
      841 catttgtttt atctattgta aaagaaattg ttaaaactca aaagcattgg tgtggtgaca
      901 aaaatgattg atatgaatct aatattgaag aagaagtttc aagagatttc agaagtagat
      961 gatttcattt caaaagcatc tactgaaacc gattattttg aaccttcaac tttatcacat
     1021 ggtataccag gtatcatttt gtttttagat gcttatcaaa aagttttcga tattaatact
     1081 gagcaaattg tgcataagta catcatgaaa ttagcaccat atttacagag tggtcaatat
     1141 aacaattctt tgtttggtgg actttcaggt attgcatttt caatggattt agcatcgcaa
     1201 aatggcaaaa attatcaaaa tattttaaat aatattgatg gtcacattgt aaatgaaata
     1261 gaaaataaat ttgatcaaat attacaagaa ccattaaatc cattaaatta tgatactgtc
     1321 agtggattag ctgggatagg gagatatttg ctaaatagag tagatgagaa tgaatttaat
     1381 gttaaagcat taaaaagcat attagtatac tttaaagata ttcaatattc taaaaatagc
     1441 tgggtagtcc cacaagaaag tcaattttta gagtctgata aaaattattt tactgaaggt
     1501 aatatcaatc ttggccttgc acatggagtg ctaggaccga tgtctttatt tgcattatgc
     1561 gtgattaaag gaattacgat tgaaaatcat cagcacatat taaaagacat gtacaaattt
     1621 atcatggacg aaaaattttg taaccacgaa agatggttgc agcgttacga tttaatttct
     1681 gaacgtaatc atttcaattt tattcggaat ggttggtgtt atggcaatac gggtgtaatg
     1741 acgacgttgt ttttaatcgg ccaagcatta caagatgatg aaataattaa aatgtctaaa
     1801 aaagtgatgc tacaagtagt aaatgataaa gatgaaaatt taataagtcc aactatttgt
     1861 catggattgt catcacaaat attaatgtta acaattatga atttgaattt tgaattaaat
     1921 gaagtgtctg attatatcac tgtattaata aataaactga tttctcatta taaggaagat
     1981 tatctggtga attttataga cattaatgaa aataagcaag atgtatttaa aagtaggaaa
     2041 gttggccttt tagaaggtga attaggggtc tatctaacat tgatgacact gaaaaataca
     2101 aaaattctca atgaaaaaaa ttggaccaat gcgtttttaa ttagctgaat ggaggcgtat
     2161 atcatgggag aaaatgtatt gatttgtttg tgtggctcag ttaatagtat caatattagc
     2221 cactatatta ttgaattaaa atcaaaattt gatgaagtaa atgtaatcgc gtcaacaaac
     2281 ggtagaaagt ttattaatgg tgaaatatta aagcaatttt gcgataacta ttatgacgaa
     2341 tttgaagacc cttttttaaa tcatgtagat atagcaaata aacatgacaa gattattatt
     2401 ttacctgcga cttctaatac gattaataaa attgcaaatg gtatatgtga caatttatta
     2461 ttaactattt gtcatacagc ttttgaaaaa ctttctatat ttccaaatat gaatttacga
     2521 atgtgggaaa atccagttac tcaaaataat attcgattat taaaagatta tggtgtatca
     2581 atatatccag caaatatttc agaaagttat gaattagcgt caaaaacatt taaaaagaat
     2641 gttgtcgcac ctgaaccata taaagttctg gaattcattt gagattagaa tatgaaaatc
     2701 ataaagcgcg ctattattag tttaataata ttgagtcttt taatatctat tacaatgagt
     2761 aatgcatcag cgtcagaaga actatattac agtgttgaat acaaaaatac agcaacattt
     2821 aacaagttag ttaaaaagaa gtccttaaac gttgtctata atataccgga attacatgtg
     2881 gcacagatta aaatgacgaa aatgcatgct aatgctttag caaactataa aaatgatatt
     2941 aaatatatca atgccacatg ttcaacttgt attactagcg agaaaacaat agacagaaca
     3001 tcaaatgagt cattattttc aagacaatgg gatatgaata aaataaccaa taatggtgca
     3061 tcgtatgatg atttgccaaa acatgctaac accaaaatag caatcataga tacaggtgtg
     3121 atgaaaaacc atgacgattt gaaaaataat ttctcgactg attctaaaaa tttagtacct
     3181 ttaaacggtt ttagaggtac tgaaccggag gaaacaggtg atgttcacga tgtcaatgat
     3241 aggaaaggac atggcacgat ggtgtcgggt caaacgagtg ctaatggtaa gttaataggt
     3301 gttgcaccga ataacaaatt tacaatgtat cgcgtgtttg gtagtaaaaa aacagaactg
     3361 ctttgggtat caaaagcgat tgttcaagct gcaaatgatg gaaatcaagt cattaatatt
     3421 agtgttggta gttatattat tttggacaaa aatgaccatc aaacatttag aaaagatgaa
     3481 aaagtagaat acgatgcgtt acagaaagca atcaattacg ccaagaagaa aaaatctatc
     3541 gttgttgctg cagctggtaa tgatggtatt gatgtcaatg acaaacagaa actaaaatta
     3601 cagcgtgaat atcaaggtaa tggcgaagtg aaagatgttc ctgcatctat ggacaatgtc
     3661 gttacagtag gatctacaga tcaaaagagt aatctatctg agttttccaa ttttggtatg
     3721 aattatacag atattgctgc gcccggagga tcatttgctt atttaaatca attcggtgtg
     3781 gataaatgga tgaatgaagg gtatatgcat aaggagaaca ttttaactac tgccaataac
     3841 ggaagatata tttatcaagc tggaacttca ttagccacac ctaaagtttc gggagcacta
     3901 gctttaatca ttgataaata tcatcttgaa aaacatccag ataaagcgat tgaattgtta
     3961 tatcagcatg ggacatctaa gaataataaa ccatttagta gatatgggca tggtgagctt
     4021 gatgtgtata aagcattaaa tgtagcaaat caaaaagcaa gttaataaat caaaggagtt
     4081 tttgattatg gcaaaattag ttactgaaaa catttcgaag cggtttaaaa atcaagatgt
     4141 attaaagcat attaatatca ctttagaaaa taacgaagtt tatggattac ttggtattaa
     4201 tggagccggt aaaacgacac ttatgaaaat tatatgtggc atacttcaac aagattcagg
     4261 ggaaattaaa ttagataata gaccaatgac acgaaatgat ttgcacaaag ttggttcgct
     4321 tattgaaaca cctgcgacat ataatcattt aagtgcacaa gataatttga aaattgtgtg
     4381 tttaaatgaa agcgttgatt tcagcgaaat taatagtgtt ttaagcttag tcaatttaaa
     4441 tgtcgataaa aagaaaaagg ttaaggactt ttctttaggt atgaaacaaa gacttggaat
     4501 tgcaatggca ttaattaaaa agccagaaat tttagtatta gacgaaccat ctaatggttt
     4561 agacccatat ggaatccaag aacttagaga acttctaaaa ttattaacag aacaaggtac
     4621 tagtattatt atttcaagtc acattttatc tgaaatccaa gttttagcag atcatatcgg
     4681 tattattcat gagggtgagc taaaatatca gcaaagaaat aacaaagatg aaaacttaga
     4741 agagatattc ttcaaaataa cgaaaggtga ttacaaatga tacatttaaa gatagaaggt
     4801 atcaaattta aaaattcttt cagtatgtat gttttattaa taagtccgct ggtatttctt
     4861 tgttttgcta ttttcacagt cttattcgcc aaaagtaata cgggaacagc gaatagtgtg
     4921 tcaccatata taactttact atttaatatt tggccaattg ctttcatccc gattgtatta
     4981 tgtatggctt gtaattcgtt atttaaaatt gaaatgagaa ataaatcatt taattattac
     5041 ttaagtaata attggtcgat tacaaaagaa ataagagcaa agattttcat tttatcaata
     5101 gcatttttgg tacattgctt tttagtattt attattgctt atataggtga cctaattatt
     5161 aatccacatc cggttaatgc tatgttgtta ttggtgacaa tattgttgat gtatgtagta
     5221 tctctaccat tgataccgct caacttttta ttaactcgat actttggtgt gttcgtatca
     5281 atattaataa acttagtatt atcagtcatt tgtgtcttgt ttttaacatt gaagagttta
     5341 ttttgggtgt tgccgtgggg gataatgcag agaatcccgc ttattacgct tggtatacta
     5401 cctaatggtt tagttgtaaa ccataattca aaatacttta atgatctcaa tgccttatat
     5461 atttcgatta ttgttagcat cattattttc gcgatagtaa catttttaaa taataagaaa
     5521 agttggcgat taaaatgata attaacgaat taaagtcatg taagttgaaa ttttctaagc
     5581 aagcgctcac atttgtaccc attattgtaa ccatattgtt tatattattt ataaattggt
     5641 atttaaacgt aaatttatgg aatggtcgac aaataagttt gtttacagcg agttttaatg
     5701 caattacatc gctattaatt tctataaacg tctatcaagt tatcaatttt gaagaaaata
     5761 ttggtcactt taatcatatt ttaggaaaag ctaataggct aaattggtta aatgcatcaa
     5821 tgatttttac ttatactatt acagccatat gtattctatt agcatcaatt aatttattgt
     5881 ggcattcaca tgatatgaaa ataacactta tgtttatagg cgtatcattg tttttcaatg
     5941 taattatatt actgctactt tttattttta gtattttcat taaagatgta atggctattg
     6001 ttgtcggagt tttaatgttt atttttaacg tttattttgg attagaagtg cttggagatc
     6061 attcgtggtt ctatttacca atcacatatg ctacacgtta cgtttatatg tttatcgaag
     6121 ggagtatacc agttacatta acattggcaa tctatattat tatcacgttg ctattagcag
     6181 ttttactctt taaaggattt aataaatgga gcggtagaac aattaaagat taaagattgg
     6241 ggctctaacg gtaaaagaaa tttagttgcg ttgaaaaggg aggaaagaga acagttaaat
     6301 atgaatacaa aataattttg ttttcggaca gcaggggtat tagacgcgat tgacaatgtc
     6361 tgtttaatta aacgtaatgt ttatttaagc gatgaatatt aggtgaaaag tttttgaatt
     6421 tgaatgtaat tgaggtttat tgattagaca ttttattgaa ttgcgtgtta ttatataaat
     6481 gtaaaaataa gacgacatgc gcgaacatgt cgtctaagca agccaaacgc tggcttctaa
     6541 aatatttttt taaccattcc aacttgccaa agttaatgcg gaatggtttt tttattttcc
     6601 gctaattgaa ataaaaatga cgttttaata tattatgggc taggtggttt gtaagaaagg
     6661 gttagttatt aatgttttat gaattaagga aatttgagtt taaggtttaa tcaattgtga
     6721 ttttgttgat gaagcgttta gttagagtat tttcgccacc actagttact ccttctccca
     6781 ctttacccga gactggagaa gagctatctg aagaataaat agatactttt tggccatttt
     6841 gtagtaaacc aagacctttt aacttctcgg ttagagaatt ccatgtattt ttagcatcta
     6901 gctttttgtt aaagtcatcg tagacatttt ccttagttaa atcaatttgt tttaatcctt
     6961 taaagtctat agattgtgtt agatggcctc catcatcatt tccaggagca gaaacgctgc
     7021 tagaatatcc attgcttaat aaataagtaa cgttgattgt ttcgtactcg ttacttaaaa
     7081 taatatcaga atcatgtaat gaatctttaa cttttttcca taattgacca tctgtcattt
     7141 tttcttctgc tttagccgtt ttaacaactt tatttgtatc taatcctaag tatgaagaat
     7201 gtaagcctgt tcctaatgtt gttaatacta aagcacttgc tactaatgtt ttacctaaaa
     7261 attttgtatt catttttatt gctccttttt ttatattgta aacgtttaca atgaaaatat
     7321 aataataatt ttttaaaaga acaattaact aaatatcaaa aatgtattaa ctatctatta
     7381 actaaaaaat agaataattt tttaacatag ttttgttgtt ttgaattata aaaactaaag
     7441 taaaaatgtt ggatgtgaaa gattgttgat gagaattttg agatgtagat aaatatcaaa
     7501 ctagtctata ggtaaaaatt tataattaat ttgaaaagaa tgtctcaaat aatgtttgaa
     7561 ttagcaggtg tcatactaag taatgttaag taaacatttt atactttatc agaacaaaaa
     7621 agacatgcat caacatgtca gtgaaactaa tccatattat cttaacaacg aaattgaatc
     7681 gaacctgaaa aacgtattta caaatcgcaa ctactctatc caaggcaaaa tcgcaaaatc
     7741 tgatctccga aataacatat ctcgaaaaaa attccataat tcaatcatct tcaatattta
     7801 acaatacatt actagttcca aatatactca tacatacatg tttcattgat tgactgcata
     7861 ttgtcataaa taatagggga actaatcgct aataatgttg ttaaacacaa tacatagtaa
     7921 agtgaaagat tataaataca tgaattgttc accattccgc catatatttt caatcaccta
     7981 atttccgaat aaatattaca attttaattc aataattaat atttaagacc aaaataatcc
     8041 taaaaagtca attttaattg cgtgatattg agttttaagc atatttaaat aaatgagtca
     8101 attcatgtaa aataatttaa taaaacgtta ataagtggtt aattaatatt taattaa
//
LOCUS       DANJFZ010000116         8131 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.116, whole genome shotgun sequence.
ACCESSION   DANJFZ010000116 DANJFZ010000000
VERSION     DANJFZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.116"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            31..681
                     /locus_tag="PWN45_002281"
     CDS             31..681
                     /locus_tag="PWN45_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0624026.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            678..914
                     /locus_tag="PWN45_002282"
     CDS             678..914
                     /locus_tag="PWN45_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDL0624027.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1183..3168
                     /gene="uvrB"
                     /locus_tag="PWN45_002283"
     CDS             1183..3168
                     /gene="uvrB"
                     /locus_tag="PWN45_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL0624028.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3176..6022
                     /gene="uvrA"
                     /locus_tag="PWN45_002284"
     CDS             3176..6022
                     /gene="uvrA"
                     /locus_tag="PWN45_002284"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL0624029.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6684..7616
                     /locus_tag="PWN45_002285"
     CDS             6684..7616
                     /locus_tag="PWN45_002285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL0624030.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7622..>8131
                     /gene="lgt"
                     /locus_tag="PWN45_002286"
     CDS             7622..>8131
                     /gene="lgt"
                     /locus_tag="PWN45_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0624031.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMY"
BASE COUNT         2934 a         1108 c         1655 g         2434 t
ORIGIN      
        1 tatgattaat taatggagga ggtaattgaa atgggtgtac atcaatattt taaaagatta
       61 tcagatatgg aaagacttat aagattacct ggaaaattta aatattttga acacaatgtt
      121 gcagcacact cctttaaagt aactaaaatt gcacaatatc tagcaacagt tgaagaatat
      181 catggacgaa agattaattg gaaaagctta tatgaaaaag cattaaatca tgatttcgcc
      241 gaagtgttta ctggtgatat aaaaacacct gttaaatatg cgagtagtga attaaaaaaa
      301 ttattttcgc aagttgaaga agaaatggta gagaccttta ttgaagaaga aattccatta
      361 caatatagag atgtttataa gcaacgactg caagaaggta aagatgattc attagaaggc
      421 caaatacttt cagttgctga taaaattgat ttgctttatg aaacatttgg agaaatacaa
      481 aaacgtaatc ccgaagaatt atttttcgaa atttatgaaa tgagtctaga aacaattatt
      541 caatttgacc atttagcatc tgtacaagat tttattaata atatcattcc agaaatgttg
      601 actgaaaact ttatacctag aacagaatta agagaaacaa ccatgaacat tttaaataaa
      661 agaaaagagg aaaatgaatg atatggtatt ttagcgcagc attctttcca tgtgtcttgg
      721 tagtattatt tagtgtaata acaagaagta aatgggtcgg tactattctg acattaattt
      781 taattggtgc ctcaatctat aaagagtatt tccataacga gtggattatt tttattgatg
      841 tagtgtcatt attagctggt tatttaatta tagatcaact cgaatttcat aaacatcaag
      901 atgaagatcg ctaagattaa ctttaaaata atgtttcaaa caaatttgtt gaaacaaaat
      961 gatgattaat ataatgtgta tttacatact aaaaataaca agataaacga tttgatttaa
     1021 ggcaagcata gttagcacaa ctatgtttgt ttttctttgt tcgacatttt tacgaacaaa
     1081 cgtttgcttt ttgtgtgact actttgctaa aatatgtaat gagaaagcaa agagttgagc
     1141 ggaaatataa ataatacgtt gaaagaggag gcatatgtga caatggttga acattatcct
     1201 tttaaaatac attctgattt tgagcctcaa ggtgaccaac cgcaagcaat taaagaaatc
     1261 gtggaaggta ttaaagcggg gaaaagacat caaactttat taggtgctac tggcacaggg
     1321 aaaacattta cgatgagtaa tgttattaaa gaagttggga aaccaacgtt aattatcgcg
     1381 cataacaaaa cattagcagg acaattatat agtgagttta aagaattttt tcctgaaaac
     1441 agggtggaat actttgtaag ttactatgat tattatcaac cagaggcata cgtaccgtct
     1501 actgacactt ttattgaaaa agatgcctca atcaatgatg aaattgatca actacgacat
     1561 tctgctacaa gtgcattatt tgaacgcgat gatgtaatta ttattgctag tgtaagttgt
     1621 atatatggtt taggtaatcc tgaagaatat aaagatttag tagtaagtgt tcgagttggt
     1681 atggaaatgg atagaagtga attacttaga aaacttgtag atgtgcaata tacacgaaat
     1741 gacatcgatt tccaacgagg aacgtttcga gtgcgtggtg atgtagtgga aatattccca
     1801 gcctctaaag aagaactttg tataagggtt gagtttttcg gcgatgagat tgaccgtatc
     1861 cgagaagtta actacctaac aggtgaagtg ttgaaagaaa gagaacattt tgcgatattc
     1921 ccagcttctc acttcgtaac acgtgaagaa aagttgaaag ttgcgattga acgtattgaa
     1981 aaagaattgg aagaacgatt gaaagaatta cgagatgaga ataaattact agaagcgcaa
     2041 aggttagaac agcgtaccaa ctatgattta gaaatgatgc gagagatggg attctgttca
     2101 ggaattgaaa actattccgt acatttaact ttgcgaccac tgggttcgac accatatact
     2161 ttattggatt actttggcga tgattggtta gtaatgattg atgaatcaca tgtgacatta
     2221 ccgcaagttc gaggcatgta taacggagac agagcgcgta aacaagtttt ggtggatcat
     2281 gggtttagat taccgagtgc attagataac cgtccactta aatttgaaga atttgaagaa
     2341 aagacaaaac aacttgtgta tgtatctgca acgcctggac catacgaaat tgaacatacg
     2401 gataagatgg ttgaacaaat tattcgtcct actggtttac tggatcctaa gattgaggtt
     2461 agacctactg aaaatcaaat tgacgattta ttaagtgaaa ttcaaacaag agttgagcgt
     2521 aatgaacgcg tacttgttac aacgctcact aaaaagatga gtgaagattt aaccacatac
     2581 atgaaagaag cgggtattaa agttaattat ctgcattcag aaatcaagac attagaacga
     2641 attgaaataa ttagagactt acgaatgggt acatatgatg ttatcgtagg tattaattta
     2701 ttaagagagg gtattgatat accagaagtt tctctagttg tcatattaga tgcagataaa
     2761 gaagggtttt tacgttctaa ccgctcatta attcaaacaa taggtagagc tgcgcgtaac
     2821 gataaaggtg aagtcattat gtatgccgat aaaatgactg attcgatgaa gtatgcaatt
     2881 gatgagacac aacgtcgtcg agaaatacag atgaaacata atgaaaaaca tggtattaca
     2941 cctaaaacaa ttaataaaaa aatacatgat ttaattagtg ctactgttga aaatgacgaa
     3001 aataatgaca aagcacaaac tgtgatacct aagaagatga cgaaaaaaga acgtcaaaag
     3061 acaatcgaca atatagaaaa agaaatgaaa caagcagcga aagatttaga tttcgagaaa
     3121 gctacagaat taagagatat gttatttgaa ttaaaagcag aagggtgaca agtaaatgaa
     3181 agaaccatcc atagtagtaa aaggtgctcg tgcgcataac ttgaaagata ttgatatcga
     3241 actacctaaa aataaattaa ttgttatgac aggtttatct gggtcaggta aatcgtcatt
     3301 agcattcgat actatatatg ctgaaggaca acgacgttat gttgaatcat taagtgccta
     3361 tgcgcgtcaa tttttaggcc aaatggacaa accagatgtt gatacaattg aaggattatc
     3421 gccagcaatt tcaatagatc aaaaaacaac aagtaaaaat ccaagatcaa ctgtagcaac
     3481 agtaacagaa atatatgatt atatacgttt gttatatgca cgtgttggta aaccttactg
     3541 tccaaatcac aatatagaaa ttgaatcgca aacagtacaa caaatggttg accgcattat
     3601 ggaattagag gcacgtacaa agattcaatt attagcacct gtcatcgctc atcgtaaagg
     3661 tagtcatgaa aagctaatcg aagatattgg taaaaaaggt tatgtacgtt taagaatcga
     3721 tggcgaaatt gttgatgtaa atgatgtacc tactttagat aagaacaaga atcatacaat
     3781 agaagttgtt gtagaccgat tagttgttaa agatggaatt gaaacacgac tagctgactc
     3841 tatagaaact gccttagagc tttcagaagg acaattaaca gtcgatgtca ttgacgggga
     3901 agaccttaag ttttcagaaa gccatgcttg tcctatatgt ggattttcaa tcggagagtt
     3961 agaaccaaga atgtttagct ttaacagtcc ttttggtgct tgtccgacat gtgatggctt
     4021 aggccaaaag ttaacagtcg atgtagactt ggttgttccc gacaaagata agacgctaaa
     4081 cgaaggtgca atagaacctt ggataccgac gagttctgat ttttatccaa cattgttaaa
     4141 acgtgtttgt gaagtttata aaatcaatat ggataaacct tttaaaaagt taacagaacg
     4201 tcaacgtgat attttattgt atggttctgg tgacaaagaa attgaattta catttacaca
     4261 acgtcaaggt ggtactagaa aacgaacaat ggttttcgag ggtgtagttc ctaatataag
     4321 tagacgattc catgaatctc cttcagaata tacacgtgaa atgatgagta aatatatgac
     4381 tgaactacct tgcgaaactt gtcatggaaa gcgattgagt cgtgaagcgt tatctgttta
     4441 tgtaggtggt ttaaatattg gtgaagtagt cgaatattca atcagtcaag cgctgaacta
     4501 ttataaaaac attgatttgt cagaacatga tcaagcgatt gcaaatcaaa tattgaaaga
     4561 aattatttcc cgactcactt ttttaaataa tgtgggactt gaatatttaa cgttaaacag
     4621 agcttcaggt acactttcag gtggtgaagc acaacgtatt cgattagcaa cgcaaattgg
     4681 gtcgcgtttg actggtgtct tatatgtatt agatgagcca tcaattggac tgcatcaaag
     4741 agataatgat cgattaatta atacacttaa agaaatgaga gatttaggaa atactttaat
     4801 tgtagttgaa cacgatgatg atacaatgcg tgcggctgat tacttagtgg atataggtcc
     4861 tggtgctggt gaacatggag ggcagattgt gtctagtggt actcctcaaa aggtaatgaa
     4921 agataaaaaa tcattaacag gacaatactt gagtggtaag aaacgtattg aagtacctga
     4981 atatcgcaga ccggcttcag atcgtaaaat ttctatacgt ggagctagaa gcaacaatct
     5041 taaaggggtt gatgtggaca taccactatc aatcatgacg gttgttacag gtgtatcagg
     5101 ttctggtaaa agctcattag taaatgaagt attatacaaa tcattagctc aaaaaattaa
     5161 taaatctaaa gtaaagccag gattgtacga taagattgaa ggtattgatc aacttgataa
     5221 aattattgat attgatcaat caccaatagg tagaacgcca cgctctaatc cagcaacata
     5281 tactggtgtg tttgatgata tacgtgatgt gtttgcgcaa acaaatgaag ctaaaattcg
     5341 aggatatcaa aaagggcgtt ttagttttaa tgtaaaaggt ggacgctgtg aagcttgtaa
     5401 aggtgacggt attattaaaa ttgaaatgca ttttttacct gatgtttatg ttccttgtga
     5461 agtgtgtgat ggtaaacgat ataatcgtga gacactagag gttacttaca aaggtaaaaa
     5521 tattgctgac attttagaaa tgactgttga agaagcaaca caattttttg aaaatattcc
     5581 taagattaag cgcaagttac aaacactagt tgatgttggt cttggatacg tcacattagg
     5641 tcaacaagct acaacgttat caggtggtga ggctcaacgt gtgaaacttg catctgaact
     5701 tcataaacgt tcaactggta aatctattta tatcctagat gaaccgacaa cagggttaca
     5761 tgttgacgat attagtagat tattaaaagt attaaaccga ttagttgaaa atggtgatac
     5821 tgttgtaatt attgaacata acctagatgt tatcaaaaca gcagactata ttatagactt
     5881 aggtcctgaa ggtggtagtg gcggtggtac tattgttgcg actggcacac ccgaagatat
     5941 tgctcagaca aagtcatcat atacaggaaa gtatttaaaa gaagtacttg aacgagataa
     6001 acaaaatact gaagataaat aagattaaaa gaagtgaagg atgttataaa tttatccttc
     6061 gcttcttttt attaatttag taatgaatag tagaaagaaa agatgcgtaa aaagaattat
     6121 gttaagatag ggtcaatcta gagtagttaa acataaatcg aactgggagt gggacagaaa
     6181 tgataaagaa tcactaatga tttattatgt agtggttctt tgtcattagc cacagctatt
     6241 gtgtacttaa aaataggaat gcatgagtgc aactcatgca taagaaatac taatttctaa
     6301 agaaaaagta tttctttatg ttggggcccc gccaacttgc attgtttgta gaatttcttt
     6361 tcgaaattct ttatgttggg gccccgccaa cttgcattgt ttgtagaatt tcttttcgaa
     6421 attctttatg ttggggcccc gccaactaat tccaatatat cattgtagag cttaggtcat
     6481 tgatttttgg ctcggacttt tatggcgata tgaaccatgt aaattaagca agcaataaat
     6541 taatgattga tattgacttg taaaataata acaataatga acaattaata tttattttag
     6601 cttttcaatg tagattggtg ttatattttt gatatgataa gaagagatgt aagagtaggg
     6661 ataaatacaa ttgaggtgaa cccatgttaa cgacagaaaa actagttgaa acattaaagt
     6721 tagatttaat cgctggtgaa gaaggactat cgaagccaat taaaaatgct gatatatcaa
     6781 gaccgggctt agagatggca ggttattttt cacattatgc gtcagataga atacaactat
     6841 taggaacaac ggaactatcg ttttacaatt tattaccaga taaggatcgc gcaggtcgta
     6901 tgcgtaaact atgcagacca gaaacgcctg caattattgt gacacgtgga ttgcagccac
     6961 cagaagaatt agttgaagct gcaaaagaat taaatacccc acttatagtt gctaaagatg
     7021 cgactacaag tttaatgagt cgcttaacaa cgtttttaga gcatgcactt gcaaagacga
     7081 catctttaca tggtgtttta gtagatgttt acggtgttgg tgtactaatt accggtgatt
     7141 caggaatagg taaaagtgag actgcgttgg aattagttaa acgtgggcat agattagtag
     7201 cagatgataa tgtagaaata cgtcaaatta ataaagatga actaataggg aaaccaccaa
     7261 agttaataga acatctatta gaaatacgtg gactaggtat tatcaatgtt atgactttat
     7321 ttggcgcggg ttcaatatta actgaaaaac gaattagatt aaatattaat ttggaaaact
     7381 ggaacaagca aaagttatat gaccgcgtag gtcttaatga agagacgcta agtattttag
     7441 atactgaaat cactaaaaaa acaatacctg taagacctgg tagaaatgtt gcggtaatta
     7501 ttgaggtcgc tgcaatgaac tatcgattaa atatcatggg cattaacacg gccgaagaat
     7561 ttagtgaaag attaaatgaa gaaattatca agaacagtca taagagtgag gagtaggttg
     7621 aatgggtatt gtatttaact atatagatcc tgtggcattt aacttaggac cactgagtgt
     7681 acgatggtat ggaattatca ttgctgtcgg aatattactt ggttactttg ttgcacaacg
     7741 tgcactagtt aaagcaggat tacataaaga tactttagta gatattattt tttatagtgc
     7801 actatttgga tttatcgcgg cacgaatcta ttttgtgatt ttccaatggc catattacgc
     7861 ggaaaatcca agtgaaatta ttaaaatatg gcatggtgga atagcaatac atggtggttt
     7921 aataggtggc tttattgctg gtgttattgt atgtaaagtg aaaaatttaa acccatttca
     7981 aattggtgat atcgttgcgc caagtataat tttagcgcaa ggaattggac gctggggtaa
     8041 ctttatgaat cacgaggcac atggtggatc ggtgtcacgc gcttttttag aacaattaca
     8101 tttgcctaat tttataatag aaaatatgta t
//
LOCUS       DANJFZ010000117         8078 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.117, whole genome shotgun sequence.
ACCESSION   DANJFZ010000117 DANJFZ010000000
VERSION     DANJFZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.117"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            127..942
                     /locus_tag="PWN45_002287"
     CDS             127..942
                     /locus_tag="PWN45_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HDL0624032.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="PWN45_002288"
     CDS             1264..2442
                     /locus_tag="PWN45_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0624033.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="PWN45_002289"
     CDS             2702..3394
                     /locus_tag="PWN45_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0624034.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="PWN45_002290"
     CDS             3391..4944
                     /locus_tag="PWN45_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0624035.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="PWN45_002291"
     CDS             4947..5903
                     /locus_tag="PWN45_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624036.1"
                     /translation="MLKRTIKFILYLILSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="PWN45_002292"
     CDS             5903..6670
                     /locus_tag="PWN45_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624037.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="PWN45_002293"
     CDS             6637..7404
                     /locus_tag="PWN45_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624038.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="PWN45_002294"
     CDS             7397..8032
                     /locus_tag="PWN45_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0624039.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         2843 a         1247 c         1539 g         2449 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaacggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcttaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcccaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aagatcacaa
     8041 atcttaatat ggtgaatatt taatggtacc taaaaaat
//
LOCUS       DANJFZ010000118         7959 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.118, whole genome shotgun sequence.
ACCESSION   DANJFZ010000118 DANJFZ010000000
VERSION     DANJFZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.118"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            73..501
                     /locus_tag="PWN45_002295"
     CDS             73..501
                     /locus_tag="PWN45_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624040.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(710..1216)
                     /locus_tag="PWN45_002296"
     CDS             complement(710..1216)
                     /locus_tag="PWN45_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624041.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            1329..2252
                     /gene="graX"
                     /locus_tag="PWN45_002297"
                     /gene_synonym="apsX"
     CDS             1329..2252
                     /gene="graX"
                     /locus_tag="PWN45_002297"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDL0624042.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            2268..2942
                     /gene="graR"
                     /locus_tag="PWN45_002298"
                     /gene_synonym="apsR"
     CDS             2268..2942
                     /gene="graR"
                     /locus_tag="PWN45_002298"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDL0624043.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2935..3975
                     /gene="graS"
                     /locus_tag="PWN45_002299"
                     /gene_synonym="apsS"
     CDS             2935..3975
                     /gene="graS"
                     /locus_tag="PWN45_002299"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDL0624044.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            4119..4880
                     /gene="vraF"
                     /locus_tag="PWN45_002300"
     CDS             4119..4880
                     /gene="vraF"
                     /locus_tag="PWN45_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDL0624045.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            4870..6759
                     /gene="vraG"
                     /locus_tag="PWN45_002301"
     CDS             4870..6759
                     /gene="vraG"
                     /locus_tag="PWN45_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDL0624046.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            7484..>7959
                     /locus_tag="PWN45_002302"
     CDS             7484..>7959
                     /locus_tag="PWN45_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDL0624047.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQS"
BASE COUNT         2951 a          971 c         1390 g         2647 t
ORIGIN      
        1 gaaaagaatt caaaagaaaa attaaaatat agattgagca cgaagtgatt tgaaataagg
       61 ttgtgaaagg gaatgacaag gtcagcatta aaaccattta aaaataaacg cgttatggtt
      121 actggacgta tacaacgtgt tttgtttaaa aattatttag atagacatag cacatttaag
      181 ccgaatgtaa ggatattatt aaaagatgta tttgtttcag gtgtatcaat agatcattta
      241 tggttatatg agacaaataa atactatgca ttggcaatgg aacttattca tcaacgagta
      301 aaatttagtg cgaatgttgt accgtattac aaaataaata gaaataataa tttattcgta
      361 caagattatg gaattaagcg taaaggtagg ttaattactg aagaagctta caatcaaaac
      421 aatcagtatc aggataagat atatgaaaaa ttaccggata tagattttag actcgaagat
      481 ttttatagta aggaaaacta aacaacatat ataaagcacc actaatcgta gttgatagtt
      541 aatcaattac acattagtgg tgttttattt tgaatttaaa tttgataaat gaataagagt
      601 gtacgtattt atttgagatt agataacatt gataagtatg agtaatgtat taggtaacca
      661 ttaaattcgt tgttatacga ttctgataca agattatgat aaaatagctt taattaagaa
      721 ttttagccat catatagtca tcataatatt taccatcgat aaataactta tcttttaaaa
      781 cgccttcgat ttgaaaatcg gcacttttaa aaagctcgag ggcaggttgg ttattgagtg
      841 gtacatttgc ttcaattcgg tgtatttgat tgtttaaaca ccaagccata atggcatcaa
      901 gaagtgcttg gccaattcca cgatgttgat ataatttctt tacacctaaa tcaattttag
      961 caacatgttt aatgcgttga aatggtgtcg tattaacaaa ggcaaagcca acgagttgtt
     1021 catcactttc agcaacgaag atgactttat gcggagaagt gatatattct tctaattgtt
     1081 tactagccga tgtgacgcta ggatcatatt ctcctggtgt gtagaacata tacggagatt
     1141 cgtcgtatat gttcgctaac attgaaatga aattttctac atctttgata ctaactctac
     1201 gtataatatg ggccataaaa agcctccagt attttgaaat tttaaaacat ttgctactat
     1261 tataatatat atgtaactaa aaggtggagt aatatgtttt ttggttacag tttaaaggag
     1321 tattttagat gaaacctaaa gttttattag caggtggaac aggatatatt ggtaagtatt
     1381 taagtgaagt gattgaaaat gatgctgaac tttttgctat atcaaaatat ccagacaata
     1441 aaaaaacaga tgatgttgaa atgacttgga ttcagtgtga tatatttcat tacgaacagg
     1501 ttgttgcagc aatgaatcaa atagatattg ctgtattctt tatcgaccca acaaagaatt
     1561 ctgccaaaat aacacaatca tcagcaagag atttaacatt aatcgcagca gataattttg
     1621 gtcgagcagc ggctatcaat caagtaaaaa aagtaatcta catacctggg agtcgttatg
     1681 ataatgaaac aattgaacgc ctaggtgcat atggtacacc tgtagaaaca acaaatttag
     1741 tttttaaacg ttctttagtt aatgtagaat tacaagtttc aaagtatgat gatgttagat
     1801 caacgatgaa ggtagtttta ccaaagggat ggacattaaa gaacgttgta aaccatttta
     1861 ttgcatggat gggttacact aaaggaactt ttgtgaaaac agaaaaatca catgatcaat
     1921 ttaagatata tattaagaat aaggtgcgac cgctcgcagt atttaaaata gaagaaacag
     1981 ctgatggaat aataacttta attttattga gtggaagttt agtgaaaaaa tatacagtta
     2041 atcaagggaa gttagaattt agattaatca aagagtcggc agtcgtttat atacatctat
     2101 acgattatat ccctcgatta ttttggccga tttattactt tatacaagca ccaatgcaaa
     2161 aaatgatgat tcatggcttt gaagttgact gccggattaa agattttcaa agtcgattaa
     2221 aatcaggaga aaatatgaaa tatactaaat gatattgggt gatatggatg caaatactac
     2281 tagtagaaga tgacaatact ttgtttcaag aattgaaaaa agaattagaa caatgggatt
     2341 ttaatgttgc tggtattgaa gatttcggca aagtaatgga tacatttgaa agttttaatc
     2401 ctgaaattgt tatattggat gttcaattac ctaaatatga tgggttttat tggtgcagaa
     2461 aaatgagaga agtttccaac gtaccaatat tatttttatc atctcgtgat aatccaatgg
     2521 atcaagtgat gagtatggaa cttggcgcag atgattatat gcaaaaaccg ttctatacca
     2581 atgtattaat tgctaaatta caagcgattt atcgtcgtgt ctatgagttt acagctgaag
     2641 aaaaacgtac attgacttgg caagatgctg tcgttgatct atcaaaagat agtatacaaa
     2701 aaggtgatca gacgattttc ctgtccaaaa cagaaatgat tatattagaa attcttatta
     2761 ccaaaaaaaa tcaaatcgtt tcgagagata caattatcac tgcattatgg gatgatgaag
     2821 catttgttag tgataatacg ttaacagtaa atgtgaatcg tttacgaaaa aaattatctg
     2881 aaattagtat ggatagtgca atcgaaacaa aagtaggaaa aggatatatg gctcatgaat
     2941 aatttgaaat gggtagctta ttttttgaaa tctcgcatga actggatatt ttggatattg
     3001 tttttaaact tccttatgtt aggcattagt ctaatcgatt atgattttcc aatagacagt
     3061 ttattttata ttgtttcttt gaatttaagt ttaacaatga tttttctttt attgacatat
     3121 tttaaagaag taaaattata taagcatttt gacaaagata aagaaataga agaaattaaa
     3181 cataaagatt tagcggaaac gccatttcaa cgtcatacag ttgattattt atatcgtcaa
     3241 atctcagcgc acaaagaaaa ggttgttgag caacagttgc aattgaacat gcatgaacaa
     3301 accattacag aatttgtgca cgacataaaa acacctgtga cagctatgaa attattaatt
     3361 gatcaagaaa aaaatcaaga aagaaaacaa gcattactat atgaatggtc tcgtataaac
     3421 tcgatgctag atacacagct gtatattact agattagaat ctcaacgtaa agatatgtat
     3481 tttgattacg tgtcacttaa acgcatggtc attgatgaaa tacaattaac aagacatatt
     3541 agtcaggtta aaggtattgg ttttgatgtt gactttaaag tggatgatta tgtttataca
     3601 gatataaaat ggtgtcgtat gattattaga caaattttgt caaacgcatt gaaatatagt
     3661 gagaatttta atattgaaat tgggacagaa ttaaatgatc aacatgtttc gttatatatt
     3721 aaagactatg gcagaggtat tagtaaaaaa gatatgccgc gaatatttga acgaggattt
     3781 acgtcaacgg ctaacagaaa tgaaacgacg tcttcaggta tgggtctata tttagtaaat
     3841 agtgtaaagg atcaattagg tattcacctg caagtcacgt cgactgttgg taaggggaca
     3901 actgtcagat tgattttccc attacaaaat gaaattgttg aacgcatgtc ggaagtgaca
     3961 aatttgtcat tttaaacatg cgttttgtta cttagaattg atacatcaat gcagcttcaa
     4021 cgttataata agatagatgt tagtcatatg ttaaattgaa gatacaagtg ccaaagccta
     4081 aaggaaatga agttaagata aattatagga gtgttaaagt ggcaatttta gaagtaaaac
     4141 aattaacaaa aatatatgga actaaaaaaa tggcacaaga agtgttgcga gatatcaata
     4201 tgtctattga agaaggcgag tttattgcta ttatgggtcc ctctggatct gggaaaacga
     4261 cattattaaa tgttttaagt tcaattgatt atatttcaca aggttctatt acattaaaag
     4321 gaaaaaaatt agaaaagctt tcaaacaagg aattatctga tatacgcaag catgatattg
     4381 gttttatttt tcaagagtat aatttactgc atacattgac tgttaaagaa aacataatgt
     4441 taccactaac ggtacagaag ttagataaag aacatatgtt aaatcgttat gaaaaagtag
     4501 cagaagcatt aaatatattg gatattagtg ataaatatcc ctctgaattg tctggtggac
     4561 aaaggcaacg aacatcagct gccagagcat ttataacatt gccttctatt atatttgctg
     4621 acgaaccaac aggtgcattg gattctaaaa gtactcaaga tttattaaaa cgattaacaa
     4681 gaatgaatga agcatttaag tctacaatta ttatggtaac gcatgatcct gttgcagcaa
     4741 gctatgcaaa tcgagtagtg atgctaaaag atggtcaaat tttcactgaa ttataccaag
     4801 gggatgacga taaacatacc tttttcaaag aaataatacg tgtacaaagt gttttaggtg
     4861 gcgttaatta tgacctttaa cgagataata tttaaaaatt tccgtcaaaa tttatcacat
     4921 tatgccatct atcttttttc gttaattacg agtgtagtat tgtattttag ctttgtagca
     4981 ttaaaatacg ctcataaact aaacatgaca gagtcatatc caattataaa ggaaggctca
     5041 caagtcggaa gctactttct atttttcatc ataattgcat ttttgttata tgccaatgtg
     5101 ttatttatta aacgacgaag ttatgagctt gcattatatc aaacattagg tttatctaaa
     5161 ttcaacatta tttatatact aatgctcgaa caattactaa tatttataat tacggcaata
     5221 ttaggtatta ttattggtat ttttggttcg aaactgttat taatgattgt ctttacatta
     5281 ttaggaatta aagaaaaggt tccaattatt tttagtttga gggcggtatt tgaaacatta
     5341 atgttaatcg gtgtcgctta ttttttaaca tctgctcaaa attttatatt agtgttcaaa
     5401 caatctattt cacagatgtc aaagaataac caggttaaag aaacaaatca taataaaatt
     5461 acatttgaag aggttgtttt aggcatctta ggtatagtat tgattaccac aggatactat
     5521 ctatctttga acattgttca atattatgat tctatcggta cacttatgtt tattttattg
     5581 tcaactgtga ttggggcata cttatttttt aaaagctctg tttctctagt ttttaaaatg
     5641 gtgaagaagt ttagaaaagg tgttataagt gtaaatgatg tcatgttctc atcatctatt
     5701 atgtatcgta ttaagaaaaa tgctttttca cttacggtca tggcaatcat ttcagcgatt
     5761 actgtttcag ttctttgctt tgctgctata agtagagcgt ccttatcaag tgaaataaaa
     5821 tatactgcac cacacgacgt tacaattaaa gaccaacaaa aagctaatca attagcaagt
     5881 gaattaaaca atcaaaaaat tcctcatttt tataattata aagaagtaat tcatacgaaa
     5941 ttgtataaag ataatttatt tgatgtaaaa gcgaaagaac catacaatgt aacaattact
     6001 agtgataaat acatccctaa tactgatttg aaacgtgggc aagctgattt atttgtagcg
     6061 gaaggttcta tcaaagattt agtgaaacat aagaagcatg gtaaggcaat tataggaacg
     6121 aaaaaacatc atgttaatat taagttacgt aaagatatta ataaaatcta ttttatgaca
     6181 gatgttgatt taggtggacc aacgtttgtc ttaaatgaca aagactatca agaaataaga
     6241 aagtatacaa aggcaaagca tatcgtctct caatttggat tcgatttgaa acataaaaaa
     6301 gatgctttag cattagaaaa agcgaaaaat aaagttgata aatctattga aacaagaagt
     6361 gaagcgataa gctcaatatc aagtttaacc ggaatattat tatttgtaac atcattttta
     6421 ggtattacat tcttgattgc tgtatgttgc attatataca taaagcaaat agatgaaacc
     6481 gaagatgagt tagagaatta tagtattttg agaaagcttg gatttacaca aaaagatatg
     6541 gcaaggggac taaagtttaa aattatgttt aattttgggt tacctttagt tattgcacta
     6601 tcacatgcat attttacatc attagcatat atgaaattaa tgggtacaac gaatcaaata
     6661 ccggttttca tagtaatggg attatacatt tgtatgtatg ctgtttttgc agtgacggct
     6721 tataatcatt ccaagcgaac aattagacat tccatataaa atatacagat ggctttcagt
     6781 agagtagtgg attcggattc acgaactata ctggaagctt tttattataa atgaagagaa
     6841 gttatatttt tagcatgtat agttgaatac tgggttaaaa taccatatta ataatgaagt
     6901 aaaggtatga gtgattatga aagtgttttg aatgaaatat atttaattgg tgatgctttt
     6961 aattgaaaag attaacagga ttcaactttg taaattgtat taaatgtgag aaaataaaag
     7021 tatattcatt gagagatata tgagtcaatg atcgttttaa acaagataag tgtattttaa
     7081 tatgtaaaag ttatgtaata aatattgtat cgttgcaaat ttcccaacta tatccattta
     7141 caatttttag agttgtatag ataaaaaatt gcctatacaa taactttttc aaaacaaatg
     7201 cctactacta tttaaatttt atgtaaaagt taagtttcgg aattgttaaa aatttgtaaa
     7261 ttgaattgtg ggattgtaaa ttttgtgcta ttttcatata agggcaaata cagtaaattg
     7321 tttactgtga ggcatttttg aaattaatat cagtacacta aaattatact gacgattgat
     7381 aataacaaat ttgtatcatt agtttgtaaa ttcacttgtc ttaatttgaa acaaattata
     7441 actgaatgtg attggtgaca atcgcttaaa tggaggattt taaatgttta gtaagaaaaa
     7501 agataagttt atggttcaat tagaagagat ggttttcaat ctggatcgtg ctgctattga
     7561 attcggtaaa atggatttca atacacattt agatttaaaa gcatactcag acaacattaa
     7621 aacttatgag tcacatggtg acgaattagt acatcaagta attactgatt taaatcaaac
     7681 atttatcaca ccaattgaac gtgaagatat tttatcatta tgtgatgcaa ttgatgatgt
     7741 tttagatgca attgaagaaa cggcagctat gtttgaaatg tattcaatcg aatacacaga
     7801 tgaatatatg gctgagtttg ttgataacat tcaaaaagca gttgcagaaa tgaaacttgc
     7861 tgtcggctta ttagtcgata aaaaattatc acatatgcgt attcattcaa ttaatattaa
     7921 agaatttgaa acaaactgtg atggtatttt aagacagtc
//
LOCUS       DANJFZ010000119         7889 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.119, whole genome shotgun sequence.
ACCESSION   DANJFZ010000119 DANJFZ010000000
VERSION     DANJFZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.119"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            107..1282
                     /gene="rpsA"
                     /locus_tag="PWN45_002303"
     CDS             107..1282
                     /gene="rpsA"
                     /locus_tag="PWN45_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL0624048.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1504..2814
                     /gene="der"
                     /locus_tag="PWN45_002304"
     CDS             1504..2814
                     /gene="der"
                     /locus_tag="PWN45_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL0624049.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2831..3829
                     /locus_tag="PWN45_002305"
     CDS             2831..3829
                     /locus_tag="PWN45_002305"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0624050.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4000..4272
                     /locus_tag="PWN45_002306"
     CDS             4000..4272
                     /locus_tag="PWN45_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL0624051.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            4703..5275
                     /locus_tag="PWN45_002307"
     CDS             4703..5275
                     /locus_tag="PWN45_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDL0624052.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            5278..6003
                     /locus_tag="PWN45_002308"
     CDS             5278..6003
                     /locus_tag="PWN45_002308"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDL0624053.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            6005..6964
                     /locus_tag="PWN45_002309"
     CDS             6005..6964
                     /locus_tag="PWN45_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0624054.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            7056..7505
                     /gene="ndk"
                     /locus_tag="PWN45_002310"
     CDS             7056..7505
                     /gene="ndk"
                     /locus_tag="PWN45_002310"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL0624055.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            7714..>7889
                     /locus_tag="PWN45_002311"
     CDS             7714..>7889
                     /locus_tag="PWN45_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624056.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVLVKELIIFL"
BASE COUNT         2874 a         1034 c         1522 g         2459 t
ORIGIN      
        1 ttatgtatat gactatttcg caaaatgtaa tcgaggtaga atttcttgac aattctgtca
       61 gtttataaga tgttataaat atgtagtgta taaggaggca aacaagatga ctgaagaatt
      121 caatgaatca atgattaacg atattaaaga aggtgacaaa gtcactggcg aggtacaaca
      181 agttgaagac aagcaagttg ttgttcatat caacggtggt aaatttaatg ggattattcc
      241 tattagtcaa ttatctacgc atcatattga tagcccaagt gaagttgtaa aagagggcga
      301 cgaagttgaa gcatatgtca ctaaagttga gtttgatgaa gaaaatgaaa ctggagctta
      361 catcttatct agaagacaac ttgaaactga gaagtcttat agttatttac aagaaaaatt
      421 agataataat gaaatcatcg aagcgaaagt aacagaagta gttaaaggtg gtttggttgt
      481 tgatgtagga caaagaggtt ttgttccggc ttcactaatt tcaacagact tcattgagga
      541 tttctctgtg tttgatggac aaacaattcg tattaaagtt gaagaattgg atcctgaaaa
      601 taatagagtc attttaagcc gtaaagcagt tgaacaagaa gaaaacgatg ctaaaaaaga
      661 tcaattatta caatctttaa atgaaggcga tgttattgat ggtaaagtag cgcgtttaac
      721 tcaatttggt gcatttatag acattggcgg tgttgatggt ttagtgcatg tatctgaact
      781 ttctcacgaa catgttcaaa caccagaaga agtagtttca attggtcaag atgttaaagt
      841 taaaattaaa tctattgata gagatacaga acgtatttca ttatcaatca aagatacgtt
      901 accaacacct ttcgaaaata ttaaaggtca attccacgaa aatgatgtca ttgaaggtgt
      961 cgtagtaaga ttggcaaact ttggtgcatt tgttgaaatt gcaccaggtg tacaaggact
     1021 tgtacatatt tctgaaattg cacacaaaca cattggtacg ccaggtgaag tgttagaacc
     1081 tggtcaacaa gtaaatgtta aaatattagg tattgatgaa gagaatgaaa gagtatcact
     1141 atctattaaa gcaacattac caaacgaaga tgttgttgaa agtgatcctt ctacgactaa
     1201 ggcgtactta gaaaacgaag aagaagataa tccaacaatt ggcgatatga ttggtgataa
     1261 acttaaaaat cttaaactat aatttaatat ttaatagtca actccacatg tttatgattg
     1321 catgtggagt atttttatgt aacaaaatat actcggaatg ataacgtgga caaatttaac
     1381 taagtgttta aaaagataaa gttttaagtg ctgattttta tcattacagt aataaactca
     1441 ttttgaatac acagtctcat gtgatattat taaaaagata taagaaagag aggaagttag
     1501 cttatgacta aacctatagt agctattgta ggtaggccta atgtaggtaa atctacaatt
     1561 tttaatagaa tagttggaga acgtgtttcg attgtggaag acacgccagg tgtaacacga
     1621 gatcgtattt attcttcagg tgaatggtta acacatgatt tcaatattat tgatacaggt
     1681 ggtattgaaa ttggtgatgc accattccaa acacaaatta gagcgcaggc agaaatcgcc
     1741 atagatgaag cgtatgttat tatttttatg gttaacgtgc gtgaaggatt gacacaaagc
     1801 gatgaaatgg tcgctcaaat tttatacaaa tctaaaaaac cggtcgtatt agcggttaac
     1861 aaagtagata atatggaaat gcgtacagac gtgtatgatt tctattcatt aggatttggt
     1921 gaaccgtatc cgatatcagg gtcacatggt ttaggtcttg gtgacttgtt agatgcagtt
     1981 gtttctcatt ttggtgaaga ggaagaagat ccttatgatg aagatacaat tcgactatcc
     2041 attattggac gaccaaacgt aggtaaatca agtttagtaa atgctatttt aggtgaagat
     2101 cgcgttatcg tttctaatgt tgcagggaca acgagagacg ctattgatac agagtatagt
     2161 tatgatggac aagattatgt tttaatcgat actgctggta tgcgtaaaaa aggaaaagta
     2221 tatgaatcaa ctgagaaata ttcagtatta agagctttaa aagcgattga acgttcaaat
     2281 gttgttttag tggtcataga tgcagaacaa ggcatcattg aacaagataa acgtgttgca
     2341 ggatatgcac atgaacaagg taaagcagtc gtgattgtcg taaataaatg ggatactgtg
     2401 gaaaaagata gtaaaacgat gaagaaattt gaagatgaag tacgtaaaga attccaattt
     2461 ttagattatg cacaaattgc ttttgtgtct gctaaagaac gcacaagatt acgtacatta
     2521 ttcccttaca tcaatgaagc aagtgaaaac cataaaaaac gtgttcaaag ttcaacttta
     2581 aatgaagttg ttactggtgc aatttccatg aaccctacac caacagacaa aggtagacgt
     2641 ttgaatgtct tttatgcaac acaagttgct atagaaccac cgacatttgt tgtatttgtt
     2701 aatgatgtag aattaatgca tttttcttat aaacgctatt tagagaatca aatccgtgcc
     2761 gcttttggtt ttgaaggtac accaattcat attatagctc gaaagagaaa ttaacgattg
     2821 ggggataaca atgactaaaa ttaccgtttt tggtatggga agttttggga cagcccttgc
     2881 caatgttctt gcagaaaatg gacatgatgt tttgatgtgg ggtaaaaatc aagatgctgt
     2941 tgatgaatta aatacatgtc atacaaataa aaagtattta aaatacgcga aattagatgt
     3001 taacatcatc gctacttcag atatgaccaa agcaattcaa tttgcagata tttacttaat
     3061 ggctttacct actaaagcaa tgcgagaagt tgcttctcaa attaatgata agctgacctc
     3121 taaaaagact tttatacatg ttgctaaagg tattgaaaat gggacgttta aacgtgtgtc
     3181 agaaatgatt gaagattcta tttcacctga atataatgca ggtattggcg tgttgtcagg
     3241 gccaagtcat gcggaagaag ttgtagtcaa gcaaccaact acagttgctg cttcatcaaa
     3301 agataaaagt gtaagtaaat taacgcaaga tttatttatg aatgattatt tgcgtgtgta
     3361 cacgaatgat gacttgattg gtgttgaact tggtggtgca ttgaaaaata tcatcgcagt
     3421 agcaagtggt atcgtagctg gaattggcta cggtgataat gcaaaagctg cattaatgac
     3481 tcgtggctta gcggaaatta gtagattagg tgaaaagtta ggtgccgatc ctatgacatt
     3541 tctaggttta ggtggtatcg gtgacttaat cgttacttgc acatcaacac attctcggaa
     3601 tttcacatta ggatataaac ttggacaagg tgaatcaatg gatcaagcat tatctgaaat
     3661 gaatatggtt gttgaaggta tttatacaac taaatcagtt tatcatttag ctaaagaaaa
     3721 aaatgtggat atgccaatta caaatgcatt atatagagta ttatttgaaa atatctcagt
     3781 aaaagaatgc gtaaaagatt taatggagcg cgataaaaaa tctgaataaa atgtgtatta
     3841 ttacacatat acggctaata attacgtaaa atgaatagaa atggcttaaa ttcaacgttt
     3901 ttgccacata aatgattgcg atttatgtaa tgcttgtgtt aaagttttac cataatgata
     3961 taaacatatc attatcaaac ctcaggaggt gaatgtctaa tgaacaaaac agatttaatc
     4021 aatgcagttg cagagcaagc tgatttaact aaaaaagaag ctggttcagc agtagatgct
     4081 gtattcgaat caatccaaaa ctcacttgct aaaggtgaaa aagtacaatt aattggtttc
     4141 ggtaactttg aggtacgtga acgtgctgca cgtaaaggtc gtaaccctca aactggtaaa
     4201 gaaattgata tcccagcaag taaagttcca gcattcaaag ctggtaaagc attaaaagat
     4261 gctgtaaaat aattttactt aaaaagccct gaataagggc tttttatttt gcttttaata
     4321 cttacaacct gtacataaat tgtaatgttc ttctaagttt ttaatctttg gaacattcat
     4381 tgtttaagcg ttcaaaatat tacatgacat aatagttttg ataaaaatag caactacaaa
     4441 atatacatat gaatattgag attaattgtt agcgttgaat ttacttaaaa ggtaaccatg
     4501 tctactatag tattttacgt tatttaaaaa gatgaataat gtaaatgaag taaaggttat
     4561 tatgagaatt acaaaagcta cataaattac tgttagttta aattgaaatt taaaaatgat
     4621 aagtaatctt aatgcttcaa aagattttaa tttcttacat ataatgttaa tatgaatagt
     4681 gataatatcg tgaggtgtag acatggaaac aactgttagc aaattggaaa gacaaataga
     4741 agaaagatta aagggcgtat cagaatatga atccattaat attaatcatc ggttaggtaa
     4801 gttacttgat ttatatgata ttccagatgt tgccaaagtg gcatgcctca caattgacac
     4861 atcaatgaga caccttgatg acattacata taatcatttg tctaaacatt ccattttgat
     4921 tggagattta attagtgcac atttttatac gctactagca gaaatcaatg atttatcatt
     4981 tcaaaatgaa attagtaaag caattgttga aattaatgaa ttgaaatctt cattacatca
     5041 tcaagcttta aatgattatg aaatttctca agcaatagta aaaattgaaa cacttttccc
     5101 atatattacg ttatctcatt tcggtatcaa tattgatgag tcggaaatat ataattattt
     5161 atttgaagat atgtctgatt actatccatc atacttcaag aaatataatc aaagtgaagt
     5221 aaagcattac ttacacgata tccaaaaaag ttatttaaaa agtagaggta attaagcatg
     5281 gctgacaata aagcaaataa agagcaagta catcgcgttt ttcaaaatat ttcaaaaaaa
     5341 tatgatagat taaataatat tattagtttt gagcagcata aagtatggag aaaacgcgtc
     5401 atgaaagaca tgggtgttag aaaagggacg aaagcattag atgtttgttg tggtactggt
     5461 gattggacaa tcgcattaag caaagctgta ggaccaacag gtgaagttac tggtattgac
     5521 tttagtgaga atatgttaga agtaggaaaa gaaaaaactg cttcaatgga aaatgttaaa
     5581 cttgttcatg gtgatgcaat ggaattgccg tttgaagaca attcttttga ttatgtaaca
     5641 attgggtttg gattaagaaa tgtgccagac tatttagtcg cgttaaaaga aatgaataga
     5701 gtacttaaac caggtggtat ggtggtatgt cttgaaacga gccaaccaac tttgccagtc
     5761 tttaaacaaa tgtatgcact atactttaaa tttgttatgc ctatttttgg aaaattattt
     5821 gcaaaatcaa aagaagaata tgaatggtta cagcaatcta cgtttaattt tcctggaaaa
     5881 gaagagttga agcgcatgtt tgaagaagca ggtttcataa atgtaagagt acgtagtttt
     5941 acagggggcg ttgctgcaat gcaccttggc tataaagaaa aagataatac caaaggtgat
     6001 taacgtggca aagttaaaca tgaacaatga aattaagaaa gtggaacaac gacttgaaaa
     6061 ggcaataaaa agtaaagatt ctgtattaga acaggcatca ttacacttat tgtcttctgg
     6121 tggtaaaaga gtacgcccag catttgttat tctgagtagc caatttggca aagatgaaca
     6181 aacgtctgaa caaacgtatc aagttgcagt cgcattagag ttaattcata tggcaacact
     6241 tgttcatgat gacgttattg ataaaagcga caagcgtcga ggcaagttaa ccatatcaaa
     6301 gaaatgggat cagacaactg ctattttaac tgggaatttt ttattggcat taggacttga
     6361 acacttaatg gccgttaaag ataatcgtgt acatcaattg atatctgaat ctatcgttga
     6421 tgtttgtaga ggggaacttt tccaatttca agaccaattt aacagtcaac agacaattat
     6481 taattattta cgacgtatca atcgcaaaac agcactgtta attcaaatat caactgaagt
     6541 tggtgcaatt acttctcaat ctgataaaga gactgtacga aaattgaaaa tgattggtca
     6601 ttatataggt atgagcttcc aaatcattga tgatgtatta gacttcacaa gtaccgaaaa
     6661 gaaattaggt aagccggtcg gaagtgattt gcttaatggt catattacgt taccgatttt
     6721 attagaaatg cgtaaaaatc cagacttcaa attgaaaatc gaacagttac gtcgtgatag
     6781 tgaacgcaaa gaatttgaag aatgtatcca aatcattaga aaatctgaca gcatcgatga
     6841 ggctaaggca gtaagttcga agtatttaag taaagctttg aatttgattt ctgagttacc
     6901 agatggacat ccgaaatcac tacttttaag tttgacgaaa aaaatgggtt caagaaacac
     6961 gtagtattta tgaaaagtat tgaaagcgct ttaccaacct gttaatatat aatagtaata
     7021 tacataaacc tattaaacac agggggatac ataaagtgga acgtacattt ttaatgatta
     7081 aaccagatgc agtacaaaga aatctaattg gtgaagtaat ttcaagaatt gaaagaaaag
     7141 gactaaaact tgtcggtggt aaattaatgc aagtaccaat ggaacttgct gaaacacatt
     7201 atggtgaaca ccaaggtaaa ccattttata atgatttaat ttcatttatt acatcagcac
     7261 cagtgttcgc aatggtagtt gaaggtgaag atgcagttaa tgtatctaga catattattg
     7321 gcagcaccaa tccttcagaa gcttcaccag gatcaattag aggtgattta ggtttaactg
     7381 ttggtagaaa tatcattcac ggttcagatt cattagagtc tgctgaacgt gaaattaatc
     7441 tatggtttaa tgaaaatgaa attactagct atgcttcacc acgtgatgca tggttatatg
     7501 aataaaatat aaactgtaaa cctttacgat ttatttataa aggtagaaag ggttttgtta
     7561 tgtggttagt cattatgatt atacataaca aggcccgttt tttatgttgt agtaaattac
     7621 ttgaaaaatt ttatagtttt ttggtaacac gtattaaaaa gagaggaata ttctttatca
     7681 aatgaaacta aacagagaga aggggttgtt aaaatgaaga atattatttc gattattttg
     7741 gggattttaa tgttcttaaa attaatggaa ttactatatg gtgctatatt tttagataaa
     7801 ccacttaatc ctataacaaa aattattttt atactgactc tcatttatat tttttatgta
     7861 ttagtaaaag aattgattat atttttgaa
//
LOCUS       DANJFZ010000120         7541 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.120, whole genome shotgun sequence.
ACCESSION   DANJFZ010000120 DANJFZ010000000
VERSION     DANJFZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.120"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            108..308
                     /locus_tag="PWN45_002312"
     CDS             108..308
                     /locus_tag="PWN45_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624057.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            385..516
                     /locus_tag="PWN45_002313"
     CDS             385..516
                     /locus_tag="PWN45_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624058.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            778..1437
                     /gene="arlR"
                     /locus_tag="PWN45_002314"
     CDS             778..1437
                     /gene="arlR"
                     /locus_tag="PWN45_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDL0624059.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1434..2789
                     /gene="arlS"
                     /locus_tag="PWN45_002315"
     CDS             1434..2789
                     /gene="arlS"
                     /locus_tag="PWN45_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDL0624060.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            3073..5871
                     /locus_tag="PWN45_002316"
     CDS             3073..5871
                     /locus_tag="PWN45_002316"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL0624061.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5885..7153
                     /gene="sucB"
                     /locus_tag="PWN45_002317"
     CDS             5885..7153
                     /gene="sucB"
                     /locus_tag="PWN45_002317"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL0624062.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2850 a         1080 c         1356 g         2255 t
ORIGIN      
        1 aaacaaaagc agtaaaccta aagtgtcgta agggtttact gcttttataa aacgttgtta
       61 taacgtatat tgtcttttac gggcatataa aaggggaata tttgaaaatg accaatccaa
      121 caagaacgaa acgttgtggg ggggatgttc tatatggtat tgataatcat tttcaactac
      181 tattatacat tagtgagaat cattgtcaat tagaaactaa aacttttttt gaatattttt
      241 taagaatagt aaataaaacg catgattacg ctattttaga aaataaaaaa atttgtattt
      301 ctcattagaa ttagaatatt taaaagtgat gaggtttaaa cattatattg tttacatact
      361 ccttttgaat tcatacatta tgaaatgtta cttccaagtt caaaatcgca cattgaaatg
      421 atgtgtgaaa tgtttaaact acggtcattt tgtgaaaata aagataataa ctattcattt
      481 tacaatagtg aaaagtcagt atatgacaac aattaatatt gcggtaaggc cttgtgttac
      541 agtattctat atttaagtac tgcaatcaga attaacagaa tgccattaac tgattattaa
      601 atatttgagt taataaataa ttaatgattg tagcttgaaa aatttaaaac atggttattg
      661 atttgtgata aaatttaaac gtaaacaaac taatttaaaa agcaactatt gtatagaaaa
      721 atacaaaatt taaaatatat taccttatta gaaaaagtcg taatatgagg tgtacaaatg
      781 acgcaaattt taatagtaga agatgaacaa aacttagcaa gatttcttga attggaactc
      841 acacatgaaa attacaatgt ggacacagag tatgatggac aagacggttt agataaagcg
      901 cttagccatt actatgattt aatcatatta gatttaatgt tgccgtcaat taatggctta
      961 gaaatttgtc gcaaaattag acaacaacaa tctacaccta tcattataat tacagcgaaa
     1021 agtgatacgt atgacaaagt tgctgggctt gattacggtg cagacgatta tatagttaag
     1081 ccgtttgata ttgaagaact tttagcaaga attcgtgcaa ttttacgtcg tcagccacaa
     1141 aaggatatta tcgatgtcaa cggtattaca attgataaga acgcttttaa agtgacggta
     1201 aatggcgcag aaattgaatt aacaaaaaca gagtatgatt tactatatct tctagctgaa
     1261 aataaaaacc atgttatgca acgggaacaa attttaaatc atgtatgggg ttataatagt
     1321 gaagtagaaa caaatgtcgt agatgtttat ataagatatt tacgaaacaa gttaaaacca
     1381 tacgatcgtg acaaaatgat tgaaacagtt cgtggcgttg ggtatgtgat acgatgacaa
     1441 aacgtaaatt gcgcaataac tggattattg ttaccacgat gattacgttt gtcacgatat
     1501 ttttgttttg tttaattatt atttttttct tgaaagatac actgcataat agtgagcttg
     1561 atgatgcaga acgaagctca agcgatatta ataatttatt tcattctaag cctgttaaag
     1621 atatatctgc attagacttg aatgcatctt taggtaattt tcaagagata attatttatg
     1681 atgagcataa taataaatta tttgagacat cgaatgataa cacagtgaga gttgaaccag
     1741 gttatgaaca ccgttatttt gaccgcgtaa taaaaaaacg ctataaaggc attgaatatt
     1801 taattattaa agaaccaatt acaacgcaag atttcaaagg gtatagcttg ttaattcatt
     1861 cactagaaaa ttatgataac atcgtaaaat cattgtatat cattgcgctg gcatttggag
     1921 tgattgcaac aattataact gccacaatca gttatgtatt ttcaacacaa attactaaac
     1981 cgcttgtcag tttatcaaat aaaatgattg agattcgacg agatggtttt caaaataaat
     2041 tgcaattaaa tacaaattat gaagaaatag ataatttagc aaatacgttt aatgagatga
     2101 tgagccaaat tgaagaatca tttaatcaac aaagacaatt tgttgaagat gcgtcacatg
     2161 aattacgaac accattacaa attattcaag gtcatttaaa tttgattcag cgatggggaa
     2221 aaaaagaccc agcagtatta gaagaatcgt taaatatttc tattgaagaa atgaatcgta
     2281 tcataaaatt agtcgaagaa ttacttgaat tgactaaagg agatgtaaat gacatttctt
     2341 ctgaagcaca gaccgtgcat attaatgatg aaattcgctc gcgaatacac tcattaaaac
     2401 aattgcatcc tgattatcaa tttgatacgg atctgacatc taaaaatcta gaaattaaaa
     2461 tgaaacctca tcaattcgaa caattatttt taatctttat tgataatgca atcaaatatg
     2521 atgtgaagaa taagaaaatt aaagttaaga caaggttaaa aaataagcaa aaaataattg
     2581 aaattacaga tcatggaatt ggtattccag aggaagatca agatttcatt tttgatcgct
     2641 tttatcgagt ggataaatct cgttcaagaa gtcaaggcgg taatggactc ggattatcta
     2701 ttgctcaaaa aatcattcaa ttaaacggag gatcgattaa aattaaaagt gaaattaaca
     2761 aaggaacaac gtttaaaatc atattttaat catgactgag acgtcaatca aagtcatagg
     2821 atcaattttt taagtacaca ttagctgtga ctaatgtata agaacaacta taaaacaaat
     2881 aaacagtggt tctttatcat ttctgttgta ctcccaaaat ttacaataaa atacatctat
     2941 aaacctagaa gaatcaacgc ttttgttgat tcttcttttt agcagataaa taggtaaatc
     3001 tactttaaca aataactaaa tagtgatatt attacattgt aagcgtttca acatttttgt
     3061 ggagggtgta aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt
     3121 gcgaatttag gtctaatgtt agatctatat gatgactttt tacaagatcc atcatctgta
     3181 ccagaagatt tacaagtctt attcagcaca attaagaatg atgactcaat tgtaccagct
     3241 ttaaaaagta caagtagtca aaatagcgac ggcacaatta agcgtgtcat gcgtttaatt
     3301 gataatattc gccaatacgg gcatcttaaa gccgatattt atcctgtaaa tcctccaaaa
     3361 aggaaacatg tacctaaatt agagattgaa gactttgatt tagatcaaca gactttggaa
     3421 ggtatatcag caggaattgt ttcagatcac tttgccgaca tttatgataa tgcttatgaa
     3481 gcaattttaa gaatggaaaa acgttacaaa ggaccaattg catttgagta tacacatatt
     3541 aataacaata ccgaacgtgg ttggttaaaa agaagaattg aaacgccata taaagtaacg
     3601 ttaaataata acgaaaaaag ggcactattc aaacaattag cgtatgttga agggtttgaa
     3661 aaatatcttc ataaaaactt cgttggtgca aagcgttttt caattgaagg ggtagacgca
     3721 cttgtaccga tgttacaacg tactattacg attgctgcga aagaaggtat taaaaatata
     3781 caaataggca tggctcaccg tggacgttta aacgttttaa cgcatgtctt agaaaaaccg
     3841 tacgaaatga tgatttcaga atttatgcat acagatccaa tgaaattctt acctgaagat
     3901 ggtagcttgc agttaactgc tggatggact ggtgatgtga aatatcacct tggtggcatt
     3961 aaaactactg attcatacgg tacaatgcag cgtattgcac tggctaacaa tccaagtcac
     4021 ttggaaattg ttgcacctgt tgttgagggg cgtacgagag cagcacaaga tgatacacaa
     4081 cgagctgggg ctccgacgac tgatcatcat aaagcaatgc caattattat acatggcgat
     4141 gctgcttatc ctggtcaagg aattaacttc gaaacaatga acttaggaaa cttgaaaggc
     4201 tattctacgg gtggttcatt gcatattatt actaacaata gaattggatt tactacagaa
     4261 ccaattgatg cacgttcaac aacttattct acagatgtgg ccaaaggtta tgatgtgcca
     4321 atattccatg tcaatgcaga tgacgttgaa gctactattg aagcaattga tattgcaatg
     4381 gaatttagaa aagagtttca taaagacgtc gttattgatt tagtaggtta tcgtcgtttc
     4441 ggacataacg aaatggatga accatcaatt actaatccag ttccttatca gaatattcgc
     4501 aaacatgact ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca
     4561 gaagatgaaa tgcattcatt tatagaacaa gtccaaaagg aactaagaca agctcatgat
     4621 aaaattaata aagctgataa aatggataat ccagatatgg aaaagcctgc agatcttgca
     4681 ttaccgttac aagcagacga acaatcattt acttttgatc acttgaaaga aataaatgat
     4741 gcattgttaa catatccgga tggctttaac attttgaaaa agttaaacaa agttcttgag
     4801 aagcgtcatg agccgtttaa taaagaagat ggtttagttg attgggcaca agcagaacaa
     4861 cttgcatttg cgacaatttt acaagatggt acaccgattc gcttaactgg tcaagatagt
     4921 gaacgtggta cattcagtca taggcatgcc gtgttacatg atgagcaaac aggtgaaaca
     4981 tatacacctt tacatcatgt tcctgatcaa aaagcgacat ttgatataca caattctccg
     5041 ctttcagaag cagcagtagt tggttttgaa tacggctata atgtggaaaa caaaaaaagc
     5101 ttcaatattt gggaagcaca atatggtgat tttgcaaata tgtcacaaat gatttttgac
     5161 aacttcttat tcagttctcg ctcaaaatgg ggagaacgtt caggattaac attattctta
     5221 cctcatgcat atgagggtca agggcctgaa cattcatcag caagattaga gcgattttta
     5281 caattagctg ctgaaaataa ttgcacagtt gtcaacttat ctagttcaag taattatttc
     5341 cacttattgc gtgcacaagc ggctagttta gattctgaac aaatgcgacc attggttgtt
     5401 atgtcaccaa aaagcttact gagaaataaa acagttgcaa aaccaattga tgaatttact
     5461 tctggtggat ttgagccaat tttgacagaa tcatatcaag cggataaggt tacaaaagtt
     5521 attttggcaa ctggtaaaat gttcattgat ttaaaagaag cattagctaa aaatccagac
     5581 gaatcagtat tactcgttgc gattgaaaga ttgtatccat tcccagagga agagattgaa
     5641 gcattactag cacaattgcc aaaccttgaa gaagtgtcat gggtacaaga agaacctaaa
     5701 aatcaaggtg catggttata tgtctatcca tatgttaaag tgctagttgc agataaatat
     5761 gatttaagtt atcatggcag aattcaaagg gctgctccag ctgaaggcga tggagaaatt
     5821 cataaacttg ttcaaaataa aattatagaa aatgcattaa aaaataacta gggggaaata
     5881 agtcatgcca gaggttaaag ttccagaatt agcagaatct attacagaag gtaccattgc
     5941 agaatggttg aaaaacgtag gggatagcgt agaaaaaggt gaagctattc ttgaattaga
     6001 aactgataaa gttaatgtcg aagttgtatc tgaagaagca ggtgtattat ctgaacaact
     6061 tgcaagtgaa ggcgacactg tagaagttgg acaagcaatt gctatcatcg gcgaaggtag
     6121 tggcaatgct tctaaagaaa atagtaacga caatactcca caacaaaatg aagaaacaaa
     6181 taataaaaaa gaagaaacaa caaataattc ggtagataaa gctgaagtaa atcaagcaaa
     6241 tgatgacaat cagcaacgta ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa
     6301 tggtgtgaat cttgctgaag taagtccgaa aacaaatgat gtggttcgta aagaagatat
     6361 tgataagaaa caacaggcac cggcatcaac acaaacaaca caacaagcat ctgcaaaaga
     6421 agagaaaaaa tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa tgtcacgtag
     6481 aaagaaaaca gctgccaaaa aattattaga ggtatctaat aatacagcta tgttaacaac
     6541 atttaacgaa gttgacatga caaatgttat ggaattgcgt aaacgtaaga aagaacaatt
     6601 tatgaaagat catgatggta ctaaattagg atttatgtca ttctttacta aagcttctgt
     6661 agcagctttg aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg acatgattac
     6721 gaaacaatat tatgatattg gtgtagctgt ttctacagat gatggattat tagtaccatt
     6781 tgtaagagat tgtgataaaa agaattttgc agaaatcgaa gcagaaattg ctaatttagc
     6841 agttaaagca cgagagaaaa aacttggctt agatgatatg gttaatggtt catttacgat
     6901 tacaaatggc ggtatttttg gatcaatgat gagtacgcca attatcaatg gtaatcaagc
     6961 tgcaatctta ggcatgcatt caattattac aagaccaatt gcgattgatc aagatacaat
     7021 cgaaaatcgt ccaatgatgt atattgcatt aagctatgat catagaatta ttgacggtaa
     7081 agaagcagtt ggattcttaa aaacaattaa agaattaatt gaaaacccag aagacttatt
     7141 attagaatct taatccacaa cacaaaatag tttactattc ctaaaagcgg gattaaatca
     7201 ataacaaaca gcagtaagat tatttcctag tcgaaatatc ttactgctgt actttatttt
     7261 tataatgatt cgtattaaaa tgtgggttta attttgacta cttaaaaaat atatcatttc
     7321 tattgaaata gactcacaat agaaatatag gtagaatagc gtgtttcaat atgctaatga
     7381 atgcatttta gataataaca aacgatgaaa tatatgaatt ctataagcgc ctttagttaa
     7441 tttaaaatca tctgaacatg atgtaaattc gctttatgga cacgacatat atatgttttt
     7501 catgtcctac aaaaaacgaa atattcaatt gctatataca g
//
LOCUS       DANJFZ010000121         7371 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.121, whole genome shotgun sequence.
ACCESSION   DANJFZ010000121 DANJFZ010000000
VERSION     DANJFZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.121"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            372..1433
                     /locus_tag="PWN45_002318"
     CDS             372..1433
                     /locus_tag="PWN45_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDL0624063.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1691..3148
                     /locus_tag="PWN45_002319"
     CDS             1691..3148
                     /locus_tag="PWN45_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0624064.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3141..3863
                     /locus_tag="PWN45_002320"
     CDS             3141..3863
                     /locus_tag="PWN45_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0624065.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            4014..5141
                     /gene="queG"
                     /locus_tag="PWN45_002321"
     CDS             4014..5141
                     /gene="queG"
                     /locus_tag="PWN45_002321"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL0624066.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5146..5616
                     /gene="trmL"
                     /locus_tag="PWN45_002322"
     CDS             5146..5616
                     /gene="trmL"
                     /locus_tag="PWN45_002322"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL0624067.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5775..6374
                     /locus_tag="PWN45_002323"
     CDS             5775..6374
                     /locus_tag="PWN45_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDL0624068.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6399..6551
                     /locus_tag="PWN45_002324"
     CDS             6399..6551
                     /locus_tag="PWN45_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624069.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2602 a          961 c         1364 g         2444 t
ORIGIN      
        1 tttaacttct ttttttggta ttacattaat gataatgatt atattatctg gggttatata
       61 tttgtttcaa cttttctaat aatatatttg taaaagtgct attttattgt gctttatacc
      121 aagtcgattc aatttatcta tcttaatagt aatatggcta ttttaatatt tacaatttat
      181 tacattatta gtcacaggat gtaattagta gtattttaaa attaacatga cattgattag
      241 tgagtagatt gtttaaattt gctttattca atttgtggat tgttatattt agattaaatt
      301 agccaactaa tcattgttaa agttaatttt ctataatatg atgagtttaa tgttcaaatt
      361 ggaggtcgtt tatgagtaac gcaaataatg ctgataataa acagtttttc agtaagattt
      421 taaattctat tggtgctggt gttgttattg cacttgttcc gaatgcttta ttaggtgaag
      481 tattaaaaat atttaaaagt ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac
      541 taattcaatc atttatggca tttattattg gggttttagc tgctcatcaa tttaaattta
      601 atggtgcagg tgctgctata gtaggtacat cagctatgat tggttcaggt gctgttgtat
      661 atagcaataa tagttttatg ttaaaaggta taggtgacat tataaatacg agtttggttg
      721 ttattattgc atgtcttata tatatggtat tacaaaacaa acttggatct tttgaattaa
      781 ttatattacc agttttagta cctatcgtta gtgggggtat cggtctaatc acattacctt
      841 acattcgaaa aattactcaa gcaattggaa atgtaattca ttcttttaca gacttaaatc
      901 cgttgttaat gtctatactt attagtgtgg cattttcatt attaatggta acaccaatat
      961 cgttagtagc gattgcaact gcgattagtt taaacggact aggaagtgga gcagcaaacc
     1021 taggtatagt ggctgcctgt gttactttct tatttggctc attgcgtgtc aattctattg
     1081 gtgtaaatgc agttttactc ataggagctg caaaaatgat gatcccagtt tacttgaaaa
     1141 atttaattat ttcgatacca ttgactataa atggaataat tactggaatc attgcttacg
     1201 ttcttcaggt taaaggaaca ccattgtctg ctggatttgg atatacagga ttagttggcc
     1261 caataaatgc atttaaccga atgtcaggtg atccaacaat gaatattatt ttattagcac
     1321 ttggttattt tgtcatacca tttgtatcgg catttattgt tcatgaattg tgtaaaaaat
     1381 tcattcctat atattctaat gatatttata aatttgaagt tccaaaacaa taatttatag
     1441 cttgatgaca agaataagtt ttaatatatg ataaatatag gcgttactgt caataagata
     1501 ttgatagtag cgcttttttg attgatttta aaatgtgaga tttgttcacg tataatagcg
     1561 gattttttat gtacagtctt ttaaaaagaa gcttttcttt atattgataa aaatacattt
     1621 ataaaacact attgcatcaa aatgtattaa tatgtataat tatgaatatt aattagggag
     1681 ggctgtataa atgaagtgtt taattaggtt tatattagta ttagggttgt taattagcag
     1741 tgctatggtg tatatcaatc ccacagcaca tgctgaacaa gatcaaacat gggagaagat
     1801 taaagaacgc ggtgaactta gagtgggtct ttctgcagat tatgcaccaa tggaatttga
     1861 gcatacagtt aatggtaaga ctgagtatgc aggtgtagat attgatttag ctaaaaaaat
     1921 tgcgaaagat aataatttaa aattaaaaat cgtcaatatg tcatttgata gtttgttagg
     1981 agctcttaaa actggaaaaa ttgatattat tatttccgga atgacttcaa cgcctgaacg
     2041 taagaagcaa gttgattttt cagattcata tatgatgact aaaaatatca tgcttgtaaa
     2101 gaaagataaa gttaatgaat ataaagatat caaagacttt aataataaaa aagtaggggc
     2161 acaaaaggga actgaacaag aaaaaatcgc tcaaaccgaa attgaaaatg catctattac
     2221 ttcattaagc cgtttaccag acgttatatt agcattgaaa agtggaaagg ttgaaggtgc
     2281 tgtagttgaa aaacctgtag cagaagcata tttaaaacaa aatcctaaat taggaatttc
     2341 caatgtgaaa tttaatgaag aagaaaaaga tacagtgata gcagtgccaa aagattcacc
     2401 aaaattattg tcacaaatta ataaaacgat taaggaggtt aaagataaag gattaatcga
     2461 taaatatatg actaatgctg caaatgcgat gaatgatgac agtggtttta tttctaagta
     2521 tggtagtttt ttcttgaaag gaattaagat tacaatatta atttcactta tcggtgttgc
     2581 attaggttct attttaggtg cattcgttgc gttaatgaaa ttaagtaaaa taaaaattat
     2641 ttcatggatt gcttctatct atatcgaaat attaagagga acaccaatgt tagttcaagt
     2701 atttatcgta ttctttggta taactgctgc attaggacta gacatttcag ctcttgtttg
     2761 tggaacaatt gcattagtta ttaattcctc agcttacatt gctgaaatta ttcgtgcagg
     2821 tataaatgct gttgataaag gccaaatgga agccgcacgt agtctaggtt tgaattatag
     2881 acaaacgatg aaaagtgtaa ttatgccaca agcaattaaa aatattttac cagctttagg
     2941 taatgaattt gtcactttaa ttaaagaatc atctattgtg tcaacaattg gagttggcga
     3001 aattatgttt aatgcacaag tggttcaagg tatttcattt gacccattca caccattaat
     3061 agtggcagca gcattatact ttgtcttaac atttgtactt acacgcatta tgaacatgat
     3121 tgaagggaga ttgaatgcca gtgattaaaa taaacaatct taataaagtt tttggagata
     3181 atgaagtttt aaaagatatc aatcttgaaa tcaatcaagg ggaagtagta gcaataatag
     3241 gtccatctgg tagtggtaaa agtacattgt taagatgtat gaatttatta gaagtaccta
     3301 ctaaaggtca agtgattttt gaaggcaatg acttaacgga aaaagggaca caagtagata
     3361 aactacgtca aaaaatgggt atggtatttc aaaacttcaa cctatttcca cataaaaaag
     3421 ttgtcgataa tattatttta gctcctaaat tattaaagaa agataataac gatgaattac
     3481 ataaggaagc attgtcgtta ttagataaag tgggattaaa agaaaaagca gatgtatatc
     3541 cgaatcaatt atcaggtggt caaaaacaaa gggtagcaat tgcaagagct ttagcaatgc
     3601 atccagatgt tattttattc gatgaaccaa cttcagcatt agatcctgag gtagttggtg
     3661 atgtattaaa agtaatgaaa gacctagcca aagaaggtat gaccatggtg gttgtgacac
     3721 atgaaatggg atttgccaaa gatgtaagtg acaaagtcat atttatggca gatggcgttg
     3781 tcgtagagtc aggcacacca gtcgaaatat ttgaacaacc gcaacatgaa agaacacaaa
     3841 atttcttagc aagagtatta taacaaccta acgaagcttg aatatatgat acgcaccaca
     3901 aagttatatc atatattcaa gctttttatt attaaaatta tgtgaaatga aacctcaata
     3961 tgaataatgg ttgtgttaaa ataacatagt attattttat ggggggcata cgattggata
     4021 caaagcaatt aaagcaagat atcattgact atgcatatac aattggtatc gacagtattg
     4081 gatttactac tgccgatccc tttgatgaat tgaagcaaaa gctagaagca tatcatgcaa
     4141 atggttatgc ctcaggattt gaagaatctg atattgcttt acgaacggag cctaaattat
     4201 ccttaccaac agcaaggtca atcatagcaa ttgcagttgg ttatcctaac aaactgaaag
     4261 gtgcacctaa gagtgttaga ggtgatcgca gaggcttatt tgctagagca tcgtggggtc
     4321 aagattatca tacaattatg cgtaaacgat tagacatgtt agctgcattt attgaatcta
     4381 aagttccaga tgttgaaatc aaatctatgg tagatacggg tgtattatca gatagggcag
     4441 tagcagaacg tgcaggttta ggatttgttg gtcgtaatgg ctttgtcatc aatcctaaac
     4501 taggaacatg gacatacctc ggtgaaatgt tagtcagtat accttttgaa cctgatgatc
     4561 cattattaga tagctgtggt gattgtacaa tttgtgttga tcgttgtcca acaagtgcat
     4621 tagttggaaa tggccaatta aatagtcaga aatgcattag ttttttaacg caaacaaaag
     4681 gctatatgcc tgaccaatat cgttataaaa ttgggaatag attatatggt tgcgatacgt
     4741 gtcaacaagt ttgtccgaaa aatagaggta ttaataccga acaagatgac atcattttgg
     4801 aaccagaaat tttaaagcca agattagtac ctttactacg tatgtctaat aaagaattca
     4861 aacaaacata tggtcacctt gcaggtgctt ggcgtggtaa aaagccgata caacgaaatg
     4921 ctattttagc attggcgcat tttaatgaag tggatgcaat tccagaattg aaaaaagttg
     4981 caacaacaga tgaaagaccg ttgattcgag caactgctta ttgggccatt ggtcaaattc
     5041 ttggggaaga agcaagagat tttatcaatg ctaattatga tcaagaggat gcagaagttc
     5101 agaatgaaat gataaaagga ttagacacaa ggagagaata gtaatatgac aaatcatatc
     5161 gttttatatc aaccagaaat cccagctaat actggtaaca ttgcacgtac gtgtgcaggt
     5221 acaaacacac atttacattt aattaaacct cttggattta gaactgatga taaaatgtta
     5281 aaaagagccg gtttagatta ttgggaattt gtgaatatta cgtatcatga tagtattgaa
     5341 gcattttttg aagtcacaaa tggcgagtat tacttattaa caaaatttgg taaaaagact
     5401 tatagtgatt ttgatttttc aaatcatgac aaagattttt acttcatttt tggaaaagaa
     5461 acgacaggat taccagactg ggtgaaagag aaatatcaag acacagcgtt aagaattcct
     5521 atgagtgaac atattcgttc attaaattta tcaaatactg ctgcgttgtt aatttatgaa
     5581 gcgttacgtc aacaagattt tccgggattg aattaaaatt tactcatttt cattataaaa
     5641 atgatgctta agtgctatat ttagagtata agcatgttaa agttaaatgg tttgatatat
     5701 tgaaagttag ggtataataa ttattaatat cgccgttttt atgtaaaatg caaactaaga
     5761 ggagtgtaga aatcatggca atgaacttta aagtctttga caatagtcaa cttgtagcag
     5821 aatatgctgc tgatattatt agaaagcaat ttaacaataa tcctactaca attgcaggtt
     5881 ttcatttaga tacagatcaa gcgccagttc tagatgaatt aaagaaaaat gttgaaaaac
     5941 atgctgttga ttttagccaa ataaatattt tagattatga cgataaaaaa tcatatttcg
     6001 aagcgttagg tgtaccagca ggtcaagttt atccaattgc ttatgaaaaa gatgcaatcg
     6061 aattaatcgc tgataagatt aaaactaaag aaaataaagg gaaattaaca ttacaagttg
     6121 tttctatcga tgagcaaggt aagttaaatg ttagtattcg tcaaggacta atggaagcaa
     6181 gagaaatttt cttagtagtg acaggtgcta ataaacgaga tgtagttgaa aaattatatc
     6241 aagaaaatgg taaaacaagc ttcgaaccag ccgatttaaa agcacataga atggtaaatg
     6301 ttattcttga taaagaagcg gctgcaggtt tacctgaaga tgttaaagct tactttacgt
     6361 cacgctttgc ttaattaaaa atctgtgagg tattgattat gtctaaagac aaagatccaa
     6421 aattaaatta tcatgaagaa gaaaacagta tggtaacgga ttttgaagat ttaaaagaat
     6481 taggtaaaga aatggaacaa atctctgatc aaaatgatca agaaaagaat tctgaagaag
     6541 acagtcagta aagtattata attgtgtaat attcagttat ttatcatgta aataatgtat
     6601 agtatagaac atatatcaaa taggattgat tatcttgaat atgaagttag gtagcatttt
     6661 gttacctagc ttttttatta tattcataga agctgacttt ctgctagttt cagtgttggg
     6721 gctgtttccc caacttacat tgtttgtaga aattgaggat ccaatttttc tatgttgtgg
     6781 ccccgccaac ttgcatgtta ttttaagctg actttccacc aacttctgag tttgtggccc
     6841 gtttccctaa tttgcaatgt ctgtagaaat tagaaatccg atctcactat tttggggcct
     6901 cgccaacttg catgttattt taagctgact ttccaccagt ttctgagttt gtggcccgtt
     6961 tccccaattt gcaatgtctg tagaaattgg gaatccaatt ccactatgtt ggagctccac
     7021 caacttgcat gaaatagaag ctgacattcc gaagcctcta tgttgtggac tgcagtcaac
     7081 tactgccaat ataactttgt aaagcataaa acattgattt gtgtaccaga catagtaata
     7141 tatcaaaagt tcaattttaa atattaaaac accatttaaa cggaattgat aattaattac
     7201 tccagacata attgaaagaa gtcatctatc agtaatattt ttaatgaaat cattaaaccg
     7261 cgtttcttaa tatgccaaga ttatgatatg ctattgaaga atgaaattag aggagattta
     7321 tatatttatg ttaggaaaat actggattca attattgata gcaacagtta t
//
LOCUS       DANJFZ010000122         7218 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.122, whole genome shotgun sequence.
ACCESSION   DANJFZ010000122 DANJFZ010000000
VERSION     DANJFZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.122"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(254..946)
                     /locus_tag="PWN45_002325"
     CDS             complement(254..946)
                     /locus_tag="PWN45_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL0624070.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1182..1640
                     /locus_tag="PWN45_002326"
                     /pseudo
     CDS             1182..1640
                     /locus_tag="PWN45_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1627..1929
                     /locus_tag="PWN45_002327"
     CDS             1627..1929
                     /locus_tag="PWN45_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624071.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2083..2442
                     /locus_tag="PWN45_002328"
     CDS             2083..2442
                     /locus_tag="PWN45_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624072.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2527..3138)
                     /locus_tag="PWN45_002329"
     CDS             complement(2527..3138)
                     /locus_tag="PWN45_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL0624073.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3217..3693)
                     /locus_tag="PWN45_002330"
     CDS             complement(3217..3693)
                     /locus_tag="PWN45_002330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDL0624074.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3696..4196)
                     /locus_tag="PWN45_002331"
     CDS             complement(3696..4196)
                     /locus_tag="PWN45_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624075.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4431..5198)
                     /locus_tag="PWN45_002332"
     CDS             complement(4431..5198)
                     /locus_tag="PWN45_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL0624076.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5195..5887)
                     /locus_tag="PWN45_002333"
     CDS             complement(5195..5887)
                     /locus_tag="PWN45_002333"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDL0624077.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5904..6566)
                     /locus_tag="PWN45_002334"
     CDS             complement(5904..6566)
                     /locus_tag="PWN45_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDL0624078.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2402 a         1295 c          994 g         2527 t
ORIGIN      
        1 atttataaat agttaattta caactaaacg ataaatatta tatgcaaaat acatctttaa
       61 tattaaagta ataccaatat tttttcaata aacctagtgt aatatatgtg taattctaaa
      121 agattcttct ttaaaaatat aaataccacg acatattgct ttaacatttt catttataaa
      181 gcgaaaaaat gcatcgctac taagttgaat gtttagtaag gatgcattta attcactaaa
      241 atgattaaat tacttatatc ttttcatctg attgattatc gaaatttctt ccttctaaac
      301 ctgctaactc ttctttagaa gctgcaggtg ctttcatttc aaatatctca ttcactactg
      361 tgtaatcgta atatcctaat ctggcaatag gtttaatcga cttaatgtcc aatttaccat
      421 tatcaagaat aaccttatcg tcaatatgaa cttgggcaac tcttcctata acaatatcta
      481 cggtagatac tggatctcca gttggaatac gaatcgtttg aacgtactca cattcaaaat
      541 gaactggcga ttcttttaca cgatatcctg gagcttctat acatttttcc tttgttacat
      601 ctgcaaaatt aaattcatcc tcttctggtg gcaatgcttt cgatgataaa ttaactgctt
      661 ctcttaaatc atacgttgcc atattccaca caaaccaacc tgtctcttca gcatttttca
      721 ctgtatcttt acgttcgtga tcaccaagaa cggattgatt tgctgcgaac ataaccatag
      781 gcggatccca agttaagttt tgatactgac tataaggcgc taaattatct ttcccatctt
      841 tcgatacagt agagatccac cctattggac gtggtactgt actactttta aatgggtcgt
      901 gcggtaaacc atgacttctt acaccttgtt ttggcgaata attcatacta tcttcacccc
      961 ttataagtaa ttacatttaa ggttacgccc tctttacata agcgtctaat ataaataaac
     1021 aatttattta taagtagaaa ctatatatga cgtggttgct tataatttgc gttcttgatt
     1081 cgaaaaattc agataaggat ttatacaatt aatatttatg atatcttttg taaatttaat
     1141 taattatagt tacttcaatc atgattagtt tataataata aagtgaaatt gaaaaagaca
     1201 gctattatgc gatgagcgaa aaacttcaag taaaacaaga atttaacggc gttacaaggc
     1261 aatacttgaa tttcagaaca gatagtatcg atgatctgga taagttcaaa aaagaattca
     1321 aacctgttat gcacttaaaa ggtgatgcat tcaatcaaca gttacaatct ttgattaaca
     1381 aatatccaca aatacaaaaa aatatgaaat cagagttcat tgcttattat gataaagaaa
     1441 aaaatagaga aacagtaaaa aactatgctt ggaaccttca aaaatctata aatgacatta
     1501 tgcaatcata tcctagcaca aaatttgtac agttttataa agatgatgtt tccccgtcaa
     1561 tggtagatgg aaatggccgt ttaaaatcgg atactaatgt aatttccatc gagggaggaa
     1621 agtatgatga gaacaaatga tcatataacc gatttagatg ctattgagtt aagtagcggt
     1681 tggaattaca attcgaaatt ggagactggc aaaaatgcac tactatcaga tagcctttta
     1741 agagatatta aagtattaag ttctaatgct aaaggcgacg ctgacatact agaaacagaa
     1801 cttcgtgctg taataaactc aatgtctcaa aacgaaggta aatctgttga tgcacttaaa
     1861 aatagtaccg cttctctaaa aaacaatcta accaaattaa aagagcaact cgacaaaact
     1921 acacattaat ttcatacatt ttgagtgaac agaattatat ttccatagca aacattccta
     1981 aactacacat acgttacata attgattcat ttttatagaa acgggtaaaa atgataaagt
     2041 aatcatcttt cacaaaaaat caaatattgg gaggaattaa atatgatttt gcgttacgca
     2101 acgaatttaa aacttgcgaa agagctatac caagccagta aacctaaatt gcaagaagat
     2161 aagggtatga ttgaactgtt tgaacatact tttggcttgc aaaaagaact tgtaaaatac
     2221 gtcggcattg cagaagcaac tactgctgct ttatactcag ctagctttat caataaaaac
     2281 atttctcgcc ttgcatcact atcaacgata ggcattttaa gtgtagctgc ttacaaacat
     2341 ttcgaagctg gccaaggtaa aaaaggtgca caacatgcat taaatttatt aggtcttgct
     2401 actttaagtt tactagatac attttcttgt aataaaaaat agtcttcatt ttagaagcac
     2461 atactttagc acctgtgtac ttttgattca attcataaac ggtataccgc tatagcatgt
     2521 gttttattat ttttctggtc tctttatcgt aaatattccc cctaatttag cataatcatt
     2581 tccggctaaa cgagaaacag cctgtaatgc gtgtggattg attttaaaat caccctcata
     2641 aatactgtca tcaatatgat aagttacaat ttcacctatt attaaatcag ccccatctaa
     2701 tacatctcca agcaatatca tttgcgatag tttacattcg aatctcattt tcgcatcttt
     2761 aattcctggc gtcttaatcg ttgtagatgt taaaagtgat aattctgtac gactcaactc
     2821 actgtcacca tatgctaacg gcgctgcagt ctcattaata tcttgaacat tatcttcgtc
     2881 tgtaatatgc acaacaaagt ctccagtccg ttctatattt aatgcagtat cttttctctt
     2941 acctcctgca cgttgaactg caatagcaat cattggcgga tgattattaa caatattaaa
     3001 aaagctaaat ggtgctgcat ttactgatgc atcttgattt aatgttgtaa caaaagctat
     3061 aggtcgtgga ataattgaac caattaataa tttatagttt tctctagcag ttaatgattg
     3121 tgcatcaaac gtatacataa tacctacctc ttttctaagt atatctaggt atttctccga
     3181 ttttggttaa tttaaacatc tattctcctc tgaaaatcac ttgtatttat ttagcaaatc
     3241 ttttgaaata tgacacatat gcatatcttc tggatatttt tctaaatgtt gctgatgttc
     3301 ttcagcactt ttaatgtagt tagacagcgg taagacttcc actgcaattt gatctctgtc
     3361 tttacgtcgt tcaatgaact gacgcgcttc aattaagtgg tcatctacac aactatataa
     3421 acccgttcga tacttttgtc caatatcatt tccttgttga ttcacactgt aaggatcaat
     3481 gatttcaaat aaataattca taatgtctgt aattgttaac atacgatcat cgaaatgaag
     3541 tttgacacat tcagcataac catcatacgg accgtctaat ttagagcttc ttccatttgc
     3601 tcttcctgct tctgtatgta taattccagg tattgttgca aaaaatgctt caacacccca
     3661 taaacatcct cctgctacat aaacaactgc catatttaca cctcatcatc cttttttata
     3721 tttttaacaa ggttatacca tttaataccg ccatgacatg attctgatac accttcatta
     3781 cgatacccat atttttcata aaatgaaatt aatgattctc gacatgttaa cgttacacca
     3841 tgtcgatgat gattcttagc aagagtttca aaatagttta gtaagcgacc tgcaataccc
     3901 tgaccttgat aatttggtgc tacaacaaga cctaacacac taatatagcc accttcacta
     3961 ttatttgtgg agacattttt aaataaatca tcgctaatgt aacgctcttt tatgactgga
     4021 ccgttgatgt atccaattac ttcatcattc ttttcagcta caataaaagt ttcttgaatt
     4081 tgttcaattc tcaatttcaa tgcctctcgt gttgcagctt cttccggtga aaaaccaata
     4141 ttttcaagtg caataatttg atctaagtcc tgcatgatta cttttcttaa taccataaaa
     4201 acacttcctt agccctatat atcaaaagta ataccttcat ctttatattc attattttca
     4261 aactaccttc actataaaaa acgagtcttt ttattgaaag ccattcgcct taatcaatta
     4321 gacaagttgt attatacgaa ttagtaaata atcatgatta taattcattt taagcatact
     4381 caaaaagact ggtacatgta taccagtcta attcgaaaaa atatatttag ttaaaaccat
     4441 ttcaaaaacg acttcacttt tcctggctta gtcggtctgt ctaaataaac aaaattatca
     4501 ttgattactg aactggcatt tcgaagtaat gcttcaatat cattcgaata tttcttcaat
     4561 ttatgattgt gaaataattc ttgcatcaaa aatggtcttt ggtcacatga atgtgcatct
     4621 gaagctacaa aatgagccaa attacattct ataaattgta atgataactt ttgaatgttt
     4681 ttaccaaatc caccaactaa agaactcgat gttaattgac tcagtgcccc atttgcaacc
     4741 aattcatata atatttccgg atttttggcg atacttctat ttctttcagg atgtgcaatg
     4801 attggtatgt aacctctcga ttgtatttca aaaaacaatt gttttgtata atgtggtact
     4861 tcgcccgttg gaaattcaat taataaatat ttcgaacgat taataccttg aatactacca
     4921 ttatctaagc ctttcagaat cgaatctgta attctaattt cttgcccggg aaataattta
     4981 atatccaatg cttgaacttc tggatgcgtt cttaactccg ccaatttcac aagcacttgt
     5041 tgaaatgtat tatcatatct cggatgcaaa tgatgaggtg tcgctacaat acttgttaca
     5101 ccttcatcct tagcttgctt taatagtgca atactctttt caattgtttt aggaccatca
     5161 tctatatcaa ctaatatatg gttatgaata tcaatcatga ttcatcagtc ccataatatg
     5221 catagtaact agcactttta tctttaggca ttctatttaa gactacacct aataatttag
     5281 cacctgttgc ttcaataagt tcttttcctt ttttaacttc atctctatta ttattttccg
     5341 aattaactac gtagacaaca ttgccggtaa actttgaaaa taattgcgca tctgtaactg
     5401 tgttcactgg tggcgtatcg ataattacaa agttataatt catcaataat gtgtcataca
     5461 aatttgcaaa tgcccttgat gtaattaact ctgacggatt cggtgggatt ggcccagacg
     5521 tcaagacgtc taaatcttga atttcagttg agataatact gtcttgataa gttgaccaat
     5581 ttagcaataa acttgatagg ccttcattgt ttggcaaatt aaaaatataa tgctgcgtag
     5641 gtttacgcat atccccgtct acgattagtg ttttataacc tgcttgcgca tatgcaactg
     5701 ctaaatttgc tgcaattgta gacttacctg cgcctggtgc ctctgaagtg attacaatgc
     5761 tttgaactgc actgtcagga tttgcaaaca taatatttga tcttatgcct cgaaatttct
     5821 cgctaatagg tgactttggt tgttcatgga caattaaact tgatgtactt cttcgtgtat
     5881 tcgtcatggt aattcctcgt aaattaaaat ttttgtattg aacctaaaat aggtaatcct
     5941 agttgcgatt caacatcttc ttctgtctta atacgcttat ctaataattc ttttaagaaa
     6001 ataatcaata ttgctaaaac aataccaaca ataatgctga taactaagtt gacagatact
     6061 attggagata cttttacagc attatcatgt gctgaggaaa gtatcgtaac attatcaaca
     6121 ctcataattt taggcatgtc atgagcaaaa actttagata ttttattaac aattttgtca
     6181 gattcagatt tattcccagt ggtaactgat acagtaataa tttgagagtt tgtttgattg
     6241 gttactttta aaaatgaatt caactcagct gttgaatact gaccatcaaa ttctctagat
     6301 actttatcta gaattctagg acttttgata atttccgtat atgtattaac agactgcaaa
     6361 ctactttgaa cattttggaa agctaaatca cttgaggact ttttcatgtt cactaatatt
     6421 tgagtagaag cagtatattt gtcaggcata acaaaaaagg ttaatgccgc acttactaca
     6481 agacatattg ccggtaaaat aagcaataat ttaatattct tctttagaat atttaatagt
     6541 tttactaaat caaacttttc tttcatggtt tcctccacat aatcaatcat tgtattcatt
     6601 atgtatgttt tataaatcgg acaattatat ctagtttaac gaccacaaaa catacacaac
     6661 tacattttct ctaattattt atataaatat tttatcgttt aaaattatat catgattctc
     6721 taccattatg tataacttat ttatattttt gcacaagata taatattgtc caactttaaa
     6781 tatccaaacc tattaataat aaaactagat accatcgtac tctatcatgg ctttcttata
     6841 atcgagtaga agcatcatca ttacttgatt atttgctctt tacaacaccg agcgtgcccg
     6901 tactcggtaa ttcaatacct tgcgtaaccc gtcactgtga gttgggttaa tgataataaa
     6961 gcccacacct tttaaaaaga tgtgggtaat ttatataatt tttatttaca tttttaactt
     7021 ataaaaaaag cgcctatgtc atgatttacc atcacatagg cgcttatcaa taaattatta
     7081 cttattactt tccatttcat ctaatttatg cggatttcct gtaattagat gacaacttat
     7141 tcttttcagg ggaacattac acttttataa tatgttcaaa gacaaactta accattcaca
     7201 aatataaaga ataatatt
//
LOCUS       DANJFZ010000123         7153 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.123, whole genome shotgun sequence.
ACCESSION   DANJFZ010000123 DANJFZ010000000
VERSION     DANJFZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.123"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            44..850
                     /locus_tag="PWN45_002335"
     CDS             44..850
                     /locus_tag="PWN45_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0624079.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(913..1569)
                     /locus_tag="PWN45_002336"
     CDS             complement(913..1569)
                     /locus_tag="PWN45_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0624080.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1682..2155
                     /locus_tag="PWN45_002337"
     CDS             1682..2155
                     /locus_tag="PWN45_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0624081.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2278..3489)
                     /locus_tag="PWN45_002338"
     CDS             complement(2278..3489)
                     /locus_tag="PWN45_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDL0624082.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3671..4330)
                     /locus_tag="PWN45_002339"
     CDS             complement(3671..4330)
                     /locus_tag="PWN45_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624083.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(4390..5532)
                     /locus_tag="PWN45_002340"
     CDS             complement(4390..5532)
                     /locus_tag="PWN45_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0624084.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5800..6186
                     /gene="gtxA"
                     /locus_tag="PWN45_002341"
     CDS             5800..6186
                     /gene="gtxA"
                     /locus_tag="PWN45_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDL0624085.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            6320..6427
                     /locus_tag="PWN45_002342"
     CDS             6320..6427
                     /locus_tag="PWN45_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0624086.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         2370 a         1110 c         1319 g         2354 t
ORIGIN      
        1 gcataataaa taacatttta atttgagtaa agaaggtaag ggtatgaaga tagggacaat
       61 cgccgattta catattgatc gtcataataa aaaaacatcc gaagattact tagaggcatt
      121 ggttgaaata gttaagtata agaaattgga tatattgttg attgcggggg atatctcaaa
      181 tcattatcaa ttgacgcacc aatttataac gcaattgaca aaacaattag atatacctgt
      241 gaaatttgta cctggaaacc atgatttatg ggaagttgaa agtatgacta cgcaagacat
      301 ttggaataat tataagagta tgtcacagtg cttggtagga aaaccattta tagtaaatga
      361 agaatgggca atcataggac atactggctg gtatgattat agctttgcag cacaacgatt
      421 ttcattagat gagttacaaa aaggaaaaca ttatggtgcg acttggcaag ataaagaacg
      481 aatatcttgg ggcatatcag atcaaaattt atctaaaata gcggctgaac aagtgaagaa
      541 agatatatta gaagtaggaa atagacgagt gattttagtc acacatgttg tgacgcaccc
      601 tgatttcatt gttcctatgc cgcatcgtat attcgatttt tataatgcat ttattgggac
      661 aagtgatttc aatcctttgt atgcgatgtt cgatatacca tatagtatta tgggccatgt
      721 tcattttcgt aaaagtgtga tagatgatgg cagatgttat ctctgtccgt gtctaggcta
      781 tccaagacaa tggcgttcag aagatattta ccaggaaata aatgagacga tacaaataat
      841 agaaatttaa aatgcgcaaa cctgacccag tttgcgcatt ttatgtttta cacacgcgag
      901 taatgtgttt acttacgtgt gtttattttg ttgctgattt tcaattgtat atgaatgtgg
      961 ttgcacataa atgcactttc ttcctggtga attaaagctg tattccattt tctctttacg
     1021 aattttaata atttgtttgc gatttggaat gatggcaggt aaaactaggc cacgacgaat
     1081 atgactccaa agtggatcga tataatagta atagtaatca tcgtacccaa tgagtaacgt
     1141 tacgtgaata tttgaaactt gtttcgctgg cttgttatca aagcggaaaa cacgtagtaa
     1201 tggtttagaa ccaagattag tatggtatat taacacaggt tgaccttgat cgataatacc
     1261 tttaagatct tctaacgatt taccagtgcc gtctacgata ttaggattgt atttttgtaa
     1321 aaatggtaca tatgcttctg gaaatatcgt ttgatgataa ttgccaagct taatgaataa
     1381 gtgatgtcca acataacctt tatgtggatt gttcggatgt gtcggccaat gtctcataat
     1441 ttctgtagca gggatatgtt ggttgttgta ttgcaacatc atggctgcgg aaacaccttc
     1501 acaccccatg accataggga taggaaatag ctgactgata ggtttaactg gtaatatttt
     1561 tcggttcata atatagtcct cgcattgatt caataaatat ttaatataat tatatagcgt
     1621 caatgcaaaa tgtcctaaac atatgtttta catgagtgaa taaaattaat ggagtgataa
     1681 aatggaatat caattacaac aacttgcgtc gttaacgtta gtaggtatta aagaaacgta
     1741 tgaaaatgga cgacaggctc agcaacatat agcagggttt tggcaaagat gttatcaaga
     1801 gggagtaatt gcggatttac agttaaaaaa taatggtgat ttagccggga tacttggctt
     1861 atgtatacct gaattagacg gtaagatgtc atatatgatt gcagttaccg gagataatag
     1921 tgctgatatt gaaaaatatg atgtcataac attagcaagt tcaaagtata tggtatttga
     1981 agcacagggc gcagtaccta aagcagttca acaaaaaatg gaagaggttc atcactacat
     2041 acatcaatat caagcagata cggtaaaatc agcaccattt tttgagttgt atcaggatgg
     2101 tgatactaca agtgaaaaat atattacaga aatttggatg cctgttaaag ggtgattgaa
     2161 tagaaatgtc cttgctatga aggcgtaatt ttagttgaag attcgatctc ataattcata
     2221 aagaagaacc ctgacaaaga tagatatctt tgctagggtt ctaattatgt tgaagtgtta
     2281 tgcgacatgt tgctttttaa tcattttaaa gtaaatgatt tgtagactga ctaatagaat
     2341 ggctgtaatg aagataataa tcatatatgg tgatgtatta aattcgcctt ttaagccaac
     2401 taatggtgca acagcgccac ctaagataaa ttggaataag ccaagtaaac ttgatgcgtt
     2461 accactgcca cctgttcgtt cttccatagc cattgtgaaa ccaagcggtc caattgacgt
     2521 cacaggacac acatttaaga agaatgcgat gagtaagacc caaagtggta aatggaatgt
     2581 aagtgtcagg ataattaaag caacacctac cacttgtata atagttaaaa tgattaataa
     2641 tatgtggcga tgtaattttt ctactaataa agcaacgact tgactgacaa tgattaaacc
     2701 tacaccgtta acagcaaaca tgatactaaa ttgttggggt gtcatattat acaatttttg
     2761 agtaataaat ggcgatgcag atgaataact aaatagcatt acataagtta aaccttgtaa
     2821 tagcattgga ataataaatg ctggtttttt caaaagactt ccaaaatctt taatgacgct
     2881 actaaaattc acctgcttta attcatcttt agatgtttta ggtaattgag aagcgacgcc
     2941 aattaaaatg atgagtgcca caatagttaa aattgtgaaa atagaacgcc atgttgctac
     3001 ggataaagct aatccaccgg ctaatggtgc aagaatagtg atgatgccat taactaccat
     3061 taaagatgct aaaaatttag cgagtgcgtt gccactaaat ttatcaccag cagaggcttt
     3121 tgcaatcacg atgacgccac caccagttaa accttgaata aatcgtaaag ttaagaataa
     3181 tggcaattga tcaacaaaca tagacaaacc tgataccaaa atgaaaatga tcaaaatgga
     3241 tacagcaatc cgtttgcgac caaaagcatc ggataaaggt ccaaagataa attggcctag
     3301 tgcaagacca atcattgtga atgataatgt taattgaatt tctgatgtag tagaaccaaa
     3361 atcaccttgc actttaggta atgatggtac gtacatatct atagttagtg ggccaaaagc
     3421 ggtcatgacg cctaacgtaa ttagcaataa aaatgaaaaa tgcttcgttt tggttttcgt
     3481 tgattgcatg atcagactcc tttaaatgat ataattatat aaattatata cgttttgtgt
     3541 gtatttgtat gtacttagaa tacaagttta tataaaaaat ccctatactc ttaatgcatc
     3601 atcacgaata acgtagtgat acagataaag tatagggaat taatgtttat catcaaagca
     3661 ggtgtaataa ttaattagcg ccaccattgt catctttaaa taagctttct aatttagatt
     3721 taatactgtc gaattttgta ttaatatcat ttaacatgct ttcaatttta ccttggcttg
     3781 cattgttttt aagtgcatta tttgcctctg tcaatttgtt gatttggtct tgataaaatt
     3841 gagttagctt agaatcagct ttgttttgtt cttgctgctt caagttgtcg atttgatttt
     3901 gtaaattttt aataatgtca tcagtttttt ggttgttttt aatattttct ttagcattat
     3961 ctacttgctg gttaatttgg ccgtcttgat tttcaacttg ttttttataa ttttgagaga
     4021 ctttagggtc attactaact ttatcgctat cattgttaag taaggcgcgt gtgacaaaaa
     4081 tagcgacaat aattaagaga atggcaataa tgatatataa ccattttttt gaacggcggg
     4141 atttctcgtt ttcttcttca agaatttgtt ctcttcgata atctctacca ttatagtatc
     4201 gttccgggtg ttcttcagga tcgcctttag catattgttg tctatacgat gcgtcttcag
     4261 attgacgacg atgattttgg ttttgattgt cgtattgata tgaatcacgg tatttatcag
     4321 ttcttttcat gggtttcacc tcattcaagt tttaactgca aataataaag ctgatgattt
     4381 actttgacat tattgtaatt tcagaatacg agcaatgtca gttgcagtgt ttaataaatt
     4441 ttgttcgcta ttttgtagga catcttctaa agtgctaggt gaagagatta tagaataggc
     4501 actatcaata ccgaaatcgt aaacatgttg ataattttcg cctagactgc cacaaatcgc
     4561 gatgacagga atatgatatt gttttgcagc taacgcaacg cctacgggtg ttttaccaaa
     4621 gatggtctga taatccatgc gtccttctcc agtaataacg aggtctgcat ctttaattct
     4681 ttgatgaaaa tctgtaatgt caaagacgac atcaatacct tttgttaaag ttgtctcaca
     4741 aaacgctaat aatgctgcgc ccataccgcc agctgcacca gaacctggta tttgattaac
     4801 ggactttcct gtgcacattt ttatcttatc atgataatgc gacattgcga aatccaactt
     4861 tggtatcatc tttgcatcag cgcctttttg aggaccataa atataggtag caccattttc
     4921 acccaataaa ggatttgaaa catcacaggc cactttaaag gtcacctctt ttaatcgcga
     4981 atctagattg gttatatcga tttgtgcaat gtgagcaaga ttagcaccat tcatttgtaa
     5041 taagtccccg tttacatcag taaactttac gcctagtgca cttagcatac ctgtaccacc
     5101 atcatttgtt gcactgccac caatccctaa aataatggtc ttagcaccat gatttaatgc
     5161 atctttaatt agttcaccgg taccatatga tgatgtgtat aaaggattac gttcctcttt
     5221 ttctaataaa tccaaacctg acgctgccgc catttcaata attgcaattt gttgttcgtc
     5281 tgcgcgtgca taacatgctt caataggtcg cattaaaggg tcattaacga tgactgtata
     5341 cttagtggca cctgttgcat gcattaaagc atctgtggta ccttcaccac catcagccat
     5401 cggaatgata tcataatgaa gggtattccc ataaacatta gtaaaagcct gttttataat
     5461 attgccaact tgctgtgcgg tcatactttc cttaaatgag tcaggagcga tgacaatttt
     5521 ttgtaattgc atagtagaca tcctctcatt actgttgtcg ctcttattgt atataaaatt
     5581 actagtgaac taaagtaatc tctatagtgc taatgagtca cgcgataaat cagttacatt
     5641 ataatggcgt ataaggatga ggggaaagag actaaatttg ctgtgaaagg gtataaagat
     5701 tgaaataagc atatttgaag tgaagcggat tggctcgcat attcgagtat gttcgagaac
     5761 gtagttatgc atatattttg atgttgagga gttacacata tgaaactaac acaaacgcat
     5821 gctgaaattt taaaatttat cattgttggc ggcattaata cgttaaatta ttatgttgtc
     5881 tatttattgc tgttaaaatt attacacatt gaatatatga ttagtcatat taccggattt
     5941 ctagtcgctt ttgtgatttc atattatttg aattgttatt ttgtttacag ggtaaaacct
     6001 acttggagaa aattcattag ttttccaatt acgcagattg tcaacgtaag cttacaaaca
     6061 gttttattat atgtatttgt atcctggcta aatttgccag cagaaattgc accatttgct
     6121 ggtttaatta ttacgatacc catcacattt atattatcta aatggatttt aaaagatagc
     6181 aattaaagta gcgtaaacca ctaaaaaaaa ctttacttaa gccttaatat tgcgtgaaat
     6241 ataataaata aatgatataa tatgatggtc gcctaacatc tcgggcgtta ctttaacgca
     6301 gagaggaggt gtatgacgga tgttcaattt attaattaac atcatgactt cagctataag
     6361 cggctgtctt gttgcgtttt ttgcacattg gttacgaacg cgcaacaata aaaaaggtga
     6421 caaataacca acacctaaat aaatcccctc actatggcag tagtgagggg atttggtgta
     6481 tttagatgtt caatttatta tgtaattaat tatagcattg atgaacaagg tgtgcaatgt
     6541 atttttgcaa tattacaata aatgaattta attatattag aattaagtat ttcgaaaaat
     6601 gatttaatag ttgagtcata ggatcattag ataaagcgaa taatcaatta tagggacatt
     6661 gggatacaaa tcaatgtact agactctaca aggctatatt cagggaccca acaaagagaa
     6721 attggattcc caatttctag agacaatgca agttggggtg ggacgacgaa ataaattttg
     6781 tgaaaatatc atttctgtcc cactcccaga accactacat aataaatcat tagtggctct
     6841 ttatcatttc tgccccataa ccttgtgaat ttttaaaaga aatgtgtcac aaatccacgc
     6901 gacattgata atcgttatca actaatatat gatgtacttg taataagtat taatttttaa
     6961 caaataacaa atttatatta tgtatatcat ccagtgaaat aaataatata agcggcttaa
     7021 tttagattat taattgaaag gagttacata tacgatgaac aatcagacaa attggataaa
     7081 gatactcagc ggatttgctt ctgatagtaa atggaaaatt atgttatcaa ttttgttgtc
     7141 tatcatcagt gtc
//
LOCUS       DANJFZ010000124         7111 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.124, whole genome shotgun sequence.
ACCESSION   DANJFZ010000124 DANJFZ010000000
VERSION     DANJFZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.124"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..945
                     /locus_tag="PWN45_002343"
     CDS             <1..945
                     /locus_tag="PWN45_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDL0624087.1"
                     /translation="ISILIESIALIIVFLYFFNLNIIDIIFLILLNILMCLRIYLNVF
                     FRMTLKYNQILYIALIQFLGLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTI
                     GEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLA
                     TFLYPINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNI
                     DSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTI
                     AFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD"
     gene            946..2151
                     /gene="cap8L"
                     /locus_tag="PWN45_002344"
     CDS             946..2151
                     /gene="cap8L"
                     /locus_tag="PWN45_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDL0624088.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            2162..2719
                     /gene="cap8M"
                     /locus_tag="PWN45_002345"
     CDS             2162..2719
                     /gene="cap8M"
                     /locus_tag="PWN45_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDL0624089.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            2719..3606
                     /gene="capN"
                     /locus_tag="PWN45_002346"
     CDS             2719..3606
                     /gene="capN"
                     /locus_tag="PWN45_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDL0624090.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            3660..4922
                     /gene="cap8O"
                     /locus_tag="PWN45_002347"
     CDS             3660..4922
                     /gene="cap8O"
                     /locus_tag="PWN45_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDL0624091.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            4999..6144
                     /gene="cap8P"
                     /locus_tag="PWN45_002348"
     CDS             4999..6144
                     /gene="cap8P"
                     /locus_tag="PWN45_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDL0624092.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(6209..6535)
                     /gene="isdI"
                     /locus_tag="PWN45_002349"
     CDS             complement(6209..6535)
                     /gene="isdI"
                     /locus_tag="PWN45_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDL0624093.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(6542..6925)
                     /locus_tag="PWN45_002350"
     CDS             complement(6542..6925)
                     /locus_tag="PWN45_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL0624094.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
BASE COUNT         2474 a         1046 c         1299 g         2292 t
ORIGIN      
        1 atctcaattc tgattgagag tatagcttta attattgtat ttctttactt ttttaatttg
       61 aacatcatcg atattatctt tttaattcta cttaatattt taatgtgttt aaggatttat
      121 ctgaatgtat tttttaggat gactttaaaa tataatcaga ttttgtatat tgctcttatt
      181 caatttttag gtttgctgat aggactattt ctatattatt taacccaaaa ctggattgtt
      241 tgttttatta ccagtgaatt gtttgcaacg atatatacat tggttaaatt acggggatta
      301 actataggcg agtatcaaag tgaagataat aatgtggtaa aagattatgt gatgctactg
      361 agtacaaata gccttaataa tttgaatctc tatttagata gattaatctt attaccaatt
      421 ataggtggaa cagctgtaac tatatcattt ctttcaacat ttattgggaa aatgttagct
      481 acatttctat atccgattaa taatgtagta ctttcatata tttctgtaaa tgaaagtgac
      541 aatataaaga agcaatattt gaaaactaat ctaattgcta tagctgccct atgtttagtc
      601 atgattatat gttatccaat tacaataatt attgtctctt tactgtataa cattgattca
      661 agtttatatt cgaagtttat tattttaggt aatataggtg ttttattcaa tgcagtgagt
      721 attatgatcc aaactttaaa tacaaaacac gcatcaataa cattacaagc gaattatatg
      781 acgcttcaca cgattacatt tatattcata actattttaa tgacaattgc gtttggtcta
      841 aatggattct tttggacaac gctgttcagc aacattatta agtatgtgat tttaaatatt
      901 ataggtttaa agtctaaatt cattaataaa aaggacgtcg attagatgag tgaaaaaaag
      961 attttgattt tatgtcagta tttttatccg gaatatgtat cttctgcgac gttaccaact
     1021 caattggcgg aagatttaat tgcgaatcac attaatgtcg atgtcatgtg tggatggcca
     1081 tatgaatata gtaatcataa acaggtttct aaaaccgaga tgcatcgtgg tattcgcatt
     1141 cgacgtctca agtattcgag gtttaataac aaaagtaagg ttggaaggat catcaatttc
     1201 tttagtttat tttcaaaatt cgtgattaat atacctaaaa tgttgaaata tgatcagatt
     1261 cttgtttact ctaatccacc aatcttgcca ttaataccag acgttttaca cagactgctt
     1321 aagaaaaaat attcttttgt ggtgtatgat atagcacctg ataatgcgat taagacaggt
     1381 gcaactcgtc caggtagcat gattgataag ctgatgcgtt acattaatag acatgtctac
     1441 aagaatgctg aaaatgtcat tgtccttggt acggaaatga aaaactactt actaaatcat
     1501 caaatttcta aaaatgctga caatatccat gtgattccta actggtatga catgcgtcaa
     1561 ttacaagaca atcgtatcta taatgacaca tttaaagctt accgtgagca atacgacaaa
     1621 attttattgt atagcggtaa tatggggcag ttacaggata tggagacact tatctcattt
     1681 ttaaaattaa ataaggatca gtctcaaacg ttaacaatac tttgtggtca tggtaagaaa
     1741 tttgcagatg tcaaaacggc aatagaagac catcgtattg aaaatgttaa aatgtttgag
     1801 tttttaacag gtacagacta tgctgacgta ttaaaaattg cggatgtatg tattgcatcg
     1861 ctgattaaag aaggcgtcgg tttaggcgtg ccgagcaaga attatggcta tcttgcagct
     1921 aagaaagcgt tggtactcat catggataag caatctgata tcgttcaaca tgttgaacaa
     1981 tatgatgcgg gtatccaaat tgataatggc gatgcacatg ccatttataa cttcatcaac
     2041 actcactcga gtaaggaatt gcacgagatg ggtgagcgcg cacatcaact gtttaaagat
     2101 aaatatacga gagaaattaa tactatgaag tattacaatc tgttgaagtg aggagataat
     2161 tatgaagcga ttattcgatg tagtgagttc aatatatggt ttagtagttt taagtccgat
     2221 tctgttaatt acagcattac taattaaaat ggaatcacct ggaccagcca ttttcaaaca
     2281 aaaaagaccg acgattaata atgaattgtt taatatttat aagtttagat caatgaaaat
     2341 agacacacct aatgttgcaa ctgatttaat ggattcaaca tcgtatataa caaagacagg
     2401 gaaggtcatt cgtaagacct ctattgatga attgccacaa ttattgaatg ttttaaaagg
     2461 agaaatgtca attgtaggtc ctagaccagc gctttataat caatacgaat taatcgaaaa
     2521 acgtacaaaa gcgaacgtgc atacgattag accaggtgtg acaggactag ctcaagtgat
     2581 ggggagagat gatatcactg atgatcaaaa agtagcgtat gatcattatt acttaacaca
     2641 tcaatctatg atgcttgata tgtatatcat atataaaaca attaaaaata tcgttacttc
     2701 agaaggtgtg catcactaat gagaaaaaat attttaatta caggcgtaca tggatatatc
     2761 ggtaatgctt taaaagataa gcttattgaa caaggacatc aagtagatca aattaatgtt
     2821 aggaatcaat tatggaagtc gacctcgttc aaagattatg atgttttaat tcatacagca
     2881 gctttggttc acaacaattc acctcaagca aggctatctg attatatgca agtgaatatg
     2941 ttgctgacga aacaattggc acaaaaggct aaagctgaag acgttaaaca atttattttt
     3001 atgagtacta tggcagttta tggaaaagaa ggtcatgttg gtaaatcaga tcaagttgat
     3061 acacaaacac caatgaaccc tacgaccaac tatggtattt ccaaaaagtt cgctgaacaa
     3121 gcattacaag aattgattag tgattcgttt aaagtagcaa ttgtgagacc accaatgatt
     3181 tatggtgcac attgcccagg aaatttccaa cggttaatgc aattgtcaaa gcgattgcca
     3241 atcattccca atattaacaa tcagcgcagt gcattatata ttaaacatct gacagcattt
     3301 attgatcaat taatatcatt agaagtgaca ggtgtgtacc atcctcaaga tagtttttac
     3361 tttgatacat cgtcagtaat gtatgaaata cgtcgccaat cacatcgtaa aacggtattg
     3421 atcaacatgc cttcaatgct aaataagtat tttaataagt tgtcggtctt tagaaaatta
     3481 ttcggcaatt taatatacag caatacgtta tatgaaaata ataatgcact tgaaattatt
     3541 cctggaaaaa tgtcacttgt tattgcggac atcatggatg aaacgacaac caaagataag
     3601 gcataagtca tctattaaat aaaatcaaca tacaaatcgt tttatttgga ggttatagta
     3661 tgaagttaac agtagttggc ttaggttata ttggtttacc aacatcaatt atgtttgcaa
     3721 aacatggcgt cgatgtgctt ggtgttgata ttaatcagca aacgattgat aagttacaaa
     3781 gtggtcaaat tagtattgaa gaacctggat tacaagaggt ttatgaagag gtactgtcat
     3841 cgggaaaatt gaaggtatct acaacgccag atgcatctga tgtttttatc attgccgttc
     3901 cgacgccgaa taatgatgat cagtaccggt catgtgacat ttcgctagtt atgcgtgcat
     3961 tagatagtat tttatcattt ttagaaaaag gaaataccat tattgtagag tcgacaattg
     4021 cgcctaaaac gatggatgat tttgtaaaac cagtcattga aaatttaggg tttacaatag
     4081 gtgaagatat ttatttagtg cattgtccag aacgtgtact gccaggaaaa attttagaag
     4141 aattagttca taacaatcgt atcattggcg gtgtgactga agcttgtatt gaagcgggta
     4201 aacgtgtcta tcgcacattc gttcagggag aaatgattga aacagatgca cgtactgctg
     4261 aaatgagtaa gctaatggaa aacacatata gagacgtgaa cattgcttta gctaatgaat
     4321 taacaaaaat ttgcaataac ttaaatatta atgtattaga tgtgattgaa atggcaaaca
     4381 aacatccgcg tgttaacatc catcagcctg gtccaggtgt aggcggtcat tgtttagctg
     4441 ttgatccgta ctttattatt gctaaagacc ctgaaaatgc aaagttaatt caaactggac
     4501 gtgaaattaa taattcaatg ccggcctatg ttgttgatac aacgaagcaa atcatcaaag
     4561 tgttgagcgg gaataaagtc acagtatttg gtttaactta taaaggtgat gttgatgata
     4621 taagagaatc accagcattt gatatttatg agctattaaa tcaagaacca gacatagaag
     4681 tatgtgctta tgatccacat gttgaattag attttgtgga acatgatatg tcacatgctg
     4741 tcaaagacgc atcgctagta ttgattttaa gtgaccactc agaatttaaa aatttatcgg
     4801 acagtcattt tgataaaatg aagcataaag tgatttttga tacaaaaaat gttgtgaaat
     4861 catcatttga agatgtatcg tattataatt atggcaatat atttaatttt atcgacaaat
     4921 aaaatgtgtc aaactagggc atacatgatt aaggaaagat aagctgtcat gtgtttgaac
     4981 ttcagagagg ataatgttat gaaaaaaatt atggttattt tcggtacgag acccgaagca
     5041 ataaaaatgg caccattagt aaaagaaatt gatcataatg ggaactttga agcgaacatt
     5101 gtgattacag cacaacatag agatatgtta gatagtgtgt taagtatatt tgatattcaa
     5161 gctgatcatg atttaaatat tatgcaagat caacaaacat tagcaggcct tacggcgaat
     5221 gcacttgcta aacttgatag catcattaat gaggaacaac cggatatgat tttagtacat
     5281 ggtgatacta caacgacttt tgtaggaagt ttggcagcat tttatcatca aattccggtc
     5341 ggacatgtag aagctggact tcgaacacat cagaaatact caccatttcc tgaagagtta
     5401 aatcgagtca tggtaagtaa tattgctgaa ttgaattttg cgccaacagt aattgcagct
     5461 aaaaatttac tttttgaaaa caaagacaaa gagcgtatct ttattactgg aaatacagtt
     5521 attgacgcat tgtcaacaac agttcaaaat gattttgttt caacgattat taataaacat
     5581 aaaggcaaga aagttgtttt actaacagcg catcgtcgtg aaaatattgg ggaaccgatg
     5641 catcagattt ttaaagcagt aagagatttg gcagatgaat ataaagatgt tgtcttcatt
     5701 tatccaatgc atcgtaatcc aaaggtaaga gcgattgccg aaaaatattt atctgggaga
     5761 aatcggattg aattaattga gccattagat gcgattgagt tccataattt tacaaatcaa
     5821 tcgtacctcg tgctgacaga ttctggtggt attcaagagg aggctcctac atttggaaaa
     5881 cctgtgttgg tattaaggaa tcatacagag cgtcccgaag gcgttgaggc gggaacatcg
     5941 agagtaattg gcacagatta tgacaatatt gttcgaaatg tgaaacaatt gattgaggat
     6001 gatgaagcgt atcaacgtat gagtcaagcg aataatccat atggtgatgg acaagcatca
     6061 cgacgtattt gtgaagcaat agaatattat tttggattgc gcacagacaa gccggatgaa
     6121 ttcgtacctt tacgtcacaa ataataaaaa acccctaatc atgaagttgg tttagacaac
     6181 cagcggtgac taggggtttt taatatattt atttttgata gtggtagcca atatcatatt
     6241 tgaatacttt atttgataat attggacttt gctgtccatc gtcatcactt tttaaacgta
     6301 catttttatg agcttcttta aatacatcgg aattcaacca attattaaag ctatcttcag
     6361 attcccaaat agttaagatt ttaacttcgt ctgtatcctc ggtatttaat gttttagtga
     6421 caaacatttg ttggaagcct tcaatagttt caataccttg tctattgtaa aaacgttcaa
     6481 tcgtttcttc cgcactgcct ttttgtaatt gtaatctatt ttctgccata aacatgggca
     6541 atcactcctc tattttatga tttgatttgg gtaatgtttt tacaaatgta aagagtacag
     6601 cggtttgtat gataaccatt atgattaatc ctacacggac tgcaagaaca tccaccatat
     6661 aaattgaaaa acctattaca atgtataagc taattaaaat tttaattttc tgttgtagcg
     6721 tgtagcctcg atgtaaataa aagttttcta catattcttt ataaattttt tgattaataa
     6781 gccaattgta aaagcgatct gaacttcgag caaaacaaaa aactgctacg agtaaaaaag
     6841 gggtcgttgg cagtaaaggt aatacggcac ctgcaatacc aagcgctgta aatattaagc
     6901 caatgacgat taaaataagt cgcattgaaa aaactccatt ctagtactaa tgcgcatgta
     6961 atattgtttt agtaatataa ctcatgctaa atataatgtg tatgataagt gcaatgactc
     7021 agtaaaatga aacgatgttg aattatcctt gtcacattaa cgcattttaa gcgcgacttt
     7081 cataacaacc aaactattta atgagaatta t
//
LOCUS       DANJFZ010000125         6970 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.125, whole genome shotgun sequence.
ACCESSION   DANJFZ010000125 DANJFZ010000000
VERSION     DANJFZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.125"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            67..1065
                     /gene="argF"
                     /locus_tag="PWN45_002351"
     CDS             67..1065
                     /gene="argF"
                     /locus_tag="PWN45_002351"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0624095.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1085..2014
                     /gene="arcC"
                     /locus_tag="PWN45_002352"
     CDS             1085..2014
                     /gene="arcC"
                     /locus_tag="PWN45_002352"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0624096.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(2114..3114)
                     /locus_tag="PWN45_002353"
                     /pseudo
     CDS             complement(2114..3114)
                     /locus_tag="PWN45_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3146..3315
                     /locus_tag="PWN45_002354"
                     /pseudo
     CDS             3146..3315
                     /locus_tag="PWN45_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            3922..4272
                     /locus_tag="PWN45_002355"
     CDS             3922..4272
                     /locus_tag="PWN45_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624097.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            4359..4670
                     /locus_tag="PWN45_002356"
     CDS             4359..4670
                     /locus_tag="PWN45_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624098.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            4686..5189
                     /locus_tag="PWN45_002357"
     CDS             4686..5189
                     /locus_tag="PWN45_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL0624099.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            5203..5427
                     /locus_tag="PWN45_002358"
     CDS             5203..5427
                     /locus_tag="PWN45_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624100.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
     gene            complement(5682..6809)
                     /locus_tag="PWN45_002359"
     CDS             complement(5682..6809)
                     /locus_tag="PWN45_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0624101.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT         2439 a         1173 c         1114 g         2244 t
ORIGIN      
        1 ataaaagaaa ttggattata aaaaaataac atgaataaaa tattaattga aaaggagata
       61 ttcaaaatga aaaatttaag aaatagaaac tttttaactt tgttagactt cacacaaaaa
      121 gaaatggaat ttttacttaa tttatctgaa gatcttaaac gcgcaaaata tgcaggaata
      181 gaacaacaaa aaatgaaagg taaaaatatc gctctacttt ttgaaaaaga ttcaacacgc
      241 actcgatgtg catttgaaac agcggcttat gatcaaggtg cacatgtaac ataccttggg
      301 ccaacaggtt ctcaaatggg taaaaaagag tctaccaaag atactgctcg tgttttaggt
      361 ggaatgtatg atggcataga atatcgtgga ttctcacaaa gagtagttga ggatttggca
      421 aaatattctg gcgttcccgt atggaatggt ttaacagatg aagatcatcc tacacaagta
      481 cttgctgatt ttttaacagc taaagaagta ttgaaaaaac catataatga aattaacttc
      541 acatatgttg gagatggacg taataacgtt gcaaatgcct taatgcaagg agcagcaatc
      601 atgggcatga ctttccatct tgtatgtcct aaagaactaa atccaactga tgaattatta
      661 aatcggtgta atgatatagc tgacaaaaac ggtggtgaaa tccttataac tgatgatatt
      721 gatgaaggtg tcaaaggatc tgatgtaatt tacacagatg tttgggtatc tatgggtgaa
      781 cctgatgaag tttgggaaaa acgtatcaaa ttattagaac cataccgtgt aactaaagaa
      841 ttaatgaaaa aaacaggtaa tccacataca atttttgaac attgcttacc atcattccat
      901 gatactgaaa caataatcgg taaacaaatt caagaaaaat atggtctaac tgaaatggaa
      961 gtaacaaatg aagtatttga aagtgaacaa tcagttgttt tccaagaagc agaaaataga
     1021 gcacatacaa taaaagcagt catggtagca acattaggag agtaaatgaa aggaggatat
     1081 aaatatgtct aaaatcgtcg tagctttggg tggtaacgct ttaggacaat cgcctaaaga
     1141 acaattagat ttattaaaaa gtacatctaa atctctagta agtttaatcg ataaaggata
     1201 cgaaattgta attagtcatg gtaatggtcc acaagttgga agtattaact taggtttaaa
     1261 ttatgcggcg gaacacaaac aaggtcctcc ttttcctttc cctgaatgtg gtgctatgag
     1321 tcaagcttat attggctatc agatgcaaga aagtttacaa aacgaacttc attcaatggg
     1381 catagataaa caagttgtta cgctagtaac acaagttcaa gttgcaagcg atgattctgc
     1441 tttcaataac cctactaaac caattggatt attttataca aaagagcagg ctgataaatt
     1501 tacgaaagaa aaaggttata cttttgtaga agattctgga cgcggctatc gtcgcgttgt
     1561 cccttcccca caacctataa gtatagttga gttagatagt atagaaacat taatcactca
     1621 tggaacacta gttatcgctg ccggcggtgg tggaattcca gtaattaagg aaaatgaagt
     1681 ttatacaggt gttgatgcag ttattgataa agataaaacg agtgctttat tagcagcaca
     1741 tttacaatct gatcaattaa tcatattaac tgctgtggac catgtttaca ttaactatgg
     1801 aaaagaaaac caaagaggtc tcgatgaagt gtctgtggat gaaatgaaaa aacatatctc
     1861 tgatggtcaa tttgctaaag gaagtatgct tccaaaagtt gaagctgcac ttcaatttct
     1921 tgaaaaaaat actaaaggca gtgttttgat tacatctcta gcaggattag gggacgcttt
     1981 agacggtaaa ataggaacat taattaagaa ttgaaattat aatactttag ttcaattacc
     2041 tttataaaca aaagagccta taatcctagg gcactgaacc cttaaaacgg gaacttaata
     2101 aaaacaccat gttctaggct attaattctc tgtattttac aggggataag tagcctagtt
     2161 tttgttgaat tcgattatta ttattattat tattgttttt aatgtacttt tcgacaatat
     2221 ctattacaat gtgattagag tgattaagct cattattgat gtaaaaagtt tcagacttta
     2281 gcgaggaatg gaaactttct atcggggcgt tatcggcagg tgttcccttt cgggacatac
     2341 ttctgataat gcctttttct tcgcacaatt gataataagc ataagatgta taaacgctgc
     2401 cttgatcact atgtaatata caaccctcag gtatatcgat ttgatttaat gtatcattaa
     2461 ctaaactttg gtcttgttta tcatctattt tatacgccac aatttctccg ttataaatat
     2521 ccattatcga agataaatac aacatagaat gaccaaatgg taaataagta atatcggttg
     2581 ttaatacttc cattggacaa ctcgctttaa attgtctttg taataaattg tccgttttat
     2641 aatacggttt acctattttt gtcatctttt taggtctaac tcggcagttc aaatgatgct
     2701 tctgcatcat tctctgtact ctcttatgat taattggtga tgtataacat tgattaatca
     2761 atgctgtaat cttacgataa ccgtaggtat agtggttagc tttacataat tcaataactt
     2821 tttgtgttac ggtatcattt ttatgggttt tgtttttcca tcggtaatat gttgatttag
     2881 gtatgtttaa tacttttagt agcaatttga ttgaatattt tacttttaat tgatccacta
     2941 aatctatgac tactgttggt accacttcct ttccaatacc ttgtactttt ttaaaatatc
     3001 caattctata tcttttctct tattttctaa ttttaattgt tctacttctg acagctcttc
     3061 taatccttta ccgtaggtat attgtttacc aacttgttgt gaaaatctat atgctagttt
     3121 tggtaaaact gaggaaggac gctggatagc taagaatgcc cataattatg gatttattat
     3181 aagatatcct aaaaacaaag aaagcatcac aggatatcaa tatgaacctt ggcatttacg
     3241 ttatttagga aaaagaaggc gacaaaagtg tacaaatctg gtgaaacatt agagggattt
     3301 gtaggcttaa aatgatcaat ttaaaataat cgaaacttca actaccttat tatcgacgaa
     3361 gtgtattcca gcacttcgtc tttattttta ccgcaaacat tataccttac tatttaataa
     3421 atctatttta tatctacata cttcctcttt ctttttctaa aaatttttga gaattctctt
     3481 tatcaatttc aataacactt atctaatgaa acagtcattg tcgtaatttt ttcataacta
     3541 cacgaggctt atattgaaat tgctaaagaa catcccacga tgatttttta tcgggaaagg
     3601 aatgggattt gcagattaga agaagcagaa ggattgtgca agcacaatcc caaaatcccg
     3661 ttattcttta taaataaata atatatataa ggaagcagag accccaccct atcatcaata
     3721 cttataaata atgagttgat tgttcgtcaa aataggcgga atttcgggat ttccttgtca
     3781 taccaaggtt tgtagcggtt ttttatatgg aattttctca tattgtcaac gggatcagta
     3841 ataaatgaca aatcaaatat atattatctc aatgatgtat atgaagtaca tcactatcac
     3901 actatctagg gaggcaatga tatgaaaaca actactcaag aactcaaaca atatatgact
     3961 cgcttattcc aattatctaa caatgaaaca tgggaatgtg aaacactgga ggaagcggca
     4021 gaaaatattc tacccaaacg ttttattaat gattccccac ttgcacatct tatacttgaa
     4081 acttatacct actataataa tgagctacat gaactaagta tctatccatt tctaatgtat
     4141 tctaataacc aactcatcag tattggttat ctggatcatt ttgatatgga ctttctatac
     4201 ctcacagata ctaaaaatac gattatcgat gaacgtcatt tactaaaaga aggagggaat
     4261 aaccatgaat aattggatca aagtggcaca aatatctgtt acagtcatta acgaagtgat
     4321 tgacatcatg aaagaaaagc aaaatggagg aaaatagtat gaaaatcaat cgatacatca
     4381 caagaggcat tagtgaacaa ctatctctag accttcaaat cttactttgg aacatggtaa
     4441 aagatcgaga caatcaactt aatacagatt acctacacat tttcaaacta caagaagatg
     4501 ataatatgtt gtcaattaca catgaacaag aacaacccgc atacaaattg gaatatcact
     4561 atacaaacta tataaaaaat caaaatgcat tacctaagaa agtctacgtc atccgagaag
     4621 atgatgtaga cgctttttat tatgtgatgc ttttgccaga agaatactaa aggaagtgac
     4681 gatatatgaa tacaatcaaa agtacgatac acacagaagc gatatttagc gatgatgaac
     4741 aacatcgcta cttactcaaa aagatatggg atgacaagaa accggcttgt actgtgataa
     4801 cgatgtatct tcatttagat ggtgtattat cactcgatct tactacggtt ctcatcctca
     4861 atcaattagc taattctgag caatatggcg ctgtttatct cgttaatctt ttctctaata
     4921 ttaaaacacc agagaacctt aaacatatca aagagcctta tgatgagcac acagatatac
     4981 acttaatgaa agcaattagt gaaagtgaca cagtcattct tgcttatggt gcctatgcga
     5041 agcgaccagt tgttatcgac cgtgtcgaac aagtgatgga aatgttaaaa cctcataaaa
     5101 agaaagtaaa aaagctcata aatccagtaa cgaacgaagt tatgcatcca ctcaacccta
     5161 aagcacgtca aaaatggaca ctaaagtaaa ggagtattat ctatgaacca tgacaacact
     5221 atacaatcca attggcgaac ggttgctagt tgtttagcat cacaaaatta catttcaata
     5281 gtaaaaggtc tggtgcatca tttcacagcc atcgatgacg aggaaatact tgataaaatc
     5341 tatgaagagt ttataaatga tgactctatt acaacggtac ttaacaatga tttacaaaca
     5401 attattaatc attatctatc aaaatgaaaa ctatattcta ataaaactag agcatccttc
     5461 acttttgcgg tgaaggatgc tcctatatct ttttttatct aaaaaaacta ttagtaatta
     5521 gttttaaaac atttatcttc taatatatag atatgaaatg taaaaataca attagattgg
     5581 cagtcacaat gcaaatgtaa tatttgtcag taatcttcct actcaaattg ttattctttt
     5641 catagaaatc aagaggagga acgacttgaa ttctattata ttcaatgcat cttcattata
     5701 attctaccta cttcaccatt agacaaacga tcaaatccct cattaatttc atctaaagtg
     5761 ataacctcac tgatgagtga atcaatattt aaacgtcctt ggttgtatag attaacgaac
     5821 cttggtatat ctctgtcagg tacacaactt cctacatatg atcctttaat ggtacgttct
     5881 tcagccgcta aagtaacttg agggaaagaa aaattatctt taggattagg cagtcctgtt
     5941 gttacagttg tcccccctcg tttagtaatc tgataagcaa ctttcatagc tggcactaca
     6001 cccgctgttt caaaagcata ttctactccg ccaggaatat attctttaat ttgctcatcg
     6061 atatctttat cgcttgaatt aaatgttgct gtagccccaa attgctttgc taattcaaat
     6121 ttatcttcgt taatatctaa agcaataatt tcattggctc ccgctagttt agctccaata
     6181 atagcattca atccaatacc tcctaaacca acaaccgcta cattactacc agaacgaatt
     6241 tgggctgtat tcaccacagc accgatacct gtgataatag cacaaccaaa tgcagctgca
     6301 cgttcaaaag gtattttttt atcaatttta actatagagt tttcagagac aacagaatat
     6361 tcagcaaaac ctgatatccc caagtgatga tatacttttc cttcatcatt agataaacgc
     6421 atccctccct ctaacatttc gcctttttca ttagatattg ctccgttttc acataatgca
     6481 ggacgtcctt ctttacatgg gatacatttt ccacaactgg gaataaatgt gcataccaca
     6541 tgatctcctt cgctaactct tgtaacagct tttccaactt taattacttc accggaagct
     6601 tcatgaccaa gtgccatagg taaaggtcta ggtctgttac cattaattac tgacaaatca
     6661 gagtgacata atccagctgc atgaatttta attaatactt cgtgttcgct aggattatca
     6721 agttctaagt attcaatttt taaaggttta gattctttat agggcttttt agcccccatt
     6781 tcatgtaaaa ctgctgctct agttttcact tccaacactc ctttaattta tatacattaa
     6841 gtctattctt atctgttatt ttacacttta atattattat ttacaaaaaa ttctgattat
     6901 tgagattatt ttatataata ccaattttta aaaatttatt atgtaaaatc ataatttaga
     6961 agtgatttgg
//
LOCUS       DANJFZ010000126         6966 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.126, whole genome shotgun sequence.
ACCESSION   DANJFZ010000126 DANJFZ010000000
VERSION     DANJFZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.126"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            343..1326
                     /locus_tag="PWN45_002360"
     CDS             343..1326
                     /locus_tag="PWN45_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL0624102.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1517..2443
                     /gene="pstC"
                     /locus_tag="PWN45_002361"
     CDS             1517..2443
                     /gene="pstC"
                     /locus_tag="PWN45_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL0624103.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2445..3362
                     /gene="pstA"
                     /locus_tag="PWN45_002362"
     CDS             2445..3362
                     /gene="pstA"
                     /locus_tag="PWN45_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL0624104.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3409..4260
                     /locus_tag="PWN45_002363"
     CDS             3409..4260
                     /locus_tag="PWN45_002363"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0624105.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4267..4908
                     /gene="phoU"
                     /locus_tag="PWN45_002364"
     CDS             4267..4908
                     /gene="phoU"
                     /locus_tag="PWN45_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL0624106.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(5048..6862)
                     /gene="pepF"
                     /locus_tag="PWN45_002365"
     CDS             complement(5048..6862)
                     /gene="pepF"
                     /locus_tag="PWN45_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL0624107.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQNPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2425 a         1092 c         1183 g         2266 t
ORIGIN      
        1 cagacgggca aagcgaagcc attcaatacg aagtattgta taaatagaga acagcagtaa
       61 gatattttct aattgaaaat tatcttactg ctgttttttt agggatttat gtcccagcca
      121 tttttgtatt catatttaaa tttcgatgat ttttcaggaa gcattttaat tttactaatg
      181 aagcaatatt ttttagatta acaaaaatta atatttacat tttcttaaca attttttatg
      241 taacatttac agtttctaaa aatgaggtta ataattcaag gttaagataa agatgtaatc
      301 aatacaaata ctatttgttg ttcatacagg gaggatattt caatgaaaaa atggcaattt
      361 gttggtacta cagctttagg tgcaacacta ttattaggtg cttgtggtgg cggtaatggt
      421 ggcagtggta atagtgattt aaaaggggaa gctaaaggtg atggctcatc aacagtagca
      481 ccaattgtgg agaaattaaa tgaaaaatgg gctcaagatc actcggatgc taaaatctca
      541 gcaggacaag ctggtacagg tgctggtttc caaaaattca ttgcaggaga tatcgacttc
      601 gctgatgctt ctagaccaat taaagatgaa gagaagcaaa aattacaaga taagaatatc
      661 aaatacaaag aattcaaaat tgcgcaagat ggtgtaacgg ttgctgtaaa taaagaaaat
      721 gattttgtag atgaattaga caaacagcaa ttaaaagcaa tttattctgg aaaagctaaa
      781 acatggaaag atgttaatag taaatggcca gataaaaaaa taaatgctgt atcaccaaac
      841 tcaagtcatg gtacttatga cttctttgaa aatgaagtaa tgaataaaga agatattaaa
      901 gcagaaaaaa atgctgatac aaatgctatc gtttcttctg taacgaaaaa caaagaggga
      961 atcggatact ttggatataa cttctacgta caaaataaag ataaattaaa agaagttaaa
     1021 atcaaagatg aaaatggtaa agcaacagag cctacgaaaa aaacaattca agataactct
     1081 tatgcattaa gtagaccatt attcatttat gtaaatgaaa aagcattgaa agataataaa
     1141 gtaatgtcag aatttatcaa attcgtctta gaagataaag gtaaagcagc tgaagaagct
     1201 ggatatgtag cagcaccaga gaaaacatac aaatcacaat tagatgattt aaaagcattt
     1261 attgataaaa atcaaaaatc agacgacaag aaatctgatg ataaaaagtc tgaagacaaa
     1321 aaataataag acgcaatttc aaatgtgtct tgaaacatga ttttgatggt gaatcattat
     1381 ttagagtaca aagcttgatt tatcgagacg ctgattttga cattcagtta gtctacaagc
     1441 ttatcaactt aaaatagtgg ttcatcatta ttttacaaat ctaattattt tgggagtaat
     1501 agaaagaggt ttgattatga cttcatctac taatgttaaa gctttaatcg aaaaaaataa
     1561 taataaaaaa ggaaagcata atgacaaaat tataccagtt attttagccg caatttcagc
     1621 gatttccatt ttaacaacac taggtatatt aatcacattg cttttagaaa ccatcacttt
     1681 tttcaccaga attccaataa ctgaatttct attttctact acttggaatc ctaccggttc
     1741 agaccctaag tttggtatct gggcattgat aatagggact ttaaaaatca cagttattgc
     1801 gactatattt gcagttccag tcgggcttgg tgcagcaatt tatttaagtg aatatgcaag
     1861 tgaccgtgca cgacgcataa ttaaaccgat attagaaatt ttagcaggaa taccaacaat
     1921 tgtgtttggt ttctttgcat taacctttgt tacaccagta ttaagatctt tcataccagg
     1981 tcttggagag tttaatgcta taagtcccgg cttagttgtc ggtattatga ttgtccctct
     2041 catcacaagt ttgagtgagg atgcaatggc atctgtacca aataaaattc gagaaggtgc
     2101 ctatggactt ggagcaacta aattagaagt agcaactaaa gtcgtacttc ccgcagcaac
     2161 atcaggtatt gtagcttcaa tcgttctcgc gatttcaaga gcaattggag aaacgatgat
     2221 tgtatcatta gcggcaggta gttcgccaac agcttcatta agtttaacaa gttcgattca
     2281 aacaatgact ggatatattg ttgagatagc gacaggtgat gcaacatttg gatcaaatat
     2341 ttattacagt atttatgctg tagggttcac actatttatc tttaccttaa tcatgaattt
     2401 actttctcag tggatttcta agcgttttag ggaggagtat taatatggaa acgacagata
     2461 ataatagaca atcactcgtc gatcaacaac ttgtccaaaa acatttatca tccagaacgg
     2521 ttaaaaataa agtgttcaaa ctcatatttt tagcatgtac attattagga cttgtcgtac
     2581 ttattgcgtt gttaactcaa acattgatta aaggggtaag tcatttaaat ttacagtttt
     2641 tcactaattt ttcttcttca acaccatcta tggctggcgt taaaggcgcg ttaatcggtt
     2701 cactttggtt aatgttaagt atcattccat tatcaatcat cctaggaata ggtacagcta
     2761 tatacttaga agaatatgcg aaaaacaaca aatttactca gtttgttaaa atcagtattt
     2821 ccaatttagc tggtgtacca tcagttgtat ttgggttatt aggttatact ttgttcgttg
     2881 gtggtgcagg gattgaagcc ttgaaaatgg gtaacagtat attggcagca gcgctaacaa
     2941 tgaccttact gatattacca attattattg tttcaagtca ggaagcaatt agagctgtac
     3001 ctaactcagt acgcgaagct tcttacggct taggtgctaa taaatggcaa acgataagac
     3061 gtgttgtctt accagcagcg ttacctggta ttttaactgg attcattttg tctctttcaa
     3121 gagcactggg agaaacagcg ccacttgtgc taatcggtat accgactata ttattggcaa
     3181 cacctagaag tatattggat caattttcag cattacctat ccaaatattt acttgggcga
     3241 aaatgcctca agaagaattc cagaatgttg catcggcagg cattatcgtt ttactagtta
     3301 tcttaatctt aatgaatggc gttgcgatta ttttacgtaa caaatttagt aaaaaattct
     3361 aatttaaaca atcaatctca tttatctatt aaaaagggag ttttaaatat ggcgcaaaca
     3421 cttgcacaaa ctaaacaaat atctcaaagt catacgtttg atgtctcaca aagtcatcat
     3481 aaaacaccag atgatacaaa ctcacattct gttatatatt caacacaaaa tttagactta
     3541 tggtatggcg aaaatcatgc attacaaaat attaatttag atatttatga aaaccaaatt
     3601 actgccatta taggtccatc tggttgtggc aaatcaacat atattaaaac tttgaatcga
     3661 atggttgagt tagtaccttc tgtgaaaaca gctggtaaaa tattatatcg agatcaagac
     3721 atttttgatc aaaaatattc taaagaacaa ttacgtacaa atgtgggcat ggtctttcaa
     3781 caacctaatc catttccaaa atcaatatac gataatatta cttacggtcc aaagattcac
     3841 ggtattaaaa ataaaaaagt tcttgatgaa atcgttgaga aatcattacg tggcgctgca
     3901 atttgggatg aattaaagga taggttgcac acaaatgcat atagtttatc cggtgggcaa
     3961 caacaacgtg tttgtatcgc gcgttgttta gcaattgaac ctgaagtcat tttaatggat
     4021 gaaccgacat cagcattaga tccaatctca acattaagag tagaagagtt ggttcaagaa
     4081 ctaaaagaaa agtatacaat tattatggtt acacataata tgcaacaagc agctcgtgta
     4141 tcagataaaa ctgcattttt cttaaatggt tatgtcaatg aatatgatga tactgataaa
     4201 attttctcta acccatcaaa caagaaaaca gaagattata tttcaggaag gtttggttga
     4261 tatataatgg caataattag acaacgatat caggagcaac ttgatgattt aataaaagaa
     4321 ttacgtcggt taggtgcaaa tgtctatgtg agtattgaaa atggtataaa atcattaagt
     4381 attgacgata gaggctttgc acgacaaaca gttaaaaacg ataaacatat caatcaatta
     4441 aattatgata ttaatgagcg agttatcatg ttaattacaa agcaacagcc cattgcgagt
     4501 gatttgcgta tgatgatttc ttcattaaaa atcgcctccg atttagaaag aataggagat
     4561 aatgcctcga gtattgccaa tattcgattg cgtacaaaga ttacagatga ttatgtgtta
     4621 acccgtttaa agacaatggg taaattagct atgttaatgt taaaggactt agatcaagca
     4681 tttaaaaaga aagataccgt attaataaga gaaataattg agcgtgatga agatatcgat
     4741 gacttatata gtcatattat taacgcaacg tatcttattg ataacgatcc atttgtcgct
     4801 gcacaagctc atttagcagc aagacattta gaacgtattg gtgatcatat tattaacatc
     4861 gctgaaagtg tttattttta tttaacaggt acacattacg aacaataact taaagttatt
     4921 actataaaat cccttacgat aaatatatat ttctattatt cataaaccct caaaaaaacc
     4981 aagattctca caattagtaa tgtgaaaatc ttggtttata ttgttctact ataaattgtc
     5041 tcgcatctta gttatttgct tgctcaattt catctgttaa tttttcaact tcatcgacta
     5101 aatcagaaat atattgaatt gtagatttaa gtggctgttc tgtagtaatg tctacacctg
     5161 caatgtttgc aagttcgaca ggtgatacac taccaccttt tttcaatgtt tctaaccaag
     5221 catcaacagc tggttggcct tcatttttaa tcttttgaga aacgacagtt ccgattgtta
     5281 agccagcaga atacgtatac gaatataatc ccatatagta atgaggttga cgcatccatg
     5341 ttaattcagc accctcagtc atgtctactg catctccaaa aaattgttta taaacattta
     5401 gcattatttc atttaatgtc ggcgcattta aagattcacc ttgatctact ttgtgataca
     5461 cttcacgttg ataagcagct tctaataaat gggtaaccat attatgataa tatgttctag
     5521 ataaaattga gccaataacc caacgcttaa atcttggatt atcacttgtg ttaaataaat
     5581 aattggccat caacatttca ttcattgtag aaggggcttc aacaaagtac attgatgctt
     5641 ctgattcaag atatggttga tgtttttgag ctaatgtaaa atgacctgca tgacctaatt
     5701 catgtgctaa gacaaatgct tcagccattt taccagtcca agaaataaac acatatgaat
     5761 gtgtaaagta tggacttgca caaaatgcgc ctgtatcttt acctttattt tgtgcaaaat
     5821 caatccatcg ctgatcgtat gcttcacgta acatgtttgt atagtcatca cctaaaacac
     5881 ttaacgcacc gaaaatataa ttttttgagt cttcaattga aatctctggt tcataatcag
     5941 ggtctacaga aatcttcaag tcttcaaaac gcatgttatc taatccgtga atacgttgta
     6001 aaagtttagc atatttctgc ataactggtg ccaagtcacg cataatcata tcgatttgac
     6061 ggtcaaacat atcacgcgtt acttcttgac tatgtaataa ataatcgatg actgattcaa
     6121 atccacgtaa atcagcttca attttttctt gttgtacttg catattatat gtagccgcgg
     6181 tagtatgctg atattttcga atcccatcgc taaacgattt gaaactttta cgtctaaact
     6241 caggatcttt attatcttca taatcatttt caaacgtagc atagtcgaca gggtacgttg
     6301 taccatcatg ttcaaatgaa tcgaatgtaa tatctagcat ttttgtcgtg ccgtataaat
     6361 cgtaaggact gtttagcgta ggtgataaag ttgctaatac ttgttctacc gacgcagata
     6421 attggaatgg attttgtttt attaactgtt taatatagtg ttgatatgga catgattctt
     6481 ctaattgttg aagtatttct tctggtaatt caagtatttc tgactctaca aatgataatt
     6541 ggctaacaat tttaccgtat gtagtagata atttagcgct caatacttgt gcctcgatat
     6601 tactagtatc tacacttaaa cgtagttctg cataattact taagcgatct aaggcaatta
     6661 aaatattttc taattcagct aaagcagtat taatttgttc gattgaattt aatgttgttg
     6721 catatgtatg atgaaattga tttgcttgtt gtactagagc gtcaatactt tcataatatt
     6781 gttgatcatc tttaaataag tctactaaat cccatgtttc tgaaacagga acatcttctc
     6841 ttaaaggtaa accttgagac atataatcac tcctttggta taatacattc attatacata
     6901 attttgtctc ctaaacgata atgttacgat ttccatgtcg caaggaacaa tgatttttct
     6961 attagt
//
LOCUS       DANJFZ010000127         6894 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.127, whole genome shotgun sequence.
ACCESSION   DANJFZ010000127 DANJFZ010000000
VERSION     DANJFZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.127"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..673
                     /locus_tag="PWN45_002366"
     CDS             <1..673
                     /locus_tag="PWN45_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDL0624108.1"
                     /translation="AIEYSDLHHKSKFDSKRLSNAKMSFINPTQLENKNTNDRLLKHD
                     LLFHDMFVNDDWKKDFKVEFENEALSKKFINKDIDIFAGNYGYGCHGGATNKTQCSYG
                     GVTLSDNNKYDDYKNIPCNLWIDGHQTEIELTAVKTKKKIVTIQELEVQLRNYLNEKY
                     KLYEQGGDIVKGYVKYYNDDEQNVEYDFYNLNGEYGREVLKMYADNKTINSDKLHLDI
                     YLFKS"
     gene            complement(875..1144)
                     /locus_tag="PWN45_002367"
     CDS             complement(875..1144)
                     /locus_tag="PWN45_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624109.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1458..2144
                     /gene="mtnN"
                     /locus_tag="PWN45_002368"
     CDS             1458..2144
                     /gene="mtnN"
                     /locus_tag="PWN45_002368"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL0624110.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2164..2691
                     /locus_tag="PWN45_002369"
     CDS             2164..2691
                     /locus_tag="PWN45_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL0624111.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            2692..3792
                     /gene="yqeH"
                     /locus_tag="PWN45_002370"
     CDS             2692..3792
                     /gene="yqeH"
                     /locus_tag="PWN45_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL0624112.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3806..4612
                     /gene="aroE"
                     /locus_tag="PWN45_002371"
     CDS             3806..4612
                     /gene="aroE"
                     /locus_tag="PWN45_002371"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL0624113.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4616..4906
                     /gene="yhbY"
                     /locus_tag="PWN45_002372"
     CDS             4616..4906
                     /gene="yhbY"
                     /locus_tag="PWN45_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL0624114.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            4909..5478
                     /gene="nadD"
                     /locus_tag="PWN45_002373"
     CDS             4909..5478
                     /gene="nadD"
                     /locus_tag="PWN45_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDL0624115.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5468..6052
                     /gene="yqeK"
                     /locus_tag="PWN45_002374"
     CDS             5468..6052
                     /gene="yqeK"
                     /locus_tag="PWN45_002374"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL0624116.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6053..6406
                     /gene="rsfS"
                     /locus_tag="PWN45_002375"
     CDS             6053..6406
                     /gene="rsfS"
                     /locus_tag="PWN45_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL0624117.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            6409..>6894
                     /locus_tag="PWN45_002376"
     CDS             6409..>6894
                     /locus_tag="PWN45_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0624118.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AV"
BASE COUNT         2575 a          847 c         1317 g         2155 t
ORIGIN      
        1 tgcaatcgaa tattcagact tacatcataa aagtaagttt gattcaaagc gtctaagtaa
       61 tgctaagatg tcattcatca atccaactca gcttgaaaat aaaaacacaa acgatagact
      121 gttgaagcat gatttgttat ttcatgacat gttcgtaaat gatgattgga aaaaggattt
      181 taaagttgaa tttgaaaatg aggcactttc aaagaaattt ataaataagg atatcgatat
      241 atttgctgga aattatggat acggatgtca tgggggagca accaataaaa cgcaatgtag
      301 ttatggtggt gttactttaa gtgacaataa taaatacgat gattataaga atataccttg
      361 taatttatgg attgacggac atcaaacaga aatagaacta actgcagtaa aaacgaaaaa
      421 gaaaattgtt actattcaag aattagaggt tcaattaaga aattatttga atgagaagta
      481 taagttgtac gaacaaggtg gcgacattgt taaagggtat gttaaatatt ataatgatga
      541 tgaacaaaat gtagaatatg atttttataa tttaaatggt gagtatggtc gtgaggtatt
      601 aaaaatgtat gctgataata aaactatcaa tagtgacaaa ttgcatttag atatctattt
      661 attcaaatca taatttaatt aagttaatca ttaataatct ttgacagtga aatataaagt
      721 acctgtcatt atgttgttac aaataaaaac agcagtaagg atgctaacac ttagaaaagt
      781 cttactgctg ttttctatta gaattatctt taatgcacaa aggaattcga tacgaaacaa
      841 atatgattaa ctgtgtaatt acaaaataaa actactatag taaaaagaag ataaagacga
      901 tgataataaa tgcatcgaat ataattttct tgatgttttt atcaacaaca tcaggtaagg
      961 acataagtat aaatcctaga attagagtgt agcgacatat gttcataatt gttgctgtaa
     1021 actcgatatt aaatgaaaac tttaaaaata tacttataaa taaaatgaca aacgacgcaa
     1081 ttgcaataaa actgccgtat gtagctatct ttgttacgag actattttga ttattttcag
     1141 ccattgtaaa atcctcttat ttcatcatta ttattttaaa catatcattt tcctgttatt
     1201 taataaagtt tcttgacacg attggttgat gtaattgata tgattagttt ttgaaaaata
     1261 gacaaattag aagcatcgaa ttcactgtta tgagttcaaa aataattatt tatgatacca
     1321 agttattgaa agagcttttt ataaaaataa agcaaaagtt ttatacagta atgaaaatca
     1381 tgatacttgt cgttgtagtg attaaaatgt gataaaataa caaatgctag gattttttaa
     1441 aagaggtgaa tcaaaagatg attggtataa ttggtgccat ggaagaagaa gtaacaatat
     1501 taaaaaataa attaacacaa ttaagcgaaa tttcagttgc acatgttaaa ttttatactg
     1561 gcattttaaa agatagagaa gtagtgatta cccaaagtgg cattggaaaa gttaatgctg
     1621 caatttctac gacattatta attaataagt ttaaaccgga cgtcattatt aatacaggtt
     1681 ctgctggagc tttagatgaa agtttaaatg taggtgacgt tcttataagt gatgatgtaa
     1741 aatatcatga tgcagacgca acagcatttg gttatgaata tggacaaata ccacagatgc
     1801 cggtagcatt tcaatcaagt aaacctttaa tagaaaaagt atctcaagtt gtacaacaac
     1861 aacaattaac agctaaagta ggcttaattg taagtggtga tagctttatc ggtagtgttg
     1921 aacaacgcca aaaaattaaa aaagcatttc caaatgcgat ggcggttgaa atggaagcaa
     1981 ctgcaattgc acaaacatgt tatcaattta atgtaccatt tgttgtagtt cgtgcagttt
     2041 cagacttagc aaatggagaa gcggaaatga gcttcgaagc atttttagaa aaagcagctg
     2101 tatcatcaag tcaaactgtt gaagcattag tgtctcaatt ataaaaaggt aaaggtgagt
     2161 taaatgggtt tagttcgcaa gttttttatg ccgaattcat atgttcaatc aatatttcaa
     2221 attgatttag acaagttagt ggacaaaggc gttaaaggta ttattacaga tttagataat
     2281 acgctagtag gttgggatgt taaagaacct acagaacgtg ttaaagcatg gtttaaggaa
     2341 gctaatgaaa aaggaatcac tattacaatc gtgtctaata ataatgagtc tcgtgttgct
     2401 agttttagtc agcatttaga catcgatttt atttttaaag cgagaaagcc aatggggaaa
     2461 gcgtttgata aagcaataac taagatgaat atcagaccag atcaaactgt tgttataggt
     2521 gaccaaatgc ttactgatgt atttggtggt aatcgtcgag gtctatatac aattatggtt
     2581 gttccagtta aacgaactga tggctttatt actaagttta atagattaat tgaaaggcga
     2641 ttattacgtc atttcagtaa aaaaggttat atcacatggg aggaaaattg attgtctgac
     2701 attttaaaat gtatcggttg tggtgcgcca cttcaatctg aagataaaaa taaacctggt
     2761 tttgtaccag agcataatat gtttcgtgat gacgtgattt gcagacgttg tttccgcttg
     2821 aaaaattata acgaagttca agatgtagga ttagaaagtg aagacttttt aaaattatta
     2881 tcaggacttg cggataaaaa gggtattgtc gtcaatgtcg tggatgtatt tgactttgaa
     2941 ggatcattta ttaatgcagt taaacgtatt gtcggaaaca aaaaaatcat tttagcagca
     3001 aataaattgg atttattgcc gaaacaaatt aataaacgtc gagttaaaga atggttaaaa
     3061 cgaacagcaa gaaaatatgg tttggaagct gacgatgtcg tattaatttc agctgaaaaa
     3121 ggctggggca tagacgactt attatcatca attgcgaata ttcgagaaaa tgaagatgtg
     3181 tatattgtag ggacaacgaa tgttgggaaa tctacattga ttaataaact gattgaagct
     3241 agtgttggtg aaaaagatgt agtaacaact tcaagattcc ctggaacaac tttagatatg
     3301 atagatattc ctttagatga aacatcattt atgtatgata caccaggtat tattcaagat
     3361 caccaaatga cgcatttagt tagtgaaaaa gaattgaaaa ttattatgcc taagaaagaa
     3421 ataaaacaac gcgtatatca attaaatgag gcgcagacat tattcttcgg cggtctagcg
     3481 cgcatagatt atgtatcagg tggtaaacgt ccgttagttt gtttcttttc taatgacttg
     3541 aatatacatc gtactaaaac ggagaaggct aatgatttat ggcgtaatca acttggcgat
     3601 ttattaacgc cacctggaaa tccacaaaat tttgatctta atgaggtaaa ggctgttaga
     3661 cttgaaacag gcaaagagaa acgcgatgtt atgatctctg gtctaggctt tataactata
     3721 ggaccagggg ctaaagtaat cgttcgtgtt cctaaaaatg ttgatgttgt attaagaaat
     3781 tctattttat aaggtgatta aaaaaatgaa atttgcagtt ataggaaatc ctatttcaca
     3841 ttccttgtcg cccgttatgc atagagcaaa ttttaattct ttaggattag atgatactta
     3901 tgaagcttta aatattccaa ttgaagattt tcatttaatt aaagaaatta tttcgaaaaa
     3961 agaattagaa ggctttaata tcacaattcc tcataaagaa cgtatcatac cgtatttaga
     4021 ttatgttgat gaacaagcga ttaatgcagg tgcagttaac actgttttga taaaagatgg
     4081 caagtggata gggtataata cagatggtat tggttatgtt aaaggattgc acagcgttta
     4141 tccagattta gaaaatgcat acattttaat tttgggcgca ggtggtgcaa gtaaaggtat
     4201 tgcttatgaa ttagcaaaat ttgtaaagcc caaattaact gttgcgaata gaacgatggc
     4261 tcgttttgaa tcttggaatt taaatataaa ccaaatttca ttagcagatg ctgaaaagta
     4321 tttagctgaa ttcgatatcg ttattaatac aacaccagcg ggtatggctg gaaataacga
     4381 aagtattatt aatttaaagc atctttctcc caatacttta atgagtgata ttgtttatat
     4441 accgtataaa acacctattt tagaggaagc agagcgcaag ggaaaccata tttataatgg
     4501 cttagatatg tttgtttacc agggtgcgga aagctttaaa atttggacta ataaagatgc
     4561 tgatattaat tctatgaaaa cagcagtttt acaacaatta aaaggagaat aacatatgct
     4621 tactggcaaa caaaaaagat acttaagaag tttagcacac aatattgatc cgatttttca
     4681 aattggaaaa ggcggtatca acgaaaatat gattaaacaa atagatgata cgttagaaaa
     4741 cagagaattg attaaagtac atgtactaca aaataacttt gatgataaaa aagaattagc
     4801 tgaaacatta agcgaagcta ctcatagtga attagtgcaa gtgattggat ctatgatagt
     4861 gatttataga gaatctaaag ataataaaga aattgaattg ccataataat gaaaaagata
     4921 gtactttacg gcggtcagtt taaccctatc catactgcac atatgatagt agctagcgaa
     4981 gtatttcatg aattacagcc agatgaattt tattttttac ctagttttat gtctccattg
     5041 aaaaagcacc atgattttat agacgttcag cacagattaa caatgataca gatgattatc
     5101 gacgagcttg gttttggaga tatttgtgac gatgaaatta aacgtggtgg tcaaagttat
     5161 acctatgaca cgatcaaggc attcaaggag caacacaaag acagtgagtt gtactttgtt
     5221 attgggacgg atcagtataa ccaactagag aaatggtatc aaattgaata cttaaaagaa
     5281 atggttactt ttgtagttgt aaatcgagac aaaaatagtc aaaatgttga aaatgctatg
     5341 attgcaattc agatacctag ggtagatata agttcgacaa tgattcgaca aagagttagt
     5401 gaagggaaat ctatccaagt tcttgttcct aaatccgttg aaaactatat taagggggaa
     5461 ggattatatg aacattgaaa aagcaaaacg gcttgcaaaa gagaaactgc cagagaaacg
     5521 ttataatcat tctttaagag ttgcagaaac agcgattaaa ttggcagaaa tttatgatgg
     5581 agacactagt aaagtagaat tagcaggtgt attacatgat ttctgtaaat atgatgattt
     5641 aggtaaaatg tatcaaattg ttcgacaata cgaattaggt aatgatctac tgagttatgg
     5701 tagtgaaata ttgcatggcc ctgtgtgtgc agcaatcatg gaacatgaat atggtatcaa
     5761 tgatgaggaa gtattaatgg ctatcaaata ccatactact ggacgtcaac aaatgacaaa
     5821 aactgaaaaa ctgattttta ttgcagatta catcgaacct ggaagaacaa tcccaggagt
     5881 tgatgatatt cgagatatgg catacaatca aggtagttta gataagacaa tttatgaaat
     5941 ttctaaacgc acagtactat ttttaataca gaaagatatt acggtatata ataagacgat
     6001 tgactgttta aattattata actatagtga cgaaagaata aaggatgatt aaatgaattc
     6061 acaagaatta ttagcaattg ctgtggatgc aattgacaat aaaaaaggcg aagatacgat
     6121 ttctttagaa atgaaaggta tcagcgatat gacagattat tttgttgtaa cgcacggaaa
     6181 taatgaacga caagttcaag cgattgctag agcggtgaaa gaagtagcca atgaacaaaa
     6241 tatagaagta aaacgtatgg aaggatacaa tgaagcgcgt tggatattaa ttgacttagc
     6301 tgatgttgtg gtacatgttt tccataaaga cgaaagaaat tattataata ttgaaaagtt
     6361 atatcaagat gcaccattag aatcatatag tcaggttgcg tattaattat gtcgcaatat
     6421 gcagaaatga gcctagtgta cgatcaattg actcaagatc aaccatatga aaaatggttt
     6481 gaaattgtaa aaaatcactg caaagatgaa tcaaatattt tagatattgg atgcggtact
     6541 ggtagtttaa cagttcaatt agaagcttta ggtaatgtta ctggtatgga tttaagtgtt
     6601 gatatgttga ctgttgcagc tcaaaaatca gcaaatgtaa attggcttga aggagatatg
     6661 acatcttttg atttgcaaca acaatttgat atcatcacta ttttttgtga ctctctaaat
     6721 tatttgcaag atgaaactgc agtgattgaa acattcatca atgtttatca tcatctgact
     6781 gattcaggtg tatttatttt tgatgtacat actgtttata aaatgatgac tttgtttaat
     6841 aatcaaagtt atattgatga taaaggggac atttttttag catgggatgc tgtg
//
LOCUS       DANJFZ010000128         6836 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.128, whole genome shotgun sequence.
ACCESSION   DANJFZ010000128 DANJFZ010000000
VERSION     DANJFZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.128"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            272..1501
                     /locus_tag="PWN45_002377"
     CDS             272..1501
                     /locus_tag="PWN45_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL0624119.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1696..2553
                     /locus_tag="PWN45_002378"
     CDS             1696..2553
                     /locus_tag="PWN45_002378"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL0624120.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2553..3497
                     /locus_tag="PWN45_002379"
     CDS             2553..3497
                     /locus_tag="PWN45_002379"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDL0624121.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3766..4734
                     /gene="pfkA"
                     /locus_tag="PWN45_002380"
     CDS             3766..4734
                     /gene="pfkA"
                     /locus_tag="PWN45_002380"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL0624122.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4756..6513
                     /gene="pyk"
                     /locus_tag="PWN45_002381"
     CDS             4756..6513
                     /gene="pyk"
                     /locus_tag="PWN45_002381"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL0624123.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2417 a          910 c         1381 g         2128 t
ORIGIN      
        1 ttttgtcgat atatcgtgcg gaaaaataat aactttttga tatattggtt tttgctatcc
       61 attataataa ttaaaggtat atctttagat agtatggttt aatgtatctt tataaatatt
      121 attttattat cgtttatcgc tatttgtgtt acataatcaa aaccataaat tcttactcat
      181 tcagatttac ccaatatttt tacttttata atgtaatgcg ttttatccaa gttatttttt
      241 aaaaataaat attgaattag gggctgattt catgtcatta agagatgaag cattggaaat
      301 gcacaaacgt aatcaaggta aattagaagt taaaccaaat gtaaaagtta ctaataaaga
      361 ggaattaagt ttagcatact cacctggcgt tgctgaaccg tgtaaagata tttatgaaga
      421 taaaagaaaa gtatatgatt acacaattaa aggaaataca gttgcagtta ttactgatgg
      481 aacagcggta ttaggtttag gtaacattgg acctgaagca agtattcctg taatggaagg
      541 taaagcagta ttattcaaaa gcttcgctgg tatcaatggg gtgcctattg cgttaaatac
      601 aactgatacc gaagaaatca ttaaaacagt taagttgtta gaacctaatt atggtggtat
      661 taatttagag gatatttcgg caccacgttg ttttgaaatt gaagaacgat tgaaaaaaga
      721 aactaatatt ccggtattcc atgacgatca acatggtaca gcaattgtaa cattggcagg
      781 tttggtaaat gcattgagag ttgttaacaa agatattgct aaaataaaag ttgtactaaa
      841 tggtgctggt gcagcaggaa tagccattgt taaattacta tacgcgtatg gtgtaagaaa
      901 tatggttatg tgtgactcaa gaggcgcaat ttttgaagga cgttcatatg gtatgaatcc
      961 tacgaaagat gttgtagcaa aatggacaaa taaagataag attgaagggt ctttagaaga
     1021 agtcgtaaaa gatgcagatg tatttatcgg ggtttctgta gctaatgcgc tgtcacaaga
     1081 tatggttaag agtatggcag ataatccaat tatatttgca atggctaatc caaatcctga
     1141 aataatacct gatgatgcca aagcggcagg tgcacgagtt gttggtacag gacgttcaga
     1201 ctatcctaac caaattaata atgtattagc tttccctggt atttttagag gtgcattaga
     1261 ggttgaagct acacatataa atgaagaaat gaaaaaggca gctgtagaag cgattgctga
     1321 tttaatcgat agttctgaat taaatgaaga ctactgtatc ccaggaccgt ttgataaacg
     1381 tgtagcgcca tcagttgctc gtaatgttgc taaagcggca atggaatctg gagtagctag
     1441 gattgaagtt gatccgcaag atgtgtatga taaaacaatg aaacttacag atttacaata
     1501 aaaataatta acgtttaatt aaattgctta aaagttagca ataaagagtc attaaaatga
     1561 tgaaaatgaa agtttatgat aaacattcaa cagtcaaacg aatataaatc aaataaattt
     1621 aaacccgttt ttaactggtc aagttcagtt taaggctcta aatggttaga acagaggtta
     1681 tttggaggtt ttcctatgtt taaagatttt tttaatcgaa caaagaaaaa gaaatatctt
     1741 acagtacaag actctaaaaa taatgatgtg cctgcaggta ttatgactaa gtgtccaaag
     1801 tgtaagaaaa ttatgtacac aaaagaatta gctgaaaatt taaatgtgtg ctttaattgt
     1861 gatcatcata ttgctttaac tgcgtataaa cgtatagaag caatttctga tgaaggatca
     1921 tttacagaat tcgataaggg aatgacctct gcgaatccat tagattttcc aagttattta
     1981 gaaaaaattg aaaaggacca acaaaagaca ggtcttaaag aagcagttgt gactggtaca
     2041 gcacaactag atggtatgaa atttggcgtt gctgtcatgg attcacgttt tagaatggga
     2101 agtatgggat cggttatcgg tgaaaagata tgtcgcatca ttgattactg cactgagaac
     2161 cgtttaccat ttattctttt ctctgcaagt ggtggtgcac gtatgcaaga aggtattatt
     2221 tccttgatgc aaatgggtaa aaccagtgta tctttaaaac gtcattctga cgctggacta
     2281 ttatatatat catatttaac acatccaact actggtggtg tatctgcaag ttttgcatca
     2341 gttggtgata taaatttaag tgagccaaaa gcgttgatag gttttgcagg tcgtcgagtt
     2401 attgaacaga caataaacga aaaattgcca gatgatttcc aaactgcaga atttttatta
     2461 gagcatggac aattggataa agttgtacat cgtaatgata tgcgtcaaac attgtctgaa
     2521 attctaaaaa tccatcaaga ggtgactaaa taatgttaga ttttgaaaaa ccactttttg
     2581 aaattcgaaa taaaattgaa tctttaaaag aatctcaaga taaaaatgat gtggatttac
     2641 aagaagaaat tgacatgctt gaagcgtcat tggaacgaga aactaaaaaa atatatacaa
     2701 atctaaaacc atgggatcgt gtgcaaattg cgcgtttgca agaaagacct acgaccctag
     2761 attatattcc atatatcttt gattcgttta tggaactaca tggtgatcgt aattttagag
     2821 atgatccagc aatgattggt ggtattggct ttttaaatgg tcgtgctgtt acagttattg
     2881 gacaacaacg tggaaaagat acaaaagata atatttatcg aaattttggt atggcgcatc
     2941 cagaaggtta tcgaaaagca ttacgtttaa tgaaacaagc tgaaaaattc aatcgtccta
     3001 tctttacatt tatagataca aaaggtgcat atcctggtaa agctgctgaa gaacgtggac
     3061 aaagtgaatc tatcgcaaca aatttgattg agatggcttc attaaaagta ccagttattg
     3121 cgattgtcat tggtgaaggt ggcagtggag gtgctctagg tattggtatt gccaataaag
     3181 tattgatgtt agagaatagt acttactctg ttatatctcc tgaaggtgca gcggcattat
     3241 tatggaaaga cagtaatttg gctaaaattg cagctgaaac aatgaaaatt actgcccatg
     3301 atattaagca attaggtatt atagatgatg tcatttctga accacttggc ggtgcacata
     3361 aagatattga acagcaagct ttagctatta aatcagcgtt tgttgcacag ttagattcac
     3421 ttgagtcatt atcacgtgat gaaattgcta atgatcgctt tgaaaaattc agaaatatcg
     3481 gttcttatat agaataatca acttgagcat ttttatgtta aatcgatact gggttttacc
     3541 ataaattgaa gtacattaaa acaataattt aatatttaga tactgaattt taactaagat
     3601 tagtagtcaa aattgtggct actaatcttt ttttaattaa gttaaaataa aattcaatat
     3661 ttaaaacgtt tacatcaatt caatacatta gttttgatgg aatgacatat caatttgtgg
     3721 taatttagag ttaaagataa atcagttata gaaaggtatg tcgtcatgaa gaaaattgca
     3781 gttttaacta gtggtggaga ttcacctgga atgaatgctg ccgtaagagc agttgttcgt
     3841 acagcaattt acaatgaaat tgaagtttat ggtgtgtatc atggttacca aggattgtta
     3901 aatgatgata ttcataaact tgaattagga tcagttgggg atacgattca gcgtggaggt
     3961 acattcttgt attcagcaag atgtccagag tttaaggagc aagaagtacg taaagttgca
     4021 atcgaaaact tacgtaaaag agggattgag ggccttgtag ttattggtgg tgacggtagt
     4081 tatcgcggtg cacaacgcat cagtgaggaa tgtaaagaaa ttcaaactat cggtattcct
     4141 ggtacgattg acaatgatat caatggtact gattttacaa ttggatttga cacagcatta
     4201 aatacgatta ttggcttagt cgacaaaatt agagatactg cgtcaagtca cgcacgaaca
     4261 tttatcattg aagcaatggg ccgtgattgt ggagatctag cattatgggc tggattatca
     4321 gttggtgctg agacaattgt agttccagaa gtgaaaacag atattaaaga aatagctgat
     4381 aaaattgaac aaggtattaa acgtggtaag aaacactcaa tcgttcttgt agcagaaggt
     4441 tgtatgactg cgcaagattg tcaaaaagaa ttatcacaat acatcaatgt tgataataga
     4501 gtgtctgtgt taggtcacgt tcaacgtggt ggtagcccaa caggtgcgga tagagtttta
     4561 gcatcacgtt taggtggata tgcggtagac ttattaatgc aaggtgaaac agctaagggt
     4621 gttggaatta agaacaataa aattgtagca acatcttttg atgaaatttt tgatggtaaa
     4681 gatcataaat ttgattatag tctatatgaa cttgctaaca agttatctat ataagatttc
     4741 aggaggaatt ataaaatgag aaaaactaaa attgtatgta caattggacc agcttcagaa
     4801 tcagaagaaa tgattgagaa attaatcaat gctggtatga acgttgcacg attaaacttt
     4861 tcacatggta gtcatgaaga gcataaaggt agaattgata caattcgtaa agtagctaaa
     4921 agattagaca aaattgtagc aattttatta gatacaaaag gtccagaaat tcgtacgcat
     4981 aatatgaaag acggtatcat tgaacttgaa cgtggcaacg aagttattgt tagcatgaat
     5041 gaagttgaag gaacacctga aaagttctca gtaacatatg aaaacttaat taacgatgtt
     5101 caagtaggtt catacatttt acttgatgat ggcttaattg aattacaagt taaagatatt
     5161 gaccatgcta aaaaagaagt taaatgtgat attttaaact ctggtgagct taaaaacaaa
     5221 aaaggtgtta acttacctgg cgtaagagta agtttacctg gtattacaga aaaagatgct
     5281 gaagatatcc gtttcggtat taaagaaaat gttgacttca ttgcagcaag tttcgtacgt
     5341 cgtcctagtg atgttttaga aattcgtgaa attttagaag aacaaaaagc taacatttca
     5401 gtattcccta aaattgaaaa ccaagaaggt attgataata ttgcggaaat tcttgaagtg
     5461 tctgatggtt taatggttgc acgtggtgac atgggtgttg aaattccacc tgaaaaagta
     5521 ccaatggttc aaaaagattt aatcagacaa tgtaacaaat taggtaaacc agttattaca
     5581 gctacacaaa tgttagattc tatgcaacgt aacccacgtg ctacacgtgc agaagctagt
     5641 gacgttgcca acgcaatcta tgatggtaca gatgcagtaa tgttatctgg tgaaactgct
     5701 gctggtttat atcctgaaga agctgttaaa acaatgagaa atattgctgt atcagctgaa
     5761 gcagcccaag attacaaaaa gttattgtca gatcgtacta aattagttga aacttcatta
     5821 gtgaatgcta tcggtatttc ggttgcacat acagctttaa acttaaatgt taaagcaatt
     5881 gtagctgcta ctgaaagtgg ttcaacggca cgtactatct ccaaatatcg tccacattca
     5941 gacattattg cggtgactcc aagtgaagaa actgcacgtc aatgttcaat tgtttgggga
     6001 gttcaacctg tagttaaaaa aggacgtaag agtacagatg cattgttaaa caatgcagtt
     6061 gcaacagctg ttgaaactgg tagagtatct aatggtgatt taatcattat tactgctggt
     6121 gtaccaactg gtgaaactgg aactactaat atgatgaaaa tccacctagt tggtgacgaa
     6181 attgctaatg gtcaaggtat tggacgtgga tcagttgttg gtactacgtt agttgctgaa
     6241 actgttaaag atttagaagg taaagattta tctgacaaag ttatcgttac taactcaatc
     6301 gatgaaacgt ttgtacctta tgtagaaaaa gctttaggct taattacaga agaaaatggt
     6361 attacatcac caagtgcaat tgttggttta gaaaaaggta ttccaacagt tgtaggtgta
     6421 gaaaaagctg ttaaaaacat aagcaataac atgttagtta cgattgatgc tgctcaaggt
     6481 aaaatctttg aaggatatgc aaacgtacta taatttataa aaaaacgtct ttccatttat
     6541 caacaatgga aaggcgtttt ttggttcatc tggtatttta tgacgtaatt aataggttat
     6601 ttgataatga tagtgtatga atggcaatct atataaatgt ttatatcttt tatacatgta
     6661 cattatcacc ttcaaacttt cactcatatt actttggaaa tttattataa aatagaagta
     6721 tggatgtatt tctgaaatga tacattatta aatagatgag aaagtaaaag ttttgagcca
     6781 agtacgcaat ttaatattat aagttgcata taaaacagga tgggacataa atccct
//
LOCUS       DANJFZ010000129         6772 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.129, whole genome shotgun sequence.
ACCESSION   DANJFZ010000129 DANJFZ010000000
VERSION     DANJFZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.129"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            319..720
                     /locus_tag="PWN45_002382"
     CDS             319..720
                     /locus_tag="PWN45_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0624124.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(840..1403)
                     /locus_tag="PWN45_002383"
     CDS             complement(840..1403)
                     /locus_tag="PWN45_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0624125.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1400..2353)
                     /locus_tag="PWN45_002384"
     CDS             complement(1400..2353)
                     /locus_tag="PWN45_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL0624126.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2463..3644
                     /locus_tag="PWN45_002385"
     CDS             2463..3644
                     /locus_tag="PWN45_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0624127.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3932..6349
                     /locus_tag="PWN45_002386"
     CDS             3932..6349
                     /locus_tag="PWN45_002386"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL0624128.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6371..6682
                     /locus_tag="PWN45_002387"
     CDS             6371..6682
                     /locus_tag="PWN45_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0624129.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2271 a          958 c         1296 g         2247 t
ORIGIN      
        1 gaagaaatat atgtagaatt gttgcaattt aatggtaata ttgatatatt ttctttgtat
       61 ataaattata aaattaatat gtaatagagt gatttgtttt atgtactatt atcttatttc
      121 taaatattaa ctctattgat tattggtttt tatacttatt taattttatt caactttgac
      181 aattgaatag aaagcaagtt tatttacact tgtagtttta tgcataagtt agcacataca
      241 agttttggga ttgttgggat gtttgttaat acttgtatag tagctaaata tgtgattatt
      301 aattgggaga tgtttagcat gaaaaaagta aataacgaca ctgtatttgg aattttgcaa
      361 ttagaaacac ttttgggtga cattaactca attttcagcg agattgaaag cgaatacaaa
      421 atgtctagag aagaaatttt aattttacta actttatggc aaaaaggttc tatgacgctt
      481 aaagaaatgg acagatttgt tgaagttaaa ccgtataagc gtacgagaac gtataataat
      541 ttagttgaat tagaatggat ttacaaagag cgtcctgttg acgatgaaag aacagttatt
      601 attcatttca atgaaaagtt acaacaagag aaagtagagt tgttgaattt catcagtgat
      661 gcgattgcaa gtagagcaac agcaatgcaa aatagtttaa acgcaattat tgctgtgtaa
      721 gtttaatagc ataaaaagag gttttcatta agttgaaaac ctctttttgt tgttggcatt
      781 aatttttcaa atgttgacta ctcaatccta aattataaat agtatagcgc agcaaatgct
      841 taagaaattt tttctatggc acaaatgaat ggagcatgat tacgttggtt taaaaattga
      901 tattgcaaaa cttgcgcatg cttttgatcc aaagtactca agtaatcaag caatgcatgc
      961 ttctcaattt gtccttcgct atgaccatga tatataacaa gtacaataat accttcaatt
     1021 gacattaatg atagcaatga attaatagct tggattgtcg tgtcaggctt tgtcacgata
     1081 gatttatcac ctttaggcaa ataacctagg ttaaagatgg ctgcatcaat atgaccttta
     1141 tgtgcatcat ttatatgatg ttcaatattt tcatgtccat cttttattaa agaaacatga
     1201 ttgaaatcct taactttatc acgtgtattt tccaaagcta aatcttgaat gtcgaaacca
     1261 taaacatgtc cttctggtac ttgttcggct aaaaataaag tgtcattgcc gttaccgcaa
     1321 gttgcgtcta caacaatact ttctggtgtt atatgttgtt taataagtgt ttttgaaaaa
     1381 gggagtatac gttctaattt cattgcttca ccttagactt gtaacgcaag ccttgataag
     1441 aatttctacg tgctaattca gcatcgatgc catttaatac ttcccattta ttaacactcc
     1501 acattggacc taccatgata tctattggac catcaccggt aattcggtga acgatcattt
     1561 cagggggaat cacttctaat tggtccacaa ctaggtttgt gtactcttct tgagtcataa
     1621 aagttaataa acctttatcg tattgtttta ccatcggtgt accttttaac aaatgaagta
     1681 aatgaatttt aataccttgt acatccattt gtgcaacttc tttggcagta gccatcatca
     1741 tgtcatagtc ttcgccaggt aagccattaa tgatgtgtgt acatacattg atattatgct
     1801 tacgtaattt tgccacacca tcataataag ttttcatatc atgggcacga ttgattaaat
     1861 cagatgttga ctgatggatt gtttgtagtc ctaattcaac ccataagtat gttcgttgat
     1921 tcaaatctgc taaatattcg acaacatcgt ctggtagaca gtcaggacgc gtaccaatag
     1981 ataatcccac aacacccggt tctttaagta caggttcgaa tttttctttt aatacttcaa
     2041 ccggtgcatg tgtatttgta aatgcctgaa aataagcaat atattttcct tcgtgccatt
     2101 tctcatgcat cttttcttta atttctttaa attgtactgc gattgaatct gcacgattac
     2161 ctgcaaagtc tccgctacct gcagcagaac aaaatgtaca tccaccatgt gctacagtgc
     2221 catcgcggtt aggacagtca aacccgccat ccaatgcaac tttaaatatt ttttgtccaa
     2281 atttattttt taaatggtaa ttccatgtgt gataacgttt gttttcaaaa gcgtattgga
     2341 aatgattgcc catatgtcat tttcctttct ataaaaaaag agttctaagt acagatttta
     2401 acatatttta atgttatagt gtttattata gtttgacaaa aaagagagag gaactatgaa
     2461 atatgaatat acctaaatca gtctggtggc tagtaattgg catggcgtta aatattactg
     2521 gttccagttt tttgtggcct ttaaatacaa tttatatgaa acaagaactt ggaaaaagtt
     2581 taactgttgc tggtttagtg ctaatgataa attcatttgg catggttatt ggaaacttat
     2641 taggtggttc actatttgat aaattaggtg gatacaagac gattttaatt ggaactttca
     2701 cttgtctttg tagtacaacg ctacttaatt tctttcacgg gtggccttgg tatgctgtat
     2761 ggcttgtaat gttagggttt ggtggcggaa tgattattcc tgcgatatac gctatggctg
     2821 gagcagtgtg gccaaatggc ggaagacaaa cgtttaatgc gatatactta gcgcaaaata
     2881 ttggtgtggc tgtcggtgct gcaatgggcg gctttgtcgc agaatttagc tttaactata
     2941 tctttttagc caatcttatt atgtatgttg tgtttgcgct tgtcgcggta acgcaattta
     3001 atattgaaat taatgcgaaa gttaaatatc caactcattt agatattact ggtaaaaaga
     3061 ataaagcaag atttatttca ttagtactaa tttgtgcaat gtttgcaatt tgttgggttg
     3121 catatattca atgggagtct acaatcgctt catttacaca atctattaat atttcaatgg
     3181 cacaatatag tgttttatgg acaattaacg gaataatgat tttagtagca caaccattaa
     3241 ttaaaccgat tctctatctg ttaaaaggaa acttaaagaa gcaaatgttt gtcggcatca
     3301 tcatttttat gttgtcgttc tttgtcacga gttttgccga aaactttaca atatttgttg
     3361 tcggtatgat tattttaact tttggagaaa tgtttgtatg gccagcagtt ccaactatag
     3421 ccaatcagtt agcgccagat ggtaagcaag gacagtacca aggttttgtg aattcagctg
     3481 ctacagtagg aaaagcattt ggtccatttc ttggtggtgt attagttgat gcgtttaata
     3541 tgcgcatgat gtttatcggt atgatgctac tacttgtatt tgcattaata ttattaatgg
     3601 ttttcaagga gaataatacg caacctaaaa aaatagatgc ataatgagta aatagaatta
     3661 acgttataga cttgaaataa atgtcgttat aacataatat taatttgtat aatttaattt
     3721 cgtttggagc ttttctacag aaagctagtg atgctgagag ctagtgttaa ggactaaatg
     3781 taaatcgtat taattttaaa ttgaatgaat gacatctctt actattaaaa tgagtgcaca
     3841 atttttgtga aatagggtgg taacgcggca aatgtcgtcc ctatgtaaat agaatagtta
     3901 gaggtgtctt ttttattgaa taggaggaaa tgtgttgaat tataaccaca atcaaattga
     3961 aaagaaatgg caagactatt gggacgaaaa taaaacattt aaaacaaatg ataacttagg
     4021 tcaaaagaaa ttttatgctt tagacatgtt tccatatcca tcaggtgctg gtttacatgt
     4081 tggacatcct gagggctata cagcaacaga tatcatttca agatataaaa gaatgcaagg
     4141 atataatgta ttacatccga tggggtggga tgcattcgga ttaccagcag agcaatatgc
     4201 tttagacact ggcaacgacc cacgtgaatt tacaaagaaa aatatccaaa cttttaaacg
     4261 acaaattaaa gaattagggt tcagttatga ttgggatcgt gaagttaata caacagatcc
     4321 agaatactat aaatggacac agtggatttt catacagtta tataacaaag gtttagcata
     4381 cgttgatgaa gttgcagtta actggtgtcc agcattaggc actgttttat ctaacgaaga
     4441 agtgattgat ggtgtctctg aacgtggtgg acatccagtt tatcgtaagc cgatgaaaca
     4501 atgggtactt aaaatcacag aatatgcaga tcaattatta gcagatttag atgatttaga
     4561 ttggcctgag tctttaaaag atatgcagcg caattggatt ggacgttctg aaggggccaa
     4621 agtttcattt gatgtagata atacggaagg aaaagtagaa gtatttacga ctagaccaga
     4681 tacaatctat ggtgcatcat tcttagtctt aagtcctgaa catgcattag ttaattcaat
     4741 tacaacagat gaatataaag aaaaagtaaa agcttatcaa acagaagctt ctaaaaagtc
     4801 agatttagaa cgtacagatt tagcaaaaga taaatcaggt gtatttactg gtgcatatgc
     4861 aactaatcct ttatctggtg aaaaagtaca aatttggatt gctgattatg tattatcaac
     4921 atatggtact ggagcaatta tggcagtacc agcgcatgat gacagagatt atgaatttgc
     4981 taaaaagttt gatttgccaa tcattgaagt catcgaaggt ggaaatgttg aagaagcagc
     5041 atacactggt gaaggtaaac atattaattc tggtgaactt gatggtttag aaaatgaagc
     5101 ggcaattact aaagctattc aattattaga gcaaaaaggt gctggcgaaa agaaagttaa
     5161 ttacaaatta agagattggt tattcagtcg tcagcgttat tggggcgaac caattcctgt
     5221 cattcattgg gaagatggaa caatgacaac tgttcctgaa gaagagctac cattgttgtt
     5281 acctgaaaca gatgaaatca agccatcagg gactggtgag tctccactag ctaatattga
     5341 ttcatttgta aatgttgtag atgaaaaaac aggtatgaaa ggacgtcgtg aaacaaatac
     5401 aatgccacaa tgggcaggta gttgttggta ttatttacgt tacatcgatc ctaaaaatga
     5461 aaatatgtta gcagatcctg aaaaattaaa acattggtta cctgttgatt tatatatcgg
     5521 tggagtagaa catgcggttc ttcacttatt atatgcaaga ttttggcata aagtccttta
     5581 tgatttggct atcgtaccta ctaaagaacc tttccaaaaa ttatttaacc aaggtatgat
     5641 tttaggagaa ggtaatgaga agatgagtaa atctaaagga aatgtaatca atcctgatga
     5701 tatagtacag tctcatggtg cagatacttt gcgtctttac gaaatgttta tgggaccttt
     5761 agatgctgca attgcatgga gtgaaaaagg attagatggg tctcgtcgat tcttagatcg
     5821 cgtatggcgt ttaatggtaa atgaagatgg gacattgagt tcaaaaattg taactacaaa
     5881 taataaatct ttagataaag tttataacca aactgttaaa aaggtaacag aagactttga
     5941 aacattagga tttaatactg ctattagtca attaatggta tttattaatg agtgttataa
     6001 agttgatgaa gtttataaac cttacattga aggcttcgtt aaaatgttag cacctattgc
     6061 accacatatc ggtgaagaat tatggtcaaa attaggacat gaagagtcta ttacgtacca
     6121 accttggcca acttatgacg aagcactact tgtagatgat gaagtagaaa tcgttgttca
     6181 agtgaatggt aaattgagag ctaaaattaa aattgctaaa gatacatcaa aagaagaaat
     6241 gcaagaaatt gccttatcta atgacaatgt taaagcgagt attgaaggta aagacatcat
     6301 gaaagtcatc gctgttcctc aaaaattagt caatattgta gctaaataat gttttaagga
     6361 ggactttgaa atgaagtcaa ttactacaga tgaattaaaa aataaacttt tagaatctaa
     6421 accagttcaa attgttgatg ttcgtactga tgaagaaaca gcaatgggat atattcctaa
     6481 tgcaaagtta attccaatgg ataccattcc ggataattta aattcattta ataaaaatga
     6541 aatatattat attgtatgtg ctggtggagt tcgaagcgct aaagttgtag aatatttaga
     6601 ggcaaatggc attgatgccg taaatgtcga aggcggcatg cacgcatggg gcgatgaagg
     6661 tttggaaata aaaagtattt aaagtagtga cataatttaa aataatatta catttgtaat
     6721 gacaccaagt aacgtttcgg ttgcttggtg ttttttggta tgaattactt tc
//
LOCUS       DANJFZ010000130         6616 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.130, whole genome shotgun sequence.
ACCESSION   DANJFZ010000130 DANJFZ010000000
VERSION     DANJFZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.130"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(142..350)
                     /locus_tag="PWN45_002388"
                     /pseudo
     CDS             complement(142..350)
                     /locus_tag="PWN45_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(811..1598)
                     /locus_tag="PWN45_002389"
                     /pseudo
     CDS             complement(811..1598)
                     /locus_tag="PWN45_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            complement(1611..2150)
                     /locus_tag="PWN45_002390"
     CDS             complement(1611..2150)
                     /locus_tag="PWN45_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624130.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            2949..3884
                     /gene="lukE"
                     /locus_tag="PWN45_002391"
     CDS             2949..3884
                     /gene="lukE"
                     /locus_tag="PWN45_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDL0624131.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            3886..4869
                     /gene="lukD"
                     /locus_tag="PWN45_002392"
     CDS             3886..4869
                     /gene="lukD"
                     /locus_tag="PWN45_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDL0624132.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            5111..5254
                     /locus_tag="PWN45_002393"
     CDS             5111..5254
                     /locus_tag="PWN45_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDL0624133.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
     gene            5991..6134
                     /locus_tag="PWN45_002394"
     CDS             5991..6134
                     /locus_tag="PWN45_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDL0624134.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            6199..>6616
                     /locus_tag="PWN45_002395"
     CDS             6199..>6616
                     /locus_tag="PWN45_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDL0624135.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEY"
BASE COUNT         2419 a          854 c         1011 g         2332 t
ORIGIN      
        1 agtaaagcgt agcataacca ggagttagtc gatttttaat aaattcgact aaattattaa
       61 taaaggctaa agtaattagt ttactttagc ctttattgca tttgtaattt atagaactgc
      121 gaacaggtga ctactgttaa tttatgtgat taaagtttca atgatatttg atttattaga
      181 ccatttcaaa atcgcaaggt tctcatgttc tgctacagtt tgcggtcttt ggaagatata
      241 tcatttacta ttattaacat gatatattct aatttgtttg tggatgggct gtttggtttg
      301 atatcgcggt aaatataagc atcattagtt atattgttga aatctaagtt gaatttatta
      361 tcatacagca atgtgccaat tagggacatt tttaattttg aactctacat tttgttgaga
      421 gtatctggtt atatttgcaa aatggtatga acatttatca tcacttatta agttgtcttg
      481 atgattgaat gacccagtat ttattcagtt cgagaagatt gagagtgtta aatcgtattc
      541 gagtataatt tcgattctag tcataccatt ttcataaagt ttagccattt gtaacttaaa
      601 ctatgaacta aataatcttc tgactcttgt caaaataaaa tcgcctaccc aaatagttta
      661 acaatatcta ttctcataga attagttcaa ctaagtgtag acgattctgc attatcaata
      721 aggctattca atttgctatc ttaagctgtt tcgaaatttt caaattcgtt tcatttaata
      781 taaacattga tattatcacg tctatatata ttaaatcaat aaacctatct tttcatctaa
      841 ttcgctataa tctattgtac taatatagtt ttcatgaaga agatttttat tatgttcatt
      901 tatttctttt tttgagtcat ttaaaataaa aataaactca ttttgcctgt gtctttttat
      961 ttttttgctt gcatagcatc cgttattttc gacttaataa tcatagaatt attagggtta
     1021 gaaatagcat tgataaattt ttctttttga tttttcttag cgctaataaa aaagtcgaaa
     1081 ttatgaacaa caccactgct tcctataata ggtaaatctc ttccgtaata aatgttatgt
     1141 tcatccaatt tgtttgcaac atcttctgta aatatgttct gtgacttagg attagaaagt
     1201 aagtacatat cattaacaaa tataaggcac tgtaataaat tatgtttcga tttattaaaa
     1261 tttttaaagt tagtttgaac aaaaatttcg tgagttttat cgttatattt aataccgtaa
     1321 gctgaaaggt gctcttcaaa aatcttttta cgtttttttg atttatttaa agaaatacca
     1381 ttattttcta aatcaaatat agtatagccg tcatctgtca gtgtaatcat gtctctggac
     1441 tgatcgtata aagcgtaaat tattaaatta tctaaagaat tatctttaaa aggagtatct
     1501 attcttacta cattgtttga taaattagaa aactctaagg tctgattata ccaatcaaga
     1561 tattcttttt ttaatttttt agcatcaaat gtagtcatat tatccctcct ttattcaagg
     1621 tcttttatat tattatactc taaaaacgac atatacacat tatacaagtt atagatgtcg
     1681 gggaaatctt tcaagttaat ttcataagca aacctatcct ttttatcata cttatcatta
     1741 tatatgtgaa tatgactttt cggagcgatt gttccatctg gattatcgtg agtaccaccg
     1801 ttaatatcta ttctaactag ggtgtgatag gtatctttga aaatgattga aattgaaaac
     1861 ctatcttttt ctatgttccc tctttttatt gttaataagt actttattcc taacttaaaa
     1921 cttattattt cttgcctttc atttattcga ctagcaacta ttgtttttaa ttcatttaca
     1981 tcgatatgag tttctggata ttttaattca tttattaata attttacgtg ctcgtcgtca
     2041 agactattta aatcaagttt agccatgtct gttcctgtat aggcattaat ttttaatact
     2101 ttcttatttg aaaaaagcaa aaaagatagg tgagatgcat acgcaaccaa aagtgtataa
     2161 tatcaattta ctacctcgca atataatttt atacaatgta taataagatt taaagatgta
     2221 gctcatcaac attttcatag gggattttta aaattaagta ttgaaaaata aaatatttat
     2281 attataatat aaataagtca acgaccatgc gtggacagta aaacgaagta catgcgaatg
     2341 tagtagccgt ttactatgag actaggcatt ataattgaat atcatagtat aaattgccat
     2401 gagagtagaa gcttcagttt atgaagactt cgaaatcatg gttttttatt tttaaagtga
     2461 aattttagtt catagtaaat atatatatgt ttttaattag actaaatcat agcattaaat
     2521 ttttttagat gaagtaagcg atattacgaa atgatatata gaacatttca gttataacag
     2581 tatacgaagt aatcatcttc gtttaacgga caatagttaa taatcagtta attttaaatt
     2641 caattataga ttatttgtga taatacaaac attatttttg aagatttgat taaagtcgtt
     2701 tttattttta cagagaaaag attgagtatc ggtaatttta tcatattgtt cgaatataaa
     2761 gggaactatt taaaggtaat gaaggttgcg ccaaaatgac cggttttcag cagcataaat
     2821 gaataaattg tgtcattttt aatattttat agcattttat tttttaatga acagttaatt
     2881 ttataaataa gtgaaataat ctagaataaa tttgtattaa aaatttatat agaaagaaag
     2941 tgaaacttat gtttaagaaa aaaatgttag ctgcaacttt gtcagtagga ctgattgcac
     3001 ctttagcatc tccgattcaa gaatctagag caaatactaa tattgaaaat attggtgatg
     3061 gtgctgaagt aatcaaacgt acggaggatg taagtagtaa gaaatggggc gttactcaaa
     3121 atgtccaatt cgactttgta aaagataaaa aatataacaa agacgcttta attgttaaaa
     3181 tgcaaggttt tattaattcc agaacttcat tttcagatgt gaagggtagt ggatatgaat
     3241 taactaaacg aatgatttgg ccattccaat ataatatagg actgacgact aaagatccaa
     3301 atgttagctt aatcaattac cttcctaaaa acaaaataga aactactgat gttggtcaaa
     3361 cattaggata taacattgga ggtaatttcc agtcagcacc atctataggt ggcaatggct
     3421 catttaatta ttctaaaaca attagttata cccaaaagag ttatgtcagt gaagtagaca
     3481 agcaaaactc aaaatctgtt aaatggggtg ttaaagcaaa cgaatttgtt acgcctgatg
     3541 gaaaaaaatc tgcgcatgat agatatttat tcgtacaaag tccaaatggt ccaacaggtt
     3601 cagcaagaga atattttgct cctgataatc aattgccacc tttagttcaa agtggcttta
     3661 atccatcgtt tatcactaca ctatcacatg aaaaaggttc aagtgatacg agtgaatttg
     3721 aaatttcata tggtagaaac ttagatatta catatgcgac tttattccct agaactggta
     3781 tttacgcaga aagaaagcat aatgcatttg taaatagaaa ctttgtagtt agatatgaag
     3841 ttaattggaa aacacacgaa attaaagtga aaggacataa ttaatatgaa aatgaaaaaa
     3901 ttagtcaaat catcagttgc ttcatcaatt gcactgcttt tgctatcgaa tacagttgat
     3961 gcagctcaac atatcacacc tgtaagcgag aaaaaagtag atgacaaaat cactttatac
     4021 aaaacaacag caacatctga taatgataaa ttgaatattt ctcaaatttt aacgtttaat
     4081 ttcattaagg ataaaagtta tgacaaagat acgttagtac ttaaggcagc cggaaacatt
     4141 aattcaggtt ataaaaagcc taatccaaaa gattacaatt actcacagtt ttattggggc
     4201 ggtaagtata atgtttcggt tagttcagaa tcaaatgatg ctgtaaatgt tgttgactat
     4261 gcacctaaaa atcaaaatga agaattccaa gttcaacaaa cattaggtta ttcttatggc
     4321 ggagatatta atatatctaa tggcttatca ggtggattaa atggatcaaa atcattttca
     4381 gaaacgataa attataaaca agaaagttac agaactacga ttgatagaaa aacaaatcat
     4441 aaatcaattg gctggggtgt tgaggcgcac aaaattatga ataatggttg gggaccatat
     4501 ggtagagata gttatgaccc aacatatggt aatgaactgt ttttaggcgg tagacaaagt
     4561 agttcaaatg ctggtcaaaa tttcttgcca acacatcaaa tgcctttatt ggcgcgtggt
     4621 aactttaacc cagaatttat aagcgtactt tctcataaac aaaatgatac aaaaaaatct
     4681 aaaatcaaag taacttacca aagagaaatg gatagatata ctaatcaatg gaatcgacta
     4741 cactgggttg gtaataacta caaaaatcaa aatacagtaa cgtttacatc tacttatgaa
     4801 gttgactggc aaaaccatac tgttaaatta atcggtacgg attctaaaga aactaatcct
     4861 ggagtataac tgttagaaat agaggaagtt aaacccgaaa tttaagagtt aaacaaaaaa
     4921 tattcacaac atattaaaaa aagaagttac attatttgtt taaactaaca atagtaccat
     4981 aaaattaatg acgatttaca aattaataac aaataattat tattaaattc attgatttat
     5041 tgtgtggtaa tgattaatat gacaattgaa gtgggtctta acttcaactt ttaaataggg
     5101 ggttttatct ttggaaaaag ttcttgattt agacgtgcaa gttaaaggaa ataacaacac
     5161 taatgattca gcgggtgacg aaagaataac tagccatctt ttttgtagct ttggttgtga
     5221 aaagacgggt agttttaaca gcttctgttg ttaaggatac agcaaataga gcctgaaagg
     5281 gctctatttg tctttttaaa tatattaagt gctaaaaaac ggaaacagtt tattaatcgc
     5341 caggtatttt tataattagc cttctatatt atcagttgag tgtttcaata aattctgact
     5401 ttggtgatgg aaagttatac ttttagttgt aatatagttt tcatgacttc ctgttaaata
     5461 agttaggatt atgtaattag cctctatctt tacatataag taatgacaag ctttggttat
     5521 tatattgacc ctcttagaac atgtcattta agaaaataat caagtcattg aaattatatg
     5581 tttatatcct catctttaaa ataacttcaa ttattatttg ctaaattttg tataaataga
     5641 acaaaactgc ataaatatct attaattaat ctcaagcagc catctccaaa ttcgtaatag
     5701 tattgataga taaaaatatc taagtatttg tataaatgaa ttgattaatc tattaaaaaa
     5761 ttcatcaata aatttaaaat tatgaactta gatgcaaaat tattagtgaa aataaatagt
     5821 aataaaaaaa cacaaaaatc taattatcaa aatagtatta ctaaattaat aagtgtttac
     5881 aaaaaattca caaaatataa ttatataatt tattgatttg ttgtgtgaaa agggataata
     5941 tgacaattga agtaggcctt aacttcaact aataatagga ggttttatct atggaaaaag
     6001 ttcttgattt agacgtgcaa gttaaagcaa acaataactc aaatgattca gcaggtgacg
     6061 aacgtattac aagtcatagt ttatgtactc ctggttgtgc taagactggt agttttaata
     6121 gcttctgctg ttaagattac agcaaataga gcctgaaagg gctctatttg tctattcaaa
     6181 tatgtgaggt gcttacaaat gaacactatt tacgaaccgt caagtatttg tatgattaga
     6241 acacctttat tatcagttga atttttcaat ttatttttaa atactgaaca aattaaatat
     6301 agcgacttac aattaaatgc tcagatgaaa gaatcaatcc taacaacaac atttaactta
     6361 tattgtactt tacaagaaat aaattttgat ggcgataaca agaaggttag agatgctaaa
     6421 gaaagtttat taaaatattt aattagaatg tcaacacgtc caactccatt tggattatta
     6481 agtggtatta atttggggca ttttgtaaat gaaccaacac gtcttaaagt tggaaatagt
     6541 attcaaaaat atgtgaaagt cgatggcgaa tggttgtata aactggttag ttatattgag
     6601 agtattgatg aatatt
//
LOCUS       DANJFZ010000131         6436 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.131, whole genome shotgun sequence.
ACCESSION   DANJFZ010000131 DANJFZ010000000
VERSION     DANJFZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.131"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..302
                     /locus_tag="PWN45_002396"
     CDS             <1..302
                     /locus_tag="PWN45_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDL0624136.1"
                     /translation="LTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSDLM
                     IEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            292..1983
                     /locus_tag="PWN45_002397"
     CDS             292..1983
                     /locus_tag="PWN45_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0624137.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            1988..3226
                     /locus_tag="PWN45_002398"
     CDS             1988..3226
                     /locus_tag="PWN45_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0624138.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3210..3983
                     /locus_tag="PWN45_002399"
     CDS             3210..3983
                     /locus_tag="PWN45_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0624139.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            3995..5158
                     /locus_tag="PWN45_002400"
     CDS             3995..5158
                     /locus_tag="PWN45_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0624140.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5227..5505
                     /locus_tag="PWN45_002401"
     CDS             5227..5505
                     /locus_tag="PWN45_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0624141.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5517..5849
                     /locus_tag="PWN45_002402"
     CDS             5517..5849
                     /locus_tag="PWN45_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624142.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5846..6247
                     /locus_tag="PWN45_002403"
     CDS             5846..6247
                     /locus_tag="PWN45_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624143.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6248..>6436
                     /locus_tag="PWN45_002404"
     CDS             6248..>6436
                     /locus_tag="PWN45_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624144.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEY"
BASE COUNT         2519 a          867 c         1220 g         1830 t
ORIGIN      
        1 aattaacaaa aaaacagctg aaagaatata tagaggatta taaaaaatct gatgacatat
       61 taattaattt gtatatagaa acgtatgaat tttattgtcg gttaagagat gaacttaaaa
      121 atagtgattt gatgatagag catacaaaca aggctggtgc gagcaatatt gttaagaatc
      181 cattaagcat agaactgaca aaaacagttc aaacactaaa taacttactc aagtctatgg
      241 gtttaactgc agcacaaaga aaaaagatag ttcaagaaga aggtggattc ggtgactatt
      301 aaagttttaa atgaaccttc accaaaacta ttaacaacat ggtatgcaga gcaagtcact
      361 caagggaaaa taaaaacaag caaatatgtt aaaaaagaat gtgagagaca ccttagatat
      421 ctagaaaatg gaggtaaatg ggtatttgat gaagaattag cgcaccgtcc tattcgattc
      481 atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc aacttgtatt acaaccatgg
      541 caacatttta ttattggcag tttgtttggt tgggttcata aagaaacaaa actgcgcagg
      601 tttaaagaag ctttgatatt tatggggcga aaaaatggta aaacaactac tatatctggt
      661 gttgctaact atgctgtttc tcaagatgga gaaaacggcg ctgaaatcca tcttttagca
      721 aacgtaatga aacaagctag aatattattc gatgaatcta aggcgatgat aaaagctagc
      781 ccaaagcttg ataaaaattt cagaacatta agagatgaaa tccattatga cgcaacgata
      841 tcaaaaatta tgccccaagc atcagatagc gataagttag atggattgaa tacacacatg
      901 gggatttttg atgaaattca tgaatttaaa gactataaat tgatttcagt tataaaaaac
      961 tcaagagctg caaggttaca acctcttctc atctacatta cgacagcagg gtatcaatta
     1021 gatggtccac ttgttgatat ggtagaagcg ggaagagaca ccttagatca aatcatagaa
     1081 gacgaaagaa ctttttatta tttagcatct ttggatgatg acgatgatat taatgattcg
     1141 tcgaactgga taaaagcaaa tcccaactta ggtgtctcta taaatttaga tgagatgaaa
     1201 gaagagtggg aaaaagctaa gagaacacca gctgaacgtg gagattttat aaccaaaagg
     1261 tttaatatct ttgctaataa tgacgagatg agttttattg attacccaac actccaaaaa
     1321 aataatgaaa ttgtttcttt agaagagctg gaaggcagac catgcacgat tggttatgat
     1381 ttatcagaaa cagaggactt tacagccgcg tgtgctactt ttgcgttaga taatggtaaa
     1441 gttgcagttt tatcgcattc atggattcct aagcacaaag ttgaatattc taacgaaaaa
     1501 ataccctata gagaatggga agaagatggc ttattaacag tgcaagataa gccttatatt
     1561 gactaccaag atgttttaaa ttggataatt aagatgaatg agcattatgt agtagaaaaa
     1621 attacttatg atagagcgaa cgcattcaaa ctaaatcaag agttaaaaaa ttacgggttt
     1681 gaaacggaag aaacaagaca aggagctttg accttgagcc ctgcattgaa ggatttaaaa
     1741 gaaatgtttt tagatgggaa aataatattt aataataatc ctttaatgaa atggtatatc
     1801 aataatgttc agttgaaact agacagaaac ggaaactggt tgccgtctaa gcaaagcaga
     1861 tatcgtaaaa tagatggctt tgcagcattt ttaaacacat atacagatat tatgaataaa
     1921 gttgtttctg acaagggtga aggaaacata gaatttatta gtattaaaga tataatgcgt
     1981 taaggaggtg aatgttatcg caaaagagaa tattgtcaca cgcataaaga aaaaattgat
     2041 agacaattgg attgatcagt cagcttctaa gctttatgac tttagcccat ggaaaaataa
     2101 atctttttgg ggtgtaatca ataatacgct tgaaactaat gaaacgatat tttcagctat
     2161 tacaaagtta tctaattcga tggctagttt gcccttgaaa atgtatgaag attataaagt
     2221 agttaataca gaagtatctg atttacttac agtgtcaccg aataattctc tgagcagttt
     2281 tgattttatt aatcaaattg aaacaatcag aaatgaaaaa ggtaatgcat atgtgctaat
     2341 tgaacgagac atctatcatc aaccatcaaa gcttttctta ttaaatccag atgttgttga
     2401 aatgttaatt gaaaaccaat cacgtgaact ttattattcc attcatgctg caactggaaa
     2461 taaattgatt gttcataata tggacatgtt gcattttaaa cacatcgtgg catctaatat
     2521 ggtgcaaggc attagtccga ttgatgtgtt gaagaataca actgattttg ataatgcagt
     2581 aagaaccttt aatcttacag aaatgcaaaa acctgattct ttcatgctta aatatggttc
     2641 caatgtaggt aaagaaaaaa ggcagcaagt gttagaagat ttcaaacagt actatgaaga
     2701 aaacggtgga atattattcc aagagcctgg tgttgaaatc gaaccgttac ctaaaaaata
     2761 tgtctctgaa gatatagtgg caagcgagaa tttaacaaga gaaagagtag ctaacgtttt
     2821 tcaattgccc tcagtattct taaatgcaag atcaaataca aatttcgcga aaaatgaaga
     2881 gttaaacaga ttttacttgc agcatacctt attgccaatc gtcaaacagt atgaagaaga
     2941 atttaatcgg aaactactta ctaaaacaga cagagaaaaa aataggtatt ttaaatttaa
     3001 cgttaaatct tatttaaggg ctgatagtgc aacacaagca gaagtgtact ttaaagcagt
     3061 tcgtagtggt tactacacta taaatgacat tagagagtgg gaagatttac cgccagttga
     3121 aggtggagat aagccgctaa taagcggtga tttataccca attgacacgc cacttgaatt
     3181 aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa agctaagtat tttcaaatga
     3241 aaagaaaatc aaaaagtaaa ggtgaaatat ttatttatgg tgatattgta agtgataaat
     3301 ggtttgaaag tgatgtaact gctacagatt tcaaaaataa actagatgaa ctaggagaca
     3361 tcagtgaaat agatgttcat ataaattcat ctggaggcag tgtatttgaa gggcatgcaa
     3421 tatacaatat gctaaaaatg catcctgcaa aaattaatat ctatgtcgat gccttagcgg
     3481 catcaattgc tagtgttatc gctatgagtg gtgacactat ttttatgcac aaaaatagtt
     3541 ttttaatgat tcataattca tgggttatga ctgtaggtaa tgcagaagaa ttaagaaaga
     3601 cagcggattt acttgaaaaa acagatgctg ttagtaattc agcttattta gataaagcaa
     3661 aagatttaga tcaagaacac ttaaaacaga tgttagatgc agaaacttgg cttactgcag
     3721 aagaagcctt gtctttcggc ttgatagatg aaattttagg agctaatgaa atagctgcta
     3781 gtatctctaa agagcaatat aagcgtttcg agaacgtccc agaagattta aagaaagatg
     3841 tagacaaaat cactaaaatt gatgatgtag atacatctga attggttgaa acacctaaag
     3901 aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat taaacgcgaa tgcgaaattt
     3961 taaaaatgac aatgaattat taggaggaaa tgaaatgccg acattatatg aattaaaaca
     4021 atccttaggt atgattggac aacaattaaa aaataaaaat gatgaattga gtcagaaagc
     4081 aacagatcca aatattgata tggaagacat caaacaacta gaaacagaaa aagcaggttt
     4141 acaacaaaga tttaacattg ttgaaagaca agtgcaagac attgaagaga aagaaaaagc
     4201 gaaagttaaa gataaaggag aagcttatca atctttaagt gataatgaga agatggttaa
     4261 agctaaggca gagttttatc gtcacgcgat tttaccaaat gaatttgaaa aaccttcaat
     4321 ggaggcacaa cgtttattac acgctttacc aacaggaaat gattcaggtg gagataagct
     4381 cttaccaaaa acactttcta aagaaattgt ttcagaacca tttgctaaaa accaattacg
     4441 tgaaaaagct cgtctaacta acattaaagg tttagagatt ccaagagttt catacacttt
     4501 agacgatgat gatttcatta cagacgtaga aacagcaaaa gaattaaaag caaaaggtga
     4561 tacagtcaag ttcactacta ataaattcaa agtatttgct gcaatttcag atactgtaat
     4621 tcatggatca gatgtagatt tagtaaactg ggttgaaaac gcactacaat caggattagc
     4681 agctaaagag cgtaaagatg ccttagcagt aagtcctaaa tctggattag aacacatgtc
     4741 attttataat ggatctgtta aagaagttga gggagcagac atgtatgatg ctattattaa
     4801 cgctttagca gatttacatg aagattatcg tgataacgca acaatttata tgcgatatgc
     4861 agattatgtc aaaattatta gtgttctttc aaatggaaca acaaatttct ttgacacacc
     4921 agcagaaaaa gtatttggca aaccagtagt atttacagat gcagcagtta aacctattgt
     4981 gggagatttc aattattttg gaattaacta tgatggaaca acttatgaca ctgataaaga
     5041 tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg tatgatcagc aacgtacatt
     5101 agacagtgca ttcagaattg caaaagcaaa agaaaataca ggtccattac ccagctaagc
     5161 cccaaaaggt taatgtaaca gctaaggcta aatcagctgt aatatcagcc gaataggggt
     5221 gatgaaatga gtttagaaga aattaaattg tggttgagaa ttgactataa tttcgaaaat
     5281 gatttaattg aaggtctcat tcaatcggct aagtctgaat tactattaag tggggttcca
     5341 gattatgaca aagatgactt ggaatacccg cttttttgta cagcgattaa atatatcatt
     5401 gcaagagatt atgaaagtcg tggatactca aatgaccaat ctagaagcaa ggtgtttaat
     5461 gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt ggtaggtgat ttttaaatgg
     5521 aatttaatga atttaaagat cgcgcgtatt tttttcaata tataaacaaa ggaccatatc
     5581 cagatgaaga ggaaaaaatg aaattgtata gttgcttttg taaaatttat aatccttcta
     5641 tgaaagatag agaaatttta aaagcgactg aatcaaaatc aggattaacc ataattgtca
     5701 ggtcttctaa aactgaatat ctaccacaaa caaatcactt agttaaaatt gacagtgcat
     5761 tatattccga taaattattc aacattgtag aaataagaat tgatacacca gatattggct
     5821 ataatacagt ggttttatca gaaaaatgag tgtagaaatt aaagggatac ctgaagtgtt
     5881 gaataaatta gaatcggtat acggtaaaca agcaatgcag gctaagagtg ataaagcttt
     5941 aaatgaagca tctgaatttt ttataaaggc tttaaagaaa gagttcgaga gctttaaaga
     6001 tacgggtgcc agtatagaag aaatgactaa atctaagcct tatacaaaag ttggcagtca
     6061 agaaagggct gttttaattg aatgggtagg ccctatgaat cgcaaaaaca ttattcactt
     6121 gaatgaacat ggttatacaa gagatggaaa aaaatataca ccaagaggtt ttggagttat
     6181 tgcaaaaaca ttagctgcta gcgaacgtaa gtatagagaa attataaaaa aggagttggc
     6241 cagataaatg aatatattaa acaccataaa aggaatttta ttatctgatg cagagctcaa
     6301 aacacatata aattctagaa tatactatta caaagtcact gaaaacgctg aaacttccaa
     6361 accctttgtt gttattacac ctgtttatga tttgccttca gactttatgt ctgataaata
     6421 tctcagtgaa gaatac
//
LOCUS       DANJFZ010000132         6430 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.132, whole genome shotgun sequence.
ACCESSION   DANJFZ010000132 DANJFZ010000000
VERSION     DANJFZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.132"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(503..1219)
                     /locus_tag="PWN45_002405"
     CDS             complement(503..1219)
                     /locus_tag="PWN45_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDL0624145.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(1327..2052)
                     /locus_tag="PWN45_002406"
     CDS             complement(1327..2052)
                     /locus_tag="PWN45_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDL0624146.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            complement(2147..2872)
                     /locus_tag="PWN45_002407"
     CDS             complement(2147..2872)
                     /locus_tag="PWN45_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDL0624147.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            3310..4311
                     /gene="argF"
                     /locus_tag="PWN45_002408"
     CDS             3310..4311
                     /gene="argF"
                     /locus_tag="PWN45_002408"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0624148.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            4334..5266
                     /gene="arcC"
                     /locus_tag="PWN45_002409"
     CDS             4334..5266
                     /gene="arcC"
                     /locus_tag="PWN45_002409"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0624149.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            5438..>6430
                     /locus_tag="PWN45_002410"
     CDS             5438..>6430
                     /locus_tag="PWN45_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL0624150.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWL"
BASE COUNT         2178 a          916 c         1118 g         2218 t
ORIGIN      
        1 tagatttaga acgattatta aactttatag ttactatgtc tcattaaaag aacactaatc
       61 atgggacaat aaagtattgc tataataatt taatcattaa atgaaggaat agagcctaag
      121 acataataaa tcaatgtctt aggctctaca atattatatt ggtagtagtt gaccgaatga
      181 aaatgcccat gtaacaagca tttttcaatt ataggcgggg ccccaacata gagaatttca
      241 aaaagaaatt ctacagacaa tgcaagttgg ggtgggcccc aacacagaag ctgacgaaaa
      301 gtcagcttac aataatgtgc aagttgggga gtgacaacga aataaatttt ataaaatact
      361 atttctgtca cattcctact cccaattcca tttaaatata tagaaaattt tacctattat
      421 atacatcata cactttaaaa attatcattt aaataatctg atttggaatt aaagtgcatg
      481 aagtataagt caccttcata tactaatcaa agaggacgtc aacagttatt ttattaggat
      541 ttttaacata aacatttgct agatctgaat gtaatctttt gcttaaatca atagtgtagt
      601 tattaccgcc accggtgatc ttaagcttac ctttattacg attttcgtta tataatattt
      661 tattttttat taacgcttct cgtgcacgga aatcgatttc tttcaatgtt aatactggtt
      721 tattgccttg gtatatttta tgcgcaccaa taatcgtttg tagtttatct ttgtattgca
      781 caaaaagatt ataagtttta tcagaaggtt ttgcggctgg tgtaacgcca cctgtaaatg
      841 tctctctata agaccaccat aactgatcag tatctttgtc ttttagtcca aacacatcta
      901 cgtaacgatc ttttaactga ttaatatttc cccaactttc agcgccccat aaagatatat
      961 gtgctgacca tgaatatctc ttaagttcaa cataaatgtt tccattatca tattgatata
     1021 gccatttatt tgaaaatgaa aaatgaggct gtgtgtaata tttaattaat tcattgatgt
     1081 tagtttcatc ttgaccaatg ctataagctt tagcttcaga gtaaaaacta aatactggtt
     1141 tttgattttg aggtaataca gtacctaata gtaataatgt tgtcgttaaa attatatttt
     1201 tcgtgatgtt cttactcatt agaacatctc ctttcagagg aatcatgata cgaggaataa
     1261 gaaatttaaa tgtgagcgaa gtcaatatag tatttgcgat tatttttatt aactccatta
     1321 ttattgttag tttgattttt cgaggataac ttcaattttt gcattttgag gttttttaac
     1381 atatctattt gcatcagttg atggcaacct tttacttaaa tctattgtgt agttattgtc
     1441 tgcacctgtt attttaattt gtcctttatt ataagaatta ttatataatt ttttactttt
     1501 aattaatgtt tgacgaatac gaaaatctaa ttcttttaaa gttaaaacag gcttattgcc
     1561 ttcataaact ggaaatccgc cagtaaacgt ttctgcttta tctttatatg ttacattcag
     1621 tttatagtgt ttatcgttag atgttgctgc aggagtaaca ccaccagtaa acgtttcttg
     1681 agataatgca aaagaatcaa tggtttcttg gtcttttatg ccaaaaatat caacgctttt
     1741 atttcttaat tggttgatat tgccccaact ttcaggtcca taaacttgaa tatgactata
     1801 ccaagaaaac tgtaacaacg ttgcatgaat cgtaccgtta tctttttgcc ataacgtact
     1861 gttagagaag gttaaatatt tttgcgagta atatttagtt aactcattaa cgttagtttc
     1921 gttttgattt atataataag ctttcgcttc agatgaagaa ttgataggtg tattaggaaa
     1981 ttgtgtagat gctgtaccta atagtaacaa tgttgttgat aaaataattt ttttcgtgat
     2041 gttattgttc attcgaattt ctcctttgag tattgttgga atgtttaatt ataaaaaggg
     2101 gttaattaga taattgaaat tatccgcatt tacaaaaggt aataggttag ttagattttt
     2161 cgagtatgac ttcaatttct gcattacgag gatttttaac ataacggttt gtgtcagtta
     2221 attttaactt tttacttaaa tcaatcgtgt aattatttcc atcagcagtt atcttaattt
     2281 gacctttatt aaattctccg ttatataact ttttattttt tattaatgtt tgacgaatac
     2341 ggaaatctaa ttcttttaaa gttaatactg gtttatttcc tttgtaaaat tcatgtccac
     2401 cgatgatagt ttgttgttta tcactatatt ttaaaaatag tctataaggc ttatcagatg
     2461 aagtagctgc tggcgtaaca ccaccagtaa atgtttcatc ataagtccag taaccttcaa
     2521 ctgtgtcctc atctttagtt ccaaatatat caacgtattt atttcttaac tgattaatgt
     2581 ttccccaact ctcggatcca aacacttgaa tatgactata ccaaacccac gtttgcaatg
     2641 ttgcatgaat gctaccattg ggcttttgcc ataaccattt tccagataat gaaaaatgcg
     2701 gctgagtgta atatttgatt agttcattga tattagtctc gttttcactg atattatagg
     2761 cttttgcttc agatgaaaaa ctgattggtg ttttaggaag ttgtgttgat gtagtttcta
     2821 aaagtaacaa tgctgttgat aaaactaatt tattcatgat gttctttttc atatgaaaat
     2881 ctcctttgcg tgaattaccc aaagtatata agctattaca ccgattcgga attaaataaa
     2941 agctaaaact atgttaaata aacttaaaca gttagtagtg ttatttaagc aaaacttatc
     3001 atttttaagt tttggacaga aacagtatct aataaagtag gcgggagtta tatattaaaa
     3061 acgatacgtt agaattattc tctaatcaat tgtattaaat tgtttgataa ttgaattttc
     3121 taactacttg aaaaatagtt atactttaaa tgtagtactt attttaatta tttcctacta
     3181 cttaaattta atattaataa aatgttcatt taattattga taaaatatta caaattttaa
     3241 tagtaggttg tgtttatttt gtatgcgctt acaatttagg tgtaactaaa ataaaaggag
     3301 ttgttattaa tgaaaaattt acgaaacaga agttttttaa ctttattaga cttttcacga
     3361 caagaggtag aattcttatt aacactctcc gaggatttaa aacgtgctaa atatattggc
     3421 actgaaaagc ctatgttaaa aaataaaaat attgcactgt tatttgaaaa agattctaca
     3481 agaacgcgat gtgcatttga agttgcagcg catgatcaag gtgcaaatgt aacttattta
     3541 ggcccaactg gatcacaaat gggtaaaaaa gaaacaacta aagatactgc acgtgtgctt
     3601 ggtggaatgt atgatggcat tgaataccgt ggtttttcac aaagaacagt agaaacttta
     3661 gctgagtatt caggcgtacc agtgtggaat ggtttaactg atgaagatca tcctactcaa
     3721 gttcttgctg atttcttaac agcaaaagaa gtcttaaaaa aagattatgc agatattaac
     3781 tttacatatg ttggagatgg tcgtaataac gttgcaaatg cattaatgca aggtgctgcc
     3841 attatgggta tgaacttcca tttagtttgt ccaaaagaat taaatccaac agatgaatta
     3901 ttaaatcgct gtaaaaatat tgccgctgaa aatggtggca acatattaat cacagatgat
     3961 attgaccaag gtgtaaaagg ttcggatgta atttacactg atgtttgggt atcaatgggt
     4021 gaacctgatg aagtatggaa agaacgactt gaattattga aaccatatca agtaaataaa
     4081 gaaataatgg ataaaactgg taatccaaat gttatttttg agcattgctt accatctttc
     4141 cataatgctg atacgaaaat aggtcaacaa atttttgaaa aatatggtat tcgagaaatg
     4201 gaagttacag atgaagtatt cgaaagtaaa gcttcagttg tattccaaga agctgagaac
     4261 agaatgcata caatcaaagc agtcatggtt gctacattgg gtgaatttta aatgatataa
     4321 ggaagtgaat atgatggcga aaatagtagt agcattaggt ggtaatgctt taggaaaatc
     4381 acctcaagaa caactcgagc ttgttaaaaa tactgcgaaa tcattagtag gattaataac
     4441 aaaaggacat gagattgtta ttagtcatgg taatggacca caggttggaa gcattaattt
     4501 gggacttaac tatgctgcag aacataacca aggtccggca tttccatttg ctgaatgtgg
     4561 cgcaatgagt caagcttaca tcggctatca attacaagaa agcttacaaa atgaattgca
     4621 ttctattgga atggataaac aagtggtaac actagtgaca caagttgaag ttgatgaaaa
     4681 tgatccggca tttaacaatc cttcaaaacc aattgggtta ttttacaaca aagaagaagc
     4741 tgaacaaatt caaaaagaaa aaggatttat atttgttgaa gatgctggaa gaggatatag
     4801 acgcgttgtt ccttcaccac aacccatctc tattattgaa ttagagagta ttaaaacact
     4861 tattaaaaat gatacactcg ttattgctgc tggtggtgga ggtataccag taattagaga
     4921 gcaacatgat ggttttaaag gtattgatgc agttatagac aaagataaaa caagtgcgtt
     4981 gttgggtgct aatattcaat gcgatcaatt gattatttta acagcaattg attatgtata
     5041 tattaatttt aacactgaaa accaacagcc tttgaaaaca acaaatgttg atgaattaaa
     5101 acgatatata gacgaaaatc aatttgcaaa aggaagtatg ttaccaaaaa ttgaagcagc
     5161 catatcattt attgaaaaca atccaaaagg aagtgtgctt ataacatcat taaatgaatt
     5221 agatgctgcc ttagagggta aagtaggtac tgtgattaaa aagtaattga attgaaacgc
     5281 ttttcaatta ctatatgtca aatgcatgat ttttattatt tatgtgcacc ccctgaaaat
     5341 aatgcctcta ttttgatgcg gggtgcactt tcttaattta tatttataaa atctttaagg
     5401 tagaaatgct aggttaaatg tcgaaggaga tgaaaccgtg gaaaatacaa ttaatgaaag
     5461 tgaaaagaaa aaacgattta aattaaaaat gccaggtgca tttatgattt tattcatttt
     5521 aacggttgtt gcagttatag caacatgggt tattcctgct ggtgcatatt ctaaactttc
     5581 ttacgaacct tcatcccaag aactaaagat agttaaccct cataaccaag tgaaaaaggt
     5641 tccgggtacg caacaggaac tagacaaaat gggggttaaa attaagattg aacaatttaa
     5701 atcaggtgca attaataagc cggtatcaat tccgaatact tatgaaagat taaagcaaca
     5761 tccagctgga ccagaacaaa taacaagtag catggttgaa ggtacgatag aagcggtcga
     5821 tatcatggta ttcattcttg tactaggggg acttattggc gtagttcaag ccagtggttc
     5881 ttttgaatcg ggattgttag ctttaacgaa gaaaacaaaa gggcatgaat ttatgctaat
     5941 tgtgtttgta tcaatactaa tgattatcgg cgggacatta tgtggtattg aagaagaagc
     6001 tgtagcattt tatccgattt tagtccctat atttatagcg ttaggatacg attctatcgt
     6061 ttcagttggt gccatattcc ttgccagctc tgtcggtagt acattttcaa ctattaaccc
     6121 gttctcggtt gtaattgcct ctaatgccgc tggtacaact tttacggatg gcttgtattg
     6181 gagaataggt gcttgtattg tcggtgcgat ttttgttatt agttatttat attggtactg
     6241 taaaaaaatt aaaaacgatc ctaaagcgtc atattcttat gaagacaaag atgcttttga
     6301 acagcaatgg tctgtattaa aagatgacga tagtgcccat tttactttgc gtaagaagat
     6361 aatccttaca ttatttgtac taccatttcc aattatggta tggggagtta tgacgcaagg
     6421 ttggtggctc
//
LOCUS       DANJFZ010000133         6212 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.133, whole genome shotgun sequence.
ACCESSION   DANJFZ010000133 DANJFZ010000000
VERSION     DANJFZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.133"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(169..1539)
                     /locus_tag="PWN45_002411"
     CDS             complement(169..1539)
                     /locus_tag="PWN45_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDL0624151.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(1861..1956)
                     /locus_tag="PWN45_002412"
     CDS             complement(1861..1956)
                     /locus_tag="PWN45_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624152.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(2032..2637)
                     /locus_tag="PWN45_002413"
     CDS             complement(2032..2637)
                     /locus_tag="PWN45_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDL0624153.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(2700..4580)
                     /locus_tag="PWN45_002414"
     CDS             complement(2700..4580)
                     /locus_tag="PWN45_002414"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDL0624154.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            4775..4867
                     /locus_tag="PWN45_002415"
     CDS             4775..4867
                     /locus_tag="PWN45_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624155.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(4975..5472)
                     /locus_tag="PWN45_002416"
     CDS             complement(4975..5472)
                     /locus_tag="PWN45_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL0624156.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(5482..5742)
                     /locus_tag="PWN45_002417"
     CDS             complement(5482..5742)
                     /locus_tag="PWN45_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624157.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(6124..>6212)
                     /locus_tag="PWN45_002418"
     CDS             complement(6124..>6212)
                     /locus_tag="PWN45_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624158.1"
                     /translation="NIGGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT         1924 a         1202 c          846 g         2240 t
ORIGIN      
        1 ctgttaacta aaacgtcaca aagttattaa catttagtga aattcatatt tctacacaat
       61 tatttcatta atttttaaaa ttgtttaaag attcgtccgt catttcgaca tgtttatgca
      121 actattattt cattttgcaa cgtgaccata gtttctattt tttcatactt aaatcgtcac
      181 aatgcccatc aacattgcaa ttactaacat aacgatagcg aatccccaaa tccaaaagaa
      241 tgcatactta atatacgtgc ccatgtttgc ctctgctaaa ccaattgcca accataaggc
      301 tggtgaaaat gggctgacaa atgtacctat aatattccct atgaccattg aataagctgt
      361 tgatacagac ggtacaccaa attgccctgc tgtttgttcg acaatcggta acacagcaaa
      421 ataataagcg tctgtgctag ttagtaaatc taatggtacg ccaagtaaac ctacaataat
      481 atgcaagtat ggtcctactt ctgcaggaat cactttgatt aaattggtcg caatcgcttt
      541 aagcataccg gtttcattta gtacacctaa aaacatacct gctgcaataa tcactgcagc
      601 catcattaat gcattcggcg catgcgctct taatcgttcc atttgttcat ccactgattt
      661 aaaattaata acaagtgcca acgaaacacc tatcatgaat gcaaattcag gtggcgcaat
      721 atttattaac atcgatagaa taacagctaa agttaaagct gtattcaccc attttatcca
      781 tgattttgtt cttgcacgtc cttttacagg aaaccttaca tcttgatctc gttcatatac
      841 ttcaactaat ttatgtacat ctatatcttg tgtttgcggt aattcatttc tctctattgc
      901 ttttttgata cgtttctgtt ctttaaatcc aagatatacc gcaaacaaca taacaagaat
      961 gaaacctatt atttgaatag gtattaatcc ataccataat tcattgacac ttttggcttt
     1021 taacactgca gctacacgag ccattggacc tccccaaggt accatgttca taatcgccgc
     1081 gcttaatgct aatagtaaaa tcaataaata tttattcata tttaacgctt tatataaagg
     1141 taataatgca ggaatagaaa gcaaaaatgt taccgcaccg gccccatcta attgggctat
     1201 tgtgccaatt aaagctgtca ttgcacagac aatgacgaca ttgcctcgtg tcattaatat
     1261 taagcgtttg acaagcggct tgaataaacc actatcgttc atgatgccaa agaaaataat
     1321 ggcaaagata aacataataa caacgttgat gacttgatct aaccctttag caaaaaatcc
     1381 aaccaaatct gtcacactat atcctaaaat cattgcccct aagcaaggta tgattgtcat
     1441 acctacaact ggatttatct ttttggcaat gagtaaacct acaattgaaa taataataat
     1501 gagccccatt actgttaacc acatattatc actattcata ttattttctc cctccacttt
     1561 taatacaatt tacataatcc ccctctttaa cttaaatatt ataattaata taatttttta
     1621 tatttaagtt atctcatttt atcaaaaaaa tcttttttgc gtaactatca atagtgaagt
     1681 ttgattttcc tacttgataa catggcatga ctaatccttg tatatcctgt cctgtatttc
     1741 attttgatct atcattttta ttattgcttt ctattgcaca aaaaatctgg ctaaaggtcg
     1801 ctatgatagt aacgacttca accagatttc aatttatcca cctattttat taagtttgtt
     1861 ctacgtatct ttttctctat tgtaatattc atatttaaaa cgcatatcat tatcgcttaa
     1921 atccttcatt attagactat tcacaaagcc gcccatgtct taatttcaat gattaagcgt
     1981 taaagttaaa ataagaagac ttaccttaga cggttagacg gtccgctatt attatcttac
     2041 atccaaccac gctaaaaatt gagcttgttt gtcatgattt aaatattctt gtgacacaat
     2101 ttgtgacagt aactgttgct tttcgttata tgttatatca agtactttta ttttctgtcg
     2161 gcaagtatat aaaaagtcga tatacgaact gtatgatggt ggatataacg cctcaagctc
     2221 tgccataatt gattttgtca acttaggact cgcaccatca gttgatacac tgatagttag
     2281 cttgtcgcgg tgtagtgcac ttggaaatac aacattgcca tttgatgcat ctccaacatt
     2341 attaaaaagg gcatgctcag gtaaggcttt ttttaccgct tcattgacac gtggctcatt
     2401 ggttgctgca ataactagat acgcgtctac aatatcgctt ggttcaaatt ctttttcttt
     2461 ccatatgacg acaccgttat ctaccatatt ttgaagtttt tcagtgattg tcggactgat
     2521 gaccgtcata tgttcaacgt attgatttaa tgtttgtgcc cgacgacttg caacgacgcc
     2581 tccaccaact atgacgacat ttttatttgt taaatctatc attaatggca tattcatgtt
     2641 gagcaacatc ctttatatct ttttacatca ttacacctta aaatatttaa caccaatcgc
     2701 taataaacat cacgttgata gcgttgttgt tgtttcattt gtcgcaataa taactctgct
     2761 tcttcttgag aaatatgacg ttcttttacc aatacatctt taatggcttg atggacatct
     2821 ttcgccatac atttttcatc gccacaaata tagattgctg cgccttgctc aatccattca
     2881 ttgaaacgtt tactttcttc cattatacga tgctgtacat attctttgtg ttcttggtct
     2941 cttgaaaatg ctaaatctac gcgtgttaag tttccatttt caagccattc ttctatttct
     3001 tcttcatata aaaagtcaga actacggtgt tgatcaccaa agaacaacca tgtttttcca
     3061 gtcataccaa gttcttcacg ttcttgtaaa tatgctctaa aaggagcaat tccagtacct
     3121 ggtccaatca taataaccgg tatatcttgc ttcatcggaa atttgaagtt cggatttttc
     3181 ttcaaataaa ttggtactat atcgcctggt ttaattcgct cagcaaaatg aaccgagcat
     3241 acaccttttc tctcacgtcc atgtgcttga taacgaaccg taccaacggt aatatgcact
     3301 tcatctggcg ttgccataaa actactagaa atcgaatact ctcttggtgg taattttcta
     3361 aggatttgat acatattctc aggttgtaat tctatagttg gaaaatctgt tattaaatct
     3421 ataaagtccc gatttataac atattcacgc gcccatgact catcttgaat acgttcagat
     3481 aattcttcat tgtcaaaata gatatctgca tttttcaata atggcaatgt taatttagta
     3541 aattcaaaat gtgatgttag tgcttcaaca ataggaactg tatcaccatg atcattaatt
     3601 ggcaccggag attgcggatc ccaacctaac atgaatatta gtttttcaac caattcaggg
     3661 tcgttttgcg gtaatgctac tatacaatct cctggttcat atgattcact aaaatcatca
     3721 agtaaaaatt ctatatgtcg tgtttcttta tttgaatcgg taccatttaa attgatattc
     3781 gctaatactt ctgcttggta tggatttgat ttagaatatt tcttttcttt ggcagactta
     3841 attgattcac ttatcaccga ttcactttga ataccttctg atgtggtatc aataatatta
     3901 atgatgtctg ccatccactt ttctgcgtct tcttcataat caatatcaca atctacacgc
     3961 ttacatatac gctcagcgcc tagattttct agtaaaacat ctacatcttt accggcttga
     4021 cagaaaaatt catatgtttg atcacctaaa gctagtactg aatatctcac atgatttaaa
     4081 ttaggtgcgt tatcgtcttc taaaaattca aagaaatccc atgcattatc aggcggttct
     4141 ccttcaccat gagtagacgt aataataaat aaatcttcta actgcgcgat gtttgtcgta
     4201 tcatattcat ccattgacat caaaacaact tgatgtccga tatcacttaa acgttctgaa
     4261 aaaatttcag ctaaacgcat ggcattacct gattcagaac catatagcac tgtaacatgc
     4321 cgctgattag cttcgatatg tggctcttta ttttgtaaca tatacgttgt tgaagttgct
     4381 tgttcttctg atatttgtga ttgttgccac acttctgtct ctaattgttg ttgattacta
     4441 tcacttgtcg tatcgttact attagctagt aaaaaaccac taagccactg ttgttggctc
     4501 tctgttaatg tttgcagcag attgttaatc tctgtgactt gtttttctgt aaatggacta
     4561 ttagatgtat ttaatttcaa atgttttcac ctcaataaac ttgaaataga tgattgactc
     4621 tttttttatc gatagtttag tcaatttttt aatatgtatg gtataaatta aaacaaactt
     4681 gagttgtgtc tgatagttat acttgcaaat atttacaacc ttcatgttgc ttgaatgaca
     4741 ttatcagtaa ttcagacaaa actttgatta tgtcgtgtca gttattaatg catacagtaa
     4801 tgcatcgttg ttatttttta tttatttact atttattatg taccaaatca gcctttcagt
     4861 aaaataggtc aaattactga ttttctaaat ataaaatgcc tcctaataac atactattag
     4921 tacatcatta agaggctctt gtgttatttg catactaagc gctcaaattt aaatttaaga
     4981 tgaagattct tgcaataata tttctatatt tgtcgatata tccaatggat cttccattgg
     5041 caagaatcga tttacaacat ttccttgtcg atcgattata aattttgtga aattccattt
     5101 tattggggac ccaaagattc ctggttgttc attctttaaa tgcgtaaata acggatgttc
     5161 atcgttcccg ttcacagata ttttagctaa cactggaaat gttacaccaa atttctcacg
     5221 actaattttc aagatttctt cattagaacc tggttgtcga ttgtcaaaat tattattcgg
     5281 aaaactcaac actacaaacc cacgatcctt atatttttga aataaagtct ctagtttttt
     5341 caattgttcg ctatatatac attctgttgc agtattaaca actaaaatca ctttacctct
     5401 aaatgcttct aatttataag ttaagccttt ataatcactt acttcgatat catacacatt
     5461 tctattattc ataagacacc cctacacagc cttttttata ttgaatatgt tctttttaga
     5521 atgttctgat aaaataagtg cgcgtttaca ccgtgaacac acattatata gcgtgataca
     5581 tttttcgagc acacgataaa taatgttcga gtttatgttg ttgctcaacc tatccgattt
     5641 accgtctttt ttcacctgtc ttactttatt aaaataatca cattgtatag ctgtattagt
     5701 ccagctacaa tatcttccgt tataacccct cattataatc atccttattt tctattttta
     5761 aaaagacaat tagaccgctc tttaaactat agattaatac ttaagttaaa ctcatacata
     5821 ctgataccat acgttagatc taacaattta aaattcgtta taactatgga ttaaagagct
     5881 gcccaactca tataatcctt aaaaacttca catgtgattg tttattaagc cctcctttat
     5941 cttattaaat atccttataa cccttttaaa attaaactga cacactcata cattgttaca
     6001 cgcgacgata aacgtcatat tattgcatat cattaaaaca agtcactttg tatcgtgtgc
     6061 gaccgaatac atattaaatc atcaataatg attattcaat tattaaaaat aaaaacgaac
     6121 attctatata gagtttctac ttattatttt ttcagcattg tctacattac ttaacattcg
     6181 tgtttgtaag taatattgac cgccaatatt ta
//
LOCUS       DANJFZ010000134         6037 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.134, whole genome shotgun sequence.
ACCESSION   DANJFZ010000134 DANJFZ010000000
VERSION     DANJFZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.134"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            92..856
                     /locus_tag="PWN45_002419"
     CDS             92..856
                     /locus_tag="PWN45_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDL0624159.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            869..1321
                     /locus_tag="PWN45_002420"
     CDS             869..1321
                     /locus_tag="PWN45_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0624160.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1463..2947)
                     /locus_tag="PWN45_002421"
     CDS             complement(1463..2947)
                     /locus_tag="PWN45_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0624161.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(3068..3436)
                     /locus_tag="PWN45_002422"
     CDS             complement(3068..3436)
                     /locus_tag="PWN45_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL0624162.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(3569..3949)
                     /locus_tag="PWN45_002423"
     CDS             complement(3569..3949)
                     /locus_tag="PWN45_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDL0624163.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(3950..4606)
                     /locus_tag="PWN45_002424"
     CDS             complement(3950..4606)
                     /locus_tag="PWN45_002424"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0624164.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            4790..5620
                     /gene="thiD"
                     /locus_tag="PWN45_002425"
     CDS             4790..5620
                     /gene="thiD"
                     /locus_tag="PWN45_002425"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL0624165.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         1981 a         1090 c          983 g         1983 t
ORIGIN      
        1 atcaataaca acttatcaaa aagctcatgt ggttatttta tagtgtataa actataatga
       61 gtattaaatt cttataaaca atggtgatga aatggacata aattcagaag aatacaaaca
      121 agaggtactt atcaaagacg ttgtcatgct tgctgctcgc atactattag aatctggtgc
      181 agaaggtacg cgtgtagaag ataccatgac acgtattgca aaaaaacttg gttacagtga
      241 aagtaacagc tttgttacaa acactgtcat ccagtttacg ttacattcgg aatcgtttcc
      301 tagaatattt agaattacct ctcgagatac aaacttaata aaaatttctc aagctaataa
      361 aatttcgcgt caaattacaa acaatgaaat ttctttagcc gaagcaaaaa cgcaacttga
      421 aaaaatatat gttgctaagc gtgacagcag tcttcccttt aaaggttttg ctgcagcaat
      481 gattgcaatg agtttcttat atttacaagg tggtagattg attgatgttt taactgcgat
      541 attagcaggt agtctaggat acctagtcac tgagatttta gatcgtaagt tacacgcaca
      601 gtttatccca gaattcattg gttcattagt tattgggatt atcgccgtta ttggacatac
      661 acttattcca acaggtgact tggcaactat tatcattgcg gcagtcatgc ctattgttcc
      721 tggtgtatta ataacaaacg caatacaaga tttatttggt ggacacatgt tgatgttcac
      781 aacgaaatca ttagaagcat tggttactgc gtttggcatc ggtgctggcg ttggtagcgt
      841 attaatttta gtataggagt atcagactat gttttggatc ttaaacttta tctttagctt
      901 tttagcttca atgttcttct gcgtcatttt tgatgcacct agaaaattat atctatcatg
      961 tggtttcgtt ggtacgtgtg gatggatggt ttacacctta ttcttcaacg gctttaatgt
     1021 gcacactata tactctagtt tctttggtag tttagcatta ggcttgttaa gtcattatat
     1081 ggctcgtaaa caaaaagaac ctgccatcat ttttatggta acgggtatca ttccattagt
     1141 acctggtggc ttagcatacg atgctacaaa aaatttagtc ttattaaatt tcagtacagc
     1201 aatcaatacc atgctagagg ttacacttat tgcaggcgcc atcgcattag gtttattatt
     1261 cgccgaccaa atttccaaat taattgtttc tgggttcgtg aaatctttta aacgattata
     1321 aatatttgtt agaaatggct cagccctaac tcttagcaat tatatataag gttcagctgc
     1381 ctcgtcttaa atgtcaattt ataatacaaa aaatcgcacg atacgcctcc gaatgaagaa
     1441 ggtttattgt gcgattcttt tattatttag ttttttcttt ttcaggttct tcaatcatat
     1501 catcaacttc attttcactt ctatttaaaa tcaagcgact taattcatca ttatccatat
     1561 ttttcaattt aggatatcga atcacaaaga atattaaacc gatgattaac catccaagta
     1621 atgcaatata agacggtgca gtcagtgctg caggagaacc tggcactaat aacaacgcta
     1681 agaaaatgaa tgatacaaat gagccgataa tagcaaacgt tttgtaaacc ggtgcatacg
     1741 tattactttg tttgttataa ctgaataatt tcgctgcaga caaacatgta ataaagtagg
     1801 caatggatac accagtagat gacatatcta caatccaagt caatgcagtt cttcctagcc
     1861 aaggtgcaat taacgacact cctactagga atatgattgc gacatatggt gttttgtatt
     1921 tactatgtaa tttactaaac attgttggca taatacctga acgtcccata gaaaataaca
     1981 agcgacttga actcatcaag aatccattta aaccagtaaa tatacccatc ataattgcaa
     2041 ttgctaatac acctaatcca atataaccaa atgctgtttg tgtaacagca cctgttaacc
     2101 acaactgccc atttaaactt tgatgacttg ttgataacca accagtgtat aaaatcatga
     2161 caacataagt taatgatgct gctaataaac tgtacacgat aagcttaaat gtcttgtttg
     2221 gtgcaaagtt aaactcttct gctgtttgtg gaatattatc aaatccaaca tatgcccatg
     2281 gtgccacgga tacaataacc acaatagaca ctaaccatcc tttgctaggt tcagctaacg
     2341 gttgtaaatt ttcaagtgca aaattattac caaagaatga accaaagaac atcaataata
     2401 cgacgattac catcgccaca cagaaataat attgtaatga tccagataca cttgcgccac
     2461 gaatcgttac tagcatgaat acaagtagta atacggtcgc aataatgatt tccgtaatat
     2521 aaacgtccca gcccgcaatg gtgtatagtt tcccattatt taagacatct ggcaataaga
     2581 atttaactag taaactgaat gcggtcgcat ttaaagcaac gacacagaca taaccaaaag
     2641 ttaaaaacca tgatgagaag aaactcacat atctgccgaa acttaagaaa ctaaaggcaa
     2701 acgcgccccc tgatactgga aatctctcta ctaatgcgcc ataactaacc gcaattaata
     2761 tcattaataa tgcaccaata actataccaa ttgatgctgc aatcggacct gactgcttaa
     2821 tccagtctcc tggtaagatg aatgcgcccc atccgataca tgaaccatat gcaatcgccc
     2881 atacaaactt ttcagatagg ttttgtttta aatcgcctct atctatttgc ttattctttt
     2941 tttccataaa taaaaactca cctcgaaggt attctatacc caaaagatga gtttgtaact
     3001 ctttttcttg attattttta ttaaaaattt aatgtttagt tacataggta atctaaagtt
     3061 ttaaaattta accagcaaat ttgaatgtcg caatgattaa cattatccat ccaatgatga
     3121 acaatacgcc accaattggc gtaatcgcac ctaaaacttt aatttgagtt aatactaaaa
     3181 tatataatga tccactaaag aaaataatac cagcaaatat taaccagcca gcccagttaa
     3241 cattgattga agttgtacca ctaattacac ctataattaa taatgctaag ccatggtaca
     3301 tttgatacgt cgttgctttt tcccatactg ataaatagtg atcacttatt tttccttgta
     3361 aaccatgcgc accaaatgca cctgtaccga cagccatcat cgcgtttaat gcacctaaaa
     3421 taataaataa tttcattgct acctctcctt atataattca gtattaataa atgtttaaat
     3481 ccgaacactc gaataaaagt atatgcgtct tgattattaa cctacatact taacaagcta
     3541 ttaagtaatt atgtttcatt gttgcttttt aaaaatcaaa tatggattca ccattgccaa
     3601 tgtcatcatc tgtcgctaat ctttgttgtt ggtttgaagg ttgtgaatat gacttattat
     3661 gatgatgtgc tgaatgcgta tttactttac cacccatcgc ttcaatttca gcaactgtca
     3721 cttgatgagt tggctgcgtt atttgtgatt gtggcatttg attatggtta gcaatacgac
     3781 gattcatttg ttgttcacca atatgtggtg tatttgatgt tgaagcatat aaagatgtta
     3841 atatatgtat ggcatacata tgtttttcaa agtcatggtc atgctgtgcc tgctctgctt
     3901 gtactaattc ttgctcaatt cgtgctatta aagtttctct attcaacatc tacgcctcac
     3961 tttcacacca attaattggt gattttccta ctgactctaa ataggcattc gctttggaat
     4021 acggttttga tccaaagaat cctctatatg cagacagtgg actaggatgc actgatttta
     4081 taatacaatg tttagatgta tcgataagct ttattttttg ctgtgcaggt ttcccccaca
     4141 aaataaagac aacatgttct ttataatcag acactgcttt aataatttca tcagtaaatg
     4201 tttcccaacc aatatcacga tgagaatttg cttcaccctg tcttacggtt aaaactgtat
     4261 tcaataacaa gacgccttct cttgcccaat cttgtaaatg cggtgtttgt ctaacgcatc
     4321 caatatcatc tgctaattct ttatacatat tacgtaaaga tggagggaat tttgcgttag
     4381 gttgcactga aaatgctaat ccatgtgctt ggtttggacc atgatacggg tcttgtccta
     4441 atataacaac tttgatattt tcaaacggtg ttaaatcaaa cgcttgatat atattttccc
     4501 tatcagggta tacgattgca gtcgaatatt ctttttctaa aaaatcatgc atagctttaa
     4561 agtcatgttt cgttgttatg tcatgaaaaa tttgcgacca ttccattcaa tctcacctca
     4621 ggacatattt taacatatca cacttatcta aagtgaatga cattttcaaa agttacaata
     4681 gtatgaatga ttcatttcaa caatcatcaa atattgaatg cttaatattt ttcaattagg
     4741 ttaaaaatga tatgatgaat acaatattat tatgtaggag tgaagctcaa tggctttaaa
     4801 gaaagtttta acaattgccg gttctgacac aagtgctggc gcaggtatgc aagcagattt
     4861 gaaaacgttc caagaattag atacgtatgg catggtcgct ttaactgcca tcgttactat
     4921 ggataaagat acatggtcac acgatgttac accattacca atggatgtat ttgaaaaaca
     4981 acttgaaacg gctttatcaa tcggacctga tgctattaaa acaggtatgt taggtactga
     5041 ggagataatc aaacgtgctg gagaagtata tgaggcatct aatgcacaat attttgtagt
     5101 agatcctgtc atggtttgta aaggcgaaga tgaagtgctt aatcctggaa atactgaggc
     5161 gatgattaaa tatttactac ctaaagcaac ggtagtgaca ccaaacttat ttgaagcggg
     5221 tcaattatca ggattaggta aattaaattc aattgaagat atgaaaaagg ctgcaacaat
     5281 tatctttgat aaaggcgcac agcatgtcat cattaaaggt ggcaaagctt tagatcaaga
     5341 taaatcgtat gacttatact atgatggtca aacattttat caactaacaa cagacatgtt
     5401 ccaacaaagt tataaccatg gtgctggttg tacatttgct gcggcaacaa ctgcatattt
     5461 agctaacggt aagtcaccga aagaagctgt gattagcgcg aaagctttcg tcgcttctgc
     5521 tattaaaaac ggttggaaaa tgaatgattt tgttggtcct gtggatcacg gtgcatacaa
     5581 ccgtattgaa catatcgatg ttgaagtaac agaggtttaa cgattaggcg gttcatctat
     5641 aaatcaacat tattagccac accacttaaa ttgggtatga actcaattta tgtgatgtgg
     5701 ttattttttt ataatcatca tgctctgtat ataatttaaa tttgaaagtt acatataagc
     5761 gttattagct tttattgaaa atttcataca tatcgaacaa tacatacaaa tatttcctat
     5821 acatctacat ctatgactaa ataacccttt attttcaaga tgaacttaaa ctcatctata
     5881 tgctcaatac ctctatcatt atcattcttt tgcgatagat gcctctctaa tcatatatgt
     5941 tatatttccc aaatgactgc caggcgactc tataaatata tatttattta gaacttttat
     6001 acataaatag tcttagtaaa tcatacgttc tatgtct
//
LOCUS       DANJFZ010000135         6034 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.135, whole genome shotgun sequence.
ACCESSION   DANJFZ010000135 DANJFZ010000000
VERSION     DANJFZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.135"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(905..1099)
                     /locus_tag="PWN45_002426"
     CDS             complement(905..1099)
                     /locus_tag="PWN45_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624166.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1209..1901
                     /locus_tag="PWN45_002427"
     CDS             1209..1901
                     /locus_tag="PWN45_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624167.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(2069..2218)
                     /locus_tag="PWN45_002428"
     CDS             complement(2069..2218)
                     /locus_tag="PWN45_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624168.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(2765..3081)
                     /locus_tag="PWN45_002429"
                     /pseudo
     CDS             complement(2765..3081)
                     /locus_tag="PWN45_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(3074..4057)
                     /locus_tag="PWN45_002430"
     CDS             complement(3074..4057)
                     /locus_tag="PWN45_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0624169.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            4483..5226
                     /locus_tag="PWN45_002431"
     CDS             4483..5226
                     /locus_tag="PWN45_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0624170.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(5453..5638)
                     /locus_tag="PWN45_002432"
     CDS             complement(5453..5638)
                     /locus_tag="PWN45_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624171.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5643..5981)
                     /locus_tag="PWN45_002433"
     CDS             complement(5643..5981)
                     /locus_tag="PWN45_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624172.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
BASE COUNT         2259 a          865 c          828 g         2082 t
ORIGIN      
        1 tcggcataaa tgcgtgtcta tgcgtagaca tggagacact tcagaattat ggagtgatta
       61 catgagttta actatttttc tttattcagt tttaggaagt ttattagcaa attatatcta
      121 cgaaaaatct tcaaaataaa taactttttc ggttctactt tgtacacttt atctacctgg
      181 tagacctaat tgttattgct ttacaggtct aaaattcgtc gttataatct agttaaatcc
      241 cgtaagggcg cacttatacg tcggataaat cgacatgtgc aaaaacgtaa tcgaccttcg
      301 tcgatttttt tatttcaaaa ttaaagaaac ggcttgagat attccgagca gttataaaaa
      361 ttaatttgcg ttgaatattg ctactacatt cttgaaaaag tgatgtagat ttatagccca
      421 taactttttt gaattacatc gacgatacgg cgtcgaaaga aaaataaata gaccgtatga
      481 aagaaaaagc tacctcactt tatctggggt agcttttatt ttttatgccc ttattttgaa
      541 ttttaagagg gcattttaaa gatttagggg tgattcatat agttttatat ctaaaactta
      601 tatatgcttt taaaacgcaa atatgagcca aataaatata tgctattttc aaaaacaaaa
      661 atttgagcaa aaacaataac tatttttaga cactgcccat ttacatgcaa attaaaaatt
      721 agcataaaat tctttaaaac ttccccacgt ttttctaaat tcctgtaggg cgtttttatc
      781 aaaaatcaag acaagaagaa actcgttcac actcgtttca aaaaaacaag aaatctaatc
      841 tatacaccta ccgttttcca accccctcaa agcatacaaa ttaaaaacaa aattattcat
      901 aatcttactt tcttttaaca aaaacataaa aaccataaat agacaaatta actatcaaag
      961 gcaataaaca gtataaaaaa accacgaata aatcttcatc aacatctaaa ataaaaaaga
     1021 ataaaacaat taacaaaata ctaattaaca aacctaaaag tggcaaactt tttatatatt
     1081 tttctaaatt ttcattcata aaatagactc ctttcaaaaa tatgtttata aatatattaa
     1141 aataaatatt gattttataa agcgcttact tatacaatta aaatatctta ttatcttgga
     1201 ggtatcaaat gaaaattata aaattatttt caataatttc cttgttccta ctggtattta
     1261 gcgcgaattt ttctaatgct tacgcaaatg aagaacaaaa aagttcttta ctagaaaatc
     1321 aaaaagaaaa attgaaaagc aaaaacatcg aagtatcaca aatgaccaag aaagaaataa
     1381 gttttctaga acaattgcaa aataaaaatg aagatatgca aaaattcgaa aagcaaatga
     1441 aaaaagaagg atttaaaaaa ataaagctag aagacaaaaa ttctaaatta aaatatctag
     1501 ctacagaagc aaatgataaa aatgcatatg gctacatagc taataacgct tataaaaatg
     1561 aaaaaacaaa agaaattgta gtttctgaaa caatatatga taactacaac aaaaaaatca
     1621 caaaattcat tgccgaaaaa agaagtatga ctgaaaacaa agagggaaaa atacttatta
     1681 acaaaaatta cgttgaatat aaggctgaaa aagttgataa aaacagaccg gtaacatatg
     1741 caggattcaa atggaacggc aaagcattcg cttgtagtat ggctggactt tatgcatgtg
     1801 ctcactactg tggagtatgg gcaatcgtaa atccaatagc cggcggtgta tgtgaagcag
     1861 tttgcggaac tgcatttgca gctggatgct ctgtaggttg attttattcc aattgtttta
     1921 ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata
     1981 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata
     2041 aattttttcg gcataaatac gatgttcctt atttatcctc ataaaaaaca atagctgaaa
     2101 tatataacca attactgtgt gtttgaattt ctaatacatg aatatcattc tttaaaaacc
     2161 tattaacttg tttatctaat tgacgatgag taaaagtagc gaaagtttta atcttcatga
     2221 tatctctcct tttataaaaa tagcaatcct tattacaata ttaaacactt ttattttgga
     2281 ttttaagagg cattttgaag acttagaggc aaatcatata ggttttgtac ttaaaaatct
     2341 atacagtctc ttaaaacgca aatatgagcc aaataaatat atgctaattt cacaaacaaa
     2401 aatttcagca aaatcagtga ctaatttttt agatactgcc catttacatg caaatttaaa
     2461 aattagcata aaatacttta aaacttcccc atgtttttct aaattcctgc agggcttttc
     2521 ttatcaaaaa cttagaaatt ttaatttaac tctaaaatct ccgtaagagc ataagggaaa
     2581 tttgctatca ttcaaaaatg atagatctgg caaggccaga cattccaagg gttttaagtt
     2641 attttaaagt gatatcatac aaagctgtag tgtcacactt ttgtgacact acatacagca
     2701 tttgtcacaa aaataagaca gcactttttt acagaaaaac aataaaaaaa gacatcagtt
     2761 atcactaatg tcttaaataa taattttgct tttcgaaatt ccaaattgcg gtaattgaat
     2821 gtgctttttt tcttcgtctg tcgttttatc ttcatcactt gtattaatca aattaccatc
     2881 ttcggcatcg tctaagttta aaattttatg cttttgttta agcaatccac cataacttaa
     2941 catacgtttt cgatacaaac ctttttctaa atcacttaca atttcttgat ttttttcgtc
     3001 gtcatcagtt aaaaagtcag atgatttaac ggaatatttt gatgtctctt cgagtgttat
     3061 aaaatcattt gaattattga ttaaagatat tatattgttt catgttttta cggcgcatat
     3121 aaagggcatg tgtttcttca agttcagtta gaattgtttg cattaaataa gcttgcattg
     3181 acttgattgt ttccttttgg cctttaactc ccctatcatg tatagctttt ttaagtcttt
     3241 ttaaagaagt taatatttca tcctcaatgt cctctaacat gtaatgcata tcttcatgcc
     3301 ttgaattaaa tgattttttt gctttaagaa taacagcctt tatgatttct aaatctctat
     3361 aatcatattt taataaataa ttagttagtt gttctgggaa ttcttgcaat agcatttctt
     3421 tatcatcaaa attatgtgta aattgatttg aatgatttga atgattacta atttcatttt
     3481 tgtactttat atcattcaag tcattcatat cattattctc agtatcatta aagtcaatgt
     3541 cattaaagtc agtatcatta gtgttcgatt ttcgaacttc tggacgttcg attttcgaac
     3601 ttctggacgt tcgattttcg aacttctgga cgttcgattt tcgaacttcc ttctcgccgt
     3661 aaggttcaac atcgttttct tctttttcta ttttgtaaat atcacgttct gtgacaatag
     3721 gtttcattaa atatagaata tttggtttat ttaaaccacg tcgtttttga atgagtagac
     3781 catcgttttc taaccctttt tttatttttg ttaacttttc ttttttacaa tttaagattt
     3841 ccattagctt ttcatttgaa taaacaaagt aaatgttacc atcttcatca acccagctat
     3901 ttttaataga taaatttaag cgatcacgta agatagcata ggctattttc atatcattag
     3961 ttagattttt ataattttca ctggtaaaga atactttagg gatttgataa aatctttctt
     4021 tgtaattttc ttgcactgta aaatattgat tttgcatgac aaaaaacctc tttccatatt
     4081 ttagtttggt aagaggtaat aaaacgtttt tatttagctt tacatttatt taatgattta
     4141 ggtgcttgca ctaagatatt attcattata taatacaagt atcgaaaatt atataaataa
     4201 acagtttcta aacgttttat taccaaaccc cgactattac cagtagttgg ggtttttgtt
     4261 atattaagtt tattatagca ctttaaataa acttataaaa gggaaaatat agctttatca
     4321 agggtttata aacccttgat aaatatcata aacctttcat aaacccttga taaaaaggtt
     4381 gatggatgtt tatttttatt tattaatcac tttattatta agcattataa gcttataata
     4441 ttttatcaag ggtttataaa cattttataa agggttgatg atatgaaaag tgtgaaaaag
     4501 ttgagtgaag aattaggggt tagtaagcaa acgattttta ataatattaa gcgattgaat
     4561 atagagacaa taaaacagga aaacacttcg tttattaaag aagataccga tatagaaaaa
     4621 atcattcaaa gagtaaatga aaataaaaag aaatatggtt ttgaaagtac tactgaagac
     4681 aaacaaaaaa aagggtcgga taatattaat tttgaatcga aaagcgatgc tcaaatagta
     4741 gaaatcctta aaaatcagat tgatacttta aataatcaaa ttgaaaaaca agaaagtcgt
     4801 catgaaacaa caattgaatt ttacagaaaa gagttgcaag aaagatcaaa attgcttgaa
     4861 aatcagcaag ttctggcatt agagagtaat aaaaagattc aaaaattaga aaatcaatta
     4921 gaaaaagaaa aacaccttaa ttatcccttt gatacatcta ctaacactag acaaaacgtt
     4981 gatgcacaag aaaaaacata tactactaat cctgtaaata ttaacagaga tcaggaagaa
     5041 actaaagaaa cagagattca atataaggat atatctggaa gtcaaagcga cgaaagtaca
     5101 caaggtgaag aagcaaaaaa agaagacgtt ccagctaatc taaatgacaa tgatagtgat
     5161 atagaagaaa agagtgaaga aacggaacct aaaaaggggt tttggagtcg tttgtttggt
     5221 agctaaaata attgattagg cactaaaagt tataaaattt tagtgtcttt tatatagtga
     5281 gaacttctgg taatcatcga ttatgtaaac taaagtattt caaataatta actgtattaa
     5341 tactctcatc atgattgcgt tataaacgtt cgttattgag acaaaattta atagtactac
     5401 tacacaaaaa gagagtgcga attcgcactc tctttttgtg ataaatgaat ggttaagaaa
     5461 ataattcatc cacagttgta tcaaacaatc ttgccaactt aaatgctaat gtcaatgtag
     5521 gatcatattt attgttttct attgcattaa tagtttgcct agacactttt aattctttac
     5581 ttaaagtttc ttgagaaatt cctttttgtt ttctatattc tctaattcta tttttcatta
     5641 atttaacctt tagatgtata ttttttattt gtagtatagt aattaaacag cataagaaat
     5701 agtgctacaa ttataaagaa aatttctgga tattgagaaa accaagatcc ttcaatactt
     5761 ggtgacaaac ctgtaaattt aagcactagt gtaaataaca tacaatatac aatccaaata
     5821 aaacttttag ccagtatttc aataaacatt ttacgttgtc tttcatcgta tcgatcttgt
     5881 tgtccttttt tatctaatgt ttctgaaggt aaatatttgt tagttatagc cgtccataaa
     5941 caagaaaaaa ttaaaataat aataaaagga attaaattca ttataacacc tcataaatgt
     6001 aaaaagtatt tgacatttat gagtgtaaat attt
//
LOCUS       DANJFZ010000136         6004 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.137, whole genome shotgun sequence.
ACCESSION   DANJFZ010000136 DANJFZ010000000
VERSION     DANJFZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.137"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            355..1521
                     /gene="aroC"
                     /locus_tag="PWN45_002434"
     CDS             355..1521
                     /gene="aroC"
                     /locus_tag="PWN45_002434"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL0624173.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1547..2611
                     /locus_tag="PWN45_002435"
     CDS             1547..2611
                     /locus_tag="PWN45_002435"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL0624174.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2621..3919
                     /gene="aroA"
                     /locus_tag="PWN45_002436"
     CDS             2621..3919
                     /gene="aroA"
                     /locus_tag="PWN45_002436"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL0624175.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3926..5170
                     /locus_tag="PWN45_002437"
     CDS             3926..5170
                     /locus_tag="PWN45_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624176.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5184..5759
                     /locus_tag="PWN45_002438"
     CDS             5184..5759
                     /locus_tag="PWN45_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL0624177.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            5749..>6004
                     /locus_tag="PWN45_002439"
     CDS             5749..>6004
                     /locus_tag="PWN45_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDL0624178.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLF"
BASE COUNT         2146 a          833 c         1122 g         1903 t
ORIGIN      
        1 aaacggactg attaacttta ttaataatta acagttcgtt cttttgtatt aagaaatgta
       61 gtcagtatat tatttgctaa agttgcgata cgattatatt aaaacggcta atcattttta
      121 attaatgatt atatgatgca actgtttaga aattcatgat acttttctac agacgaatat
      181 attataatta attttagttc gtttaatatt aagataattc tgacatttaa aatgagatgt
      241 catccatttt cttaattgag cttgaaaaca aacatttatg aatgcacaat gaatatgata
      301 agattaacaa catattataa tgttatcgtg gaagtatgaa aggagcgagt gtgtatgaga
      361 tacctaacat caggagaatc acatggacct caattaacag ttattgttga aggtgtacct
      421 gcaaatatag aaattaaggt tgaggatatt aataaagaaa tgtttaagcg tcaaggcggt
      481 tacggacgtg gacgtcgtat gcaaattgag aaagatacag tagaaatagt atcaggcgtt
      541 agaaatggtt atacattagg tagtccaatt actatggttg taaccaatga tgactttacg
      601 cattggagaa aaattatggg agcagctcca ataagtgaag aagaacgtga aaatatgaaa
      661 cgtactatta caaaaccaag acctggtcat gcagatttgg ttggaggtat gaaatataat
      721 catcgtgatt tacgaaatgt gctagagcga tcatctgcta gagaaacagc agctcgagtt
      781 gcagtcggtg ccttatgtaa agtgttatta caacagttag atatcgatat atacagtcgt
      841 gttgttgaaa taggtggaat taaagataaa gatttttatg attcagaaac atttaaagca
      901 aatcttgatc gtaatgatgt tcgtgtaatt gatgacagta tcgcacaagc aatgcgagat
      961 aaaattgacg aagctaaaaa tgaaggagat tcaattggcg gtgtcgttca agttgtagtt
     1021 gaaaatatgc ctgttggtgt aggtagttat gtgcattatg atcgtaagtt agatggtaag
     1081 attgcacaag gtgttgtcag cataaatgct tttaaaggtg taagctttgg tgaaggattt
     1141 aaagcagctg aaaagccagg tagtgagatt caagatgaaa ttctatataa tagtgaaatt
     1201 ggttattatc gtggatctaa tcacttaggt ggtttagaag gcggtatgtc aaatggaatg
     1261 ccaattatcg ttaatggtgt aatgaaacca attccaacgt tatataaacc attaaattca
     1321 gtagacatta atactaaaga agactttaaa gcaacaattg aacgttctga tagttgtgct
     1381 gttcctgcag caagtatcgt ctgcgaacat gtcgtagcat ttgaaatagc aaaagcatta
     1441 ttggaagaat tccaatcaaa tcatattgag caacttaaac aacaaattat tgagcgcaga
     1501 caattaaata ttgagtttta acaacaagaa caattgaggt gtaatcatga aattacaaac
     1561 aacataccct tcaaataatt atccaatata tgttgaacac ggtgcaattg accatattag
     1621 cacgtatatt gatcagtttg atcaaagttt tatattaatt gacgagcatg taaatcaata
     1681 ttttgctgat aaatttgatg atattttatc atatgaaaat gtacataaag ttattattcc
     1741 agctggtgaa aagacgaaaa catttgagca atatcaagaa acattagagt atattttatc
     1801 ccatcatgta actcgtaata cagcaattat tgctgttggt ggtggtgcaa ctggtgattt
     1861 tgctgggttt attgcggcga cacttttacg aggcgttcac tttatacaag tgccaacgac
     1921 tatactagcg catgattcta gtgttggcgg taaagtgggt attaactcaa agcaaggtaa
     1981 aaaccttatc ggtgcatttt atcgtccaac tgctgtgatt tatgatttag actttttaaa
     2041 gacgttacca tttgagcaaa tattaagtgg ctatgcagaa gtttataagc atgcgttatt
     2101 gaatggtgaa tcagcgacgc aagatatcga acagcacttt aaagatagag agatattaca
     2161 gtcattaaat ggtatggata aatatattgc taaaggtatt gaaacgaagc tggatattgt
     2221 tattgcagat gaaaaagaac aaggtgtacg taaattttta aatttaggtc atacatttgg
     2281 tcatgctgtt gaatactatc ataaaatacc tcatggtcat gcagtgatgg ttggcattat
     2341 ctatcaattt atagttgcga atgctttgtt tgattctaag catgatatta atcattatat
     2401 tcaatattta atacaactcg gctatccttt agacatgata actgacttgg attttgaaac
     2461 gttataccaa tatatgctaa gtgataaaaa gaatgataag caaggtgtac aaatggtctt
     2521 gattagacaa tttggagata tcgttgtaca acatgttgat caactaacat tacaacatgc
     2581 atgtgaacaa ttaaaaacat attttaagta ggtgaatgaa atggtaaatg aacaaatcat
     2641 tgatatttca ggtccgttaa agggcgaaat agaagtgccg ggcgataagt caatgacaca
     2701 ccgtgcaatc atgttggcgt cgctagctga aggtgtatct actatatata agccactact
     2761 tggcgaagat tgtcgtcgta cgatggacat tttccgactg ttaggtgtag aaatcaaaga
     2821 agatgatgaa aaattagttg tgacttcccc aggatatcaa tcttttaaca cgccacatca
     2881 agtattgtat acaggtaatt ctggtacgac aacacgatta ttggcaggtt tgttaagtgg
     2941 tttaggtatt gaaagtgttt tgtctggcga tgtttcaatt ggtaaaaggc caatggatcg
     3001 tgtcttgaga ccattgaaac ttatggatgc gaatattgaa ggtattgaag ataattatac
     3061 accattaatt attaagccat ctgtcataaa aggtataaat tatcaaatgg aagttgcaag
     3121 tgcacaagta aaaagtgcca ttttatttgc aagtttgttt tctaaggaac cgaccatcat
     3181 taaagaatta gatgtaagtc gaaatcatac tgagacgatg ttcaaacatt ttaatattcc
     3241 aattgaagca gaagggttat caattaatac aacccctgaa gcaattcgat acattaaacc
     3301 tgcagatttt catgttcctg gcgatatttc atctgcagcg ttctttattg ttgcagcact
     3361 tatcacacca ggaagtgatg taacaattca taatgttgga atcaatccaa cacgttcagg
     3421 tattattgat attgttgaaa aaatgggcgg taatatccaa cttttcaatc aaacaactgg
     3481 tgctgaacct actgcttcta ttcgtattca atacacacca atgcttcaac caataacaat
     3541 cgaaggagaa ttagttccaa aagcaattga tgaactgcct gtaatagcat tactttgtac
     3601 acaagcagtt ggcacgagta caattaaaga tgccgaggaa ttaaaagtaa aagaaacaaa
     3661 tagaattgat acaacggctg atatgttaaa cttgttaggg tttgaattac aaccaactaa
     3721 tgatggattg attattcatc cgtcagaatt taaaacaaat gcaacagttg atagtttaac
     3781 tgatcatcga ataggaatga tgcttgcagt tgcttctcta ctttcaagcg agcctgtcaa
     3841 aatcaaacaa tttgatgctg taaatgtatc atttccagga tttttaccaa aactaaagct
     3901 tttagaaaat gagggataat ataaaatgga agatatctat aaattaatag acgatatcaa
     3961 tctacaaaaa ctagaaaatt tagactctcg tgttaatgaa gcaataacta ctgacaacga
     4021 tgacgcatta tttattctag gagagacact ttacaatttt ggattaatgc cacaaggttt
     4081 ggaagtattc cgcgtgttat atcacaaata tccagacgaa agtgaattgc tgatttattt
     4141 tattgaaggt ttaatgtctg aaaatcaaac tgacgaagcg ttagaatatt taagttatgt
     4201 tgaaccatca cctgaaaagt tgatgttaga agcagattta tatcaacaaa ttaatatgat
     4261 ggaagttgct attgataaat tacaagaagc acttgaacta gagccaaatg atccaataat
     4321 ccattttgca ttggctgaaa tgttatatta tgatggtcaa tatttacgtg ctacctctga
     4381 atacgaaacc gttttagaaa ctggtgaata tcaagttaat ggtgtaaact tattctctcg
     4441 tatggcagat tgtagtttac aaagtggtaa ctatagtgat gcgattcgct tatacgatga
     4501 aattaatgaa gatgaaatga cttcagaaga ttatctcaaa aaagccattt cttacgataa
     4561 aaatgacatc actcaagaag caattaaaat aatgactaca ttactttcta aagatcctga
     4621 ttatattcaa ggctacttgt atttacaatc attatatgaa aatgaaaaaa attatccaga
     4681 tgccattgaa acgggtaaag aaggattacg actaagtcaa ttttataaag agttaatgta
     4741 tacaactgga tgtttggaaa tagaacacgg cgatgctaat gaaggtgtat tattgttaaa
     4801 acaagcatta gaggttgata atgcttacca agaaccttta ttgattttaa gcgatttata
     4861 tcgtaatgaa gaagattatg aagcaatcat tgaattatta acatatgtag atgaagaaga
     4921 tttggatcct acattcatgt ggcatttagc gttcgcctat ggtcaagaag agcgagataa
     4981 agaagctcaa catttctttg aattagcata tccaacgatg aaaacaaata ttgattttat
     5041 gagtgattat tatttctatt taattgaaat tggtcaaaag gaacaagcaa ttactatttt
     5101 aaatcaattg ttggaactag agccaagtaa cgaaaattgg catgacgaat tatcaagatt
     5161 gcaatcttaa aatggggtat ttgatgtcag agacattcaa tcaaattaaa gaaagtttta
     5221 ttgaatattt actttttcaa tatcgattta agtcgcgtat tgcagtttgg gtgcttaact
     5281 atataaaagt gaatgaggcg aaattggcaa acattcattt tgtagataca aaaattaatc
     5341 atcatgaaac attagaaatt gcagaagtgg gaagtcacgc ttctgcaatt caatttacta
     5401 agcgtaatat aaagttaatg aatacaaatg aaatatttga ttatatcgct aatcataact
     5461 gtgcttttga tattcaaatt cacttcgcaa acgtgtcaaa gcgtgagcaa aggctagatg
     5521 atttaatagt tgctcaacta acggaatcgc ccagttatca aacttatctt catgacttaa
     5581 atagtatggc tatagatcgg cataaacatg cgttattaat agattacttg ttacacaata
     5641 ttgatttaag tttacagatg aatgaaaagc aacgttttta tcaactaacg caaattttaa
     5701 atacactcaa gctagtaaat aaacataatc aatttgagga cttagcagat gacaattaaa
     5761 gcattttggc aatatacact ttatcaacgt tcttggttaa tgatgctact catttgtaat
     5821 atattaggga tgatttatgg ttatatatgg tatggtgaac aattaagtca tacgccctgg
     5881 cattttaaga tttttgttcc agatagccca actgcaatat tatacttagt tatttctatc
     5941 tcattgatat taattcaaaa acaaaatagt attatcgatg cactggcatt tgtaacatta
     6001 ttca
//
LOCUS       DANJFZ010000137         5906 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.138, whole genome shotgun sequence.
ACCESSION   DANJFZ010000137 DANJFZ010000000
VERSION     DANJFZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.138"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            63..770
                     /gene="splA"
                     /locus_tag="PWN45_002440"
     CDS             63..770
                     /gene="splA"
                     /locus_tag="PWN45_002440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDL0624179.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            895..1617
                     /gene="splB"
                     /locus_tag="PWN45_002441"
     CDS             895..1617
                     /gene="splB"
                     /locus_tag="PWN45_002441"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDL0624180.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1675..2394
                     /gene="splC"
                     /locus_tag="PWN45_002442"
     CDS             1675..2394
                     /gene="splC"
                     /locus_tag="PWN45_002442"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDL0624181.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2515..3234
                     /gene="splD"
                     /locus_tag="PWN45_002443"
     CDS             2515..3234
                     /gene="splD"
                     /locus_tag="PWN45_002443"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HDL0624182.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3183..3290)
                     /locus_tag="PWN45_002444"
     CDS             complement(3183..3290)
                     /locus_tag="PWN45_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624183.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3392..4108
                     /gene="splE"
                     /locus_tag="PWN45_002445"
     CDS             3392..4108
                     /gene="splE"
                     /locus_tag="PWN45_002445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HDL0624184.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4259..4978
                     /gene="splF"
                     /locus_tag="PWN45_002446"
     CDS             4259..4978
                     /gene="splF"
                     /locus_tag="PWN45_002446"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDL0624185.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5043..5177
                     /locus_tag="PWN45_002447"
     CDS             5043..5177
                     /locus_tag="PWN45_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624186.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5341..>5906
                     /locus_tag="PWN45_002448"
     CDS             5341..>5906
                     /locus_tag="PWN45_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL0624187.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2298 a          865 c         1048 g         1695 t
ORIGIN      
        1 tatatagtaa atgtgtatct agatacatta actataaaaa taaatttgga aggaggtaaa
       61 atatgaataa aaatgtaatg gttaaaggtt taactgcttt aactatttta acatctcttg
      121 gttttgctga aaatatttct aatcagcctc attcaattgc caaagcagaa aagaatgtca
      181 aagaaattac cgatgcaact aaggaaccat acaattcagt ggtagcattt gtgggtggta
      241 ctggtgtagt tgttggtaaa aatacaatcg taactaacaa acatatcgct aaaagtaatg
      301 atatttttaa aaatagagta tcagcacatc attcgagtaa aggtaaaggc ggaggaaact
      361 acgacgttaa agacattgta gaatatcccg gaaaagaaga ccttgcgata gttcatgttc
      421 atgaaacaag tacagaaggt ttgaatttta ataagaacgt tagttataca aaatttgcag
      481 acggagcaaa agtgaaagat agaatttctg ttattggtta tccaaagggt gcacaaacaa
      541 aatataaaat gtttgaatcg acaggaacga ttaaccatat cagtggaacg tttatggaat
      601 ttgatgcgta tgcacaacca ggtaattcag gatctcctgt attgaattct aaacatgaac
      661 tgattggtat tttatatgca ggtagtggaa aagatgaatc tgaaaagaat ttcggtgttt
      721 atttcacacc acaattaaaa gaatttattc aaaataatat tgaaaaataa gtatcatcaa
      781 ttcattcgtg aagttgattt tttaaagagt gattaagaaa acggttacat aattaactaa
      841 atatactgaa ttatgtatct agatatcaaa ataattaaaa gagaggaact taaaatgaac
      901 aaaaacgtag tcatcaagag tttagcagca ttaacaattt taacatctgt aacaggtatt
      961 ggaacaacat tggttgagga agtacaacaa actgccaaag cagaaaataa tgtcacaaaa
     1021 gttaaagata ctaatatttt tccatatact ggtgtagttg cttttaaaag tgcaactgga
     1081 tttgtagttg gaaagaatac tattttaaca aataaacatg tgtcgaaaaa ttacaaagtg
     1141 ggcgatcgta ttactgcaca tccaaatagt gataaaggta atggtggtat ttattcgatt
     1201 aaaaagatta ttaattatcc aggtaaagaa gatgtatcag tcattcaagt tgaagagcgt
     1261 gcaatagaac gtggaccaaa aggctttaat tttaatgata atgtaacgcc attcaaatat
     1321 gcggcagggg ctaaagctgg tgagcgaatt aaagtgattg gttatccaca cccatacaaa
     1381 aataaatatg ttttatatga gtcaactggc cctgtgatgt cagtagaagg tagcagtatt
     1441 gtatattcag cgcatactga aagcggaaac tctggatcac ctgtattaaa cagcaacaac
     1501 gaattagttg gtattcattt tgcttctgat gtaaaaaatg atgataacag aaatgcatat
     1561 ggcgtctact ttacaccaga aattaaaaaa ttcattgcag aaaacataga taaataaaca
     1621 aattgacttt aaacgagcgt tgcaacatat ctcgaattgt aaaggagctt gaaaatgaat
     1681 aaaaatatag tcattaaaag catggcagca ttagccattc taacctcagt aactggaata
     1741 aatgctgcag tcgttgaaga gacacaacaa atagcaaatg cagagaagaa tgttacgcaa
     1801 gttaaagata caaatatttt tccatataat ggcgtcgttt catttaaaga tgcgacaggt
     1861 tttgtaattg gaaaaaatac aattatcacc aataaacatg tatcaaaaga ttataaagtt
     1921 ggcgatagaa ttactgccca tccaaacggt gacaaaggaa atggtggtat atataaaatt
     1981 aaaagcattt ctgattatcc gggtgatgaa gacatctctg tcatgaatat tgaagaacaa
     2041 gcagtcgaac gtggaccaaa aggctttaat tttaatgaaa atgtccaagc attcaatttt
     2101 gcgaaagatg ctaaagttga tgacaaaatt aaagttattg gttacccatt acctgctcaa
     2161 aatagtttta aacagtttga atctacagga actataaaaa gaatcaaaga caatatttta
     2221 aattttgatg catacattga acccgggaat tcaggatcac cagttctaaa ttctaacaat
     2281 gaggtcatag gtgtggtgta tggcggtatt ggaaaaattg gttctgaata taatggtgcc
     2341 gtatacttta cgcctcaaat caaagatttt attcaaaagc acattgaaca ataaacaaat
     2401 ttaaatatac accatgagca tgtgttcaat aattttaatg aaaaacatcg gtcgaatata
     2461 acataaaaaa acgtctatat caaaagcatc atgaataaac agaggagcac aaaaatgaat
     2521 aaaaatataa tcatcaaaag tattgcggca ttgacgattt taacatcaat aactggtgtc
     2581 ggcacaacag tggttgatgg tattcaacaa acagccaaag cagaaaatag tgtgaaatta
     2641 attaccaaca cgaatgttgc accatacagt ggtgttacat ggatgggcgc tggaacagga
     2701 tttgtagttg ggaatcatac aatcattacc aataaacatg ttacttatca catgaaagtc
     2761 ggtgatgaaa tcaaagcaca tcctaatggt ttttataata acggtggtgg actttataaa
     2821 gttactaaga ttgtagatta tcctggtaaa gaagatattg cggtcgtaca agttgaagaa
     2881 aaatcaacgc aaccaaaagg tagaaaattc aaagatttca ctagcaaatt taatatagca
     2941 tcagaagcta aagaaaatga acctatatca gtcattggtt atccaaatcc taatggaaat
     3001 aaactacaaa tgtatgaatc aactggtaaa gtactatcag tgaatggaaa tatagtgaca
     3061 tctgatgcgg ttgtccaacc tggcagctct ggttcaccta tattaaatag taagcgagaa
     3121 gcaattggtg ttatgtatgc tagtgataaa ccaacaggtg aaagtacaag gtcatttgct
     3181 gtttatttct ctcctgaaat taagaaattt attgcagata atttagataa ataaatcatc
     3241 catccataca ttgataaatg atttttagaa attaacaaca aaatcaacaa ttttaaacat
     3301 ctctgtgatt ctatttattc gaaatgattt aaaaaataaa acttcaaaaa cctaacctta
     3361 tatttatacg aatacttaga ggagcacaaa aatgaataaa aatataatca tcaaaagtat
     3421 tgcagcattg acgattttaa catcagtgac tggcgtcggc acaacagtgg ttgagggtat
     3481 tcaacaaacg gctaaagctg aacataatgt gaaactaatc aaaaatacta atgtagcacc
     3541 atacaatggt gtcgtttcga taggatctgg aacaggtttc attgtcggta aaaatacaat
     3601 tgttaccaac aagcatgtcg ttgcaggtat ggaaattggt gcacatatta tagcgcatcc
     3661 caatggtgaa tataataatg gcggatttta taaagttaaa aaaattgtcc gttattcagg
     3721 tcaagaagat attgccattc tacatgtgga agataaagct gttcatccaa aaaacaggaa
     3781 ttttaaagat tacacaggca ttttaaaaat agcatcagaa gctaaagaaa atgaacgcat
     3841 ttcaattgtt ggctatccag aaccatatat aaataaattt caaatgtatg agtcaacagg
     3901 aaaagtgctg tcagttaaag gcaacatgat tattactgat gctttcgtag aaccaggcaa
     3961 ctcaggttca gctgtattta acagtaaata cgaagttgta ggtgttcact ttggtggaaa
     4021 cggccctgga aataaaagta caaaaggata tggtgtttat ttctctcctg aaattaagaa
     4081 attcattgca gataacacag ataaataaat ccttacatag ataaatgatt ttaaaaatta
     4141 acaacaaact caacaattca aatcatctct gtgattccat ttattcgaaa tgattaaaaa
     4201 aaataaaact tcaaaaagct aacattataa ttatacaaat acttagagga gcagaaaaat
     4261 gaataaaaat ataatcatca aaagtattgc agcattgacg attttaacat caataactgg
     4321 tgtcggcaca acaatggttg aaggtattca acaaacagcc aaagccgaaa atactgttaa
     4381 acaaattaca aatacaaatg ttgcaccata cagtggtgtt acatggatgg gcgctggaac
     4441 aggatttgta gttggaaatc atacaatcat taccaataaa catgttacct atcacatgaa
     4501 agtcggtgat gaaatcaaag cacatcctaa tggtttttat aataacggtg gtggacttta
     4561 taaagttact aagattgtag attatcctgg taaagaagat attgcggttg tacaagttga
     4621 agaaaaatca acacaaccaa aaggtagaaa attcaaagat ttcactagta aatttaatat
     4681 agcatcagaa gctaaagaaa atgaacctat atcagtcatt ggttatccaa atcctaatgg
     4741 aaataaacta caaatgtatg aatcaactgg taaagtatta tcagtgaatg ggaatatagt
     4801 gtcatcggat gcaattattc agcctggtag ctctggttca cctatattaa atagtaaaca
     4861 cgaagctatt ggtgtaatct atgccggtaa taagccatca ggtgaaagca caagaggatt
     4921 tgctgtttat ttctctcctg aaattaagaa attcattgca gataatttag ataaataatt
     4981 aaaacttaga cattcaccca atcctgacaa aatatactat aactaacatt tattaatata
     5041 tattgcatta tttaatatgc atcaaagcca atcaacgatt gattttcacc aactcaattg
     5101 ttgattggtt ttatttatgt atgaatgaac aactttttga catcattaag aatataaatg
     5161 attttgaaag catttgaaag ctacaacatt tctataaaat ttttcaataa caattgcgcc
     5221 actaaaactc aaaatttcca ccaccaacat ccaaattatc aacatcgcaa cataaccaaa
     5281 tgttataata aatctattac acaaagagat aaattactta tgcaaaggcg gaggaatcac
     5341 atgtctatta ctgaaaaaca acgtcagcaa caagctgaat tacataaaaa attatggtcg
     5401 attgcgaatg atttaagagg gaacatggat gcgagtgaat tccgtaatta cattttaggc
     5461 ttgattttct atcgcttctt atctgaaaaa gccgaacaag aatatgcaga tgccttgtca
     5521 ggtgaagaca tcacgtatca agaagcatgg gcagatgaag aatatcgtga agacttaaaa
     5581 gcagaattaa ttgatcaagt cggttacttc attgagccac aagatttatt cagtgcgatg
     5641 attcgtgaaa ttgaaacgca agatttcgat atagaacatc tagcgacggc gattcgcaaa
     5701 gttgaaacat ctacattagg tgaagaaagt gaaaatgact ttatcgggct gttcagcgat
     5761 atggatttga gttcaacgcg actaggtaac aatgtcaaag aacgtactgc tttaatctct
     5821 aaagtcatgg ttaatcttga cgacttacca ttcgttcaca gtgacatgga aattgatatg
     5881 ttaggtgatg catatgaatt cctaat
//
LOCUS       DANJFZ010000138         5888 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.139, whole genome shotgun sequence.
ACCESSION   DANJFZ010000138 DANJFZ010000000
VERSION     DANJFZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.139"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..460
                     /gene="tnpA"
                     /locus_tag="PWN45_002449"
     CDS             <1..460
                     /gene="tnpA"
                     /locus_tag="PWN45_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL0624188.1"
                     /translation="HTKWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQLCERKGVEI
                     IEAEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYGNRKFWCKGF
                     YVDTVGRNKKVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKKE"
     gene            complement(596..2188)
                     /gene="pckA"
                     /locus_tag="PWN45_002450"
     CDS             complement(596..2188)
                     /gene="pckA"
                     /locus_tag="PWN45_002450"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL0624189.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      2343..2453
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2559..3752
                     /locus_tag="PWN45_002451"
     CDS             2559..3752
                     /locus_tag="PWN45_002451"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL0624190.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            3877..4785
                     /locus_tag="PWN45_002452"
     CDS             3877..4785
                     /locus_tag="PWN45_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDL0624191.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            4997..5830
                     /locus_tag="PWN45_002453"
     CDS             4997..5830
                     /locus_tag="PWN45_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0624192.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         2029 a          882 c         1112 g         1865 t
ORIGIN      
        1 acatacaaaa tggaattgta agtatcatat tgtctttgca cctaaataca gaagacaagt
       61 gatatatgga aaaataaaaa aagatatagg gattatattg cgtcaattat gtgaaagaaa
      121 aggtgtagag ataattgaag cagaggcatg taaagatcat atccatatgt tagttagtat
      181 accacccaaa cttggggtat catcatttgt tggctattta aaaggaaaaa gtagtttaat
      241 gatatttgat agacatgcta acttaaagta tagatatgga aatagaaagt tttggtgtaa
      301 aggattttat gtggatacag taggtagaaa taaaaaagtg attgaaaatt atattcgtaa
      361 tcaattacaa gaggatatcg ttgcagacca aatctcaatg gaagaatatt tagatccttt
      421 cactggagag aaaaataaaa aaagaaagaa aaaagagtaa ccctttaggg ttgctggaat
      481 agtagtgcag ttggctgact tgtcagtgcc cttttagggc tggccagtaa ggaaggctta
      541 tagccgcaga acaaaccacc cgttcacacg ggtggttttg atttagtatt caaatttatt
      601 tgttgaagct acctttttca gcaatatgtt caactttttc accaaatttt tcgaagttct
      661 tttcaaaacg ttgaattaaa tcttctgctt gtgctttata tttctctttg tcgctccaag
      721 cattaattgg atttaaaatt gttttcggta catcttcaat ttctacagga atgcttaaac
      781 caaacgtact atcttttgta tattctgcat ttttcaattt gccagaaatc gcttggttta
      841 ccatttgacg tgtgtaatgt aagctgattc tacgtcctac accatatttt ccgccagtcc
      901 atccagtatt aacaagataa acatcaacat catgtaaatc gataagttca cctaatagat
      961 cagcgtaaac agtagggtgt aacgggaaga acggtgctcc gaaacatgtt gagaatgatg
     1021 gttcaggttc tgtcacacca cgctctgtac cagctaattt agaagtgaaa ccactcaaga
     1081 aatgatacat tgcttggtct ttatttaact ttgaaatcgg tggaataaca ccaaatgcat
     1141 ccgcagttaa gaaaataatt gtatttggat gtgctgcttt agatggtact acaatattgt
     1201 caatgtgatt aattggataa gcggcacgcg tgttttctgt ataacgattg tcttcaaagt
     1261 ccactgaacc atcttctgca actacagtgt tctctaaaat tgcaccatat ttgattgcgt
     1321 caaaaatctg tggttctttt tctttggaaa gattaattgc ttttgcatag cagccacctt
     1381 cgatattaaa gaccccgttt ttattccagc cgtgttcatc atcaccgatt agtttacggt
     1441 gtgggtcagc cgataaggtt gttttaccag tgccagatag accaaagaat aatgcaacat
     1501 cgcctttttc accaacgttt gctgagcaat gcatgctcat aatatcttgc atcggtaaga
     1561 gataattcat tacagagaag ataccttttt tcatttcacc agcgtattca gtaccgccga
     1621 ttaaaatgac tttgtgttta aatgaaataa tgacaaaggt ttcagattta gtaccatcaa
     1681 cttctggatc tgctttaaaa tgtggtgcag aaacgatagt gaagttaggt ttaatctttg
     1741 tagcttcttc ttttgattca ggtctaataa acatattttt agcaaataaa ttatgccatg
     1801 ctaattcatt gatgactgta agttttaaca ttgtatcttt atcgctacca gcgtagcctt
     1861 taaatacgta tagttcatct tttttatcta aatagtctag tactttatgg tataacttca
     1921 agaaagtttc ttcatcgata ggttgattaa tttctcccca atcaatgtta tctctatatg
     1981 aaggttcaga gacaaaaaat ttgtctttag gcgaacgacc agtatatttt ccagtacttg
     2041 cattaacagc accaagttct gttaataccc cttcgttatt gtctaagatt ttattataaa
     2101 gttgtgtcgt cgaaagttga aaatgtgacg ttggtttttt cagtaatttg tcaattttag
     2161 ttgtttcagt gtatgtgtct actgacatcc taaatccctc caaagcgata atttaaatgt
     2221 aagcctttac atttaattag tataacacat taacataatt aatctacaat attttaccgt
     2281 aaatttattt gtgaaaaatt gacattaatt cattaattag gtactattat ttttaacgga
     2341 ttctcttatc ctgagtggtg gagggacatg gacccaatga aacccagcaa cctctttttt
     2401 ataaaagaaa ggtgccaaac cgtttgcaga caaataggtc tgaacgataa gagcgaatgg
     2461 acgtattagg ccttctctct atattaatag ttagaaggtc ttttttattt agctcacaga
     2521 gagagaattt tcgtaatata aatttaaagg agcaaactat gttaaataac aaacgattat
     2581 ttacttcaga gtctgttaca gaaggacacc cagataaaat cgctgaccaa gtgtcagatg
     2641 caatattaga tgctatttta aaagacgatc caaatgcacg tgtagcttgt gaaacaactg
     2701 ttacaacagg tatggcattg atcgccggcg aaatttctac aacaacatat gttgatattc
     2761 cgaaagttgt tagagaaaca attaaagaaa ttggatacac aagagcaaaa tatggttatg
     2821 attatgaaac aatggcaatt ttaactgcga ttgatgaaca atcacctgac attgcacaag
     2881 gcgtggataa agcattagag tatcgtgata aagatagcga agaagaaatt gaagcaacag
     2941 gtgcaggtga ccaaggttta atgtttggtt acgcaacgaa tgaaacagag acgtatatgc
     3001 ctctagcaat ttatttatca catcaattgg caaaacgttt atcagatgtg cgtaaagatg
     3061 gtacgttaaa ttatttacga ccagacggta aagttcaggt cactgttgaa tatgatgaaa
     3121 atgataaccc agtacgtatt gatacgattg tcgtatcaac acaacatgct gaggatgtga
     3181 cacttgaaca aattcaagaa gacattaaag cacatgtcat ttatccaact gttccagaaa
     3241 acttgattaa tgaacaaact aaattctaca tcaatcctac aggacgcttt gtaatcggtg
     3301 gacctcaagg tgatgcagga ttaacaggac gtaaaatcat tgttgatacg tacggtggct
     3361 atgcacgtca tggtggcgga tgcttcagtg gtaaggatcc tacaaaagta gaccgttcag
     3421 ctgcatacgc tgcacgttat gtggctaaaa atattgttgc agcaggttta gcagatcaat
     3481 gtgaagtaca attggcatat gcgattggtg ttgcagaacc agtgtcaatt gcaattgata
     3541 catttggaac aggtaaagtt tctgaagggc aacttgtcga agcagttaga aaacactttg
     3601 acctaagacc tgcaggtatt attaaaatgc ttgatttgaa acaaccgatt tacaaacaaa
     3661 ctgctgctta tggtcatttt ggacgtacag atgtcttatt cccatgggaa aaattagaca
     3721 aagtggaaga attaaaagac gcagtaaaat aataagatga attgaatata gctaaataaa
     3781 agattttcgg tatataaatg ataatatgta tcgaaaatct ttagcttttc tattataatt
     3841 aaagttattc atatattaaa aaggagcgtt gttgtgatgg atttatcttc accgatagtc
     3901 attggactca ttattgcagt agtcattgca ttaatctttt ttgtcctttt tttagttgcc
     3961 ttaggtagta agaaaaaagt taagcgacaa acagaagaaa agtatgaaca acaagaacaa
     4021 aacattaaaa agtctcatga agaagcttta gagaaagagc gtattcaaaa taaaaagaca
     4081 attacaaaac aacaagaaga ttataatcac atggttagta caaaagatcg tgagattgac
     4141 gcattaaaat tgttctcgaa aaatcatagt gaatatgtaa cggacatgag attaattggt
     4201 attcgtgaaa gattagttaa agaaaaacga attcgaccag aagatatgca cattatggca
     4261 aatatctttt taccaaagga tggctttaat aatattgaac gaattagtca tttagtatta
     4321 acaagaactg gtctttatat tattgattct caattgttaa aaggtcatgt ttataatggt
     4381 attagtggcg gtcaatttaa agatttacca ccaatggaac aagtatttga tacattggat
     4441 ttagataaat ctagaccgca aactattgtg atggatcaaa atgatgacaa acgttcatta
     4501 tcatttgtaa attattcaga tcaaattgag gcgattaaac aattggctga agacttacaa
     4561 aaagaattag gtgccaaata tacaccaact tcaattttat actttaatct taaaaatgaa
     4621 ggcgatgtaa cgatttcaaa ttataatcaa aatagtgctg tcaaagtgct tgtcggtgct
     4681 gaacaattag acgagttctt taacaaattt gtattccatg gtcgtattca atacaacgtg
     4741 gaagacttac aacaaatgat ggataaaatt gaatcattca attaaatgtt aggctgaagt
     4801 gatgactata tgttgttact tcggcttttt tagtttgtca taaattaaac agaattactt
     4861 ttacaatttg attaatattt gagagatatt actagaattt tagtggttga ttgctctaag
     4921 tgttttttct catgtattca actcagattt gttatgataa cagggtataa tacatcaaaa
     4981 aggtgaggcg atatttatgg aggttaaaac attttataat ggaaacacga tgccacaaat
     5041 tggtttaggg acatttcgtg tggaaaatga tgaaaattgc atggaaagtg ttaagtatgc
     5101 cattgaacaa ggatatcgta gtattgatac cgcaaaagtt tatgggaatg aagaacaagt
     5161 aggtgctggc attcgtgccg gattggagtc aactggtatt gctagagaag acttatttat
     5221 tacttcaaaa ttatattttg aggatttcgg tcgtgaaaat gtagcagctg cttacgaagc
     5281 tagtttatct agattaggtt taaaatactt agatttgtat ctagtacatt ggccaggtac
     5341 gaacgaagcc gtaatggttg atacatggaa aggtatggaa gatttatata aaaataataa
     5401 tgttaaaaat ataggtgtca gcaattttga acctgaacat ttggaagctt tgttggcaca
     5461 agtgtcaatt aaacctgtaa ttaatcaagt tgaatatcat ccatatttaa cccaacataa
     5521 attgaaatta tatttggcag cacaacgtat cgtgatggaa tcttggtcac cattgatgaa
     5581 tgcacaaatt ttaaatgatg agacaattaa agacattgct caagaattag gaaagtcacc
     5641 tgcccaagtt gttttaagat ggaatgtgca gcatgctgtg gttacaatcc ctaaatcggt
     5701 gacaccaaac agaatctctg aaaatttcca aatatttgat ttcgaattat cagatgaaca
     5761 aatgacgcga attgatggtt taaatcaaga taagagaatt ggacctgatc caaaaaaatt
     5821 tgaaggctag attaaaatcg ctcaactgat gaaaaggtta gatgaattgt cagggcttgg
     5881 gacattaa
//
LOCUS       DANJFZ010000139         5821 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.140, whole genome shotgun sequence.
ACCESSION   DANJFZ010000139 DANJFZ010000000
VERSION     DANJFZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.140"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            50..1879
                     /locus_tag="PWN45_002454"
     CDS             50..1879
                     /locus_tag="PWN45_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0624193.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(2019..4097)
                     /locus_tag="PWN45_002455"
     CDS             complement(2019..4097)
                     /locus_tag="PWN45_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL0624194.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(4235..5644)
                     /locus_tag="PWN45_002456"
     CDS             complement(4235..5644)
                     /locus_tag="PWN45_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0624195.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT         1967 a          907 c         1021 g         1926 t
ORIGIN      
        1 taggcgtata gtgatatcca tcaaaaacta taaaaaggaa taggagatta tgttcaatca
       61 atttaaaaga cttattatag ggcaacctaa aaaaaacaga gaactaaaag acgaaaagat
      121 atcgaagttt aaagggttag cgattctttc gtcggatgca ttatcttcag tggcttacgg
      181 tcccgaacaa atactgatta cactatcagt agtgggtgca gttgcaactt ggtatacttt
      241 accgattgca ggtgctgttc tgatcttatt agctgcttta attatgtctt ataggcaaat
      301 tatttatgca tatcctaaag gtggcggcgc gtatatggtg tccaagacca atttaggaga
      361 aaaatgggga ttgcttgcag gaggctcatt attagtcgat tacatactga ctgtcgctgt
      421 tagtatatca tctggtgctg atgcatttgt tgctgcattc ccaagtttat atgggcataa
      481 agtactgatt gcatgtttac ttgtactttt tattttaata ttaaatttac gtggtttaac
      541 tgaatctgcc accgtattat cctatccagt atatcttttc attatagggt tagtgatatt
      601 aatatttata ggtactttcc gtgtggcgac aggagatatt caaccacata tgcatgcatc
      661 agtaggaact gcggttcctg gagtaacatt attcttgcta ttaaaagcat tttcatcagg
      721 ggcttcatcg ttaacgggtg ttgaagctat ttcaaatgcg gtaaccaatt ttagagagcc
      781 aagtgctaaa aatgctgtga aaacgttaat tgctatgggt tcaattttag cgtttttatt
      841 agttggtatc gttggtttag catatgttta tggaattttg ccacaaacgg aaacgacagt
      901 tttatcacaa ttagctatgc aaatatttgg tgataatgct gccttctatt ttgtacaggc
      961 aacgaccgtg atgattttgg tattagcagc caacactgga tttacagctt ttccgatgtt
     1021 agcggcttca atgtctaaag ataaatatat gcctagaatg tttactgtta gaggtgatcg
     1081 cttaggctac tctaactcga ttattatttt aggtgtatta gccattattt taattattgt
     1141 atttgatggt atgactgaag atttaattcc attgtatgcg gttggtgtat ttattccatt
     1201 tacactagca caattcggta tggttattaa atggattcat gaacgtccta agaattggtt
     1261 aagtaagttg tctgtgaact tacttggtgg tatcgtgaca tttattgttt ttatgatttt
     1321 actcataact aaatttagcc aagtatggcc gatacttatt ttcttgccgt tcgtggtgat
     1381 tttcttcttg aaaattaaca aacattatcg agatatcgca gaacaattac gttctgacat
     1441 tgatgttctt aatgtggacg tggtagatcg taatttagca attgtgccga taacaagtat
     1501 tacaacagca gttgataagt ctatttatta cgcacaaatg cttgcgaata atgatgtcat
     1561 agcagtacat gtatcatttg gagatgaaga tgaaaaagca ttccaagaga aatggaaacg
     1621 tcatttccca gatgtaagat tagttatttt acattcggaa tatcgcagta ttatacgacc
     1681 aatttcacgt tttatcgata agattaatcg taaagccaac gatcaaaact atatgattac
     1741 agtagtgata ccagaattta ttactaagaa acgttggcat aatttattac acaatcaaac
     1801 tagtttgcgt atgaaattgt atttaattta tcaaaagaat gttaacgtat gtacaattcc
     1861 atttaaactt aaaaaataga ttctataatc aaatcggatt gatgaaaggt tatttacttt
     1921 tcatcaatct gattttttga tgttgaatat aaaaaccccg tacatatgag atgataatga
     1981 ctttgtctca tatgtacggg ggtattgtta tgtcttgctt aatgtttgga ctgtaaagac
     2041 aagacaattt cgttgtaatt gatattttct cgaatttgag ctgcaacgat attgttgatt
     2101 ttatttttgc gaattaattc atctaaaaga tgacgttgta agtacaatgc ttccaatttg
     2161 tggttatgga acatagcttg cggtatgatt tgattgctgt tttcttgacg ttcttttcga
     2221 cgttgtgcac gccactttaa tgagcgactc atatcatagt aatgattaat gactaagcta
     2281 acctcaagta catttgtact attttgttct tcttttaagc gtaaaataat attgtgattc
     2341 acaatacgca taattttttg aacatctttg aatgaatttt caaattcttg cttcttagat
     2401 tctttattta aggctttgta gtgcttcatc gctttaagtc taagccataa catcttgaag
     2461 aagtttgcaa atttttcaac aaatgatgca gtacggtgtg tctctgttaa ttcgacaata
     2521 ttgcggtaat tattaatatc ctgatatgtt gccttacctt tatcaattaa acgctcaagt
     2581 gtagatgttt cgattacttt tgcaatatct tctaaacgtt tgagttcttt tgtattttga
     2641 ttatcaggtt ctgaatttaa taaaaataac aattctccat agtattggtt taatactggg
     2701 cgataatttg tatcgttttt gtctttttta ctttcgtttt taaaatgctg gatcactttt
     2761 tgaacaatga aaattttggc agattgataa gtcatacctt taaaagtagt atcttcggca
     2821 gatggtgtaa ttaaaggtaa aacaatttgc gctaagatta aactaattaa taccatgaaa
     2881 gatgcaataa acaataaatc attacggtat tcgaatgctt gtccttttgt aatgataaat
     2941 ggtaatgtaa gtgccattga aagtgaaatt gtaccgtgaa taccacacat ggtcataata
     3001 aatgcgtaac gcactcgaga aggtggtgtt tcatgtgaat catgttcctc gtctagataa
     3061 gattgtatat ttttcgggaa ataaaaatct ttgtaccaga aataaaccca aacaaatcga
     3121 catgcataaa ttgcaatagc gattaatata gtgatcacta ttaaaaatga aaagttgtca
     3181 ggctctgttt ggaaaatatc tatgactaca gtaggaatca taaaacctaa tacaacaaat
     3241 acaaaaccat ttaaggcata actaaatgtg ttccaaattt gatggtaatt catttgtagt
     3301 tccgtttgtg ctctaattaa acggtcgcgt tctaaaccat ggattagacc tgcgattaca
     3361 actgcaatga tacctgaagc atgaacttct tctgctaaaa agtatacgac aaaaggagtt
     3421 aataattgaa taaaagttaa ggtattgtta tcttttaaac ctttattagc ggttaagtct
     3481 atacgtattc taacgacaac gaatccaata attgcaccaa taagtacacc tagtattgtt
     3541 gaaatgataa attgttcaac agcttggaat aatgaaaagg taccagttac taatgcagta
     3601 acagcaattt taaatgaaat gatacctgct gcatcattga gtaaagattc accttctaaa
     3661 atcgtcatag aacctttagg taataatttt ccgcgtgtaa tagcagatac tgctactgca
     3721 tcagtaggac ataaaattgc tgctattgca aaagcggctg gcattggtaa ggcaggccaa
     3781 atccaatgta taaaatagcc aacaccgact acagttgcaa acactagtgc cattgacatt
     3841 aatagtatag gtttacgata ttctaataat tttgttcgag agacgtgggt accttccaca
     3901 aaaagtagtg gcgcgataac ggcaaacata aatacttcag aattgaattg gaaatcaact
     3961 tgtattggaa taatgaaaat aacgacacct aatgcaattt gaataaaggc agtaggaatt
     4021 tgtgggaatc gattattgat aaccgaacta ataatcacag caaaaataaa aattaaaaat
     4081 gcttctaata gtgccataca atactcctca aaattttaat agttaatatt ttatcacttt
     4141 taaggcataa tgacatagat atattgataa aatgaagtta ttttcaaaaa aactctagta
     4201 tcggttgaac tgatactaga gcgagatgtt taaattattg attgtcatat ctgaaatgac
     4261 cgctgtcatt ttgtcgttgt tcatacgcga gcttttcagc attcgttttg tattttttat
     4321 aaaagaaaaa taaaaatata aaccagaatg gcgaaatata aatagctgct cttgtttcgt
     4381 cactaaagaa taataaaatg aatacaaaga agaagaacgc tagaataatg taagcaatag
     4441 gcttaccacc aatcaactta aatttactgt ttttatgtgc ctcaggatgc tttttcaaat
     4501 acattagata agcgacaata atcattgccc aaacaactaa aaacaacaca gttgataacg
     4561 ttgtaacgta tatgaatagt tgaattgcat tagggaaaat gtagtttaac aaggctgcta
     4621 taagtaatgc aattgatgaa actaacattg ataaataagg cacgccatga ctattcgttt
     4681 tatttaaaac tttaggacct aacccttgtt gtgacagtcc gaataagata cgactattcg
     4741 aatagatacc actatttgta gcagaggccg cggcagttag cacgacaaag ttaacgacac
     4801 ctgctgcaaa tggtacgccg attaatgtaa agagtttaac gaaagggcta ctatttggat
     4861 cgatatcatt ccaaggtatg actgacataa ttactaatag accaccgata tagaataata
     4921 aaatacggat aggtacatta ttaattgctt tcggtaaggt tttttcagga tctttcgttt
     4981 caccagcagt tacacctata agttcaatac caatgaatga atatacagca atttggaatg
     5041 acattaagaa accaaatgtt ccaccaggga acatgccacc gtgactgatt aagtttgtga
     5101 atgatgcatg tccataatgt gttttaaatg agaaaaagat taatacaaga ccaacgataa
     5161 ccatcgcaat aattgtgaca actttaataa tcgagaacca aaactccagt tcaccaaata
     5221 atctggcacc taataagttg aagctaatca agattaaaac aataaatagc acggtaatcc
     5281 aatttgggac ttgtggatac caaaatgcaa agtattgtcc catcgcagtt aggtctgaca
     5341 tacttgatac aatccaacat aaccagtaag tccagccaat gacaaagcca ccaaaagggc
     5401 ctaaatattc atttgcaata tcaacaaatg aattaaatct tgtattgctc aacaacaatt
     5461 cgcctaatgc gcgcataaaa gcgaatagta caaccccaat aatcatgtat gtgaataaca
     5521 gtgatggacc agttaaagat attgtttgac ctgatcctag gaataaccca gttccaattg
     5581 cgcccccaat tgctattaat tggatgtgtc tgttattcaa ctctctatgc aattttcttg
     5641 ccataatttt cctcctcagg ataaagcaaa taaaaaagtt gtgtgtaagc gttttcttaa
     5701 ttataaatac taaagaaatc catttaataa agcaattatt tttttaaata taagaaaaaa
     5761 catttgatag gtaagggtat gataactaaa acttatcgat tttttgaaaa gatataagtt
     5821 t
//
LOCUS       DANJFZ010000140         5784 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.141, whole genome shotgun sequence.
ACCESSION   DANJFZ010000140 DANJFZ010000000
VERSION     DANJFZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.141"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            25..1005
                     /locus_tag="PWN45_002457"
     CDS             25..1005
                     /locus_tag="PWN45_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL0624196.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            968..1660
                     /locus_tag="PWN45_002458"
     CDS             968..1660
                     /locus_tag="PWN45_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0624197.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1870..3036
                     /locus_tag="PWN45_002459"
     CDS             1870..3036
                     /locus_tag="PWN45_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0624198.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3017..4255
                     /locus_tag="PWN45_002460"
     CDS             3017..4255
                     /locus_tag="PWN45_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL0624199.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4245..5675
                     /locus_tag="PWN45_002461"
     CDS             4245..5675
                     /locus_tag="PWN45_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDL0624200.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         1885 a          790 c         1050 g         2059 t
ORIGIN      
        1 atatattttt aatgtataaa tgaggtggaa gatttggaaa gagttttgat aactggtggg
       61 gctggtttta ttgggtcgca tttagtagat gatttacaac aagattatga tgtttatgtt
      121 ctagataact atagaacagg taaacgagaa aatattaaaa gtttggctga cgatcatgtg
      181 tttgaattag atattcgtga atatgatgca gttgaacaaa tcatgaagac atatcaattt
      241 gattatgtta ttcatttagc agcattagtt agtgttgctg agtcggttga gaaacctatc
      301 ttatctcaag aaataaacgt cgtagcaaca ttaagattgt tagaaatcat taaaaaatat
      361 aataatcata taaaacgttt tatctttgct tcgtcagcag ctgtttatgg tgatcttcct
      421 gatttgccta aaagtgatca atcattaatc ttaccattat caccatatgc aatagataaa
      481 tattacggcg aacggacgac attaaattat tgttcgttat ataacatacc aacagcggtt
      541 gttaaatttt ttaatgtatt tgggccaaga caggatccta agtcacaata ttcaggtgtg
      601 atttcaaaga tgttcgattc atttgagcat aacaagccat ttacattttt tggtgacgga
      661 ctgcaaacta gagattttgt atatgtatat gatgttgttc aatctgtacg cttaattatg
      721 gaacacaaag atgcaattgg acacggttat aacattggta caggcacttt tactaattta
      781 ttagaggttt atcgtattat tggtgaatta tatggaaaat cagtcgagca tgaatttaaa
      841 gaagcacgaa aaggagatat taagcattct tatgcagata tttctaactt aaaggcatta
      901 ggatttgttc ctaaatatac agtagaaaca ggtttaaagg attactttaa ttttgaggta
      961 gataatattg aagaagttac agctaaagaa gtggaaatgt cgtgaaaatg acattgaagc
     1021 tgtccataat aataagggtt atgcctatca aagaaaatta gacaaactag aagaagtgag
     1081 aaaaagctat tacccaatta aacgtgcgat tgacttaatt ttaagcattg ttttattatt
     1141 tttaacttta ccgattatgg ttatattcgc cattgctatc gtcatagatt cgccaggaaa
     1201 ccctatttat agtcaggtta gagttgggaa gatgggtaaa ttaattaaaa tatacaaatt
     1261 acgttcgatg tgcaaaaacg cagagaaaaa cggtgcgcaa tgggctgata aagatgatga
     1321 tcgtataaca aatgtcggga agtttattcg taaaacacgc attgatgaat taccacaact
     1381 aattaatgtt gttaaagggg aaatgagttt tattggacca cgcccggaac gtccggaatt
     1441 tgtagaatta tttagttcag aagtgatagg tttcgagcaa agatgtcttg ttacaccagg
     1501 gttaacagga cttgcgcaaa ttcaaggtgg atatgactta acaccgcaac aaaaactgaa
     1561 atatgacatg aaatatatac ataaaggtag tttaatgatg gaactatata tatcaattag
     1621 aacattgatg gttgttatta caggggaagg ctcaaggtag tcttaattta cttaataagt
     1681 tcaaataaaa gttatatttt aaagattgtg accaattgtt acagtataac gaggaatccc
     1741 ttgagacagt atcaaatggc attaagaaat atgtgccatc attgatttgc atggctataa
     1801 atactattca tctgatgaga tagccatgtt aagaaattga aagtatagca ttaaaggggt
     1861 ttgtaacagt tgaaaattat atattgtatt actaaagcag acaatggtgg tgcacaaaca
     1921 catctcattc aactcgccaa ccatttttgc gtacacaatg atgtttatgt cattgtaggc
     1981 aatcatggac caatgattga acaactagat gcaagagtta atgtaattat tatcgaacat
     2041 ttagtaggtc caattgactt taaacaagat attttagctg tcaaagtgtt agcacagtta
     2101 ttctcgaaaa ttaaacctga tgttatccat ttacattctt ccaaagctgg aacggtcgga
     2161 cgaattgcga agttcatttc gaaatcgaaa gacacacgta tagtttttac tgcacatgga
     2221 tgggctttta cagagggtgt taaaccagct aaaaaatttc tatatttagt tatcgaaaaa
     2281 ttaatgtcac ttattacaga tagcattatt tgtgtttcag atttcgataa acagttagcg
     2341 ttaaaatatc gatttaatcg attgaaatta accacaatac ataatggtat tgcagatgtt
     2401 cccgctgtta agcaaacgct aaaaagccaa tcacataaca atattggcga agtagttgga
     2461 atgttgccta ataaacaaga tttacagatt aatgccccga caaagcatca atttgttatg
     2521 attgcaagat ttgcttatcc aaaattgcca caaaatctaa tcgcggcaat agagatattg
     2581 aaattacata acagtaatca tgcgcatttt acatttatag gcgatggacc tacattaaat
     2641 gattgtcagc aacaagttgt acaagctggg ttagaaaatg aagtcacatt tttgggcaat
     2701 gtcattaatg cgagtcattt attatcacaa tacgatacgt ttattttaat aagtaagcat
     2761 gaaggtttgc caattagcat tatagaagct atggctacag gtttgcctgt tatagccagt
     2821 catgttggcg gtatttcaga attagtagct gataatggta tatgtatgat gaacaaccaa
     2881 cccgaaacta ttgctaaagt cctggaaaaa tatttaatag acagtgatta catcaaaatg
     2941 agtaatcaat ctagaaaacg ttatttagaa tgttttactg aggagaaaat gattaaagaa
     3001 gtggaagacg tttataatgg aaaatcaaca caatagtaaa ttactaacat tgttacttat
     3061 cggtttagcg gtttttattc agcaatcttc ggttattgcc ggtgtgaatg tttctatagc
     3121 tgactttatc acattactaa tattagttta tttactgttt ttcgctaacc atttattaaa
     3181 ggcaaatcat tttttacagt ttttcattat tttgtataca tatcgtatga ttattacgct
     3241 ttgtttgcta ttttttgatg atttgatatt tattacggtt aaggaagttc ttgcatctac
     3301 agttaaatat gcatttgtag tcatttattt ctatttaggg atgatcatct ttaagttagg
     3361 taatagcaaa aaagtgatcg ttacctctta tattataagc agtgtgacta taggtctatt
     3421 ttgtattata gctggtttga acaagtcccc tttactaatg aaattgttat attttgatga
     3481 aatacgttca aaaggattaa tgaatgaccc taactatttc gcgatgacac agattattac
     3541 attggtactt gcttacaagt atattcataa ttacatattc aaggtccttg catgtggtat
     3601 tttgctatgg tctttaacta caacggggtc taagactgcg tttatcatat taatcgtctt
     3661 agccatttat ttctttatta aaaagttatt tagtagaaat gcggtaagtg ttgtgagtat
     3721 gtcagtgatt atgctgatat tactttgttt taccttttat aatatcaact actatttatt
     3781 ccaattaagc gaccttgatg ccttaccgtc attagatcga atggcgtcta tttttgaaga
     3841 gggctttgca tcattaaatg atagtgggtc tgagcgaagt gttgtatgga taaatgccat
     3901 ttcagtaatt aaatatacac taggttttgg tgtcggatta gtggattatg tacatattgg
     3961 ctcgcaaatt aatggtattt tacttgttgc ccataataca tatttgcaga tctttgcgga
     4021 atggggcatt ttattcggtg cattatttat catatttatg ctttatttac tgtttgaatt
     4081 atttagattt aacatttctg ggaaaaatgt aacagcaatt gttgtaatgt tgacgatgct
     4141 gatttacttt ttaacagtat catttaataa ctcaagatat gtcgctttta ttttaggaat
     4201 tatcgtcttt attgttcaat atgaaaagat ggaaagggat cgtaatgaag agtgattcac
     4261 taaaagaaaa tattatttat caagggctat accaattgat tagaacgatg acaccactga
     4321 ttacaatacc cattatttca cgtgcatttg gtcccagtgg tgtgggtatt gtttcatttt
     4381 ctttcaatat cgtgcaatac tttttgatga ttgcaagtgt tggcgttcag ttatatttta
     4441 atagagttat cgcgaagtcc gttaacgaca aacggcaatt gtcacagcag ttttgggata
     4501 tctttgtcag taaattattt ttagcgttaa cagtttttgc gatgtatatg gtcgtaatta
     4561 ctatatttat tgatgattac tatcttattt tcctactaca aggaatctat attataggtg
     4621 cagcactcga tatttcatgg ttttatgctg gaactgaaaa gtttaaaatt cctagcctca
     4681 gtaatattgt tgcgtctggt attgtattaa gtgtagttgt tatttttgtc aaagatcaat
     4741 cagatttatc attgtatgta tttactattg ctattgtgac ggtattaaac caattacctt
     4801 tgtttatcta tttaaaacga tacattagct ttgtttcggt taattggata cacgtctggc
     4861 aattgtttcg ttcgtcatta gcatacttat taccaaatgg acagctcaac ttatatacta
     4921 gtatttcttg cgttgttctt ggtttagtag gtacatacca acaagttggt atcttttcta
     4981 acgcatttaa tattttaacg gtcgcaatca taatgattaa tacatttgat cttgtaatga
     5041 ttccgcgtat taccaaaatg tctatccagc aatcacatag tttaactaaa acgttagcta
     5101 ataatatgaa tattcaattg atattaacaa tacctatggt ctttggttta attgcaatta
     5161 tgccatcatt ttatttatgg ttctttggtg aggaattcgc atcaactgtc ccattgatga
     5221 ccattttagc gatacttgta ttaatcattc ctttaaatat gttgataagc aggcaatatt
     5281 tattaatagt gaataaaata agattatata atgcgtcaat tactattggt gcagtgataa
     5341 acctagtatt atgtattatt ttgatatatt tttatggaat ttacggtgct gctattgcgc
     5401 gtttaattac agagtttttc ttgctcattt ggcgatttat tgatattact aaaatcaatg
     5461 tgaagttgaa tattgtaagt acgattcaat gtgtcattgc tgctgttatg atgtttattg
     5521 tgcttggtgt ggtcaatcat tatttgcccc ctacaatgta cgctacgctg ctattaattg
     5581 cgattggtat agtagtttat cttttattaa tgatgactat gaaaaatcaa tacgtatggc
     5641 aaatattgag gcatcttcga cataaaacaa tttaagtacc ggtaatgcta tactttagaa
     5701 aattaagatt aagaagaaaa ggcaatttct tattgaaaaa tggaagttgt cttttttaat
     5761 tctctttaaa agcgggaaac aaaa
//
LOCUS       DANJFZ010000141         5752 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.142, whole genome shotgun sequence.
ACCESSION   DANJFZ010000141 DANJFZ010000000
VERSION     DANJFZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.142"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..350
                     /gene="fmtA"
                     /locus_tag="PWN45_002462"
     CDS             <1..350
                     /gene="fmtA"
                     /locus_tag="PWN45_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDL0624201.1"
                     /translation="YGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYP
                     DEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILK
                     QNKPYNTKGVIVQ"
     gene            complement(887..1165)
                     /gene="qoxD"
                     /locus_tag="PWN45_002463"
     CDS             complement(887..1165)
                     /gene="qoxD"
                     /locus_tag="PWN45_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDL0624202.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(1174..1779)
                     /gene="qoxC"
                     /locus_tag="PWN45_002464"
     CDS             complement(1174..1779)
                     /gene="qoxC"
                     /locus_tag="PWN45_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDL0624203.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(1769..3757)
                     /gene="qoxB"
                     /locus_tag="PWN45_002465"
     CDS             complement(1769..3757)
                     /gene="qoxB"
                     /locus_tag="PWN45_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDL0624204.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(3757..4857)
                     /gene="qoxA"
                     /locus_tag="PWN45_002466"
     CDS             complement(3757..4857)
                     /gene="qoxA"
                     /locus_tag="PWN45_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDL0624205.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            5159..5281
                     /locus_tag="PWN45_002467"
     CDS             5159..5281
                     /locus_tag="PWN45_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624206.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(5429..5746)
                     /locus_tag="PWN45_002468"
     CDS             complement(5429..5746)
                     /locus_tag="PWN45_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDL0624207.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         1995 a          929 c         1002 g         1826 t
ORIGIN      
        1 actatggtgc aggtaattta tatatgacac caacagatat gggtaaatta attactcaaa
       61 tacaacaata taaattattc agtcctaaaa taaccaatcc attattacat gagtttggta
      121 cgaaaaagta tccagatgaa tatcgatatg gtttctatgc taagccaaca ttgaatagac
      181 ttaacggggg attctttgga caagtcttta ctgtttacta taatgataag tatgtagttg
      241 tacttgcatt aaatgtaaaa ggaaacaatg aagttcgaat caaacatatt tataatgata
      301 ttttaaaaca aaataaacct tacaatacga agggtgttat tgttcaataa ttaatataga
      361 agatataaca tgtatatggc attaaggcat cgaccttatc tgaccagtat acgagttata
      421 tcttcttttt tatagtggta aaaagtttaa agtataaggt tgaagaagga tgagtttaaa
      481 aatatgtgtt aactgataaa aggggaaatc atttggtgag ttggcatcag actaaaatga
      541 atgaagacga atacgttggt ccatgcgtgg tgtgaatgtt ttctaaatct aactggaaat
      601 gataatttaa taatgtacac ttttgattgt ttaaacatgt acaattttta aaaacctagg
      661 gataatttta ttgacgtgaa caacattgag attatcttta gacataaata tggattttga
      721 agttactata tttattagtt gtggttgtta atgtattaat tgaattgatt ataatgattg
      781 tcttataaga tatagaaggg gacttaacca tttaaaaatt gaaggaaaat aaaaaccagc
      841 taacaaaagt gttagctggt taaactttac attattaaaa tgttgtttat aagtgtgaag
      901 agtgaccgcc ttgcataacc caatatgttc cgacaacgaa acaaagtgta attacaagag
      961 caaagataac tttgaatgtt tgtaaacgtc catctttacc ttcagttaaa tgcatgaaca
     1021 ttaataattg aagtcctgct tggacgaatg caaagccaaa gataattgtc aacttcgcgt
     1081 ggaatgttaa tgacgtgtat agtgttacgt aaactgctaa aagcgttaat acgatagatg
     1141 cgataaatcc tacagtatgt ttcattattg tactcatccg ctatacacca tccctatcat
     1201 atatacggca gtaaagatga aaacccaaac aacatctaag aagtgccagt ataaacttac
     1261 tataaataat tttggcgcat tatatttgtc taatccgcgt cgttggattt ggattaataa
     1321 acaaatggcc caaacgatac ctagcgatac gtgacaacca tgcgttccta ataggataaa
     1381 gaaactagac cagtaagaac caattgttgg gttaacgcct tctgatgcat agtgtgcgaa
     1441 ttcataaatt tcgaatccaa caaagactaa acctaaaagt aacgtaatga tcatccaaaa
     1501 catcattaac ttttgttttt cttggcgcat gtagtaaata gcaataccac atgtgtaaga
     1561 actgaataat aatgcaaacg tcattattaa aacaagaggc aattcaaata actcagtagt
     1621 cattttacct gcataatcgc caccatgttg caaagttaat agtgtcgcaa atagggtacc
     1681 gaataacgca aattcggctg taatgaaaat ccaaaagcca agcttattta attcgccttc
     1741 atgtgtgcgt gaatcaatag tgtttgtatc atgactcatg acttacagcc tccctttctt
     1801 taatacgcgc ttctcttaat ctagcttcag tttctgcaac ttcagcagca gggatatgat
     1861 atccgtgatc gatttggaaa ctgcgataaa tcatagtacc aaaaataccg aataaacaaa
     1921 ttaatgctgg aattacagtt tcgaaaatta agaagaaacc gccgatagtc ataaagatac
     1981 caatccagaa tccaacagga gtattgtttg gcatatgaat gtctttgtaa ttatggttgt
     2041 ctaagtaatg acgaccatgt tctttcatat caacaaatgt gtcgtagtca ttccaatctg
     2101 gtgtaatggc aaagttgtat ttaggtggaa ttgctgatgc agtagtccac tctagagtac
     2161 gaccaaggcc atcccagtta tctccagttg cttcacgtgg agatttgaag tgactgtata
     2221 cgatactaac aacaaggaat aagaatccga ttgccattaa taatgcaccg atagttgaaa
     2281 ttaagtttaa taagaaccaa ccatctgatg gcatataagt gtataaacga cgtggcatac
     2341 catctaatcc aagaatgaat tgtggtaaga aacaaacgtt aaatccgatc atgaagaacc
     2401 agaagcacca tttgtttaat gtttcgttta atttgtaacc catcatcttt ggataccaga
     2461 agattaaacc agctaagcag gcaaatacaa caccagtaac caatgtatag tggaagtgag
     2521 ctactaagaa gtacgtattg tgatattgat agtcagctga tgccattgct aacattacac
     2581 ccgtaacacc acctaaaagg aagttaggga taaatgctaa tgagaatagc attggtgact
     2641 caaatgtaat acgtccttta tataatgtta ataaccagtt aaacaatttc acaccagttg
     2701 gaataccaat cagcattgtt gaaattgaga agaatgagtt gattaacgca ccattaccca
     2761 ttgtgaagaa atggtgaacc caaactaaga aactaaggaa cgcgatgccg gcagttgccc
     2821 ataccatact ttgatgtccg aataaacgct tacgagcgaa tgtcgggata atttctgagt
     2881 aaataccaaa tgctggaagg ataacgatat aaacttcagg gtgcccccat acccagaaga
     2941 agttagccca aagcattggc ataccgccat gtgcaactgt gaagaatgct gtgtcaaata
     3001 ttctatcagt tgtcattaat gctaacgcta ctgttaaagg agggaaagca agaataacaa
     3061 ttaatgtagt aataaatgtt gttactgtaa acattggcat ttgcataaac ttcatagttg
     3121 gtgttttaca tcttaaaatt gttacaaaga agttgatacc tgtagctaag gtaccaagcc
     3181 ctgaaatttg tatagctatt aagtaatagt taacacccgg accaggactg aattcacctg
     3241 ctagtggcgc atagtttgtc caaccagctg ctggtgaacc accaataata aatgacaggt
     3301 tgaataaaat catacctgca aagaatagcc agaaacttac gttgtttaat actgggaatg
     3361 caacatcacg tgctccaatt tgtaatggaa caacgatatt ccataaacca aagataaatg
     3421 gcattgccat gaagataatc atgattacac catgtgtact aaaaatttcg ttatagtggt
     3481 tagattctaa aaatttgtta tcaggtactg ttaattgcgc acgaataagt aacgcatcaa
     3541 taccaccacg gacgaacatt aatacggcac agattaaata cataataccg attttcttat
     3601 ggtctacaga tgtgaaccat tctttgtaaa gatatttcca taatttaaag taagtaatta
     3661 ctgcgattaa accaataact aagaatgggg caccaatttg tgccattgta atcatccagt
     3721 tacctttaac tagtaattga tcccatggaa aattcattaa tgtccacctc catgatcatc
     3781 attgtcttga tcttgcgcat cttttgaaat tttcttcatt tctttcgcat ttttcgattc
     3841 atctttcttg aactcattgt tatatggttc gtcatttcca agaatcatca acttcatacc
     3901 atgtcgttta tagttcgcat ttgtaatttg agctttacga gcaggtatta atggtttgtc
     3961 tgatacatct ttaaacatat tttcttcact agtgaagttt ggatctttta attcgaaatt
     4021 gaaacgttta tatgcataga agatgtattc tggatcggct gctggatcaa caaacgccat
     4081 atgtgtacca ttaaattcta aagctttatt aggtgtgctt ggtaataatt gtttatcaaa
     4141 tgtatcttga tctaacgttt tcttaccttt aacttctttc acccatttgt cgtagtcttt
     4201 ttgactaacg gcatttactt taaatgtttg acgtgagaat ccttcaccat tgaagttaga
     4261 gttacgacct ctgaacgtac cagtttgaga tgcttctaac gtccaattca ttgtcatgcc
     4321 agtcatggca tatttttgac cacctaattg tggaatccag aaacttgtca ttgtatccat
     4381 agcttgaagc ttaaatacaa caggacgatc tttagggatt gttaatgtat taacagtctc
     4441 tatatgttca tctggataag caaagaacca tttgtatcct gcacttactg catatacaac
     4501 cattggatct ttctcactct tcggtggttt ttcgtaatcg tataaagttt taactgtagg
     4561 aatagctaaa gcagcaacga ttatgatagg tattacaaac catattgttt caatgatggc
     4621 attatggtgc atcttaccag attcggcatt cttattataa ctatacttgt aaataaaaat
     4681 ggcgaacatg ccaagtacaa cgaaacaaat aacaagcatg aagacgattg aataaagaat
     4741 caagaacttc tgactacttg ctactggccc ttttgcgttg aaaatttcta tatttgaaca
     4801 accactaagt aaaattagtg tgccaaataa tagaagcaaa gacttaaatt ttgacacttt
     4861 tttgacctcc taatactaca aatgtagggc ttaacattaa ttttaagtta ttacacaata
     4921 tttacaaggg cttatgggaa aaaaattaat aaaattgtat caaaaatgtt gataaatcaa
     4981 ggtgtgacgt gggttcacac atttgttaaa attatgtgta cattttgtga ctaatagcgt
     5041 ttttgaaaaa attgcttcat atttaaaagg aaagttgaga atagctttca attaaaggtt
     5101 aattgaatga aaataaaaaa agtttgaaat tgtctaataa attgaaaatg atggctagtt
     5161 gattgaaagg aagcgcaaac attttaataa ggaatttgcc aaaagtaatt tatcgttaaa
     5221 caatgaaaag tggaaatttg tttgttcaaa atcaaaacat ttttttaaaa agagcagtta
     5281 agttttaata atttgatagg tagtcctttg gtatacagaa aaaatacaat atttgggtta
     5341 aagttgcaga tgaagttaga ataacaaata aaatggtagc aaaacatgtt cgtttgctac
     5401 cattttattt gcagtatagt gatgagggct atttaacctc aatataccta gtggaaattt
     5461 taattgcacc atctgaatca gtgacatgat atttaatttg atatttacca gatttagatg
     5521 tatcaatttg gccatcgact ttaattttat cggttaaatc tccatcttct ttatcaaatg
     5581 cacttatgcc gtttagaagg ttataatctt gacctttctt aataacgata tcattagcgc
     5641 ctttaatttg tggtgtagta ttcgtcgttg catctgcatt taaatttggt gttactagtg
     5701 tagcagaaac accgagggct gataatgact gtaatagttt attcataatg ta
//
LOCUS       DANJFZ010000142         5685 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.143, whole genome shotgun sequence.
ACCESSION   DANJFZ010000142 DANJFZ010000000
VERSION     DANJFZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.143"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(46..540)
                     /locus_tag="PWN45_002469"
     CDS             complement(46..540)
                     /locus_tag="PWN45_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624208.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(939..1361)
                     /locus_tag="PWN45_002470"
     CDS             complement(939..1361)
                     /locus_tag="PWN45_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0624209.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            1509..2225
                     /locus_tag="PWN45_002471"
     CDS             1509..2225
                     /locus_tag="PWN45_002471"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL0624210.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            2308..2847
                     /locus_tag="PWN45_002472"
     CDS             2308..2847
                     /locus_tag="PWN45_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL0624211.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(2997..3317)
                     /locus_tag="PWN45_002473"
     CDS             complement(2997..3317)
                     /locus_tag="PWN45_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0624212.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            3461..3817
                     /locus_tag="PWN45_002474"
     CDS             3461..3817
                     /locus_tag="PWN45_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL0624213.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3975..4355
                     /gene="gcvH"
                     /locus_tag="PWN45_002475"
     CDS             3975..4355
                     /gene="gcvH"
                     /locus_tag="PWN45_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDL0624214.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            4534..5412
                     /locus_tag="PWN45_002476"
     CDS             4534..5412
                     /locus_tag="PWN45_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDL0624215.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT         2013 a          788 c          996 g         1888 t
ORIGIN      
        1 tatatgattt gaatttatga gaaatgtaga atatataaaa ctgtattact tattaatcaa
       61 atcataaaaa agccaatgtt catcgaaata ggtatttccg attttaattg catttttctc
      121 aaaaccaaga atgtcgaatc ctagtgcact atagaaaact ttagcaccta tgttgtttga
      181 cacaattgat gtaagtaaag attcgtaatt gttctgccgt gcataattaa taatgaaatt
      241 aatgagctcg ctattaatcg atttatcgtt attagtaaca aagttgtatt taattaatga
      301 tttatgttct tttccaacgt atcgaatttg ttctaaggca gctgttgcga taagtgtttc
      361 gttttcataa cagccaaata caatacattt cggtgaacct tgatttaaaa tgtcatgtat
      421 taaggtgtca gtcattgttt gactataatg tgaatcttga gtgaattctt cattgactgt
      481 cgaaacaagt ctcttatact cattaaaatc attattcgtt aatgttttaa tttcaatcat
      541 tactgacctc cttaatcatt attaagaaag attttgtact aaaaaaattt cttaattaca
      601 tattaaaatg tttattaaaa ttttgcaatc actgatttat atgcacattc ttgtaaatgg
      661 attatcaatt taattatatt tatttttaag ttcaggaaat tatggaattg gttattggtc
      721 gacactggac ttttatttgt agacggtatg cgaagaagtc aagccagtat tggaaccggt
      781 cacatttatg atgactaagg tacaatcatt ttcagttaaa tattgccaga aatttcaatc
      841 acgaatagaa aaaataacca accaaaaatt tttggttggc aaatttgaat tttatgtgaa
      901 ttttattaat aacgttgaat aatctcttta agttaatgct aatctacaac atttacattt
      961 aaatcgacat taatatttcc ttgagtcgct tttgaatatg gacaaaattc atgagccatt
     1021 tgtaaatatt tttcagcttc ttcttgagat ataacatttt taattgtcgc atcaattgaa
     1081 acacttaatt taggactttc tgagtctgaa tcatcttcta gtctcactgt tagtgttact
     1141 tctggatgag catcacgcac tttgttttgc tttaaaatta ggtcgaaagc accgttgaag
     1201 caagatgcat aacctgctgc aaataattgt tcggggttag tagctttacc atctgcttga
     1261 gcaggcggaa cgatatcaat atctaatgct cgatcatcag tataaacatg tcctttacgt
     1321 ccgccaacat tcgtcgcttt agtttcataa tgtattgcca tacttataac ctcctaattt
     1381 aaaatacatt tatgctttac ccattcgaaa ataccttaat cattttcatt tatagcattg
     1441 tttgattgaa ggataaaaag ttgttgttta caataaaaat aatgagtatc tgaaatgagg
     1501 gattcactat gacacatgtg gaagtagtag cgactatcgc gccacaatta tctatcgaag
     1561 aaactttaat tcaaaaaatt aatcatcgta ttgatgcaat agacgtatta gaattacgaa
     1621 ttgatcaaat tgaaaatgtc acagttgatc aagtggcaga aatgattaca aagctgaagg
     1681 ttatgcaaga ttcattcaaa ttattagtta cgtatcgtac aaagttacaa ggtggctatg
     1741 ggcaatttac aaatgactcg tatcttaatt taatatcaga cttagcaaat atcaatggca
     1801 tagatatgat tgatatagaa tggcaagcag atattgacat tgaaaaacat caacgaatca
     1861 ttacacattt gcaacagtat aataaagagg tggttatatc acatcataat ttcgaaagta
     1921 cgcctccatt agatgaattg caatttatat tttttaaaat gcaaaaattc aacccagaat
     1981 acgttaaatt agcagtaatg ccacataata aaaatgatgt gttaaattta ttgcaggcaa
     2041 tgtctacatt ttcagatact atggactgca aagttgttgg tatttcaatg tctaaacttg
     2101 gactaataag tagaacggct caaggcgttt ttggtggtgc gttgacttat ggttgtatcg
     2161 gagtaccaca agctccagga cagattgatg ttactgattt aaaagcacaa gtgactttat
     2221 actaataaaa tgaattaggt tgcttttgtg taagtgctgt cttataattg agaagtatta
     2281 tcattgttcg tggaggagcg attttagatg gaattacaac aagcaatagc taatagaaga
     2341 agtgtgaaaa aatttaaaag agatatgcac atagatgacg cattgctata tcaagcaatt
     2401 gagaaagctg ctgatgctcc aaatcacgga atgagggaac catggagagt tgtgcatgtt
     2461 ccgaaagaca gattaggaga tatgagtaag gatatttcta aatttgcatt tcctaatgaa
     2521 ttagataagc aacaatgtca ttatgatgca gttacgaaac taggtggcat gttattgctt
     2581 attttaaaaa cagatccaag acaacgtcaa aatgatgaaa actactttgc atttggtgca
     2641 tatgcacaaa atcttatgtt gttactttat gaagcgggaa taggtacatg ttggaaatcg
     2701 ccattatata tctatgatcc taaagtaaga aaaacacttg gtataaagaa agatgaagtt
     2761 cttgctggat tcttatattt aacggattta gaaggagata tgcctaaagc accacgtaaa
     2821 aatagaaact taattacatt atattaatat gtataattat agaaacatta ataaaagctg
     2881 agtcatgaat tgatggacat ctatcgagtt agagatttaa tctaacttac tagagtcggt
     2941 acaataacag tctcagcttt ttattgtgca gtatatacac atttttattt tagtatttat
     3001 ttaaaagttt ctgctaaaaa tgattcaact tgttcaggtg acttagcatt tgctgaatga
     3061 aggtgtgcaa ttttatcgcc gtttttaaat actagcaagc tagggatacc cataacttca
     3121 ttttcaacaa ctacatcttc taattcatca cgattaacag tataccattg gtaatcatta
     3181 tattgttcta cgattgggtc aatccataaa tccatagcac gacagtctgg gcaccatcct
     3241 gcctcaaatt taacaattac aggtgtatcg ctattaatta cagatttaaa tgattcatta
     3301 cttttgattg attgcattgt aacaactcct ctagatagtt taataatttt tattatagct
     3361 aaatttatat cataataaaa aattttagct tcaaaatgaa agccttttgc tttggaaaat
     3421 gatatattta ttttaaatac ataaaggagg ttgcagtcgt atgattaaat tttaccaata
     3481 taagaattgt acaacttgta aaaaggcagc aaagttttta gatgaatatg gcgtaagtta
     3541 tgaaccaatt gatatcgttc aacatacacc tacaataaat gaatttaaaa caataattgc
     3601 aaatacaggc gtagaaatta ataaattgtt taatacacac ggcgcgaaat atcgtgagct
     3661 tgatttgaaa aataaattac aaactttatc agatgatgaa aagttagagt tgttatcatc
     3721 tgatggtatg ttagtaaagc gtcctctagc agtaatgggc gataagataa cattaggatt
     3781 taaagaagat caatataaag agacttggtt agcgtaagtg aaatgtaagc gtttactaaa
     3841 tatctcgata tttagattca ttacatgtaa aatgaaataa gctatacaat tgttaatttt
     3901 tataaatata gttgaatagc atctagcctt atggcatcat taatgatgta aagattaatt
     3961 aggaggggat tctcttggca gtaccaaatg aattgaaata ttcaaaagag catgaatggg
     4021 ttaaagttga aggtaatgta gcaataattg gaatcacaga atacgcacaa agcgagttag
     4081 gtgatattgt tttcgttgaa ttaccagaaa cagatgatga aattaatgaa ggggatacgt
     4141 ttggtagcgt agaatcagtt aaaactgtat cagaattata tgcaccaatc tctggtaaag
     4201 tagttgaagt caacgaagaa ctagaagata gtcccgaatt tgtaaatgaa tctccatacg
     4261 aaaaagcatg gatggtaaaa gtagaaatta gtgatgaaag tcagattgaa gctttattaa
     4321 cagctgaaaa atattcagaa atgattggtg aataatcacc gtgtaactcc ttaatctaag
     4381 attgaggagt ttatttttag tctgagaaaa taaatgatat gaaagaaaaa ttattaggta
     4441 ctattatttg gagtattgct acattttatt attcaagaat gatggaaata atgaatttag
     4501 ctattttaaa aataaaaatt gggggaagtt aatatgctaa acattcaaga cgttagtcat
     4561 ctttctaaaa aggagcaaaa agcatataac cgtttcgtag aatctgtaga aaacggtaat
     4621 ttaccagtac taccatgtat tgaaatggat ctaaaagaga tgcaagaaga aacattaaac
     4681 cagagtaaga ttggtggaat gccattttta aaatatttta aagatatacc attagatgaa
     4741 aataatgtac caatggtatt gctagcacag attaatttgg atgatcttcc agaacaacaa
     4801 gaattatttc ctgtaaaaga agggatattg cagttttgga ttagttcaga agatcaaatg
     4861 tatggaatgt ctgaaaattt aaagggaaac aatataaact caaggcttgt ttatataaaa
     4921 gagccaatta cagatttatc actcgaaaat attcaagcgc atttgaagtc attagatgct
     4981 gataatgagg atatcccgtt cagtggagca ttttctatag aatttagatt gtcgaaacaa
     5041 actattacat gtactgatta taagtacgat gaggacgtgc ttgcattgtg gaataaagtc
     5101 aatccatcct tcgcgctaaa atcaatgttt ggtggttatg atgaattgat ggaacctgtg
     5161 tgtaacacat ttactgctaa ggaaccattt aatcaacttg gtggttatcc atattttgac
     5221 caaatagatc caagaacgaa cgatcaagaa ctgaaaatgt atgatagagt cttactgcaa
     5281 attgattcta caagagatgg taattcttcg attatatggg gtgatttagg tattgccaat
     5341 atcttagtga aatctactga ccttgaggct atgaagtttg atgattacat gtattcatgg
     5401 gattgcagct aagagaatca taaaaatata ttactttaat aagtgtagta tctatagatc
     5461 aatctaggga gtgggacata aatgatattt tcgcaaaatt tatttcgtcg tcccacccca
     5521 acttgcattg tctgtagaaa ttgggaatcc aatttctctt tgttggggcc catccccaac
     5581 ttgcattgcc tgtagaattt cttttcgaaa ttctctgtgt tggggcccct gactagaatt
     5641 gaaaaaagct tgttacaagc gcattttcgt tcagtcaact actgc
//
LOCUS       DANJFZ010000143         5632 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.144, whole genome shotgun sequence.
ACCESSION   DANJFZ010000143 DANJFZ010000000
VERSION     DANJFZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.144"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            335..799
                     /gene="accB"
                     /locus_tag="PWN45_002477"
     CDS             335..799
                     /gene="accB"
                     /locus_tag="PWN45_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL0624216.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            799..2154
                     /gene="accC"
                     /locus_tag="PWN45_002478"
     CDS             799..2154
                     /gene="accC"
                     /locus_tag="PWN45_002478"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL0624217.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2169..2531
                     /locus_tag="PWN45_002479"
     CDS             2169..2531
                     /locus_tag="PWN45_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0624218.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2591..2980
                     /gene="nusB"
                     /locus_tag="PWN45_002480"
     CDS             2591..2980
                     /gene="nusB"
                     /locus_tag="PWN45_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL0624219.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2997..4334
                     /locus_tag="PWN45_002481"
     CDS             2997..4334
                     /locus_tag="PWN45_002481"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL0624220.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4327..4557
                     /locus_tag="PWN45_002482"
     CDS             4327..4557
                     /locus_tag="PWN45_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL0624221.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4535..5416
                     /locus_tag="PWN45_002483"
     CDS             4535..5416
                     /locus_tag="PWN45_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0624222.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         2161 a          775 c         1054 g         1642 t
ORIGIN      
        1 ttaaaatata aaacgaatcg tctaattggt agtgaaaaaa catagacatg agtagaggta
       61 tatatctaca tatcatattg ttgaggataa aacataatag ctaataaaaa taacattgat
      121 atgtttaact gaatgcataa aatttttaat caagagttaa gatgagtcaa tgttaagtaa
      181 taaatacaca agtgttttta atttaaaatg tggttttatt ataactttgg gatagactta
      241 taatgtcgcc agcaatatga acatgcttga attgaagagt tgtctcaagt aaaatagacg
      301 ggtagatgaa aacaaactga aggagtcagt aataatgaac tttaaagaaa tcaaagaatt
      361 aattgaaatt ctggataaat caactttaac ggaaatcaat attgaagata ctaaaggcaa
      421 agtgacgctt aagaaagaaa aagaaactga gattatcacg ccacaaatct cacaaatgcc
      481 agttgaagct gcggcaatgc ctatgcctca agcacaatca actgatagca ataaaactga
      541 agctccaaag ccaacttcag ataatcacaa aacaattaat gcacctatgg taggtacatt
      601 ttacaaatcg ccatctccag acgaagaagc atatgtgcaa gttggggaca ctgtttcaaa
      661 tgaaacaaca gtgtgtattt tagaggcaat gaaactattt aatgaaattc aagcagaaat
      721 ttcaggtgaa attgttgaaa tcttagtaga agacggacaa atggtagagt atggccaacc
      781 gttatttaag gtgaaataat gaaaaaggtt ttaattgcaa accgcggtga aatcgcagtt
      841 aggattattc gcgcttgtcg tgatttaggc atccaaactg ttgcaatcta ttctgaaggg
      901 gataaagatg cgctacatac tcaaattgct gatgaagcat attgcgtagg tcccactttg
      961 tctaaagatt catatttaaa tattccgaac atcttatcta ttgcaacttc tacaggttgt
     1021 gatggcgttc atccgggtta tggcttttta gctgaaaatg ctgattttgc agaattatgc
     1081 gaagcatgcc aattgaagtt cattggacca agttatcaat ctatccaaaa aatgggtatc
     1141 aaagatgttg ctaaggcaga aatgatcaaa gccaatgttc cagttgttcc tggtagtgac
     1201 ggtttaatga aagacgtctc agaagctaag aaaatcgcca aaaaaattgg ctatccggtc
     1261 atcattaaag ctactgctgg cggtggcgga aaaggtatcc gtgttgctcg tgatgaaaaa
     1321 gaacttgaaa ctggcttccg aatgacagaa caagaagctc aaactgcatt tggtaatggt
     1381 ggactttata tggagaaatt catcgaaaac ttccgccata ttgaaatcca aattgttggg
     1441 gacagctatg gtaatgtaat tcatttagga gaacgtgatt gtacaattca aagacgtatg
     1501 cagaaattag tggaagaagc accttcccca attttagatg atgaaacacg tcgtgaaatg
     1561 ggaaatgccg cagttcgtgc agcgaaagct gtaaattatg aaaatgcggg aacaattgag
     1621 tttatatatg atttaaatga taataaattt tattttatgg aaatgaatac acgtattcaa
     1681 gtagaacatc ctgtaactga aatggtaaca ggaattgatt tagttaaatt acaattacaa
     1741 gttgctatgg gtgacgtgtt accgtataaa caagaagata ttaaattaac aggacacgca
     1801 attgaattta gaattaatgc tgaaaatcct tacaagaact ttatgccatc accaggtaaa
     1861 attgagcaat atcttgcacc aggtggatat ggtgttcgaa tagagtcagc atgttatact
     1921 aattatacga taccgccata ttatgattcg atggtagcga aattaatcat acatgaaccg
     1981 acacgagatg aagcgattat ggctggcatt cgtgcactaa gtgaatttgt ggttcttggt
     2041 attgatacaa ctattccatt ccatattaaa ttattgaata acgatatatt tagaagcggt
     2101 aaatttaata caaacttttt agagcaaaat agcattatga atgatgaagg ttaataggag
     2161 gtcaatccat ggtcaaagta actgattatt caaattcaaa attaggtaaa gtagaaatag
     2221 cgccagaagt gctatctgtt attgcaagta tagctacttc ggaagtcgaa ggcatcactg
     2281 gccattttgc tgaattaaaa gaaacaaatt tagaaaaagt tagtcgtaaa aatttaagcc
     2341 gtgatttaaa aatcgagagt aaagaagatg gcatatatat agatgtatat tgtgcattaa
     2401 aacatggtgt taatatttca aaaactgcaa acaaaattca aacgtcaatt tttaattcaa
     2461 tttctaatat gacagcgata gaacctaagc aaattaatat tcacattaca caaatcgtta
     2521 ttgaaaagta atgtcatacc taattcagta attaaataaa gaaaaataca aacgtttgaa
     2581 ggagttaaaa atgagtcgta aagaatcccg agtgcaagct tttcaaactt tatttcaatt
     2641 agaaatgaag gacagtgatt taacgataaa tgaagcgata agctttatta aagacgataa
     2701 tccagattta gacttcgaat ttattcattg gctagtttct ggcgttaaag gtcacgaacc
     2761 tgtattagac gagacaatta gtccttattt aaaagattgg actattgcac gtttattaaa
     2821 aacggatcgt attattttaa gaatggcaac atatgaaata ttacacagtg atacacctgc
     2881 taaagtcgta atgaatgaag cagttgaatt aacaaaacaa ttcagtgatg atgatcatta
     2941 taaatttata aatggtgtat tgagtaatat aaaaaaataa aattgagtga tgttatatgt
     3001 cagattattt aagtgtttca gctttaacga aatatattaa atataaattt gatcaagatc
     3061 cacatctcca atctgtattg attaaaggtg agctttcaaa tttcaaaaag cattcaagtg
     3121 gtcatttata ctttaatgtg aaagataaag aaagcgttat aagtgccatg atgttcaaag
     3181 gtagtgcttc taaattaaac ttcgaaccta aagaaggaga tgaagtctta ttagaagcac
     3241 gtgtttctgt ttttgaacgt cgtggaaact atcaaattta tgtaaataaa atgcaattag
     3301 atggtatagg gaacttatat caaaaattag aagcattaaa gaaaaaatta actgaagaag
     3361 gttgttttga taaagcgaat aagaaatcaa tacctaagtt tcctaaaaaa attgcagttt
     3421 taacagcgag tacaggtgcc gcaattcgag atatccattc aacgataaat agtcgttttc
     3481 cattagctga acaaatacaa attagtactt tggttcaagg tgaaaaagca aaagacgaca
     3541 ttattgaaaa aattgaatat gcagatagtt taggtgtaga taccattatt gtaggtcgag
     3601 gcggtggatc catagaagat ttatggaatt tcaacgaaga agctgtcgtc cgtgcaatat
     3661 ataattgtaa gacaccgatt atatcagcag ttggtcatga aacagacttt acattaagtg
     3721 attttgctgc agacatcaga gctgcgactc caactcaagc tgctgttatt gcaacaccag
     3781 accaatatga attgctgcaa caaatacagc aatatcagtt tacattgact cgtttcatca
     3841 agaaacattt agaacaacaa cgtaaacatg ttgaacattt gtcatcgtat tacaagttta
     3901 aacaaccaac tttattgtat gatcaacaga tacaacgtcg agatgattta gaaaagagac
     3961 tgaaacaaca aattcaggca acttttgaac aacagagaca tcgattaatg ctgttgcaac
     4021 aacgttataa tttaaaagca ttactaagta gtgttaatca agaacaacaa aataatcttc
     4081 aattgacaaa ccaacttgtt aaattattga attctaagat attaagttat aaaaatgatt
     4141 tgaaaaataa agtagaaaac ttaaacaatt taagtccaac taatacaatg ttgcgtggat
     4201 atgcaattgt taataaaaaa gacgaagtca ttacgagtac taaagattta acagaaaatg
     4261 atcaattgac attaacaatg aaagatggct tagtagatgc aaaagttacg aaagtaaggt
     4321 gtaataatga ctaaagaaac gcaaagtttt gaagaaatga tgcaagaatt agagcaaatt
     4381 gttcaaaaat tagataatga aacagtatct ttagaggaat cattagattt atatcaacgt
     4441 ggtatgaaac tatcagcagc ttgtgacaca actttaaaaa atgccgaaaa aaaggtgaat
     4501 gacttaataa aagaagaagc tgaggatgta aaaaatgacg aatctaccga tgaataaatt
     4561 aatagatgaa gtcaataatg aattatcggt tgcgataaat aaatcagtaa tggatactca
     4621 gctagaagaa agtatgttgt attcattaaa tgctggaggt aaacgcatcc gaccagttct
     4681 gttattactc actttagatt cactaaatac cgagtatgag ttaggtatga agagcgcaat
     4741 tgcactagaa atgattcata catattcact tattcatgat gacctaccag cgatggataa
     4801 tgatgattat cgacgaggaa aattaacaaa tcataaagta tatggtgagt ggactgcgat
     4861 attagcaggt gatgctttat taactaaagc atttgaactt atttcaagtg atgatagatt
     4921 aactgatgaa gtaaaaataa aagttctaca acggctgtca atagcaagtg gtcatgttgg
     4981 aatggtcggc ggtcaaatgt tagatatgca aagcgaaggc caaccaattg atcttgaaac
     5041 tttggaaatg atacacaaaa caaaaacagg agcattatta acttttgcgg ttatgagtgc
     5101 agcagatatc gctaatgtcg atgatacaac taaagaacat ttagaaagtt atagttatca
     5161 tttaggtatg atgttccaga ttaaagatga tttattagac tgctatggtg atgaagcaaa
     5221 gttaggtaaa aaagtgggca gcgatcttga aaataataaa agtacgtacg tgagtttatt
     5281 agggaaagat ggcgcagaag ataaattgac ttatcataga gacgcagcag tggatgaact
     5341 aacgcaaatt gatgaacaat tcaatacaaa acacttatta gaaatcgttg atttatttta
     5401 tagcagggat cactaaaagc aaatatatcg attagcaaca attgtaaagt ttgaaatgtc
     5461 tcaaaaggca tattcaactg acgttgtttg ttaatcgatt tttctcatgt tgaaacttaa
     5521 aattatgttg aagactttca tttaaaaaat agaattttca atttctatac gacaatattc
     5581 gtatctgtga tatcaatgtg attttaatta aatatgttca aacaattgac gc
//
LOCUS       DANJFZ010000144         5506 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.145, whole genome shotgun sequence.
ACCESSION   DANJFZ010000144 DANJFZ010000000
VERSION     DANJFZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.145"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            120..2567
                     /locus_tag="PWN45_002484"
     CDS             120..2567
                     /locus_tag="PWN45_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDL0624223.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(2676..2774)
                     /locus_tag="PWN45_002485"
     CDS             complement(2676..2774)
                     /locus_tag="PWN45_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624224.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            2908..3198
                     /locus_tag="PWN45_002486"
     CDS             2908..3198
                     /locus_tag="PWN45_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL0624225.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            3179..4546
                     /locus_tag="PWN45_002487"
     CDS             3179..4546
                     /locus_tag="PWN45_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0624226.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            4559..4996
                     /locus_tag="PWN45_002488"
     CDS             4559..4996
                     /locus_tag="PWN45_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0624227.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            5153..5467
                     /locus_tag="PWN45_002489"
     CDS             5153..5467
                     /locus_tag="PWN45_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0624228.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
BASE COUNT         2106 a          747 c         1080 g         1573 t
ORIGIN      
        1 aaaattaaag ttctcaagtt ttcattttcg tatttatcat aaatgatttc gcgtaaagtt
       61 ttcaaaattt gtcacctctt taattttatt aaattaatta tacaagaaag gagccgaata
      121 tgttagacaa agtcactcaa atagaaacaa ttaaatatga tcgtgatgtc tcatattctt
      181 atgctgctag tcgtttatcc acatattgga ctaatcacaa tatggcttgg tctgacttta
      241 tgcagaagct agcacaaaca gttagaacta aagaagattt aactgagtac aataaaatgt
      301 ctaagtctga acaagcagat ataaaagatg ttggcggatt tgtcggtgga tatttaaaag
      361 aaggcaaacg acgtgctggt caagtcatga atcgttcaat gctaacactt gatatcgatt
      421 atgctgctca agatatgact gacatattat ctatgtttta tgattttgca tattgtttat
      481 attcaacaca taagcataga gagataagtc caagactgcg tttagtgatt cctttaaaac
      541 gaaatgtaaa tgcagatgag tatgaagcta ttgggcgtaa agtcgcagat atcgttggca
      601 tggattactt cgatgataca acttatcaac cacataggtt aatgtattgg ccttcaacta
      661 gcaatgatgc agaatttttc tttacctatg aagatttacc tttgttagat ccagataaaa
      721 tattaaatga atatgttgat tggactgaca cattagaatg gccaacgtct tcaaaggaag
      781 agagtaagac taaaagatta gcagataagc aaggtgaccc agaagaaaag ccgggaattg
      841 ttggcgcatt ttgtagagcc tatacgatag aagaagctat atcaactttt attcctgact
      901 tatacgaaaa acattctact aaccgttata cctatcatga aggttcaact gcaggtggat
      961 tggtgttata cgaaaataac aagtttgcct attctcatca taatacggat cccgttagcg
     1021 gtatgcttgt gaacagtttt gatttagtac gcatacactt atatggtgct caagatgaag
     1081 acgctaaaac agatactccg gttaatcgac tacctagtta taaagcaatg cagcaaagag
     1141 cgcaaaatga tgaagttgtt aaaaagcaat taattaacga caaaatgtct gatgcaatgc
     1201 aggatttcga tgaaatagta aatagcgatg atgcatggtc tgagacgtta gaaattactt
     1261 cgaaaggtac tttcaaagct agtatcccaa atatagaaat tatattgcgt aatgatccaa
     1321 atttaaaagg aaaaatagca tttaatgaat ttacaaaaca aattgaatgc ttagggaaag
     1381 tgccatggaa tactaatttt aagacacgtc aatggcaaga cggtgatgat agcagtttaa
     1441 gaagttatat cgaaaagatt tatgacatac accattcagg taaaacaaaa gatgccatta
     1501 taagcgtagc aatgcaaaat gcttatcatc cagtaaggga ttatctaaat aaaatatcgt
     1561 gggatggaca taaacgtctt gaaaagttat ttatcaaata cttaggtgtt gaagatactg
     1621 aagtgaatag aacaactacc aaaaaagcat tgactgctgg aattgctcga gtaatggagc
     1681 ctggatgtaa atttgactat atgcttacac tttatggtcc tcaaggtgta ggtaaatctg
     1741 ctttgctaaa aaaattaggt ggtgcatggt tttctgacag tttagtttct gttacaggta
     1801 aagaagccta tgaggcctta caaggcgttt ggctaatgga aatggcagaa cttgcagcta
     1861 caagaaaagc tgaagttgaa gctattaagc atttcatatc taaacaagtt gaccggtttc
     1921 gtgttgctta tggacattat attgaagatt ttccaaggca atgtattttc attggtacaa
     1981 ctaataaagt tgatttctta agagatgaaa ctggtggaag acgtttttgg ccaatgactg
     2041 taaatccaga gagagttgaa gtgaactggt ctaaactaac caaagaagag atcgaccaaa
     2101 tttgggcaga agctaaatat tattatgaac aaggagaaga gttattcctc aaccctgaac
     2161 tagaagaaga aatgcgttca atacaaagca aacatactga ggaatctcca tatacaggca
     2221 ttattgatga atatcttaac acaccaattc ctagcaattg ggatgactta actatctttg
     2281 aacgaagacg attttatcaa ggtgatgttg atatgttacc aacaggaaat gtagattacg
     2341 ttgaaagaaa taaggtctgt gcgcttgaag tgtttgttga atgttttggt aaagataagg
     2401 gagatagtag aggatctatg gaaattagaa agatttcaaa catcttaaga caattagaca
     2461 attggtctgt atatgatggt aataaaagtg ggaaaattcg atttggaaaa gattatggtg
     2521 tacagatagc ttatgtaaga gatgaaagtt tagaagattt aatataataa atattgaata
     2581 aatatacatt ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga tgcaacacgt
     2641 gagtgtaaaa agtaatcgta ggtgttgcat catttttagt gatgcaacat tgatgcaaca
     2701 aatgatacaa cacctctttc ccttctcgct gtaaggttca accctgtttg tttccaatgt
     2761 tgcatcaaat tcactataaa gtttaaaaag tagtgttagg gagtaaagag gtataggggt
     2821 aaccctctaa cagctatttt taaaagtttg gcaagaattg atgcaacatc ggaacacaaa
     2881 tataaatttt gtatacaagg tgaatatatg aaagaatcga cattagaaaa atatttagtg
     2941 aaagagatat caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg aacaagaggt
     3001 gtgccagata gaattattat tatgccagaa ggaaaaacat attttgtaga aatgaagcaa
     3061 gaaaaaggaa agttgcatcc tttacaaaaa tatgtgcata gacaatttga aaatagagat
     3121 cataaagtat atgtgttatg gaataaagaa caagtaaata cttttatcag aatggtaggt
     3181 ggaacatttg gcgattgact tcaaaccaca tagctatcaa aagtatgcaa tagataaagt
     3241 gatagataat gagaaatacg gtctgttttt agatatgggt ctagggaaaa cagtatcaac
     3301 acttacagca tttagtgaat tgcagttgtt agacactaaa aaaatgttag ttatagcacc
     3361 taaacaagtt gctaaagata catgggttga tgaagttgat aagtggaacc atttaaatca
     3421 tctgaaagtg tctttagttt taggaacacc taaagaaaga aatgatgcat taaacacaga
     3481 ggctgatatc tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc aatataaaaa
     3541 agaatggcca tttgacatgg ttgtgattga tgaactgtct acatttaaaa gtcctaagag
     3601 tcaaaggttt aaatctatta aaaagaaatt accactcatt aatagattta taggattaac
     3661 aggaacacct agtccaaata gtttacagga tttatgggct caagtttatt tgatagacag
     3721 aggtgaaaga cttgagtctt cattcagtcg ttatcgagaa aggtacttta aaccaactca
     3781 tcaagttagc gaacatattt ttaagtggga gctaagagac ggatctgaag aaaagatata
     3841 taaacaaata gaagatatat gtttaagcat gaaagcgaaa gattatctgg atatgcctga
     3901 cagagttgat actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag tatatgaaga
     3961 attagaaaaa aactatattt tagaatcgga agaagaagga acagttgtag ctcaaaatgg
     4021 ggcatcatta agtcagaaac tacttcaact atctaacggc gcagtttata cagatgagga
     4081 agatgtaaga cttatacatg ataagaagtt agataagtta gaggaaatta tagaggagtc
     4141 tcaaggccaa ccaatactat tgttttataa cttcaaacac gataaagaaa gaatacttca
     4201 aaggtttaag gaagcaacca cattagacga ttcaaactat aaagaacgtt ggaacagtgg
     4261 agacattaag ttgcttatag cacatccagc aagtgcagga catggattaa acttacaaca
     4321 aggtgggcac attattgttt ggtttggact tacatggtcc ttggaattat accaacaagc
     4381 aaatgctaga ttatacagac aaggacaaaa tcatacgact attattcatc atatcatgac
     4441 cgataacaca atagatcaaa gagtatataa agctttacaa aataaagaac taacgcaaga
     4501 agaattaatg aaagctatta aagcaagaat agctaagcat aagtaatgga ggtctaacat
     4561 ggggaacaca atatatgata tcaagccagg cacatttaaa tatattgaat cagaaatata
     4621 taatttaaat gagaacaaga aagaaataaa aagattgagg ttggaaatac ttaatccaac
     4681 gaaggaacaa gattccaata ttgtatatgg accattacaa aaaggcgaac cagttagaac
     4741 aactgaacta atggcaacta gattattaac taataagatg ttacgaaacc ttgaagaaat
     4801 ggtcgaagca gttgagagtg aatacttaaa gttgcctgaa gatcataaga aagtaataag
     4861 gctcaagtat tggaataaag aaaagaagtt aaagatggaa cagataggac atgaatgcca
     4921 tatgcatcgt aatactgtta ctactatacg aaagaacttt gttaaagcgg tagcgtatca
     4981 tgcaggtatc aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat ctgtagtaat
     5041 atgatagtat cggaaagatg tataaagtta tctaaaagtt atacgacaca agtacacgag
     5101 gcacatcgct atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag agatgaccaa
     5161 gcacaataac atttataagc atggtcgtaa gtcatatcaa tacgattggt tctatcattc
     5221 aaaagcatgg aagaagttaa gggagatagc attagataga gataatcatc tttgtcaaat
     5281 gtgtttacgc gaagatattg taacagatgc aaacatagtg catcacatta tttatgttga
     5341 tgaagatttt aacaaagctt tagaattaga taatctaatg tcagtttgtt atggctgtca
     5401 taacaaaatt catgcaaatg gtaatgacaa aagtaatctt aagaaaatta gagttctaaa
     5461 aatttaaata aaaaaacatt taaataaaat tttatagccc cctgcc
//
LOCUS       DANJFZ010000145         5470 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.146, whole genome shotgun sequence.
ACCESSION   DANJFZ010000145 DANJFZ010000000
VERSION     DANJFZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.146"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..167
                     /locus_tag="PWN45_002490"
     CDS             <1..167
                     /locus_tag="PWN45_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HDL0624229.1"
                     /translation="RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEES
                     GIADGTEEIE"
     gene            145..459
                     /locus_tag="PWN45_002491"
     CDS             145..459
                     /locus_tag="PWN45_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDL0624230.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            474..1991
                     /locus_tag="PWN45_002492"
     CDS             474..1991
                     /locus_tag="PWN45_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624231.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            2000..2836
                     /locus_tag="PWN45_002493"
     CDS             2000..2836
                     /locus_tag="PWN45_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624232.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(3097..4074)
                     /locus_tag="PWN45_002494"
     CDS             complement(3097..4074)
                     /locus_tag="PWN45_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0624233.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            4226..5263
                     /gene="mutY"
                     /locus_tag="PWN45_002495"
     CDS             4226..5263
                     /gene="mutY"
                     /locus_tag="PWN45_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL0624234.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         1979 a          742 c          947 g         1802 t
ORIGIN      
        1 aacgagattt agaaaaaatt tataataaaa atcgaaagaa gtacacgcaa cagaaattaa
       61 tttcaaaaac tatcgaaggc cttatgagaa aaggatataa atatgataaa attaaagcta
      121 aattagaaga aagtggtatt gccgatggaa cagaagaaat tgagtgagat gtctgagcca
      181 gaattaagac acgaaataca actttataaa gagaaaatga gaaaagctga gatgaatggt
      241 attttaaatg aatatgacgt ttatcaaagt aaagtaattg ttgccgaaag ttacttagta
      301 gacagaaaaa agatagaaat tggtaaaatt tataaactga ctgatggcag taatcaatat
      361 tttaaagtag aaagattaaa aggtattttt gcgtgggggt ttagatttaa tagcgatgaa
      421 cccgaagaag gattacctat agcgttatta caattataga aggatgaaaa tagatgggaa
      481 gttcaattgt tttaaagtta ctaaaagtaa cacactacta taggaataaa cagaataaga
      541 aatggtattt accttttgga tatgatgctg aagatatcga tttaaacaat attagtttac
      601 atatttatca aggagaagca ttaggtataa ttggtgaacc tgaatcttcc aaagcgttgg
      661 taggtcaatt gttggcaggt gcaattaaac ctgataaagg taaagtagtt tgtactgaag
      721 atttgttcta cggatatatt gaagatcaat cgttaattca tcaaactgtt gaagcttata
      781 cagcgcagtt agttcaacta tttccatatg aaattaatga tcataaagct gaacagatta
      841 ttcaatatgc acatttaggt gattataaaa cgaagccggt taaccatatt tcgaaagcgg
      901 catacgctca attactatta agtattgcac gctcatcaaa atcaaatatt attattttaa
      961 atcatgttat tgactattta acaccacaat ttatggaacg tgcgattgaa ttaacaaatg
     1021 attatattga aaataattta acgattgtgt caattggtga tgatattgat aaaatttcac
     1081 aagtgagtaa ctacatagct tggttttcac atggtcaatt aagaatggaa gggtcactta
     1141 aacaagttat tccatctttt aaagaacatg aacgtgatcg attaagtcta aactcaaaag
     1201 aagaaattga aaactttgat ttagattgga aaaagaatcg tacaagaata ccagagatga
     1261 cctataattt caaacgtgtt gagcgctata atcatgcaaa accgcctaag tttttagtgc
     1321 gtttttggac tttggcctca ggtactattt taggcttagc attgatgatg ttgctcattt
     1381 tcaataatat aggaattatt tcgataacag attttacgaa tcgtgctacg atgcaaaatg
     1441 aaaataaaga tccatatggc gaaaagttag cttatggaat tgcttttaat ggcagtgtgg
     1501 atatgcaagg ggataaacaa gtcacaattc caaaatatag tgtagttaca attactggcg
     1561 aaaatagtaa aaattatcgt gttaccgccg ataataagac ttactatgtt agtaaagata
     1621 aattagaata ttttaacccg gcaggtttat atcaaacgca tagttttaaa aaattagcac
     1681 catatatgaa atcaaattat agtaattact atgcatactt taatagtcaa ttacataaaa
     1741 agcatagttc agttataaaa actttagttc ctgatgatga taaccgtttc gttgcatccg
     1801 ttacacaaca accgatacaa ttacttttca atgataataa tcagttatac ggttttgttt
     1861 atccaattgt agataaaaaa gaattaaaag ataagtttaa tattaacaat aacatttgga
     1921 ttactaaagt tgggaatgga tattgtattg ccaatttgaa agaagacaaa tggatttata
     1981 ttgaattgta ggtgtaaaga tgctagataa tattatttta tattttaaaa acttaccgca
     2041 tactaaaagg tatgtaacag aacgtttaaa acaaagttgg aaaagttttc taatcgtatt
     2101 agctgcatgt ttaatactta ttattgcaag tgaaacgcta ttttccttta gtcatttgac
     2161 cgatgttaaa gaggtgagat ggttatttag aatcattgta ttgattgttt ttgcggtagt
     2221 gatgttcaca atttatatct cttatcatca ttatatgaat gactttttag ttactaagtt
     2281 atttaatatt tccgcagcga cgccagtagt tattatgtct attttaagtt ttattatgct
     2341 agtcattttg acgatgatta gtgcattggt taaaccagta aacttcgaaa catcatatat
     2401 agcgctattc tattttattg tgatggcaac tatttttgta ggattaatat ccgttacttt
     2461 tggtcttatt cgactgttaa ctgaaaaaat aaatatcata ttttatgggg tttgtgtatt
     2521 gtgtttctta ttgttaccaa ttattttcat accaaatcca aatcatgtat ttatcaatca
     2581 catcctaatg ctaaatccaa tgtattacat tgttaatggt atagcacaat ctatcatatt
     2641 tggtatatca agtatggaaa acattccata tcatttttac tttattttat tcttatgttt
     2701 aatagctgca gtaaatttcg tattagcaag gtatacgaca cacgcaattt ataataaaac
     2761 atctaaagtg acacaaactg ataatcaaca ggatgtttca aatgatagta cagacgaggc
     2821 ggatacttct tcatgatgtt tagctgacct gttgtgtaaa ttaatctgtg agaatcagaa
     2881 acaattagaa tatcatctag atattgtttt cctatactga ttttgatatt tcgagtcatt
     2941 aaaggtagtt tgttagcaac aatataaatt ttagattttg taaaagcatt catagtttta
     3001 cattaaaaaa ccggacagag taaaaatcgt tttgattttc attctgcccg gttgttcatt
     3061 ttttatagat taactttagt gaataataac ttgaaattat ttagcataca gtttacgttg
     3121 taacttatct tctgtaaata cccagccaat ataagagtgg tctatgacat tatcgtttgt
     3181 tacatgtgca attgccacaa atgaataatg atcatttttt aaataacgca aatcaatcaa
     3241 acgaatttca gtagaaccat ctgctaacgt tgttgtttgc caacgataga ttgatgaaaa
     3301 gtttaaaaaa gtacgtatat ctttattacc tttgactttc cataaaatgg agtctgttga
     3361 taatgtttga cgttccactt tatcactaaa caccacattc ctaccatatg cttttccaac
     3421 atagtcatgt gcatcagttt gtatcgcgac acgccattcc ataaatttta ttgttggcgc
     3481 aacaaatact ttaacagggt gatgctcttg ttgaattgct tttaaagctt gttgcttaat
     3541 tacggctctc attttaaaac gaatcatgta ataaatgatt agtaaagcga ttataggaaa
     3601 gaagactgca aatggatgca agcctataac ccataataca ataccaatac aaagaactgt
     3661 gaaaataata gggtcaaatg tgttaatcac acttaattga atccatttgt ttgtgatagg
     3721 tctaagcgct tgtgtaccat aagaattgaa tatatctaca aagacatgta aaaatactgc
     3781 gagctgagcc cacatccata catgaaatgg gttggttcca ctaaagaacg tgaatattaa
     3841 aaatgtaatt aaaattggcc atagtattgt gaaagggatg gaatgcgtga tacctctatg
     3901 atgcgaaata tatgttgcat tgtcctttaa tttaagaaca gtatccccat caggaattaa
     3961 tgaaccaacg ataagggttg tagctgttgc accaaacgta gaagccattg ctggatcttg
     4021 agttgcaagt gctgtaaggc ccaccccaat tgcgatatgt gttgcagtgt ccatatcagt
     4081 tcactcattt cataatatta tatatactat tataatccaa tttcgcaaat aactaaatta
     4141 acttacatac taaccatgat tttaatgtac aataaaaaca ttatgagtaa agacaattaa
     4201 ttttggtgag gaaagggttt tataaatgta tcagcagtct agttttaaag aaaatttgat
     4261 acactggttt gatgaaaatc aaagagaaat gccatggcga caaacgacca atccgtacta
     4321 tatttggtta agtgaggtca tgttacaaca gacacaagtt aaaacagtta ttgactatta
     4381 tcatcgtttt gttgaacgat ttccaactgt ggaagttttg agtcaagctt cggaagatga
     4441 agtgttaaag tattgggaag gcttaggata ttatagtaga gctcgaaatt ttcatacggc
     4501 gattaaagaa gttcatgata aatatgaagg cttagttccc aaagatcctg atcaatttaa
     4561 agcgttaaaa ggtgttggcc catacacaca agctgcagta atgagcattg catataatgt
     4621 accactagca actgtagacg gaaatgtttt tcgagtatgg tctagattga atgatgacta
     4681 tcgagatatt aaattacaat cgacgaggaa atcgtacgaa caagaattat taccgtatgt
     4741 tactacagaa gctggaacgt ttaatcaagc tatgatggaa ttaggggcgt taatttgtac
     4801 accaaaaaat ccattatgct tattttgtcc agttcaagaa aactgtgagg catttgataa
     4861 aggtacattt gaaaagttac ctgttaaatc taaaaatgta agtaaaaaag taattgagca
     4921 atccgtcttt ttaattagaa ataatcaagg tcaatatctg ttgcaaaagc gtagcgaaaa
     4981 attattgcat ggtatgtggc aatttcctat gtttgaaagt gagcatgcta gacgtaaaat
     5041 gactgaaaaa atcggtcatg acattcaacc agtagaaaca cctatttttg agttgaagca
     5101 tcaatttact catttaacat ggaagattaa agtatacgcc gtttcaggag caattaacat
     5161 tgaaacatta ccagatgaca tgatttggtt tgatttaagt gatcgagatc aatatacatt
     5221 ccctgtacct atgtcaaaga tttatcaatt tataaatggg taacattcga tgattggtaa
     5281 tattatcgga aaacatagtg acattaattt taaaaagtat aaatgatatc tttaaatctc
     5341 atagaataac aatgtcaaaa agttaaagtg ttgatatata aaagtgatga ttttatatac
     5401 tttaaatggg acatgtaaat ctctgttgta gtacttagca atattaccga gtttaattat
     5461 taagtagtaa
//
LOCUS       DANJFZ010000146         5443 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.147, whole genome shotgun sequence.
ACCESSION   DANJFZ010000146 DANJFZ010000000
VERSION     DANJFZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.147"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(239..682)
                     /locus_tag="PWN45_002496"
     CDS             complement(239..682)
                     /locus_tag="PWN45_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624235.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(847..1224)
                     /locus_tag="PWN45_002497"
     CDS             complement(847..1224)
                     /locus_tag="PWN45_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624236.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(1542..2132)
                     /locus_tag="PWN45_002498"
     CDS             complement(1542..2132)
                     /locus_tag="PWN45_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL0624237.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(2129..2341)
                     /locus_tag="PWN45_002499"
     CDS             complement(2129..2341)
                     /locus_tag="PWN45_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDL0624238.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            2402..2948
                     /locus_tag="PWN45_002500"
                     /pseudo
     CDS             2402..2948
                     /locus_tag="PWN45_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3043..4659)
                     /gene="groL"
                     /locus_tag="PWN45_002501"
     CDS             complement(3043..4659)
                     /gene="groL"
                     /locus_tag="PWN45_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL0624239.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(4735..5019)
                     /gene="groES"
                     /locus_tag="PWN45_002502"
     CDS             complement(4735..5019)
                     /gene="groES"
                     /locus_tag="PWN45_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL0624240.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5194..>5443
                     /locus_tag="PWN45_002503"
     CDS             5194..>5443
                     /locus_tag="PWN45_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0624241.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIP"
BASE COUNT         1756 a         1075 c          661 g         1951 t
ORIGIN      
        1 tttttagaca acgctattat ttttagtttt tcaattctat tatgtcataa ttatgtcact
       61 caaaaactgt tttccaacat tgatttattg ttggaaaatc tcgaaaatct agtctatttc
      121 tccaacaatc gaagaattat gccctttttt ctccctttaa ataagtcata atacgaggca
      181 tacatgcaac atttacaata aaatatgttc ctacatcgta ttatacgaat gctcacactt
      241 atagttgttt agtaaaccca gatgtattgc taacataccc attatcacta tatagtttat
      301 gtgcagatag tctttcattt ctattaccac tatttagtgt tattgcttta caattcaacc
      361 gtttagaaaa ttcttcagaa tcagctaata atctctttcc ataacctttt ttcctaaatt
      421 cagaatgtat aacaaacgca aggattctca tatactctgc atttttttcg taaaacatca
      481 ttttacacat accacttaaa ccaattattt tattttcttt tatcaaaagc agtaggaagt
      541 aatcatcatg attcgttatt ttctttagtc tcttttttaa atcattctca tttgttggat
      601 aacctagatc atcatataat ttacatagtt gatttaattt ttcaaaatca ctgttattaa
      661 acaatcttgt aattatgctc atataaccca ccctaaaaat attatgtacc tattgttaca
      721 tacaatagat acgcaaaatc atttctcact gcaaatcagg acgtttctca gcgtaaacaa
      781 atcatttcta ttcttttgaa aaatatctgc atatcttcac agtaatttta taacagatta
      841 ataaaattaa aaaaacataa caacaagtat tataataaat attataatag ctatcgtatt
      901 aaatagttta gaatatgcaa actgaaaaat aaaaatcaca attaaaatac taagtaatgt
      961 aaatggtaat agcatcatca gatctttaac attatttctt tgttcttgct cccaatcgct
     1021 aatagcccta aaactacttt ttttatctat aggcgatgta aaaataccat atattgaagg
     1081 tgctatacct cctaaaatag cagttcccaa agttgtcatt actgaaatta ctgcgaaagt
     1141 atcatccgaa agcaataaat tcaaactaat gcattgttta ttacccatcg aatttattga
     1201 ccaaatagct agagaaataa acaacccaaa atttaaaata aatgatatag taatagcaat
     1261 tgtttacaaa acacgaaatt tttcattttt atttatatta tccatttttc tccctttttc
     1321 ttaaatcatt ttattatata ttacaataat caatctgaaa tgttgatgta atttgaaaaa
     1381 aatatcatac ttttactcct gaaaacctcc ctaaatcatc aatatgaaaa tcagtattag
     1441 gatattgcgt ttacaactct tttaaatctc actcattctt ctcatcgtct taaccgatac
     1501 tatcaataaa tactggcaac accataaaat atatacccaa tttataaaaa cctaattgac
     1561 tcataatctt ctaacggtat atcatccaca atcacagtat gattaggatt atcgttagat
     1621 acatctttca ctgccttatc taacccctca tcacctccgt cccattcacc aatattaatg
     1681 aatataggta cattaccgtt tatatcatgc ttatctgtaa ataatttatg atatttaccc
     1741 aacatatcac gagcttttaa acgatcacta ggttttattg gtacctctat cagttcaaca
     1801 tgttcattat agactaattg tactttgcca ctttgtggat tttctttata ttctccacgc
     1861 ttgactacaa cttctttcgt ttctgtttca tcaccgactg ccgcattcgt tagcacatgt
     1921 aataactctt ttgcggttaa tacattctca tctataatct tatctttttg ttcttgtata
     1981 tattgcttga tgtgcggctt tttcaataac ctacacgctg tcacatgtgc gctatttgcg
     2041 ttatatcctg cttttatggc actttgtgtt acattcagtg ttctaatata ctcattcaca
     2101 aaacgtgctt gctttgcagt taactcactc attttatcac ccccacaatt ttatctaata
     2161 tggtttcata ccataatatt acagattgtt ctgaacaatc taaggcacta ctaatatctt
     2221 gataactaag tccttgtata agggagtcaa aaatataaaa ctctttatcg gtcgctaatc
     2281 tgtcaacaat catttctatg tgattcttta taatatgatc attgacatta tcgtctgtca
     2341 tcaattcgtc agaatcttca tcacctatta aaaagaaatc atcagtattt atttcatcat
     2401 cgccccgtta actagctttg aagtctttag cacacttgca tataccggct gtcgtgctgg
     2461 cgagatacta gcattgaagt ggtctgatat tgattttgaa aacaacacga ttagtattac
     2521 taaaacatat tacaatccaa ataataacaa gaagaaatat cagatacttc cccctaaaac
     2581 tgaaagttct atcggtaaga tttccgttga cccaaatgta ataaaggtgt tgcgtgatta
     2641 taagataaac gttcaaaata attggaaaaa cgaattatat aatgataacc attgtaatga
     2701 aaaaactatc aatgtggata caaaccatta tgtcacgtac cagcatactc aaaaatatct
     2761 ctacgcactc attcagatac acacattgcg cattgctcat agaatcaggt gtacacatta
     2821 aagaaataca agaacgatta cggcataaag atatcaatac cactatgaat atctatgcta
     2881 aaatcacaaa ttcatacaaa aaagacgcct cccataagtt tagtcaccaa atggaagacg
     2941 tctcgtaatt aaaatatatt tgcgtgcatt ctaatttata cttagaatga atcatactcg
     3001 tgcataatgt aattttctag ttagtcaaaa ctataaacag ttttacatca ttcctggcat
     3061 gccacccatg ttaggttggt cattattttt ttctggaatt gatgctacaa ccgcttcagt
     3121 cgttaagaac attgctgcaa cacttgcagc atgttgtaat gctgagcgtg ttactttagt
     3181 tggatcaacg ataccttctt ctaacatatt aacccactcg tttgtagcag cgttaaaacc
     3241 aacacccggc tctgcgtttt tcaaacgttc tacaataaca gaaccttcta atcctgcatt
     3301 ttcagcaatt tgacgaactg gtgcagttaa tgctttaagt acaatattta cacctgtttc
     3361 aatgtcacct tcagcttcaa tttcacttac tttttggtaa acatttacta atgcagtacc
     3421 accacctgca acaatacctt cttcaactgc tgcacgtgta gaatttaatg catcttcaat
     3481 acgtaattta cgttctttaa gctctgtttc acttgctgca cctactttga taactgcaac
     3541 accacctgct aatttagcta agcgctcttg taatttttca cgatcaaagt cagattcagt
     3601 ttcttcaatt tgagatttca attggctaac acgtgcatca atgctgtttt cgtcaccgtc
     3661 accatcaaca acagtggtat tatctttagt tacttctact ttacttgcag tacctaacat
     3721 atcaattgat gcatctttta aatctaagcc taaatcatca gtaatcactt gcgcaccagt
     3781 taaaatagct aaatcttcaa gcatcgcttt tctacgatca ccaaaaccag gtgcttttac
     3841 tgcaacagct gtaaatgtgc cacgcatacg gtttagcacg atatttgtta atgcatcgcc
     3901 ttcaacttca tcagctacaa ttaagattgg acgattagat tgaaccactt gttctaataa
     3961 aggtaagata tcttggaaag acgagatttt cttatctgtt actaaaatgt atgggcgttc
     4021 taattcagca accattttat ctgaatcagt aaccatatac ggtgattgat aaccacgatc
     4081 aaattgcata ccttcaacca cttctagttc agtgtttagt ccatttgatt cttcaattgt
     4141 aatgacacca tcgttaccta ctttttccat agcttcagaa atataacgtc caatttcttc
     4201 atctgctgct gaaatcgcac ctacttgcgc aatttcattt ttattttcaa ctttttgaga
     4261 attttcatgt aacgcttcaa cagcaacttt aactgctttg tcgatacctt gtcgtaaacc
     4321 aactgggttc gcaccacttg taacattttt caagccttct tgaatcattg cttgagctaa
     4381 tactgttgca gttgtcgtac cgtcaccagc aatttcattt gtcttatttg cgacttcttg
     4441 aactagttta gcccccatat tttcatatgg atcttctaat tcgatttctt tagcaatcgt
     4501 cacaccatca ttcgtaatta aaggtgctgt aaactcttta tctaatacaa cattacgtcc
     4561 tttaggacca atcgttactt taactgcatt tgcaagttgg tcaacaccac gtaacattgc
     4621 ttgacgtgca tcttcagaga atttcaattg tttaaccata aatgataaac ctccatttgt
     4681 ttagttgtgt ttcattttcg ttctatttaa tttatttatg aattaagttc tgtattattc
     4741 aataactgct aaaatatctt cttcatttaa taccagatat gtttcattat ctcgtttaac
     4801 ttctgtacca gcatattgtt ggaacacgac acggtcccct tctttcactt caggagtcac
     4861 tcttgtacca tcatttaata ggcgtccagt tcctactgca acgataacgc cttcgtttga
     4921 tttttcttta gcactatcag ttaaaacaat accactttta gttgtttgtt cttgttcttt
     4981 tttctcaata atcacacgat ttccaattgg ttttagcatg attgttcctc cttaaaaaac
     5041 ctaaagttta gcacttaaca ttaaagagtg ctaacataca tttataataa tcaaatttgg
     5101 tcaaaatttc aagtcataac cttttaatta agttttgtaa cttcaagtta tttacgataa
     5161 aataaattat aaacaaatat ttggaggaaa attatgacaa gattatgggc atcattgcta
     5221 actgttatta tttatatatt gtctcaattt ttaccgcttc tcattgtaaa aaaattacca
     5281 tttgtacaat atagtggcat agaactgact aaagcagtca tttacataca acttgttcta
     5341 tttttaatcg ccgccacgac gattatttta attaatttaa aaattaaaaa tccaacaaaa
     5401 ttagaattag aagttaaaga acctaaaaaa tatatcattc cat
//
LOCUS       DANJFZ010000147         5433 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.148, whole genome shotgun sequence.
ACCESSION   DANJFZ010000147 DANJFZ010000000
VERSION     DANJFZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.148"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..313
                     /locus_tag="PWN45_002504"
     CDS             <1..313
                     /locus_tag="PWN45_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDL0624242.1"
                     /translation="IYYKEQQERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEG
                     YINENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKND
                     R"
     gene            580..999
                     /locus_tag="PWN45_002505"
     CDS             580..999
                     /locus_tag="PWN45_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDL0624243.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            1017..1436
                     /locus_tag="PWN45_002506"
     CDS             1017..1436
                     /locus_tag="PWN45_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDL0624244.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            1888..2310
                     /locus_tag="PWN45_002507"
     CDS             1888..2310
                     /locus_tag="PWN45_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL0624245.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(2422..3960)
                     /locus_tag="PWN45_002508"
     CDS             complement(2422..3960)
                     /locus_tag="PWN45_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDL0624246.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            4193..5011
                     /locus_tag="PWN45_002509"
                     /pseudo
     CDS             4193..5011
                     /locus_tag="PWN45_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            5274..>5433
                     /locus_tag="PWN45_002510"
     CDS             5274..>5433
                     /locus_tag="PWN45_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624247.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVI"
BASE COUNT         1970 a          810 c          970 g         1683 t
ORIGIN      
        1 aatatactat aaagaacaac aggagcgtat tacactctat cttaagcata atactaaaga
       61 acccaatacc atcaaaactg tacatttcac aagtttaaaa agaggaccta tgggcgatgc
      121 agtaattgaa ggttacataa atgaaaataa agaagatgat tttgttgcat atgggtctcc
      181 agaacataat tatcaatttg gtggaagttt aatcaaaagt aaaaatttaa gcacgttatt
      241 aaaaccagta catcaaacca aatcacctga tgagataaaa aaggaattag agtctaaaaa
      301 gaacgaccgc taaaactatg cggccgtttt aattttgttt tcaagttaat catacttaca
      361 tataaaaatg aaaagttgaa tttttagtac aatttccact gtatttatct gttaaccact
      421 ttatacccgc aatatttcgt tgatcttata taaacacttt tggctgcttt tccttgacct
      481 agattaaaat tatagatttt ctttgtaaga gtgtgttttg tagtatcata aatatgtaaa
      541 ctgtataaaa ttccaccaca ttttaggagc tggtgataga tgtcgaaaaa gaaaatttta
      601 atattaacta gtattatgtt aatcatatta attagtgttg caggtatcta tttaaaagtg
      661 aaatatgacg aaaaagaaaa acaaaaagca atatactata aagaacaaca agcgcgcatt
      721 acactttatc tcaagcataa cactaaagaa ccgaatacaa tcaaatctgt acatttcaca
      781 aactcggaaa caagtcctat gggaagtgct gtgattgaag gttacataaa tgaaaacaaa
      841 aaagataatt ttactgctta tgctacacca gaacataatt atcaatttgg tggtgctatg
      901 atagaaagtg aaaaattaag cgagttacta aagccagcca atcagttaaa atcaccagat
      961 gatataaaaa aagaactaaa taaaaagaag agtcactaaa gttaggagtt actttaatgt
     1021 ccaaaaaaca tgtttttata attattggtg tcatattgtg tatatgtaca gtttctacgg
     1081 tcatgcattt taaaatgaaa tatgatgaaa aagaaaaaca aaaagcgatt tactacaaag
     1141 aacaacaaga acgtattaca ctctatctta agcataatac taaagaaacg aacacgatta
     1201 aatctgtaca tttcacaaac ttggaaacaa gtcctatggg aagtgctgtg attgaaggat
     1261 acatcaatga aaataaagaa gatgatttta ctgcttatgc atcgcctgaa cataattatc
     1321 aatttggtgg cgctatgata aaaagtgaag gagtagataa attattaaaa ccagcacatg
     1381 aaagaaaatc accagaaaaa atcaaagaag aattagataa aaaagaaggc cactagggtc
     1441 ttctttattt ttgatttaat cttccaataa tctatgtcat tgctatcgaa ggtgtttcgc
     1501 aattaatata aatcacttca tcatcaccaa tacttcccca gttttgtaca gtacattaac
     1561 acaaacgaac cacgttaatt taaatggaat aatagtttgg ccattataag aacaatatat
     1621 atcgaataac aatgtcgatg acgtgattta ttgacattat caaattttct caaatgtgtg
     1681 tgatttttta taaaaaaggg tagagcaagg tataatatcc tttcaatctg ttttcatatt
     1741 ttatatattt tttaaatata agtgctaaat gttttaacta aagcatagat tgacaagatg
     1801 ttatacagaa tttcaaattc tatccaatat tgttcgaagt gtagtatcac tggattggta
     1861 ttaaacaatg taaaggagag attgcaaatg ccgtataatt acaagaaaca aaatggagag
     1921 ttaatgtctg taatgagcca aggtgaaaag tttattcatc aatcacccgt taatgatgaa
     1981 cttagtgcat tgattaagtt attaatttct aaaattaacg gttgtcatta ttgtgttgat
     2041 atccataaaa aagaattaaa ggaattgggt gtaacacaaa tgaaaattga tgaagtcttg
     2101 agttttagac atttagattt atttactgat caagaaaaag tgacgcttga atttgcagaa
     2161 atgttaaatt caatcaaaga ctttaagaag tttgaaatta ttgaccggct aaaatcattt
     2221 tatgatgaag aacaaattat tgatcttgtc tttgttgtaa accaaattaa cggttggaac
     2281 agattaaata ttattagtga tagactataa ttgttcatat aaatgcagag tttcatctcg
     2341 aacgctatat cataacaaat catgccacta tacaggtcaa atcttgtata gtggcatttt
     2401 aatttatccc tttgaatact gttatttaac gaatatcggt ccacctggtc caacagggat
     2461 acctaatagg aaccaaatga tgacaaatac tgtccataca atacttagcg cgattgaata
     2521 cggcattaaa ctagaaagta aggctccgag tttcatgcgt ttatcgtatt tttgtgcata
     2581 agttaataat aaaggtaagt acggcatcat cggtgtaatt ggattggtaa ttgaatcgcc
     2641 tacacggtaa atgacttgtg tgaatgcggg atgaaagccg ataaggatta acattggtac
     2701 gaatatcggt cctaaaatac cccatttagc cgatgcgctt ccgattaaca tgttgaccat
     2761 tgcactcagt acaataatac ctagtatcaa tacaataccg ttttgatgtt ctaataattt
     2821 ggcaccttta acagcagcga taattcctaa attactccac tttaaatacg caagtagctg
     2881 tgctgcaaaa aacacaataa cgataaatgt tcccattgat cctacagcat cgccgaacat
     2941 tttacctaag tcttttgtat ttttaatttc tttgcttaaa atcccataaa ctaatccagg
     3001 tactaaaaat acgacaagaa taattaatcc gacaccgtta attaatggcg catcgtctag
     3061 taagctgcct gttttagcat ttcttaaaaa gctatgttca ggaatggctg taataattaa
     3121 taaaataatt gtgactatga aactgatatt tgcccatttt aaagcatgtg cttctttgtc
     3181 agtaatatgt gaagatgttt cttcatggtc atcatgcatt aaactgtcat catattttcc
     3241 taatctagga ataatgagct tagttgtaac tagcaatatc gtaggaagta atacaacgac
     3301 actcgctgcg ataaagtacc agttcatagc aacgtttgtt ttaatagaat ctgaaacgat
     3361 acgtgttgcc ggttctgtaa atgaatagac caaagcatct tgcataccaa caactatatt
     3421 tgctgcaaat cctccaacag cggaagcata tgccatcgtt agtccagcga tagggtgata
     3481 gccaatttta ataaaaagca ttgctgcaag cggcggcaag ataattgtcg cagcatcgcc
     3541 ggctgtacta cctaaaatac caattaatat aatagtcggt aaaattaaga agcgtggtgc
     3601 gcgattcaca acagaaatca ttaacttatc gaagtatcct gttttctctg caacaccaat
     3661 accaatcatc actgctagta ctaagcctaa tgctgggaac tctgagaaat ttttaatcgt
     3721 atcattcatt atcatcgtaa atccatcatg gctaattata tttttaatat aaatggtttg
     3781 atgcgtacct ggatgcttaa cagatacatt aaataatgag ataacccatg tcataatggc
     3841 taagcccaca cacattaaaa agaataagac gctaggatct ggcaatttat ttccgatttt
     3901 ttcaacacta ttcaagaaac gattgacgat agaccccttt tgttgatgtt ttgatgtcat
     3961 caattattcc cccctttgtt aaatatttaa agtgtaacaa aaaatactct caaaagtaac
     4021 aattttcaga aaataaaaaa actaatattg ttaaatattt tgagttattc aatagaaagt
     4081 gtatagcaga gtagttaaga ctgcctgaag acttatctat taggtttatg aagcatcgaa
     4141 cagtggaaaa taaggactgt aagtttaaga tatgttgtat aggagtgact gaatgaaacg
     4201 tttggaaaat aaagtagcag tcgtaacagg agcaagtaca ggtatcggtc aagcttctgc
     4261 aatcgcttta gctcaataag gtgcgtatgt attggcggta gacatagctg aagcggtatc
     4321 ggagactgtc gataaaatta aaagtaatgg tgacaatgcg aaggcgtata atgtggataa
     4381 tgcaagcgaa caacaagtgg tagactttgt gtctgacata aaggaacagt ttggaagaat
     4441 cgatgtgttg tttaataatg ccggtgtgga taatgcggct gatagaattc atgagtatcc
     4501 aatagatgtg tatgacaaga ttatgaatgt agatatgcgt gggacatttt taatgacgaa
     4561 aatgatgtta cctttaatga tgaatcaagg tggctctatt gttaatacgt cttcattttc
     4621 cggacaagca gcagacttgt atcgctctgg atataatgct gcgaaaggtg cagtgattaa
     4681 ttttacaaaa tcaatcgcaa ttgagtatgg ccgtgatagc attcgagcca atgcgattgc
     4741 accaggtaca attgaaaccc cgttagtaga taaactgaca ggtacgagtg aggatgatgc
     4801 aggtaaaaca tttagagaaa atcaaaaatg gatgactccg ctgggacgtt taggtaaacc
     4861 agaagaagtt gctaaattag tagtcttctt agcatctgac gacagttcat tcataactgg
     4921 agagacgatt cgaattgatg gtggtgtgat ggcttacaca tggcctggcg agatgttaag
     4981 tgatgattcg tggaagcgga cattggaata attatttaaa atcgatatcc agtggaaaag
     5041 aatttggcat gaagtaggca atgtatgcat attgtcgcaa tgaaagacat ttaaagcgga
     5101 gattaattaa tctttgaaaa tcacatattg ttcatttgaa gtgtattgct taaagcttgt
     5161 tttaacgtat tgtaggtcgt gcatctaatt tatcagaatc tcgagttcaa acttttggag
     5221 tatctttaaa ataagctgta ttttatctat tctaataaat taaggagaat tttatgttaa
     5281 aagaaaaaga aagttttaga ttgctatatc aagctataag agagattgca gataaaattg
     5341 gagataatca gttagaaact aattccgtta gtttattatt attggacttt gattttgaac
     5401 atgaagtatt tgatgaattg tatcttgtga ttt
//
LOCUS       DANJFZ010000148         5340 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.149, whole genome shotgun sequence.
ACCESSION   DANJFZ010000148 DANJFZ010000000
VERSION     DANJFZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.149"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(636..1082)
                     /gene="perR"
                     /locus_tag="PWN45_002511"
     CDS             complement(636..1082)
                     /gene="perR"
                     /locus_tag="PWN45_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDL0624248.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1179..2129)
                     /locus_tag="PWN45_002512"
     CDS             complement(1179..2129)
                     /locus_tag="PWN45_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL0624249.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(2135..2590)
                     /gene="bcp"
                     /locus_tag="PWN45_002513"
     CDS             complement(2135..2590)
                     /gene="bcp"
                     /locus_tag="PWN45_002513"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL0624250.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2671..3960
                     /locus_tag="PWN45_002514"
     CDS             2671..3960
                     /locus_tag="PWN45_002514"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL0624251.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4253..>5340
                     /locus_tag="PWN45_002515"
     CDS             4253..>5340
                     /locus_tag="PWN45_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0624252.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDF"
BASE COUNT         1753 a          844 c          775 g         1968 t
ORIGIN      
        1 tttgtcactt acaagattta attataactc gtaagttttt gtaagtcaag aattattttt
       61 aattatttta tttctaaaaa ataaatttcc taactcaatt aattcaacaa atattatttt
      121 attatgtatt tgttcgaatt tcaatagtca aatttacact ttattaatat tttaaatcaa
      181 caacttaaaa catttgtttt ttaaatatta gtgccgtttg acgactttta tataatatca
      241 agttattcta gtagagtcaa cataaaacaa cacttatttt tgtttaaaaa ttactgttga
      301 agggattatt ttctattcgt aagtactata tgtatttaaa gtgtaattcc ttatgctttt
      361 tacttcagtt ttacaaacaa acttatactc actttatgga tagttattta gtagaccttc
      421 gtttgaatat ttttatgttt atttataaag gatactagtt attagttaat accacttccg
      481 tagttagaat attcttttct tcaatttaaa tatttttcag aataagcata aaaaaaagtt
      541 aaacaacttt catgtcgttt aacttcttaa atattacgct tacttatatg aagtaagcta
      601 acgctttttt aatttgtcat actaccaaag ttaaattatt tatcttggca ttctttacaa
      661 actccataaa tttccattcg atgatgtgtt acgtcaaagt cagtcatatg ctgagctaat
      721 ctttcaattt catttaactg tggatattga aaatcaacaa tcttaccaca ttgttcacat
      781 ataatatgat aatgattatg tgtattaaag tcgaatcgac ttgatgagtc tccatatgtt
      841 aattctttta caattccaat atctttaaac actcttaagt tattatatat tgtcgcaaca
      901 cttatatttg gaaaatcagg tgaaagtgct tgataaattt catcagctgt tggatgagta
      961 tgtgaagaaa ttaaataacg taatattgct tgtctttgag gtgtaattct tacgcctgct
     1021 tgtcgcaatg atgcaattga ttcttctagt tcatgttcaa ttgattctat ttcaacactc
     1081 atctatatca ccatctttct tatataataa ttattattac ttactataaa tataactgta
     1141 gtattgcgcg cgtgtcaata tttacatgat gatttcattt aatagccttt tttagcatca
     1201 acttcattct caattagacc attcttattg agaaaattaa ctagattgtt tttaaaaata
     1261 tctaataagt catactttgc ttcataatca ttaccagtta tatgcgctgt tatagttaca
     1321 ttatccaatt catataattc atgattaggt ttcaaaggtt cattttcaaa cacatctaaa
     1381 tatgcatgtc gaataacttt actttttaat acttctatta agagcgcttc tttaactatg
     1441 ctacctcgtc ctatatttat aaaaagtgct tcatctttca ttaattcaaa atgttttttc
     1501 tttagtaaat gaatcgtttc ttgcgtttct ggtaaagcat ttataataat gtcagcattt
     1561 ggtaatgtgc tttcaagtga ttcgatagta tatatctcgt caaactcatc tttgttctga
     1621 cctgacttgc tcagaccaat taaattcata ttaaaagcct ttgctaactt cgcagttcta
     1681 gtagcaattg cacctgtacc taaaaataaa actgtttgtc ctgataggcg tttaccagtt
     1741 attttcgaat catatatatg ttgtcgttgg ttatcatatg atagtttcat ctttttataa
     1801 tcatctaaaa tgaaagctaa aatgtattca gataattgtt tagcttgaac accttttcca
     1861 ttagttaaaa gtatgccgtg atctgcaata taatctaacg gcaatgtatt tacacccgtt
     1921 gcaaaccatg caatccattt taaattcggg caacgtcgta aaaatgcctc attgataccg
     1981 ccatcataac caactaatat atctaaatca gccaagtcgc tctcaggtat ttctgatgct
     2041 tttttataaa atttaaaatc taaatctgaa aagcgttgtt gtaattcagt ttcaacttca
     2101 cgcatacgat ttaacccaac aactttcata tatgtcaccc caaaatgttt ttaagttctt
     2161 ctatttgtgt tttaacctta accttctcga taacatctaa tactttacct tgttcatcta
     2221 ttataaaagt cgttcttaca atgcccatac tttctttgcc aaatgatttt tttaactgat
     2281 atacgccagt ttctttagct aatttaaaat cttcatctac taataaatcg aaattcaatc
     2341 cgtgtttctc aataaaattt tggtgttttt tctttgaatc accgcttata ccatatactg
     2401 caacatctaa atcattgaac atttctaaat tgtctctaaa gtcacaagct tctgtggtac
     2461 aagtaggtgt attatctcta ggataaaaat atataatcgc ctttttacct tttaatgtat
     2521 catttgtaat gacagttccg tcttgatttt ctaatttaaa tattggaaat tgttctcctt
     2581 tttgcaacat ataatcacct ttttcttcat tataataatt ttatgatacg atattagatg
     2641 aaaatattaa attaaaagag gttgatataa atgaatttta gtgaaagtga acgtttacaa
     2701 caactttcaa acgaatatat tctaggcggt gtcaattccc cttctcgttc ttataaagct
     2761 gtaggaggcg gtgcacctgt tgttatgaaa gaaggacacg gtgcatattt atatgatgtc
     2821 gatggcaata aatttattga ttaccttcaa gcatacggtc caattattac ggggcatgca
     2881 catcctcata ttactaaagc aattcaagaa caagctgcta aaggtgtttt atttggtaca
     2941 ccgactgaat tagaaattga attcagcaaa aaattacgtg atgcaattcc atctcttgag
     3001 aaaattcgct ttgtaaattc tggaacagaa gcagtcatga caacaattcg tgttgcacgt
     3061 gcatatacta aaagaaataa aattataaaa tttgctggat cttatcatgg ccattctgat
     3121 ttagtattgg ttgcagcagg tagcggccca tctcagctcg gttctccaga ctcagctggt
     3181 gttccagaaa gcgtcgcacg tgaagtcatt actgtacctt tcaatgatat taacgcctat
     3241 aaagaagcaa ttgaattttg gggtgatgaa attgccgcag tattagtaga accaattgtt
     3301 ggtaactttg gaatggtaat gcctcaacct ggatttttag aagaggttaa tgaaatttca
     3361 cataacaatg ggaccctagt gatttatgat gaagtaatta ctgcattccg tttccattac
     3421 ggtgccgctc aagatttatt aggtgttatc cctgatttaa ctgcatttgg taaaattgtt
     3481 ggcggtggtt taccaattgg aggctatggt ggacgtcaag atattatgga acaagtagca
     3541 cctctaggac ctgcatatca agctggtaca atggctggta acccgttatc tatgaaagca
     3601 ggtattgcat tactcgaagt actagaacaa gacggtgttt atgaaaaatt agacagctta
     3661 ggccaacaac tagaagaagg tttacttaaa ttaatcgaaa aacataatat cacagctaca
     3721 attaatcgta tttatggatc tttaacattg tactttacag atgaaaaagt cacacattat
     3781 gatcaagttg aacattctga cggcgaagcg ttcggtaaat ttttcaaatt aatgttaaat
     3841 caaggtatca atttagcacc ttctaagttt gaagcttggt tcttaacaac tgaacataca
     3901 gaagaagata ttaaacaaac tttaaaagct gcagactatg cttttagtca aatgaaataa
     3961 cttttaagga agtatatttt taattgaatt aaaataaagc aaatgctcac atagtagatt
     4021 cattttatat cgttaactat taacgtttaa cttgatatgt cactatgtga gtttttattt
     4081 tacaaattta tactatttta atctctaact aaagttttac atcgctatca gtattatgta
     4141 tgatttattt taacaactat aaataagctt gaattttgta actagtaagt gtataacagt
     4201 aatgaatacg aatattagat attcaataaa cttaaaaggg gcgtcattat ttttgagact
     4261 aggagctcgg atttttaaaa ctggtatagc cattatttta gctatgtcta tcgcttcttt
     4321 actaccggat gatgttggtc tgaaagcctt agctggtgtc agtgctgttg ttgcaatgca
     4381 acctagtatt tatcgttcat ttaaaacagt ttctgatcaa gcattgggta acattatagg
     4441 tgccatacta tctgttacaa tggtaacaat ttttagtgat aattttatca ttatgggtgt
     4501 taccgtcata gtacttattg ctattttatt taaatttaat ttagcacatg ttgctacttt
     4561 ggcgagtgta acagcattaa tcattatggg acaacatacc ggttctttct atattaccgc
     4621 cttttacagg tttgtacttg taatgattgg tgtcataagt tcatctctag ttaactttgt
     4681 gtttctacca cctaaatttg aaacaaaaat ttactataat tcattaaata tatcctcaga
     4741 tatttttatg tggtttaaat tagtattaaa tgatacgacg gagtttaata atattaaaca
     4801 agatagtcat aatttaaaac aacgtgtcga aaagttagag aaaatttatg attactatag
     4861 cgaagaacgt cctattacaa aaaaacatat tcaccaacag aatagaaaga aaatactttt
     4921 cagagaagta gttcagacga ctagacaagc ttatgaagta ttaaacaaat tgtcgcgtta
     4981 tcaaaacgac ttatatttac ttaataacaa tttcctatta caaatcaaat tagatttaga
     5041 ttcattaact gcttttcacg agcaaatttt agctagcctt tctaaaaaag cacgttataa
     5101 tgtgacacat gttgattatg agttagataa tccgcagaaa aaagatttat tgtccacatt
     5161 tcagcatgag ttaataaatc atccttatca aacggaatat tcgtttgcca atgttatgca
     5221 aattgttgca gccatcgaag agtatcgaca ccatttagag catttagacc gcataagaat
     5281 tagctttttc acatatcata gatctgatgc agacattgaa attgttgaag aagactttga
//
LOCUS       DANJFZ010000149         5310 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.150, whole genome shotgun sequence.
ACCESSION   DANJFZ010000149 DANJFZ010000000
VERSION     DANJFZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.150"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..849)
                     /locus_tag="PWN45_002516"
     CDS             complement(<1..849)
                     /locus_tag="PWN45_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDL0624253.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHS"
     gene            956..1801
                     /gene="blaZ"
                     /locus_tag="PWN45_002517"
     CDS             956..1801
                     /gene="blaZ"
                     /locus_tag="PWN45_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDL0624254.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(2327..2746)
                     /locus_tag="PWN45_002518"
     CDS             complement(2327..2746)
                     /locus_tag="PWN45_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0624255.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            2870..3841
                     /locus_tag="PWN45_002519"
     CDS             2870..3841
                     /locus_tag="PWN45_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0624256.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(4013..4105)
                     /locus_tag="PWN45_002520"
     CDS             complement(4013..4105)
                     /locus_tag="PWN45_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0624257.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(4262..4609)
                     /locus_tag="PWN45_002521"
     CDS             complement(4262..4609)
                     /locus_tag="PWN45_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624258.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(4628..5245)
                     /gene="cadD"
                     /locus_tag="PWN45_002522"
     CDS             complement(4628..5245)
                     /gene="cadD"
                     /locus_tag="PWN45_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDL0624259.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2002 a          719 c          731 g         1858 t
ORIGIN      
        1 tgaatggctt aatatatttt tgttggaaaa tggggttttt aatacggagt ccataataga
       61 ttccgcataa gttttaaatt catttttgtt aatattatta aggacaaatc tatcggcttc
      121 tacttcattg tcatgaatta tctttctttt tacaatatgt actaatggat tgtaactcat
      181 aataatagag aagatattaa agataattaa atgtaaagta tctctatttt tagcatgagc
      241 atattcatgc agtattatat attttagtct tttatcaatt acacttttaa aatatgaact
      301 aggaatcaaa ataatatatt tcccatacca aaaagttatt ggagattgaa tagcctctgc
      361 ttttcgaatc acaatatttt ttttatattg atggttgaaa agtatcgtat ctattttatt
      421 tttttcattt tcgtttagat aaagtgactg tttctttaaa tatttaagat ataataaggc
      481 tttcaaaaat ttaaaactta aaataataac taaaactatc caaataactg tgcagatatt
      541 atcaattgaa tcccaattaa acttatggat atctgttgtg aactcttgaa taggtttggt
      601 ggtatttatg ttatggttca aatcgtgtga cttactttca actgtgggcg cttgattatt
      661 cacattatta aattttataa aagagaattt aattgggata aaaggaatta atccagcaag
      721 catagttaga taccaaactt tataatttaa actataatta aaatagcgtt ttaatatgta
      781 cctaaaaaat actaacaata gaaatataaa acaaaagctt actacgctca ttattagtaa
      841 tttagccatt ttgacacctt ctttcaaata tttataataa acaattgaca tcaatattac
      901 aattgtaata ttattgattt ataaaaatta caactgtaat atcggagggt ttattttgaa
      961 aaagttaata tttttaattg taattgcttt agttttaagt gcatgtaatt caaacagttc
     1021 acatgccaaa gagttaaatg atttagaaaa aaaatataat gctcatattg gtgtttatgc
     1081 tttaaatact aaaagtggta aggaagtaaa atttaattca gataagagat ttgcctatgc
     1141 ttcgacttca aaagcgataa atagtgctat tttgttagaa caagtacctt ataataagtt
     1201 aaataaaaaa gtacatatta acaaagatga tatagttgct tattctccta ttttagaaaa
     1261 atatgtagga aaagatatca ctttaaaaga acttattgag gcttcaatga catatagtga
     1321 taatacagca aacaataaaa ttataaaaga aatcggtgga atcaaaaaag ttaaacaacg
     1381 tctaaaagaa ctaggagata aagtaacaaa tccagttaga tatgagatag aattaaatta
     1441 ctattcacca aagagcaaaa aagatacttc aacgcctgct gctttcggca agactttaaa
     1501 taaacttatc gcaaatggaa aattaagcaa aaaaaataaa aatttcttac ttgatttaat
     1561 gtttaataat aaaaacggag acactttaat taaagatggt gttccaaaag actataaggt
     1621 tgctgataaa agtggtcaag caataacata tgcttctaga aatgatgttg cttttgttta
     1681 tcctaagggc caatctgaac ctattgtttt agtcattttt acgaataaag acaataaaag
     1741 tgataagcca aatgataagt tgataagtga aaccgccaag agtgtaatga aggaatttta
     1801 atattctaaa tgcataataa atactgataa catcttatag tttgtattat attttgtatt
     1861 atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta ttttcgagat
     1921 ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa atcataaata
     1981 atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta tcttatttaa
     2041 agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca agcaaagtga
     2101 caatagcatt ttacaagtat tttttattgt tcgattaggg taaacctaaa taatacataa
     2161 attatattac aatatgttta ttatagaggc taaagtatct tagaaatttg gatagtaggt
     2221 ttttcgggaa taaaagtata gttggcttta atatataatt aagctaagct atcatttata
     2281 tttactcgat tataattcgc acttcaactt ttttataacg taaaaattaa aacgtagttt
     2341 tatttatctt tagtaatatc tttttaaaca ctacagtttc ttcattagtt aaattagcga
     2401 agtaatgatt attaagttga taggctttct tttctaatgt tggccataag tcttttgctt
     2461 tatttgtact gtttattagc acagttcttc tattttcatt atcagtttga cgtgttatta
     2521 gatccttttt ggacatctta tctaatattc cagatattgt tgaaggttct aaatttaaag
     2581 tattaccaat cgctttaggt gtattcaaat tactattaat caagcaattc aaaacaccat
     2641 attctgctgg agtaatatta tagggactta ataaactttt aaaatgttta aaaactctat
     2701 tctgagaagt actcaacaaa taatttatac aattatctaa ttccacaact aaaacctctc
     2761 ttaaatcatt agtattgact cgcttatcat atcatgataa gttttgtagg aaaatagttt
     2821 tgcaacaaaa acatacttct acaaaacaat tgtgaaagaa ggctaataaa tgttcaatgc
     2881 ggtagtagtg agaaaaagtg catcaaatgt tagttataaa gtagaaaaag ttgatgaatc
     2941 tcatttatcg aagggtgatg tattagtaaa agtggtatac tcttcaataa attataagga
     3001 tatgctagct ttacaatata aaggcggtgt aattagagat tatcctatga ttcctggtat
     3061 agattttgca ggtattgttg aatcaagttc gaatgataag tttaaagaag gcgataatgt
     3121 attagttact ggttatgatg ttggcgtgac acatacaggt ggattttcag aatatgcaca
     3181 agtgcctgga gagtggattg ttcctattcc ccaagaaatg tctattaaag agtctatggt
     3241 atatggcaca gccggtttta cggcagcatt atcaattttt gaactagaaa aatatggaat
     3301 gaatatcaaa tcacaacctg aaattttagt gtctggggct acaggtggtg tgggcagtgt
     3361 tgctatacaa attttacata aaattggata tgaaaatatc actgctatcg taagaaaaga
     3421 aaagcaaata gaaatcgcaa aaaaattagg tgcaaaacaa gtaataataa ttgaagatga
     3481 taataaaccg cttaaaaaag gtatttttga ttatttctta gatacagtgg ggagtaatgt
     3541 aacattatac ggtttaaaac gtctaaatta tcaaggtgtt gctactgtat gtggaaatgt
     3601 agcaggaaat tcacttaacg ctaatatatt accgtttatt cttagaggta ttaaattaat
     3661 tggcattgat tctgtatatg ttgatcataa tatccgtgaa gatatatgga gtaagttagc
     3721 aaatgaatgg aatataggaa attcacttct atacaatgaa attggatttg aagaattcta
     3781 taaaacgata gatcagcttt taaaaggaca acatttagga agaactattt taaaagtata
     3841 agtgtgagta ttcattaata catttactat taattcaatc attttgcatt tctctactaa
     3901 ccagtgttat aatttatata acgaaaatga ttaacaatta atgcaccaat ccccacacta
     3961 ttgcagtagt gtggggattt tttcgatgct ggcttataaa gtcgcctatt aattatttgt
     4021 tgcgtttact aagccaataa gtataatacg caacagcaca gccactgatg actggtgcta
     4081 tgatgtgaac gaaaatgatt agcaaagtaa tgcacctcct ctcttcgtca attgacgcct
     4141 gagagatagg cgactctatt attataccat ctgtgaatat acaagcatat ctacttccga
     4201 taagtcgtct tatgttatat aactttgttt tttatagtat caagacaaga agaaactcgt
     4261 ttcaaaaacc ctcatgattt ttagagaaaa actctcttat ttcatcgtct ttaataaaat
     4321 aatatgccat ctttccatct ttataaaaat caagtacctc atttttatat aaaagcctta
     4381 aatgatgtga agttgaagca acactcattt tcaatatcaa agaaatatca caaacacaca
     4441 actcatcttc tttaatcaaa gataatatga ttttcaattt cttctcatca caaatttttt
     4501 ctaaaatgtt aagtaatttc ttagatttat catcttctaa aaaacttaaa gcattgttaa
     4561 ctttatcctc atgtacacag atcacatcac aagtattttc ataactcata taaaatcaca
     4621 ccctaaatta aaatataaat cctaaaattg tttgaatagt gtcattttca ataataataa
     4681 ataaacctaa agctatataa ataatagcca taatccaacg actaaatttc tcaacaattt
     4741 ctccaattcc tggaatatta gctaattttt gtgcagtaaa caccaagaag aaaattaaaa
     4801 ttaaaaagac aaacagagta agtaataaat tagtaacact taatgtcaca aaatacggaa
     4861 caaataaacc aatattatcg gcgccacaac ttgctatcgt aacaattgca accgtaccaa
     4921 ctaatttaga caatcctttt tcattcaatt cttttttagc tctcttttct ccttcacaat
     4981 catcataaat agccacttta attccaagat aaattggtat taaacccaat aaacctaata
     5041 tccacttttc tggaacataa tttaatacaa aagctaaaaa caaactaact aatattaaaa
     5101 taatagaccc taaatattga ccaacataaa tatctctata ttcttttcta gtctttgctc
     5161 tagcaaaaaa tattaataaa attactaata aatcaactgc tgtagcaata taaagaacag
     5221 cagcagcaac aaccgtttga atcataatac acctcattca aaaatatttt tgaatgtatt
     5281 ataacttata tttttaaata atcataggtg
//
LOCUS       DANJFZ010000150         5234 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.151, whole genome shotgun sequence.
ACCESSION   DANJFZ010000150 DANJFZ010000000
VERSION     DANJFZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.151"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            57..617
                     /locus_tag="PWN45_002523"
     CDS             57..617
                     /locus_tag="PWN45_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0624260.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            670..1599
                     /locus_tag="PWN45_002524"
     CDS             670..1599
                     /locus_tag="PWN45_002524"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL0624261.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1794..1967)
                     /locus_tag="PWN45_002525"
     CDS             complement(1794..1967)
                     /locus_tag="PWN45_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624262.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            2019..3398
                     /locus_tag="PWN45_002526"
     CDS             2019..3398
                     /locus_tag="PWN45_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL0624263.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(3518..4546)
                     /locus_tag="PWN45_002527"
     CDS             complement(3518..4546)
                     /locus_tag="PWN45_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL0624264.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            4816..5217
                     /locus_tag="PWN45_002528"
     CDS             4816..5217
                     /locus_tag="PWN45_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDL0624265.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
BASE COUNT         1791 a          877 c          813 g         1753 t
ORIGIN      
        1 ctagcattta tgaatagatg ggagtttatt ttattattat ataggagaga tgttgaatga
       61 cacatcgcgc actattagtt gttgactatt catatgactt tatcgcagac gacggcttac
      121 taacatgcgg taaacctgga caaaatattg aagattttat tgtttctcgt atcaatgact
      181 ttaattatta tcaagaccat atattctttt taatggattt acattattta catgacattc
      241 atcatcctga aagtaaatta ttcccaccac acaatatcgt agatacaagt ggtagagaat
      301 tatacggtaa agtaggtaaa ttatacgaaa caattaaagc gcaacctaat gtacatttca
      361 ttgataaaac gcgctatgat tcgttctttg gtaccccgct tgatagttta ttgagagaaa
      421 gaagtattaa tcaagtcgaa atcgttggtg tatgtaccga tatttgcgtg ttacatacag
      481 caatttctgc atacaactta ggttataaaa tttcagtacc tgctgaggga gtggcttcat
      541 ttaatcaaaa agggcatgaa tgggcacttg cacatttcaa aaactcatta ggtgcagagg
      601 tagaacaaca cgtttaaatc gtgctaaaat aattataaag aatacaattt acaagggaga
      661 tatttgacaa tggctaaaac atatattttc ggacataaga atccagacac tgatgcaatt
      721 tcatctgcga ttattatggc agaatttgaa caacttcgag gtaattcagg agccaaagca
      781 taccgtttag gtgatgtgag tgcagaaact caattcgcgt tagatacatt taatgtacct
      841 gctccggaat tattaacaga tgatttagat ggtcaagatg ttatcttagt tgatcataac
      901 gaattccaac aaagttctga tacgattgcc tctgctacaa ttaagcatgt aattgatcat
      961 cacagaattg caaatttcga aactgctggt cctttatgtt atcgtgctga accagttggt
     1021 tgtacagcta caattttata caaaatgttt agagaacgtg gctttgaaat taaacctgaa
     1081 attgccggtt taatgttatc agcaattatc tcagatagct tacttttcaa atcaccaaca
     1141 tgtacacaac aagatgttaa agcagctgaa gaattaaaag atattgctaa agttgatatt
     1201 caaaagtacg gcttagatat gttaaaagca ggtgcttcaa caactgataa atcagttgaa
     1261 ttcttattaa acatggatgc taaatcattt actatgggtg actatgtgac tcgtattgca
     1321 caagttaatg ctgttgacct tgacgaagtg ttaaatcgta aagaagattt agaaaaagaa
     1381 atgttagctg taagtgcaca agaaaaatat gacttatttg tacttgttgt tactgacatc
     1441 attaatagtg attctaaaat tttagttgta ggtgctgaaa aagataaagt tggcgaagca
     1501 ttcaatgttc aattagaaga tgacatggcc ttcttatctg gtgtcgtttc tcgaaaaaaa
     1561 caaatcgtac ctcaaatcac tgaagcatta acaaaataat actatattac tgtctaatta
     1621 tagacatgtt gtatttaact aacagttcat taaagtagaa tttatttcac tttcaatgaa
     1681 ctgtttttta tttacgtttg actaatttac aacccttttt caatagtagt ttttattcct
     1741 ttagctaccc taacccacag attagtgatt tctatacaat tccccttttg tctttacatt
     1801 ttcttaaaat atttgcgatg ttgagtataa atttttgttt tcttcctacc tttttcgtta
     1861 tgattaaagt tataaatatt attatgtaca cgattcatcg ctctattttc aactttcaac
     1921 atatataatt cgaaagacca tttaaaatta acggccacaa cattcaaatc aattaatcgc
     1981 tttttccaaa ataatcatat aaggaggttc ttttcattat gaatatcatt gagcaaaaat
     2041 tttatgacag taaagctttt ttcaatacac aacaaactaa agatattagt tttagaaaag
     2101 agcaattaaa gaagttaagc aaagctatta aatcatacga gagcgatatt ttagaagcac
     2161 tatatacaga tttaggaaaa aataaagtcg aagcttatgc tactgaaatt ggcataactt
     2221 tgaaaagtat caaaattgcc cgtaaggaac ttaaaaactg gactaaaaca aaaaatgtag
     2281 acacaccttt atatttattt ccaacaaaaa gctatatcaa aaaagaacct tatggaacag
     2341 ttttgatcat tgcaccattt aactatcctt ttcaactagt gttcgaacct ttaatcggtg
     2401 ctattgcagc aggtaatacg gcaattatta aaccatctga gttgacgcct aatgttgcac
     2461 gagtgattaa acggttaatc aatgaaacat ttgatgcaaa ttacattgaa gttattgagg
     2521 gaggaattga agaaacgcaa acgttaattc acttaccttt tgactatgtc ttctttacag
     2581 gaagtgaaaa tgtaggcaaa atcgtttatc aagctgccag cgaaaattta gttcctgtga
     2641 cattagaaat gggcggtaaa tctccagtca tcgttgatga aacagcgaat attaaagttg
     2701 ctagtgagcg catttgtttt gggaaattca ctaatgccgg ccaaacatgt gttgcaccag
     2761 attacatttt agtacacgaa tctgtaaaag atgatttaat cacagcccta tcaaaaacgt
     2821 tgcgtgaatt ttatggtcaa aatatacaac aaagtccaga ttatggccgc attgtaaacc
     2881 ttaaacatta tcatcgtctg acttcattac ttaacagtgc acaaatgaat attgtatttg
     2941 gtggtcatag tgatgaggat gaacgttata tagaaccaac attgttagat cacgttacaa
     3001 gtgattcagc aattatgcaa gaagaaattt ttggtcctat cttaccgatt ttaacgtatc
     3061 agtcattgga tgaagcaata gcctttattc accaaagacc aaaacctttg agtttatatt
     3121 tatttagcga agatgaaaat gctacacaac gtgtaataaa cgagctatca tttggcggcg
     3181 gcgctattaa tgatacattg atgcacctag cgaatcctaa attaccattt ggtggtgttg
     3241 gcgcctcagg tatgggacgc tatcatggta aatattcatt cgacactttt acacatgaaa
     3301 aaagctacat tttcaaatct acacgattag aatcaggtgt ccatttacca ccatataaag
     3361 gtaaatttaa atacatcaaa gctttcttta aaaattaatt tttgagacaa aaataaacca
     3421 tgcttccata atttgatgtt cttgaaaaca ataaatttca aggtatacac aaattttttg
     3481 gaaacatggt tttcttttta tcatgattaa ttaaagatta aaatatgaca catacacctt
     3541 cagatgctac acgagtgtta tcacatagcg taattttacc tgtttcttta tttctttcga
     3601 aaacagttac aactgaatct ccttgctcat gagcacaaac taaaaggtca tctgatgagg
     3661 caatattaaa atctcttggg aattgaccac cactttcagt aattgttact agttctaagt
     3721 gttgaccatt atcaagaact ttaaaaattg caatgctatc atgccctcta ttagatacat
     3781 ataagaattg ttgatcatga gataaacgca ctgctgcaag tttagtatct ccatcaaagt
     3841 tttcaggaat tgttaaatga cgctcgagct cttcaaattt accgtcatta tattctgcaa
     3901 cactcacagt atttgataat tcgtgtacga cataagcaaa ttttccatta tcatgaaatt
     3961 caatatgtct tgtcccatca ctatctttaa ataaagattc tttataaaat tcaaacccgt
     4021 tgtcatcaaa tttataagta acgatacgat cagcacctaa atctgttact gcaacgtact
     4081 taccatctgg agtttgatta atataatgtg cgtgtggatg atcttgtctt tcatgtgtac
     4141 ctgttggaaa atcatgtgca agttcttgaa ttagacgtat aatttcacct gtgtgcgtat
     4201 ttaattcata catacgtatg atgccagcac catataccgc ttcaaataaa tatcgtttat
     4261 cttctgaaat cgatacataa caacctgtac cagcttttga agacaaacat ttatttaata
     4321 aatgcaattc accattgtca tcaattttca aacttgcaac accgcattgt tctccttctt
     4381 tgttgattcc atataaaact tcattattac gcaccaaata tgtagacgct tctaattcaa
     4441 atcctgtttc taataaatca atacgtgact gattttcgtt taattcaaaa cgataaatcc
     4501 ctttaccatt ctttttagtg taagaaccaa tatatccatt tgtcattact tttacccctc
     4561 catgttagtc ttattttcat tttattaaat tttagctaga attaacaatc atttatcatt
     4621 tccttaaagc taaaactatt taactacttt aaaagaacta tttgctgtga tatgtcatta
     4681 ttcgatttta attaatccct cttttttact tacacgagca ttaattatta acgcgtttat
     4741 gcttccacaa aatttttgtt gcaaaataga acacatgttc gtataatgta attatcagat
     4801 tggggtgaat taaggatgac aagttatgaa gaagaaactg attatcgtaa cgttccaaga
     4861 gaattactag ataacaatat acctatggga agaggcatga ttaaatgggc tccttttgca
     4921 acattacccg aacaattcga aacgattcaa caatacatta tcgaccaaaa caaaattaca
     4981 cgtcctgtat tatcagatga tcaattggct gagctaaaca ttcggctaca cgaggcgcta
     5041 caatatgccc aacctgtaga agttaaattt tataataatg gctttgtaga ttcagtacgc
     5101 ttaaccattt atcgtattga tgctattaat tatgaaattg atggttatgt ttacaatcag
     5161 caacaacgtc aaaaaatttc tatctttgat atcttagata ttgtcttgtt accttaatgc
     5221 tttgaacata atta
//
LOCUS       DANJFZ010000151         5154 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.152, whole genome shotgun sequence.
ACCESSION   DANJFZ010000151 DANJFZ010000000
VERSION     DANJFZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.152"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            187..693
                     /locus_tag="PWN45_002529"
     CDS             187..693
                     /locus_tag="PWN45_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624266.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            952..1449
                     /gene="efb"
                     /locus_tag="PWN45_002530"
     CDS             952..1449
                     /gene="efb"
                     /locus_tag="PWN45_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDL0624267.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1603..1953
                     /gene="scb"
                     /locus_tag="PWN45_002531"
     CDS             1603..1953
                     /gene="scb"
                     /locus_tag="PWN45_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDL0624268.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(2606..2791)
                     /locus_tag="PWN45_002532"
     CDS             complement(2606..2791)
                     /locus_tag="PWN45_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624269.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(3413..3646)
                     /locus_tag="PWN45_002533"
     CDS             complement(3413..3646)
                     /locus_tag="PWN45_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624270.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
     gene            complement(4123..5082)
                     /gene="hyl"
                     /locus_tag="PWN45_002534"
     CDS             complement(4123..5082)
                     /gene="hyl"
                     /locus_tag="PWN45_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDL0624271.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT         1852 a          716 c          746 g         1840 t
ORIGIN      
        1 attgaattat gcattttcga agtaaacata attcaacact tacattttta aatagataaa
       61 atagtgtaaa atcacgtgcc aagattacta tagagtaggc attgagtatt attggcaatc
      121 acttaagtga ttgttatttt tcgtggtcaa taataaagat ataaataaat atattgaggt
      181 caaacgatga taattaaaat tttaacaatt ctattactac tttgtatatt gagctatttg
      241 gttacaaata gaaagaagcc ttttctgttc ttaaagacac tctttatggg tgtggtattt
      301 atctttatag gatatatttc actggcaata tctgccgtaa ttatttatgg tattattcaa
      361 tttatcacaa ttgattttgg tagttttttc ttaatgggta ttatattgat cttgatttca
      421 agtatattcc aattatttat agttagatta ctttttagaa aaaagaatgt cgatttgaca
      481 gaggttgtcg ttttagagca tttaattcaa tggttcttag tttactttgc gatctatcaa
      541 gcagtaaatg aaaaaatgga cattaatgat attaatatcg acaatttcca atctgtcttt
      601 tttgacgtgt ctaatttgaa tttagtaatt ctaccaacgt taatcattag ctgggtcaca
      661 atatttaact atagaatgag aagttacaaa taaaatctat gagattatac cttcagacac
      721 caacattcaa atggtgtctt ttttgttgtg tggttttatt tttgaaattc gaaaaagtag
      781 aggcatgaat tttttgacta gtgtataagt gctgatgagt cacaagatag atagctatat
      841 tttgtctata ttataaagtg tttatagtta attaataatt agttaatttc aaaagttgta
      901 taaataggat aacttaataa atgtaagata ataatttgga ggataattaa catgaaaaat
      961 aaattgatag caaaatcttt attaacatta gcggcaatag gtattactac aactacaatt
     1021 gcgtcaacag cagatgcgag cgaaggatac ggtccaagag aaaagaaacc agtgagtatt
     1081 aatcacaata tcgtagagta caatgatggt acttttaaat atcaatctag accaaaattt
     1141 aactcaacac ctaaatatat taaattcaaa catgactata atattttaga atttaacgat
     1201 ggtacattcg aatatggtgc acgtccacaa tttaataaac cagcagcgaa aactgatgca
     1261 actattaaaa aagaacaaaa attgattcaa gctcaaaatc ttgtgagaga atttgaaaaa
     1321 acacatactg tcagtgcaca cagaaaagca caaaaggcag tcaacttagt ttcgtttgaa
     1381 tacaaagtga agaaaatggt cttacaagag cgaattgata atgtattaaa acaaggatta
     1441 gttaaataaa acttcaatcg ttgctgttat ctggaaataa ttaattaaat gttatgttaa
     1501 tttttgttaa tgaaaaaagt aatctattta atgacaggtt aatgtaattg tcctgaaatt
     1561 gactatatac tcagtaagta tcaattttaa ggagagctta taatgaaatt taaaaaatat
     1621 atattaacag gaacattagc attactttta tcatcaactg ggatagcaac tatagaaggg
     1681 aataaagcag atgcaagtag tctggacaaa tatttaactg aaagtcagtt tcatgataaa
     1741 cgcatagcag aagaattaag aactttactt aacaaatcga atgtatatgc attagctgca
     1801 ggaagcttaa atccatatta taaacgtacg attatgatga atgaatatag agctaaagcg
     1861 gcacttaaga aaaatgattt cgtatcaatg gctgatgcta aagttgcatt agaaaaaata
     1921 tacaaagaaa ttgatgaaat tataaataga taataaataa aacaggttga gacaaaaaat
     1981 ggtcttaacc tgttttcaat ttgcatatgt gataaattct atatcaaaat gcttatgtat
     2041 aatgaatgac atttaaaagt aggggagaca aatataaata caatagttcc taggattact
     2101 ctcaaaataa ctatatcaat tatttacttt gctctcctat tttttaaaat atctacatgt
     2161 ttaaacaatc aaaagtgtac aatattaaat tatcatttcc agttctagtg ctatattggt
     2221 agtagttgac taaatgaaaa taagcttata acaagttttt tcaatactcg tggggccaca
     2281 acagagagaa ataggatcac caattccaac aaacaatgca agttggcggg ggccccaata
     2341 tagaagctgg cgaaaagtca gcttacaaaa atgtgcaagt tggcggggcc ccaacataga
     2401 gaaattggaa ccccaatttc tacagacaat gcaagttggg gtaggacatc gataaagaaa
     2461 tactttttct ttagcaatta gtatttctta tgcatgagct ttactcatgt attcattttt
     2521 taagtacaca ttagctacag ctaatgataa agaaccacta cataataaat cattagtggt
     2581 tctttatcat ttctatctca ctcttttact ggaagaaaaa gtttacgttt gtagaacatg
     2641 ccacaatacc aaaaataatt aagaaaaata agacgataag catgatgaca cttttcaaac
     2701 aacctctatc agtttctttc gattttcttt gttgaacctt tttataatct tcaagtagtt
     2761 ttgcggcttt tttatttata tgtttattca tgatgttgac tccttataat atatgtttaa
     2821 ttcattaaaa tagttgaaaa catgacttga aataaagata taaagctaaa gtaaggatgt
     2881 tcaattgttt caaattttat gaaagtataa cgttaaatat gttaatttga tgatgatatt
     2941 tgctttttat tttccaaatg gaatttactt aaactgatgc attaaaatat taatgaagca
     3001 ctagaataca taaatgaata gtaatggtgc acagtataga ataattaagg ctatattaag
     3061 tataaatatc gttaactgta agctatcttt agttttaata taaactatta ggataatcga
     3121 cgtaagaaga atcatatata ttaatgatga agaagtccat acaaaatccg catcatttgt
     3181 tgttaataat gggactataa ttaatccgaa attaatcatg catgctatat atactataat
     3241 gttatacaca atgttaattt ttgttcacca ccttatactt ctattttaaa aacttcttta
     3301 taatgatata ttgtttaatg ttgaaataat tagattatct aattttcatt tgctttacat
     3361 gtaaaaggct atatatagta tgctctttat gattctaaat gctttttaat atttaatgct
     3421 catcaacatt tggattttga atattcaatt caaaaacttt attagctacg tcaattgtaa
     3481 aatcagaacc atagttgaca tgagctactt ttaattttcc atctaaataa tagattgcga
     3541 ttgcaacatc gtaaaattcg tcaatgacaa ataaactctt ttcgtttgtt acaacctcat
     3601 gctctcctga gtatacaacg ttaatttccc aatcattaaa aaccatttgt taacctcctt
     3661 gaacatttaa attgattcaa cttaagttta acttattcat acaacttcgt acaatatcta
     3721 gatgaacatt aattgtattt ctagaaatct ttttcaatta tatgtactaa ttatactttt
     3781 aaatttctta tttcagtata gttttaaaac gattttaaaa taattctgca aatatattaa
     3841 cacataatgt gttcaaaaag ttttgaacaa tttcaaaact tttatataaa gggtttgaca
     3901 acatggaatt caaatttctt attttaaaaa ttacctcata tagtgtcatg tttagtctaa
     3961 tttttaagtc aggaaagcaa atcaagtaga tagtttaaat taaaaatatc ataagttaat
     4021 taaactaaat tcagtataaa aattagccga aaaacatcat ttctgaagtt atcggctaaa
     4081 gttataaatt atatttattt gtacatgaac aaataattta cattaatttg tcatttcttc
     4141 tttttcccaa tcgattttat atctttctga agaacgatct atccatttat ctttagtatt
     4201 ggtacctttc caatttgttg aagtccagtg caattggtag tcatcacgaa ctcgttcgta
     4261 tattacatct atatttgttt gttgtttgga tgcttttcta tccatagtaa taactgtagc
     4321 gaagtctggt gaaaaccctg aagataatag agaacttgct ttgttaggat caaggaagtt
     4381 atctgctgct ttcatagagc catttctagt tttcatgaaa agttgattgc catataccgg
     4441 gttccaagaa tctctatcat atggtcccca attttgattc accatattgt taaatatcac
     4501 tttccagcct acttttttat cagttgggct ctctaaaatt gttttgaaat caggttgaac
     4561 atatttcagt gtatgaccaa tcgaaacatt tgcaccaata aggccgccaa tttttcctgt
     4621 atcatcacca gtaacattac cgttgaatcc ataagttaaa gtactcatat actcttttgt
     4681 atcaatcgaa tttcttggat agtaatcaga tatttgagct acttcattat caggtagttg
     4741 caactgtacc ttaaaggctg aaggccaggc taaaccactt ttgttagcac cttcttcgct
     4801 ataaactcta tattgaccag caatggtacc tttcgttcta ataactagca gttttttatt
     4861 atgattttta tcatcgataa aactataaaa tacttttttg tgcatgccat tttctttatc
     4921 ataagtgact aaatcacctg tttttactgt agtattgctt ccaatatctg tagtaccggt
     4981 tttaatatta atatcagaat ctgcggcatt agcgacagga ttcattaata tggaacctag
     5041 caatagtgtt gttgttactg agctgactat acgtgttttc attttcatca tccttctatt
     5101 ttttaaaacg atttgaggaa acaataatca atatgtcaat ttagaatatt gcag
//
LOCUS       DANJFZ010000152         4795 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.153, whole genome shotgun sequence.
ACCESSION   DANJFZ010000152 DANJFZ010000000
VERSION     DANJFZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.153"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            10..780
                     /gene="fabI"
                     /locus_tag="PWN45_002535"
     CDS             10..780
                     /gene="fabI"
                     /locus_tag="PWN45_002535"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL0624272.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(975..2060)
                     /locus_tag="PWN45_002536"
     CDS             complement(975..2060)
                     /locus_tag="PWN45_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0624273.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            2402..3970
                     /locus_tag="PWN45_002537"
     CDS             2402..3970
                     /locus_tag="PWN45_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL0624274.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            4112..>4795
                     /locus_tag="PWN45_002538"
     CDS             4112..>4795
                     /locus_tag="PWN45_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL0624275.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWK"
BASE COUNT         1596 a          675 c          892 g         1632 t
ORIGIN      
        1 ttatcttaca tgttaaatct tgaaaacaaa acatatgtca tcatgggaat cgctaataag
       61 cgtagtattg cttttggtgt cgctaaagtt ttagatcaat taggtgctaa attagtattt
      121 acttaccgta aagaacgtag ccgtaaagag cttgaaaaat tattagaaca attaaatcaa
      181 ccagaagcgc acttatatca aattgatgtt caaagcgatg aagaggttat taatggtttt
      241 gagcaaattg gtaaagatgt tggcaatatt gatggtgtat atcattcaat cgcatttgct
      301 aatatggaag acttacgcgg acgcttttct gaaacttcac gtgaaggctt cttgttagct
      361 caagacatta gttcttactc attaacaatt gtggctcatg aagctaaaaa attaatgcca
      421 gaaggtggta gcattgttgc aacaacatat ttaggtggcg aattcgcagt tcaaaactat
      481 aatgtgatgg gtgttgctaa agcgagctta gaagcaaatg ttaaatattt agcattagac
      541 ttaggtccag ataatattcg cgttaatgca atttcagctg gtccaatccg tacattaagt
      601 gcaaaaggtg tgggtggttt caatacaatt cttaaagaaa tcgaagagcg tgcaccttta
      661 aaacgtaatg ttgatcaagt agaagtaggt aaaactgcgg cttacttatt aagtgattta
      721 tcaagtggcg ttacaggtga aaatattcat gtagatagcg gattccacgc aattaaataa
      781 tatcattcaa cagctttgtt cacgttatta tatatgtgag caaagctttt ttgcttttat
      841 aataatcgga ctgatggaaa attatttgat atttcatttg actgattttt tatgcacaaa
      901 aaaatcgcct caaaagctgt agtttcaact tttgatgcga tagtatctta tattcttaaa
      961 agacatccac agaattagtc cttaacatta ctgtttgctt tatcaataat gcgttggcgg
     1021 tatttgaaaa tattactaac aaccgtttta agtacagcat ataatggcac tgcaatcaga
     1081 actaatgtaa agccacctaa atctcctgct gctaaaataa caacgataat tgttaaagga
     1141 tggatactta aagatttacc cattacattt ggtgtaatga tattaccttc aagttgatgt
     1201 gcaattaatg taatgataca aacccatata aatgtagtag gactatctat aataccgagt
     1261 attgctgcag gtgcaaatga taaccatgaa cctaagaaag gaattaagtt tgcgacacca
     1321 gcaaatagta ctaataaagg aatatatggt aagtcaataa ttgaataacc gatatataag
     1381 aatataccta aaataacact gacagttact tgaccttgaa tgtaagattt taatgtaaag
     1441 tttaaatcag ttaataaatc tacgaaaaat actttacgtt cacctttgaa aaatttagca
     1501 acagctggga taaatttttc atggtctttt aacatataaa ttaagaagaa tggaaccata
     1561 atcaataaga agatggttga aattaatgat gtaatgtact gtaatgaatt agataaaata
     1621 ttagtaacgc catcacccat tgatttaacc atatttgtaa ttctacttgt tacatcttca
     1681 ggtaatttat ccatttgagc tagtgcgaat ttaataattt gctctgcttc tttttgtagg
     1741 gcaggtgtct gactaattaa attgttgata tttgaaatga tgattggtgc aacaaacgca
     1801 acaattaagc cgataatagc aaacaaagct aacatgattg ttgttatact agcccatctt
     1861 ggaaaaccga ctttttcaag taagttttga aaaggtagac agatataaaa taaaaaacca
     1921 ctaattaaaa atggaagaaa tacagaaccg atgattgtag ctattggagt aaatacttcg
     1981 tgcacttcca taaatagttt gatgagaatg aacagcataa tcagagcgat gccagttcgg
     2041 aaccaaacct tgtttaacat aaacgatgtt cctcctattt ttctaaactt agttattatt
     2101 tagtatatcc taaatagcag tgcttaaaaa ggttaatatt ttttaaatat acttaaatta
     2161 accattcact ttctatttta agatatttac tttcaatagt ttgtgagact tttcaatgac
     2221 tcgtaagtct gcaattggat caaacagcac cagtttatat gaataactta ttttaatatt
     2281 aaacgtgttt aaatagtgaa atgatcatat taatatttat aagaatattc tgaaaaatat
     2341 attgaagtgt ctcgtgctta atcgtataat atttttatgt ttaaacaata gggggaatct
     2401 tatgattgaa aaattagtaa cctttttaaa tgaggttgtt tggagtaagc cattagttta
     2461 tggtttgcta attactggtg tgctatttac attgcgtatg cgattttttc aagttagaca
     2521 ttttaaagaa atgattcgat taatgtttca aggagagaag tctcctaatg gtatttcaag
     2581 ttttcaagcg atagccatgt ctttagcagg cagggttggt acaggtaata ttgtcggtgt
     2641 atctactgca atatttatag gaggacctgg tgcagtattt tggatgtgga ttactgcgtt
     2701 tttaggtgca agtagtgctt ttattgaatc tacacttggt caaatattca agagagttga
     2761 aaataatgaa taccgtggtg gaccagcgta ttatattgaa tatggtattg gtggtaaatt
     2821 tggtaaaatt tacggaatta tctttgctat tgttacgatt atctcagtag gtctattgct
     2881 tcctggtgtg caatctaacg ctatagcaag ttctatgcat aatgcgattc atgttccaca
     2941 atggttaatg ggtggtattg ttgtagttat tttgggatta attatttttg gtggtgtacg
     3001 tagtattgcc aatgttgcaa cagccgttgt accatttatg gcaattattt acatactgat
     3061 ggctgtcatt atcatttgta tcaatataca agaagtgcca gcgttatttg cattaatttt
     3121 caaatcagca tttggattac aatctgcttt tggtggtatc gttggcgcaa tgatagagat
     3181 tggtgttaaa cgtggattat attcaaatga ggctggtcaa ggtacaggtc cacacgcagc
     3241 agcggcagca gaagtatcac atccaagtaa acaaggtcta gtacaagcat tttcagttta
     3301 tattgataca ttatttgtat gtactgcaac tgctctgatt atacttattt ctggtacata
     3361 taatgtgact gatggtacgg ttaatgcgaa tggcacaccg catttaatta aagatggcgg
     3421 tatttatgtt gaaaatgcaa caggtaaaga ttattcaggt actgcgatgt atgcacaagc
     3481 cggcattgat aaagcgttcc atggcagtgg ttatcaattt gatcctactt tctctggcgt
     3541 aggttcgtac tttattgcat ttgctttatt cttctttgca tttactacaa ttttgtcgta
     3601 ctactacatt acagaaacaa atgttgctta tttaacgcgt aatcaaaata atcaagtttc
     3661 atcgatattt attaatattg ctcgtgtgat tattttgttc gctacatttt acggtgcagt
     3721 taaaacagct gatgtagcat gggcattcgg tgatttaggt gtaggtctaa tggcttggtt
     3781 aaatatcatt gcgatttgga ttttacataa gcctgccgta aatgctttaa aagattatga
     3841 aattcaaaag aaacgtttag gcaacggtta taatgcagtt tatcaacctg atccgaataa
     3901 attacctaat gctgtctttt ggttgaagac gtatccagaa cgtttaaaac aagcacgtgc
     3961 caaaaagtaa tctacttttg tttatagtat atgtagtgat catttgataa aaaagaaaag
     4021 tattgagaat tttaggtgct cagaaatttg aattttaaaa atatagtgtc tcttggtaca
     4081 ataacaatac aactactagg ggcacttttt tatgtcagaa tttaaaactg gtaagattaa
     4141 taaacatgtt ttatatagta atattttaaa tagagatgtc acgttaagta tttatttacc
     4201 agaatcttat aatcaacttg ttaaatataa tgtcattctt tgctttgacg gattagattt
     4261 tttacgtttc gggagaatac aacgtacata tgaatcgtta atcaaagaag cgcgtattga
     4321 tgatgcgatc attgttggat tccattatga agacgttgat aagcgtagag aggaatttca
     4381 tccacaagga agtcgttctc atttaactat tcaatcagtc ggtaaagaaa tattgccatt
     4441 tattgactcg acgttttcta cactgaaagt aggtaatgca aggttattag taggggatag
     4501 tttagcgggt agtattgcct tattaacggc gttgacctat ccaacgattt ttagtcgtgt
     4561 agcaatgtta agtccacatt cagatgaaaa agtattagat aagctaaatc aatgtgcaaa
     4621 taaagaacaa ttgacaattt ggcatgtcat tggtctagat gaaaaagatt ttactttacc
     4681 aacaaatggt aagcgtgccg atttcttaac accgaataga gaattagctg aacaaattaa
     4741 gaaatataat ataacttatt attacgatga atttgatggt ggtcaccaat ggaaa
//
LOCUS       DANJFZ010000153         4735 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.154, whole genome shotgun sequence.
ACCESSION   DANJFZ010000153 DANJFZ010000000
VERSION     DANJFZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.154"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            298..1101
                     /locus_tag="PWN45_002539"
     CDS             298..1101
                     /locus_tag="PWN45_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0624276.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(1118..1360)
                     /locus_tag="PWN45_002540"
     CDS             complement(1118..1360)
                     /locus_tag="PWN45_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624277.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(1534..2934)
                     /gene="nhaC"
                     /locus_tag="PWN45_002541"
     CDS             complement(1534..2934)
                     /gene="nhaC"
                     /locus_tag="PWN45_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0624278.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(3029..4111)
                     /locus_tag="PWN45_002542"
     CDS             complement(3029..4111)
                     /locus_tag="PWN45_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDL0624279.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            4359..>4735
                     /locus_tag="PWN45_002543"
     CDS             4359..>4735
                     /locus_tag="PWN45_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDL0624280.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKY"
BASE COUNT         1628 a          897 c          665 g         1545 t
ORIGIN      
        1 agaattttca gtaatattac aaatttgtaa cagacttatt ttcgaaacat tcagttttca
       61 caaagaatgc cgtagttata agttttgcaa aatgcaaatt tctatacttt taaaattcta
      121 ttgtgtcaag atattagcaa aaaattacaa aacgttaaca aagcacacaa ggacgctaat
      181 ttctgttata gtctttcttg tcgtttaatt acgagagata acttataaca ttaattttgt
      241 cacacggaca ttacgaaaat attttagatt taaaattttt aggaggatat ttttaaaatg
      301 aagaaaatcg ctacagctac tatcgcaact gcaggattcg ctacaatcgc aattgcatca
      361 ggaaatcaag ctcatgcttc tgagcaagat aactacggtt ataatccaaa cgacccaaca
      421 tcatatagct atacttacac tattgatgca caaggtaact accattacac atggaaaggt
      481 aactggcatc caagtcaatt aaaccaagat aatggctact acagctatta ctactacaat
      541 ggttacaata actataacaa ttacaacaac ggttatagct acaataacta cagccgttac
      601 aacaactact caaataataa tcaatcatat aactacaata actataatag ttacaacaca
      661 aacagctacc gtactggtgg tttaggtgca agctacagca cttcaagcaa caatgttcaa
      721 gtaactacaa ctatggctcc atcatcaaat ggccgttcaa tctcaagtgg ttatacttca
      781 ggacgtaact tatacacttc tggtcaatgt acatactacg tatttgatcg tgtaggtggt
      841 aaaatcggtt caacttgggg caatgcaagt aactgggcta acgcagctgc aagagctggt
      901 tacacagtga acaatacacc aaaagctggt gcaattatgc aaacaactca aggtgcatac
      961 ggtcacgttg catacgttga aagtgttaac agcaatggtt cagtaagagt ttcagaaatg
     1021 aactatggtt atggcccagg tgttgtaact tcacgtacaa tctcagctag ccaagctgct
     1081 ggttataact tcattcacta attagtgatg gctatgttta cgccaacaag gtttcaagag
     1141 ccttgttggc ttttttaatt tctatgaaaa aaatcgaatt ggtaaaaatc tcaaaatttc
     1201 gattttcacc aatccgattc actctgtaat aaagaataaa caacatacag ccattgacac
     1261 acccaaattt aggcatgtca aatcggctat acattgataa atttttagtt aaagtactta
     1321 caaatatact tgccttcaaa ttctcaatta atttagtcat tacaaatcat gacataaaac
     1381 ttacattaca tatatcaaca tgttccgcta caaaacatat tcagtatatg tatagaggat
     1441 atttattcta agttaaaacc ttttcaaatt ataaccgcta caattataaa tgattaaagt
     1501 gtttcaacat attatatata ttccacgctc acattaagtt aaacgtgaat tcgatgactt
     1561 tttaatacct atccctgtaa aaccatagat aattgctata attgcgcata aataacatgg
     1621 tactgtccat atgaaaaatt cttcaacaga gacatgaagt tgattcgtat agtaaatacc
     1681 tgatgttccc catggtatta aaggtaagac catcgtactt gagtcttcaa gtgttcttga
     1741 taaaacggaa cgagagactt ggtatttttc gaacaaatct ttcattaaca caccgaccat
     1801 aataattaca atcgaagcaa cacctgcagc gaatacaagc gcaatacaac aaataacagt
     1861 aatacatatt aaacttccta cagaatggat gcctttagaa atagtagtta ataagacttc
     1921 taaacatcct gctttttcta caatacctgc aaatgcatat ccgcaaaata tcgttactaa
     1981 tatttgggtc atactcatca taccaccctg ttctaacaag cttttcacac tggatgaaat
     2041 atgagactga tgtatcattg attcgttaaa accactaaat gttgctttga aaccatctgt
     2101 cattttgaaa tgatgattaa aagtccccac tataatggca gaaaagcttg atattagcat
     2161 agctggcact gtagccattt taaatagcaa acaaacaatg atcacaatta agggaaccca
     2221 gacccatatg ttaatttgat aaatctgtgc aagctctgat aacaaagttt gaatctgttt
     2281 atcatttgaa tgccctttaa attgaaatcc agcaataaac catactaata aacctatgat
     2341 tgatgcaggt atcgtcgtcc acatcatcga atgtatatgt ttaaatatat taactttagt
     2401 aacaagcgcc gctaaattag ttgtatctga taatggtgac attttatcgc caaacacagc
     2461 ccctgctata atagcacccg ctgccatccc tggaggaatc cccaattgat taccaataga
     2521 aataagtgca atccctgcag ttgatgcaga gccccatgct gtaccagttg ctacagatgt
     2581 aacagcactt ataaaaaagg ctgatattaa aaaatagctt ggattcaata aatctaagcc
     2641 ataataaatc aaggctggca ctgtgccaga aaacatccaa ctgcctacta taattcctac
     2701 cgctagtata attaaaattg caggcattgc agtatttaaa cgttctgcaa tgcctttttc
     2761 taaatcttgc catgttaagc ctacacgttt tgcaatccat gtggcatatg ctgaggcaat
     2821 aattaataat acttgaattg gaatatcaaa aaacacaaag cctgttacaa caacaataac
     2881 cattacaatc attgtcgaaa ttgattctaa aaaggtgggt tttcttttca tcatctatcg
     2941 tccccttcgc ttattgatga gaaacctctt ttcttcaatt tattgctttt gcataatgct
     3001 atatcctttt ctatcttcaa gccatcattt attttaaata tttttctaaa tcaagcttat
     3061 ctaaaccaat ttcttcaact gttaagcctt cctcaaagaa gtctctctct aaaatggttg
     3121 atgcaattac aattactgca tctatatttg gtgtcggtac atcaataaca cgacctaagc
     3181 ttgaccatag tactaaacca tatgcaatat cttcagttaa atatctgctt tctacatgat
     3241 ttggtcctgg aatttgtgaa aatactgggc ttgtattaaa caaacgattt aatggttcat
     3301 cttctttatc acgttctaaa taaccacgtt caatacgtga ttctttagct gttgataatt
     3361 caaaacctaa tctacggcct aaattcaaac gttctaattc gattgcatga agtaatctaa
     3421 ctgtatgttt agtaattcct tctttatata aagcgaactc gccagcataa tcaatgcgac
     3481 cgacattcaa taatgttggt cctggatgca cttcaggatt accattttca agatttgttt
     3541 taattaagct ttcctctttt actaaatgat cataaatact tgaaactttg tcataacaat
     3601 catttagaca gcttctatca tatgttgaaa agaagatacg acgtacattt agagataaat
     3661 caactgctgc attttcaaaa tcgacacgcg taccatacgt caacgtatta gcttccgcta
     3721 gttgtggttt tgtttcaata tgtctatctt ctaaaacatt cataaaacga attgacccca
     3781 ttgctgcagc catgttgaag aatatcaact gattatcagt tacatgctct gccattacat
     3841 cagcataata ctctatgtat gaagatggaa taatcacttg aacaatttca gcatctttta
     3901 aaacatattc catatcatca ctaatatcag tgaattttac gaaacgttca tcaccttcat
     3961 tattaaaatc aaatccgccc ttttcgattg cgttttgaaa cttacttata gattgattac
     4021 gacaatataa tttaacatcg tggcctttgc tcaccatatc tactgctgcc gtaactgcgc
     4081 catttcctga tcctacaatt gctattttca taaacaaccc tctttctatc tttggttaaa
     4141 attaaaaaca aattatacgt aagacaatat aaaaatgatg cgaattcatt acgcatcatt
     4201 ataaatgtta agtatatata accttaaatt aacctattta aacgccgacg tccaccaatt
     4261 atttttattt ttaataattg tttaataaac atgtttattt ttgattatat tttgttatag
     4321 ttaaagataa tattacaatt ttcaaaggag tgtctaatat gacaactgaa atgaacgacg
     4381 taacaaatca tcaaccgaat cgcaaatcca atgatgagaa cattatggcg atgcttatct
     4441 atttattaag tttattcaca tctattattg gtccactgat aatatggtta ttgaaaaaag
     4501 atgaatcgaa actagttgat cgtgctggaa aaaattatct taactatacg atttcttata
     4561 ttatttggtc tatcgtttta gtcgttatca ctttaatagg cgttttccta attgctacag
     4621 atattgattt cattattatt ataggtttca tcattacgtt tatcggtatt ttatccatat
     4681 tcgctttctc aatattatct tttgtattca caattattgc gtgtgtcaaa tatta
//
LOCUS       DANJFZ010000154         4705 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.155, whole genome shotgun sequence.
ACCESSION   DANJFZ010000154 DANJFZ010000000
VERSION     DANJFZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.155"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(79..774)
                     /locus_tag="PWN45_002544"
     CDS             complement(79..774)
                     /locus_tag="PWN45_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDL0624281.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(1060..1740)
                     /locus_tag="PWN45_002545"
     CDS             complement(1060..1740)
                     /locus_tag="PWN45_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDL0624282.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            2212..3057
                     /locus_tag="PWN45_002546"
     CDS             2212..3057
                     /locus_tag="PWN45_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0624283.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            3076..3435
                     /locus_tag="PWN45_002547"
     CDS             3076..3435
                     /locus_tag="PWN45_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDL0624284.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            3959..4111
                     /locus_tag="PWN45_002548"
     CDS             3959..4111
                     /locus_tag="PWN45_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624285.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            4372..4644
                     /locus_tag="PWN45_002549"
     CDS             4372..4644
                     /locus_tag="PWN45_002549"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0624286.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
BASE COUNT         1597 a          762 c          677 g         1669 t
ORIGIN      
        1 aaagaagtta agcaacgttt gatcgtcact taacctctct atttcaattt caacttattt
       61 cgtcatcaag tatatgtgtt atgcttttat aactttgatt tcaattctat caatatctgt
      121 gacattgata acatcggaca tacggtcttc ttgtaacttt ttatccaatt caaatgtata
      181 ctttccatag tatttctttt tgactgtaat ttttcctgta ctcatttcac cgtaaagacc
      241 ataattatca ataaggtatt ttcttaattt aaaatcaatc tctttcaatg acatcgcttc
      301 tttatctatt ttaaatggga aaaagtcata atcatattca ccagtatgat cttctttaat
      361 aactcttgct tctgctatta ggtcgacagc tttatcgttt gcactcgtga tacccccaat
      421 agagtacttt gcaccttcaa atctcttatc ctcattaacg taaaatatat taagattacg
      481 atgtacaccc gtatgataat gttgcttatc tttgccaatt aaagcaatat tattaacaga
      541 attaccatct atgatattca taaatttaat acttggttga atgaaactga tataacctgt
      601 cacattttta tattcaatac taggttgatt ataataagct tttaattttt tgctattttc
      661 acttattaca ataggtttct tttcggcatg aactggtttt tccgttgtag tgtttacacc
      721 tgttgctaat attcctaata acaaacttat ttttgcaata tttttcattt tcatagttgt
      781 atgctccaat ctattataat tagattgttt tattacgtaa tttgaatcat acacccatat
      841 tataggagct gtattcggat attcacatta acctgttttt aactattcat aaaatatgat
      901 taagctattt aagcaaaaga taatgcgtgg tgcgactgtt tgtgtgagtt ttgtaacttt
      961 tcccctcaaa aaaagaagct aagcaacagt acgtcgctta acttccatat atttccataa
     1021 caagcactat tcaacatagc gctttgttgt tagaaagtat tatttcattt ctactagaat
     1081 tttattaatc ttagtgccgt cgattgactc acccatacgt tctttttcaa gtttttgact
     1141 taaatcgatt gtatgtgttg tgccatcatt cattgtaatt gtaatttgac cttgtttaag
     1201 accatttgaa tacaagccgt gttgtttaat cgcacgttct cttaatctat aatcaagttc
     1261 ttttaatgag atgtcttctt ttgaaatgta gtaaaaatct ttaagtacat cttcattatc
     1321 tttcttgatt tctaaaattg gcgtattgat ataatcaata tactgcacac tatttgattt
     1381 tgtaacgcca ccaattgagt aatttgttgc ttgtctactg tcaccttctc taaggataaa
     1441 cacatctata tttgagtttt ctccgtcttt aaatttatct ttatcagatc caagtaaagt
     1501 gattcgagaa tgctgttgcc ctactgtgac ttctaaatag tgtttaccct catcagtata
     1561 tttaaatcca gtcacatttt tacgctctaa aattggttta ttataatagt gttttaactc
     1621 tgattcactt tgttgtttaa cttctgcttt cgcttgtact gattgtacat tcgatgtaat
     1681 tacacctgtt gctaacattc ccaatgctaa acttgctttt gctatcgctt taaatttcat
     1741 aatttttagc cccaatctat ttaaatttga tttttcaatt acgtaatatg aacaatctac
     1801 ctacattata ttgaggcgct atcccctttc aaattaaacc tcatttaact aaacttgaat
     1861 cattgttaag gtaattaata aaatgctaac tgttattaat caattcttat ccgtcgagac
     1921 tcttttttat acaaatcaca ctttatacca tttttatttt aaaaactgta aaactttcag
     1981 acacaaaaca gacatcatat tgtcgtagta cctgattaaa attttcaatg atattagtga
     2041 tacacatcaa ctaaaacgcg tcattacggc attttcaaca ttaataatat ttacgtattt
     2101 actatacacg tcgacaataa atacagatta gaatttttta taaaatcaaa tgttttgaaa
     2161 accatcaaat aagtattatg ttatgtatat aaagattagg agatgaagtg aatgaatatt
     2221 atgttaacag gtgctacagg tcatttaggc acacatatta caaatcaagc cattgcaaat
     2281 cacatagatc attttcacat tggtgttaga aatgttgaga aagttccaga agattggcgc
     2341 ggaaaagttc ctgttcgaca attagattat tttaatcaag aaagcatggt agaagcattt
     2401 aaaggaatgg ataccgttgt gtttattcca agtattatcc atccatcatt caaacgtatt
     2461 cctgaagtgg aaaatttagt atatgcggca aagcagagcg gcgttgctca tatcattttc
     2521 ataggttatt acgcagatca gcataataat ccattccata tgagtcctta ttttggttat
     2581 gcagcacgtc tattggcaac aagtggcatt gactatacgt atgtaagaat ggcaatgtac
     2641 atggatccac ttaaaccata tttaccagaa ttgatgaata tgcataaact gatttatcca
     2701 gctggcgatg gtcgtattaa ttatattact agaaatgata ttgctagagg tgtcattgct
     2761 attattaaaa atccagatac ttggggcaaa cgctacttat tatcaggcta cagttatgat
     2821 atgaaagaac ttgctgcaat tttatctgag gcatcaggca cagaaattaa atatgagccc
     2881 gtttcattag agacatttgc agaaatgtat gatgaaccta aaggctttgg tgcattattg
     2941 gcatcaatgt acgacgcagg agcaagagga ctattagacc aagaatccaa tgatttccaa
     3001 caattagtca atgatcaacc acagacactg caatcatttt tacaagaaaa tatttaataa
     3061 taaaggagcg ttatagtgaa tatcatctca acaattttaa tcatatttgt ggcattagag
     3121 tttttctata ttatgtacct tgaaacgatt gctacaactt ccaaaaagac tagcgagaca
     3181 tttaatataa gcgtcgataa attgaaagac aaaaatatta acctactttt gaagaaccaa
     3241 ggcgtatata acggtttaat cggagttttg ctaatatacg gtttgtttat cagcagtaat
     3301 ccaaaagaaa tatgcgcagc tattttagtg tatatcattg gcgttgctat ttatggtggc
     3361 ctttcaagca atattagtat ctttttcaaa caaggcacat tgccagtatt ggcactcata
     3421 tcaatgcttt ggtaagtatt ggtgtttggg ggggtggaga tgtagtcgga ggtttggagg
     3481 atttgagcga attgtgtgtg gactttagac tcagagtatt tcatcctaat tatttcaagc
     3541 agaggtgaca gtagcgttgc ctctgtttcc ttatagaaaa attatttaat gaagaaaacc
     3601 catatctgat ttaattttca gctgataaat actccatata ttagaatggc tactttatct
     3661 attgcatcaa tcctttaaaa caaaaaaccc atgatttcga aattcccgta tgatggggtt
     3721 cctactctca tggatcagtt aaataaatat tatcactatc agtttattat ttcaatatta
     3781 ttaacaatat atgtagtcgt aaaaggaaag aggacatgag aacttcggtg ttgattggca
     3841 ttacataacg cttccaaaca tattatttgg taacaataag aaactattta cacaatatat
     3901 tttgtatagt aaaattattt tataatattt aaatccaatt gcacaaggag tgattatcat
     3961 ggtaccagaa gaaaaaggtt ctattacttt gtcaaaagaa gcagctatca tatttgcaat
     4021 cgcaaaattc aaaccattta agaacagaat taaaaataac ccacaaaaaa caaatccatt
     4081 tcttaaatta catgaaaaca aaaaatctta atcactttat ttatagcatt tctaatctca
     4141 gaaatgctat aaaggacaac gccctctttc ataactatat aagtcaagaa accaagacat
     4201 ttcaaacatc tttaacccct tcaatagcac attccctaaa acataaagca ataacaatga
     4261 acgatcaaaa ttccttacgt acttatgatg tgtcagtgaa tcgtctacac ttaattgaac
     4321 aaattctatg agaatagata ttgttaaatt aagaaagtag tcaattttat tatgacaaaa
     4381 gaaagaagaa catttagttc agagtttaag ttacaaatgg ttagattata taaaaatggt
     4441 aagcttaaga atgaaattat acgaaagtat gatttaaaac cctcaattat ctcaaattcg
     4501 ataaaacaac accaaaatac tggacccttc aatcatcaag ataatttaaa aagtgatgaa
     4561 aaagagttaa taaaattacg caaagaagtt caacatttaa aaatggaaca tgatgtttta
     4621 aagcaaattt taaagttgat ttagaaactg aactttttaa agatacatta cttgaagatt
     4681 ctaatttatc tgattatata aagaa
//
LOCUS       DANJFZ010000155         4695 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.156, whole genome shotgun sequence.
ACCESSION   DANJFZ010000155 DANJFZ010000000
VERSION     DANJFZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.156"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..265)
                     /locus_tag="PWN45_002550"
     CDS             complement(<1..265)
                     /locus_tag="PWN45_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624287.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(549..2357)
                     /locus_tag="PWN45_002551"
     CDS             complement(549..2357)
                     /locus_tag="PWN45_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL0624288.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            2474..2866
                     /locus_tag="PWN45_002552"
     CDS             2474..2866
                     /locus_tag="PWN45_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDL0624289.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2868..>4695
                     /locus_tag="PWN45_002553"
     CDS             2868..>4695
                     /locus_tag="PWN45_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0624290.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIY"
BASE COUNT         1694 a          640 c          804 g         1557 t
ORIGIN      
        1 aatcttttgt catgaaataa atgggatgaa tatcacgact agaagtaatg ttacgaacag
       61 gagcgtataa atctagagac gctaaattcg acatagtatg ttgctcaatt atggctgatg
      121 atgaatttaa agtatgtgcg ttggaactgt cggatttttg ttcataatgt tttgcatatt
      181 gcgccatgat gaatagtgta aaaataaata aaacaataag agatataatg cccataatca
      241 aaagtatttg tttagagcct ttcattattt cacatccttt ctaaaatata tttgtaacta
      301 aatttaaaat agttattttt gtaattctaa acctttttca tcgcgaaaac aattaaatag
      361 gtcgcggtat taattattat attattaccg cttaatatga aaaatacatg aaaattaatt
      421 ttctaatata cttttgaaaa attattacaa attagcccct tcaaaacgcg aaaacataag
      481 gattctagtt tcaaaagggc tgataagcat aaaatgaaat gtaatatttc gatgtataaa
      541 atttttaatt agctaaaaat catcgcatta attttttgag ctacatcatc aaaattcgga
      601 cattttaacg acacatataa tttaatttta ggttcagtac cagaaggacg taaagcgata
      661 aatccttcgt caaataagac acgaataaca tttgatttag gagaattaat ctgcgacgtt
      721 gtatctttat ccaaatgata aacctcgcta gttaaataat cttcaattgc tttcactttg
      781 agtccttgaa tctcttgcgg tggatttgaa cggaatttgg tcattattgc attaattttc
      841 tttttccctt caaatccttc tagcgtatgc gagaataatg tatcctcatg tctaccaaca
      901 gtttgataaa tctgttctaa ttcatctttc aatgttttgc catataattt taactcagaa
      961 gcgtatttta taatgagtgg cacaatttgt acggcatctt tatcacgtac aaaaggctct
     1021 gatagaaaac cgtaactctc ttcaaatgcg aaaatcatat tttgatgatc atccagttgt
     1081 cttatttcct gagcaataaa tttaaagccc gtcagcacct ctttgtattc aacattatta
     1141 tagcgtgcta atgactttgt taactcacta cttacaatag attgaatcat taagcggtga
     1201 cgtaattgag acgtttgttg aatacgataa ttaagtaaca gcgcaccaat ttggttgcca
     1261 ttaaaatatg tgatatgacc gtgtgcatca cgttcagcaa taccaagtcg atctgcatca
     1321 gggtcagttg atattaataa gtctgcatga cttttatttg caagttcgac tgcttgatca
     1381 aaagcgcgat gatcttcagg attagcactt tgaactgagc tgaaattcgg atcaggttta
     1441 cattgtgctt ctacaagatt aaattgattg aaattcaaag attgtaataa ctcaggtaca
     1501 atcggaacac ttgtaccatg caaactagtg aacacaactt gtaaatcaga tttaggaata
     1561 tagccaatca tattttgaat gtgtttcata tagtcatcag ttacagattt cggaaaaggc
     1621 ttgatataag atgtattttg tttagaaata ggtatatcaa tctgtaatgg atcgccaact
     1681 tcttcgatat aacgacttgc aagctcagat gcatcagtcg ataattgcgc accatcagaa
     1741 ccatatactt tgatgccgtt atagtctttc ggattatgac ttgctgtaat cataatgcca
     1801 gcagtagtat taagattgcg caccgcgaac gataattccg gtgtcgtttt atatgtatca
     1861 ggtaaataaa ctgttatttg atggtttgct aatacattag caattatttg ggcgaattca
     1921 gttgaaagat gtctaatatc ataatgaatg actattgttg gactgtttgt ttgggcattt
     1981 aaataacgcg ctaaacctaa tgccaatttt tcaatagtaa acttattaag tcgaccttca
     2041 ccaagaccga attttccgcg tatacccgca gtaccaaatg ttaatttact ttcaaaacct
     2101 tctcgctgtt caatgtcaga ttgctgctca taaaaatgtt taactaaact atcattagct
     2161 cgttctatcc ataattcttt atccattgtt gctaaacatc ctttcaaaat ctcagttaga
     2221 cttaataaaa catgaaaact aaagccctta catttatgta atgaattata aagaaatacg
     2281 ccccaaaagt aaaaaaacac agccccaaga caatactttt cacaagtatt atataataga
     2341 tgtgtatgaa aatgcatgga gtagagcgtt catgtgaatg taaaatatta atttttaata
     2401 ttgatttata tatagttttg taatttttga tagaataaca attaagatga ttttaaattg
     2461 aagggtgagt cttatgaaaa aagtaatcaa tgtagtagga gctattattt tttctgataa
     2521 caaaattctt tgtgcacaga gaagtgaaaa aatgagtctg cctttaatgt gggaatttcc
     2581 tggcggtaag gttgaaaaga atgaaactga aaaagacgct ttgattagag aaattagaga
     2641 agaaatgaaa tgtgatttaa ttgttggaga caaagttata actacagaac atgaatatga
     2701 ttttggaatt gttaggttaa caacatacaa atgtacttta aacaaagagt taccaactct
     2761 aactgaacat aagagtattg aatggttgtc aataaacgaa cttgataaat taaattgggc
     2821 cccagcggat ataccagccg ttaataaaat tatgactgag ggataatatg agtagattac
     2881 taaatgattt caatcaatct ttacataaag ggtttataga caaacatatt tctcataaag
     2941 ggaattatac accgaagctt ttagtaaata ataaaaatga aaaagttctt tcaacaatta
     3001 tagatgaact tcaaaaatgt gaaacgtttt atttttctgt ggcttttata acagaaagcg
     3061 gtttagccag cttaaaggct caactattag atttaagcaa taagggtgtt aaagggaaaa
     3121 tattaacatc taattactta gggtttaata gtcctaaaat gtatggagaa ttacttaaat
     3181 taaaaaatgt agaggtaaga ttaactgata ttgctggatt ccatgccaaa ggatatattt
     3241 ttgagcataa ggattatagt tctatggtaa taggaagctc taatttaaca tctaatgcgt
     3301 taaaggttaa ttatgagcat aatgttttac tgtctactat gaaaaatggc gatttagttg
     3361 atagtgtaaa aagtgaattt gacttgttat ggcaaaagag taccccactg actgagcaat
     3421 ggattaattc atataaagaa tcatttgagt accgttcctt agaaaaatta gcggaggttg
     3481 aacaaactca aatgctgcta gctgataagg tgaaaaaatc agtggaaatt gtacccaatt
     3541 taatgcaagc agaagcatta aggtcattaa aagctattag ggataaagcc aaagataagg
     3601 cgttaataat atctgcaact ggtacaggta aaacgatttt atgtgcatta gatgttagag
     3661 aagtaaaccc taataaattt ttatttattg ttcataatga ggggatttta aatagagcta
     3721 aggaagaatt taaaaaagta ttacctataa aaaatgatag tgattttgga ttgttgacag
     3781 gaaaacatag agatgtcgat gccaaatatt tatttgcaac cattcaaaca ttatctagag
     3841 atgataattt taagcaattt gatgaaaatg aatttgatta tattgttttt gatgaagcac
     3901 atcgttctgc tgcatctact tatcaacgag tatttaacta cttcaaacct aagttcatgc
     3961 tgggaatgac tgctacacca gaaagatcag atgaattaag tatatttgaa ctgtttgatt
     4021 ataatattgc atatgaaata aggttacaag cagcattaga aagtgatatt ttatgtccat
     4081 ttcattattt tggtgtgact gattatgtac atcaaggtat taaagaagat gatgtaacta
     4141 aactgagata tttaacttct gatgaaagag ttaattatat tattcaaaag acagattact
     4201 atggatattc aggtgaaatt ttacaaggat tgatttttgt aagtagcaaa aaagaagctt
     4261 atgatttagc ggataaacta agttcaaaag ggattaaaag tgttgcttta acaggagatg
     4321 atagtgttaa ttatcgccaa attgtaatag aaaaattaaa agaaggtaag attaactata
     4381 ttataactgt agatcttttt aacgaaggta ttgatattcc agaagtcaat caagttgtaa
     4441 tgttgagacc aacagagtct agtattatat tcattcagca acttggtaga ggattaagaa
     4501 aaagttcaaa taaagagtat gttacagtaa ttgattttat tggtaattat aagacaaatt
     4561 atttaattcc gattgcgctt tctggggatc aatcgcaaaa taaagataat tataagaagt
     4621 ttttaacgaa taacgattcg attaatggag tatctacaat taattttgaa gaagttgcta
     4681 aaaaacagat ttaca
//
LOCUS       DANJFZ010000156         4689 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.157, whole genome shotgun sequence.
ACCESSION   DANJFZ010000156 DANJFZ010000000
VERSION     DANJFZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.157"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            81..683
                     /locus_tag="PWN45_002554"
     CDS             81..683
                     /locus_tag="PWN45_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624291.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            950..4102
                     /locus_tag="PWN45_002555"
     CDS             950..4102
                     /locus_tag="PWN45_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDL0624292.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4159..4641
                     /locus_tag="PWN45_002556"
     CDS             4159..4641
                     /locus_tag="PWN45_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624293.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         1795 a          582 c          862 g         1450 t
ORIGIN      
        1 taaacaagta actttacaaa aattagaatg attcaattca atcataattg tttgaaaata
       61 gaggtgagca ggtgaacgat atgttaatta gtcttgtaat cccagttttt gctttgttag
      121 ttattggtgg tattatttgg atgattatag aaggtatagt acatatttca aaaaagaata
      181 aagcaattga taactttttt aaccaagtta ataaagtaag tgagacatat aaattcgcta
      241 ctactttttt atttctaatc ttggctacgg ctggtatttc tcaattttat ctatattata
      301 tagtatcagc gtttcttttt tggcttgtca tacttacctt tggcattgca ggtatcattt
      361 ttttaatgcc atatggatta tgttttctac cgttttataa gcaaaaaaag aaaaaacaga
      421 catttaaaaa atacatggtt tacactacga ttggtttgtc aatttgtcta ggcttatctc
      481 tagttttggt tcacactacg aaaatttata tggacgaagg tggcgtaaga tactattacg
      541 gtagttttgt aatgaaacaa gcgggcggtt atgcttattt agctttagcg gtactttcaa
      601 cgttgttaat tgttgcgaaa aaagctacaa ataaaaataa agaaatcgaa accgtcgaca
      661 atacaaatat aacggaaaga taattaaggg agtgctcatt caggagtgct cttttttgat
      721 gtccaaattt agttgcaaat gaaggcatag aagaaatttt tatagtcatt tattggataa
      781 tgaattatgg tcatatcgat taattttatt agtgaggatt ttacaaacat ttgttgaata
      841 aatatggtaa tgataatata tgaatatttt tgaaaaaaca ctaatatgat taaattattt
      901 gttaaaatag cattaagtaa aaaacaaata gggaacaagg tgggatttca tgagtcaatt
      961 gctaaatgat acgttatcgg cttggttgtt aattgaatct ttaagtccag gagaagtaaa
     1021 ttttacagcg gaagatatac tctcagctga acattttaaa aatggtgcaa agcaagcaca
     1081 acttcaaagt tttgatgaat attttgaaat atggaatgct gaacgcttta ttatatcaga
     1141 agaaaaatca gagactgggg aacttatatt taaattttat agacattgct tccgctataa
     1201 tgaaattaat ttgaaaattc aggatatttt tgatgattat tctgagattc ataatccaaa
     1261 tgggacacac tgttatggtt acacatttaa cacagataaa cacggcaaag tgatagttga
     1321 ttctatacat attccgatga ttatgagtgc attaaaagaa attgaaaaga acaaaaatgc
     1381 caatatagaa gaaaaattta atgattctgt tgaaaaattt gttcaaaaag taaaagaaat
     1441 tttagcagat gaaccaatta atgaatttaa attgaagaag atggacaaag cttatgatga
     1501 gtacttttct gtattaaatt caaagaaaga tggattattt ggacattatg tagcaataga
     1561 atatgtgaaa gatagtgatt taccacagcc ggaatttaac agcttcttca taagtgatat
     1621 tgagaaagca agaaaatctc ccaaccaaac tttaattgat tacattgaag gtgtagaaga
     1681 aagtcagcac atagaagtag atgaaaataa agaaatgttt gacaaatttt tacatccttc
     1741 acgtttgcct gatggacgat ggccatcaca gactgagttt agattgtctt taatgcaaca
     1801 acttgctgta aaccaaatta cgagtggtaa tgaaagaata agttcagtta atgggccacc
     1861 aggtacaggt aagactactt tattaaaaga tatatttgct catctagtag ttgaaagagg
     1921 taaagagtta gctaaactaa ataatcctaa agatgcattt gtcaaaacaa aaattcatga
     1981 aacggatgat aaatacgtat acttactaaa ggaatctatt gccaaatata agatggtagt
     2041 cgcatctagt aataatggag ctgttgaaaa tatatctaaa gatttaccga aaattgaaga
     2101 aattataaga aatcccgaaa aatgtaaatt ccctaaatat gaacagaatt atgcaaattt
     2161 agcacatgaa ttaaaagatt ttgctgaaat agctgaagat ttgattggtg aaagtgcctg
     2221 gggcttattt tctggagttt ttggtaaaag tactaatatt aaccaagtat tgagtcatat
     2281 gttaaaacaa gatgcgaatg atattggctt tgctaaatta ctacaaaatg agaataatcg
     2341 tatgagttat aacgagttaa tgagtgaatg gcaatcacat caacgtgcat ttttagaaga
     2401 gttgaggcat gttgaaatgt taaaagaaga atctattaga gcatatgatg tttataaaaa
     2461 ttgtgagtct ttctctaaga ttgaacaggt tattaatagt gaaaaaacaa gtattgaaga
     2521 acaggtatat catttagata atgaaacgtt acgagacaat aaagaaatag aagatttgga
     2581 taatcgaatt aattatattg ttaagcaaat agaaacttta aatgagttaa ttaaatccat
     2641 caaagaaagc aacaaaggtt ttattaacaa actgaaagcg atgtttaatt cagaagaaga
     2701 tgaaagctat aaagatcata ataaagagaa gcaacaatta ttaacacaac agttagagtt
     2761 agagaaatgt aaaaaaaaca aacatgaaga ccttgttagc aaactaaaag aaaaagagaa
     2821 attaattaaa caattaacta aagtacagtt gcaattagac gagttaaatt cacagttaca
     2881 agagttagaa gcatatcgta ttgagtcaaa aattacaatt ccagaaaaag atttttggag
     2941 tgacaacaat tatgatgagc gccaagttac taatctgtgg acgagtgacg aacttcaata
     3001 cagacgtgcc atgctctttt taagagcaat gatattgcat aaattattat tgattgctaa
     3061 taatacaact atttattatg cgattaatga ttttaaagat agaaggaaat taattgatgc
     3121 aaatccagat aaagtacaca acgcatggaa tgtgatgcat ttaatatttc cagtagttag
     3181 tacgacgttt gcaagcttta aatctatgta tgggggcata ccaaaagatt tcatagacta
     3241 cttatttatt gatgaagcag gacaagcaat acctcaagca gctgtgggag cattatatcg
     3301 ttcaaaaaaa gttgtagctg taggtgatcc gattcaaata gaaccggttg tgactttaga
     3361 aagtcattta attgataaca ttcgtaaaaa ttatcatgtt ccggaatatc tagtttctaa
     3421 agaagcttct gtgcagtctg ttgcagacaa cgccaatcaa tatggttttt ggaaatctga
     3481 tgctactgat agtaatcaaa aaacctggat aggcatacct ttatgggtgc acagacgatg
     3541 tttaaaacct atgttcacga tagctaacca aatcgcttat aataataaaa tggtgttgcc
     3601 aagtaatatt acaaaagtag gtaaaacagg ttggtatgac gttaaaggaa acgcagttca
     3661 aaaacaattt gtgaaagagc atggtgaaaa agtagtggga ttattagctg atgattggat
     3721 tgaagcaatt aaggaaggta aaaatgaacc gagctcattt gtaatatcgc ctttttcagc
     3781 agtacagcaa cagattaaac gtatgttaaa gcaacaacta ccgactagaa ttgatattga
     3841 acgtacaaaa attaatcaat gggtcgataa atccattggt actgttcata cttttcaagg
     3901 taaagaggct cagaaggtgt attttgtaat aggtactgat aatacccaag atggtgctgt
     3961 gaactggtca tgcgaaaaac caaacttgtt aaacgttgca gtgacaagag ctaagaaaga
     4021 gttttatgta attggcgaca tgcaaagaat acagatgaaa ccattttatg agacgatttt
     4081 taaagaaaga aatgtaaaat aacatacaaa aaagtatata gaggaagtta tacattttaa
     4141 aaggagcaaa attgaataat ggagaaattt aacaactgga tattaaatgc aataagtgga
     4201 tctcaaacag acaagaatga aacaactgaa gaattaaaag gggcaaaatt tatcatttta
     4261 tatgcatatt caatgctcgt tttgcttgcg ttagtaattt ctaacatatt cattcacatt
     4321 ttggagccta aactatcaat caccactcaa atcatcatcg ttttgatttt aattgaagca
     4381 ctaattggac tgcgtttctt gaaagcgtac gatgttaagc gtggcaaaga taaagaaaat
     4441 aagaaaaata gtaaggattt cgttaaacta aaatcaattt tagtagcaat tttatttaca
     4501 tcattggcgc tgacagcagg tactgtagct gatatatacg gtttcactga cttaggaaat
     4561 actagaagtg atttaatcgt ttggagcata ggtggtatta tatttggcct cgtatgttac
     4621 acaatggaag ataaaagata acgataagga gctggcgatt ataaagctag ctcctttttt
     4681 aacttatat
//
LOCUS       DANJFZ010000157         4683 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.158, whole genome shotgun sequence.
ACCESSION   DANJFZ010000157 DANJFZ010000000
VERSION     DANJFZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.158"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            299..685
                     /locus_tag="PWN45_002557"
     CDS             299..685
                     /locus_tag="PWN45_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL0624294.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            678..974
                     /locus_tag="PWN45_002558"
     CDS             678..974
                     /locus_tag="PWN45_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0624295.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      1032..1135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1224..2249
                     /locus_tag="PWN45_002559"
     CDS             1224..2249
                     /locus_tag="PWN45_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDL0624296.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2242..2937
                     /locus_tag="PWN45_002560"
     CDS             2242..2937
                     /locus_tag="PWN45_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624297.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            2955..3776
                     /locus_tag="PWN45_002561"
     CDS             2955..3776
                     /locus_tag="PWN45_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0624298.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(3919..>4683)
                     /locus_tag="PWN45_002562"
     CDS             complement(3919..>4683)
                     /locus_tag="PWN45_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0624299.1"
                     /translation="TRRIYKLQDLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESI
                     IKDINTKAQKMHSGIHKRFYLFVALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNIN
                     GTIHWFHDESGGFGVKDTTKTESSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNR
                     NFVFTNHKGNPMQTERFNKILREAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIM
                     DRVGHSDHRTTLSIYSHVTEQMDKDMMNKLEQVKLG"
BASE COUNT         1616 a          668 c          845 g         1554 t
ORIGIN      
        1 attattgtaa gctgacttac catcagcttc tgtgttgggg ccccgctata attgaaaagt
       61 ttgttatagg tgtattttct tttggttaac tattgttaat ataacattgt agattttagg
      121 atgttgattt ttgacttagc ctttgtctgc ttatgttaaa tgttccagct agtcaaacat
      181 tgagtttgta attattatat taagctagtt taaatttttt gtgatttatg ttttattcat
      241 accaattttg tgaaatgata cagtgacaga tgtttattga ttttaaaggg tgatgatgat
      301 ggctattgta aataaagtga taattgttga aggaaaatct gataaaaaaa gggtgcaaca
      361 ggttattgca gaaccagtca atattatttg tactcatgga acaatgagta tagataagct
      421 tgatgatatg atagaatcac tgtatgataa acaagttttt gtattagccg attctgatga
      481 cgaaggagat cgaattagaa attggtttaa acgttatttg agtgaaagtg aacatatatt
      541 tattgataaa acttactgtc aagttgcgaa ttgccccaaa caatatttgg cgcatgtact
      601 ttcaaaacat ggctttactt gtaagaaaga aacacctctt ttaccgaata taaataatga
      661 aaggttagtt ttagtaaatg aataattcat tagacatcaa agatgtaact acattttatg
      721 aggaagacaa acatttaatc tttggttata caccaacgtg tggtacttgt aaggtttcag
      781 aaagaatgtt agacattgct aatgaaatat tgcagttacc attattgaaa atagatttaa
      841 acttttatcc tcagttttgt aaagatatgc aaatcatgtc tacgccgatt ttattgttga
      901 tgaataaaga taaagaagta aaacgaattt atgcatttaa atcggtgact gatttgttag
      961 aaaatttaaa atagttattg acgaaatttt actaaggtgt taggattaat acaaattaaa
     1021 tgatgcgtaa actcttatcg agagtggtgg agggatgtgc cctacgaagc ccggcaaccg
     1081 tcttatatag aaatggtgcc aattcacata aagttttaac ttttgaagat gagagaaaca
     1141 atactactat tgctttctca atttttctat cgatattgag aaagcatttt ttattttatt
     1201 aagcaacaca gggaggaatc aacgtgattg aattaaaaga agttgttaaa gaatatcgga
     1261 ctaaaaataa agaagtcctt gctgtagatc acgttaattt atcgattcga gcaggatcga
     1321 tttatggcgt cattggtttt tctggagcag gaaaaagtac tttgattcga atgtttaatc
     1381 atttagaagc gcctacatca ggtgaagtta ttatagatgg agaccatata ggtcaattgt
     1441 ccaaaaatgg attaagagca aaaagacaaa aagtaagtat gatcttccaa cattttaatt
     1501 tgttatggtc aaggactgtg ttaaaaaata ttatgtttcc gcttgaaatt gcaggtgtcc
     1561 ctagaaggag agctaagcaa aaagcattag aacttgtcga actcgtcggt ttaaaaggta
     1621 gagaaaaggc ttatccatca gagttatcag gtggacaaaa gcaacgtgtt gggattgcac
     1681 gagcgttagc taatgatcca acggtcttgc tttgtgatga ggcaacaagt gcacttgatc
     1741 cgcaaacaac agatgaaatt ttagatctac tactaaaaat tagagaacaa caaaatttaa
     1801 caattgtact aattacgcat gaaatgcatg tcattcgtcg tatttgtgat gaagttgcag
     1861 ttatggaaag tggtaaagtg atagaacaag gaccggtgac acaggttttt gaaaatccgc
     1921 aacacactgt gacaaaacga tttgtgaaag acgatttaaa tgatgatttc gaaacatctt
     1981 taacagaatt agagccatta gaaaaagatg catatatcgt tagattagtt ttcgctggtt
     2041 caacaacaac cgagcctatt gtatcgagtc tatcaactgc ctatgatatt aaaattaata
     2101 ttttagaagc aaatattaaa aatacaaaaa atggaacagt cggcttttta gttctgcata
     2161 ttccatatat ttcaagtgta gatttcggaa aattcgaaaa agagttaatt gagcgacaag
     2221 ttaaaatgga ggtgttaaga catgggtaaa tcatttagtg aaattataaa tgaaatgatt
     2281 acaatgccta atattcagtg gccagaagtt tggactgcaa tagtcgaaac actatacatg
     2341 acagtcgtct caactatatt tgcatttata cttggtctta ttttaggtgt gttattattc
     2401 ttgtctgcta aaggtaagtc tatcggtgca aggttatttt attctatcgt ttctttcatt
     2461 gttaacttat ttagagcgat accatttatt attttaattt tattattaat tccatttaca
     2521 agtttgatac ttggaacgat aagtggtccg acaggtgcgt taccagcctt gatcattggc
     2581 gcagcaccgt tttatgcaag gctcgtagaa attgctttta aagaaattga taaaggtgtc
     2641 atcgaagcgg cttggtcaat gggcgctaat acttggacag taattcgtaa agtcctttta
     2701 cctgaagcta tgccagcgct agtgtctggc attacagtta cagcaatcgc tttagttggt
     2761 tcaacagcag ttgcaggtgt aattggtgcc ggtggtttag gaaatttagc atacttaaca
     2821 ggtttcactc gaaatcaaaa tgatgtcatt ttagtatcaa cagtttttat tttaattatt
     2881 gtatttataa tccaattcat tggggattgg cttacaaata aacttgataa acgataaatt
     2941 gggggtttca ttttatgaaa aaattatttg gtcttatttt agtattaaca tttgcagttg
     3001 tattagcagc ttgcggtaat ggaaacaaaa gtggcagtga cgacaagaaa ataacagtag
     3061 gtgcttcacc agcaccacat gctgaaattt tagaaaaagc aaaaccatta ttagagaaaa
     3121 aaggttatga actagatatt aaaacaatta acgattacac tacacctaat aaattactag
     3181 acaaaggtga aattgacgca aactatttcc aacatacacc atatttaaac acagagaaaa
     3241 aggataaagg ttacaaaatc gtaagtgccg gtgatgttca cttagaacct atggctgtat
     3301 actctaaaaa gtataaaagt ttaaaagaat taccaaaagg tgcaacagtc tatgtgtcta
     3361 ataatccagc tgaacaagga cgtttcttaa aattcttcgt tgatgcaggt ttaattaaaa
     3421 tcaaaaaagg cgtaaaaatt gaagatgcta agtttagtga tattacagag aataaaaaag
     3481 atattaagtt taataataaa caatcagcag aattcttacc taaaatttat caaaatgaag
     3541 acgctgatgc tgttatcatt aattcgaact ttgcaatcga acaaaaacta aatcctaaaa
     3601 aagattctat tgctgtagaa agtgcgaaag ataatcctta tgcaaactta attgctgtta
     3661 aagaaggaca tcaagatgat aagaaaatca aagcattaat tgaagtatta caatctaaag
     3721 atattcaaga cttcattaat gaaaaataca atggtgcagt tattcctgct aaataatacc
     3781 aattaaataa aaataccctt taataacaac gattaagggg ttatttttgt ggtattgaaa
     3841 ataaaaaagg gcacaaaagg ggcagtatat gcaaattatc gaattttaaa caaaaaaata
     3901 cgctcagatt tgagcgcatt atccaagttt tacctgttca agtttgttca tcatatcctt
     3961 atccatctgt tcagtaacgt gagaataaat ggaaagtgtt gtacggtggt cggaatggcc
     4021 tacacgatcc ataatagctt taagtgacac gccttgttga gagagtaatg atatgtggct
     4081 atgtcttaat atatgtgaag aaacttcttt atcaatacca acatctttag ctgcttccct
     4141 aaggatttta ttgaatcttt cagtctgcat tgggttgcct ttatgattag taaatacaaa
     4201 atttctattt agatatccat cattccattt tgaatccttt ttgttctcca gtattgcttt
     4261 ctttaatatc tcgcaacttc tgctactcaa tccaattgtt ctataactag actctgtttt
     4321 agtggtatct tttacaccga atccaccaga ttcatcgtga aaccaatgga ttgttccgtt
     4381 aatatttaaa ctcttattat caaagtctat atcttcattt tgaatggcta acatttcacc
     4441 aatacgcata ccatttaagg cttggaattc tgtcatgagt gcaacgaata aataaaaccg
     4501 cttatggata cctgagtgca tcttctgagc cttcgtatta atatctttga tgatagattc
     4561 tatttcattt aattcgaggt acttattacg tttagctttc acttcatcat aagacttaat
     4621 tcttttattt aatgttatat cttttaaaaa ttctaaatct tgcagtttat agatacgtct
     4681 agt
//
LOCUS       DANJFZ010000158         4675 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.159, whole genome shotgun sequence.
ACCESSION   DANJFZ010000158 DANJFZ010000000
VERSION     DANJFZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.159"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..173)
                     /locus_tag="PWN45_002563"
     CDS             complement(<1..173)
                     /locus_tag="PWN45_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0624300.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
     gene            227..598
                     /locus_tag="PWN45_002564"
                     /pseudo
     CDS             227..598
                     /locus_tag="PWN45_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            1435..2178
                     /locus_tag="PWN45_002565"
     CDS             1435..2178
                     /locus_tag="PWN45_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDL0624301.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2275..2703
                     /locus_tag="PWN45_002566"
     CDS             2275..2703
                     /locus_tag="PWN45_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDL0624302.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2749..>4675)
                     /gene="mecA"
                     /locus_tag="PWN45_002567"
     CDS             complement(2749..>4675)
                     /gene="mecA"
                     /locus_tag="PWN45_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDL0624303.1"
                     /translation="KEINNTIDAIEDKNFKQVYKDSSYISKSDNGEVEMTERPIKIYN
                     SLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTNYGNIDRNVQFNFVKEDGMWKLDWDH
                     SVIIPGMQKDQSIHIENLKSERGKILDRNNVELANTGTAYEIGIVPKNVSKKDYKAIA
                     KELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMDEYLSDFAKKFHLTTNETESRNYPL
                     GKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGKKGLEKLYDKKLQHEDGYRVTIVD
                     DNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNMKNDYGSGTAIHPQTGELLALV
                     STPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITTSPGSTQKILTAMIGLNNKTL
                     DDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQAIESSDNIFFARVALELGSK
                     KFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNEILLADSGYGQGEILINPVQILSIY
                     SALENNGNINAPHLLKDTKNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYAN
                     LIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMAINVKDVQDKGMASYNAKISGK
                     VYDELYENGNKKYDIDE"
BASE COUNT         1569 a          707 c          770 g         1629 t
ORIGIN      
        1 aaaattgggg catattcttg aacccaacgg tagaccgttg aatgatgaac gtttacacca
       61 cgttccctta atatttcaga tatatcacga taactcaatg tatatcttag atagtagcca
      121 acggctacag tgataacatc cttgttaaat tgtttatatc tgaaatagtt catacagaag
      181 actccttttt gttaaaatta tactataaat tcaactttgc aacagaaccg tattatggaa
      241 tagagatgtt ggtaacattt atacaggatc attatactta agtttaattt cgttattaca
      301 gaaccacaca ttccaaccag aagagaaagt atgtctattt agttatggtt caggagcagt
      361 aggagaaatc tttagtggtt caatcgttaa aggatatgac aaagcattag ataaagagaa
      421 acacttaaat atgctagaat ctagagagca attatcagtc gaagaatacg aaacattctt
      481 taacagattt gataatcaag aatttgattt cgaacgtgaa ttgacacaag atccatattc
      541 aaaagtatac ttatacagta tagaagacca tatcagaaca tataagatag agaaataaac
      601 tagtggccga ttgtgcttga tgagcttggg acataaatcc taactcgaaa taaataagca
      661 tatcactaaa ctgatttttt aaagtttaca gtgatatgct tattttttta tcttacgatt
      721 ttgtacgtgc atgcttgcct aggggtatgg ctcgagccat tagtctctcg cacatactat
      781 tccctcaggc gtcagcactt acaaaatcgg ttgtaatttt catttttata cgcattctta
      841 ctgagattat actaataaga ggaatagtaa aagcaattct aagtaaaatt gcagataaga
      901 ggtttgttaa aagcagttct cagtaaaatt acagataaga ggtacgttaa aagcagttct
      961 aagtaaaatt gcagataaga ggtttgttaa aagcagttct aagtaaaatt gcagataaga
     1021 ggtacgttaa aagcaattcc atgcaaaatt gctgataagg ggtaagttaa aagcagttct
     1081 cagtaaaatt gcagataaga ggtacgttaa aagcagttct aggcaaaatt gcagataaga
     1141 ggtgcgttaa aagcagttct cagtaaaatt gctgataagg ggtaagttaa aagcaatcct
     1201 aagtaaaatt gcagataagg ggtacagaaa aactagactt gattacaaaa tggagcttgg
     1261 gacataaatg attttttaaa aatgagatga gacgtagatt aactccataa tcaatacgaa
     1321 tctatcgact tctttattta tgatattcat ctctttttaa tggaaataaa agtgcgatta
     1381 atgtgataat acagttacgt taattaaaaa aataaaaatg caaggagagg taatatgcta
     1441 actgtatatg gacatagagg attacctagt aaagctccgg aaaatacaat tgcatcattt
     1501 aaagctgctt cagaagtaga aggtataaac tggttggagt tagatgttgc aattacaaaa
     1561 gatgaacaac tgattatcat tcatgatgat tatttagaac ggactacaaa tatgtccggg
     1621 gaaataactg aattgaatta tgatgaaatt aaagatgctt ctgcaggatc ttggtttggt
     1681 gaaaaattca aagatgaaca tttgccaact ttcgatgatg tagtaaaaat agcaaatgaa
     1741 tataatatga atttaaatgt agaattaaaa ggtattactg gaccgaatgg actagcactt
     1801 tctaaaagta tggttaagca agtggaagaa caattaacaa acttaaatca gaatcaagaa
     1861 gtgctcattt caagctttaa tgttgtgctt gttaaacttg cagaagaaat catgccacaa
     1921 tataacagag cagttatatt ccatacaact tcgtttcgtg aagactggag aacactttta
     1981 gattactgta atgctaaaat agtaaacact gaagatgcca aacttactaa agcaaaagta
     2041 aaaatggtaa aagaagcggg ttatgaattg aacgtatgga ctgtaaacaa accagcacgt
     2101 gcaaaccaac ttgctaattg gggagttgat ggtatcttta cagacaatgc agataaaatg
     2161 gtgcatttgt ctcaatagaa agttagaggt gagtcttacg tttcagtgac ggtagactta
     2221 cctttaacat gttacatact aaaaaattaa tttgaataag aaagagagac atatatgaaa
     2281 tacgatgatt ttatagtagg agaaacattc aaaacaaaaa gccttcatat tacagaagaa
     2341 gaaattatcc aatttgcaac aacttttgat cctcaatata tgcatataga taaagaaaaa
     2401 gcagaacaaa gtagatttaa aggtatcatt gcatctggca tgcatacact ttcaatatca
     2461 tttaaattat gggtagaaga aggtaaatac ggagaagaag ttgtagcagg aacacaaatg
     2521 aataacgtta aatttattaa acctgtatac ccaggtaata cattgtacgt tatcgctgaa
     2581 attacaaata agaaatccat aaaaaaagaa aatggactcg ttacagtgtc actttcaaca
     2641 tacaatgaaa atgaagaaat tgtatttaag ggagaagtaa cagcacttat taataattca
     2701 taataaaaca gtgaagcaac catcgttacg gattgcttca ctgttttgtt attcatctat
     2761 atcgtatttt ttattaccgt tctcatatag ctcatcatac actttacctg agattttggc
     2821 attgtagcta gccattcctt tatcttgtac atctttaaca ttaatagcca tcatcatgtt
     2881 tggattatct ttatcatatg atataaacca cccaatttgt ctgccagttt ctccttgttt
     2941 cattttgagt tctgcagtac cggatttgcc aattaagttt gcataagatc tataaatatc
     3001 ttctttatgt gttttattta cgacttgttg cataccatca gttaatagat tgatattttc
     3061 tttggaaata atatttttct tccaaacttt gtttttcgtg tcttttaata agtgaggtgc
     3121 gttaatattg ccattatttt ctaatgcgct atagattgaa aggatctgta ctgggttaat
     3181 cagtatttca ccttgtccgt aacctgaatc agctaataat atttcattat ctaaattttt
     3241 gtttgaaatt tgagcattat aaaatggata atcacttggt atatcttcac caacacctag
     3301 ttttttcatg cctttttcaa atttcttact gcctaattcg agtgctactc tagcaaagaa
     3361 aatgttatct gatgattcta ttgcttgttt taagtcgata ttaccattta ccacttcata
     3421 tcttgtaacg ttgtaaccac cccaagattt atctttttgc caacctttac catcgatttt
     3481 ataacttgtt ttatcgtcta atgttttgtt atttaaccca atcattgctg ttaatatttt
     3541 ttgagttgaa cctggtgaag ttgtaatctg gaacttgttg agcagaggtt cttttttatc
     3601 ttcggttaat ttattatatt cttcgttact catgccatac ataaatggat agacgtcata
     3661 tgaaggtgtg cttacaagtg ctaataattc acctgtttga gggtggatag cagtacctga
     3721 gccataatca tttttcatgt tgttataaat actcttttga actttagcat caatagttag
     3781 ttgaatatct ttgccatctt ttttcttttt ctctattaat gtatgtgcga ttgtattgct
     3841 attatcgtca acgattgtga cacgatagcc atcttcatgt tggagctttt tatcgtaaag
     3901 tttttcgagt ccctttttac caataactgc atcatcttta tagcctttat attctttttg
     3961 ttttaattct tcagagttaa tgggaccaac ataacctaat agatgtgaag tcgcttttcc
     4021 tagaggatag ttacgacttt ctgtttcatt agttgtaaga tgaaattttt ttgcgaaatc
     4081 acttaaatat tcatccattt ttttaacggt tttaagtgga acgaaggtat catcttgtac
     4141 ccaattttga tccatttgtt gtttgatata gtcttcagaa atacttagtt ctttagcgat
     4201 tgctttataa tcttttttag atacattctt tggaacgatg cctatctcat atgctgttcc
     4261 tgtattggcc aattccacat tgtttcggtc taaaatttta ccacgttctg attttaaatt
     4321 ttcaatatgt atgctttggt ctttctgcat tcctggaata atgacgctat gatcccaatc
     4381 taacttccac ataccatctt ctttaacaaa attaaattga acgttgcgat caatgttacc
     4441 gtagtttgtt ttaattttat attgagcatc tactcgtttt ttatttttag atactttttt
     4501 tattttacga tcctgaatgt ttatatcttt aacgcctaaa ctattatata tttttatcgg
     4561 acgttcagtc atttctactt caccattatc gcttttagaa atataactgc tatctttata
     4621 aacttgtttg aaatttttat cttcaattgc atcaatagta ttattaattt cttta
//
LOCUS       DANJFZ010000159         4623 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.160, whole genome shotgun sequence.
ACCESSION   DANJFZ010000159 DANJFZ010000000
VERSION     DANJFZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.160"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..265
                     /locus_tag="PWN45_002568"
     CDS             <1..265
                     /locus_tag="PWN45_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDL0624304.1"
                     /translation="TLLKLPKVTKRWGGKTKTHFGLLLTALSSFIYIEKIIEYILVPN
                     VVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYIFELNTEDDD"
     gene            412..1023
                     /locus_tag="PWN45_002569"
     CDS             412..1023
                     /locus_tag="PWN45_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDL0624305.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            1159..1377
                     /locus_tag="PWN45_002570"
     CDS             1159..1377
                     /locus_tag="PWN45_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624306.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1509..2009
                     /locus_tag="PWN45_002571"
     CDS             1509..2009
                     /locus_tag="PWN45_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624307.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFTREGELKVSFDYIDWINSEFGQVGRQNYYKYRKFGILPETEYEINKVKEIEQYVKE
                     QEEAEQ"
     gene            2020..2520
                     /locus_tag="PWN45_002572"
     CDS             2020..2520
                     /locus_tag="PWN45_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624308.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            2531..3026
                     /locus_tag="PWN45_002573"
                     /pseudo
     CDS             2531..3026
                     /locus_tag="PWN45_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            3037..3528
                     /locus_tag="PWN45_002574"
     CDS             3037..3528
                     /locus_tag="PWN45_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624309.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            3738..4421
                     /locus_tag="PWN45_002575"
     CDS             3738..4421
                     /locus_tag="PWN45_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDL0624310.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIMQYSRFYI
                     FELNTEDDD"
BASE COUNT         1768 a          457 c          836 g         1562 t
ORIGIN      
        1 cacgttatta aaattaccta aagtaacaaa acgttggggt ggtaaaacta aaacacattt
       61 cggtttatta ttaactgcgt tgagctcatt tatatatatt gaaaaaatta tcgaatatat
      121 attggttcct aatgtcgtag aatctaagtt tataattatt gtgagcatgt tggttatcgc
      181 tggagcacaa tttgtggcat ttcaatttat tatgcaatac agtagattct atatttttga
      241 attaaacact gaagatgatg actaaacaat tttataaatt ctgtgaagta tacttatata
      301 taagataatc aacttcaacg tatatcacca tattatttta tactcaatga tgtgaatgga
      361 tttaagtgga ttgatattaa agtaaaagtt atggaatatt gaggtgtgaa tatggagttc
      421 ttattattaa ttgtcgtagc cggactgtat tatattatat atttaactgc tgtgatgtat
      481 tctgaaaaaa tagtagtatt gcctataatc atctatgcca ttgtgtttgt aataattggt
      541 atcacttata tctttatagg cgacagctat gatcaattaa caaatttcaa tgtgattttg
      601 tatatgggga gtttgtttta tgcatggatg gctattagaa atctttggaa cagaccacta
      661 ttactaaaat ataagaacat tacagatagt tcaagtggaa tagttaataa atctgaatac
      721 aattcagttg aaagcttacg tataaatatt gaaatagcta agtataaagg gattatttct
      781 ttgatagtag ctatagtact aacggtatta atgacattaa agtcaacacc tcaaatcact
      841 gcagaaacac gtgacttaag tatctcattt ttcatactca gcttatttat cattattata
      901 tttgcagttt gggatttgtt tattagagtt agaaaaggag cgtttgcttt tgttgtaata
      961 aggccaatat tatttagttg ttggttattt attttgaata tgattttatc aagattatta
     1021 taattgtatt acatataggt gtgggtaaat ttttatttat gaaatgataa aaaagtatat
     1081 actattaata tcttttttac tataattata aatagttaaa taaattgaaa gacaaaataa
     1141 tgagtaggag ttaatgagat ggaaaaccaa aaacaaggca atggcttaaa aattgcaaca
     1201 tgggtattta ttgtattaac agtagttaca ccgctatttg gtattggaag tattgtttgt
     1261 agtattaatt ataaaaaata cgatgctgaa aaaggttcga agttattgca aattgcaatt
     1321 atcgtaacaa taattgcttt tgttttaaat ttattagcat atttaggttt aagataagta
     1381 aaacgaattt gaagaagcat taagcgacat ttgggtgttg tttaatgctt tttatttaac
     1441 ggaaataagt gtagagaata aattaatagt ttaatcaaga gatatttcga acacataggg
     1501 gcgataacat gactttcgaa gaaaaattaa gtgaaatgta tagcgagatt gcgaataaga
     1561 ttagcagcat gataccggta gagtgggaaa aggtatatgc aatggcatat atagatgacg
     1621 gaggaggaga agtgttctac tattacacag aacctggaag taatgaatta tactactata
     1681 ctagtgtatt aaataaatat gatatatcgg aatcggaatt tatggattca gtgtatgagt
     1741 tgtataaaca atttcaaaat ttaaggaatt tatttaaaga agaaggacat gaaccatgga
     1801 catcatgcga atttgacttt acaagagaag gtgaattaaa agtttcattt gattatattg
     1861 attggataaa ttcagaattt ggtcaagtag gtcgacaaaa ttactataag tatagaaaat
     1921 ttggaatttt accagaaacg gaatatgaaa ttaataaagt taaagaaatc gagcaatatg
     1981 ttaaagagca agaagaagct gaacaataga ggcgataaca tgaatttcga agaaaaatta
     2041 agtgaaatgt acaataaaat tgcaaatgag atcaatgaaa tgataccagt agagtgggaa
     2101 aaggtatatg taattgcata tgtggacgat ggaggtgggc aagttatatt ttattatacc
     2161 aagcccagaa atgatgagct gtattattat tcgagtatcg ttgaagatta taatgtttta
     2221 gaagaaatct ttgatgattt atggatggaa ctttatagat catttaaaaa attaagaaat
     2281 atatttaaag aagaaagtct tgaaccatgg acatcatgtg aatttgactt tacaaaagaa
     2341 gataaattaa aagtttcatt tgattatatt gattggaaga atacagaatt tgatcaatta
     2401 ggccaagaaa actactataa ctatagaaaa ttcggaatta taccagaaat ggaatatgaa
     2461 atggaagagg ttaaacaaat cgagcaatat attaaagagc aagaagaagc tgaacaatag
     2521 aggcgataac atgattttcg aagaaaaact aaatgaaatg tacaacgaga ttgcgaataa
     2581 aattagtagc atgataccag tagaatggga aaaggtatat acaatggctt atatagatga
     2641 tggaggaggt gaagtattct ttaattatac taaacagcga tgaattgaat tattacaccg
     2701 atatacctaa ggagtataac atttctgtgc aagtatttga tgatttatgg atggatttat
     2761 atgatttgtt tgaggaatta agaaatttat ttaaagaaga aggactagaa ccatggacat
     2821 catgcgaatt tgattttaca agagaaggta aattaaaagt ttcatttgat tatattgatt
     2881 ggataaattc agaatttggt caagtaggtc gacaaaatta ctataagtat agaaaatttg
     2941 gaattttacc agaaacggaa tatgaaatta ataaagttaa agaaatcgag caatatatta
     3001 aagagcaaga tgaagctgaa ctataggggc gataatatga ctttcgaaga aaaattaagt
     3061 gaaatgtata gcgagattgc gaataagatt attagcatga taccggtaga gtgggaaaaa
     3121 gtatatgcaa tggcatatat cgatgaagaa tgtggagaag tgttttacaa ttatactgaa
     3181 ccaagcagtg atgaattatt ttactataca agtgtgataa agaagtataa tttattgaaa
     3241 tcaagcttta tggattcagt atatgagttg catgatcaat ttgaggaact aagagaagta
     3301 tttatagaag aaggtcttga accatggaca tcatgcgaat ttgattttac aaaagaagat
     3361 aaattaaaag tttcatttga ttatattgat tggataaata cagagtttga tcaattaggc
     3421 cgtgaaaatt attatatgta caaaaaattt ggtgttttac cagaaacgga atatgaaatg
     3481 gaggaggtta aagaaatcga gcaatatatt aaagagcaag aagaatagac aatcttaatg
     3541 taagattaaa caataaagct ttgtttagtc tttttagcgt ttaagtaaaa aacaatagag
     3601 accataaagt tgtcatcaaa taataatata aagataattt taggctttta tacttttaat
     3661 cgaaaagaat ctaagtactt ataactttaa atacttaata tgctattaca aaatcaatgt
     3721 ataggagata ggtaaatatg actactaagg aaaatataga cattcttcga aagccaggtg
     3781 cacaagcctt aagtttagca tcattattta tgatactttt ttcatgtcta actttctttt
     3841 ttggtttaga ttatgaaagg tttccaaatt atttaaagat aacgacaatt atagaattaa
     3901 taattatcat aattagttta cttcaatgga ttagatttat agatttcgaa aaggaaagcg
     3961 cacagaaata taagaaaata tatgcccgat ttttagttat tataaatgtg ctaactacta
     4021 tcaccgcagt atttgcaaca tgtaaccttt attattttgt tgctgtacaa aatcattatg
     4081 acctattcaa ttattggttg atgggtacga tttcaatcat aattagttat ttgttattag
     4141 taattggcgg aatgttcacg ttattaaaat tacctaaagt aacaaaacgt tggggtggta
     4201 aaactaaaac acatttcggt ttattattaa ccgcgttgag cgcatttata tatattgaaa
     4261 gaattatcga atatatattg gtgcccaatg tcgtagaatc caagtttgtc ataatggtaa
     4321 gtatcattat tattgcttgt acacaatttg tggcatttca atttattatg caatacagta
     4381 gattctatat ttttgaatta aacactgaag atgatgacta aataatttta taaaatactg
     4441 ttggagtata cttaaataag aaaatcaact tcaacgtata tcaccatatt attttatact
     4501 caatgatgtg aaagaatttg agtggattga tattaatggg atttgattat tagaattaga
     4561 aaaggaacgt ttgcttttgt tgtaataaat ctattatttt tcaattgttg gttatttatt
     4621 ctg
//
LOCUS       DANJFZ010000160         4554 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.161, whole genome shotgun sequence.
ACCESSION   DANJFZ010000160 DANJFZ010000000
VERSION     DANJFZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.161"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            51..644
                     /locus_tag="PWN45_002576"
     CDS             51..644
                     /locus_tag="PWN45_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL0624311.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            628..1779
                     /locus_tag="PWN45_002577"
     CDS             628..1779
                     /locus_tag="PWN45_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDL0624312.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            1779..2387
                     /locus_tag="PWN45_002578"
     CDS             1779..2387
                     /locus_tag="PWN45_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL0624313.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            2453..2659
                     /locus_tag="PWN45_002579"
     CDS             2453..2659
                     /locus_tag="PWN45_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624314.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            2747..3457
                     /locus_tag="PWN45_002580"
     CDS             2747..3457
                     /locus_tag="PWN45_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDL0624315.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            3441..4445
                     /locus_tag="PWN45_002581"
     CDS             3441..4445
                     /locus_tag="PWN45_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL0624316.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         1663 a          640 c          818 g         1433 t
ORIGIN      
        1 acatccaata catattataa aaataaaaca catttagaac ggagtataca atgattctag
       61 tcatagataa taatgattca tttacatata atttaataga ctatattaag actcaaacga
      121 aactaacagt tcaagttgtt ggtattgata atctgctgat agaagacgtc attaatatga
      181 agccaaaagc aattgttatt tcgcctgggc cgggtaatcc ggatgattat cctatcttga
      241 atgaagtgtt agaacaattt tatcagcgtg tacctatact aggtgtatgt ttaggatttc
      301 aatgtatcgt gtcttatttt ggtggaaata tcattcacgg ctatcatcct gtacacggac
      361 atactacaca gttacgccat accaatgaag gtatttttca aggactgcct caaaatttca
      421 atgtaatgcg ttatcattca ttaattgctg acggagcgac ttttccaaat tgcttaaaga
      481 ttacagcaaa aaacgatgaa gcgattatta tggcatttga gcatattaga tttccggttt
      541 ttggtgtgca atatcatcct gaatctattt tgagtgaata cggttatcga caagttgaat
      601 tatttttatc gaaggtaggt gattactgtg agaatagaat ataattatcg ctactattta
      661 actgaaaatg aatataagca ataccatatt caattaaagg gatttataaa gaagtatgtt
      721 gctactaagt tggctgatgt gggagaagtg atacactttg cacaagcgca gcaacgacaa
      781 ggtagatatg tctcgttata tttaagttac gaagcggcaa agtattttaa tcatgctatg
      841 tgtacacatt cattagctaa agatgatatt tatgcagcag cttatagttt tgaaaaagcg
      901 gaaagcataa attcaacata tgaacatcaa acttcttatg tatcaaagta tcatttttca
      961 tttgttgaat cttctgaggt tatgatgact aatattaaac gtgtccaaca agcaattgtt
     1021 gaaggcgaaa cgtatcaagt gaactatacg gcgcgcttaa cggataacat ttattatcct
     1081 attagtactt tatatgaacg attaactcaa tttagtaatg gtaattatac tgcgttatta
     1141 caaactgatg aaatccaagt agcgtctatc tcaccagaat tattttttca aaaaggacaa
     1201 tttaacaatg tcgataacgt tatcataagc aaaccgatga aagggacaat gcctagaggt
     1261 aaaacggaag ctgaagatca acagtattat aaaacattgc aaacttcttc gaaagatcgt
     1321 gcagaaaatg tcatgattgt tgatttacta agaaacgata tagggagaat atcacagagt
     1381 ggctcaatta aggtgtataa actatttttt attgaggcat ataaaactgt atttcaaatg
     1441 acttcgatgg taagtggaac tttaaaaaat aatacagact taactcaaat tttaacatcg
     1501 ttatttcctt gtggttcgat tacaggtgca ccgaaactga atacaatgaa atatattaaa
     1561 caattagaaa gttcacctcg tggtatatac tgcggagcaa ttggactatt acttccaact
     1621 gaagatgata aaatgatttt taatattccg attcgcacta ttgagtataa atatggacaa
     1681 gcgatttatg gagtcggagc aggtattaca attgattcta agccaaaaga tgaagtgaat
     1741 gaattttacg caaaaaccaa gattttggag atgttataat gcaattattt gaaacaatga
     1801 aaattgataa tggacatatc cctagactta cttatcatac taatcgcata aaatgttctt
     1861 ctgagcgatt aaactttaaa tttgatgaac atgcatggcg aaatgaatta aacgatgtaa
     1921 caacaaagta tcacagtggt caatatagac ttaaaatcgt attaaatgct gaaagcaaat
     1981 ttgaaacgat agtgtcacct ttacctgaga aaagtagttt tacagcaaaa tttcaagtgt
     2041 tgcccaaagt agttaatcca acttttataa aaaataaaac gacagaacga aagcatttag
     2101 cacacaatca tgaaacagat ttaatattgc taacttcaga ggacggcaag gtccttgaat
     2161 ttgatattgg caacattgtc attgaagagg atggaaaatg gtacacacca agttataaag
     2221 atgatttctt aaaaggatgc atgcgtgatt atttaataga tagtgacaaa cttgttgaaa
     2281 aagactttaa taaaaacgaa ttgatttata aatatcataa caatgagata cgtttatttt
     2341 tgataaatag tttacgagag gttgccgatg tccacctttg cctttaaaat tagtatgata
     2401 ataaagtaga aaatgatgtg aaaacaaatt taaaaattaa atggggccaa atatgattat
     2461 tgtttatatt gtgctgttgt taattcttgt atacgtaaat tatcgattag tgaatcgatt
     2521 gctatctgaa aatagaatat atgttgttcg tttgatagca acaattacta ctgttataag
     2581 ctttatcctt gtatacgcat taattcacga acttatgcct tttgttgtgc gggcaatgga
     2641 tttaatgtac caccagtaaa tttagtgagt aggtaaaatg aactattcaa tcgattctat
     2701 aattaaaaat gaaagtaaag taaccaaata ttgaagaggt gtacaaatga aaatatatag
     2761 tcaaggtgac caagccattg tagtcgcaat tgaaaaagaa gtatctaaaa gtttaaccga
     2821 agatttatta acacttcgct catatttaat tgaacaaaat tatccattta ttatagaaat
     2881 tgtgccatca gaatcagaca tgatgattgt ctatgacgca agggatatga ttaaacacca
     2941 taatatacaa tcaccttttt tatacatgaa agcactaata gaatcgattc atttaaacat
     3001 aaaacatgat tttaaccagc aagatttgat tgaaatacca attgtgtatg gttcgaaata
     3061 tggtccggat ttagaatcac ttttaaaaca ttacaaaatc aagctagaaa cttttattga
     3121 attacattct aaggcgcaat attttgtttc gatgatggga tattcacctg ggtttcctta
     3181 tttaactgga ttaaataaga aattgtatat taatcacacg agtaaacaga aaaaattcat
     3241 tccagctggt tctgtagtac ttgaagggaa aaaatgcggt attgtaacta cggatacaat
     3301 taatgattgg ttagttattg gttatacacc attatcactt tttaatccga aagaatcaga
     3361 tttcgcacgc ttaaagttag gcgataatat taaatttaga cctatcaatg aaaatgaatt
     3421 agaagtagga gcgtttaaag atgtcaatca taattgaaaa aagtggctta ttcagtagct
     3481 ttcaggactt tggcagaagg ggatatgaac atgatggtgt aattccatgt ggtgcacttg
     3541 atactttagc acatgaaatt gctaatcgat tagttgcaaa tgacaagaat gaagcaactt
     3601 tggaaatgac taataaaatg gcaacgattc gttttacaga acctacgctg attgcattag
     3661 cagggggtaa tgtcaaagct tacactgagc atatgactat atctccatat aaattgtatt
     3721 tgttagataa aggcgatgtt ttaaagttta gagaaacaag ttatacatcg cgagtgtatt
     3781 tagctgtggg aggcggattt gaattagatg catggttagg atctaactca accgacttta
     3841 atgtaaaaat tggtggtttt aaaggtagaa cattacaaga tggcgatgaa ataaagctta
     3901 agagagatta tacagctcgt catcataagt tatttgaaaa ccttgctcac acgaaacaaa
     3961 cagattgggg tattgatgga tacgccttgt catttaatta tatgtctgat gtatttcatg
     4021 tcgttaaaaa taaaggtacg gaagatttta aagaagatgc cattcaaaga tttgtgaaac
     4081 atgattataa agtaacgagc aaagcaaatc gcatggggat gatgcttgaa ggtgaaaaaa
     4141 tcaaagcttt ttatgaagat atgccaccgt atcagactgt caaaaaagga acgatacaaa
     4201 ttaagcgtga tggcacacct attatcctat taaatgatca ttatacgcta ggtagctacc
     4261 cgcaaatcgg tacaatcgca agttatcatt taacgaaatt agcacaaaaa ccgcaaggat
     4321 cacgtttgaa atttcaattt atagatattt taacggctga aaagaacctt gttaagtata
     4381 gtaactggtt aaaccaatta ttccatggaa tagaatatag aatgcaatta gaaatgatga
     4441 aataatattt ggtacgtagc tcatactcga gtccgatgca aataatttcc tcctaatgta
     4501 taatgaaata atactgtgtt ttatctgcga aatgtatcat tttctaattc gttt
//
LOCUS       DANJFZ010000161         4506 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.162, whole genome shotgun sequence.
ACCESSION   DANJFZ010000161 DANJFZ010000000
VERSION     DANJFZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.162"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..400)
                     /locus_tag="PWN45_002582"
     CDS             complement(<1..400)
                     /locus_tag="PWN45_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HDL0624317.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQK"
     gene            complement(524..658)
                     /locus_tag="PWN45_002583"
     CDS             complement(524..658)
                     /locus_tag="PWN45_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDL0624318.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(715..849)
                     /locus_tag="PWN45_002584"
     CDS             complement(715..849)
                     /locus_tag="PWN45_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDL0624319.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1213..1286
                     /locus_tag="PWN45_002585"
     tRNA            1213..1286
                     /locus_tag="PWN45_002585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1247..1249,aa:Arg,seq:tct)
     gene            complement(1761..1949)
                     /locus_tag="PWN45_002586"
     CDS             complement(1761..1949)
                     /locus_tag="PWN45_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0624320.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            2198..3145
                     /locus_tag="PWN45_002587"
     CDS             2198..3145
                     /locus_tag="PWN45_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL0624321.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            complement(3450..3677)
                     /locus_tag="PWN45_002588"
     CDS             complement(3450..3677)
                     /locus_tag="PWN45_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624322.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(3985..>4506)
                     /locus_tag="PWN45_002589"
     CDS             complement(3985..>4506)
                     /locus_tag="PWN45_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL0624323.1"
                     /translation="MFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVL
                     NEGMISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLA
                     DTVGIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIF
                     GGGVLITQVLIYS"
BASE COUNT         1501 a          800 c          581 g         1624 t
ORIGIN      
        1 tcttttgcgt ttcggttaca ccattagtaa caatatataa atcatgtctt ttcgataatt
       61 cgacaattgt ttctaatgtt tgatcaaagt atttaacttt agcttctgct aatccatttc
      121 taaataacac atctgcacga tgcccatcaa cttccatttg atgatgtttg aagtaattca
      181 caaatcgttc tgataatact tcagacttcg ttaatttatt ttgttgaaaa gcttcccaat
      241 gttggtgatt gattttttta aatgttaaaa aatcatcctt tgttgcttta tgattaaaaa
      301 cattcgccat atagtgaaac gcccattctt ctgcatcata aaaatcaaca attgtatcat
      361 caaagtctat caaaatattt ttatatccca atttccccat ctcctatatt gtctatgtat
      421 ctaaatctta acagaggctc aaatttctgc aaataaaata aactgagtgc ataacattaa
      481 agtatgctca cccagtttat tttaaagaat attagttatt atattagaat ccaaataatt
      541 tacctagtaa acccacaccg ttagcaacga tgtctacgat acttgtgcct aatttcacac
      601 tatcatgttg ttgtgcagct tgcacagtat ttgcgattgc ttctgctagt ccagtcattt
      661 aaatctctcc ctcacctttg aaataatact gattacttac ataacatatt gaaattagaa
      721 tccgaataat ttacctaata aacctacgcc attttcaacg atgctcacaa tgcttgtgcc
      781 taattttgcg ccatcattat taattgctgc agttacggta tctttaattg cgttaaataa
      841 accttccatt gaaaacactc cttaaaattt aaatttgaag ataacaaaaa cgtgcgtagc
      901 ttttaaatca ccgaaatgtt attcgcttat cgttttgttg ttgttatttt aaaataaatt
      961 tgatacaatt agtttgttta tccgcacaac atcttataat gtacttaact gtattttaaa
     1021 gagaaaagaa atacagttag gcattcaaaa ctgtatttaa cacaattaag ttgcctgaat
     1081 tcgtatttaa gtcttattga acctttttag ataaatagct ctataatagt gaaaaatata
     1141 aacatttttt atttacaagg tattgctaat ttaagttcat ttagatataa taattcttgt
     1201 gttgttaaac gtgtcctggt agctcagctg gatagagcaa tggccttcta agccatcggt
     1261 cgggggttcg aatccctccc aggacgttta taggtatttt tatacgcatt accaaacaaa
     1321 agagttccgt gattacgggg ctctttttgt tttgaatttc agtaatatag tatgatgcgt
     1381 caccaaaacg tcccccgcat aagccccgaa aatacagtaa ttaaaacaag catgcttatt
     1441 cgttatagaa ttttttgaca cacaattgac acgcgtctga cacttgttta tacattttta
     1501 attaagtaat tttgtgctca aatttcatct atactgcacc tgaactacac caacactaca
     1561 ccaagatttt taacactcac catttgcatg cgtagagatt tttattatta tattattcct
     1621 atagattttg atactattca aaattttagg gacttttcag gggcccgaaa tcctataatt
     1681 ataattatat acatctaaaa aaaataacca cgtccatcga gacgtggttt tttaattatt
     1741 taactcacta attattcttc ttaattttca ttgctaaata tactattggc aaatttataa
     1801 agtagttcag cgtttttcaa tgacatattg tctaatgatc tttcattttt tctcattctg
     1861 tgtattgtgc tttgtggaac tcctgtttgt ttcgatatat gtaaactgct caaatcactg
     1921 tctaatagtt tttgaatttg atttctcatt ttgtacaccc ccttgtaaat cttcaatcat
     1981 catttaaatt aaataattac ttttccacat attccaatat ttaggttgca aagcatacct
     2041 caaatatcat taattttgag atttaaatgt caattatgtg tctatcaatc caatatacat
     2101 actctaataa cgtaatagta cacactcttc ttattaattg gccatagcta tcatgatata
     2161 attagtgaag aaaatcacat aagaaaggtt gtaaatcatg agacttcaaa aagcacctct
     2221 agtaacgtca ggactagtct taggattatt aggcctgggt aatctattaa aagacttatc
     2281 tcttacttta aacgctgttt gcggaatctt tgctttcttg atttggattc accttttatg
     2341 tactatgatc aaatatttta ataatgtgaa agaacaatta aacagtcctc tagtttcatc
     2401 agtgttcaca acatttttca tgtctggctt tttaggtact acttatttaa atacattttt
     2461 tagtaacata acttttatca atagcttaat aacgcctatt tggattttat gccttgtggg
     2521 aattatgacg catatgatta ttttttcaat aaaatattta aaagattttt cacttaaaaa
     2581 tgtttatcct tcgtggactg tactttttat tggtattgct atcgcaggat tgacggcacc
     2641 cgttagcgga tattttttca taggtcaatt aacagtaata tatggctttg tagctacttg
     2701 tattgtctta cctatagttt tcaagcgatt aaaagcattt ccattgcaga cgtcaatcaa
     2761 accgaacaca tcgacaattt gtgcaccatt ttctttagtc gctgcagcat atgttatagc
     2821 ttttcctaag gcgaatgctt ttatcgtaat tatattttta ctattagctc aaatatttta
     2881 tttttatatc attatacaat tgcctaaatt actaaaagaa cctttttcgc ccgtattttc
     2941 agctttcaca ttccctttag taatctcagc aactgcttta aagaacagtt tgcctgtact
     3001 tatgtttcca gacatttgga aaggtctttt gtttatcgaa gtgttattag ccactgtaat
     3061 agtacttaga gtctttatag gatatcttca cttcttttta aaaaaggaaa aacaagataa
     3121 atttcttcgt aatgcgtctc agtaacacta ttaccaagaa ttaacacgta tatttaataa
     3181 ctaaaaagag gcaggtaatc ttgcctcttt ttagtgtata tcaatgcctc tttgatttaa
     3241 tatgatctta tcaatgatac cttctgacta aagctatagt aactatacta gaacgtctag
     3301 ttcataaaca cgtttcactc taaactttat ttaaataata tataagcagc aaacgccaag
     3361 gctaatatac caataaaata attaataaag atttttattt tagtctttgt aatagttgac
     3421 aaattttatc acctctaaat atcgataaat taaacaccgt tatttttcct ttgcctatct
     3481 tttatcattt cagaaggtac tccgaaaata aacatcagaa taaataatat aattggcaaa
     3541 tgtggtatgt tttctagcaa ataccatctt aaattccaat caaacaccat atttgcatac
     3601 gccacaaaat atttactaaa taataatact acgcatattc cagttagtgt atctaaaacc
     3661 tttgtcaaat gtgtcattta tagccatcct ttatatatgc tattttcaca accatatgta
     3721 tcatgtgtta caaaaactgt ttacagcaaa tacatattta ccacgataaa tataatatac
     3781 catagatcta acattacatt aaaaacaaat tttaaaaata tcattattaa atagagcatt
     3841 gtatcaatta cttactagtg ttatcattac cgtcattatt ttcttaatca aaaataataa
     3901 tgcacctcgc ttagtttagt atttctacaa acctacgaga tgcataaaac cccattagta
     3961 ttttttataa tatagcattt caaattatga ataaattagt acttgcgtaa ttagtactcc
     4021 gccaccgaaa atcaatacaa aagcaactac cggccataca aatcggaacc aatgtgaata
     4081 tcgcatgttt aacatttgta gtgtggccat aacaagtcca gtcggcgcta agaataacat
     4141 cgcatattga ccaaattgat atgtcgtaac gatgacgaat cttggtatac ctactgtatc
     4201 agctagtggt gcaaagatag gcattgataa taccgctaag ccagaagatg atggcacgat
     4261 aaaacctaaa cagaagaaaa taaatagtaa tacgatgata aataatggtc cactcatatg
     4321 ttgaactaaa gatgatgaaa agtgtaagat tgtatctgaa atcatacctt cattcaacac
     4381 taaattaata cctcgagcta aaccaataat taaagataca cctactaaac ttgatgcacc
     4441 attgacaaat gcatctacag ttcctttttc tcccaatcca gatttacctg tcccagcaat
     4501 aaacat
//
LOCUS       DANJFZ010000162         4473 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.163, whole genome shotgun sequence.
ACCESSION   DANJFZ010000162 DANJFZ010000000
VERSION     DANJFZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.163"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            739..2802
                     /locus_tag="PWN45_002590"
     CDS             739..2802
                     /locus_tag="PWN45_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL0624324.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2820..3365
                     /locus_tag="PWN45_002591"
     CDS             2820..3365
                     /locus_tag="PWN45_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HDL0624325.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3793..3927)
                     /locus_tag="PWN45_002592"
     CDS             complement(3793..3927)
                     /locus_tag="PWN45_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624326.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            3891..3983
                     /locus_tag="PWN45_002593"
     CDS             3891..3983
                     /locus_tag="PWN45_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624327.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
     gene            4130..>4473
                     /locus_tag="PWN45_002594"
     CDS             4130..>4473
                     /locus_tag="PWN45_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDL0624328.1"
                     /translation="MKINIYKSIYNFQETNTNFLENLESLNDDNYELLNDKELVSDSN
                     ELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTTLQ
                     NNVYIIPFGQAY"
BASE COUNT         1688 a          575 c          771 g         1439 t
ORIGIN      
        1 acttatctct aaatcatttt gagctactta tctatcaatt catatattct ataacaatat
       61 ttgtgacatc acgtgctatt tcatgaagtg attttacgat atcaccttct ttagaaaaaa
      121 tatatttcag taggcaccga cgtatacaga atcatttgag tattaaaaat aaaaactaga
      181 aaaagaaacc gcatcattaa ctgatacgca gaatcatatt ataaataaaa ctaaaaatga
      241 ggttgtatat aactcactct gaaattgatt gaatatatag tatctttaat aaaatgcagc
      301 tattgtggcg tagaatttga gaatcaaaaa atgattaata tagtttgaag agactgagca
      361 taaatactag aaaaatggcc agtaaatgag tttactataa actcatttac tggcttctct
      421 aataattatc aagacaattt gcgtttctag gcatactttg aaatgcgcta ttttctttaa
      481 gaatattaat atatgacttg tgttggtaat gataaccttg gttagagtgt attgtcatgt
      541 gatagttaag attagatctt ttaattaaaa cattgtttta aatatcgatg acaaggtcta
      601 atgtaggacg tgtagatata aggttcggaa ttatataaat ccataaaagg agataaatcg
      661 aaaaccttta taccctgggt gggtatatgt tatagtataa gtagctttac tataacattt
      721 tcattaggag gggttaattt gaataataat ggtgaagagc ataatcatca aaatcacatg
      781 aatcattcca atcaaatgca tcatgataac catgcctcac atgatcatca tagtggccat
      841 gcacatcatc atggaaattt taaagttaag ttttttgttt cattaatttt tgcaatacct
      901 atcattcttt tatcgccact gatgggtgtt aacttacctt ttcaattcac atttccaggt
      961 tctgaatggg tagtgttaat attaagtaca attttattct tttatggtgg taaaccgttc
     1021 ttgtctggtg gtaaagatga aattgctaca aaaaaaccag gcatgatgac cttagttgcc
     1081 ctaggtattt cagtagctta tatttatagc ttgtatgctt tttatatgaa taactttagt
     1141 agtgcaactg gtcatacaat ggactttttt tgggaattag caaccttaat tttaattatg
     1201 ctattaggac attggataga aatgaatgct gtcggaaatg ctggagatgc tttaaagaaa
     1261 atggcagaac tgttacctaa tagtgctatt aaagttatgg ataatggcca acgcgaagaa
     1321 gttaaaatat cagacatcat gactgatgat atcgtcgaag taaaagccgg agaaagcatt
     1381 ccaacagatg gtattatcgt tcaaggacaa acatctatag atgaatccct agtcactgga
     1441 gaatctaaaa aagtacaaaa aaatcaaaat gacaacgtca tcgggggttc tattaatggg
     1501 tctggaacaa tacaagtcaa ggttacagct gtgggagaag atggatatct ttctcaagtt
     1561 atgggacttg ttaatcaagc acaaaatgat aaatctagtg ctgaattgtt atctgataaa
     1621 gtagcgggtt atttattcta ctttgctgta agtgttggcg tgatttcttt tattgtctgg
     1681 atgctcattc aaaatgatgt tgattttgca ttagaacgtc ttgtaactgt gttagtcatt
     1741 gcttgtccac atgctttagg cttggcaata cctttagtca ctgcacgttc tacttcaatt
     1801 ggtgcacata atggtttaat tattaaaaat agagagtctg tagaaatagc tcaacatatc
     1861 gattatgtaa tgatggacaa aactggtact ttaactgagg gtaacttttc tgtgaatcat
     1921 tatgagagct ttaaaaatga tttgagtaat gatacaatat taagcctttt cgcctcatta
     1981 gaaagtcaat ctaatcaccc attagctata agtattgttg attttgcgaa aagtaaaaat
     2041 gtttcattta ctaacccaca agacgttaat aatattccag gtgtcggatt agaaggtcta
     2101 attgataata aaacatataa aataacaaat gtctcttatc ttgataaaca taaacttaat
     2161 tatgacgatg acttgtttac taaattagct caacaaggta attcaatcag ttatttaatt
     2221 gaggatcaac aagtcattgg catgattgct caaggagatc aaattaaaga aagctcaaaa
     2281 caaatggtag ctgatttact atcaagaaat attacaccag tcatgcttac aggtgacaat
     2341 aatgaagtgg cacacgctgt cgcaaaagaa ttaggtatta gtgatgtcca cgcacaactc
     2401 atgccagaag ataaggaaag cattataaaa gattatcaaa gtgacggtaa taaagtcatg
     2461 atggtcggag acggtatcaa cgatgcgccg agtcttataa gagcggatat tggtatagca
     2521 attggtgcag gtacagatgt tgcagtggat tcaggtgata tcatacttgt taaaagtaat
     2581 ccatcagata tcattcattt cttgaccctt tcaaataata ctatgagaaa aatggtgcaa
     2641 aacttatggt ggggtgcagg ttataatatt gttgctgtac ctttagcagc tggtatttta
     2701 gcatttattg gcttgatttt atcacctgca ataggtgcta ttttaatgtc tttaagtaca
     2761 attatcgttg caattaatgc ctttacatta aaattaaaat aaaagatagg agttttatta
     2821 tgattaaaaa attatttttt atgatattag gatcattact aatattatca gcttgctcca
     2881 ataatgatga aaaagataaa gacactaatg accaaaaaag tgagagccat atgaagcata
     2941 atgatgaaag taaagttcca gaagatatga aatcgactaa tgagggtgaa tttaaagtgg
     3001 gagataaagt aacgattaca gcagggcata tgccaggtat gaaaggtgca gaagctactg
     3061 taaaaggtgc gtataaaaca tatgcctatg ttgtaagtta taaacccaca aatggaaatg
     3121 aaaaagtaaa caatcataaa tgggtcgtaa acgaagagat taaagatgca cctaaagatg
     3181 gatttagtaa gggcgatact gttaaattag aagcaagtca tatgtctggt atgaaaggtg
     3241 ctacagccaa tatagataac gtgaaaaaga ctactgttta cgtagttgat tacaaatcca
     3301 aagataatgg taaaatcatt aaaaatcata aatggatgac aggaaatgag ctgaaagcac
     3361 gataaaaatc tagttctaaa ttgagaaata aatagatata aaaatatcct ccttaatcaa
     3421 taatttaaat aacttattat tgttaaggag gatatttttt agtgtgtaaa ttaaaaagaa
     3481 ttttagaaga ataacattta tcaaaaaact gttcattacc ttattaattg aaattatata
     3541 attaaaaacc gcatcattaa ccgatacgca gaggcgtatc ataagtaaaa ctaaaaaatt
     3601 ctgtatgagg agataataat ttggagggtg ttaaatggtg gacattaaat ccacgttcat
     3661 tcaatatata agatatatca cgataattgc gcatataact taagtagtag ctaacagttg
     3721 aaattaggcc ctatcaaatt ggtttatatc taaaatgatt aatatagaat gcttcttttt
     3781 gtccttatta aattataaaa gtaactttgc aatagaaaca gttatttcat aatcaacagt
     3841 cattgacgta gctaagtaat gataaataat cataaataaa attacagata ttgacaaaaa
     3901 atagtaaata taccaatgaa gtttcaaaag aacaattcca agaaattgag aatgtaaata
     3961 ataaggtcaa agaattttat taagatttga aagagtatca atcaagaaag atgtagtttt
     4021 ttaataaact atttggaaaa taattatcat aatttaaaaa ctgacaattt gcgagactca
     4081 taaaatgtaa taatggaaat agatgtaaaa tataattaag gggtgtaata tgaagattaa
     4141 tatttataaa tctatttata attttcagga aacaaataca aattttttag agaatctaga
     4201 atctttaaat gatgacaatt atgaactgct taatgataaa gaacttgtta gtgattcaaa
     4261 tgaattaaaa ttaattagta aagtttatat acgtaaaaaa gacaaaaaac tattagattg
     4321 gcaattatta ataaagaatg tatacctaga tactgaagaa gatgacaatt tattttcaga
     4381 atccggtcat cattttgatg caatattatt tctcaaagaa gatacaacat tacaaaataa
     4441 tgtatatatt ataccttttg gacaagcata tca
//
LOCUS       DANJFZ010000163         4457 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.164, whole genome shotgun sequence.
ACCESSION   DANJFZ010000163 DANJFZ010000000
VERSION     DANJFZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.164"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            125..217
                     /locus_tag="PWN45_002595"
     CDS             125..217
                     /locus_tag="PWN45_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624329.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            395..1228
                     /locus_tag="PWN45_002596"
     CDS             395..1228
                     /locus_tag="PWN45_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0624330.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1510..2307
                     /locus_tag="PWN45_002597"
     CDS             1510..2307
                     /locus_tag="PWN45_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624331.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2343..3347
                     /locus_tag="PWN45_002598"
     CDS             2343..3347
                     /locus_tag="PWN45_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0624332.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3344..4360
                     /locus_tag="PWN45_002599"
     CDS             3344..4360
                     /locus_tag="PWN45_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0624333.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
BASE COUNT         1462 a          638 c          859 g         1498 t
ORIGIN      
        1 accgaaattg aagcattata aaaaccaagt gcacatggta atacacttgg cttttatggg
       61 aaatgaatat tattgtacat atgacagtaa ggactaggta cagtcatagt acttcgagca
      121 aaatttgttt tgttattata aacaacacaa aggagataac ttctctattg aagaagttaa
      181 aaacattata gcagacaatg aaatgaaagt aaattaaaaa ttcagaatat ttttaattat
      241 tatattgtga gtgatattta ttagggaaag ctattcttca tataaattag ttaaatagta
      301 aatctttgtt agaactcttt ccatagagtt gtgacagcat ttttgttgtg ctactatttt
      361 tatagtctaa taaatatata aaggggatgg tttcgtgaat aaaacggtta aagatttaat
      421 actagttgtc ttaggttcat ttatctttgc tgcaggtgta aatgcattta ttatttctgg
      481 taacttaggt gaaggcgggg ttacaggttt agcaattatt ttatattatg cgtttcatat
      541 ttcaccagcc atcactaact tcttggtcaa cgcagtattg attgccatag gttataaatt
      601 tttgagtaag agaagtatgt acttaactat tcttgtaaca attcttattt caatattttt
      661 gagtttaaca gaatcatggc aagtagaaac tggaaacagc attgtgaatg ccatttttgg
      721 tggtgtaagc gttggactag gaatcggagt aattatcctt gcaggcggta caacagcagg
      781 tacaacaatt ttggcgagaa ttgcaacgaa atacctcgat gtaagcacgc catatgcttt
      841 gcttttcttc gatatgatcg ttgttgcaat ttcacttaca gttattccac ttgataaagt
      901 attagtaaca gtaatatcac tttatatagg aacaaaagtg atggaatatg tcatagaagg
      961 tttaaacact aaaaaagcta tgacgattat ttcaactaat cccgacaaac ttgccaaagc
     1021 aatagacgag caaattggaa gaggtttaac cattttaaac ggacatggct attatacgcg
     1081 tgaagaaaaa gatgtcttat acgttgttat ttctaaaaca caagtttcaa aagcaaagcg
     1141 attaattaaa caaatcgata aagatgcatt cctcgtaatt catgatgtaa gagatgtcta
     1201 tggtaatggc tttcttgcag atgaataaat aaatggtatg agcacacata cttaaataga
     1261 agtccacgga caagtttttg aactatgaag acttatctgt gggcgttttt tattttataa
     1321 aagtaatata caagacatga caaatcgagc tatccaattt aaaaagtaat gttagtcaat
     1381 aagattgaaa aatgttataa tgatgttcat gataatcatt atcaattggg atgtctttga
     1441 aaattgataa tttaaaaata gaaattattt tttataaaca gaaagaattt tattgaaagt
     1501 agggaaatta tgaatcgttt gcatggacaa caagttaaaa ttggttacgg ggataacacg
     1561 attataaata aattagatgt tgaaatacca gatggcaaag tgacgtcaat cattggtcct
     1621 aacggctgcg ggaaatctac tttgctaaag gcattgtcac gtttattggc agttaaagaa
     1681 ggcgaagtat ttttagatgg tgaaaatatt catacacaat ctacgaaaga gattgcaaaa
     1741 aaaatagcca ttttacctca atcacctgaa gtagcagatg gcttaactgt tggggaatta
     1801 gtttcatatg gtcgttttcc acatcaaaaa ggatttggta gattaactgc tgaggataag
     1861 aaagaaattg attgggcaat ggaagttaca ggaactgata cattccgaca ccgttcaatc
     1921 aatgatttaa gtggtggtca aagacaacgt gtttggattg caatggcatt agcacaaaga
     1981 actgatatta tctttttaga cgaaccaaca acatatttag atatctgtca tcaattagaa
     2041 atactagaat tagttcagaa gctaaatcag gaacaaggtt gtacaattgt catggttctt
     2101 catgatatca accaagcgat tcgtttctca gatcatctta ttgcgatgaa agaaggggat
     2161 atcatcgcta caggttcaac agaagacgta ttaacacagg aaatattaga aaaagttttt
     2221 aatattgatg ttgttttaag taaagatcct aaaactggaa aacctttact ggtaacttat
     2281 gacttatgtc gcagagctta ttcttaatta agtaagttaa tatgataaaa aggacaatta
     2341 acatgacaaa tagagagaac ccaacgccat tgaagttttt atcctatatt ataggtttaa
     2401 gtatgatact actaatcaca ctatttattt ctacattaat aggtgacgcc aaaattcaag
     2461 cctctacaat tatagaggct atttttaatt ataatcctag caatcaacag caaaacatca
     2521 tcaatgagat taggattccc agaaatatag cagcagtaat tgtaggtatg gcgcttgcag
     2581 tttctggtgc gattatacaa ggtgttactc gtaatggtct tgctgatccg gcgctcatag
     2641 gtttaaattc aggtgcttca tttgctttag cattaacata tgcagtttta ccaaacactt
     2701 catttttaat attgatgttt gctggatttt taggtgctat tctaggaggt gctattgtat
     2761 taatgatagg ccgatctaga cgtgatggat ttaatccgat gcgtattatt ttagcgggtg
     2821 cagcagtaag tgctatgtta acagcgctaa gtcaaggtat tgcattagct tttagactaa
     2881 atcaaacagt aacattttgg actgctggag gcgtttcagg cacaacatgg tcacacctta
     2941 agtgggcaat tccattaatt ggtattgcgt tattcattat attaacaatt agtaaacaac
     3001 ttaccatttt aaatcttggt gaatcattag ctaaaggttt aggtcaaaat gtaacaatga
     3061 tcagaggcat atgtttaatt attgctatga ttctagcagg tattgcagtt gctatcgctg
     3121 gacaagttgc atttgtaggt ttgatggtac ctcatatagc aagattttta attggaactg
     3181 attatgctaa aattctacca ttaacagcct tgttaggtgg gatactcgtg cttgttgccg
     3241 atgtgatagc acgatattta ggagaagcgc ctgttggtgc aatcatttca tttatcggtg
     3301 ttccttactt tttatattta gttaaaaaag gaggacgctc aatatgatta gttcaaataa
     3361 taaacgcaga caattgatag cactggctgt ttttagcatt ctactatttc taggttgtac
     3421 ttggagtatt acctcaggtg aatacaacat acctgttgaa agatttttca aaactttaat
     3481 tggacaaggt gatgccattg ataagttaat cttattagat ttcaggttac ctcggatgat
     3541 gattactatt ttggctggcg cagcgcttag tattagtggt gcaatagtgc aaagtgtcac
     3601 aaaaaatcca atagctgaac caggtatatt aggtattaac gcaggtggcg gatttgcaat
     3661 cgcattattt attgcaattg gtaaaattaa tgctgacaac tttgtttatg tactgccgtt
     3721 aataagtata ctaggtggta tcaccactgc attgattatt tttattttca gttttaataa
     3781 aaatgaaggt gttacacctg cgagtatggt attaataggt gtaggtttac aaacagcatt
     3841 atatggtggc tcaattacaa ttatgtcaaa atttgatgat aagcaatctg atttcatcgc
     3901 tgcttggttt gcaggtaata tttggggtga cgaatggcca tttgtcattg catttttacc
     3961 gtgggtgttg attattattc cttacttact atttaaatcg aatacactaa atattattca
     4021 tacgggtgat aatattgcac gaggtctagg tgtaaggtta agcagagaac gtttaatatt
     4081 attctttatc gcagtgatgt tatcatctgc tgctgtagca gtagcaggtt caatttcgtt
     4141 tatcggatta atgggtccgc atattgccaa acgtatcgtt ggaccacgtc accagttgtt
     4201 tttaccaatt gccattttag taggggcatg tttacttgtt atagctgata caattggcaa
     4261 aattgtatta caaccaggtg gggttccagc aggtattgtc gtagcaatta ttggtgcacc
     4321 gtatttctta tatttaatgt acaaaacgaa aaatgtatag tgtcaatgga cacaacttat
     4381 tgctatgaaa ggcactttat tataaggctt ttcatagcat tttttattta atgagccact
     4441 caagactatt tattttt
//
LOCUS       DANJFZ010000164         4415 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.165, whole genome shotgun sequence.
ACCESSION   DANJFZ010000164 DANJFZ010000000
VERSION     DANJFZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.165"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            45..4190
                     /gene="sdrD"
                     /locus_tag="PWN45_002600"
     CDS             45..4190
                     /gene="sdrD"
                     /locus_tag="PWN45_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HDL0624334.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
BASE COUNT         1806 a          682 c          789 g         1138 t
ORIGIN      
        1 aacaattgta ttatttaaaa atttgatgaa ttaggagtaa tctaatgcta aacagagaaa
       61 ataaaacggc aataacaagg aaaggcatgg tatccaatcg attaaataaa ttttcgatta
      121 gaaagtacac agtgggaaca gcatcaattt tagtaggtac aacattaatt tttggtctgg
      181 ggaaccaaga agcaaaggct gcagaaagta ctaataaaga attgaacgaa gcgacaactt
      241 cagcaagtga taatcaatcg agtgataaag ttgatatgca gcaactaaat caagaagaca
      301 atactaaaaa tgataatcaa aaagaaatgg tatcatctca aggtaatgaa acgacttcaa
      361 atgggaataa attaatagaa aaagaaagtg tacaatctac cactggaaat aaagttgaag
      421 tttcaactgc caaatcagat gagcaagctt caccaaaatc tacgaatgaa gatttaaaca
      481 ctaaacaaac tataagtaat caagaagcgt tacaacctga tttgcaagag aataaatcag
      541 tggtaaatgt tcaaccaact aatgaggaaa acaaaaaggt agatgccaaa actgaatcaa
      601 ctacattaaa tgttaaaagt gatgctatca agagtaatga tgaaactctt gttgataaca
      661 atagtaattc aaataatgaa aataatgcag atatcatttt gccaaaaagt acagcaccta
      721 aacgtttgaa tacaagaatg cgtatagcag cagtacagcc atcatcaaca gaggctaaaa
      781 atgttaatga tttaatcaca tcaaatacaa cattaactgt cgttgatgca gataaaaaca
      841 ataaaatcgt accagcccaa gattatttat cattaaaatc acaaattaca gttgatgaca
      901 aagttaaatc aggtgattat ttcacaatta aatactcaga tacagtacaa gtatatggat
      961 tgaatccgga agatattaaa aatattggtg atattaaaga tccaaataat ggtgaaacaa
     1021 ttgcgactgc aaaacatgat actgcaaata atttaattac atatacattt acagattatg
     1081 ttgatcgatt taattctgta caaatgggaa ttaattattc aatttatatg gatgctgata
     1141 caattcctgt tagtaaaaac gatgttgagt ttaatgttac gataggtaat actacaacaa
     1201 aaacaactgc taacattcaa tatccagatt atgttgtaaa tgagaaaaat tcaattggat
     1261 cagcgttcac tgaaacagtt tcacatgttg gaaataaaga aaatccaggg tactataaac
     1321 aaacgattta tgtaaatcca tcggaaaatt ctttaacaaa tgccaaacta aaagttcaag
     1381 cttaccactc aagttatcct aataatatcg ggcaaataaa taaagatgta acagatataa
     1441 aaatatatca agttcctaaa ggttatacat taaataaagg atacgatgtg aatactaaag
     1501 agcttacaga tgtaacaaat caatacttgc agaaaattac atatggcgac aacaatagcg
     1561 ctgttattga ttttggaaat gcagattctg cttatgttgt aatggttaat acaaaattcc
     1621 aatatacaaa tagcgaaagc ccaacacttg ttcaaatggc tactttatct tcaacaggta
     1681 ataaatccgt ttctactggc aatgctttag gatttactaa taaccaaagt ggcggagctg
     1741 gtcaagaagt atataaaatt ggtaactacg tatgggaaga tactaataaa aacggtgttc
     1801 aagaattagg agaaaaaggc gttggcaatg taactgtaac tgtatttgat aataatacaa
     1861 atacaaaagt aggagaagca gttactaaag aagatgggtc atacttgatt ccaaacttac
     1921 ctaatggaga ttaccgtgta gaattttcaa acttaccaaa aggttatgaa gtaacccctt
     1981 caaaacaagg taataacgaa gaattagatt caaacggctt atcttcagtt attacagtta
     2041 atggcaaaga taacttatct gcagacttag gtatttacaa acctaaatac aacttaggtg
     2101 actatgtctg ggaagataca aataaaaatg gtatccaaga ccaagatgaa aaaggtatat
     2161 ctggcgtaac ggtaacatta aaagatgaaa acggtaacgt gttaaaaaca gttacaacag
     2221 acgctgatgg caaatataaa tttactgatt tagataatgg taattataaa gttgaattta
     2281 ctacaccaga aggctataca ccgactacag taacatctgg tagcgacatt gaaaaagact
     2341 ctaatggttt aacaacaaca ggtgttatta atggtgctga taacatgaca ttagatagtg
     2401 gattctacaa aacaccaaaa tataatttag gtaattatgt atgggaagat acaaataaag
     2461 atggtaagca ggattcaact gaaaaaggta tttcaggcgt aacagttaca ttgaaaaatg
     2521 aaaacggtga agttttacaa acaactaaaa cagataaaga tggtaaatat caatttactg
     2581 gattagaaaa tggaacttat aaagttgaat tcgaaacacc atcaggttac acaccaacac
     2641 aagtaggttc aggaactgat gaaggtatag attcaaatgg tacatcaaca acaggtgtca
     2701 ttaaagataa agataacgat actattgact ctggtttcta caaaccgact tacaacttag
     2761 gtgactatgt atgggaagat acaaataaaa acggtgttca agataaagat gaaaagggca
     2821 tttcaggtgt aacagttacg ttaaaagatg aaaacgacaa agttttaaaa acagttacaa
     2881 cagatgaaaa tggtaaatat caattcactg atttaaacaa tggaacttat aaagttgaat
     2941 tcgagacacc atcaggttat acaccaactt cagtaacttc tggaaatgat actgaaaaag
     3001 attctaatgg tttaacaaca acaggtgtca ttaaagatgc agataacatg acattagaca
     3061 gtggtttcta taaaacacca aaatatagtt taggtgatta tgtttggtac gacagtaata
     3121 aagacggcaa acaagattca actgaaaaag gtatcaaaga tgttaaagtt actttattaa
     3181 atgaaaaagg cgaagtaatt ggaacaacta aaacagatga aaatggtaaa tactgctttg
     3241 ataatttaga tagcggtaaa tacaaagtta tttttgaaaa gcctgctggc ttaacacaaa
     3301 caggtacaaa tacaactgaa gatgataaag atgcagatgg tggcgaagtt gacgtaacaa
     3361 ttacggatca tgatgatttc acacttgata atggatacta cgaagaagaa acatcagata
     3421 gcgactcaga ttcggacagc gactcagatt cagacagaga ctcagactca gatagtgatt
     3481 cagactcgga tagcgattca gattcagaca gcgattcaga ttcagatagc gattcagatt
     3541 cagacagaga ctcagatagt gattcagact cagatagcga ctcagattca gacagcgact
     3601 cagattcaga cagcgactca gactcagata gtgattcaga ctcagatagc gactcagatt
     3661 cagacagcga ctcagactca gacagcgact cagactcaga tagtgactca gattcagata
     3721 gcgactcaga ttcggacagc gattcagact cagatagcga ctcagattca gatagcgatt
     3781 cggactcaga tagcgactca gattcagata gtgattcaga ctcagatagc gactcagatt
     3841 cagacagcga ctcagattcg gatagcgact cagattcaga cagcgactca gactcggata
     3901 gtgattcaga ctcagatagc gactcagact cagatagcga ttcagattca gatagcgact
     3961 cagactcaga cagcgattca gactcagaca gcgactcaga ctcagatgca ggtaagcaca
     4021 cacctgttaa accaatgagt actactaaag accatcacaa taaagcaaaa gcattaccag
     4081 aaacaggtaa tgaaaatagc ggctcaaata acgcaacgtt atttggcgga ttattcgcag
     4141 cattaggatc attattgtta ttcggtcgtc gtaaaaaaca aaataaataa tacagcttat
     4201 accaggtccg tgaggcctgg ttttttattt caaaatttat gaaatgtatc ttatgtttga
     4261 gattaaatag tgcaattttt taaaaaatat aacataaatc aacataaatt taattcatct
     4321 ttacattaaa agtacaaacg aataagaaaa gcaattaaac acagtatcaa accatttaaa
     4381 acaattgata taaaaaacaa acgaataatt aaaaa
//
LOCUS       DANJFZ010000165         4272 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.166, whole genome shotgun sequence.
ACCESSION   DANJFZ010000165 DANJFZ010000000
VERSION     DANJFZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.166"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            98..469
                     /locus_tag="PWN45_002601"
     CDS             98..469
                     /locus_tag="PWN45_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL0624335.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(524..1768)
                     /gene="fabF"
                     /locus_tag="PWN45_002602"
     CDS             complement(524..1768)
                     /gene="fabF"
                     /locus_tag="PWN45_002602"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL0624336.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1780..2721)
                     /locus_tag="PWN45_002603"
     CDS             complement(1780..2721)
                     /locus_tag="PWN45_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL0624337.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3016..3201
                     /locus_tag="PWN45_002604"
     CDS             3016..3201
                     /locus_tag="PWN45_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL0624338.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3458..3892)
                     /locus_tag="PWN45_002605"
     CDS             complement(3458..3892)
                     /locus_tag="PWN45_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDL0624339.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(4203..>4272)
                     /locus_tag="PWN45_002606"
     CDS             complement(4203..>4272)
                     /locus_tag="PWN45_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDL0624340.1"
                     /translation="FLKEVNQHKIDQTSINLIGRKI"
BASE COUNT         1335 a          776 c          605 g         1556 t
ORIGIN      
        1 aatttcaata aagctgaaga aattaaaaaa tcattcctca atgtctgatt ttaatgtaaa
       61 ctatgcgtaa gagttttcta taagtgggag tgatcaaatg ttgaaaaaat ggcttggaat
      121 ggcactaata acgccaatat taacgtttat tatatggctt tttaatagcc atactatcat
      181 tacatattta aatatattgt tttatgtttc attaattatt tttattagca tatttttgat
      241 tttacttgta caagaaggca tttttgatgc gacaagttat ggttttagaa gattaaagta
      301 tcaaatgtca tcttcgaaaa agaagaagtc tatctctgat gatccatttt tcaacccaca
      361 agaggtaaaa aaagagcatt actttgtttc tacatggata atccccttat tattaataaa
      421 tattttatat tttattatga cgattgtgct gtcgctcatc ttagtataaa gaaactgaaa
      481 aaatcttcag taactaaaac aacgttactg aagattttta tagttatgct tcaaatttct
      541 tgaatactaa tactgcgtta tgtccaccga atcctaagct attactcatt gcataagtaa
      601 tatcaaggtc ttgcgcttca tttggaacaa tatccaaatc acattctgga tctggtgtta
      661 ccgcatgaat tgtcggtgcg actttagagt ctttaattga aagcgctgag aagattgctt
      721 caattccacc tgttgcacca agtaagtgac cagtcattga ttttgttgag ttaactttta
      781 agtgtttagc tgcttcacca aatgtatttt taatagcttt aacttcattt aagtcaccaa
      841 caggagtact tgtaccatgg gcatttaagt attgtacatc tttaggttca ataccagcat
      901 catccatagc tgcttgcatt gctctagaac caccttcacc ttctggagct ggcgctgtaa
      961 tatgataagc atcacctgta gtaccatagc caactatctc agcataaata ttggcacctc
     1021 gagcttgtgc tgattctaaa gattcaatta ctaaaatacc agcaccttca cccataacaa
     1081 aaccatctct accttcttgg aatggacgac atgctgtttc aatgtcatca tttgtagaaa
     1141 gcgctcgact tgcactgaaa ccagcaattg ccatatgagt aattggtgct tctgtaccac
     1201 cagtaatcat tgcatctgca tcaccgcgtt gcacaatttt aaatgcttct ccgattgaat
     1261 ttgtacctgt tgcacatgct gtaactgttg caccatttgg tccttttgca cctaagtcaa
     1321 ttgatacttg cccagttgcc atatcaggaa ttaacattgg tacgaaaaat ggactcacac
     1381 gtcttgggcc tttatccatt aattgtttat gtgcaatttc aaatgtttcc ataccaccga
     1441 taccagaacc aatccataca ccgattcgat ctgcagtatt ttcattgata tctaattgcg
     1501 catctttaac agcctctcta gctgctacaa ttgcatattg agtaaatcta tccatacgac
     1561 gcgcttcttt tttgtcgata tgatcttcaa tattaaagtt tttaagttct cctgctaagt
     1621 gaacgctata aggttcagta tcgatacgtg taattttatc gataccattt acgcctttta
     1681 gagcattctc ccatgttgtt ttgacatcat taccgattgg agaaagggct cccatacctg
     1741 taataactac tcttttattt tgactcattc gttatcctcc tattttcccc attttattgt
     1801 cattgcgccc caagttaggc cgccaccgaa tccgacaaga acaattgtat catcatcttt
     1861 gattttacca ttttttaatt cttgatcgat acttaaaggt attgacgcag ctgaagtatt
     1921 tccatattta tttacagaaa cactcatttt gtcttttgaa atacctaagc gttctctagc
     1981 tgattccata attctaatat tagcttgatg aggaataaat aaatctatat catctgatgt
     2041 taaattcgct ttttcaacta cacgtgttga tgcatcaccc ataattctaa cagcaaattt
     2101 aaatacttct cgaccattca ttttcagttt accagtatct ttatctaaat ataaatgttt
     2161 accacctgtg ccatcagaac ccatttcata acttataata cctctgccat ctgaaacttc
     2221 accgatgata accgcacctg caccatctcc aaatagaact gcagtagaac ggtcagttaa
     2281 atctgttatt ttagataatt tatctgcacc gacaactaaa atgttatgat aatctccaga
     2341 ttgaacatat tgtttagctg taatcattga atacataaat ccagaacatg ctgcaagttg
     2401 atccatagag gcaactttgc ccgtccctaa acgttcttgc aacatatttg cgacagttgg
     2461 aaatggcata tctccagttg ctgtggcaac aattatcata tctatatctt cgggctgaat
     2521 accagcgtca gcgattgctt ttaaacttgc ttcatatgct aaatctgaag tatcttgatc
     2581 atcatctgcc caatgtcttt ctttaattcc agtcatctta gaaatccatt catcagatgt
     2641 atctaaaaat tgctcaaaat aggcattgtc aataatcttt tctggcgcat atgcaccaaa
     2701 acctttaata cccacgttca tagttgaaca ccttcttaat aaaattttta ataccaggta
     2761 ttaatttaac atagattact ttttaaactc aagtcagagg taggaatatt gactagttaa
     2821 taaaatttta atgctttatt atcgtcaaat ttcagtatga aattggtttt aatgcgctta
     2881 atttagaata gccattcaga taaaatcatt tatttaaact gtataatatg gtcaaaatat
     2941 atgagatgtt actatacaaa tttctaactt tcacttaaaa taaaatatat ataattaaag
     3001 tggaggtaga atgacatgaa acacctcgta acattctttt gggcattatt attaatgcaa
     3061 atggttaact ttgtacttaa tagcctagta ggtggagaca gcttaaatgt agtgaatcca
     3121 attatcatgg cagtattgtt cacgattttc acagcaattt ttgctgcagt aattaaaccg
     3181 ccgaaagatt catcacaata actatttagt ataaaatgaa atatgtcttt cttattattg
     3241 aagctgccca gataaaggcg gcttttattt ttggccatta atgttttgta ttttgaatat
     3301 tttaaagctc aatgacctct aacccatcac ttttaaattt agaaaaagcc ttaatacaaa
     3361 gatgtcattt acgagtaatc tttgtatcaa ggcaattatt ttataatcat cactgtaaaa
     3421 ttaaatgctt catgttgaat ttgataattt taagtgttta tttagttttt acattaatat
     3481 caatttgttt aattgaattt gaatcaaaca agtttgctgt gtagctatct ttcttaagat
     3541 ccacttcttt tttactacca tctttccaag taatcgtgta aacggcagat tttgcatttc
     3601 gaatcttctc aggtgttaca ccgcgttcat catataacaa agctttaact ttatttccta
     3661 aatctaaata tgataattgg ctatcttttg gtaaatttaa gtaagattga ttgaaagcca
     3721 taatgccatc tactgcaatt gtatatggaa cattttgcat ttctttagct ttttgagttg
     3781 ccggtagttt gtccttttct gctgcagata cctcattacc tgccacagta gcgccagtcg
     3841 ttgcgatcat gcccagtgcc aaagttacag ttataaatga ttttaatttc atataatctc
     3901 tctcctttgt gagttaaagt atcttacaaa atcaatataa cacaaattga aaagtttcaa
     3961 aaattataag tatgtaaatt gtttacttgt ttttaaaaat aaattttaat taatgtaaaa
     4021 gctataattg ttaatacttt ctattttttc aaataaattc acattacaat aactaagttt
     4081 acatttctta aatttaatta cttctttgca ttatagcgaa cgccgtacct aatatataaa
     4141 ttaaaacctt tgtttctttg tacttttaat tgaacaattt tatattgatg agatatttag
     4201 tattaaattt ttcttcctat aaggttaata cttgtttgat caattttatg ttgatttact
     4261 tcttttaaaa at
//
LOCUS       DANJFZ010000166         4240 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.167, whole genome shotgun sequence.
ACCESSION   DANJFZ010000166 DANJFZ010000000
VERSION     DANJFZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.167"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            128..2950
                     /gene="fnbB"
                     /locus_tag="PWN45_002607"
     CDS             128..2950
                     /gene="fnbB"
                     /locus_tag="PWN45_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDL0624341.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            3130..3996
                     /gene="galU"
                     /locus_tag="PWN45_002608"
     CDS             3130..3996
                     /gene="galU"
                     /locus_tag="PWN45_002608"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0624342.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
BASE COUNT         1667 a          633 c          844 g         1096 t
ORIGIN      
        1 gttaacaaca atcttaactt tttattaact cgcttttttt cattgctttt aaaaaccgaa
       61 caatatagaa ttgcatttat tgagttttaa aaataaatga attttgcatt taagggagaa
      121 tattatagtg aaaagcaatc ttagatacgg cataagaaaa cacaaattgg gagcggcctc
      181 agtattctta ggaacaatga tcgttgttgg aatgggacaa gaaaaagaag ctgcagcatc
      241 ggaacaaaac aatactacag tagaggaaag tgggagttca gctactgaaa gtaaagcaag
      301 cgaaacacaa acaactacaa ataacgttaa tacaatagat gaaacacaat catacagcgc
      361 gacatcaact gagcaaccat cacaatcaac acaagtaaca acagaagaag caccgaaaac
      421 tgtgcaagca ccaaaagtag aaacttcgcg agttgatttg ccatcggaaa aagttgctga
      481 taaggaaact acaggaactc aagttgacat agctcaacca agtaacgtct cagaaattaa
      541 accaagaatg aaaagatcaa ctgacgttac agcagttgca gagaaagaag tagtggaaga
      601 aactaaagcg acaggtacag atgtaacaaa taaagtggaa gtagaagaag gtagtgaaat
      661 tgtaggacat aaacaagata cgaatgttgt aaatcctcat aacgcagaaa gagtaacctt
      721 gaaatataaa tggaaatttg gagaaggaat taaggcggga gattattttg atttcacatt
      781 aagcgataat gttgaaactc atggtatctc aacactgcgt aaagttccgg agataaaaag
      841 tacagatggt caagttatgg cgacaggaga aataattgga gaaagaaaag ttagatatac
      901 gtttaaagaa tatgtacaag aaaagaaaga tttaactgct gaattatctt taaatctatt
      961 tattgatcct acaacagtga cgcaaaaagg taaccaaaat gttgaagtta aattgggtga
     1021 gactacggtt agcaaaatat ttaatattca atatttaggt ggagttagag ataattgggg
     1081 agtaacagct aatggtcgaa ttgatacttt aaataaagta gatgggaaat ttagtcattt
     1141 tgcgtacatg aaacctaaca accagtcgtt aagctctgtg acagtaactg gtcaagtaac
     1201 taaaggaaat aaaccagggg ttaataatcc aacagttaag gtatataaac acattggttc
     1261 agacgattta gctgaaagcg tatatgcaaa gcttgatgat gtcagcaaat ttgaagatgt
     1321 gactgataat atgagtttag attttgatac taatggtggt tattctttaa actttaataa
     1381 tttagaccaa agtaaaaatt atgtaataaa atatgaaggg tattatgatt caaatgctag
     1441 caacttagaa tttcaaacac acctttttgg atattataac tattattata caagtaattt
     1501 aacttggaaa aatggcgttg cattttactc taataacgct caaggcgacg gcaaagataa
     1561 actaaaggaa cctattatag aacatagtac tcctatcgaa cttgaattta aatcagagcc
     1621 gccagtggag aagcatgaat tgactggtac aatcgaagaa agtaatgatt ctaagccaat
     1681 tgattttgaa tatcatacag ctgttgaagg tgcagaaggt catgcagaag gtaccattga
     1741 aactgaagaa gattctattc atgtagactt tgaagaatcg acacatgaaa attcaaaaca
     1801 tcatgctgat gttgttgaat atgaagaaga tacaaaccca ggtggtggtc aggttactac
     1861 tgagtctaac ctagttgaat ttgacgaaga ttctacaaaa ggtattgtaa ctggtgctgt
     1921 tagcgatcat acaacaattg aagatacgaa agaatatacg actgaaagta atctgattga
     1981 actagtagat gaactacctg aagaacatgg tcaagcgcaa ggaccaatcg aggaaattac
     2041 tgaaaacaat catcatattt ctcattctgg tttaggaact gaaaatggtc acggtaatta
     2101 tggcgtgatt gaagaaatcg aagaaaatag ccacgtggat attaagagtg aattaggtta
     2161 cgaaggtggc caaaatagcg gtaatcagtc atttgaggaa gacacagaag aagataaacc
     2221 gaaatatgaa caaggtggca atatcgtaga tatcgatttc gatagtgtac ctcaaattca
     2281 tggtcaaaat aatggtaacc aatcattcga agaagataca gagaaagaca aacctaagta
     2341 tgaacaaggt ggtaatatca ttgatatcga cttcgacagt gtgccacata ttcacggatt
     2401 caataagcac actgaaatta ttgaagaaga tacaaataaa gataaaccaa attatcaatt
     2461 cggtggacac aatagtgttg actttgaaga agatacactt ccacaagtaa gtggtcataa
     2521 tgaaggtcaa caaacgattg aagaagatac aacacctcca atcgtgccac caacgccacc
     2581 gacaccagaa gtaccaagcg agccggaaac accaacacca ccgacaccag aagtaccaag
     2641 cgagccggaa acaccaacac cgccaacgcc agaggtacca actgaacctg gtaaaccaat
     2701 accacctgct aaagaagaac ctaaaaaacc ttctaaacca gtggaacaag gtaaagtagt
     2761 aacacctgtt attgaaatca atgaaaaggt taaagcagtg gtaccaacta aaaaagcaca
     2821 atctaagaaa tctgaactac ctgaaacagg tggagaagaa tcaacaaaca acggcatgtt
     2881 gttcggcgga ttatttagca ttttaggttt agcgttatta cgcagaaata aaaagaatca
     2941 caaagcataa tcaatccaaa attgacaggt ttatttcata aattatatga agtaagcctg
     3001 ttttttaaaa ttaaaacaaa tttcccaaga aataattaca tactcaatga cactatgaag
     3061 gcgttctaat tagtgttaaa atgacgttga tacatagatt taatacttag gaaaaggagc
     3121 acattaactt tgaaaaaaat aaaaaaggca atcattcccg ctgctggttt agggactaga
     3181 tttttaccag caactaaagc gatgccaaag gaaatgcttc ctatcttaga taaacccaca
     3241 atacaatata tcgttgaaga agctgcaaga gctggaattg aagatattat tatagtgaca
     3301 ggtcgccaca aacgcgcgat tgaagatcat tttgatagtc aaaaagaatt agaaatggtg
     3361 ttaaaagaaa aaggtaaatc tgaattacta gagaaagttc agtattcaac ggaacttgcg
     3421 aatatttttt atgtaaggca gaaagaacaa aaaggtttag ggcatgcgat tagttctgcg
     3481 cgtcaattta tcggcaatga accatttgcg gtattattag gtgacgatat tgttgaatct
     3541 gaagtgccag cagtgaaaca gttgattgac gtttatgaag aaacagggca ctcagttata
     3601 ggtgtccaag aagttccgga agcagatact catcgttatg gaattattga cccattaact
     3661 aaaaacggac gtcagtatga agtgaaaaag tttgttgaaa agccagcaca aggtacggca
     3721 ccatcaaacc tagctattat gggacgttat gtattaacgc ctgaaatttt cgattattta
     3781 aaaacgcaaa aagaaggcgc aggtaacgaa attcagttaa cagatgcgat tgaacgtatg
     3841 aataatgata accaagtgta tgcatatgac ttcgaaggcg aacgttatga cgttggagaa
     3901 aaattaggct tcgttaaaac taccatagaa tatgcattga aagatgacag tatgcgagaa
     3961 gaattaacac gatttattaa agcattaggt ttataacatt aaaagcatga tactcagtaa
     4021 aattggaaca cgaaatggtg aactaaattg aatgagtgtc atgcttaatt tatgtgttgt
     4081 gatatatagt agggtattga aataatataa aaacaaaaga aagttaatta tgtattttta
     4141 tttatactaa attgagaagt agtatgattg cttatttatt ttatcaagag gtaaatctgt
     4201 ttaaatttaa aagaaaaatt ttcttggttt gagaatcttg
//
LOCUS       DANJFZ010000167         4145 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.168, whole genome shotgun sequence.
ACCESSION   DANJFZ010000167 DANJFZ010000000
VERSION     DANJFZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.168"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            417..1883
                     /gene="msr(A)"
                     /locus_tag="PWN45_002609"
     CDS             417..1883
                     /gene="msr(A)"
                     /locus_tag="PWN45_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDL0624343.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            1982..2881
                     /locus_tag="PWN45_002610"
     CDS             1982..2881
                     /locus_tag="PWN45_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL0624344.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3367..3975
                     /locus_tag="PWN45_002611"
     CDS             3367..3975
                     /locus_tag="PWN45_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0624345.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
BASE COUNT         1650 a          594 c          709 g         1192 t
ORIGIN      
        1 tattttatga gtacactcta aattaaggag atgtataatc aatctaaaat tgtattttta
       61 tacttttgtt atataatctt tctacttaga gatatattgt aaaaaaacca acaaaatgtt
      121 atattaacat tactatttta tcgatgaagg aggattaaaa tgacagcttc tatgagactc
      181 aaataaacct ttttgaatac aaaactaaat taattcaaaa aggcttgtcc gcaatacaca
      241 ctcataggat gacttatgtc attttttatc cttcgatagg aataataaac gctttcaaat
      301 actaaaacat cataataaat ttttaagtgt ttgaaagtgt aatgtgaatg agacgggagt
      361 agcctgtctc atttttttgc gtacacgtcc tttaaatata caaggagtgt ataaatatgg
      421 aacaatatac aattaaattt aaccaaatca atcataaatt gacagattta cgatcactta
      481 acatcggtca tctttacgct taccaatttg aaaaaatagc acttattggg ggtaatggta
      541 ctggcaaaac cacattacta aatatgattg ctcaaaaaac aaaaccagaa tctggaacag
      601 ttgaaacggt tggcgaaatt caatattttg aacagcttaa catggatgtg gaaaatgatt
      661 ttaacacgtt agacggtagt ttaatgagtg aactccatat acctatgcat acaaccgaca
      721 gtatgagtgg tggtgaaaaa gcaaaatata aattagctaa tgtcatatca aattatagtc
      781 cgatattact tttagatgaa cctacaaatc acttggataa aattggtaaa gattatctga
      841 aaaatatttt aaaatattac tatggtactt taattatagt aagtcatgat agagcactta
      901 tagaccaaat tgctgacaca atttgggata tacaagaaga tggcacaata agagtgttta
      961 aaggtaatta cacacagtat caaaatcaat atgaacaaga acagttagaa caacaacgtc
     1021 aatatgaaca gtatataagt gaaaaacaaa gattgtccca agccagtaaa gctaaacgaa
     1081 atcaagcgca acaaatggca caagcatcat caaaacaaaa aaacaaaagt atagcaccag
     1141 atcgtttaag tgcatcaaaa caaaaaggca cggttgagaa ggctgctcaa aaacaagcta
     1201 agcatattga aaaaagaatg gaacatttgg aagaagttga aaaaccacaa agttatcatg
     1261 aattcaattt cccacaaaat aaaatttatg atatccataa taattatcca atcattgcac
     1321 aaaatctaac attggttaaa ggaagtcaaa aactgctaac acaagtacga ttccaaatac
     1381 catatggcaa aaatatagcg ctcgtaggtg caaatggtgt aggtaagaca actttacttg
     1441 aagctattta ccaccaaata gagggaattg attgttctcc taaagtgcaa atggcatact
     1501 atcgtcaact tgcttatgaa gacatgcgtg acgtttcatt attgcaatat ttaatggatg
     1561 aaacggattc atcagaatca ttcagtagag ctattttaaa taacttgggt ttaaatgaag
     1621 cacttgatcg ttcttgtaat gttttgagtg gtggggaaag aacgaaatta tcgttagcag
     1681 tattattttc aacgaaagcg aatatgttaa ttttggatga accaactaat tttttagata
     1741 ttaaaacatt agaagcatta gaaatgttta tgaataaata tcctggaatc attttgttta
     1801 catcacatga tacaaggttt gttaaacatg tatcagataa aaaatgggaa ttaacaggac
     1861 aatctcttca tgatataact taaaaactat acactaattt atattgaata ttaggttcat
     1921 ttatatcaat ataaaaaata cgttcacaag aaaaatataa tagaaataag gatccaataa
     1981 aatgactcga cataatgaaa ttattaaatg tgcagaaaaa tatcaattac acatccaacc
     2041 tcaaacaatc tcattgaatg aatcgggact tgattttcaa gttgcatttg gaaaagataa
     2101 acatggagta gaatgggttt tgagactacc aagaagacct gacgtttata aacgaacaaa
     2161 acccgaaaaa caaacggtag acttcttaca gaagaatgtt tcatttgaaa taccgaagtg
     2221 gaaagtacat gcaaaagacc ttattgctta cccaaaactt acaggtaaac ccgcagccac
     2281 aatagatcca gaaatacaaa attatgtatg ggaaattgaa cacaaaccat taccagaaaa
     2341 ctttattaac acattagctg aaacactcgt agatttacac aacataccag aagaaaacat
     2401 taacgttcag catataaata tcaaaaccat acaagaaata aaaaatgact ttcaaagaag
     2461 aatgaataaa gttaaagaaa cttatggcgt atcagatgaa ttatggaaca gatggaaaca
     2521 atggttagaa aacgacgaac tatggcctcg acatgcgacc atgatacatg gggacttaca
     2581 tccaggacat ataatggtag ataaccaagc aaacgtcaca ggtctcatag actggactga
     2641 agcaacccac tccgacccat caatggactt tataggacac catcgtgtat tcgacgacga
     2701 aggattagag caacttataa cagcatacgg taaagctgga ggtgaaatat ggccacgaat
     2761 gaaagagcat ataatagaac tcaatgcagt attcccaatg tttatcgctg agtttgctat
     2821 ggaatcagga gaatcggcgt atgaaacgat ggcattgaaa gagttaggta tgaaagagta
     2881 gaacttatat taaaagaggc ccattttaaa gacgaaggca atcctgatga aattttaaaa
     2941 ctgcgtttaa ttagtgatgg tctttggttt tctataatgt attaaatatc tggacatgga
     3001 acaaatgcaa caaatggaaa gaatcgtttt cgaaatttct aactccttaa atcaggagga
     3061 taaataaacg gtcattttca ttgaaagcac ttatataaat ttcatcaatt ttaataccta
     3121 ccgtaaatac atttcataag tattaaacag atgattaaaa tttttagtta atttaaatat
     3181 ttatgataga gatgaccttc aaatgaacgg ttctgttaca aagaaaaaat ctatatttat
     3241 ttttagtaag ttttaattat agatatgtac actttgagta caccctaacc atacaaaaag
     3301 aattgttgaa attataacaa ttaaaacgta tgattagggt gtatatttat tatggagtga
     3361 agatgaatga ttataggtta tgcgagagta tcatcgatag atcaaaattt agaaagacaa
     3421 ttggataatt taaagacgtt tggtgtggag aaaatcttta cagagaaaca gtcgggaaag
     3481 tcagtggaaa atagacctgt atttcaagaa gcactcaatt ttgtcagaat gggagatcga
     3541 ttcgttgtgg aatctattga tcgtttagga cgtaactatg atgaaattat tgaaactgtg
     3601 aattatttaa aagaaaaaga cgttcagtta atgattacca gcttacctat gatgaatgaa
     3661 gtaattggca atccattact ggataagttt atgaaagatt taatcattca aatattagca
     3721 atggtttcag aacaagagag aaatgaaagt aaacgtcgac aagcgcaagg gattcaagtt
     3781 gcgaaggaaa aaggcgtata taaagggcgc cctttacttt attcaccaaa tgctaaagac
     3841 ccacagaaac gtgttattta tcatcgcgtg gtggaaatgt tagaagaagg caaagcgatt
     3901 agtaagattg cgaaagaagt taatatcaca agacaaacag tttatagaat taaacatgat
     3961 aagggattat cttgataaat tgattgtcaa tgcaaatgag cgtctctcac agtgtctcaa
     4021 attccataac atattattga attgagattt taataattat acaagaaaaa agagtacgag
     4081 ttcgcagctt tctatatagt aattgtttta ctaataaggt gttttaaaat atattataat
     4141 gtaaa
//
LOCUS       DANJFZ010000168         3966 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.170, whole genome shotgun sequence.
ACCESSION   DANJFZ010000168 DANJFZ010000000
VERSION     DANJFZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.170"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            71..1432
                     /locus_tag="PWN45_002612"
     CDS             71..1432
                     /locus_tag="PWN45_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0624346.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKRRQPSPIMDWPPNNKNSKSCILK
                     IVL"
     gene            1408..1521
                     /locus_tag="PWN45_002613"
     CDS             1408..1521
                     /locus_tag="PWN45_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624347.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(1525..1608)
                     /locus_tag="PWN45_002614"
     tRNA            complement(1525..1608)
                     /locus_tag="PWN45_002614"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1572..1574),aa:Leu,seq:caa)
     gene            complement(1647..1721)
                     /locus_tag="PWN45_002615"
     tRNA            complement(1647..1721)
                     /locus_tag="PWN45_002615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1686..1688),aa:Gly,seq:tcc)
     gene            complement(1825..1899)
                     /locus_tag="PWN45_002616"
     tRNA            complement(1825..1899)
                     /locus_tag="PWN45_002616"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Gly,seq:tcc)
     gene            complement(1907..1980)
                     /locus_tag="PWN45_002617"
     tRNA            complement(1907..1980)
                     /locus_tag="PWN45_002617"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Cys,seq:gca)
     gene            complement(1986..2057)
                     /locus_tag="PWN45_002618"
     tRNA            complement(1986..2057)
                     /locus_tag="PWN45_002618"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2023..2025),aa:Gln,seq:ttg)
     gene            complement(2069..2141)
                     /locus_tag="PWN45_002619"
     tRNA            complement(2069..2141)
                     /locus_tag="PWN45_002619"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2106..2108),aa:His,seq:gtg)
     gene            complement(2144..2217)
                     /locus_tag="PWN45_002620"
     tRNA            complement(2144..2217)
                     /locus_tag="PWN45_002620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2183..2185),aa:Trp,seq:cca)
     gene            complement(2233..2313)
                     /locus_tag="PWN45_002621"
     tRNA            complement(2233..2313)
                     /locus_tag="PWN45_002621"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Tyr,seq:gta)
     gene            complement(2319..2394)
                     /locus_tag="PWN45_002622"
     tRNA            complement(2319..2394)
                     /locus_tag="PWN45_002622"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2359..2361),aa:Thr,seq:tgt)
     gene            complement(2402..2474)
                     /locus_tag="PWN45_002623"
     tRNA            complement(2402..2474)
                     /locus_tag="PWN45_002623"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2439..2441),aa:Phe,seq:gaa)
     gene            complement(2493..2568)
                     /locus_tag="PWN45_002624"
     tRNA            complement(2493..2568)
                     /locus_tag="PWN45_002624"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2533..2535),aa:Asp,seq:gtc)
     gene            complement(2578..2651)
                     /locus_tag="PWN45_002625"
     tRNA            complement(2578..2651)
                     /locus_tag="PWN45_002625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Met,seq:cat)
     gene            complement(2666..2758)
                     /locus_tag="PWN45_002626"
     tRNA            complement(2666..2758)
                     /locus_tag="PWN45_002626"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2720..2722),aa:Ser,seq:tga)
     gene            complement(2797..2872)
                     /locus_tag="PWN45_002627"
     tRNA            complement(2797..2872)
                     /locus_tag="PWN45_002627"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2837..2839),aa:Asp,seq:gtc)
     gene            complement(2883..2972)
                     /locus_tag="PWN45_002628"
     tRNA            complement(2883..2972)
                     /locus_tag="PWN45_002628"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2934..2936),aa:Ser,seq:tga)
     gene            complement(2982..3055)
                     /locus_tag="PWN45_002629"
     tRNA            complement(2982..3055)
                     /locus_tag="PWN45_002629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3019..3021),aa:Ile,seq:cat)
     gene            complement(3084..3157)
                     /locus_tag="PWN45_002630"
     tRNA            complement(3084..3157)
                     /locus_tag="PWN45_002630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3121..3123),aa:Met,seq:cat)
     gene            complement(3179..3254)
                     /locus_tag="PWN45_002631"
     tRNA            complement(3179..3254)
                     /locus_tag="PWN45_002631"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3219..3221),aa:Ala,seq:tgc)
     gene            complement(3272..3345)
                     /locus_tag="PWN45_002632"
     tRNA            complement(3272..3345)
                     /locus_tag="PWN45_002632"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3309..3311),aa:Pro,seq:tgg)
     gene            complement(3355..3428)
                     /locus_tag="PWN45_002633"
     tRNA            complement(3355..3428)
                     /locus_tag="PWN45_002633"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3392..3394),aa:Arg,seq:acg)
     gene            complement(3447..3535)
                     /locus_tag="PWN45_002634"
     tRNA            complement(3447..3535)
                     /locus_tag="PWN45_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3499..3501),aa:Leu,seq:taa)
     gene            complement(3546..3620)
                     /locus_tag="PWN45_002635"
     tRNA            complement(3546..3620)
                     /locus_tag="PWN45_002635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3586..3588),aa:Gly,seq:gcc)
     gene            complement(3624..3705)
                     /locus_tag="PWN45_002636"
     tRNA            complement(3624..3705)
                     /locus_tag="PWN45_002636"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3669..3671),aa:Leu,seq:tag)
     gene            complement(3709..3784)
                     /locus_tag="PWN45_002637"
     tRNA            complement(3709..3784)
                     /locus_tag="PWN45_002637"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3749..3751),aa:Lys,seq:ttt)
     gene            complement(3790..3865)
                     /locus_tag="PWN45_002638"
     tRNA            complement(3790..3865)
                     /locus_tag="PWN45_002638"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Thr,seq:tgt)
     gene            complement(3882..3957)
                     /locus_tag="PWN45_002639"
     tRNA            complement(3882..3957)
                     /locus_tag="PWN45_002639"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3922..3924),aa:Val,seq:tac)
BASE COUNT         1255 a          868 c          832 g         1011 t
ORIGIN      
        1 tacacaaaaa gcgcaattgc ctctataatt taaagtgacc aaacccaaac taaaggagac
       61 aagtgcgcct atgtgtaatg ataccttaga attactaaga ataaaagatg aaaatataaa
      121 atatataaac caagaaattg acgtcattat caaaggaaaa aaagcaacag tggttaatgc
      181 tgtactaacg tataagcctt cggcctgtta ttgttgtgga gttaaaaatg aaggacaaat
      241 tcataaacat ggtaagcgtg tttctcgtat tactttactt aaaactcaag ggtataacac
      301 atacctcaac ttagctaaac aacgttttaa atgcttagaa tgcaatggca cttttactgc
      361 taaaacgtca attgttgatg agtcgtgttt tatctcaaga tgtgttactc aaaaagttat
      421 agaagaagct actaaagtta aaacagagat tgatactgca gaagataact gtatctctcc
      481 atctactgta agtcgtatta gaactaaagc ggctaattca ttacgaatta aaccctttaa
      541 ttgtttgcca gaacacatcg ctatggatga atttaaaagc gttaaaaatg taactggatc
      601 aatgagtttc atttttatag ataatgatac tcatgatgtt atagatattt tagaaaatag
      661 aactacaaga ttcttgcgtg cctatttcga gcgattcgat ttaaaaaatc gacaacaagt
      721 taagacggtt actattgaca tgtatgaacc ctatgtccga ttatttcgcg acctatttcc
      781 taatgcagct attatttttg acagattcca tatcgttcaa catttaaata gagaacttaa
      841 taagtatcgt gtacaagtta tgaatgaata ccgtaataaa aaaggacctg attatacaat
      901 ttttaagaat aactggaaag tcctattgat ggatactagt aaaaccatat ttagtaaata
      961 cagatggaat aaatctttta aggcttataa acgctcatct gacattgtag aattcatgct
     1021 ttcaaaagac gatatactac gacactccta cgaacttgtc caaggattac gaaaagacct
     1081 aaggttatgt aattggccta aatttattaa tcgtttaaat tcagttagta aaaagtctgt
     1141 gagtaagggt gtatggaaag cggttaaata ttatagaaaa catcaaagga tgttaagaaa
     1201 tacaatttat tacccagcat ttaataatgg tgctatagaa ggaattaata ataagataaa
     1261 attaatcaag cgaatttctt ttggttacag aaatttcaac aactttaaag cacgtataat
     1321 gatgattttc agcttgtaca aaggagaaaa aagaagacaa ccaagcccaa taatggactg
     1381 gccgcctaat aataaaaact ctaaaagttg tattttaaaa atagttcttt aaattatata
     1441 cccaccacat ttggtggaga accaaaagaa accttgtttt acaaggtttc taatacgtta
     1501 tgttatgtaa ataacagtta attataccgg tggtcggggt cgaaccgaca ctccacaagt
     1561 ggaacgggat tttgagtccc gcgcgtctgc caattccgcc acaccggctt aatggtaaac
     1621 aaaaaacttc cctttggaag caattatgga gcggaagata ggatttacac ctatacctcg
     1681 ttccgggaag gaacgtgttc taaaagttga actactcccg caaatattaa attatggagc
     1741 ggaagatagg atttacacct atacctcatt ccaggaagga atgtattcta agagttgaaa
     1801 tactcccgca ttattattaa attatggagc ggaagatagg atttgcacct atacctcgtt
     1861 ccgggaagga acgtgttcta aaagttgaac tactcccgca taaacctgga ggcggcaacc
     1921 ggatttgaac cggtgataaa ggttttgcag acctctgcct taccacttgg ctatgccgcc
     1981 aataactggg ctagctggat tcgaaccaac gagtgacgga gtcaaagtcc gttgccttac
     2041 cgcttggcta tagcccatta ataataaggg cggctgaagg ggatcgaacc ctcgaatgtc
     2101 ggaaccacaa tccgatgtgt taaccacttc accacagccg ccatggcagg ggcagtagga
     2161 atcgaaccca caccaaaggt tttggagacc tctattctac cgttgaacta tgcccctatt
     2221 aaaaataata aatggagggg ggcagattcg aactgccgaa cccgaaggag cggatttaca
     2281 gtccgccgcg tttagccact tcgctacccc tccataaatg gtgccggcca gaggacttga
     2341 acccccaacc tactgattac aagtcagttg ctctaccaat tgagctaggc cggctaagaa
     2401 atggttcagg acagagtcga actgccgaca catggagctt caatccattg ctctaccaac
     2461 tgagctactg aaccataata aaaatgtaat gatggcggtc tcgacgggaa tcgaacccgc
     2521 gatctcctgc gtgacaggca ggcgtgttaa ccgctacact acgagaccta taaaatattg
     2581 cgggaggcgg atttgaacca ccgaccttcg ggttatgagc ccgacgagct accgaactgc
     2641 tccatcccgc gataataaaa aataatggcg gaggaagagg gattcgaacc cccgcggccc
     2701 gttaaggccc tgtcggtttt caagaccgat cccttcagcc ggacttgggt attcctccat
     2761 tattataggt aaatcgctat taattataaa attaaatggc ggtctcgacg ggaatcgaac
     2821 ccgcgatctc ctgcgtgaca ggcaggcgtg ttaaccgcta cactacgaga ccattagtaa
     2881 aacggaggaa gagggattcg aacccccgcg agccgttaag cccctgtcgg ttttcaagac
     2941 cgatcccttc agccggactt gggtattcct ccaaaattat atggaccttg caggactcga
     3001 acctgcgacc gaacggttat gagccgttag ctctaaccaa ctgagctaaa ggtcctaaat
     3061 ataattttac aactaataaa tagtggcggt ggaggggatc gaacccccga cctcacgggt
     3121 atgaaccgta cgctctagcc agctgagcta caccgcctta tatagtttgt aaataatatg
     3181 gtggagacta gcgggatcga accgctgacc tcctgcgtgc aaagcaggcg ctctcccagc
     3241 tgagctaagc ccccataata attacagtat atcgggaaga caggattcga acctgcgacc
     3301 ccttggtccc aaaccaagtg ctctaccaag ctgagctact tcccgtataa ttaacgcgcc
     3361 cgataggagt cgaacccata acctcttgat ccgtagtcaa acgctctatc caattgagct
     3421 acgggcgcat atgtttttat tgaaaatggt gccgaggacc ggaatcgaac cggtacggtg
     3481 atcactcacc gcaggatttt aagtcctgtg cgtctgccag ttccgccacc ccggcactat
     3541 aaaaatggag cagaagacgg gattcgaacc cgcgacccca accttggcaa ggttgtattc
     3601 taccgctgaa ctacttctgc atatgcgggt gaagggagtc gaacccccac gccgtaaggc
     3661 gctagatcct aagtctagtg cgtctgccaa ttccgccaca cccgcaaatg gtgagccata
     3721 gaggattcga acctctgacc ctctgattaa aagtcagatg ctctaccaac tgagctaatg
     3781 gctcttccat ggtgccggcc agaggacttg aacccccaac ctactgatta caagtcagtt
     3841 gctctaccaa ttgagctagg ccggcaatat gtaagaataa atggtggaga atgacgggtt
     3901 cgaaccgccg accctctgct tgtaaggcag atgctctccc agctgagcta attctccgat
     3961 ttaaaa
//
LOCUS       DANJFZ010000169         3890 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.171, whole genome shotgun sequence.
ACCESSION   DANJFZ010000169 DANJFZ010000000
VERSION     DANJFZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.171"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            261..1034
                     /locus_tag="PWN45_002640"
     CDS             261..1034
                     /locus_tag="PWN45_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0624348.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            1146..1930
                     /locus_tag="PWN45_002641"
                     /pseudo
     CDS             1146..1930
                     /locus_tag="PWN45_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1927..3251
                     /locus_tag="PWN45_002642"
                     /pseudo
     CDS             1927..3251
                     /locus_tag="PWN45_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3251..3577
                     /locus_tag="PWN45_002643"
     CDS             3251..3577
                     /locus_tag="PWN45_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624349.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            3580..3867
                     /locus_tag="PWN45_002644"
     CDS             3580..3867
                     /locus_tag="PWN45_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624350.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT         1616 a          491 c          714 g         1069 t
ORIGIN      
        1 attttaataa gtaaatacat tgagtttcag tgttattatt ttaaatataa gcgcaattta
       61 tcttaattaa ttagttggtt aggccaataa aaattataaa gatatttgcg ctttttaaat
      121 aatttgtgat gttgatatgt gaaaaataca actaacaatt aaaatccacg aggatttaat
      181 cataaaattt cctctaatat tattaatatt ggaatttgaa cttaactttg catcaaaaat
      241 aaaaatgggg atgtgaaatg atgattcact caaaaaggtt gaggctctgg ctgtatttag
      301 tattattagc tgtttttata ggggcttgcg gaatgaaaaa ggaagaaagc agtaaagata
      361 aacaaattaa agaaaacttc aacaaaacgt tgagcctgta tccaactaaa aatcttgaag
      421 acttttatga taaggaaggc tttcgagatc aagagtttga aaagggcgac aaaggcactt
      481 ggataattca ctctaaaatg acaatagaaa caaatggaaa gaatatggaa tccagaggat
      541 tggtcctgta tgtagaccgc aataccagaa cgacaaaagg tgaatttatt gtgagggaat
      601 tatgggaaga taaaaaagga tactcacgta gtaaagaaaa agaatatcca gtaaaaatgg
      661 aacataataa aattatccca accaaaccga tagctgatga taaattaaga aaagaaatcg
      721 aaaactttaa attctttgta caatacggtg actttaaaga tattaatgat tataaagatg
      781 gtgacatttc atataatccg aatgtgccaa gttattcagc aaagtatcaa ttaagtaatg
      841 atgactacaa tgtgaagcaa cttagaaaga ggtatgatat accaactaag aaagcaccta
      901 aattattaat aaaaggcgat ggtgatttaa aaggatcatc tataggacac aaaaatttag
      961 agtttacatt tatagaaaat aaagaagaaa acatctattt tacggatagt attaatttca
     1021 aacctactga atagggttaa tagaagtttg aaatgaaaat ttcataaagt gttcaaaata
     1081 atttttcgct ttagttgtgt aaattaaaga ttgagtcagt attaaaaata taaggtggag
     1141 aaatgatgat tcattcaaag aaattaacgc tcggtatatg tttggtatta ctcattatat
     1201 tgattggagg ttgtgtcatt atgacaaaaa caaatggtcg aaacgctcaa attaaagaaa
     1261 atttcaacaa aacattaagt gtatatccaa ccaagaatct cgatgatttt tacgataagg
     1321 aaggttttcg agatcaagaa tttgataaaa gagataaagg gacttggatt atttattctg
     1381 aaatggttat cgaaccaaaa gggaaaaata tggaatcgag aggaatggtg ctctatatca
     1441 atcgcaatac tagaacaacg aagggtaatt ttattgtcac cgaaataact gaagatagta
     1501 aaggatattc acgtagtaaa gaaaaaaata tcctgtcaag atggaaaata atcgaattat
     1561 tccaacaaag cctataccgg atgacaagtt aaaaaaagag attgaaaact ttaagttctt
     1621 tgtacaatat ggaaatttta aagattttaa agattataaa aatggtgata tttcatataa
     1681 tcctaatgtg ccaagttatt ctgcaaagta tcaattgaat aatgatgact ataatgttca
     1741 acagttaaga aaacgatatc atattccaac caaacaagcg cccgaattaa aattgaaagg
     1801 atccggcaat ttaaaaggct catccgtagg atctaaggat ctagaattta cgtttgtaga
     1861 aaatcaagaa gagaatatct atttttcaga ttcggtcgaa tttacaccta gcgaggatga
     1921 taaatcatga gtcaaacgga atatcaaata aaatctggca atataaaagg taactctgaa
     1981 gaaacaagta cagtatctaa tataagttat gaaatagaaa acgcaaataa cagtggttta
     2041 aaacaaaata aaattgataa acaaattaaa aagttacaag aaaaaaataa attccctaaa
     2101 aatctttcat atcttaaaag ttatacggac cccaaaacag gcacgactac aagcgccttt
     2161 ttaaataaag acactggcaa agttacttta ggtatgacag gtactaatgt acacaaagac
     2221 gcaatattaa aacaaacatt tggtgttcct tcttatcaag gatatataga tgtgagtgaa
     2281 acgctaaagg atattggggc cgatgtcaat attggccttc attccgtcac agataaagat
     2341 ccacattata aaaataccca agactttatc aaaaatatca aaaaagacta tgatattgat
     2401 attattaccg gacattcgct gggcggtaga gatgcgatga ttttaggtat gagtaatgat
     2461 attaaacata tcgttgtgta taatccagct ccattagcga tcaaagatgt gagcggcctt
     2521 tatgccgatc aagaagaact taaaaaattg attgaaaagt acgatggtca cattgtaaga
     2581 tttgtgtctg atgaagacga attagatgca ggtgtccgca atcatttata tgaaactgct
     2641 ggagaaaaaa tagtacttaa aaatggagaa ggccatgcaa tgagtggtat tttaatgagc
     2701 agaacacagg ctataatctt agctgaatta aacaaagtta aaggctacca agacgaaaat
     2761 aataaagcat taaaatccgt tcgtaaacaa acgaggcata gattacataa agtagagacg
     2821 ttaagagcga attggattca aacaacgggt ggatcactct cttcctccca acaacaatta
     2881 ttagaagctt taacagcact aaccattgcc gaaggcttaa atcaattagt gaatgaagaa
     2941 agccaacatt tgaaaaaatg tatcacgcga tggcacataa atttggagac aactggaaaa
     3001 aagcgcaaga agttggaaat gaaattggtg aaaaattaac ctctgaagag gttatagatg
     3061 aattaagaaa aggtggcgcg tatgaaagta aacttgaaac agatcccaaa agaaaaattg
     3121 atgataagat aaagaaatta aatgatgttt ataaaaattg taatggctat atcgcaaaaa
     3181 ttaaacagag tatcgaagca attgtttcta atgaccaaat gttagcgagc cagattgatg
     3241 ggatgatgta atgtttacta cgtataaaaa tattaatgaa cttgaaaatg cctatgatga
     3301 agaaagaaaa caattgaatg atgcattcaa tcaaattgat gaattaagac atcaaacacg
     3361 caagaaatgt gaacaaatgt atgatcattt cttatatctc aaacataaaa tgaattatag
     3421 tgaagacgct atgatcagga tgacacgtat tatagaatct ttcgatagag aaacgaatca
     3481 acgtatccga catcacgaaa tgaaattaga agattataaa gatgagttaa gaagagaata
     3541 tctaaaacaa tctgacagaa ttgaaggaga tgaataagcg tgggacttga ttttagtggt
     3601 ttaccagatt tagcagtatt ggaacaaatg aaggaaaaag aacagattag tgaggttatt
     3661 gcgcctgaac atgttcgtat gcatcatgat catcaaaata agctgaaaag tgatgagaaa
     3721 atattacttg accaaatggt aagtcatttc aaaaaatttg aagatgattt taaaaatgcg
     3781 gcacaagggg cttgggtgaa aaatgccaca gacgaattaa aagatattag taatgattta
     3841 gaaaaaattc aagatattaa agtataaaaa ggtattaaga aacacaataa
//
LOCUS       DANJFZ010000170         3879 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.172, whole genome shotgun sequence.
ACCESSION   DANJFZ010000170 DANJFZ010000000
VERSION     DANJFZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.172"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..297
                     /locus_tag="PWN45_002645"
     CDS             <1..297
                     /locus_tag="PWN45_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0624351.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="PWN45_002646"
                     /pseudo
     CDS             355..732
                     /locus_tag="PWN45_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="PWN45_002647"
                     /pseudo
     CDS             729..1610
                     /locus_tag="PWN45_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="PWN45_002648"
     CDS             1726..2655
                     /locus_tag="PWN45_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624352.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="PWN45_002649"
     CDS             2648..3385
                     /locus_tag="PWN45_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624353.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..3602
                     /locus_tag="PWN45_002650"
     CDS             3399..3602
                     /locus_tag="PWN45_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624354.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
     gene            3632..>3879
                     /locus_tag="PWN45_002651"
     CDS             3632..>3879
                     /locus_tag="PWN45_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0624355.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKI"
BASE COUNT         1422 a          532 c          685 g         1240 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt taggaggttc tgttgcaaag ttgaatttat
     3601 aatataattt taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa
     3661 caaggatgtt atcactgtag ccgttggcta ctatctaaga tatgcattga gctatcgtga
     3721 tatatctgaa atattaagag aacgtggtgt aaacgttcat cattcaacga tctaccgttg
     3781 ggttcaagaa tatgccccaa ttttatatca aatttggaag aaaaagcata aaaaagctta
     3841 ttacaaatgg cgtattgatg agacgtatat caaaataaa
//
LOCUS       DANJFZ010000171         3756 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.173, whole genome shotgun sequence.
ACCESSION   DANJFZ010000171 DANJFZ010000000
VERSION     DANJFZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.173"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            121..591
                     /gene="sigS"
                     /locus_tag="PWN45_002652"
     CDS             121..591
                     /gene="sigS"
                     /locus_tag="PWN45_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDL0624356.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            704..1147
                     /locus_tag="PWN45_002653"
     CDS             704..1147
                     /locus_tag="PWN45_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDL0624357.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(1134..1577)
                     /locus_tag="PWN45_002654"
     CDS             complement(1134..1577)
                     /locus_tag="PWN45_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624358.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            1873..2508
                     /gene="sdpA"
                     /locus_tag="PWN45_002655"
     CDS             1873..2508
                     /gene="sdpA"
                     /locus_tag="PWN45_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDL0624359.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(2948..3661)
                     /locus_tag="PWN45_002656"
     CDS             complement(2948..3661)
                     /locus_tag="PWN45_002656"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL0624360.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT         1287 a          610 c          459 g         1400 t
ORIGIN      
        1 acttatgtct tatatttaac acttaattta gcgattacgc aaatgaatga tttttctaaa
       61 tattttattc taactttatc cggaaatatt tgttatacgt ataacttgga gaatgactac
      121 ttgaaattta atgacgtata caacaaacac cacaaaatca tacaccatct tttaaaaaaa
      181 tataatatta gctataatta tgatgagtat tatcaactac tcttgataaa aatgtggcaa
      241 ttgagtcaga tatataaacc ctcaagcaag caatctttat cctctttttt attcactcga
      301 ttaaattttt accttatcga tttattcaga caacaaaatc aattaaaaga tgtcatttta
      361 tgtgagaata attcaccaac attaactgaa caaccaacat actttaatga acatgacctt
      421 cgtttacaag atatcttcaa gcttttaaat caaagagaaa gactatggct caaactatac
      481 cttgaaggat acaagcaatt tgaaattgct gaaatcatgt cattatcgct ttcaacgatt
      541 aaattaatta agatgtccgt taagcgtaaa tgccaacata attttaatta gtttattgtg
      601 aaatttcggt cgatgtgtta atgatgttga aacaccctaa catcttcata taaatttatt
      661 ttaaccatta aattaacaac ttattaaacg aggagtacta tatttgcaag acaattctac
      721 taaatatcta ctttatatcc aaactgctac ttcaaaccat ctcgaaacaa attgtgtctt
      781 tttacattgc gattacattc ttaaagttcc aattaacaaa ctcgtttcat attatgcgaa
      841 attgcattta tcatcacaaa gtgtgctaat tgagactgca aaaaacatac taaatattaa
      901 taaactggtt cctatttaca tcaacccaaa aactatactt tttccgttaa aacataaacg
      961 tgcaccgata caaatttata tcaatgcaca ttatattgtt ggtatgactg ccatagagaa
     1021 ttcaacatta atacattttc aagaaggcat tcagctagaa gttgatgagc cattttctct
     1081 agtttcaaaa aaatgtcatg aaagtttagc tttgaagcac ttcattgaaa atactatttc
     1141 gaattaaatt taatttctaa aagtttctta gtaaagtatg atgtcacaat ttttataaaa
     1201 tatatgctag caaacattaa taaaatcgct ccaataattg ttaaaataac aacagttgta
     1261 gataaatcta ccttaggact aacgaaaaat tgtacgccag atagacttgt aattagccca
     1321 aataaaaaca aacctaataa agcaacataa gcaaacatca tcactactaa tgtaacaaac
     1381 atactataac tttttgtttc tttatttcga aatacatttt gtcttaagta agcattagca
     1441 aaggtaacag gcttatttaa gttaacgctg tcatcatcag atatagaaga caatcgttga
     1501 tctaatgctt gtttctcctt cttgttcaaa aaccataatt gctttttaac ttttttatca
     1561 aatgatgctt tattcataat taaggatttc tctcctttat aatattgacg taattttaaa
     1621 cattagcgtc aattgaaagt gtattttata tattttacag aaatatatgt atcaaatcta
     1681 caaaaatcca tattacaatt tcatttatgg tgatttgacg ctaaaataca gtctgctaat
     1741 ttttcaaact aatgcttttt aggtaggtac aatgataaat tttaatcgta aatatgtttc
     1801 atatattatg aatctagcct taatcatatt gcattcaaaa taattttaaa aaatgaaaag
     1861 aaaggattta gcatgcaaaa attcaaagac tttttttacg atgatttatc ggttacacga
     1921 ggaaattatt ttttaacttt aatggcagca ttttttatta ctatcatttt atttatcggc
     1981 atagttgtca gtgaagtaca tttactttat agcatgctaa ttgtattagt aggtttaatt
     2041 ctattgaggc tattcaaaat caatttattc tcttttaaaa aattaacatt gtctcaagtt
     2101 atttatatta taggcggtgc actattaatt tatgggttag ataatcttta tttatatttt
     2161 catgacgtac cggcaaatga acaacaatta gagcaagcaa tacgaaatac accattctat
     2221 atttctattt tcactgttac catcatcccc gctattgtgg aagaaattgt ttttcgcggt
     2281 atgataataa gggttatctt cagaaaacac ttgtttttag ggttaattgt gtctagttta
     2341 gtttttgcat cattacacga atctgacact tggattggtt atttacctta cttatattct
     2401 ggtttgattt ttggcctaat ttatataaaa acaaaacgat tagaagttgt aatattcatg
     2461 cacttcttaa ataacttgtt agctctgctc tttataatat ggagataatt ttgagataac
     2521 ctttcatatt attttcaaat tatctatctg atatttattt tattgaactt gagtcaccac
     2581 ttaaactcaa aaatacgtgt catcaagtgc aaaataattt agaactaata caaacataaa
     2641 aacttttact ttacgcttaa aatcaaaagt cacataccta gatataagta caacttgtat
     2701 attttgtact ctaataggta tgtggcttaa ataatattta attcttgagt tgcatatagt
     2761 aactgttttt attcattttc taaaatatgc aagactaatt tccttttcta ctcctgttga
     2821 atcaatttag acttttttat aactcgagct tttacttagc tgaattttga cttcttcttc
     2881 attaataact atgatggaaa aacccccata agcacaatgc ctatgagggt ttaaatatat
     2941 ttatatatta tagaatagaa agacctgaag attgaatatc tttcgcaaag cctttaactg
     3001 tatctactga taattcgtta atatcgcgac ctaagtttgt attcactttt ttcacaacat
     3061 ctgctgggca tgtaataata tctgcaccaa tttcatcagc ttgaatcaca ttgaataatt
     3121 cgcggcaact tgcccataat aatttaacgc cgtctttact atgcgtaact tttacagcct
     3181 ctttcattaa tggtaatgga tctacgcctg tatctgcaat acgtcctgca aatactgaaa
     3241 catatgttgg cacaccttca gttactgctt cagttatttc tttaacttgt tcaattgtgt
     3301 aaacagccgt aacgtttaat ctcacattgt cagctgaaag ttttttaatt aaaggaatcg
     3361 ttgattcacc ttttgtattt acaataggaa ttttaacaaa tacattttcg ccatattgtt
     3421 ttaaaattgc tgcttctttt tccatagttt ctaaatcgtc tgcaaatact tcaaatgaaa
     3481 ttgaagcatc tggaatttct ttcacagctt cttcagcaaa agctttgtaa tctgttacgc
     3541 ccgctttagc cattaaacta ggatttgttg taaaaccatc cacttgtttg tttttataag
     3601 ctgctttcat ttcttcaata tctgcaccgt ccgcaaatac ttctatattt agtttagcca
     3661 tataatatag cctccttgat tcttattaaa attttaacaa catctgcatg tctttttctt
     3721 acaaccattt gtaaaaaatg atttttattt ctttgt
//
LOCUS       DANJFZ010000172         3752 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.174, whole genome shotgun sequence.
ACCESSION   DANJFZ010000172 DANJFZ010000000
VERSION     DANJFZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.174"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            206..1171
                     /locus_tag="PWN45_002657"
     CDS             206..1171
                     /locus_tag="PWN45_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0624361.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            1268..1459
                     /locus_tag="PWN45_002658"
     CDS             1268..1459
                     /locus_tag="PWN45_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624362.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            1551..1880
                     /locus_tag="PWN45_002659"
     CDS             1551..1880
                     /locus_tag="PWN45_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624363.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(1957..2316)
                     /locus_tag="PWN45_002660"
     CDS             complement(1957..2316)
                     /locus_tag="PWN45_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624364.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIAL
                     ILYILNINRGIKILLKK"
     gene            complement(2846..3696)
                     /locus_tag="PWN45_002661"
                     /pseudo
     CDS             complement(2846..3696)
                     /locus_tag="PWN45_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1403 a          474 c          565 g         1310 t
ORIGIN      
        1 tgttaaagat catcaattat tttgtcacat tatcttgaga ggattacctt atgaggtagc
       61 cttttatttg tcacctaata ttgctctaag gctaataata catatataaa ataaataatt
      121 atcagaatat atttagaaat tgatatttaa taattaaata aaagtatgct aaatatttat
      181 aatttacaag tgagctgata gaaagatgag tataagcgaa tataaaaata gatacttaga
      241 gtcactatat aacaccttta aaactgagtt agaaaacgaa cgcactcgct attttatatt
      301 agataccaac tatacaaata gtgatgatac tgaaaaggag tttacttggg atgtaaaaca
      361 gaataataag atcagagaag gcgatttgtt catttttaga cggccaacta atttatctca
      421 aattgctcaa caattttatt tctttggagc tgggaaaata gaaaaaattg agcgaaaaaa
      481 taatgtagca accgcttaca tttcaaaacc cttacttttt gtggatcgtg ttttaaaaga
      541 taatattgaa aatttaaaat gggagttcaa agaaagaatt aaagaagagt gggagcagtt
      601 tttctttaaa aataaaatca gtaatattac aaaaaatgat tttttaaaac tactaagttt
      661 gagtagaaat gtagtggaaa tagaaagtaa ttatattaat aatgatattt cgctaacttt
      721 taaacaaatg gctgatatgg caactcgaat aaagaaaaat atttactacg tagggaatca
      781 tgaagcattt gttcaagcta agggaagtgc tcattttgag ttttcaaaaa ggattaaaca
      841 aaattatagt tatagatgtg caattaccgg gattaaaact aaagactttt tagtcgttac
      901 acatattatt ccttggcatg aaaatgagtt tattagacta gatccttcaa atggtatttg
      961 tttgtcttta tttttagcaa aagcatttaa gaaaggcttt atcacttttt ctaacagtta
     1021 tagagttgtt ttatctaaag aagcagagaa agatgctgct ttatatgaag aattaaagat
     1081 ttatgaaaat caaaaaatcg aactaccaga ttgtcagaaa cctaatttga aatatttaga
     1141 ttggcataga gaacatattt ttaaaaatta attttgggag aggttgtatg acagatatta
     1201 gtcaattagt agaggtgcca tacatctttc attagtagat tgaaaaagag cacttgaaac
     1261 agataatttg tattatttag cctatggatt aacaacttta gataattgtt ttaaatatta
     1321 ccaattaatt acacaaaatg aacatttttg ctattctcaa caagaagaaa gagaaagtta
     1381 tcagcaaaaa tttcaaagta tgattaatgg tttaaaaatt aaaattggca tacaaagttc
     1441 aaatgatgat gcgcaataag ctacaaagtt tgatttttta attgaacgtt atatgttttt
     1501 aaaagaagaa agagcttcga attaatatct taatggagga agattagtaa atggctagaa
     1561 cgtataatgt gattaaatat gtcattaaga aaaataagga tgatcttaaa atacatggat
     1621 taagattata tgaatttaat ataaaagatg aaaaagctat aaatattagc caacccattt
     1681 ttgtgaacag agcaacattg attgagaaaa tacaaaatgg tgaagcattt acgcacgcct
     1741 ttagagtaaa tcacaatact tattatgcag gccaattaat ttctttatca ttgaataaaa
     1801 atggttatat taataacaac tttgaggcct cacgagatat tattagaaac attgaagtaa
     1861 gtattttaga ctatgaataa aggtaatact gatagaatac attacaatta aaaaagggag
     1921 tataggactc ccttaataag ttgatatata agtgacttat ttttttaata gaattttaat
     1981 tcctctgtta atgttcaaga tatataatat taaagcaatt aaaaagaata taatagtaat
     2041 aattaaaaga atgctaaata ttaataattg atgatccaaa ggtttatcaa agacttcttt
     2101 agaaaaaata aatgccaatc tacctataaa aaaactaacc caagtcataa tatgattaat
     2161 gaatgctttt ttgccatgtt ctgatgttgg ttcatcagca agtatccata caaataaagg
     2221 taaaatgaca ggtgcgaaaa agatacttaa ataactcaat gctgataaaa cgtaactatt
     2281 gtcattttta tgcatattac tatttgtatt ttccactttt ttcaccttct ttacttttct
     2341 ttactattat aaaaacatta atagcgagct attacaacag ttataagaga agcttacgat
     2401 attgtaagta tgtgttgtta taatcaatat aaaattaaga aagacctgag aatttatatt
     2461 atcatccaca atcgaagtga gtagcgaaat ttattggaaa aggtacttat attgaagata
     2521 ttgttaatgg ttcattttta aaaagccaag acgatttgaa gttaaaagct tctgacttca
     2581 ctgtcgaaga taactataac tacggtgtac ttatatttcc agagaatctt tctcttacag
     2641 ataataataa gaaattggaa tctttaataa aaactatact atttacaggt aaacgatatc
     2701 aatatactta ttttaaaatt tgatcttaat agataaggat ataatgattt aaacaattta
     2761 agagatatta ttttcacaaa aaaagaatgc tttttgttag aggcactgaa cccttaaaat
     2821 gggaacttaa taaaaacact atgttctagg ctgctaattc tctgtatttt acaggggata
     2881 agtagcctag tttttgttga attcgattat tattatagtt tttaatgtat tttcgacaat
     2941 atctattaca atatgattag agttattaag ctgattattg atgtaaaaag tttcagactt
     3001 tagcgaggaa tggaaacttt ctatcggggc gttatcggca ggtgttccct ttcgggacat
     3061 acttctgata atgccttttt cttcgcataa ttgataataa gcataagatg tataaacgct
     3121 gccttgatca ctatgtaata tacacccctc aggtatatcg atttgattta atgtatcatt
     3181 aactaaacgt tggtcttgtt tatcatctat tttatacgcc acaatttctc cgttataaat
     3241 gtccattatc gaagataaat acaacataga atgaccaaat ggtaaataag taatatcggt
     3301 tgttaatact tccatgggac aactcgcttt aaattgtctt tgtaataaat tgtccgtttt
     3361 ataatacggt ttacctattc ttgtcgtctt tttaggtcta actcggcagt tcaaatgatg
     3421 cttctgcatc actctctgta ctctcttatg attaattggt gatgtataac attgattaat
     3481 caatgctgta atcttacgat aaccgtaggt atattgttta ccaacatgtt gtgaaaatct
     3541 ataactttcc ccatttcgat accatcgcca ccaatttttc acttgtgttc tatttctaat
     3601 atttaattct ttcataattt cttttgttgg aaatcctgct gctttcattt caactgcttt
     3661 atactttgtt tctactgaat aagaagctct tttcatagaa aaaaacacct ccgtatgatt
     3721 cattttaata tgaattcaac gaaagtgttt tt
//
LOCUS       DANJFZ010000173         3740 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.175, whole genome shotgun sequence.
ACCESSION   DANJFZ010000173 DANJFZ010000000
VERSION     DANJFZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.175"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            263..2413
                     /locus_tag="PWN45_002662"
     CDS             263..2413
                     /locus_tag="PWN45_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL0624365.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            2406..2861
                     /locus_tag="PWN45_002663"
     CDS             2406..2861
                     /locus_tag="PWN45_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL0624366.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            3508..3590
                     /locus_tag="PWN45_002664"
     tRNA            3508..3590
                     /locus_tag="PWN45_002664"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3541..3543,aa:Leu,seq:gag)
     gene            3613..3686
                     /locus_tag="PWN45_002665"
     tRNA            3613..3686
                     /locus_tag="PWN45_002665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3645..3647,aa:Gly,seq:tcc)
BASE COUNT         1421 a          484 c          711 g         1124 t
ORIGIN      
        1 taagtgaaga agatatctaa ttgtcgtttt aaataggtgg gttgctatta gaataaaaaa
       61 agtagtctta gattatgaaa tttagaaatg atggtgtgtc attttcaata atcttagtgc
      121 gttttaaaat atagtatgac ctaatcattc gttttaaatg ttttggaagt gaaaattaca
      181 ttaagtatca taccttaata gaagtatttt agaatatgtt aaaataaatg agtaaattta
      241 agaaaaagtg tgggttaagt aaatggacaa tcaattgatt aattcaatca tagagaaata
      301 tcaatttagt aaaaaacaaa ttgaagcagt attaacactg ctagaagaaa aaaatacagt
      361 accatttatt gcgaggtatc gaaaagagca aactggtgga ctagatgaag ttcaaataaa
      421 gcaaattgat gacgaatacc aatatatggt caatttacaa aaacgtaaag aagaagttat
      481 caaaaatata gaacagcaag gattacttac tgaggaatta aagaaggata ttttaaaaca
      541 gaacaaatta caacgtgttg aagacctata taggcctttt aaacaaaaga aaaagacaag
      601 ggcaactgag gcgaaacgta aagggttaga gccattagcg atatggatga aggcacgtaa
      661 acatgaagtc tcaattgaag aaaaagcaca acaatttata aatgaagaag tgcaatcggt
      721 tgaagatgct atcaaaggtg cacaagatat tattgcggaa caaatttcag ataatcctaa
      781 atatagaaca aaaattttaa aagatatgta tcatcaaggt gtgttaacta catctaaaaa
      841 gaaaaatgct gaagatgaaa aaggtatttt tgaaatgtac tatgcatata gtgagccaat
      901 taaacgcatt gctaatcata gagttttagc tgttaatcgt ggtgaaaaag agaaagtatt
      961 atctgtaaag tttgaattcg atacgacatc agtagaggat ttcattgcac gtcaagaaat
     1021 caatcataat aatgtaaatc gcagttatat tttagaggcg attaaagata gcttgaaacg
     1081 cttaattgtc ccttcgatag agcgtgaaat ccatgctgat ttaactgaaa aagctgaaaa
     1141 tcatgcaata gatgttttta gtgaaaactt aagaaatcta ttactgcaac ctccaatgaa
     1201 aggtaaacaa atattaggcg tagatccagc atttagaaca ggttgtaaat tagcagtcat
     1261 taacccattc ggtactttta tagcaaaagg tgtgatttat ccgcatccac cagtttctaa
     1321 aaaagaggca gcagagaagg attttgtaca aatggttaaa gcgtatgatg tgcaattaat
     1381 tgcaattggc aatggtactg caagtcgtga aacagaacaa tttgttgcag atttaattaa
     1441 aaagcatcag ttgccagtac aattcatcat tgtcaatgaa gcgggcgctt cagtatactc
     1501 agcatcagaa attgctagag atgaatttcc tgattttcaa gtggaagaaa gaagtgccgt
     1561 atcaattgga cgacgcgttc aagatccatt aagtgaatta gttaaaatcg atcctaaatc
     1621 tattggtgtc ggacaatacc agcatgatgt taatcaaaaa gcactagaaa atgcattaac
     1681 tttcgttgtt gaaacagcag ttaaccaagt aggtgttgat gtcaatacag catcatcatc
     1741 attattacaa tatgtgtctg gtctaagttc gcaaattgct aaaaatataa ttgcatatag
     1801 agaagaaaat ggcgcaataa agcataataa agagttaagc aagattaagc gtttgggtgc
     1861 taaaactttt gagcagagta taggattttt aagaatagta gacggttcag agccattgga
     1921 taatacatcg attcatcccg aaagctataa agtaacgtat cagttattag ataaactcgg
     1981 atttggtggc aatgatttag gtagtgatgc attaaaagct aaattaaatt cattggatat
     2041 ggatgaactg gcgattgaat tacaagtcgg tgtacctaca ttagaggata ttattaaatc
     2101 cttgaaagca cctaatcgag atccaaggga cgagtttgaa acaccaatcc ttaaatccga
     2161 tgtgctatca attgaagatt tacaagaagg aatgaaattg agtggtacag ttagaaatgt
     2221 agtagatttt ggagcatttg tagatatagg tgttaaacaa gatggacttg tccatgtttc
     2281 gaaactttct aagaagttcg taaaaaatcc aatggatatt gtcagtgttg gtgatatcgt
     2341 tgacgtttgg atttacagta ttgacaaaaa taaagacaaa gtatcattaa cgatgattga
     2401 tccacatgaa taatgacaaa cttcagcgaa tggttgagaa tctttcggaa gaaaaatttg
     2461 gacgtacgtt tcgacattgt gcatatttta ataaaaggtt acgtacaaca ggcggacgtt
     2521 atcttttaaa gtcccatgat attgaaatta atccaaaaca atatgagcat tatggagaag
     2581 atgctgttgt aaaaattata ctgcacgaat tgtgtcatta tcacttgcat attgctggta
     2641 aaggttatca acataaagat caagatttta aacgattgag ccaacaggtt ggagcgccta
     2701 gattttgtaa cagcattgaa agctatcaac aacgggcaaa ttatgaatat tattgcacta
     2761 aatgtcatgc aaaatatata cgtatccgta aagttgatac gaatcgtatg cgttgcggac
     2821 attgcaatgg aaaattaaga atgaaaagac aattgaaata agtacaatac aaatagagct
     2881 aattcgtgta tatgtaagat tatttatgag ttgtatggaa tttatataga atttatcagt
     2941 gagttatttt gattggtaaa catagtaatt gaataagaag aaattgtgtt tataaaatgt
     3001 ttaaagccga gattgtcgta atttaatact tcgcaatctc ggcttttcta cttatgatat
     3061 aggtaatggc ataaattgtg gcttgatagg atgaaattaa aatgattaga ttaaaaatct
     3121 tggcgtattt tagtaataat taatatataa actttaccca attaaagctt taaagtagca
     3181 caaaagataa ttatacattt ttcatgacat tttgattgaa cttttctatt gactgtacag
     3241 cttatagtta gtatgattaa acacgttcct taaaaaagaa caaattaaat aaactttgat
     3301 ttgaaagaca gtcaattcta aatttaattt ataaaatatg ttgacaataa gttctaaatg
     3361 ttttattata aatagcgttg tgttaacaac tgataacaca aattaattta atgtaaaaaa
     3421 acgattaaaa gttattgaca aatattttta aacataatat aataaataac gttctgaaaa
     3481 acgaagatgt tttaaatgcc tatatccgcg gtcgtggcgg aatggcagac gcgctaggtt
     3541 gagggcctag tgggagaaat cccgtggagg ttcaagtcct ctcggccgca tcaaaattct
     3601 taatttaaat aagcgggtgt agtttaatgg caaaacctca gccttccaag ctgatgttgt
     3661 gggttcgatt cccatcaccc gctccataga taattttaat gaacattgaa aactgaatga
     3721 caatatgtca acgttaattc
//
LOCUS       DANJFZ010000174         3722 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.176, whole genome shotgun sequence.
ACCESSION   DANJFZ010000174 DANJFZ010000000
VERSION     DANJFZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.176"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(482..3538)
                     /gene="fnbA"
                     /locus_tag="PWN45_002666"
     CDS             complement(482..3538)
                     /gene="fnbA"
                     /locus_tag="PWN45_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDL0624367.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(3522..3623)
                     /locus_tag="PWN45_002667"
     CDS             complement(3522..3623)
                     /locus_tag="PWN45_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624368.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
BASE COUNT          952 a          714 c          551 g         1505 t
ORIGIN      
        1 ataacaggct tgtatttttt atacttactt tttaaattaa attcaaattc atctaattta
       61 aaacaatata ctaaaccata cataataatc gcctgtacaa tgcatcatta acaagtcact
      121 gaaacgcctt tcattgtatt aataacgtca ctataatttt tatatcgttc ggtttttgtt
      181 tgattttaat gattatttat acaaaaacag ccgtatttca agccgacatt ttaaatttaa
      241 ctaaatttgc atctagttaa taattgcatt tatcaaattt gtcttattga tccaatctaa
      301 tttgtactca caaactagtt taaaattcta actttatctc tcagttcgtt atcaatcatc
      361 agacataaac caatgaagca atcagaaaac actctaattt tctattagaa atttgattta
      421 atataaaaaa acaggcttac ttcatataat ttatgaaata aacccgtcaa tttttgttta
      481 attatgcttt gtgattcttt ttatttctgc gtaataatgc taaacctaga atgctgaata
      541 atccgccgaa caacatacct ttgtttgttg attcttctcc acctgtttca ggtagttcag
      601 atttcttaga ttgtggtttt ttagttggtg ccactgcttt aaccttttca ttgatttcaa
      661 taacaggtgt tactacttta ccttgttcca ctggtttaga aggcttttta ggttcttctt
      721 tggcaggtgg tactggttta ccaggttcag ctggtacctc tggtgttggc ggtgttggag
      781 tttctggctc actcggcact tctggtgtcg gtggtgttgg tgtttccggc tcacttggta
      841 cttctggtgt tggtggcgtt ggtgtttccg gctcacttgg tacttctggt gtcggtggcg
      901 ttggtggcac gattggaggt gttgtatctt cttcaatcgt ttgttgacct tcattttggc
      961 cgcttacttt tggaagtgta tcttcttcaa agtcaacact attgtgtcca ccgaattgat
     1021 aacttggttt atctttattt gtatcttctt caataatttc agtgtgctta ttgaatccgt
     1081 gaatatgtgg cacactgtcg aagtcgatat caatgatgtt accgccatgt tcatacttag
     1141 gtttgtcttt ttctgtatct tcctcgaatg actgattacc tttattttga ccatgaattt
     1201 gaggtacact atcaaaatcg atatctacga tattgccacc ttgttcatat ttaggtttgt
     1261 cttcttctgt gtcttcctcg aatgactggt taccgctatt ttggccacct tcataaccta
     1321 attcactctt aatatcaacg tggctatttt cttcgatttc ttcaatcacg tcataattcc
     1381 cgtgaccatt ttcagttcct aaaccagaat gagaaatatg atgattgttt tcagtaattt
     1441 cctcgactgg tccttgtgct tgaccatgct cttcaggtaa ttcatccact aattcaatca
     1501 gattactttc agttgtatat tctttcgtat cttcaactgt tgtatgatcg ctcactgcgc
     1561 cagttacaat accttttgta gactcttcgt caaattcaac taagttagac tcagtagtaa
     1621 cctgaccacc acctgggttt gtatcttctt catattcaac aacatcagcg tgatgttttg
     1681 aattttcatg tgtagattct tcaaagtcaa ttggatttga ttcctcagag gactcagtgt
     1741 atcctccaac gtgacctgct tcgctatcca cagcagtatg gtaatcgata tcaatagctg
     1801 atgaatccgt ttcttctatt gtttcaatgt atccatcaac atatccacct ccaccatcta
     1861 tagctgtgtg gtaatcaatg tcaagagttg atgaatcata ttcctcttca acagtagtta
     1921 ctaaattctt atcatattga cctgtaagag tttctttaat tgtatcttct ttatattcaa
     1981 atttattatt ttgaataatc ggaccatttt tcccatttcc gttcgcttta ttactgtata
     2041 aaactaaacc attatcccaa gttaaggtat atcctctatc ataataatac ttataaagtt
     2101 gctctggatg tcctaccatt tgtgttctaa aatcaacttc atcagtacca tttaaatact
     2161 ctccatcata gtgaacaaca taagttttat ctagattttc tatattcaat gaatagcttc
     2221 cattattttg taaattcaaa ttcccactca tattacttgt gacttcttta aatttagaag
     2281 tatctgtcgt atttgcatat acactcttcg ctatgtcttc attattaccc aagtattcaa
     2341 atatcctaac ttttggttga tttccattct gattactacc tttcattaaa gttccagtaa
     2401 cagtcacact tgtcgtttta ccattattag gtttaataaa tgcaacatgc gaaaatctat
     2461 tattcgcttt attaaatgtc tcaatcgatc catttaaatt ggcataataa ttcccaatac
     2521 catctttata tttaacatct aattcctttg aagtttgttc ttcatttagt gttgaagtta
     2581 tagtttgatt tccattagtt tgtacagttt taggatcaat aaataaatta atttctagtt
     2641 cagccgttac atcaacctta tcttcaatat catttgtaaa tgtatatcta atctttccac
     2701 cttctaaaac ttcacctgtc gccattacga ctgaaccatt tttaatttct ggtacttttc
     2761 tagcagttga tacgccatgc gtatttacat tatttgataa agtaaagtca aagtagtcac
     2821 cttgatgtaa accattctca aatttcaact tatattttag taccgctcgt tgtcctgcat
     2881 gaggttctac tttatttgta ttgttatgcc cctcaataga accaatttct actgtaactt
     2941 tacttgttac atctgtaccc gtttccactt tcgcgttact agcttcctta gcttccgcta
     3001 catctgctga tcttgtcaca cgtggcttac tttctgatgc cgttcttggc tgtgccactt
     3061 caacttgtgt ttctgcgact tgattttgtg tagccttttt aggtgttaaa tctacttgtc
     3121 tttgatctcc gctattgtct tgagattgtg ttgtttcctt aacttgaggt ttcgcttctt
     3181 ccttaactac ctcttcttta actgtttcta tatttgctgg ttgtgcagtt tgtggtgctt
     3241 gtactgcttt tggtgcttct tcagttgtta cttgtgttgc gtttgacggt tgttctgtta
     3301 ctgttgcgtt atatgattga gtttcttcta tatgattaac gttagttgca gttgtttgtg
     3361 tttcacttgt tttattatca gtagctgaat tcccattttc ttctactgta gttgtctttt
     3421 gttctgatgc tgcagcttct ttgtcttgtc ccatcccaac aacgatcatt gttcctaaga
     3481 atactgatgc tgctcccaat ttatgttttc taatgccgta cctaagattg tttttcacta
     3541 taatatctcc ctttaaatgc aaaattcatt aattttttaa acttaataaa tgcaagtcta
     3601 tattgttcgg tttttaaaag caatacaaaa aagcgagtta atgaaaagtt aagattgtca
     3661 ttaattttat gttaattgat aattttatta tttgaaatat acctataaat tgtattcaag
     3721 tc
//
LOCUS       DANJFZ010000175         3611 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.178, whole genome shotgun sequence.
ACCESSION   DANJFZ010000175 DANJFZ010000000
VERSION     DANJFZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.178"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            277..2232
                     /locus_tag="PWN45_002668"
     CDS             277..2232
                     /locus_tag="PWN45_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624369.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            2258..3001
                     /locus_tag="PWN45_002669"
     CDS             2258..3001
                     /locus_tag="PWN45_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDL0624370.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            3065..3391
                     /locus_tag="PWN45_002670"
     CDS             3065..3391
                     /locus_tag="PWN45_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDL0624371.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
BASE COUNT         1394 a          399 c          594 g         1224 t
ORIGIN      
        1 cagaaaatgc ataaaaattc gacacgaacg atcaagtttc tatgattatg aaagctttgg
       61 tatttggatg aaaatgtata tttaatggat aaaatatcct aatttagcat aaaaaaatgt
      121 tttaataaaa gtattatttg atataatcga tttatgtttt gttactgcta aaaaacatgt
      181 ggcgatataa ttggtagtga atattagatg ttgacaattg atttgagaaa gacaaacagg
      241 agtaaatcaa tatatttgta tatgtgagga tgtaagatga cagataatta ttcactacat
      301 atttataaaa gtatgaaaat gagtagtgtg aaggaagcga acttaaaaat tatatattgg
      361 ttacaaggta caggtgtagt ttcaatcaat ctacaacaat atgatctaaa aagaaattat
      421 gtcaccatta ttatgttgaa tgatttgtat caaattgatg gtaatgatga tagtgtatgt
      481 tgtgttgttg aagtaagtgc aaagacattt ttgaagttta tgaatgcaaa ctatatgatg
      541 cgtggcaaga tattaacaaa tgaggttgct agcactttta gaatcttgat taagtattta
      601 atctacagca aaattaaaca acagtcttat aatgctaatg ataagattat taattacatg
      661 tgtgttgaat taatgtcatt aaatacatac gatggtagtc gtcatttagt agccgaagaa
      721 gtgcacgatt atttaacaaa caaccaccat aaaaagataa ataggaaaga tgtcattaat
      781 caagttagta ttacaaacaa agctttaagt gacatgttta aagcgacacc ttatagtaat
      841 tttattcaat atttaaatca tattagactg gagcattgct taattgatat tttaacttct
      901 aagaagccta ttgaagaaat cgcaagtagt catggtttta atcattattc tcgttttata
      961 cacctcttta aagaaacgta tggtaacact cctaaattaa ttagaaaaag atatagtcca
     1021 acatctcatt caattaacca ttcccaatta gttgaaattg ataaaaatat aattgaatta
     1081 tttgatgata ctaatcaaag ttcttcaagt atgcgcgaga ttgatatacc ttttgagcct
     1141 acaaaaagga gtcaaatgta tcaacctaaa aacatttata ttaaaggaag taatttttat
     1201 tggatggatt attatactat taaacaaatc attcaaagat taggtgttaa tcctgaaaac
     1261 ttgcatataa ttgttacaat cgacacaagc aatgagctat cgattgaaca aatcacttta
     1321 ttacttcaaa agatagtgaa ttacaatgta aatgtagtgt ttcgtattaa acatgaatat
     1381 aaggaattgt taacgagtgc tgaaagaggc aagatagaaa agctagtagc tacaattttt
     1441 aatatgactt ttgagagtaa tagatgtaag attgcatttt taattaacga cttaaatact
     1501 actgcaattc aaaaactgaa acgtttaata gataattata taggagtatt tgaattaata
     1561 tatcatctgg aaccaaaaga gttgaatacg cgctatgata aagaaattga tcatctaata
     1621 gactatttta tcttaccgct aagacaaata gaacaggtgt ctattgcacg ttctaaaatt
     1681 atatgtgatg tagatttgat taatgagaat aatgacttaa aagaaaaaat cacaatgtct
     1741 gatattaata aggtttctga acttttgaca aattatacaa aatttcgagg acttattatg
     1801 cacatgtcaa ttataagtga atcgagcaaa gaaataaatt taactagatt atcaatattc
     1861 atttttatca taaatatact taatcaatta cgagggattg ttatttatca aaatgacacg
     1921 atgattgtaa cgaagttcaa acatgaagta caatgtgtca tttgtttgcc agccgcattg
     1981 ttaaatgaga attcagacgc tatacgttta tcgtgtaagg gtcttcaaca atttagccat
     2041 gctaaaacta aacaaattgt tttaagtata gataaagata atcatacttc cgaaaatggt
     2101 gtacttgtga atgatattat gcaacaacac agttattgga aagctaattt tatagaaaga
     2161 aatatttatg atcattcatt aatggtctca agcaatagta ttgttcattt gaaatatcat
     2221 cttgagatat aggcatggtt taagagaggt tttaaagatg tctaaattta aagttaagcg
     2281 tttaactgca cacatactaa tgttgaaagt cattaatgat atgttgaaat attcatttca
     2341 tctcactttg actcaagtga aactattaaa tctattagtt aaatatgata attctgattc
     2401 caatagcgct tcaatagata gtttattaaa aatgaaagaa atccaatcta agatggccct
     2461 tcttcaaatg ttaagttatt taaacaatca tcaatggcta ttgaaaggaa gagatcaaag
     2521 ggatcaacgt aaattgacta tttcgattaa taaaatgcat atagataaaa tagaatatat
     2581 gaataatgag cttagtgact atattgaacg ttattttggt caatatacat ttgattttag
     2641 ttgtgactat gtatcgtatt tattgtcgtc acaagaatta ttgcggaatc taaagtgtta
     2701 tttaaatatg tgtcaattat cattagaaga gttatacgtt ttaggaatat taaatctgca
     2761 taaaggtcaa ttgacggtta aagaattaca gggtgaattc catcacccga tatttgcggt
     2821 tagcccgatt ttaaagtgtt taattttgaa gggattagtt aaaaaagaga gatgtgaatt
     2881 agatgaaagg cgtgttattg taacaatcaa gcgagaaaag ttttctaaag tcactatgct
     2941 tattcaagca tgttataatt atcttgaaaa gggaatacaa gttaatctta ataataaata
     3001 gttaatcact tgtatttcat atacctatga agaatagttt atagttttaa gtgggaggga
     3061 tattatgatt ttagataatg tgaatccaaa tgatttattt ccaactgaaa aaaagggtcc
     3121 gtcagtgcta ggaataattg agtatcaagt tcaaggtgag aatgaatttg aaggggcatt
     3181 tatagcaaca aatgaaagat taattatgaa tgtagatatg aatggccaat tttattatag
     3241 aagtattagt tataatgaaa tagagaagat agattatgat ggtcaaacta taatgttcaa
     3301 atttaatatt ggtaatgtcc caatgcatga tattaaaagt gctaacgttg aaatgtttgt
     3361 tgaatatgta aaacaacata tgatagtata atgtcaatgt atgtaggttg ataagaagag
     3421 tttgaatgat aactcattgc atttaaagat aaatggcatg cgttatgatt taaagctctt
     3481 ctttttttat attttataaa ttttgtgagc aagccatcca attaatataa aaaatatcaa
     3541 atcaaacacg ttgatataga ttaaaatttt aaaagtttac atatcagtta agatacaaaa
     3601 tattcagact a
//
LOCUS       DANJFZ010000176         3566 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.180, whole genome shotgun sequence.
ACCESSION   DANJFZ010000176 DANJFZ010000000
VERSION     DANJFZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.180"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            206..1693
                     /locus_tag="PWN45_002671"
     CDS             206..1693
                     /locus_tag="PWN45_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDL0624372.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            2339..3298
                     /locus_tag="PWN45_002672"
     CDS             2339..3298
                     /locus_tag="PWN45_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0624373.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(3360..3563)
                     /locus_tag="PWN45_002673"
     CDS             complement(3360..3563)
                     /locus_tag="PWN45_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624374.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
BASE COUNT         1230 a          500 c          669 g         1167 t
ORIGIN      
        1 ttgaaaagtg caatatgttt tcgaaaataa gattattttt atgtgcaaaa acgacgcaaa
       61 agttttaaaa atgagacttc tgtgagctga ttattttata aaatgtaaac gcttactata
      121 taatgtgaat catatcgttt aaaagcatta ttaaatatga tgctaagaga tttatattat
      181 agccaataaa caaaggagag ataatatggc agtaaacgtt cgagattata ttgcagagaa
      241 ttatggttta tttatcaatg gggaatttgt taaaggtagc agtgacgaaa caatcgaagt
      301 gactaatcca gcaactggag aaacactatc acatattaca agagcaaaag ataaagatgt
      361 cgatcatgca gtcaaagtgg cgcaagaggc atttgaatca tggtcattaa cttctaaatc
      421 agaacgtgca caaatgttgc gtgatattgg tgataaatta atggcacaaa aagataaaat
      481 tgcaatgatt gaaacattaa ataatggtaa accgattcgt gagacaacag caattgatat
      541 tccatttgct gcaagacatt tccattattt cgcaagtgtt attgaaacag aagaaggtac
      601 agtgaatgat atcgataaag acacaatgag tatcgtacga catgagccga ttggcgtcgt
      661 aggtgctgtt gttgcttgga acttcccaat gctattagct gcatggaaga ttgcgccagc
      721 cattgctgca ggtaatacaa ttgtgattca accttcgtct tcaacaccat taagtttatt
      781 ggaagttgct aaaattttcc aagaggtatt acctaaaggt gttgtcaata tactaacggg
      841 taaaggttca gaatcaggta atgcaatttt caatcatgat ggtgtagata aattatcatt
      901 tacgggctca actgatgtag gttatcaagt tgccgaagct gcagcaaaac atctagtacc
      961 cgctacatta gagcttggtg gtaaaagcgc caatatcata ttagatgatg ctaatttaga
     1021 ccttgcagtt gaaggtattc agttaggtat tttattcaac caaggtgaag tatgtagtgc
     1081 aggttctcga ttattagttc atgaaaaaat ttatgatcaa ttggtgccac gtttacaaga
     1141 ggcattttca aatattaaag ttggaaatcc acaagatgaa gctacacaaa tgggtagtca
     1201 aactggtaag gatcaattag ataaaattca atcatatatt gatgcagcaa aagaatcaga
     1261 tgcacaaatt ttagcaggcg gtcatcgctt aactgaaaat ggattagata aagggttctt
     1321 ctttgagccg acattaattg ctgtgccaga caatcatcac aaattagcac aagaagaaat
     1381 atttggacca gtgttaacag tgattaaagt gaaggacgat caagaagcaa ttgatatagc
     1441 taatgattct gagtatggtt tagcaggcgg tgtattttct caaaatatca cacgtgcatt
     1501 aaatattgct aaagctgtac gtacaggacg tatttggatt aacacttaca accaagtacc
     1561 agaaggcgca ccatttggtg gttataaaaa atcaggtatc ggtcgagaaa cttataaagg
     1621 tgcgttaagt aactatcaac aagttaaaaa tatttatatt gatacaagca atgctttaaa
     1681 aggtttgtac tagaataaat atcgtttctg aagcgtgttt gtaggtcagt ctagcggtaa
     1741 gtcttaacat ttaacggcgt tgtttagatt ttaagcaaaa caaaatatat aggaacacgt
     1801 atcatgatat taggatataa tgactaaaat aatagcagta ggatggtttt taattgcaaa
     1861 tcatcttact gctgttttta attatgctaa tttgcgatgc ggctattata aggacagagt
     1921 tgtttattaa ttatggtgat ttagaaatat gaagttcaat atgcaaagtc atcgtttgtt
     1981 ttaatatgcg gaacaatcat taaagttatt gcgatttttt gaacttaatg aaactaaaca
     2041 ataaatttga gatacttttt tgtcattttt atgtaactaa cacaataatc tcgtacatta
     2101 ttaaaatttt ctatatgata ggaataaagc aaagcgcgag tgtgctgtaa aagttttcca
     2161 aggtgatatt acataaagct ataaagggta aagattaatg agttgtcatg taaatgacga
     2221 tgatgtataa atcatggtta attacggaag cattaatatt aacctgagaa gctataaaga
     2281 attattttta aaagcgacaa tattaaatac gacgcattta tttaggagtg gcaaacgtat
     2341 gaatgggaaa aaggcgaata cgataaacag atacaaatat tttcatcatg tcaatcatca
     2401 aaaaattcaa caaagttcta aaaagacgct gtgggcatca ctaatcatca cattgttatt
     2461 tacagtgatt gaatttgtcg gaggtttagt atctaattca ttggcattac tgtcagattc
     2521 atttcatatg cttagtgatg tattagcact tggtttatct atgttggcca tttattttgc
     2581 aagtaaaaag ccgactgcac gatacacatt tggatattta agatttgaga tattagctgc
     2641 atttttaaat ggtttagcat taattgtaat ttcaatctgg attttatatg aagctattgt
     2701 acgtattatt tatccgcaac caattgaaag tggcattatg tttatgattg ctagtattgg
     2761 tttactcgtc aatattattt tgactgttat ccttgtaagg tctttaaaac aagaagacaa
     2821 tatcaatatt caaagtgcat tatggcattt catgggagac ttattgaact ctattggtgt
     2881 catcgttgca gttgtattga tttactttac aggatggcgc atcatcgacc caatcattag
     2941 tattgtaatt tcactcatca ttttacgtgg tggttataaa attacgcgta atgcgtggtt
     3001 aattttaatg gaaagtgtgc ctcaacattt ggatactgat caaattatgg cagatattaa
     3061 aaacatagat ggcatattag atgtacatga atttcatttg tggagtatta caacagagca
     3121 ttattcatta agtgcccatg ttgtgttaga taaaaaatat gagggtgatg attatcaagc
     3181 gattgatcaa gtatcatcat tgttgaaaga aaaatatggc attgcacatt caacgttgca
     3241 aattgaaaac ttgcaattga atccattaga tgagccatac ttcgacaaat taacataaat
     3301 aaaacattgt agcgcctaaa acattaatct atgtcatagg cgcacgtttc gttttatact
     3361 tatgttgcat catttaaatg attttcgtca atttctttga tgctatctac atctaacacg
     3421 acatctttag gtttcaaaat atgaatatgt ttttcatcat ttgtatgtaa aatgcgttct
     3481 atgatgtacc tttgaccggc cattgtttct acagcaatct ttttgtttct agctaaactt
     3541 gctacgacag attctttatc cataat
//
LOCUS       DANJFZ010000177         3496 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.181, whole genome shotgun sequence.
ACCESSION   DANJFZ010000177 DANJFZ010000000
VERSION     DANJFZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.181"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            68..3097
                     /locus_tag="PWN45_002674"
     CDS             68..3097
                     /locus_tag="PWN45_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL0624375.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(3168..>3496)
                     /gene="mscL"
                     /locus_tag="PWN45_002675"
     CDS             complement(3168..>3496)
                     /gene="mscL"
                     /locus_tag="PWN45_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL0624376.1"
                     /translation="NVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEW
                     SFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRD
                     LLREKK"
BASE COUNT         1475 a          421 c          578 g         1022 t
ORIGIN      
        1 atcggatatc caatctaaaa agataaaaaa tattttagaa aacgaattga gaaaggagga
       61 ttaatgaatg aaaccattac atttaaagtt gaataatttc ggcccctttt taaaagaaga
      121 aattgatttt tctaaaattg ataataatga attgttttta ataagtggta agactggatc
      181 gggtaaaaca atgatttttg atgcaatgac ttatgccttg tttggtaaag catcaactga
      241 acaaagagaa gaaaatgatt tgagaagtca tttcgctgat ggtaaacagc cgatgtcagt
      301 aacatttgaa tttcaattaa atcatcgaat ttataaagtg catagacaag gcccttatat
      361 caaagaaggt aatacaacaa aaacgaacgc taaatttgat gtatttgaga tggtggatgg
      421 caagtatgaa attagagaaa gtaaagtaat ttcaggtacc caattcatta ttgaattatt
      481 aggagtaaat gcagatcaat tccgacaatt gtttattttg cctcaaggtg aattcaaacg
      541 ctttttaata tcaaacagtc gtgaaaagca agggatatta agaacactgt ttgacagtga
      601 aaaatttgaa gctatacgag aaatattaaa agaagaagta aaaaaagaaa aagctcaaat
      661 cgagaataga tatcaacaaa ttgacctttt atggcaagaa attgaatcat ttgatgatga
      721 caatataaaa ggcttattag aggttgccac tcaacagata gacaaattga ttgaaaatat
      781 accactttta caagctaggt cgaaagaaat actagcatct gtaaatgaaa gtaaagaaac
      841 tgctattaaa gaatttgaaa taatagaaaa gaaaacatta gaaaataata tattaaaaga
      901 taatattaat caactcaaca aaaataaaat tgatttcgtt caattgaaag aacaacaacc
      961 tgaaatagag ggaattgaag ctaagttaaa gttgttacaa gatattacaa acctattgaa
     1021 ttatattgaa aatagagaaa aaattgaaac taaaattgct aatagcaaaa aagatatttc
     1081 taaaaccaat aataaaatat tgaatcttga ttgtgataag cgaaacatag acaaagagaa
     1141 aaaaatgtta gaagaaaatg gagatttaat tgaaagtaaa atctctttta ttgataaaac
     1201 tagagtatta tttaacgata ttaataagta tcaacaaagt tatctcaata ttgaacgctt
     1261 gagaactgag ggtgaacaat taggtgatga attaaatgat ctaattaaag gtttagaaac
     1321 ggtcgaagat tcaataggta ataaccaaag tgattacgag aaaattatcg aactaaataa
     1381 tacgataacg aacataaata atgaaattaa tataattaaa gaaaatgaaa aagctaaagc
     1441 tgaattagat aaactattag gtagtaagca agagttagag aatcaaatta atgaagaaac
     1501 atctatattg aagaatctcg aaataaaatt agatcgctac gataaaacaa aattggactt
     1561 aaatgataaa gaaagcttta taagtgaaat taaatctgct gtaaatattg gagatcaatg
     1621 tccgatatgt ggtaatgaaa ttcaggattt agggcatcat attgattttg acagtattgc
     1681 taaacgtcaa aatgaaatta aagaaattga agcaaatatc cacgctataa aatcgaatat
     1741 tgctgtgcat aattctgaaa ttaaatttgt taatgaaaaa atatcgaata ttaatattaa
     1801 aacgcaaagt gatttttcac ttgaagtatt gaataagcgt ctgctagaaa atgaaaatgc
     1861 attgaataat caaagagatc ttaataaatt tatagaacaa atgaaagaag aaaaagataa
     1921 tctaacgttg caaattcata ataaacaatt gcgtctaaat aaaaatgaat ctgagttgaa
     1981 attatgtcga gatctcatca ctgaatttga aacactctcg aaatataata atatcactaa
     2041 ttttgaggtg gattataaga agtatgttca agatgtgaat cagcatcaag aactctcaaa
     2101 ggagattgaa gataagttaa tgcaattgtc tcaaagaaag ttaattgagc aaaataatct
     2161 aaatcattat gaaaatcaac tagaaactta caataatgac ttagaattga atgaacagtc
     2221 tattgaaatg gaaatgtcga ggctgaattt aactgatgac aatgatatag atgaaataat
     2281 agcctggagg ggcgagcaag aggaattaga gcagaaaagg gatacttata aaaaacgtta
     2341 tcatgaattt gaaatggaaa tagctaggtt agaatcatta actaaggata aagagttatt
     2401 ggactctgat aaattaaaag atgaatatga gctaaaaaaa ggaaagatga atacactgat
     2461 agatgaatac tctgctgttc attatcaatg tcaaaataat attaataaaa cacaatctat
     2521 agtttcgcat attaattact taaatcaaga attaaaggat caacaagaaa tatttcaatt
     2581 ggctgaaatt gtcagtggta agaataacaa aaatcttaca ttggaaaact ttgtcttaat
     2641 ttactattta gatcaaatta ttgctcaagc aaatctgaga ttagcaacaa tgtcagataa
     2701 tcgataccaa ctaattaggc gagaagcggt ttctcatggt cttagtggcc tagaaattga
     2761 tgtatttgat ttgcattcaa ataagtctag acatattagc tcgttatcag gtggagaaac
     2821 tttccaatcg tcgcttgcat tagctttagg gttaagcgaa attgtacagc agcaatcagg
     2881 aggtatttca ctagaatcaa tatttattga tgaaggattc ggtacattag atcaagaaac
     2941 gcttgaaaca gcgttagaca ctttattaaa tcttaaatca actggtagaa tggttgggat
     3001 tatttcacat gtgagcgaat tgaaaaatag aataccttta gttttagaag tgaaatcaga
     3061 tcaatatcag agttcaacaa gattcaaaag aaattaaaac ataaaaaata gagatggtac
     3121 tttggaatgt atagaacaac cttagcccat ctctttttat ttaaaaatta ttttttctca
     3181 cgtaataaat ctctgatttc agttaataac acaacatttt cttccacaac tgcttcttct
     3241 tcggcttctt ctttcttcat taatgtattt gcaatcttaa caaagatgaa taaagcaaac
     3301 gcgatgataa taaagtcgat aacagattgg ataaataaac cgtatttaat accccagaat
     3361 gaccattctt tagcaaaatc aactgatccg aaaattttac caattaatgg cataatgata
     3421 ttttctacta atgaagatat aatcttgttg aaagctgcac ccatcacaac agcaattgct
     3481 aaatctaaga cgttac
//
LOCUS       DANJFZ010000178         3403 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.182, whole genome shotgun sequence.
ACCESSION   DANJFZ010000178 DANJFZ010000000
VERSION     DANJFZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.182"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..334
                     /locus_tag="PWN45_002676"
     CDS             <1..334
                     /locus_tag="PWN45_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624377.1"
                     /translation="VIEEDNKIYYFDTSATFEDQEVKFEFALFDSDMDNLKHMDCDNP
                     VTEIYFIEPDLHFTIIDFNQELLCIYIDFDSGLRHSNMATESGISLRINVAKSDFTKF
                     INELASLH"
     gene            complement(824..1810)
                     /locus_tag="PWN45_002677"
     CDS             complement(824..1810)
                     /locus_tag="PWN45_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0624378.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            2338..3096
                     /gene="map"
                     /locus_tag="PWN45_002678"
     CDS             2338..3096
                     /gene="map"
                     /locus_tag="PWN45_002678"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL0624379.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
BASE COUNT         1195 a          521 c          613 g         1074 t
ORIGIN      
        1 agttatcgaa gaagataata aaatttatta tttcgacact agtgcaactt tcgaagatca
       61 agaagttaaa tttgaatttg cactctttga tagtgacatg gacaatttga aacatatgga
      121 ttgcgacaat cctgttactg aaatatattt tatagaacct gatttacatt ttacaattat
      181 tgattttaat caagaactgc tttgtattta tattgatttt gattctggtt taaggcattc
      241 aaacatggca acagaatctg gtatttcatt aaggataaat gttgctaaat cagattttac
      301 taaatttatt aatgaattag cctctttaca ttaatgattt aaatctgata tgtaattaca
      361 atcaaaaaag acagccacat ccctccgtag tttaggcgtg tggctatatt tgagtctgaa
      421 tatttatgct tgtaatttta aaaagggaca tgctatatac gataaaaaga ggcggggaca
      481 taaatcaatg ttctatgctc tacgaagtta tattggcagt agttgactga acgaaaatgc
      541 gcttgtaaca agcttttttc aattctagtc aggggcccca acaaagagaa attggattcc
      601 caatttctac agacaatgca agttggggtg tgggccccaa caaagagaaa ttggattccc
      661 aatttctaca gacaatgcaa gttggggtgt gggccccaac acagagaatt tcgaaaagaa
      721 attctacagg caaagcgagt tggggtggga cgacgaaata aattttatga aaatatcatt
      781 tctgtcccac tcccatggtg ccaattagca taaggtactt aaattaagca tatctgctgt
      841 ctagcagtcg ataaatcatt agaacttcgt atagtatatg acttttaatt tgattttcac
      901 cactaatttc aagtgctttt atagtcgaac gtaaagtttc tacagaatca tcttctctct
      961 taaaagaacc atcataaaat atatctttga tgctactact aatttttagc aatgccattt
     1021 tttcgtcacc tgaaaagtta acacgagtat ttttaggcaa gtaaatgata tttgataaat
     1081 gagtgataaa caaacgattc gtatatgcac gtttagttaa ttgattgagt aatttccaat
     1141 cacattcttt tttcttatga tagcttaatt catcacgttg ataacttatt aacgtttcaa
     1201 cttgattatt taaattgaaa atatttttat atgctttttc gcttttatca gattgcagtc
     1261 ttgataagat aagttcttgg cagcgattgt aaaataattt atacatcaag gcatctgtct
     1321 tacttaattt ttcttcgacc tgaccataat acttaggtgg aaacaccatg aagttaatta
     1381 aacctgatgt cacgagtcca ataattgctg tcaatgttcg agacaaaaag ttgaatatgt
     1441 aggcatcatg aatacctgga atcatagcta atgatgttag tacagcgaca ttcgtaccaa
     1501 cttgcaattt gagttttgta cagaataaaa tcgtgaacgt tgcactcaat gcatatgtaa
     1561 aaggtgattg atcgccgaat aaatatgtaa ataatactgc aaagcctgca ccaattaccg
     1621 tagcaggtaa tctacgataa cctttaataa gtgatgcctt ggcagttggt tcaattgtga
     1681 ctacagctgt taaaatggca tagatgggtg ttaaatctag tgccatacaa aagacagctg
     1741 ttaaaaaaat ggcaatacca gttttaattg ttctggcacc aattaaatgt ttataccatt
     1801 gatcgttcat tttttaacct ctaatcatcg taaaatctta gcgagcgctt tataataata
     1861 gtatcgtaca ttggaaaagt tcatgtatgt aaaatatttg aaataatcat acataagcat
     1921 tactttgatt ttcatataca ttaatcaaag ttctgggctc cacaatgtta tattgttagt
     1981 agttgactga aataaaatgc acttgtaaca agcatttttc aattatagtc cggggcccca
     2041 acatagagaa tttcaaaaaa gaaattctac aggcaatgca ggttggcggg gccccaacac
     2101 agaagctgac gaaaagtcag cttacgataa tgtgcaggtt ggcggggccc caacatagag
     2161 aaattggatc tacaatttct acaggcaatg caagttgggg tacaacgata aagaaatatt
     2221 ttttctttat cacactatgt ctcactcact ttccaaaata ctaaagtaac atctttagta
     2281 tatcaaagaa tttttgctat aataagttat aattatataa aaaaggaacg ggataaaatg
     2341 attgtaaaaa cagaagaaga attacaagcg ttaaaagaaa ttggatacat atgcgctaaa
     2401 gtgcgcaata caatgcaagc tgcaaccaaa ccaggtatca ctacgaaaga gcttgataat
     2461 attgcgaaag agttatttga agaatacggt gctatttctg cgccaattca tgatgaaaat
     2521 tttcctggtc aaacgtgtat tagtgtcaat gaagaggtgg cacatgggat tccaagtaag
     2581 cgtgtcattc gtgaaggaga tttagtaaat attgatgtat cggctttgaa gaatggctat
     2641 tatgcagata caggcatttc atttgtcgtt ggagaatcag atgatccaat gaaacaaaaa
     2701 gtatgtgacg tagcaacgat ggcatttgag aatgcaattg caaaagtaaa accgggtact
     2761 aagttaagta acattggtaa agcggtgcat aatacagcta gacaaaatga tttgaaagtc
     2821 attaaaaact taacaggtca tggtgttggt ttatcattac atgaagcacc agcacatgta
     2881 cttaattact ttgatccaaa agacaaaaca ttattaactg aaggtatggt attagctatt
     2941 gaaccgttta tctcatcaaa tgcatcattt gttacagaag gtaaaaatga atgggctttt
     3001 gaaacgagcg ataaaagttt tgttgctcaa attgagcata cggttatcgt gactaaggat
     3061 ggtccgattt taacgacaaa gattgaagaa gaatagttca acatatacta agactaaagt
     3121 atgaacatca tttagttccg gagcctattc atattggttt cggaactgtt ttataataat
     3181 taagaacaca atcaatgcgt catttcaaaa atatgttgta acaaagtagt ttttaagcaa
     3241 acatatcatc gacatcaacg aagatacata gcgcatttgg tattttaaaa cttattataa
     3301 aaggtgatag ttatgaacta tgttgaacgt tatattgaac agtttttgag agcaacagta
     3361 agaaataata tcaagcacta ccttttaatg ctagatgaaa aaa
//
LOCUS       DANJFZ010000179         3365 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.183, whole genome shotgun sequence.
ACCESSION   DANJFZ010000179 DANJFZ010000000
VERSION     DANJFZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.183"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(27..1345)
                     /gene="cstB"
                     /locus_tag="PWN45_002679"
                     /pseudo
     CDS             complement(27..1345)
                     /gene="cstB"
                     /locus_tag="PWN45_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            1401..1538
                     /locus_tag="PWN45_002680"
     CDS             1401..1538
                     /locus_tag="PWN45_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624380.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            1535..1906
                     /locus_tag="PWN45_002681"
     CDS             1535..1906
                     /locus_tag="PWN45_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0624381.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            2035..2655
                     /locus_tag="PWN45_002682"
     CDS             2035..2655
                     /locus_tag="PWN45_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624382.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(3175..3363)
                     /locus_tag="PWN45_002683"
                     /pseudo
     CDS             complement(3175..3363)
                     /locus_tag="PWN45_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1176 a          456 c          550 g         1183 t
ORIGIN      
        1 cttcttcatt agtataaata taattattaa gaaaggtata tatctttgta accttcgttt
       61 acattaataa tgttgtgata acctttgtgt tctaaaatac caatagctat agaacttcta
      121 atgccagact gacagtctac ataaataaca tcgtttctat tgaaaggtaa atctgtttct
      181 aaaagtttgc cgtgtggcac atgaaccgct tgggataagt ggccattatt ccattcatta
      241 tcattacgta catctaagac atgtgcttca ttaccagtta tgtctttact atgaacagat
      301 tgtgtttgaa tttgagcttg tggtaactga tatccagaca cattatcata tccaataagt
      361 tgtaaagtat gttatgtgtt gcttttgaaa caaggtgata gtctccaatc aagttaattt
      421 cttgattata gtttagatac cagccaattt gattgatgaa atttttatca tatggaatat
      481 tgattgtacc ttcaatatgt ccaccatgat aagcctcctt actgcggaga tcaaaagtta
      541 atctgtttgt acttgtagct ggataaaccg tataaggttg atataaattc ataccgaatt
      601 gattaatttt tttcatttgt gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt
      661 tatcgataaa ggtagcttca ttattttcag aaaaagccca gttcgtttgt ttttcatagc
      721 caagcgtaga tgttggaata gcacctaaag atttaccaca aggactacca gcgccatgac
      781 caggccaaat ttgaatgtag tctggcaagt ctttaatact ttcaatagat ttaaacattt
      841 gttttgcgcc tatttcagat aatccttcta ctttaacagc tttttctagt aaatcaggtc
      901 taccgatatc tcctacaaaa ataaaatcac cactgaatag tcccattgga acttgtgctc
      961 cagcaccttc gtcagtaagt aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa
     1021 gcacttttaa ttttatattt cctacataaa tatcatcatt atgttgaaca aaatgagtgt
     1081 ggttaggcat atttttataa cctaacatgt catcactttc gcccgataca taaatactag
     1141 catttaactt tatagcaaca tctctaattc ctgaaacaaa atttgcatgt atatgtgttt
     1201 cagctgcatg agtaatggtt aaactctctt catcggcaac tcgaatatat gaagataagt
     1261 cacaaatagg atcaatgatc atggcttctc cagttttttg acaaccgatt aaataagatg
     1321 cttgagataa atgtttatca taaattgatg atagtcaatt tgttatcaag ggtgataatt
     1381 atattaacta tggtaattac atggaattaa agttattaaa caaacttgat gatgaccaat
     1441 tagatttaga atttttacaa gataatgggt taaaaggtaa tgttcaaggt cctatgactt
     1501 taaaggaacc tttaaaaagt tatatacaaa agtcttgaaa acgaatattt taaatttaga
     1561 taatgagtta gaaaacactg aatatgtcga aggtaaacca tatgtacagt acggtattaa
     1621 atatgaaaga aatcaggcat taagaaatga agctattaaa attcatggaa ctacatgtaa
     1681 agtatgtgga tttgatttta aagctaagta tggcgattta ggtgagggtt ttattgaaat
     1741 tcatcattta aaaccaatgt tttcaataaa aagagaaata aaagtaaatc cacaaaaaga
     1801 tttagtccca ctatgttcta attgccataa aatgattcat agaaatacta aaaaaccttt
     1861 aacgattaaa gaattaacca aaatagttaa ttataatagc aaataattta atattttata
     1921 aactatcatt caaccctctt aatttattag gaggtttttt gtatttatgc tttcaaatgt
     1981 gtgatatact ttgtttgttg aaatatagag tatctataga tagggtgatt gagtatgaaa
     2041 ttcactgaag tggaagttat cgaacatctt gtaaaggcat ataaagaagc aggaaagcct
     2101 acttatcctc atgaaaattt atatcgagga cgtaatcata gtatttcagg tattggagaa
     2161 gacttgctgg gtgcttattt gattagtaga ttggaaggtg tccaaatatt tattgatcag
     2221 cctttatcta tgattgataa atctttaagt acaagatatc cggatttatt aatttgtgaa
     2281 gataatgaaa ttaaaaatat actagaagtt aaaatggact taggatatca aagaaaagat
     2341 tttatagatt attgccgaaa gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt
     2401 gtattgtcta gaaagagaga agacaaaatt cctatgaata tagctgatga tattaaattt
     2461 catgttgtga tttacagtga aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct
     2521 atcgttaatg aaacatgtcc acatattgaa gtatatgtcc taacaagcgg tcaacaccct
     2581 aatttagtaa atgttaatct tgaaggtatt aatattaata aagatgaatt tgaaatatta
     2641 gtaaatgcgt tataaaaaaa tagagcatcc tccacgttat ggaggtgctc tgttttttat
     2701 tgaaaagtat caagttaatt aatttaatat gcttaataag ttctaccttg acctttttct
     2761 ctagcttctg ttcgatctct tatgtactca gtacatactg gattttctgt taattcatca
     2821 actgtattag tttaatcaga agacgtgtct actttgtaag cttctaagaa cttattatat
     2881 gatatagcgt ttgagttttg ttgttcttct ataaatttct gtagttattt ttcaaaaacc
     2941 gcatcattaa ctgataagca gaagcgtatc acaaataaaa ctaaaaaata gattgtgtat
     3001 aatataaaag gaagggattt atattaaaat tttgaattca aaaattattg aaagggaagc
     3061 taccttagaa attgaatcta tggccactaa ctgctttttt atatcaccac ttattaaaaa
     3121 atgtaagaat attcctcagt gctaagtgaa ggaggttcta gttttattta atattcatat
     3181 tgataggtaa tggttaataa ttgcctgtaa attattgtta aatatcgttg ttatagtatc
     3241 atcattcata aaatttttat agattttatc aagtatttcc tcatcttcaa tagctgtaaa
     3301 gcgatgtact agtcttttta tgagctatat ataattttgc gaagctaaac tgtttgtaat
     3361 tattc
//
LOCUS       DANJFZ010000180         3259 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.185, whole genome shotgun sequence.
ACCESSION   DANJFZ010000180 DANJFZ010000000
VERSION     DANJFZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.185"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            140..775
                     /locus_tag="PWN45_002684"
                     /pseudo
     CDS             140..775
                     /locus_tag="PWN45_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="PWN45_002685"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="PWN45_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL0624383.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="PWN45_002686"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="PWN45_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0624384.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="PWN45_002687"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="PWN45_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1090 a          515 c          742 g          912 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcgcttaact tgctagcatg atgctaattt cgtggcatgg
     3241 cgaaaatccg tagatctga
//
LOCUS       DANJFZ010000181         3182 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.186, whole genome shotgun sequence.
ACCESSION   DANJFZ010000181 DANJFZ010000000
VERSION     DANJFZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.186"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            86..394
                     /locus_tag="PWN45_002688"
     CDS             86..394
                     /locus_tag="PWN45_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624385.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(502..735)
                     /locus_tag="PWN45_002689"
     CDS             complement(502..735)
                     /locus_tag="PWN45_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624386.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(716..1909)
                     /locus_tag="PWN45_002690"
     CDS             complement(716..1909)
                     /locus_tag="PWN45_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0624387.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            complement(2293..>3182)
                     /gene="sasG"
                     /locus_tag="PWN45_002691"
     CDS             complement(2293..>3182)
                     /gene="sasG"
                     /locus_tag="PWN45_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HDL0624388.1"
                     /translation="KERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKG
                     ESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIE
                     EPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPIT
                     VNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNP
                     NNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLI
                     FSSIIGIAGLMLLARRRKN"
BASE COUNT         1028 a          556 c          425 g         1173 t
ORIGIN      
        1 tactgttttt tagaaacata catatataag aaacgcatcc attactaaaa gacagtacat
       61 tatattctat caggaaggtg caacaatgac cgtcaacatc gtatttagta tcgtattctg
      121 cataagtatg gttatattag gcatttatgt cgcaataact aaagatttca cactaatctc
      181 ttttataaat caaacggcca ttgcagataa acacaagaac caaattgcat atatatttac
      241 gctatgtata agtttgagtg cagtattttt aatgtcttca attctaagct ttgaatatga
      301 ttttattgca ttagcattct tatttttaac gatagcatta ttacttatcg cattattcta
      361 cgtttgcttt tataaaatta caaaataccc ataaatgttt gtgatagtgt gttggttaga
      421 tttattgatg cgctgtcagt aagtaattgt ttgcgaaaaa aatacaacct tgtacgaata
      481 caaagttgca ttaatataga atcatttatg ctttttaata tcacaaaaca aattgattaa
      541 actagctaaa atagtcaaaa ttggaatcaa gattacataa atatcataac ctctagatat
      601 tagtatacat aatataatta gcgaccctat tataattgct aaattataag ccaaactaat
      661 tttatatttc atttcaattc ccaccttaat agcatttatt aaaattctat aatgtttaca
      721 tagacttctt aacaaacttt aacccagcta agctaagtat tgaaataatt acacctccta
      781 atataaatat attacttaag cttagtaaag gtataattat acttatcagt cctaaagaca
      841 atgtatccgc tgcataattc gatgtagatg agatactaaa tactttcccc atcaaatgat
      901 ttggcgtttt tatttgaatg gcaactgatc ttgttagtcc ctctatagat tgtccaagtc
      961 ccaataatgt tgcacctata tataatatcg ccacacttgg aaacacatta ataatcgata
     1021 agccaattcc ccaaactaaa acaccaatac taaatttaaa gattaatcgc ttttctgaca
     1081 gcaaacccat aatcaatgac attaataaag atgctatacc taaacatgat gtagctagtc
     1141 catatacacc aacgccctct tttaatatat tggaaataaa caatggtaat acaacacgcc
     1201 aaaggccagt attaatcaat atgcaggcaa attggatgat tataataaat ggaatttctt
     1261 tagattgttt caagaattcc caagtttcag aaaaatcttc ttttgagtgt ctatcaatca
     1321 tgttgttatt tgtatatttt aaaagtgcat taaaaataaa tcctaaaaat agcaatatac
     1381 tacaaataaa aaagacgcca acattaccaa ctagtattac aatgacacca attaaagcag
     1441 gtaaaataat atttgagcct ctttgcaaac tatcgattaa cgcattactt gttgctaaat
     1501 gctcctcatc aataatttca ggaagaattg ccctaaacgc aggatccgta tagcagttaa
     1561 taatggtgat agctgtagat atggttagaa gcgtcagata atttaaattt gatgttattg
     1621 caagtaaagg aataattatt ataatcaaac ttagtataag atcagataga taaagtattt
     1681 tctttttatt atgtttatca gaatatgcgc caccgaaaat accaaatata ataaatggaa
     1741 gtgtttgact cataaccatc attgataatt ttaaagatga ttggtttgtc aattcaacag
     1801 taaaccaaat tatttgtaac gaaaacagca caaaacaact ccgacgtaag aaattaccaa
     1861 tcaataaata tgtaaagttt ctatttttca aaacttctaa atacaacata tttatcacct
     1921 ctcataaaaa taattgaatg catccaccag cttttttaga ccttcttcta aactctcttt
     1981 atccaaagcg caattaattc taatataatt tagtcagtta aatatcaatt atttcgaaat
     2041 atacatacta cttgaaacac catacataac ccccaaaatg actactcaga ggttatattc
     2101 tactaattat gattatatta aatatgaaaa tattatcaaa aaaatcaaat ttataacaaa
     2161 aatacacccc ttaaagttag gtctttcaat ccaacttttg gggtgtatat cattctcatc
     2221 atattctagg ttgtttttaa caaactaaat atagtgaatg caaatcaact attatttaaa
     2281 ttatgaatta ttttaattct ttcttctacg agccaataac attaatccag caattccaat
     2341 tatactacta aagatcaaac ctttttgcgt gctttctaaa cctgtttttg gtaattctgc
     2401 tcgttttttc tcttgattag ctactgattc tttagcaatt ttagattttt taactttatc
     2461 atttttatcc attgaatgaa ctgggccatt tggttttgct ctgtctttcg ataatcctgg
     2521 attgttagga tttactgggc cacttggatg agttggtctg ctcggcttct ctgggttttc
     2581 aggtcctttt ggatcttttg gtttctctcc accgaactct acaatcttat ctactggttg
     2641 ttttgtgatc tcttctgttg gttgaccctc gccaactttt tcacctgtta atgggttcac
     2701 tgtgattggt gttgtgattg tcttacttcc tggttgtcct tcttgtttca ctcgctcttc
     2761 accaggttgt aattttggat taaactcacg ttttgtttca aacggtatct ctactgtttt
     2821 tgtttctggt gtacccgttt ttggtccgtg tttaatcaca tcatccactg gctcttcgat
     2881 cacttttcct gtgtctggat tcttgattcc tggtttacct ggtacttttt ccgtttgatc
     2941 tgtgggtaag tttggatcaa agatatcttt atgaccttgc ggtattttct cgccaccgaa
     3001 ttctgttaat tcattaactg gatcttttgt gatttcttct ttcgattcac ctttactaat
     3061 aatttctcca gttaatggat tttttagtgt tggcgtcgtt attgtcttct cacctttttg
     3121 tccttctctt gttacttttt ctgtccctgg tgctaaatca ggattaaatt tacgttcttt
     3181 ct
//
LOCUS       DANJFZ010000182         3161 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.188, whole genome shotgun sequence.
ACCESSION   DANJFZ010000182 DANJFZ010000000
VERSION     DANJFZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.188"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            143..1318
                     /locus_tag="PWN45_002692"
     CDS             143..1318
                     /locus_tag="PWN45_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDL0624389.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1296..2486
                     /locus_tag="PWN45_002693"
     CDS             1296..2486
                     /locus_tag="PWN45_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0624390.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(2599..3108)
                     /locus_tag="PWN45_002694"
     CDS             complement(2599..3108)
                     /locus_tag="PWN45_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDL0624391.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1042 a          506 c          502 g         1111 t
ORIGIN      
        1 aataacgttt tattttgtat caattcatct atagattaaa attgctataa atgtaacgac
       61 tttgcgtctt tatttctttt ataatgccgt aaaataagtt acacttaaat aaaattttca
      121 aactaacgga gggtggctat taatggttac tcaaaataaa aagatattga ttattactgg
      181 ctcattcggt aacggtcata tgcaagttac acagagtatc gttaatcaac ttaatgatat
      241 gaatctagac catttaagcg tcattgagca cgatttattt atggaagctc atccaatttt
      301 gacttctatt tgtaaaaaat ggtatatcaa tagctttaaa tattttagaa atatgtacaa
      361 agggttttat tacagccgcc cagataaact agacaaatgt ttttacaaat actatggact
      421 taataagtta attaatttat tgataaaaga aaagccagat ttaatattat taacgtttcc
      481 tacaccagtt atgtcggtac taactgagca atttaacatt aatattccag ttgctacagt
      541 gatgacagac tatcgcttac ataaaaactg gattacgccg tattcaacaa gatattatgt
      601 ggcaacaaaa gaaacgaaac aagacttcat agacgtaggt attgatcctt caacagttaa
      661 agtgacaggt attcctattg ataacaaatt tgaaacgcct attaatcaaa agcagtggtt
      721 aatagacaac aacttagatc cagataagca aactatttta atgtcagctg gtgcatttgg
      781 tgtatctaaa ggttttgaca cgatgattac tgatatatta gcgaaaagtg caaatgcaca
      841 agtagttatg atttgtggta agagcaaaga gctaaagcgt tctttaacag ctaagtttaa
      901 atctaacgag aatgtattga ttctaggtta taccaaacac atgaatgaat ggatggcatc
      961 aagtcaactt atgattacga aacctggtgg tatcacaata actgaaggtt tcgcccgttg
     1021 tattccaatg attttcctaa atcctgcacc tggtcaagag cttgaaaatg ccctttactt
     1081 tgaagaaaaa ggttttggta aaatcgctga tactccagaa gaagcaataa aaattgtagc
     1141 aagtttaact aacggtaatg agcaattaac taacatgatt agtacaatgg aacaagataa
     1201 aattaagtat gctacacaga caatatgtcg agacttactt gatttaatcg gtcattcatc
     1261 acaaccacaa gagatttatg gaaaggttcc tttatatgca agattcttcg ttaaataatt
     1321 acgctaatca taaaaatttc attttaatgc ttattatctt atttttaatg gaatttgcga
     1381 gaggcatgta tatcctaagc tatattaatt tcttgccaac agttacatcc attgctgtag
     1441 caataacgtc tctagcattt tcaatacatt ttattgctga tgcatctaca aactttgtta
     1501 taggattttt actgaaaaaa ttcggcacaa aaatcgtttt aacgactgga tttattttag
     1561 catttacaag tttattttta gttatttggt ttccagcatc accttttgta atcatattta
     1621 gtgctatgat gctcggtatt gcagttagtc ctatttgggt cattatgtta tccagtgttg
     1681 aagaagataa acgtggcaaa caaatgggct atgtttattt ttcatggttg ttaggcctat
     1741 tggtaggtat ggtttttatg aatctactta tcaaagtgca cccaacacgc tttgccttca
     1801 tgatgtcttt agtcgtttta attgcatgga tattatatta ttttgtcgat gtaaagttaa
     1861 caaattataa tactcgtcca gttaaagctc aattgagaca aattgttgat gtgacaaagc
     1921 gccatttatt gttgttccct ggtattcttt tacaaggtgc cgcaattgct gccctagttc
     1981 ctatattacc aacatatgct actaaggtta ttaacgtaag cacaattgaa tatactgttg
     2041 caatcattat tggtggtatc ggctgtgcag tttcgatgct atttttatcg aaactaatag
     2101 ataaccgcag cagaaacttt atgtatggtg ttattctaag cggatttatt ttatacatga
     2161 tattaatttt tactctatct atgattgtta atattcacat cttgtggatt atcgctttag
     2221 ctatcggtct aatgtatggc atcttattac cagcatggaa tacctttatg gcacgattta
     2281 tcaaatcaga tgaacaagaa gaaacttggg gtgtttttaa tagtatccaa ggattcggct
     2341 caatgatagg gccattattt ggcggattaa ttacacaatt taccaacaat ttaaacaata
     2401 cattttattt ctcggcatta atctttttag tcttagctgt attttatgga agctacttta
     2461 tagtaaatag agaaaaagct aagtaaggtt aaaacgtatt ttaaaataaa gaaaatggtc
     2521 atttttatta cattcaataa aaatgaccat tttcttatta attcaaacat actacgatta
     2581 acaatactat tatgttactt aagctaattt aaacgtttca ataactttcc atttatcttc
     2641 gtcatcgtca aaacgtaaca aagttaattc atcgataatt tctttatggt ctaccccagc
     2701 taatgctact tgaccaaaaa tatcttcgaa ttcttggcta gatagtcctt gtgctattgt
     2761 aaagtgtggc acaaaaacat gttcagcttc tccatagaaa tcttctccat taaagcgatt
     2821 aaacaattct tctaagtcgt ccgtcttcgc aactttaaag taaatcacat tgttcgttgg
     2881 tttgaagcta gaagcttttg tagcatgaac ttctactgct ggtataccat taatacgagc
     2941 tctaacctgt tcaatgacag aatctaaatc accatcttta atttcaaatg gcgctttaat
     3001 tgtcacatgt ggtttaattc gtgaatactg tttatcatat cttttacggt aagaatccac
     3061 cgcttcttga aatgactttg atggaattaa tgctaatcct aaaatcatct gaattcctcc
     3121 tttgtttaac ctattgctat tatatcaaat ttctttgaaa a
//
LOCUS       DANJFZ010000183         3147 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.189, whole genome shotgun sequence.
ACCESSION   DANJFZ010000183 DANJFZ010000000
VERSION     DANJFZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.189"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            402..767
                     /locus_tag="PWN45_002695"
     CDS             402..767
                     /locus_tag="PWN45_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDL0624392.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            949..2226
                     /locus_tag="PWN45_002696"
     CDS             949..2226
                     /locus_tag="PWN45_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDL0624393.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2630..2986)
                     /locus_tag="PWN45_002697"
     CDS             complement(2630..2986)
                     /locus_tag="PWN45_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDL0624394.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         1083 a          449 c          524 g         1091 t
ORIGIN      
        1 acatccaatc actccttgtt aaaataaaca atattatgaa gtgtaatagt tgtcaagtac
       61 tgatttcatc gcaattattg aattgtctat aattgaattt aaatgttaaa taatgcaaaa
      121 tatatcgata tcttagtatt ttcatcggta attactgata tgactgattt tttaaattga
      181 aaaaaatgta attgtatcat ccttaattgt taaaagtagt caattatttt gatagatagt
      241 aaaataaaat ttgttattaa attttttaaa agcatcgtgt atgtaattcg atttctataa
      301 taatattgag aataaaaaaa tttgttacaa tctggtaaaa actctttatt aaataaattg
      361 attcgttcat actgaataga taagcaaaaa ggagttacat aatgaagaag aaaaaaaacg
      421 cgattaaagt tatcattata ttatttattt cattaattgt tgcagtagca tttttctttg
      481 gtctgaaaac atatcaaggc cataagaata ttcaactaat tgacagttac ttggaagaga
      541 aaaatctaaa agataaaatt aaatcagaaa aaacagagta tagtgctaaa aaaggtctct
      601 tctataagga agttacattt aaagatgaac ctggtgtaac ttatgttgtg caaccaatca
      661 gtactaataa aggattattt gttgaaggct ttgatacaga gactaagaaa agtttgaaaa
      721 ctgcaaagca taaatatttc aatcaaaatt ataaaccatc aaaatagtga aacgttaaac
      781 tggtgaagca gtttagtgcc ttcaatttta attatttgag gtaaacagtt tttgtatgga
      841 aattagtgat ttttgacatc tataaaagat aaatatttta attttttata gataattatc
      901 aattttctga tataattgag tgatgtcaga tgaaggggag tttgtactat ggctctattc
      961 aagagaaaaa ttagcctacc tatgcaagtt gttattgcat tagtgttagg tgttgtcgta
     1021 ggacttttat tatatggaca agaaaacgta gcaaattaca ttaaaccatt tggtgacgta
     1081 tttttaaatt taattaaaat gatcgttata ccagttgtat tttgctcact agcgctttct
     1141 atttcgaacg ttggggaatc gaaaactgta gggcgttatg gctggaaaac aattttatac
     1201 tttgaaatta ttacaacaat cgcaataggt ttagggatta tcttcggtaa cctatttaaa
     1261 ccaggtgctg gattagaccc aacaaaatta cctaaaggtg atatttctaa atatcaatca
     1321 actgcacatg cagcagaaca atctacatat ggaaatcatt ttattgatac cattgtacat
     1381 attattccga caaacttttt tgaagcttta aataagggtg aattattacc tattatcttc
     1441 ttcgcagtat tctttggatt aggattagct gctgtaggta aaaaagcaga accagttaaa
     1501 gaatttttaa gcggatcgct tgaagctgtg ttctggatga ttaataaaat tttaaaatta
     1561 gcaccacttg gagtgtttgc attcatttgt actacaatta ttacatttgg tgcatccgca
     1621 ttattaccac tattaaaatt agtattagtt gttgtctttg caatggtgtt ctttgtattc
     1681 gctatactag gactagttgc atggatgtgt ggtattaata tcatgaatat tattagaatc
     1741 ttgaaaagtg aattgctttt agcattttct acatcaagtt cggaagctgt acttcctgta
     1801 atgatgaaga aaatggaaaa cttcggttct ccaaaagaaa ttacttcttt tgttatacca
     1861 attggttata cgtttaactt agatggatca gcactttatc aatctattgc agcattattc
     1921 gttgcacaga tgtatggaat gcacttaaca ttatcagagc aaattgtgtt gatgttaaca
     1981 ttaatgatta cgtctaaagg tatggcggca gtaccaggaa cttcaatcgt tgttttatta
     2041 acaacattag gtgccatggg cttaccggca caaggtttag cattaattat tggtgttgac
     2101 cgtatcttag atatggtacg tacatgtgta aacgttattg gtaatgcatt atcaacaatc
     2161 gttatagcta aatgggaaaa cgtatatgac aaagcaaaag gtcaagaata tttaaaatca
     2221 atttaaaaaa tactatctga catttaaagc cccttacaac ctttggttgt taggggcttt
     2281 tatgcatgcg ctaaagcgca ggcgaacgga agcgtagtga cctcaggttg ttagggactt
     2341 ttatgcatgc gctaaagcgc aggcgaaccg aagcgtagtg acctcaggtt gttaggggct
     2401 tttatgcatg cgctaaagcg caggcgaacc ggagcgcagt gacctcaggt tgttaggggc
     2461 ttttatgcat gcgcaaagcg caggcgaacg gcaagtggag ctattcaata tgaaagtatt
     2521 tcaaatataa aaagcagcag taaaatgatt ccaaattgga aatcaactta ccgctgcttt
     2581 tactcgtata taccttgatg gcaactaaaa tttgtaattt aaattacaat tagtgtttca
     2641 tgtgcatttt gcccatgtca tgaattttca tcattaaacc atgtggtaca tcgtcatggt
     2701 caactttttt aactttaaca attttatctg aatctgctaa tttataaaca attacaaaat
     2761 cacctttttt aactttaaca tctgaatctg aaggtaattt aacatttttt tcaatacttt
     2821 catctttttc actaatcatt ttttcaatag tagtattatc tttcataatt ccatagtatg
     2881 tatctttctt tccgccagtc atcatcatga ttactaatac aatacataca attaacatga
     2941 tggcgattaa actaatacca agaccaaaac cttttttctt tttcatggtt catcaatctc
     3001 cttaaatgta gttaattctg ttatacctaa aagtgcaaca gtttgcaaat gcagtattta
     3061 tattacataa aattaattta aaaagacaca ccctaatcac gttaaatgtg aataaagtgt
     3121 gtctaaaaaa caatacattg caaattg
//
LOCUS       DANJFZ010000184         3125 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.190, whole genome shotgun sequence.
ACCESSION   DANJFZ010000184 DANJFZ010000000
VERSION     DANJFZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.190"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(55..243)
                     /locus_tag="PWN45_002698"
     CDS             complement(55..243)
                     /locus_tag="PWN45_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624395.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="PWN45_002699"
     CDS             complement(826..1821)
                     /locus_tag="PWN45_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDL0624396.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="PWN45_002700"
     CDS             2273..2854
                     /locus_tag="PWN45_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624397.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DANJFZ010000185         3096 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.191, whole genome shotgun sequence.
ACCESSION   DANJFZ010000185 DANJFZ010000000
VERSION     DANJFZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.191"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(10..762)
                     /locus_tag="PWN45_002701"
     CDS             complement(10..762)
                     /locus_tag="PWN45_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL0624398.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            813..1142
                     /locus_tag="PWN45_002702"
     CDS             813..1142
                     /locus_tag="PWN45_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624399.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(1131..1346)
                     /locus_tag="PWN45_002703"
     CDS             complement(1131..1346)
                     /locus_tag="PWN45_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL0624400.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1362..1625)
                     /locus_tag="PWN45_002704"
     CDS             complement(1362..1625)
                     /locus_tag="PWN45_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624401.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(1622..1796)
                     /locus_tag="PWN45_002705"
                     /pseudo
     CDS             complement(1622..1796)
                     /locus_tag="PWN45_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1759..2472
                     /locus_tag="PWN45_002706"
     CDS             1759..2472
                     /locus_tag="PWN45_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0624402.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            2488..3096
                     /locus_tag="PWN45_002707"
                     /pseudo
     CDS             2488..3096
                     /locus_tag="PWN45_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT          988 a          521 c          473 g         1114 t
ORIGIN      
        1 ttcctccttt taagatgttt gtttttctcc taaaaactta ttaacaaagt attgttgtcc
       61 tttgcctgtt acttttggcg tcttactaat tgatgtgtga ccgtccgaat gtgtgattga
      121 tgtttcttta atttcgaata actcacgttc cattgaatac tgtgtaggca tgttataatc
      181 cacacccttg cgtttaataa ggaatccgtt ttgacgtaac cactcaaaca atctgcgttg
      241 cccgatgttt acaccgtttt gtttaatgat ctttgctaac tctccaacta aaattgatgt
      301 cttagtagta gctactgcat ctgcaaatac aatttttggt ttatcacgtt caatctttgt
      361 ttctaattga ttgattgtgt tgttagcaat ttttaaagca cgttgcataa tcatttctgg
      421 gctgttccat gctttttcaa cttggatgaa gtattgtctt gcacgtttac caggttcact
      481 acgttgaatc attgcgattt cttttgcagt gtctagtgtg agtgcgtggt caatatagtg
      541 tgtcatattg ccttgagctg ttgctctttt ttgagcgata gctgtgtaat ctgtattttc
      601 ttcaaatccg tatttaagca ttcttggaaa ccaatcttta tatgctgtct taacttctaa
      661 tgcttgatga agttctcgac cgctgattgc gatttctcca ttttcttttt cttgaatatt
      721 gaacatttct ccgatgttcg aatttgtttg taatgcttgc atattgttta tgctcctttc
      781 tgctatactc ctattaagga ggtgaatgac ttatgactaa tgaagctaaa tttgtccttt
      841 tacaacttta ttcaatttac cttgatagaa ttgacgaagg tatgtctaaa cgttctgcat
      901 cttatttcgg tagtgatgaa tcctcattta acgctttctt tttagatttt aattttgaag
      961 actatatcga tgcagttctt gaattaaagc atagagattt tgtaattgct tctgctgaag
     1021 atggcggttt tcttgagatg gctctttctc gagaaggtat cgcctactca gaatcagaat
     1081 ccaaaaaaga ttacaaaaca cttatggatt taattagaga tttgaaaaaa ttaataatct
     1141 aaaatccaat catctgctat taaatcgtct gcgctaggct gccaccttcc ggcggcggtt
     1201 tgtctttttt tcttatagtg tctagatacg actaggcatt gataacgctg caaattagtt
     1261 ggtaatatcc catacacatc ttgattctct ctccttatac ttattccttt ctccatcgct
     1321 agcttcgttg cttcttgaat gttcatttgt tattcctcct attaagatgt ttgtttttct
     1381 ttaaatgcta aaataattga tttcttttta tcattcgtaa atacgaaatt ttcgtattca
     1441 ttacctaaaa aaatatcatc atatttaaca ttaaaagcac tcatatactt agaaagtaaa
     1501 ctatctttaa tgtttgtaga gtctttttcc atattttgaa ttgtacgtga tgagacctta
     1561 aataaatctc ctaactcttt ttgagtcaat ccgtaatcag tcctcaactc ttttaatgtt
     1621 ttcatgttgt caccgccttt cgtaaaccta atataatacg aaattttcgt attgtcaaca
     1681 ttaaatacgt ttttttcgta aaaaaacttt actatgatat gaaaatttcg tataataaga
     1741 aaaaaggagg taagtaatat gaacaaagaa agaaatatta ttatagccaa aaacattaga
     1801 aaatttctca acgattcaaa tatgtctcaa aagaaacttg ctgaactcat taacataaaa
     1861 ccatctactt taagcgatta tttaaattta cgttccaacc cctctcacgg cgttatacaa
     1921 aggatagctg atgttttcga ggttggtaaa agcgacatag atactacata caaagacgat
     1981 aacgacatca cttccatata caacaaactc acacctcccc gccaagaaaa cgtacttaac
     2041 tatgcaaatg aacaattgga agaacagaat tctaaaggag ataacgttgt agatattaat
     2101 tcatataaac aggagaaaac tccagttaac gtcaatggtt gcgtctctgc tggtgtagga
     2161 gaacgtttac acgatgaaac gctatttact gaaatggtta aaggacctat ccccacacac
     2221 gatttagcgt taaaagtaaa tggtgattct atggaaccta tgtttaaaga tggcgaaatc
     2281 atatttgtgg agaaaactca caatataaag aatggacaaa ttggtatatt catcattgaa
     2341 gaagaagcgt acgttaagaa agtctttgtt gaagatgata gattgactct agtttcacta
     2401 aataaagatt acgacgatct acacttttat agaaatgaaa gtgtgaggtt aattggaaaa
     2461 gttattttat aaaaggagca cttgcaaatg aaaaaatatg atattgcagt cttagacttt
     2521 gaaactatga atgaacatat gaacagccct tgcgaagttg ctgtatcttt aattaaggat
     2581 ttatcaatag taaaagttta ttcatcttat attaatcctc ctaataatag atataacttg
     2641 aaaaacgcta aaatacataa aatacctgaa gatgtcatat taaaagcacc taaatatcca
     2701 gatatttacc aagaaattct ctatctttta aaagaatcac atttaattat tgctcataat
     2761 gcactttttg atatttcagt attaaaaaat actaataatt attatgactt acctgttcca
     2821 aacttcatgt atgtcgatag tataaatatc tttagaagct tccacgcaat ctctagtttt
     2881 aaattagaaa atttgtgtag cttatatgat atcgataaag aaaaattaca ttctgctaaa
     2941 tttgacgtgc tagctttatc gaagatgttg atatcactcg ctaaaaacaa tcagcattaa
     3001 agtgtattaa aattaataca ttatatgcct aagcaataca ttagatttag caaatattct
     3061 aactctccaa ctaaactttt cgattcagga tttcaa
//
LOCUS       DANJFZ010000186         3058 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.192, whole genome shotgun sequence.
ACCESSION   DANJFZ010000186 DANJFZ010000000
VERSION     DANJFZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.192"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..557)
                     /locus_tag="PWN45_002708"
     CDS             complement(<1..557)
                     /locus_tag="PWN45_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624403.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMD"
     gene            complement(544..1374)
                     /locus_tag="PWN45_002709"
     CDS             complement(544..1374)
                     /locus_tag="PWN45_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624404.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(1367..2353)
                     /locus_tag="PWN45_002710"
     CDS             complement(1367..2353)
                     /locus_tag="PWN45_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624405.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(2524..2709)
                     /locus_tag="PWN45_002711"
     CDS             complement(2524..2709)
                     /locus_tag="PWN45_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624406.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(2657..3001)
                     /locus_tag="PWN45_002712"
     CDS             complement(2657..3001)
                     /locus_tag="PWN45_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624407.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
BASE COUNT         1093 a          527 c          414 g         1024 t
ORIGIN      
        1 aaatccatca cttgattacc gttcaaagca tccaatgatg caactggttc gtctgcaatg
       61 attaatttag gctccaacat gagaacgctt gctatgtata cgcgttcaag ttggccccca
      121 gaaagttgga aactatattt atttaatata tctttgcttt gtaaattaac ccacgacaaa
      181 gccttatcaa ctttggacaa agcctcttct ttactacctt tataatgctt acgataaatc
      241 gcagttaact gtttacctaa tttagtatgg tcgttaaaac tttctgcata attttgagaa
      301 atatagccaa ttgtatgacc ataatattga ctcaatctac taacattttc cccatcaaat
      361 tggtacgaat catacgtgca gcttaaatca aatggtaaat attcaagtaa agctttagca
      421 atcaaacttt ttccagcgcc gctctctcca atcaaggcat taatctgttg actaaaaatt
      481 ttcaaatcaa tccctttaat aagagatttc tcactagtat tctttattgt taaattttgt
      541 atatcaatga gactcatcat attcaccccg ttgtttcagc aatctatctc ttagtgcatc
      601 accggttaaa ttaaaaatta aaatagttat agcaatgact gaagcaggtg caatcaacat
      661 aattggatga gacgaaataa aatcacgacc ttgttgcaac atagcgcccc actctggtgt
      721 tggcggttgt gcacctaacc caataaatga tagtgaactt atatatagaa tgattttacc
      781 gaaatcaacg accatcaaaa cgataatagc cggtataatt ttaggtgtta aatgacgtat
      841 taatattgtt cttgttggta catgaaataa ttgtgccatt tttatataag gcttattcat
      901 ttcgctatta actatacttc tagtcaacct tgtgtaattc atccatttta ttaatgtaat
      961 tgagataact aaattccata aagatggttg aaaaaaactt gctaaagcaa tcatgatgat
     1021 aaattctgga atacttagac caacatcaat aaaccttaac actaatcgtt caatccaccc
     1081 ttttttgtat ccggcaaata gacctagtgt aacacctatg acaacgatag ctattaatgt
     1141 taaaacagta acaaacaatg ttgaacgtgc accgataata attcgggtaa ataaatctct
     1201 cccataatca tcagttccta ataaatgcaa ccaactaata ggttcaaaag tttgtgataa
     1261 attgactttg gttgcatttt cactactgac aaagaattgc agtacaatta ccacaaaaat
     1321 aaatgcaacg aatacaaaaa atatcaggtt attctttgaa aatattttat gcatgacggt
     1381 cactactttc tgatatcaat ggtgtattgg ttttgatttt tggatttcct aattgtaaac
     1441 gctgcttcgg atcaagtaat aacgttaata aatcagcaat cgtattgata ataacaacga
     1501 agaagccaat aaataacacg catccttgaa taacaggata atctcgagat ttaatactat
     1561 ccattaatag ataaccaata ccaggtatat caaataaatt ttcaatcact acagtaccac
     1621 ctattagact gccaagtgaa atccctagta atgggataat cggcaaaatt gttggtttta
     1681 gtaaatcatg aattaaaata taacgttcat tcataccgcg taatcttgat gcttgtacga
     1741 tattactttg caataacatc aataaattag aacgcactaa acgaatgatg tatgcacaca
     1801 tacctaaaga tagcgtgatt acaggtaata taaactgact tagtataacg ctatctatat
     1861 tcattaaatt tgtgacaata aataataaaa taataccgat aaagaacgct ggtaaactaa
     1921 tcgatagtgt tgagatcact ctaatcactt tatccgtcca cttatgaaat cgtttggctg
     1981 ctataatgcc gagcggtata gatatgcata acgacactac taatgttgaa aatgatatga
     2041 gtaatgttat gggtgcatag ttgaataata tctgtgttac cggttctttt gattcaaaac
     2101 tttttcctaa attaaaatgt aataaatgat tcatccaatg ccaccactgt accaataaag
     2161 aatcatttaa tcccaattta tctttggttg catttatttg ttccgtcgac acttgtgcta
     2221 catcaagatg taatatttta tcaacaggat tgcctggtga taatttcatt aaaatgaatg
     2281 taagtgtaga aataacaaat aaaacaacta tcatttgcat cagtctatac aacatagact
     2341 ttattatgaa cataatagtc cccctccttg tgtaagttac taacactttc tttttacatg
     2401 agaatggcgc atgtatatgc aacttacata ttaagaacta acgttcatta tagtattatc
     2461 cataaagaaa ttgaagtata tttaattttt taacaaaatc attataaaat ataatatttt
     2521 gaatcaagtc aaccatgtaa aatataaaaa agtcaaaaca aaaacaacta tagcactgta
     2581 ttccatctct ttcgaaataa ttgttactgc agtgtaactt aaaagtcgat gattttgtgc
     2641 atatagttgt cgaatattat tttttatctt tacggcgaag ttcagcgccc tcatagccgt
     2701 atttttcaat ttgcttttct aatttacgcg cttttctttc tttacgccaa tttctagtaa
     2761 aataccataa tagaaaacta attaataaac tcataatcgc taaaaatgca gcgtatccta
     2821 ataatggttg atattttata tcttgaaaat ttggaataaa aaatgcaagc acacctaata
     2881 taacaaatgt aattactgca gatacaaacc atttatttaa aactaagcaa cagaatattg
     2941 ttaataaaat cattattaat gttgtgatcc ataaataatt aggcatatcg aataatgtca
     3001 tattcattct ccttttattt cattactttc cttgtataca ttttattata aattttta
//
LOCUS       DANJFZ010000187         3057 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.193, whole genome shotgun sequence.
ACCESSION   DANJFZ010000187 DANJFZ010000000
VERSION     DANJFZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.193"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..282)
                     /locus_tag="PWN45_002713"
     CDS             complement(<1..282)
                     /locus_tag="PWN45_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HDL0624408.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAID"
     gene            544..729
                     /locus_tag="PWN45_002714"
     CDS             544..729
                     /locus_tag="PWN45_002714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624409.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(952..1530)
                     /locus_tag="PWN45_002715"
     CDS             complement(952..1530)
                     /locus_tag="PWN45_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0624410.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="PWN45_002716"
     CDS             complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="PWN45_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDL0624411.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2164..>3057)
                     /locus_tag="PWN45_002717"
     CDS             complement(2164..>3057)
                     /locus_tag="PWN45_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDL0624412.1"
                     /translation="SLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQ
                     SLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDST
                     YKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQ
                     IPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAK
                     NQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPS
                     GKNAELISEKILKGMGVLNDQ"
BASE COUNT          965 a          504 c          368 g         1220 t
ORIGIN      
        1 atcaatggca cgatataaat agctccattt tccttttatt ttgatgtacg tctcatcaat
       61 acgccatttg taataagctt ttttatgctt tttcttccaa atttgatata aaattggggc
      121 atattcttga acccaacggt agaccgttga atgatgaacg tttacaccac gttcccttaa
      181 tatttcagat atatcacgat aactcaatgc atatcttaga tagtagccaa cagctacagt
      241 gataacatcc ttgttaaatt gtttatatct gaaatagttc atacagaaga ctcctttttg
      301 ttaaaattat actataaatt caactttgca acagaacctc atttactata tgggcatctc
      361 tttcattatt aatacaaagc tttgtatctc cgatattagg caaaggtgta aacgaattag
      421 gtgatactat aggattcaac attatattaa tcgtttcttt aataatgatt atcttcttat
      481 tttttataaa tcgtagtaaa aatatctcct atgattatat aaaagttcaa agaggtgaag
      541 ccaatgaata aattagaaaa atggtctttg gtatttatta tttttacatt tctctcttca
      601 ttagcacttt tatatttcaa atataatttt agttcggtta tgccagtcct aagtctttct
      661 attttaacta tactaatatt gttgacgagt attattactg tagtttcagt taatgatgaa
      721 gatacttaaa acgttcatta gtgaaacagg tattatatta aaaaagcgta cgaatttgta
      781 cgcttttttc ttgtgttatt attaaaatct cataacagga aaatattatg agattttaga
      841 cgctgtcaga acgtctcttt tacatcgagt gacattgaat caaagtcttt gtatgttaat
      901 gatatataaa attatttagt agattcatta tagtaagcta gattagttgc tctacttgtt
      961 tttattctct tttgcctcta atttattgag ataacgataa atagttgtac gtgaaacttg
     1021 ccactgctct gcaattgttt taattggcgt accattatca taaagtgtct taagtaagtt
     1081 taaatcttgt ttatttaatt tttcaggtct acctccatat cggcctctag cacgtgctgc
     1141 aattcgaccg gctgatgatc gctctaaaat taaattacgt tcaaactctg caaatgctgc
     1201 gaataaatga aataataatt gtcctgtaga acttgattta tccatcgtta tattttcttc
     1261 taaactatga aaactcacac ctcgttcgtt aagttcatta actaacgtga ttaaatcctc
     1321 catgttccgt cctagtctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     1381 ttcaattgct ttatctaaac caggtcgctt actttttgaa ccactcatat ggtcattaaa
     1441 tatcttttca caaccatatg tatttaatcg atcctcctgt aaattcaaat tttgtaatcc
     1501 agttgaaact cttgcataac ctattttcaa aatacagacc tcctatatac acctttagtg
     1561 taccataact tgattacgag gtcttttgtg aacatagatt ttttgttggg tttttgaaca
     1621 cataattcta tgtaatactt acttatttag acaaaaaaca agagtgtaca gaaaatggtt
     1681 attttctgga cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt
     1741 aataagagtc aagtatggtt tacatatgta ttatgtttga aacatacctc taattacttt
     1801 ttactaatat catttaaaat gtctcgcaat tcttcaattt ctttgttatt taattcttca
     1861 tttttcgcaa aattcagcac taaacttttc atatcccctc catacagttt attaagaaag
     1921 gttttagcag ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata
     1981 ttttctgatt tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta
     2041 atcgttttat cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat
     2101 actgattttt tatcccatat tatattcata acatcccatt cagccataga tatttcaact
     2161 tgcttattgg tcatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc
     2221 atttttccca gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt
     2281 tgtaattacg taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc
     2341 tgttttccca tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg
     2401 attctttgct tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac
     2461 ttgttcaaga ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa
     2521 attttcatta ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg
     2581 attactaata cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg
     2641 ttccttattc caagactcga aaagataatg tttatgattc catgacatac gtgaattttt
     2701 atcacttata atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc
     2761 aggagaatat ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat
     2821 gctgtacata acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg
     2881 gtaatcgctt tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa
     2941 ctggctttga attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt
     3001 tgactgcttt ttcagatcgg cttcttttat attaattaat ctacttttga gtaatga
//
LOCUS       DANJFZ010000188         2969 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.194, whole genome shotgun sequence.
ACCESSION   DANJFZ010000188 DANJFZ010000000
VERSION     DANJFZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.194"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            226..780
                     /locus_tag="PWN45_002718"
     CDS             226..780
                     /locus_tag="PWN45_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDL0624413.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            complement(1015..1983)
                     /locus_tag="PWN45_002719"
     CDS             complement(1015..1983)
                     /locus_tag="PWN45_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL0624414.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(2260..2412)
                     /locus_tag="PWN45_002720"
                     /pseudo
     CDS             complement(2260..2412)
                     /locus_tag="PWN45_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(2412..2903)
                     /locus_tag="PWN45_002721"
     CDS             complement(2412..2903)
                     /locus_tag="PWN45_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDL0624415.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
BASE COUNT         1054 a          461 c          363 g         1091 t
ORIGIN      
        1 taaaaagtca gcccgaaggc taacttacga atagatgaaa atttgaacac attgctgtgt
       61 ctaaaatgat tatagcataa ataacgaata tttccagctc aaaattaata tattgtaata
      121 ataatatttt atatctttgt taataattat ttaattgatt tacataaata ataattgtaa
      181 aattactttg taatcgattg caaataaatt ataggagaaa ataaaatgaa taaaaaacta
      241 ttaacaaaaa cattgatagc aagtgcttta gttttaacaa cagtaggttc aggttttcat
      301 tcttcttcaa attataatgg tattaataac gttgcaaaag ctgagcaaac gacagataac
      361 gaattgtgga aaaatgtaag agacgcttta aaagaagcga atattatcga taaaacagat
      421 aatgaaaatg tcaaagtgac ttataaatta gcaaatggcg gtgaaaacag tattgaaggt
      481 accgccaatt taaatgaact tagcaattct aataacaaac cgatcaatag cgatagtgta
      541 aaaagcattg atttgtcaag gaaaaatccc aatggagata gatttgacgc aaatgatgca
      601 tggaaaaaat taactgataa attaaaagaa aagcagattg tgaaagacgg tgatacagta
      661 actattcata gtaaagacga aaaagaccct aaaatctcag ctaaagtcgg tgaagtctat
      721 aatgataaca aacgattgat gttgtttaaa aaggatatag ataaaatcac tataaactag
      781 ttatagataa agacaggtta cttttttaat gtaacttgtc tttttttaag aggcagtatc
      841 tcaaatatta atttataatt tctaaatcta tatctatact ttatctattt ctaaatctat
      901 atctatactt tatctatttc tatatacaca ctcatccctc actgcaacac agggcgtttc
      961 tcagcgtctt aaaataaaaa aacgccactc gtaagtgaca ttaaaaaata tcttttataa
     1021 catatccagt atttttgttt gatctggaaa attcattttt gtatataggt catctatagt
     1081 aattgtatag aaagaatgtg aataattact tattagttta tccatatttt tcatcaattt
     1141 tatgtaatca tgtttattca aaaatagaca tagtgaaatc aacaaatcga atacataact
     1201 accattcatt gtaggaacgt attctttatc gtatattttg ttataattgg cgaatatatt
     1261 agctctactt ttagccctgt ctattttata atcatacaat ctttcttcat gcgcacacac
     1321 gttacgaaac atatgtgctt gttgtagtat actgtctaca tctgatggag ttatttgaac
     1381 acgtgtttta taatctcttt ccaatttcct tttatagtct ttagcaactt ccaatcgaag
     1441 atcatcatcc aaattggaat acatttttga aacattacct aaagttaaat aattcaccaa
     1501 tatccatagt ggcactccat tatgagtatt aatataatgt tttaatggtt tattttttct
     1561 attactcata accgagctaa atgtagcaac cattttcacg atgctatctg tcttacttgt
     1621 gtcagatgaa taatttttaa agtataaata tgaatgtggt tctctatatt tttcgctaaa
     1681 ataatatgaa attcttgatt taatatgagt ttcaaatact aacaaatact ctaataaaac
     1741 attcctaaat tttctatcta gtttgtataa agaaaagact tctttaaaat gagtaccttg
     1801 cttatattta tcaggaacca aaaaattacc attaacatct agttctaaaa ataaatcttt
     1861 ataaccattt atgatattat aataattttc attttctaaa tctcttttag cactactcgg
     1921 tacttccatt cctcttcttc ttagaatttt caattgttta ttatgacttt caaatggctt
     1981 cataaattta cccctataaa agtagccata acccgaatag agttatggct agatcgttat
     2041 atatataata catttaattt tatcggttgt aaataaataa gaaataacaa aaagagatat
     2101 tttacacaat tacctccttt attttattgc tactcctcaa acccaccaat attatcaata
     2161 aacactggtg ttgttacatt taagtctact ttctcagtaa ataagctatg gtatcaaact
     2221 aattagtatt aatttataaa tagcatagct tcattttctt caatcctcta acggtatatc
     2281 atccacaatc acagtatgat tatggttagc gttagataca tcttgcactg ccttatctaa
     2341 ttcctcatca tctccgtccc attcaccaat attaatgaat ataggaatat tcccgttaat
     2401 atcatgctta tctatgaaag ttctttttta acttgttcta aatgcttatt aaactctatt
     2461 cgatgtaatt cttctcgctg gttttgtcgt tcttgacgct ttctactttc aactttataa
     2521 atatctactt ttaaacgttc aatgactcct tcatttttaa ctttcccact acttaacatg
     2581 tacctcataa catcctgttg ttcattttca gaatagtttt tgatgatatt tttaaacaaa
     2641 tttagcctat cctgagctgt tttcatgtaa tattcaagtt tatctttctc ttctataata
     2701 taaagtacta atttatctaa tggatatgat acagatacca gcatatcgtt atcattcgtg
     2761 atcacatggt tcatgttcaa gtgatataag ctttctattt gtgatctcat cgctttaatt
     2821 ttgctattaa tgactttcgg attgtaatgc attaaaagtt ctacttcagt gattttatca
     2881 ttgctttcaa agtcagatac caaataacca cacctttaca tatcgaattt aatatttata
     2941 agtatctgaa aaagggataa atcaagtta
//
LOCUS       DANJFZ010000189         2843 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.195, whole genome shotgun sequence.
ACCESSION   DANJFZ010000189 DANJFZ010000000
VERSION     DANJFZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.195"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            190..2232
                     /gene="lip1"
                     /locus_tag="PWN45_002722"
     CDS             190..2232
                     /gene="lip1"
                     /locus_tag="PWN45_002722"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDL0624416.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
     gene            complement(2679..>2843)
                     /locus_tag="PWN45_002723"
     CDS             complement(2679..>2843)
                     /locus_tag="PWN45_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine export
                     protein"
                     /protein_id="HDL0624417.1"
                     /translation="AYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGK
                     QPYKLNINAY"
BASE COUNT         1133 a          435 c          530 g          745 t
ORIGIN      
        1 agcgtataca ctttaaattc tctttggaga atatattttt taaatacaaa tgtaaacgct
       61 ttctcgtcaa attaaacaat agaaaggatg gtcattatga gtgcttggtt aagtaaatta
      121 tttgagttta ttcctcgaat aattatcaat ttgtttatct aaaataaaaa aatagaggtg
      181 ctgacaatga tgaaaagtca aaataagtat agtattcgta aatttagtgt aggtgcatct
      241 tccattttaa tagctacatt actattttta agtggtggac aagcacaagc agctgagaag
      301 caagtgaata tgggaaattc acaggaggat acagttacag cacaatctat tggggatcaa
      361 caaactaggg aaaatgctaa ttatcaacgt gaaaacggtg ttgacgaaca gcaacatact
      421 gaaaatttaa ctaagaactt gcataatgat aaaacaatat cagaagaaaa tcatcgtaaa
      481 acagatgatt tgaataaaga tcaactaaag gatgataaaa aatcatcgct taataataaa
      541 aatattcaac gtgatacaac aaaaaataac aatgctaatc ctagcgatgt aaatcaaggg
      601 ttagaacagg ctattaatga tggtaaacaa agtaaagtgg cgtcacagca acagtcaaaa
      661 gaggcagata atagtcaaga ttcaaacgct aataacaatc taccttcaca aagtcgaata
      721 aaggaagcac catcattaaa taagttagat caaacaagtc aacgagaaat tgttaatgag
      781 acagaaatag agaaagtaca accacaacaa aataatcaag cgaatgataa aattactaac
      841 tacaatttta acaatgaaca agaagtgaaa cctcaaaaag acgaaaaaac actatcagtt
      901 tcagatttaa aaaacaatca aaaatcacca gtagaaccaa caaaggacaa tgacaagaaa
      961 aatggattaa atttattaaa aagtagtgca gtagcaacgt taccaaacaa agggacaaag
     1021 gaacttactg caaaagcgaa agatgatcaa acgaataaag ttgccaaaca agggcagtat
     1081 aaaaatcagg atcctatcgt tttagtgcat ggtttcaatg ggtttacaga tgatattaat
     1141 ccttcagtgt tagctcatta ttggggcggt aataaaatga acattcgcca agatttagaa
     1201 gaaaatggtt acaaagctta tgaagcaagt ataagtgctt ttggaagtaa ctatgaccgc
     1261 gcagttgaac tttattatta tatcaaaggc ggtcgtgtag attatggtgc agcacatgca
     1321 gcaaaatatg gacatgaacg ttatggaaaa acatacgaag gaatttacaa agactggaaa
     1381 ccaggacaga aggtacacct agttggacat agtatgggcg gtcaaacgat acgtcaacta
     1441 gaagaattac tgcgtaatgg taatcgtgaa gaaatagagt atcaaaagaa acatggtggc
     1501 gaaatttctc cactattcaa aggtaatcat gacaatatga tttcatcaat tactacttta
     1561 ggaacaccac ataatggtac acacgcatca gatttagctg gtaatgaagc tttagtgaga
     1621 caaatcgtat ttgatatcgg taaaatgttt ggtaataaaa attcaagagt agacttcggg
     1681 ttggctcaat ggggtctaaa acagaagcca aatgaatcat atattgatta tgtcaaacgc
     1741 gttaaacaat ctaatttatg gaaatcaaaa gataatggat tttacgatct gacgcgtgag
     1801 ggtgcaacag atttaaatcg taaaacgtcg ttgaacccta acattgtgta taaaacatac
     1861 actggtgaag caacgcacaa agcattaaat agcgatagac aaaaagcaga cttaaatatg
     1921 tttttcccat ttgtgattac tggtaactta atcggtaaag ctactgaaaa agaatggcga
     1981 gaaaacgatg gtttagtatc cgttatttct tctcaacatc catttaatca agcttataca
     2041 aaagcgacag ataaaattca aaaaggcatt tggcaagtga cgcctacaaa acatgattgg
     2101 gatcatgttg actttgtagg acaagacagt tctgatacag tgcgcacaag agaagaatta
     2161 caagattttt ggcatcattt agcagacgat ttagtgaaaa ctgaaaagct gactgatact
     2221 aagcaagcat aatttataaa gtaaagggag gaattaataa tgactgcaga cttctttcaa
     2281 ttaatcggat cattatttag aattctaaaa gaattattca agtaaaacat tggcgaggcc
     2341 ccaacataaa gaatttcgaa aagaaattct acaaacaatg caagttggcg gggccccaac
     2401 aaagaagctg gcggaaagtc agcttacaat aatgtgcaag ttggcggggc cccaacatag
     2461 aagctggcgg aaagtcaggt tacaataatg tgcaagttgg ggtgggacga cgaaataaat
     2521 tttgcgaaaa tatcatttct gtcccactcc cattggcatt tacgaagttt aaatgtgcaa
     2581 ttagaatata tgtataacaa tattaaacac gcggtaaaac gaagtcagtc aattcaaact
     2641 gatttcgccc accgcgtgtt tttaacatag cttaataatt aataagcatt aatgttcaat
     2701 ttatatggtt gttttccaat aataaaccta aagatataga attcacgcaa tatcatgccg
     2761 acacctatac ataatcctaa aatgaatagt agtgatatcg ctagaaagac cattgtatta
     2821 tcctcaaata tatttgtata tgc
//
LOCUS       DANJFZ010000190         2822 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.196, whole genome shotgun sequence.
ACCESSION   DANJFZ010000190 DANJFZ010000000
VERSION     DANJFZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.196"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..1236
                     /locus_tag="PWN45_002724"
     CDS             <1..1236
                     /locus_tag="PWN45_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL0624418.1"
                     /translation="ILKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTS
                     EDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTK
                     KGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQY
                     EIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDAT
                     FTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIIN
                     EIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPS
                     AFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGV
                     AHLPGHLSPLLKKLLLKSL"
     gene            1461..2804
                     /gene="brnQ"
                     /locus_tag="PWN45_002725"
     CDS             1461..2804
                     /gene="brnQ"
                     /locus_tag="PWN45_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0624419.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          977 a          404 c          491 g          950 t
ORIGIN      
        1 attttaaaag aagatatgct aaacgaatat tattacttac ctaaaacact atataataca
       61 ttagctagtc cagagtttga tgatttaaag agaactgatg caagtcaagt cgatgggcaa
      121 gatgatactt cagaagatga tgataacgaa agtgaaaaag ctgactctaa atctgcagat
      181 agtgagtcaa aaggtggcgc ctatttagaa atggagcttc acgaaggaca aaatagtgaa
      241 acattaggta atgatgaagc acgtgaaggc gatgctacag atgatatgac tgatatgatg
      301 accaaaaagg gcaaaggatc aaacgatacg ctaaatcgtg aagaaggcga tgctgttggt
      361 caaagccaag catttcaact tgatggtgtg aacaaaaatg tagaaataaa atggcaaatt
      421 cctgaaattg agccacaata tgttttagaa tatcaagaat cgaaacaaga cgtacaatat
      481 gaaatcaaag acttaattca aattataaag aaaacgattg aacgagaaca acgagatgca
      541 cgttttaatt taacaaaagg gcgattacaa aaggatttaa ttaattggtt tatcgacgat
      601 caatataaat tgttttacaa aaaacaagat ttaagtaaat catttgatgc gacatttaca
      661 ttgttaattg atgcgtcagc aagtatgcat gataaaatgg ctgaaacgaa gaaaggtgta
      721 gtgttattcc acgagacgtt aaaagcattg aacattaaac atgaaatttt atcattcagt
      781 gaggatgcct ttgattcaga cgagcatgca caaccaaata tcattaatga aattattaac
      841 tatgattact caacctttga aaaagatggc ccacgtatta tggcacttga acctcaagat
      901 gataatcgtg atggtgttgc tattagagtt gcaagtgaac gattaatgcg tcgaaatcaa
      961 catcaacgat ttttaatcgt attttcagac ggcgaaccgt ctgcatttaa ttatagtcaa
     1021 gatggtatta ttgatacgta tgaagctgta gaaatgtcac gtaaatttgg tattgaagta
     1081 tttaatgtat ttttgagtca agatccaata actgaagatg ttgaacaaac aattcataat
     1141 atttatggtc aatatgctat tttcgttgaa ggtgttgcgc atttacctgg acatctctct
     1201 ccattattaa aaaaattact acttaaatct ttataaataa atttaaaata tgagtttata
     1261 tgaaattgtg aataaaagtc atcagttact atttaaaaag tggatttgaa gaattttatt
     1321 ttctgacata atttgattta aacaacacat gtgttttgtt tctgctatgt acaacatatt
     1381 tcatatagac tctttattat tttttgtgct aaaatattta cttgtaaatt atttcgtgtt
     1441 ttaaaaaaag gagtaactct atgaataaaa atacatgggt cattgggttt acgctcttcg
     1501 ctatgttttt tggcgcaggc aatctaattt ttcctcctaa tctcggattg gatagtggtc
     1561 aatttttctg gcccgccatt ttagcgtttg ttctaactgg gattggttta ccattattag
     1621 gtgtgattgt aggtgcactt gataaagaag gatatattgg cgcattaaat aaaatttcac
     1681 ctaaattttc aatattgttc ttaatcatca tttatttgac tataggacca ctttttgcaa
     1741 tacctagaac tgcatctaca tcttttgaaa tgacaattac accaattata catagcaata
     1801 gtagtatcgc tttatttata tttacgatta tctacttcat agtcgttttg tatatttgtt
     1861 taaatccatc taagttaatc gatcgtattg gttcattatt aacaccatta ttattgatta
     1921 ctattttagc gatgattatt aaaggatact tagactttag cggtaatagt gctggaaagg
     1981 gcaatgaagc actatatcat tctaattttt caagttttgc tgaaggcttt acacaaggct
     2041 atttaacaat ggatgccatt gcagcaattg ctttttcaat gattgttgtt aatgcagtaa
     2101 aactaacagg cattactaaa acaaatcaaa tattcaaaca aactttgact gctggtttaa
     2161 ttgcagccgt agctttaatt ttcatatata tttcattagg ttatattggt aatcatatgc
     2221 cagtaagtga catgacgtta gatcaattga aatccaaaga tcgaaacatt gggacatatt
     2281 tattaacgac aatggcttca acaggatttg gttcattcgg aaaatattta ttgggcatca
     2341 ttgtggcgct ggcatgtcta actacagcat gcgggcttat tgttgcagtt tctgaatatt
     2401 tccatagaat cgtacctaaa gtatcataca aagcatttgt attagttttc attttaatga
     2461 gttttattat tgctaaccaa ggtttaaatg ctgttatctc aatgtcaatt ccggtattaa
     2521 gcattgtata cccagtagca ataactgttg tattattaat tttaattgcc aaattcatac
     2581 cgacaaaacg catttcacaa caaattccag ttattatcgt atttatattg tcgattttca
     2641 gtgttattag taagttaggt tggctgaaaa ttaactttat agaatcattg cctctaagag
     2701 cgtattcttt agagtggttc ccagtagcaa ttattgcaac gatattaggc tatctagtcg
     2761 gcatatttgt aaaacaagat ccaattaaat atcaacagga ataacgaata atataaaaga
     2821 gg
//
LOCUS       DANJFZ010000191         2790 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.197, whole genome shotgun sequence.
ACCESSION   DANJFZ010000191 DANJFZ010000000
VERSION     DANJFZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.197"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            104..1072
                     /gene="dhaK"
                     /locus_tag="PWN45_002726"
     CDS             104..1072
                     /gene="dhaK"
                     /locus_tag="PWN45_002726"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL0624420.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            1114..1698
                     /gene="dhaL"
                     /locus_tag="PWN45_002727"
     CDS             1114..1698
                     /gene="dhaL"
                     /locus_tag="PWN45_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL0624421.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            1691..2053
                     /locus_tag="PWN45_002728"
     CDS             1691..2053
                     /locus_tag="PWN45_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL0624422.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            2169..2666
                     /locus_tag="PWN45_002729"
     CDS             2169..2666
                     /locus_tag="PWN45_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624423.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
BASE COUNT          978 a          363 c          574 g          875 t
ORIGIN      
        1 ttatcaaaaa tatatttgat aacggtatca ggttaattct ttatgatagc gcattcattt
       61 attctgtttt atactatgac tgataatacc aaggaggtac aacatgatga aaaagttaat
      121 caataaaaaa gaaacatttt taactgatat gcttgaagga ttgttaattg cgcacccaga
      181 gttagatctg attgctaata cagttattgt aaaaaaagct aagaaagaac atggtgtagc
      241 aatagtctct ggaggtggaa gcggacatga acctgcgcat gccggttttg ttgcagaagg
      301 tatgctagat gcagcggttt gtggcgaagt atttacatca cctacacctg ataaaatatt
      361 agaagctatt aaagcagtag atactggtga tggtgtatta ctagttgtaa aaaactatgc
      421 aggtgacgtg atgaatttcg aaatggcaca agagcttgca gaaatggaag gtataaatgt
      481 tcaaactgtt attgttcgtg acgacattgc tgtgacaaac gaagtacaac gtcgtggtgt
      541 tgcaggaaca gtgtttgttc ataagcttgc cggttatctt gctgaaaaag gttattcatt
      601 aacagagata aaatcgcgtg tagaagcgtt gttacctgaa attaaaagta ttggtatggc
      661 aattgagcca ccgcttgttc caactactgg aaaatatggc tttgatattg aagacgacaa
      721 aatggaaatc ggtattggta tacatggtga aaaaggtatt catagggaag aagtaaagga
      781 tattgatcat attgttggaa cattgttaga cgaattgtat aaagaagtta ctgccaatga
      841 tgtcatatta atggtaaatg gtatgggtgg tacgccgtta tctgaattaa atatcgtaac
      901 taaatatatt caacaaaatt tagctgcaag aacggttaat gttgctaaat ggtttgttgg
      961 tgattatatg acatctttag acatgcaagg tttttctata actatcgtgc ctaataaacc
     1021 agaatatttg gaagcatttt tagcaccaac aacaagtcaa tactttaaat aagaagtgaa
     1081 tatgaataaa tatacattta tgaggtggca caaatgaaag tgaatgatat gaaagcacgt
     1141 ttattaaatt tagaagaaac gtttaaaaaa catgaatctg aattaactga attagatcga
     1201 gcaattggtg atggtgacca cggggttaac atggttcgtg ggtttagtag tcttaaagac
     1261 aaacttgatg atagctcaat gcaatcattg ttcaaatcaa ctggtatggc attgatgtca
     1321 aatgttgggg gtgcatcagg accactgtat ggctttagct ttgttaaaat gtctgcagtc
     1381 accaaagatg atatggataa tcaagatttc attacactaa ttcaggcatt tgccgaagcg
     1441 gttgaatcac gtggtaaagt tactttaaat gaaaagacaa tgtatgatgt agtagcgcga
     1501 gcagcagaga agcttaaaaa tggtgaaact ttaacattca atgatttaca gcaattagca
     1561 gataatacaa aagatatggt agcaacgaaa ggtagagctg catattttgg agaagaatca
     1621 aaaggttata ttgatccagg tgctcaaagt atggtttata ttttaaacgc tttgattgga
     1681 gatgaagata atgcctaaaa ttatacttgt tagccacagt aaagaaattg caagtggtac
     1741 aaaatctttg ttaaagcaaa tggcaggtga cgttgatatt ataccaatcg ggggattacc
     1801 agatggttca attggaactt catttgatat catccaagaa gttttgacta aattagagga
     1861 tgatgcattg tgtttttacg atattggatc ttcagaaatg aatgtagata tggcaattga
     1921 aatgtatgat ggtaatcatc gtgtgttaaa agttgatgca ccaattgttg aaggcagttt
     1981 tatcgcagca gtaaagctat caatcggcgg ttcaattgat gatgcattag cagaaatcaa
     2041 acaatcattt tagttaaaat ttactaataa tgaaaaatgt aaaccttttt caaatgaaac
     2101 tttataaaaa atatgatagt atatatgtaa atgtttaata aaatctggag aaataggagg
     2161 acattgccat gcaacacctt ataaaaaaac atgtattgaa tggcgagttt gatttagtac
     2221 gacaattgat gtccgaaaca gattttatgg aatttgaaga agcatatatt tcaagtgcgc
     2281 atgaagtaga aagtatgatg ttttatacat gtattttaga tatgattaag tacgaagaat
     2341 catctgaaat gcatgactta gcatttttat tgcttgtgta tccactaagt gaatatgaag
     2401 gtgctttgga ttctgcttat tatcatgcag acgcttccat aaaacttact gacggcaaag
     2461 aagttaaaag tttgttacaa atgttattat tgcatgcgat accaacacct gttatttcag
     2521 ataagaaggc ttttgatatc gccaagcaaa ttttaaaatt agatcctaat aataatgttg
     2581 ctcgtaacgt cttaaaagac actgccaaac gtatggacaa cgttgttgtt gatataaatg
     2641 aattacacca acgtaatgca cgttaattac atttcaatta tattagctta ataatagttt
     2701 taacatttgg ttgggttggg catatgttcc agcctttttt aatacttaaa aactaacgaa
     2761 gtatacttgt gtgcacaaat ggtttttata
//
LOCUS       DANJFZ010000192         2747 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.198, whole genome shotgun sequence.
ACCESSION   DANJFZ010000192 DANJFZ010000000
VERSION     DANJFZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.198"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            95..2200
                     /gene="rsp"
                     /locus_tag="PWN45_002730"
     CDS             95..2200
                     /gene="rsp"
                     /locus_tag="PWN45_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDL0624424.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            2271..2693
                     /locus_tag="PWN45_002731"
     CDS             2271..2693
                     /locus_tag="PWN45_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL0624425.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
BASE COUNT         1033 a          416 c          376 g          922 t
ORIGIN      
        1 aactataata ggtatactat ccatgatttt aaaaatttcc atgttttaca tttaaatgat
       61 agatttcaat atatattagg caggagagag aaatatgaca tgccaactta aaatacatca
      121 tacattatcg acaataccaa gtcgtaatat taataatatt atggtgctat tttcacttac
      181 aagtaaactc aatattacga ttaatggtga aaccaaagac gtaagtaatt atattatctt
      241 aattaaccat ggtgatattt ataatattaa ccacggcgaa aatataatcg aattaatgat
      301 acccgttttt tatttttatc aacaagatga tgatttcttt aatggttatt tagatcgtca
      361 tttattacaa tcaagtaatt atattaagtc attaattgcc gatttaatca gtacaccaac
      421 aagttcttct ttaatgggga aaaatatagg tcaaagtatt atagacactt tacttaaaga
      481 agcttttata agaatagatc atgaatattt accaaatatt gctttaagca atccggtgtt
      541 catagactgt gtaaactata ttcatgacaa tattgatgcg cacttgtctt taaaagatat
      601 agcgatgcat tgcaacatct ctgaatcata ttgttctaat ctttttgttc gatatttgag
      661 tatgaatttt aaagattatt ttacaagtat taaacttgtt aacgccatca atttattact
      721 ttctacaaaa cattctatta caactgtttc tgaattggcc ggttttaaca gtcatacaaa
      781 ttttgcaaat caatttaaga attacttgca ttttagtcct aaacagttcc gttcactcgt
      841 ttccaagatt actgaaccac cacaaataca cttccaacaa gataacgtat cacaattcac
      901 tgagttaatt tcaacaattg atttaacagc tcaactggct acaaatacaa cagatattca
      961 tatagatgat tttaatccga aagatcgaag tcagcgtgcc aaagtatttg ttcgatttag
     1021 taatttcaat gaactatttc aatttatttt caatgaatat tacgatatta actttgagca
     1081 cttaccaaaa cctgtcgttt tcattgacga tattcacgat attgagatta gccaaacgaa
     1141 ttacaacctg ttaaatagat gttttgaaaa gcttttcgaa aaaaatatag gtttagcaat
     1201 cgctattaaa tcaacacaac aattcgaaac gatgaagcaa ttaatactaa catttttaca
     1261 aggcaatcaa gactataaga caagcaagaa attagttaaa tttatgctcg tattttgctc
     1321 taattctatg acagcagaag aaattcattt atgtcatctt aaaattaaaa acaaaaataa
     1381 agaaattaaa tatagtgtga cagtcgatgg ctttttagaa acttattcaa ccgtggaaca
     1441 agtttatgat gtcatgcagc gtcttaaatt ccattattac tttattgata ttgaaaattc
     1501 aaaaactgca acacatctta tcactaaaaa ccaacactat catcaaactg acactcattt
     1561 tgaacagtat aagaaattta ttttagattc aggtatatca tcaacacaat ttgtatataa
     1621 taacctgtct gtaagcggat ttaaatatac taatgatggt aagaatccaa ttcaattatc
     1681 tgacatagtg tatcacttaa tcgcattatt acgatatggc ggtggcatta gctatcaatt
     1741 attagatgac cattcaaatt atatttcctt gtacaacaaa tatggtagcc ccctaccatt
     1801 aatgcattta tataaaatgt ttagaccttt tgttaatgaa gatattgaaa ttacaaataa
     1861 ttatgtattg agtcgtaaag ataataacta ccatttctta ttattcaata aaattaatga
     1921 tcgatatatg tcagacgtaa aacaagattt cattttccat aatgaattac ctcaagactc
     1981 tttgatgatt attaaaacat tgaatcatga acatggttca attcaacatt tgcttccaat
     2041 aagcgatcaa cttgtttata tagaaaaaga aattttagat gaattagaca aaacgaatta
     2101 ccctaaaacg gagcttgcag ttcaagaaga aactggtcgt acatttgaac tcaagttaaa
     2161 tcacgacgag gttaaatata tttgctttaa accaagctaa atactaacag tcctcttgtg
     2221 tttagtttct tacgttaaag gctatttata tcataaggag atgatttgta atgagtaact
     2281 cacaagcaat tcaagcaatt gaaaacgtgt tagcaacgtc aaaagttggt gtattatcaa
     2341 ctgcatataa taataaacct aatagtagat atatggtctt ttataatgat ggtcttactt
     2401 tatatactaa aacgaatatc cattctgcta aggtcaaaga aattaaagat aatccagcag
     2461 catatgtttt gttaggctat aacgacacaa ctaatcgtag tttcgttgag atggaagcga
     2521 caatcgaaat cgttacagaa caagaagtga ttgattggct atgggaaaca caagacaaaa
     2581 gctttttcag ttcaaaagaa gatccagagt tatgtgtttt aagagtagtt ccgcaatcca
     2641 ttaagctaat gaatgataaa tcattagata cacctatcaa aatcgattta taacacaaag
     2701 tgtatatagg aaataacttt tatgaattct agatataaca atgttaa
//
LOCUS       DANJFZ010000193         2741 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.199, whole genome shotgun sequence.
ACCESSION   DANJFZ010000193 DANJFZ010000000
VERSION     DANJFZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.199"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(218..1261)
                     /locus_tag="PWN45_002732"
     CDS             complement(218..1261)
                     /locus_tag="PWN45_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0624426.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            complement(1412..2479)
                     /locus_tag="PWN45_002733"
     CDS             complement(1412..2479)
                     /locus_tag="PWN45_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624427.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
BASE COUNT          933 a          462 c          385 g          961 t
ORIGIN      
        1 gacaatttag ttgtaaaaag ttattttcag gttcaaaaca aattagtttg taaaataaat
       61 gtcacatcat aatagagtga gatagaaatg ataaagaacc gctaacaatt tattatatct
      121 cagactctta cacattagca ccacatgttt taaattcttc gtttttaatt tgaaaatctt
      181 aaatgttgct taagcaataa attactactg tttatcatta gtttaaagga tttaacggta
      241 attcattact cggattttct atttgtggtt tctcttcagt ttgaatacca ctagaggatg
      301 tattacgact taagaaaagt aacacttttt tgatattatc tatcgattca ggtttattaa
      361 ggtgaaattg atactgatga tgtaaatgat gcgtaccatc ataaaacaaa gtatctactg
      421 gtacattcaa ttcttgtaat ttcttactga attctatatt ttgactttcg aatggatcgc
      481 tatctccaac agataaaaat gttggtggat aattttttgt cgattgtttt actgtcgaca
      541 tttgtgaaat gtttttaaaa ctcttttccc aatcttcttc gccagtataa cttttcataa
      601 ataactgtat tcttggaaac tcagtttctc taactgtttg catattataa aaaccaccaa
      661 atagtatagc acctttaatt tgcgatggtt tgaatgactg atcaaatttc atggcttctc
      721 ttaagcgatc attcgtctgt attgccgtaa attggctagc taattgagca cctgcagaat
      781 caccgccaat aattacttga ttaaaatcaa taggtaaatt cattttattt tctttaatga
      841 attgagttgc ttgattcatt tgaattaatg gtgtgggata tttatattgt ggcgccaatg
      901 cataatttac attcacaaca atgtaccctt gttcagcaat tttcgctaat aatgggtttt
      961 tatactgctt atcacccgca atataaccac caccgtgcat ccaaaaaata actggcagtt
     1021 tggcattaga agacatatca acaggtgtaa taatatctaa tttactgtta gggaaaactc
     1081 tattgtatgt aaggttttga taaatgcgca catttttatt tttaatatta attttgtctt
     1141 tattagcatg gttctcattt gtaatttggt cataatgttg tttcagatga aatgctaaca
     1201 atacactgat tacagttata aaaagcgcag ttatcatcgt ccatttatta tctttattca
     1261 ttacatcgta cctctctatt tgttagacgc tcatattaat agactgaata aatataaaaa
     1321 gcaattcgcc cgataagagt agagacgaat tgcttttaag gttaaaacaa agtgattcaa
     1381 ttgtaaagtt tcggatatcc ctcatatgat attaagcaat atgtttttta tccgcatttt
     1441 tacgtttgaa atatttaata attacagcta ctacaataaa taagcctaca tatcccataa
     1501 ttgtatataa cttattaatt aattcagcaa atcctacgaa acttaataaa taacctgcta
     1561 ccatcattat aataataaag atatgataat ttttactgta tggttctgta aaacgtgctg
     1621 caaatgaata cattagtcct agaatagtat tatacataac cgccagcata ataacagata
     1681 acactgttgc tattaaagga tggatattat ttgctaacgt caatgtagga attgaggcat
     1741 ttttaatagt tggatattca ctttgcaatg caaagttgat caatgctagt aatacagtat
     1801 agataatacc accatacatc gcgcctgcac ctgacactgt acgcttttca gtatccccac
     1861 cgattgctac gatggtacta aaacctacag aaaaagctaa tccaccatat attgtaccaa
     1921 accagattcc cttccaaata cttgcttcag gcactacttg gttaacttct gctaatgaaa
     1981 catgaccttt aaataaataa acgccagcga ttaatacaac taaaacaatt aaaaatggtg
     2041 taacgatacc tagtgcacgt actattttat taaagtctaa tagcaacgta atataaatcg
     2101 ctaatgtcat aattaaagca cctagccatg taggtatgtt ataactttct tcaaatgttg
     2161 cgcctgcacc agctatcatg gttacagcta taccaaataa gaagaagatt aaaatataat
     2221 caaagacttt actaaacttt tcaccgaata cgtattgtaa tgttgattca tgatttgtcg
     2281 cttcaaaggc agtaccaatt tttgctactt ggcgcccaat aaaagctaaa ataaaaccag
     2341 atataataac acctaaataa gcccacaagc catatttagt gaaaaattgc ataacttctt
     2401 gtccagttga aaatcctgcg cccactacaa tgccgacgta ggcaaatcca atttttgttg
     2461 cttctttatt catcatcatg tgaataatag acctccttaa ttttgcacag tgtgcattat
     2521 aacgtactga aaattaattg caagccgcat aggttatttt tcactttttc aaaattgctc
     2581 aatccattca cattatcacg ttcaatgaca tttaaaaaaa ttataaattt atgcttttat
     2641 aatgcagtca tacgaacttt tgtataggaa aaatgattaa ttattgtgct tcttgaattt
     2701 cgtatattta gttaaatttc gaagaataca tatcaaaatt t
//
LOCUS       DANJFZ010000194         2721 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.200, whole genome shotgun sequence.
ACCESSION   DANJFZ010000194 DANJFZ010000000
VERSION     DANJFZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.200"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..562)
                     /locus_tag="PWN45_002734"
     CDS             complement(<1..562)
                     /locus_tag="PWN45_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624428.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVN"
     gene            complement(576..1367)
                     /locus_tag="PWN45_002735"
     CDS             complement(576..1367)
                     /locus_tag="PWN45_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL0624429.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(1548..1751)
                     /locus_tag="PWN45_002736"
     CDS             complement(1548..1751)
                     /locus_tag="PWN45_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624430.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(1780..2589)
                     /locus_tag="PWN45_002737"
     CDS             complement(1780..2589)
                     /locus_tag="PWN45_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0624431.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          852 a          478 c          369 g         1022 t
ORIGIN      
        1 tatttactaa aatatcatct aaatctgaat gtatagatgg tatatcaaag aaatcttggc
       61 ttaaaaatgc atgttctaaa tacaaaaata ataaatctgt atattgtgtt ttagtacgat
      121 acactttaat ttgagattcc gtatatgata tacgtgtatc taagcgaaga tcaattaatt
      181 tagcagtact tgggcgctca actttaatag aatttaatac gcgcatatct tctaataatt
      241 taaaaagttg ttgataaaat ttagggtgtt taaaagtttt atcttgtact acttcattta
      301 caatttgtac atccatcata tgataaccgt aagcagctaa cataacatct gtttttaaac
      361 cagccatttc gatatggctt ggtcgatgtg accaaaacca actcgtaatc aaaacatttt
      421 gaacaggatt atagtaaggg aatttttgga ttttaacttg tgtttgttca ttttttaata
      481 agagtcgagc taagtcttgc aacatcatta cttgcttggc atctaactgt tcgtcattaa
      541 attttatgaa acgatcactc atgttttatc cctctttaaa agtttagttc tacagcatta
      601 tatattgctt gttgttcacg ttcatcttcc aatttatcaa taattgtacg tttaattgca
      661 cgttcaactg gcattacagt gattaaatca cacaagtcta ataatgcacg gatactagcg
      721 gcttcttcag aaatttgtcc ctgcttagac atagtacgta aatcttcatt aaacttaata
      781 atttgttcga tttgtttatc accttgtaat aaactttgct ctttaatcac attttttaaa
      841 atgtccccat caatataatc aacgtgaata acaacaaagc gattttttag tgcttcattc
      901 attggcaaag taccaacata accttcattt atcgctgcta taacgttaaa tcctggtaca
      961 gctttgatta cttcaccagt gtatggattc gtaatttgac gacgataatc taagacccca
     1021 tttaatacag gcaatgtttc aggtttagcc atatttattt catcaatata taaaatatgc
     1081 ccctctttca tagctttaat aactggacca tctacaaaga caatttcttg ttgaccttcc
     1141 gcatttgttt taattgtttt aaagcctaat aagctttctg tatctaaatc aacagaacaa
     1201 ttgacttgat gcatgggtgt atcaacaact tcacttaatg tttctgccaa ctttgttttc
     1261 cctgaacctg ttggaccttt aagtaaaata tttttattta aatcaaataa tgccttcgca
     1321 tcattgaaaa ctgttgaatc tgaattctta taatgtttta gtgccatttt aaaacgttct
     1381 cctttaaata aattaaaaaa ctaagacatg gaataagttc tccaaatgcc ttagtttact
     1441 attctattct gatataaaac gcaatgctta gtatgcgaat tgtttatatt ttaccaaatt
     1501 tactaattga aaatctcata ttaggcttca tttcaataat tatgcgttta aacttctttg
     1561 aaatatgttt tatagtaacc gccaactaat ccagaattat ctattatttg ataatattct
     1621 tcagtttcgt tgataacatc atattgtttg ccaaccgtta acatgtcaga aactgtaaat
     1681 ttctttgcat ctgtattaat gacttctact ttcttgattg gtgtacgttc tttccaagtt
     1741 tcatgtaaca taaattttgt cctcgctatc gtaaaaaaat tatttttgta aagtgaatat
     1801 aaacgataat ccactgtttt cagtaattct aattgattca atatcatctt gctcaagcgt
     1861 ttgatatggt atttccactt catctaagcg cgatttcgct tgattaaaga aatctgcatc
     1921 tttcgtttca aactcaactt gatcgactgc gccatattcc ggcattgtca tttcggtttc
     1981 tacaggcatt aaatgtattt cgccacctag accacccgtt ccaactttga atacttggac
     2041 atgatagtca gcattgtcga agggttggta ttctgcaaat acttcaagtc cgaatatatt
     2101 tgttaaaatt tgacctgtaa tatctacatg attcacttta agaatcactg gtcctaaacc
     2161 ttgcacttga tgtaacggat tgaccgcact ctcaaaagaa ggcataccta aaccaacgcc
     2221 ataattattc tcgtttgaat atattgagaa aatatgacca ttgttatctt cgagactgaa
     2281 atatttattg ccatttaatt ctttaactgt tgtaaatgga atatccttat tcgataatat
     2341 ttccgcatac tcctctaaac ctgagtcagt tggtgtacgt aatccaatac ccacaaagtg
     2401 agattcttct aattgctcac ttggaacttg tataaattga agtcttgttc ctgggcttat
     2461 atctgcatcg ccgaaacgaa ttgagttttc aagaagttct tcataattta atcctaatat
     2521 gtcaaccatg aaatgttttg tctgttctat atttgttgta cctaatgtta tacttctaag
     2581 tccacacatt atagccacct ctatctttat ctttactatt atattataac ttattttaat
     2641 cataaattat agaatatcgg tcagttgatt ttgattatag attaatatgt gaaaaatgta
     2701 tttctttaaa agaactattt c
//
LOCUS       DANJFZ010000195         2681 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.201, whole genome shotgun sequence.
ACCESSION   DANJFZ010000195 DANJFZ010000000
VERSION     DANJFZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.201"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..526
                     /locus_tag="PWN45_002738"
     CDS             <1..526
                     /locus_tag="PWN45_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL0624432.1"
                     /translation="PKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGGNDHTLV
                     TESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEALKYIDYD
                     LDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKGPFAPDF
                     IKVWKERYKKIRQY"
     gene            817..2553
                     /locus_tag="PWN45_002739"
     CDS             817..2553
                     /locus_tag="PWN45_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDL0624433.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            2562..2654
                     /locus_tag="PWN45_002740"
     CDS             2562..2654
                     /locus_tag="PWN45_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624434.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
BASE COUNT          946 a          356 c          505 g          874 t
ORIGIN      
        1 acctaaagaa ggggaaaaca ttaaaattca gagttataaa catgatggta agattcatcg
       61 cgtttggtct gaaacaacaa tattaaaggg tactgatcat gttgtcattg gtggaaatga
      121 tcatacgtta gtgactgaaa gtgacggaag aacttggata acacgtgaac cggcaattgt
      181 ttactttcac tcagaatatt ggtttaatgt tatctgcatg tttagagaag atggaattta
      241 ttactattgc aatttgtcat caccatttgt ttgtgatgaa gaggcattaa aatatataga
      301 ctatgattta gatattaaag tatatccaaa cggaaagtat catttattag atgaagatga
      361 atatgagcaa catatgaatc aaatgaatta tccgcatgat attgatatta ttttaagacg
      421 caacgtggac attcttcaac aatggataga acagaagaag ggcccattcg cgccagattt
      481 tatcaaagtt tggaaagaac gttacaaaaa aattagacag tattaagcaa ataaagtgta
      541 aatactgatt aaatcattta tattgactta gtgatgtaaa gaagtatgat aacttttgct
      601 gtcgtgtggg gacacagtat ccatagatta aattgaatag aatataacat acgaataaga
      661 caacgtattt aatcacatga atatgacaga ggttgagata tcttaatttt aaaaatgtaa
      721 attgtaagaa gaatttacat ctacaaatta tgtatccaac ctctattcat ttttatgctt
      781 atgaatagga tagggcaata gattaggggg gattgtatga ttaaacgata tttgcaattt
      841 gttaagccat acaaatatcg tatttttgca acgattattg ttgggataat taagtttggt
      901 ataccaatgc ttataccact attaattaaa tatgcaatag atggcgtgat taataaccac
      961 gcactaacga ctgatgaaaa agttcatcat ttaactattg ccattggtat cgcattattt
     1021 atttttgtaa tagtgagacc accaattgaa tttatacgtc aatatttggc gcaatggaca
     1081 agtaataaaa tattgtatga tatacgtaaa aagttataca accatttaca agctttaagt
     1141 gcgagatttt atgctaataa tcaagtaggt caagtaatat ctagagtgat taatgatgtt
     1201 gaacaaacaa aagatttcat tttaaccggg ttaatgaata tttggttaga ttgtataaca
     1261 attattattg cactatccat aatgttcttt ttagatgtga aattgacttt agcagcactg
     1321 tttatcttcc cattttacat tttaacggtg tacgttttct ttggaagatt aagaaaattg
     1381 acacgtgaaa gatctcaagc attagctgag gttcaaggat tcttgcatga acgtgttcaa
     1441 ggtatttcag tcgttaaaag ttttgcgatt gaagacaatg aagcgaaaaa ctttgataaa
     1501 aagaatacta atttcctaac acgtgcgttg aaacatacaa gatggaatgc ctattccttt
     1561 gccgcaatta atacagttac agatattgga ccaattattg tcatcggtgt tggtgcatat
     1621 cttgctattt ctggatcaat cacagtaggt acacttgcag catttgttgg atacttagag
     1681 ttattgttcg ggcctttacg tcgtttagtc gcatcattta caactttaac gcaaagtttt
     1741 gcttcaatgg accgtgtatt ccaattaatt gatgaagatt atgacatcaa aaatggtgtt
     1801 ggtgctcaac ctattgaaat taaacaaggt cgtattgata ttgatcatgt tagttttcaa
     1861 tataacgata acgaagctcc aattttaaaa gatattaatt tgagtattga aaaaggagaa
     1921 acagttgctt tcgtaggtat gagtggtggt ggtaaatcaa cattaattaa cttaataccg
     1981 agattttacg atgtaacttc tgggcaaatt ttaatagatg gtcacaacat taaagatttt
     2041 ttaacgggaa gtttaagaaa tcaaatagga ttggtgcaac aggataatat tttattctct
     2101 gatacagtta aggaaaatat tttacttggt cgtccaacag caacagatga agaagtagtt
     2161 gaagcggcga aaatggctaa tgcacatgac tttattatga acttgccaca gggatatgac
     2221 actgaagtag gtgaacgagg tgttaaatta tcaggtggtc aaaaacaaag attatcgatt
     2281 gctagaatat ttttaaataa tccgccaatt cttatcttgg atgaagcaac aagtgcactt
     2341 gatttagaaa gtgaatccat tattcaagaa gcattagatg tgttgagtaa agatcgaacg
     2401 acacttatcg tagcgcatcg cttgtccact attacacatg ctgacaaaat tgtcgtaatt
     2461 gaaaatggac atattgttga aacaggtacg catcgtgaat tgattgcaaa acaaggtgct
     2521 tacgagcatt tatatagcat tcaaaactta taaagtatta gttgtttgac ttcagtacaa
     2581 tcttgaagag aaaatttgta acaggatggt ggggtcataa catagaaaaa gcagtaagag
     2641 attttcttaa ttgaaaataa tcttactgct tttttaaatt t
//
LOCUS       DANJFZ010000196         2675 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.202, whole genome shotgun sequence.
ACCESSION   DANJFZ010000196 DANJFZ010000000
VERSION     DANJFZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.202"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            221..1456
                     /gene="arcA"
                     /locus_tag="PWN45_002741"
     CDS             221..1456
                     /gene="arcA"
                     /locus_tag="PWN45_002741"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL0624435.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1542..>2675
                     /locus_tag="PWN45_002742"
     CDS             1542..>2675
                     /locus_tag="PWN45_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0624436.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAF"
BASE COUNT          926 a          374 c          511 g          864 t
ORIGIN      
        1 agtaaaaaat ctttttgctt gaataagata aggttttaac aatgagattt ttcaaattat
       61 tcactattat atagtacaaa atacatattc atacaaaatt ttgcattgaa taaatctata
      121 tatgtgaaaa ccctccctaa acaattgtga attaaatcac acgatgtttt ttgaaagcgt
      181 tttataatgt gaatgtaata aagcaaagga ggaatttgaa atggtacaag gacccattca
      241 agtaaatagt gaaataggca aattgaaaac tgtgttgtta aaaagaccag gaaaagaatt
      301 agaaaattta gtacctgatc atttaagtgg tttattattc gatgatattc cctacttaaa
      361 agttgcacaa gaagagcatg acaaatttgc tcaaactttg agagatgaag gaatcgaagt
      421 agtttattta gaaaaacttg cagcagaatc tattactgag ccagaagtac gcgagaactt
      481 cataaacgac atattaacag aatctaaaaa gacaatatta ggtcatgaaa ctgaaattaa
      541 agaattcttt tcaaagttat ctgaccaaga acttgtaaat aaaatcatgg ctggcgtacg
      601 taaagaagaa attcaacttg aaacaaccca tttagtagaa tatatggatg atagatatcc
      661 attttactta gatccaatgc ccaaccttta ttttacaaga gatccccaag cttcaattgg
      721 tagaggaatg acaattaaca gaatgtattg gagagcacga cgtagagaat ctatttttat
      781 gacatatata ctgaaacatc atccaagatt taaagataaa gatgtaccag tatggttaga
      841 tcgtaactca ccatttaata ttgaaggtgg agatgaatta gtattatcga aagatgtttt
      901 agctattggt atatcagaac gtacatcagc tcaagcaata gaaaagttag cacgtaatat
      961 tttcaaagat gcaaacacaa gttttaaaaa aatcgtagct attgaaatac ctaatacacg
     1021 tacatttatg cacctagata cagtactaac tatgattgac tacgataagt ttacagtaca
     1081 tgcagcaata tttaaagaag aaaataatat gaatatattt accatagaac aaaatgatgg
     1141 taaggacgat ataaaaatta ctcgttctag caagttacgt gaaacacttg ctgaagtttt
     1201 agaagtagaa aaagtggact ttattccaac aggtaatggc gacgttattg atggtgcacg
     1261 tgaacaatgg aatgatggct caaacacatt atgtattcga ccaggggttg tggtgacata
     1321 cgatcgcaac tatgtatcaa accaactttt acgcgacaaa ggaattaaag tgattgaaat
     1381 tactggtagt gaacttgtac gtggacgcgg aggcccaaga tgtatgagtc agccgttatt
     1441 tagagaagat atttaaattt gaaaggtggt aaaagtcaca agtaattcat tcattaaatc
     1501 aaataacaag atttttacca cctttattag gggagattaa tatggatgaa aataaattag
     1561 gtaaaacttc cttaattggt ttagtcatag gctctatgat aggcggtggt gcattcaata
     1621 tcatctcaga tatgggtggc caagctggtg gacttgcaat aattatcggt tggataataa
     1681 ctgctattgg tatgatttct cttgctttcg tatttcaaaa tttaacaaat gagcgaccag
     1741 atcttgatgg aggaatttat agttatgctc aaacagggtt tggagatttt attggttttt
     1801 caagtgcttg gggatattgg tttgcagcat ttctaggtaa tgtggcttat gcaaccctat
     1861 taatgtcagc tgtgggtaac tttttcccta tatttaaagg aggtaacaca cttccaagta
     1921 ttatcatagc atcaatttta ttatggggtg tacatttttt aatacttaga ggtgtagaaa
     1981 ctgcagcgtt tataaatagt attgttacag tagctaaatt aatacctata tttctagtta
     2041 ttatatgcat gatagttgta ttcaacttca gtacttttaa atccggtttt tatggtatga
     2101 ctagtggaag tgttggcgtt tttagttggg gagatacaat ggcacaagta aaaagtacta
     2161 tgttagtaac tgtatgggta ttcacaggga ttgaaggagc cgttgtcttt tctggacgtg
     2221 caaagtctaa aaaggatgta ggaactgcta ccgttattgg tttgatttct gtgctagtca
     2281 tttatttctt aatgactgta ctagcccaag gtgtcattca gcagaaccaa atttcaaaac
     2341 ttgctaatcc atcaatggca caagtattag aacatattgt aggtcattgg ggttcagtgt
     2401 tagttaatat aggcttaatt atctctgttt taggagcttg gttaggatgg acattactag
     2461 ctggtgaatt accattcatt gtagctaaag atggactttt cccgaaatgg tttgctaaag
     2521 aaaataagaa taaagctccg gtcaacgctt taattattac taatatatta gttcagttat
     2581 ttttaattag tatgttgttt acagatagtg cctatcagtt tgcgttttca cttgcatcaa
     2641 gtgcaatctt aattccatat acactcagtg ctttt
//
LOCUS       DANJFZ010000197         2673 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.203, whole genome shotgun sequence.
ACCESSION   DANJFZ010000197 DANJFZ010000000
VERSION     DANJFZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.203"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(196..591)
                     /gene="spxA"
                     /locus_tag="PWN45_002743"
     CDS             complement(196..591)
                     /gene="spxA"
                     /locus_tag="PWN45_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0624437.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            886..1875
                     /gene="trpS"
                     /locus_tag="PWN45_002744"
     CDS             886..1875
                     /gene="trpS"
                     /locus_tag="PWN45_002744"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL0624438.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1917..>2673)
                     /locus_tag="PWN45_002745"
     CDS             complement(1917..>2673)
                     /locus_tag="PWN45_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0624439.1"
                     /translation="APFPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISL
                     SIGITSTIGMLLIGITVGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDK
                     IKNPYGSAIILVLVIIVLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHL
                     LPNILSVVIVQATLLFAGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPW
                     IWMPPAIMITLTILSINFVGEGIKDAFNPRGRR"
BASE COUNT          966 a          436 c          404 g          867 t
ORIGIN      
        1 cttaatttaa ttttacctta tcatagcaaa tgtttcatcc aaaacactct aatatagagc
       61 tatattaaca attgcacatt tgatttaaaa gatactaagc aattattgta aaattaaaac
      121 caaaaaagca acactataga aatagtgttg cttgacatat cttttagtaa agtatgacac
      181 atagttaaat ggttattagt caaccatacg ttgtgcttct tgtaattgga acgtacgaac
      241 ttttctaggt aagaaacgtc gaatctcgtc ctcattataa ccaacttgta gtcgtttatt
      301 atctaaaata attggacgac gtaataagcc aggattatct tgaatgattg aatataagtc
      361 ttgtaatggt agtgaatcaa tatcaacatt taatttttgg tatgttttag aacgtgtaga
      421 aatgatttca tcagtaccgt cttcagtcat ttttaatatt tgcttaattt catcaattgt
      481 taaatgttca gaaaaaatat tacgctccgt atacggaatg tcatgttctt gtaaccatgc
      541 tttcgcttta cggcaagatg tgcaacttgg tgaagtaaat aatgttacca tacatctcac
      601 tctcctattt gaatgaataa aattcattgc ttaaaattta gttatagatc aagaaaaaac
      661 atttttttct aaaattctta atcgttacta tttattataa ctatctaaca ttaaaattaa
      721 atgagaaaaa cctaattttt cagataagtt tctacactta taaaaaagat tattaatccc
      781 tttgttagta gtaagttata cgtatattct aacacatctt acatttttaa gaaatactgt
      841 tataatgata attattaaaa tattactaag aaagtaggca tttaaatgga gacattattt
      901 tcaggcatcc aacctagtgg aattcctact attggaaatt atattggcgc actaaaacaa
      961 tttgttgatg tgcaaaatga ctatgattgt tatttctgta tcgtagatca acatgcaatt
     1021 acaatgccac aagatcgttt aaaattacgt aaacagacca gacaattagc agcgatttat
     1081 ttagcttctg gtatagatcc agacaaagca acattgttca tacaatctga agtccctgca
     1141 cacgtacaag caggatggat gttaactacg attgcttctg ttggagaatt agagcgtatg
     1201 acgcaataca aagataaagc tcagaaagca gttgaaggta tacctgctgg tctattaaca
     1261 tatccacctt taatggcagc tgatattgtt ctttacaata ctaatatcgt tccagttgga
     1321 gatgaccaaa agcagcatat cgaattgact cgtaaccttg tagatagatt taatagtcgc
     1381 tataatgatg tgcttgtgaa acctgaaatt cgtatgccta aagttggtgg acgtgtcatg
     1441 agtttacaag acccaacaag aaaaatgagt aagagtgatg ataatgctaa aaacttcatt
     1501 tcattattag acgagccgaa tgttgcagct aaaaaaatta aaagcgcagt aactgattca
     1561 gatggtatta ttaaatttga tcgtgacaac aagccaggta taacaaattt aatttcaata
     1621 tacgctggat taacagacat gccaattaaa gatattgagg caaaatatga gggcgaaggt
     1681 tatggtaaat ttaaaggtga ccttgctgaa atagttaaag catttttagt agaattccaa
     1741 gaaaaatacg aaagtttcta taactcagat aaacttgatg atattttaga tcaaggtaga
     1801 gataaagcac acaaagtttc atttaaaact gtcaaaaaaa tggaaaaagc gatgggttta
     1861 ggacgtaaga gataaaataa agaggtacta gaattaacta gtgcctcttt tattatttaa
     1921 cgtctacctc taggattaaa agcatcttta atcccttccc ctacaaagtt tatacttaat
     1981 atagttaatg taatcattat agcaggtggc atccatatcc acggtttacc acttataaca
     2041 tccccttctt gagcatcact caacatatta ccccaagatg gtattgcttt actaattccg
     2101 aatcctaaaa agctcaatcc tgattccact acaatcatac cggcaaataa caatgttgct
     2161 tgtacgataa ctacacttaa tatattcggc aaaagatgtt tcaaaataat tttatatgtg
     2221 ggtgtaccaa ttgattttgc tgccaaaaag tattcatttt ctttttcttg aagtacttta
     2281 ccacgaacaa gtcttgcaat acctccccaa cttaatacga taataactag aacaagaatt
     2341 atggcagatc cataaggatt tttaatttta tctccaagtg cagcatttaa tacaattgca
     2401 aatattaaaa atggaaataa cataacaaat tcggttattc tcattaataa tgtatcaaca
     2461 atacctccaa aataaccaga aatcactcca actgtaattc caatcaaaag cattcctatt
     2521 gtagatgtaa ttccaatgga taatgaaata cgacctgcat ataacaaacg actaaagtta
     2581 tccctaccac cagagtctgt accaagaatg ttttgtgctg tcatttcacc ttttatattt
     2641 aataaatctt gttggttcac tggaaaagga gct
//
LOCUS       DANJFZ010000198         2667 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.204, whole genome shotgun sequence.
ACCESSION   DANJFZ010000198 DANJFZ010000000
VERSION     DANJFZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.204"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(189..1325)
                     /locus_tag="PWN45_002746"
     CDS             complement(189..1325)
                     /locus_tag="PWN45_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL0624440.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1357..1986)
                     /locus_tag="PWN45_002747"
     CDS             complement(1357..1986)
                     /locus_tag="PWN45_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL0624441.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(2005..2274)
                     /locus_tag="PWN45_002748"
     CDS             complement(2005..2274)
                     /locus_tag="PWN45_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL0624442.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2437..>2667
                     /locus_tag="PWN45_002749"
     CDS             2437..>2667
                     /locus_tag="PWN45_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL0624443.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCE"
BASE COUNT          805 a          422 c          416 g         1024 t
ORIGIN      
        1 gctaggtttt gtattaatgg cacttaatat aaggaaaata gcagctcaac gagctgtaca
       61 ttataaaata catatcaaaa aagctgattt ctatcaaata aataatagaa atcagctttt
      121 ttacattgcc taagaacttt aaggaactta atgtcccaag ctcttttttg ttatatctaa
      181 ttcgtaattt atgattgttt attcggtcct ttgatgttca ctaaatgtga ctttaaatct
      241 tgttctaatt gttgtaattc tttttcagct aattgtcgtt cttcgcgacc gtgttgttga
      301 ataattaatg tttcttcaat tgtctcaata atgttacgct gtgtacgttt caatgtatca
      361 agatcaacaa tgccacgctc attttctgtt gcagtttcaa tcgcattttg tttcaacatt
      421 tcagcatttg ctgttaataa atcattagtt gtatcagtga cagctcgttg tgcagcaact
      481 gcattacgct gtctcattaa tgtaagcgca atggccattt gatttttcca tagtggaata
      541 cttgtcaaaa ttgaactttg tatcttctcg gcaagtgctt gattaacatt ttgaatcata
      601 cgaatttgtg gcgcagtttg caaagctatt tgtcttgaaa gctgtaagtc atatatgcgt
      661 ttatctagtc tatctataaa ttgctgcata tctgcaactt gttgaatatc catttgatta
      721 gtggattgct gcgcttgctg ttgcaattgt ggtagctttt cattttctaa ttgcaacttt
      781 ttttgctgtg cagcaatgat atgcaatgat aagtcatcaa agtattgttt gtttttatca
      841 tatagcgtat ctaataattc aatatctctt gttaaatgtg tttgatgttt ctgcagttga
      901 atcgttatgc gatcgacttg agcactaact gattgcattc ttgaaaagat ttcattgata
      961 gacgactttg ctctgctaaa aattcttttt aacatagatg gtttatcagt atttaactca
     1021 tttggattaa ctgactttag ttttgacatt agatctgaca aagtatctcc aataggacca
     1081 acatctttac tttgtacttc atccaacatt tgatgtgaaa attgagacat ttgtttctgt
     1141 aaatcagaac caaacgctaa taaaccttca ttgtctaaag ggttaatttg tttactgatt
     1201 gtgtctacct gtttttgttg ttcaattgtt aacttttctt tttgaatagt agtattcgat
     1261 aattctttat cgcttataaa atcatctagt ggatggcttt ctttgaaact tttattttca
     1321 gtcatcaatc atctctccaa tagttgctat tttacatcaa tcttgatgat gtttttgatg
     1381 taacttattt aattccattt caatatctaa acgttcataa tcatcttcgt tgagacgctt
     1441 taaatcagcg attaatgttc gtttgacctc atccaaagta attcgtgttt gttctaactt
     1501 ttgctgttca ttaattgatt ttttgggcat ttttgctaga cgtgtatatg catcaaccaa
     1561 atttaaagca ttatcaatat gagaataaaa aaagccttca actttataaa atgatgcagg
     1621 tctctgtcta actgtcgtat aaatagaacg tgaaatttgg tatatatcat taatctgcct
     1681 aaaatcttta attgatctta tattgacata cgtttttaaa atacctctaa gtttttggtg
     1741 tgtatgattt aactgatttc gaatatagcg atagtctttt ctagtcaaac caatttcgtt
     1801 taaatatttg cgtgaagtga gtttttgtat cggtaggtat gtcattaaaa agccaacaat
     1861 accaatagac atatcaatta aaaaagatac atcaagtgca atcatcccaa atatgcttgt
     1921 taaaaacgct acaggaattc ccactaacac cccaaatata tgagaaatat tatatctcac
     1981 tgtcatcttc ctttatttag cattttatat tgatcgaaaa tccgataatt tttgatttag
     2041 ttctaactct tcaagttgat ggcttgttac atttgatgct ggtgaggcac cttcaattac
     2101 accttgaata aatctctcta tatctgcgtc atccccttgt gcatatatct ctacatagtc
     2161 atctacattt tgaacagtac cgacaatgtt atagttcatt gcaatgcgtt gtgtaaaata
     2221 tctaaatccg acgccttgaa cgcgtccgaa tacttgtaaa tgtatatgtc tcatttttac
     2281 cacctcataa tgttattata cgtagtttta cttaaaaaaa ctaataatta ctatagttac
     2341 tactttgttt gtttcaagtc gtcaaacttg attttcagag gataaaggta taaaaataag
     2401 tatagagttt ttgaagtatg gaaggggtct ttaataatgt ggacagttac caaaattaga
     2461 gccgattatg agggatggtg gttattcagt gactggccag aaaacattgt tgaaaaatat
     2521 caatatcaag attttgatga catgtttaag cactatcaac aattgattaa tcaatgtaaa
     2581 gttcagttcg ataactatgt cacaggcaaa tataatattt atgcatttta taataattgt
     2641 gacatgaact attgcgaaga ttgtgaa
//
LOCUS       DANJFZ010000199         2588 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.205, whole genome shotgun sequence.
ACCESSION   DANJFZ010000199 DANJFZ010000000
VERSION     DANJFZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.205"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            253..921
                     /gene="queC"
                     /locus_tag="PWN45_002750"
     CDS             253..921
                     /gene="queC"
                     /locus_tag="PWN45_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL0624444.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            923..1342
                     /gene="queD"
                     /locus_tag="PWN45_002751"
     CDS             923..1342
                     /gene="queD"
                     /locus_tag="PWN45_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL0624445.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1346..2059
                     /gene="queE"
                     /locus_tag="PWN45_002752"
     CDS             1346..2059
                     /gene="queE"
                     /locus_tag="PWN45_002752"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL0624446.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2158..>2588
                     /locus_tag="PWN45_002753"
     CDS             2158..>2588
                     /locus_tag="PWN45_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624447.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAEN"
BASE COUNT          914 a          415 c          423 g          836 t
ORIGIN      
        1 aatatatggt atattctatt catcgtacat aaatgaatat cagaggttcc tagctgaaac
       61 cctctataaa aaactagaca ttgaaatttc aaacattcgt tggtaaacct ttaatatata
      121 ttataggagc aacccatttc tttcgtctat cttttatgga gataggcttt ttttatttta
      181 taaattaaaa acgggcttat caatgccatt aacctcttta ttaatattat taatgatagg
      241 agcaattaca aaatggaaag tgtattaaat aatgaaaaag ccatcgttgt ctttagtggt
      301 ggtcaagaca gtacaacatg tctcttttat gcaaaaaaac atttcaaaga agttgaactc
      361 gtaacgttta attatggcca aagacatgat actgaaattg aagtcgcaaa acaaattgca
      421 caagatcaag gaatgaaaca tcacgtttta gatatgtcat tattatcaca acttactcca
      481 aacgcattaa cacaacatga tatggaaatt actaataatg aagatggtat acctaataca
      541 tttgttccag ctagaaattt acttttcttg tcgtttgcag gcgctctagc ttatcaaatt
      601 ggggctaagc atattattac aggcgtatgt gaaacagact tttcaggcta cccagactgt
      661 cgcgatagtt ttataaaatc aatgaacgta acattaagcc tagctatgga caaagatttt
      721 gtcattcata ctcctttaat gtggttaaac aaagcagaaa cgtggaaatt aagtgatgaa
      781 ctcgaagttt tagattatat tcgtacaaaa acattaacat gctataacgg tatcattggg
      841 gatggctgtg gtgaatgtcc agcttgtcat ttacgtcaac gtggactaaa tcaatatctt
      901 gaaagtaaag gagcgcttta aaatgttaca acaaatctat cctagtacaa cgcatccata
      961 tcaattcgaa ttaaataaag attttaattt ttcggctgca catcacattc catgtgaaga
     1021 agcaggtatt tgtcaaaatg tccatggtca tacttacttt gttaatttaa caattgtcgg
     1081 tgataaacta gatgacactg gcttcttagt gaactttagc catttgaaaa agatgataca
     1141 cggtaaattt gaccatcaac tgttaaataa cttacctgct tttaaaaaca aaatcccttc
     1201 aactgaaatc gtagcggaaa caatttatca aattgttaaa gaaaatttgg catcgctcga
     1261 acaccaacca aaatgtattc aagtatttgt aagagaaaca ccaacaagtt atgtcgtatt
     1321 tagaccaaag gaacaggtgt aactaatggc taaaattcct gtactcgaaa tattcggtcc
     1381 aaccattcag ggtgaaggtc gagtaatcgg tagaaaaacg atgtttgtga gaacagctgg
     1441 atgtgattat cgctgtagct ggtgtgattc tgcatttact tgggatggta gtgctaaagg
     1501 cgatataaaa ctcatgaccg ctgaagaaat ttatgatgaa ttaaaacgaa ttggtggcga
     1561 tttatttaac cacgttacaa tttctggtgg taacccagca ttaattaaag gtatccaaga
     1621 gttagttgac ttatttcaag ataaaggtat tttcagcgca ctggagacac aaggcagtaa
     1681 attccaacct tggatgacac aaattgatga tttaacgatt agtccaaaac caccaagttc
     1741 aactatgaca cctgatttaa aaaagttaga tgaagtgatt acacaatgcg taccttcatc
     1801 attaaactta aaagtagttg tattcgacga taaagattat gattttgcca aaatgataca
     1861 ccaccgttat ccagatattc ccttctattt acaagttggt aacccatatt tatcagacag
     1921 cgtagataat cataccgaaa agttgttaga acgctacgag cagttggtgg atttagttat
     1981 gcaaagtaat gatatgaatc acgtttatgt gttaccacaa cttcatacat tactttggag
     2041 caataaaaaa ggtgtataaa ggataactta ttttactttt tgcaagtcat atctaatgaa
     2101 taacaatata ttaattgata taatagttga ttgaagtata acgaacagga gtccatcatg
     2161 attgtatata tattaattaa tattgctatc gtcgtactta taactggatt caatttatat
     2221 aggcatcaaa tgcaacattt atcattaagt gcaatgttgt tatctattac gataaacgcc
     2281 tttataaata cattcataat tgataaatat aactttatta ctctatgtac tataacgatg
     2341 tttatcattt ggacgatact acagttttac attgataaaa aattaaaacc tgtatacatt
     2401 acagaccaaa aatttattgc tatcatatta acgattgttg tcagtttaac gcaacgtgtt
     2461 accgactttt caagtacaca atcaatatat atgtctattc catttttagc ccctgccatt
     2521 ttcatcattg gtggtattat gttgtttatt tcaacattta atagcttaga tgaaactgct
     2581 gaaaataa
//
LOCUS       DANJFZ010000200         2576 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.206, whole genome shotgun sequence.
ACCESSION   DANJFZ010000200 DANJFZ010000000
VERSION     DANJFZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.206"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(112..561)
                     /locus_tag="PWN45_002754"
     CDS             complement(112..561)
                     /locus_tag="PWN45_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0624448.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            complement(928..2457)
                     /gene="aur"
                     /locus_tag="PWN45_002755"
     CDS             complement(928..2457)
                     /gene="aur"
                     /locus_tag="PWN45_002755"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDL0624449.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
BASE COUNT          723 a          477 c          352 g         1024 t
ORIGIN      
        1 gactatgaat tctttctttc cataaaaaaa cgcgtacaca agttaatatt tggtaactaa
       61 cttgagtaca cgtgatacga aacaaatttt tattattcat ttgatacatg tctatttaaa
      121 taattttgca tatacgtatt ctgccatatc atttgaggca gttaagatta atgtagtgtc
      181 atttccactc accgtaccta atatctcttc tatatttagc tgatcgataa aatagtttat
      241 gccttgggcg aaaccaggtg aggttttgac gataatgtat gaaccattga tgattgaact
      301 tagaacttca tgttcacaat agtgtctaaa cttttctctc atctcttgtt ctgtttgatt
      361 attaatagct ttataaatcc atgtctcaca atcgataggg acacgatata tatttagttc
      421 cttcaagtct ttagcaattg ttgttgcact atatcttaca ccaaaatatt cttcaatata
      481 tgaaatgatt tgttcttttt tataaatctt atgcttttta actattgtag aaacaatttc
      541 taatcgttta cttttcttca tattgtaaac tcctttggta gttacgtttc ttgtattaaa
      601 aaataaattc atgcatgttt catttataat ttaacacttt gttttgcaaa atataataaa
      661 aatacatgta aattttttgt gacaactttt aaaatgaatt ttgtattcta agtcagcatt
      721 taattatcac atatctacta cttgtaatga ttttagactg ccgagtagtc tttcggcaga
      781 caaccctcac actcctctca tctaattaca aagagagggt atacctacaa agtcaattat
      841 caatgtaggt ataccccata tataagctgt atttaaaatt taattattta taagtgttta
      901 acattacttc ttcttgttta tatattttta ctccacgcct acttcattcc atgcttcata
      961 cacctgttca gctgtttgct cgtcatataa atctttagcc gcttggtata atgcatcttt
     1021 acaatctttg aagtttgaat tacttgttaa gtattccgtt aatgctcggt agtaaatttg
     1081 ttctgattta gatttcccta ttgcttgaat cacgttataa gctgctttat ttggaattcc
     1141 agaattcgta tgtacgccac cattatcttt ttcagtgaat acatagtctt tcatatgagc
     1201 tggttgacca aattgttctg ggtttgacat gctgcgtaaa gcgtctccct cttttccagg
     1261 tgtgtagaca tcttcaccca ttaagaaatc ctcgtcatct acaaagtatc caaaaacatc
     1321 tgaaaagctt tcatttagag cgcctgactg gtccttatat tctaagttcg ctgtctcttg
     1381 tgtcacaccg tgtgttaatt cgtgtgctac tacgtcattt gcacccgata aacttgtgaa
     1441 tgtgcgacca tcaccatcac catagatcat tttgtcaccg atccatgcgg cattatttct
     1501 gttatcttga ccaccgtagt tattaacatg cgttaatgaa acaattggac taccttggtt
     1561 gtcatatgat tcacgaccaa atgtgtcttt gtaataatca tatgtttgtt tagcgtaata
     1621 atttgcatct acgccagcac gttgctcatc ttttacgaag ttttcatctt cattagtaat
     1681 caatgttgct tgacctgttt gatcattaaa gctaaatgct gataatttac cttgatgcgt
     1741 taaatcttct aggctaaatc caccgtcaat actattgata ttgatatctt ttgtatcgcc
     1801 aagtacacct tttcctttac cagtttctgc agcttcttta actaagttca ttttttctaa
     1861 aatttcgcca gtttgagcat caattttaac tttccaatgt gaaatttctg gtgtcactgt
     1921 aattaactca acattataaa cgtatttatt actgtcacca tcgatttcaa ctttgttttc
     1981 tttaatgact ttatctttaa gatttttcgc tttattctta tcaatcttaa ctgctttaaa
     2041 tgctttgtcg gctgcgtcat ctttacttaa tgtcacttta ttcgttggct ttactttctt
     2101 cgcatcagta tccccattga ttaaaacgac ttttcctgat ttgtctgcgt gtactttcac
     2161 ttctttgtca ggtgcatgaa caccatcaac actcggttgc aatgtataat gcgtaaatcc
     2221 ttttttatca gtttttacat cagtaacagc gtaatcttga taaatatttt ttacattttc
     2281 ggatttaggc aattctttta atgctttgac cgcatcactc ttttgagtca cctcaaattt
     2341 aatatcagaa ttagctggtt tattttttga atcaatcgct aatgctgctg gtgataaagt
     2401 gctcaacaag gttaatgctg ccatacttgt aaatgcatat cttgaaaatt tcctcacatt
     2461 tcattcctcc tgaaatctta aaaacagctt ataaataaaa tattaattta atttttcaga
     2521 aaagtaaatg attatttcta tttatttcta tactttttgt tcgttgattt tattta
//
LOCUS       DANJFZ010000201         2497 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.207, whole genome shotgun sequence.
ACCESSION   DANJFZ010000201 DANJFZ010000000
VERSION     DANJFZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.207"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(6..620)
                     /locus_tag="PWN45_002756"
     CDS             complement(6..620)
                     /locus_tag="PWN45_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0624450.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            complement(637..1707)
                     /locus_tag="PWN45_002757"
     CDS             complement(637..1707)
                     /locus_tag="PWN45_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL0624451.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            complement(1719..2228)
                     /locus_tag="PWN45_002758"
     CDS             complement(1719..2228)
                     /locus_tag="PWN45_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624452.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
BASE COUNT          815 a          448 c          321 g          913 t
ORIGIN      
        1 actatttaat ttattaattt atttctaagt aataacgttg ataaaataat gcaaaataat
       61 ccaccaataa tgccggcaat aatatctgtt ggataatgta cacctagata tacacgtgat
      121 atggaaatca ataaaatcat agctgcacat aaccctataa gaataccttt tgaattacct
      181 tgatttaatc gatttaatag atagatacca cttccaaaat atgcagttga tcccatagca
      241 tgaccgctag gaaaactaaa tcctgttata tcaattaaac gcagcaatgt aggtctttct
      301 ctatcgaata tattttttaa tgctggattc aaaattccag ataatgccat tgttaatgca
      361 aaaaataatg cttcaatttt gtggcgcttt aacatgagat atgccacaag aagtaatgaa
      421 atacataaca ttgcccagac ttcacctact ttagtagccc caagcatgat agatgtcgta
      481 ataaagctct ctgatgaata tacaaactca taaacttcat tatcaatcca tttcccaagt
      541 cttgattcgt ggaaaaacgc gataattcca aaaaccaatg taaaaacgat gagcaaagag
      601 atacgtttcc attgactcat aaagcattac cccccattaa ttcaatgcgt ctttaatcat
      661 cttatcaaat aaagcttctt tcgtataact ttgttcatac gatttcatat tattgataat
      721 tcgagttctt tcctgttcca tttcatttag ttcttgtaat aaaatttgtg ctgttaattg
      781 ttcttcatca atcgctttag catatccttt atcagcaaaa tgatttgcat tgtcaatttg
      841 gtcgcctcgg gattgatcta aacctaatgg tactaataac attggtatac gtaatgttaa
      901 gaactcataa atcgcatttg atccagctct acttattact gtatccgtaa ttgctaataa
      961 atctgttaaa tcctctttaa caaattcata ttgtatatat cctgattttt taacttgagc
     1021 atcttttaat cctttaccag ttaaatgtat cacttgatat tgttgtaata atgcatctaa
     1081 gttttcgcga ataatgctat ttaatttttt acttcctaag cttccaccca taacgagtaa
     1141 aactttttta ttttcattaa agcctgttaa ttgataacca ttatgtgcat taccattttt
     1201 taaatcttct cgaattgttg ctccaataaa atcagctttc tctttaggta agtagtttag
     1261 cgtttcttca aatgttgtat atattttctt ggcaaattta agtgctatct tattcgctaa
     1321 tcctggtgtt aagtcagatt catgaataag agttggtata tttaatgatt tggctgcaat
     1381 aacaacaggc acagatacaa atccaccttt tgaaaataat agatcaggtt tttctttttt
     1441 caaaacttta cgagcatcaa gaataccttt caatacttta aatacgtctt tggcattttc
     1501 taaagaaata tatcttctta atttaccact cgaaatagga taatacttaa tttctggtag
     1561 ttgtgattca atcatttctc tttcaatacc atttttagaa ccaatataaa gcgcttcata
     1621 accttgtgat aatgcagttg gaattaaact taaatttact gatacgtgtc caactgttcc
     1681 ccctccggta aatgcgattt tcgtcattaa tattacctct attcttttaa atttttataa
     1741 tatgcataaa atggttcacc tttatcatat ggcggatatt ccattaattg ttcaccgacc
     1801 ttatgaaatc caaatttggc aaataaacct tgtgcaggtt tgtttaacgc aaaggtgtcc
     1861 gttaaaataa cttctgcacc acgtgcttta actacatcaa taacataatt gaataattct
     1921 gtagcagctc ctttatattc tttcgaacca gttaatcgat gaataacaaa ggcgccttct
     1981 ctatttactg gccagtcaat gtcatcatac cattctgctt ggtcttggtc gacaacaata
     2041 aagccataaa ttttgtcatt ttcctctaat acgtacaaat aatctttagc aatatcttct
     2101 tcaaaatgtt ctaaaagtgg gtactgatcg tcccattgct cgttgtcgtg ttctttcatt
     2161 aattcttttg cttcttctac tagatttaag atttgatcta aatctgacat tttacctaga
     2221 cggatcatgt acgtcactcc ttatatcatt atgttaaaca tgtttttcag tacatgttaa
     2281 tcttacacac tattaacatg ctttaaacct ttattatgaa tgaaaaatag taattatcac
     2341 actatgggat aaaaataacc aataaaagta tcatatacca attaattgat tgttaaaact
     2401 agtgaataat actatttaaa aagtatatgc tcattgcata ttaaaccatg aattgttatc
     2461 agcgatgata gaaattgcgt tatttacatc atttgta
//
LOCUS       DANJFZ010000202         2494 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.208, whole genome shotgun sequence.
ACCESSION   DANJFZ010000202 DANJFZ010000000
VERSION     DANJFZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.208"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            9..143
                     /locus_tag="PWN45_002759"
     CDS             9..143
                     /locus_tag="PWN45_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDL0624453.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(740..1525)
                     /locus_tag="PWN45_002760"
     CDS             complement(740..1525)
                     /locus_tag="PWN45_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDL0624454.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(1886..2482)
                     /locus_tag="PWN45_002761"
     CDS             complement(1886..2482)
                     /locus_tag="PWN45_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0624455.1"
                     /translation="MTFDNAMEERRSIYNLKDSISISDDELESMIAHAVKHVPSSFNS
                     QSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSYGTILFYEDQD
                     VVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYNPLVDEMTSNE
                     FNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT          843 a          407 c          363 g          881 t
ORIGIN      
        1 aatagttgat gagttgttta attttaagaa tttttatctt aattaaggaa ggagtgattt
       61 caatggcaca agatatcatt tcaacaatcg gtgacttagt aaaatggatt atcgacacag
      121 tgaacaaatt cactaaaaaa taagatgaat aattaattac tttcattgta aatttgttat
      181 cttcgtatag tactaaaagt atgagttatt aagccatccc aacttaataa ccatgtaaaa
      241 ttagcaagtg agtaacattt gctagtagag ttagtttcct tggactcagt gctatgtatt
      301 tttcttaatt atcattacag ataattattt ctagcatgta agctatcgta aacaacatcg
      361 atttatcatt atttgataaa taaaattttt ttcataatta ataacatccc caaaaataga
      421 ttgaaaaaat aactgtaaaa cattccctta ataataagta tggtcgtgag cccctcccaa
      481 gctcgcggcc ttttttgtaa tgaagaaggg atgagttaat catcattatg agacccgccg
      541 ttaaaatata tgaataagtc taatgttgga aaaggtcaaa aaattaatca atttaattaa
      601 gaaaatcatt catttgcaaa gggcgaaatg ggttcttact gagttatcta ttataaaaaa
      661 ataaacatag acttatgaaa aatctctcat aaatctatgt ttagtcatga catgtgttaa
      721 atattatttc gggcgcttct tatttataca aatctaattt aatactttta aatacaggta
      781 tattttcgcg ttgctgttct acttcattta agtttaaatc tacagtcaaa atatctgcgg
      841 attcatttaa ttctccaact aaatctccat ttgggtttat aactatcgaa tgaccagcat
      901 attctgtgtt accatcgaat ccagtgctat tagttccaat gacaaacata ttattttcaa
      961 ttgcacgtgc ctttagtaat gaatgccaat gttgaagacg tgacataggc cattgcgcca
     1021 cataaaatgc aattttagca ccactacgag caggatatct taataattct ggaaaacgta
     1081 aatcataaca gataagttgg gtcacataag taccgtcaga caattgaaag ggttcagcta
     1141 cgtattcgcc agcggttaaa aattcatgct ctcttaacat aggaactaaa tgaactttgt
     1201 cgtattcatt aatcagctgg ccacttttat tcacactaaa agctgtatta aatatttgat
     1261 tgtttctaat gttagaaact gacccagcta cgatatcgac tttatatttt tcagctaaat
     1321 gtttaataaa tgaaaaactt tgtcctagat tattatctgc tttttcattt aaatgctcta
     1381 aatcatagcc attattccac atttcaggta aaacgactac atctacttca gcattcatat
     1441 ttttttcgaa ccattgcgtt atttgagttt catttttaga actatctcca aaaacaatcg
     1501 gtaattgata aatttggact ttcataacat cacatccttg atagatctta tatataactt
     1561 actaaaagtt atgttgaaac gcaaaaaacg agcacaagac ataaaatcaa agtcctaggc
     1621 tctacaaagt tatattgaca gtagttgatg gggccccaac atagagaaat tggaacacca
     1681 atttctacag acaatgcaag ttggggtggg ctctaacata aagaaatact ttttctttag
     1741 aaattagtat ttcttataca tgagttttac tcatgtattc ctattcttaa gtgcacatta
     1801 gcagcggcta atgtgtaaga actactacat aatgaataac taatgattct ttatcatttc
     1861 tgtcccattc ctaacaatat attgattatt tttttattac gaaacgatct tccactggat
     1921 taaatgtttt ttcgccagca gcttcacgaa tatcaccaaa tggcatttga gcaataagtt
     1981 tccaactttt aggaatatta aattcatttg aagtcatctc atcaacaagt ggattatagt
     2041 gttgtaatga agcacctatg cctttagtag ctaatgcagt ccaaattgca aattgatgca
     2101 tggcatttgt ttgagttgac catattgcaa aattatcata gtagtttggc atttgttctt
     2161 gtaaaccact tacaacatct tgatcttcat aaaacaaaat tgtaccgtat gaatgtttga
     2221 agttatcaat tttttgttca gttggctcga aatcacgatt ctctcccatg acttctttta
     2281 aaattgcttt tgtgttatcc caaaatttat tattgttgtc atttaacaag agaacaattc
     2341 tagttgattg agaattaaat gatgaaggaa catgtttaac tgcatgtgca atcattgatt
     2401 ctaattcgtc atcgctaatt gatatcgaat ctttcaaatt atatattgaa cgtctttctt
     2461 ccattgcatt gtcaaaagtc attgcttttt tatc
//
LOCUS       DANJFZ010000203         2262 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.210, whole genome shotgun sequence.
ACCESSION   DANJFZ010000203 DANJFZ010000000
VERSION     DANJFZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.210"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..495
                     /locus_tag="PWN45_002762"
     CDS             <1..495
                     /locus_tag="PWN45_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0624456.1"
                     /translation="KKWIVYDKNIMEMPSRYYNIMENTGQVIYIQGQGMNKEFSAMIT
                     DILPNFQFIGNGKGFATYKGKDSLRLVDNISNSFKKKINLNSEEIVYYIYAILHHKYY
                     VNKYSSDLSKGFPRIPILKDVYGFVEIGRELVELHLNYEKQLNWDGVEIIYNNMNPNY
                     KVEK"
     gene            516..785
                     /locus_tag="PWN45_002763"
     CDS             516..785
                     /locus_tag="PWN45_002763"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0624457.1"
                     /translation="MDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQYNVSIDKK
                     SGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            846..1589
                     /locus_tag="PWN45_002764"
     CDS             846..1589
                     /locus_tag="PWN45_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624458.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            1590..>2262
                     /locus_tag="PWN45_002765"
     CDS             1590..>2262
                     /locus_tag="PWN45_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0624459.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDL"
BASE COUNT          905 a          253 c          406 g          698 t
ORIGIN      
        1 aaaaaatgga tagtgtacga caaaaatatt atggaaatgc caagtagata ttataatata
       61 atggagaata ctggacaggt gatttatata caaggacagg gaatgaataa agaattctcg
      121 gctatgatca cggacatatt acctaatttc caatttattg gtaatggcaa aggctttgct
      181 acatacaaag gtaaagatag tttgaggctt gtagataata tttctaattc ttttaaaaag
      241 aaaataaatc ttaattcaga agagatagta tattatatat atgcaatttt gcatcataaa
      301 tattatgtga acaaatattc aagtgatttg agtaaaggtt ttccgcgaat accgatatta
      361 aaggatgtat atggatttgt tgaaattggt agggagttag tggaattaca tttaaattat
      421 gaaaaacaac tcaattggga tggtgtagaa ataatttata ataatatgaa tccgaattat
      481 aaagttgaaa aatgaagcac cctaaaaagg gcgtgttgga tacagtaata tacaataccg
      541 atattacagt tactaacatt ccaaataaag tatataatta tattgtgaat ggcaagcctg
      601 ctattgaatg gattatagat cagtacaacg taagcatcga caagaaatct ggaatattag
      661 atgatcctaa tgaatttagt gaagatccga attacatttt aaatctgctt ttatcagtta
      721 ttactgtaag tatgaaaact cttggattaa tagataaatt accagatttt gaagtattag
      781 attaaggaga ggatatattg acaaatacta tcaaaaaaat aatttatttt gatgaactat
      841 cagcaatgga tttattacag atagagaaga aaggtaacct tactaaaaca gttcagttga
      901 tgcatgatga aaaaggtagt gcaagtggtg aatctaaagt gaacatggaa tttggtaagc
      961 aaagcgctgt aaaacaaatt tttgattctt tacttgggat gtcagcgagt gcttcggcta
     1021 gtgcaggtat aaatggaaat ataactggag agcgagtagc caaaacaatt ttagagaata
     1081 cattaatata tgattttttg gatacagtag agtttagaaa gaaaaaaact attattgata
     1141 tttcagaggg gtttaaacta aatattcaac aagatacgat gacatatttc gcgactatta
     1201 cccccatagc aagtatgttt gaaggatcta aacatctaga cgaggaggag ctatcatttg
     1261 atgtttcaaa aatgaatgaa gcgattagaa gtattaaagg ctattttgaa ttaataggaa
     1321 caagagggaa tgaaaacaga gtttttagat ttaatataga atcttttaaa aataactata
     1381 gaatacaaga tttaaaattt atgaatttaa cactctactc agtacttgtt ggtgaaataa
     1441 gcaaagaaaa tctaaattac gataaagagt ttgatctaaa tcaaggtgcc aaacctagtg
     1501 gaattggaaa gtttaaggga attagatcag aagcaactaa agaagataaa tatcaagtgt
     1561 atgatgttat tcttgcagga gtgaaataaa tggaaattca tgtttatacg ggaccgcttt
     1621 gtggatttaa tgaatatatt aattctgaag ttaaagaata tcaccgcttc atggaattaa
     1681 tcagagaata caatatcata gtgcgagcaa gtgatacatt tggaactcat gatgttgatg
     1741 gtatttttga tactgagata gaaaatcttg tgatattttc aaacgacttt gcttctgtaa
     1801 caactcatgt tattactaat tttataaata tcgttacttt aggtagaaat attaagaaaa
     1861 tctatattca gaatcctcca aaaagagtat ttcaatcact aaatagtttt cacgattctg
     1921 aaataataga aataaactat gaatatacaa cattaaaaaa agatgatgta ataaattttt
     1981 ataaagagca gatggctggt tctaaaataa taggtcaaac agacgctaaa aaacaaatga
     2041 gcatagattt atataaacat acttccttag aaaataataa accgaccgta atgctttact
     2101 atggtccatc aggtgttgga aaaacagaat tagccaaaga aatagcaaaa aaatataaag
     2161 gtaatatcac tagaatacaa ttttctatga tgcagaatga tgaatctttt aattatatct
     2221 ttggagatga acatgccaaa gtatcattgt caaaagattt ag
//
LOCUS       DANJFZ010000204         2230 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.211, whole genome shotgun sequence.
ACCESSION   DANJFZ010000204 DANJFZ010000000
VERSION     DANJFZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.211"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..322)
                     /locus_tag="PWN45_002766"
     CDS             complement(<1..322)
                     /locus_tag="PWN45_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624460.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWA"
     gene            449..1138
                     /locus_tag="PWN45_002767"
     CDS             449..1138
                     /locus_tag="PWN45_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDL0624461.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            complement(1237..1725)
                     /locus_tag="PWN45_002768"
     CDS             complement(1237..1725)
                     /locus_tag="PWN45_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDL0624462.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(1722..>2230)
                     /locus_tag="PWN45_002769"
     CDS             complement(1722..>2230)
                     /locus_tag="PWN45_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0624463.1"
                     /translation="TMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRL
                     QTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDF
                     PGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKI
                     TKEDVIST"
BASE COUNT          822 a          380 c          281 g          747 t
ORIGIN      
        1 ttgcccattt ttcaagcgca atcttcagtt gcgtagctat tcccagtttg cgaaattgtg
       61 gttcaacata tagcagttca atgttaacca tacttttttc attactaaaa tgtccccaaa
      121 taaacgctat taattgacct tcattttcat atatataaat tttatcattt gtatgttcta
      181 atcttgaaca tatcatctcg tacctaagag caatactcag tttagtacat ttatagttgg
      241 attctacttg cgacaataat gtctcatgta tattagctat ttgcttgata taattatgtt
      301 catctttatt aagtgtcctc attttcctct ccaatattaa tcgttatatt tatgcgttta
      361 atatgatatg attgacctta ttaatactat aaaatcattt ccattttaat ttggaattaa
      421 acatttttaa aggagcgctt aagtaacaat gaaaagtact gcgcaattaa caaaagaaaa
      481 taatgttaaa tcattaagat taagcaatac tgatagagaa atcttcgaga attatatgac
      541 ttatatgcgc tctgactttc gcgtcaaccc tcatgataca gagttaatta ttaaccgtat
      601 actcaaacaa ttattaagcg cagaacaaca tggtttatta gccttagatt ttttcaatca
      661 cgacccaaaa gcacatgcaa taaaagaact aaaagcgatg cctaatgaaa catttaaaaa
      721 tatttttaaa tatatctatc aacacatcgt tctattaatt gggattgtta gttttctcaa
      781 aggattttta ggatttttca tggaaaaaaa tggaagtaat ttatattttg tttcattccc
      841 gttttcagta gtcgttggat tctttattgt ctttttattt atatggttta gttttaaaac
      901 aatacaactg cagtgtttta acaattcaaa ttggatatgg atattcacct atttagcaat
      961 cattctacta attgtaggat ttttctatgt attctttatt ccacaatcat tacttgcctt
     1021 tggaccatat attcaggtaa gcaattgggt atttattata ttttcattta tagttatgcc
     1081 tattggatta cgcattgaaa gaaatatttc gaaaaaacat tctcatacat ttttataaac
     1141 aaaaagctta ttacatcata tgcgaatttg gtactgttaa tgataaaatc actaaattac
     1201 agttaccgaa aaacatattt tgcaataagc tttttattaa ttaatcaata aagcatctat
     1261 aaagaaccat acaatcatta taaacataat gattgcttga gctatactac ttgttaaaaa
     1321 tgctacgatt gaacctatac ttactttaac tgctctttca aatgaatagc cttgtattaa
     1381 ttcaactatg aataccaaaa taaaaggtat aataataatt ccaaatggcg gtaaaacaaa
     1441 acatccaata accacaccaa ttaaagctgc atactctcca aacttagaac caccgaagcg
     1501 attcacaaaa tatttattag ctaaaaagtc ggcacataaa attaatattg ttagcaatgc
     1561 catagataca taaaacaccc atgataaatg ctgattatga aagccaaatt gatagattaa
     1621 aaagccaacc cataatacta aaacagaagg aataatcggc ttaatcaacc caacaaatgc
     1681 taacatgaag gcagcgatga taagtagcca taaaataatt gtcatgttga tatcacatcc
     1741 tcttttgtaa ttttttgatt tttagtaaag aatataagta tcatacctaa catgattgat
     1801 gctgctgata caaaaaatac attttccaaa cctacccagt gactcatagc accacctaat
     1861 aggttaccgc ctaactgtcc aataaccatt gcattagaaa atagtgttga agcgtatcct
     1921 ggaaaatctg gtaagatatc ttggaaataa ctaataccaa ttcctaaaag aaccgctaag
     1981 aaaatagcta aaaacacctg tcctgctaac atcatataga agtttttaaa taccccaatg
     2041 ctgaagtaga ataaaccacc aaaaatcgct ccataaatca acaatgttcg agtctgtaat
     2101 ctagatgata aaacgccaag aatgatcata aatggcactt ctaaaccagc acataaacta
     2161 gctaaataac cgacatgttg ttcattttct tttaaataat cagtaacaaa taaaggcata
     2221 ttcatcgtat
//
LOCUS       DANJFZ010000205         2209 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.212, whole genome shotgun sequence.
ACCESSION   DANJFZ010000205 DANJFZ010000000
VERSION     DANJFZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.212"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            90..266
                     /locus_tag="PWN45_002770"
                     /pseudo
     CDS             90..266
                     /locus_tag="PWN45_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(244..345)
                     /locus_tag="PWN45_002771"
     CDS             complement(244..345)
                     /locus_tag="PWN45_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624464.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(468..563)
                     /gene="pepA1"
                     /locus_tag="PWN45_002772"
     CDS             complement(468..563)
                     /gene="pepA1"
                     /locus_tag="PWN45_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDL0624465.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            766..909
                     /locus_tag="PWN45_002773"
     CDS             766..909
                     /locus_tag="PWN45_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0624466.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1513..1896
                     /locus_tag="PWN45_002774"
     CDS             1513..1896
                     /locus_tag="PWN45_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624467.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            1907..2083
                     /locus_tag="PWN45_002775"
     CDS             1907..2083
                     /locus_tag="PWN45_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624468.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
BASE COUNT          836 a          297 c          381 g          695 t
ORIGIN      
        1 aagtgccgag gttttcttac ttatttagtt aattcaaagt ttatgccaga ttcataagaa
       61 tttgtgacac ttttaatagt gtaaccattg attattacaa tttatcaaat ggtcctttag
      121 aaggtataaa taacaaaata aaattaatta aacgtacatc ttttggttat ggaagttaca
      181 atcatttgcg caatcgaata ttattatgtt caaaacttta cgctccaaaa agtaaaaagg
      241 aagttaagca atgtttagtt gcttaacttc ggatattgaa cgcatcagtc caatttgaca
      301 tagagccttt tttagttctt gatgtttctc tttaaaacct tgcatatttt acaaaaagaa
      361 aaattagcag tataattaag acaacgaaaa taagtattta cttatacacc aatcccctca
      421 ctatttgcgg tagtgagggg atttttattg gtgcggctat atgtcaccta ttttgtattg
      481 cgtctactta gccaataaga aaaaaacgca atggcacagc cactgatgac tggtgctatg
      541 atgtgaacga aaataagcat caccttatac acctcctctc tgcgtctaaa ttgacgcctg
      601 agagataggc gactctacta ttatatcatc ggcaaatata caagcacagt cacttgcttc
      661 tgataagtta tatgattcta gctgatagat tgaatcgtct acacttaatt ggacaaattc
      721 tatgagaata gatattgtta atttaagaaa gtaggcgatt ttattatgac aagagaaaga
      781 agatcattta gttcagagtt taagttacaa atggttagat tatataaaaa tggtaagcct
      841 aggaatgaaa ttatacgcga gtatgatttc acaccttcga cgtttgtaaa tggcggttat
      901 aaaatgtagg aaaatggata aagcaacatc aaaacacggg tacattcaat caccaagata
      961 acttatcgga tgaagaaaaa gagctgatta aattacgcaa agaagttcaa catttaaaaa
     1021 tggagaacga tattttaaag caagtagcgc tgattatggg gcaaaaatag aagtcattca
     1081 aaagaatgca catcaatatt cagtatcagc aatgtgtaaa gtcctgataa tactaagaag
     1141 tacctattat gattctataa aaagaaaaga taataaaatc actaaagatg attcaaacat
     1201 agaacatgcc gtcataaata tttttaattc taatagaaaa gtctttggta caagacgaat
     1261 taaaaatcat ttaaatgaca agggtctcac tgtatctgga caaaagatag gtcgatcatg
     1321 aaaaaatcta gtttctgttt atacgaaatc taaatacaaa aatcatctaa aagaaactaa
     1381 tgaaaaacga attaaaaatc tttattatta gctgctggtg tattatttgt tagtccaatt
     1441 tcattatctt ataattcaga tgtagctcat gctgaagata agttagacca ttctcaagca
     1501 aaggtaatat atttgagtaa ccaaaattta tttgatgaac ttgagaaaaa aggttataaa
     1561 ctggaagata tatttacaaa agaagaaata aaaaaatata aagctgaaga ccaattgaga
     1621 gcgggtaaaa ctcaatatgt agaaacaggt aaagatactg caacattata tctttcttct
     1681 gcatatacaa aaacaatagc tgctttaggt gcgggggcta ttagcgttat ttctgcacta
     1741 acaggaggtt tagttggcgc aggtgttggt ggtttccttg gatctatagc tgcatcgaat
     1801 attgatacta gtaagggaat atatataaaa ttaaaaacta aaaagaatgc agccggggaa
     1861 tacgttctga caggagaaaa atggggatat cagtaagggt gattttatgg atagattaaa
     1921 atattcactt aaagttggaa ttttagcatt attattattt tgtactttaa attatttagt
     1981 tccaatgcaa agcaatgctt tttcaataat tatatattcg gcaatttttg ctgtgttact
     2041 tatgctttta gtttatatat ttttaggaat tttaaagaaa tgacatgaaa caaattagca
     2101 ttggctatga agaaatctat ggggatagaa tttttcatag ccatttttta aaagagcatg
     2161 aagtaaaaaa ttggaaaacc gtcaatcaaa taattgaaaa agaacactt
//
LOCUS       DANJFZ010000206         2208 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.213, whole genome shotgun sequence.
ACCESSION   DANJFZ010000206 DANJFZ010000000
VERSION     DANJFZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.213"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..297
                     /locus_tag="PWN45_002776"
     CDS             <1..297
                     /locus_tag="PWN45_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0624469.1"
                     /translation="VFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCG
                     GLSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            385..1002
                     /locus_tag="PWN45_002777"
     CDS             385..1002
                     /locus_tag="PWN45_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0624470.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            1077..1706
                     /locus_tag="PWN45_002778"
     CDS             1077..1706
                     /locus_tag="PWN45_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDL0624471.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            1763..1879
                     /locus_tag="PWN45_002779"
     CDS             1763..1879
                     /locus_tag="PWN45_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624472.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            1902..>2208
                     /locus_tag="PWN45_002780"
     CDS             1902..>2208
                     /locus_tag="PWN45_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0624473.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     "
BASE COUNT          818 a          255 c          336 g          799 t
ORIGIN      
        1 gtgttttata tatatttaaa tcagaaagta aagttatcta tctataatga tcggagtagc
       61 aatggaaaaa ttatgatttt tccatcattc aagaatcttt gctttgtact attttcttat
      121 tttttctgtg gtggattatc aatcatgttc ttagatgttt tgattagttt atctatccaa
      181 aatattatag tatttattgc ttgggttatt atgacaatgc tatttttctt tataaatatg
      241 tcttcaataa tagacaaaaa gattcatgtt atatatttaa ggtcatataa atattaattt
      301 tgatacaaaa gggcacaagt gttttgagaa acagaaacga taatatagct gtgtgaattt
      361 gttattaata ggaagtgata attattgctt tgtgaaaccg aaattatcaa taaaaatccg
      421 aaatacagag ttattaagta tgatgatgag tatttaatgg tcgatgtaat aagaacttgg
      481 cttgtatact tttttccatt tattaattgg ttcattccaa aaaggtgcgc caaaattagt
      541 agagaagagt atgaaaaact gaacactgtt aagccagtta aaaataaaaa tttttggcct
      601 gttgtaggag gaacaatcct actaggtgct acttccagaa agtacataca cttacttaat
      661 attcaattag aaaaaagatc ggttatattt atatgcttcg ttgtatttct atgtatttta
      721 atcttttttg tgcttctgaa tcgaaaatta aaattgaaag ttttcgataa taaaaaagaa
      781 gaacaaaaaa taattttagt accaacatta aaaaatgctg ttcttatatt atatggctat
      841 ttgttgattg gaggcatgtc aatattagct ttaagcatgt tgctgacatt agaaaaccag
      901 aatttaataa cttttatagc ttggggtatg gggttaatgt tgtttttctt gatgaatata
      961 acactcattg tcaataaaac agtcaaagtt ataaaaaggt gaataaataa ctaattatat
     1021 tgtattggcc gttcttcaaa taaaaaataa tttgaataag gtggaagtga taaacagtgc
     1081 tttgcgaatc tagagtcatt aatcaaaacc ctaaatatag aattattaaa tacaataatg
     1141 aatatttcat ggttgattta gtaagtactt ggattgctta ttttttgccc atgattaatt
     1201 ggtttattcc caaaaagtac gcgaaaatta gtagagaaga atttgaaagt ttaaatattg
     1261 tcaaacccgc taaaaataat actttctggc ctgttgcagg atttgcagtg ttattaacaa
     1321 ccttaacaag aaaatatatc tatttgctta acatccattt agaaaaagaa atagttatat
     1381 taacatgctg tatgatactt ctaggtgttt tcgcattgtt tatatatata aatacaaaat
     1441 tgaagttaca tatttttgat aaaaataaaa gtaataacga aaagatcata ttaataccta
     1501 catttaaaaa tatttgttta tccttatttg cttatatatt atttggtgga ttgtcaacaa
     1561 tggctctgag tatgttagta acttcatccc ctcaaaatat aatagaattt cttgctttaa
     1621 ttggcatgac tgcatgcttc tttctactga atatgtcatc ggttctagat aaaaaaattc
     1681 atgttatttt aaaaacaaat aagtagtaaa attgattaac ttaggtagta tcggatactt
     1741 aaatgttggt tcataaaaag caatgatttt aaatcgagga gctatcttag aacagggaaa
     1801 taaaacagcc aaagttataa aaagtgaatt aataactaat tatattatgt tagccacgct
     1861 tcaaataaaa aataattaga ataaggtggg attgataatc aatgctatgc gaatctaaaa
     1921 tcatcaataa aaaccccaaa tatagaatta ttaaatataa tgatgagtac ttaatgatcg
     1981 atataataag cacttggatt agtttatttt ttccttttat taattggttc atccccaaaa
     2041 gatacgtcaa aattagtaga gaagagtttg aaaatttaaa tattgtgaaa cctgctaaaa
     2101 agaatgtttt ttggccagtt gcaggtatct ctactttatt cgcagttaca ttaagaaagt
     2161 atacacattt acttgacact caacttgata gaaaattagt tattgcta
//
LOCUS       DANJFZ010000207         2184 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.214, whole genome shotgun sequence.
ACCESSION   DANJFZ010000207 DANJFZ010000000
VERSION     DANJFZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.214"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..168
                     /gene="sarT"
                     /locus_tag="PWN45_002781"
     CDS             <1..168
                     /gene="sarT"
                     /locus_tag="PWN45_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDL0624474.1"
                     /translation="IDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQHNYIK
                     NLLSEININET"
     gene            474..2184
                     /gene="sasG"
                     /locus_tag="PWN45_002782"
                     /pseudo
     CDS             474..2184
                     /gene="sasG"
                     /locus_tag="PWN45_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          889 a          282 c          426 g          587 t
ORIGIN      
        1 atagaccaat ctagaattgt taaaagcgta aaagatttat caaaaaaggg ttacttgaat
       61 aaatgtagag acccacatga tagtagaaat gtaatcattg ttgtttctgt aaaacaacac
      121 aattatatta aaaatctttt gagtgaaata aatattaatg aaacataaag tatgtttcga
      181 gattttaata tcttggtgca gttaaatgta ttttttaaat gcgttaaggt attttttatt
      241 ttagaaattc aatgctattg agttgatggg tttttccaac agataagaat gaattattca
      301 ttgaaaacta aaaataataa ttttatttgt agattgtttt aagtgcgtca atatataaaa
      361 taattttata aagataagtg ttatattcca ttgtgcaact cctattgtat ttgtaaaatt
      421 acatgtaaat tcactgtaag caaagtggaa aatatggaaa aaggagtatg caaatgagag
      481 ataagaaagg accggtaaat aaaagagtag attttctatc aaataaattg aataaatatt
      541 caataagaaa atttacagtt ggaacagcat ctattttaat tggctcacta atgtatttgg
      601 gaactcaaca agaggcagaa gcagctgaaa acaatattga gaatccaact acattaaaag
      661 ataatgtcca atcaaaagaa gtgaagattg aagaagtaac aaacaaagac actgcaccac
      721 agggtgtaga agctaaatct gaagtaactt caaacaaaga cacaatcgaa catgaaccat
      781 cagtaaaagc tgaagatata tcaaaaaagg aggatacacc aaaagaagta gctgatgttg
      841 ctgaagttca gccgaaatcg tcagtcactc ataacgcaga gacacctaag gttagaaaag
      901 ctcgttctgt tgatgaaggc tcttttgata ttacaagaga ttctaaaaat gtagttgaat
      961 ctaccccaat tacaattcaa ggtaaagaac attttgaagg ttacggaagt gttgatatac
     1021 aaaaaaaacc aacagattta ggggtatcag aggtaaccag gtttaatgtt ggtaatgaaa
     1081 gtaatggttt gataggagct ttacaattaa aaaataaaat agattttagt aaggatttca
     1141 attttaaagt tagagtggca aataaccatc aatcaaatac cacaggtgct gatggttggg
     1201 ggttcttatt tagtaaagga aatgcagaag aatatttaac taatggtgga atccttgggg
     1261 ataaaggtct ggtaaattca ggcggattta aaattgatac tggatacatt tatacaagtt
     1321 ccatggacaa aactgaaaag caagctggac aaggttatag aggatacgga gcttttgtga
     1381 aaaatgacag ttctggtaat tcacaaatgg ttggagaaaa tattgataaa tcaaaaacta
     1441 attttttaaa ctatgcggac aattcaacta atacatcaga tggaaagttt catgggcaac
     1501 gtttaaatga tgtcatctta acttatgttg cttcaactgg taaaatgaga gcagaatatg
     1561 ctggtaaaac ttgggagact tcaataacag atttaggttt atctaaaaat caggcatata
     1621 atttcttaat tacatctagt caaagatggg gccttaatca agggataaat gcaaatggct
     1681 ggatgagaac tgacttgaaa ggttcagagt ttacttttac accagaagcg ccaaaaacaa
     1741 taacagaatt agaaaaaaag ttgaagagat tccattcaag aaagaacgta aatttaatcc
     1801 ggatttagca ccagggacag aaaaagtaac aagagaagga caaaaaggtg agaagacaat
     1861 aacgacacca acactaaaaa atccattaac tggagtaatt attagtaaag gtgaaccaaa
     1921 agaagagatt acaaaagatc cgattaatga attaacagaa tacggacctg aaacaatagc
     1981 gccaggtcat cgagacgaat ttgatccgaa gttaccaaca ggagagaaag aggaagttcc
     2041 aggtaaacca ggaattaaga atccagaaac aggagacgta gttagaccgc cggtcgatag
     2101 cgtaacaaaa tatggacctg taaaaggaga ctcgattgta gaaaaagaag agattccatt
     2161 cgagaaagaa cgtaaattta atcc
//
LOCUS       DANJFZ010000208         2146 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.215, whole genome shotgun sequence.
ACCESSION   DANJFZ010000208 DANJFZ010000000
VERSION     DANJFZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.215"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            162..365
                     /locus_tag="PWN45_002783"
     CDS             162..365
                     /locus_tag="PWN45_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDL0624475.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(362..598)
                     /locus_tag="PWN45_002784"
     CDS             complement(362..598)
                     /locus_tag="PWN45_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624476.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            823..1404
                     /locus_tag="PWN45_002785"
     CDS             823..1404
                     /locus_tag="PWN45_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0624477.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1477..2094)
                     /locus_tag="PWN45_002786"
     CDS             complement(1477..2094)
                     /locus_tag="PWN45_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL0624478.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
BASE COUNT          785 a          328 c          326 g          707 t
ORIGIN      
        1 atactaaaaa ctcttaaagt tgtataattc gttaatattt ttagaatcag atatcaaaaa
       61 aatgtgcaaa atcacttttg cacatttaca aatactagtt ttctgataag attaataata
      121 acactttaaa tagcgcttaa taaatgaagg gggcaagtct tatgacgttt tacaatttca
      181 tcatgggttt tcaaaatgat aacacaccat ttggtatatt ggccgaacac gttagtgaag
      241 ataaagcatt ccctcgatta gaagaaagac accaagtaat tagagcatat gtgatgtcta
      301 attacacaga tcatcaatta attgaaacta caaatagagc tattagctta tatatggcaa
      361 attaatttga gtagtaccaa ttatgatgta ttagtgcatc ccaaatatct tttgttttaa
      421 agtttatttc atcatttctt atcgaaaatg gtgtaataat gtctttatct aaccaagtgt
      481 tgataagttc atttggtaca ccatctaaca acatttcact tttactaatt ataaaacatt
      541 cccagtcaag tgaaacattt tgtggattca cataattaca ttgattatga ttatccataa
      601 acactcactc ctttaaaatt ctgtactctt cattgcgttt taccccgtca cattatcttt
      661 taaactaaaa tcatcattac ttatgaaaaa aatgtacatc aaaagcaaag gttttcgcta
      721 ccgaaaaagt ttaaataatg gtttaatata tttggtactc attttaataa aaagagaata
      781 cattttgagc tatcaatact ttttattgaa gaggtgttat tgttggctaa aacgttatat
      841 ataatgcgcc acggacaaac tttgtttaat tttaagggac taattcaggg atttggagat
      901 tcgccgctaa cagaacttgg aattgctcaa gctcaaaagg cacgtagtta ttatgaaact
      961 aaggggataa acttcgattt atatgcatca tcaacgcaag aacgcgcaag tgacacactt
     1021 gaaaatgttg cacctaacca atcgtatcaa cgttttaagg gactgaagga atggcatttt
     1081 ggaatatttg aaggtgagtc agtctatcta tttgataatc tatacaagcc tgaggactta
     1141 ttcggagatc gaattgttcc tttcaaagga gaggcaaggc aacaagttga agaacgcatt
     1201 gtgaaaactt tacatgacat tatgtctcaa acaaagaata atgcattagt cgtgagtcat
     1261 ggaacaataa tgggagtatt tttaagatat tgccttaaac tagatgaagc attaaagcat
     1321 aatatcggta attgtaatat cctgaaattt gaatatgaca atggaacatt taaatttgtt
     1381 gagttaattg atccaaattt ataataaata aagcgttata catagacaca agtatgtctt
     1441 ttgtataacg cttttaaaat acaactacat aacgctttag aataataatt gaataagttt
     1501 ttgtaatatc atcagagcaa caataataat tattatgctt gatattttat tgattattgt
     1561 taataatttt ccggttttat caattgatcc aaccattttt cctaaaattg cgagtaaaaa
     1621 gaaccataac catgaaacgc taatacaagc gattgtaaac gcaattttat tgctgccact
     1681 atataatgca gcactactac caattactcc aattgtatct aaaatagcat gtggattgag
     1741 taatgaaact gataaagcaa aacttacttg tttcattgga gacataattt gagtttctcc
     1801 atctgttgag ggtttatcat gccaaatggt ccaagccata tacatcaaga aaattaaacc
     1861 aactatataa ataattgctt gaagtacagg taaagacata ataatgatag atactcctac
     1921 cactgcaata ataataagta agctgtctga caacccggct gtaattattg caggcaatac
     1981 atatctatat tttggttgat tagctccttg gttaaaaata aatacatttt gtgcacctaa
     2041 aggtaaaatt agaccaatcg ctaagataaa tccatgaata attgcggtta ccattaagtt
     2101 ttgacacccc taaaacatag aatattctga aaaaacttat tgttat
//
LOCUS       DANJFZ010000209         2082 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.216, whole genome shotgun sequence.
ACCESSION   DANJFZ010000209 DANJFZ010000000
VERSION     DANJFZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.216"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(922..>2082)
                     /locus_tag="PWN45_002787"
     CDS             complement(922..>2082)
                     /locus_tag="PWN45_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0624479.1"
                     /translation="FPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQ
                     NIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLN
                     QHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIF
                     EEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWEN
                     KFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAK
                     WGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHA
                     NLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI"
BASE COUNT          623 a          334 c          250 g          875 t
ORIGIN      
        1 tagttcttca atcttagtat taagtctaat tagtgtatca agaaattttt cttgattcgt
       61 tgatcgtttc aagttttaag tctcctttta aaatgaatat ttcacttctt ataactaata
      121 tttaaacaaa atgatcacat aataatattt aatttacaat atgccactac agtgtcaaca
      181 ttttataaac actattcatt tgaacatggt ctaaaacgaa taggacattg taaatgatta
      241 aatagtctgt ctctagttga tgtatatggt catttgttat gcagataata attaatctaa
      301 ttaattttta cgttttagtt acttggttgt gattgaggct ttttgtatct agttgtaata
      361 ttctcctttt ttagaatcat aataggtact tcttgaaatt tttagaactt tgcacattgc
      421 tgatactgaa tattgatacg cattttgttg aaagacttca ttttttgtct caaaattagc
      481 actgcttgct ttaaatcatc attttacatt atcaatcgat gtttttcttt gcgtaatttt
      541 attaactctt tttcatcact tgttaaaatg tcttgatgat tgaatgactc agtattttgg
      601 ttttgtttta tctaatttga gaaagttaat gatgttagat catattctcg tataatttcg
      661 tttctaagct taccattttc ataaagttta attattttaa tttaaattat ttactaaaag
      721 ctcttcagtc tcttgtcata ataaaatcgc ctaccctctt agtttaacaa tgtctattct
      781 catagaattt gtccaactaa atgtagacga ttcactaaac ttctaaaaaa ataaacccca
      841 aattataata gtctcaatat taatatatta caatttattc ataggatttt tatcatttca
      901 attaataggt tgttatcagg gttaaataaa cattttttgt aatagtcctt ttttacgttc
      961 ttttaataac tcaattctat tcatttgatt attcatttta ttgtctatag attttaacaa
     1021 tgcacttact ttatcttgtt cagtgagaca aggtatttta agatttatat tgcataagtt
     1081 cgcatgagtt aatttagctg gtgcattacc tgtgacaaat gctcgtagtt ctttaaaatt
     1141 taaataataa ttcataaaaa acaaattatg atcattactt ttaactacat gcgcatgatt
     1201 atttacccag tattgcccat tagcaataaa gctactcgtc tcaaactgcc cccattttgc
     1261 accatcttct cctattagta atcgttcttc attattgaat aaataatctt ttacgtaatc
     1321 aataattcca gttgcaccat agtaagggta taaccccttt tctcttaatg aagaagtaat
     1381 tggttttctt ctattatttt caaagataaa gatgtctttt atgaatttgt tttcccactc
     1441 tggatattct tcaccattct catctttgaa tcgcaattct tgtgtgaaga ttttctgcat
     1501 atagcctttt ttctgttgtt gaagcaattc aagtttttgt tcttctaatt caatttgtcg
     1561 gtcgagtttg ctgaagaact tgcctatttt ttgctgttct tcaaatatag ttggggtgaa
     1621 gatttttaaa ttagcaattt ctttgaagtt tagaccttct cgactacctc cactttgtgc
     1681 aaggaaaatt ttccttttac cttttcttga taatagatac tgtccaaaaa aattataata
     1741 atactctttt ttcaatctaa taatacatac atgttgattt aaattagcat gcgtttcaac
     1801 tatcgaatta atggctgttc tacctattga tgctcctgta atatttaaaa gaacatcacc
     1861 atagtacgtt ctactatttt tcatctcatc atctatatct ttactaatat aaactaagtc
     1921 attaagattt aatttaccat ttctaatatt ttgactcctt aaaaatggta tgcctttgtt
     1981 tgtatagttt tcacttccac ctttgggagt ctttccacta cctattttgg tagtaagatt
     2041 ccctaacttc ttctcttccc attcgccttc aaatcctggg aa
//
LOCUS       DANJFZ010000210         2062 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.217, whole genome shotgun sequence.
ACCESSION   DANJFZ010000210 DANJFZ010000000
VERSION     DANJFZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.217"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            84..191
                     /locus_tag="PWN45_002788"
                     /pseudo
     CDS             84..191
                     /locus_tag="PWN45_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            303..1097
                     /locus_tag="PWN45_002789"
     CDS             303..1097
                     /locus_tag="PWN45_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDL0624480.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(1101..1223)
                     /locus_tag="PWN45_002790"
     CDS             complement(1101..1223)
                     /locus_tag="PWN45_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624481.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1658..1849
                     /locus_tag="PWN45_002791"
     CDS             1658..1849
                     /locus_tag="PWN45_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624482.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT          790 a          278 c          359 g          635 t
ORIGIN      
        1 ttgatgtttc ttgaactata tatcatctag tcgtcattta caattggtaa atatgacttc
       61 aaactgtatg aaggtgatgg cgattaaaag gctcatccgt aggttctaaa gaactagaat
      121 ttaactttat aagaaataaa gaagaaaatg tgtatttttc tgatagtatt aatttcaaac
      181 ctactgaata gggttaatag aagtttgaaa taaaaatttc ataaagtgtt caaaataatt
      241 tttcgcttta gttgtaaata ttaaaaactg agtcaatatt aaaaatataa ggtggagaaa
      301 tgatgattca ttcaaagaaa ttaacgctcg gtatatgctt ggtattactc attatattga
      361 ttgtaggtta tgtcattatg acaaaaacaa atggtcgaaa cgcccaaatt aaagacacat
      421 ttaatcaaac attaaaatta tatccaacca aaaatctcga tgatttttac gataaagaag
      481 gctttcgaga tcaagaattt aaaaagggtg ataaaggtac ttggatagtt aattctgaaa
      541 tggtaatcga gccaaaaggt aaggatatgg aaacgagagg aatggtgctc tatatcaatc
      601 gcaatactag aaccacaaaa gggtattatt ttataagtga aatgacagat gacagtaacg
      661 gcagaccaaa ggatgatgaa aaaaggtatc cggtaaaaat ggaacataat aaaatcatac
      721 caacgaagcc actaccgaat gacaagttaa aaaaagagat tgaaaacttt aagttctttg
      781 tacaatatgg caactttaaa gatattaatg attataaaga tggtgatatt tcatataatc
      841 ctaatgtacc aagttattcg gcaaaatatc aattgaataa tgatgattat aatgtccaac
      901 agttaagaaa aagatatgat attccaacca aacaagcgcc gaaactatta ttgaaaggcg
      961 atggagattt aaaaggttca tccgtaggtt ctagaagtct tgaatttacc tttgtcgaaa
     1021 ataaagaaga aaacatttat tttacagatt ctgtacaata tactccaagt gaggatacaa
     1081 ggtatgagtc aaactgaata tcaaataaaa cctggtaata taacaagtaa ctctgaagaa
     1141 acaagttcga tatctaaagt gagctgtgaa atataggtag ccatttcaaa aaatttaaag
     1201 gtgaatttga taatgtagct caaggagatt gggttaaaaa ggcgaagaat gaactggatg
     1261 atattagtaa gaaattaaaa aatattcaaa gaacggaagt ttaatagctt atatgattct
     1321 tggagctaag acagcatgcg ttcattcatg ccattattaa tataagcacc gcaacaaaaa
     1381 agcttctaat gtgatacagg aacctcatat tccgtatcat gttagaagct tttaatgtct
     1441 aaagaacatc tacattttat catattttct gacttattaa acttttatat aattaaatat
     1501 ttcttaattt tccaaaatag tgataaattt gtgaaataca tcacaaatcc ctttatttat
     1561 ttggaaattc atgtaatatt agacttgtaa gaagttaata aatagagaga gacgagagag
     1621 tttatataaa tactatataa acattggagt gatgattatg agaaaagaga ttgaagcgct
     1681 tattttctca gacgtaagta gctatgatat ttacgtgaac actggtgtaa atcaaggatt
     1741 aattggtgac atcaaagatg gttacctaac tattgattct atgccttaca ttgatgctga
     1801 gcgtttgtat cactttgcta tggaacgtaa atcgttagtc actaactagt tcttattgcc
     1861 aatgattact acccctagtc ggcggcaatt gaagtgtgat tgatgtaact tgccctcgtt
     1921 ggtgagcaat tgagggcaga cccctttaat taagtaaacc ctaactcccc acaaatctgg
     1981 aacgatacta aaagccacgt cctatattgg atgtggcttt agtcatactt atattatttt
     2041 taaaacgatt acctacaaga tt
//
LOCUS       DANJFZ010000211         2056 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.218, whole genome shotgun sequence.
ACCESSION   DANJFZ010000211 DANJFZ010000000
VERSION     DANJFZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.218"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            135..2056
                     /gene="eap"
                     /locus_tag="PWN45_002792"
                     /gene_synonym="map"
                     /pseudo
     CDS             135..2056
                     /gene="eap"
                     /locus_tag="PWN45_002792"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
BASE COUNT          911 a          254 c          299 g          592 t
ORIGIN      
        1 agaactttta acagtgttaa aaaattattg ttaaagataa tttattatta atattcagtt
       61 aattcaaaaa atagagaaag tctggctata attaagttgc aaatacgaat tacacaaaaa
      121 aggagagata atttatgaaa tttaagtcat tgattacaac aactttagca ttaggtgttt
      181 tagcatcaac aggtgcaaac tttaataata atgaagcgtc tgccgcagct aagccattag
      241 ataaatcatc aagttcgtta caccatggat attctaaagt ccatgttcca tatgcaatca
      301 ctgtgaacgg tacaagccaa aatattttat caagcttaac atttaataag aatcaaaata
      361 ttagttataa agatttagag gatagagtta aatcagtttt aaaatcagac agaggtatta
      421 gtgatataga tttaagacta tcgaagcaag cgaaatatac tgtttacttt aaaaatggaa
      481 caaagaaagt tatcgatttg aaagcaggta tttacacagc tgatttaatt aatacaagtg
      541 aaattaaagc tattaatatt aacgtagata ctaaaaagca agttgaagat aaaaagaaag
      601 ataaagcaaa ttaccaagtt ccatacacaa tcactgtgaa cggtacaagc caaaatattt
      661 tatcaaactt aacatttaat aagaatcaaa atattagtta caaagattta gaggataaag
      721 ttaaatcagt tttagaatca aatagaggta ttaccgatgt tgatttaaga ttatcgaagc
      781 aagcgaaata tacagttaat tttaaaaatg gaacgaagaa agttatcgat ttgaaatcag
      841 gtatttacac agcgaattta ataaattcaa gtgatattaa aagtatcaat attaacgtag
      901 atacaaaaaa acatatcgaa aataaagcta aaagaaacta tcaagttcca tattcaatta
      961 atttaaatgg tacatctaca aacattttat cgaatctttc attttcaaat aaaccttgga
     1021 caaattacaa aaatttaact agtcaaataa aatcagtact gaagcatgat agaggtatta
     1081 gtgaacaaga tttaaaatat gctaagaaag cttattatac tgtttatttt aaaaatggtg
     1141 gtaaaagaat cttacagttg aattcaaaaa attacacagc aaacttagtt catgcgaaag
     1201 atgttaagag aattgaaatt actgttaaaa caggaactaa agcgaaagca gacagatatg
     1261 taccatacac aattgcagta aatggcacat caacaccaat tttatcagat ttaaaattta
     1321 caggtgaccc acgtgtaggc tacaaagata tctctaaaaa agttaaatca gtattgaagc
     1381 atgatagagg tatcggggaa cgtgaattaa aatatgcaaa aaaagcaact tacacagtac
     1441 attttaaaaa tggaacgaaa aaagtgatta acataaattc aaatattagc caactgaatc
     1501 tgctttatgt tcaagatatt aaaaagatag atattgatgt taaaacagga actaaagcga
     1561 aagcggatag ctatgtacca tatacaattg cagtaaatgg cacatcaaca ccaattttat
     1621 caaaacttaa aatttcgaat aaacaattaa ttagttacaa atatttaaat gacaaagtga
     1681 aatctgtatt aaaaagtgaa agaggcatca gtgatcttga cttaaaattt gcgaaacaag
     1741 caaaatatac agtatatttc aaaaatggaa agaaacaagt agtgaattta aaatcagaca
     1801 tctttacacc taatttattt agtgccaaag atattaaaaa gattgatatt gatgtaaaac
     1861 aatacactaa atcaaaaaaa aataaataaa tctaataatg tgaaattccc agtaacaata
     1921 aataaatttg aaaacatagt ttcaaatgaa tttgtgttct ataatgcaag caaaattaca
     1981 attaatgatt taagtataaa acttaaatca gcaatggcaa atgatcaagg gataactaaa
     2041 catgacatag gacttg
//
LOCUS       DANJFZ010000212         2022 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.219, whole genome shotgun sequence.
ACCESSION   DANJFZ010000212 DANJFZ010000000
VERSION     DANJFZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.219"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..1061)
                     /locus_tag="PWN45_002793"
     CDS             complement(<1..1061)
                     /locus_tag="PWN45_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0624483.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKV
                     VRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKF
                     KINSQGLTSDTWNLKYKQL"
     gene            complement(1054..>2022)
                     /locus_tag="PWN45_002794"
     CDS             complement(1054..>2022)
                     /locus_tag="PWN45_002794"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL0624484.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          559 a          393 c          295 g          775 t
ORIGIN      
        1 tttaattgtt tatattttaa gttccatgta tctgatgtta atccttgtga attaatttta
       61 aatttatgaa tcattctatg tgttttaaac ttatatccaa taaataatga gctagtattt
      121 tgtgttggat aaagcacagt atatgcaggg ctaacaatcc cattataatt tgatttacca
      181 ctagcccctt gccacattct catagaatta tatgcaatat catttttcct aactacttta
      241 taattacttt tatctttact tgaattatct tttctatcca attcactaaa ttttataatg
      301 ccactattta tagttactga aagcatttgc cctttgtcag aacgttcgtt tctctctttt
      361 aaatattttt ctattttgct attttcccaa tctggataat cttcaccatt ctcatctttg
      421 aatcgcagtt cctgtgagaa aattttctgc atatagcctt ttttctgttg ttgaagtaat
      481 tcaagctttt gttcttctaa ttcaatttgt cggtcgagtt tgctgaagaa cttgcctatt
      541 ttttgctgtt cttctaaact tggatattta attagaatag taaaaaaatc attcacagaa
      601 acatttaata atccgtgatt tcttgcaccc tcaactgcaa ttccagaaac ttctctatac
      661 cagtgtgtcg aatcaaaata tgcttccatg aagtctttag acatttcact tttaatagaa
      721 aaacaaatat acaaagagga caatacacca ctatcatatc tagttaatct tttaatagcc
      781 cctaatgggt atccattaga ataacttttg ttatacgcga attctccatt ctttattagt
      841 gtataatttt ctagattttt cgacgaaact gatttactaa aatattctgt ttgatcaatt
      901 aaacctaact gtccggatat tgttaaaggc tttttcgatt ctaagttttt atttttccta
      961 attactctat ctgtaagatc ccctaactgc ttctcttccc attcgccttc aaaccctggg
     1021 aacctcaatt ctggcacatt tttcttttgt gtattactca tctttcaaca ccccaagttc
     1081 tttcaggtat gcattgattt cttgctcaat ttctgcgatt tctttatcga tatttttcaa
     1141 atcttgttgg acttgatcta aatcaatcgg tgcttcttct tcgaatgtat cgacatatct
     1201 cggtatattt aggttgtaat cgttatcggc aatctcttgt agtgtcgcgc tgtagctata
     1261 tttatcaatt gtttccttac gcttatatgt gtctataata cgttcgactt gggcatcgct
     1321 taaatgattt tgattttttc ctttttcaaa atcattggat gcatcgataa atagtacgtt
     1381 gtcgtcttgt tggcgacatt ttttaaatac taaaatacat gttggaatac ttgtcccata
     1441 gaaaatattc gctggcaaac caatcacagc ttctaagtag ttcttttctt caattaaata
     1501 acgacgaatg acaccttctg cagcacctcg gaataataca ccatgtggga gtacaacggc
     1561 catggtacct tcatcgtcta ggtaatgtac catgtgttga ataaaggcaa agtctgcttt
     1621 agacttaggc gcaagtttgc cgtaaccact gaatcgttcg tcattttcaa actttgaatc
     1681 tgcagtccat ttcgcactat acggtgggtt cgcaataacc gcatcaaatg tattgcctaa
     1741 aaaggctggg ttttccaatg tgtcatcatt acggatatcg aagttctcat aacgcacatc
     1801 atgtaataac atattcatgc gtgctaagtt gtatgtagta ttgttacgtt cttgaccgaa
     1861 ataacgatac acttgtgttt ctttaccaac acgtaacaac agtgaacctg aaccacatgt
     1921 tgggtcatac acgtgacgta atttatcttt accgtctgtg acaatcttcg ccagtatctt
     1981 agatacttgt tgtggtgtat agaactcgcc tgctttttta cc
//
LOCUS       DANJFZ010000213         1997 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.220, whole genome shotgun sequence.
ACCESSION   DANJFZ010000213 DANJFZ010000000
VERSION     DANJFZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.220"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(287..1933)
                     /locus_tag="PWN45_002795"
     CDS             complement(287..1933)
                     /locus_tag="PWN45_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL0624485.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          721 a          387 c          290 g          599 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaatt cataattaat tactcccttc aattctatat ttaattttat gtagtagaat
     1981 aaaaatatta tctaaac
//
LOCUS       DANJFZ010000214         1939 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.221, whole genome shotgun sequence.
ACCESSION   DANJFZ010000214 DANJFZ010000000
VERSION     DANJFZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.221"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..136
                     /locus_tag="PWN45_002796"
     CDS             <1..136
                     /locus_tag="PWN45_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDL0624486.1"
                     /translation="LEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN
                     "
     gene            255..581
                     /locus_tag="PWN45_002797"
     CDS             255..581
                     /locus_tag="PWN45_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDL0624487.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            583..1062
                     /gene="rsbW"
                     /locus_tag="PWN45_002798"
     CDS             583..1062
                     /gene="rsbW"
                     /locus_tag="PWN45_002798"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDL0624488.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            1037..1807
                     /gene="sigB"
                     /locus_tag="PWN45_002799"
     CDS             1037..1807
                     /gene="sigB"
                     /locus_tag="PWN45_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDL0624489.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
BASE COUNT          752 a          231 c          370 g          586 t
ORIGIN      
        1 tttagaatat attaaaaaac ataaacatat gcacccacaa gatattgttc aaattatcta
       61 tgaagcaatt ttaaagcttc aaaacccaaa taaaaaagat gatatgacta ttttgattat
      121 aaaaagagta aattaattta aaaaagaaga ttagaaatta tttcgatggg tatataataa
      181 tttgaaatat aaatatggtg gatacagcgc ttaaaatgaa gataaatatt tttaataagt
      241 aggagtgtaa tgaaatgaat cttaatatag aaacaaccac tcaagataaa ttttacgaag
      301 ttaaagtcgg tggagaatta gatgtttata ctgtgcctga attagaagag gttttaacac
      361 ctatgagaca agatggaact cgtgatattt atgttaattt agaaaatgtg agttatatgg
      421 attcgacagg tttaggttta ttcgtaggta cattaaaagc attaaaccaa aatgataaag
      481 aactatacat tttaggtgtg tcagatcgta tcggtagact atttgaaatt actggtctta
      541 aggatttaat gcatgttaat gaaggaacgg aggtcgaata acatgcaatc taaagaagat
      601 tttatcgaaa tgcgcgtgcc agcatcggca gagtatgtaa gtttaattcg tttaacactt
      661 tctggcgttt tttcgagagc tggtgctaca tatgatgata ttgaagatgc caagattgca
      721 gttagtgaag ctgtgacaaa tgcagttaaa catgcataca aagaaaataa caatgtgggc
      781 attattaaca tatattttga aattttagaa gataaaatta aaattgttat ttctgataaa
      841 ggtgacagtt ttgattatga aacaactaaa tcaaaaatag gtccttacga taaagacgaa
      901 aatatagact ttttacgcga aggtggccta ggtttatttt taatcgaatc tttaatggat
      961 gaagtcacag tatataaaga atctggtgtg acaatcagta tgactaagta tataaaaaaa
     1021 gagcaggtgc gaaataatgg cgaaagagtc gaaatcagct aatgaaattt cacctgagca
     1081 aattaaccaa tggattaaag aacaccaaga aaataagaat acagatgcac aggataagtt
     1141 agttaaacat taccaaaaac taattgagtc attggcatat aaatattcta aaggacaatc
     1201 acatcacgaa gatttagttc aagttggtat ggttggttta ataggtgcca taaatagatt
     1261 cgatatgtcc tttgaacgga agtttgaagc ctttttagta cctactgtaa tcggtgaaat
     1321 caaaagatat ctacgagata aaacttggag tgtacatgtt ccgagacgta ttaaagaaat
     1381 tgggccaaga atcaaaaaag tgagcgatga actaaccgct gaattagagc gttcaccttc
     1441 tatcagtgaa atagctgatc gattagaagt ctcagaagaa gaagtgttag aagcaatgga
     1501 aatgggacaa agttataatg cgttaagtgt tgatcattcc attgaagctg ataaagatgg
     1561 ttcaactgtt acgctattag atattatggg gcaacaagat gaccattatg acttaactga
     1621 aaaacgtatg attttagaaa aaatattacc tatattatct gatcgcgaac gagaaatcat
     1681 acaatgtacg tttattgaag gattgagtca aaaagagaca ggtgagcgta tcggtttaag
     1741 tcaaatgcat gtatcacgac ttcagagaac ggcaattaag aaattacaag aagcagcaca
     1801 tcaatagaat ttgtttatta atgatacgtt ttataatgaa aaatccatat aattatccct
     1861 tgattattaa attgaaatcg aggggtattt ttaatttaat taagattttc gaattaatac
     1921 attattagtg tagtttaat
//
LOCUS       DANJFZ010000215         1878 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.222, whole genome shotgun sequence.
ACCESSION   DANJFZ010000215 DANJFZ010000000
VERSION     DANJFZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.222"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            216..1454
                     /gene="pgaC"
                     /locus_tag="PWN45_002800"
     CDS             216..1454
                     /gene="pgaC"
                     /locus_tag="PWN45_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDL0624490.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            1418..1723
                     /gene="icaD"
                     /locus_tag="PWN45_002801"
     CDS             1418..1723
                     /gene="icaD"
                     /locus_tag="PWN45_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDL0624491.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            1720..>1878
                     /locus_tag="PWN45_002802"
     CDS             1720..>1878
                     /locus_tag="PWN45_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDL0624492.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRV"
BASE COUNT          634 a          285 c          328 g          631 t
ORIGIN      
        1 accccttctc tgaaaataag gttattgcgt tatcaataat cttatccttc aatttttata
       61 accccctact gaaaattaat cacactatgt tacaggaaaa ttaagttgca attacaaata
      121 tttccgttta attataacaa caatctattg caaattaaaa tactatcaat taccatatgg
      181 cttacaacct aactaacgaa aggtaggtaa agaaattgca attttttaac tttttgcttt
      241 tttatcctgt atttatgtct atttactgga ttgtcggttc aatttatttc tattttacca
      301 gagaaattag atattcattg aacaagaagc ctgacataaa tgtggatgaa ttagaaggca
      361 ttacattttt acttgcctgt tataacgaaa gtgaaacgat tgaagatacg ttgtctaatg
      421 ttcttgcact caaatacgag aagaaagaaa ttattatcat taatgatgga agttcagata
      481 atacagcaga actcatctat aaaatcaaag aaaataatga ctttattttc gtcgatttac
      541 aagaaaacag aggtaaagcc aacgcactca atcaaggcat taaacaggct tcatatgatt
      601 atgtaatgtg cttggatgca gatactatcg ttgatcaaga tgcaccatat tatatgattg
      661 agaatttcaa acatgatcca aaacttggtg cagttacagg taatcctaga attcgaaata
      721 agagttctat tttaggtaaa attcaaacga tagaatatgc aagtttaatt ggctgtatta
      781 agcgaagtca gacacttgct ggcgcagtca atactatttc gggtgtcttc actctattta
      841 aaaaaagtgc agttgtcgac gttggctact gggatactga tatgattacc gaagatattg
      901 cagtttcttg gaaattgcat ttacgtggat atcgtattaa gtatgaaccg cttgccatgt
      961 gttggatgtt ggttccagaa acattgggag gtctttggaa gcaacgcgtg agatgggctc
     1021 aagggggaca cgaagtatta ctacgagact tttttagcac aatgaaaacg aaaaggtttc
     1081 ctttatatat tttgatgttt gagcaaatca tctcgatttt atgggtatat atagtgcttc
     1141 tatatttagg ctatttgttc ataacagcaa acttcttaga ctatacattt atgacatata
     1201 gtttttcaat atttctacta tcatcattta ctatgacttt tataaacgtt attcaattta
     1261 cagtcgcact ctttattgat agtcgctacg agaaaaagaa tatggctgga ctcatatttg
     1321 taagttggta tccgacagta tactggatta ttaacgcagc agtagttctt gtcgcatttc
     1381 caaaagcatt aaaacgtaag aaaggtggtt acgcaacatg gtcaagccca gacagaggga
     1441 atacccaacg ctaaaatcat cgctaaatat tgtaagagaa acagcactta tcgctatatc
     1501 ttgtgtcttt tggatatatt gtttagttgt tctactcgtt tatattggta ctatatttga
     1561 aattcatgac gaaagtatca atacaatacg tgttgcttta aacattgaaa atactgaaat
     1621 tttagatata tttgaaacta tgggcatttt cgcgattatc atttttgtat tttttacaat
     1681 tagcatattg attcaaaaat ggcagagagg aagagaatcg tgaagtatag aaaatttata
     1741 attttagtgt tgagtatctt gatcatattg cctgtaagca cactggatgg tcatcatatt
     1801 gcaaatgcag atgacgattc acctaaaaaa ctgaaatata aagaaaatag tgctctggca
     1861 ttaaattatc accgtgta
//
LOCUS       DANJFZ010000216         1862 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.223, whole genome shotgun sequence.
ACCESSION   DANJFZ010000216 DANJFZ010000000
VERSION     DANJFZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.223"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            184..1674
                     /locus_tag="PWN45_002803"
     CDS             184..1674
                     /locus_tag="PWN45_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL0624493.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          750 a          183 c          369 g          560 t
ORIGIN      
        1 cgtgaaataa tcagaggaat cgtttgaaat catcgtagta atcgcgcgaa aatcgcgtga
       61 aataatcaga ggaatcgttt gaaatcatcg tagtaatcgc gcgaaaatcg cgtgaaatac
      121 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
      181 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      241 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      301 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      361 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      421 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      481 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      541 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      601 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      661 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      721 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      781 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      841 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      901 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      961 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
     1021 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
     1081 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1141 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1201 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1261 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1321 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1381 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1441 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1501 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1561 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1621 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1681 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1741 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1801 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1861 tt
//
LOCUS       DANJFZ010000217         1770 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.224, whole genome shotgun sequence.
ACCESSION   DANJFZ010000217 DANJFZ010000000
VERSION     DANJFZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.224"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            93..194
                     /locus_tag="PWN45_002804"
     CDS             93..194
                     /locus_tag="PWN45_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624494.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            204..476
                     /locus_tag="PWN45_002805"
     CDS             204..476
                     /locus_tag="PWN45_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDL0624495.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            520..>1770
                     /locus_tag="PWN45_002806"
     CDS             520..>1770
                     /locus_tag="PWN45_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDL0624496.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVE"
BASE COUNT          692 a          216 c          274 g          588 t
ORIGIN      
        1 taggcgacat gttcctgcat atcgtctatt tatacactgc acaatttaat aataatacaa
       61 ttatactttg atgtccattt aacataaagt ggttgaaatt tgcagcaaat aaattaacct
      121 tgactatttt cagaaaattc tgtataaata acgaaaaaga gatatggatt aaatatgaag
      181 aggtgttttt atagaagaat aaaatttttg gcactatatt atatttaact ttagcacttg
      241 gattatcaac agcagcttat gcatctacag aatacgcaga aggaggcact tggagtcatg
      301 gtgtcggcag taagtatgtt tggtcgtatt attatcatgg tcataaagga catggtgcaa
      361 cagctattgg aaaatataga tcatttagtg gttatacaag agctggtgta aaagcaaaag
      421 catcagctac taaacataat tggtgggtca atagagcgta ttataacatt tattaaatac
      481 taaaaaatca tagaaaataa acaacgcaag aggtacaaca tgaaatggtt caaattaata
      541 cttgatgtta caatcttcat tctgattgct atattgttat ttgtttatac atataaagaa
      601 aacgaagaaa tattgcctga tactaaatac cctatagcgg taactgactg gaataaaaaa
      661 tatagtaaaa atgagattta taaacgtata gatcaattcg ctaaaaatga gaacgtagca
      721 atctataaat caacttcaaa ttatacaaac aaaaacgtag ataaagatat atatgtattt
      781 aataaatcaa aagcagcaac tatcactcct tttaatgcta aatataacat tcattattta
      841 agtgacgacg aagtattaaa aaaagatatc aaagggagtt attttgtaaa agacaaaaat
      901 tttgacgtgt ctaaattcat aaatttttta aaagaatatg gtgttactgc tgaatcatac
      961 aaaatagatc atatgatgat tgccgttggt gtcattaaac aaatgaatat agtagttcca
     1021 ttatcctcac ttttaatcgt ttattttatt tattatattt tcgaaaagaa cattaatttc
     1081 aaagcgtatg cgattaagta tttaaatggt tttacattaa gaaaaataat tttcgaaaat
     1141 ttttcaaaga aatgcacgta ttgggtaacg ttaatcataa ctcaaattct tttaactaca
     1201 agtgtacttt ggatattaaa ttacacgggt aatttagatt tattcatatt aagaatagtt
     1261 ctgctttcat gtcttttcat tttaacgatt agtgttatta atttatggac tttcttaatg
     1321 ttactaaatt taaatattgc aaatatgatt aaaggtaagc agcactttaa aacaattcgt
     1381 tttattaata cagtttgtaa aagtattctc ttagtactaa tagctaatgt aatgatagaa
     1441 aatactagtg ttattaaaga tttgaataaa ataaaagaaa ctgagaaata ttggaatgta
     1501 ttagatgatt attacacgat tgaatttgca ccttatcacg aaacaaaaca aagtttgatt
     1561 gataatatgg tgcgatcaga acaattagta aaggctagtg aagcagaaaa taatgcgatt
     1621 ttattcaaac caaagggtga ctccgttgac aatgacaact tttcgcctga tgaggggaat
     1681 gtaatattag taaataatca gttctggtcg atttattaca agcagtttca acctgatatt
     1741 ccgataaaaa atcaaaaaaa taatgtcgaa
//
LOCUS       DANJFZ010000218         1726 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.225, whole genome shotgun sequence.
ACCESSION   DANJFZ010000218 DANJFZ010000000
VERSION     DANJFZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.225"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..199
                     /locus_tag="PWN45_002807"
     CDS             <1..199
                     /locus_tag="PWN45_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL0624497.1"
                     /translation="LLHGLIIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTN
                     FVCKWTNPIINLQRPSQFKDS"
     gene            395..1549
                     /locus_tag="PWN45_002808"
     CDS             395..1549
                     /locus_tag="PWN45_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0624498.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          607 a          259 c          254 g          606 t
ORIGIN      
        1 tttattacat ggtttaatta ttggcattgt tagaggattt gaatggtacc cattcgataa
       61 tcctatatca ttaatgactt atctttattt aataagtata tctgtaatca tcgtatatgt
      121 attatctaca aactttgttt gtaaatggac aaatccaata attaatttac aaagaccatc
      181 acagtttaaa gattcataat tttatactgt ctaagtacat taactgtaac cactttattc
      241 attttgaaaa gtagttacta cagtgtattt agacattttt cgtttttcaa ataaagctta
      301 agtaatttac ccttttttgc tttaaaagta tgttatgata ttattaattt ctaaattttt
      361 agacaatatt tgatttatat ttgaggtgtc acaaatgaaa ctttctttaa attctaattc
      421 taaatattta agagcaccaa gtattcgcca attttcaaat cgtatgaata atttggatga
      481 ttgtgttaat ttaactattg gccaaccaga cttcccgatg cctgatgttg tgaaaaaggc
      541 atatattgat gctattaaca atgacaagac atcatactct cacaataaag ggttattaga
      601 aactcgcgaa gcaattagtc aatacttcaa aaatcgttat catttttcct atgatcctga
      661 agaaattatt gtgacaaatg gagcaagtga agcaatagac acaacgttac gttctatcat
      721 agagcctgga gatgaaatta taataccggg accaatttat gcaggctaca taccactcat
      781 cgaagtacta ggtggtaaac caatttatat tgatacaaca gcaacacaat ttaaaattac
      841 acctgatgca ttagaaagtc atatttctcc aaagacaaaa gctgtcttgt taaattatcc
      901 gactaatcca actggcgtag ttttaaaaag aaatgaagtt ttaaatatcg taaatgtatt
      961 aaaaaaatat ccgttattta ttattagcga tgagatttat gctgaaaata catttagtgg
     1021 taaacatgta tccttcgctg aatttgaaga cattcgtgat caattaatat taattggtgg
     1081 tttaagtaaa tcacactcag caaccggaat aagaattggt tttctattag gacctcaata
     1141 tttgattgat aaattaacat ttatgcacgc ctataattgt atttgtgcca atgttccagc
     1201 tcaaatagca tgtataacag cacttaacga aggattagaa gctcctaaat atatgaacga
     1261 agcttatgta gaacgaagaa attatttagt atctgaatta actaaattag gttttgagat
     1321 taccgctcaa cctgaaggcg cgttttatat tttcccaagt attaaacata tcaccgatga
     1381 cgatttcgaa ttttgtgtcg atttacttga atcaacacat ttagcaatcg ttccaggctc
     1441 atcctttaca gaattcggta aaggatttgt aaggatttcc tatgcttatg aaatggatgt
     1501 tttaaaagaa ggtatgaaga gacttgctaa atatttaaat actaaataaa aatgatatgt
     1561 tgaactggac gtcgtgaact aaattcatag tacgttcagt tttttatatt ctataagttt
     1621 taaataatac tctatagaga ctgtaaatac taccgagtta tattcctctc ctttaaacac
     1681 ctcactcaaa atgattctat caaaaggttt taatatgcta acttat
//
LOCUS       DANJFZ010000219         1696 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.226, whole genome shotgun sequence.
ACCESSION   DANJFZ010000219 DANJFZ010000000
VERSION     DANJFZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.226"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            93..1259
                     /gene="scpA"
                     /locus_tag="PWN45_002809"
     CDS             93..1259
                     /gene="scpA"
                     /locus_tag="PWN45_002809"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDL0624499.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1290..1616
                     /locus_tag="PWN45_002810"
     CDS             1290..1616
                     /locus_tag="PWN45_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDL0624500.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
BASE COUNT          673 a          235 c          250 g          538 t
ORIGIN      
        1 aattattttt taaacataga ttaaaatttt atattgaaac tcttaactaa atttaatata
       61 attattttat aataaaataa aggagttttt atatgaaaag aaactttcca aaattaatag
      121 ctttaagttt aatattttct ttaagtgtta cccctattgc aaacgctgag agcaattcaa
      181 atatcaaagc aaaagataag aagcatgtgc aagttaatgt cgaggacaag agtgttccta
      241 ctgacgtacg taatttagcg caaaaagact atctttctta tgttacttca cttgataaaa
      301 tctataataa agagaaagcc tcgtatacac ttggtgaacc ttttaaaatt tataaattca
      361 acaaaaagag tgatggtaat tattattttc cagttttgaa cactgaaggt aatatcgatt
      421 atatagttac aatttcacca aaaatcacta agtattctag ttcatcttct aaatacacaa
      481 tcaatgtttc tccattttta tctaaagtct taaatcaata taaagatcaa caaattacta
      541 ttttgacaaa ctcaaaaggt tactatgtgg ttactcaaaa tcataaagct aaactagtac
      601 taaaaacacc acgtctagaa gataaaaagt taaaaaagac tgagagtata cctactggca
      661 ataatgtaac gcaactaaag caaaaagctt ctgtcactat gccaactagt caatttaaaa
      721 gtaataatta tacttacaac gagcaatata taaataaact tgagaatttc aagataagag
      781 aaactcaagg taacaatggt tggtgtgcag gctatacgat gtctgaatta ctaaatgcga
      841 cttataatac taacaaatat catgcagaag ctgtgatgag atttttacac ccaaacttac
      901 aaggacaacg attccaattt actggtttaa cgccaagaga gatgatttat tttggacaaa
      961 ctcagggaag aagtccacaa cttcttaata gaatgactac atataatgaa gttgacaatc
     1021 ttactaaaaa taataaagga attgctgttt taggtagtag agtagaatca agaaatggta
     1081 tgcacgcagg gcatgccatg gcagttgttg ggaacgctaa attagataat ggacaagagg
     1141 ttataattat atggaatcca tgggataatg gttttatgac tcaagatgct aaaaacaatg
     1201 ttattccagt atccaatggc gaccattatc gatggtattc atctatttat ggttattaaa
     1261 atttgtattt tagaaaggta aataatatta tggaacaaat tgaattattt agtattgata
     1321 aattcaaatg taattcagag gctaagtatt atcttaatat tattgaggga gaatggcatc
     1381 ctcaagattt aaatgatagt cctttaaaat ttattctcag tacctcagac gattctgatt
     1441 acatttgcaa atatataaat acagaacaca aacaactcac attatataat aaaaataata
     1501 gctcaattgt tattgaaata tttataccaa atgataataa aatactacta acaattatga
     1561 acacagaagc tttgggaact tctcctagaa tgactttcat taagcataag tcataagact
     1621 atctagatat gattaagtaa actattaatc ttattaaagg tctctaggaa gaaaaaattt
     1681 catgcgatga gattgt
//
LOCUS       DANJFZ010000220         1696 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.227, whole genome shotgun sequence.
ACCESSION   DANJFZ010000220 DANJFZ010000000
VERSION     DANJFZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.227"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            239..>1696
                     /locus_tag="PWN45_002811"
     rRNA            239..>1696
                     /locus_tag="PWN45_002811"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          514 a          318 c          469 g          395 t
ORIGIN      
        1 atagctcagc tggttagagc gcacgcctga taagcgtgag gtcggtggtt cgagtccact
       61 taggcccacc attatttgta cattgaaaac tagataagta agtaaaatat agattttacc
      121 aagcaaaacc gagtgaataa agagttttaa ataagcttga attcataaga aataatcgct
      181 agtgttcgaa agaacactca caagattaat aacgcgtttc ctgtaggatg gaaacataga
      241 ttaagttatt aagggcgcac ggtggatgcc ttggcactag aagccgatga aggacgttac
      301 taacgacgat atgctttggg gagctgtaag taagctttga tccagagatt tccgaatggg
      361 gaaacccagc atgagttatg tcatgttatc gatatgtgaa tacatagcat atcagaaggc
      421 acacccggag aactgaaaca tcttagtacc cggaggaaga gaaagaaaat tcgattccct
      481 tagtagcggc gagcgaaacg ggaagagccc aaaccaacaa gcttgcttgt tggggttgta
      541 ggacactcta tacggagtta caaaggacga cattagacga atcatctgga aagatgaatc
      601 aaagaaggta ataatcctgt agtcgaaaat gttgtctctc ttgagtggat cctgagtacg
      661 acggagcacg tgaaattccg tcggaatctg ggaggaccat ctcctaaggc taaatactct
      721 ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cggaagggga
      781 gtgaaataga acctgaaacc gtgtgcttac aagtagtcag agcccgttaa tgggtgatgg
      841 cgtgcctttt gtagaatgaa ccggcgagtt acgatttgat gcaaggttaa gcagtaaatg
      901 tggagccgta gcgaaagcga gtctgaatag ggcgtttagt atttggtcgt agacccgaaa
      961 ccaggtgatc tacccttggt caggttgaag ttcaggtaac actgaatgga ggaccgaacc
     1021 gacttacgtt gaaaagtgag cggatgaact gagggtagcg gagaaattcc aatcgaacct
     1081 ggagatagct ggttctctcc gaaatagctt tagggctagc ctcaagtgat gattattgga
     1141 ggtagagcac tgtttggacg aggggcccct ctcgggttac cgaattcaga caaactccga
     1201 atgccaatta atttaacttg ggagtcagaa catgggtgat aaggtccgtg ttcgaaaggg
     1261 aaacagccca gaccaccagc taaggtccca aaatatatgt taagtggaaa aggatgtggc
     1321 gttgcccaga caactaggat gttggcttag aagcagccat catttaaaga gtgcgtaata
     1381 gctcactagt cgagtgacac tgcgccgaaa atgtaccggg gctaaacata ttaccgaagc
     1441 tgtggattgt cctttggaca atggtaggag agcgttctaa gggcgttgaa gcatgatcgt
     1501 aaggacatgt ggagcgctta gaagtgagaa tgccggtgtg agtagcgaaa gacgggtgag
     1561 aatcccgtcc accgattgac taaggtttcc agaggaaggc tcgtccgctc tgggttagtc
     1621 gggtcctaag ctgaggccga caggcgtagg cgatggataa caggttgata ttcctgtacc
     1681 acctataatc gtttta
//
LOCUS       DANJFZ010000221         1687 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.228, whole genome shotgun sequence.
ACCESSION   DANJFZ010000221 DANJFZ010000000
VERSION     DANJFZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.228"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            18..197
                     /locus_tag="PWN45_002812"
     CDS             18..197
                     /locus_tag="PWN45_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624501.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            508..768
                     /locus_tag="PWN45_002813"
     CDS             508..768
                     /locus_tag="PWN45_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624502.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(821..1171)
                     /gene="scn"
                     /locus_tag="PWN45_002814"
     CDS             complement(821..1171)
                     /gene="scn"
                     /locus_tag="PWN45_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDL0624503.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          578 a          222 c          220 g          667 t
ORIGIN      
        1 aaggaagtaa taaaaacgtg aaatttaaaa caactaaaga atgtaaaagt aataatatct
       61 ttaaaagaag tcaagaaatt aataatagag aaagtgaaaa gggttgttta tggggcatta
      121 gcatgttgat tctacttttc ttattgattc tgtttggaat aactgcttgt tcatcaagca
      181 ttcactttat taattagatt tttttacttg gaggtatcat gtgaagaacc atacaaatat
      241 aattaatatc ttattagtta tagtcaactc attaactcat tttctaactc taaacacctc
      301 attttttaat aattcagcat cggatttctg ttttatcata ggggctatat ttttcttaat
      361 cggaattttt gttgcaatat acggtatgaa gcgagcaaca tattggttaa acttattgat
      421 tttatttacc aatatttttt attttctaca cttctgtgtg ttacttttgt taaaatatat
      481 aggatttaaa ttatttattt atgaagggtg tgtattgtta tttatctaat ttatagtcta
      541 attgtctatt tcatctgcat tataaatttt ttcataatgg ctgcaccatt cttaatgact
      601 aatgctgatt atgtatggac gcctatgact acagttacgt tatttatttt gagtttgatt
      661 attttcttga tatttataaa aacaaaagat gtcgttcatt taacaatttt catattaaac
      721 ttactttttt caatacttta ttgtttgcct atactgtttt atttatgaac acttttatat
      781 tatctagaaa attaaaacca cccgtaaaag ggtggttttt ttaatattta ctttttagtg
      841 cttcgtcaat ttcgttataa atcttttgaa gttgatattt tgcttctgac attttcttaa
      901 agtcttttga cttaagagca tacattgctt tttgacctga aatttttata gttcgcttat
      961 aataagtgtt taaacttcca gtagctaatt cattaacatt tagttcatct aataacgatt
     1021 ttaattcatt agctaacttt tcgttttgat attcattcga tgttggcaag cttgtgctag
     1081 cttgtgcctc atttttatct agattagtta ctagtggtga tgctaaaacg attgctaaag
     1141 ttcccgcaag tatagatttt ctaattttca ttttttattc tccttagtaa ttagtactaa
     1201 tttattttaa tgactgacta aaaaaaagta cattaattat tcattaacta tatagatctt
     1261 cttattaaat gaaaattaat aaattatcaa attgacatac cttattgtat ttatagaaaa
     1321 acaaaaaaag gtaagcaccg aaatgcttac ctacttccca taaacaatat aacacatata
     1381 cattgatttg gaaagcgcaa aaataaattt aatatactag cccgaagggg agcaatacat
     1441 aaaaaatgaa aggcgctcct tgaaaacgcc caaggtaata ttaacataaa atggctacta
     1501 ttgcattatc taattttatt taattaaaac aaatatatat agcacaaaaa aactagcccg
     1561 aaggctagct tatgaataga tgaaaatttg aacacattgc tgtgtctaag ataaatatag
     1621 cacatattaa ttactattaa tagaaaaatt atagcgtttt ttagattatt tcaatttatc
     1681 gagacca
//
LOCUS       DANJFZ010000222         1686 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.229, whole genome shotgun sequence.
ACCESSION   DANJFZ010000222 DANJFZ010000000
VERSION     DANJFZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.229"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..445
                     /locus_tag="PWN45_002815"
     CDS             <1..445
                     /locus_tag="PWN45_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0624504.1"
                     /translation="IVLTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYV
                     FRKVIFGELFNAIKGNRIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVW
                     TKRLAVPIIIHMLQNGFVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(470..>1686)
                     /locus_tag="PWN45_002816"
     CDS             complement(470..>1686)
                     /locus_tag="PWN45_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624505.1"
                     /translation="SILILSSSSLGLATHTVEAKDNLNGEKPTTNLNHNITSPSVNSE
                     MNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPSTDSKPDPNNQNPSP
                     NPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDPNKPNPDPKPDPDK
                     PKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGN
                     QGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQN
                     FSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDK
                     PDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKR
                     NKKKNKNESQRR"
BASE COUNT          436 a          273 c          312 g          665 t
ORIGIN      
        1 cattgtatta acgcaaattt atggtggaca acaagtaagt cctaatacag aaaagctaat
       61 tattattgct cgaaaaatac ctatatttat cttctttgta tctattattg gtcctttatt
      121 agaagaatat gtattcagaa aagtaatctt tggagaatta tttaatgcga ttaaaggtaa
      181 tcgtatcgtg gcatttatta ttgctacaac agtaagttca ttaatatttg cattagcaca
      241 taatgatttc aaatttattc cagtttattt tggtatgggt gtcatttttt cattagcata
      301 tgtttggaca aaacggcttg ctgttccaat tattatccat atgttacaaa acggatttgt
      361 cgttatattc caattactta atcctgaggc tttgaaaaaa gccacggaac aagcgaattt
      421 tatatatcac atttttattc cataataaaa aaaacgaatc acacacggat tatcgtcgct
      481 gtgattcgtt tttatttttc tttttatttc gctttacaaa taagaatagc atgattgaaa
      541 ataccaccat catagaacat acaattgcta gccaactttt actatatgca gttgacgcac
      601 ttttcttatt atcttcttgt tttttaacta ctgtaaaatc gtctttttca tgcggttcaa
      661 atgagcgttg ttttgaatcc ggcttatctt cgggcttttt caaatctggc attgatccaa
      721 tttcaccagt aattaatgcg agtacttgtt catctaaatt acgatagtac ctttctgatt
      781 tcaatggatt gaaatattgg aatctaaagt aattcgattg ctgttgtaat ccatttaaat
      841 atgcatttcc gctgaaattt tgttttcgaa taatattata aatgtcttca tcagtaactt
      901 ctccataatc tttgcccaac ttattaattt gatttaaaat atacggatta tacttgtaag
      961 ccccatttgc caaggctaaa aatcgttggg atacaaaatc attaccagta ggattttgtg
     1021 agtttccttg attcccattt ggttgccatt gaccttgatt agatccatct gaagcatttg
     1081 ggttccatgt ccccccattt ttcgagccac cagaatgatt ggaatcccca ggttgatcgg
     1141 gatctggtga cgggttagga tttggcttat tagggtctgg ttttggattc ggatttggct
     1201 ttggtttatc tggatctggt tttggatccg gatttggctt attagggtct ggttttggat
     1261 ctggatttgg tttcgggtta tctggatctg gttttggatc cggatttggc tttggtttat
     1321 ctgggtctgg ttttggatcc ggttttggtt tcgggttatc tggatctggt ttaggattcg
     1381 gacttgggtt ttggttattt gggtctggtt ttgaatctgt acttgggttt tggttgtttg
     1441 agtctggctt cacattattc gaatcaggat tagcatcacg actattcgaa cctgttcctt
     1501 cattacccgt ttgatttgat tcgtgaggtg tcccagtctc attattattc atttcactat
     1561 ttactgatgg tgaagttata ttatgattca aattagtagt tggtttttct ccatttaagt
     1621 tatcctttgc ttcaactgtg tgcgttgcaa gccctaaact actactactt aaaattaaaa
     1681 tacttg
//
LOCUS       DANJFZ010000223         1645 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.230, whole genome shotgun sequence.
ACCESSION   DANJFZ010000223 DANJFZ010000000
VERSION     DANJFZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.230"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..488
                     /locus_tag="PWN45_002817"
     CDS             <1..488
                     /locus_tag="PWN45_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL0624506.1"
                     /translation="NNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITL
                     QSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRG
                     KLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSI
                     K"
     gene            502..813
                     /locus_tag="PWN45_002818"
     CDS             502..813
                     /locus_tag="PWN45_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL0624507.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            791..1237
                     /locus_tag="PWN45_002819"
     CDS             791..1237
                     /locus_tag="PWN45_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL0624508.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            1224..1523
                     /locus_tag="PWN45_002820"
     CDS             1224..1523
                     /locus_tag="PWN45_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDL0624509.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            1441..>1645
                     /locus_tag="PWN45_002821"
     CDS             1441..>1645
                     /locus_tag="PWN45_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL0624510.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDL"
BASE COUNT          647 a          189 c          260 g          549 t
ORIGIN      
        1 ataacaattt aaatatgctc aataagataa actttgtgat gaaactaccg ctaatatcag
       61 gctatttcca attatttaaa acttattttg taactaatga attagtgttg ttttataaaa
      121 atggtattac acttcaatca atagtagacg tttatattaa ccatagtagt gatccattta
      181 gacagtttct aggtaaatac ttattaactt attcagaaat gggatatggt ttgcctcaaa
      241 ttttagaaaa actaaaatgc tttaagcctc aattaattaa gtttgtgcta caaggtgaaa
      301 agagagggaa gctagaagta gaactaaagt tatattcgca aatattagta aaacaaatag
      361 aagataaagc gataaaacag actcagtttt tacagcctat tttatttttg attttaggtt
      421 tatttattgt cgcaatttat ttagtaatta tgttaccaat gtttcaaatg atgcaaagta
      481 taaaataatg gaggttaata tatgtttaaa tttcttaaga aaactcaagc gtttacattg
      541 atagagatgc tattagtgtt attaatcatc agtttattat taattttaat cattccaaat
      601 attgctaaac aaactgctca catacaatca acaggttgta atgcacaggt aaaaatggtt
      661 aatagtcaaa ttgaagcgta tgcattgaaa cataatagaa atccatcgtc tattgaagac
      721 ttaattgcag atggttttat aaaagaagca caaaagacat gtaaatcagg agagacaatc
      781 acaattagta atggagaagc agttgcaaat tagaaagcag tcagcattta ctatgattga
      841 gatgcttgtg gtaatgatgt taatcagtat atttctactt ttgacaatga catctaaagg
      901 attaagcaat cttagagtaa tagatgatga ggcaaatatc atttctttta ttactgaatt
      961 gaattatatt aagtcgcaag ctatagcaaa tcaaggatat atcaatgtta gattttatga
     1021 aaacagtgac actattaaag taatagagaa taataatata cgatttctaa aattaaaagt
     1081 aggcaaaata attaatgttg caaaagttga tattattgcc tttgataaaa aagggaatat
     1141 caataaattt ggtagcataa caatttacaa taacaattca atttatagaa taatattcca
     1201 tattgaaaaa ggaagaattc gttatgaaaa gctataagtg taaaggttca ttcttaatag
     1261 atagtatggc tggatttttg ctaattggat tgattacatt actattgata ccaatgatga
     1321 atcaaatgca agcgagtata aaccataaac tacaaacaat tgatgcttct aaagtaattt
     1381 tgacgactgt atctaaaatt aataaagaag aacttaagaa gggggtaact atagggaagt
     1441 atgatattaa gcaaagtgac caacaaattt gtgctatttc aaaaaatacc acttcttatc
     1501 aaaagacatg tatacagtat taatgtcaaa gctttttcgc tcattgaaat gttagtagcg
     1561 atgatggtta taagtataac tttactaatt gttccagact taattagact tagtaaaact
     1621 tttctaattg aaagtaggga tttaa
//
LOCUS       DANJFZ010000224         1617 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.231, whole genome shotgun sequence.
ACCESSION   DANJFZ010000224 DANJFZ010000000
VERSION     DANJFZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.231"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..1150
                     /gene="fmhC"
                     /locus_tag="PWN45_002822"
     CDS             <1..1150
                     /gene="fmhC"
                     /locus_tag="PWN45_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDL0624511.1"
                     /translation="NKINHEAHIVGVKNDKNEVIAACLLTEARIFKFYKYFYSHRGPL
                     LDYFDAKLVCYFFKELSKFIYKNRGVFILVDPYLIENLRDANGRIIKNYNNSVIVKML
                     GKIGYLHQGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKV
                     EDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDK
                     LKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKTEELIFEDGP
                     VLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGIT
                     GVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV"
     gene            1323..>1617
                     /locus_tag="PWN45_002823"
     CDS             1323..>1617
                     /locus_tag="PWN45_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL0624512.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFP"
BASE COUNT          657 a          159 c          253 g          548 t
ORIGIN      
        1 aaataaaata aatcatgaag cacatattgt gggagtgaag aatgataaaa atgaagttat
       61 agctgcatgt ttattaacag aggcacgaat ttttaaattc tacaaatatt tctactctca
      121 tagaggtcct ttacttgatt atttcgatgc taaattagtt tgttactttt ttaaagaatt
      181 atctaaattc atttataaaa atagaggagt atttattctt gttgatccat atttaataga
      241 gaatttaaga gatgcaaatg gtaggataat aaagaattat aataattcag tgatagtaaa
      301 gatgctaggg aaaattgggt atctccatca aggttataca acaggatatt caaataaaag
      361 tcaaattagg tggatttctg tattggattt aaaagataaa gatgagaatc aacttttaaa
      421 agaaatggaa taccaaacta gaagaaatat aaaaaagact attgagattg gtgttaaggt
      481 tgaagattta tctattgaag aaacaaatcg attttataaa ttgtttcaaa tggctgaaga
      541 aaaacatggt tttcatttca tgaatgaaga ttattttaaa cgaatgcaag aaatatataa
      601 agataaggca atgttaaaga tagcttgtat aaatcttaat gaatatcaag ataaattaaa
      661 aatacaatta ttgaaaatcg aaaatgaaat gatgactgtg aacagagcat taaatgaaaa
      721 tccaaattct aaaaaaaata aatcaaaatt aaatcagtta aatatgcaat tatctagtat
      781 taataataga attagtaaaa ccgaagaact aatatttgaa gatggacctg ttttggattt
      841 agctgctgct ttatttatat gtactgatga tgaagtttat tatctatcaa gtggatcaaa
      901 tccgaaatat aatcagtata tgggtgcata tcatctacaa tggcatatga taaaatatgc
      961 aaaatcacat aatattaata ggtataattt ttatggaata acaggcgtct ttagtaatga
     1021 ggcggatgat tttggtgttc aacaatttaa aaagggtttt aatgcacatg ttgaagaatt
     1081 aattggtgat ttcatcaaac cagtaagacc aattctatat aaatttgcaa aacttattta
     1141 taaggtttaa ttataaagta tgttggaaat tgaaatttta aattctttcc aacatacttt
     1201 tcacttttta agtcagtatt tttactcctt aaaataacat tacatcgaca atgtttttgt
     1261 attagaaatg taaaacaaaa atagaatatg attttgtatg attacaaaaa ggagttgatt
     1321 tattgattaa actatttttg cttaagttat actgggcaca cttttcgact aaacaaattc
     1381 atcaattttt aatggcatat cctaatgtaa ttaaagagga gggaagaaaa aaagatagtt
     1441 atttatgtga atgggtgaat agggaagaaa atgttcattt attacgtaaa tactatgctt
     1501 ttataaaact tgatcataac gatattatta aagaactgca gaaattaaaa gtaagttaca
     1561 ttacatatat ggattctgaa tacccagtgc tattaaaaga aatatatcaa tttccat
//
LOCUS       DANJFZ010000225         1572 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.232, whole genome shotgun sequence.
ACCESSION   DANJFZ010000225 DANJFZ010000000
VERSION     DANJFZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.232"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            390..575
                     /locus_tag="PWN45_002824"
     CDS             390..575
                     /locus_tag="PWN45_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624513.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            1087..1191
                     /locus_tag="PWN45_002825"
     CDS             1087..1191
                     /locus_tag="PWN45_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624514.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            1320..1571
                     /locus_tag="PWN45_002826"
     CDS             1320..1571
                     /locus_tag="PWN45_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624515.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
BASE COUNT          662 a          163 c          248 g          499 t
ORIGIN      
        1 attaaattct aaagatatct caatatctca aatttaatag actactggag ttagtagttt
       61 gaatattgaa agacactaat ctaataaaaa tacataaaag gttaaacttt agaagtggct
      121 gtaaacttca atgaatacta aacattgaag ttttgtaaaa ggcgtaaggt atacaagaca
      181 aatcgtttat ctttgacaaa gtaattgatg atgaaaagtc gttaatatgt agaaacaaaa
      241 attgaaaata taaaatttaa ctattatgaa gtgatgatag ccattaatga atagaaaaat
      301 aataaaatca tttatagata aaaaatacgt taagttcaca gaatttcgaa aaaaagcaga
      361 aaacattgta cagatatgga gatgaaaaaa tgtcagaata caagaaaaaa ataattgaat
      421 taattgaaag taatttaaca ggatatgaaa tttctaaaaa aactggagtt tctcaatacg
      481 tactttcaca attaagacag ggcaaacgcg aagtagataa tctaaccctg aatacaacag
      541 aaaaattata tgaatatgcc aataaagttt tgtaatttaa ctaatgtata aattaatcaa
      601 gctatgttta tttgatttaa ctattaataa aaatcatatg gtgaatggat attataataa
      661 ttaaaataca aaaatagtag attccaattt gtgaaaaagg agtatgtcgt ttcatttaga
      721 atttattgca cgaaattagc atcgaataag ggaaaataag tgttaagttt taaatgataa
      781 aaaagattgg aatggatcgt cttgaaatgc tcccttcaaa gttttcattt tttcaatgtc
      841 gacttcgaag ggggcatttt cattaaattg ttatagcttt ttatatttgt ataatgaaca
      901 tataagttta agaaggtgcg agtgaaggaa ataaaaaagc tcaaatgtac caaattgtta
      961 atcttaataa atctctactt tataaagatt gaatggacat tcgagcgtta atcagtcagg
     1021 agggactttc cctcctacaa tttaataata atacttgctt caccactata caaggagtga
     1081 gttgttatgt tcaaagtgaa ttattcgatt ttaagttatt atccagaata taatatcgca
     1141 gtaagttggc aacgtttaag agaaggaaaa acaataaaaa acaagattta atactgctgc
     1201 gtcatgaggc gcttgaacat tatttgatga ataagtataa tttcaactat gattatgcac
     1261 ataaaattgt atcaaaaaaa tacgattatt caatttttat aaaaaagaag gtggattaaa
     1321 tgcttacatt aataaaattg aaagaagatg aacaggttat aatatatgaa tatatacctg
     1381 aagatgatat aagtaacggt aaaggttcag taacttttaa taaaaaagat gcagaggtta
     1441 tagatttctc attatctgaa atagaaaatg aagaatattt tatgttatat cgtaataagt
     1501 ctttttctgt agtaagagac tttatcgaga agcaagaatt tcccgaaaat tataaaatag
     1561 catggtatta aa
//
LOCUS       DANJFZ010000226         1562 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.234, whole genome shotgun sequence.
ACCESSION   DANJFZ010000226 DANJFZ010000000
VERSION     DANJFZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.234"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            103..451
                     /locus_tag="PWN45_002827"
                     /pseudo
     CDS             103..451
                     /locus_tag="PWN45_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="PWN45_002828"
     CDS             462..656
                     /locus_tag="PWN45_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624516.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            697..1047
                     /locus_tag="PWN45_002829"
     CDS             697..1047
                     /locus_tag="PWN45_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624517.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1054..1245
                     /locus_tag="PWN45_002830"
     CDS             1054..1245
                     /locus_tag="PWN45_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDL0624518.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
BASE COUNT          671 a          170 c          241 g          480 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcatcttta ttaccaagtt atactataat agattctaat
      841 gaaagtgaaa agatgactaa aaaagaattt attgatattc cagttttgtt tgatgatgaa
      901 gggaatttta gaataaaaca agttatagat tataaaaaga taataggtaa atcttatgtg
      961 aatggtaaat atattgaaac gaaattaggt aaggttcatt attctaaaac tggttttcat
     1021 gtagttccgt atattaaaaa ggagtgatta aatatgaatt tacagtcata cataggcaaa
     1081 ttagtcaaat taacgcttac taataataaa atattaattg gaaaagtgat agactttgat
     1141 gataaagttg ataattttga tggttataat tcgatagaaa ttgatacagg tagaattaca
     1201 tatgatatat cagaaaataa aattaaaact attgttttac aataatagca tcctttctac
     1261 acagataaag agaaagcggt gctatttttt gcgcttttat aacaactaag gatacaagac
     1321 acataacttc aacaatatcc tcatctaaac ctatcgattt cctaagctca attttaaaca
     1381 caaagtttat ttgaaatgat gcacacctat aaaaatctta atcatcgaaa agtcacaaac
     1441 atctccatat taaaaactta aatagtgcat tccatttaaa ctaaacatcg atattcctac
     1501 caatatagac ttagtaattc gattgttaat acattacttt cataaaataa ctacaaccat
     1561 ca
//
LOCUS       DANJFZ010000227         1548 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.236, whole genome shotgun sequence.
ACCESSION   DANJFZ010000227 DANJFZ010000000
VERSION     DANJFZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.236"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(238..669)
                     /locus_tag="PWN45_002831"
     CDS             complement(238..669)
                     /locus_tag="PWN45_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0624519.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
     gene            1171..>1548
                     /locus_tag="PWN45_002832"
     CDS             1171..>1548
                     /locus_tag="PWN45_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL0624520.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPG
                     FAQSISYYIDQLQMKEILGIIGGN"
BASE COUNT          563 a          219 c          161 g          605 t
ORIGIN      
        1 attcaacttt gcaacagaac ctatattttg ctataattat taaattaatt gcctttgtgt
       61 tagtattccc tggattacta acactttggt tagctgttct aagtgataca ggtgcagcaa
      121 tattagttat attaaattca ttacgtttat taaaaaaata aaaaagccta aatactctct
      181 cgatttgtga gaaagtattt aggctttttg aattaataaa tttcttactt cttatgttta
      241 tttaacaatt agtactggga cagaacttct tttagcaatc ttatgtgtaa catctcctaa
      301 aacatgttta atattcaaat cgactctatg attactcata attactaaat caaattcacc
      361 attatttata tctccagaag taattttttc taagattgta tctttaatat gcccaaaatc
      421 tacatctatt ttatattgaa tatctctttt ttctaattca cgtaaaaatg gagtgagttt
      481 tttctctttt tctctaataa tatcttcttt atgcttatta taatacttga cactaattgc
      541 aagatcattt tctgttacaa cgtgataaat tacaacaact gaatcttcat cggtgacttt
      601 ttctaaatag tcagggatag cactaaaatc attttcgaaa tcatagggta ataatatatt
      661 tttgaacatc tacaccagcc tcctattact tattatttag atgtctatta cttatatata
      721 ccctaaatat gtatgcttat tctcatattc aaattattca aatatattcc ttatcatgta
      781 ttagattcta ttttttatat tatttttgta attctttgta taaacctatt tatttataaa
      841 gcgctctaat acatacctaa atatcttcaa cttataaact actttttatt tcaatgcctt
      901 tttccaaact tcttttatga gatattgtgg ttattacctg acatttacaa tttacacatt
      961 tcgctattat tacgcaaatt attttgattt tataattatg atgactataa tctattctaa
     1021 ttaatctctc cttttaggca taaaataatc tacttataat taaagcatcc actatttcaa
     1081 aattatttgt aaattgagat ttataagtga tttttataca gttatttaaa atctatttca
     1141 taatcaaaaa taataaaggg ggtaataaaa atgaaaaaag aaaaacgttt aaatcttatc
     1201 ttaactgtta tacaacagaa tcagtttaat aaaaaacaac aaatagtgga ttacatggca
     1261 agacattttg gagtttacta tagcttgaca actatttctc gtgacttaca agaattagaa
     1321 atttacaaaa tccctgttga aaataaaaag tatatttaca agaaaataaa tcaaacaaat
     1381 caattaagtg caaaaaaaca attagaaata tttagtgatg agattattga atttataacg
     1441 ctaaataact atgtcttaat aaaaacatct cctggctttg ctcaaagtat aagttattac
     1501 atagatcaat tacaaatgaa agaaatatta ggaattattg gaggtaac
//
LOCUS       DANJFZ010000228         1537 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.237, whole genome shotgun sequence.
ACCESSION   DANJFZ010000228 DANJFZ010000000
VERSION     DANJFZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.237"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            81..401
                     /locus_tag="PWN45_002833"
     CDS             81..401
                     /locus_tag="PWN45_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0624521.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            398..1039
                     /locus_tag="PWN45_002834"
     CDS             398..1039
                     /locus_tag="PWN45_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624522.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(1127..1417)
                     /locus_tag="PWN45_002835"
     CDS             complement(1127..1417)
                     /locus_tag="PWN45_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624523.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          625 a          187 c          250 g          475 t
ORIGIN      
        1 gtcgttcaaa tattattgca gatttgcagc ctatactatc atggccgacg ttttaatgaa
       61 gttatcaagg agttttaact atgaaattta tcattgcaat attattagga ttaatattat
      121 ccattactat tgcttttaca atcatacatc atcctatact tgatcgtttt aatcctttct
      181 taaaaacgga gtatagttat gccaaagtgc caaaaggtac gcaacaatat gttaatatta
      241 cggcttatag tgaaagaggg gaaaagcttg attataaatt aacatttaat ggattttcac
      301 ctagtagaac gtatgtcgaa ataaagcata aagggcaata tgtcatatcg atcacatatg
      361 ttgaaaaaga ggatacacca aaagaggtaa gacaagaatg atagaattaa aagatttaac
      421 catacaaaaa ggtaatacac atattttgaa gaaacttaat ttgaaatttc aatgtggaaa
      481 atcatatgca cttattggta aaagtggatg cggtaaatct acattattaa atactattgc
      541 tggacttgaa aaaacaggaa aacaatatgt ctattttaat ggtcaattag aacaatttaa
      601 atctaatttt tacagagata aattaggata tttatttcaa aattatggat taatcgataa
      661 tttgacagta aatgaaaatt tagatattgg attagcatat aaaaaaataa gtaagaaaga
      721 aaaagaacaa ataaagatac gttatataga acagtttggt ctgtcaaaca gtttaaaaag
      781 aaaagttcat acgctaagtg gaggtgaaca acaacgtgtc gctttaatta gaatgatgtt
      841 aaaagatccg attgttatgt tagctgatga accaacgggt gcgttagatc ctaaaacagg
      901 acagatgatt attcaatcat tatttggttt ggtcgatgaa aataaagtgc tgattttagc
      961 aacacatgat atggctattg caaatcaatg tgacgaaata atagatttag aacagtatag
     1021 aaaagtagca tctatgtgat atccaaaaga taaaattaaa agccaatcaa tgggaaatgt
     1081 tgacttcaac actccgtttg attggctttt atactgtgtt actaagttat gaaagtaaca
     1141 acggcaaact gaaaaaaagc aatataataa tatttaatac gattagtaat aaatgtaatt
     1201 gatttttcgt tttaatatag caaaaaacaa tgactaaaga taacaacaat atcatgattg
     1261 cacctgggaa gaaccataat gctgaagctg tgtaccaaat cattggtaac gccataataa
     1321 taaaagatat aagacaagaa aggataccaa taatattata agttctagtc aaaattacac
     1381 ctccaatttt cactttaaaa tgacatgcgt aaaacaatca ttttaaatac attatacaat
     1441 aaaattaaat tgtgcggtag aagaatttga aattaaattg aattgttttt ggtagttggt
     1501 atatttataa cactctgcaa aagaatgtag caaaatt
//
LOCUS       DANJFZ010000229         1537 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.238, whole genome shotgun sequence.
ACCESSION   DANJFZ010000229 DANJFZ010000000
VERSION     DANJFZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.238"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            61..363
                     /locus_tag="PWN45_002836"
     CDS             61..363
                     /locus_tag="PWN45_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624524.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            619..984
                     /gene="crcB"
                     /locus_tag="PWN45_002837"
     CDS             619..984
                     /gene="crcB"
                     /locus_tag="PWN45_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDL0624525.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            981..1334
                     /locus_tag="PWN45_002838"
     CDS             981..1334
                     /locus_tag="PWN45_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL0624526.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          483 a          240 c          221 g          593 t
ORIGIN      
        1 tattgtttta tgattgttta tgatacgata tgtaaatgat aatcatccag gaggtctagc
       61 atgtctcgtt caaaaaaata cttttactta tctagcttaa tgattatttt aagctttttc
      121 tttaatacaa ataacgtttt cctaagtgga ctttttaatt cttttattaa attaatactt
      181 ttctgcagtg ttattaactc aattgtacta attttgtcta taatttttgc agatcgttca
      241 attaaatcac taaagcctga tgcagattgg attagaattg cgagtaaaag tttgccttgg
      301 attattctaa ttgttatttt agtacatatc ttttcaattg ttcgtacatt cggttttatt
      361 taaaaaagtt aatatgtcat tgtagcctta atacaaaaca atacaatgta tcatgctata
      421 atgagtaaaa caatttgata acgttgttgc gtataaaaaa tattagattt tcgaaatcat
      481 aactatgcat ctaatcgcta tagttataca acaagatata acatataatg aggtttgata
      541 atgcatcgac aatttttgtc gtcgcgttgc caaaacctct tttttaaatt caaactactt
      601 cttttcgagg tgaaccaaat gcaatatgta tatattttta tcggtggtgc tttaggcgct
      661 ttattacgtt acctcatttc ttttctgaat actgacggag gttttccaat cggaacactg
      721 atagccaatt tgactggtgc ctttgtaatg ggattgctaa cagccttaac aattgcattt
      781 ttttcaaacc atccgaccct aaaaaaagct attacgactg gttttcttgg tgctttaacg
      841 actttttcaa catttcaatt agaattaata catatgtttg atcatcaaca atttataact
      901 ttactactat atgctgtaac aagttatgtc tttggtattt tgttatgtta cgtcggtata
      961 aaactaggtg gtgatttatc atgatatcaa tcattttagt catgattggc ggcggttttg
     1021 gcgcaattgc tagaagtgcc attactgatt attttaatca taaatttact tcaaagttac
     1081 ctatcgcaac attgatagta aatctagttg gtagtttttt aattggatta actataggct
     1141 tatcaatttc aatctcatgg ttccctgcgt tctttgttac cggtttttta ggtggcttaa
     1201 caactttctc aacgttagcc aaagaactta cactaatgat gacgccaaaa tttaatatta
     1261 acctttttct caattattca cttttacaat tcatcattgg atttatagct tgttatattg
     1321 gctatcatat ttaaaaataa aatgcttcat tcagcaaata ggtaaattac gacaccttcc
     1381 tgaacgaagc attttttaat tttcatgcaa atttttaagc accatataat gcctaccaaa
     1441 tttcaataat ctttgttgcc gtttaaataa tgtgaatgtc aataaattct ccaaactagt
     1501 cgaaaataaa gggagtggga cataaatccc taaaaaa
//
LOCUS       DANJFZ010000230         1536 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.239, whole genome shotgun sequence.
ACCESSION   DANJFZ010000230 DANJFZ010000000
VERSION     DANJFZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.239"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..230)
                     /locus_tag="PWN45_002839"
     CDS             complement(<1..230)
                     /locus_tag="PWN45_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDL0624527.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSN"
     gene            complement(223..1389)
                     /locus_tag="PWN45_002840"
     CDS             complement(223..1389)
                     /locus_tag="PWN45_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL0624528.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            complement(1403..>1536)
                     /locus_tag="PWN45_002841"
     CDS             complement(1403..>1536)
                     /locus_tag="PWN45_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDL0624529.1"
                     /translation="EKAVRKLRDNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
BASE COUNT          580 a          250 c          199 g          507 t
ORIGIN      
        1 tgattggatt tgtatagatt catattaatc aatcgtatat tgttaagcgt attgcctaat
       61 acaacactcg tgattgttat tattgtataa atcgttaaaa tagaaccgaa cgcttcatta
      121 cctactcgtt gattaataat tggataagct aaaaattgta atcctaaagc tataagcaac
      181 gtaccaatta ttgtctttac gctatccata ataaaaactt tcttagccat gatgacgccg
      241 tccttttgta ataaaataga acaacacaaa ccaaaattca cttacaacta aataatttga
      301 actaaacatt aataatgtga ttggatagat tgctataaac ataacgagta aatctatagt
      361 gtttggatca tagttcctaa tcattttata aactagtagc aaaatgcaaa tcattatgat
      421 aaaaaaccct aataagccaa atgatagaat caactcaata atgatgttat gtggtatatt
      481 tccgattagt ttataatagt taaatggccc atagcctaat aacgaacttt gttgaataaa
      541 gtaaatacct ttttcataaa tcggtcctct tccagaagta ccttctaaat taagtgttcc
      601 accttgtaga tatgaaaatg ttctagtatt cgaacctttt gtaaaaagaa agtaaatcaa
      661 tacactagat atgcttaatg caaaaatata cataatgctt tttactgcga taggtattcc
      721 tcttttaaac gtaataagta taaatgcaaa taagccgtaa agaattaata aaatagcacc
      781 tccacgccct cctggtataa acacaatagg gatatcaatt attgtaaata gaacatatat
      841 ccacttatgt ttaactgaac ctttcataat gaaataaatg cctaatccgg cagtaaatgc
      901 tgaaaggtac gaagcgtttt gatagttcat aagtccaaaa ttgatatagc taggtatctc
      961 acctgtaagt tttggtatta aaattacaaa aataaatgaa acagaaaata tgaaaaatac
     1021 taatttaaaa aatctttcta ccgtagcctt gtttatatat ttaatataaa taccactaat
     1081 tgccgctgga actgcccatg ttaaaaagaa tagaatatta tttttagcta gtttctcttc
     1141 cttatctggt gaaaaataat aaaaagctag ataaagcaaa catatagcaa ttaataatat
     1201 taaccctcgc ggaatttctt gggtgacaat tatcttataa atagcaaaca ccgtagtaat
     1261 taatgctata ccaaccatgg ttgagtaata caccggctct atagggaacc ctaatacttc
     1321 tttagtaaat gtagagatta ctataaatat gttcatgctg ataattgcac aaagtacaaa
     1381 aaatttcata gtctacctct ctttaaaatt tatataattc tactaaccgt tcactctcta
     1441 tttgccaatt caaaatttta gacgccttaa ttgcattttg acgtaaatga ttaaacaaat
     1501 cgtgattatc tcttaatttt ctaaccgcct tttcaa
//
LOCUS       DANJFZ010000231         1519 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.240, whole genome shotgun sequence.
ACCESSION   DANJFZ010000231 DANJFZ010000000
VERSION     DANJFZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.240"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>1519
                     /locus_tag="PWN45_002842"
     CDS             <1..>1519
                     /locus_tag="PWN45_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="HDL0624530.1"
                     /translation="DRIYLNEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFS
                     NLAEKINQEFEINDITKVKVYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDD
                     FVKKIREIQKLILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLH
                     QDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGT
                     TIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVY
                     AELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMI
                     CDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFN
                     KLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYS
                     PRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKL"
BASE COUNT          601 a          191 c          223 g          504 t
ORIGIN      
        1 atgatcgaat atatttaaat gaacaaagtg ctatttattt aaacaacaat aaagatacat
       61 ctttttccat taaaaatagc gaattactcg tattcatcaa aaccactgtg acgaataata
      121 atattacttt ttcaaattta gctgaaaaga taaatcaaga atttgaaatt aatgatatta
      181 ctaaagtgaa agtatatatc cataatcttg tctcaaagga aattatatat tcaacaatca
      241 gaccaccgtt atcatatagc gataatttaa attatatttt aaataaactt tctttacata
      301 atgatgattt tgtgaaaaaa attagagaaa tccagaaatt gatattagct tatgaaaaaa
      361 ctgaaattgg atttggcgag gaattatata aagacattat tcatcatatg aaggcactat
      421 tcaaatgtaa aaactatctt caaattgata ccaaaattga catgattaac aattatttac
      481 atcaagatat agcaacaaat atatctgaag cagcgtactt attgtggctt ttatcaagaa
      541 ataatattgg attcacagat ttgaaagttc ttcacaaccg ttttattgaa aagtatggat
      601 ttgaacaact cgtgaatatt aaagatttga tttcagatat aacaggtttc gggacaacaa
      661 tttttcaaga agaagaaacc gatggaaata atattgtaat gcttaagcaa aaatttttac
      721 atgccttgag aaataacgat gagatagtca taaatgaaaa agatgtagag tcattgatta
      781 acgataatga aatcaatcat tatcatgcgc caatgagtgc agatgtttat gctgaacttt
      841 atttaggacg tttttacaat cagtacaatg agctcattgt tattagtcct ttaacggcgt
      901 cttttaatgc tggcgctaca tttgggcgat ttcatcattt aattgatact gaaactttag
      961 caaaattaga acatgaaaaa ggacattatt atcagaagat gatatgtgat gacaatgtag
     1021 aaatgatttc tataaataac ataccgaaat atccgagaaa tcataatgta ttaactaatc
     1081 atgactcata cgaatattca ttgaatttag gaagtagtaa tagttattca aagtatgagc
     1141 ttaccttaga tgatatttat gttggtgcta cctttaacaa attatattta tattctagcc
     1201 aactaaataa aagggtacta tttgaatcaa acaatatgta taacttttta aaagaatgta
     1261 atttatatcg attgttacga gaaatttcaa tggaatctgt gaaatgcatt gaacctatga
     1321 atgatgtgag tattgattca tttagttatt caccacgaat cagatataaa aatgtcattc
     1381 ttaaaccagc atattggaaa attaacgaga tggtattacc tttacctaaa aatgaagaat
     1441 gggatcaaca atttttgaaa tatcaagaac aattcaatat acctaatatc gtgaatttag
     1501 tttatggaga taacaagct
//
LOCUS       DANJFZ010000232         1500 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.241, whole genome shotgun sequence.
ACCESSION   DANJFZ010000232 DANJFZ010000000
VERSION     DANJFZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.241"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..269)
                     /locus_tag="PWN45_002843"
     CDS             complement(<1..269)
                     /locus_tag="PWN45_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0624531.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVE"
     gene            558..1253
                     /locus_tag="PWN45_002844"
     CDS             558..1253
                     /locus_tag="PWN45_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0624532.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          528 a          217 c          275 g          480 t
ORIGIN      
        1 ttttcaactt gtttaaaatt acgtttaatg ccgttataag aatatacaac attatataat
       61 tttacttggg cgtcatggac acttgagaag ggcattctta catcgtccat atttttaact
      121 tcgtgtcgaa atgctttttc agctttttgt ctttctgcaa gagacatact tagccattct
      181 tctttgctaa ataaatcttc gaatcgaaaa tcaaatgcgg ttcttttact ttctgctctt
      241 aatgctaaaa cacgctcatg atatgtcacg ttttattcct ccctctaatc attcaatcga
      301 atgataaatc acagtattaa ctttattact aatataatgt ttttacaata aagaagtgac
      361 gatttttcaa catttttaaa aataaaaaat ataagcaacg attaattgta tgtttcgaat
      421 ggcttgtaat gtaatttatc tatgaaaatg aagcgagtta ttatgaccgt tatatgaatt
      481 gtgatacgct ttttaaaagt aatatgtatt tatgatttac cctgaaaaaa taaaaagaat
      541 caaaggagtc gatcattatg acagtattaa cagataaagt agcagtagtt acaggtgcag
      601 gtagtggtat tggagaagca attgcaacat tactacatga agaaggggca aaagttgtct
      661 tagcaggtag aaataaagaa aaattacaaa acgtagcgaa tcaattgtca caagatagtg
      721 tgaaggtagt gccaacagat gtaacgaata aagaagaagt cgatgaattg ataaaaattg
      781 cacaacaaac attcggtggt ttggatattg ttatcaatag tgcggggcaa atgttgtcgt
      841 ctaagattac tgattatcaa gtagatgagt gggatagtat gattgatgtg aatatcaaag
      901 gcactttata tacggcacag gctgcattac caactatgtt agaacaatca agtggccatc
      961 ttattaacat tgcatctatt tctggctttg aagtaacgaa aagtagtacg atttatagtg
     1021 cgacgaaagc agcagttcac actattactc aaggattaga aaaagagttg gcaaagacag
     1081 gcgttaaagt aacaagcatt tctccaggaa tggtagatac agccataact gccgcataca
     1141 atccaacaga tcgtaaaaaa cttgaaccac aagatattgc agaagcagta ttatatgcat
     1201 taacacaacc aaagcacgtt aacgtgaatg aaattacagt gcgtcctgta taaaaaggtg
     1261 tttgaggtct ggggtatagg catttaatgt aatagctatc gattttgtta atgttagtaa
     1321 ggcaataatt acatgagggc ataataggca attattcctt gtacaattct cagtaattgc
     1381 tgattttaac aatcaagtta atgaaggcga cgatgttagt gttatggttt ataatctgta
     1441 atgttcataa ttatagatta taaaaatttt ttcattttcc accacatttt aggagttact
//
LOCUS       DANJFZ010000233         1440 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.242, whole genome shotgun sequence.
ACCESSION   DANJFZ010000233 DANJFZ010000000
VERSION     DANJFZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.242"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>1440
                     /locus_tag="PWN45_002845"
     CDS             <1..>1440
                     /locus_tag="PWN45_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0624533.1"
                     /translation="FINSLVWRKDGSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMP
                     QKNHPLNIWALHKLLKQCNLGKRDFLWTQYISINNEKVFEIINWLFSNYKKLDEETAE
                     KYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYAS
                     VLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNIN
                     LEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGA
                     YGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFN
                     NSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDKESKIL
                     VPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLSSKFNFEKREKEIPFEDRD
                     SCYFLAMGYFYNKEDSNEIIKGYENNYDRG"
BASE COUNT          581 a          141 c          226 g          492 t
ORIGIN      
        1 tttataaata gtttagtatg gagaaaagat ggtagcataa gtaaacatac taaatgctat
       61 atatccgata atgttattcc ttatgatgca tttagaaata attttttaga tgtcttatta
      121 ataaaaatgc ctcaaaaaaa ccatccgtta aatatttggg ctttacataa attattgaaa
      181 caatgcaatt taggaaaaag agatttttta tggactcaat atatatctat aaataatgaa
      241 aaggtttttg aaattattaa ttggctattt agtaactata agaaattaga tgaagaaact
      301 gcggaaaaat atatgatttt tttaacgtgg atattttctg caactaataa taaattaaga
      361 gatctcggta ctaaatcttt agtaaaatta ttcaaaacat ttccaactaa aattattggt
      421 ttattaaaat tatttgaaaa taacaatgat ccatatatag tcgaaagatt atatgcttct
      481 gttttagggg cgacacttcg aatcgacatt tgtgaaatac atatagagat tgctaattat
      541 atttatgaag aaatatttga taaagaaatg gtatatcctc atattttaat gagagattat
      601 gctaggcaaa caattgaata tattagttta tcaaaagata tatctaatat aaatttagaa
      661 aaaattaggc caccttataa aagtaattgg tataaaaaag aatattcgaa tttaaatata
      721 gacgattaca taaaatcttt aaaaaacaaa ttagatagcc atttacattt ttctatcgat
      781 aaaattaaaa actcaatgac aacggaatat ggaagaggaa ctggggcata tggcgatttt
      841 ggcagatatg tatttggtta tgctgtacga aactgggtaa agggttttaa aagtgatcaa
      901 gacttaagta atatagcatt aatgaggatt tttgaaatgg gttatgatgc aaaattacat
      961 ggtgaatttg atatgtgggt taatcgttat gataacttta ataattcaat tgaaagaatt
     1021 agcaaaaaat atcaatggat tgcttactat gaaattttag ctaaacttgt agataaattt
     1081 cctgatgttc aatattctgg actatgggat gattatatta gagatattga tcctacttta
     1141 ctattacttg aaattgataa agaatcaaaa atactagtac cctcaccact tccttcgcat
     1201 caatcgaatg aatgggtaaa aaataccaaa gtttttgatg aaacaaaatt atttttagaa
     1261 atagatatag acaatcatag atatatatgt ttaagctcaa agtttaattt tgaaaagaga
     1321 gagaaagaaa taccatttga agatagagac tcatgttatt tcttagcgat gggatatttt
     1381 tataataaag aagatagtaa tgaaattatc aaaggttatg aaaataatta tgatagaggt
//
LOCUS       DANJFZ010000234         1439 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.243, whole genome shotgun sequence.
ACCESSION   DANJFZ010000234 DANJFZ010000000
VERSION     DANJFZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.243"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(1..570)
                     /locus_tag="PWN45_002846"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="PWN45_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(617..1258)
                     /locus_tag="PWN45_002847"
     CDS             complement(617..1258)
                     /locus_tag="PWN45_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDL0624534.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          558 a          209 c          153 g          519 t
ORIGIN      
        1 taaaaagaaa aacatcgtga taataaccca agttaaaaat attattatat tttgtgactc
       61 tgtcgtcaaa agaatggtta aagtaaatat tgaagcgctt cctaaaaaaa agtacgcaaa
      121 aactattaac aatccttgtt tgaaagtcgg tattaataca actctattct tattaaccac
      181 gttggtatta aaaattttta atgttaattt tttatttaga taaaaataaa aaattaacat
      241 ccctattaac atgataaaaa atacagtgat tgctagttgt ttttcaaact gaacattaaa
      301 gaagttaccg tactttctca atataattcc aaatagaact gaacttcctg caaccggcca
      361 aaaaacatta tttttaacag gctcgactat atttaacctt taaaattcat tttcgctaat
      421 ttttgcgtat tttttgggca aaaaccaatt aatcataggg aaaaaataag taatccaagt
      481 acttactaaa tcgaccatga aatattcatt attatatcta ataactcgat atttaggatt
      541 tttataaatt tgtctagatt cacaaagcac tgtttaccac ccctaattaa caattgaaaa
      601 ctaactaaat ttgtaactat ttattagact ttaagaatat atgtactttt ttatctataa
      661 ttgaatgcat attcataaac ataaatagca tagtcatgaa aagccaagaa acatataaga
      721 ttaaattttg cacattttca aataccaaca agtaaaatgc cccataagac atgaaaccag
      781 taaataggta ataaaaaatt gtgaaacaaa tatttttaaa ggagggtaat aatcttaatt
      841 taagttcatt atttttactt tcattataaa tatttaatgt taatttctta tttagataac
      901 aataaaaaaa tataatctct aaaaacccaa tgaaacagct gctccaaaca attagttctt
      961 ctaattgaaa atcaaataaa taagtatttc ttcttacagt accaccaagt aacacaatac
     1021 ccgcgaatat ggtccatcct atcgatttat ttttaactgg tttgacaata tttaattttt
     1081 cataatcatt cttagtgatt ttgacatatg ttttcggtat gagccagtta ataaatggaa
     1141 agaagaagac aatccaatta cttgccaaat caatcattaa atattcacta tcgtatttga
     1201 ttattcgata tttagggttt ttattaataa ctttagattc gcaaagcaat gtctccacat
     1261 ccctttaatt ttatgtgtaa tacatttttc gatacttcaa aagacattca aatactatca
     1321 agttactgtc atcaaaggtt ttattaactg atattttcat atttttaatc tgaatttagt
     1381 actttcactt aatttttaag tatgacatga atgtttttat ctataattaa agctatatt
//
LOCUS       DANJFZ010000235         1436 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.244, whole genome shotgun sequence.
ACCESSION   DANJFZ010000235 DANJFZ010000000
VERSION     DANJFZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.244"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(140..358)
                     /locus_tag="PWN45_002848"
     CDS             complement(140..358)
                     /locus_tag="PWN45_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624535.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(420..704)
                     /locus_tag="PWN45_002849"
     CDS             complement(420..704)
                     /locus_tag="PWN45_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624536.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(792..1361)
                     /locus_tag="PWN45_002850"
     CDS             complement(792..1361)
                     /locus_tag="PWN45_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624537.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
BASE COUNT          472 a          205 c          205 g          554 t
ORIGIN      
        1 atcaactttt tgttaatttt ttattaagac tgattaatta ttgagaatat ttattgtttt
       61 taaaatctca taataattca gtaatcttgt ttttatttaa aaggcgaaac attaaaataa
      121 ttaaataaaa atattgcgtt taatttacag cgtcaaatat acttatttct aatgctttgg
      181 ggtctactga aacaagtaaa gaatgatcga tgttactaat attgccattc tccaaattta
      241 tttctgtgag tatttggaag ctaccattag gcaacggttt aacaatagac aattgctttt
      301 ccgcttgttg tattaaaaaa ggttttgtag attgattatt aatatgccat tcactcatgt
      361 atgtttttca ctcctgcttt aaaatagggt tagaaagttt atagttgaga cattcatgtt
      421 caaccaaaat tttgttcgaa ttcaataaat gtcttgttta aaatagaaat attgtaaatg
      481 ttatcgtcca aaacttcacc agttaagtat ttgttttgaa ttaaaatttg gcagttagtt
      541 aagaagtctt gataatcacg atcgcaaaaa tagttttcac gtgcatcttt agcatcgcca
      601 aaaaagttag cgactgtttc tgtttctcct ttattcgaac gttcaatata taatttgtaa
      661 aatttagcta ttgtatactt ttgttcttta gttagttcat tcaaaatatt gggcctcctg
      721 aaatatcatt tgtaatctat acccaattta ttgcaaaaca caaactaatt taactatttg
      781 atgaaactgt gttaataagc tttaacaagc cttagtttgt atggatctat aaaattatct
      841 ttaattgcat agggtgaaat aatatgtagt ccataacttt taactgattt ttcacttaca
      901 ccaaatttat aagcttggta gataatttta gtacaatacg taaatttttt gctgttcaaa
      961 tttaatgtaa ctagataacg atgatttgta ttctcatagt ttttcttaac ccattcagcc
     1021 gcttttttac ctgcaccagg atagctgcaa cgataaactt tcatccaatc atttttgcca
     1081 cttgcataat tatatttaaa agattcgaag gattgtgtag ttggtttgtc gccaggcccc
     1141 tcaatttgta aaatcgtttt atcatcaatc gcgatactac aatgaccaaa aaatccccac
     1201 atgacagggc cttttgtaac aataatatca ccaggttgta attggaattt gtcatcttga
     1261 atttctgaat acttattatc tgcaattgtt tttggtgagt ttattgggga tacgacaacg
     1321 aataatataa gtaaaattat cgttcgttta atatagttca cttaaaagct ccttgttgaa
     1381 gaaatatatg taaatagtct taaattagaa ttgtaatctt taataagctt gtaaga
//
LOCUS       DANJFZ010000236         1374 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.245, whole genome shotgun sequence.
ACCESSION   DANJFZ010000236 DANJFZ010000000
VERSION     DANJFZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.245"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(1..403)
                     /locus_tag="PWN45_002851"
                     /pseudo
     CDS             complement(1..403)
                     /locus_tag="PWN45_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(524..1243)
                     /gene="mecA"
                     /locus_tag="PWN45_002852"
     CDS             complement(524..1243)
                     /gene="mecA"
                     /locus_tag="PWN45_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL0624538.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT          423 a          258 c          162 g          531 t
ORIGIN      
        1 tcatttaata accaacttac tttatatccc attttcttta aaccttccgt tcgttgaaga
       61 atctgtttat aaggaattgg cgaatactgt agctcaataa catatttatt attaatcaaa
      121 atatccggaa tttgttttat ttcttttaaa atggctctat ctctacttta catccttgtt
      181 gctgtatcat ctgtgctaaa atcaatttac ttttataatg tttaatcgtt tcattattaa
      241 aacatttttg ttccgctaaa tgtttatgtg caaaatgact aattactttg agcccacgct
      301 ttaaaataac ttgcttgcca cacaccggac aaaaatattg tgtctttctc aatgcagtag
      361 ttgctaatac gcgttccttt tcttcattta aagctactaa catatcttca cctcaaaata
      421 ttacacgtaa gtttaactac tattactttt tcagcttttg aaatcaataa aaaaagagac
      481 tccaaagagt ctcctttcat agtgcgttgc ttaattaata tcgttattca gttgtctctg
      541 gaaaataacg tcgaacttga gctgttacgt tatgactcat aattatttta gcatagtcat
      601 ttaaataaac ttctgttctg tctgttggat aagcaaattc aagcaattga ctgtaactat
      661 cattaatgac ttcttgatca acatgactat caaaatatac agcataataa taagtaccat
      721 caaccatata taacaaatct tcaaactctg tagttattgg attgctatga tatgcataat
      781 taataacatc ttctaaatcg ttaaatttca caatgattgt tcttgtattt ttacgtgctg
      841 aagacttttg acgtttagaa ccttgagctt ctttttcttt tgttcgttgc tcgaataatt
      901 cttctaattg atcttcacct tctaatgttt gagctaacaa ttcttgaact tgttcatcaa
      961 attgatcagt tgcatcatca tcagacatat tcatcatatc ttcattttta gatttagaaa
     1021 ttgtgacttc gacacctttt tcaaaggcat gtacttgaat ccataatgga ccttctacaa
     1081 caaaatcttc ttcttcgtta atttcatcca tcattgacca aaagaattct tcgccacgtt
     1141 tgcgatttgt ccataaatct tcacgactaa atccacgggc ctcgatatcg ctatatgtta
     1201 taaacaattt tacagttgta tcatctactc gttctattct catatcatct cactccttac
     1261 agtcgatgaa tagtatccac attgtattaa agcatcaaac aaaatacaat ttatttgttt
     1321 gatttctttc tctaaaatat tttggtaact tataataaca tattcgttgt agca
//
LOCUS       DANJFZ010000237         1373 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.246, whole genome shotgun sequence.
ACCESSION   DANJFZ010000237 DANJFZ010000000
VERSION     DANJFZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.246"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..580
                     /gene="icaB"
                     /locus_tag="PWN45_002853"
     CDS             <1..580
                     /gene="icaB"
                     /locus_tag="PWN45_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDL0624539.1"
                     /translation="LKEFLYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPA
                     TGFIITGHVGEENFHNLDMISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKAS
                     EATIIKDLNKSEKYLTKNFKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVT
                     PNSNDYYIPRILISDDAFEHLIKRWDGFHEKD"
     gene            567..>1373
                     /gene="icaC"
                     /locus_tag="PWN45_002854"
     CDS             567..>1373
                     /gene="icaC"
                     /locus_tag="PWN45_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDL0624540.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTM"
BASE COUNT          493 a          191 c          201 g          488 t
ORIGIN      
        1 cttgaaagaa tttttatatt acaagaaaaa aggtaagttt ccaaaacgaa gtgtatggat
       61 taactttgat gatatggatg aaactattta tgaaaatgct tatccaatct taaaaaaata
      121 taaaataccg gcaactgggt ttattatcac aggtcatgtt ggggaagaaa actttcacaa
      181 cctcgatatg attagtaaaa aagaactaaa agaaatgtat aaaactgggt tatgggaatt
      241 tgaaacacat acccacgatt tgcataactt atctaaaaat aataagtcaa aattaatgaa
      301 agcttctgaa gctacaatca taaaagattt aaacaaaagt gaaaaatatc taactaaaaa
      361 ctttaaaaag tcgcagaaaa ctatagccta tccttatggc ttgatgaatg acgataaatt
      421 accggtaatc aaaaaagctg ggttaaaata cggtttttca ttagaggaaa aagcagtcac
      481 tccgaactcc aatgattatt acatccctag aatattaatt agtgatgatg cttttgagca
      541 tttaattaag agatgggacg gattccatga aaaagattag acttgaactc gtatatttac
      601 gtgctattat atgtgcaatt attattatca cacatttact tacacaaatt actttaaaac
      661 atgaaaatat ggagggtgga tccttagtgt tacaatttta cattcgtaat attgtgattt
      721 ttggtacacc ttgctttatt atcttgtcac agttactgac aaccttgaat taccaaaaag
      781 tcacctatag atacttaact acacgcgtaa aatatatact tattccttac atattaatgg
      841 gattgtttta cagttatagt gaatcattat taacagattc aagtttcaat aaacaattca
      901 ttgaaaatgt cctattaggt caatggtatg gctattttat cgttgttatc atgcaattct
      961 ttattttgag ttatatcatt tttaaaatta actataacct attcaacagt aaaatattat
     1021 tattgttatc ttttatttta cagcaatcat ttttatatta ctttacgaac aacacagcgt
     1081 ttcacgatac cgtgctacac tattatccat taagtgaaaa tactataata ttcggatgga
     1141 ttttttattt cttcttaggt gcatatatgg gttataacta cgaacgtgta ttaaatttct
     1201 tagaacgtta tttagttatt atgattgtat tagctgtagc tacttatttt gtgtttattg
     1261 cgttagcaaa tggagactat tggaacgtta ccagcttttc atattcatta acaccatata
     1321 atagtattat gtttattgtt atcttgggta tttgcacgca ttttaaaaca atg
//
LOCUS       DANJFZ010000238         1355 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.247, whole genome shotgun sequence.
ACCESSION   DANJFZ010000238 DANJFZ010000000
VERSION     DANJFZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.247"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..1318
                     /locus_tag="PWN45_002855"
     rRNA            <1..1318
                     /locus_tag="PWN45_002855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DANJFZ010000239         1321 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.248, whole genome shotgun sequence.
ACCESSION   DANJFZ010000239 DANJFZ010000000
VERSION     DANJFZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.248"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(210..1217)
                     /locus_tag="PWN45_002856"
     CDS             complement(210..1217)
                     /locus_tag="PWN45_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL0624541.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1233..>1321)
                     /locus_tag="PWN45_002857"
     CDS             complement(1233..>1321)
                     /locus_tag="PWN45_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDL0624542.1"
                     /translation="DIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          449 a          249 c          205 g          418 t
ORIGIN      
        1 aatcattatt ttgctcttac atatatttta atgacctaac tgattatgtt ccatggaata
       61 catttataat atagcctcct aattaaaatg ctttgtcttg gtcattctac gtaaattcta
      121 taaaatatgt tatctactta cataaaaaac tgaacttcaa taccaccata tgtttgtgat
      181 actgaagttc agtttagttt tattttcaat tagaaaaata agttaagtat atagaatagt
      241 aaacctgcta acaatgctga aataggtaat gtaatcaccc atgtaatgat cattcgttgc
      301 gcagtgctcc attttacacc tttagctcgg ttagaagcac caacacctaa gattgatgat
      361 gacacaacgt gagttgttga taatgggaaa tgtagcgatg atgcaacaaa aattgttaat
      421 gcagatgata aatcggccgc agcaccattt gctggacgta ttttcataat attaccacct
      481 acagttttga taattttcca gccaccaatt gcagtaccaa gccccattgc tgtcgcacag
      541 gcaaatttta cccataactg tggttcaaca ctgccatcat tctgtacatt agcgacaatc
      601 aatgccaacg taataatacc cattgatttt tgcgcatcat tcgtaccgtg agagaatgat
      661 tgtaacgctg ctgtgaaaat ttggaaaaat ctaaagttac gattcgctct tgttaaattt
      721 gcatttttaa agataacttt aaaaattgaa tacatcaaga aaccaacaca aaatgcgata
      781 atcggtgaaa cgattaatac aataataatt tttgtgaaac cttggtaatg taacactcca
      841 aatgagcctt cagatgcgat tgctgcaccc gcaattgaac ctataagtgc atgtgaagac
      901 gaacttggaa ttccgtaaaa ccaagtagct aaattccaaa taatagccgc aagtattgca
      961 gctaacacaa caactaatcc attttccaat ttaaatggat cgacaatgtc tttagtaatg
     1021 gtgcctgcaa cgcccgtaaa tgttaaagca cctataaagt tcatcactgc tgccattaaa
     1081 attgccgttt taggcgttaa cgctctagta gatacagcag tagctactgc attggctgta
     1141 tcatggaatc cattgataaa gtcaaatatc agcgagaaaa taactacagc tatagtgacg
     1201 atgattatat atgacataaa tatatactcc ccttagctat ttttcataat aatagtttca
     1261 aaattatttg ctacgatttg acatttatca gcgatttctt ccatgctttc ataaatatct
     1321 t
//
LOCUS       DANJFZ010000240         1256 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.249, whole genome shotgun sequence.
ACCESSION   DANJFZ010000240 DANJFZ010000000
VERSION     DANJFZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.249"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(380..520)
                     /locus_tag="PWN45_002858"
     CDS             complement(380..520)
                     /locus_tag="PWN45_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDL0624543.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
     gene            complement(517..1086)
                     /gene="agrB"
                     /locus_tag="PWN45_002859"
     CDS             complement(517..1086)
                     /gene="agrB"
                     /locus_tag="PWN45_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDL0624544.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
BASE COUNT          509 a          164 c          157 g          426 t
ORIGIN      
        1 caaaaaagct agggaatatt acaaatatac tatcagaaat tttggtgata tatgcatata
       61 taactatata catgtagtta gcacagtata gaataatctg cgaagtcata atcaacaaaa
      121 tagaatacca tttgattttg acaaaataca ttataataat aaatgaagtt aatatgatta
      181 aggacgcgct atcaaacatt ttaaatagaa ataacgataa tgtcgaaatt actatgatga
      241 aaaaataatc aagtttactg tacttaatac cactaattat agctggtatt gtaaacatta
      301 atatcatttg agttaatacg aataaaacaa aattataact atttaataat tccacctact
      361 atcacactct ctatttaaat tattcgtgta attgtgttaa ttcttttggt acttcaactt
      421 catccattat gaagtcacaa gtactataag ctgcgatgtt accaatgttt tttaaaatcc
      481 cagtaataaa atcaaaaaat aagttaaata atgtattcat tttaagtcct ccttaataaa
      541 gaaaataggt aataatgtaa tagcttctat tatgatgcct aattgaatga attgggcaaa
      601 tggctctttg atgataagtg tgataatgaa aagggttaaa ctaacaataa tcgcataata
      661 tttttttcgt ttaataagtc gcacaggaat gggcttcttt ttagttgctg caggagcata
      721 tactgagatt acacctaaag aaataactgt taaaataatc ataattaaaa agttaatatg
      781 aaaatttact attactaaag gtaaaagtat aaatagtata atactttcta cataacacca
      841 aaaagaagaa ggtgcatgtg caccatgtgc atgtcttctt attaaataaa atgttaaatt
      901 cgtaattaac gtaaacagaa aaatgtttaa aatataggca atagtataca taacaattaa
      961 tttacctata tttttagcta agacctgcat ccctaatcgt acttgcaaaa attgaatatg
     1021 atctaagtta tttctctttt gaagatacgt ggcaaactgg tcaattttat tatcaaaata
     1081 attcaatttt acaccactct cctcactgtc attatacgat ttagtacaat cttttatcat
     1141 tatattgcct aactgtagga aataaatact taactgttaa atgtaatttg tatttaatat
     1201 tttaacataa aaaaaattac agttaagaat aaaaaacgac tagttaagaa aaattg
//
LOCUS       DANJFZ010000241         1178 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.250, whole genome shotgun sequence.
ACCESSION   DANJFZ010000241 DANJFZ010000000
VERSION     DANJFZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.250"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(401..898)
                     /locus_tag="PWN45_002860"
     CDS             complement(401..898)
                     /locus_tag="PWN45_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0624545.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
BASE COUNT          420 a          176 c          135 g          447 t
ORIGIN      
        1 aaaatagata cactaatttt aaaacaaggt aaaaaaatag aattattaaa acaacgcaaa
       61 caaggcttac tacaaaaaat gtttgtgtaa ttctgataac gtcctattat gttaattgaa
      121 aatagacaaa aataaattga gagcatccct taccgcaaag tgaaggatgc tctatttttg
      181 ttgaaattaa tataagcttc attctgatag atagtcatta ataattgttt gcaaatcgtt
      241 attaagtacc gttgttacag cgtcatcatt cataaaatcc tcgtagattt tatcaagaat
      301 ttattcctct tacagacatt cgcgagaagt ccgttttaat tttattagaa gtaattcagg
      361 tttgaaccta cctaaatgaa tatatgagtt atcttttata ctacaaaata tattcagatt
      421 tcaataatga cataaaatag gcatctttat atttaccttt agtgtagaat tgctctttaa
      481 gtaatccttc tgttttaaat ccttgtgact cgtatatatg aatagctttt ttgttatctg
      541 catcaacata tagatagatc ttgtgcatat ttaaaatatt gaatgcataa attatcgctt
      601 tttcgaatgc gaattttgca taacctttac cactgaactc aggtttaata attatttgta
      661 tttcacaatt acgatggatg taattaattt ctactaattc aacaatacct acgacttgat
      721 tttcatcttc aacaataaaa cgtctttccg attcatctaa taaatgctta tcaaataaat
      781 gttgaagttc cgttaaggat tcataaggct cttcaaacca ataagacata atagaatatt
      841 cattatttaa ttcatgaaca aaaagtaaat cactatactc taatgctctt agtttcataa
      901 tcccactccc aaaattttct tatatatttg cattataaat ataaataacg aataagtcat
      961 tattcactat gaataaatct attttaacaa ttcactacat actaattctc attttcttat
     1021 tattctctaa tatctctgat ttattactca gtgaaagatg ccctattttt atcagtatat
     1081 agtttttatt ttgatagata ttgattaata atcgtctgta aatcattgtt aagtaaggtt
     1141 gtgatagcgt cgttattcat aaaatcttca taaatttt
//
LOCUS       DANJFZ010000242         1168 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.251, whole genome shotgun sequence.
ACCESSION   DANJFZ010000242 DANJFZ010000000
VERSION     DANJFZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.251"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..668
                     /gene="sek"
                     /locus_tag="PWN45_002861"
     CDS             <1..668
                     /gene="sek"
                     /locus_tag="PWN45_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HDL0624546.1"
                     /translation="SAQGDIGIDNLRNFYTKKDFVDLKDVKDNDTPIANQLQFSNVSY
                     DLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRNIPINIW
                     INGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFY
                     SGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLDVDISYK
                     ETI"
     gene            756..>1168
                     /locus_tag="PWN45_002862"
     CDS             756..>1168
                     /locus_tag="PWN45_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0624547.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQV"
BASE COUNT          476 a          153 c          187 g          352 t
ORIGIN      
        1 ccagcgctca aggtgatata ggaattgata atctcaggaa tttttataca aaaaaagact
       61 ttgtagattt aaaagatgta aaagacaatg atactcctat agctaatcaa ctacaatttt
      121 caaatgtatc ttatgattta atttcagaat caaaagattt taataaattt agtaatttca
      181 agggaaaaaa acttgatgtt tttggtatta gttataatgg ccagtgtaac aataaataca
      241 tatatggtgg agtcacagct actaacgaat atctagataa acctagaaat atccctataa
      301 atatatggat caatggaaat cacaaaacta tttctaccaa taaagtttcg acaaataaaa
      361 aatttgttac cgctcaagag attgatgtca aattaagaaa gtaccttcaa gaagaataca
      421 acatttatgg acataacggc actaaaaaag gagaagaata tggtcataaa tcaaaatttt
      481 attctgggtt taatattggt aaagtaacgt tccatttaaa taataatgac actttttcat
      541 atgatttatt ctacacagga gatgatgggt taccaaaaag ttttttaaaa atttacgaag
      601 acaataaaac tgtagagtct gagaaattcc atttggatgt cgatatttct tataaagaaa
      661 cgatataaat ctattaatta tgtcataatc attattttcc gggtagcatg cctaccctta
      721 ttattttttg ccaattttga ggagggagaa gcaaaatgtg gttagaaaaa tttaaaaata
      781 agaataaaga aactaaatat agatattacg agaagtacaa agaccctctc actgccaaat
      841 ggagacgtgt tagcgtggta cttaataaga atggtaagca atcacaaaaa gaagctcaaa
      901 gactcttaaa tgagcgtata gaagaaaaac tgaatgataa gaaacctact acacttaagt
      961 cactaacttt ccatgtcgca tgtgatgagt ggtttcagaa ttatatcaaa acgtctgggt
     1021 caaaaagaac aactattaaa actaaattga gtaagctaaa cactttgaag aaatttgtag
     1081 atgaagatat tttaatcaat aaaataacac tttcatatgc gcaacaagtt ttcgatgaaa
     1141 tggatagtaa aggttatgta tatcaagt
//
LOCUS       DANJFZ010000243         1166 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.252, whole genome shotgun sequence.
ACCESSION   DANJFZ010000243 DANJFZ010000000
VERSION     DANJFZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.252"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..933
                     /locus_tag="PWN45_002863"
     CDS             <1..933
                     /locus_tag="PWN45_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0624548.1"
                     /translation="VICPLIFNYTLTLNLTCYILVVISIFLSLIYSIMTYTFPWSMFV
                     TFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFISTIQSPIVKGLS
                     MWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLDR
                     ALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYTN
                     FLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVLI
                     KDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          366 a          200 c          163 g          437 t
ORIGIN      
        1 gttatttgtc cgttaatttt caactatact ctgacgttaa atttaacatg ctacatctta
       61 gttgtcatat cgatattttt gtcactcatc tatagcatca tgacctatac tttcccttgg
      121 tctatgtttg taacatttat aataatcagt attatcatgc tattcaaaca tagatattta
      181 ttttctcata cttctagtcg cttatttatt aaaaaattat tatctttttc atataaattt
      241 gtactctaca catcagcact tctatttata agtacgattc agtcacctat tgtgaaaggt
      301 ttgtctatgt ggttagctat attatgtctt tccctgttac taacctttat ttgttatctc
      361 gtttggacag tatcatatag acatagatca tacaacaaag aagttgatat cataattgtg
      421 ttaggtgctg gtatttttac cgaatttgtc acaccaatgc ttgcagcgcg tttagatcgt
      481 gcacttgata tctatcaaca acaagcatca gcaacaaaga tcattgtatc tggcggacaa
      541 ggaccagacg aaccaatttc tgaagcttta gctatgcaac gttatttgat tgcacatggt
      601 gttgataaaa cagatatact aatggaatct cactctataa atacatacac aaacttttta
      661 tattctaaac aaatcatcaa caatttgtat catgaagcag ttaaaattgt ttgtgtaaca
      721 agtcagtttc atgtattgcg cgccttacga ctagcccaaa aattaggttt atctatcgat
      781 ggtgtcggaa gtcatacacc atatcacttt ttcttacatg tgctcatcaa agattatcta
      841 ggcgtaatgt atcagtatag attgttatta acgttatatt gttcaagtag ttggtttata
      901 tgtctttacg ccgcttttat ttataatagt taatccacaa acaaactatt attataggtt
      961 gtttctaata ttgcgtcaaa ctatctttaa atattcgtta atccttgatt agacaattgt
     1021 tatattgaaa ataaataaac taaagagtct gggacattaa tccctaaaaa aaacagcagt
     1081 aagataattt tcaattagaa aatatcttac tgctgttctc tatttataca atacttcgta
     1141 ttgaatggct tcgctatgcc catctg
//
LOCUS       DANJFZ010000244         1141 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.253, whole genome shotgun sequence.
ACCESSION   DANJFZ010000244 DANJFZ010000000
VERSION     DANJFZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.253"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..1061)
                     /locus_tag="PWN45_002864"
     CDS             complement(<1..1061)
                     /locus_tag="PWN45_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624549.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LYTIGTIQYLILFISLLIF"
     gene            complement(1054..>1141)
                     /locus_tag="PWN45_002865"
     CDS             complement(1054..>1141)
                     /locus_tag="PWN45_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL0624550.1"
                     /translation="DNKIELLREGDIKFEEIKERLGTGIIYE"
BASE COUNT          452 a          162 c          110 g          417 t
ORIGIN      
        1 ataaaaatta ataacgaaat aaataatatt aagtattgaa tagttcctat tgtatataag
       61 taaccaccca taagtaaaaa tgcgatatta aaaataaaat ataacgtttc tgcataatta
      121 agcatcactc ttaagttact actgctagaa agtttattta tagtttgtgg acgagttaac
      181 cctgaagaaa tacctaaaaa tatcatagaa atagtaactg ctatgaaact ttgatatgta
      241 tatattatta aaaatattac taaacaaatt tctgacacaa acttataatt caagtattta
      301 gttaaataac gtgaagatac aaaacccata actgtgtaac aagttaatac tgaaataaat
      361 tgtaaattat tcagttttaa atcattatat atattaattg gaataaatcc tataaaaaat
      421 cctaatatta acgctcttaa aaaagaataa tgcaatatcc aaaacttctc ttctggtaga
      481 aatttcttta tctttccctt tgtttctcct attaaattta attccctatt ttcaactaat
      541 gtgcatcgaa taattataat tgttagaatt gaaaatatac cagtcattat tataggccat
      601 ttaatattta ttccataaag ataactacct ataatccctg aaattaataa tgataggaac
      661 atatagctat ttgacttatt ttgtacgtat ttctcatttg ttatatttct tttaattatt
      721 gcggtatcaa cacccgccat cattgagtaa cttaacccta ataatatttg tgccactact
      781 aaaattaaat attgattttg atataataac aacaataaac cgatgatttt gaatatttca
      841 ctaacaacta atttattaga atcttttaag ttacaaattt taaaacactt ctctttgtat
      901 agagagaata aaaatgctgc aatgccataa gacgccatta atatttctaa ttgtattatg
      961 gaataacctt gaatcaaaaa gtaaattaat aaaaacggta aataaaagta tgctcgtgaa
     1021 aaaattcggt ataaataaat cccaagtatt aacttattca taaataatac ctgtacctag
     1081 tctttctttt atttcttcaa actttatatc tccttctctt aataattcaa ttttattatc
     1141 t
//
LOCUS       DANJFZ010000245         1122 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.254, whole genome shotgun sequence.
ACCESSION   DANJFZ010000245 DANJFZ010000000
VERSION     DANJFZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.254"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..662)
                     /gene="sarU"
                     /locus_tag="PWN45_002866"
     CDS             complement(<1..662)
                     /gene="sarU"
                     /locus_tag="PWN45_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDL0624551.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVL
                     IK"
     gene            989..>1122
                     /gene="sarT"
                     /locus_tag="PWN45_002867"
     CDS             989..>1122
                     /gene="sarT"
                     /locus_tag="PWN45_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDL0624552.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     V"
BASE COUNT          422 a          140 c          126 g          434 t
ORIGIN      
        1 attttgatta acacaatcct ttcatctgca tgggatcgtt ctttgattag cataccttta
       61 ttatttaatc tattaactga cctaacaatt tgaggataca taaatctaat actttcaaac
      121 aaatctttta aatttagtat ctcatttttt cgagatgata ttacatataa gatagataaa
      181 tcaagaaatg ttaaattaaa tcgatatttt aaaaaatctt ttgagtacat aatgttcata
      241 aaaagtataa aaaattcttt gctatatttt ggagcccatt gatattttat gtagtctctt
      301 tccttattaa atcctttaat tattttatct aactcattaa taacacaagc aatcttttta
      361 cgttgtattg gagtaactga aacaaaaata cggcgttcat ctgcttcgtt tctagactta
      421 tttataaatc cttttttaga tagtgactta atgttcttta caacatcaga ttgtttgtaa
      481 agtatgtcac caatgatttc ttttagtgaa atactgtgtt catttttgta ataaaaatat
      541 gctaatatca acaattcttt taatgataat ttatattgtt gctttaactc ttgagtgaga
      601 aaaaatatgt acgcttctac atttatgaat ttattgacct tttcgaaagt ttgatagtcc
      661 accttacatt ctcctactat tttctgatgt cccaatactt tatattattt aaagtaatat
      721 aaaagcaatc tcttttaaag acatgttaac ctaaatattt tatatataat atatatatat
      781 aaaatataaa taactaaaaa tcacagaata acaaatgaca aattcattta ttatccaaaa
      841 ataaaaacaa ttgaacaaaa tcaaaatata gtgttatgat tcatttgtgt ttatagctag
      901 caccacaaac taaatatatt tggagttatt actagttaaa gacaaatatt atttgacgtg
      961 acaattaaaa gtaagggatg aagacttgat gaatgatttg aaaagcaaga gcaatattaa
     1021 attaatgaaa agagtattaa ctacatatga gctgcgtaaa tatttaaaaa aatatttttg
     1081 tcttacatta gataattatt tagtattagc atatttagat gt
//
LOCUS       DANJFZ010000246         1113 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.255, whole genome shotgun sequence.
ACCESSION   DANJFZ010000246 DANJFZ010000000
VERSION     DANJFZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.255"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>1113
                     /locus_tag="PWN45_002868"
     CDS             <1..>1113
                     /locus_tag="PWN45_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0624553.1"
                     /translation="TCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLG
                     QFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWG
                     NYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPI
                     VSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTV
                     NINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKS
                     NRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQ
                     SDEKVTRYFQKEDDLSYNRGLINELFIKLANEFNIE"
BASE COUNT          440 a          115 c          174 g          384 t
ORIGIN      
        1 aaacatgtaa agaatgcttg ttttatcctt atttgctagt tcaaggtgaa gctggcatag
       61 gaaaatcaca ccttttagcg catctatcta aaaaacttag agatgaaaat catattattt
      121 atctgttttt gggacaattt tttactaaaa atgaagatcc atggcatcaa atacttaatg
      181 atttagaagt tacaaattct gttgataatt ttttaaggtc aataagtaat aaggccaaag
      241 aaacaaaaaa aagagctttt attattattg atgcgcttaa tgaaggtgaa ggtaaaaggt
      301 tatggggaaa ttattttcaa agctttatca accatataaa gaagtattct aatatagctt
      361 taatattttc tattagaacc ccttttgaag atgttatatt acccaaaaac gcaatacaag
      421 ataacaatat tgtagtattt cagcatgaag gttttagtaa ggaagaaaac tataatccaa
      481 ttgtatcttt ttgtgatttt tatggattag agctacctaa gttacctata ttaaatccag
      541 aattcaacaa tccattattt ttaaaactaa tgtgtgagta ctgtgtaaat aaatttaagg
      601 aatttgacca aactataagt gttgcagagc tatttacaaa tgttcttaag acagtaaata
      661 ttaatttatc taaggaagat aaatttgatt ttgataaaaa tattaatgtt gtacaaaaag
      721 taataaaagg attagttgaa ttaatgaacg actcagaatt taatcaacta aattatgagg
      781 aatcctatac tgttgttaat aatatagcta aagagtatgt tcaaaagtca aatagatttt
      841 tagaagcgct aatagatgaa aatatattaa ttaaaaatac tggctataaa ggtgaaatga
      901 ttatttattt ttcgtatgag cgtatgggag attacttttt atcagaatac cttttagaga
      961 aatatagaaa tgttgataaa agagatttag taactaaatt acagtcagat gaaaaagtga
     1021 caaggtattt tcaaaaggaa gacgatttat cttataatag aggtcttatt aatgaacttt
     1081 ttattaaatt agcaaatgaa tttaatatag agc
//
LOCUS       DANJFZ010000247         1104 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.256, whole genome shotgun sequence.
ACCESSION   DANJFZ010000247 DANJFZ010000000
VERSION     DANJFZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.256"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..238)
                     /locus_tag="PWN45_002869"
     CDS             complement(<1..238)
                     /locus_tag="PWN45_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0624554.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTE"
     gene            329..862
                     /locus_tag="PWN45_002870"
     CDS             329..862
                     /locus_tag="PWN45_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDL0624555.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
BASE COUNT          405 a          150 c          141 g          408 t
ORIGIN      
        1 tctctgtatc tcttaatact tttttgctat acgctaagaa ttcttcgcct gctttagttg
       61 ccttagcacc tttttgattt ctaaaaagca aaactacacc taattcattt tcaagcttgc
      121 taattctttg agatatatta gattgtgcat atctaagttt aattgcagct ttattgatag
      181 atgcttcttc acatacagtt ataaaaattt ccaaatcatt aaagttcaat ttaatcacct
      241 atcacttttt atgataacta tatcacaatt ggataatatg agattttact tattattgct
      301 atccttttta taaaaggggg tatgctaaat gcatgcaatg caatatcatg ttaagttgcc
      361 tagtgattac aatatggaaa taattagaga tagagttcga ttaaatggtt ataagactga
      421 tggatttaaa aatttaatta taaaagctta tttaataagt caaacaacta ctaattgcat
      481 tacaaatact tattcaccat tatatttatg gagaagttca gaggggatga ctgaatttat
      541 attcaatgga ttttatgata atgttatttc ttcttttgga tggcaaaata taaatattgg
      601 tgtgatatat tcaatgaaca taaccgattc tgttaaacat agtctttatg cgcttgaaga
      661 atatatcgac attttcccta ctttatctct aaaagaaata gaaatcaaaa aattgtttag
      721 aatcttcgat aatgcagttg cagaaattat aatttataat cctgataagt ggaagtttgt
      781 gaaatattca tttattgaaa atcttccaaa gagtctcaat ccaaatataa aaatttatga
      841 tgtactatat atctcacaat gaaagtacag atttaaagtt attattatca acattagaaa
      901 acagactaaa ataatttatc tgaacttatt aataataaag aacctgctag atgacagcta
      961 tttaaaatac ataagctact catttagcag gttaaaatat taatcaatcc atactaacta
     1021 gtatgattca aaggttttca tctattaaat tctttactat tttgaaaatg aatgcccgca
     1081 atttgaaatt tcaactagaa ataa
//
LOCUS       DANJFZ010000248         1091 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.257, whole genome shotgun sequence.
ACCESSION   DANJFZ010000248 DANJFZ010000000
VERSION     DANJFZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.257"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            470..>1091
                     /locus_tag="PWN45_002871"
     CDS             470..>1091
                     /locus_tag="PWN45_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDL0624556.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMK"
BASE COUNT          392 a          112 c          194 g          393 t
ORIGIN      
        1 taagaataaa cgagtagtaa aaatgatcga aattggtagt atcgcatatt taaacggtgg
       61 ttcaaaaaaa tataatcata tcttaaatca ggaaaataga tagtaatgtt tgaatatacc
      121 aagtgaaaat gcccattgtg attgataaag gaaaaaactg ttttaattta aaaatgaaat
      181 atagagcgtg ttattttgta tgatagctct tataacattg gcgtttttaa tccaatagta
      241 tggacggttt tagctggttt tgttgcggaa tctgtttaga caattcttaa aaattggact
      301 tttcatacta ataaatttgg attaagagat aaaactaatt ggataagaca ttaaatagac
      361 gctgtttaaa aataccacag aaaaagctgt agatagtgtt ggaacgatag ttggtgaagg
      421 tacaaaaagt attggggatc atttaaaact aatgaaacgg gagtggtaaa tgttgctttg
      481 cgatgtcaga gtcatttata aaaatccgaa atacaaagtc attcaacata acggtgaata
      541 cttattagtc gatttagtaa gcacttggtt cgtgtacttt tttcctttca ttaattggtt
      601 cattccaaaa aagtacgcga taattagcga agaagaattt gaaaatttaa atgttgttaa
      661 accaaataaa aataatgttt tctggtcagt tataggaagt tcggttttgt ttggagttac
      721 tttaaggaaa tacatacatg tttttgatgt tcaattagat aagctagttg taatgatatt
      781 gtgtgctctc gctttaattt gtgttatagt tttttatttt aacttaaata gaaagcttaa
      841 gttaaaagtg tttgatacaa atattgaaaa aaataagaga gttatattaa taccaacgtt
      901 taaacttggc tgttttttag ttttcggata tattttcgct ggaagttttt caatattttc
      961 attaattgcc cttatgacaa tcgaacctca aaatataata atatttattt attggattat
     1021 gatgacaatg cttttctttt tgttaaatat gacttcgata ggtaatgaaa aagttcgcgt
     1081 tataatgaaa a
//
LOCUS       DANJFZ010000249         1066 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.258, whole genome shotgun sequence.
ACCESSION   DANJFZ010000249 DANJFZ010000000
VERSION     DANJFZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.258"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..235
                     /locus_tag="PWN45_002872"
     CDS             <1..235
                     /locus_tag="PWN45_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624557.1"
                     /translation="KEEELEPWTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYY
                     MYKKFGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            246..746
                     /locus_tag="PWN45_002873"
     CDS             246..746
                     /locus_tag="PWN45_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HDL0624558.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            757..>1066
                     /locus_tag="PWN45_002874"
     CDS             757..>1066
                     /locus_tag="PWN45_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624559.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTNIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEF
                     D"
BASE COUNT          417 a          102 c          229 g          318 t
ORIGIN      
        1 taaagaagaa gagcttgaac catggacatc atgcgaattt gactttacaa gagatggcaa
       61 attgaatgta tcttttgatt atattgattg ggtgaattca gaatttggac caatgggaag
      121 agaacattat tatatgtata aaaaatttgg aatttggcct gaaaaagaat atgccataaa
      181 ttgggttgaa aaaataaaag attatgttaa agagcaagat gaagctgaac tataggggag
      241 ataatatgac tttcgaagga aaactaagtc aaatgtacaa cgagattgcg aatgagatta
      301 gtgggatgat accggtagaa tgggagcaag tatttacaat agcttatgta actgatcaag
      361 ctggagaagt catctttaat tatactaaac cagatagtga tgaattaaat tattattcag
      421 acatacctaa agattgcaat gtctcaaaag atatttttaa gaattcatgg tttaaagttt
      481 atcgaatgtt tgatgagtta agagaaactt ttaaagaaga agggcttgaa ccatggacat
      541 catgcgaatt tgactttaca agagatggca aattgaatgt atcttttgat tatatagatt
      601 ggataaatac agagtttgat caattgggcc gtcaaaatta ttatatgtac aaaaaatttg
      661 gggttatacc agaaatggaa tatgaaatgg aagaagttaa agaaatcgaa caatatatta
      721 aagagcaaga agaagctgaa caatagaggt gataacatga ctttcgaaga gaaaataagc
      781 aaattatata atgagattgc gaatgagatt agcagtatga taccggtaga gtgggaaaaa
      841 gtatatacaa tggcttatat agatgatgga ggaggtgaag tattctttaa ttatactaaa
      901 ccaggtagtg atgacttgaa ttattacacc aatataccta aggagtataa catttctgtg
      961 caagtatttg atgatttatg gatggattta tatgatttat ttgaggagtt aagagattta
     1021 tttaaagaag aagatttaga accatggaca tcatgcgaat ttgatt
//
LOCUS       DANJFZ010000250         1054 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.259, whole genome shotgun sequence.
ACCESSION   DANJFZ010000250 DANJFZ010000000
VERSION     DANJFZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.259"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(61..1047)
                     /locus_tag="PWN45_002875"
     CDS             complement(61..1047)
                     /locus_tag="PWN45_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDL0624560.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT          320 a          217 c          127 g          390 t
ORIGIN      
        1 tagtgaaaat aattagaatc taactatttt catactcgtc ttaatatcat tattgaatcc
       61 ttatttatta ctatctataa tttcacttac gatatcatca tatccaggcc acgtatatcc
      121 tgcatagtca acaatcagcc aacccgttct agctttccca tttgctttaa tcgtttttgc
      181 aatttcagga tttatatgtg atgcatagtt ataagtacta ttaaatgcgc tgcctccaga
      241 cgctacactc aagaaattca catatacatt gtctttgtta ctatccgttt tagctttagc
      301 caataaattt ttaacagctt caactttttt atcatagtaa tctttatact catcttgtac
      361 ttttaaattt aagctaccat tattaatttt cgtttcaaat gtcgcattgt ctgcccattg
      421 aatacctgac gtgtcagcac cataaccact ttttatgtac gtacccccca ttctattgaa
      481 taggacaatt ttacctttcg tttcttttaa agtaggattc gcattacttc ctgtgtaaaa
      541 aagattctga tattgcgggt tattataata atattctcta aaaatttctt caaatgtctt
      601 cgtaacttta gaatcgctat cgtagtcctt tttcatagac atcacaattg tttcgtttgg
      661 ataagcactc aagtaatatt tagcatcatc gagaaatttt cctaattcat gatgcaaata
      721 aaccatgccg tgatgaactg aaatcatatt atcagcactt gctctacctc taatatcaaa
      781 aaacctgact cccgacttca tttgggtaag gtaatcttta tcttgagtct ttgcccaaac
      841 tgattttact ggatccttta aagtgaatga gccactatca tgtgaacccg gtatattaat
      901 ctcagttaaa tgttttccat catcaagttt actcatccaa ttttctggac ttttactcaa
      961 cgaatctgac gcatgtgctg aatgatacca accactcatc actactaaaa tgatacttaa
     1021 aaacaaagtc ttaatacact ttttcataat atca
//
LOCUS       DANJFZ010000251         1027 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.260, whole genome shotgun sequence.
ACCESSION   DANJFZ010000251 DANJFZ010000000
VERSION     DANJFZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.260"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..171
                     /locus_tag="PWN45_002876"
     CDS             <1..171
                     /locus_tag="PWN45_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624561.1"
                     /translation="KPGIIQMDIDSLEVINAEKSSLEKNTRDNYFIHAIDRIYINTSK
                     GLFPESELVAWG"
     gene            206..328
                     /locus_tag="PWN45_002877"
     CDS             206..328
                     /locus_tag="PWN45_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624562.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            304..402
                     /locus_tag="PWN45_002878"
                     /pseudo
     CDS             304..402
                     /locus_tag="PWN45_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            711..848
                     /locus_tag="PWN45_002879"
     CDS             711..848
                     /locus_tag="PWN45_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624563.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
BASE COUNT          417 a          116 c          165 g          329 t
ORIGIN      
        1 aagcctggta ttattcaaat ggatatagat agtcttgaag ttatcaatgc tgaaaaatct
       61 agtttagaaa aaaatacaag agataattat tttattcatg ccatagacag aatatacata
      121 aatactagta aagggctatt tcctgaatct gaactcgtag catggggata aagaaagttt
      181 actttaaaaa ttttaacctt caaatgtgaa ggttattttt tattgcccaa aacgtgctga
      241 tgacatttta aaagcaagta tggaatatca gtcgacagac tataaacgga ggtatatctc
      301 atggaaaaca atgatagcaa tgaagtagct tatttgttaa aagatggtaa atttacaaaa
      361 gtatatgtca atcaagatag tgtatctttt gtgccagggt aacaagcaac cgaattatta
      421 tttaacagta aaccgaattc aattgttatg ttacataacc atcctggaca gtcaggattt
      481 tcagaatatg atttatttac attttttaag catccatcaa taaaatcaat gacaatcgtt
      541 actaataagg gacaagtaaa atttattacc aagtcaaata ggtttcatgg taaaatagtg
      601 agtaaatttt gtgctaaata ttttacgcat attaatatca ttaatgatag ccatattgaa
      661 aaattattga aaaaacttta tagtataaat atgattaaat ataaagtgag atgattttaa
      721 ttatgttaga ttcacaatta gatggaaatg taactgcaaa agaattgagt gaatctttta
      781 aagaattagt tgaagaattt gaaagtatag aaaaagcgaa aaagaattca aattcaaaag
      841 ataaatagca tcctttttag acagataaag agaaagtggt gctattttta tacgcttttt
      901 taactattta ctgaaaatgc agaagcacaa aagttaacat atttaaaaga acttggtgaa
      961 gatggcgaat ataaatatgt tgccaaaata gatagtaaaa catctaaatt atgtcattca
     1021 cttaacg
//
LOCUS       DANJFZ010000252         1016 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.261, whole genome shotgun sequence.
ACCESSION   DANJFZ010000252 DANJFZ010000000
VERSION     DANJFZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.261"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..326
                     /locus_tag="PWN45_002880"
     CDS             <1..326
                     /locus_tag="PWN45_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624564.1"
                     /translation="IPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEFD
                     FTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKEQ
                     DEAEI"
     gene            337..825
                     /locus_tag="PWN45_002881"
     CDS             337..825
                     /locus_tag="PWN45_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HDL0624565.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT          410 a           96 c          199 g          311 t
ORIGIN      
        1 atatacctaa ggagtataac atctctgtgc aagtatttga tgatttatgg atggatttat
       61 atgatttgtt tgaggaatta agagatttat ttaaagaaga aggacttgaa ccatggacat
      121 catgtgaatt tgactttaca agcgaaggta aattaaaagt ttcatttgat tatatagatt
      181 ggataaatac agagtttgat caattaggcc gtgaaaatta ttatatgtat aaaaaatttg
      241 gggttttacc agaaatggaa tatgaaatgg aagaaattaa agaaatcgat caatatatta
      301 aagagcaaga tgaagctgaa atatagggga gataacatga ctttcgaaga aaagctaagt
      361 caaatgtaca atgaaattgc aaatgaaatc agtggaatga taccagtaga atgggaaaaa
      421 gtatatacaa ttgcctacgt agatgatgaa ggtggagagg ttgtttttaa ttatactaaa
      481 ccaggaagtg aagatttgaa ttattattca gatattccta aagattgcaa tgtctcaaaa
      541 gatattttta agaattcatg gtttaaagtt tatcgaatgt ttgatgagtt aagagaaact
      601 tttaaaaaag aagatttaga accgtggaca tcatgtgaat ttgactttac aagaaaggga
      661 aatttaaaag tatcatttga ttatatagat tggattaaat taggttttgg cccatcagga
      721 aaggaaaact actatatgta caaaaaattt ggtattttac cagatatgga atatgaaatg
      781 gaagaaattc gagcagtaga gaagtatgtt aaagagcaag agtagcagac atgttataaa
      841 agactgtgca aaatcaccct cgttttacat ttgattcaaa gaagaaggta aaagataaga
      901 ttatttgcaa cttaaaaagt caattagctt atcggtatcc atacatcatg gataaatgag
      961 ttcaactaat taacaaatca cgatataaat ttaaaattgt cattaattga aaaggt
//
LOCUS       DANJFZ010000253         1009 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.262, whole genome shotgun sequence.
ACCESSION   DANJFZ010000253 DANJFZ010000000
VERSION     DANJFZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.262"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(126..977)
                     /locus_tag="PWN45_002882"
     CDS             complement(126..977)
                     /locus_tag="PWN45_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL0624566.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          405 a          170 c          151 g          283 t
ORIGIN      
        1 ttttaatgtt agaaaaaaac taaataaaaa ctcgctaatg atatccaata atatgtatac
       61 aaaacgagac atatattgca tatgattaac gagatactga aaatatttta tcacccctaa
      121 aatgattatt cattttcagc ggtaattcga cctaaagtca aacttacaat aaaaccgatg
      181 ataaatacta ctaatgaaac gaaccacatc acgatattag ttggtaaacc tggaaatact
      241 gcaaagaggg agccaacaac aaaaccaatg attaatgcaa aagtcattag tttatgatgt
      301 gttaggaaat actggataat tttgcttgaa ataatgaatc cagcaagcac gccaaatccg
      361 actgcaagta atataggaag acctgcaaag ttaagtttaa caacttcaga tattgctagc
      421 atgaccgtac catagacgcc aaatactaat aacataaatg accctgaaat acctgggagt
      481 aacatagcac tagatgcaca catacctgca ataaaatatt taataataag actagttgat
      541 agagtaagtg tttctccagc atgtttatca ccattattca ttaatgtaat aacaattaag
      601 atagcgatac cagctataac catcatgtaa tgtttagttg taaatgacgt tttatagtta
      661 gaaattttca ataaatatgg aacgatacca atgattaatc caccaaagaa aaacatagtt
      721 ggaatatggt gttggcttaa taaataatta aaaagattac ttagtgatcc cattgccagt
      781 aacattccaa ttataatggg gattaaaaat gtaaaacttg gccaaaaacg tcgtgagaat
      841 atgccgctaa ttgaagcgat aaattgattg taaataccta acaataatgc gatagtccca
      901 ccgctaacac caggtaccaa gtcactcgtt cccatagcaa aaccttttag aatattaatc
      961 catttaaatt gttgcatgaa taactccttt caaacgattg gaataaaat
//
LOCUS       DANJFZ010000254         1007 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.263, whole genome shotgun sequence.
ACCESSION   DANJFZ010000254 DANJFZ010000000
VERSION     DANJFZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.263"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..994
                     /locus_tag="PWN45_002883"
     CDS             <1..994
                     /locus_tag="PWN45_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDL0624567.1"
                     /translation="LKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVR
                     YKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKD
                     ITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLE
                     DLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIF
                     ITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQ
                     KKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
BASE COUNT          430 a          100 c          168 g          309 t
ORIGIN      
        1 attaaaagct tatgaagaag ttgaaaatag attgggacac atgccgttac taatggattt
       61 catacaacaa cattctatag atccaagcgt tatattttct aaatttagta attattacga
      121 gttcttagtg agatataaaa aaatagatac attattgacg gaaaatgaat ctaaaaatct
      181 ggttttcttt tcaaggcaaa tagcacctgg attgaaaaga atcgatagtt tagttttaga
      241 agaactttta aaaaatgaat taacatatga tgaattaaaa aataaaatgt tgaacgaagt
      301 taaggatata acagaagatg atatagatac ttcattaaga attttagatt tttcatttta
      361 caacgcagga attgaaaaaa tctatggaag tcccattata gaacgtaatg aaagaatgat
      421 aagactaagc gatgcattta ctaatgctct aagtaatcaa acatttaata tgtttttaga
      481 agatcttatt gaattatcaa agtataacaa tgaaaaatac caaaaaggta aaaatggact
      541 gattttatat aataaatatt caagagaaga ttttagtaaa atatttaatt ggaataaaaa
      601 tggttcgtct gtaatcatgg gatacatgat aaaaagccaa gaaatgccaa tatttatcac
      661 ttatgataag catgaggata taagtgatag cacaaaatat gaagatgaat ttttaagtca
      721 agatgaatta aaatggttta ctaaatctaa tagaacgcta gaatcaaaag aagttcagaa
      781 aattttgtcc catagagcaa aaggaattaa aatgtatatt tttgtacaga aaaaagatga
      841 tgatggtata tatttttatt atttaggaac tgctggatat atagaaggtt cagaaaagca
      901 agataaaatg ccgaatggtt caaacgtagt aacgatggat ttagcactgg ataaagctgt
      961 aagagatgat atatatcgtt atctaacaaa ttaatgtaaa ccgaaaa
//
LOCUS       DANJFZ010000255          996 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.264, whole genome shotgun sequence.
ACCESSION   DANJFZ010000255 DANJFZ010000000
VERSION     DANJFZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.264"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..822)
                     /locus_tag="PWN45_002884"
     CDS             complement(<1..822)
                     /locus_tag="PWN45_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDL0624568.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVT"
BASE COUNT          316 a          196 c          129 g          355 t
ORIGIN      
        1 agtcacacca tccgttaaaa tgataattaa atcatcaagg tatattggaa tttcttgttg
       61 ttgatatcgt gtttgtgaac tgattcctaa cactctacct ctaactgaaa tttcttcaaa
      121 ttcttctttt tcagcgcgat aaatatatcc aggctcatga ccagctgaac tacaatacaa
      181 taaatggttc atttcttcat ataaaccata aaacattgtg acgaacatat tttgattaat
      241 attcttttca acaacacgat ttaaacgttt taaaccatca ctcggtaatt gtgagtgtcc
      301 ataagaatcc atgccaaact ttatcatact cattgctaaa gcagctggta taccttttcc
      361 aatgacatct gcaacagcaa agctcattgt gccatcgtta tggtcaatta aattaaaata
      421 atctccactt actttttgtg ccgccactga aataacgcca atttgaatac tatcaaattg
      481 tggaatatct gttttaagca ttgtttgttg taagctagaa gctaagtcta tctctttatc
      541 gtgaacttga agtttatcta ccaatctttg ataatctcga taactataac caaagccttt
      601 aacgatttct tgtaagacat ctaatgtctt gaacacatct tcacgcgtta agtttaacgt
      661 caatatatag tttttgtgaa tatcgacaat gtcttcaggc aagacgtcct tacgaatcac
      721 ttcgtcagtg tatttctcac acttttttat caattctact ttatcttggc acgttaaact
      781 ttcatcaatt aaacccttat aatgttgctt aaattcttcc acgattagtt gcctcctcac
      841 ttcccttaca aagcaaatgc ttaagcaagg ataattatga ttcacttact aacatttatt
      901 taattgcact tatcaatttt gtcgtttgca attttgctgt agatgatact tttaagctaa
      961 aaaaaacttt aggatggaat acctaaagcc taaaaa
//
LOCUS       DANJFZ010000256          987 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.265, whole genome shotgun sequence.
ACCESSION   DANJFZ010000256 DANJFZ010000000
VERSION     DANJFZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.265"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            581..958
                     /locus_tag="PWN45_002885"
     CDS             581..958
                     /locus_tag="PWN45_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDL0624569.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
BASE COUNT          428 a          101 c          139 g          319 t
ORIGIN      
        1 ggcaaatata ataagaaaat aattaagaat gacatgtcaa aaattaggcg taatttttag
       61 atttatctat atatttatgc caccgctcaa acttttataa aaagtaaaaa aacaatattt
      121 atattgaact tgtgacgtga atttctaaaa cacaaccaac aatccacaag gaaactagta
      181 aataagttta aataagattt cgaaaaaatg tgtaatatta taatatgaaa atctttaaga
      241 ttatagaaga atcatactga ctaaaaagat tttgaaagtg attaagtatt taaaacacgc
      301 caatcttaaa ccgctattga caaatatgaa gcggaaccac cgttgttttt cttttcaggt
      361 ccaattgaat aaatgttatg aatagaatgg ataaaaatat aattatttaa gtgttaaagt
      421 aaaccaaatc atgatttaag attataagaa agaaaaattg actaatgtta aaaccaacaa
      481 tcatatgggt tgtaacgttt atacaatcat cgtaaaactc aatatgctta atttattcaa
      541 aatgttcgtc aataagtaca atgaaaagga gattgatttt ttgaataaaa aatatcttat
      601 gattgtaatt ataattttaa tattgattct aggcggaatc tttttgaaga tgaaatatga
      661 cgaaaagaaa tattatgatg aacaaaaaga aagaataacg atttatatga agtacaatgt
      721 gaaaggttat aaaaatataa gcttcgctaa ttttaaagaa aacccaatgg atggttattc
      781 tattagtggt tatataaata atgataaaaa gttatcattt acagctggta taagatctgt
      841 tgatgatttt caatttgata ccgatatttc ttatacagat gaattgggta gaaaatttaa
      901 taaaaatcct aagtcagttt ctgaaataaa aaaagagcaa aatacgtcca ataaataatt
      961 gttcatattg tgatgaaaca aaaatat
//
LOCUS       DANJFZ010000257          906 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.266, whole genome shotgun sequence.
ACCESSION   DANJFZ010000257 DANJFZ010000000
VERSION     DANJFZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.266"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            100..837
                     /gene="sdpB"
                     /locus_tag="PWN45_002886"
     CDS             100..837
                     /gene="sdpB"
                     /locus_tag="PWN45_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDL0624570.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT          302 a          117 c          152 g          335 t
ORIGIN      
        1 ttgaatcaaa ttagtaattt gttgtctcat atacttagtt tcgtttatca tgtagaagta
       61 cctccaaaaa tcaattatta aacaggaaag gatataacag tggaaaatga aaaaaagaaa
      121 tacacgttta aagatatagc atggagagat ttgcttataa ttccaattat aggtgttata
      181 ttttatgcac tcacaattgc agcttacata ggaccaattt tgtttggtta ttatgaaatc
      241 gatagtaatc tgatatttgt agcgactatc acacaagctt tagcatatac gattgggatt
      301 ggcgcatttt atttatttca tttaaaagtg atgccatcaa ggcttcgagc tggttttgcg
      361 tatatcaaaa aatattggct tagattgtca ataacgtata ttattgctct catcttaatt
      421 tatacatatg aatatatgac acagtttcta cctaaacatt tgcaatatag tgaaacagcc
      481 aatgaacttg agctaaataa aatgtttgaa gtgcctgcat ttttacctgt agcattctta
      541 ttaattgtga ttgtaggtcc aatagttgaa gaaatcgtat ttagacatat tttaattgga
      601 gaattaggta agaagtttaa ttttatagct atgagtattg tttcggtgtt cttatttgca
      661 tttattcatg tgactgatgc aaagtcgccg tttgagtttg gtccatatct catactatcc
      721 atcatattag ttttcactta tttaaaatct ggaagaaacc taggttcaac aattgcatta
      781 catatagcaa ataattttgt ttctttcgtt atatcagtaa taagtattta tgcgtagata
      841 aagaggtcgg gacattaagt ttttaggcaa tgaaaaaagc tgatttctat taattatttg
      901 atagaa
//
LOCUS       DANJFZ010000258          902 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.267, whole genome shotgun sequence.
ACCESSION   DANJFZ010000258 DANJFZ010000000
VERSION     DANJFZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.267"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..99
                     /locus_tag="PWN45_002887"
     CDS             <1..99
                     /locus_tag="PWN45_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0624571.1"
                     /translation="VILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            332..477
                     /locus_tag="PWN45_002888"
                     /pseudo
     CDS             332..477
                     /locus_tag="PWN45_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
BASE COUNT          307 a          110 c          146 g          339 t
ORIGIN      
        1 gtgatattat cattaagtta tgagtatact cgacgtatag caacaacatc cgttgcacaa
       61 attgtagcta ttatattgtt attaattttt aatgcttaaa taagcttttc ttagacgtat
      121 gtgttctaag aaaagctttt ttgcttaaga atagcaatac gaagattcga ggcatgcttc
      181 attatagtgg taatattttt taacagcaag cggttattat tgagattagg ttttagtcag
      241 tttttattac taaatatttt ataaaaatgg tataatattt ttgagcaagt tgaattgatg
      301 gtggctatct gagtaaaggg gggtggtgcc tatggcatta cttaacactt ttgaaaggaa
      361 aagcctattg tgatatctat tgcaaacgca ttacatttaa tgttaagttt cggtatgttt
      421 atcgtcactt tcattggtat agtagtagca ataataaatt taagcaataa aaaataacca
      481 tcatttcaaa actttgacat agaatgatgg ttaaagaaaa tctcatttag tcaccgtctt
      541 tttaacgggc tcattaggcg acatgttcct gcatgtcgtc tttttacatt tttatagtaa
      601 catatctttt atttaattgt ctatttttat tagatgtgta acaaaatttt gaagtatttt
      661 ccgtatattg cctgctaaac atgtcagatt tgaaaggaaa atcatactgt ggtagaaatc
      721 aattatgatt atatggtagt acttataaat aagtgatttc tattgaatat gtaatgaagt
      781 tcatatatta atataacttt cattgatata gaagtcgcaa tgattaattt aaacaataaa
      841 aaacaaccat catttccaaa ctttgacaac gaatgatggt taaagaaaat ctcatttagt
      901 ca
//
LOCUS       DANJFZ010000259          893 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.268, whole genome shotgun sequence.
ACCESSION   DANJFZ010000259 DANJFZ010000000
VERSION     DANJFZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.268"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..261
                     /locus_tag="PWN45_002889"
     CDS             <1..261
                     /locus_tag="PWN45_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624572.1"
                     /translation="TKKEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENI
                     KSELNKVEAYYAQIETQIQQTSTEKIATEKTSYLINYMNAVA"
     gene            276..>893
                     /locus_tag="PWN45_002890"
     CDS             276..>893
                     /locus_tag="PWN45_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDL0624573.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHI"
BASE COUNT          336 a          119 c          140 g          298 t
ORIGIN      
        1 actaaaaaag aacaacttag caagttaatt gacagtctaa tgctaacatt agaaaataaa
       61 tatattgata ttgctgaagc atttcaaatt caatgtgcaa gagaaatcaa taatcaagaa
      121 attgaaaata ttaaatcaga gttgaataaa gttgaagcat attatgcaca aattgaaact
      181 caaattcaac aaacttcaac tgaaaaaata gcaacagaaa aaacatcgta tctaataaat
      241 tatatgaacg ctgtggcata gaaaggcggc gaaacatgac acacaaatat atatcaacgc
      301 aaatgttgat catttttact gcattaatga ttattgccaa tttttactac atattttttg
      361 aaaaaattgg ctttttactc gttctattat tgggatgtgt attagtttat gtaggatatc
      421 tttattttca taaaatacgt ggccttttgg cgttttggat aggcgcgcta ttaattgcat
      481 tcacattatt gtctaataag tatacaatca tcatcttgtt cgtcttttta ttattactta
      541 ttgtgcgtta tttaatacac aagtttaaac caaaaaaagt agttgcgacg gatgaggtta
      601 tgacttcacc atcttttatt aaacaaaagt ggtttggtga gcaacgtaca ccagtttatg
      661 tatataagtg ggaagatgta caaattcaac atggaattgg cgacctacat attgacttaa
      721 caaaagctgc aaatattaag gaaaataata ccattgttgt tagacacatt ttaggtaaag
      781 tgcaggttat attgccggtt aattacaata ttaatttaca tgtagctgct ttttatggaa
      841 gtacttacgt gaatgaaaaa tcatataaag ttgaaaataa caatattcat att
//
LOCUS       DANJFZ010000260          886 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.269, whole genome shotgun sequence.
ACCESSION   DANJFZ010000260 DANJFZ010000000
VERSION     DANJFZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.269"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            228..728
                     /locus_tag="PWN45_002891"
     CDS             228..728
                     /locus_tag="PWN45_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDL0624574.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            740..>886
                     /locus_tag="PWN45_002892"
     CDS             740..>886
                     /locus_tag="PWN45_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDL0624575.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          340 a           89 c          180 g          277 t
ORIGIN      
        1 tagggctatt atttacaaat aatataatac tgcaaagact cgaaattatt tagaattcca
       61 gttatcgtaa caatagttgc agttatttta gttccattag tgtatttagg tttaaaatga
      121 gtaatattaa tttgaagaag cattaagcga cgtagaggtg ttgtttagtg ctttttattt
      181 aacggaaatg aaaccaatat ctattttgaa aatatagggg cgataacatg actttcgaag
      241 aaaaactaag tgaaatatac aacaagattg cgaatgagat tagtgggatg ataccagtag
      301 aatgggacca agtatttacg atagcttatg taaatgatag aggtggagag atcgttttta
      361 attatactaa accaggtagc gatgaattga attattacac atatatccct agagagtata
      421 atgtctctga aaaagtattt tatgatttgt ggacggattt atatagattg tttaagaagt
      481 taagaaacgc atttaaagaa gaagatttag aaccatggac atcatgtgaa tttgacttta
      541 caagagatgg taaattgaat gttgtatttg attatgttga ttggatgaat tcagaatttg
      601 gtccaatagc aaaagaaaat tattatatgt acaaaaagtt tggtgtttta ccagaaacgg
      661 aatatgaaat taataaagtt aaagaaatcg atcaatatgt taaagagcaa gatgaagctg
      721 aactataggg ggagataaca tgactttcga agagaaacta agtcaaatgt acaataaaat
      781 tgcaagtgag atcagtggaa tgataccagt agaatgggag caagtattta caatagctta
      841 tgtaactgat caagctgggg aagtcatttt taattatact aaacca
//
LOCUS       DANJFZ010000261          876 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.270, whole genome shotgun sequence.
ACCESSION   DANJFZ010000261 DANJFZ010000000
VERSION     DANJFZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.270"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            494..876
                     /locus_tag="PWN45_002893"
                     /pseudo
     CDS             494..876
                     /locus_tag="PWN45_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
BASE COUNT          366 a           80 c          155 g          275 t
ORIGIN      
        1 aagataggtg atttctttaa aaaaatggat atattgataa gtaaacagaa aatgaaaatt
       61 gaaatattag aaaaagagaa acaatccttt ttacaaaaaa tgttcttata actttgataa
      121 atacatagat tgcataagaa taaaatttgt ataatttaac ataaaagttg taaaagtaaa
      181 gtgaattaaa aacgaacatt aaatttaggc actgtgaaag cgcagtgtct tttttgtgtc
      241 gaaattgtgt acagaataag tagttaaata aagattaagt tgagataaag tgttattcgt
      301 aaataaaaga gagtagatcg ataggaattg aatgatatta gttaactatt tattaaatta
      361 cttaataatg attaattttt agttaaagta agtttaatgt gaagcacgac cattgctcat
      421 tataatgaat gaggattgtt cgtattgcgt aatagaataa atcaaataga ctaaaaattg
      481 ggagcataga attatgaaat taaaaaatat tgctaaagca agtttagcac tagggatttt
      541 aacaacaggg atgattacaa ctactgctca gccagtaaaa gcaagtacat tagaggttag
      601 atcacaagct actcaagact tgagtgaata ttataataga ccgttctttg agtatacaaa
      661 tcagtcagga tataaagagg aaggaaaagt gacgtttact cctaattatc aacttataga
      721 tgtaacttta actgggaatg aaaagcaaaa ttttggtgaa gatatttcta atgtagatat
      781 atttgttgta agagaaaatt ctgatagatc tggtaacttc aattggtggt attactaaaa
      841 caaacggttc aaattatatt gataaagtaa aagatg
//
LOCUS       DANJFZ010000262          838 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.271, whole genome shotgun sequence.
ACCESSION   DANJFZ010000262 DANJFZ010000000
VERSION     DANJFZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.271"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..282
                     /locus_tag="PWN45_002894"
     CDS             <1..282
                     /locus_tag="PWN45_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease
                     C-terminal domain-containing protein"
                     /protein_id="HDL0624576.1"
                     /translation="ITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDG
                     LIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS"
     gene            304..822
                     /locus_tag="PWN45_002895"
     CDS             304..822
                     /locus_tag="PWN45_002895"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL0624577.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT          319 a          101 c          164 g          254 t
ORIGIN      
        1 ataactaaac aagaaacaaa tatccagaaa gagggtatgt tattgtgtca acatttaagt
       61 gtgaaaccag atacacttaa attcatgttg aaagttttct tagacttaaa atttgtaaca
      121 caagaagatg gtttaattcg aatcaatcaa caacctgata aaagatcgat tgattccagc
      181 aaagtatatc aattaagaca acaacgtatg gatgttgaaa agcaattatt atatcaagat
      241 ttttcagaaa taaaaaattg gataaagtca caattgtcgt gagcaattta ggaggaaata
      301 ttaatggatt taaagcaata cgtatcagaa gttcaagatt ggccgaaacc aggtgttagt
      361 ttcaaggata ttactacaat tatggataat ggtgaagcat atggctatgc aacagataaa
      421 attgtagaat acgcaaaaga cagagatgtt gatatcgttg taggacctga agcgcgtggc
      481 tttatcattg gctgtcctgt agcttattca atggggattg gctttgcacc tgttagaaaa
      541 gaagggaaat tacctcgtga agtcattcgt tatgagtatg acctagaata tggtacaaat
      601 gttttaacaa tgcacaaaga tgcaattaaa ccaggtcaac gtgtgttaat tacagatgat
      661 ttattagcta ctggtggtac gattgaagca gcaataaaat tagttgaaaa attaggcggt
      721 atcgtagtag gtattgcatt tataattgaa ttgaaatatt taaatggtat tgaaaaaatt
      781 aaagattacg atgttatgag tttaatctca tacgacgaat aataaataat ataatttt
//
LOCUS       DANJFZ010000263          830 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.272, whole genome shotgun sequence.
ACCESSION   DANJFZ010000263 DANJFZ010000000
VERSION     DANJFZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.272"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..129
                     /locus_tag="PWN45_002896"
     CDS             <1..129
                     /locus_tag="PWN45_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HDL0624578.1"
                     /translation="YVLKEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            122..823
                     /locus_tag="PWN45_002897"
     CDS             122..823
                     /locus_tag="PWN45_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624579.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
BASE COUNT          283 a          118 c          149 g          280 t
ORIGIN      
        1 tatgttttaa aagaaggtca aatcattgaa cgaggtaata ttaatcattt caagtatgag
       61 catttgcatc cgtatactga acgtctaatt aaatatagaa cacaattaaa gagggattac
      121 tatgattgag ttaaaacatg tgacttttgg ttataataaa aagcagatgg tgctacaaga
      181 tatcaatatt actatacctg atggagaaaa tgttggtatt ttaggcgaaa gtggctgtgg
      241 taaaagtacg ctcgcttcat tggttcttgg cttgtttaaa cctgttaaag gagagattta
      301 cttaagtgac aatgctgtgt taccgatttt ccaacaccct ttaactagct ttaaccctga
      361 ttggacgatt gagacctcat taaaagaagc gttatattat tacagaggtc taactgataa
      421 tactgctcag gatcaattat tattacaaca tttatctact tttgagttaa acgcgcaatt
      481 attgactaaa ttaccaagcg aagtgagtgg cggacaatta caaagattta atgtcatgcg
      541 ttcgttatta gcacagcctc gcgttttaat atgtgatgag ataacttcaa atttagatgt
      601 tatagctgaa caaaatgtaa tcaatatatt aaaagcgcaa acgattacga acttaaatca
      661 ttttatcgtt atttctcatg atttatccgt gttacaacgc ttagttaata gaattatcgt
      721 tcttaaggat ggcatgatag tcgatgattt tgcaatagag gaattattta atgttgatag
      781 acacccttat acaaaagaat tagtgcaagc attttcatat tagttattta
//
LOCUS       DANJFZ010000264          734 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.273, whole genome shotgun sequence.
ACCESSION   DANJFZ010000264 DANJFZ010000000
VERSION     DANJFZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.273"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            128..232
                     /locus_tag="PWN45_002898"
     CDS             128..232
                     /locus_tag="PWN45_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624580.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(223..295)
                     /locus_tag="PWN45_002899"
     tRNA            complement(223..295)
                     /locus_tag="PWN45_002899"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Lys,seq:ttt)
     gene            complement(299..373)
                     /locus_tag="PWN45_002900"
     tRNA            complement(299..373)
                     /locus_tag="PWN45_002900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(339..341),aa:Gln,seq:ttg)
     gene            complement(383..466)
                     /locus_tag="PWN45_002901"
     tRNA            complement(383..466)
                     /locus_tag="PWN45_002901"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Tyr,seq:gta)
     gene            complement(496..571)
                     /locus_tag="PWN45_002902"
     tRNA            complement(496..571)
                     /locus_tag="PWN45_002902"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(536..538),aa:Val,seq:tac)
     gene            complement(579..650)
                     /locus_tag="PWN45_002903"
     tRNA            complement(579..650)
                     /locus_tag="PWN45_002903"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Glu,seq:ttc)
     gene            complement(652..726)
                     /locus_tag="PWN45_002904"
     tRNA            complement(652..726)
                     /locus_tag="PWN45_002904"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:Asn,seq:gtt)
BASE COUNT          227 a          171 c          162 g          174 t
ORIGIN      
        1 tggttacaga aatttcaaca actttaaagc acgtataatg atgattttca gcttgtacaa
       61 aggagaaaaa aagaagacaa ccaagcccaa taatggactg gccgcctaat aataaaagct
      121 ctaaaagttg tattttaaaa atagttcttt aaattatata cccaccacat ttggtggaga
      181 acctaaaaaa aagcacttcc caaaaatgga aagtgcaagt agtgagccat agaggattcg
      241 aacctctgac cctctgatta aaagtcagat gctctaccaa ctgagctaat ggctctaatg
      301 gctgggctag ctggattcga accaacgagt gacggagtca aagtccgttg ccttaccgct
      361 tggctatagc ccaatatata gatggtggag gggggcagat tcgaactgcc gaacccgaag
      421 gagcggattt acagtccgcc gcgtttagcc acttcgctac ccctccagct tattcatata
      481 atttaataat caaaatggtg gagaatgacg ggttcgaacc gccgaccctc tgcttgtaag
      541 gcagatgctc tcccagctga gctaattctc caaaataatg actcctacgg gactcgaacc
      601 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaaggagcc atggctccac
      661 aggtaggact cgaacctacg accgatcggt taacagccga tagctctacc actgagctac
      721 tgtggattaa tatt
//
LOCUS       DANJFZ010000265          684 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.274, whole genome shotgun sequence.
ACCESSION   DANJFZ010000265 DANJFZ010000000
VERSION     DANJFZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.274"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            197..447
                     /locus_tag="PWN45_002905"
                     /pseudo
     CDS             197..447
                     /locus_tag="PWN45_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          271 a           89 c           83 g          241 t
ORIGIN      
        1 ttataccaag cttcaaactt aaatgtcata acaacattca tttcttaatt ccgattagat
       61 ttgtcgatta tatttacagc atctttatac tcaaaaaaca tttacttaaa aatataaatt
      121 cgatttaata attaatttaa atttagttaa tcaattttgc atctattttg ttgtaagcta
      181 tataaaagga gtgataatga tggtgaaaaa aacaaaatcc aattcactaa aaaaagttgc
      241 aacacttgca ttagcaaatt tattattagt tggtgcactt actgacaata gtgccaaagc
      301 cgaatctaag aaagatgata ctgatttgaa gttagttagt cataacgttt atatgttatc
      361 gaccgttttg tatccaaact ggagactttt aacataaaat tacttatcat tcaaaaagta
      421 aaacagcata atatcaaggt ttataacttt atcattatca ataatacctc atataaaata
      481 aaattttagg gactttttag ggactttaaa tttaaaatta caagtttaat agaaacatca
      541 aaataatcac atgtttgtgt ggaatgtaca ccccaaaagc tagactgaaa aatctatttt
      601 ttgaggtgta tttttatagg taaatataat aaattagagt agacaactca gaattccaat
      661 tttataataa tattgcttga cata
//
LOCUS       DANJFZ010000266          629 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.275, whole genome shotgun sequence.
ACCESSION   DANJFZ010000266 DANJFZ010000000
VERSION     DANJFZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.275"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            1..267
                     /locus_tag="PWN45_002906"
                     /pseudo
     CDS             1..267
                     /locus_tag="PWN45_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          264 a           71 c          110 g          184 t
ORIGIN      
        1 ttattcgttg aaaaagaaga gtcacaaaag ttaacatatt taaaagaact tggtgaagat
       61 ggcgaatata aatatgttgc caaaatagat agtaaaacat ctaaattatg tcattcactc
      121 aacggaaaaa tatttaaagt taaagatatg ataccaggtg tgaatgcgcc acctatgcat
      181 ccttggtgta gaagtaccac agtgccacat gtcggcaatt ggcgagacaa gttctttaaa
      241 gagcgtgaag gtaaatatca agtagaaggt tgttttattg aatcaggtgc acttaataat
      301 aaaagtgatg aatacggtat caaaaggaac agacatgctc aaatttatta taattcagtt
      361 agaaatcgag ataaacagat agagatttct aaaattgcta aaaatactaa tataaataaa
      421 aatttaattc aaagagttta tgaacatata tttgaaaata agtatttatt agaaagtgga
      481 tttaaacaat ttgatcctga cttttatatg gctcaaagtt ggcaacgttt aagagaaggg
      541 aaaaatatta aaaaaatgga tataataatg tagaaacatg aagctcttga acactatcta
      601 atgaataagt ataatttaca ctatataga
//
LOCUS       DANJFZ010000267          619 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.276, whole genome shotgun sequence.
ACCESSION   DANJFZ010000267 DANJFZ010000000
VERSION     DANJFZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.276"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(338..>619)
                     /locus_tag="PWN45_002907"
     CDS             complement(338..>619)
                     /locus_tag="PWN45_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0624581.1"
                     /translation="KVTLYSNGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEID
                     SLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYYTYNHIMHH"
BASE COUNT          213 a           98 c           74 g          234 t
ORIGIN      
        1 aactctatga taacaaaaat actgtttgtg tataacaata aattgagata ctcaataatt
       61 tgataatgtg aaattgatac ttttcaaaat agttgaatat gacaattaat gattagtttt
      121 gcaatggtgc tataatcaat atttagcagt tatcattatg cttgaaaggg tggtaaaggc
      181 ggagaagtca tccacttttt cctatactac aaggaccttt actttaaaac aacgaattta
      241 caaaataaaa ggcataattt cttttctgtc acatcgctca ttctaactac tagactttta
      301 aaatcgttcc tgtaatttaa actcaatcaa ctttcaatta atgatgcata atatgattat
      361 aagtatagta gttaacttca aatatcttag aaatgtcctg tactatatct cttttattct
      421 caaaagtcat aggtaataaa gcctttcctt ttccttgttt aactaagttt attattgctg
      481 ccaatgaatc aatttccatt acccgttgtg aatcaagctt attatctaaa gttattttcc
      541 ttaaaggaca aaactcatct ttatttatta gtaaagcctc tttatcataa ttacttccat
      601 tactgtaaag cgttacttt
//
LOCUS       DANJFZ010000268          612 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.277, whole genome shotgun sequence.
ACCESSION   DANJFZ010000268 DANJFZ010000000
VERSION     DANJFZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.277"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..362
                     /gene="isaB"
                     /locus_tag="PWN45_002908"
     CDS             <1..362
                     /gene="isaB"
                     /locus_tag="PWN45_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDL0624582.1"
                     /translation="DKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGN
                     EASQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDH
                     NRVVEVTVGHTPYKTSK"
BASE COUNT          249 a           63 c          100 g          200 t
ORIGIN      
        1 tggataagaa ttttattaac gcgattaagt atgataatgt gaaattcaat ggtattaaat
       61 tagctaaaac gaatacgata aaaaaagtag agaaatatga tcaaactttt aaaggtgttt
      121 ctgcaaaagg aaacgaagca agtcaattgc aatttgtagt taaaaataat atttcattaa
      181 aagatatcca aaaagcttat ggcaaggact tgaaaaaaga aaatggtaaa acaaaggaag
      241 ctgatagcgg tattttttac tatcaaaatg ctaaaaagac attaggcatc tggtttgtcg
      301 ttgatcataa tagagttgtc gaagtaacag ttggacatac accatacaaa acaagtaaat
      361 aaaataatgg catattaagg ctagagtgtg aggagtgata ccgcattcta gtctttttta
      421 ttaaataata acgattattg cgtcttacat agttgtttga aattgataaa aatgtttatt
      481 tgattatgat tatttaaata gttatctcac atatttcaag cattgattaa gtatatagag
      541 cactattttg tatttgttaa tattttcaca aaaataaagc cttgataaat tttaaaatat
      601 aattaagctc aa
//
LOCUS       DANJFZ010000269          582 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.278, whole genome shotgun sequence.
ACCESSION   DANJFZ010000269 DANJFZ010000000
VERSION     DANJFZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.278"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            161..>582
                     /locus_tag="PWN45_002909"
     CDS             161..>582
                     /locus_tag="PWN45_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0624583.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFN"
BASE COUNT          216 a           94 c           88 g          184 t
ORIGIN      
        1 tacattttat gaaaaaatcc atttttatta caattcaaca ctttatatga caacttcatt
       61 acagttactt ttattgttga ttgcttacat tgttttctaa aaaaaatttg ttatcataat
      121 taacgttgaa taaagaaaaa aattaacttg ggagataaaa atggaatata aaaagatact
      181 aattcgttta ttaattgctt ttgcagtact tttctcagca gatttcactt atcaatccgt
      241 cgaacaaacg catcaatcgc atgccgcagt taattattat agtaaaaacc aatgtacatg
      301 gtgggcattt aaacgtcgcg cacaagtcgg taaacctgtt tctaatagat ggggcaatgc
      361 taaaaattgg tattacaatg cacgtaaatc aaaatatgcg actggtcgta caccaagaaa
      421 atttgctgtc atgcaatcaa ctgcaggata ttatggacat gtcgcagttg ttgaacaagt
      481 atataaaaac ggtagtatta aagtttcaga atacaacttt tatcgcccat taaaatacaa
      541 tacacgtgta ctaagcaaaa aggcagcacg taactttaac ta
//
LOCUS       DANJFZ010000270          566 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.279, whole genome shotgun sequence.
ACCESSION   DANJFZ010000270 DANJFZ010000000
VERSION     DANJFZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.279"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            146..352
                     /locus_tag="PWN45_002910"
     CDS             146..352
                     /locus_tag="PWN45_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624584.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          245 a           62 c           83 g          176 t
ORIGIN      
        1 caatatataa aaaaaacgcc aatactatta ggtattagtt tatgaattaa ctagtttggc
       61 aactagaaaa aaacaatgta ttgctaaacg tagttactgc attaggatta ttataattat
      121 aaatagaaag agagtgtgat taataatgaa tgagattgaa actattataa gtgaaataga
      181 aaagttatta actaacaata caccatatag tatttcaaaa aactcaggtg taccacgtca
      241 aacagttact gatttaaagg taggtaaaac taaaataaaa gaagctaaat ttaaaacgat
      301 aatcaagtta tatgaatatc aaagaacatt agaaaataaa acagaatgtt aacaaaaata
      361 ttttgttagg ttaggtggac aagaaattaa atttctaatg atttcatggt tgtctgtcca
      421 cgttttgtcc acctcaataa gataatctct tagttttaga aattgtacat ggttaaataa
      481 tcaaaaatgc atattatgaa attatcattg ccattaaatc ttgaatgatc tttaagcgat
      541 gatataaaag gctgaacaca gtaaat
//
LOCUS       DANJFZ010000271          560 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.280, whole genome shotgun sequence.
ACCESSION   DANJFZ010000271 DANJFZ010000000
VERSION     DANJFZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.280"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..484
                     /locus_tag="PWN45_002911"
     CDS             <1..484
                     /locus_tag="PWN45_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0624585.1"
                     /translation="LVEPKPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCF
                     DLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGG
                     GYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA
                     "
BASE COUNT          219 a           61 c          110 g          170 t
ORIGIN      
        1 attagtagaa ccaaaaccaa aaacagtgtc ttcaaataat aaaagtaata gtaatagcag
       61 tacattaaat tatttgaaaa cattagagaa tagaggatgg gatttcgacg gtagttatgg
      121 atggcaatgt ttcgatttag ttaatgtata ttggaatcat ctttatggtc atggattaaa
      181 aggatatgga gctaaagata taccatatgc aaataatttt aatagtgaag ctaaaattta
      241 tcacaacaca ccaactttca aagctgaacc tggggactta gtggttttta gtggaagatt
      301 tggtggagga tatggtcata cagctattgt cttaaatggt gattatgatg gaaaattaat
      361 gaagttccaa agtttagatc aaaactggaa taatggtgga tggcgtaaag cagaggttgc
      421 acataaagtt gttcataatt atgaaaatga tatgattttt attagaccat ttaaaaaagc
      481 ataatttaaa tcgaaggcag gacattgaaa tatgaaattt tcaactttaa gtgaagaaga
      541 atttaccaac tacaccaaaa
//
LOCUS       DANJFZ010000272          555 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.281, whole genome shotgun sequence.
ACCESSION   DANJFZ010000272 DANJFZ010000000
VERSION     DANJFZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.281"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>555
                     /locus_tag="PWN45_002912"
     CDS             <1..>555
                     /locus_tag="PWN45_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HDL0624586.1"
                     /translation="LLRSLSDLNEHQKMDRVSFIKKHLNSNFDYLLDDTEFRSINKIY
                     SNLNIMTHINSQNNNFKRNPFINEEQLKSLLAIKKVDAHMSYDSNITSKALAEINKTQ
                     KRLVTKIDTLYNQSKKENEYVRLGEKISYTKLENHWKYLYNEIQFYNLNNQLISYVAL
                     EQEYAWAFLNELFYLIELYLKAFKG"
BASE COUNT          240 a           55 c           82 g          178 t
ORIGIN      
        1 ttattaagaa gtttaagcga tttaaatgaa catcaaaaga tggatagagt ttcatttata
       61 aaaaaacatt taaactcaaa tttcgattat ttattagatg atactgagtt tagaagtatc
      121 aataagattt acagtaattt aaatatcatg acgcatatca atagtcaaaa taataatttt
      181 aaacgtaatc catttataaa tgaagaacaa ttgaaatcac tactcgcgat aaagaaagta
      241 gatgcacata tgagttatga ttcaaatatt acttcaaaag cattggcaga aataaacaaa
      301 acgcaaaaaa gattagttac aaagatagac acactctata atcagtcaaa aaaagaaaat
      361 gaatatgtac ggttaggaga aaaaatatcg tatacaaaat tggagaatca ttggaaatat
      421 ttatataatg agattcagtt ttataatctt aataatcaat tgattagtta tgttgcatta
      481 gaacaggaat atgcttgggc ttttttaaat gaactatttt atttgataga attgtatttg
      541 aaggcattta aagga
//
LOCUS       DANJFZ010000273          532 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.282, whole genome shotgun sequence.
ACCESSION   DANJFZ010000273 DANJFZ010000000
VERSION     DANJFZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.282"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
BASE COUNT          168 a           80 c           92 g          192 t
ORIGIN      
        1 ggcagaggcc atatatctgg ctgttggtct cacaggagac atcttccttg tcatcactca
       61 tatacatata tcttgataac atagagttgt tatagtcgct acgccactca tactagttac
      121 tgggtggttg tttttatgtt atattataaa tgatcaaacc acaccaccta ttaatttagg
      181 agtgtggttg ttttcaaggg caaaaaaagg gcataatttt gaaacaaagg gcaagggtat
      241 gaataataaa ttatcttgaa aattttataa atgcatatat aacaatgttt tgattgccct
      301 tgaacttcct tgataatctg gctaattatt cctgctaaat aaatcatatt aatggctcag
      361 taacgtgatg ttgctgggct ttttaattta aacaggtatt tatatgatat ttaggaatga
      421 gatgctatta cggataaagt aaaatcctat atgcatgcgt gctactcatt tattcttatt
      481 tttaatttca catggctagt gactaataca tcataaatca ttagtgattc tt
//
LOCUS       DANJFZ010000274          525 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.283, whole genome shotgun sequence.
ACCESSION   DANJFZ010000274 DANJFZ010000000
VERSION     DANJFZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.283"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>525
                     /locus_tag="PWN45_002913"
     CDS             <1..>525
                     /locus_tag="PWN45_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0624587.1"
                     /translation="DLTLYQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYL
                     RLSKDENIFPMNFIFNETPAPYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKK
                     LNETIQFQVEYIMALRANSAKERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLS
                     GLNRKTTGEIIREL"
BASE COUNT          215 a           61 c           74 g          175 t
ORIGIN      
        1 ggatttaaca ctttatcaat ataaaaaagg acaagtcata tatcattcaa ctgatcaaat
       61 aaaatttgta tactttttag taaatggttg tattttacat gaatcttcta atattactgg
      121 tgacaattat ttaagattaa gtaaagacga aaatatattt ccaatgaact tcatatttaa
      181 tgaaacccct gcaccatatg aaatatgtac agctttgaca gattgtaaaa tattaacttt
      241 accgaaagat ttacttgagt atttatgtag aaagcataat gaaatatttg aaagtctctt
      301 caagaaactt aatgagacta ttcaatttca agtagaatat attatggcgt taagagctaa
      361 ttcagctaaa gaaagaattg aaagaatact acaaatttta tgcctttcaa ttggggatga
      421 taatggagaa ttctatgaat taaaacaaat tatgactgtt caattaataa gtaatttatc
      481 tggacttaac agaaaaacta ctggtgaaat aatcagagaa ttaaa
//
LOCUS       DANJFZ010000275          511 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.284, whole genome shotgun sequence.
ACCESSION   DANJFZ010000275 DANJFZ010000000
VERSION     DANJFZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.284"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            16..>511
                     /locus_tag="PWN45_002914"
     CDS             16..>511
                     /locus_tag="PWN45_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL0624588.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELL"
BASE COUNT          197 a           59 c           89 g          166 t
ORIGIN      
        1 aagaagggaa aacgcatgaa tcatgataaa tcaccaataa ttttaattgg tttcatgggt
       61 accggtaaat ctacgattgg taaatacgtt gcagatgagc aaaatttatc atttattgat
      121 atagattcat atatcgaaga gaagtataag ttaacaatac cagaaatatt ttgtaaacat
      181 ggtgaacaat atttcaggaa tttagagttc acatgtttgc aagaatgtat taacactgca
      241 gatataattg ctactggtgg tggtattatt gagagtgaag aggcatttaa ttttttgaaa
      301 aatcaaaaaa acattatttg gttagattgt aatattgata ttatatatag tcgaatcaat
      361 gatgacccac atcgacctaa tgcaaataat aagacaatca agcagttaaa tgacttgtat
      421 tgctcgcgga atttaagata taatgaaatc gcattcaaga aatttgatag tcatttgcta
      481 tcaatttcag aaatatatta tgaattgcta a
//
LOCUS       DANJFZ010000276          505 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.285, whole genome shotgun sequence.
ACCESSION   DANJFZ010000276 DANJFZ010000000
VERSION     DANJFZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.285"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..153)
                     /locus_tag="PWN45_002915"
     CDS             complement(<1..153)
                     /locus_tag="PWN45_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624589.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNEKT"
     gene            complement(110..256)
                     /locus_tag="PWN45_002916"
     CDS             complement(110..256)
                     /locus_tag="PWN45_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624590.1"
                     /translation="MKLFYIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKN
                     DRVE"
BASE COUNT          180 a           80 c           42 g          203 t
ORIGIN      
        1 ggttttctca tttcttaaat cttgtactgt ttgatatgga actcctgaat ttttcgaaat
       61 tttatagcct gattcagact caaacaaaga ttgaagagag tttataatat tattcaactc
      121 tgtcattttt aatccccttt cttgaattaa caatatacaa tgttgttatt aaaacagtca
      181 gtgtatggat gatttcattt cctaaaaata tattcagcca tataataata agaaatacga
      241 tataaaatag tttcatttta atcccctatc atatttttat taatcaaatt tcaataagat
      301 tcataattaa aagctaacct atactcaaaa ttttcttata atgcctctaa ctaaaaacct
      361 acattttatt ttacatataa tattttacac tatattaaaa atacatacga tactttttcg
      421 ttatctatta gatatttcta tgtaatggca aaatttattc ccgacgaaat tccaaacata
      481 attaataccc tttttctcta tttct
//
LOCUS       DANJFZ010000277          480 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.286, whole genome shotgun sequence.
ACCESSION   DANJFZ010000277 DANJFZ010000000
VERSION     DANJFZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.286"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..377
                     /locus_tag="PWN45_002917"
     CDS             <1..377
                     /locus_tag="PWN45_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HDL0624591.1"
                     /translation="GKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYK
                     RITLIEESIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREK
                     TKHNNIEYNVSLFFKALDALKK"
BASE COUNT          205 a           44 c           70 g          161 t
ORIGIN      
        1 ttgggaaaaa attaaacgaa tttattcaat catatgtcat tattttactt atagatatac
       61 gattacctgt tgttagatta tatattatta gagacattgt agatacatgt gaaggagaaa
      121 aagataatta caaaagaatt acactgatag aagaatctat taaaaagtat agatacgtaa
      181 aagatcagat taaaagattt aaaaatggat tagaagaatc gttattaaat gcaactttat
      241 ctattgaaca aaatatgttg aaagttaaaa ttgattatta taaagcctac tgctttccta
      301 gagaaaaaac aaagcataac aatattgagt acaatgtatc gttgtttttt aaagcattag
      361 atgccttaaa gaaatagttt ttcactgaaa atatttatag atataattaa aattaattaa
      421 tattaagcca ggataatgta gtcttaatcg ttctgaaata cgaaaaatgt tgtggaaatc
//
LOCUS       DANJFZ010000278          470 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.287, whole genome shotgun sequence.
ACCESSION   DANJFZ010000278 DANJFZ010000000
VERSION     DANJFZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.287"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>470
                     /locus_tag="PWN45_002918"
     CDS             <1..>470
                     /locus_tag="PWN45_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDL0624592.1"
                     /translation="IDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSE
                     SYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQKIS
                     EFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSNMILLLDDM"
BASE COUNT          196 a           57 c           69 g          148 t
ORIGIN      
        1 gaattgattt tgcagaaaga gcaatcaaaa atgaagacat agttaataaa aatgttaatt
       61 tttttcaaca aaacaggctt aaagagattg ttaattatag aaggaatagt gtagattacg
      121 ttagaccttc agaatcttat atatcagtcc aaggacatcc acagaatcct caaatttttg
      181 gaaaaacaat gacttgtggt acaagtattt cattgcgtgt accgaataga aagcagcaat
      241 tcatagataa aattagtgtg ataatcaaag aaataaacgc tattattaat cttcctcaaa
      301 aaattagtga atttcctaga atagtaactt taaaagactt gaataaaata gaagtattag
      361 atactttatt gctaaaaaaa ctatcgaatt cttcaactac agaaaatata tctatagata
      421 tatcaagatt tttagaacta agtaatatga tactcttgtt agatgatatg
//
LOCUS       DANJFZ010000279          465 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.288, whole genome shotgun sequence.
ACCESSION   DANJFZ010000279 DANJFZ010000000
VERSION     DANJFZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.288"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(84..>465)
                     /gene="icaR"
                     /locus_tag="PWN45_002919"
     CDS             complement(84..>465)
                     /gene="icaR"
                     /locus_tag="PWN45_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDL0624593.1"
                     /translation="NIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNTPE
                     EFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQKF
                     GAVEESKSQFKDEVYSLLNIFLKK"
BASE COUNT          165 a           70 c           47 g          183 t
ORIGIN      
        1 gtcaacttta tatagattta aaaaaataat ctcatgtctt tttttacaaa agtaagttaa
       61 ttattacaaa ctagtaacaa aaattatttc ttcaaaaata tatttagtag cgaatacact
      121 tcatctttga attgactttt actttcttcc actgctccaa atttttgcga aaaggatgct
      181 ttcaaatacc aactttcaag aaacagcaat attaaattct gaaagtcttc ttttgtcatc
      241 tttatctttg attcatcata gaattttgct atctctttac ttaatgattg atttaaatct
      301 tgtatttgtc cgtaaatatt tccagaaaat tcctcaggcg tattagataa ttgaacgtac
      361 attctaatat acctttcttc gatgtcgaaa ataaactcaa ataagaattg atataaagca
      421 tcaattgaat agttcgattt attttgattc atcataataa tatta
//
LOCUS       DANJFZ010000280          437 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.289, whole genome shotgun sequence.
ACCESSION   DANJFZ010000280 DANJFZ010000000
VERSION     DANJFZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.289"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
BASE COUNT          116 a           93 c           58 g          170 t
ORIGIN      
        1 tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct cttctctatt
       61 tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca aaattaaagt
      121 tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca gttctactct
      181 agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg tgacaatcgc
      241 ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa actcgttgcg
      301 ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt tttctttgtg
      361 tttgcttttt attttgacgt tttagacata aaaaaaagag accttgcggt ctcaatgcgg
      421 ctcatcgcat ccatttt
//
LOCUS       DANJFZ010000281          421 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.290, whole genome shotgun sequence.
ACCESSION   DANJFZ010000281 DANJFZ010000000
VERSION     DANJFZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.290"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>421
                     /locus_tag="PWN45_002920"
     CDS             <1..>421
                     /locus_tag="PWN45_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDL0624594.1"
                     /translation="KIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEGT
                     KYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTSG
                     LLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNL"
BASE COUNT          145 a           58 c           84 g          134 t
ORIGIN      
        1 aaaataaaaa ctgaatttaa agggaacaat ataccatatg aatacgcagc aggtgcagat
       61 gtgagtgatt ctattaacgg gaatccaatt aagtcatttc catttgaagt aattgaatta
      121 ccggaaggga ctaaatatct tgcttggtct ttaattgact atgatgcaat tcctgtatgt
      181 ggctttgctt ggattcattg gagtgtagct aatgtaagtg ttagtggcaa ttcaatttct
      241 ataaaagcag atttatcaag aacaaagggc gactatgtac aaggtaaaaa tagctttact
      301 agtgggttgt tggctgaaga tttttcagaa atagaaaatc actatgtagg acctacacca
      361 cctgatcaag atcatcaata tgaattaaca gtttatgcgt tagatcattc tttaaatttg
      421 a
//
LOCUS       DANJFZ010000282          409 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.291, whole genome shotgun sequence.
ACCESSION   DANJFZ010000282 DANJFZ010000000
VERSION     DANJFZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.291"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
BASE COUNT          174 a           52 c           59 g          124 t
ORIGIN      
        1 gcctatttaa aagacaatct ccattagaac taagatatgc atcccgaaag ttagactaaa
       61 aaactaactt tatgggatgt atttttatgc taatcatcat aaattcgaga ttaagttaaa
      121 ggtagttcaa gagtaattaa acaataaatt aaaaatagta ggatacttac tttgagggaa
      181 gaaaattaac tgtatatatt tagtttagga acaagtatta cggtttatcc tgatacaatt
      241 attgtggatg ggatgatatt tttaggttta aaatacgaca ccagcaaaca taataactgt
      301 aatagctcat aaatctcccc atatagctaa tctaaaaaaa taatacatca ttggaattaa
      361 gccccaagca tgtaaatatt aaaaatcaaa aaagatatat gtaaaaaag
//
LOCUS       DANJFZ010000283          395 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.292, whole genome shotgun sequence.
ACCESSION   DANJFZ010000283 DANJFZ010000000
VERSION     DANJFZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.292"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            100..>395
                     /locus_tag="PWN45_002921"
     CDS             100..>395
                     /locus_tag="PWN45_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0624595.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYS"
BASE COUNT          149 a           49 c           67 g          130 t
ORIGIN      
        1 tgtcgacaca atccttttaa gacgctagta gaatttaaat gacttctaat gtatatgaaa
       61 gtgtatcaat ataaaaccaa ttgaaaagaa gtggagacat tgctttgtga aactgaaaat
      121 attaataaga atcccaaata tagaattatc aaatacaaag atgaatattt gatgattgat
      181 ttagtaagta catggttagc actctttttc ccaatgatta attggctgat tccaaaaaag
      241 tacgtcaaaa tcagcgaaaa agattttgaa actttaaaca ttgtgaagac agctaaaatc
      301 aattcttttt ggccagtggc aggaagtacg gtcttatttg gtgttatgtt aagaaggtat
      361 tcccatttat ttatcgttaa atatgaatat agtat
//
LOCUS       DANJFZ010000284          389 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.293, whole genome shotgun sequence.
ACCESSION   DANJFZ010000284 DANJFZ010000000
VERSION     DANJFZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.293"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>389
                     /locus_tag="PWN45_002922"
     CDS             <1..>389
                     /locus_tag="PWN45_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDL0624596.1"
                     /translation="TKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPNYL
                     KITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALCNL
                     YYFAAVQNHYDLFNYWLMGSISIIISY"
BASE COUNT          131 a           43 c           56 g          159 t
ORIGIN      
        1 actaaagaaa atattgatac tcttcgaaag ccaggcgcac aagccttaag tttaatatca
       61 ttgtttttga tacttttttc atgtttgact ttcttttttg gtttagatta tgaaaggttt
      121 ccaaattatt taaagataac gacaattata gaattaataa ttattgtaat tagtttactt
      181 caatggatta gatttataga tttcgaaaaa gaaagtgcac agaaatataa aaaaatatat
      241 gctcgatttt tagttgttat aaatgtgtta actactatca ctgtagtatt tgcactgtgt
      301 aatctttatt attttgcagc tgtacaaaac cattatgatt tatttaatta ttggttgatg
      361 ggttcgattt caatcataat tagctattt
//
LOCUS       DANJFZ010000285          377 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.294, whole genome shotgun sequence.
ACCESSION   DANJFZ010000285 DANJFZ010000000
VERSION     DANJFZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.294"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            113..>377
                     /locus_tag="PWN45_002923"
     CDS             113..>377
                     /locus_tag="PWN45_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0624597.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMW"
BASE COUNT          133 a           64 c           52 g          128 t
ORIGIN      
        1 acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt ctgtgttggg
       61 tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc ttatgtataa
      121 aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa ttcctcaaat
      181 tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg aattcgataa
      241 attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa aaatcacctt
      301 atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac ttcatgacag
      361 tattcgaatg atgtggt
//
LOCUS       DANJFZ010000286          367 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.295, whole genome shotgun sequence.
ACCESSION   DANJFZ010000286 DANJFZ010000000
VERSION     DANJFZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.295"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..80)
                     /locus_tag="PWN45_002924"
     CDS             complement(<1..80)
                     /locus_tag="PWN45_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0624598.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYL"
     gene            complement(122..>367)
                     /locus_tag="PWN45_002925"
     CDS             complement(122..>367)
                     /locus_tag="PWN45_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL0624599.1"
                     /translation="LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQ
                     RDNNKHLTKLDYTLFIFIIVLAIIGIVRLITGNISVF"
BASE COUNT          141 a           54 c           36 g          136 t
ORIGIN      
        1 tttaagtatg atgctaattg tttaagatta ttatcaaatt catattctga atttttaata
       61 taaatatttt cttcatacat aactataaac cccttaaaaa ttacttagtt taaatcataa
      121 cttaaaatac agaaatatta cctgtaatca aacgaactat tcctattatt gcaagtacaa
      181 tgatgaatat gaataacgta taatccaatt ttgtcaaatg tttgttatta tccctttgta
      241 cgtagctgta tacgagtaat ccaggtatat ataacaacat cgttaatagt aaataatcta
      301 atccagctgc ataaaccaac caaattgtgt aaatagatgc aataattcct attatccatt
      361 gttttaa
//
LOCUS       DANJFZ010000287          361 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.296, whole genome shotgun sequence.
ACCESSION   DANJFZ010000287 DANJFZ010000000
VERSION     DANJFZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.296"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(24..>361)
                     /locus_tag="PWN45_002926"
     CDS             complement(24..>361)
                     /locus_tag="PWN45_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624600.1"
                     /translation="EKEFEYGRLLVGQHKRDDLDVYYFGDTFLMCTIISFKTFEIKET
                     VELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLMLTDFQKENYNKYIQGQKQR
                     VIFENGHFV"
BASE COUNT          122 a           67 c           32 g          140 t
ORIGIN      
        1 actctaatta tattcactat caattagaca aaatggccat tttcaaatat cacgcgttgt
       61 ttctgacctt gaatatattt attataattc tctttttgaa aatcagttaa cattaattta
      121 gatgtaccgt attttaacac tttttgcatt gtttctattc tcatttttct aaataaccat
      181 ccatctttta acacaatacg attaacagca tcatatgata attctactgt ttctttaatt
      241 tcaaatgtct tgaatgaaat aatcgtgcac attaaaaacg tatcaccaaa gtaataaaca
      301 tctaaatcat cacgtttatg ttgtccaact aacaaacgac catattcgaa ctctttttct
      361 g
//
LOCUS       DANJFZ010000288          353 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.297, whole genome shotgun sequence.
ACCESSION   DANJFZ010000288 DANJFZ010000000
VERSION     DANJFZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.297"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(67..237)
                     /locus_tag="PWN45_002927"
     CDS             complement(67..237)
                     /locus_tag="PWN45_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624601.1"
                     /translation="MPSRWVKVKTFKSLSTSKLEKKLQNFKSYNSFDIIEIKRHSYLF
                     LNIVEVYYKDKT"
BASE COUNT          134 a           34 c           35 g          150 t
ORIGIN      
        1 aattatacgg ctggaaattc aatgctgtaa aaatagacag attttaaaaa agtagtaata
       61 aaaagtctaa gttttatctt tgtaataaac ttcgactata tttaaaaata aatatgaatg
      121 tcttttaatt tctataatat caaagctatt atatgatttg aaattttgta actttttctc
      181 taatttggaa gtagataatg acttaaaggt tttaactttt acccaacgac ttggcatttt
      241 atcactccta tgtaatgtat tacttcaata tacccttaaa tttttataat taattatatt
      301 gtaatattat tttattttat aaaaatagtt taataaaatg tttaattttt ata
//
LOCUS       DANJFZ010000289          343 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.298, whole genome shotgun sequence.
ACCESSION   DANJFZ010000289 DANJFZ010000000
VERSION     DANJFZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.298"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..>343
                     /locus_tag="PWN45_002928"
     CDS             <1..>343
                     /locus_tag="PWN45_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0624602.1"
                     /translation="KVYYSEVYKYSDNLIKVFSEDVWNIMGYKRYNEYFTNRDDTTLK
                     ITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIFYKIQPDNIYITN
                     QYGKEAVSRLK"
BASE COUNT          144 a           34 c           59 g          106 t
ORIGIN      
        1 ataaagtata ttactctgaa gtttataaat atagtgataa tttgataaaa gtattctctg
       61 aagacgtttg gaacataatg gggtataaaa gatataatga atattttaca aacagagatg
      121 atactacatt aaaaattact aatttatata ttgggcaatc ttttggtaca gagggtaata
      181 gaaatgtcat tgatagaata gggaaaggtc atgaaaaatt acaagaaatt atgacttatc
      241 agccagaaaa tagaaagact gtcttaatat tttacaagat acagcctgac aatatttata
      301 ttacaaacca atatggtaaa gaggctgtgt ctcgattaaa aag
//
LOCUS       DANJFZ010000290          343 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.denovo.299, whole genome shotgun sequence.
ACCESSION   DANJFZ010000290 DANJFZ010000000
VERSION     DANJFZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.denovo.299"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
BASE COUNT          137 a           58 c           25 g          123 t
ORIGIN      
        1 gagaaagtaa tattttattc aaagaatatg acttcatatt aaatcaaacc tcacattctt
       61 attagattgc aaattcaata gtcctaccag aattgaaatc aaaacattca aactatttta
      121 tcgttttaac ttcctcgacc attttcacct ccttataatc tatataaatt atgtttacca
      181 cacgattctt tacactcgaa aaattaattt tgttaagaaa catttagcca tgtaactttg
      241 taataaaaac attttcaaat aaatgtacca aaaactttca tgaaataaag tatacttttc
      301 aaatctatta attttagaat cataaaacca aatgctatat tac
//
LOCUS       DANJFZ010000291         6013 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.136, whole genome shotgun sequence.
ACCESSION   DANJFZ010000291 DANJFZ010000000
VERSION     DANJFZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.136"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(90..794)
                     /locus_tag="PWN45_002929"
     CDS             complement(90..794)
                     /locus_tag="PWN45_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDL0624603.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1039..1233
                     /locus_tag="PWN45_002930"
     CDS             1039..1233
                     /locus_tag="PWN45_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDL0624604.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1245..1496
                     /locus_tag="PWN45_002931"
     CDS             1245..1496
                     /locus_tag="PWN45_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDL0624605.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1534..2658
                     /locus_tag="PWN45_002932"
     CDS             1534..2658
                     /locus_tag="PWN45_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDL0624606.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2674..3111
                     /locus_tag="PWN45_002933"
     CDS             2674..3111
                     /locus_tag="PWN45_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDL0624607.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            3535..4491
                     /locus_tag="PWN45_002934"
     CDS             3535..4491
                     /locus_tag="PWN45_002934"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL0624608.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            4691..5170
                     /locus_tag="PWN45_002935"
     CDS             4691..5170
                     /locus_tag="PWN45_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0624609.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5185..>6013
                     /gene="fakB2"
                     /locus_tag="PWN45_002936"
     CDS             5185..>6013
                     /gene="fakB2"
                     /locus_tag="PWN45_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDL0624610.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     "
BASE COUNT         2216 a          900 c         1048 g         1849 t
ORIGIN      
        1 aagttggggt gggacgacga aataaatttt gcgaaaatat tatttctgtc ccactccctt
       61 aaaacttatt cttttgtgta gtaagtgcgt taatagcctt gatctaactt atcaatctta
      121 cctttacgat aaaatgattt agcaatatat ccaaatggta cattgaaaac tgttgaagct
      181 aattttaata cgtacgttgt aataaatatt tcaaatacaa ctgtaccagg taaacttccg
      241 ataaatgcga tagctacaaa taaagctgta tcaattattg agcttaaaaa tgtactacca
      301 tatgcacgga tgaaaaacgt tttatcagaa ctaaatactt ttttaattag tgaaaagata
      361 aatacatcaa tatgttgacc aataatatat gcgacgattg agcctaaagc aatgcgtggc
      421 acaacatcaa agattgcgtg taatgctttt tgtgccatat cttctggtgc aggaataaaa
      481 tgtaatgaca attgcataac aataatcata attaatgttg atgaaaagcc taaccaaact
      541 gctctttttg caactctacg cccataaata tcgtttaata tatctgttgc taaataaata
      601 gaagcaaaca tgacattacc taaagttgct gaaataccaa agatttctac agttttaatc
      661 acttgtatgt tggcaatgat tgtgccaatt gcaacccatg caattaaacc ttgtttacca
      721 aaaaagcgat acataagtac cataagcacg aacgttgcaa taaacgtaac tagtcctaaa
      781 atttcattat acatattaaa atgtcctcct aaattttgat catgcgggtg tttagaaacc
      841 gctcaataaa taactaactc attttactga aaaatcgttt gcgatgcaat agcatttgaa
      901 tataaataca ttttacgtat agaattgtaa aaggtttcat tcaaatctta gggtcaaaaa
      961 tgttataata tttttatgtc aaatttaaaa cagtaacact tatttacaag gttgcaatat
     1021 tttgaagtaa taaaggaagt gtcgcgtatt ttaacttttt cagagcaaaa tgcactcgcg
     1081 aaaatagatg atttaatgaa tacttattgc aatcaatgtc caatcaaaac tcgtctgcgt
     1141 aaattagagg ggaaaacgaa ggcgcatcat ttttgtatca atgagtgttc aatagggaaa
     1201 gaaataaaac aattaggaaa tgaacttcaa taggaggaag tcaaatgaaa attatatcta
     1261 tatcagaaac accgaaccac aacacaatga agattacact tagtgaaagc agagaaggta
     1321 tgacatcaga tacgtatact aaagttgatg attcacagcc agcatttatt aatgacatct
     1381 taaaggttga aggcgttaaa tcaattttcc atgttatgga ctttatttca gtagataaag
     1441 aaaatgacgc aaattgggaa acagtattgc caaaagtaga ggctgtattc gaataaattt
     1501 ttcatcaact agtattcggg gggaataaag tatatggaaa ttttacgtat agagccaaca
     1561 ccaagtccaa atacaatgaa agttgttttg tcatatacaa gagaagacaa gttatctaat
     1621 acttataaaa aagtagaaga aacacaacca agatttataa atcagttgtt atctatagat
     1681 ggtatcactt ccatttttca tgtcatgaac ttcttagctg ttgataaggc accaaaagct
     1741 gattgggaag tcatattacc tgatattaaa gctgcttttt ctgatgcgaa taaggtttta
     1801 gaatctgtaa atgaacctca aattgacaat cattttggtg aaattaaagc tgaattatta
     1861 acttttaagg gtataccgta tcaaattaag ctaacttctg ctgaccaaga attaagagaa
     1921 caattaccac aaacatatgt tgaccatatg actcaagcgc aaacagcaca tgacaatatt
     1981 gtttttatgc gtaaatggct agatttagga aatcgctatg gaaatattca agaagtaatg
     2041 gatggtgtcc tagaagaagt gctaactacc tatccagaat cacagttacc cgtattggta
     2101 aaacatgctt tagaagaaaa tcacgcaact aataattatc atttctatcg acatgtctct
     2161 ttggatgaat atcatgcaac tgataattgg aagactcgat tacgaatgtt aaaccatttt
     2221 ccaaagccga cttttgaaga tataccgctg cttgatttag ctttatctga tgaaaaagta
     2281 ccggttagac gtcaagcgat tgtattatta ggtatgattg aaagtaaaga aattttaccg
     2341 tatttatata aggggcttcg tgataaaagt cctgttgtaa gaagaacagc aggggattgc
     2401 ataagcgatt tagggtatcc agaggcacta ccagaaatgg tgctactatt agatgatcca
     2461 cagaaaatcg ttaggtggcg tgctgctatg tttatctttg atgaaggtaa tgcagagcag
     2521 cttcccgcac taaaagccca tattaatgac aatgcgtttg aagttaaatt acaaattgaa
     2581 atggccatat ctcgtattga aaacggagat gaagcacttg gttcagtatg gaaacaaatg
     2641 gcaaacagaa caatttaatt ggaggaatta aatatgaatg catatgatgc ttatatgaaa
     2701 gaaattgcgc aacaaatgcg tggcgaatta actcaaaatg gttttacaag tttagaaacg
     2761 agcgaagcag tatcggagta tatgaaccaa gtaaatgctg atgacactac ttttgtagtt
     2821 attaactcta catgcggctg tgcagctgga ttagcaagac cagctgcagt agcagttgca
     2881 acacaaaatg aacatagacc tacaaataca gttacagttt ttgctgggca agataaagaa
     2941 gcaactgcta caatgcgaga attcattcag caagcaccat ctagtccttc gtatgcttta
     3001 ttcaaaggtc aagatttagt ttattttatg cctagagaat ttatcgaagg tagagatatt
     3061 aatgacattg caatggactt aaaggatgcc tttgacgaaa attgtaaata gtacacataa
     3121 ataaatataa aggttaacac attttataat attaaaaatg gtgtctgtca ttgaaaatag
     3181 agaatatagt tgtattctat ttgttaaata aagtccgttt ttaccaacta tattttctag
     3241 aaatttaact gttttaatag gacatcaaac ataatattca aatcatgtgt taacctcttt
     3301 tttaaaattt tttagcatta aagttataga tttgggtaaa caattaccaa ttggaaacat
     3361 atatcacgtt acgatggggt aggtacttaa tcagcatttt ataaataaag taacggaatt
     3421 catgatatta atatcatatt cctaaaatga gtgataacaa aatgctacat aaagttaagt
     3481 tatatcaaac taaatataca tactataaat aatgaaaatg aggtgttatc gcatatgttg
     3541 aattcatttg atgcagcata tcacagtctt tgtgaagaag ttttagaaat aggaaataca
     3601 cgaaatgatc gcacaaatac aggtacgatt tcgaaatttg gtcatcaact tcgctttgac
     3661 ttatctaaag gatttccact attaacgaca aagaaagttt cttttaaatt agtagcaacc
     3721 gaattattat ggttcattaa aggagataca aacatccaat acttattaaa atataataat
     3781 aatatatgga acgaatgggc ttttgaaaat tatatcaaat cagacgagta taaaggtcca
     3841 gatatgacag atttcgggca tcgtgcattg agtgatcctg aatttaacga acaatataaa
     3901 gaacaaatga aacaatttaa gcaacgtatt cttgaagatg atacatttgc gaagcaattc
     3961 ggggatttag gaaatgttta tggtaaacaa tggcgagatt gggttgataa agatggtaat
     4021 cattttgatc aacttaaaac agtaattgaa caaattaagc ataatccaga ttcaaggcga
     4081 cacatcgtat ctgcatggaa tccaacagaa attgatacaa tggcacttcc gccttgtcat
     4141 accatgttcc agttttatgt ccaagatggt aagttaagtt gccagttata ccaacgtagc
     4201 gcagatatct ttttaggtgt gccatttaat atcgcaagct acgctttatt gacacacctt
     4261 attgccaaag aatgtggact tgaagtgggt gaatttgtgc atacatttgg agatgcacat
     4321 atttattcaa atcatattga tgcgattcaa acacaattag cacgtgaaag cttcaatcct
     4381 ccaacattaa aaattaacag tgacaagtct attttcgaca taaattatga agatttggaa
     4441 attgttgact atgaatcaca tccagcaata aaagctccaa tagcagtgta gtcattgcat
     4501 agttagctaa ccatatagac atcaaaatga catcatagta ttttcaagtg caaaaaagta
     4561 cttttttgtg ttaaacgttt tcataaatta tgcaaaatca ttatttctat cacactttat
     4621 gataaaaatt gtgttaaatt aaagataact tagtaataaa aaatgaaatg atagaagaag
     4681 gaggataatt atgactttat ccattctagt tgcacatgac ttgcaacgag taattggttt
     4741 tgaaaatcaa ttaccttggc acctaccaaa tgatttgaag catgttaaaa aattatcaac
     4801 aggtcatact ttagtaatgg gtcgtaagac atttgaatcg attggtaaac cactaccgaa
     4861 tcgtcgaaat gttgtactta cttcagatac aagtttcaac gtagagggcg ttgatgtaat
     4921 tcactctatt gaagatattt accaactacc gggccatgtt ttcatatttg gagggcaaac
     4981 attatttgaa gaaatgattg ataaagtgga cgacatgtat attactgtta ttgaaggtaa
     5041 attccgtggt gatacgttct ttccacctta tacatttgaa gactgggaag ttgcctcttc
     5101 agttgaaggt aaactagatg agaaaaatac aattccacat acctttctac atttaattcg
     5161 taaaaaataa gggggaaaac gaccatgaca aaacagatta tagtaacaga ctcaacatcc
     5221 gatttatcta aagaatactt agaagcaaac aacattcatg taattccttt aagtttaact
     5281 attgaaggag cttcatacgt tgaccaagta gatattacat cagaagaatt tattaatcat
     5341 attgaaaatg atgaagatgt aaagacaagt cagccagcca taggtgaatt tatatctgct
     5401 tatgaagaac taggaaaaga tggctctgaa atcataagta ttcatctttc ttcaggatta
     5461 agtggtacat ataacactgc ttaccaagca agtcaaatgg tagatgctaa tgtaactgtt
     5521 attgattcaa aatctatttc ttttggttta gggtatcaaa tacaacacct agtagagctt
     5581 gtaaaagaag gtgtctcaac ttctgaaata gttaaaaagt taaatcattt aagagaaaac
     5641 attaaattat ttgtagttat agggcaattg aatcaattaa ttaaaggtgg cagaattagt
     5701 aaaacaaaag gtttgattgg taatcttatg aaaattaaac caattggtac actagatgat
     5761 ggtcgcttag agcttgtgca caatgcgaga actcaaaatt ctagtatcca atacttgaaa
     5821 aaggaaattg ctgaatttat aggagatcat gaaatcaaat ccattggtgt cgcacatgct
     5881 aacgtcattg aatatgttga taaattgaag aaagttttta atgaagcttt tcatgtgaat
     5941 aattacgata taaatgtaac tacaccagtt atttctgcac atactggtca aggtgcgatt
     6001 ggcctcgtag tcc
//
LOCUS       DANJFZ010000292         4002 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.169, whole genome shotgun sequence.
ACCESSION   DANJFZ010000292 DANJFZ010000000
VERSION     DANJFZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.169"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            264..1730
                     /gene="msr(A)"
                     /locus_tag="PWN45_002937"
     CDS             264..1730
                     /gene="msr(A)"
                     /locus_tag="PWN45_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDL0624611.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            1829..2728
                     /locus_tag="PWN45_002938"
     CDS             1829..2728
                     /locus_tag="PWN45_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL0624612.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3214..3822
                     /locus_tag="PWN45_002939"
     CDS             3214..3822
                     /locus_tag="PWN45_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0624613.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
BASE COUNT         1595 a          579 c          693 g         1135 t
ORIGIN      
        1 attaaaatga cagcttctat gagactcaaa taaacctttt tgaatacaaa actaaattaa
       61 ttcaaaaagg cttgtccgca atacacactc ataggatgac ttatgtcatt ttttatcctt
      121 cgataggaat aataaacgct ttcaaatact aaaacatcat aataaatttt taagtgtttg
      181 aaagtgtaat gtgaatgaga cgggagtagc ctgtctcatt tttttgcgta cacgtccttt
      241 aaatatacaa ggagtgtata aatatggaac aatatacaat taaatttaac caaatcaatc
      301 ataaattgac agatttacga tcacttaaca tcggtcatct ttacgcttac caatttgaaa
      361 aaatagcact tattgggggt aatggtactg gcaaaaccac attactaaat atgattgctc
      421 aaaaaacaaa accagaatct ggaacagttg aaacggttgg cgaaattcaa tattttgaac
      481 agcttaacat ggatgtggaa aatgatttta acacgttaga cggtagttta atgagtgaac
      541 tccatatacc tatgcataca accgacagta tgagtggtgg tgaaaaagca aaatataaat
      601 tagctaatgt catatcaaat tatagtccga tattactttt agatgaacct acaaatcact
      661 tggataaaat tggtaaagat tatctgaaaa atattttaaa atattactat ggtactttaa
      721 ttatagtaag tcatgataga gcacttatag accaaattgc tgacacaatt tgggatatac
      781 aagaagatgg cacaataaga gtgtttaaag gtaattacac acagtatcaa aatcaatatg
      841 aacaagaaca gttagaacaa caacgtcaat atgaacagta tataagtgaa aaacaaagat
      901 tgtcccaagc cagtaaagct aaacgaaatc aagcgcaaca aatggcacaa gcatcatcaa
      961 aacaaaaaaa caaaagtata gcaccagatc gtttaagtgc atcaaaacaa aaaggcacgg
     1021 ttgagaaggc tgctcaaaaa caagctaagc atattgaaaa aagaatggaa catttggaag
     1081 aagttgaaaa accacaaagt tatcatgaat tcaatttccc acaaaataaa atttatgata
     1141 tccataataa ttatccaatc attgcacaaa atctaacatt ggttaaagga agtcaaaaac
     1201 tgctaacaca agtacgattc caaataccat atggcaaaaa tatagcgctc gtaggtgcaa
     1261 atggtgtagg taagacaact ttacttgaag ctatttacca ccaaatagag ggaattgatt
     1321 gttctcctaa agtgcaaatg gcatactatc gtcaacttgc ttatgaagac atgcgtgacg
     1381 tttcattatt gcaatattta atggatgaaa cggattcatc agaatcattc agtagagcta
     1441 ttttaaataa cttgggttta aatgaagcac ttgatcgttc ttgtaatgtt ttgagtggtg
     1501 gggaaagaac gaaattatcg ttagcagtat tattttcaac gaaagcgaat atgttaattt
     1561 tggatgaacc aactaatttt ttagatatta aaacattaga agcattagaa atgtttatga
     1621 ataaatatcc tggaatcatt ttgtttacat cacatgatac aaggtttgtt aaacatgtat
     1681 cagataaaaa atgggaatta acaggacaat ctcttcatga tataacttaa aaactataca
     1741 ctaatttata ttgaatatta ggttcattta tatcaatata aaaaatacgt tcacaagaaa
     1801 aatataatag aaataaggat ccaataaaat gactcgacat aatgaaatta ttaaatgtgc
     1861 agaaaaatat caattacaca tccaacctca aacaatctca ttgaatgaat cgggacttga
     1921 ttttcaagtt gcatttggaa aagataaaca tggagtagaa tgggttttga gactaccaag
     1981 aagacctgac gtttataaac gaacaaaacc cgaaaaacaa acggtagact tcttacagaa
     2041 gaatgtttca tttgaaatac cgaagtggaa agtacatgca aaagacctta ttgcttaccc
     2101 aaaacttaca ggtaaacccg cagccacaat agatccagaa atacaaaatt atgtatggga
     2161 aattgaacac aaaccattac cagaaaactt tattaacaca ttagctgaaa cactcgtaga
     2221 tttacacaac ataccagaag aaaacattaa cgttcagcat ataaatatca aaaccataca
     2281 agaaataaaa aatgactttc aaagaagaat gaataaagtt aaagaaactt atggcgtatc
     2341 agatgaatta tggaacagat ggaaacaatg gttagaaaac gacgaactat ggcctcgaca
     2401 tgcgaccatg atacatgggg acttacatcc aggacatata atggtagata accaagcaaa
     2461 cgtcacaggt ctcatagact ggactgaagc aacccactcc gacccatcaa tggactttat
     2521 aggacaccat cgtgtattcg acgacgaagg attagagcaa cttataacag catacggtaa
     2581 agctggaggt gaaatatggc cacgaatgaa agagcatata atagaactca atgcagtatt
     2641 cccaatgttt atcgctgagt ttgctatgga atcaggagaa tcggcgtatg aaacgatggc
     2701 attgaaagag ttaggtatga aagagtagaa cttatattaa aagaggccca ttttaaagac
     2761 gaaggcaatc ctgatgaaat tttaaaactg cgtttaatta gtgatggtct ttggttttct
     2821 ataatgtatt aaatatctgg acatggaaca aatgcaacaa atggaaagaa tcgttttcga
     2881 aatttctaac tccttaaatc aggaggataa ataaacggtc attttcattg aaagcactta
     2941 tataaatttc atcaatttta atacctaccg taaatacatt tcataagtat taaacagatg
     3001 attaaaattt ttagttaatt taaatattta tgatagagat gaccttcaaa tgaacggttc
     3061 tgttacaaag aaaaaatcta tatttatttt tagtaagttt taattataga tatgtacact
     3121 ttgagtacac cctaaccata caaaaagaat tgttgaaatt ataacaatta aaacgtatga
     3181 ttagggtgta tatttattat ggagtgaaga tgaatgatta taggttatgc gagagtatca
     3241 tcgatagatc aaaatttaga aagacaattg gataatttaa agacgtttgg tgtggagaaa
     3301 atctttacag agaaacagtc gggaaagtca gtggaaaata gacctgtatt tcaagaagca
     3361 ctcaattttg tcagaatggg agatcgattc gttgtggaat ctattgatcg tttaggacgt
     3421 aactatgatg aaattattga aactgtgaat tatttaaaag aaaaagacgt tcagttaatg
     3481 attaccagct tacctatgat gaatgaagta attggcaatc cattactgga taagtttatg
     3541 aaagatttaa tcattcaaat attagcaatg gtttcagaac aagagagaaa tgaaagtaaa
     3601 cgtcgacaag cgcaagggat tcaagttgcg aaggaaaaag gcgtatataa agggcgccct
     3661 ttactttatt caccaaatgc taaagaccca cagaaacgtg ttatttatca tcgcgtggtg
     3721 gaaatgttag aagaaggcaa agcgattagt aagattgcga aagaagttaa tatcacaaga
     3781 caaacagttt atagaattaa acatgataag ggattatctt gataaattga ttgtcaatgc
     3841 aaatgagcgt ctctcacagt gtctcaaatt ccataacata ttattgaatt gagattttaa
     3901 taattataca agaaaaaaga gtacgagttc gcagctttct atatagtaat tgttttacta
     3961 ataaggtgtt ttaaaatata ttataatgta aaaagtattt ga
//
LOCUS       DANJFZ010000293         3711 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.177, whole genome shotgun sequence.
ACCESSION   DANJFZ010000293 DANJFZ010000000
VERSION     DANJFZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.177"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            <1..1958
                     /gene="mecA"
                     /locus_tag="PWN45_002940"
     CDS             <1..1958
                     /gene="mecA"
                     /locus_tag="PWN45_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDL0624614.1"
                     /translation="FGIYFYASKDKEINNTIDAIEDKNFKQVYKDSSYISKSDNGEVE
                     MTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTNYGNIDRNVQFNFVKE
                     DGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNNVELANTGTAYEIGIVPKN
                     VSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMDEYLSDFAKKFHLTT
                     NETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGKKGLEKLYDKKLQH
                     EDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNMKNDYGSGTAIH
                     PQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITTSPGSTQKILT
                     AMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQAIESSDNIFF
                     ARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNEILLADSGYGQGEIL
                     INPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISKENINLLTDGMQQVVNKTH
                     KEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMAINVKDVQDKGM
                     ASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2004..2432)
                     /locus_tag="PWN45_002941"
     CDS             complement(2004..2432)
                     /locus_tag="PWN45_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDL0624615.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2529..3272)
                     /locus_tag="PWN45_002942"
     CDS             complement(2529..3272)
                     /locus_tag="PWN45_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDL0624616.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1327 a          599 c          562 g         1223 t
ORIGIN      
        1 ggtttggtat atatttttat gcttcaaaag ataaagaaat taataatact attgatgcaa
       61 ttgaagataa aaatttcaaa caagtttata aagatagcag ttatatttct aaaagcgata
      121 atggtgaagt agaaatgact gaacgtccga taaaaatata taatagttta ggcgttaaag
      181 atataaacat tcaggatcgt aaaataaaaa aagtatctaa aaataaaaaa cgagtagatg
      241 ctcaatataa aattaaaaca aactacggta acattgatcg caacgttcaa tttaattttg
      301 ttaaagaaga tggtatgtgg aagttagatt gggatcatag cgtcattatt ccaggaatgc
      361 agaaagacca aagcatacat attgaaaatt taaaatcaga acgtggtaaa attttagacc
      421 gaaacaatgt ggaattggcc aatacaggaa cagcatatga gataggcatc gttccaaaga
      481 atgtatctaa aaaagattat aaagcaatcg ctaaagaact aagtatttct gaagactata
      541 tcaaacaaca aatggatcaa aattgggtac aagatgatac cttcgttcca cttaaaaccg
      601 ttaaaaaaat ggatgaatat ttaagtgatt tcgcaaaaaa atttcatctt acaactaatg
      661 aaacagaaag tcgtaactat cctctaggaa aagcgacttc acatctatta ggttatgttg
      721 gtcccattaa ctctgaagaa ttaaaacaaa aagaatataa aggctataaa gatgatgcag
      781 ttattggtaa aaagggactc gaaaaacttt acgataaaaa gctccaacat gaagatggct
      841 atcgtgtcac aatcgttgac gataatagca atacaatcgc acatacatta atagagaaaa
      901 agaaaaaaga tggcaaagat attcaactaa ctattgatgc taaagttcaa aagagtattt
      961 ataacaacat gaaaaatgat tatggctcag gtactgctat ccaccctcaa acaggtgaat
     1021 tattagcact tgtaagcaca ccttcatatg acgtctatcc atttatgtat ggcatgagta
     1081 acgaagaata taataaatta accgaagata aaaaagaacc tctgctcaac aagttccaga
     1141 ttacaacttc accaggttca actcaaaaaa tattaacagc aatgattggg ttaaataaca
     1201 aaacattaga cgataaaaca agttataaaa tcgatggtaa aggttggcaa aaagataaat
     1261 cttggggtgg ttacaacgtt acaagatatg aagtggtaaa tggtaatatc gacttaaaac
     1321 aagcaataga atcatcagat aacattttct ttgctagagt agcactcgaa ttaggcagta
     1381 agaaatttga aaaaggcatg aaaaaactag gtgttggtga agatatacca agtgattatc
     1441 cattttataa tgctcaaatt tcaaacaaaa atttagataa tgaaatatta ttagctgatt
     1501 caggttacgg acaaggtgaa atactgatta acccagtaca gatcctttca atctatagcg
     1561 cattagaaaa taatggcaat attaacgcac ctcacttatt aaaagacacg aaaaacaaag
     1621 tttggaagaa aaatattatt tccaaagaaa atatcaatct attaactgat ggtatgcaac
     1681 aagtcgtaaa taaaacacat aaagaagata tttatagatc ttatgcaaac ttaattggca
     1741 aatccggtac tgcagaactc aaaatgaaac aaggagaaac tggcagacaa attgggtggt
     1801 ttatatcata tgataaagat aatccaaaca tgatgatggc tattaatgtt aaagatgtac
     1861 aagataaagg aatggctagc tacaatgcca aaatctcagg taaagtgtat gatgagctat
     1921 atgagaacgg taataaaaaa tacgatatag atgaataaca aaacagtgaa gcaatccgta
     1981 acgatggttg cttcactgtt ttattatgaa ttattaataa gtgctgttac ttctccctta
     2041 aatacaattt cttcattttc attgtatgtt gaaagtgaca ctgtaacgag tccattttct
     2101 ttttttatgg atttcttatt tgtaatttca gcgataacgt acaatgtatt acctgggtat
     2161 acaggtttaa taaatttaac gttattcatt tgtgttcctg ctacaacttc ttctccgtat
     2221 ttaccttctt ctacccataa tttaaatgat attgaaagtg tatgcatgcc agatgcaatg
     2281 atacctttaa atctactttg ttctgctttt tctttatcta tatgcatata ttgaggatca
     2341 aaagttgttg caaattggat aatttcttct tctgtaatat gaaggctttt tgttttgaat
     2401 gtttctccta ctataaaatc atcgtatttc atatatgtct ctctttctta ttcaaattaa
     2461 ttttttagta tgtaacatgt taaaggtaag tctaccgtca ctgaaacgta agactcacct
     2521 ctaactttct attgagacaa atgcaccatt ttatctgcat tgtctgtaaa gataccatca
     2581 actccccaat tagcaagttg gtttgcacgt gctggtttgt ttacagtcca tacgttcaat
     2641 tcataacccg cttcttttac catttttact tttgctttag taagtttggc atcttcagtg
     2701 tttactattt tagcattaca gtaatctaaa agtgttctcc agtcttcacg aaacgaagtt
     2761 gtatggaata taactgctct gttatattgt ggcatgattt cttctgcaag tttaacaagc
     2821 acaacattaa agcttgaaat gagcacttct tgattctgat ttaagtttgt taattgttct
     2881 tccacttgct taaccatact tttagaaagt gctagtccat tcggtccagt aatacctttt
     2941 aattctacat ttaaattcat attatattca tttgctattt ttactacatc atcgaaagtt
     3001 ggcaaatgtt catctttgaa tttttcacca aaccaagatc ctgcagaagc atctttaatt
     3061 tcatcataat tcaattcagt tatttccccg gacatatttg tagtccgttc taaataatca
     3121 tcatgaatga taatcagttg ttcatctttt gtaattgcaa catctaactc caaccagttt
     3181 ataccttcta cttctgaagc agctttaaat gatgcaattg tattttccgg agctttacta
     3241 ggtaatcctc tatgtccata tacagttagc atattacctc tccttgcatt tttatttttt
     3301 taattaacgt aactgtatta tcacattaat cgcactttta tttccattaa aaagagatga
     3361 atatcataaa taaagaagtc gatagattcg tattgattat ggagttaatc tacgtctcat
     3421 ctcattttta aaaaatcatt tatgtcccaa gctccatttt gtaatcaagt ctagtttttc
     3481 tgtacccctt atctgcaatt ttacttagga ttgcttttaa cttacccctt atcagcaatt
     3541 ttactgagaa ctgcttttaa cgcacctctt atctgcaatt ttgcctagaa ctgcttttaa
     3601 cgtacctctt atctgcaatt ttactgagaa ctgcttttaa cttacccctt atcagcaatt
     3661 ttgcatggaa ttgcttttaa cgtacctctt atctgcaatt ttacttagaa c
//
LOCUS       DANJFZ010000294         3578 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.179, whole genome shotgun sequence.
ACCESSION   DANJFZ010000294 DANJFZ010000000
VERSION     DANJFZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.179"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(389..1234)
                     /gene="blaZ"
                     /locus_tag="PWN45_002943"
     CDS             complement(389..1234)
                     /gene="blaZ"
                     /locus_tag="PWN45_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDL0624617.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            1341..3098
                     /gene="blaR1"
                     /locus_tag="PWN45_002944"
     CDS             1341..3098
                     /gene="blaR1"
                     /locus_tag="PWN45_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDL0624618.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3088..3468
                     /gene="blaI"
                     /locus_tag="PWN45_002945"
     CDS             3088..3468
                     /gene="blaI"
                     /locus_tag="PWN45_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDL0624619.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT         1378 a          440 c          474 g         1286 t
ORIGIN      
        1 cctctataat aaacatattg taatataatt tatgtattat ttaggtttac cctaatcgaa
       61 caataaaaaa tacttgtaaa atgctattgt cactttgctt gatatatgag aattatttaa
      121 ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat
      181 gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc
      241 tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt
      301 ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt
      361 tatcagtatt tattatgcat ttagaatatt aaaattcctt cattacactc ttggcggttt
      421 cacttatcaa cttatcattt ggcttatcac ttttattgtc tttattcgta aaaatgacta
      481 aaacaatagg ttcagattgg cccttaggat aaacaaaagc aacatcattt ctagaagcat
      541 atgttattgc ttgaccactt ttatcagcaa ccttatagtc ttttggaaca ccatctttaa
      601 ttaaagtgtc tccgttttta ttattaaaca ttaaatcaag taagaaattt ttattttttt
      661 tgcttaattt tccatttgcg ataagtttat ttaaagtctt gccgaaagca gcaggcgttg
      721 aagtatcttt tttgctcttt ggtgaatagt aatttaattc tatctcatat ctaactggat
      781 ttgttacttt atctcctagt tcttttagac gttgtttaac ttttttgatt ccaccgattt
      841 cttttataat tttattgttt gctgtattat cactatatgt cattgaagcc tcaataagtt
      901 cttttaaagt gatatctttt cctacatatt tttctaaaat aggagaataa gcaactatat
      961 catctttgtt aatatgtact tttttattta acttattata aggtacttgt tctaacaaaa
     1021 tagcactatt tatcgctttt gaagtcgaag cataggcaaa tctcttatct gaattaaatt
     1081 ttacttcctt accactttta gtatttaaag cataaacacc aatatgagca ttatattttt
     1141 tttctaaatc atttaactct ttggcatgtg aactgtttga attacatgca cttaaaacta
     1201 aagcaattac aattaaaaat attaactttt tcaaaataaa ccctccgata ttacagttgt
     1261 aatttttata aatcaataat attacaattg taatattgat gtcaattgtt tattataaat
     1321 atttgaaaga aggtgtcaaa atggctaaat tactaataat gagcgtagta agcttttgtt
     1381 ttatatttct attgttagta ttttttaggt acatattaaa acgctatttt aattatagtt
     1441 taaattataa agtttggtat ctaactatgc ttgctggatt aattcctttt atcccaatta
     1501 aattctcttt tataaaattt aataatgtga ataatcaagc gcccacagtt gaaagtaagt
     1561 cacacgattt gaaccataac ataaatacca ccaaacctat tcaagagttc acaacagata
     1621 tccataagtt taattgggat tcaattgata atatctgcac agttatttgg atagttttag
     1681 ttattatttt aagttttaaa tttttgaaag ccttattata tcttaaatat ttaaagaaac
     1741 agtcacttta tctaaacgaa aatgaaaaaa ataaaataga tacgatactt ttcaaccatc
     1801 aatataaaaa aaatattgtg attcgaaaag cagaggctat tcaatctcca ataacttttt
     1861 ggtatgggaa atatattatt ttgattccta gttcatattt taaaagtgta attgataaaa
     1921 gactaaaata tataatactg catgaatatg ctcatgctaa aaatagagat actttacatt
     1981 taattatctt taatatcttc tctattatta tgagttacaa tccattagta catattgtaa
     2041 aaagaaagat aattcatgac aatgaagtag aagccgatag atttgtcctt aataatatta
     2101 acaaaaatga atttaaaact tatgcggaat ctattatgga ctccgtatta aaaaccccat
     2161 tttccaacaa aaatatatta agccattcat ttaatggtaa aaaatcatta ctcaaaagta
     2221 gattaattaa tataaaagaa gccgatctga aaaagcagtc aaaattaatt ctaattttta
     2281 tttgtatttt tacttttttt atcatgataa ttcaaagcca gtttttaatg gggcaatcct
     2341 taactgatta taattataaa aagcccttac aaagcgatta ccaaatttta gatgaaagta
     2401 aaaattttgg ttcaaatagt ggatctttcg ttatgtacag catgaagaaa gacaaatact
     2461 atatttataa tgaaaaagaa agtagaaaaa gatattctcc tgattcaact tacaaaattt
     2521 atttagcact gtttggactt gaccgtcata ttataagtga taaaaattca cgtatgtcat
     2581 ggaatcataa acattatctt ttcgagtctt ggaataagga acaagattta aatacagcaa
     2641 tgcaaaattc agttaattgg tacttcgaac gtattagtaa tcaaatacca aagaactata
     2701 ctgcggctca actcaagcaa ttaaattatg gtaatgaaaa tttgggaagt tataaaagct
     2761 attggatgga agatagtttg aaaatatcta atcttgaaca agtaatagtt tttaaaaata
     2821 tgatggaaca aaataaccat tttagtaaaa aagcaaagaa tcaattatct tcttcattat
     2881 tgattaagaa aaatgaaaag tatgaactgt atgggaaaac aggtacaggt atagtaaacg
     2941 ggaagtataa taatgggtgg tttgtaggtt acgtaattac aaatcatgat aagtattatt
     3001 ttgctacaca tttatcagat ggaaacccgt ctgggaaaaa tgctgaatta ataagtgaaa
     3061 aaatattaaa aggaatgggt gttttaaatg accaataagc aagttgaaat atctatggct
     3121 gaatgggatg ttatgaatat aatatgggat aaaaaatcag tatcagctaa tgaaattgta
     3181 gttgaaattc aaaaatataa agaagttagt gataaaacga ttagaacatt aatcacaaga
     3241 ctatataaaa aagagattat aaaacgatac aaatcagaaa atatttattt ttactcatca
     3301 aatattaaag aagacgatat taaaatgaaa actgctaaaa cctttcttaa taaactgtat
     3361 ggaggggata tgaaaagttt agtgctgaat tttgcgaaaa atgaagaatt aaataacaaa
     3421 gaaattgaag aattgcgaga cattttaaat gatattagta aaaagtaatt agaggtatgt
     3481 ttcaaacata atacatatgt aaaccatact tgactcttat taaattagtt ttgttaatga
     3541 catttaaaat atattattaa agatgagagt gtccagaa
//
LOCUS       DANJFZ010000295         3302 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.184, whole genome shotgun sequence.
ACCESSION   DANJFZ010000295 DANJFZ010000000
VERSION     DANJFZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.184"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            complement(<1..1632)
                     /gene="hutU"
                     /locus_tag="PWN45_002946"
     CDS             complement(<1..1632)
                     /gene="hutU"
                     /locus_tag="PWN45_002946"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDL0624620.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHI"
     gene            1792..2676
                     /locus_tag="PWN45_002947"
     CDS             1792..2676
                     /locus_tag="PWN45_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0624621.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            2798..3217
                     /locus_tag="PWN45_002948"
     CDS             2798..3217
                     /locus_tag="PWN45_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDL0624622.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         1106 a          666 c          475 g         1055 t
ORIGIN      
        1 aatatgaata cctttttctt tagctgtttg aatagcgatg tcatagccag catcaacatg
       61 tcgggcaaca cccatacctg ggtcagtcgt caatacacgt tccaatcttc tttcagcacg
      121 ctctgatcca tctgctacaa caaccatacc cgcatgaagt gaatatccca tgccaacacc
      181 gccaccgtga tggaatgaaa tccatgaacc acctgcagct gtgttaatga gtgcattcaa
      241 tacagcccaa tcaccaaccg cgtcactacc atctttcata ctttctgttt cacggttagg
      301 actagcaact gaaccagcat ctaaatggtc tcgtccaata acaattggtg ctgaaatttc
      361 accgtcacgt acaagacgat ttaaagctaa gcccattttc gctctttctc catagcctaa
      421 ccaagcaata cgtgatggta gtccttgata tgaaattttt tcttcagcta aatcaagcca
      481 tcttaataac ttttcatttt ctgggaaaag tttgcgcatt tcttcatccg cacgctcgat
      541 atcttttgga tcaccactca acgcagcaaa gcggaatggc cctttacctt cacagaataa
      601 tggtctaatg taagctggta caaagcctgg gaagtcaaaa gcatttttca ctccgttatt
      661 gaaggctact tgacgaatat tgttaccata atcaaatgct acagcgccac gtttttggaa
      721 ttcaagcatt aattcaacat gctttgccat tgaagcttgt gacagttcaa catatttttt
      781 cggatctttt tcacgcaata ctttcgcttc ttctacagag tatccttgtg gcacatatcc
      841 atttagcgga tcatgtgcac ttgtttggtc agtaataatg tcaattttaa atcctttttc
      901 tagaatcgct tgatggatgt ctacagcatt tccaactaac ccgattgata atccttctcc
      961 acgttctttc gcctcttctg ctaattttaa tgcttcatct aaatcagctg ttttaacatc
     1021 acagtatttc gtatcaattc gcttatcaac acgtgtttca tcaacatcca cgcaaattgc
     1081 taccccatga ttcatagtaa ttgctaacgg ttgcgcacca cccataccac ctaaacctgc
     1141 tgtcagtgta acagtgcctg ctaaatctcc attaaagtgt tgattaccta gctcggcaaa
     1201 tgtctcataa gtaccttgca caataccttg agaaccaata tatatccaac taccggctgt
     1261 catctgtcca tacatgatta aacctttttt atctaattca ttaaaatgat cccagtttgc
     1321 ccattcaggc actaatactg aatttgaaat taatacacgt ggcgcttctt catgtgtttt
     1381 aaatacagca actggctttc ctgattgtac taacattgtc tcatctgatt ctaattctcg
     1441 taacgttttc tctattgctt caaaagcttc ccaattacgt gctgcttttc caataccacc
     1501 ataaacaact aaatcttctg gtctttcagc aacttctggg tctaaattgt tgtataacat
     1561 tctaagtact gcttcttgtt cccaaccttt acactcaata ctcaaacctt tttttgcttg
     1621 aatttttctc ataaaattcg ctcctgttct tttaagaagt taattccact aaatttaaaa
     1681 cgcttacatt attatcttca atattcatta tagtatgtta aaatatagcc aacaaatata
     1741 aataaactaa ttatccatag cttgaatcta taaataaaag gagcaaaaca catgaaaatt
     1801 attcagttag aatacttctt ggctatcgtg aaatataata gttttactaa agctgcacaa
     1861 tttttacata ttagccagcc atctttaact gctacgatta aaaaaatgga agcagattta
     1921 ggttatgact tatttacacg ttcaacaaaa gacatcaaga ttaccgaaaa aggaatacag
     1981 ttttatcgtt atgcgagcga attagttcaa caatatcgat ccacgatgga aaaaatgtat
     2041 gatttaagcg ttacatcaga accaaggata aaaattggga ctcttgaatc tacgaatcaa
     2101 tggattgcga atttaattcg aaagcaccat tccgactacc ctgaacagca atatcgttta
     2161 tatgaaatac atgataaaca tcaatctata gagcaattac tgaattttaa tattcattta
     2221 gctataacaa atgaaaaaat aacccacgaa gatataagat ccattccttt atatgaggaa
     2281 tcttacattt tattagcacc caaggaaaca tttaaaaatc aaaattgggt agatgttgaa
     2341 aatttgccac tcatattacc aaacaaaaat tctcaagtgc gcaaacactt agatgactat
     2401 tttaatagaa gaaatattcg tccaaatgtc gttgtagaaa cagatcgatt cgaatcagca
     2461 gttggatttg ttcatctcgg cttaggttac gctatcattc cgagatttta ttaccaatca
     2521 tttcacacgt ctaatttaga atataaaaaa attcgtccaa acttaggccg aaaaatttat
     2581 atcaattacc ataaaaaacg caaacactcc gaacaagtac atacattcgt acaacaatgc
     2641 caagattatt tatatggact tttagaggct ctttaactta agttattaga gcctcttatg
     2701 cagttgcgca gatcatcgta taaaaattaa tgacgtcatt tcaaaaatcg atacaaaaat
     2761 aatttattat aaaaattcta agaaagaagt gaagcagatg ttaaaatcta ttaatcatat
     2821 atgcttttca gtcagaaatt taaacgattc aatacatttt tatagagata ttttacttgg
     2881 gaaattgcta ttgactggta aaaaaactgc ttattttgag cttgcaggcc tatggattgc
     2941 tttaaatgaa gaaaaagata taccacgtaa tgaaattcac ttttcatata cacatatagc
     3001 tttcactata gatgacagcg aatttaaata ttggcatcag aggttaaaag ataataacgt
     3061 gaatatttta gaaggaagag ttagagatat tagagataga caatcaattt actttaccga
     3121 ccctgatggt cataagctag aattacatac tggcacactt gagaacagat taaattatta
     3181 taaagaggct aaaccacata tgacatttta caaataaggt gtcattataa aaaggcctct
     3241 tgaactccgt taaaatttta attaattatt atataataag agaacttttc aaacaataca
     3301 gt
//
LOCUS       DANJFZ010000296         3167 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.187, whole genome shotgun sequence.
ACCESSION   DANJFZ010000296 DANJFZ010000000
VERSION     DANJFZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.187"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            122..757
                     /locus_tag="PWN45_002949"
                     /pseudo
     CDS             122..757
                     /locus_tag="PWN45_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="PWN45_002950"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="PWN45_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL0624623.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="PWN45_002951"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="PWN45_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0624624.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="PWN45_002952"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="PWN45_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1065 a          497 c          722 g          883 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaat
//
LOCUS       DANJFZ010000297         2313 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.209, whole genome shotgun sequence.
ACCESSION   DANJFZ010000297 DANJFZ010000000
VERSION     DANJFZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.209"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            39..416
                     /locus_tag="PWN45_002953"
                     /pseudo
     CDS             39..416
                     /locus_tag="PWN45_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="PWN45_002954"
                     /pseudo
     CDS             413..1294
                     /locus_tag="PWN45_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..>2313
                     /locus_tag="PWN45_002955"
     CDS             1410..>2313
                     /locus_tag="PWN45_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0624625.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFED"
BASE COUNT          881 a          314 c          417 g          701 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag act
//
LOCUS       DANJFZ010000298         1566 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.233, whole genome shotgun sequence.
ACCESSION   DANJFZ010000298 DANJFZ010000000
VERSION     DANJFZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.233"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            73..1425
                     /gene="tet(38)"
                     /locus_tag="PWN45_002956"
     CDS             73..1425
                     /gene="tet(38)"
                     /locus_tag="PWN45_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDL0624626.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          490 a          231 c          269 g          576 t
ORIGIN      
        1 acaacacctg aggaaaggga acgtgcattt acagatatga ttgaaattgc actgtcattg
       61 gtgtagatga ttatgaatgt tgaatattct aaaataaaga aagcagtacc tattttatta
      121 ttcttatttg tattcagttt ggttatagac aactcattta aattgatttc tgtagccatt
      181 gctgatgact taaacatatc tgtaacgaca gtaagttggc aagcgacatt agccggttta
      241 gtaattggta ttggcgctgt agtatacgct tcattatctg atgccattag tatacgcaca
      301 ctatttattt atggcgtgat attaatcatt atcggatcaa ttattggtta cattttccaa
      361 catcaattcc cattactttt agttggacgt attattcaaa ctgccggttt agctgctgca
      421 gagacattat atgtgatata tgttgcaaag tatctttcta aagaggacca gaagacttac
      481 cttggcttaa gtacgagcag ttattccttg tcattagtta tcggtacatt atcaggtgga
      541 tttatttcta cgtatttaca ctggacaaat atgtttttaa ttgcattaat cgtagtattt
      601 acgttgccat tcctatttaa attattacca aaagaaaata atacgaataa agctcattta
      661 gattttgttg gcttaattct agtggcaact attgctacaa cagtcatgct gtttattacg
      721 aactttaatt ggttatatat gattggtgcc ttaattgcga ttatcgtttt tgcgctatat
      781 attaaaaatg cgcaacgtcc attagtaaat aaatcatttt tccaaaataa acgttatgct
      841 tcatttttat ttatagtatt tgtaatgtat gctatccaat tgggttatat ttttacgttc
      901 ccattcataa tggagcaaat ttatcatctg caactagaca caacatcact gttattagta
      961 ccgggttata tagtagcagt cattgttggt gcactaagtg gtaaaatcgg cgaatatctg
     1021 aattcaaaac aagcgattat cacagcaatt attttaatag cactgagctt gattttacct
     1081 gcatttgcag taggtaatca catttcaatc ttcgtcattt ctatgatatt ctttgcaggt
     1141 agctttgctt taatgtatgc acctttactt aacgaagcca ttaaaacaat agatcttaat
     1201 atgacaggtg tggctattgg tttttataat ttaattatta atgtggcggt atctgtaggt
     1261 attgcgattg ctgcggctct aatcgatttt aaagcattaa atttcccagg caatgatgca
     1321 ttaagttcac atttcggtat tattttaatt attttaggtt taatgagtat tgtcggatta
     1381 gttttattcg tcagcttaaa tcgttggaca caatctgaaa aataaataga tataaattcg
     1441 cgagatatat tcgtatttat agtaaaatta aataaagaga ttatataaca cgaggagtag
     1501 taagtatgaa atttgagaaa tatatagatc acactttatt gaagcctgag tcaacacgta
     1561 cgcaaa
//
LOCUS       DANJFZ010000299         1553 bp    DNA     linear   BCT 21-FEB-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate F207D5
            SAMN17866825-rid17224693.guided.235, whole genome shotgun sequence.
ACCESSION   DANJFZ010000299 DANJFZ010000000
VERSION     DANJFZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17866825
            Sequence Read Archive: SRR13689513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637085.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637085.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 00:30:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 33 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 19 of 69
            Pseudo Genes (multiple problems)  :: 12 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17866825-rid17224693.guided.235"
                     /isolate="F207D5"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Canada: Alberta"
                     /lat_lon="51.0447 N 114.0719 W"
                     /collection_date="2004"
                     /collected_by="APL"
     gene            91..1077
                     /locus_tag="PWN45_002957"
     CDS             91..1077
                     /locus_tag="PWN45_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDL0624627.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1049..1309
                     /locus_tag="PWN45_002958"
     CDS             1049..1309
                     /locus_tag="PWN45_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDL0624628.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1300..>1553)
                     /locus_tag="PWN45_002959"
     CDS             complement(1300..>1553)
                     /locus_tag="PWN45_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL0624629.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          558 a          214 c          256 g          525 t
ORIGIN      
        1 ccttatataa gactacattt gtagtatatt acaaatgtag tatttatgtc aaaataatgt
       61 tataattttt gtgatatgga ggtgtagaag gtgttatcat cttttttaat gttaagtata
      121 atcagttcat tgctcacgat atgtgtaatt tttttagtga gaatgctcta tataaaatat
      181 actcaaaata ttatgtcaca taagatttgg ttattagtgc tcgtctccac gttaattcca
      241 ttaataccat tttacaaaat atcgaatttt acattttcaa aagatatgat gaatcgaaat
      301 gtatctgaca cgacttcttc ggttagtcat atgttagatg gtcaacaatc atctgttacg
      361 aaagacttag caattaatgt taatcagttt gagacctcaa atataacgta tatgattctt
      421 ttgatatggg tatttggtag tttgttgtgc ttattttata tgattaaggc attccgacaa
      481 attgatgtta ttaaaagttc gtcattggaa tcgtcatatc ttaatgaacg acttaaagta
      541 tgtcaaagta agatgcagtt ctacaaaaag catataacaa ttagttatag ttcaaacatt
      601 gataatccga tggtatttgg tttagtgaaa tcccaaattg tactaccaac tgtcgtagtc
      661 gaaaccatga atgacaaaga aattgaatat attattctac atgaactatc acatgtgaaa
      721 agtcatgact taatattcaa ccagctttat gttgttttta aaatgatatt ctggtttaat
      781 cctgcactat atataagtaa aacaatgatg gacaatgact gtgaaaaagt atgtgataga
      841 aacgttttaa aaattttgaa tcgccatgaa catatacgtt atggtgaatc gatattaaaa
      901 tgctctattt taaaatctca gcacataaat aatgtggcag cacaatattt actaggtttt
      961 aattcaaata ttaaagaacg tgttaagtat attgcacttt atgattcaat gcctaaacct
     1021 aatcgaaaca agcgtattgt tgcgtatatt gtatgtagta tatcgagctt cacatgaaac
     1081 agctaaagaa gctttgggcg ataaagagtt aagagccatt gcacatgagt taactaaaac
     1141 agttaaggat aacatgagtg ttgattggtc taaacgagac agtgctaaag ctaaaatgag
     1201 agttcaagtt agacgcctat taaagaaata tggctatcca ccagatcttc aaaaaatggc
     1261 tgtggaacaa gttgtagagc aagcagaatt aatggcaagt cagcaataaa aaaataaatc
     1321 ataatgagtc cgggacataa agttcttgga taagtgaaaa aagacaattt ctattgaaat
     1381 aatatagaaa ttgtcttttt tataaatttt ttgattattt tcagctcgtt gagctactac
     1441 ttttcttata ttaagtgcca ttaatacaaa accaagttct cttttgactt tattgagtcc
     1501 tcggacagac atccgagtga aacccaaaat agccttcata aatccaaaaa cag
//
